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Sample records for actinomycetes aislados desde

  1. Estudio de actinomicetos marinos aislados de la costa central del Perú y su actividad antibacteriana frente a Staphylococcus aureus Meticilina Resistentes y Enterococcus faecalis Vancomicina Resistentes Study of marine actinomycetes isolated from the central coast of Peru and their antibacterial activity against Methicillin-Resistant Staphylococcus aureus and Vancomycin-Resistant Enterococcus faecalis

    Jorge León

    2011-06-01

    Full Text Available Objetivos. Determinar el potencial antimicrobiano de actinomicetos marinos frente a cepas S. aureus meticilino-resistentes (MRSA y E. faecalis vancomicina-resistentes (VRE. Materiales y métodos. En dos medios de cultivo se sembraron 29 cepas de actinomicetos aislados de sedimento marino. Se evaluó la capacidad inhibitoria mediante pruebas de antagonismo in vitro para MRSA y VRE. Se procesó los extractos orgánicos de tres actinomicetos seleccionados para determinar la Concentración Mínima Inhibitoria (CMI del compuesto activo. Resultados. La mayoría de los actinomicetos aislados correspondieron a un grupo homogéneo de blanco-grisáceos (62% con buen nivel de crecimiento en agar marino. Los porcentajes inhibitorios fueron superiores a 85% para ambos patógenos con halos de inhibición mayores a 69 y 78 mm de diámetro para MRSA y VRE respectivamente. Los extractos diclorometánicos de tres de los actinomicetos aislados (I-400A, B1-T61, M10-77 mostraron gran potencial inhibitorio de ambos patógenos, siendo M10-77 la cepa de actinomiceto de mayor actividad antibiótica frente a S. aureus ATCC 43300 resistente a meticilina y E. faecalis ATCC 51299 resistente a vancomicina con una Concentración Mínima Inhibitoria (CMI de 7,9 y 31,7 μg/ mL respectivamente. El análisis filogenético de la cepa M10- 77 presenta un 99% de similaridad con la especie marina Streptomyces erythrogriseus. Conclusiones. El sedimento marino de la costa central del Perú es fuente promisorio de cepas de actinomicetos con gran capacidad de producir compuestos bioactivos capaces de inhibir patógenos tipificados como multidrogo-resistentes tales como S. aureus meticilino resistentes y E. faecalis vancomicina resistentes.Objectives. To determine the antimicrobial potential of marine actinomycetes against drug-resistant pathogens represented by strains of methicillin-resistant Staphylococcus aureus (MRSA and vancomycin-resistant Enterococcus faecalis (VRE. Materials and

  2. Diversity of foam producing nocardioform actinomycetes isolated from biological foam from activated sludge plants in Comunidad Valenciana; Diversidad de actinomicetos nocardioformes productores de espumas biologicas aislados de plantas depuradoras de aguas residuales de la Comunidad Valenciana

    Soler, A.; Alonso, J.L.; Cuesta, G.

    2009-07-01

    The formation of biological foams in activated sludge systems is one of the most important problems of solid separation in wastewater treatment plants. Nocardioform actinomycetes are the most important filamentous bacteria responsible of foam formation. This group of microorganisms has hydrophobic cellular surfaces due to the mycolic acids. These foams interfere in wastewater treatment process because retain many suspended solids, block conductions and produce overflowing in the digesters and corridors. To identify correctly the nocardioform actinomycetes we have to do poli phasic taxonomy that includes 16S rDNA sequences analysis, determinate several chemo taxonomic markers and some phenotypic tests. (Author) 18 refs.

  3. Efecto Antagónico in vitro de Actinomicetos Aislados de Purines de Chipaca (Bidens pilosa L. Frente a Phytophthora infestans (Mont de Bary In vitro Antagonistic Effect of Actinomycetes Isolated from Chipaca (Bidens pilosa L. Purins Against Phytophthora infestans (Mont de Bary

    Yudy Astrid Fonseca Ardila

    2011-12-01

    Full Text Available Se estudió el efecto inhibidor de los actinomicetos presentes en purines o extractos fermentados de plantas de chipaca (Bidens pilosa L., sobre el crecimiento de Phytophthora infestans (Mont de Bary, causante del tizón tardío de la papa. Se elaboraron cuatro purines de flores, raíces, hojas-tallos y su mezcla. De estos purines se obtuvieron 25 aislamientos de actinomicetos, cada uno de los cuales se enfrentó con P. infestans en placas de medio de cultivo, utilizando la técnica de anillos de Gauze y estableciendo las concentraciones iniciales de esporas mediante conteos microscópicos en cámara de Neubauer. Los actinomicetos no crecieron en el purin de flores debido, posiblemente, a que en él no se utiliza suelo rizosférico o porque su pH (9 es mayor que el rango normal de crecimiento de estos microorganismos ( pH 6 -; 8. Se evidenció inhibición del crecimiento del oomycete por parte de 8 aislamientos de actinomicetos con porcentajes de inhibición entre 33,3 - 77,8%, provenientes de los purines de raíces, tallos-hojas y mezcla de partes de la planta. La mayor inhibición se obtuvo en los aislamientos AC001, AC010, AC011 y AC025 con conteos de 0,4, 6,0, 3,0, y 3,6 x10(5 esporas mL-1.Purins or liquid fermented extracts of chipaca (Bidens pilosa L. were prepared to establish the inhibitory effect of the actinomycetes found in such biopharmaceutical preparations on the growth of Phytophthora infestans (Mont de Bary, the causative of potato late blight disease. Four purins made from flowers, roots, leaf-steams and a mixture of them were prepared; 25 actinomycete isolates were obtained from these purins and their ability to resist challenge by P. infestans was ascertained in medium plates using the ring Gauze technique and establishing initial concentrations of spores by microscopic counting in Neubauer chamber. Actinomycetes did not grow in flower purin as rhizosphere soil was not used in its preparation or because this particular pH (9

  4. Natural Products from Mangrove Actinomycetes

    Dong-Bo Xu; Wan-Wan Ye; Ying Han; Zi-Xin Deng; Kui Hong

    2014-01-01

    Mangroves are woody plants located in tropical and subtropical intertidal coastal regions. The mangrove ecosystem is becoming a hot spot for natural product discovery and bioactivity survey. Diverse mangrove actinomycetes as promising and productive sources are worth being explored and uncovered. At the time of writing, we report 73 novel compounds and 49 known compounds isolated from mangrove actinomycetes including alkaloids, benzene derivatives, cyclopentenone derivatives, dilactones, macr...

  5. Bioactive compounds from marine actinomycetes

    Solanki, Renu; Khanna, Monisha; Lal, Rup

    2008-01-01

    Actinomycetes are one of the most efficient groups of secondary metabolite producers and are very important from an industrial point of view. Among its various genera, Streptomyces, Saccharopolyspora, Amycolatopsis, Micromonospora and Actinoplanes are the major producers of commercially important biomolecules. Several species have been isolated and screened from the soil in the past decades. Consequently the chance of isolating a novel actinomycete strain from a terrestrial habitat, which wou...

  6. Actinomycetes bioactivos de sedimento marino de la costa central del Perú

    Jorge León

    2013-04-01

    Full Text Available En el presente trabajo evaluamos la actividad antibacteriana y antifúngica de actinomycetes marinos sobre patógenos de origen clínico. Asimismo, fueron evaluadas la capacidad de producir enzimas extracelulares como carbohidrasas, lipasas y proteasas. Los Actinomycetes fueron aislados de sedimentos colectados entre setiembre a diciembre del 2005 de las Bahías de Ancón (Lima e Independencia (Ica de 34 y 100 m de profundidad. El aislamiento se realizó en Agar Caseína - Almidón (ACA y Agar Marino (AM con adición de Cicloheximide (10 μg/mL. Las evaluaciones antimicrobianas fueron realizadas frente a bacterias patógenas antibiótico-multirresistentes y hongos de origen clínico; en tanto, para evaluar su actividad multienzimática se utilizaron sustratos poliméricos diversos. Se aislaron un total de 62 actinomycetes, de los cuales 31 (50% mostraron actividad antibacteriana frente a Staphylococcus aureus, 36 (59% frente a Pseudomonas aeruginosa y 23 (37% a ambos patógenos. Las cepas de actinomycetes I-400A y M10-77 identificadas en cada caso como Streptomyces y Thermoactinomyces fueron las que exhibieron mayor actividad inhibitoria frente a P. aeruginosa y S. aureus respectivamente. Asimismo, 13 actinomycetes (20,97% mostraron actividad antifúngica frente a cultivos de Candida albicans cepa 1511 y 17 (27,42% frente a Candida albicans cepa 1511MIC; sin embargo, ningún actinomycete presentó actividad inhibitoria frente a Aspergillus niger, Aspergillus fumigatus y Trichophyton mentagrophytes. La mayoría de los actinomycetes mostraron tener actividad multienzimática capaz de hidrolizar compuestos poliméricos como el tween-80 (96%, la gelatina (95%, almidón (93%, lecitina (88% y la caseína (74%. Extractos del compuesto activo obtenidos de la cepa M10-77 con etil acetato rindieron notable actividad inhibitoria contra S. aureus. Se concluye que el sedimento marino es fuente de Actinomycetes con gran capacidad de producir sustancias

  7. A variety of rare actinomycetes in soils

    Actinomycetes are an integral part of a microbic complex of soil. Long time distribution actinomycetes in soils judged by quantity streptomyces, but working out of selective methods of allocation have allowed to reveal actinomycetes of rare sorts Saccharopolyspora, Nocardia etc. in soils to make their ecological characteristics

  8. Diversity of Soil Actinomycetes in Yunnan, China

    Xu, L.; Li, Q.; Jiang, C

    1996-01-01

    Since 1978, about 4,200 soil samples have been collected from 22 selected areas of various vegetational and climatic types throughout the province of Yunnan. Actinomycetes of 29 genera were isolated by the methods employed. The correlations between diversity and climate were grouped into tropical, subtropical plateau, cool temperate mountain, and snowy mountain types. Actinomycete populations of the first two types were more complex than were the other ones. Correlations between actinomycete ...

  9. Antitumor compounds from marine actinomycetes.

    Salas, José A.; Carmen Méndez; Carlos Olano

    2009-01-01

    Chemotherapy is one of the main treatments used to combat cancer. A great number of antitumor compounds are natural products or their derivatives, mainly produced by microorganisms. In particular, actinomycetes are the producers of a large number of natural products with different biological activities, including antitumor properties. These antitumor compounds belong to several structural classes such as anthracyclines, enediynes, indolocarbazoles, isoprenoides, macrolides, non-ribosomal pept...

  10. Actinomycetes and Fungi in Surface Waters and in Potable Water

    Niemi, R. Maarit; Knuth, Sisko; Lundström, Kenneth

    1982-01-01

    In Finnish lakes and rivers used as water supplies, mesophilic fungi and actinomycetes were common, whereas thermophilic fungi and actinomycetes were present only in low concentrations. Fungi and actinomycetes were more abundant in eutrophic and mesotrophic lakes than in oligotrophic lakes. River water contained more thermophilic actinomycetes and fungi and mesophilic actinomycetes than did lake water. Runoff from soil seemed to be an important factor contributing to the incidence of these mi...

  11. Therapeutically Active Biomolecules from Marine Actinomycetes

    Mani Jayaprakashvel

    2012-09-01

    Full Text Available For the past few centuries, the biological sources of terrestrial origin have been explored and exploited for bioactive metabolites. This has resulted in the stagnancy of discovering either novel compounds or compounds with novel bioactivities. Thus, researchers across the globe have started exploring our big Oceans, for the search of bioactive metabolites. During the past few decades, the research on bioactive metabolites from marine biological resources has geared up and among the sources marine actinomycetes are proved to be best. Marine actinomycetes, the filamentous bacteria from marine environment have been intensively studied for bioactive metabolites. The biological diversity of marine actinomycetes was found to be enormous, thanks to culture dependent and culture independent biodiversity approaches. This great diversity of marine actinomycetes has offered greater chemical diversity. The diverse chemical compounds of marine actinomycetes have been found to have various biological activities such as antimicrobial, anti-tumor, anti-malarial, anti-algal, antioxidant, anti-inflammatory etc. These various bioactive metabolites of marine actinomycetes are having scope for developing as potent therapeutic agents. The potential of marine actinomycetes is rightly realized though the current biological wealth of these organisms isrelatively unexplored.

  12. Isolation of Cellulolytic Actinomycetes from Marine Sediments

    Veiga, Manuel; Esparis, Azucena; Fabregas, Jaime

    1983-01-01

    The cellulolytic activity of 36 actinomycetes strains isolated from marine sediments was investigated by the cellulose-azure method. Approximately 50% of the isolates exhibited various degrees of cellulolytic activity.

  13. Novel method for selective isolation of actinomycetes.

    Hirsch, C F; Christensen, D L

    1983-01-01

    A new technique for the selective isolation of actinomycetes from natural mixed microbial populations is described. A nutrient agar medium was overlaid with a 0.22- to 0.45-microns-pore cellulose ester membrane filter, and the surface of the filter was inoculated. During incubation, the branched mycelia of the actinomycetes penetrated the filter pores to the underlying agar medium, whereas growth of nonactinomycete bacteria was restricted to the filter surface. The membrane filter was removed...

  14. NOVEL BIOACTIVE COMPOUNDS FROM MANGROVE DERIVED ACTINOMYCETES

    Kumari Amrita; Jain Nitin; Subathra Devi, C.

    2012-01-01

    Mangrove is most productive and unexplored ecosystem that approximately covers one fourth of world coastline with high diversity of thriving organism. Recently the rate of isolation of novel bioactive compounds from microorganism living in mangrove forest has tremendously increased which is reflected in significant hasten for exploration of mangrove actinomycetes. Actinomycetes are group of bacteria which are extremely interesting as active producers of many primary and secondary metabolites....

  15. ENZYMATIC ACTIVITY OF FRESH WATER ACTINOMYCETES

    Gunda Madan Mohan; Singara Charya, M. A.

    2012-01-01

    Fresh water systems represent a largely untapped source for isolation of novel microorganisms. Gram-positive actinomycetes are of special interest, since they are known to produce chemically diverse compounds with wide range of biological activities. Twenty four actinomycetes with distinct characteristics were isolated from three freshwater systems of Karimnagar, Andhra Pradesh, India viz: Lower Manair Dam, Manakondur Pond and Kothapally Pond. These isolates were screened for their antagonist...

  16. Elicitation of secondary metabolism in actinomycetes

    Abdelmohsen, Usama Ramadan; Grkovic, Tanja; Balasubramanian, Srikkanth; Kamel, Mohamed Salah; Quinn, Ronald J.; Hentschel, Ute

    2015-01-01

    Genomic sequence data have revealed the presence of a large fraction of putatively silent biosynthetic gene clusters in the genomes of actinomycetes that encode for secondary metabolites, which are not detected under standard fermentation conditions. This review focuses on the effects of biological (co-cultivation), chemical, as well as molecular elicitation on secondary metabolism in actinomycetes. Our review covers the literature until June 2014 and exemplifies the diversity of natural prod...

  17. Exposure of water consumers to mesophilic actinomycetes.

    Ojanen, T H; Katila, M. L.; Mäntyjärvi, R.; Seppänen, H.; Muittari, A; Kuusisto, P.; Virtanen, P.

    1983-01-01

    In autumn 1978 an epidemic of respiratory disease resembling allergic alveolitis occurred in a small Finnish community. The disease was caused by repeated exposures to tap water aerosol. The raw water of the community and the sand filters of the purification system were heavily contaminated with mesophilic actinomycetes. Fourteen different strains of actinomycetes were isolated. Exposed persons with and without symptoms as well as unexposed control persons were tested for antibodies against f...

  18. ISOLATION AND DIVERSITY OF ACTINOMYCETES IN THE CHESAPEAKE BAY

    Chesapeake Bay was investigated as a source of actinomycetes to creen for production of novel bioactive compounds. he presence of relatively large populations of actinoplanetes, chemotype IID actinomycetes in Chesapeake Bay sediment samples indicates that is an eminently suitable...

  19. Isolation and Diversity of Actinomycetes in the Chesapeake Bay †

    Takizawa, Masayuki; Colwell, Rita R.; Hill, Russell T.

    1993-01-01

    Chesapeake Bay was investigated as a source of actinomycetes to screen for production of novel bioactive compounds. The presence of relatively large populations of actinoplanetes (chemotype II/D actinomycetes) in Chesapeake Bay sediment samples indicates that it is an eminently suitable ecosystem from which to isolate actinomycetes for screening programs. Actinomycetes were isolated from sediment samples collected in Chesapeake Bay with an isolation medium containing nalidixic acid, which pro...

  20. Actinomycetes in the rhizosphere of semidesert soils of Mongolia

    Norovsuren, Zh.; Zenova, G. M.; Mosina, L. V.

    2007-04-01

    The population density of actinomycetes in the desert-steppe soil, rhizosphere, and the above-ground parts of plants varies from tens to hundreds of thousands of colony-forming units (CFU) per gram of substrate. The actinomycetal complexes of the brown desert-steppe soil without plant roots are more diverse in their taxonomic composition than the actinomycetal complexes in the rhizosphere and the aboveground parts of plants. Additionally to representatives of the Streptomyces and Micromonospora genera, actinomycetes from the Nocardia, Saccharopolyspora, Thermomonospora, and Actinomadura genera were identified in the soil. The population density of actinomycetes in the rhizosphere and in the soil reached hundreds of thousand CFU/g; it considerably exceeded the population density of actinomycetes in the aboveground parts of plants. The maximum population density of actinomycetes was determined in the rhizosphere of Asparagus gobicus, Salsola pestifera, and Cleistogenes songorica.

  1. Actividad fibrolítica de hongos ruminales aislados de ecosistemas tropicales

    Gualdrón Duarte, Laura; Mayorga, O; Rodríguez, Diego Alberto; Manovacía, Patricia; Martín, Alejandro; Carulla Fornaguera, Juan Evangelista; Barahona, Rolando

    2009-01-01

    Se evaluó la capacidad de degradación de sustratos altamente fibrosos por 18 aislados fungales ruminales en fermentaciones de 120 horas utilizando como única fuente de carbono pasto colosuana (Bothriochloa pertusa). Dentro del ensayo se incluyeron aislados pertenecientes a los géneros Neocallimastix, Orpinomyces y Piromyces. De acuerdo con los parámetros del modelo de Gompertz, la fase de adaptación de los aislados fue de 23,8 horas (h) ± 4,20, con una producción de gas promedio de 195 ml/g s...

  2. Isolation of actinomycetes from cervical specimens.

    Traynor, R M; Parratt, D; Duguid, H L; Duncan, I D

    1981-01-01

    Cervical smears from 30 women were examined. Fifteen of these had actinomycetes visible by direct smear examination and the organisms were isolated in 13. Of the remainder, all had negative smears and culture was similarly negative in 12, whilst in 3, the cultures were either positive (1) or suspicious (2).

  3. Aerobic and microaerophilic actinomycetes of typical agropeat and peat soils

    Zenova, G. M.; Gryadunova, A. A.; Pozdnyakov, A. I.; Zvyagintsev, D. G.

    2008-02-01

    A high number (from tens of thousands to millions of CFU/g of soil) of actinomycetes and a high diversity of genera were found in typical peat and agropeat soils. Agricultural use increases the number and diversity of the actinomycete complexes of the peat soils. In the peat soils, the actinomycete complex is represented by eight genera: Streptomyces, Micromonospora, Streptosporangium, Actinomadura, Microbispora, Saccharopolyspora, Saccharomonospora, and Microtetraspora. A considerable share of sporangial forms in the actinomycete complex of the peat soils not characteristic of the zonal soils was revealed. The number of actinomycetes that develop under aerobic conditions is smaller by 10-100 times than that of aerobic forms in the peat soils. Among the soil actinomycetes of the genera Streptomyces, Micromonospora, Streptosporangium, Actinomadura, Microbispora, and Microtetraspora, the microaerophilic forms were found; among the Saccharopolyspora and Saccharomonospora, no microaerophilic representatives were revealed.

  4. PRODUCTION AND CHARACTERIZATION OF ANTIBIOTICS FROM SOIL-ISOLATED ACTINOMYCETES

    Vengadesh prabhu K; Sundaramoorthi C; Gupta Saurabh; Karthick K; Tamilselvi N

    2011-01-01

    In the present study, the soil samples were collected from various places in the Coimbatore, near drainage, mud and coloured soil where there were no plants around. Actinomycetes strains were isolated in specific medium using soyabean casein digest (SBCD) medium. These Actinomycetes were screened with regard to potential against Gram-positive and Gram-negative bacteria. The purified Actinomycetes strains were performed in biochemical tests. Crowded plate technique was used for the isolation o...

  5. Distribution of actinomycetes in near-shore tropical marine sediments.

    Jensen, P R; Dwight, R; Fenical, W.

    1991-01-01

    Actinomycetes were isolated from near-shore marine sediments collected at 15 island locations throughout the Bahamas. A total of 289 actinomycete colonies were observed, and all but 6 could be assigned to the suprageneric groups actinoplanetes and streptomycetes. A bimodal distribution in the actinomycete population in relation to depth was recorded, with the maximum numbers occurring in the shallow and deep sampling sites. This distribution can be accounted for by a rapid decrease in strepto...

  6. Actinomycetes as host cells for production of recombinant proteins

    Tamura Tomohiro; Mitani Yasuo; Nakashima Nobutaka

    2005-01-01

    Abstract Actinomycetes (Actinobacteria) are highly attractive as cell factories or bioreactors for applications in industrial, agricultural, environmental, and pharmaceutical fields. Genome sequencing of several species of actinomycetes has paved the way for biochemical and structural analysis of important proteins and the production of such proteins as recombinants on a commercial scale. In this regard, there is a need for improved expression vectors that will be applicable to actinomycetes....

  7. SCREENING OF HMG CO A REDUCTASE INHIBITOR PRODUCING MARINE ACTINOMYCETES

    SRINU, PHANI BHUSHAN,MOGES, SRILAKSHMI, SANKAR, PRABHAKAR,LAKSHMINARAYANA

    2013-01-01

    The objective of the present study was screening of 3-hydroxy-3- methyl glutaryl Co A (HMG CoA) reductase inhibitor producing marine actinomycetes. A total of 65 morphologically different actinomycetes were screened for HMG CoA reductase inhibitor production in a two stage submerged fermentation and evaluated for HMG CoA reductase inhibitor activity by agar diffusion and thin layer chromatography technique using lovostatin as a control. Among 65 marine Actinomycete strains, only one strain pr...

  8. Fermentative production of carotenoids from marine actinomycetes

    B Ashokkumar

    2009-12-01

    Full Text Available Background and Objectives: In marine actinomycetes, carotenoid production occurs in constitutive, light-dependent or cryptic manner. The present work deals with the fermentative production of carotenoids from marine actinomycetes."nMaterials and Methods: Marine actinomycetes namely Streptomyces strain AQBMM35 was isolated from the marine sponge Mycale mytilorum collected from South West coast of India using ISP media. The Streptomyces isolates were characterized for their colony characteristics, morphological properties, physiological and biochemical properties and were tentatively identified. Fermentation of the strain under fluorescent white light was carried out for the production of carotenoids. UV spectrum, TLC and HPLC analysis were done for the confirmation of carotenoids."nResults: The characteristics studied strongly suggest that the strain AQBMM35 belongs to the genus Streptomyces sp. It has been found that Streptomyces strain (AQBMM35 fermenting under fluorescent white light produced carotenoids. Spectrophotometric analysis of the carotenoid fraction revealed a peak at 280 nm. TLC analysis of the carotenoid extract showed the presence of phytoene (Rf of 0.81. HPLC confirmed the production of phytoene when compared with standards."nConclusion: The fermenting sponge-associated Streptomyces isolate (AQBMM35 produced carotenoids namely phytoene. If this symbiotic Streptomyces strain, from which secondary metabolite like carotenoids are derived, can be cultured under light, then it can be used for mass production of precursor pigment and it can be used as an antioxidant and also as a food additive.

  9. CARACTERIZACIÓN FENOTÍPICA DE AISLADOS DE RIZOBIOS PROCEDENTES DE LA LEGUMINOSA FORRAJERA Canavalia ensiformis

    Ionel Hernández Forte

    2012-01-01

    Full Text Available El objetivo del presente trabajo fue caracterizar fenotípicamente aislados de rizobios procedentes de la leguminosa forrajera Canavalia ensiformis. Se caracterizaron doce aislados, cinco posibles miembros del género Rhizobium/ Sinorhizobium, siete posibles integrantes del género Bradyrhizobium y dos cepas comerciales, BR 2001 y BR 2003a. Para la caracterización fenotípica de los aislados bacterianos se estudió su capacidad de utilizar seis fuentes de carbono, la tolerancia a condiciones de acidez y a diferentes niveles de cloruro de sodio (NaCl, la resistencia a seis antibióticos así como su crecimiento en tres temperaturas de incubación. Se determinó además la capacidad de estos aislados de producir polihidroxibutiratos (PHB. Todos los aislados estudiados utilizaron la lactosa, glucosa y manitol como fuentes de carbono. Uno de los aislados de rápido crecimiento resultó el más tolerante a pH 4.5. Los aislados posibles miembros del género Rhizobium/ Sinorhizobium toleraron una mayor concentración de NaCl que los posibles integrantes del género Bradyrhizobium. Tres de los aislados de rápido crecimiento fueron resistentes a los seis antibióticos utilizados. Cuando los aislados fueron incubados a 370C todos, con excepción del el aislado CP3 y la cepa comercial BR 2001, presentaron un crecimiento similar al control. El aislado CP10, de lento crecimiento, tuvo la capacidad de producir las mayores concentraciones de PHB (0.023 g.L-1.

  10. Malate dehydrogenases from actinomycetes: structural comparison of Thermoactinomyces enzyme with other actinomycete and Bacillus enzymes.

    Smith, K.; Sundaram, T K; Kernick, M

    1984-01-01

    Malate dehydrogenases from bacteria belonging to the genus Thermoactinomyces are tetrameric, like those from Bacillus spp., and exhibit a high degree of structural homology to Bacillus malate dehydrogenase as judged by immunological cross-reactivity. Malate dehydrogenases from other actinomycetes are dimers and do not cross-react with antibodies to Bacillus malate dehydrogenase.

  11. ACTINOMYCETES: TOLERANCE AGAINST HEAVY METALS AND ANTIBIOTICS

    Smriti Singh, Shruti Pandey and Hotam Singh Chaudhary*

    2014-09-01

    Full Text Available Heavy metals can be both, essential as well as toxic for living beings. Micronutrients such as, Co, Fe, Mn have important role to play in living systems whereas, Pb Cd etc. pose harmful effects even at low concentrations. When these heavy metals get accumulated within the tissues of the organisms at various levels of the ecological chain, they cause decrease in the biomass and biological diversity by affecting the growth, morphology and activity of the organisms. Accumulation of heavy metals in soil also causes soil contamination, which can be overcome with the help of bioremediation. A large group of soil bacteria belonging to the Actinomycetes species are exposed to heavy metals in a variety of ways; although, they show resistance to heavy metals. The species of actinomycetes possess resistance for antibiotic synthesis as well. This makes the actinomycetes suitable agents for bioremediation. In this experiment, a total of 20 isolates from Shivpuri region of Madhya Pradesh were tested for the metal tolerance against selected heavy metals. After this, the most tolerant strains were tested to check their antibiotic susceptibility. Metal tolerance was tested by agar well diffusion method and tube dilution method. Out of the 20 isolates, Ash1, Ash 2, Ash 4,Ash 6, Ash 7, Ash 8, Ash 9, Ash 10, Ash 11, Ash 12, Ash 13, Ash 15 were resistant at 10 mM conc. of CuSo4, but their growth was inhibited at higher concentrations of metal salts. Isolates Ash 10, Ash 11, Ash 12, Ash 13, Ash 19, Ash 20 were found to be resistant at 10mM conc. of ZnSO4, but they were also inhibited at higher concentrations. For different concentrations of Pb(CH3COO2 most of the isolates showed same level of tolerance.

  12. Actinomycetes in garden soils of the city of Kirov

    Shirokikh, I. G.; Solov'eva, E. S.; Ashikhmina, T. Ya.

    2013-05-01

    The population density, diversity, and structure of the actinomycetic complexes were studied in garden soils of the city of Kirov. The relationships between the structure of the complexes and the acidity, the concentrations of the mobile forms of heavy metals, and the soil humus content were analyzed. The specific features of the actinomycetic population in the garden soils of the city in comparison with the transport ecotopes and suburban territories were revealed. It was demonstrated that the actinomycetic complexes in the garden soils preserve their structural similarity with the actinomycetic complexes of the suburban forest parks despite certain changes in the composition of the dominant species and the relative abundance of the separate taxa. The obtained data indicate that the garden plots in the city contribute to the preservation of ecologically balanced ecosystems.

  13. Consorcios microbianos con actividad ácido-láctica promisoria aislados desde inoculantes bacterianos nativos para ensilajes

    Byron Leoncio Díaz-Monroy; Arabel Elías Iglesias; Elaine Valiño-Cabrera

    2015-01-01

    El objetivo de este trabajo fue aislar y caracterizar cepas de bacterias ácido lácticas (BAL) a partir de preparados microbianos (PM) nativos elaborados a base de residuos agroindustriales, como suero de leche, estiércol bovino y contenido ruminal, y diseñados como inoculantes biológicos para procesos de ensilaje de residuales orgánicos poscosecha. Se evaluaron 5 tratamientos (preparados microbianos, PM + 33% de residuo inoculante), con 3 réplicas: 1) Tratamiento control sin inoculante (PMSI)...

  14. Consorcios microbianos con actividad ácido-láctica promisoria aislados desde inoculantes bacterianos nativos para ensilajes

    Byron Leoncio Díaz-Monroy

    2015-04-01

    Full Text Available El objetivo de este trabajo fue aislar y caracterizar cepas de bacterias ácido lácticas (BAL a partir de preparados microbianos (PM nativos elaborados a base de residuos agroindustriales, como suero de leche, estiércol bovino y contenido ruminal, y diseñados como inoculantes biológicos para procesos de ensilaje de residuales orgánicos poscosecha. Se evaluaron 5 tratamientos (preparados microbianos, PM + 33% de residuo inoculante, con 3 réplicas: 1 Tratamiento control sin inoculante (PMSI, 2 Suero de leche (PMSL, 3 Estiércol bovino (PMEB, 4 Combinación de suero+estiércol (PMM y 5 Contenido ruminal (PMCR; además, en todos se adicionó: melaza (20%, urea (1%, sal mineral (1% y agua (45%. En recipientes plásticos se fermentaron tapados durante 96 horas, a 14 °C; de allí se obtuvieron 3 muestras de 500 mL de cada PM, se sembraron en agar MRS (Man, Rogosa y Sharpe y se incubaron a 37 ºC durante 72 h bajo condiciones anaerobias (AnaeroGen, Oxoid. Las cepas de BAL son catalasa y oxidasa negativas, cocos, coco-bacilos y bacilos Gram positivos no esporulados. La identificación bioquímica se realizó con el sistema API-50CHL (BioMérieux, France y la base de datos APILAB Plus versión 3.3.3. Se aislaron 39 cepas de BAL, 4 del PMSI (2 Lactobacillus plantarum, 1 L. brevis y 1 Lactococcus lactis, 12 del PMSL (3 Lactobacillus rhamnosus, 3 L. casei, 2 L. acidophilus, 2 L. paracasei y 2 L. fermentum, 7 del PMEB (3 Streptococcus bovis, 2 Lactobacillus plantarum y 2 Leuconostoc mesenteroides, 10 del PMM (3 Lactobacillus casei, 2 L. plantarum, 2 L. acidophilus, 1 L. fermentum, 1 L. rhamnosus y 1 L. paracasei y 6 del PMCR (2 Lactobacillus vitulinus, 2 L. ruminus, 1 L. reuteri y 1 Pediococcus damnosus. En conclusión, la mejor fuente de cepas de BAL fue PMSL, que le otorga ventajas como posible inoculante bacteriano para ensilajes. En todos los PM se identificaron cepas homofermentativas y heterofermentativas, asociadas en consorcios microbianos; se generó una alternativa tecnológica para aprovechar tres residuos agroindustriales.

  15. Site-Specific Recombination Strategies for Engineering Actinomycete Genomes

    Herrmann, Simone; Siegl, Theresa; Luzhetska, Marta; Petzke, Lutz; Jilg, Caroline; Welle, Elisabeth; Erb, Annette; Leadlay, Peter F; Bechthold, Andreas; Luzhetskyy, Andriy

    2012-01-01

    The feasibility of using technologies based on site-specific recombination in actinomycetes was shown several years ago. Despite their huge potential, these technologies mostly have been used for simple marker removal from a chromosome. In this paper, we present different site-specific recombination strategies for genome engineering in several actinomycetes belonging to the genera Streptomyces, Micromonospora, and Saccharothrix. Two different systems based on Cre/loxP and Dre/rox have been ut...

  16. The medically important aerobic actinomycetes: epidemiology and microbiology.

    McNeil, M. M.; Brown, J. M.

    1994-01-01

    The aerobic actinomycetes are soil-inhabiting microorganisms that occur worldwide. In 1888, Nocard first recognized the pathogenic potential of this group of microorganisms. Since then, several aerobic actinomycetes have been a major source of interest for the commercial drug industry and have proved to be extremely useful microorganisms for producing novel antimicrobial agents. They have also been well known as potential veterinary pathogens affecting many different animal species. The medic...

  17. Antibiotic Drug Discovery from the New Marine Actinomycete Genus Marinomyces

    Fenical, William H.

    2007-01-01

    Actinomycetes are high G+C content Gram-positive bacteria with an unparalleled ability to produce diverse secondary metabolites. These bacteria, which are best known from soils, have been studied extensively by the pharmaceutical industry and account for a disproportionately large amount of the $25.3 billion annual global sales of microbially derived pharmaceuticals. In recent years however, the yield of new lead compounds from common soil-derived actinomycetes has diminished significantly,...

  18. ISOLATION OF ANTIBACTERIAL COMPOUND FROM MARINE SOIL ACTINOMYCETES

    2012-01-01

    The antibacterial study revealed that the isolated marine soil actinomycetes by crowded plate method and was identified by MIDI Advance technology (gas chromatographic) method and Thus, the microbes were identified according to their fatty acid sequences. The actinomycetes species were identified as Streptomyces rimosus, Streptomyces fradiae, and Streptomyces griseoflavus and the antibacterial assay was carried out by well diffusion method. Out of these only Streptomyces rimosus having high a...

  19. Extremophilic and extremotolerant actinomycetes in different soil types

    Zenova, G. M.; Manucharova, N. A.; Zvyagintsev, D. G.

    2011-04-01

    Problems on the resistance of soil actinomycetes to various environmental factors (pH, salinity, temperature, and moisture) are discussed. Actinomycetes as a special group of prokaryotes were revealed to have a greater range of tolerance to these factors than was thought earlier. The regularities of the distribution of extremophilic and extremotolerant actinomycetes developing in unusual for mycelial bacteria conditions, their structural-functional characteristics, and their taxonomic composition were determined. The predominance of acidophilic representatives of the Micromonospora genus in acid soils (typical peat, soddy-podzolic, and taiga podzol) and the haloalkaliphilic Streptomyces pluricilirescens and S. prunicolor species in desert saline soils are shown. The specific features of the actinomycete complexes on thermal fields of the weakly developed stratified volcanic soils are described. In these complexes, the thermophilic forms were represented only by species of the Micromonospora genus; and the mesophilic forms, by Microbispora species. In the periodically heated desert soils, among the thermophilic actinomycetes, representatives of rare Actinomadura, Saccharopolyspora and Streptosporangium genera along with Streptomyces species were indicated. The mechanisms of the resistance of the actinomycetes to the extreme environmental conditions are discussed.

  20. Moderately haloalkaliphilic actinomycetes in salt-affected soils

    Zvyagintsev, D. G.; Zenova, G. M.; Oborotov, G. V.

    2009-12-01

    It was found that the population density of actinomycetes in solonchaks and saline desert soils varied from hundreds to tens of thousands of colony-forming units (CFUs) per 1 g of soil depending on soil type and was by 1-3 orders of magnitude lower than the number of mycelial bacteria in main soil types. Actinomycetes grow actively in saline soils, and the length of their mycelium reaches 140 m per 1 g of soil. Domination of moderately halophilic, alkaliphilic, and haloalkaliphilic actinomycetes, which grow well under 5% NaCl and pH 8-9, is a specific feature of actinomycetal complexes in saline soils. Representatives of Streptomyces and Micromonospora genera were found among the haloalkaliphilic actinomycetes. Micromonospores demonstrated lower (than streptomycetes) adaptability to high salt concentrations. Investigation of the phylogenetic position of isolated dominant haloalkaliphilic strains of streptomycetes performed on the basis of sequencing of the gene 16S rRNA enabled identifying these strains as Streptomyces pluricolorescens and S. prunicolor.

  1. Antibacterial activity of some actinomycetes from Tamil Nadu, India

    Pachaiyappan Saravana Kumar; John Poonga Preetam Raj; Veeramuthu Duraipandiyan; Savarimuthu Ignacimuthu

    2012-01-01

    Objective:To isolate novel actinomycetes and to evaluate their antibacterial activity. Methods:Three soil samples were collected from Vengodu (village) in Kanchipuram district, Tamil Nadu, India. Actinomycetes were isolated using serial dilution and plating method on actinomycetes isolation agar. Results: Totally 35 isolates were obtained on the basis of colony characteristics on actinomycetes isolation agar. All the isolates were screened for antibacterial activity by cross streak method. Medium and optimization of day were done for the potent strains using Nathan's agar well diffusion method. Isolation of bioactive compounds from significant active isolates was done by using different media. The most active isolate VAS 10 was identified as Actinobacterium Loyola PBT VAS 10 (accession No. JF501398) using 16s rRNA sequence method. The hexane, ethyl acetate, dichloromethane and butanol extracts of VAS 10 were tested against bacteria. The maximum antibacterial activity was observed in dichloromethane and ethyl acetate;maximum zones of inhibition were observed against Enterococcus durans. The rRNA secondary structure and the restriction sites of Actinobacterium Loyola VAS 10 were predicted using Genebee and NEBCutter online tools respectively. Conclusions: The present study showed that among the isolated actinomycetes, Actinobacterium Loyola PBT VAS 10 (accession No. JF501398) showed good antibacterial activity against the tested bacteria.

  2. ISOLATION OF ENDOPHYTIC ACTINOMYCETES FROM MEDICINAL PLANTS AND ITS MUTATIONAL EFFECT IN BIOCONTROL ACTIVITY

    Hema Shenpagam N.*, D. Kanchana Devi ** and Sinduja G.

    2012-01-01

    In the present study, the endophytic actinomycetes were collected from three medicinal plants Azadiracta indica, Ocimum sanctum and Phyllanthus amarus. Endophytic actinomycetes were isolated using different media like Starch casein agar, Starch casein nitrate agar, Actinomycetes isolation agar and Soyabean agar, while it showed more colonies in Starch casein agar. The endophytic actinomycetes were stained and biochemical tests were performed. Antimicrobial compound was purified from the filtr...

  3. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes

    Tong, Yaojun; Charusanti, Pep; Zhang, Lixin;

    2015-01-01

    facilitate the genetic manipulation of actinomycetes, we developed a highly efficient CRISPR-Cas9 system to delete gene(s) or gene cluster(s), implement precise gene replacements, and reversibly control gene expression in actinomycetes. We demonstrate our system by targeting two genes, actIORF1 (SCO5087) and...... actVB (SCO5092), from the actinorhodin biosynthetic gene cluster in Streptomyces coelicolor A3(2). Our CRISPR-Cas9 system successfully inactivated the targeted genes. When no templates for homology-directed repair (HDR) were present, the site-specific DNA double-strand breaks (DSBs) introduced by Cas9....... Moreover, we developed a system to efficiently and reversibly control expression of target genes, deemed CRISPRi, based on a catalytically dead variant of Cas9 (dCas9). The CRISPR-Cas9 based system described here comprises a powerful and broadly applicable set of tools to manipulate actinomycetal genomes....

  4. Reflexiones desde la Trinchera

    Verónica Valenzuela; Ana B. Uribe; Karla Y. Covarrubias; Ma. Guadalupe Chávez Méndez; María Teresa Quinto; Ana Josefina Cuevas

    2007-01-01

    El hilo conductor de los textos de esta sección está formado por las reflexiones de colaboradoras estrechas de la revista, desde sus mismas entrañas y recovecos. Verónica Valenzuela, primera coordinadora editorial de ESCC, rememora la aventura (y la hazaña) de iniciar una revista académica hace dos décadas desde Colima. Ana B. Uribe recupera sus experiencias como segunda coordinadora editorial para repensar su formación académica al interior del Programa Cultura. Karla Y. Co...

  5. ISOLATION OF ENDOPHYTIC ACTINOMYCETES FROM MEDICINAL PLANTS AND ITS MUTATIONAL EFFECT IN BIOCONTROL ACTIVITY

    Hema Shenpagam N.*, D. Kanchana Devi ** and Sinduja G.

    2012-11-01

    Full Text Available In the present study, the endophytic actinomycetes were collected from three medicinal plants Azadiracta indica, Ocimum sanctum and Phyllanthus amarus. Endophytic actinomycetes were isolated using different media like Starch casein agar, Starch casein nitrate agar, Actinomycetes isolation agar and Soyabean agar, while it showed more colonies in Starch casein agar. The endophytic actinomycetes were stained and biochemical tests were performed. Antimicrobial compound was purified from the filtrate by ethanol extraction method. Antagonistic activities of endophytic actinomycetes isolates were tested against bacterial pathogens (Staphylococcus aureus, Streptococcus pyogenes, Klebsiella pneumoniae and Pseudomonas aeruginosa and the fungi Rhizopus. For the selected isolates antibiotic resistance was checked using various antibiotic discs like Amoxycillin, Penicillin, Rifampicin and Ampicillin. The strains which showed efficient antibacterial activity were selected to study the effect of mutation by physical and chemical method. In this study, UV mutated endophytic actinomycetes increase antibiotic production than non-mutated endophytic Actinomycetes, whereas in chemical mutation it does not increase the antibiotic production.

  6. Comparing actinomycete and bacterial soil and sediment communities for metagenomics

    Hill, P.; Krištůfek, Václav; Feijoo, A. M.; Caballero, S.; van Elsas, D.

    Praha: Výzkumný ústav rostlinné výroby, Praha, 2005. s. 10. [Život v pode /6./. 01.02.2005-02.02.2005, Praha] Institutional research plan: CEZ:AV0Z60660521 Keywords : actinomycete * bacterial soil and sediment communities * metagenomics Subject RIV: EH - Ecology, Behaviour

  7. A New Degraded Sesquiterpene from Marine Actinomycete Streptomyces sp. 0616208

    Xiu Chao XIE; Wen Li MEI; You Xing ZHAO; Kui HONG; Hao Fu DAI

    2006-01-01

    A new degraded sesquiterpene was isolated from the marine actinomycete Streptomyces sp. 0616208. Its structure was elucidated as (1α, 4aα, 5α, 7β, 8aβ)-5, 8a-dimethyl-decahydrona-phthalene-1, 4a, 7-triol on the basis of spectroscopic data.

  8. Antibiotics production by an actinomycete isolated from the termite gut.

    Matsui, Toru; Tanaka, Junichi; Namihira, Tomoyuki; Shinzato, Naoya

    2012-12-01

    As well as the search for new antibiotics, a new resource or strains for the known antibiotics is also important. Microbial symbionts in the gut of termites could be regarded as one of the feasible resource for such purpose. In this study, antibiotic-producing actinomycetes were screened from symbionts of the termite gut. 16SrRNA sequence analysis for the 10 isolates revealed that they belong to actinomycetes such as Streptomyces sp., Kitasatospora sp., and Mycobacterium sp. A culture broth from one of the isolate, namely strain CA1, belonging to the genera Streptomyces exhibited antagonistic activity against actinomycetes (Micrococcus spp.), gram-positive bacteria (Bacillus spp.), and yeast (Candida spp.). The structures of 2 compounds isolated from the culture broth of the strain CA1 were identified as those of actinomycin X2 and its analog, D. This study is the first to report that some symbionts of the termite gut are antibiotic-producing actinomycetes, and suggest that the termite gut is a feasible resource for bioprospecting. PMID:22359219

  9. Benzoquinona e hidroquinona preniladas y otros constituyentes aislados de piper bogotense c. dc.

    Peña, Luz Angela; Avella, Eliseo; Puentes de Díaz, Aura María

    2010-01-01

    Dos compuestos, que innovan la química conocida del género Piper, (2'E,6'E)-2- famesilhidroquinona, (2'E,6'E)-2-famesil- 1,4-benzoquinona, y a-D-glucosa fueron aislados de los frutos de Piper bogotense C. DC; los tallos y las hojas presentaron, como constimyentes mayoritarios, lactama del ácido 10-amino-4-hidroxi-2,3-dimetoxifenantreno carboxílico, 1,2-metilendioxi- 6-metil-4H-dibenzo lde,g] quinolina- ,5(6H)-diona (Cefaradiona A), estigmasterol y sitosterol. Adicionalmente de las ho...

  10. Benzoquinona e hidroquinona preniladas y otros constituyentes aislados de Piper Bogotense C. DC.

    Luz Angela Peña; Eliseo Avella; Aura María Puentes de Díaz

    2010-01-01

    Dos compuestos, que innovan la química conocida del género Piper, (2'E,6'E)-2- famesilhidroquinona, (2'E,6'E)-2-famesil- 1,4-benzoquinona, y a-D-glucosa fueron aislados de los frutos de Piper bogotense C. DC; los tallos y las hojas presentaron, como constimyentes mayoritarios, lactama del ácido 10-amino-4-hidroxi-2,3-dimetoxifenantreno carboxílico, 1,2-metilendioxi- 6-metil-4H-dibenzo lde,g] quinolina- ,5(6H)-diona (Cefaradiona A), estigmasterol y sitosterol. Adicionalmente de las ho...

  11. Actividad antiinflamatoria de d-amirona y 4, 7-dimetoxiapigenina aislados de alnus acuminata

    Salama, Ahmed; Avendaño, Inés Yamile

    2009-01-01

    El presente trabajo determinó el efecto antiinflamatorio de d-amirona (olean-13(18)-en-3-ona) y 4',7-dimetoxiapigenina (5-hidroxi-4¢,7-dimetoxiflavona), aislados de Alnus acuminata (Betulaceae), por el método del edema plantar en ratas hembra, en dosis de 30, 60 y 100 mg/kg y de 30, 60 y 80 mg/kg respectivamente. Ambas sustancias mostraron una actividad antiinflamatoria significativa. El efecto más alto de d-amirona se presentó a la primera hora en las tres dosis ensayadas comparable con el e...

  12. Marine actinomycetes from Madeira Archipelago preliminary taxonomic studies

    Ilda Santos Sanches

    2014-06-01

    Full Text Available The oceans cover 70 % of the Earth´s surface and harbor most of the planet´s biodiversity. However the microbiological component of this diversity remains relatively unexplored. Marine actinomycetes, are a robust resource of chemically prolific novelty. Producing structurally unique biological active secondary metabolites, generating a valuable source for innovative biotechnology and drug discovery[1,2]. As a consequence, the ecological role of actinomycetes and their marine ecosystems may no longer be neglected. It is crucial to move our research efforts into ocean regions for which we know little or nothing about the indigenous microbial diversity. The Portuguese Archipelago, Madeira is located in the Macaronesian Atlantic region, emerging from the African tectonic plate, found in the extreme south of the Tore-Madeira ridge, has a unique biogeography and biodiversity. These distinctive characteristics combined with the fact that Madeira have never been explored, as far as indigenous marine actinomycetes are concerned, makes it from the scientific point of view, the perfect target for our studies. From 662 marine sediment samples collected along Madeira Archipelago (Figure 1 during June of 2012, covering depths from 10-1310 m, a total of 421 actinomycete strains were isolated. In a previous study, an assemblage of 82 strains was selected for taxonomic identification, having into account representative morphological diversity characteristics of the actinomycetes, isolated from Madeira Archipelago. Based on 16S rRNA gene sequencing, it was observed that the genera Streptomyces, Micromonospora and Salinispora were predominant, 81% [3]. Additionally, in a recent study, our team selected 168 strains with Salinispora look-alike morphological features. From these 28 strains were identified as belonging to the seawater-obligate marine actinomycete genus Salinispora. Representing the first report of Salinispora spp. in the Macaronesian Atlantic Ocean

  13. Distribution of actinomycetes in oil contaminated ultisols of the Niger Delta (Nigeria)

    2000-01-01

    The distribution of actinomycetes in oil contaminated sandy loam ultisols of the Niger Delta region of Nigeria was studied to aid in understanding the effect of hydrocarbons on indigenous microbial population in tropical soils. The average total counts of actinomycetes in all the oil samples analysed was 103 cfu/g. Higher counts of actinomycetes were observed during the dry season than during the wet season. The counts of hydrocarbonoclastic actinomycetes correlated positively with the total count of actinomycetes.The actinomycetes were generally restricted to the top soil(0-10 cm soil depth) although a seemingly deeper(down to 40 cm soil depth) distribution was noticed in the dry season. The isolates included oil degrading species of Actinoplanes, Norcadia,Streptomyces and Streptosporangium. Their high oil utilization ability indicates their positive potential and role in the bioremediation of oil-spilled soils.

  14. Competitive biosorption of thorium and uranium by actinomycetes

    The competitive biosorption of thorium and uranium by actinomycetes was examined. Of the actinomycetes tested, Streptomyces levoris showed the highest ability to sorb both thorium and uranium from aqueous systems. Thorium sorption was not affected by co-existed uranium, while uranium sorption was strongly hindered by co-existed thorium. The amounts of both thorium and uranium sorbed by Streptomyces levoris cells increased with an increase of the solution pH. Although the equilibrium isotherm of uranium biosorption is in similar manner as that of thorium biosorption, uranium was sorbed much faster than thorium. Biosorption isotherm of each metal ion could be well fitted by Langmuir isotherm taking the ionic charge of metal ions into account. The Langmuir isotherm for binary system did not explain completely the competitive biosorption of thorium and uranium by Streptomyces levoris. However, the results suggested that the ion species of both metals in the cells should be Th(OH)22+ and UO22+, respectively. (author)

  15. Actinomycetes for Marine Drug Discovery Isolated from Mangrove Soils and Plants in China

    Hong, Kui; Gao, An-Hui; Xie, Qing-Yi; Gao, Hao Gao; Zhuang, Ling; Lin, Hai-Peng; Yu, Hai-Ping; Li, Jia; Yao, Xin-Sheng; Goodfellow, Michael; Ruan, Ji-Sheng

    2009-01-01

    The mangrove ecosystem is a largely unexplored source for actinomycetes with the potential to produce biologically active secondary metabolites. Consequently, we set out to isolate, characterize and screen actinomycetes from soil and plant material collected from eight mangrove sites in China. Over 2,000 actinomycetes were isolated and of these approximately 20%, 5%, and 10% inhibited the growth of Human Colon Tumor 116 cells, Candida albicans and Staphylococcus aureus, respectively, while 3%...

  16. MINIATURIZED FERMENTATION IN EPPENDORF TUBES FOR THE DETECTION OF ANTAGONISTIC ACTINOMYCETES

    2012-01-01

    Actinomycetes are recognized as a prolific source for bioactive metabolites. Screening adequate number of strains by appropriate high quality screening protocol determines success of drug discovery programs. To accomplish screening for antagonistic actinomycetes at higher rate, the fermentation stage of the initial screening needs to be miniaturized. The present study is attempted for miniaturized production of bioactive compounds from 40 actinomycetes isolated from less explored ecosystems. ...

  17. Terrestrial actinomycetes from diverse locations of Uttarakhnad, India: Isolation and screening for their antibacterial activity

    Vijay Kumar; Gajraj Singh Bisht; Omprakash Gusain

    2013-01-01

    Background and Objective Uttarakhand region is less explored, but possess a great biodiversity. This diversity can be explored for isolation and characterization of new actinomycetes strains for seeking antimicrobial molecules. It can therefore be predicted that novel bioactive metabolite producing actinomycetes can be discovered to combat multidrug resistant bacterial pathogens. Materials and Methods Variations in the viable count of actinomycetes were accessed in different altitudes. Actino...

  18. THE ANTAGONISTIC ACTIVITY OF ACTINOMYCETES OF STREPTOMYCES GENUS IN RELATION TO TRICHODERMA KONINGII

    Barbara Breza-Boruta; Zbigniew Paluszak

    2016-01-01

    The aim of the study was to estimate the effect of actinomycetes of genus Streptomyces on the growth of the antagonistic fungus Trichoderma koningii. 150 strains of Streptomyces spp. isolated from two potato cropping systems were used to the tests. Analyses were conducted experimentally in vitro on PDA medium with pH 6 and 7. The results obtained clearly indicate the inhibitory effect of actinomycetes on the fungus T. koningii. Of the tested population of actinomycetes only two strains did no...

  19. Isolation and Characterization of Medically Important Aerobic Actinomycetes in Soil of Iran (2006 - 2007)

    Aghamirian, Mohammad Reza; Ghiasian, Seyed Amir

    2009-01-01

    The aerobic actinomycetes are a large group of soil-inhabiting bacteria that occur worldwide. Some of them are the main cause of two important diseases, nocardiosis and actinomycetoma. To identify the prevalence and geographic distribution of aerobic actinomycetes in soil of Qazvin province, a study was carried out during 2006-2007. In this study, the incidence and diversity of medically important aerobic actinomycetes was determined in 300 soil samples of different parts of Qazvin. The suspe...

  20. An N-acyl homolog of mycothiol is produced in marine actinomycetes

    Newton, Gerald L.; Fahey, Robert C.; Jensen, Paul R.; Fenical, William; MacMillan, John B

    2008-01-01

    Marine actinomycetes have generated much recent interest as a potentially valuable source of novel antibiotics. Like terrestrial actinomycetes the marine actinomycetes are shown here to produce mycothiol as their protective thiol. However, a novel thiol, U25, was produced by MAR2 strain CNQ703 upon progression into stationary phase when secondary metabolite production occurs and became the dominant thiol. MSH and U25 were maintained in a reduced state during early stationary phase but become ...

  1. Actinomycetes from Red Sea Sponges: Sources for Chemical and Phylogenetic Diversity

    Usama Ramadan Abdelmohsen; Chen Yang; Hannes Horn; Dina Hajjar; Timothy Ravasi; Ute Hentschel

    2014-01-01

    The diversity of actinomycetes associated with marine sponges collected off Fsar Reef (Saudi Arabia) was investigated in the present study. Forty-seven actinomycetes were cultivated and phylogenetically identified based on 16S rRNA gene sequencing and were assigned to 10 different actinomycete genera. Eight putatively novel species belonging to genera Kocuria, Mycobacterium, Nocardia, and Rhodococcus were identified based on sequence similarity values below 98.2% to other 16S rRNA gene sequen...

  2. Exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea

    M. Sreevidya; Gopalakrishnan, S.; H. Kudapa; Varshney, R. K.

    2016-01-01

    Abstract The main objective of the present study was to isolate and characterize actinomycetes for their plant growth-promotion in chickpea. A total of 89 actinomycetes were screened for their antagonism against fungal pathogens of chickpea by dual culture and metabolite production assays. Four most promising actinomycetes were evaluated for their physiological and plant growth-promotion properties under in vitro and in vivo conditions. All the isolates exhibited good growth at temperatures f...

  3. STUDIES ON ANTIMICROBIAL ACTIVITY OF ACTINOMYCETES AGAINST MDR WOUND BACTERIAL ISOLATES

    L.Ashokkumar; Balagurunathan, R.; P. Palanivel; D. Jegadeeshkumar

    2012-01-01

    A total of five different actinomycete isolates were recovered from mine soil samples collected from Salem, Tamilnadu. These were then assessed for their antibacterial activity against five multidrug resistance bacterial wound isolates. All five isolates of actinomycete exhibited antagonistic activity. The zone of inhibition ranged between 11-25 mm. Among the 5 isolates of actinomycetes A5 isolate has highest antibacterial activity against S.aureus and E.coli. Out of five bacterial isolates P...

  4. Isolation, Phylogenetic Analysis and Anti-infective Activity Screening of Marine Sponge-Associated Actinomycetes

    Safwat Ahmed; Ute Hentschel; Mona Radwan; Abou-El-Ela, Soad H.; Amro Hanora; Pimentel-Elardo, Sheila M.; Usama Ramadan Abdelmohsen

    2012-01-01

    Terrestrial actinomycetes are noteworthy producers of a multitude of antibiotics, however the marine representatives are much less studied in this regard. In this study, 90 actinomycetes were isolated from 11 different species of marine sponges that had been collected from offshore Ras Mohamed (Egypt) and from Rovinj (Croatia). Phylogenetic characterization of the isolates based on 16S rRNA gene sequencing supported their assignment to 18 different actinomycete genera representing seven diffe...

  5. Comparison of methods for isolation and enumeration of thermophilic actinomycetes from dust.

    Treuhaft, M W; Arden Jones, M P

    1982-01-01

    Thermophilic actinomycetes are the primary sensitizing agents in farmer's lung disease. We compared dilution pour-plate and spread-plate methods for their usefulness in enumerating thermophilic actinomycetes in moldy silage dust and evaluated the ability of a nonquantitative gravity settling technique to recover thermophilic actinomycetes from moldy silage. Spread plates and pour plates yielded similar estimates of total thermophiles. Higher counts were observed on spread plates (P less than ...

  6. Diversity of Aquatic Actinomycetes in Lakes of the Middle Plateau, Yunnan, China

    Jiang, C; Xu, L.

    1996-01-01

    A total of 749 sediment and water samples were collected from 12 lakes of the Middle Plateau of Yunnan from 1983 to 1993. The diversity and biological characteristics of the aquatic actinomycetes in these lakes were studied. Sixteen genera of actinomycetes were isolated from these samples. Micromonospores assumed a notable dominance (from 39 to 89%) in the actinomycete populations of these lake sediments. Streptomycetes were the second most abundant organisms. The diversity and counts of acti...

  7. Isolation and characterization of marine-derived actinomycetes with cytotoxic activity from the Red Sea coast

    Mohamed Saleh Abdelfattah; Mohammed Ismail Youssef Elmallah; Usama Wahid Hawas; Lamia Taha Abou El-Kassema; Mennat Allah Gamal Eid

    2016-01-01

    Objective: To isolate and evaluate the cytotoxic activity of different actinomycetes species isolated from the Red Sea coast in Sharm el-Sheikh, Egypt. Methods: Forty actinomycetes strains were isolated from different sediments and seawater samples collected from the Red Sea coast in Egypt. Actinomycetes were recognized by morphological and microscopic examinations. Cell viability and cytotoxicity induced by the crude extracts on breast cancer cell lines MDA-MB-231 were assessed using meth...

  8. Ecological features of rare actinomycetes genera in soils of Azerbaijan

    A. R. Abushova; S. A. Gasanova; M. A. Kasimzade

    2010-01-01

    The results of the research of rare species of actinomycetes in some soils of Azerbaijan were studied. On the selective media the representatives of Streptomyces, Streptosporangium, Micromonospora, Actinomadura, Microbispora, Saccharopolyspora and Saccharomonospora were revealed. Species of Streptomyces and Micromonospora dominate in mountain-wood ecosystems (60 %.). It was established that among streptomyces species of Cinereus section of Violaceus series; Helvolo-Flavus sections of Helvolus...

  9. Molecular insights on the biosynthesis of antitumour compounds by actinomycetes

    Olano Álvarez, Carlos; Méndez Fernández, María del Carmen; Salas Fernández, José Antonio

    2011-01-01

    Summary Natural products are traditionally the main source of drug leads. In particular, many antitumour compounds are either natural products or derived from them. However, the search for novel antitumour drugs active against untreatable tumours, with fewer side‐effects or with enhanced therapeutic efficiency, is a priority goal in cancer chemotherapy. Microorganisms, particularly actinomycetes, are prolific producers of bioactive compounds, including antitumour drugs, produced as secondary ...

  10. A novel taxonomic marker that discriminates between morphologically complex actinomycetes

    Girard, Geneviève; Traag, Bjørn A; Sangal, Vartul; Mascini, Nadine; Hoskisson, Paul; Goodfellow, Michael; van Wezel, Gilles

    2013-01-01

    In the era when large whole genome bacterial datasets are generated routinely, rapid and accurate molecular systematics is becoming increasingly important. However, 16S ribosomal RNA sequencing does not always offer sufficient resolution to discriminate between closely related genera. The SsgA-like proteins are developmental regulatory proteins in sporulating actinomycetes, whereby SsgB actively recruits FtsZ during sporulation-specific cell division. Here, we present a novel method to classi...

  11. Lactose-reversible coaggregation between oral actinomycetes and Streptococcus sanguis.

    Kolenbrander, P. E.; Williams, B L

    1981-01-01

    Freshly isolated strains of oral actinomycetes were obtained from human dental plaque and were tested for the ability to coaggregate with common laboratory stock strains of Streptococcus sanguis. Strains belonging to the genera Actinomyces, Arachnia, Bifidobacterium, and Bacterionema were isolated. Only members of the genus Actinomyces coaggregated with the streptococci, and only Actinomyces viscosus and Actinomyces naeslundii exhibited lactose-reversible interactions. A total of 61 strains, ...

  12. Degradation of Lignin-Related Compounds by Actinomycetes

    Ball, A.S.; Betts, W. B.; McCarthy, A.J.

    1989-01-01

    Evidence for activity against the lignin fraction of straw was produced for a range of actinomycete strains. Decolorization of the polymeric dye Poly R and oxidation of veratryl alcohol, indicators of ligninolytic activity in white rot fungi, and utilization of fractionated Kraft lignin and low-molecular-weight methoxylated aromatic compounds were the criteria used. The relationships between these activities and the solubilization of native lignin are discussed.

  13. Actinomycetes in Karstic caves of northern Spain (Altamira and Tito Bustillo).

    Groth, I; Vettermann, R; Schuetze, B; Schumann, P; Saiz-Jimenez, C

    1999-05-01

    A variety of isolation procedures were carried out to study the involvement of bacteria in the colonisation and biodeterioration of Spanish caves with paleolithic rock art (Altamira and Tito Bustillo). The applied techniques mainly aimed to isolate heterotrophic bacteria such as streptomycetes, nocardioform and coryneform actinomycetes, and other gram-positive and gram-negative bacteria. The results demonstrated that actinomycetes were the most abundant gram-positive bacteria in the caves. Actinomycetes revealed a great taxonomic diversity with the predominant isolates belonging to the genus Streptomyces. Members of the genera Nocardia, Rhodococcus, Nocardioides, Amycolatopsis, Saccharothrix, Brevibacterium, Microbacterium, and coccoid actinomycetes (family Micrococcaceae) were also found. PMID:10353805

  14. The structural-functional organization of thermotolerant complexes of actinomycetes in desert and volcanic soils

    Zenova, G. M.; Kurapova, A. I.; Lysenko, A. M.; Zvyagintsev, D. G.

    2009-05-01

    It has been found that the number of thermotolerant actinomycetes in strongly heated soils of deserts and volcanic regions is comparable to or exceeds the number of mesophilic actinomycetes. Among the latter group, streptomyces usually predominate; among thermotolerant actinomycetes, representatives of the Micromonospora, Streptosporangium, Actinomadura, Saccharopolyspora, Microtetraspora, and Microbispora genera are identified. Thermotolerant actinomycetes display the full cycle of their development in these soils. The method of fluorescent in situ hybridization has made it possible to determine that mycelial forms predominate among the metabolically active representatives of Actinobacteria; their portion increases with the rise in the temperature of soil incubation.

  15. [Isolation and antimicrobial activities of actinomycetes from vermicompost].

    Wang, Xue-jun; Yan, Shuang-lin; Min, Chang-li; Yang, Yan

    2015-02-01

    In this paper, actinomycetes were isolated from vermicompost by tablet coating method. Antimicrobial activities of actinomycetes were measured by the agar block method. Strains with high activity were identified based on morphology and biochemical characteristics, as well as 16S rDNA gene sequence analysis. The results showed that 26 strains of actinomycetes were isolated, 16 of them had antimicrobial activities to the test strains which accounts for 61.54% of all strains. Among the 16 strains, the strain QYF12 and QYF22 had higher antimicrobial activity to Micrococcus luteus, with a formed inhibition zone of 27 mm and 31 mm, respectively. While the strain QYF26 had higher antimicrobial activity to Bacillus subtilis, and the inhibition zone diameter was 21 mm. Based on the identification of strains with high activity, the strain QYF12 was identified as Streptomyces chartreusis, the strain QYF22 was S. ossamyceticus and the strain QYF26 was S. gancidicus. This study provided a theoretical basis for further separate antibacterial product used for biological control. PMID:26137678

  16. ISOLATION AND ANTIMICROBIAL AND DEGRADATIVE POTENTIAL OF ACTINOMYCETES

    Padma Singh* and Vani Sharma

    2013-02-01

    Full Text Available Problem Statement: Does the soil Actinomycetes have Antimicrobial and Petrol degradation potential? It is an intriguing question. Actinomycetes are continues to be a subject of study with reference to their Antimicrobial and degradative potential. However studies have been done is limited. Our object was to study its Antimicrobial activity in wide spectrum and to study its degradation potential on Petrol. Approach: In this study we have isolated total 5 Actinomycetes from the Ganga river bed. All the isolates later purified and identified by various Morphological and Biochemical test. Here Nocardia was subjected to antimicrobial test against Streptococcus, Mucor and Aspergillus and it was also subjected to degradation test against Petrol. Result: The 5 isolates are Streptomyces, Micromonospora, Micromono sporangium and 2 different strain of Nocardia (Na1 and Na2. The 2 strains of Nocardia are active against Streptococcus (Na1 29.6mm, Na2 26.6mm, Mucor (Na1 12.5mm, Na2 22.5mm and Aspergillus (Na1 50%, Na2 60%. They also degrade Petrol very effectively, decrease in total organic carbon of the medium was observed during the degradation of petrol. Conclusion: Our observation provides us with evidence that these agents can be used for the production of new antibiotics and as the agent to control the environment pollution.

  17. ANTIBIOTIC RESISTANCE AND CHROMIUM REDUCTION PATTERN AMONG ACTINOMYCETES

    Preeti Jain

    2012-01-01

    Full Text Available Actinomycetes, one of the most important groups of microbes, exhibit many interesting activities such as degradation and transformation of organic and metal substrates together with production of antibiotics. With these bioactivities, actinomycetes would play an important role in the webs of the marine environment. The present study was designed to evaluate the antibiotic resistance pattern, antibiotic producing potential and chromium resistance as well as chromium reduction potential of a range of actinomycetes isolated from marine environments. Actinomycetes were isolated from marine sediment samples obtained from St. Martin’s Island in Bangladesh. Antibiotic resistance among the selected isolates was studied against 10 different antibiotics by disc diffusion method and antibiotic producing potential was assessed by the perpendicular streak method. The isolates were screened for resistance towards heavy metal Cr(VI on culture plates supplemented with Cr(VI at concentrations ranging from 1-5 mM of Cr(VI. Highly resistant isolates were subjected to screening for Cr(VI reduction activity, which was estimated using the Cr(VI specific colorimetric reagent 1, 5-diphenylcarbazide. Out of the total 30 different selected isolates, 25 (83.33% showed resistance against more than three antibiotics and 6 (20% showed resistance to more than six antibiotics. Ninety three percent of the isolates showed MAR index greater than 0.2 and tolerance to Cr(VI at 1mM of initial Cr(VI. None of the isolates displayed antimicrobial activity against the organisms tested. Among the isolates tested for chromate reduction, two were most efficient showing complete reduction of 1mM Cr(VI within 24 h. These two isolates (SM-11, SM-20 were capable of reducing chromate even at high initial Cr(VI concentrations. Remarkably, the isolate SM-11 was found to reduce 82.67%, 44.34% of Cr(VI at 2.5mM, 5mM of initial Cr(VI concentrations respectively, within 72h of incubation. The

  18. Airway inflammation among compost workers exposed to actinomycetes spores

    Kari Kulvik Heldal

    2015-05-01

    Full Text Available Objectives. To study the associations between exposure to bioaerosols and work-related symptoms, lung function and biomarkers of airway inflammation in compost workers. Materials and method. Personal full-shift exposure measurements were performed on 47 workers employed at five windrow plants (n=20 and five reactor plants (n=27. Samples were analyzed for endotoxins, bacteria, fungal and actinomycetes spores. Health examinations were performed on workers and 37 controls before and after work on the day exposure was measured. The examinations included symptoms recorded by questionnaire, lung function by spirometry and nasal dimensions by acoustic rhinometry (AR. The pneumoproteins CC16, SP-D and SP-A were measured in a blood sample drawn at the end of the day. Results. The levels of endotoxins (median 3 EU/m[sup]3[/sup] , range 0–730 EU/m[sup]3[/sup] and actinomycetes spores (median 0.2 × 10[sup]6[/sup] spores/m[sup]3[/sup] , range 0–590 × 10[sup]6[/sup] spores/m[sup]3[/sup] were significantly higher in reactor plants compared to windrow plants. However, windrow composting workers reported more symptoms than reactor composting workers, probably due to use of respiratory protection. Exposure-response relationships between actinomycetes spores exposure and respiratory effects, found as cough and nose irritation during a shift, was significantly increased (OR 4.3, 95% CI 1.1–16, OR 6.1, 95% CI 1.5–25, respectively, p<0.05 among workers exposed to 0.02–0.3 × 10[sup]6[/sup] actinomycetes spores/m 3 , and FEV1/FVC% decreased cross shift (b=–3.2, SE=1.5%, p<0.01. Effects were weaker in the highest exposed group, but these workers used respiratory protection, frequently limiting their actual exposure. No relationships were found between exposure and pneumoprotein concentrations. Conclusions. The major agent in the aerosol generated at compost plants was actinomycetes spores which was associated with work related cough symptoms and work

  19. Caracterización de actinobacterias raras, degradadoras de lignocelulosa: demostración de actividad lacasa en dos aislados de Tsukamurella sp y Cellulosimicrobium sp

    Enrique Luis Revollo Escudero

    2014-01-01

    Full Text Available Título en ingles: Characterization of lignocelluloses-degrading rare actinobacteria: Demostration of laccase activity in two isolates of Tsukamurella sp and Cellulosimicrobium sp Resumen: Las características fisicoquímicas de la lignina y su compactación con la celulosa han dificultado la explotación biotecnológica de enormes cantidades de biomasa vegetal. Las lacasas constituyen una subfamilia de oxidasas multicobre que intervienen en la despolimerización de la lignina. Si bien han sido ampliamente caracterizadas en los hongos, los estudios de la diversidad y las funcionalidades de las lacasas en los procariotas se han centrado especialmente en isoformas enzimáticas de Streptomyces sp. En este trabajo se aislaron 20 cepas de actinobacterias del suelo. La actividad lacasa de 17 de ellas fue evidenciada en ensayos cualitativos con guayacol y dos cepas seleccionadas fueron caracterizadas en detalle. Las pruebas morfológicas y el análisis de las secuencias del gen 16S rRNA apuntan a que estos dos aislados pertenecen a los géneros Tsukamurella y Cellulosimicrobium. En cultivo sumergido con agitación, AC01 (Tsukamurella sp. expresó una máxima actividad de oxidación de ABTS (2,2’-azino-bis-(3-etilbenzotiazolin-6-sulfonato de 108 U/L. Por otra parte, AC18 (Cellulosimicrobium sp. que había exhibido una actividad oxidativa de guayacol superior a las 16 cepas restantes y demostró ser resistente a niveles tóxicos de cobre, logró un valor máximo de oxidación del ABTS de 0,56 U/L. Estos resultados sugieren que en el aislado AC18 operaría un fenómeno de especificidad de sustrato o de inductor, regulador de la expresión y de la actividad lacasa cuantificable. La caracterización genómica y funcional de las lacasas de nuevas actinobacterias lignocelulósicas ampliará la gama de centros redox con aplicaciones biotecnológicas específicas, además de facilitar el establecimiento de sus relaciones evolutivas con las eucariotas

  20. FLAVONOIDES AISLADOS DE LAS INFLORESCENCIAS DE Piper hispidum Kunth (PIPERACEAE Y DERIVADOS ACETILADOS

    Luis Enrique Cuca

    2009-04-01

    Full Text Available A partir del extracto etanólico de las inflorescencias de la especie Piper hispidum Kunth (Piperaceae fueron aislados tres flavonoides: 5-hidroxi-7-metoxiflavanona, 5-hidroxi-4’,7-dimetoxiflavanona y 2’,4’,6’-trimetoxidihidrochalcona. De las flavanonas aisladas fueron obtenidos los  derivados acetilados: 5-acetoxi-7-metoxiflavanona y  5-acetoxi-4’,7-dimetoxiflavanona. Las estructuras fueron elucidadas empleando técnicas espectroscópicas y por comparación con datos de literatura. Los compuestos naturales y sus derivados fueron sometidos al bioensayo de letalidad frente a Artemia salina. El flavonoide 5-hidroxi-7-metoxiflavanona presenta la mayor actividad tóxica frente a los microcrustaceos CL50 1.8 µg/ml.

  1. ISOLASI DAN KARAKTERISASI ACTINOMYCETES-ASOSIASI SPONS PENGHASIL ANTIBIOTIK KOLEKSI PULAU BARRANG LOMPO MAKASSAR

    Rante, Herlina

    2012-01-01

    Actinomycetes merupakan jenis mikroorganisme yang sangat penting sebagai penghasil metabolit sekunder untuk pengobatan. Senyawa bioaktif dari mikroba diperoleh melalui serangkaian penapisan untuk menghasilkan suatu senyawa antibakteri. Penelitian ini bertujuan untuk mengisolasi dan mengindentifikasi bakteri actinomycetes dari spons yang berasal dari pulau Barranglompo Makassar dan mengisolasi dan mengidentifikasi senyawa antibakteri secara bioassay guided fractination Isolasi Ac...

  2. Bioactive Potential of Actinomycetes from Less Explored Ecosystems against Mycobacterium tuberculosis and Other Nonmycobacterial Pathogens

    Manikkam, Radhakrishnan; Venugopal, Gopikrishnan; Subramaniam, Balaji; Ramasamy, Balagurunathan; Kumar, Vanaja

    2014-01-01

    Bioactive potential of actinomycetes isolated from certain less explored Indian ecosystems against Mycobacterium tuberculosis and other nonmycobacterial pathogens was investigated. Actinomycetes were isolated from the soil samples collected from desert, coffee plantation, rubber forest, and hill area and their cultural and micromorphological characteristics were studied. Crude extracts were prepared by agar surface fermentation and tested against M. tuberculosis isolates by luciferase reporte...

  3. Screening of Actinomycetes From Lipar Area of Oman Sea to Investigate the Antibacterial Compounds

    Shams, MR; Shahnavaz; Ghazvini; Valinasab

    2015-01-01

    Background Actinomycetes are one of the most important sources for the production of antibacterial compounds. Marine environments, due to their unique characteristics, are considered a good option to search for bacteria with the capability of producing antimicrobial compounds. Objectives The purpose of this study was to isolate the actinomycetes producing antibacterial compounds. Materials and ...

  4. In Vitro Investigation of Antifungal Activities of Actinomycetes against Microsporum gypseum

    Naser Keikha

    2013-02-01

    Conclusion: The findings of the present research show that terrigenous actinomycetes have an antifungal effect upon Microsporum gypseum. So, one hopes that-in future-rather than administering antifungal chemicals that have side-effects, dermatophytic infections can be cured by applying these actinomycetes.

  5. STUDIES ON ANTIMICROBIAL ACTIVITY OF ACTINOMYCETES AGAINST MDR WOUND BACTERIAL ISOLATES

    L.Ashokkumar

    2012-12-01

    Full Text Available A total of five different actinomycete isolates were recovered from mine soil samples collected from Salem, Tamilnadu. These were then assessed for their antibacterial activity against five multidrug resistance bacterial wound isolates. All five isolates of actinomycete exhibited antagonistic activity. The zone of inhibition ranged between 11-25 mm. Among the 5 isolates of actinomycetes A5 isolate has highest antibacterial activity against S.aureus and E.coli. Out of five bacterial isolates Pseudomonas aeruginosa was highly suppressed by actinomycetes followed by E.coli. The maximum antibacterial activity was observed on 14th day incubation. The result of primary screening reveals that most of the active actinomycetes isolates were active against gram positive bacteria (S.aureus than gram negative bacteria. The antibiotic profile of these isolates underlined their potential as a source of novel antibiotics.

  6. Caerulomycin A- An antifungal compound isolated from marine actinomycetes.

    Ambavane, V.; Tokdar, P.; Parab, R.; Sreekumar, E.S.; Mahajan, G.B.; Mishra, P.D.; DeSouza, L.; Ranadive, P.

    of the most successful ap- proaches is to screen microbial resources to extract novel antifungal antibiotics. Drug discovery from natural products has been traditionally focused on empirical exploitation of the most prolific microbial groups: actinomycetes... obtained from mass, IR and 1H NMR spectra (Figures 3-5). In 1H NMR spectra, the signals at δ 7.8 and 7.35 were assigned for protons attached at C-3 and C-5 respectively. Proton at unsaturated C-7 appeared at δ 8.13 due to nitrogen and OH group being...

  7. Therapeutic Potential of Biologically Reduced Silver Nanoparticles from Actinomycete Cultures

    Silver nanoparticles are applied in nanomedicine from time immemorial and are still used as powerful antibiotic and anti-inflammatory agents. Antibiotics produced by actinomycetes are popular in almost all the therapeutic measures, and this study has proven that these microbes are also helpful in the biosynthesis of silver nanoparticles with good surface and size characteristics. Silver can be synthesized by various chemical methodologies, and most of them have turned to be toxic. This study has been successful in isolating the microbes from polluted environment, and subjecting them to the reduction of silver nanoparticles, characterizing the nanoparticles by UV spectrophotometry and transmission electron microscopy. The nanoparticles produced were tested for their antimicrobial property, and the zone of inhibition was greater than those produced by their chemically synthesized counterparts. Actinomycetes, helpful in bioremediating heavy metals, are useful for the production of metallic nanoparticles. The biosynthesized silver nanoparticles loaded with antibiotics prove to be better in killing the pathogens and have opened up new areas for developing nanobiotechnological research based on microbial applications.

  8. desde su cultura

    Luz Ángela Argote O.

    2008-01-01

    Full Text Available Para explorar las prácticas sobre protección y cuidado durante la transición del climaterio a la menopausia se llevó a cabo un estudio en mujeres colombianas afrodescendientes que viven en el Distrito de Aguablanca, Cali, Colombia, a fin de evaluar los factores socioculturales que influyen en su cuidado. Se siguió la etnoenfermería con el objeto de averiguar la perspectiva femenina étnica, e identificar las prácticas beneficiosas y de riesgo para su salud. La investigación tuvo como participantes claves a 17 mujeres. En la consolidación de la muestra se aplicaron los criterios de saturación de datos. En los resultados se evidenció la relación de las mujeres con su propio cuerpo, su defensa y fortificación desde la menarquia como fase preparatoria de sus funciones reproductivas, hasta cuando aparece la menopausia. De acuerdo con su cultura, si no tienen en cuenta estas prácticas tradicionales, sufrirán diversas dolencias en la etapa final de su periodo de procreación. Los cuidados sobresalientes se relacionan con el significado de la sangre, así como con el ejercicio de la sexualidad, la limpieza, la alimentación especial y el equilibrio que se debe guardar entre el calor y el frío. Las entrevistadas atribuyen a la menopausia cambios en su cuerpo, en sus diferencias emocionales de conducta y en las expectativas de la sexualidad. Para el presente estudio estos hallazgos se agruparon en tres temas principales: sentir los profundos cambios en la vida: el cuerpo; sentir los cambios en los estados de ánimo; y vivir la sexualidad.

  9. New Dimensions of Research on Actinomycetes: Quest for Next Generation Antibiotics.

    Jose, Polpass Arul; Jha, Bhavanath

    2016-01-01

    Starting with the discovery of streptomycin, the promise of natural products research on actinomycetes has been captivating researchers and offered an array of life-saving antibiotics. However, most of the actinomycetes have received a little attention of researchers beyond isolation and activity screening. Noticeable gaps in genomic information and associated biosynthetic potential of actinomycetes are mainly the reasons for this situation, which has led to a decline in the discovery rate of novel antibiotics. Recent insights gained from genome mining have revealed a massive existence of previously unrecognized biosynthetic potential in actinomycetes. Successive developments in next-generation sequencing, genome editing, analytical separation and high-resolution spectroscopic methods have reinvigorated interest on such actinomycetes and opened new avenues for the discovery of natural and natural-inspired antibiotics. This article describes the new dimensions that have driven the ongoing resurgence of research on actinomycetes with historical background since the commencement in 1940, for the attention of worldwide researchers. Coupled with increasing advancement in molecular and analytical tools and techniques, the discovery of next-generation antibiotics could be possible by revisiting the untapped potential of actinomycetes from different natural sources. PMID:27594853

  10. Diversity and Antagonistic Activity of Actinomycete Strains From Myristica Swamp Soils Against Human Pathogens

    Varghese Rlnoy

    2014-05-01

    Full Text Available Under the present investigation Actinomycetes were isolated from the soils of Myristica swamps of southern Western Ghats and the antagonistic activity against different human bacterial pathogens was evaluated. Results of the present study revealed that Actinomycetes population in the soils of Myristica swamp was spatially and seasonally varied. Actinomycetes load was varied from 24×104 to 71×103, from 129×103 to 40×103 and from 31×104 to 84×103 in post monsoon, monsoon and pre monsoon respectively. A total of 23 Actinomycetes strains belonging to six genera were isolated from swamp soils. Identification of the isolates showed that most of the isolates belonged to the genus Streptomyces (11, followed by Nocardia (6, Micromonospora (3, Pseudonocardia (1, Streptosporangium (1, and Nocardiopsis (1. Antagonistic studies revealed that 91.3% of Actinomycete isolates were active against one or more tested pathogens, of that 56.52% exhibited activity against Gram negative and 86.95% showed activity against Gram positive bacteria. 39.13% isolates were active against all the bacterial pathogens selected and its inhibition zone diameter was also high. 69.5% of Actinomycetes were exhibited antibacterial activity against Listeria followed by Bacillus cereus (65.21%, Staphylococcus (60.86%, Vibrio cholera (52.17%, Salmonella (52.17% and E. coli (39.13%. The results indicate that the Myristica swamp soils of Southern Western Ghats might be a remarkable reserve of Actinomycetes with potential antagonistic activity.

  11. Terrestrial actinomycetes from diverse locations of Uttarakhnad, India: Isolation and screening for their antibacterial activity.

    Vijay Kumar

    2013-09-01

    Full Text Available Uttarakhand region is less explored, but possess a great biodiversity. This diversity can be explored for isolation and characterization of new actinomycetes strains for seeking antimicrobial molecules. It can therefore be predicted that novel bioactive metabolite producing actinomycetes can be discovered to combat multidrug resistant bacterial pathogens.Variations in the viable count of actinomycetes were accessed in different altitudes. Actinomycetes were isolated, indentified and screened for their antibacterial activity.The highest viable counts of actinomycetes were recorded in valleys followed by mid hills and high hills. A total of 512 actinomycetes were isolated which were found to belong the 14 different genera of actinomycetes. Mainly the genus Streptomyces was dominant in all the soil samples. Out of 512 isolates recovered, 23.44% exhibited antibacterial activity against one or more tested bacterial pathogens. Of these 56.67% showed activity against Gram-positive bacteria, 26.67% against Gram-negative bacteria while 16.67% showed broad spectrum activity. Isolate DV1S and GR9a-5 showed highest antibacterial properties against several multi-drug resistant bacterial pathogens and were identified using polyphasic approach. DV1S and GR9a-5 were found to be most closely related with S. massasporeus NBRC 12796(T and Nocardia nova JCM 6044(T respectively.The results of this study strongly support the idea that the viable count of actinomycetes varied greatly with altitude. The actinomycetes species isolated from valleys, mid hills and high hills possess significant capacity to produce compounds which are active against several drug resistant bacterial pathogens.

  12. Studies on Actinomycetal Resources under Extreme Environments in the West of China

    Li, W.

    2005-12-01

    s: Actinomycetes play a quite important role in natural ecological system and they are also profile producers of antibiotics, antitumor agents, enzymes, enzyme inhibitors and immunomodifiers. which have been widely applied in industry, agriculture, forestry and pharmaceutical industry. In the past, the research work on actinomycetes was mainly concentrated on that of common habitats. Actinomycetes resources under extreme environments (including extreme high and low temperature, extreme high or low pH, high salt concentration etc.) have received comparatively little attention from microbiologists. Actinomycetes are regarded as one kind of sideline microorganisms and those under extreme environments are better materials for biological evolution and phylogenetic development in research. There are much more unknown species and much more worth researching for actinomycetes under extreme environments. There are many extreme environmental resources in the west of China. For example, wide range snow-mountains, basified soil and lakes, widely distributed acid and alkaline hot-springs in Yunnan provinces; more than 73.3 million hektares basified soil and salt lakes in Xinjiang Province and many unusual environments in Qinghai Province and other western Provinces. They were mostly precious natural resources and were destroyed, relatively fewer can provided us with unique conditions for study on actinomycetal resources under extreme environments. In recent years, our main work was focusing on study of extremophilic actinomycetal resources in the west of China by using conventional cultivation-methods and culture-independent methods (PCR-clone and DGGE/TGGE, etc), Results showed that large amount of unknown microbial resources (including actinomycetal resources) existed in natural extreme environments. Additionally, lots of new taxa were isolated and characterized using a polyphasic approach. Further, we got some new compounds with different bioactivities from these

  13. Mundos aislados: segregación urbana y desigualdad en la ciudad de México

    GONZALO A SARAVÍ

    2008-12-01

    Full Text Available De qué nos habla la segregación urbana en México y cuáles son sus implicaciones en este contexto en particular, son las dos preguntas centrales que guían el análisis desarrollado en este artículo. El argumento que responde a estas dos preguntas se estructura en torno a la hipótesis de una "coexistencia de mundos aislados". Para sustentar dicha hipótesis, el autor analiza teórica y empíricamente la relación entre las dimensiones objetiva y simbólica de la segregación urbana. El análisis se basa en datos secundarios y en información obtenida a partir de entrevistas semi-estructuradas con jóvenes residentes en colonias populares y estigmatizadas de la Ciudad de México.What does urban segregation mean in México and what are its implications for this particular context? This article poses these two questions. Our response is based on the hypothesis of the "co-existence of isolated worlds." In order to support and develop this hypothesis, the author analyses the relationship between the objective and symbolic dimensions of urban segregation. The analysis is based on secondary data and semi-structured interviews with young people from poor and stigmatized neighborhoods in México City.

  14. New Benzoxazine Secondary Metabolites from an Arctic Actinomycete

    Kyuho Moon

    2014-04-01

    Full Text Available Two new secondary metabolites, arcticoside (1 and C-1027 chromophore-V (2, were isolated along with C-1027 chromophore-III and fijiolides A and B (3–5 from a culture of an Arctic marine actinomycete Streptomyces strain. The chemical structures of 1 and 2 were elucidated through NMR, mass, UV, and IR spectroscopy. The hexose moieties in 1 were determined to be d-glucose from a combination of acid hydrolysis, derivatization, and gas chromatographic analyses. Arcticoside (1 and C-1027 chromophore-V (2, which have a benzoxazine ring, inhibited Candida albicans isocitrate lyase. Chromophore-V (2 exhibited significant cytotoxicity against breast carcinoma MDA-MB231 cells and colorectal carcinoma cells (line HCT-116, with IC50 values of 0.9 and 2.7 μM, respectively.

  15. Antiviral property of marine actinomycetes against white spot syndrome virus in penaeid shrimps

    Kumar, S.S.; Philip, R.; Achuthankutty, C.T.

    Aquaculture farms, particularly in Southeast Asia are facing severe crisis due to increasing incidences of White Spot Syndrome Virus (WSSV). Actinomycetes have provided many important bioactive compounds of high prophylactic and therapeutic value...

  16. Psychrotolerant actinomycetes of plants and organic horizons in tundra and taiga soils

    Dubrova, M. S.; Zenova, G. M.; Yakushev, A. V.; Manucharova, N. A.; Makarova, E. P.; Zvyagintsev, D. G.; Chernov, I. Yu.

    2013-08-01

    It has been revealed that in organic horizons and plants of the tundra and taiga ecosystems under low temperatures, actinomycetal complexes form. The population density of psychrotolerant actinomycetes in organic horizons and plants reaches tens and hundreds of thousands CFU/g of substrate or soil, and decreases in the sequence litters > plants > soils > undecomposed plant remains > moss growths. The mycelium length of psychrotolerant actinomycetes reaches 220 m/g of substrate. Application of the FISH method has demonstrated that metabolically active psychrotolerant bacteria of the phylum Actinobacteria constitute 30% of all metabolically active psychrotolerant representatives of the Bacterià domain of the prokaryotic microbial community of soils and plants. Psychrotolerant actinomycetes in tundra and taiga ecosystems possess antimicrobial properties.

  17. Specificity of actinomycetal complexes in urbanozems of the city of Kirov

    Shirokikh, I. G.; Ashikhmina, T. Ya.; Shirokikh, A. A.

    2011-02-01

    The number and composition of the actinomycetal population was studied in urbanozems in the city of Kirov. It was shown that the total population of actinomycetes was an order of magnitude lower than that in the background territories, and the generic structure of the actinomycetal complex and the species composition of the streptomycetes were transformed under the influence of the urbanization factors. The obtained data were compared with the concentrations of the mobile forms of Pb, Zn, Cu, Fe, and Mn in different ecotopes (industrial, traffic, and recreation zones). The increase of the relative portion of micromonosporic actinomycetes in comparison with the background (reference) soils was observed in the complexes of the industrial and transport ecotopes mostly contaminated with heavy metals. It was found that the antibiotic potential of the streptomycetes in the contaminated soils was lower than in the soils of the background territories.

  18. Marine actinomycetes as an emerging resource for the drug development pipelines.

    Zotchev, Sergey B

    2012-04-30

    Many representatives of the order Actinomycetales are prolific producers of thousands of biologically active secondary metabolites. Actinomycetes from terrestrial sources have been studied and screened since the 1950s, yielding many important anti-infective and anti-cancer drugs. However, frequent re-discovery of the same compounds in terrestrial actinomycetes have made them less attractive for screening programs in the recent years. At the same time, actinomycetes isolated from the marine environment currently receive considerable attention due to the structural diversity and unique biological activities of their secondary metabolites. This review highlights achievements and challenges in the isolation of marine actinomycetes, some examples of bioactive metabolites identified by conventional screening, and presents new developments in the field of genome mining and heterologous expression of biosynthetic gene clusters leading to the discovery of novel compounds. PMID:21683100

  19. Isolation and identification of actinomycetes from a compost-amended soil with potential as biocontrol agents.

    Cuesta, Gonzalo; García-de-la-Fuente, Rosana; Abad, Manuel; Fornes, Fernando

    2012-03-01

    The search for new biocontrol strategies to inhibit the growth of phytopathogenic microorganisms has become widely widespread due to environmental concerns. Among actinomycetes, Streptomyces species have been extensively studied since they have been recognized as important sources of antibiotics. Actinomycete strains were isolated from a calcareous soil, 2 two-phase olive mill waste ('alperujo') composts, and the compost-amended soil by using selective media, and they were then co-cultured with 5 phytopathogenic fungi and 1 bacterium to perform an in vitro antagonism assay. Forty-nine actinomycete strains were isolated, 12 of them showing a great antagonistic activity towards the phytopathogenic microorganisms tested. Isolated strains were identified by 16S rDNA sequence analysis and phenotypic procedures. Eleven isolates concerned the genus Streptomyces and 1 actinomycete with chitinolytic activity belonged to the genus Lechevalieria. PMID:21190787

  20. Actinomycetes as the causative organism of osteomyelitis in sickle cell disease

    The case of a 17-year-old girl with sickle cell anaemia who presented with extensive osteomyelitis due to actinomycetes is reported. Osteomyelitis in the long bones due to actinomycosis is extremely rare. A review of the literature reveals only six cases in which actinomycetes have been isolated from lesions affecting a long bone. The occurence of this condition in sickle cell haemoglobinopathy has not been previously reported. (orig.)

  1. Isolation of endophytic actinomycetes from roots and leaves of maize (Zea mays L.)

    Araújo, Janete M.; da Silva, Adilson C.; João L. Azevedo

    2000-01-01

    Actinomycetes were isolated from surface sterilized leaves and roots of maize. A total of 53 isolates were obtained, 31 of them from leaves and 22 from roots. The genus Microbispora was the most frequently found followed by the genera Streptomyces and Streptosporangium. From the isolated actinomycetes, 43.4% showed antimicrobial activity against one or more tested bacteria and yeast.Microrganismos endofíticos são frequentemente encontrados no interior de plantas cultivadas. Embora vários tipo...

  2. Actinomycetes from the South China Sea sponges: isolation, diversity, and potential for aromatic polyketides discovery

    Sun, Wei; Zhang, Fengli; He, Liming; Karthik, Loganathan; Li, Zhiyong

    2015-01-01

    Marine sponges often harbor dense and diverse microbial communities including actinobacteria. To date no comprehensive investigation has been performed on the culturable diversity of the actinomycetes associated with South China Sea sponges. Structurally novel aromatic polyketides were recently discovered from marine sponge-derived Streptomyces and Saccharopolyspora strains, suggesting that sponge-associated actinomycetes can serve as a new source of aromatic polyketides. In this study, a tot...

  3. Population densities and genetic diversity of actinomycetes associated to the rhizosphere of Theobroma cacao

    Tâmara R. Barreto; da Silva, Augusto C.M.; Soares, Ana Cristina F.; De Souza, Jorge T.

    2008-01-01

    In spite of the acknowledged importance of growth-promoting bacteria, only a reduced number of studies were conducted with these microorganisms on Theobroma cacao. The objectives of this work were to study the population densities and genetic diversity of actinomycetes associated with the rhizosphere of cacao as a first step in their application in plant growth promotion and biological control. The populations densities of actinomycetes in soil and cacao roots were similar, with mean values o...

  4. Isolation and in vitro selection of actinomycetes strains as potential probiotics for aquaculture

    Milagro García Bernal; Ángel Isidro Campa-Córdova; Pedro Enrique Saucedo; Marlen Casanova González; Ricardo Medina Marrero; José Manuel Mazón-Suástegui

    2015-01-01

    Aim: This study was designed to describe a series of in vitro tests that may aid the discovery of probiotic strains from actinomycetes. Materials and Methods: Actinomycetes were isolated from marine sediments using four different isolation media, followed by antimicrobial activity and toxicity assessment by the agar diffusion method and the hemolysis of human blood cells, respectively. Extracellular enzymatic production was monitored by the hydrolysis of proteins, lipids and carbohydrates....

  5. Identification of the Entner-Doudoroff pathway in an antibiotic-producing actinomycete species

    Gunnarsson, Nina; Mortensen, Uffe Hasbro; Sosio, M.; Nielsen, Jens

    2004-01-01

    the primary metabolic pathways of the poorly characterized antibiotic-producing actinomycete Nonomuraea sp. ATCC 39727. Surprisingly, it was found that Nonomuraea sp. ATCC 39272 predominantly metabolizes glucose via the Entner-Doudoroff (ED) pathway. This represents the first time that the ED pathway...... has been recognized as the main catabolic pathway in an actinomycete. The Nonomuraea genes encoding the key enzymes of the ED pathway were subsequently identified, sequenced and functionally described....

  6. Artificial Chromosomes to Explore and to Exploit Biosynthetic Capabilities of Actinomycetes

    Rosa Alduina; Giuseppe Gallo

    2012-01-01

    Actinomycetes are an important source of biologically active compounds, like antibiotics, antitumor agents, and immunosuppressors. Genome sequencing is revealing that this class of microorganisms has larger genomes relative to other bacteria and uses a considerable fraction of its coding capacity (5–10%) for the production of mostly cryptic secondary metabolites. To access actinomycetes biosynthetic capabilities or to improve the pharmacokinetic properties and production yields of these chemi...

  7. Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes

    Xiang Xiao; Jun Xu; Min-Juan Xu; Shu-Jie Xie; Li Xu; Guang-Hui Ma; Xiao-Min Tang; Jing Xiao; Xue-Gong Li

    2013-01-01

    Mangrove-derived actinomycetes are promising sources of bioactive natural products. In this study, using homologous screening of the biosynthetic genes and anti-microorganism/tumor assaying, 163 strains of actinomycetes isolated from mangrove sediments were investigated for their potential to produce halogenated metabolites. The FADH2-dependent halogenase genes, identified in PCR-screening, were clustered in distinct clades in the phylogenetic analysis. The coexistence of either polyketide sy...

  8. Exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea.

    Sreevidya, M; Gopalakrishnan, S; Kudapa, H; Varshney, R K

    2016-01-01

    The main objective of the present study was to isolate and characterize actinomycetes for their plant growth-promotion in chickpea. A total of 89 actinomycetes were screened for their antagonism against fungal pathogens of chickpea by dual culture and metabolite production assays. Four most promising actinomycetes were evaluated for their physiological and plant growth-promotion properties under in vitro and in vivo conditions. All the isolates exhibited good growth at temperatures from 20°C to 40°C, pH range of 7-11 and NaCl concentrations up to 8%. These were also found highly tolerant to Bavistin, slightly tolerant to Thiram and Captan (except VAI-7 and VAI-40) but susceptible to Benlate and Ridomil at field application levels and were found to produce siderophore, cellulase, lipase, protease, chitinase (except VAI-40), hydrocyanic acid (except VAI-7 and VAI-40), indole acetic acid and β-1,3-glucanase. When the four actinomycetes were evaluated for their plant growth-promotion properties under field conditions on chickpea, all exhibited increase in nodule number, shoot weight and yield. The actinomycetes treated plots enhanced total N, available P and organic C over the un-inoculated control. The scanning electron microscope studies exhibited extensive colonization by actinomycetes on the root surface of chickpea. The expression profiles for indole acetic acid, siderophore and β-1,3-glucanase genes exhibited up-regulation for all three traits and in all four isolates. The actinomycetes were identified as Streptomyces but different species in the 16S rDNA analysis. It was concluded that the selected actinomycetes have good plant growth-promotion and biocontrol potentials on chickpea. PMID:26887230

  9. Isolation and characterization of actinomycete antagonists of a fungal root pathogen.

    Crawford, D L; Lynch, J M; Whipps, J M; Ousley, M A

    1993-11-01

    By use of selective media, 267 actinomycete strains were isolated from four rhizosphere-associated and four non-rhizosphere-associated British soils. Organic media with low nutrient concentrations were found to be best for isolating diverse actinomycetes while avoiding contamination and overgrowth of isolation media by eubacteria and fungi. While all isolates grew well at pHs 6.5 to 8.0, a few were unable to grow at pH 6.0 and a significant number failed to grow at pH 5.5. Eighty-two selected isolates were screened for in vitro antagonism towards Pythium ultimum by use of a Difco cornmeal agar assay procedure. Five isolates were very strong antagonists of the fungus, four were strong antagonists, and ten others were weakly antagonistic. The remaining isolates showed no antagonism by this assay. Additional studies showed that several of the P. ultimum antagonists also strongly inhibited growth of other root-pathogenic fungi. Twelve isolates showing antifungal activity in the in vitro assay were also tested for their effects on the germination and short-term growth of lettuce plants in glasshouse pot studies in the absence of pathogens. None of the actinomycetes prevented seed germination, although half of the isolates retarded seed germination and outgrowth of the plants by 1 to 3 days. During 18-day growth experiments, biomass yields of some actinomycete-inoculated plants were reduced in comparison with untreated control plants, although all plants appeared healthy and well rooted. None of the actinomycetes significantly enhanced plant growth over these short-term experiments. For some, but not all, actinomycetes, some correlations between delayed seed germination and reduced 18-day plant biomass yields were seen. For others, plant biomass yields were not reduced despite an actinomycete-associated delay in seed germination and plant outgrowth. Preliminary glasshouse experiments indicated that some of the actinomycetes protect germinating lettuce seeds against

  10. Occurrence of Rhodococcus coprophilus and associated actinomycetes in feces, sewage, and freshwater.

    Mara, D. D.; Oragui, J I

    1981-01-01

    Freshwater, sewage, and fecal samples from various sources were examined for Rhodococcus coprophilus, associated actinomycetes, Escherichia coli, and fecal streptococci. Rhodococcus coprophilus was isolated consistently from feces of farm animals, poultry reared in proximity to farm animals, freshwater, and wastewater polluted with animal fecal wastes. It was not isolated from samples of human feces. The ratio of R. coprophilus total actinomycetes was higher in feces from cattle, sheep, ducks...

  11. Bioperspective of actinomycetes isolates from coastal soils: A new source of antimicrobial producers

    Rattanaporn Srivibool; Morakot Sukchotiratana

    2006-01-01

    Forty five soil samples were collected from four coastal islands on the east coast of Thailand: Chang, Hwai, Lao-yanai in Trat Province and Pai Islands in Chonburi Province. On 3 isolating media, Actinomycetes Isolation Agar, Starch Casein Agar and Glucose Asparagine Agar, 495 isolates of actinomycetes were found. Preliminary test to search for antimicrobial activity was done with Bacillus subtilis TISTR 008, Staphylococcus aureus TISTR 885, Staphylococus aureus TISTR 517 (ATCC 25923), Microc...

  12. NUEVOS AISLADOS DE Gluconacetobacter diazotrophicus EN CULTIVOS DE IMPORTANCIA ECONÓMICA PARA CUBA

    B. Dibut

    2005-01-01

    Full Text Available En los últimos años, un llamado importante para los microbiólogos del suelo ha sido el estudio de microorganismos endófitos que se asocian con plantas superiores, con el consiguiente beneficio sobre los cultivos. En este trabajo, se ofrecen los resultados sobre el aislamiento y la distribución de Gluconacetobacter diazotrophicus en cultivos de importancia económica para Cuba. La bacteria se aisló a partir de filtrados y secciones de diferentes órganos del vegetal dispuestos sobre medios de cultivo LGI-P, donde se comprobó el crecimiento característico a las 96 h de incubación a 320C de temperatura, obteniéndose finalmente 22 aislados a partir de diez especies cultivables. Experimentos de dinámica poblacional desarrollados en condiciones de invernadero mediante diseño completamente aleatorizado permitieron cuantificar el microorganismo en hojas, tallos y raíces de maíz, boniato, yuca, malanga y caña de azúcar, con poblaciones que oscilan entre 1.8 x 102-2.3 x 107 células por gramo de tejido fresco. Las poblaciones celulares más altas se detectaron en las hojas, seguido de los tallos y por último las raíces o tubérculos. La respuesta favorable de ocho cultivos a la inoculación de la bacteria, esta vez crecida en medio de cultivo SG e incubada a 320C durante 72 h, permite plantear la potencialidad que este microorganismo presenta como biofertilizante. Se informa por primera vez la presencia de la Gluconacetobacter diazotrophicus en cinco especies de plantas pertenecientes cada una a diferentes familias botánicas.

  13. Enrichment Method for the Isolation of Bioactive Actinomycetes From Mangrove Sediments of Andaman Islands, India

    Baskaran, R.

    2011-01-01

    Full Text Available Various pre-treatment methods and three different media were employed for the isolation of bioactive actinomycetes from mangrove sediments of Andaman and Nicobar Islands, India. Sediments from four different sites of mangrove forest were collected and pre-treated by dry heat method, and the media were supplemented with cycloheximide 80 µg/mL and nalidixic acid 75 µg/mL. The mean actinomycetes population density in sediment samples were recorded as 22 CFU-10^-6/gm in KUA medium followed by 12 CFU-10^-6/gm in AIA medium and 8 CFU-10^-6/gm in SCA medium. A total of 42 actinomycetes were isolated, and all the isolates were evaluated for their antibacterial activity against pathogenic bacteria on two different media. Among 42 isolates tested, 22 species were found to be antibacterial metabolite producer against test bacteria namely, Staphylococcus aureus, Bacillus subtilis, Salmonella typhi and Klebsiella pneumoniae. Particularly, the actinomycete strains such as A101, A102, A107, A116, A121, A125, A130, F101, F102, F104, F106, De101 and De102 significantly inhibited the growth of all bacteria which were tested. Of these strains, A107 was identified as Streptomyces spp. This strain had the maximum activity against all used pathogens on both medium. Hence, the isolation, characterization and studies of secondary metabolites of actinomycetes from mangrove sediments in Andaman and Nicobar Island could be a pathway for discovery of antibiotics from marine actinomycetes.

  14. Evaluación in vitro de la actividad inhibitoria de extractos vegetales sobre aislados de Colletotrichum spp

    Rojas Sierra2 Johanna

    2011-06-01

    Full Text Available Se evaluó la actividad inhibitoria in vitro de extractos de hojas de Melissa officinalis, Origanum vulgare,Jatropha gossypilia, Eucalyptus sp., Melia azederach y Mascagnia concinna sobre aislados de hongo delgénero Colletotrichum, causante de la enfermedad antracnosis en el cultivo del ñame (Dioscorea alata, D.rotundata en el departamento de Sucre, Colombia. Para la obtención de extractos etanólicos de hojasde M. officinalis y O. vulgare, se utilizó el método de percolación y para las otras especies vegetales, elmétodo de Soxhlet. Una vez preparados los extractos de hojas, se evaluó su actividad inhibitoria sobreseis aislados de hongo del género Colletotrichum (BVC 279a, BVC 279b, BVC 853, BVC 040, BVC 342 yBVC 507. Para la prueba inhibitoria se utilizó el método de siembra directa sobre la superficie del mediopapa-dextrosa-agar. Sobre las diferentes cepas se adicionaron 250 μl de cada extracto por separado. Seutilizó un control positivo con nistatina (4 mg/ml y un testigo absoluto sin ningún tipo de tratamiento.La prueba se evaluó midiendo el crecimiento radial de cada cepa con los diferentes tratamientos a 96y 168 h. Se utilizó un diseño de bloques completamente al azar con tres repeticiones, para probardiferencias significativas entre tratamientos, cepas (aislados y tiempo de inhibición. Los resultadosexpresados en porcentaje de inhibición, mostraron una alta actividad de los extractos obtenidos de lashojas de las plantas de M. azederach y M. concinna, sobre los hongos evaluados y su acción fue similaral control positivo

  15. Evaluación in vitro de la actividad inhibitoria de extractos vegetales sobre aislados de Colletotrichum spp

    Alexander Pérez Cordero

    2011-04-01

    Full Text Available Se evaluó la actividad inhibitoria in vitro de extractos de hojas de Melissa officinalis, Origanum vulgare, Jatropha gossypilia, Eucalyptus sp., Melia azederach y Mascagnia concinna sobre aislados de hongo del género Colletotrichum, causante de la enfermedad antracnosis en el cultivo del ñame (Dioscorea alata, D. rotundata en el departamento de Sucre, Colombia. Para la obtención de extractos etanólicos de hojas de M. officinalis y O. vulgare, se utilizó el método de percolación y para las otras especies vegetales, el método de Soxhlet. Una vez preparados los extractos de hojas, se evaluó su actividad inhibitoria sobre seis aislados de hongo del género Colletotrichum (BVC 279a, BVC 279b, BVC 853, BVC 040, BVC 342 y BVC 507. Para la prueba inhibitoria se utilizó el método de siembra directa sobre la superficie del medio papa-dextrosa-agar. Sobre las diferentes cepas se adicionaron 250 µl de cada extracto por separado. Se utilizó un control positivo con nistatina (4 mg/ml y un testigo absoluto sin ningún tipo de tratamiento. La prueba se evaluó midiendo el crecimiento radial de cada cepa con los diferentes tratamientos a 96 y 168 h. Se utilizó un diseño de bloques completamente al azar con tres repeticiones, para probar diferencias significativas entre tratamientos, cepas (aislados y tiempo de inhibición. Los resultados expresados en porcentaje de inhibición, mostraron una alta actividad de los extractos obtenidos de las hojas de las plantas de M. azederach y M. concinna, sobre los hongos evaluados y su acción fue similar al control positivo.

  16. Influence of moisture on the vital activity of actinomycetes in a cultivated low-moor peat soil

    Zenova, G. M.; Gryadunova, A. A.; Doroshenko, E. A.; Likhacheva, A. A.; Sudnitsyn, I. I.; Pochatkova, T. N.; Zvyagintsev, D. G.

    2007-05-01

    It was found that the actinomycetal complex of a cultivated low-moor peat soil is characterized by a high population density and diversity of actinomycetes; representatives of eleven genera were isolated from this soil: Streptomyces, Micromonospora, Actinomadura, Saccharopolyspora, Microbispora, Microtetraspora, Streptosporangium, Nocardioides, Saccharomonospora, Kibdelosporangium, and Thermomonospora. Some genera were isolated from the soil under all the studied levels of soil moisture. The so-called rare (rarely occurring) genera (Saccharomonospora, Kibdelosporangium, and Thermomonospora) were isolated upon the low level of soil moisture, which ensured an absence of competition from the more abundant actinomycetes. Spores of all the studied actinomycetes could germinate under the low moisture level (a w = 0.67). The level of moisture a w = 0.98 was found to be optimal for the development of the actinomycetes. The complete cycle of the development of all the actinomycetes up to spore formation occurring was only observed under the high moisture level (a w = 0.98).

  17. Estudio anatomopatológico de aislados de Leptospira spp., provenientes de Nicaragua en Mesocricetus auratus como biomodelo

    Willian Jirón T.; Niurka Batista S.; Daniel Arencibia A.; Luis Rosario F.; Juan Infante B.

    2015-01-01

    RESUMENObjetivo. El objetivo de este trabajo fue caracterizar en el biomodelo Mesocricetus auratus la sintomatología y lesiones anatomopatológicas que provocan 5 aislados clínicos de Leptospira spp., provenientes de Nicaragua. Materiales y métodos. Con este fin se inocularon 50 hámster por vía i.p con 1mL del cultivo de cada una de las cepas en fase exponencial teniendo una concentración celular de 7.5 x 106 leptospira/mL (10 animales por cepa), evaluándose signos de la enfermedad, mortalidad...

  18. Actividad antinociceptiva, anti-inflamatoria y relajante muscular de (+)-curcufenol aislado de la esponja marina didiscus oxeata

    Salama, Ahmed; Toscano, Mariela; Del Valle, Martha; Vargas, Erika

    2008-01-01

    Mediante el método de edema plantar inducido por carragenina en la pata de la rata, (+)-curcufenol aislado de la esponja marina Didiscus oxeata mostró una actividad antiinflamatoria moderada, en las dosis de 100, 150 y 200 mg/kg administradas por vía oral, mientras que la actividad antinociceptiva fue confirmada por el método de contorsiones inducidas por ácido acético en ratones mostrando un efecto analgésico muy alto, en las dosis de 50, 100, 150 y 200 mg/kg administradas por vía oral, en c...

  19. Neuroprotecci??n mediada por diterpenos aislados de Sideritis spp. frente al estr??s oxidativo en astrocitos

    Gonz??lez-Burgos, E.; Palomino, O.M.; Carretero Accame, M.E.; G??mez-Serranillos, M.P.

    2010-01-01

    La presente investigaci??n aborda el estudio de las propiedades neuroprotectoras, en base a la capacidad antioxidante, de los diterpenos andalusol, conchitriol y sidol aislados de diferentes especies del g??nero Sideritis. La actividad protectora de estos compuestos fue evaluada en un modelo de estr??s oxidativo inducido por el per??xido de hidr??geno sobre la l??nea celular U373 MG de astrocitoma humano. Los resultados mostraron que un pretratamiento durante 24 horas con los dite...

  20. Variabilidad genética de Colletotrichum spp aislado de frutos tropicales mediante el uso de marcadores moleculares RAPD.

    Cruz Hernández, Miguel Angel

    2013-01-01

    Colletotrichum gloeosporioides presenta alta variabilidad morfológica, patogénica y genética, lo cual ha sido difícil de evaluar usando los métodos tradicionales. En los últimos años, las técnicas moleculares han facilitado la separación y caracterización genética. El presente trabajo se divide en dos fases, en la primera se realizaron los aislados, la identificación morfológica y se realizaron pruebas de efectividad bilógica in vivo e in vitro de imazalil para el control de la antracnosis en...

  1. Enumerating actinomycetes in compost bioaerosols at source—Use of soil compost agar to address plate ‘mask

    Taha, M. P. M.; Drew, Gillian H; Tamer Vestlund, Asli; Aldred, David; Longhurst, Philip J.; Pollard, Simon J. T.

    2007-01-01

    Actinomycetes are the dominant bacteria isolated from bioaerosols sampled at composting facilities. Here, a novel method for the isolation of actinomycetes is reported, overcoming masking of conventional agar plates, as well as reducing analysis time and costs. Repeatable and reliable actinomycetes growth was best achieved using a soil compost media at an incubation temperature of 44 °C and 7 days’ incubation. The results are of particular value to waste management operators...

  2. Transformation of 2,4,6-trinitrotoluene (TNT) by actinomycetes isolated from TNT-contaminated and uncontaminated environments.

    Pasti-Grigsby, M B; Lewis, T A; Crawford, D. L.; Crawford, R L

    1996-01-01

    Actinomycete strains isolated from 2,4,6-trinitrotoluene (TNT)-contaminated and uncontaminated environments were compared for TNT tolerance and abilities to transform TNT. Regardless of previous TNT exposure history, no significant differences in TNT tolerance were seen among strains. Selected strains did not significantly mineralize [14C]TNT. The actinomycetes did, however, transform TNT into reduced intermediates. The data indicate that, in actinomycete-rich aerobic environments like compos...

  3. Functional Gene-Guided Discovery of Type II Polyketides from Culturable Actinomycetes Associated with Soft Coral Scleronephthya sp

    Sun, Wei; Peng, Chongsheng; Zhao, Yunyu; Li, Zhiyong

    2012-01-01

    Compared with the actinomycetes in stone corals, the phylogenetic diversity of soft coral-associated culturable actinomycetes is essentially unexplored. Meanwhile, the knowledge of the natural products from coral-associated actinomycetes is very limited. In this study, thirty-two strains were isolated from the tissue of the soft coral Scleronephthya sp. in the East China Sea, which were grouped into eight genera by 16S rDNA phylogenetic analysis: Micromonospora, Gordonia, Mycobacterium, Nocar...

  4. Diversity of Actinomycetes at Several Forest Types in Wanagama I Yogyakarta and Their Potency as a Producer of Antifungal Compound

    Reni Nurjasmi; Jaka Widada; Ngadiman N

    2015-01-01

    Actinomycetes are bacterial groups that produce many secondary metabolites, which different biological activities, such as antifungi, antibacteria, antivirus, antitumor, etc. Actinomycetes are widely distributed in soil and their diversity is influenced by type of forest. The aim of this study is to investigate diversity of actinomycetes in several forest types of Wanagama I forest in Yogyakarta and their potency as a producer of antifungal compound. Soil samples under the forest of Tectona g...

  5. Detección y caracterización de aislados de "escherichia coli" de origen clínico y fecal en gallinas ponedoras

    Gibert Perelló, Magdalena

    2009-01-01

    El objetivo de esta tesis ha sido realizar la caracterización de aislados de E. coli clínicos (responsables de cuadros de colibacilosis en gallinas ponedoras) y fecales. Tras llevar a cabo el aislamiento e identificación bioquímica de los aislados, se ha procedido a la determinación del serogrupo, patotipo (detección de factores de virulencia por PCR), sensibilidad a antimicrobianos y pulsotipo (mediante la técnica de Pulsed Field-Gel Electrophoresis, PFGE). Además, se ha realizado un estudio...

  6. Nuevo alcaloide oxoaporfínico y otros constituyentes químicos aislados de Pleurothyrium cinereum (Lauraceae.

    Ericsson Coy

    2009-04-01

    Full Text Available Del extracto etanólico de las hojas de Pleurothyrium cinereum (Lauraceae fue aislado el nuevo alcaloide oxoaporfínico oxiprenilado 1,2-metilendioxi-9,10-dimetoxi-3-isopreniloxi-7H-dibenzo[de,g]quinolin-7-ona (Pleurotirina 1, el cual fue purificado por  métodos cromatográficos y cuya elucidación estructural se realizó mediante técnicas espectroscópicas (RMN-1H, RMN-13C, RMN-2D y EM. Junto al alcaloide 1 fueron aislados los compuestos thalicminina 2, ácido ent-kaurenóico 3, alloxantoxiletina 4, xantiletina 5, dihydroflavokawina B 6, 3’-metoxi-3,4-metilendioxi-4’,7-epoxi-nor-8.5’-neolignan-7.8’-dieno 7 y friedelina 8, los cuales se reportan por primera vez para la especie y para el género.

  7. Caracterización bioquímica y molecular de aislados de Klebsiella pneumoniae resistente a antimicrobianos

    Laura Margarita Castañeda

    2000-02-01

    diferentes aislados bacterianos resistentes a antibióticos, son insuficientes para discriminar un posible brote de infección nosocomial, siendo necesaria la utilización de técnicas basadas en el estudio genotípico, que logren establecer diferencias entre los aislados.

    El análisis del perfil de plásmidos es de mucha aplicabilidad, ya que permite una evaluación epidemiológica útil para determinar la diferencia o la semejanza entre los aislados sometidos a estudio. Sin embargo, no es suficiente para determinar el origen clonal de un brote y se requiere un método que permita una caracterización mas precisa de las cepas. Para este fin, se utiliza el análisis del DNA genómico, cortado con endonucleasas de restricción con baja frecuencia de corte, por medio de la electroforesis de campo pulsado (PFGE, que ha mostrado ser una buena herramienta epidemiológica, debido a su alta reproducibilidad y a su poder discriminatorio para determinar el origen clonal en varias especies bacterianas incluyendo Klebsiella pneunoniae, Este proyecto pretende determinar y caracterizar el comportamiento genético de esta resistencia en las cepas de Klebsiella pneumoniae circulantes en nuestro medio, lo que permitirá plantear estrategias epidemiológicas en el manejo y prevención de las diferentes infecciones debidas a este microorganismo.

    Objetivo general: Caracterizar bioquímica y molecularmente aislados de Klebsiella pneumoniae resistentes a antimicrobianos.

    Objetivos específicos: 1. Biotipificar los aislados de Klebsiella pneumoniae. 2. Determinar la susceptibilidad de los aislados, incluyendo la detección de la producci

  8. Characterization of a chitinase from the cellulolytic actinomycete Thermobifida fusca.

    Gaber, Yasser; Mekasha, Sophanit; Vaaje-Kolstad, Gustav; Eijsink, Vincent G H; Fraaije, Marco W

    2016-09-01

    Thermobifida fusca is a well-known cellulose-degrading actinomycete, which produces various glycoside hydrolases for this purpose. However, despite the presence of putative chitinase genes in its genome, T. fusca has not been reported to grow on chitin as sole carbon source. In this study, a gene encoding a putative membrane-anchored GH18 chitinase (Tfu0868) from T. fusca has been cloned and overexpressed in Escherichia coli. The protein was produced as SUMO fusion protein and, upon removal of the SUMO domain, soluble pure TfChi18A was obtained with yields typically amounting to 150mg per litre of culture. The enzyme was found to be relatively thermostable (apparent Tm=57.5°C) but not particularly thermoactive, the optimum temperature being 40-45°C. TfChi18A bound to α- and β-chitin and degraded both these substrates. Interestingly, activity towards colloidal chitin was minimal and in this case, substrate inhibition was observed. TfChi18A also cleaved soluble chito-oligosaccharides and showed a clear preference for substrates having five sugars or more. While these results show that TfChi18A is a catalytically competent GH18 chitinase, the observed catalytic rates were low compared to those of well-studied GH18 chitinases. This suggests that TfChi18A is not a true chitinase and not likely to endow T. fusca with the ability to grow on chitin. PMID:27108953

  9. Actinomycetes from red sea sponges: Sources for chemical and phylogenetic diversity

    Abdelmohsen, Usama Ramadan

    2014-05-12

    The diversity of actinomycetes associated with marine sponges collected off Fsar Reef (Saudi Arabia) was investigated in the present study. Forty-seven actinomycetes were cultivated and phylogenetically identified based on 16S rRNA gene sequencing and were assigned to 10 different actinomycete genera. Eight putatively novel species belonging to genera Kocuria, Mycobacterium, Nocardia, and Rhodococcus were identified based on sequence similarity values below 98.2% to other 16S rRNA gene sequences available in the NCBI database. PCR-based screening for biosynthetic genes including type I and type II polyketide synthases (PKS-I, PKS-II) as well as nonribosomal peptide synthetases (NRPS) showed that 20 actinomycete isolates encoded each at least one type of biosynthetic gene. The organic extracts of nine isolates displayed bioactivity against at least one of the test pathogens, which were Gram-positive and Gram-negative bacteria, fungi, human parasites, as well as in a West Nile Virus protease enzymatic assay. These results emphasize that marine sponges are a prolific resource for novel bioactive actinomycetes with potential for drug discovery. 2014 by the authors; licensee MDPI.

  10. Actinomycetes from Red Sea Sponges: Sources for Chemical and Phylogenetic Diversity

    Usama Ramadan Abdelmohsen

    2014-05-01

    Full Text Available The diversity of actinomycetes associated with marine sponges collected off Fsar Reef (Saudi Arabia was investigated in the present study. Forty-seven actinomycetes were cultivated and phylogenetically identified based on 16S rRNA gene sequencing and were assigned to 10 different actinomycete genera. Eight putatively novel species belonging to genera Kocuria, Mycobacterium, Nocardia, and Rhodococcus were identified based on sequence similarity values below 98.2% to other 16S rRNA gene sequences available in the NCBI database. PCR-based screening for biosynthetic genes including type I and type II polyketide synthases (PKS-I, PKS-II as well as nonribosomal peptide synthetases (NRPS showed that 20 actinomycete isolates encoded each at least one type of biosynthetic gene. The organic extracts of nine isolates displayed bioactivity against at least one of the test pathogens, which were Gram-positive and Gram-negative bacteria, fungi, human parasites, as well as in a West Nile Virus protease enzymatic assay. These results emphasize that marine sponges are a prolific resource for novel bioactive actinomycetes with potential for drug discovery.

  11. Isolation of actinomycetes from mangrove and estuarine sediments of Cochin and screening for antimicrobial activity

    Emilda Rosmine

    2016-01-01

    Objective:To isolate and screen actinomycetes for antimicrobial activity from mangroves and estuarine soil samples of Cochin. Methods: In the present study, sediment samples collected from mangroves and various stations of Cochin estuary were pretreated and actinomycetes were isolated on different selective media. The isolates were screened for antibiotic activity by following disc diffusion assay (Kirby-Bauer method) against human pathogens, fish pathogens and Gram-positive bacteria. The isolates were identified based on their morphology. Results:Only 2 actinomycete isolates (ER7and ER10) of the 50 isolates screened had antimicrobial activities against one or more pathogens tested. ER7 isolate showed higher antimicrobial activity as compared to that of ER10 isolate. The maximum inhibition zone of crude extract from ER7 was 16.7 mm. The methanol extract of ER7 showed antimicrobial activity against all the pathogens tested with a maximum zone of 21.0 mm. The isolates with antimicrobial activity were found to belong to the genusStreptomyces. Conclusions:There is no significant report on bioactive actinomycetes from the present study areas. Potent antibiotics from the selected isolates could contribute to fight against several human and fish diseases. Further purification, structural elucidation and characterization are recommended to know the quality, novelty and commercial value of these antibiotics. Hence, the mangroves and estuary of Kochi show great promise for the discovery of bioactive actinomycetes.

  12. Phosphate solubilizing activities of Actinomycetes isolated from Waigeo, Raja Ampat islands, West Papua

    SRI WIDAWATI

    2008-04-01

    Full Text Available Actinomycetes is a major microbial group observed in soil, and contributes to nutrient cycling. This study is intended to verify physiological characters and phosphate solubilizing ability of Actinomycetes isolated from soil of Raja Ampat, West Papua. Most of isolates (RCW16-9, RCW16-8, W5-6, RCW25-1, RCW26-5, W28-4, W3-1, W3-7, W17-7, and W10-1 belonged to Streptomyces genera. The isolates produce clear zone in Pivoskaya after 3 days incubation. The liquid growth of this isolate rapidly utilizes glucose, and after 24 days of incubation almost 95% glucose was consumed. Decrease of pH from 6.1 to 4.3 may stimulate dissolution of calcium phosphate, and about 21 mg/L-P was observed in bulk solution. An increase of phosphomonoesterase activity during incubation is concomitant with the release of orthophosphate into bulk solution. Acidity of cultures increased may stimulate solubilization of calcium phosphate. Most strains produce phosphomonoesterase enzyme, indicating that actinomycetes are important soil microbes responsible for mediation and stimulation of both inorganic and organic phosphate dissolution. Physiological and biomass growth character of phosphate solubilizing actinomycetes could be important taxonomic indicator for identifying and grouping soil actinomycetes.

  13. [Distribution and characteristics of soil antagonistic actinomycetes on northern slope of Taibai Mountain, Qinling].

    Zhu, Wen-Jie; Xue, Quan-Hong; Cao, Yan-Ru; Xue, Lei; Shen, Guang-Hui; Lai, Hang-Xian

    2011-11-01

    Twelve representative soil samples were collected from different altitudes on the northern slope of Taibai Mountain to study the distribution and characteristics of soil antagonistic actinomyces by using agar block method. There existed a great deal of soil antagonistic actinomyces in the study area. Among the 141 actinomycete strains isolated, 116 strains (82.3%) showed antagonism toward 12 target bacteria or fungi. The antagonistic strains at altitudes 800-1845, 3488, 3655, and 3670 m occupied 73.7% -86.8%, 81.3%, 78.9% and 82.3% of the total, respectively. 42.1% of the strains at altitudes 1200-2300 m and > 3400 m showed strong and broad spectrum antagonistic activity, suggesting that there was a great potential for the isolation of actinomycete strains with strong anti-biotic capability at these altitudes. 24.1% of the antagonistic actinomycetes showed antagonism against Staphyloccocus aureu, and 2.4%, 6.9% and 11.2% of them showed activity toward Verticillium dahliae in cotton, Phytophthora sp. in strawberry and Neonectria radiciccla in ginseng, respectively. This study showed that the soil actinomycete antagonistic potentiality (SAAP) could be used as a quantitative indicator to evaluate the potential of antagonistic actinomycete resources in soil. PMID:22303680

  14. SCREENING OF ANTIMICROBIAL ACTIVITY AND GENES CODING POLYKETIDE SYNTHETASE AND NONRIBOSOMAL PEPTIDE SYNTHETASE OF ACTINOMYCETE ISOLATES

    Silvia Kovácsová

    2013-12-01

    Full Text Available The aim of this study was to observe antimicrobial activity using agar plate diffusion method and screening genes coding polyketide synthetase (PKS-I and nonribosomal peptide synthetase (NRPS from actinomycetes. A total of 105 actinomycete strains were isolated from arable soil. Antimicrobial activity was demonstrated at 54 strains against at least 1 of total 12 indicator organisms. Antifungal properties were recorded more often than antibacterial properties. The presence of PKS-I and NRPS genes were founded at 61 of total 105 strains. The number of strains with mentioned biosynthetic enzyme gene fragments matching the anticipated length were 19 (18% and 50 (47% respectively. Overall, five actinomycete strains carried all the biosynthetical genes, yet no antimicrobial activity was found against any of tested pathogens. On the other hand, twenty-one strains showed antimicrobial activity even though we were not able to amplify any of the PKS or NRPS genes from them. Combination of the two methods showed broad-spectrum antimicrobial activity of actinomycetes isolated from arable soil, which indicate that actinomycetes are valuable reservoirs of novel bioactive compounds.

  15. Biosurfactant produced from Actinomycetes nocardiopsis A17: Characterization and its biological evaluation.

    Chakraborty, Samrat; Ghosh, Mandakini; Chakraborti, Srijita; Jana, Sougata; Sen, Kalyan Kumar; Kokare, Chandrakant; Zhang, Lixin

    2015-08-01

    This investigation aims to isolate an Actinomycetes strain producing a biosurfactant from the unexplored region of industrial and coal mine areas. Actinomycetes are selected for this study as their novel chemistry was not exhausted and they have tremendous potential to produce bioactive secondary metabolites. The biosurfactant was characterized and further needed to be utilized for pharmaceutical dosage form. Isolation, purification, screening, and characterization of the Actinomycetes A17 were done followed by its fermentation in optimized conditions. The cell-free supernatant was used for the extraction of the biosurfactant and precipitated by cold acetone. The dried precipitate was purified by TLC and the emulsification index, surface tension and CMC were determined. The isolated strain with preferred results was identified as Actinomycetes nocardiopsis A17 with high foam-forming properties. It gives lipase, amylase, gelatinase, and protease activity. The emulsification index was found to be 93±0.8 with surface tension 66.67 dyne/cm at the lowest concentration and cmc 0.6 μg/ml. These biosurfactants were characterized by Fourier transform infra red (FT-IR) spectroscopy and liquid chromatography-mass spectrometry (LC-MS). Therefore, it can be concluded that the biosurfactant produced by Actinomycetes nocardiopsis sp. strain A17 was found to have satisfactory results with high surface activity and emulsion-forming ability. PMID:25989147

  16. Screening of Actinomycetes From Lipar Area of Oman Sea to Investigate the Antibacterial Compounds

    Shams

    2015-02-01

    Full Text Available Background Actinomycetes are one of the most important sources for the production of antibacterial compounds. Marine environments, due to their unique characteristics, are considered a good option to search for bacteria with the capability of producing antimicrobial compounds. Objectives The purpose of this study was to isolate the actinomycetes producing antibacterial compounds. Materials and Methods A total of 35 actinomycetes were isolated from Oman Sea (Lipar Area. To investigate antibacterial activity, the isolated actinomycetes were assessed against reference and pathogenic bacteria, including Staphylococcus epidermidis, Staphylococcu intermedius, Staphylococcu chromogenes, Staphylococcu saprophyticus, Bacillus cereus and methicillin-resistance Staphylococcu aureus, Pseudomonas, Listeria, Klebsiella, Salmonella, Acinetobacter, and Escherichia coli O157:H7, using the cross streak method. Results Based on the morphological characterization, 35 isolated cases belonged to actinomycetes and %94 of them had the ability to produce antibacterial compounds. In the cross streak method, most of the isolated bacteria have antibacterial activity against reference S. aureus among Gram-positive bacteria and Acinetobacter among Gram-negative bacteria. Inhibition zone diameters were measured between 2-25 and 1-20 mm for Gram-positive and -negative bacteria, receptivity. Conclusions Preliminary results indicate that the native Iranian Actinobacteria could be considered a suitable option for screening of the new antibacterial compounds. Molecular research and antibacterial compound extraction against the aforementioned pathogenic strains are also being conducted.

  17. Amylase activity of aquatic actinomycetes isolated from the sediments of mangrove forests in south of Iran

    Farshid Kafilzadeh

    2015-01-01

    Full Text Available In this study amylase producing actinomycetes were isolated from the sediments of mangrove forests in the south of Iran and the rate of amylase activity was measured. The samples of sediments were collected from one hundred different places in mangrove forests of the south of Iran. Collected samples were diluted then they were purified on the starch (casein agar culture and Woodruff. After that they were examined in terms of amylase production on agar–starch culture. The activity of the produced amylase by the isolated aquatic actinomycetes was measured by dinitrosalicylic acid (DNS method. The results showed that aquatic actinomycetes were isolated from 86 per 100 places in spring (86% and from 61 per 100 places in summer (61%. The highest rates of producing enzyme were related to isolated samples in spring (62.97 U/ml. Biochemical and Bergey’s book tests showed that the most isolated aquatic actinomycetes belonged to Streptomyces genus. As regards this, it is economical and easy to isolate the aquatic actinomycetes which produce amylase that is used in different industries in Iran from the sediments of mangrove forests of the south of Iran. So the isolated strains in this study can be suitable candidates for amylase production after genetic manipulation.

  18. Phylogenetic characterization of culturable actinomycetes associated with the mucus of the coral Acropora digitifera from Gulf of Mannar.

    Nithyanand, Paramasivam; Manju, Sivalingam; Karutha Pandian, Shunmugiah

    2011-01-01

    The marine environment is a virtually untapped source of novel actinomycete diversity and its metabolites. Investigating the diversity of actinomycetes in other marine macroorganisms, like seaweeds and sponges, have resulted in isolation of novel bioactive metabolites. Actinomycetes diversity associated with corals and their produced metabolites have not yet been explored. Hence, in this study we attempted to characterize the culturable actinomycetes population associated with the coral Acropora digitifera. Actinomycetes were isolated from the mucus of the coral wherein the actinomycetes count was much higher when compared with the surrounding seawater and sediment. Actinobacteria-specific 16S rRNA gene primers were used for identifying the isolates at the molecular level in addition to biochemical tests. Amplified ribosomal DNA restriction analysis using three restriction enzymes revealed several polymorphic groups within the isolates. Sequencing and blast analysis of the isolates revealed that some isolates had only 96.7% similarity with its nearest match in GenBank indicating that they may be novel isolates at the species level. The isolated actinomycetes exhibited good antibacterial activity against various human pathogens. This study offers for the first time a prelude about the unexplored culturable actinomycetes diversity associated with a scleractinian coral and their bioactive capabilities. PMID:21105906

  19. Respuesta y conducta antidepredadora en lacértidos de ambientes aislados

    Mencía Rodríguez, Abraham

    2016-01-01

    [ES] El riesgo de depredación ha sido considerado desde hace tiempo como la mayor fuerza selectiva para la evolución de algunas características morfológicas y del comportamiento de los animales. Muchos organismos viven en un conflicto permanente entre diferentes demandas ecológicas y deben optimizar la búsqueda del alimento y la reproducción, al mismo tiempo que evitan ser atacados por un depredador. Por lo tanto, la depredación debe de ser una fuerza selectiva muy intensa para que evolucione...

  20. Ecological and Taxonomic Features of Actinomycetal Complexes in Soils of the Lake Elton Basin

    Zenova, G. M.; Dubrova, M. S.; Kuznetsova, A. I.; Gracheva, T. A.; Manucharova, N. A.; Zvyagintsev, D. G.

    2016-02-01

    In the sor (playa) solonchaks of chloride and sulfate-chloride salinity (the content of readily soluble salts is 0.9-1.0%) in the delta of the Khara River discharging into Lake Elton, the number of mycelial actinobacteria (actinomycetes) is low ((2-3) × 103 CFU/g of soil). At a distance from the water's edge, these soils are substituted for the light chestnut ones, for which an elevated number of actinomycetes (an order of magnitude higher than in the sor solonchaks) and a wider generic spectrum are characteristic. The actinomycetal complex is included the Streptomyces and Micromonospora genera, whereas in the sor solonchaks around the lake, representatives of Micromonospora were not found.

  1. Cellulolytic Actinomycetes isolated from soil in Bukit Duabelas National Park, Jambi

    ATIT KANTI

    2005-04-01

    Full Text Available The objective of study was to investigate the enzymatic activities of cellulolytic Actinomycetes. The soil sample was collected from Bukit Duabelas National Park, Jambi. Actinomycetes was isolated by Casein Agar Medium, and its cellulolytic capacity was determined by measuring the activity of CMC-ase. Two isolates of cellulolytic Actinomycetes belonged to genus Streptomyces were isolated. The CMC-ase activity was 7.7 unit and 13.4 unit for isolate I and isolate II respectively. The Km of isolate I and isolate II were 1.356x 10-3 and 1.595x 10-3 (% b/v respectively. Vmaks of isolate I and II was 1.658 x10-4 and 6.166x 10-4 µg glukosa/mL enzyme /minute, respectively.

  2. Actinomycetal complexes in drained peat soils of the taiga zone upon pyrogenic succession

    Zenova, G. M.; Glushkova, N. A.; Bannikov, M. V.; Shvarov, A. P.; Pozdnyakov, A. I.; Zvyagintsev, D. G.

    2008-04-01

    The number and diversity of actinomycetes in peat soils vary in dependence on the stage of pyrogenic succession. In the cultivated peat soil, the number of actinomycetes after fires decreases by three-four times, mainly at the expense of acidophilic and neutrophilic groups. An increase in the number of mycelial prokaryotes (at the expense of alkaliphilic forms) is seen on the fifth year of functioning of the pyrogenic peat soil. The species diversity of streptomycetes in peat soils also decreases after fires. An increase in the range of streptomycetal species at the expense of neutrophilic and alkaliphilic forms takes place on the fifth year of the pyrogenic succession. Parameters of the actinomycetal complex—the population density, species composition, and ecological features—are the criteria whose changes allow us to judge the state of peat soils in the course of their pyrogenic succession.

  3. A Search and Improvement of Actinomycetes Strains by Gamma Radiation for Biological Control of Plant Pathogen

    Two hundred isolates of actinomycetes isolate from soil sample in Sakaerat Bioshere Reserve and Suwanvajokkasikit Field Corps Research Station were tested the ability of actinomycetes on chitinase production and inhibition on the growth of 3 phyto pathogenic fung: Fusarium sporotrichiodes, Rhizoctonia solani and Sclerotium rolfsii, It was found that 7 isolates showed good tendency to control the growth of phyto pathogenic fungi in the with and with chitin. To increase the ability on antifungal activity the select show were mutant using gamma irradiation of radiance 173 isolates of mutant actinomycetes we found that only 35 isolates showed higher in inhibitory effect on three phyto pathogenic fungi tested. three isolate. Three isolates of mutant strains, SJ9I-15, SG4I-17 and SG4I-38 and two isolates of wild type strain which are SJ9 and SG4 were selected for controlling phyto pathogenic fungi in the green house.

  4. Efecto de aislados bacterianos de la familia de las rhizobacterias provenientes de alfalfa (Medicago sativa) en la mejora del crecimiento de plantas de frijol (Phaseolus vulgaris) y nodulación en jitomate (Solanum lycopersicon)

    Alma Lilián Guerrero Barrera; Cristina Garcidueñas Piña; Ilse Patricia Rivas Valdéz; Ezequiel Alejandro Madrigal Carrillo; José Francisco Morales Domínguez

    2009-01-01

    Se evaluó el efecto de aislados de rhizobacterias provenientes de alfalfa (Medicago sativa L.), en el crecimiento y expresión de proteínas en plantas de frijol (Phaseolus vulgaris) y jitomate (Solanum lycopersicon). Se obtuvieron dos diferentes aislados; A y Ab, los cuales se inocularon en semillas de frijol Bayo, Pinto y Negro y de jitomate Bola y Río Grande. Los mejores resultados en crecimiento se observaron en el frijol Pinto y el Bayo con los aislados A y Ab, respectivamente. En el jito...

  5. Resistencia antibiótica asociada a integrones de clase 1 en aislados humanos de enterobacterias de dos contextos epidemiológicos: zoonosis por "Salmonella enterica" e infección por "Klebsiella pneumoniae" adquirida en un centro sociosanitario

    Pérez Moreno, Mª del Mar

    2011-01-01

    [spa] OBJETIVO: Establecer la contribución de los integrones de clase 1 a la resistencia antibiótica en aislados clínicos de enterobacterias de dos contextos epidemiológicos: zoonosis por Salmonella enterica e infección por Klebsiella pneumoniae resistente a amoxicilina/clavulánico (ACL) adquirida en un centro sociosanitario. AISLADOS: Se estudiaron (a) 92 aislados humanos de Salmonella enterica serotipo Typhimurium (ST) recuperados entre 2004 y 2006 en el laboratorio de microbiología del ...

  6. Exploration of Potential Actinomycetes from CIFOR Forest Origin as Antimicrobial, Antifungus, and Producing Extracellular Xylanase

    Sipriyadi Sipriyadi

    2016-03-01

    Full Text Available This study aimed to isolate and explore the actinomycetes of CIFOR forest origin as an antimicrobial and antifungal agent, to produce an extracellular xylanase, and to identify isolates based on 16S rRNA gene sequences. Actinomycetes were isolated using Humic-acid Vitamin-B agar (HV media. Actinomycetes colonies that grow on the medium HV was subsequently purified by growing them on yeast malt agar (YMA media, then an antagonistic test of selected bacteria against Bacillus sp., Escherichia coli, Fusarium oxysporum, and Sclerotium sp was performed. Xylanase activity test was detected by observing a clear zone, followed by identification. Total of 35 isolates of actinomycetes isolated based on their colony morphology characteristics and diverse types of spore chains showed Streptomyces spp. of isolates CFR-06, CFR-15, CFR-17, CFR-18, and CFR-19 were able to inhibit the growth of Bacillus sp.. The highest inhibition zone has a diameter of 10.1 mm (isolate CFR-17. Isolates CFR-01 and CFR-15 were able to inhibit the growth of E. coli with the highest inhibition zone diameter of 5.1 mm (isolate CFR-15. Isolates CFR-29 and CFR-12 were able to inhibit the growth of F. oxysporum while isolate CFR-35 were able to inhibit the growth of Sclerotium sp.. Xylanase activity test showed that isolates CFR-12, CFR-20, CFR-22, CFR-24, CFR-25, CFR-30, CFR-33, CFR-34 have an ability to produce extracellular xylanase enzyme. Actinomycetes isolate (Xyl_22 as a potential xylanase enzyme producer was closely related with Streptomyces drozdowicii by the maximum similarity of 99%.How to CiteSipriyadi, S., Lestari, Y., Wahyudi, A., & Meryandini, A. (2016. Exploration Potential CIFOR Forest actinomycetes origin as Antimicrobial, Anti Fungus and Producing Enzymes Extracellular Xylanase. Biosaintifika: Journal of Biology & Biology Education, 8(1, 94-102.

  7. Antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India

    Hotam S Chaudhary

    2013-01-01

    Full Text Available The main objective of the present study was isolation, purification, and characterization of actinomycetes from soil samples, having antimicrobial activity against 12 selected pathogenic strains. Soils samples were taken from different niche habitats of Sheopur district, Madhya Pradesh, India. These samples were serially diluted and plated on actinomycete isolation agar media. Potential colonies were screened, purified, and stored in glycerol stock. Isolates were morphologically and biochemically characterized. These isolates were subjected to extraction for production of the antibacterial compound. Antibacterial activity and Minimum Inhibitory Concentration (MIC of the purified extract of isolates were evaluated. Totally 31 actinomycete isolates were tested for antagonistic activity against 12 pathogenic microorganisms. Isolates AS14, AS27, and AS28 were highly active, while AS1 showed less activity against the pathogenic microorganisms. Isolate AS7 exhibited the highest antagonistic activity against Bacillus cereus (24 mm and AS16 showed the highest activity against Enterococcus faecalis (21 mm. MIC was also determined for actinomycete isolates against all the tested microorganisms. MIC of actinomycete isolates was found to be 2.5 mg/ml against Shigella dysenteriae, Vancomycin-resistant enterococci, and Klebsiella pneumoniae, and was 1.25 mg/ml for Staphylococcus saprophyticus, Streptococcus pyogenes, Staphylococcus epidermidis, Methicillin-resistant Staphylococcus, Bacillus cereus, Staphylococcus xylosus, Methicillin-resistant Staphylococcus aureus, Enterococcus faecalis, and Staphylococcus aureus. All actinomycetes isolates showed antibacterial activity against S. aureus, while they showed less activity against S. dysenteriae. These isolates had antibacterial activity and could be used in the development of new antibiotics for pharmaceutical or agricultural purposes.

  8. Crystallochemical transformation of phyllosilicates under the impact of cyanobacteria and actinomycetes

    Chizhikova, N. P.; Omarova, E. O.; Lobakova, E. S.; Zenova, G. M.; Manucharov, A. S.

    2009-01-01

    The transformation of the crystallochemical structure of phyllosilicates (vermiculite and biotite) under the impact of growing cyanobacterial and actinomycetal associations, as well as monocultures of cyanobacteria and actinomycetes, has been studied. The character of the mineral transformation depends on their crystallochemical structure and the type of biota. The most significant changes take place in the contact zone between minerals and microbial associations; the effect of microbial monocultures is smaller. The transformation of biotite proceeds via the stage of ordered mica-vermiculite (smectite) neoformation (rectorite); the destruction of vermiculite has been identified.

  9. Draft genome sequences of three chemically rich actinomycetes isolated from Mediterranean sponges

    Horn, Hannes; Cheng, Cheng; Edrada-Ebel, RuAngelie; Hentschel, Ute; Abdelmohsen, Usama Ramadan

    2015-01-01

    Metabolomic analysis has shown the chemical richness of the sponge-associated actinomycetes Streptomyces sp. SBT349, Nonomureae sp. SBT364, and Nocardiopsis sp. SBT366. The genomes of these actinomycetes were sequenced and the genomic potential for secondary metabolism was evaluated. Their draft genomes have sizes of 8.0, 10, and 5.8Mb having 687, 367, and 179 contigs with a GC content of 71.6, 70.7, and 72.7%, respectively. Moreover, antiSMASH 3.0 predicted 108, 149, and 75 secondary metabol...

  10. Phosphate solubilizing activities of Actinomycetes isolated from Waigeo, Raja Ampat islands, West Papua

    SRI WIDAWATI; ARIF NURKANTO; I Made Sudiana

    2008-01-01

    Actinomycetes is a major microbial group observed in soil, and contributes to nutrient cycling. This study is intended to verify physiological characters and phosphate solubilizing ability of Actinomycetes isolated from soil of Raja Ampat, West Papua. Most of isolates (RCW16-9, RCW16-8, W5-6, RCW25-1, RCW26-5, W28-4, W3-1, W3-7, W17-7, and W10-1) belonged to Streptomyces genera. The isolates produce clear zone in Pivoskaya after 3 days incubation. The liquid growth of this isolate rapidly uti...

  11. Screening of Antibacterial Producing Actinomycetes from Sediments of the Caspian Sea

    Mohseni, Mojtaba; Norouzi, Hamed; Hamedi, Javad; Roohi, Aboulghasem

    2013-01-01

    Actinomycetes are interesting as a main producer of secondary metabolites and industrial antibiotics from marine environments. A total of 44 strains of actinomycetes were isolated from Caspian Sea sediments at a depth of 5-10 m. Preliminary screening was done using cross-streak method against 2 gram-positive and 4 gram-negative pathogen bacteria. The most potent strains MN2, MN3, MN38, MN39, MN40, MN41, and MN44 were used to extract the antibacterial substances. The antibacterial activities w...

  12. ISOLATION AND CHARACTERIZATION OF BIOACTIVE ACTINOMYCETES FROM SOIL IN AND AROUND NAGPUR

    Rita N. Wadetwar* and Arun T. Patil

    2013-01-01

    ABSTRACT: Fifteen soil samples were collected from different locations near Nagpur i.e. from Nagpur, Bhandara and Chandrapur. Air drying and pre-treatment of soil samples with CaCo3 (1%) were found to be most effective for isolation of actinomycetes. Five different selective media were used for the isolation of actinomycetes and the best growth was observed in yeast malt extract agar medium with 1 mg/ml cycloheximide as antifungal agent and by using double layer agar technique for isolation. ...

  13. Isolation and Characterization of Actinomycete Antagonists of a Fungal Root Pathogen †

    Crawford, Don L.; Lynch, James M.; Whipps, John M.; Ousley, Margaret A.

    1993-01-01

    By use of selective media, 267 actinomycete strains were isolated from four rhizosphere-associated and four non-rhizosphere-associated British soils. Organic media with low nutrient concentrations were found to be best for isolating diverse actinomycetes while avoiding contamination and overgrowth of isolation media by eubacteria and fungi. While all isolates grew well at pHs 6.5 to 8.0, a few were unable to grow at pH 6.0 and a significant number failed to grow at pH 5.5. Eighty-two selected...

  14. Digestibilidad de nutrimentos en lechones alimentados con dietas con aislado o concentrado de proteína de soya

    Maria de los Angeles Aguilera Barreiro; Tércia Cesária Reis de Souza; Gerardo Mariscal Landín; Arturo Germán Borbolla Sosa; Araceli Aguilera Barreyro

    2006-01-01

    Se utilizaron 15 lechones destetados a los 17 días con 6.1 ± 1.8 kg para medir el efecto de la fuente de proteína sobre la digestibilidad de tres dietas, una con concentrado de proteína de soya (CPS) y las otras dos con aislado de proteína de soya solo (APS) o con suero de leche (APSSL). Se determinó la digestibilidad ileal y total aparente (DIA y DTA) de materia seca (MS), proteína cruda (PC) y energía (En) en tres periodos (P): P1= 26-29; P2= 33-36 y P3= 40-43 días. La DIA-MS fue mayor (P0....

  15. Glucose metabolism in the antibiotic producing actinomycete Nonomuraea sp ATCC 39727

    Gunnarsson, Nina; Bruheim, Per; Nielsen, Jens

    2004-01-01

    The actinomycete Nonomuraea sp. ATCC 39727, producer of the glycopeptide A40926 that is used as precursor for the novel antibiotic dalbavancin, has an unusual carbon metabolism. Glucose is primarily metabolized via the Entner-Doudoroff (ED) pathway, although the energetically more favorable Embde...

  16. Isolation and identification of actinomycetes from a compost-amended soils biocontrol agents

    Compost capability to suppress soil-borne plant pathogens has become an interesting subject as a strategy for reducing the adverse effects of massive fungicides application in the environmental. In this context, actinomycetes have received considerable attention as biocontrol agents, particularly Streptomyces species. (Author)

  17. Isolation and identification of actinomycetes from a compost-amended soils biocontrol agents

    Garcia de la Fuente, R.; Cuesta, G.; Fornes, F.; Abad, M.

    2009-07-01

    Compost capability to suppress soil-borne plant pathogens has become an interesting subject as a strategy for reducing the adverse effects of massive fungicides application in the environmental. In this context, actinomycetes have received considerable attention as biocontrol agents, particularly Streptomyces species. (Author)

  18. Screening of chitinolytic actinomycetes for biological control of Sclerotium rolfsii stem rot disease of chilli

    Pranee Pattanapipitpaisal

    2012-09-01

    Full Text Available Two hundred and eighty three strains were isolated from rhizoshere-associated soils, from Ubon Ratchathani andSrisaket province, using Enrichment Media for isolation of Chitinase-producing Actinomycetes agar (EMCA agar. All strainswere screened for chitinolytic activity and sixty eight strains gave significant clear zone on EMCA agar plates. The selectedchitinolytic strains were assayed for in vitro antagonism against Sclerotium rolfsii using cornmeal agar (CMA agar assayprocedure and the result showed that thirteen isolates have remarkable inhibiting the growth of the fungus and the top fiveantagonistic actinomycetes were PACCH 277, PACCH129, PACCH225, PACCH24 and PACCH246, respectively. The resultindicated that these actinomycetes produce chitinase which catalyze the degradation of chitin, resulting in inhibition of S.rolfsii growth. Their abilities to control the disease development were tested for in vivo biocontrol assay on chilli seedlings.Two out of thirteen candidate, PACCH24 and PACCH225, antagonists reduced the disease development at 90%. It wassuggested that the ability to inhibit the growth of pathogen in vitro was not related to the disease reduction in vivo. Thestrain PACCH24 was further identified as Streptomyces hygroscopicus according to morphological characteristic, cell walland cellular sugar analysis and 16S rDNA sequencing. The study implies a novel chitinolytic actinomycete which could bedeveloped to be a biological agent which would be included as a complement with organic fertilizers in order to control stemrot disease and promote growth of chilli.

  19. Effect of gamma radiation on the survival of fungal and actinomycetal florae contaminating medicinal plants

    This study evaluates the effect of gamma radiation on the viability of fungi and actinomycetes that contaminate medicinal plants. The relationship between the total lipids of some fungi and actinomycetes and their sensitivity to gamma radiation is also investigated. The data reveal that the viable counts of these florae decrease approximately exponentially with the radiation dose, the effective dose for the elimination of these microorganisms being about 5 kGy for all the medicinal plants under study. Response of pure cultures of fungi and actinomycetes isolated from medicinal plants to increasing absorbed doses of gamma radiation indicate that an increase in radioresistance is in the following order: Streptomyces rimosus, Fusarium solani, Nocardia kuroishii. F. oxysporum, A. fumigatus, A. flavus, A. parasiticus and A. ochraceus. The total lipid contents of molds and actinomycetes have been reported to be increased by increasing the radio-resistance of microorganisms, and hence there is a relationship between the radio-sensitivity of microorganisms and the total lipid mass of flora mycelia. (Author)

  20. Anthracimycin, a potent anthrax antibiotic from a marine-derived actinomycete.

    Jang, Kyoung Hwa; Nam, Sang-Jip; Locke, Jeffrey B; Kauffman, Christopher A; Beatty, Deanna S; Paul, Lauren A; Fenical, William

    2013-07-22

    Licensed to kill: A new antibiotic, anthracimycin (see scheme), produced by a marine-derived actinomycete in saline culture, shows significant activity toward Bacillus anthracis, the bacterial pathogen responsible for anthrax infections. Chlorination of anthracimycin gives a dichloro derivative that retains activity against Gram-positive bacteria, such as anthrax, but also shows activity against selected Gram-negative bacteria. PMID:23776159

  1. Enumerating actinomycetes in compost bioaerosols at source—Use of soil compost agar to address plate 'masking'

    Taha, M. P. M.; Drew, G. H.; Tamer Vestlund, A.; Aldred, D.; Longhurst, P. J.; Pollard, S. J. T.

    Actinomycetes are the dominant bacteria isolated from bioaerosols sampled at composting facilities. Here, a novel method for the isolation of actinomycetes is reported, overcoming masking of conventional agar plates, as well as reducing analysis time and costs. Repeatable and reliable actinomycetes growth was best achieved using a soil compost media at an incubation temperature of 44 °C and 7 days' incubation. The results are of particular value to waste management operators and their advisors undertaking regulatory risk assessments that support environmental approvals for compost facilities.

  2. A mixed community of actinomycetes produce multiple antibiotics for the fungus farming ant Acromyrmex octospinosus

    Barke Jörg

    2010-08-01

    Full Text Available Abstract Background Attine ants live in an intensely studied tripartite mutualism with the fungus Leucoagaricus gongylophorus, which provides food to the ants, and with antibiotic-producing actinomycete bacteria. One hypothesis suggests that bacteria from the genus Pseudonocardia are the sole, co-evolved mutualists of attine ants and are transmitted vertically by the queens. A recent study identified a Pseudonocardia-produced antifungal, named dentigerumycin, associated with the lower attine Apterostigma dentigerum consistent with the idea that co-evolved Pseudonocardia make novel antibiotics. An alternative possibility is that attine ants sample actinomycete bacteria from the soil, selecting and maintaining those species that make useful antibiotics. Consistent with this idea, a Streptomyces species associated with the higher attine Acromyrmex octospinosus was recently shown to produce the well-known antifungal candicidin. Candicidin production is widespread in environmental isolates of Streptomyces, so this could either be an environmental contaminant or evidence of recruitment of useful actinomycetes from the environment. It should be noted that the two possibilities for actinomycete acquisition are not necessarily mutually exclusive. Results In order to test these possibilities we isolated bacteria from a geographically distinct population of A. octospinosus and identified a candicidin-producing Streptomyces species, which suggests that they are common mutualists of attine ants, most probably recruited from the environment. We also identified a Pseudonocardia species in the same ant colony that produces an unusual polyene antifungal, providing evidence for co-evolution of Pseudonocardia with A. octospinosus. Conclusions Our results show that a combination of co-evolution and environmental sampling results in the diversity of actinomycete symbionts and antibiotics associated with attine ants.

  3. Estudio de aislados no patogénicos de Fusarium oxysporum para el control biológico de patógenos fúngicos oportunistas

    Arroyo Gallardo, Víctor

    2015-01-01

    Los hongos oportunistas afectan numerosas especies vegetales tanto en los cultivos como en productos almacenados. Este tipo de enfermedades son muy difíciles de combatir mediante el empleo de fitosanitarios. La aplicación de los mismos se encuentra muy limitada y es especialmente importante en productos destinados a consumo. Por ello, en el presente estudio planteamos el control biológico como alternativa. Para conseguir este objetivo, trabajamos con siete aislados no patogénicos de Fus...

  4. Patrones de susceptibilidad antimicrobiana de aislados clínicos de Staphylococcus aureus procedentes de la comunidad

    Estrella Alvarez Varela

    2008-01-01

    Full Text Available Se estudió la resistencia antimicrobiana de 695 aislamientos clínicos de Staphylococcus aureus procedentes de infecciones de las vías respiratorias superiores, lesiones en piel, secreciones de heridas, exudados conjuntivales, óticos y sistema genitourinario de pacientes atendidos en consulta externa de cinco hospitales pediátricos, ubicados en diferentes ciudades y regiones de Cuba durante el período enero de 2002 a diciembre de 2004. Los datos fueron obtenidos por el Sistema DIRAMIC y procesados por el sistema de programas para la confección de Mapas Microbianos, versión 6.0. Los antibióticos probados fueron: penicilina G, oxacilina, cefazolina, gentamicina, amicacina, tetraciclina, cloranfenicol, eritromicina, ciprofloxacina y sulfametoxazol + trimetoprim. Los aislados fueron clasificados sobre la base de su resistencia a la oxacilina. De ellos, 118 (17,0 % fueron resistentes al fármaco (Staphylococcus aureus meticilina resistente, SAMR y 577 (83,0 % sensibles (Staphylococcus aureus meticilina sensible, SAMS. Se observó un incremento significativo de las cepas SAMR en el período y de la resistencia dentro de este grupo al resto de los antibióticos probados, aunque más del 50 % de los aislados conservan la susceptibilidad a macrólidos, aminoglucósidos y quinolonas. Por su parte, los porcentajes de resistencia de las cepas sensibles fueron bajos y muy similares en los tres años para la mayoría de los antibióticos ensayados, con excepción de eritromicina y ciprofloxacina, para los que se observaron incrementos en el año 2004. Los resultados revelaron que en Cuba los Staphylococcus aureus resistentes a oxacilina aún no son un problema. Pero la aparición de este germen en la comunidad en una frecuencia cercana al 20 %, hace necesario mantener la vigilancia de este fenómeno, lo cual permitirá tomar a tiempo las medidas pertinentes para evitar sus consecuencias negativas.

  5. Specificity of the mutualistic association between actinomycete bacteria and two sympatric species of Acromyrmex leaf-cutting ants

    Poulsen, M; Cafaro, M; Boomsma, J J;

    2005-01-01

    Acromyrmex leaf-cutting ants maintain two highly specialized, vertically transmitted mutualistic ectosymbionts: basidiomycete fungi that are cultivated for food in underground gardens and actinomycete Pseudonocardia bacteria that are reared on the cuticle to produce antibiotics that suppress...

  6. Diversity of Actinomycetes at Several Forest Types in Wanagama I Yogyakarta and Their Potency as a Producer of Antifungal Compound

    Reni Nurjasmi

    2015-11-01

    Full Text Available Actinomycetes are bacterial groups that produce many secondary metabolites, which different biological activities, such as antifungi, antibacteria, antivirus, antitumor, etc. Actinomycetes are widely distributed in soil and their diversity is influenced by type of forest. The aim of this study is to investigate diversity of actinomycetes in several forest types of Wanagama I forest in Yogyakarta and their potency as a producer of antifungal compound. Soil samples under the forest of Tectona grandis, Swietenia macrophylla King, Bamboosa vulgaris, Melaleuca leucadendron, and Gliricidia maculata were used as sources of soil bacteria. Bacteria and actinomycetes communities were analyzed through culture-independent approach by RISA and nested-PCR RISA using actinomycetes spesific primer (F243, respectively. Through culture-dependent approach, isolated actinomycetes diversity were analyzed by identification of morphology (colony and cell, genetic (BOX element by rep-PCR, and secondary metabolites (thin layer chromatography. In addition, isolates were assayed for their antifungal activity against Saccharomyces cerevisae, Candida albicans, Fusarium oxysporum and Aspergillus flavus. The presence of Polyketide Synthase-I (PKS-I and NonRibosomal Peptide Synthetase (NRPS genes were amplified by PCR to study their correlation with antifungal activity of the actinomycete isolates. The results showed that types of forest influence diversity of rhizobacteria especially actinomycetes. According to culture-independent approach, relatively, com-

munity of rhizobacteria from the highest were soil under the forest of B. vulgaris, G. maculata, T. grandis, S.macrophylla King, and M. leucadendron, respectively. Meanwhile, community of actinomycetes from the highest were soil under the forest of G. maculata, B. vulgaris, M. leucadendron, S. macrophylla King, and T. grandis, respec- tively. Fourty-three morphologically different isolates were found by using

  • Identification of soil actinomycetes in bukit bangkirai fire forest east kalimantan and its potention as cellulolitic and phosphate solubilizing

    ARIF NURKANTO

    2007-01-01

    Some actinomycetes which were isolated from Bukit Bangkirai fire forest in East Kalimantan have been identified. Those isoates were also characterized for its cellulolitic and phosphate solubilizing ability. Identification was based on Miyadoh (1997) and Holt (1994) methods. Actinomycetes could be identified by microscopic observation on spores, chain spore, hypha, aerial hypha and its pigmentation. The cellulolitic ability was observed by clear zone ratio in CMC medium and phosphate solubil...

  • Succession of Actinomycetes During Composting Proccess of Dairy-Farm Waste Investigated by Culture-Dependent and Independent Approaches

    Mukhlissul Faatih; Jaka Widada; Ngadiman N

    2015-01-01

    Mesophilic, thermophilic, and maturation phases were recognized in composting proccess. Temperaturechanges influence the microbial communities in compost within composting proccess. Actinomycetes account for alarger part of compost microbial population. The aim of this research was to study succession of actinomycetescommunity during composting of dairy-farm waste investigated by culture-dependent and independentapproaches.In culture-independent method, the succession of actinomycetes communi...

  • Phylogenetic appraisal of antagonistic, slow growing actinomycetes isolated from hypersaline inland solar salterns at Sambhar salt Lake, India

    SolomonRobinsonDavidJebakumar

    2013-01-01

    Inland solar salterns established in the vicinity of Sambhar Lake are extreme saline environments with high salinity and alkalinity. In view of the fact that microbes inhabiting such extreme saline environments flourish the contemporary bioprospecting, it was aimed to selectively isolate slow growing and rare actinomycetes from the unexplored solar salterns. A total of 14 slow growing actinomycetes were selectively isolated from three composite soil samples of inland solar salterns. Among the...

  • Use of the BioMerieux ID 32C yeast identification system for identification of aerobic actinomycetes of medical importance.

    Muir, D B; Pritchard, R C

    1997-01-01

    The BioMerieux ID 32C Yeast Identification System was examined to determine its usefulness as a rapid method for the identification of medically important aerobic actinomycetes. More than 290 strains were tested by this method and the results were compared to those obtained by conventional methods. It was found that aerobic actinomycetes could be differentiated to species level in 7 days by the ID 32C system.

  • In Vitro and In Vivo Plant Growth Promoting Activities and DNA Fingerprinting of Antagonistic Endophytic Actinomycetes Associates with Medicinal Plants

    Ajit Kumar Passari; Vineet Kumar Mishra; Vijai Kumar Gupta; Mukesh Kumar Yadav; Ratul Saikia; Bhim Pratap Singh

    2015-01-01

    Endophytic actinomycetes have shown unique plant growth promoting as well as antagonistic activity against fungal phytopathogens. In the present study forty-two endophytic actinomycetes recovered from medicinal plants were evaluated for their antagonistic potential and plant growth-promoting abilities. Twenty-two isolates which showed the inhibitory activity against at least one pathogen were subsequently tested for their plant-growth promoting activities and were compared genotypically using...

  • Analysis of similarity of the SI gene in infectious bronchitis virus (IBV isolates in Shanghai, China Análisis de similaridad del gen S1 de aislados del virus de la bronquitis infecciosa (IBV en Shanghai, China

    J G Zhu

    2007-01-01

    una longitud de 1626 bp y codifican 541 aminoácidos residuales. Al comparar con la secuencia de las cepas de referencia de IBV seleccionadas publicadas en GenBank, las 4 cepas de gallinas exhibieron 77,4%-82,9% de identidad en la secuencia del gen SI, con una similaridad deducida de la secuencia proteica de 74,7%-82,6%, mientras que la cepa de paloma exhibió un 79,3%-99,6% de similaridad en la secuencia del gen SI con una identidad deducida de la secuencia proteica de 81,6%-99,6%. La secuencia del sitio de división de la proteína SI de estos aislados contiene 5 aminoácidos básicos consecutivos, llamados Arg-Arg-Phe-Arg-Arg (RRFRR, similares a la mayoría de las cepas IBV. El análisis de los resultados indicó que el rango de variabilidad de la estructura del gen S1 es alto y corresponde al carácter biológico de IBV y, molecularmente, las cinco cepas aisladas en Shangai se relacionan cercanamente con las cepas IBV de referencia. Se concluyó que las cinco cepas aisladas pertenecen al IBV, aun cuando una fue aislada desde palomas

  • Inhibition of norsolorinic acid accumulation to Aspergillus parasiticus by marine actinomycetes

    Yan, Peisheng; Shi, Cuijuan; Shen, Jihong; Wang, Kai; Gao, Xiujun; Li, Ping

    2014-11-01

    Thirty-six strains of marine actinomycetes were isolated from a sample of marine sediment collected from the Yellow Sea and evaluated in terms of their inhibitory activity on the growth of Aspergillus parasiticus and the production of norsolorinic acid using dual culture plate assay and agar diffusion methods. Among them, three strains showed strong antifungal activity and were subsequently identified as Streptomyces sp. by 16S rRNA gene sequencing analysis. The supernatant from the fermentation of the MA01 strain was extracted sequentially with chloroform and ethyl acetate, and the activities of the extracts were determined by tip culture assay. The assay results show that both extracts inhibited mycelium growth and toxin production, and the inhibitory activities of the extracts increased as their concentrations increased. The results of this study suggest that marine actinomycetes are biologically important for the control of mycotoxins, and that these bacteria could be used as novel biopesticides against mycotoxins.

  • Isolation and partial characterization of actinomycetes with antimicrobial activity against multidrug resistant bacteria

    Smriti Singh; Pramod Kumar; N Gopalan; Bhuvnesh Shrivastava; RC Kuhad; Hotam Singh Chaudhary

    2012-01-01

    Objective: To isolate strains of Actinomycetes from different locations of Gwalior to evaluate its antimicrobial activity against multidrug resistant pathogenic strains. Method: Soil samples collected from different niche habitats of Gwalior were serially diluted and plated on selective media. Potential colonies were further purified and stored in agar slants and glycerol stocks. Isolates were biochemically characterized and purified isolates were test against pathogenic microorganisms for screening. Isolates with antagonistic properties were inoculated in production media and secondary metabolites or antimicrobial products were extracted. Result: The seven actinomycetes strains showing maximum antibacterial activity were isolated further characterized based on their colony characteristics and biochemical analyses. The isolates were screened for their secondary metabolites activity on three human pathogenic bacteria are Escherichia coli (E. coli), Methicillin-Resistant Staphylococcus aureus (S. aureus) and Vancomycin-Resistant Enterococci (VRE). Discussion: The strain MITS 1005 was found to be more active against the test bacteria.

  • Mutational analysis of primary alcohol metabolism in the methylotrophic actinomycete Amycolatopsis methanolica

    Hektor, Harm J.; Dijkhuizen, Lubbert

    1996-01-01

    Mutants of the methylotrophic actinomycete Amycolatopsis methanolica unable to grow on methanol as carbon source were isolated and characterized. Mutants specifically affected in methanol utilization were deficient in formaldehyde assimilation. Mutants blocked in the first step of primary alcohol oxidation (C1-C4) had lost activity of the tetrazolium-dependent alcohol dehydrogenase, a three-component enzyme complex. This complex, or individual components, thus play a crucial role in utilizati...

  • Isolation and screening of phytotoxin producing actinomycetes and determination of phytotoxin effect spectrum of selected strains

    Reyhan khayatmaher; Mohammad Ali Amoozegar; Shimasadat Seyedmahdi; Javad Hamedi; Mohammad Reza Naghavi; Faranak Foroozanfar; Ali Mohammad Latifi

    2012-01-01

    Introduction: Actinomycetes are well-known phytotoxin producing microorganisms. Nowadays the use of microbial herbicides versus traditional chemicals draws a great deal of attention as they do not cause any environmental problems.Materials and Methods: Actbacterial strains of 40 rhizospheric and phyllospheric samples were isolated. Primary screening was implemented on radish and cress seeds on GAP¬Agar. Then, bioassay of cell¬free broth of strains with more than 70% inhibitory effect on the s...

  • Bioleaching of Rare Earth Elements and Uranium From Sinai Soil, Egypt Using Actinomycetes

    Maha Amin Hewedy, Abeer Ahmed Rushdy, and Noha Mohammed Kamal

    2013-01-01

    The use of microorganisms in the recovery of Rare Earth Elements (REEs) and Uranium (U) from low grade ores provides an appropriate eco-friendly alternative to chemical methods used in extraction and/or removal of metals from soils, with a higher efficiency, less destruction to the environment, and lower cost from a commercial view especially given that high-grade mineral resources that can be used in the chemical extraction of metals are being depleted. The feasibility of using actinomycetes...

  • Actinobase: Database on molecular diversity, phylogeny and biocatalytic potential of salt tolerant alkaliphilic actinomycetes

    Sharma, Amit K; Gohel, Sangeeta; Singh, Satya P.

    2012-01-01

    Actinobase is a relational database of molecular diversity, phylogeny and biocatalytic potential of haloalkaliphilic actinomycetes. The main objective of this data base is to provide easy access to range of information, data storage, comparison and analysis apart from reduced data redundancy, data entry, storage, retrieval costs and improve data security. Information related to habitat, cell morphology, Gram reaction, biochemical characterization and molecular features would allow researchers...

  • Characterizing contrasting soils and sediments with semispecific actinomycete r-DNA PCR primers

    Hill, P.; Krištůfek, Václav; Caballero, S.; Kroetsch, D. J.; Rauch, Ota

    České Budějovice: Institute of Soil Biology AS CR, 2004, s. 88-92. ISBN 80-86525-03-1. [Present methods for investigation of microbial community biodiversity in soils and substrates. Methodological workshop /9./. České Budějovice (CZ), 02.03.2004-03.03.2004] Institutional research plan: CEZ:AV0Z6066911 Keywords : contrasting soils * sediments * semispecific actinomycete r-DNA PCR primers Subject RIV: EH - Ecology, Behaviour

  • Using actinomycete-specific PCR primers to characterize the bacterial communities of wormcasts

    Hill, P.; Krištůfek, Václav; Feijoo, A. M.; Gallego, G.

    České Budějovice: Institute of Soil Biology ASCR, 2005, s. 25-29. ISBN 80-86525-04-X. [Contributions to soil Zoology in Central Europe I. Central European Workshop on Soil Zoology /7./. České Budějovice (CZ), 14.04.2003-16.04.2003] Grant ostatní: CIAT(XX) SL18 Institutional research plan: CEZ:AV0Z6066911 Keywords : actinomycetes * Colombia * earthworms Subject RIV: EH - Ecology, Behaviour

    1. Investigation of physiologically active products obtained from carbon-ion irradiated actinomycetes

      Charged particles such as carbon-ions are superior to X-rays or gamma-rays in the physical and biological characteristics. The propose research project is aimed to provide new insights on antibiotic development. Carbon-ion exposure reduced cell growth. Product(s) from carbon-ion irradiated microorganera suppressed growth of human leukemia cells. We suggested that carbon-ion irradiated actinomycetes produce antitumor active product(s) for leukemia cells. (author)

    2. Prevalence of viridans streptococci exhibiting lactose-inhibitable coaggregation with oral actinomycetes.

      Kolenbrander, P E; Williams, B L

      1983-01-01

      Fresh oral isolates from human dental plaque were selected on the basis of their spherical morphology. In a double-blind experiment, their species identity and ability to coaggregate with human oral Actinomyces viscosus and Actinomyces naeslundii were determined. Of the 110 isolates characterized, 30 were identified as either Streptococcus mutans, Streptococcus anginosus-constellatus, or Veillonella parvula; none of these coaggregated with the actinomycetes. The remaining 80 isolates were ide...

    3. RESEARCH OF INFLUENCE OF GLYCEROL ON HYDROLYSIS AND FERMENTATION OF MEADOW GRASS BY CELLULOLYTIC ACTINOMYCETES

      Sultanova, L.; Zorin, V.; Petukhova, N.; Sharaeva, A.; Mikhailova, T.; Fedorova, V.

      2011-01-01

      It has been shown that pre-treatment of meadow grass with saline water solutions containing 220 % of glycerol (temperature 120 oC, pressure 98 kPa, time 1 hour) and further washing of lignocelluloses substrate by water lead to significant increase of microorganisms growth, yield of reducing substances and KMC-cellulase activity of extracellular enzymes during the period of cellulolytic actinomycetes cultivation.

    4. Isolation, taxonomy, and antagonistic properties of halophilic actinomycetes in Saharan soils of Algeria.

      Meklat, Atika; Sabaou, Nasserdine; Zitouni, Abdelghani; Mathieu, Florence; Lebrihi, Ahmed

      2011-09-01

      The diversity of a population of 52 halophilic actinomycetes was evaluated by a polyphasic approach, which showed the presence of members of the Actinopolyspora, Nocardiopsis, Saccharomonospora, Streptomonospora, and Saccharopolyspora genera. One strain was considered to be a new member of the last genus, and several other strains seemed to be new species. Furthermore, 50% of strains were active against a broad range of indicators and contained genes encoding polyketide synthetases and nonribosomal peptide synthetases. PMID:21764956

    5. Isolation, Taxonomy, and Antagonistic Properties of Halophilic Actinomycetes in Saharan Soils of Algeria ▿

      Meklat, Atika; Sabaou, Nasserdine; Zitouni, Abdelghani; Mathieu, Florence; Lebrihi, Ahmed

      2011-01-01

      The diversity of a population of 52 halophilic actinomycetes was evaluated by a polyphasic approach, which showed the presence of members of the Actinopolyspora, Nocardiopsis, Saccharomonospora, Streptomonospora, and Saccharopolyspora genera. One strain was considered to be a new member of the last genus, and several other strains seemed to be new species. Furthermore, 50% of strains were active against a broad range of indicators and contained genes encoding polyketide synthetases and nonribosomal peptide synthetases. PMID:21764956

    6. Occupational allergic respiratory diseases in garbage workers: relevance of molds and actinomycetes.

      Hagemeyer, O; Bünger, J; van Kampen, V; Raulf-Heimsoth, M; Drath, C; Merget, R; Brüning, Th; Broding, H C

      2013-01-01

      Exposures to molds and bacteria (especially actinomycetes) at workplaces are common in garbage workers, but allergic respiratory diseases due to these microorganisms have been described rarely. The aim of our study was a detailed analysis of mold or bacteria-associated occupational respiratory diseases in garbage workers. From 2002 to 2011 four cases of occupational respiratory diseases related to garbage handling were identified in our institute (IPA). Hypersensitivity pneumonitis (HP) was diagnosed in three subjects (cases 1-3, one smoker, two non-smokers), occupational asthma (OA) was diagnosed in one subject (case 4, smoker), but could not be excluded completely in case 2. Cases 1 and 2 worked in composting sites, while cases 3 and 4 worked in packaging recycling plants. Exposure periods were 2-4 years. Molds and actinomycetes were identified as allergens in all cases. Specific IgE antibodies to Aspergillus fumigatus were detected exclusively in case 4. Diagnoses of HP were essentially based on symptoms and the detection of specific IgG serum antibodies to molds and actinomycetes. OA was confirmed by bronchial provocation test with Aspergillus fumigatus in case 4. In conclusion, occupational HP and OA due to molds occur rarely in garbage workers. Technical prevention measures are insufficient and the diagnosis of HP is often inconclusive. Therefore, it is recommended to implement the full repertoire of diagnostic tools including bronchoalveolar lavage and high resolution computed tomography in the baseline examination. PMID:23835992

    7. Changes in the structure of the rhizosphere complex of actinomycetes in the ontogenesis of winter rye

      Shirokikh, I. G.; Merzaeva, O. V.; Zenova, G. M.

      2006-06-01

      Changes in the taxonomic structure of actinomycetes in the rhizosphere of winter rye ( Secale cereale L.) growing on acid soddy-podzolic soil were studied. During the first stages of ontogenesis of winter rye, the rhizosphere complex of mycelial prokaryotes was characterized by a relatively level generic structure (with respect to the indices of abundance and frequency of particular genera), low values of the species diversity, and low domination frequency of the species from the Streptomyces genus. The numbers and species diversity of the streptomycetes increased during the further growth of the winter rye, so that streptomycetes became a dominant group in the complex of the rhizosphere actinomycetes. According to the two-way ANOVA, the population density of the Micromonospora and the Streptosporangium genera in the rhizosphere was mainly dictated by the winter rye variety, whereas the population density of the streptomycetes depended on the particular stage of the winter rye development. The differences between the actinomycetal complexes characteristic of different varieties of winter rye at the early stages of its development was leveled by the end of the winter rye growth.

    8. Actinomycete complexes in soils of industrial and residential zones in the city of Kirov

      Shirokikh, I. G.; Solov'eva, E. S.; Ashikhmina, T. Ya.

      2014-02-01

      The number, diversity, and structure of the actinomycetal complexes in the soils of the industrial and residential zones of the city of Kirov are considered. The total content of mobile cadmium, zinc, lead, iron, and nickel in the soils of the industrial biotopes was 1.8 and 6.0 times higher than their concentration in the soils of the residential and background zones, respectively. In the heavy metal (HM)-polluted soils, the share of actinomycetes in the total number of prokaryotes and the relative abundance of the micromono-spores in the actinomycetal complex were much higher and the species diversity of the streptomycetes was lower than these characteristics in the soils of the residential zone. The differences in the composition of the mycelial prokaryote complexes appear to be related to the selective resistance of some of their representatives to heavy metals. The possibility to select the strains resistant to HMs and suitable for use in the bioremediation of polluted soils is considered.

    9. Bioperspective of actinomycetes isolates from coastal soils: A new source of antimicrobial producers

      Rattanaporn Srivibool

      2006-05-01

      Full Text Available Forty five soil samples were collected from four coastal islands on the east coast of Thailand: Chang, Hwai, Lao-yanai in Trat Province and Pai Islands in Chonburi Province. On 3 isolating media, Actinomycetes Isolation Agar, Starch Casein Agar and Glucose Asparagine Agar, 495 isolates of actinomycetes were found. Preliminary test to search for antimicrobial activity was done with Bacillus subtilis TISTR 008, Staphylococcus aureus TISTR 885, Staphylococus aureus TISTR 517 (ATCC 25923, Micrococcus luteus TISTR 884 and Pseudomonas aeruginosa TISTR 781 and Escherichia coli TISTR 887 (ATCC 25922. Fifty-eight actinomycetes were found to be antimicrobial-producing strains. From the morphological determination, cell wall diaminopimelic acid and sugars in whole-cell hydrolysate studies, among the 58 strains, Streptomyces sp. and Actinomadura sp. were the predominant genera. The other antibiotic active strains were Micromonospora sp., Microbispora sp., Nocardia sp., Pseudonocardia sp., Saccharomonospora sp., Streptoalloteichus sp. and Streptoverticillium sp. Most of them could inhibit gram-positive bacteria, especially M. luteus TISTR 884, and 8 strains (4 strains of Actinomadura, 2 strains of Micromonospora, 1 strain of Microbispora, and 1 strain of Streptomyces could inhibit both gram-positive and gram-negative bacteria.

    10. Virus de la Enfermedad Infecciosa de la Bolsa de Fabricio: Relaciones antigénicas de aislados cubanos y chilenos Infectious Bursal Disease Virus: antigenic interrelationships between

      J Noda

      2005-01-01

      Full Text Available La Enfermedad Infecciosa de la Bolsa (EIB sigue afectando la industria avícola por la aparición de variantes patogénicas y antigénicas del virus, originadas en la permanente evolución del virus producto de la presión inmunológica por el uso intensivo de vacunas. La caracterización antigénica de los virus de campo resulta esencial para la aplicación de vacunas más adecuadas en el control de la enfermedad. En este trabajo se determinaron las relaciones antigénicas de aislados cubanos y chilenos, obtenidos de poblaciones de pollos vacunados con el virus de la EIB. Los aislados virales se adaptaron a cultivos celulares y se obtuvieron antisueros monoespecíficos de cada uno de ellos y de una cepa de referencia. Las relaciones se establecieron por virusneu-tralización cruzada utilizando nueve aislados cubanos (BD, BL, 35/95, 29/96, 118/96, BF2, BF8, BF9 y 70/98, tres chilenos (G1, G2 y G4 y una cepa de referencia del serotipo 1 (Lukert. Los aislados cubanos y chilenos se adaptaron eficientemente a cultivos de fibroblastos de embrión de pollo (con excepción de BF3 y G3. Además, los aislados cubanos se adaptaron a células VERO, presentando mayores títulos infectivos en fibroblastos de embrión de pollo que en esta línea celular. Los resultados de la seroneutralización cruzada mostraron entre los aislados cubanos una relación mayor a un 80% y entre éstos y la cepa de referencia mayor de un 70%, de igual modo con los aislados chilenos G1 y G4 (mayor de 77%. El aislado G2 presentó diferencias antigénicas consideradas menores con los aislados cubanos BL, 35/95 y 29/96 (­ 69%. Ninguno de los aislados mostró relaciones antigénicas inferiores al 30% con la cepa de referencia del serotipo 1, por lo tanto no corresponden a cepas variantesInfectious Bursal Disease (IBD is still affecting the poultry industry through the appearance of pathogenic and antigenic variations of the virus. This is due to its permanent evolution as a

    11. Anticancer property of sediment actinomycetes against MCF-7 and MDA-MB-231 cell lines

      Ravikumar S; Fredimoses M; Gnanadesigan M

      2012-01-01

      Objective: To investigate the anticancer property of marine sediment actinomycetes against two different breast cancer cell lines. Methods:In vitro anticancer activity was carried out against breast (MCF-7 and MDA-MB-231) cancer cell lines. Partial sequences of the 16s rRNA gene, phylogenetic tree construction, multiple sequence analysis and secondary structure analysis were also carried out with the actinomycetes isolates. Results: Of the selected five actinomycete isolates, ACT01 and ACT02 showed the IC50 value with (10.13±0.92) and (22.34±5.82)μg/mL concentrations, respectively for MCF-7 cell line at 48 h, but ACT01 showed the minimum (18.54±2.49 μg/mL) level of IC50 value with MDA-MB-231 cell line. Further, the 16s rRNA partial sequences of ACT01, ACT02, ACT03, ACT04 and ACT05 isolates were also deposited in NCBI data bank with the accession numbers of GQ478246, GQ478247, GQ478248, GQ478249 and GQ478250, respectively. The phylogenetic tree analysis showed that, the isolates of ACT02 and ACT03 were represented in group I and III, respectively, but ACT01 and ACT02 were represented in group II. The multiple sequence alignment of the actinomycete isolates showed that, the maximum identical conserved regions were identified with the nucleotide regions of 125 to 221st base pairs, 65 to 119th base pairs and 55, 48 and 31st base pairs. Secondary structure prediction of the 16s rRNA showed that, the maximum free energy was consumed with ACT03 isolate (-45.4 kkal/mol) and the minimum free energy was consumed with ACT04 isolate (-57.6 kkal/mol). Conclusions:The actinomycete isolates of ACT01 and ACT02 (GQ478246 and GQ478247) which are isolated from sediment sample can be further used as anticancer agents against breast cancer cell lines.

    12. The biodegradation of layered silicates under the influence of cyanobacterial-actinomycetes associations

      Ivanova, Ekaterina

      2013-04-01

      The weathering of sheet silicates is well known to be related to local and global geochemical cycles. Content and composition of clay minerals in soil determine the sorption properties of the soil horizons, water-holding capacity of the soil, stickiness, plasticity, etc. Microorganisms have a diverse range of mechanisms of minerals' structure transformation (acid- and alkali formation, biosorption, complexing, etc). One of the methods is an ability of exopolysaccharide-formation, in particular the formation of mucus, common to many bacteria, including cyanobacteria. Mucous covers cyanobacteria are the specific econiches for other bacteria, including actinomycetes. The objective was to analyze the structural changes of clay minerals under the influence of the cyanobacterial-actinomycetes associative growth. The objects of the study were: 1) the experimental symbiotic association, consisting of free-living heterocyst-formative cyanobacterium Anabaena variabilis Kutz. ATCC 294132 and actinomycete Streptomyces cyaneofuscatus FR837630, 2) rock samples obtained from the Museum of the Soil Science Department of the Lomonosov Moscow State University: kaolinite, consisting of kaolin (96%) Al4 (OH) 8 [Si4O10]; mixed with hydromica, chlorite and quartz; vermiculite, consisting of vermiculite (Ca, Mg, ...)*(Mg, Fe)3(OH)2[(Si, Al)4O10]*4H2O and trioctahedral mica (biotite). The mineralogical compositions of the rocks were determined by the universal X-ray Diffractometer Carl Zeiss Yena. The operationg regime was kept constant (30 kv, 40 mA). The cultivation of the association of actinomycete S. cyanoefuscatus and cyanobacterium A. variabilis caused a reduction in the intensity of kaolinite and hydromica reflexes. However, since both (mica and kaolinite) components have a rigid structure, the significant structural transformation of the minerals was not revealed. Another pattern was observed in the experiment, where the rock sample of vermiculite was used as the mineral

    13. Estudio anatomopatológico de aislados de Leptospira spp., provenientes de Nicaragua en Mesocricetus auratus como biomodelo

      Willian Jirón T.

      2013-08-01

      Full Text Available Objetivo. El objetivo de este trabajo fue caracterizar en el biomodelo Mesocricetus auratus la sintomatología y lesiones anatomopatológicas que provocan 5 aislados clínicos de Leptospira spp., provenientes de Nicaragua. Materiales y métodos. Con este fin se inocularon 50 hámster por vía i.p con 1mL del cultivo de cada una de las cepas en fase exponencial teniendo una concentración celular de 7.5 x 106 leptospira/mL (10 animales por cepa, evaluándose signos de la enfermedad, mortalidad durante 14 días, lesiones anatomopatológicas macroscópicas y microscópicas mediante tinción con hematoxilina-eosina y tinción de Warthyn Starryn. Resultados. Todas las cepas presentaron alta mortalidad, mostrando un cuadro tanto clínico, como lesional característico de la infección experimental. Además, causaron la muerte al 100% de los animales entre el tercer y décimo día postinfección. En el estudio anatomopatológico la cepa del serogrupo Ballum y la del serogrupo Pomona produjeron focos de hemorragias específicamente en el riñón y pulmones. De forma similar ocurrió una congestión hepática y renal, mientras que la hemorragia renal fue observada con mayor frecuencia en la cepa del serogrupo Pomona, diferenciándose del resto de las cepas que mostraron esta lesión con menos frecuencia. Conclusiones. Este trabajo permitió una mayor caracterización de estas cepas siendo utilizadas como futuras candidatas vacunales frente a una nueva epidemia de Leptospirosis en Nicaragua.

    14. Gérmenes patógenos aislados en niños con infecciones respiratorias a repetición

      Yoenny Peña García

      2015-01-01

      Full Text Available Las infecciones respiratorias agudas son la causa más frecuente de morbilidad y de elevada mortalidad en el mundo, particularmente en los países en desarrollo. Se realizó un estudio descriptivo de corte transversal, para caracterizar a los niños con infecciones respiratorias a repetición, según los gérmenes patógenos aislados, en el círculo infantil “Flores de la Vida” del municipio de Puerto Padre, en el período de abril a mayo de 2014. Se trabajó con los niños de los salones de segundo, tercero y cuarto año de vida, distribuidos según edad y sexo, a los que se les identificaron los gérmenes patógenos aislados en exudados nasofaríngeos. La información se obtuvo del departamento de microbiología del Centro Municipal de Higiene y Epidemiología. Se obtuvieron los siguientes resultados: el grupo de edad más frecuente fue el de dos años, que representó el 45 % de la muestra y se correspondió con la media de la edad. El 62,5 % de la muestra de estudio correspondió al sexo masculino. Los gérmenes patógenos aislados con mayor frecuencia fueron: el Streptococcus pneumoneae, para un 83,9 %, el Streptococcus B hemolítico, para un 7,1 % y el Haemophillus inflienzae para un 5,4 %

    15. Succession of Actinomycetes During Composting Proccess of Dairy-Farm Waste Investigated by Culture-Dependent and Independent Approaches

      Mukhlissul Faatih1

      2015-11-01

      Full Text Available Mesophilic, thermophilic, and maturation phases were recognized in composting proccess. Temperaturechanges influence the microbial communities in compost within composting proccess. Actinomycetes account for alarger part of compost microbial population. The aim of this research was to study succession of actinomycetescommunity during composting of dairy-farm waste investigated by culture-dependent and independentapproaches.In culture-independent method, the succession of actinomycetes community was analyzed by nestedpolymerasechain reaction of ribosomal intergenic spacer (nested-PCR RISA using spesific primer F243 and primerR23S followed by a second PCR using primers F968 and R23S. In culture-dependent method actinomycetes fromcompost were isolated on selective media, starch-nitrate medium and humic-acid + vitamins medium. DNA ofactinomycetes was extracted and amplified by repetitive sequence-based PCR (rep-PCR using primer BOXA1R. Thebanding patterns were used to generate dendrograms by UPGMA clustering with NTSYS program. Microcosmcontaining sterile rice-straw and water which is inoculated with each actinomycetes isolates was used for examiningthe ability of each isolate in rice-straw degradation.The experiment results showed that succession of both bacteria and actinomycetes was occured withincomposting proccess of dairy-farm waste. Analysed by culture-independent method revealed that the highestcommunity of compost’s bacteria was on mesophilic, thermophilic, and maturation phases, respectively. WhereasPCR-nested RISA resulted the highest population of actinomycetes was on thermophilic, maturation, and mesophilicphases, respectively. By culture-dependent method was obtained 29 actinomycetes isolates from mesophilic phase,23 isolates from thermophilic phase, and 19 isolates from maturation phase. Genetic diversity analysis of the obtainedisolates showed the presence of phylogenetic grouping on each phase of composting proccess. This result

    16. Virus de la Enfermedad Infecciosa de la Bolsa de Fabricio: Relaciones antigénicas de aislados cubanos y chilenos Infectious Bursal Disease Virus: antigenic interrelationships between

      J Noda; J ULLOA; C L Perera; G Jara; Cuello, S.; E. Rodríguez

      2005-01-01

      La Enfermedad Infecciosa de la Bolsa (EIB) sigue afectando la industria avícola por la aparición de variantes patogénicas y antigénicas del virus, originadas en la permanente evolución del virus producto de la presión inmunológica por el uso intensivo de vacunas. La caracterización antigénica de los virus de campo resulta esencial para la aplicación de vacunas más adecuadas en el control de la enfermedad. En este trabajo se determinaron las relaciones antigénicas de aislados cubanos y chileno...

    17. Selección de hongos aislados de bagazo de caña con actividad celulasa sobre celulosa cristalina para posibles aplicaciones industriales

      Yoandy Ferrer-Marcelo; Marta León-Rodríguez; Georgina Michelena-Álvarez; Julio César Dustet-Mendoza; Arianna Duque-Ortiz; My-Lai Ibañez-Fuentes; Keyla Tortoló-Cabañas

      2011-01-01

      Las enzimas comerciales necesarias en procesos de producción de bioetanol celulósico y enriquecimiento de forrajes, son excesivamente caras. La mayoría provienen de mutantes B-glucosidasa, con la velocidad y extensión de la hidrólisis reducida, y débil acción sobre la celulosa cristalina. A partir de bagazo, se aislaron varios hongos filamentosos con capacidad de degradación de la celulosa cristalina, para ello se diseñó un medio selectivo de celulosa agar. Del total de aislados, solo 9 micro...

    18. Variabilidad morfológica, patogénica y susceptibilidad a fungicidas de Rhizoctonia solani aislado de rizósfera de Vitis vinifera var. perlette seedless

      Amparo Meza-Moller; Martín Esqueda; Alfonso Gardea; Martín Tiznado; Gil Virgen-Calleros

      2007-01-01

      Se estudió la variabilidad morfológica, reacción de anastomosis, patogenicidad y susceptibilidad a fungicidas de Rhizoctonia solani aislado de la rizósfera de Vitis vinifera var. perlette seedless plantada a pie franco en Sonora, México. El hongo se aisló mediante el método de tamizado en húmedo y la siembra en agar-agua 2 %. Se obtuvieron 122 cepas, las cuales presentaron hifas con 3 a 9 núcleos por célula, de 3 a 8 µm de diámetro y de color blanco sucio a moreno oscuro. Los aislamientos cor...

    19. Actividad antifúngica del aceite esencial de Eugenia caryophyllata sobre cepas de Candida tropicalis de aislados clínicos

      Juliana MOURA MENDES; Felipe Queiroga SARMENTO GUERRA; de Oliveira Pereira, Fillipe; Janiere PEREIRA de SOUSA; Vinicius NOGUEIRA TRAJANO; de Oliveira Lima, Edeltrudes

      2012-01-01

      La candidiasis es una infección fúngica oportunista causada por levaduras del género Candida. En Brasil, la especie C. tropicalis esta siendo aislada frecuentemente, es el segundo microorganismo más aislado después de C. albicans. La aparición de cepas resistentes a los antifúngicos convencionales ha aumentado la búsqueda de nuevas alternativas provenientes de productos naturales, especialmente los aceites esenciales. En este estudio se investigó la actividad de los aceites esenciales contra ...

    20. Detección de aislados clínicos de Escherichia coli y Klebsiella spp. productoras de B-lactamasas de espectro extendido mediante el sistema DIRAMIC

      Estrella Álvarez-Varela

      2010-01-01

      Full Text Available En los últimos años, se ha incrementado el interés por las enterobacterias productoras de B-lactamasas de espectro extendido (BLEE. Las cepas que expresan este fenotipo son capaces de hidrolizar un amplio grupo de antibióticos, incluidas las cefalosporinas de tercera generación y aztreonam, por lo que constituyen un reto terapéutico de difícil abordaje. Este mecanismo les confiere a las bacterias un grado de resistencia variable a los oximino-betalactámicos, se manifiesta en un aumento de la concentración mínima inhibitoria, que permanece en el intervalo de sensibilidad. Puede pasar inadvertido en el laboratorio, pero se manifiesta en el paciente, en el que no hay actividad de la droga y por lo tanto, se presenta una falla terapéutica. De ahí, la gran importancia de perfeccionar su detección a nivel del laboratorio de microbiología. En este trabajo, se comparó el sistema DIRAMIC con el método de sinergia de doble disco modificado (DDD para la detección fenotípica de BLEE. Se estudiaron 175 aislados clínicos, 132 Escherichia coli y 43 Klebsiella spp., provenientes de tres hospitales de Ciudad de La Habana, recolectados de junio de 2008 a junio de 2009. De un total de 42 (24 % aislados que presentaron fenotipo de BLEE por el método de DDD, el sistema DIRAMIC fue capaz de detectar 39 (22,3 %. En tres aislados, el fenotipo de BLEE solo fue detectado por DDD, los cuales resultaron falsos negativos por DIRAMIC. El 72,6 % de los aislados (127 fueron negativos por ambos métodos. Los métodos empleados tuvieron un desempeño similar, para un 92,85 % de correspondencia. El DIRAMIC mostró una sensibilidad del 92,85 % y una especificidad del 95,48 % con respecto al método de DDD.

    1. SELEKSI DAN PEMANFAATAN ACTINOMYCETES SEBAGAI MIKROBA ANTAGONIS YANG RAMAH LINGKUNGAN TERHADAP Fusarium oxysporum f.sp. cubense SECARA IN VITRO

      I MADE SUDARMA

      2015-06-01

      Full Text Available A total of 119 different actinomycete isolate were recovered from banana crop habitats with and without Fusarium wilt disease symptom. These were than assessed for their antagonist ability against Fusarium oxysporum £sp. cubense (Foe in vitro. Results indicated that four of all actinomycete isolate active against Foe. The four of actinomycete isolates were Streptomyces sp. l (AAo4, Streptomyces sp.2 (AAo32 , Streptomyces sp.3 (AAo33 and Streptomyces sp. 4 (AAo35. It was can inhibit the Foe mycelium growth, 79,63%, 72,22%, 78,89% and 72,22% respectively. After tested with the 3 times replication, the four Streptomyces spp. isolate effective to control the Foe that attack Bali banana cultivars, such as Susu, Saba, Raja and Ketip.

    2. Lignin-solubilizing ability of actinomycetes isolated from termite (Termitidae) gut

      The lignocellulose-degrading abilities of 11 novel actinomycete strains isolated from termite gut were determined and compared with that of the well-characterized actinomycete, Streptomyces viridosporus T7A. Lignocellulose bioconversion was followed by (i) monitoring the degradation of [14C]lignin- and [14C]cellulose-labeled phloem of Abies concolor to 14CO2 and 14C-labeled water-soluble products, (ii) determining lignocellulose, lignin, and carbohydrate losses resulting from growth on a lignocellulose substrate prepared from corn stalks (Zea mays), and (iii) quantifying production of a water-soluble lignin degradation intermediate (acid-precipitable polymeric lignin). Of the assays used, total lignocellulose weight loss was most useful in determining overall bioconversion ability but not in identifying the best lignin-solubilizing strains. A screening procedure based on 14CO2 evolution from [14C-lignin]lignocellulose combined with measurement of acid-precipitable polymeric lignin yield was the most effective in identifying lignin-solubilizing strains. For the termite gut strains, the pH of the medium showed no increase after 3 weeks of growth on lignocellulose. This is markedly different from the pattern observed with S. viridosporus T7A, which raises the medium pH considerably. Production of extracellular peroxidases by the 11 strains and S. viridosporus T7A was followed for 5 days in liquid cultures. On the basis of an increase of specific peroxidase activity in the presence of lignocellulose in the medium, the actinomycetes could be placed into the same three groups

    3. Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia

      Gebreselema Gebreyohannes; Feleke Moges; Samuel Sahile; Nagappan Raja

      2013-01-01

      To isolate, evaluate and characterize potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia. Methods: A total of 31 strains of actinomycetes were isolated and tested against Gram positive and Gram negative bacterial strains by primary screening. In the primary screening, 11 promising isolates were identified and subjected to solid state and submerged state fermentation methods to produce crude extracts. The fermented biomass was extracted by organic solvent extraction method and tested against bacterial strains by disc and agar well diffusion methods. The isolates were characterized by using morphological, physiological and biochemical methods. Results: The result obtained from agar well diffusion method was better than disc diffusion method. The crude extract showed higher inhibition zone against Gram positive bacteria than Gram negative bacteria. One-way analysis of variance confirmed most of the crude extracts were statistically significant at 95% confidence interval. The minimum inhibitory concentration and minimum bactericidal concentration of crude extracts were 1.65 mg/mL and 3.30 mg/mL against Staphylococcus aureus, and 1.84 mg/mL and 3.80 mg/mL against Escherichia coli respectively. The growth of aerial and substrate mycelium varied in different culture media used. Most of the isolates were able to hydrolysis starch and urea; able to survive at 5% concentration of sodium chloride; optimum temperature for their growth was 30 °C. Conclusions: The results of the present study revealed that freshwater actinomycetes of Lake Tana appear to have immense potential as a source of antibacterial compounds.

    4. Purification and characterization of protease enzyme from actinomycetes and its cytotoxic effect on cancer cell line (A549)

      C Balachandran; V Duraipandiyan; S Ignacimuthu

      2012-01-01

      Objective: To isolate active actinomycetes from soil samples of Northern Himalayas and study their culture characterization, protease production and cytotoxic effects on cancer cell line (A549). Methods: Forty six strains of actinomycetes were isolated from the soil collected from Northern Himalayas, India. Isolation of actinomycetes was performed by serial dilution plate technique. Forty six isolated actinomycetes cultures were grown in ISP 2 medium to study the morphology and biochemical characteristics. Isolated strains were studied for protease enzyme production in skim milk agar medium with solubilising capacity. Seven isolates were studied for melanin pigmentation and different NaCl concentration. Effects of environmental conditions influencing protease enzyme production of seven isolated strains were also studied at different pH, temperature and metal ions (β-mercaptoethanol, dithiothreitol, iodoacetamide, MgSO4, CaCl2 and EDTA). The seven isolates were also studied for lytic enzyme activity using different bacteria and yeast such as Pseudomonas aeruginosa (P. aeruginosa), Enterococcus feacalis (E. feacalis), Escherishia coli (E. coli), Candida albicans (C. albicans), Bacillus subtilis (B. subtilis), Klebsiella pneumonia (K. pneumonia) and Staphylococcus aureus (S. aureus). Results: Isolates ERIA-31 and ERIA-33 produced more protease enzyme activity in modified nutrient agar media compared to other actinomycetes cultures. ERIA-31 and ERIA-33 were tested for cytotoxic effect in human adenocarcinoma cancer cell line (A549). IC50 for ERIA-31 was 57.04 μg/mL and IC50 for ERIA-33 was 55.07 μg/mL. Conclusion: Actinomycete being a protease producing bacteria has the potential for use in industrial purpose, pharmaceuticals, cytotoxic agent and its proteolytic activity. Isolates of ERIA-31 and ERIA-33 produced significant amount of protease enzymes.

    5. Investigation of physiologically active products obtained from carbon-ion irradiated actinomycetes

      Charged particles such as carbon-ions are superior to X-rays or gamma-rays in the physical and biological characteristics. The propose research project is aimed to provide new insights on antibiotic development. Mutants were prepared by heavy ion irradiation, examined the effect of physiologically active substances produced. Product(s) from carbon-ion irradiated microorganera suppressed growth of human cololectal cancer cells and breast cancer cells. We suggested that carbon-ion irradiated actinomycetes produce antitumor active product(s) for cololectal and breast cancer cells. (author)

    6. Investigation of physiologically active products obtained from carbon-ion irradiated actinomycetes

      Charged particles such as carbon-ions are superior to X-rays or gamma-rays in the physical and biological characteristics. The propose research project is aimed to provide new insights on antibiotic development. Product(s) from carbon-ion irradiated microorganera suppressed growth of human leukemia cells and mammary tumor cells. This product(s) induced apoptosis in human leukemia cells. We suggested that carbon-ion irradiated actinomycetes produce antitumor active product(s) for leukemia cells and mammary tumor cells. (author)

    7. Application of TLC in the Screening of Acarbose-producing Actinomycetes

      Fei Ren; Long Chen; Qunyi Tong

      2014-01-01

      Acarbose is widely used in medicine, such as the treatment of diabetes and obesity. A simple Thin Layer Chromatography (TLC)-scanning technique was developed for the rapid and accurate analysis of acarbose in a large number of fermentation broths of actinomycetes to screen for acarbose producer. The linearity of the acarbose in this way was good within the range from 2 to 10 μg (r2 = 0.9997). This technique didn’t need expensive instrument and complex procedure for the detection of acarbose i...

    8. EXPLORATION OF ACTINOMYCETES ENDOPHYTICALLY ASSOCIATED WITH PIPER NIGRUM FOR POTENTIAL BIOACTIVITY

      Jasim B.

      2015-02-01

      Full Text Available Piper nigrum is well known for its metabolite richness. So endophytic microorganisms that reside within such environments can be expected to have promising biosynthetic potential. The current study identified three endophytic actinomycetes with broad bioactivity which can have applications in natural product related pharmacological research. The Verrucosispora sp identified in the study was found to have promising anticancer and antimicrobial activities and Streptomyces sp. was found to have antioxidant activity. The results obtained are supported by many previous reports and this suggests the isolates obtained in the study to have the possible presence of potential known or novel compounds with broad spectrum of activity.

    9. II. Derecho desde arriba y desde abajo: Physis y Nomos69

      Rubio, Mauricio

      2014-01-01

      El ancestral debate entre las dos maneras de ver el mundo, desde arriba o desde abajo, ha tomado en la filosofía y el derecho la forma de dos conceptos paralelos, la naturaleza y la ley, physis y nomos. Desde Platón y Epicúreo, o la filosofía y la retórica entre los griegos, hasta el naturalismo y el historicismo, pasando por el conflicto entre la escolástica y el humanismo en el Renacimiento, se pueden distinguir dos visiones antagónicas, la una centrada en el universo, su estructura y sus t...

    10. Genome sequencing reveals complex secondary metabolome in themarine actinomycete Salinispora tropica

      Udwary, Daniel W.; Zeigler, Lisa; Asolkar, Ratnakar; Singan,Vasanth; Lapidus, Alla; Fenical, William; Jensen, Paul R.; Moore, BradleyS.

      2007-05-01

      Recent fermentation studies have identified actinomycetes ofthe marine-dwelling genus Salinispora as prolific natural productproducers. To further evaluate their biosynthetic potential, we analyzedall identifiable secondary natural product gene clusters from therecently sequenced 5,184,724 bp S. tropica CNB-440 circular genome. Ouranalysis shows that biosynthetic potential meets or exceeds that shown byprevious Streptomyces genome sequences as well as other naturalproduct-producing actinomycetes. The S. tropica genome features ninepolyketide synthase systems of every known formally classified family,non-ribosomal peptide synthetases and several hybrid clusters. While afew clusters appear to encode molecules previously identified inStreptomyces species,the majority of the 15 biosynthetic loci are novel.Specific chemical information about putative and observed natural productmolecules is presented and discussed. In addition, our bioinformaticanalysis was critical for the structure elucidation of the novelpolyenemacrolactam salinilactam A. This study demonstrates the potentialfor genomic analysis to complement and strengthen traditional naturalproduct isolation studies and firmly establishes the genus Salinispora asa rich source of novel drug-like molecules.

    11. Antimicrobial potential of Halophilic actinomycetes against multi drug resistant (MDR) ventilator associated pneumonia causing bacterial pathogens.

      Aslam, Sana; Sajid, Imran

      2016-03-01

      A collection of forty halophilic actinomycetes isolated from water and mud samples of the saline lake at Kalar Kahar, salt range, Pakistan, was screened to investigate their antimicrobial potential against multi drug resistant (MDR) ventilator associated pneumonia causing bacterial pathogens. The isolates exhibited significant tolerance to alkaline conditions and grew well at pH 9-11. The taxonomic status of the isolated strains was determined by morphological, biochemical and physiological characterization and by 16s rRNA gene sequencing. The results revealed that majority of the isolates (90%) belong to the genus Streptomyces. Most of the isolates exhibited remarkable antimicrobial activity up to 20mm zone of inhibition against MDR ventilator associated pneumonia causing bacteria including Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumoniae, Escherichia coli, Enterobacter and Acinetobacter spp. Additionally the isolates showed moderate to high cytotoxicity in the range of 40 to 80% larval mortality against Artemia salina in a micro well cytotoxicity assay. The chemical screening or the so called metabolic fingerprinting of the methanolic extracts of each isolate, by thin layer chromatography (TLC) using various staining reagents and by high performance liquid chromatography (HPLC-UV), indicated an impressive diversity of the compounds produced by these strains. The study reveals that these halophilic actinomycetes are a promising source of bioactive compounds. The preparative scale fermentation, isolation, purification and structure elucidation of the compounds produced by them may yield novel antimicrobial or chemotherapeutic agents. PMID:27087086

    12. Viability of fungal and actinomycetal spores after microwave radiation of building materials.

      Górny, Rafał L; Mainelis, Gediminas; Wlazło, Agnieszka; Niesler, Anna; Lis, Danuta O; Marzec, Stanisław; Siwińska, Ewa; Łudzeń-Izbińska, Beata; Harkawy, Aleksander; Kasznia-Kocot, Joanna

      2007-01-01

      The effects of microwave radiation on viability of fungal and actinomycetal spores growing on agar (medium optimal for growth) as well as on wooden panel and drywall (common building construction/finishing materials) were studied. All materials were incubated at high (97-99%) and low (32-33%) relative humidity to mimic "wet" and "dry" environmental conditions. Two microwave power densities (10 and 60 mW/cm2) and three times of exposure (5, 30, and 60 min) were tested to find the most effective parameters of radiation which could be applied to non-invasive reduction or cleaning of building materials from microbial contaminants. Additionally, a control of the surface temperature during the experiments allowed differentiation between thermal and microwave effect of such radiation. The results showed that the viability of studied microorganisms differed depending on their strains, growth conditions, power density of microwave radiation, time of exposure, and varied according to the applied combination of the two latter elements. The effect of radiation resulting in a decrease of spore viability on "wet" wooden panel and drywall was generally observed at 60 min exposure. Shorter exposure times decreased the viability of fungal spores only, while in actinomycetes colonizing the studied building materials, such radiation caused an opposite (supporting growth) effect. PMID:18247470

    13. Antimethicilin resistance agents from marine actinomycetes from soil sediments of Lagos Lagoon

      Davies Olabisi Flora; Adeleye Isaac Adeyemi; Wang Peng George

      2015-01-01

      Objective:To evaluate theisolation of actinomycetes strains with potential for producing antimicrobials with high methicilin resistance capability. Methods: The soil samples were collected from four different locations of Lagos lagoon. TheActinomycetes were isolated from the samples by serial dilution using spread plate method. Isolates were selected based on their cultural characteristics as well as their Gram reaction and phenotypically and molecularly characterizedStreptomyces sp. Isolates were inoculated in starch casein and Kuster’s broth media and secondary metabolites were screened for antimicrobial activity against the following microorganisms: methicillin resistant Staphylococcus aureus,Staphylococcus aureusATCC29213,Escherichia coliATCC 29522, Pseudomonas aeruginosaATCC27853,Candida albicans,Enterococcus faecalisATCC 29212. Coagulase-negative staphylococci isolated fromHIV patients were also used (Staphylococcus warneri,Staphylococcus xylosus andStaphylococcus epidermidis). The antimicrobial metabolites of the isolates were identified using gas chromatography-mass spectrometer. Results:Extracts from isolatesULS12 andULS13 showed antimicrobial activity against methicillin resistantStaphylococcus aureus whileULK3 inhibitedCandida albicans only. The gas chromatography-mass spectrometerdata analysis showed the antibiotic profile of these isolates. Conclusions: The isolatesULS12 andULS13 were found to display the highest antimicrobial activity against the test organisms and could be a potential source of new antibiotics.

    14. Endophytic Actinomycetes: A Novel Source of Potential Acyl Homoserine Lactone Degrading Enzymes

      Surang Chankhamhaengdecha

      2013-01-01

      Full Text Available Several Gram-negative pathogenic bacteria employ N-acyl-L-homoserine lactone (HSL quorum sensing (QS system to control their virulence traits. Degradation of acyl-HSL signal molecules by quorum quenching enzyme (QQE results in a loss of pathogenicity in QS-dependent organisms. The QQE activity of actinomycetes in rhizospheric soil and inside plant tissue was explored in order to obtain novel strains with high HSL-degrading activity. Among 344 rhizospheric and 132 endophytic isolates, 127 (36.9% and 68 (51.5% of them, respectively, possessed the QQE activity. The highest HSL-degrading activity was at 151.30±3.1 nmole/h/mL from an endophytic actinomycetes isolate, LPC029. The isolate was identified as Streptomyces based on 16S  rRNA gene sequence similarity. The QQE from LPC029 revealed HSL-acylase activity that was able to cleave an amide bond of acyl-side chain in HSL substrate as determined by HPLC. LPC029 HSL-acylase showed broad substrate specificity from C6- to C12-HSL in which C10HSL is the most favorable substrate for this enzyme. In an in vitro pathogenicity assay, the partially purified HSL-acylase efficiently suppressed soft rot of potato caused by Pectobacterium carotovorum ssp. carotovorum as demonstrated. To our knowledge, this is the first report of HSL-acylase activity derived from an endophytic Streptomyces.

    15. Development of actinomycetes in brown semidesert soil under low water pressure

      Zvyagintsev, D. G.; Zenova, G. M.; Sudnitsyn, I. I.; Gracheva, T. A.; Lapygina, E. E.; Napol'skaya, K. R.; Sydnitsyna, A. E.

      2012-07-01

      Under laboratory conditions, the spores of a xerotolerant Streptomyces odorifera strain germinated in brown semidesert soil even at extremely low soil water pressure ( P = -96.4 MPa, -964 atm, a w 0.50); the plantlets increased in length and formed mycelium, on which a new generation of spores was produced (a complete development cycle of the actinomycetes—from a spore to the formation of new spores—passed). The duration of the first cycles of the actinomycetes' development varied from 13 days at P = -27 atm to 57 days at P = -964 atm and was directly proportional to the absolute value of the soil water pressure ( P). In the first cycles of the actinomycetes' development, the rate of increase of the concentration of the germinated spores and mycelium, as well as the logarithms of the mycelium-to-germinated spore concentration ratios, was inversely proportional to the logarithm of P. These relationships indicated that the energy state of the water determined its availability to soil biota and, hence, the activity of its physiological and biochemical processes.

    16. Phosphatic precipitates associated with actinomycetes in speleothems from Grand Cayman, British West Indies

      Jones, Brian

      2009-07-01

      Calcitic speleothems from a cave located on the north central coast of Grand Cayman commonly include corrosion surfaces that developed when calcite precipitation ceased and corrosion mediated by condensates became the operative process. Dissolution features associated with these surfaces, including etched crystal surfaces, microcavities, and solution-widened boundaries between crystals, are commonly occupied by microbes and microbial mats that have been replaced by calcium phosphate and/or coated with calcium phosphate. No mineralized microbes were found in the calcite crystals that form the speleothems. The morphology of the mineralized hyphae (eight morphotypes) and spores (nine morphotypes) are indicative of actinomycetes, a group of microbes that are ideally adapted to life in oligotrophic cave environs. Superb preservation of the delicate hyphae, aerial hyphae, and delicate ornamentation on the hyphae and spores indicate that the microbes underwent rapid mineralized while close to their original life positions. Although these actinomycetes were extremely susceptible to replacement by calcium phosphate, there is no evidence that they directly or indirectly controlled precipitation. Nevertheless, the association between the P-rich precipitates and microbes shows that the use of phosphorus as a proxy for seasonal climate changes in paleoclimate analyses must be treated with caution.

    17. Structural and functional properties of actinomycetal communities in chernozems and saline soils of Ukraine

      Grishko, V. N.; Syshchikova, O. V.

      2010-02-01

      In the profiles of ordinary and southern chernozems, the total numbers of amylolytic microorganisms and actinomycetes decreased with the depth by 2.4-4.2 and 3.4 times, respectively; in the profiles of solonetz and solonchak soils, by 4.2-5.3 and 4.8 times, respectively. In the genetic horizons of the ordinary and southern chernozems, the share of actinomycetes amounted to 29-30% of the total population of microorganisms; in the saline soils, it increases with the depth from 23 to 43%. In the chernozems, Streptomyces violaceomaculatus (Roseus section), St. sporoherbeus (Azureus), St. aerionidulus (Cinereus), St. enduracidicus (Cinereus), and St. grisinus (Cinereus) predominated; in the saline soils, St. violaceomaculatus and St. aerionidulus prevailed. In the ordinary chernozem, the Berger-Parker index was 1.5 times higher than in the southern chernozem. High similarity was found between the streptomycete communities in the chernozems (the Sorensen coefficient was 0.78). In the solonetzes, the species richness of the streptomycetes was higher by 1.7 times than in the solonchaks. In the chernozems, the similarity of the streptomycete communities was higher than in the solonchaks (0.78 and 0.60, respectively).

    18. Screening of Marine Actinomycetes from Segara Anakan for Natural Pigment and Hydrolytic Activities

      Asnani, A.; Ryandini, D.; Suwandri

      2016-02-01

      Marine actinomycetes have become sources of great interest to natural product chemistry due to their new chemical entities and bioactive metabolites. Since April 2010, we have screened actinobacteria from five sites that represent different ecosystems of Segara Anakan lagoon. In this present study we focus on specific isolates, K-2C which covers 1) actinomycetes identification based on morphology observation and 16S rRNA gene; 2) fermentation and isolation of pigment; 3) structure determination of pigment; and 4) hydrolytic enzymes characterization; Methodologies relevant to the studies were implemented accordingly. The results indicated that K-2C was likely Streptomyces fradiae strain RSU15, and the best fermentation medium should contain starch and casein with 21 days of incubation. The isolate has extracellular as well as intracellular pigments. Isolated pigments gave purple color with λmax of 529.00 nm. The pigment was structurally characterized. Interestingly, Streptomyces K-2C was able to produce potential hydrolytic enzymes such as amylase, cellulase, protease, lipase, urease, and nitrate reductase.

    19. Antimethicilin resistance agents from marine actinomycetes from soil sediments of Lagos Lagoon

      Davies Olabisi Flora

      2015-03-01

      Full Text Available Objective: To evaluate the isolation of actinomycetes strains with potential for producing antimicrobials with high methicilin resistance capability. Methods: The soil samples were collected from four different locations of Lagos lagoon. The Actinomycetes were isolated from the samples by serial dilution using spread plate method. Isolates were selected based on their cultural characteristics as well as their Gram reaction and phenotypically and molecularly characterized Streptomyces sp. Isolates were inoculated in starch casein and Kuster’s broth media and secondary metabolites were screened for antimicrobial activity against the following microorganisms: methicillin resistant Staphylococcus aureus, Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 29522, Pseudomonas aeruginosa ATCC 27853, Candida albicans, Enterococcus faecalis ATCC 29212. Coagulase-negative staphylococci isolated from HIV patients were also used (Staphylococcus warneri, Staphylococcus xylosus and Staphylococcus epidermidis. The antimicrobial metabolites of the isolates were identified using gas chromatography-mass spectrometer. Results: Extracts from isolates ULS12 and ULS13 showed antimicrobial activity against methicillin resistant Staphylococcus aureus while ULK3 inhibited Candida albicans only. The gas chromatography-mass spectrometer data analysis showed the antibiotic profile of these isolates. Conclusions: The isolates ULS12 and ULS13 were found to display the highest antimicrobial activity against the test organisms and could be a potential source of new antibiotics.

    20. Azalomycin F4a 2-ethylpentyl ester, a new macrocyclic lactone, from mangrove actinomycete Streptomyces sp.211726

      Gan Jun Yuan; Kui Hong; Hai Peng Lin; Jia Li

      2010-01-01

      Azalomycin F4a 2-ethylpentyl ester,a new 36-membered macrocyclic lactone antibiotic,was isolated from mangrove actinomycete Streptomyces sp.211726.Its structure was elucidated on the basis of spectroscopic data.The compound showed broad-spectrum antifungal activity and moderate cytotoxicity against human colon tumor cell HCT-116.

    1. Distribution and generic composition of culturable marine actinomycetes from the sediments of Indian continental slope of Bay of Bengal

      Das, Surajit; Lyla, P. S.; Ajmal Khan, S.

      2008-05-01

      Actinomycetes population from continental slope sediment of the Bay of Bengal was studied. Samples were collected during two voyages of FORV Sagar Sampada in 2004 (May-June) and 2005 (July) respectively from 11 transects (each transect had ca. 200 m, 500 m, and 1 000 m depth stations). The physicochemical parameters of overlying water, and sediment samples were also recorded. The actinomycete population ranged from 5.17 to 51.94 CFU/g dry sediment weight and 9.38 to 45.22 CFU/g dry sediment weight during the two cruises respectively. No actinomycete colony was isolated from stations in 1 000 m depth. Two-way analysis of variance showed significant variation among stations (ANOVA two-way, P0.05). Three actinomycetes genera were identified. Streptomyces was found to be the dominating one in both the cruises, followed by Micromonospora, and Actinomyces. The spore of Streptomyces isolates showed the abundance in spiral spore chain. Spore surface was smooth. Multiple regression analysis revealed that the influencing physico-chemical factors were sediment pH, sediment temperature, TOC, porosity, salinity, and pressure. The media used in the present study was prepared with seawater. Thus, they may represent an autochthonous marine flora and deny the theory of land runoff carriage into the sea for adaptation to the salinity of the seawater and sediments.

    2. Isolation, screening and characterization of Uranium microremediable actinomycetes from fallen leaves of Azadirachta indica in Western Ghats

      The aim of this study was to isolate and screen the actinomycetes from fallen leaves of Azadirachta indica (Neem) in Western Ghats, India that has the capacity to microremediate radioactive waste Uranium. The influence of different temperature and pH on Uranium removal was also studied

    3. In vitro Antimicrobial Assay of Actinomycetes in Rice AgainstXanthomonas oryzae pv. oryzicola and as Potential Plant Growth Promoter

      Erneeza Mohd Hata

      2015-12-01

      Full Text Available ABSTRACT The aim of this work was to invitro assay the antimicrobial activity of actinomycetes in rice against Xanthomonas oryzae pv. oryzicola and as potential plant growth promoter. A total of 92 actinomycete strains were isolated from different rice plant components and field locations. Of these, only 21.74% showed antagonistic activity against the Xoc pathogen. Molecular identification via 16s rRNA amplification revealed that 60% of the active antagonistic strains belonged to the genus Streptomyces. Isolates that demonstrated the highest antagonistic activity were also able to produce hydrolytic enzymes and plant growth-promoting hormones. Combination of preliminary screening based on in vitro antagonistic, hydrolytic enzyme and plant growth hormone activity facilitated the best selection of actinomycete candidates as evidenced by strains classification using cluster analysis (Ward's Method. Results from the preliminary screening showed that actinomycetes, especially Streptomycetes, could offer a promising source for both biocontrol and plant growth-promotion agents against BLS disease in rice.

    4. Resistencia y susceptibilidad de microorganismos aislados en pacientes atendidos en una institución hospitalaria de tercer nivel, Villavicencio-Colombia, 2012

      Oscar Alexander Gutiérrez Lesmes

      2015-05-01

      Full Text Available Introducción: La resistencia bacteriana es problema significativo de salud pública y está asociada al uso indiscriminado e irracional de antibióticos, lo que afecta la eficacia de los tratamientos. Objetivo: Estimar la resistencia antimicrobiana a los antibióticos resultado de las pruebas invitro de susceptibilidad en una institución hospitalaria de tercer nivel de Villavicencio, Colombia. Materiales y Métodos: Se realizó un estudio observacional, descriptivo de tipo retrospectivo, análisis univariado, calculando medias de las variables susceptibilidad, resistencia, antibiótico, microorganismos, y tipo de muestra resultados de las pruebas de susceptibilidad realizadas mediante técnica de Kirby-Bauer de 485 pruebas de susceptibilidad de la institución hospitalaria. Resultados y Discusión: Los microorganismos más frecuentemente aislados, fueron: Escherichia coli, Klebsiella pneumoniae ss. Pneumoniae, Staphylococcus aureus ss. Aureus representando el 49% de total de microorganismos, los mayores reportes de resistencia en la pruebas invitro las presentaron Serratia marcescens, Staphylococcus saprophyticus ss. Saprophytic, Enterobacter cloacae, presentan una resistencia media superior al 50%, y una susceptibilidad inferior 40%, la mayor resistencia media se presentó para los siguientes antibióticos, Cefalotina 72,2%, Cefazolina 59,9%, Nitrofurantoina 54,4%, Ceftriaxona 52,7%. Conclusiones: Antibióticos como la Cefalotina, Nitrofurantoina, Cefazolina y Ceftriaxona  pierden su utilidad terapéutica dada la elevada resistencia demostrada por los microorganismos aislados en las pruebas invitro, es necesario reforzar las medidas de uso adecuado de antibióticos para disminuir la posibilidad de adaptación y resistencia a los mismos,  el fenómeno de resistencia bacteriana ocurre fuera del ámbito hospitalario evidenciando la necesidad de iniciar también control y vigilancia en infecciones ocurridas en la comunidad.Cómo citar este

    5. Diseño de la Formulación de un Material de Empaque Flexible y Comestible a Base de Aislado Proteínico de Suero

      Carlos Regalado González

      2005-01-01

      Full Text Available En la actualidad es muy común resguardar alimentos con empaques de plástico de naturaleza sintética. Actualmente alrededor de un 30% en peso de los desechos municipales corresponden a empaques sintéticos, lo que sugiere la necesidad de crear alternativas ecológicas de empaque, específicamente el diseño y elaboración de películas biodegradables. Dichas películas deben de tener características químicas y mecánicas apropiadas para que retarden la transferencia de masa en los sistemas de alimentos, prolonguen la vida del alimento empacado y mejoren la calidad del producto. Las películas elaboradas con aislado proteínico de suero representan una alternativa viable. Por ello el objetivo de este estudio es determinar la mejor formulación para dichas películas, a partir de los componentes básicos aislado de proteínico de suero (WPI, sorbitol no cristalizable, cera de abeja, cera de candelilla, extracto libre de células (ELC además de la medición de propiedades mecánicas de diferentes combinaciones y concentraciones de los ingredientes básicos. La metodología empleada fue preparar 21 tratamientos de películas biodegradables con aislado proteínico de suero. Posteriormente se realizaron pruebas mecánicas para la determinación del porcentaje de elongación (%E, resistencia a la tensión (RT y módulo de elasticidad (ME para la determinación de la mejor formulación. Se observó que los tratamientos que tienen 10% (w/v de sorbitol (tratamientos 5, 9,14 presentaron mejores propiedades mecánicas como un %E alto y ME alto además de una RT alta, ya que el sorbitol es el que le confiere mayor flexibilidad al empaque, en contraste con aquellas que fueron tratadas con 7% (w/v de sorbitol (tratamientos 2, 8, 12, 21 el cual muestra propiedades mecánicas indeseables, ya que se tornan quebradizas además de que presentan %E bajos así como ME altos

    6. Contribución al conocimiento de la biología y taxonomía de un hongo del género Phytium aislado del "aliso", Alnus acuminata HBK Contribución al conocimiento de la biología y taxonomía de un hongo del género Phytium aislado del "aliso", Alnus acuminata HBK

      Cabrera Orlando; Rodríguez Esperanza; Correa de Restrepo Marina

      1990-01-01

      Se estudio la biología de un hongo aislado de la parte externa de nódulos de raíces de aliso (Alnus acumi"ata HB K), el cual presento un alto poder de germinación a bajas temperaturas (entre QOC y 4QC), condición que le confiere gran agresividad para colonizar el suelo, de tal forma que se constituye en un factor importante en la ecofisiología de las poblaciones de aliso que crecen en los bosques secundarios del sub-paramo. A partir de cultivos puros se hicieron estudios para su iden...

    7. UTILIZACIÓN DE HAPLOTIPOS DE Pyricularia grisea Sacc. AISLADOS EN CUBA PARA LA SELECCIÓN DE CULTIVARES DE ARROZ RESISTENTES A LA PIRICULARIOSIS

      Noraida de J. Pérez León

      2015-01-01

      Full Text Available El principal problema fitopatológico que provoca los bajos rendimientos del cultivo del arroz en Cuba es la Piriculariosi, por tal motivo se evaluaron, en condiciones controladas y con inoculación artificial, tres haplotipos de P. grisea aislados en Cuba de plantas de arroz en dos zonas de producción. Estos fueron posteriormente utilizados en la selección de cultivares de arroz resistentes a la enfermedad. Los resultados permitieron la selección de los haplotipos A 18 y B 6 para la evaluación de cultivares en condiciones semicontroladas y, de esta forma, quedan representados dos linajes del patógeno y, entre ellos, el más agresivo y ampliamente distribuido. Se seleccionaron siete líneas resistentes frente a los haplotipos evaluados, las que constituyen una importante base germoplásmica para la obtención de cultivares resistentes.

    8. Determinación de la sensibilidad a amoxicilina y a clindamicina de staphylococcus spp aislado de cavidad oral de pacientes con alto riesgo de endocarditis infecciosa

      Silvia Barrientos

      2011-01-01

      Full Text Available En la literatura existen pocos estudios acerca de la distribución de Staphylococcus spp en boca y los que hay sobre estos microorganismos como residentes de la cavidad oral son controversiales.El género Staphylococcus spp conforma un importante grupo de patógenos en el ser humano y origina distintas enfermedades, entre ellas, la Endocarditis Infecciosa; de igual forma, la literatura reporta tasas de resistencia a amoxicilina y clindamicina antibióticos usados comúnmente para la profilaxis en odontología. Objetivo: Conocer la sensibilidad de Staphylococcus spp a amoxicilina y la clindamicina aislados de cavidad oral de pacientes con alto riesgo de Endocarditis Infecciosa.

    9. DENSIDAD POBLACIONAL Y ESTRUCTURA DE GRUPO DE Ateles hybridus brunneus (PRIMATES: ATELIDAE) EN UN FRAGMENTO DE BOSQUE AISLADO EN EL SURORIENTE DE ANTIOQUIA, COLOMBIA

      Néstor J. Roncancio; Lina M. García; Amilvia Acosta

      2010-01-01

      Se encontró un grupo de Ateles hybridus brunneus en un fragmento de bosque aislado de 21 ha, con una densidad de 39 individuos/km2 (IC95% = 21.3-72.9). Esta área es la más pequeña donde se ha estimado alta densidad para Ateles. El grupo de A. h. brunneus estuvo compuesto por nueve individuos, grupo más pequeño que el registrado en otras investigaciones. Ateles tiene un sistema social fisión-fusión, donde los grupos se dividen en subgrupos. El tamaño promedio de subgrupo fue de cuatro individu...

    10. Liberación de endotelina-1 por angiotensina ll en miocitos cardíacos aislados Angiotensin II-induced endothelin-1 release in cardiac myocytes

      María C. Villa-Abrille; Cingolani, Horacio E; Carolina D. Garciarena; Ennis, Irene L.; Aiello, Ernesto A.

      2006-01-01

      Muchos de los efectos de la angiotensina II (Ang II) son mediados en realidad por la acción de endotelina (ET) endógena liberada y/o producida en respuesta a la Ang II. En este trabajo evaluamos la interacción Ang II/ET-1, sus consecuencias en la contractilidad cardíaca y el papel de las especies reactivas del oxígeno (EROs). Se usaron cardiomiocitos aislados de gato. La Ang II, 1 nM, produjo un efecto inotrópico positivo (EIP) de 31.8±3.8% que fue cancelado por inhibición de los receptores A...

    11. Selección de hongos aislados de bagazo de caña con actividad celulasa sobre celulosa cristalina para posibles aplicaciones industriales

      Yoandy Ferrer-Marcelo

      2011-01-01

      Full Text Available Las enzimas comerciales necesarias en procesos de producción de bioetanol celulósico y enriquecimiento de forrajes, son excesivamente caras. La mayoría provienen de mutantes B-glucosidasa, con la velocidad y extensión de la hidrólisis reducida, y débil acción sobre la celulosa cristalina. A partir de bagazo, se aislaron varios hongos filamentosos con capacidad de degradación de la celulosa cristalina, para ello se diseñó un medio selectivo de celulosa agar. Del total de aislados, solo 9 microorganismos mostraron un halo definido de degradación de celulosa. Se determinó el coeficiente de degradación de celulosa cristalina a través de la medición del halo formado por las colonias, y se pre-seleccionaron 5 aislados. Posteriormente, se eligieron los tres mejores productores del complejo celulasa (6, 13 y 21 mediante una fermentación sumergida por determinación de la actividad sobre papel de filtro. Se realizó una fermentación en estado sólido sobre bagazo pretratado para aumentar la actividad celulasa. Se estudió la estabilidad relativa de los extractos enzimáticos y se realizó una caracterización parcial de los mismos. Estos presentaron poca estabilidad relativa después de 48 horas, luego de un prensado simple. El mejor rango de pH de hidrólisis estuvo entre 5,5 y 6,2 y presentaron actividad enzimática a una temperatura de 40 ºC.

    12. Actinomycetes Mycetoma

      Sumati Hogade; Metgud, S. C.; Swoorooparani

      2011-01-01

      Mycetoma is a chronic infection, frequently seen in tropical and sub-tropical countries and is considered as an occupational disease. Nocardia species though it can infect immunocompetent individuals, it most commonly affects immunocompromised patients. A 50-year-old male, farmer presented to our hospital with serosanguineous discharging swelling over the dorsum of right foot. We have isolated Nocardia asteroides from the tissue sample. Speciation of this isolate was carried out based on phen...

    13. Components in Antineoplastic Actinomycete Strain(N2010-37)of Bottom Mud in Mangrove

      ZHOU Zhong-liu; JIN Bei; YIN Wen-qing; FU Chun-yan; FENG Hua-fen

      2011-01-01

      Objective To study the antitumor components from an actinomycete strain(N2010-37)of bottom mud in Zhanjiang Mangrove,South China Sea.Methods The components were isolated and purified by chromatographic techniques and recrystallization,and the structures were identified by spectral methods together with physicochemical analyses.The antitumor effects of these components were tested in vitro by MTT method.Results Three compounds were identified including two anthrones and one novel lactone.They are(3S,4R,7R,8R,9S)-3,8-dihydroxy-4,7,9-trimethyl-2,6-cyclononanediolacetone(1),2-hydroxy-l-methoxy-3-methylanthraquinone(2),and 1,6,8-thihydroxy-3-methyl-anthraquinone(3).Conclusion Compound 1 is a new compound,and compounds 1 and 3 show the favorablecytotoxic activities against human chronic granulocytic leukemia cell line K562 strain by MTT method in vitro.

    14. In vitro Susceptibility of Alternaria solani to Several Iranian Soil Actinomycetes

      F. Sharifi

      2006-01-01

      Full Text Available Streptomyces sp. are of the most attractive sources of biologically active compounds. In the recent decades, they have attracted high interests as biocontrol agents. In search of finding such principles, in vitro suppression of Alternaria solani was studied by use of Streptomyces antagonists. A. solani was sensitive to several species mainly Streptomyces plicatus, S. olivaceus and two unidentified actinomycetes. Activity versus time was monitored in S. olivaceus in solid and shaked cultures. Ongoing goals of this research include isolation, characterization and identification of the active metabolites. Future goals include identification of active genes for use in development of recombinant DNAs in transgenic hosts bearing elevated resistance to infections by Alternaria sp.

    15. Nocardia kroppenstedtii sp. nov., an actinomycete isolated from a lung transplant patient with a pulmonary infection.

      Jones, Amanda L

      2014-03-01

      A novel actinomycete, strain N1286(T), isolated from a lung transplant patient with a pulmonary infection, was provisionally assigned to the genus Nocardia. The strain had chemotaxonomic and morphological properties typical of members of the genus Nocardia and formed a distinct phyletic line in the Nocardia 16S rRNA gene tree. Isolate N1286(T) was most closely related to Nocardia farcinica DSM 43665(T) (99.8% gene sequence similarity) but could be distinguished from the latter by the low level of DNA-DNA relatedness. These strains were also distinguishable on the basis of a broad range of phenotypic properties. It is concluded that strain N1286(T) represents a novel species of the genus Nocardia for which the name Nocardia kroppenstedtii sp. nov. is proposed. The type strain is N1286(T) ( = DSM 45810(T) = NCTC 13617(T)).

    16. Structural-functional specificity of the complexes of psychrotolerant soil actinomycetes

      Zenova, G. M.; Dubrova, M. S.; Zvyagintsev, D. G.

      2010-04-01

      The active growth and development of psychrotolerant actinomycetes take place in peat and podzolic soils of the tundra and taiga at temperatures below 10°C. The population density of psychrotolerant mycelial prokaryotes in these soils reaches thousands and tens of thousands of CFU/g of soil, and the length of their mycelium is up to 380 m/g of soil. The application of fluorescent in situ hybridization (the FISH method) demonstrated that the metabolically active psychrotolerant representatives of the phylogenetic group of Actinobacteria comprise up to 30% of the total number of bacteria in prokaryotic microbial communities of oligotrophic peat bog and podzolic soils. The portion of metabolically active mycelial actinobacteria exceeds the portion of unicellular actinobacteria. Psychrotolerant streptomycetes isolated from peat bog soils possess pectinolytic, amylolytic, and antagonistic activities at low temperatures (5°C).

    17. ISOLEMENT DES SOUCHES D’ACTINOMYCETES PRODUCTRICES DE NOUVELLES MOLECULES ANTIFONGIQUES

      N. CHERAITI

      2012-01-01

      Full Text Available Twenty two strains of actinomycetes were isolated from four samples of Oubeira lake fresh waters of El Kala region (East of Algeria. The antifungal activity was tested against one filamentous fungus: Aspergillus niger (strain supplied by the mycology laboratory of IBN SINA hospital of Annaba. We have utilized two methods in order to study the antifungal activity: culture in liquid medium and double layer method by testing four different media: ISPI, ISPII, GYEA and Bennett.Among the twenty two isolates, only two strains showed an activity against the target strain. It concerns strains Act sp5 and Act sp10. The activity of strain Act sp5 was very important with an inhibition zone diameter equals at 60 mm on GYEA medium.

    18. [Secondary metabolites from a deep-sea-derived actinomycete Micrococcus sp. R21].

      Peng, Kun; Su, Rui-qiang; Zhang, Gai-yun; Cheng, Xuan-xuan; Yang, Quan; Liu, Yong-hong; Yang, Xian-wen

      2015-06-01

      To investigate cytotoxic secondary metabolites of Micrococcus sp. R21, an actinomycete isolated from a deep-sea sediment (-6 310 m; 142 degrees 19. 9' E, 10 degrees 54. 6' N) of the Western Pacific Ocean, column chromatography was introduced over silica gel, ODS, and Sephadex LH-20. As a result, eight compounds were obtained. By mainly detailed analysis of the NMR data, their structures were elucidated as cyclo(4-hydroxy-L-Pro-L-leu) (1), cyclo(L-Pro-L-Gly) (2), cyclo( L-Pro-L-Ala) (3), cyclo( D-Pro-L-Leu) (4), N-β-acetyltryptamine (5), 2-hydroxybenzoic acid (6), and phenylacetic acid (7). Compound 1 exhibited weak cytotoxic activity against RAW264. 7 cells with IC50 value of 9.1 μmol x L(-1). PMID:26591527

    19. Nocardia pigrifrangens sp. nov., a novel actinomycete isolated from a contaminated agar plate.

      Wang, Liming; Zhang, Yamei; Huang, Ying; Maldonado, Luis A; Liu, Zhiheng; Goodfellow, Michael

      2004-09-01

      A polyphasic study was undertaken to establish the taxonomic position of an actinomycete strain isolated from a contaminated agar plate. The strain, designated 7031T, had morphological and chemotaxonomic properties typical of the genus Nocardia. An almost-complete 16S rRNA gene sequence determined for the strain was aligned with available sequences for nocardiae, and phylogenetic trees were inferred using three tree-generating algorithms. Strain 7031T clustered with the type strains of Nocardia carnea and Nocardia flavorosea, showing low 16S rRNA gene sequence similarities to these species (97.2 and 97.5 %, respectively). The strain was also distinguished from the closest species by a range of phenotypic properties. It is proposed that the strain be recognized as a novel species of Nocardia, Nocardia pigrifrangens sp. nov., the type strain of which is 7031T (= AS 4.1808T = JCM 11884T). PMID:15388728

    20. Marine actinomycetes: a new source of compounds against the human malaria parasite.

      Jacques Prudhomme

      Full Text Available BACKGROUND: Malaria continues to be a devastating parasitic disease that causes the death of 2 million individuals annually. The increase in multi-drug resistance together with the absence of an efficient vaccine hastens the need for speedy and comprehensive antimalarial drug discovery and development. Throughout history, traditional herbal remedies or natural products have been a reliable source of antimalarial agents, e.g. quinine and artemisinin. Today, one emerging source of small molecule drug leads is the world's oceans. Included among the source of marine natural products are marine microorganisms such as the recently described actinomycete. Members of the genus Salinispora have yielded a wealth of new secondary metabolites including salinosporamide A, a molecule currently advancing through clinical trials as an anticancer agent. Because of the biological activity of metabolites being isolated from marine microorganisms, our group became interested in exploring the potential efficacy of these compounds against the malaria parasite. METHODS: We screened 80 bacterial crude extracts for their activity against malaria growth. We established that the pure compound, salinosporamide A, produced by the marine actinomycete, Salinispora tropica, shows strong inhibitory activity against the erythrocytic stages of the parasite cycle. Biochemical experiments support the likely inhibition of the parasite 20S proteasome. Crystal structure modeling of salinosporamide A and the parasite catalytic 20S subunit further confirm this hypothesis. Ultimately we showed that salinosporamide A protected mice against deadly malaria infection when administered at an extremely low dosage. CONCLUSION: These findings underline the potential of secondary metabolites, derived from marine microorganisms, to inhibit Plasmodium growth. More specifically, we highlight the effect of proteasome inhibitors such as salinosporamide A on in vitro and in vivo parasite development

    1. Antimicrobial Activities of Some Actinomycetes Isolated from Different Rhizospheric Soils in Tunisia.

      Trabelsi, Ines; Oves, Daniel; Manteca, Angel; Genilloud, Olga; Altalhi, Abdullah; Nour, Mohamed

      2016-08-01

      Fifty four isolates of actinomycetes were collected from four different rhizospheric soils: 18 strains from palm tree bark and soil, 12 strains from an olive field soil, 9 strains from a coastal forest, and 15 strains from an agriculture soil situated in the Algerian-Tunisian border (Oum Tboul). Based on morphological and cultural characters, the isolates were classified as Streptomyces (42 strains), Micromonospora (4 strains), Pseudonocardia (1 strain), Actinomadura (1 strain), Nocardia (1 strain), and non-Streptomyces (5 strains). More than half of the isolates inhibited at least one tested pathogenic microorganisms in liquid culture. In addition, antimicrobial activities of some strains were tested on solid culture. Several bioactive compounds were identified by liquid chromatography joined with low-resolution mass spectroscopy (LC/MS) and analysed by MEDINA's database and by the dictionary of natural products Chapman & Hall. An interesting chlorinated compound with the molecular formula C20H37ClN2O4, produced by three different strains (SF1, SF2, and SF5), was subject of an attempted purification. However, it was demonstrated using confocal microscopy and LC/MS high resolution that this compound is produced only on solid culture. These three potential antimicrobial isolates showed high similarity with Streptomyces thinghirensis and Streptomyces lienomycini, in terms of morphological characteristics and 16S rRNA gene sequences (bootstrap 97 %). All these findings prove the high antimicrobial diversity of the studied soils. The potential of the selected and other relatively unexplored extreme environments constitute a source of interesting actinomycete strains producing several biologically active secondary metabolites. PMID:27139253

    2. Himalomycin A and cycloheximide-producing marine actinomycete from Lagos Lagoon soil sediment

      Davies Olabisi Flora; Adeleye IsaacAdeyemi; Wang Peng George

      2015-01-01

      Objective: To isolate and screen Actinomycetes from Lagos Lagoon soil sediments for antibiotic production. Methods: Soil samples were collected from four different locations of Lagos Lagoon and were dried for 2 weeks. Actinomycetes were isolated by serial dilution using spread plate method on starch casein and Kuster’s agar supplemented with 80 μg/mL cycloheximide to prevent fungal growth. The plates were incubated at 28 °C for 1-2 weeks. Isolates were selected based on their cultural characteristics as well as their Gram’s reaction and subcultured on same media for isolation and incubated at 28 °C for 3 days. Pure cultures were maintained on nutrient agar slants at 4 °C. Thereafter, they were inoculated into starch casein and Kuster’s broth media and incubated at 28 °C for 8 days. The resulting crude extracts were screened for antimicrobial activity against the following microorganisms: methicillin resistant Staphylococcus aureus, Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 29522, Pseudomonas aeruginosa ATCC 27853, Candida albicans and Enterococcus faecalis ATCC 29212. Coagulase-negative staphylococci isolated from HIV patients were also used (Staphylococcus warneri, Staphylococcus xylosus and Staphylococcus epidermidis). Extraction of secondary metabolites was carried out and analysed using gas chromatography-mass spectrometer. Results: All the isolates displayed varying antimicrobial activity against at least one of the test organisms. Himalomycin A was identified in the extract from isolate ULS7. The gas chromatography-mass spectrometer data analysis showed the antibiotic profile of these isolates. Conclusions: The isolate ULS7 was found to display the highest antimicrobial activity against the test organisms.

    3. SCREENING, ISOLATION AND PURIFICATION OF ANTIBIOTIC(S FROM MARINE ACTINOMYCETES

      Attimarad S L

      2012-06-01

      Full Text Available As marine environmental conditions are extremely different from terrestrial ones, it is surmised that marine actinomycetes might produce novel bioactive compounds. Hence marine sediments, collected from the coastal areas of Gokharna and Muradeshwara of Karnataka state, were screened. Seventeen isolates were obtained on starch-casein agar media by soil dilution technique. However, only six isolates namely SUN-A2, SUN-A3, SUN-A4, SUN-A5, SUN-A7 and SUN-A15 showed significant antibacterial activity against both gram-positive and gram-negative bacteria. Further studies were carried out with the most active SUN-A2. Optimization of media, temperature and pH by shake flask fermentation indicated starch-casein, 28o C and pH 7 to be suitable for SUN-A2. The production of antibiotics began after 24 h reached maximum at 72 h and maintained at the same level up to 120 h. Ethyl acetate was used to extract antibacterial compounds from the culture filtrate. TLC was done on silica gel using ethyl acetate: methanol (6:4 and direct bioautography showed the presence of two active substance, one with Rf 0.8 more active than the other with Rf 0.4. Further purification is done by column chromatography using a mixture of dicholoromethane and ethyl acetate. The findings from this investigation reveal that the strain SUN-A2 in order exhibited superior antimicrobial activity to other sediment isolates of actinomycetes.

    4. Antibiotic Producing Potentials of Three Freshwater Actinomycetes Isolated from the Eastern Cape Province of South Africa

      Timothy Sibanda

      2010-07-01

      Full Text Available Crude extracts of three actinomycetes species belonging to Saccharopolyspora (TR 046 and TR 039 and Actinosynnema (TR 024 genera were screened for antibacterial activities against a panel of several bacterial strains. The extracts showed antibacterial activities against both gram-negative and gram-positive test bacteria with inhibition zones ranging from 8 to 28 mm (TR 046; 8 to15 mm (TR 039; and 10 to 13 mm (TR 024. The minimum inhibitory concentrations ranged from 0.078 to 10 mg/mL (TR 046; 5 to >10 mg/mL (TR 039; and 1.25 to 5 mg/mL (TR 024. Time-kill studies revealed that crude extract of TR 046 showed strong bactericidal activity against Bacillus pumilus (ATCC14884, reducing the bacterial load by 104 cfu/mL and 102 cfu/mL at 4× MIC and 2× MIC, respectively, after 6 h of exposure. Similarly, against Proteus vulgaris (CSIR 0030, crude extract of TR 046 achieved a 0.9log10 and 0.13log10 cfu/mL reduction at 5 mg/mL (4× MIC and 1.25 mg/mL (2× MIC after 12 h of exposure. The extract was however weakly bactericidal against two environmental bacterial strains (Klebsiella pneumoniae and Staphylococcus epidermidis; and against Pseudomonas aeruginosa (ATCC 19582: the extract showed bacteriostatic activities at all concentrations tested. These freshwater actinomycetes appear to have immense potential as a source of new antibacterial compound(s.

    5. Influence of mode of storage and drying of fodder on thermophilic actinomycete aerocontamination in dairy farms of the Doubs region of France.

      Dalphin, J.C.; Pernet, D; Reboux, G; Martinez, J.; Dubiez, A.; Barale, T; Depierre, A

      1991-01-01

      Airborne contamination by thermophilic actinomycetes, micromycetes and Gram negative bacteria was determined on 34 dairy farms and related to fodder drying and storage methods. Eighteen farms had a barn drying system, eight with additional heating; the remaining 16 had traditional fodder storage methods. Three air samples were obtained for each farm with a six stage Andersen sampler. The thermophilic actinomycetes were identified as Streptomyces and the dominant micromycetes as Aspergillus sp...

    6. Desde el compromiso de las transferencias

      Bonet Bertomeu, Josep María

      2005-01-01

      En Enero de 2002, los Servicios Sanitarios gestionados por el INSALUD fueron transferidos a las diez Comunidades Autónomas que todavía no los habían recibido. Se completó así el mapa territorial de Servicios de Salud Autonómicos, si exceptuamos Ceuta y Melilla que siguen gestionados por la Administración Central. Habiendo transcurrido más de tres años desde aquella fecha se puede ver con cierta perspectiva aquel proceso, algunos de sus componentes y aportar así algo al momento que vive n...

    7. CULTURA POLITICA: ACERCAMIENTO CONCEPTUAL DESDE AMERICA LATINA

      Cecilia Millán La Rivera

      2008-01-01

      Este artículo analiza el concepto de cultura política planteado por los autores Almond y Verba, entregando una breve contextualización del término, para luego centrarse en ciertas críticas surgidas desde Europa y América Latina. A partir de lo anterior, se invita a la reflexión sobre la problemática del concepto de Cultura Política en América Latina, cuestionándose su pertinencia literal, debido al origen ajeno a nuestra realidad. Luego de este análisis, se describen algunos elementos que deb...

    8. Escritos desde la “frontera”

      Serrano, Santiago

      2015-01-01

      Reflexiones sobre la práctica dramatúrgica. Concepción del espacio escénico como espacio de frontera. Sitio donde convergen distintas disciplinas artísticas y el público. Espacio lleno de condicionamientos, pero que permite desplegar desde él la creatividad. Breve reseña de mi trayectoria como dramaturgo y las fronteras que se presentaron a mi paso, no sólo geográficas sino también de lenguaje. La plataforma virtual como soporte para nuevas experiencias creativas. Comentario sobre el proceso ...

    9. Clear felling and burning effects on soil nitrogen transforming bacteria and actinomycetes population in Chittagong University campus, Bangladesh

      S.M.Sirajul Haque; Rahima Ferdoshi; Sohag Miah; M.Nural Anwar

      2012-01-01

      The effect of forests clear felling and associated burning on the population of soil nitrogen transforming bacteria and actinomycetes are reported at three pair sites of Chittagong University campus,Bangladesh in monsoon tropical climate.Clear felled area or burnt site and 15-21 year mixed plantation of native and exotic species,situated side by side on low hill having Typic Dystrochrepts soil was represented at each pair site.At all the three pair sites,clear felled area or burnt site showed very significantly (p≤0.001) lower population of actinomycetes,Rhizobium,Nitrosomonas,Nitrobacter and ammonifying as well as denitrifying bacteria compared to their adjacent mixed plantation.From environmental consideration,this finding has implication in managing natural ecosystem.

    10. Structural changes of actinomycete communities along primary succession process on non-reclaimed post-mining sites

      Chroňáková, Alica; Krištůfek, Václav; Elhottová, Dana; Tichý, Martin

      Lyon : Microbial- ecology Organisation , 2005. s. 94. [Bageco /8./. 26.06.2005-29.06.2005, Lyon] R&D Projects: GA ČR(CZ) GA526/03/1259 Grant ostatní: GAJU(CZ) GAJU/54/2004 Institutional research plan: CEZ:AV0Z60660521 Keywords : actinomycete communities * primary succession process * non-reclaimed post-mining sites Subject RIV: EH - Ecology , Behaviour

    11. Structures and comparative characterization of biosynthetic gene clusters for cyanosporasides, enediyne-derived natural products from marine actinomycetes

      Lane, Amy L.; Nam, Sang Jip; Fukuda, Takashi; Yamanaka, Kazuya; Kauffman, Christopher A.; Jensen, Paul R; Fenical, William; Moore, Bradley S.

      2013-01-01

      Cyanosporasides are marine bacterial natural products containing a chlorinated cyclopenta[a]indene core of suspected enediyne polyketide biosynthetic origin. Herein, we report the isolation and characterization of novel cyanosporasides C–F (3–6) from the marine actinomycetes “Salinispora pacifica” CNS-143 and Streptomyces sp. CNT-179, highlighted by the unprecedented C-2' N-acetylcysteamine functionalized hexose group of 6. Cloning, sequencing, and mutagenesis of homologous ~50 kb cyanosporas...

    12. Structural and Functional Characterizations of SsgB, a Conserved Activator of Developmental Cell Division in Morphologically Complex Actinomycetes

      Xu, Qingping; Traag, Bjørn A.; Willemse, Joost; McMullan, Daniel; Miller, Mitchell D.; Elsliger, Marc-André; Abdubek, Polat; Astakhova, Tamara; Axelrod, Herbert L.; Bakolitsa, Constantina; Carlton, Dennis; Chen, Connie; Chiu, Hsiu-Ju; Chruszcz, Maksymilian; Clayton, Thomas; Das, Debanu; Deller, Marc C.; Duan, Lian; Ellrott, Kyle; Ernst, Dustin; Farr, Carol L.; Feuerhelm, Julie; Grant, Joanna C.; Grzechnik, Anna; Grzechnik, Slawomir K.; Han, Gye Won; Jaroszewski, Lukasz; Jin, Kevin K.; Klock, Heath E.; Knuth, Mark W.; Kozbial, Piotr; Krishna, S. Sri; Kumar, Abhinav; Marciano, David; Minor, Wladek; Mommaas, A. Mieke; Morse, Andrew T.; Nigoghossian, Edward; Nopakun, Amanda; Okach, Linda; Oommachen, Silvya; Paulsen, Jessica; Puckett, Christina; Reyes, Ron; Rife, Christopher L.; Sefcovic, Natasha; Tien, Henry J.; Trame, Christine B.; van den Bedem, Henry; Wang, Shuren; Weekes, Dana; Hodgson, Keith O.; Wooley, John; Deacon, Ashley M.; Godzik, Adam; Lesley, Scott A.; Wilson, Ian A.; van Wezel, Gilles P.; (Leiden-MC); (SLAC); (Scripps); (UV); (UCSD); (Burnham)

      2010-01-20

      SsgA-like proteins (SALPs) are a family of homologous cell division-related proteins that occur exclusively in morphologically complex actinomycetes. We show that SsgB, a subfamily of SALPs, is the archetypal SALP that is functionally conserved in all sporulating actinomycetes. Sporulation-specific cell division of Streptomyces coelicolor ssgB mutants is restored by introduction of distant ssgB orthologues from other actinomycetes. Interestingly, the number of septa (and spores) of the complemented null mutants is dictated by the specific ssgB orthologue that is expressed. The crystal structure of the SsgB from Thermobifida fusca was determined at 2.6 {angstrom} resolution and represents the first structure for this family. The structure revealed similarities to a class of eukaryotic 'whirly' single-stranded DNA/RNA-binding proteins. However, the electro-negative surface of the SALPs suggests that neither SsgB nor any of the other SALPs are likely to interact with nucleotide substrates. Instead, we show that a conserved hydrophobic surface is likely to be important for SALP function and suggest that proteins are the likely binding partners.

    13. Degradative crystal–chemical transformations of clay minerals under the influence of cyanobacterium-actinomycetal symbiotic associations

      Ekaterina Ivanova

      2014-04-01

      Full Text Available Cyanobacteria and actinomycetes are essential components of soil microbial community and play an active role in ash elements leaching from minerals of the parent rock. Content and composition of clay minerals in soil determine the sorption properties of the soil horizons, water-holding capacity of the soil, stickiness, plasticity, etc. The transformative effect of cyanobacterial–actinomycetes associations on the structure of clay minerals – kaolinite, vermiculite, montmorillonite, biotite and muscovite – was observed, with the greatest structural lattice transformation revealed under the influence of association in comparison with monocultures of cyanobacterium and actinomycete. The range of the transformative effect depended both on the type of biota (component composition of association and on the crystal–chemical parameters of the mineral itself (trioctahedral mica – biotite, was more prone to microbial degradation than the dioctahedral – muscovite. The formation of the swelling phase – the product of biotite transformation into the mica–vermicullite mixed-layered formation was revealed as a result of association cultivation. Crystal chemical transformation of vermiculite was accompanied by the removal of potassium (К, magnesium (Mg and aluminum (Al from the crystal lattice. The study of such prokaryotic communities existed even in the early stages of the Earth's history helps to understand the causes and nature of the transformations undergone by the atmosphere, hydrosphere and lithosphere of the planet.contribution of treatments on structure induces and model parameters are discussed in the paper.

    14. Evaluation of antimicrobial activity of the endophytic actinomycete R18(6) against multiresistant Gram-negative bacteria.

      Carvalho, Tiele; Van Der Sand, Sueli

      2016-03-01

      Endophytic actinomycetes are promising sources of antimicrobial substances. This study evaluates the activity of metabolites produced by the endophytic actinomycete R18(6) against Gram-negative bacteria multiresistant to antimicrobials. R18(6) isolate was grown in submerged cultures under different conditions: carbon source, temperature, pH and incubation time to optimize antimicrobials production. The actinomycete grown in base medium supplemented with 1% glucose, pH 6.5 and incubation at 30 ºC for 96 h with shaking at 100 rpm, exhibited the highest activity against the used Gram-negative bacteria. Minimum inhibitory concentration (MIC) of the crude extract produced by the microorganism varied between 1/32 and 1/256. It had bactericide or bacteriostatic activity, depending on the Gram-negative organism. The active extract was stable at high temperatures, and unstable in medium containing proteolytic enzymes. Micromorphology of R18(6) was investigated by optical and scan microscopy, revealing that it was morphologically similar to the genusStreptomyces. PMID:26871499

    15. Sensitivity of Venturia inaequalis Chilean Isolates to Difenoconazole, Fenarimol, Mancozeb, and Pyrimethanil Sensibilidad de Aislados Chilenos de Venturia inaequalis a Difenoconazole, Fenarimol, Mancozeb y Pyrimethanil

      Jose Luis Henríquez S

      2011-03-01

      Full Text Available Apple scab (Venturia inaequalis is the most important disease in Chilean apple (Malus domestica Borkh. orchards where fungicide management is the main tool to control disease. Sensitivity tests to difenoconazole, fenarimol, mancozeb, and pyrimethanil fungicides were conducted on V. inaequalis wild isolates to construct local baseline sensitivity distributions, and compare them with a collection of isolates from seven different commercial orchards with no evidence of practical resistance. In vitro assays were conducted and sensitivity was measured as inhibition of mycelial growth (difenoconazole, fenarimol, pyrimethanil or conidial germination (mancozeb. Departure from the baseline distribution to less sensitivity was found for difenoconazole, fenarimol, and mancozeb with resistance factors of 4.7, 5.8, and 2.1, respectively. The highest difference between baseline and orchard populations was observed for fenarimol which showed the highest shift to less sensitivity. Discriminatory doses of 0.04, 1.0, 0.6, and 0.2 µg mL-1 are proposed for in vitro monitoring of sensitivity to difenoconazole, fenarimol, mancozeb, and pyrimethanil, respectively.La sarna del manzano (Venturia inaequalis es la principal enfermedad del cultivo en Chile y es controlada principalmente con el uso de fungicidas. Se realizaron pruebas de sensibilidad a los fungicidas difenoconazole, fenarimol, mancozeb y pyrimethanil en aislados monoconidiales de cepas silvestres de V. inaequalis con el fin de elaborar curvas basales de sensibilidad y compararlas con la sensibilidad de una colección de aislados monoconidiales provenientes de siete huertos comerciales sin evidencias de resistencia práctica. Se realizaron pruebas in vitro donde la sensibilidad se midió como la inhibición del crecimiento miceliar (difenoconazole, fenarimol y pyrimethanil o la inhibición de la germinación de conidias (mancozeb. La población de huertos comerciales estudiada presentó una menor

    16. Inhibition of Aspergillus parasiticus and cancer cells by marine actinomycete strains

      Li, Ping; Yan, Peisheng

      2014-12-01

      Ten actinomycete strains isolated from the Yellow Sea off China's coasts were identified as belonging to two genera by 16S rDNA phylogenetic analysis: Streptomyces and Nocardiopsis. Six Streptomyces strains (MA10, 2SHXF01-3, MA35, MA05-2, MA05-2-1 and MA08-1) and one Nocardiopsis strain (MA03) were predicted to have the potential to produce aromatic polyketides based on the analysis of the KSα (ketoacyl-synthase) gene in the type II PKS (polyketides synthase) gene cluster. Four strains (MA03, MA01, MA10 and MA05-2) exhibited significant inhibitory effects on mycelia growth (inhibition rate >50%) and subsequent aflatoxin production (inhibition rate >75%) of the mutant aflatoxigenic Aspergillus parasiticus NFRI-95. The ethyl acetate extracts of the broth of these four strains displayed significant inhibitory effects on mycelia growth, and the IC50 values were calculated (MA03: 0.275 mg mL-1, MA01: 0.106 mg mL-1, MA10: 1.345 mg mL-1 and MA05-2: 1.362 mg mL-1). Five strains (2SHXF01-3, MA03, MA05-2, MA01 and MA08-1) were selected based on their high cytotoxic activities. The ethyl acetate extract of the Nocardiopsis strain MA03 was particularly noted for its high antitumor activity against human carcinomas of the cervix (HeLa), lung (A549), kidney (Caki-1) and liver (HepG2) (IC50: 2.890, 1.981, 3.032 and 2.603 μg mL-1, respectively). The extract also remarkably inhibited colony formation of HeLa cells at an extremely low concentration (0.5 μg mL-1). This study highlights that marine-derived actinomycetes are a huge resource of compounds for the biological control of aflatoxin contamination and the development of novel drugs for human carcinomas.

    17. Identification of actinomycetes from plant rhizospheric soils with inhibitory activity against Colletotrichum spp., the causative agent of anthracnose disease

      Mungsuntisuk Isada

      2011-04-01

      Full Text Available Abstract Background Colletotrichum is one of the most widespread and important genus of plant pathogenic fungi worldwide. Various species of Colletotrichum are the causative agents of anthracnose disease in plants, which is a severe problem to agricultural crops particularly in Thailand. These phytopathogens are usually controlled using chemicals; however, the use of these agents can lead to environmental pollution. Potential non-chemical control strategies for anthracnose disease include the use of bacteria capable of producing anti-fungal compounds such as actinomycetes spp., that comprise a large group of filamentous, Gram positive bacteria from soil. The aim of this study was to isolate actinomycetes capable of inhibiting the growth of Colletotrichum spp, and to analyze the diversity of actinomycetes from plant rhizospheric soil. Results A total of 304 actinomycetes were isolated and tested for their inhibitory activity against Colletotrichum gloeosporioides strains DoA d0762 and DoA c1060 and Colletotrichum capsici strain DoA c1511 which cause anthracnose disease as well as the non-pathogenic Saccharomyces cerevisiae strain IFO 10217. Most isolates (222 out of 304, 73.0% were active against at least one indicator fungus or yeast. Fifty four (17.8% were active against three anthracnose fungi and 17 (5.6% could inhibit the growth of all three fungi and S. cerevisiae used in the test. Detailed analysis on 30 selected isolates from an orchard at Chanthaburi using the comparison of 16S rRNA gene sequences revealed that most of the isolates (87% belong to the genus Streptomyces sp., while one each belongs to Saccharopolyspora (strain SB-2 and Nocardiopsis (strain CM-2 and two to Nocardia (strains BP-3 and LK-1. Strains LC-1, LC-4, JF-1, SC-1 and MG-1 exerted high inhibitory activity against all three anthracnose fungi and yeast. In addition, the organic solvent extracts prepared from these five strains inhibited conidial growth of the three

    18. Desde el compromiso de las transferencias

      Bonet Bertomeu, Josep María

      2005-02-01

      Full Text Available En Enero de 2002, los Servicios Sanitarios gestionados por el INSALUD fueron transferidos a las diez Comunidades Autónomas que todavía no los habían recibido. Se completó así el mapa territorial de Servicios de Salud Autonómicos, si exceptuamos Ceuta y Melilla que siguen gestionados por la Administración Central. Habiendo transcurrido más de tres años desde aquella fecha se puede ver con cierta perspectiva aquel proceso, algunos de sus componentes y aportar así algo al momento que vive nuestro sistema, siempre con ánimo de enriquecer el debate.…

    19. CULTURA POLITICA: ACERCAMIENTO CONCEPTUAL DESDE AMERICA LATINA

      Cecilia Millán La Rivera

      2008-01-01

      Full Text Available Este artículo analiza el concepto de cultura política planteado por los autores Almond y Verba, entregando una breve contextualización del término, para luego centrarse en ciertas críticas surgidas desde Europa y América Latina. A partir de lo anterior, se invita a la reflexión sobre la problemática del concepto de Cultura Política en América Latina, cuestionándose su pertinencia literal, debido al origen ajeno a nuestra realidad. Luego de este análisis, se describen algunos elementos que debiesen tenerse en cuenta para adecuar el concepto a la realidad latinoamericana, a fin de hacer más pertinente su uso.

    20. Linux desde código fuente

      Constantin Bungarzan, Adrian

      2014-01-01

      Compilación e instalación de un sistema operativo Linux desde código fuente. El resultado final es una versión de Linux mínima con interfaz gráfica y con algunas utilidades y librerías para realizar tareas de auditoría en redes y en el sistema. Compilació i instal·lació d'un sistema operatiu Linux des de codi font. El resultat final és una versió de Linux mínima amb interfície gràfica i amb algunes utilitats i llibreries per realitzar tasques d'auditoria en xarxes i en el sistema....

    1. Actinorugispora endophytica gen. nov., sp. nov., an actinomycete isolated from Daucus carota.

      Liu, Min-Jiao; Zhu, Wen-Yong; Li, Jie; Zhao, Guo-Zhen; Xiong, Zhi; Park, Dong-Jin; Hozzein, Wael N; Kim, Chang-Jin; Li, Wen-Jun

      2015-08-01

      An actinomycete strain, designated YIM 690008T, was isolated from Daucus carota collected from South Korea and its taxonomic position was investigated by using a polyphasic approach. The strain grew well on most media tested and no diffusible pigment was produced. The aerial mycelium formed wrinkled single spores and short spore chains, some of which were branched. The whole-cell hydrolysates contained meso-diaminopimelic acid, glucose, mannose, ribose, galactose and rhamnose. The predominant menaquinones were MK-10(H4), MK-10(H6), MK-10(H8) and MK-10(H2). The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannosides, some unknown phospholipids, glycolipids and polar lipids. The major fatty acids were i-C16 : 0, ai-C17 : 0 and C18 : 1ω9c. The DNA G+C content of the genomic DNA was 63.1 mol%. Phylogenetic analysis indicated that the isolate belongs to the family Nocardiopsaceae. However, based on phenotypic, chemotaxonomic and genotypic data, it was concluded that strain YIM 690008T represents a novel genus and novel species of the family Nocardiopsaceae, for which the name Actinorugispora endophytica gen. nov., sp. nov. (type strain YIM 690008T = DSM 46770T = JCM 30099T = KCTC 29480T) is proposed. PMID:25948617

    2. Actinomadura gamaensis sp. nov., a novel actinomycete isolated from soil in Gama, Chad.

      Abagana, Adam Yacoub; Sun, Pengyu; Liu, Chongxi; Cao, Tingting; Zheng, Weiwei; Zhao, Shanshan; Xiang, Wensheng; Wang, Xiangjing

      2016-06-01

      A novel single spore-producing actinomycete, designated strain NEAU-Gz5(T), was isolated from a soil sample from Gama, Chad. A polyphasic taxonomic study was carried out to establish the status of this strain. The diamino acid present in the cell wall is meso-diaminopimelic acid. Glucose, mannose and madurose occur in whole cell hydrolysates. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and an unidentified glycolipid. The predominant menaquinones were identified as MK-9(H8) and MK-9(H6). The predominant cellular fatty acids were found to be C16:0, iso-C15:0, iso-C16:0 and C18:0 10-methyl. Phylogenetic analysis based on the 16S rRNA gene showed that strain NEAU-Gz5(T) belongs to the genus Actinomadura and is closely related to Actinomadura oligospora JCM 10648(T) (ATCC 43269(T); 98.3 % similarity). However, the low level of DNA-DNA relatedness and some different phenotypic characteristics allowed the strain to be distinguished from its close relatives. Therefore, it is concluded that strain NEAU-Gz5(T) represents a novel species of the genus of Actinomadura, for which the name Actinomadura gamaensis sp. nov. is proposed. The type strain is NEAU-Gz5(T) (= CGMCC 4.7301(T) = DSM 100815(T)). PMID:27010208

    3. Hydrophobic nature and effects of culture conditions on biofilm formation by the cellulolytic actinomycete Thermobifida fusca

      Almaris N. Alonso

      2015-09-01

      Full Text Available Thermobifida fusca produces a firmly attached biofilm on nutritive and non-nutritive surfaces, such as cellulose, glass, plastic, metal and Teflon®. The ability to bind to surfaces has been suggested as a competitive advantage for microbes in soil environments. Results of previous investigations indicated that a Gram-positive cellulolytic soil bacteria, Cellulomonas uda, a facultative aerobe, specifically adhered to nutritive surfaces forming biofilms, but cells did not colonize non-nutritive surfaces. Cell surface hydrophobicity has been implicated in the interactions between bacteria and the adhesion to surfaces. It was recently described that the cellulolytic actinomycete T. fusca cells hydrophobicity was measured and compared to the cellulolytic soil bacteria C. uda. Also, T. fusca biofilm formation on non-nutritive surface, such as polyvinyl chloride, was examined by testing various culture ingredients to determine a possible trigger mechanism for biofilm formation. Experimental results showed that partitioning of bacterial cells to various hydrocarbons was higher in T. fusca cells than in C. uda. The results of this study suggest that the attachment to multiple surfaces by T. fusca could depend on nutrient availability, pH, salt concentrations, and the higher hydrophobic nature of bacterial cells. Possibly, these characteristics may confer T. fusca a selective advantage to compete and survive among the many environments it thrives.

    4. Diversity and bioactivity of actinomycetes from marine sediments of the Yellow Sea

      Zhang, Shumin; Ye, Liang; Tang, Xuexi

      2012-03-01

      Among the 116 actinomycetes collected from marine sediments of the Yellow Sea, 56 grew slowly and appeared after 2-3 weeks of incubation. Among the 56 strains, only 3 required seawater (SW) for growth, and 21 grew well in the medium prepared with SW rather than distilled water (DW), while the remaining 32 grew well either with SW or with DW. Six representatives with different morphological characteristics, including 1 SW-requiring strain and 5 well-growing with SW strains, were selected for phylogenetic analysis based on 16S rRNA gene. Two strains belong to Micrococcaceae and Nocardiopsaceae respectively. The other 4 strains belong to the family of Streptomycetaceae. In the analyzed 6 strains, one was related to Nocardiopsis spp. and the other three were related to Streptomyces spp., representing new taxa. Bioactivity testing of fermentation products from 3 SW-requiring strains and 21 well-growing with SW strains revealed that 17 strains possessed remarkable activities against gram-positive pathogen or/and tumor cells, suggesting that they were prolific resources for natural drug discovery.

    5. Genome Sequence and Analysis of the Soil Cellulolytic ActinomyceteThermobifida fusca

      Lykidis, Athanasios; Mavromatis, Konstantinos; Ivanova, Natalia; Anderson, Iain; Land, Miriam; DiBartolo, Genevieve; Martinez, Michele; Lapidus, Alla; Lucas, Susan; Copeland, Alex; Richardson, Paul; Wilson,David B.; Kyrpides, Nikos

      2007-02-01

      Thermobifida fusca is a moderately thermophilic soilbacterium that belongs to Actinobacteria. 3 It is a major degrader ofplant cell walls and has been used as a model organism for the study of 4secreted, thermostable cellulases. The complete genome sequence showedthat T. fusca has a 5 single circular chromosome of 3642249 bp predictedto encode 3117 proteins and 65 RNA6 species with a coding densityof 85percent. Genome analysis revealed the existence of 29 putative 7glycoside hydrolases in addition to the previously identified cellulasesand xylanases. The 8 glycosyl hydrolases include enzymes predicted toexhibit mainly dextran/starch and xylan 9 degrading functions. T. fuscapossesses two protein secretion systems: the sec general secretion 10system and the twin-arginine translocation system. Several of thesecreted cellulases have 11 sequence signatures indicating theirsecretion may be mediated by the twin-arginine12 translocation system. T.fusca has extensive transport systems for import of carbohydrates 13coupled to transcriptional regulators controlling the expression of thetransporters and14 glycosylhydrolases. In addition to providing anoverview of the physiology of a soil 15 actinomycete, this study presentsinsights on the transcriptional regulation and secretion of16 cellulaseswhich may facilitate the industrial exploitation of thesesystems.

    6. Antibiotic pigment from desert soil actinomycetes; biological activity, purification and chemical screening

      Selvameenal L

      2009-01-01

      Full Text Available An actinomycete strain, Streptomyces hygroscopicus subsp. ossamyceticus (strain D10 was isolated from Thar Desert soil, Rajasthan during the year 2006 and found to produce a yellow color pigment with antibiotic activity. Crude pigment was produced from strain D10 by solid state fermentation using wheat bran medium followed by extraction with ethyl acetate. The antimicrobial activity of the crude pigment was evaluated against drug resistant pathogens such as methicillin-resistant Staphylococcus aureus, vancomycin-resistant Staphylococcus aureus, extended spectrum b-lactamase producing cultures of Escherichia coli, Pseudomonas aeruginosa and Klebsiella sp. About 420 mg of crude pigment was produced per 10 g of wheat bran medium. In the disc diffusion method the crude ethyl acetate extract showed a minimum of 10 mm inhibition against Klebsiella sp. and maximum of 19 mm of inhibition against Escherichia coli. The crude pigment was partially purified using thin layer chromatography with the solvent system chloroform:methanol (30:70 and the Rf value was calculated as 0.768. Antimicrobial activity of the partially purified compound from thin layer chromatography was determined using the bioautography method. The purified pigment showed minimum of 15 mm inhibition against Klebsiella sp. and a maximum of 23 mm of inhibition against vancomycin-resistant Staphylococcus aureus in the disc diffusion method. Based on the results of chemical screening, the pigment was tentatively identified as group of sugar containing molecules.

    7. Biogenic gold nanotriangles from Saccharomonospora sp., an endophytic actinomycetes of Azadirachta indica A. Juss.

      Verma, Vijay C.; Anand, Swechha; Ulrichs, Christian; Singh, Santosh K.

      2013-04-01

      Microbial biofabrication is emerging as eco-friendly, simpler, and reproducible alternative to chemical synthesis of metals and semiconductor nanoparticles, allowing generation of rare geometrical forms such as nanotriangles and nanoprisms. Highly confined nanostructures like triangles/prisms are interesting class of nanoparticles due to their unique optical properties exploitable in biomedical diagnostics and biosensors. Here, we report for the first time a single-step biological protocol for the synthesis of gold nanotriangles using extract of endophytic actinomycetes Saccharomonospora sp., isolated from surface sterilized root tissues of Azadirachta indica A. Juss., when incubated with an aqueous solution of chloroaurate ions (AuCl- 4/1 mM). Thin, flat occasionally prismatic gold nanotriangles were produced when aqueous chloroaurate ions reacted with the cell-free extract as well as with the biomass of endophytic Saccharomonospora. It was evidenced from sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis that proteins of 42 and 50 kD were involved in biosynthesis as well as in stabilization of the nanoparticles. The particle growth process was monitored by UV-vis spectroscopy, and the morphological characterization was carried out by transmission electron microscopy and atomic force microscopy together with X-ray powder diffractions. Although the exact mechanism for this shape-oriented synthesis is not clear so far, the possibility of achieving nanoparticle shape control in a microbial system is exciting.

    8. The loop structure of Actinomycete glycoside hydrolase family 5 mannanases governs substrate recognition.

      Kumagai, Yuya; Yamashita, Keitaro; Tagami, Takayoshi; Uraji, Misugi; Wan, Kun; Okuyama, Masayuki; Yao, Min; Kimura, Atsuo; Hatanaka, Tadashi

      2015-10-01

      Endo-β-1,4-mannanases from Streptomyces thermolilacinus (StMan) and Thermobifida fusca (TfMan) demonstrated different substrate specificities. StMan hydrolyzed galactosylmannooligosaccharide (GGM5; 6(III) ,6(IV) -α-d-galactosyl mannopentaose) to GGM3 and M2, whereas TfMan hydrolyzed GGM5 to GGM4 and M1. To determine the region involved in the substrate specificity, we constructed chimeric enzymes of StMan and TfMan and evaluated their substrate specificities. Moreover, the crystal structure of the catalytic domain of StMan (StMandC) and the complex structure of the inactive mutant StE273AdC with M6 were solved at resolutions of 1.60 and 1.50 Å, respectively. Structural comparisons of StMandC and the catalytic domain of TfMan lead to the identification of a subsite around -1 in StMandC that could accommodate a galactose branch. These findings demonstrate that the two loops (loop7 and loop8) are responsible for substrate recognition in GH5 actinomycete mannanases. In particular, Trp281 in loop7 of StMan, which is located in a narrow and deep cleft, plays an important role in its affinity toward linear substrates. Asp310 in loop8 of StMan specifically bound to the galactosyl unit in the -1 subsite. PMID:26257335

    9. Degradación de tiocianato por hongos aislados de ambientes mineros y evaluación de su capacidad degradativa

      Susan Medina

      2012-10-01

      Full Text Available El Perú es uno de los principales países en la extracción de minerales como el oro, zinc, plomo y estaño. En la producción de oro se utiliza cianuro, el cual es tóxico para el medio ambiente, y que por reacción natural con el azufre se convierte en tiocianato (-SCN. En el presente trabajo se aislan hongos con capacidad de degradar tiocianato procedentes de aguas y suelos de zonas mineras de Junín y Tumbes. Estas cepas se sometieron a ensayos con concentraciones de tiocianato entre 5 y 600 mM. Asimismo, se evaluó la cinética de degradación de tiocianato en medio Kwon partiendo de 1,2 g.L-1 de KSCN y luego se realizó la identificación morfológica. De un total de 58 mohos aislados, obtuvimos 4 mohos con excelentes capacidades de degradar tiocianato y que pueden ser utilizados en biorremediación. La capacidad degradativa de estas cepas fue en promedio 10,05 mg.L-1 de -SCN con una velocidad de 28,77 mg.L-1.h-1. Dos cepas fueron identificadas fenotípica y molecularmente como Fusarium trincictum usando el marcador ITS del gen rDNA.

    10. Educational Ethics and the DESD: Considering Trade-Offs

      Schlottmann, Christopher

      2008-01-01

      The United Nation's Decade of Education for Sustainable Development (DESD) aims to prepare students for pressing economic and environmental problems. In this article, I argue that an exclusive emphasis on an ambiguous goal, sustainable development, raises important questions for educational ethics. Specifically, I argue that DESD mission…

    11. Desde el proyecto 6x4

      Diana Soto Arango

      2007-01-01

      Full Text Available El Proyecto 6X4 tiene por objeto estudiar seis profesiones desde cuatro ejes de análisis bajo los lineamientos del denominado Proceso de Bolonia de la Unión Europea, en este caso, en concordancia con América Latina y el Caribe. En el proyecto general participan 60 universidades de América Latina y de Europa. En nuestro continente se localizan 12 países y actualmente, la dirección del Proyecto 6X4 está liderada por ASCUN en Colombia. Este trabajo presenta la situación actual en la formación de la carrera de “Historia” y de Ciencias Sociales en pregrado y postgrado, con el eje de análisis de “investigación e innovación” en el citado Proyecto 6X4. Específicamente, se presentan las competencias en el citado eje de análisis. Al final, se toma la experiencia de RUDECOLOMBIA, para replantear la formación en Maestrías y Doctorados. dentro de esta Red académica.

    12. Phytoactinopolyspora alkaliphila sp. nov., an alkaliphilic actinomycete isolated from a saline-alkaline soil.

      Zhang, Yong-Guang; Lu, Xin-Hua; Ding, Yan-Bo; Zhou, Xing-Kui; Li, Li; Guo, Jian-Wei; Wang, Hong-Fei; Duan, Yan-Qing; Li, Wen-Jun

      2016-05-01

      An alkaliphilic, filamentous actinomycete, designated EGI 80629T, was isolated from a soil sample of Xinjiang, north-west China. Strain EGI 80629T grew at pH 6.0-11.0 (optimum pH 9.0-10.0) and in the presence of 0-13.0 % NaCl (optimum 3.0-5.0 %). The isolate formed fragmented substrate mycelia, and aerial hyphae with short spore chains with rod-like spores. Whole-cell hydrolysates of the isolate contained ll-diaminopimelic acid as the diagnostic diamino acid, and mannose and rhamnose as diagnostic sugars. The major fatty acids identified were iso-C15 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C17 : 0. The predominant menaquinone was MK-9(H4), while the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two phosphatidylinositol mannosides, five unknown phospholipids, three unknown phosphoglycolipids, one unknown glycolipid, four unknown polar lipids and one unknown aminophospholipid. The G+C content of the genomic DNA was 67.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain EGI 80629T clustered with the genus Phytoactinopolyspora. The 16S rRNA gene sequence similarity between strain EGI 80629T and Phytoactinopolyspora endophytica EGI 60009T was 96.8 %. Based on morphological, chemotaxonomic and phylogenetic characteristics, strain EGI 80629T represents a novel species of the genus Phytoactinopolyspora, for which the name Phytoactinopolyspora alkaliphila sp. nov. is proposed. The type strain is EGI 80629T ( = CGMCC 4.7225T = KCTC 39701T). PMID:26920762

    13. Diketopiperazine Derivatives from the Marine-Derived Actinomycete Streptomyces sp. FXJ7.328

      Weiming Zhu

      2013-03-01

      Full Text Available Five new diketopiperazine derivatives, (3Z,6E-1-N-methyl-3-benzy lidene-6-(2S-methyl-3-hydroxypropylidenepiperazine-2,5-dione (1, (3Z,6E-1-N-methyl-3-benzylidene-6-(2R-methyl-3-hydroxypropylidenepiperazine-2,5-dione (2, (3Z,6Z-3- (4-hydroxybenzylidene-6-isobutylidenepiperazine-2,5-dione (3, (3Z,6Z-3-((1H-imidazol-5-yl-methylene-6-isobutylidenepiperazine-2,5-dione (4, and (3Z,6S-3-benzylidene-6-(2S-but-2-ylpiperazine-2,5-dione (5, were isolated from the marine-derived actinomycete Streptomyces sp. FXJ7.328. The structures of 1–5 were determined by spectroscopic analysis, CD exciton chirality, the modified Mosher’s, Marfey’s and the C3 Marfey’s methods. Compound 3 showed modest antivirus activity against influenza A (H1N1 virus with an IC50 value of 41.5 ± 4.5 μM. In addition, compound 6 and 7 displayed potent anti-H1N1 activity with IC50 value of 28.9 ± 2.2 and 6.8 ± 1.5 μM, respectively. Due to the lack of corresponding data in the literature, the 13C NMR data of (3Z,6S-3-benzylidene-6-isobutylpiperazine-2,5-dione (6 were also reported here for the first time.

    14. Rare actinomycetes Nocardia caishijiensis and Pseudonocardia carboxydivorans as endophytes, their bioactivity and metabolites evaluation.

      Tanvir, Rabia; Sajid, Imran; Hasnain, Shahida; Kulik, Andreas; Grond, Stephanie

      2016-04-01

      Two strains identified as Nocardia caishijiensis (SORS 64b) and Pseudonocardia carboxydivorans (AGLS 2) were isolated as endophytes from Sonchus oleraceus and Ageratum conyzoides respectively. The analysis of their extracts revealed them to be strongly bioactive. The N. caishijiensis extract gave an LC50 of 570 μg/ml(-1) in the brine shrimp cytotoxicity assay and an EC50 of 0.552 μg/ml(-1) in the DPPH antioxidant assay. Antimicrobial activity was observed against Methicillin resistant Staphlococcus aureus (MRSA) and Escherichia coli ATCC 25922 (14 mm), Klebsiella pneumoniae ATCC 706003 (13 mm), S. aureus ATCC 25923 (11 mm) and Candida tropicalis (20 mm). For the extract of P. carboxydivorans the EC50 was 0.670 μg/ml(-1) and it was observed to be more bioactive against Bacillus subtilis DSM 10 ATCC 6051 (21 mm), C. tropicalis (20 mm), S. aureus ATCC 25923 (17 mm), MRSA (17 mm), E. coli K12 (W1130) (16 mm) and Chlorella vulgaris (10 mm). The genotoxicity testing revealed a 20 mm zone of inhibition against the polA mutant strain E. coli K-12 AB 3027 suggesting damage to the DNA and polA genes. The TLC and bioautography screening revealed a diversity of active bands of medium polar and nonpolar compounds. Metabolite analysis by HPLC-DAD via UV/vis spectral screening suggested the possibility of stenothricin and bagremycin A in the mycelium extract of N. caishijiensis respectively. In the broth and mycelium extract of P. carboxydivorans borrelidin was suggested along with α-pyrone. The HPLC-MS revealed bioactive long chained amide derivatives such as 7-Octadecenamide, 9, 12 octadecandienamide. This study reports the rare actinomycetes N. caishijiensis and P. carboxydivorans as endophytes and evaluates their bioactive metabolites. PMID:26946375

    15. Streptomyces formicae sp. nov., a novel actinomycete isolated from the head of Camponotus japonicus Mayr.

      Bai, Lu; Liu, Chongxi; Guo, Lifeng; Piao, Chenyu; Li, Zhilei; Li, Jiansong; Jia, Feiyu; Wang, Xiangjing; Xiang, Wensheng

      2016-02-01

      During a screening for novel and biotechnologically useful actinobacteria in insects, a novel actinomycete with antifungal activity, designated strain 1H-GS9(T), was isolated from the head of a Camponotus japonicus Mayr ant, which were collected from Northeast Agricultural University (Harbin, Heilongjiang, China). Strain 1H-GS9(T) was characterised using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of members of the genus Streptomyces. 16S rRNA gene sequence similarity studies showed that strain 1H-GS9(T) belongs to the genus Streptomyces with high sequence similarities to Streptomyces scopuliridis DSM 41917(T) (98.8 %) and Streptomyces mauvecolor JCM 5002(T) (98.6 %). However, phylogenetic analysis based on the 16S rRNA gene sequence indicated that it forms a monophyletic clade with Streptomyces kurssanovii JCM 4388(T) (98.6 %), Streptomyces xantholiticus JCM 4282(T) (98.6 %) and Streptomyces peucetius JCM 9920(T) (98.5 %). Thus, a combination of DNA-DNA hybridization experiments and phenotypic tests were carried out between strain 1H-GS9(T) and the above-mentioned five strains, which further clarified their relatedness and demonstrated that strain 1H-GS9(T) could be distinguished from these strains. Therefore, the strain is concluded to represent a novel species of the genus Streptomyces, for which the name Streptomyces formicae sp. nov. is proposed. The type strain is 1H-GS9(T) (=CGMCC 4.7277(T) = DSM 100524(T)). PMID:26608172

    16. In Vitro and In Vivo Plant Growth Promoting Activities and DNA Fingerprinting of Antagonistic Endophytic Actinomycetes Associates with Medicinal Plants.

      Ajit Kumar Passari

      Full Text Available Endophytic actinomycetes have shown unique plant growth promoting as well as antagonistic activity against fungal phytopathogens. In the present study forty-two endophytic actinomycetes recovered from medicinal plants were evaluated for their antagonistic potential and plant growth-promoting abilities. Twenty-two isolates which showed the inhibitory activity against at least one pathogen were subsequently tested for their plant-growth promoting activities and were compared genotypically using DNA based fingerprinting, including enterobacterial repetitive intergenic consensus (ERIC and BOX repetitive elements. Genetic relatedness based on both ERIC and BOX-PCR generates specific patterns corresponding to particular genotypes. Exponentially grown antagonistic isolates were used to evaluate phosphate solubilization, siderophores, HCN, ammonia, chitinase, indole-3-acetic acid production, as well as antifungal activities. Out of 22 isolates, the amount of indole-3-acetic acid (IAA ranging between 10-32 μg/ml was produced by 20 isolates and all isolates were positive for ammonia production ranging between 5.2 to 54 mg/ml. Among 22 isolates tested, the amount of hydroxamate-type siderophores were produced by 16 isolates ranging between 5.2 to 36.4 μg/ml, while catechols-type siderophores produced by 5 isolates ranging from 3.2 to 5.4 μg/ml. Fourteen isolates showed the solubilisation of inorganic phosphorous ranging from 3.2 to 32.6 mg/100ml. Chitinase and HCN production was shown by 19 and 15 different isolates, respectively. In addition, genes of indole acetic acid (iaaM and chitinase (chiC were successively amplified from 20 and 19 isolates respectively. The two potential strains Streptomyces sp. (BPSAC34 and Leifsonia xyli (BPSAC24 were tested in vivo and improved a range of growth parameters in chilli (Capsicum annuum L. under greenhouse conditions. This study is the first published report that actinomycetes can be isolated as endophytes from

    17. Isolation, screening and characterization of uranium microremediable actinomycetes from fallen leaves of Azadirachta indica in Western Ghats

      Microremediation of harmful radioactive waste such as uranium has been carried out by the endophytic actinomycetes strains isolated from the unnoticed fallen leaves of commonly available medicinal plant Azadirachta indica, which are considered as unique source. Among six actinobacteria isolates, one microbe (A5) effectively removed uranium in 12 h at temperature 30 deg C and pH 8-9. Molecular characterization and phylogenetic analysis support the classification of the isolate A5 as a new strain which was named as Streptomyces sp. MINIYAA7 (Genbank accession number KF909129). (author)

    18. Complete genome sequence of Kibdelosporangium phytohabitans KLBMP 1111(T), a plant growth promoting endophytic actinomycete isolated from oil-seed plant Jatropha curcas L.

      Qin, Sheng; Feng, Wei-Wei; Xing, Ke; Bai, Juan-Luan; Yuan, Bo; Liu, Wei-Jie; Jiang, Ji-Hong

      2015-12-20

      Kibdelosporangium phytohabitans KLBMP 1111(T) is a plant growth promoting endophytic actinomycete isolated from the oil-seed plant Jatropha curcas L. collected from dry-hot valley, in Sichuan, China. The complete genome sequence of this actinomycete consists of one chromosome (11,759,770bp) with no plasmid. From the genome, we identified gene clusters responsible for polyketide and nonribosomal peptide synthesis of natural products, and genes related to the plant growth promoting, such as zeatin, 1-aminocyclopropane-1-carboxylate deaminase (ACCD) and siderophore. The complete genome information may be useful to understand the beneficial interactions between K. phytohabitans KLBMP 1111(T) and host plants. PMID:26516119

    19. Dinámica poblacional del rotífero Brachionus ibericus aislado de estanques para camarón, alimentado con diferentes dietas

      José Cristóbal Román-Reyes

      2014-11-01

      Full Text Available La producción de microalgas vivas para cultivar rotíferos constituye uno de los mayores costos de operación en la larvicultura de peces, por lo que se están desarrollando sustitutos comerciales de microalgas para la alimentación y producción de rotíferos. Se desconoce el efecto que tienen las formulaciones comerciales sobre la dinámica poblacional de rotíferos nativos del noroeste de México y en este estudio se evaluó el efecto de cuatro dietas comerciales (Espirulina, RotiMac®, Nanno 3600®, RotiGrow-Plus® y la microalga viva Nannochloropsis sp., sobre el crecimiento poblacional y fecundidad del rotífero Brachionus ibericus (GenBank KJ949043, aislado de una granja de camarón blanco (Litopenaeus vannamei. Se realizaron cinco réplicas por tratamiento alimenticio utilizando recipientes con volumen de 12 L de agua, con temperatura de 29 ± 1°C y salinidad de 35 ± 1, respectivamente. Los resultados mostraron que durante los periodos experimentales, las dietas probadas resultaron adecuadas para alimentar a B. ibericus, con tasas de crecimiento, tiempos de duplicación, densidades máximas y fecundidades que fluctuaron entre 0.20 a 0.27 rotíferos día-1, 2.60 a 3.42 días, 215 a 344 rotíferos mL-1 y de 0.16 a 0.39 huevos hembra-1, respectivamente. Las diferencias entre tratamientos fueron significativas (P 0.05 a las obtenidas con Nanno 3600®.

    20. Caracterización de aislados de rizobios provenientes de nódulos de soya (Glycine max (L.) Merril) con potencialidades en la promoción del crecimiento vegetal

      Ionel Hernández Forte; María C. Nápoles García; Belkis Morales Mena

      2015-01-01

      El objetivo del presente trabajo fue caracterizar cuatro aislados de rizobios y la cepa Bradyrhizobium elkanii ICA 8001 provenientes de nódulos de plantas de soya, en cuanto a su capacidad para exhibir tres atributos positivos que caracterizan estas rizobacterias promotoras del crecimiento vegetal. Se determinó su capacidad de producir sideróforos; AIA (ácido 3-indolacético) y la actividad específica de la enzima 1-aminociclopropano-1- ácido carboxílico (ACC) desaminasa. Solo el aisl...

    1. Generación eléctrica mediante un sistema híbrido hidráulico-fotovoltaico aislado de la red para una pequeña población rural

      Visiga Delgado, Daniel

      2009-01-01

      El objeto del proyecto es el diseño de un sistema, factible tecnológicamente, híbrido hidráulicofotovoltaico, aislado de la red para una pequeña población rural. Este sistema está dimensionado a partir de un río tipo y recibe el apoyo de un sistema fotovoltaico y un tanque de baterías. Por otro lado, el proyecto tiene la finalidad de crear una demanda escalable unitaria base para una vivienda rural tipo y, a partir de la cual, obtener la demanda de la población.

    2. Evaluación de la ampliación de ALUAR con el SIP aislado e interconectado al SADI : Parte 1: aspectos generales y estabilidad dinámica

      Agüero, Jorge Luis; Beroqui, Mario César; Barbieri, María Beatriz; Macchione, Luis; Cosatti, Oscar

      2005-01-01

      Se presenta una descripción de los tipos de estudios realizados, modelos utilizados y simulaciones de comportamiento efectuados para evaluar el efecto de la ampliación de la planta de Aluar sobre el SIP aislado y sobre el SADI-SIP interconectados. El objetivo de los estudios es la definición básica de las nuevas instalaciones, la adecuación de las existentes, la definición del equipamiento de control y los recursos estabilizantes requeridos, y la posible modificación de los recursos post f...

    3. Caracterización de especies y perfil de resistencia antimicrobiana en enterococos aislados de alimentos de origen animal provenientes de un área rural del centro de la provincia de Buenos Aires, Argentina

      Delpech, Gastón

      2014-01-01

      El objetivo general de esta investigación fue caracterizar fenotípicamente y determinar el perfil de resistencia in vitro de especies de enterococos aislados de alimentos de origen animal provenientes de un área rural del Centro de la Provincia de Buenos Aires, Argentina. Durante el período 2009-2012 se analizaron N = 1937 muestras de carne picada (n = 1080), salamines artesanales (n = 642), quesos de vaca (n = 119), quesos de cabra (n = 42), leche de cabra (n = 30) y queso de oveja (n = 2...

    4. Mantenimiento en cultivo y caracterización de un microsporidio (Encephalitozoon hellem aislado en un paciente con Sida y neumonía MANTAINED IN CULTURE AND CHARACTERIZATION OF A MICROSPORIDIAN (Encephalitozoon hellem ISOLATED FROM AIDS PATIENT WITH NEUMONIA

      FERNANDO J. BORNAY-LLINARES

      2000-07-01

      Full Text Available Comunicamos la identificación, a nivel de especie, de un microsporidio aislado en cultivo celular a partir de un lavado broncoalveolar de un paciente con Sida y neumonía. La caracterización del aislado se realizó mediante: 1 estudio morfológico utilizando métodos de microscopía óptica y electrónica, 2 estudio inmunológico con antisueros específicos, inmunofluorescencia indirecta (IFI e inmunoblot (WB y 3 estudio molecular tras reacción en cadena de la polimerasa (PCR con iniciadores especie-específicos diseñados a partir de la región que codifica la subunidad menor del ARN ribosomal. Las características ultraestructurales del aislado permitieron su identificación en el género Encephalitozoon. La identificación específica del microsporidio como Encephalito-zoon hellem se realizó mediante IFI y WB, empleando suero policlonal de conejo anti-E. hellem (CDC:0291:V213, y mediante la amplificación por PCR del fragmento diagnóstico utilizando el par de iniciadores EHELF/EHELR específicos para esta especie. El aislado ha sido denominado EHVS-96 y se mantiene en cultivo continuo en células Vero-E6. Este es el primer aislamiento en cultivo y caracterización de E. hellem en España.We report the identification to the species level of a microsporidian isolated from the bronchoalveolar lavage of an AIDS patient in Spain. Characterization of the isolate was performed by: 1 morphological methods using optical and electron microscopy; 2 immunological methods such as immunofluorescence assay (IIF and immunoblot (WB using specific antiserum and, 3 polymerase chain reaction (PCR using species-specific primers designed on the coding region of the small subunit of the ribosomal RNA gene. Ultrastructural features allowed the identification of the isolate as belonging to the Encephalitozoon genus. Species identification as Encephalitozoon hellem was achieved by IIF and WB using polyc lonal rabbit serum anti-E. hellem (CDC:0291:V213, as well as

    5. Cytotoxicity of actinomycetes associated with the ascidian Eudistoma vannamei (Millar, 1977, endemic of northeastern coast of Brazil

      Paula C Jimenez

      2013-04-01

      Full Text Available Previous studies demonstrated that the crude extract of the ascidian Eudistoma vannamei, endemic from northeasttern Brazil, strongly hinders growth of tumor cells in vitro by inducing apoptosis due to tryptophan derivatives, which are commonly found in bacteria. This study presents a bioactivity-guided screening among actinomycetes, associated with E. vannamei, aiming at recognizing active principles with biological relevance. Twenty strains of actinomycetes, designated as EVA 0101 through 0120, were isolated from colonies of E. vannamei among which 11 were selected for cytotoxicity evaluation. The extracts from EVA 0102, 0103, 0106, 0109 and 0113 were the most active, and were further studied for IC50 determination and chemical analysis by ¹H NMR. IC50 values obtained ranged from 3.62 µg mL-1 (for EVA 0109 in leukemia cells to 84.65 µg/mL (for EVA 0106 in melanoma cells. All active extracts exhibited the same TLC and spectroscopic profiles, suggesting the presence of quinones and other related secondary metabolites. Furthermore, these strains were identified and compared based on their respective 16S rRNA sequences. The results herein identified the five strains as Micromonospora spp. while phylogenetic analysis suggests that they are possibly two different Micromonospora species producing the cytotoxic compounds.

    6. Saccharopolyspora subtropica sp. nov., a thermophilic actinomycete isolated from soil of a sugar cane field.

      Wu, Hao; Liu, Bin; Pan, Shangli

      2016-05-01

      A novel thermophilic actinomycete, designated strain T3T, was isolated from a soil sample of a sugar cane field. The strain grew at 25-60 °C (optimum 37-50 °C), at pH 6.0-11.0 (optimum 7.0-9.0) and with 0-12.0 % (w/v) NaCl (optimum 0-7 %). The aerial mycelium was white and the vegetative mycelium was colourless to pale yellow. The substrate mycelium fragmented into rod-shaped elements after 4-5 days at 50 °C. The aerial mycelium formed flexuous chains of 5-20 spores per chain; the oval-shaped spores had spiny surfaces and were non-motile. The organism contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars consisted of arabinose, galactose and ribose. The cellular fatty acid profile consisted mainly of anteiso-C17 : 0, iso-C17 : 0 and iso-C16 : 0. The quinone system was composed predominantly of MK-9(H4). The phospholipids detected were diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, phosphatidylmethylethanolamine and ninhydrin-positive glycophospholipids. The DNA G+C content of strain T3T was 71.3 mol%. The organism showed a combination of morphological and chemotaxonomic properties typical of members of the genus Saccharopolyspora. In the 16S rRNA gene tree of Saccharopolyspora it formed a distinct phyletic line and was related most closely to Saccharopolyspora thermophila 216T. However, the phenotypic characteristics of strain T3T were significantly different from those of S. thermophila 216T and DNA-DNA hybridization revealed a low level of relatedness (28.6-32.3 %) between them. Based on the phenotypic and phylogenetic data, strain T3T represents a novel species in the genus Saccharopolyspora, for which the name Saccharopolyspora subtropica sp. nov. is proposed. The type strain is T3T ( = DSM 46801T = CGMCC 4.7206T). PMID:26882893

    7. XML In Vitro Comparison of MIC Crude Extracts of Active Actinomycetes Isolated with Terbinafine, Griseofulvin Ketoconazole and Fluconazole against Microsporum Canis, Microsporum Gypseum and Trichophyton Mentagrophytes

      Keikha, N. (MSc

      2015-05-01

      Full Text Available Background and Objective: Dermatophytes are the fungi that have the ability to attack the keratinized tissues such as the skin, hair and nails. Infections caused by these organisms are named dermatophytosis. We aimed to compare Minimum inhibitory concentration (MIC of Crude extracts of Active Actinomycete Isolates with Terbinafine, Griseofulvin, Ketoconazole and Fluconazole Drugs against Microsporum Canis, Microsporum gypseum and Trichophyton mentagrophytes. Material and Methods: In this experimental study, in order to find MIC by actionmycete, 100 isolates were studied and then crude extracts of the active actinomycete isolates were prepared in sterile conditions. Finally, the crude extracts obtained at different concentrations were used to obtain the MIC of Microsporum Canis, Microsporum gypseum and Trichophyton mentagrophytes. Moreover, various concentrations of the drugs such as terbinafine, griseofulvin, ketoconazole and fluconazole in solvent Dimethyl sulfoxide (DMSO were prepared and their growth inhibitory effect was evaluated and then compared with the results obtained from the crude extract of active actinomycete isolates. Results: the crude extracts obtained from active Actioiomycetes isolates and the drugs such as terbinafine, griseofulvin, ketoconazole and fluconazole, in a dose-dependent manner, could inhibit the growth of Microsporum Canis, Microsporum gypseum and Trichophyton Mentagrophytes. Conclusion: compared to MIC of Crude extract of active actinomycete isolates, Terbinafine has a significant effect on the growth inhibition in all of the fungal Dermatophytes and then griseofulvin, ketoconazole and fluconazole are in the next rank, respectively.

    8. CCACB - Culture Collection of Actinomycetes of the Institute of Soil Biology České Budějovice, Czech Republic

      Krištůfek, Václav; Chroňáková, Alica; Němec, Jan

      Osnabrück: University Osnabrück, 2009. s. 137. [Biology of Streptomycetes. 07.10.2009-11.10.2009, Münster] Institutional research plan: CEZ:AV0Z60660521 Keywords : Culture Collection of Actinomycetes * Institute of Soil Biology * České Budějovice Subject RIV: EH - Ecology, Behaviour

    9. CCACB - Culture Collection of Actinomycetes of the Institute of Soil Biology České Budějovice, Czech Republic

      Krištůfek, Václav; Chroňáková, Alica; Petrásek, Jiří; Chrudimský, Tomáš; Elhottová, Dana

      Praha: Institute of Chemical Technology Prague, 2014. s. 157-158. ISBN 978-80-7080-887-0. [BioTech 2014 & 6 th Czech-Swiss Symposium with Exhibition. 11.06.2014-14.06.2014, Praha] Institutional support: RVO:60077344 Keywords : CCACB * soil actinomycetes * depository Subject RIV: EE - Microbiology, Virology

    10. Complete Genome Sequence of Micromonospora Strain L5, a Potential Plant-Growth-Regulating Actinomycete, Originally Isolated from Casuarina equisetifolia Root Nodules

      Hirsch, A. M.; Alvarado, J.; Bruce, D.; Chertkov, O.; De Hoff, P. L.; Detter, J. C.; Fujishige, N. A.; Goodwin, L. A.; Han, J.; Han, S.; Ivanova, N.; Land, M. L.; Lum, M. R.; Milani-Nejad, N.; Nolan, M.; Pati, A.; Pitluck, S.; Tran, S. S.; Woyke, T.; Valdes, M.

      2013-08-29

      Micromonospora species live in diverse environments and exhibit a broad range of functions including antibiotic production, biocontrol, and ability to degrade complex polysaccharides. To learn more about these versatile actinomycetes, we sequenced the genome of strain L5, originally isolated from root nodules of an actinorhizal plant growing in Mexico.

    11. Comparative analysis of oligonucleotide primers for high-throughput screening of genes encoding adenylation domains of nonribosomal peptide synthetases in actinomycetes

      Bakal, Tomáš; Goo, K.-S.; Najmanová, Lucie; Plháčková, Kamila; Kadlčík, Stanislav; Ulanová, Dana

      2015-01-01

      Roč. 108, č. 5 (2015), s. 1267-1274. ISSN 0003-6072 R&D Projects: GA MŠk(CZ) ED1.1.00/02.0109 Institutional support: RVO:61388971 Keywords : Nonribosomal peptide synthetase * Adenylation domain * Actinomycetes Subject RIV: EE - Microbiology, Virology Impact factor: 1.806, year: 2014

    12. Evaluating Susceptibility to Commercial Fungicide of Endophytic Fungi Isolated from Roses (Rosa hybrida Evaluación de la susceptibilidad de hongos endófitos aislados de rosa (rosa hybrida a fungicidas comerciales

      Restrepo Silvia

      2007-07-01

      ófitos.    En este trabajo se evaluó la susceptibilidad de hongos endófitos aislados de Rosa hybrida a fungicidas utilizados comercialmente en el control de patógenos en el cultivo de rosa. Esto se realizó in vitro, mezclando diferentes concentraciones de fungicidas con medios estándares en el crecimiento de hongos endófitos y midiendo diariamente su crecimiento.  La susceptibilidad de Botrytis cinerea (Cepa 3015, uno de los más importantes patógenos que afecta el cultivo de rosas en Colombia, se analizó de la misma forma. El 45,45% de los hongos endófitos evaluados demostraron susceptibilidad de crecimiento con grados de sensibilidad desde no sensibles (³73,75% hasta regularmente sensibles (³48,75% - 61,25% en las concentraciones evaluadas principalmente en fungicidas como boscalid, captan, iprodione y pyrimethanil. En el caso de fungicidas como carboxin más thiram, fludioxonil más ciprodinil, y prochloraz, se observaron grados de susceptibilidad de alta sensibilidad (23,75%, inhibiendo totalmente el crecimiento de los hongos endófitos evaluados. En B. cinerea (Cepa 3015 se observó alta susceptibilidad a pyrimethanil, carboxin más thiram, fludioxonil más ciprodinil, y prochloraz. Aunque los controles de B. cinerea tuvieron mayores crecimientos, la mayoría de los crecimientos de los hongos endófitos evaluados en los medios enmendados en las dos concentraciones fueron superiores a los de este patógeno. El rango de susceptibilidad de hongos endófitos como las cepas 3002, 3003, 3004, 3005 y 3006 bajo los parámetros de análisis de este experimento, muestra su selección como hongos promisorios para programas de manejo integrado de plagas y enfermedades, teniendo en cuenta el momento, la frecuencia y la dosis de aplicación tanto de los fungicidas como de los hongos endófitos empleados.

    13. Contribución al conocimiento de la biología y taxonomía de un hongo del género Phytium aislado del "aliso", Alnus acuminata HBK Contribución al conocimiento de la biología y taxonomía de un hongo del género Phytium aislado del "aliso", Alnus acuminata HBK

      Cabrera Orlando

      1990-06-01

      Full Text Available

      Se estudio la biología de un hongo aislado de la parte externa de nódulos de raíces de aliso (Alnus acumi"ata HB K, el cual presento un alto poder de germinación a bajas temperaturas (entre QOC y 4QC, condición que le confiere gran agresividad para colonizar el suelo, de tal forma que se constituye en un factor importante en la ecofisiología de las poblaciones de aliso que crecen en los bosques secundarios del sub-paramo. A partir de cultivos puros se hicieron estudios para su identificación.

      Some biological aspects of an isolated fungus of Alnus acuminata roots were studied. It bas been found Tbat between OOC and 4°C tbe fungi grows fast. This condition can result in a important factor in the ecophysiology of 'Aliso' populations, that grows in secondary forests at sub-paramo, After a review of the taxonomic literature, we placed it in the Phytium genus, but not in a particular described species. We assume that it is a new species. We modificated the microculture technique that was simpler and safer and them we made direct observations about tbe fungus growtb and development. As for now it is nominated as Phytiun near Mammillatum Meurs. Palabras Claves: Phytium, bongos, A/nus acuminata HBK., "Aliso".

    14. Equidad y politicas universitarias: perspectivas desde latinoamerica

      José Alberto Yuni

      2014-08-01

      Full Text Available En este artículo nos proponemos abordar algunas cuestiones de la equidad en la Educación Superior, desde la singular perspectiva de la realidad latinoamericana. Nuestro interés radica en dar cuenta de los modos y posiciones teóricas a través de las cuales se definen políticas de equidad en el campo de la Educación Superior y se establecen sentidos y significados sociales en torno a ellas. En la primera parte se realiza una breve caracterización de los escenarios de la Educación Superior en Latinoamérica, tanto en lo que se refiere a los procesos socio-políticos de carácter estructural, como a la dinámica de los sistemas de Educación Superior. En la segunda parte, se efectúa una reconstrucción conceptual que nos permita mostrar los desplazamientos operados en las políticas universitarias de las últimas décadas y que han desembocado en la implementación de las llamadas políticas de equidad. En la tercera parte del artículo, se analizan diferentes niveles de especificación del concepto de equidad en las dinámicas universitarias, las que dan cuenta de su potencial productivo de prácticas. Finalmente, se describen algunas de las iniciativas más importantes desplegadas en los últimos años por algunos países de la región, enmarcadas en políticas de equidad, señalando sus efectos y alcances.ABSTRACTIn this article we aim to address some issues of equity in higher education, from the particular perspective of Latin American reality. Our interest is highlight the fact of the theoretical and methodological positions through which equity policies are defined in the field of higher education and how social meanings and ways are established around them. In the first part, a brief description of the scenarios of higher education in Latin America is done, both in regard to the socio-political structural processes and the dynamics of higher education systems. In the second part, a conceptual reconstruction is approached to allow

    15. Metagenomic of Actinomycetes Based on 16S rRNA and nifH Genes in Soil and Roots of Four Indonesian Rice Cultivars Using PCR-DGGE

      Mahyarudin

      2015-07-01

      Full Text Available The research was conducted to study the metagenomic of actinomycetes based on 16S ribosomal RNA (rRNA and bacterial nifH genes in soil and roots of four rice cultivars. The denaturing gradient gel electrophoresis profile based on 16S rRNA gene showed that the diversity of actinomycetes in roots was higher than soil samples. The profile also showed that the diversity of actinomycetes was similar in four varieties of rice plant and three types of agroecosystem. The profile was partially sequenced and compared to GenBank database indicating their identity with closely related microbes. The blast results showed that 17 bands were closely related ranging from 93% to 100% of maximum identity with five genera of actinomycetes, which is Geodermatophilus, Actinokineospora, Actinoplanes, Streptomyces and Kocuria. Our study found that Streptomyces species in soil and roots of rice plants were more varied than other genera, with a dominance of Streptomyces alboniger and Streptomyces acidiscabies in almost all the samples. Bacterial community analyses based on nifH gene denaturing gradient gel electrophoresis showed that diversity of bacteria in soils which have nifH gene was higher than that in rice plant roots. The profile also showed that the diversity of those bacteria was similar in four varieties of rice plant and three types of agroecosystem. Five bands were closely related with nifH gene from uncultured bacterium clone J50, uncultured bacterium clone clod-38, and uncultured bacterium clone BG2.37 with maximum identity 99%, 98%, and 92%, respectively. The diversity analysis based on 16S rRNA gene differed from nifH gene and may not correlate with each other. The findings indicated the diversity of actinomycetes and several bacterial genomes analyzed here have an ability to fix nitrogen in soil and roots of rice plant.

    16. Isolation, abundance and phylogenetic affiliation of endophytic actinomycetes associated with medicinal plants and screening for their in vitro antimicrobial biosynthetic potential.

      Passari, Ajit K; Mishra, Vineet K; Saikia, Ratul; Gupta, Vijai K; Singh, Bhim P

      2015-01-01

      Microorganisms associated with medicinal plants are of interest as the producers of important bioactive compounds. To date, the diversity of culturable endophytic actinomycetes associated with medicinal plants is in its initial phase of exploration. In this study, 42 endophytic actinomycetes were isolated from different organs of seven selected medicinal plants. The highest number of isolates (n = 22, 52.3%) of actinomycetes was isolated from roots, followed by stems (n = 9, 21.4%), leaves (n = 6, 14.2%), flowers (n = 3, 7.1%), and petioles (n = 2, 4.7%). The genus Streptomyces was the most dominant among the isolates (66.6%) in both the locations (Dampa TRF and Phawngpuii NP, Mizoram, India). From a total of 42 isolates, 22 isolates were selected for further studies based on their ability to inhibit one of the tested human bacterial or fungal pathogen. Selected isolates were identified based on 16S rRNA gene analysis and subsequently the isolates were grouped to four different genera; Streptomyces, Brevibacterium, Microbacterium, and Leifsonia. Antibiotic sensitivity assay was performed to understand the responsible antimicrobials present in the isolates showing the antimicrobial activities and revealed that the isolates were mostly resistant to penicillin G and ampicillin. Further, antimicrobial properties and antibiotic sensitivity assay in combination with the results of amplification of biosynthetic genes polyketide synthase (PKS-I) and non-ribosomal peptide synthetase (NRPS) showed that the endophytic actinomycetes associated with the selected medicinal plants have broad-spectrum antimicrobial activity. This is the first report of the isolation of Brevibacterium sp., Microbacterium sp., and Leifsonia xyli from endophytic environments of medicinal plants, Mirabilis jalapa and Clerodendrum colebrookianum. Our results emphasize that endophytic actinomycetes associated with medicinal plants are an unexplored resource for the discovery of biologically active

    17. Isolation, abundance and phylogenetic affiliation of endophytic actinomycetes associated with medicinal plants and screening for their in vitro antimicrobial biosynthetic potential

      Ajit Kumar Passari

      2015-04-01

      Full Text Available Microorganisms associated with medicinal plants are of interest as the producers of important bioactive compounds. To date, the diversity of culturable endophytic actinomycetes associated with medicinal plants is in its initial phase of exploration. In this study, 42 endophytic actinomycetes were isolated from different organs of seven selected medicinal plants. The highest number of isolates (n=22, 52.3% of actinomycetes was isolated from roots, followed by stems (n=9, 21.4%, leaves (n=6, 14.2%, flowers (n=3, 7.1% and petioles (n=2, 4.7%. The genus Streptomyces was the most dominant among the isolates (66.6% in both the locations (Dampa TRF and Phawngpuii NP, Mizoram, India. From a total of 42 isolates, 22 isolates were selected for further studies based on their ability to inhibit one of the tested human bacterial or fungal pathogen. Selected isolates were identified based on 16S rRNA gene analysis and subsequently the isolates were grouped to four different genera; Streptomyces, Brevibacterium, Microbacterium and Leifsonia. Antibiotic sensitivity assay was performed to understand the responsible antimicrobials present in the isolates showing the antimicrobial activities and revealed that the isolates were mostly resistant to penicillin G and ampicillin. Further, antimicrobial properties and antibiotic sensitivity assay in combination with the results of amplification of biosynthetic genes polyketide synthase (PKS-I and nonribosomal peptide synthetase (NRPS showed that the endophytic actinomycetes associated with the selected medicinal plants have broad-spectrum antimicrobial activity. This is the first report of the isolation of Brevibacterium sp., Microbacterium sp. and Leifsonia xyli from endophytic environments of medicinal plants, Mirabilis jalapa and Clerodendrum colebrookianum. Our results emphasize that endophytic actinomycetes associated with medicinal plants are an unexplored resource for the discovery of biologically active

    18. Cultivable actinomycetes from rhizosphere of birch (Betula pendula) growing on a coal mine dump in Silets, Ukraine.

      Ostash, Bohdan; Gren, Tetiana; Hrubskyy, Yaroslav; Tistechok, Stepan; Beshley, Stepan; Baranov, Volodymyr; Fedorenko, Victor

      2014-08-01

      Five actinomycete strains were isolated from the rhizosphere of birch, one of a few native tree forms capable of thriving on the upper level of a coal mine dump near the village of Silets (Lvivska region, Ukraine). No such strains were isolated from surrounding gangue, or from nearby grass Calamagrostis epigeios. Using 16S rDNA sequencing and analysis of cell wall aminoacids, four of these strains were shown to belong to genus Streptomyces and one to be Amycolatopsis. The isolates were able to produce siderophores and antibacterial compounds. In comparison to the reference strain Streptomyces coelicolor M145, certain rhizospheric isolates displayed somewhat increased survival in the presence of copper, iron(III), or chromium(VI) salts. The Amycolatopsis isolate was also shown to accumulate significant quantities of heavy metals from waste extracts. Possible roles of the described strains in coal mine dump ecology are discussed. PMID:23686352

    19. Enfermería desde una unidad de conductas adictivas

      Santiago Forcada

      2004-01-01

      La unidad de conductas adictivas es un recurso asistencial para drogodependientes desde el que mediante un enfoque biopiscosocial se aborda la deshabituación-desintoxicación de sujetos que sufren cualquier subtipo de patología adictiva (abuso/ dependencia).El equipo profesional de la UCA esta compuesto por un médico, un psicólogo y un ATS/ DUE. Las funciones que se realizan desde el área de enfermería difieren a las que se realizan en otros dispositivos asistenciales de índole sanitaria, debi...

    20. Comunicación social desde contextos de encierro

      López, Yemina

      2015-01-01

      “Comunicación Social desde los contextos de encierro. Desafíos y representaciones de acceder a una carrera de grado desde el penal” es una investigación de grado que desarrolé entre los años 2014 y 2015, con el fin de analizar las representaciones sociales que se dan a partir del Programa Educación Superior en Cárceles de la Facultad de Periodismo y Comunicación Social. En el devenir de este tiempo busqué recoger y analizar los discursos procedentes de los/as estudiantes que, privados/as de l...

    1. METABOLITOS AISLADOS DE Zanthoxylum rhoifolium

      Luis Enrique Cuca Suárez

      2008-04-01

      Full Text Available Una nueva cumarina llamada 3-metoxi-4-(3-metilbut-2-enil-2H-cromen-2-ona fue aislada de la madera de Z. rhoifolium, junto con los compuestos, conocidos dictamnina y N-metilflindersina. Sus estructuras fueron elucidadas por RMN, incluyendo técnicas bidimensionales y por comparación con datos reportados en la literatura.

    2. Evaluación de la actividad desulfurizadora de aislados nativos de Pseudomonas spp. en presencia de hidrocarburo Desulfurization activity evaluation of native strains of Pseudomonas spp. in the presence of hydrocarbon

      Alméciga-Díaz Carlos Javier

      2005-07-01

      Full Text Available El principal inconveniente en la combustión de los hidrocarburos es la conversión del azufre y el nitrógeno a sus respectivos óxidos, los cuales participan en la formación de lluvia acida y deterioran el medio ambiente e infraestructuras. La remoción de azufre a partir de compuestos órgano-azufrados mediante el uso de microorganismos ha surgido como una alternativa frente al proceso catalítico de hidrodesulfurización (HDS. En el presente trabajo se evaluó la actividad desulfurizadora de veintitrés aislados nativos de Pseudomonas spp. sobre dibenzotiofeno (DBT, usando un sistema de fermentación con igual proporción de fase acuosa y orgánica (n-hexano en presencia de oleato de etanolamina. Los aislados 02,05 y 06 conservaron su viabilidad en este medio y presentaron una remoción de azufre entre 6,0 y 9,4%, generando los metabolitos DBT-sulfona, DBT-sulfóxido, 2-hidroxibifenilo (2-HBP y sulfato presentes en la ruta metabólica 4S. Con estos aislados se evaluó la actividad desulfurizadora sobre keroseno y se observó una remoción de azufre entre 19,9 y 62,6% y una disminución del poder calorífico entre 0,45 y 5,55%. Palabras clave: dibenzotiofeno, desulfurización, Pseudomonas spp., keroseno.The main difficulty with fossil fuel combustión lies in sulphur and nitrogen becoming converted to their respective oxides, forming part of the acid rain which deteriorates the environment and infrastructure. Removing sulphur from organo-sulfur compounds by using micro-organisms has become an alternative to hydrodesulphurisation (HDS. Twenty-three Pseudomonas spp. native strains' desulphurisation activity on dibenzothiophene (DBT was evaluated by using a fermentation system having equal proportions of aqueous and organic (n-hexane phases in the presence of ethanolamine oléate. The 02, 05 and 06 strains maintained their viability in this médium, presenting 6,0% to 9,4% sulphur removal, producing DBT-sulphone, DBT-sulphoxide, 2

    3. Efecto de la temperatura sobre películas de un aislado proteico del suero bovino (WPI adsorbidas sobre la interfase aceite-agua

      Rodríguez Patino, Juan M.

      2002-09-01

      Full Text Available Heat-induced interfacial aggregation of a whey protein isolate (WPI with a high content of β-lactoglobulin, previously adsorbed at the oil-water interface, was studied by interfacial dynamic characteristics (interfacial tension and surface dilational properties performed in a automatic drop tensiometer coupled with microscopic observation and image analysis of the drop after heat-treatment. The temperature, ranging between 20 and 80 ºC, and protein concentration in aqueous bulk phase, ranging between 1.10-1 and 1.10-5% wt/wt, were studied as variables. The pH, and ionic strength were maintained constant at 5 and 0.05 M, respectively. During the heat-treatment, WPI films behave typically as viscoelastic with non-zero phase angle, but with increasing elastic characteristics as the heat-treatment progresses. During isothermal treatment the surface dilational modulus, E, increases and the interfacial tension, σ, and phase angle, φ, decrease with time to a plateau value. The time dependence of E can be quantified by a first-order equation according to two kinetic mechanisms. The rate of thermal changes in WPI adsorbed films increases with protein concentration in solution. Heat-treatment produces irreversible changes in WPI adsorbed films because the interfacial characteristics do not return to original values after cooling back to the initial temperature. Significant changes in interfacial characteristics and drop image associated with interfacial WPI gelation were observed at protein concentration as low as 1.10-5% wt/wt, even for heat-treatment at 40 ºC.En este trabajo se ha estudiado la agregación interfacial inducida por el calor en un aislado proteico del suero bovino (WPI, previamente adsorbido sobre la interfase aceite-agua. Se ha seguido la evolución, durante el tratamiento térmico, de las características dinámicas interfaciales (tensión interfacial y propiedades dilatacionales superficiales, determinadas en un tensi

    4. Caracterización molecular y agronómica de aislados de Trichoderma spp nativos del noreste de México

      José Luis Hernández Mendoza

      2012-06-01

      Full Text Available Normal 0 21 false false false ES-CO X-NONE X-NONE MicrosoftInternetExplorer4 Título en ingles: Molecular and agronomic characterization of Trichoderma spp natives of northeastern Mexico  Resumen Trichoderma sp es un hongo frecuentemente usado en actividades agrícolas, pues actúa como antagonista de diversas especies de hongos fitopatógenos. En este estudio se realizó el aislamiento de cuatro cepas de Trichoderma sp nativas del noreste de México, las cuales fueron identificadas a nivel molecular mediante la secuenciación del ITS 1. Además se evaluó su capacidad antagonista en contra los hongos fitopatógenos Macrophomina phaseolina y Fusarium oxysporum, que afectan severamente cultivos de sorgo, maíz y fríjol en el noreste de México. La identificación se realizó de acuerdo al grado de concordancia con secuencias reportadas y corresponden a las especies T. hammatum (HK701;  T. koningiopsis (HK702; T. asperellum (HK703 y Trichoderma sp (HK704. Por otra parte, las pruebas de antagonismo muestran que los aislados HK701, HK703 y HK704 inhiben por competencia el crecimiento de M. phaseolina y F. oxysporum, mientras que HK702 tiene la capacidad para hiperparasitar dichos fitopatógenos. Finalmente, se evaluó la promoción de crecimiento de T. asperellum HK703, en maíz (Pionner 30P49®, usando para ello concentraciones de tratamiento de 1x10e2 hasta 1x10e6 esp/mL. En estos ensayos se midió la producción de biomasa. Los resultados muestran que en concentraciones intermedias se tiene el mayor incremento en altura de plantas y mayor producción de peso seco en follaje y raíz. Entre los parámetros antes mencionados existen diferencias significativas. Palabras clave: control biológico, antagonismo, biofertilizantes, Zea maiz, Sorghum bicolor. Abstract Trichoderma sp is a fungus often used in agricultural activities, because it acts as an antagonist of several species of plant pathogenic fungi. In this study four strains of Trichoderma

    5. Liberación de endotelina-1 por angiotensina ll en miocitos cardíacos aislados Angiotensin II-induced endothelin-1 release in cardiac myocytes

      María C. Villa-Abrille

      2006-06-01

      Full Text Available Muchos de los efectos de la angiotensina II (Ang II son mediados en realidad por la acción de endotelina (ET endógena liberada y/o producida en respuesta a la Ang II. En este trabajo evaluamos la interacción Ang II/ET-1, sus consecuencias en la contractilidad cardíaca y el papel de las especies reactivas del oxígeno (EROs. Se usaron cardiomiocitos aislados de gato. La Ang II, 1 nM, produjo un efecto inotrópico positivo (EIP de 31.8±3.8% que fue cancelado por inhibición de los receptores AT1, de los receptores de ET, del intercambiador Na+/H+ (NHE, del modo inverso del intercambiador Na+/Ca2+ (NCX o por el secuestro de EROs. La Ang II, 100 nM, produjo un EIP de 70.5±7.6% que fue cancelado por inhibición de los receptores AT1 y bloqueado en parte por inhibición de los receptores de ET, del NHE, del modo inverso del NCX o por el secuestro de EROs. La Ang II, 1 nM, incrementó el ARNm de la preproET-1 lo cual fue anulado por el bloqueo de los receptores AT1. Los resultados permiten concluir que el EIP de la Ang II es debido a la acción de la ET-1 endógena liberada/formada por la Ang II. La ET-1 produce: estimulación del NHE, activación del modo inverso del NCX y un consecuente EIP. Dentro de esta cascada también participarían los EROs.Many of the effects thought to be due to angiotensin II (Ang II are due to the release/formation of endothelin (ET. We tested whether Ang II elicits its positive inotropic effect (PIE by the action of endogenous ET-1 and the role played by the reactive oxygen species (ROS in this mechanism. Experiments were performed in cat isolated ventricular myocytes in which sarcomere shortening (SS was measured to asses contractility after pharmacological interventions and the effect of Ang II on inotropism were analyzed. Ang II 1 nM increased SS by 31.8±3.8% (p<0.05. This PIE was cancelled by AT1 receptor blockade, by ET-1 receptors blockade, by Na+/H+ exchanger (NHE inhibition, by reverse mode Na+/Ca2

    6. Crisis vital desde una perspectiva de género

      Castro, Eva; Flores López, Olga

      2004-01-01

      A continuación se presenta el caso de una mujer de 55 años que presenta una crisis de identidad, abordado desde una perspectiva de género y un modelo explicativo y terapéutico que incluye elementos del análisis transaccional y la terapia constructivista.

    7. SPSS: Importar desde hoja de cálculo

      González Such, José

      2014-01-01

      S'ilustra la forma d'importar dades des de una fulla de càlcul a SPSS Se indica la forma de importar datos a SPSS desde una hoja de cálculo It is described the way for import data from an Excell Sheet

    8. Diversidad genética de cepas de Actinobacillus pleuropneumoniae (App aisladas desde planteles de producción intensiva de cerdos en Chile Genetic diversity of Actinobacillus pleuropneumoniae (App strains in intensive swine farms in Chile

      V Neira-Ramírez

      2012-01-01

      Full Text Available Actinobacillus pleuropneumoniae (App es el agente etiológico de la pleuroneumonía contagiosa porcina, una de las enfermedades de etiología bacteriana de mayor relevancia en producción porcina. En el mundo se han descrito 15 serotipos de App, en Chile solo los serotipos 1 y 5. La serotipificación requiere mucho tiempo, trabajo y dinero, actualmente se encuentran herramientas moleculares para realizar una "serotipificación" mediante la genotipificación de toxinas Apx. Así, se evaluaron 60 aislados de App provenientes de nueve empresas porcinas de producción intensiva distribuidas en distintas regiones de Chile, obtenidas desde pulmones de cerdos con lesiones compatibles con pleuroneumonía contagiosa porcina. Las bacterias fueron aisladas mediante los métodos tradicionales y confirmados por API, recolectados durante los años 2007, 2008 y 2009. Los resultados identificaron los genotipos correspondientes sólo a los serotipos 4, 6 y 7, los cuales se describen por primera vez en Chile, siendo el más frecuente el serotipo 7. En las diferentes zonas estudiadas, no existió un serotipo predominante, excepto en las regiones de O'Higgins y del Biobío en las cuales fue más frecuentemente aislado el serotipo 7. El presente estudio es el primer acercamiento con el fin de conocer la distribución de serotipos de App en Chile. Con el fin de conocer la real diversidad genética y serotipos de App en los diversos planteles en Chile es necesario realizar estudios que contemplen un mayor número de aislados.Actinobacillus pleuropneumoniae (App is the etiologic agent of porcine contagious pleuropneumonia, an important bacterial disease in intensive pig production. In the world were described 15 App serotypes, in Chile serotypes 1 and 5 have been reported. The serotyping technique is slow, expensive and difficult; currently, a molecular tool named PCR is available to "serotyping" by Apx toxins genotyping, which is quick, non-expensive and easy. 60 App

    9. ELABORACIÓN Y CARACTERIZACIÓN DE PELíCULAS DE GLICOPROTEÍNAS OBTENIDAS MEDIANTE REACCIÓN DE MAILLARD UTILIZANDO ALMIDÓN ACETILADO y AISLADO PROTEICO DE SUERO LÁCTEO

      P.B. Zamudio-Flores; L.A. Bello-Pérez

      2013-01-01

      Se realizó una acetilación del almidón de avena a dos diferentes niveles (bajo y medio), y se caracterizaron mediante análisis químico proximal, difracción de rayos X y microscopia electrónica de barrido. Los almidones acetilados se sometieron a una reacción de Maillard con aislado proteico de suero lácteo, obteniéndose glicoproteínas con diferente grado de glicosilación (alto, medio y bajo), las cuales se caracterizaron por el método espectrofotométrico del O-ftaldialdebído (OPA). Con las gl...

    10. Efectos de la substitución de conexina 43 por conexina 32 en la tolerancia a la isquemia y en la protección por precondicionamiento en corazones aislados de ratón

      Sánchez García, José Antonio; Rodríguez Sinovas, Antonio; García-Dorado García, Diego

      2011-01-01

      La conexina 43 es una proteína que juega un papel importante en la fisiopatología de la isquemia - reperfusión y en la señalización de la protección cardíaca. Para investigar si este papel está relacionado con las propiedades específicas de esta isoforma, se utilizó un modelo de corazón aislado de ratones transgénicos knock-in, en los que la conexina 43 se reemplazó por conexina 32. Los resultados de este trabajo demuestran un efecto modulador de la conexina 43 en el metabolismo energético, e...

    11. XML In Vitro Comparison of MIC Crude Extracts of Active Actinomycetes Isolated with Terbinafine, Griseofulvin Ketoconazole and Fluconazole against Microsporum Canis, Microsporum Gypseum and Trichophyton Mentagrophytes

      Keikha, N. (MSc; Ayatollahi Mousavi, SA. (PhD; Nakhaei, AR. (PhD; Amanloo, S. (MSc); Shah idi Benjar, GH. (PhD); Amiri , S . (MSc)

      2015-01-01

      Background and Objective: Dermatophytes are the fungi that have the ability to attack the keratinized tissues such as the skin, hair and nails. Infections caused by these organisms are named dermatophytosis. We aimed to compare Minimum inhibitory concentration (MIC) of Crude extracts of Active Actinomycete Isolates with Terbinafine, Griseofulvin, Ketoconazole and Fluconazole Drugs against Microsporum Canis, Microsporum gypseum and Trichophyton mentagrophytes. Material and Methods: In this exp...

    12. Degradation of Textile Dye Reactive Navy – Blue Rx (Reactive blue–59) by an Isolated Actinomycete Streptomyces krainskii SUK – 5

      Mane, U. V.; Gurav, P. N.; Deshmukh, A.M.; Govindwar, S. P.

      2008-01-01

      The isolated Actinomycete, Streptomyces krainskii, SUK -5 was found to decolorize and degrade textile dye Reactive blue–59.This azo dye was decolorized and degraded completely by Streptomyces krainskii SUK–5 at 24 h in shaking condition in the nutrient medium at pH 8. Induction in the activity of Lignin Peroxidase,and NADH-DCIP Reductase and MR reductase represents their role in degradation .The biodegradation was monitored by TLC, UV vis spectroscopy, FTIR. and GCMS analysis. Microbial and p...

    13. Cuba desde la posguerra fría: dilemas y desafíos

      Roberto González Arana

      2003-01-01

      Full Text Available Al cumplirse 45 años del triunfo de la revolución en Cuba resulta opor-tuno reflexionar sobre las vicisitudes de este país caribeño, aislado de losplanes del ALCA, marginado de la OEA y distante de la Unión Europea, ya quese observan evidentes contrastes entre la apremiante necesidad de una ma-yor integración de la Isla al mundo capitalista y la oleada de acciones drásti-cas de este régimen contra la oposición política interna.

    14. Huincúl Kimvn. Mirando la Historia "desde arriba".

      Pedro Canales Tapia

      2013-01-01

      Full Text Available El siguiente trabajo es una proposición histórica regional, de carácter etnográfica, que amparada en las técnicas y propuestas emanadas desde la historia oral y los estudios de la memoria, presentan una descripción de la situación vivencial de dos ancianos mapuche. Este relato muestra, a partir de la oralidad de los sujetos, la conexión, disociación y conflicto entre historia de vida e historia nacional - hegemónica, dando cuenta del sitial marginal y pauperizado, que la población mapuche experimenta de manera estructural, desde la pérdida de su territorio y la desarticulación de éste en el siglo XIX en adelante. De este modo, la historia mapuche se articula a partir de los dispositivos de la memoria y su vínculo con la alteridad, desde posiciones marcadas por la colonialidad de las relaciones sociales entre sí y con otros/as. De esta forma, rescatar la memoria histórica de dos  ancianos mapuche, nos adentra en la discusión acerca dela construcción constante de identidad étnica y despliegue de la etnicidad entre los miembros de un grupo determinado, emergiendo de allí discursos culturales, políticos y demandas sociales,que interpelan a la sociedad colonizadora y hegemónica, desde ámbitos tan diversos como laritualidad, el medio ambiente y la relación hombre – naturaleza.

    15. Cirugía de reemplazo valvular aórtico aislado en pacientes octogenarios: evaluación, riesgo operatorio y resultados a mediano plazo

      Fernando Piccinini

      2010-01-01

      Full Text Available RESUMENIntroducciónLas indicaciones y los beneficios del reemplazo valvular aórtico han sido claramente establecidos, aun para poblaciones añosas, de aumentada comorbilidad. Aun así, la manipulación de ateromas aórticos, el uso de circulación extracorpórea y de clampeo aórtico, el paro cardíaco y la descalcificación ligadas a esta técnica y sus eventuales consecuencias generan dudas acerca de su indicación en este grupo de pacientes.ObjetivosComunicar la morbimortalidad del procedimiento en octogenarios y validar la utilidad de los puntajes de predicción de riesgo utilizados más frecuentemente.Material y métodosSe estudiaron 87 pacientes octogenarios sometidos a reemplazo aórtico aislado; se analizaron variables preoperatorias, intraoperatorias y posoperatorias y se aplicó el Euroscore; para facilitar el subanálisis del Euroscore logístico, la población se dividió en tres grupos: riesgo bajo, moderado y alto. Se utilizó técnica quirúrgica convencional. Para el seguimiento, se analizó la historia clínica y/o se realizó encuesta telefónica.ResultadosLa mediana de edad fue de 83 ± 2,5 años, con un rango de 80 a 89 años. El 60% de la población en estudio era de sexo femenino. La indicación quirúrgica fue estenosis 92%, endocarditis activa 4,6% y enfermedad valvular 3,4%. Antecedentes: hipertensión 71%, tabaquismo 31%, dislipidemia 39%, diabetes 11,5%, EPOC 10%, IRC-diálisis 2,3%, ACV 11,5%, IAM previo 8%, fibrilación auricular 16%, reoperación 15%. Cuadro clínico: asintomático 3,45%, angina crónica estable 10,3%, angina inestable 11%, ICC 13,8%, disnea CF III-IV 60%. Disfunción ventricular izquierda moderada-grave 18,3%. Riesgo por Euroscore logístico 12,4% ± 15%. El tiempo operatorio promedio fue de 200 ± 61,7 min, el de CEC de 86 ± 32,5 min y el de clampeo aórtico de 65 ± 18,2 min. Complicaciones: sangrado médico 17,2%, reoperación sangrado 5,7%, bajo gasto cardíaco 13,8%, inotrópicos > 48 horas

    16. Vasodilator profile of flavonoid and phenylbutanoid compounds isolated from Croton schiedeanus Schlecht PERFIL VASODILATADOR DE COMPUESTOS FLAVONOIDES Y FENILBUTANOIDES AISLADOS DE CROTON SCHIEDEANUS SCHLECHT

      Sofía Ximena Correa-Hernández

      2008-12-01

      para el tratamiento de la hipertensión arterial. Objetivo. Evaluar el efecto vasodilatador de los flavonoides: 3-O-metilquercetina, 3,7-di-O-metilquercetina, y 3,3',4',7-tetra-O-metilquercetina; y los fenilbutanoides: (2S-7,9-dime--to--xiro---doden-drol, (2S-2-acetato de 7,9-dime-toxirododendrol y (2S- 2,8-diacetato de 7,9-dimetoxirodo-dendrol en anillos de aorta de ratas Wistar. Material y métodos. Estos compuestos se evaluaron en anillos de aorta precontraídos con fenilefrina (1 µM o KCl (80 mM. Para examinar posibles interacciones con endotelio, óxido nítrico, guanilato ciclasa, prostanoides o canales de K+ATP, aquéllos con mayores efectos vasodilatadores: 3-O-metilquercetina y 3,7-di-O-metilquercetina, se evaluaron en anillos estimulados con fenilefrina en presencia o ausencia de: endotelio, L-NAME (G -nitro-L-Arginina-Metil Ester, 100 µM, ODQ (1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-ona, 1 µM, meclofenamato sódico (10 µM o glibenclamida (1 µM. Resultados. En contraste con los compuestos fenilbutanoides que no arrojaron actividad relajante significativa, 3-O-metilquercetina y 3,7-di-O-metilquercetina mostraron una respuesta importante con concentraciones inhibitorias 50 (CI50 de 2,5 y 4,9 µM respectivamente frente a fenilefrina. ODQ y L-NAME desplazaron efectivamente a la derecha la curva dosis-respuesta, en particular la 3-O-metilquercetina (razón de IC50: 7,4 y 3,8. Conclusión. 3-O-metilquercetina y 3,7-di-O-metilquer-cetina, flavonoides aislados de Croton schiedeanus, ejercen importantes efectos vasodilatadores vinculados con la vía de NO/GMPc. Estos resultados soportan al uso etnobotánico de esta especie.

    17. A novel alkaloid from marine-derived actinomycete Streptomyces xinghaiensis with broad-spectrum antibacterial and cytotoxic activities.

      Wence Jiao

      Full Text Available Due to the increasing emergence of drug-resistant bacteria and tumor cell lines, novel antibiotics with antibacterial and cytotoxic activities are urgently needed. Marine actinobacteria are rich sources of novel antibiotics, and here we report the discovery of a novel alkaloid, xinghaiamine A, from a marine-derived actinomycete Streptomyces xinghaiensis NRRL B24674(T. Xinghaiamine A was purified from the fermentation broth, and its structure was elucidated based on extensive spectroscopic analysis, including 1D and 2D NMR spectrum as well as mass spectrometry. Xinghaiamine A was identified to be a novel alkaloid with highly symmetric structure on the basis of sulfoxide functional group, and sulfoxide containing compound has so far never been reported in microorganisms. Biological assays revealed that xinghaiamine A exhibited broad-spectrum antibacterial activities to both Gram-negative persistent hospital pathogens (e.g. Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli and Gram-positive ones, which include Staphylococcus aureus and Bacillus subtilis. In addition, xinghaiamine A also exhibited potent cytotoxic activity to human cancer cell lines of MCF-7 and U-937 with the IC50 of 0.6 and 0.5 µM, respectively.

    18. Genetic transformation of marine Actinomycete sp. Isolate M048 and expression of a recombinant plasmid carrying the apc gene

      HOU Yanhua; LI Fuchao; QIN Song; WANG Quanfu

      2006-01-01

      Optimal conditions for protoplasts formation of marine Actinomycete sp. isolate M048 were described, dense and disperse mycelia were cultured in SGGP medium, 0.5% glycine, lysozyme exposure (2 mg/cm3, 37 ℃, 40 min), and the concentration of sucrose in protoplast buffer was 0.4 mol/dm3 for keeping the balance of osmotic pressure. Using PEG-mediated protoplasts transformation, the transformation frequency was 89 transformants per microgramme of pIJ702. Meanwhile, an effective transformation procedure was established based on intergeneric conjugation from E. coli ET12567 (pUZ8002) using shuttle vectors pPM801, pPM803 and a(ψ)C31-derived integration vector pIJ8600 containing oriT and attP fragments. Transformation frequencies were 5.30×10-4±0.26×10-4, 8.92×10-4±0.19×10-4 and 6.38×10-5±0.41×10-5, respectively. Further, the heterologous expression of the allophycocyanin gene (apc) in the strain M048 was used to demonstrate this transformation system. SDS-PAGE and Western blot analysis confirmed the expression of recombinant APC (rAPC).

    19. Evaluación in vitro de la actividad inhibitoria de extractos vegetales sobre aislados de Colletotrichum spp In vitro evaluation of inhibitory activity of plant extracts on Colletotrichum spp

      Alexander Pérez Cordero

      2011-04-01

      Full Text Available Se evaluó la actividad inhibitoria in vitro de extractos de hojas de Melissa officinalis, Origanum vulgare, Jatropha gossypilia, Eucalyptus sp., Melia azederach y Mascagnia concinna sobre aislados de hongo del género Colletotrichum, causante de la enfermedad antracnosis en el cultivo del ñame (Dioscorea alata, D. rotundata en el departamento de Sucre, Colombia. Para la obtención de extractos etanólicos de hojas de M. officinalis y O. vulgare, se utilizó el método de percolación y para las otras especies vegetales, el método de Soxhlet. Una vez preparados los extractos de hojas, se evaluó su actividad inhibitoria sobre seis aislados de hongo del género Colletotrichum (BVC 279a, BVC 279b, BVC 853, BVC 040, BVC 342 y BVC 507. Para la prueba inhibitoria se utilizó el método de siembra directa sobre la superficie del medio papa-dextrosa-agar. Sobre las diferentes cepas se adicionaron 250 µl de cada extracto por separado. Se utilizó un control positivo con nistatina (4 mg/ml y un testigo absoluto sin ningún tipo de tratamiento. La prueba se evaluó midiendo el crecimiento radial de cada cepa con los diferentes tratamientos a 96 y 168 h. Se utilizó un diseño de bloques completamente al azar con tres repeticiones, para probar diferencias significativas entre tratamientos, cepas (aislados y tiempo de inhibición. Los resultados expresados en porcentaje de inhibición, mostraron una alta actividad de los extractos obtenidos de las hojas de las plantas de M. azederach y M. concinna, sobre los hongos evaluados y su acción fue similar al control positivo.The objective of this study was to evaluate in vitro inhibitory activity of extracts from leaves of Melissa officinalis, Origanum vulgare, Jatropha gossypilia, Eucalyptus sp. Melia azederach and Mascagnia concinna on isolated fungus of the genus Colletotrichum, which causes anthracnose disease in the crop of yam (Dioscorea alata, D. rotundata in the department of Sucre, Colombia. To obtain

    20. El barroco visto desde la historia intelectual japonesa

      Falero Folgoso, Alfonso

      2004-01-01

      [ES] El término "barroco" en la historia cultural e intelectual japonesa es importado desde la lexicología europea. Por tanto más allá de posibles fenómenos estéticos paralelos que puedan haberse dado en los siglos XVII-XVIII en Japón, y en particular la historia del teatro kabuki o de la arquitectura de Nikko, este ensayo hace una revisión semiótica del uso del término "barroco" en los discursos que configuran la intelectualidad japonesa del siglo XX, y explora la heterogeneidad de su incide...

    1. Desde los bordes, hacia una natación regional

      Tévez, Nora Beatriz

      2011-01-01

      Los CEF de nuestra provincia constituyen instituciones educativas que construyen con la comunidad una Educación Física que atiende a la corporeidad y motricidad de los sujetos. El proyecto Desde los Bordes hacia una Natación Regional instituye una propuesta hacia lo político singular. Singular no como particular, sino como modo de hacer situacionales. Considera la diversidad cultural, la heterogeneidad de posibilidades, intereses y deseos que respondan a las múltiples infancias y adolescencia...

    2. EL CAPITAL INTELECTUAL CONSOLIDADO DESDE EL MODELO DE LIDERAZGO AMCO

      SANDRA ESTRADA MEJÍA; LUZ STELLA RESTREPO DE OCAMPO; MARÍA ESPERANZA LÓPEZ DUQUE

      2009-01-01

      Este artículo presenta una propuesta para fortalecer el capital intelectual desde la aplicación del modelo de liderazgo AMCO estructurado en 3 niveles: individual, social y de gestión, desarrollada a través de estrategias de intervención al capital humano, estructural y cliente. Es así como se logrará que la organización basada en el conocimiento oriente sus procesos a la generación de ciencia y tecnología para impactar las necesidades que hoy en día requiere la sociedad.

    3. Capital de Marca desde la perspectiva del consumidor

      José Luis Saavedra Torres

      2004-01-01

      El objetivo de este artículo es explorar los conceptos teóricos del Capital de Marca desde la perspectiva del comportamiento del consumidor, como un intento de revisar los basamentos que constituyen su formación. Los planteamientos realizados forman parte de una investigación cualitativa de las propuestas de cuatro autores que formulan modelos para definir las fuentes, métodos para construir y elementos para determinar los beneficios del Capital de Marca, tanto para la organizació...

    4. Las necesidades humanas desde la psicología moral

      Martí Vilar, Manuel

      2008-01-01

      En este texto se presentan las aportacionesde la psicología del desarrollo moral y de la prosocialidad a la reflexión sobre las necesidades humanas, especialmente en el contexto presente, haciendo especial hincapié en propuestas psicoeducativas con una solución positiva a la situación actual. La delimitación conceptual que se plantea pretende ayudar a entender aquellos términos que más se utilizan y que se consideran una buena aportación desde la psicología moral a las necesidades humanas. AB...

    5. Lo escrito desde el análisis del discurso

      Daniel Cassany

      2013-01-01

      El objetivo de este artículo es sintetizar las principales aportacionesque se han realizado desde el ámbito lingüístico (pragmática,análisis del discurso, lingüística del texto, sociolingüística; ver Lavandera1985) y etnográfico {estudio de comunidades orales y escritas;ver Saville-Troike 1989), para orientar tanto la tarea investigadoradel linguista y del psicopedagogo de la lectoescritura como laactividad didáctica del docente en el aula.

    6. Lo escrito desde el análisis del discurso

      Daniel Cassany

      2013-09-01

      Full Text Available El objetivo de este artículo es sintetizar las principales aportacionesque se han realizado desde el ámbito lingüístico (pragmática,análisis del discurso, lingüística del texto, sociolingüística; ver Lavandera1985 y etnográfico {estudio de comunidades orales y escritas;ver Saville-Troike 1989, para orientar tanto la tarea investigadoradel linguista y del psicopedagogo de la lectoescritura como laactividad didáctica del docente en el aula.

    7. Concretar respuestas desde el trabajo comunitario-1. parte

      Barbero, Josep Manel; Jornada INMODELS-UV: Nuevos desafíos sociales, nuevos modelos de intervención. Valencia, Abril 2011

      2011-01-01

      Aproximación al Trabajo Social desde una perspectiva metodológica, cuyo objetivo es la transformación de situaciones sociales, mediante una estrategia de organización colectiva. El Trabajo Comunitario se enfrenta al reto de constituir y sostener grupos motor, elaborar diagnósticos colectivos, y de proyectos de desarrollo social (generar nuevos grupos y nuevas relaciones entre grupos, intensificar la vida social), y la evaluación de su aplicación, con objetivos de proceso y objetivos de habil...

    8. PASIÓN, MEMORIA Y FANTASMAS DESDE EL PATIO 29

      Claudio Andrés Maldonado Rivera

      2008-01-01

      La memoria es uno de los mecanismos de reconstrucción cultural que posibilita entender la gestación de la identidad colectiva. En esta investigación se intenta dar cuenta, desde una perspectiva semiótica, de cómo la memoria activa la (re)construcción y mantenimiento identitario de la colectividad de la izquierda chilena dentro de un lugar codificado ideológicamente, la Necrópolis, en específico el Cementerio General de Santiago (C.G.S), en particular el patio 29, el cual acoge los cuerpos de ...

    9. La salud mental desde la transdisciplinariedad y el modelo integral

      José Domingo Flórez Moreno; Heimi Darlyn Gallego Jacded

      2011-01-01

      Se trata sobre la transdisciplinariedad, con el objetivo de analizar la temática y tener más elementos para el abordaje de la salud mental desde la integralidad en la psicología, teniendo como base el modelo integral de Ken Wilber. Para tal fin se realizó la búsqueda de información con base en seis ejes temáticos: salud mental, transdisciplinariedad, diferentes áreas de la salud mental y sus contribuciones, el modelo integral, nuevos paradigmas y la psicología integral. Se revisaron 89 artícu...

    10. La Conservación Integral Alternativa desde el Sur

      Torrealba, Isa; Carbonell, Fabricio

      2014-01-01

      Esbozamos los elementos de un nuevo modelo conceptual para entender desde la perspectiva latinoamericana qué ocasiona el desbalance entre naturaleza y sociedad y vislumbrar así las verdaderas oportunidades y amenazas de soslayar el abismo entre lo ambiental y lo social al integrar efectivamente desarrollo y conservación.  Como propuesta teórica endógena compatible con la perdurabilidad del bienestar humano y producto de 10 años de investigación en el medio rural latinoamericano (sur), present...

    11. El alcoholismo desde la perspectiva de género

      Janette Góngora Soberanes; Marco Antonio Leyva Piña

      2005-01-01

      Es evidente que ser mujer y presentar un consumo problemático de alcohol reta a los estereotipos sociales y culturales de una sociedad sexista. Se cree que, en relación con los hombres, las mujeres con problemas de alcohol tienen la tendencia a experimentar un estigma doloroso que destruye la imagen que tienen de ellas ante los demás, a diferencia de ellos. Así, además del estigma asociado a ser mujer enfer-ma para toda su vida desde su espacio privado, es mal juzgada en el ejercicio de surol...

    12. Caracterización molecular de la región determinante de resistencia a quinolonas (QRDR de la topoisomerasa IV de Bartonella bacilliformis en aislados clínicos

      Abraham Espinoza-Culupú

      2014-05-01

      Full Text Available Bartonella bacilliformis es el agente etiológico de la Enfermedad de Carrión, endémica del Perú. Pocas investigaciones han sido realizadas acerca de los genes asociados a la resistencia antimicrobiana en aislados clínicos de este patógeno. Estos genes no están caracterizados molecularmente, ni se conoce la región asociada a dicha resistencia. Por ello, el objetivo del este trabajo fue caracterizar molecularmente la región determinante de la resistencia a las quinolonas (QRDR en la topoisomerasa IV, que está codificada por los genes parC y parE, así como también desarrollar una prueba de susceptibilidad antimicrobiana para B. bacilliformis. Las muestras sanguíneas de 65 pacientes procedentes de La Libertad, Cusco, Ancash y Piura, se sembraron en placas de agar sangre e incubaron a 30 °C con 5% CO2. Luego se procedió a: (1 determinar la susceptibilidad antimicrobiana y (2 extraer el DNA genómico, amplificar los genes mencionados, secuenciarlos y analizarlos mediante herramientas bioinformáticas. Se obtuvieron 6 cultivos positivos. Los aislados fueron sensibles a la ciprofloxacina (excepto uno procedente de Quillabamba-Cusco, que presentó susceptibilidad disminuida y resistentes al ácido nalidíxico. Del análisis de las secuencias aminoacídicas de ParC y ParE de B. bacilliformis se concluye que presentan diferencias aminoacídicas en comparación con las secuencias de las proteínas respectivas de E. coli K12 MG1655, que probablemente confieran resistencia al ácido nalidíxico pero no a la ciprofloxacina. Se determinó que las QRDR de las proteínas ParC y ParE de B. bacilliformis están comprendidas entre los aminoácidos 67 al 118 y 473 al 530, respectivamente. El antibiograma y la concentración mínima inhibitoria se evalúan mejor usando inóculos a escala 1 de McFarland y a los 6 días de incubación.

    13. Genotipos de aislados de campo de Brucella abortus de distintas regiones geográficas de Chile Genotypes of Brucella abortus field isolates from different geographical regions of Chile

      M Mancilla

      2008-01-01

      Full Text Available La brucelosis bovina es una enfermedad zoonótica, endémica de alto impacto económico. La identificación genética de las cepas prevalentes de Brucella abortus, el patógeno, es clave para establecer estrategias epidemiológicas de control de la enfermedad. La secuencia de inserción IS711 ha sido utilizada como un marcador genético para diferenciar entre especies de Brucella, miembros de una misma especie y dentro de un mismo biovar. Hemos analizado los perfiles de IS711-RFLP de 46 aislados de B. abortus, recolectados durante el periodo 1997-2005, provenientes de 16 áreas geográficas diferentes de Chile. Todos los aislados fueron previamente identificados como B. abortus biovar 1, utilizando las técnicas convencionales. De estos, el 87% compartieron el mismo perfil de IS711-RFLP, mientras que el 8,7% correspondió al patrón de la cepa vacuna RB51. En este trabajo se informa el hallazgo de dos cepas indistinguibles por PCR AMOS con perfiles nuevos de IS711-RFLP, no reportados previamente.Bovine brucellosis is an endemic, zoonotic disease of high economic impact. The genetic identification of the prevalent Brucella abortus strains, the pathogen, is key to pursue further epidemiological strategies for disease control. The insertion sequence IS711 has been used as genetic marker to differentiate among Brucella species, members of the same specie and within the same biovar. We have analyzed the IS711-RFLP pattern for 46 B. abortus isolates, collected during the period of 1997-2005 from 16 different geographical areas of Chile. All isolates were previously identified by conventional techniques as B. abortus biovar 1. Of these, 87% sharedthesame IS711 DNA profile, while an 8.7 % corresponded to the pattern of RB51 vaccine strain. We report the finding of two new strains, not differentiated by AMOS PCR, which showed unreported patterns of IS711-RFLP.

    14. Biological Control of Sclerotinia sclerotiorum, Causal Agent of Sunflower Head and Stem Rot Disease, by Use of Soil borne Actinomycetes Isolates

      F. Baniasadi

      2009-01-01

      Full Text Available Problem statement: High level of biosafety and non adverse effects on the environment of biocontrol strategies of pest management, are priorities of tomorrow's world agriculture. Actinomycetes are active biocontrol agents due to their antagonistic properties against wide range of plant pathogens particularly fungi. Fungal pathogens are liable for a big part of damages in agriculture economy. Approach: In the present research antifungal bioactivity of 50 isolates of Actinomycetes collected from soils of Kerman province of Iran was investigated against Sclerotinia sclerotiorum (Lib., the causal agent of stem rot in sunflower, through agar disc method and dual culture bioassays. The Streptomyces isolate No. 363 was propagated in submerged cultures and active crude was prepared upon which several biological characterizations performed. Greenhouse studies were achieved to confirm laboratory results. Results: Among the tested Streptomyces isolates, 10 isolates revealed antagonistic properties in dual culture procedure from which isolate No. 363 showed highest bioactivity. The active metabolite of Streptomyces isolate No. 363 was polar and well soluble in H2O. Using agar-disc method, progressive growth of the pathogen was highly reduced by the antagonist through exhibiting ability to constitute fungus-free zones of inhibitions. The results indicated that isolate No. 363 was a proper candidate for field biocontrol studies. Conclusion: Results may open a horizon for production of resistant transgenic plants having antifungal properties originated from biologically active Streptomyces spp. recognition and production of effective metabolite(s of Streptomyces spp. which was responsible for antifungal activities will be our commercial goal due to rich reserves of soil borne Actinomycetes in Iran.

    15. Utilization of Agro-industrial Wastes for the Simultaneous Production of Amylase and Xylanase by Thermophilic Actinomycetes

      Renu Singh

      2012-12-01

      Full Text Available Agro-industrial wastes such as sugarcane bagasse, wheat bran, rice bran, corn cob and wheat straw are cheapest and abundantly available natural carbon sources. The present study was aimed to production of amylase and xylanase simultaneously using agro-industrial waste as the sole carbon source. Seven thermophilic strains of actinomycete were isolated from the mushroom compost. Among of these, strain designated MSC702 having high potential to utilize agro-industrial wastes for the production of amylase and xylanase. Strain MSC702 was identified as novel species of Streptomyces through morphological characterization and 16S rRNA gene sequence. Enzyme production was determined using 1% (w/v of various agro-industrial waste in production medium containing (g/100mL: K2HPO4(0.1, (NH42SO4(0.1, NaCl (0.1, MgSO4(0.1 at pH 7.0 after incubation of 48 h at 50°C. The amylase activity (373.89 IU/mL and xylanase activity (30.15 IU/mL was maximum in rice bran. The decreasing order of amylase and xylanase activity in different type of agro-industrial wastes were found rice bran (RB > corn cob (CC > wheat bran (WB > wheat straw (WS > sugarcane bagasse (SB and rice bran (RB > wheat bran (WB > wheat straw (WS > sugarcane bagasse (SB > corn cob (CC, respectively. Mixed effect of different agro-industrial wastes was examined in different ratios. Enzyme yield of amylase and xylanase was ~1.3 and ~2.0 fold higher with RB: WB in 1:2 ratio.

    16. Optimizing water treatment practices for the removal of actinomycetes and earthy odor in water of Bovilla reservoir

      ADELA KULLAJ

      2014-06-01

      Full Text Available Bovilla reservoir, which is situated 15 km North-East of Tirana the capital city of Albania is one of the major hidrotechnical works of this country. This reservoir is a warm monomictic water body and stratifies higher in the summer season. The predominant trophic state of Bovilla reservoir is oligotrophy. From autumn 2001 this reservoir repeatedly manifests an unpleasant taste and odor which is defined as musty- earthy. Taste and odor control has become an important issue for drinking water suppliers worldwide. Consumers react very sensitively to changes in the organoleptic quality of their drinking water. The reason is that odor compounds present a very low threshold of perception (10–20 ng/L. Bovilla water treatment plant treats 1800 L/s raw water taken from Bovilla reservoir, using oxidation, coagulation and flocculation, sedimentation, filtration and disinfection process. In cases of bad odor powdered activated carbon (PAC is added at the rapid mix section. Throughout the monitoring period were done: quality and sensory analysis of raw water on a weekly frequency, analysis of treated water after coagulation, laboratory scale experiments using different doses of chemicals, applying optimized doses in full scale and PAC adsorption experiments. The aims of this study were: to predict the PAC doses required to treat water of Bovilla reservoir containing bad taste and odor, to establish the removal efficiency of taste and odor by three types of activated carbons with different iodine number and to assess the impact of NaOCl and other chemical in the treatment process of the plant in removing actinomycetes and bad odor. Results have shown that traditional treatment processes are usually inadequate in removing taste and odor and optimization of plant practices is required. Powdered activated carbon (PAC can effectively remove taste and odor when the correct dose is applied.

    17. Micromonospora taraxaci sp. nov., a novel endophytic actinomycete isolated from dandelion root (Taraxacum mongolicum Hand.-Mazz.).

      Zhao, Junwei; Guo, Lifeng; He, Hairong; Liu, Chongxi; Zhang, Yuejing; Li, Chuang; Wang, Xiangjing; Xiang, Wensheng

      2014-10-01

      A novel actinomycete, designated strain NEAU-P5(T), was isolated from dandelion root (Taraxacum mongolicum Hand.-Mazz.). Strain NEAU-P5(T) showed closest 16S rRNA gene sequence similarity to Micromonospora chokoriensis 2-19/6(T) (99.5%), and phylogenetically clustered with Micromonospora violae NEAU-zh8(T) (99.3%), M. saelicesensis Lupac 09(T) (99.0%), M. lupini Lupac 14N(T) (98.8%), M. zeae NEAU-gq9(T) (98.4%), M. jinlongensis NEAU-GRX11(T) (98.3%) and M. zamorensis CR38(T) (97.9%). Phylogenetic analysis based on the gyrB gene sequence also indicated that the isolate clustered with the above type strains except M. violae NEAU-zh8(T). The cell-wall peptidoglycan consisted of meso-diaminopimelic acid and glycine. The major menaquinones were MK-9(H8), MK-9(H6) and MK-10(H2). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were C(16:0), iso-C(15:0) and C(17:0). Furthermore, some physiological and biochemical properties and low DNA-DNA relatedness values enabled the strain to be differentiated from members of closely related species. Therefore, it is proposed that strain NEAU-P5(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora taraxaci sp. nov. is proposed. The type strain is NEAU-P5(T) (=CGMCC 4.7098(T) = DSM 45885(T)). PMID:25082023

    18. Degradation of Textile Dye Reactive Navy – Blue Rx (Reactive blue–59 by an Isolated Actinomycete Streptomyces krainskii SUK – 5

      Mane, U. V.

      2008-01-01

      Full Text Available The isolated Actinomycete, Streptomyces krainskii, SUK -5 was found to decolorize and degrade textile dye Reactive blue–59.This azo dye was decolorized and degraded completely by Streptomyces krainskii SUK–5 at 24 h in shaking condition in the nutrient medium at pH 8. Induction in the activity of Lignin Peroxidase,and NADH-DCIP Reductase and MR reductase represents their role in degradation .The biodegradation was monitored by TLC, UV vis spectroscopy, FTIR. and GCMS analysis. Microbial and phytotoxicity studies of the product were carried out.

    19. EL LENGUAJE DESDE LA BIOLOGÍA DEL AMOR

      JOSÉ DE LA FUENTE

      1997-01-01

      Full Text Available En el presente trabajo se intenta una lectura descriptiva, sistemática y analítica del aporte del biólogo Humberto Maturana sobre su invitación teórica a la biología del conocimiento y además correlatos conceptuales remitidos al lenguaje desde la biología del amor. Su planteamiento "conspirativo" es ontológico, fundado en la conversación y en la democracia. Cuando se pierde la congruencia entre nosotros y el medio, simplemente no somos. En esta teoría aparecen íntimamente ligadas las nociones de lenguaje, lenguajear, conversar, emocionar, observar y autopoiesis. Se reseña a la persona y al investigador y se esbozan controversias, se precisa qué es la biología del amor, el lenguaje, los sistemas de convivencia y dominios, la relación del lenguaje con la realidad, la observación desde los caminos explicativos de la objetividad sin paréntesis y de la objetividad en paréntesis, la importancia de la experiencia estética y se sugiere qué mundo nos espera a partir de la educación

    20. La prostitución, una mirada desde sus actores

      Luisa Fernanda Montoya Restrepo

      2015-01-01

      Full Text Available Este artículo surge de la investigación Aspectos sociales y familiares que conllevan al ejercicio de la prostitución a 14 jóvenes de la ciudad de Medellín, a partir de sus relatos de vida, que en su proceso de generación de información implementó entre otras estrategias, una serie de talleres con población –hombres y mujeres– en ejercicio de la prostitución y con el equipo profesional que les brinda atención psicosocial, como parte del desarrollo del diseño metodológico planteado para la generación de información. Con esta publicación se pretende reflexionar sobre las diferentes concepciones y significados que se le ha dado a la palabra prostitución, estigmatizada no solo desde el imaginario social, sino también desde la religión. Se retoman para ello algunas posturas disciplinares y especialmente las voces de los actores que participaron como fuentes originales en el proceso de investigación; por ende, se realizará un breve recorrido que recoja las connotaciones más comunes de las personas que ejercen cotidianamente la prostitución.

    1. Estudios hematológicos y patológicos comparativos de cerdos inoculados con un aislado de campo y el serotipo 5 ATCC de Actinobacillus pleuropneumoniae Comparative hematological and pathological study of inoculated pigs with a field isolate and an ATCC serotype 5 of Actinobacillus pleuropneumoniae

      D Muñoz

      2010-01-01

      Full Text Available Se realizó una inoculación experimental de A. pleuropneumoniae utilizando un aislado de campo y una cepa de referencia ATCC serotipo 5, para lo cual se utilizaron tres grupos de animales (n = 15 para cada grupo. El grupo 1 (G1 fue inoculado con medio estéril, el grupo (G2 con serotipo 5 ATCC y el grupo 3 (G3 fue inoculado con un aislado de campo (418/07. Los resultados mostraron diferencias significativas (P ≤ 0,05 en el recuento de leucocitos totales entre el grupo G1 v/s G2 y G1 v/s G3 y los grados de las lesiones pulmonares totales evidenciaron diferencias estadísticamente significativas (P ≤ 0,05 entre los tres grupos de estudio. Las lesiones histopatológicas pulmonares mostraron diferencias estadísticas relevantes sólo entre G1 y G3 (P ≤ 0,05. En este trabajo se verifican diferencias importantes del comportamiento entre el aislado de campo y el serotipo 5 ATCC, sobre los cambios hematológicos y las lesiones macroscópicas e histopatológicas ocasionadas por ellos, lo cual podría indicar una mayor virulencia y patogenicidad del aislado nacional. Se espera en un futuro próximo serotipificar este aislado nacional de App.An experimental inoculation of Actinobacillus pleuropneumoniae (App was carried out with a field isolate and an ATCC serotype 5. Three groups of 15 pigs each were used. Group 1 (G1 was the control group inoculated with sterile media, Group 2 was inoculated with the serotype 5 ATCC, and Group 3 (G3 was inoculated with a field isolate (418/07. The results showed statistically significant differences (P ≤ 0.05 in the total leukocytes count between G1 v/s G2 and G1 v/s G3. The total macroscopic lung lesions scores were statistically different among the 3 groups (P ≤ 0.05. However, statistical difference was found only between G1 and G3 in the histopathological lung lesions (P ≤ 0.05. This work shows a clear difference in the hematological changes and the macroscopic and histopathological lesions between the

    2. 嗜盐嗜碱放线菌的研究进展与展望%Research on Progress of Halophilic Basophilic Actinomycetes

      加苏尔·阿不都克里木; 旭格拉; 塔衣尔; 阿不都克里木·热依木; 木尼热木·阿力木江; 迪丽拜尔·托合提

      2015-01-01

      Haloalkaliphilic extremophiles were survied in saline alkali soil. Halophilic basophilic Actinomycetes with the genetic characteristics and the special supersedes way, produced a variety of secondary me⁃tabolites, was one of microbial resources with great application prospect. This paper mainly reviewed Halophilic ba⁃sophilic Actinomycetes Resources, standard classification, distribution, system science, development and utilization prospects.%嗜盐嗜碱极端微生物主要分布于地球上的盐碱土壤中,嗜盐嗜碱放线菌适应并长期生存这一特殊极端环境,已具备独特的遗传特性和新陈代谢途径,能够产生具有开发利用价值的多种次生代谢产物,是一类极具应用前景的微生物资源。文章主要综述了嗜盐嗜碱放线菌的资源、分类标准、分布、系统学及其发展趋势、开发利用前景。

    3. Determination of the Residual Anthracene Concentration in Cultures of Haloalkalitolerant Actinomycetes by Excitation Fluorescence, Emission Fluorescence, and Synchronous Fluorescence: Comparative Study

      Reyna del Carmen Lara-Severino

      2016-01-01

      Full Text Available Polycyclic aromatic hydrocarbons (PAHs are compounds that can be quantified by fluorescence due to their high quantum yield. Haloalkalitolerant bacteria tolerate wide concentration ranges of NaCl and pH. They are potentially useful in the PAHs bioremediation of saline environments. However, it is known that salinity of the sample affects fluorescence signal regardless of the method. The objective of this work was to carry out a comparative study based on the sensitivity, linearity, and detection limits of the excitation, emission, and synchronous fluorescence methods, during the quantification of the residual anthracene concentration from the following haloalkalitolerant actinomycetes cultures Kocuria rosea, Kocuria palustris, Microbacterium testaceum, and 4 strains of Nocardia farcinica, in order to establish the proper fluorescence method to study the PAHs biodegrading capacity of haloalkalitolerant actinobacteria. The study demonstrated statistical differences among the strains and among the fluorescence methods regarding the anthracene residual concentration. The results showed that excitation and emission fluorescence methods performed very similarly but sensitivity in excitation fluorescence is slightly higher. Synchronous fluorescence using Δλ=150 nm is not the most convenient method. Therefore we propose the excitation fluorescence as the fluorescence method to be used in the study of the PAHs biodegrading capacity of haloalkalitolerant actinomycetes.

    4. Desarrollo sostenible: enfoques desde las ciencias económicas

      Carlos Andrés Vergara Tamayo

      2016-07-01

      Full Text Available El concepto de desarrollo sostenible ha sido objeto de gran discusión y controversia durante décadas, de cuestionamientos y debates teórico–prácticos en el ámbito disciplinar económico. Las teorías que soportan esta diversidad conceptual permiten identificar en el tiempo no solo la construcción del concepto, sino también la transformación que este sufre, ya sea por parte de enfoques disciplinares o también multidisciplinares, como una diversidad de posturas frente a la evolución del mismo. A partir de allí surge la necesidad de relacionar algunos elementos clave, así como los diferentes puntos del desarrollo sostenible, dadas las condiciones que dieron origen a este desde la economía como ciencia social.

    5. Algunas reflexiones desde la acuarela de Mario Conde

      Pamela Romano

      2012-11-01

      Full Text Available This brief reflection on the work of Mario Conde starts paying attention to the special relationship the artist has with the watercolor technique, deploying from that relationship a copy-based ethics. From that materiality Conde builds his response to the postmodern moment in which art in general, and particularly Bolivian art, is been developed given its current social conditions. The ethics of the copy is explored especially in two of his works: "Exterminator Angel" where  copying addresses colonial issues that return to question and challenge the very notion of homeland, and "Drunk with power (Géricault in Los Andes" where the copy,  adapted to Andean latitudes, exudes a desire to restore the sacred and  auratic character of artwork as well as of the Andean imagery.Esta breve reflexión sobre la obra de Mario Conde se inicia poniendo atención en la relación particular que tiene el pintor con la técnica de la acuarela, desplegando desde esa relación una ética basada en la copia. Desde esa materialidad Conde concretiza su respuesta al momento posmoderno en que se desarrolla el arte en general, y el boliviano en particular dadas sus condiciones sociales actuales. La ética de la copia es explorada especialmente en dos de sus obras: “Ángel exterminador” donde la copia aborda temáticas coloniales que retornan para poner en cuestión y entredicho la noción de patria, y “Ebrios de poder (Géricault en Los Andes” donde la copia adaptada a latitudes andinas destila un deseo de restablecer el carácter sagrado y aurático de la obra de arte como del imaginario andino.

    6. La Silvicultura: Desde sus Origenes hasta el Siglo XIX

      Del Valle A Jorge Ignacio

      1997-06-01

      Full Text Available Para este ensayo se empleó información secundaria publicado en revistas y libros que documentan el origen de la actividad silvícuola, coincidiendo y aun antecediendo al desarrollo de la agricultura. Luego se centra en la transformación de esta actividad en una ciencia, la silvicultura, o más propiamente, la dasonomía, la cual se consolidó desde mediados del siglo XVIII cuando se empezó a enseñar formalmente en Alemania y otros países germánicos y se crearon, además, servicios forestales como el de Prusia. De esta época datan los primeros conceptos teóricos del rendimiento sostenido de los bosques (G. L. Harting, H. von Cotta, aunque desde comienzos del siglo XIII ya existían bosques empíricamente ordenados con esta filosofía en el Centro de Europa. La sustentabilidad o sostenibilidad tan en boga hoy en la ecología y la economía, tiene allí su antecedente más remoto. También Francia jugó un papel preponderante en el desarrollo de la dasonomía, sobre todo por haber involucrado el manejo de las cuencas hidrográficas dentro de las ciencias forestales, en tanto que los aportes de los germanos fueron en economía y medición forestal. El origen de la profesión forestal parece situarse tanto en los guardabosques ingleses como en los maestros de aguas y bosques franceses, de principios de los siglos XII y XIII, respectivamente. Durante el periodo analizado las ciencias forestales evidenciaron relaciones estrechas con las ciencias agropecuarias con las cuales parece haberse asociado sólo durante el presente siglo por razones coyunturales. El siglo XIX vio nacer la silvicultura tropical en la India; los primeros profesores e investigadores fueron alemanes contratados por la corona inglesa. También en América fueron forestales alemanes quienes fundaron en Estados Unidos de América la primera escuela forestal y contribuyeron a crear el Servicio Forestal de dicho país.

    7. Homofobia: apreciaciones desde tres perspectivas psicológicas

      José Alonso Andrade-Salazar

      2016-09-01

      Full Text Available La presente investigación busca aproximarse a las causas psicosociales de la homofobia a través de una investigación cualitativa, con base al paradigma comprensivo-hermenéutico abordada desde los principios epistemológicos de tres escuelas psicológicas: psicoanálisis, humanismo y conductual. En la región latinoamericana, la homofobia es un fenómeno concebido aún como tabú, diferentes víctimas de este tipo de discriminación han recibido por parte de los victimarios agresiones de diversa índole. Estos aspectos pueden explicarse por efectos del reforzamiento de conductas por parte de la familia, grupo de pares y diversas representaciones sociales en el plano biopsicosocial. En síntesis, la homofobia es una problemática real que a la fecha perturba la salud mental y física de personas homosexuales, al provenir de un sistema en donde la homogeneidad y la “normalidad” es lo prioritario y fundamental para encajar dentro de una sociedad.

    8. LA GRAMÁTICA DESDE 1973 HASTA 2010

      Manuel Marticorena Quintanilla

      2014-12-01

      Full Text Available En el presente trabajo, hacemos una reseña de dieciocho textos de gramática difundidas en el Perú desde el año 1973. Iniciando con la publicación del Esbozo de una Nueva Gramática de la Lengua Española, que marca los cambios en los estudios gramaticales, pasando luego por textos excelentes como la Gramática Española de Juan Alcina Franch y José Manuel Blecua. En el Perú es un texto innovador la obra de Teodoro Ortiz Dueñas: Gramática Estructural. Ortografía. Composición, así como el de Luis Hernán Ramírez Introducción en la Gramática del Español Contemporáneo  (1984; y marca un claro hito en el estudio de la gramática estructural lindante con la generativa El Grupo Océano con su publicación la Enciclopedia Didáctica de Gramática (2002. Finalizamos con la Nueva Gramática de la lengua española (2010 de la Real Academia Española, integrada por dos tomos.

    9. Comprendiendo el community-based tourism desde la comunidad

      Esteban Ruiz

      2008-01-01

      Full Text Available El creciente impulso del Community-based tourism (CBT como vía para un turismo sostenible y estrategia para el desarrollo social nos obliga a profundizar en su comprensión. En este artículo proponemos como táctica teórico-metodológica la focalización analítica en la comunidad. El referente empírico de la investigación es el turismo comunitario (TC en Ecuador, donde se han seleccionado cinco comunidades para llevar a cabo un estudio etnográfico en profundidad. Como conclusión planteamos un marco comprensivo del TC que tiene tres pilares básicos: la centralidad analítica de las comunidades, la consideración del TC como `traducción´ antes que como `adaptación´ al mercado, y el carácter fortalecedor —antes que debilitador— del TC para las comunidades. De aquí se derivan una serie de indicadores cualitativos que sirven para encarar, desde el punto de vista teórico, la comprensión general del CBT y asimismo son útiles para la evaluación de la sostenibilidad de proyectos y experiencias de CBT

    10. La salud mental desde la transdisciplinariedad y el modelo integral

      José Domingo Flórez Moreno

      2011-01-01

      Full Text Available Se trata sobre la transdisciplinariedad, con el objetivo de analizar la temática y tener más elementos para el abordaje de la salud mental desde la integralidad en la psicología, teniendo como base el modelo integral de Ken Wilber. Para tal fin se realizó la búsqueda de información con base en seis ejes temáticos: salud mental, transdisciplinariedad, diferentes áreas de la salud mental y sus contribuciones, el modelo integral, nuevos paradigmas y la psicología integral. Se revisaron 89 artículos y 23 capítulos de libros publicados entre 2000 y 2010. Finalmente se reflexiona respecto a la identidad del sujeto en el nuevo paradigma, teniendo en cuenta al mediador,el cual interactúa entre la mente y la materia, la energía sutil y el cuerpo en la salud mental.

    11. El "régimen dual" en Israel desde 1967

      Lev Grinberg

      2008-01-01

      Full Text Available Este artículo aborda el establecimiento del peculiar régimen de dominación dual de Israel desde 1967, argumentando que la estructura de este régimen convierte a las élites militares en un actor político crucial. El régimen dual se basa en la separación geográfi ca entre dos regímenes diferentes de control y legitimación. Argumentaré aquí que la guerra de 1967 fue un parteaguas histórico, ya que acarreó un desdibujamiento de las fronteras israelíes y la llegada de un régimen dual que legitima la división del poder político entre las élites militares y civiles que gobiernan Israel-Palestina. Mi objetivo es mostrar las contradicciones inherentes de este régimen dual de “ocupación democrática” y así arrojar luz sobre la dinámica de los espacios políticos de acuerdo a la población ocupada: su apertura, por reconocimiento y negociación, y su cancelación, por el uso de la violencia.

    12. ILUMINANDO A LOS AGLOMERADOS PRODUCTIVOS DESDE LA FILOSOFÍA

      Marsanasco, Ana María

      2013-01-01

      Full Text Available Durante las últimas décadas se ha observado en la Argentina el desarrollo de estructuras asociativas caracterizadas por la integración de actores empresariales y no empresariales. Como parte de este proceso, surgen los aglomerados productivos y, conjuntamente, un marco teórico que da respuesta a las especificidades y demandas de este tipo de organizaciones.Este corpus teórico, denominado enfoque tradicional, reúne modelos y teorías que establecen la formación y desarrollo de los aglomerados productivos en Argentina. Es decir, es el paradigma predominante desde el cual se ilumina a estas organizaciones.En este contexto, el presente trabajo expone los principios de la teoría elaborada a fin de comprender las representaciones que los actores empresariales de las Pequeñas y Medianas Empresas (PyMEs construyen acerca de la conformación y morfología del aglomerado productivo que integran, y su relación con la generación de innovaciones. Estos principios comprenden las perspectivas epistemológicas de Cornelius Castoriadis y Martin Heidegger.

    13. Volatile metabolites from actinomycetes

      Scholler, C.E.G.; Gurtler, H.; Pedersen, R.;

      2002-01-01

      Twenty-six Streptomyces spp. were screened for their volatile production capacity on yeast starch agar. The volatile organic compounds (VOCs) were concentrated on a porous polymer throughout an 8-day growth period. VOCs were analyzed by gas chromatography with flame ionization detection and...

    14. Volatile metabolites from actinomycetes

      Scholler, C.E.G.; Gurtler, H.; Pedersen, R.; Molin, Søren; Wilkins, K.

      2002-01-01

      Twenty-six Streptomyces spp. were screened for their volatile production capacity on yeast starch agar. The volatile organic compounds (VOCs) were concentrated on a porous polymer throughout an 8-day growth period. VOCs were analyzed by gas chromatography with flame ionization detection and ident...

    15. Estudio de la posible participación de la corriente IKATP en las modificaciones que sobre estabilidad y heterogeneidad miocárdica produce el ejercicio físico crónico, en corazón aislado, normalmente oxigenado y tras isquemia regional aguda.

      Koninckx Cañada, Manuel

      2014-01-01

      El objetivo de la presente Tesis Doctoral ha sido valorar la posible participación del bloqueo del canal KATP en las modificaciones electrofisiológicas intrínsecas producidas por el ejercicio físico crónico, tanto en circunstancias de normal perfusión como, especialmente, durante la isquemia regional aguda. Para cumplimentar el objetivo comentado, nos hemos propuesto analizar, en corazón aislado y perfundido de conejo, las modificaciones producidas por la realización de ejercicio físico cróni...

    16. Integrones y su relación con el fenotipo de resistencia en bacilos gramnegativos aislados en el Hospital Torres Galdames de Iquique, Chile Integrons and their relationship with resistance phenotype in Gram negative bacilli isolated in the Hospital Torres Galdames, Iquique, Chile

      Rubén Moraga M; Edgardo Santander P; Teresa Arias C; Fermín Méndez A

      2007-01-01

      La resistencia antimicrobiana es codificada por algunos elementos genéticos que generan un flujo horizontal, particularmente, en ambientes que están sometidos a una fuerte presión selectiva, como ocurre en el ambiente hospitalario. En tal sentido, los bacilos gramnegativos, en el último tiempo, han cobrado importancia como agentes de infección nosocomial. Objetivo. Investigar la presencia de integrones en aislados clínicos de bacilos gramnegativos y su relación con el fenotipo de resistencia,...

    17. Democracia y partidos: el caso italiano desde una perspectiva comparativa Democracia y partidos: el caso italiano desde una perspectiva comparativa

      Oreste Massari

      2010-03-01

      Full Text Available La democracia italiana –desde después de la segunda guerra mundial hasta hoy– ha estado marcada, para bien y para mal, por el papel dominante de los partidos políticos. La relación entre democracia y partidos políticos se analiza a nivel teórico, comparativo e histórico. Concretamente se analiza la difícil transición, que todavía hoy está en marcha, desde la democracia proporcional (1945-1992 caracterizada primero por los partidos de masas y más tarde por la partitocracia, hacia la democracia mayoritaria (1994-, caracterizada primero por el bipolarismo de coalición y más tarde por el intento de conformar partidos mayoritarios. La última parte del artículo se detiene en analizar críticamente el PDL (Popólo della Libertá, dominado por el populismo de Berlusconi y el PD (Partito Democrático, planteado inicialmente como partido del líder y por esa razón todavía en crisis.The Italian democracy –from the second post-war period to today– has been marked, in the good and in the evil, from the dominant role of the political parties. The relationship between Italian democracy and political parties is analyzed on the theoretical, comparative and historical ground. Particularly the difficult passage is examined, still in progress, from the proportional democracy (1945-1992, characterized by the mass parties before and the partitocracy then, to the majority democracy (1994-, characterized before by the coalition bipolarism and by the attempt to build majority parties in recent times. In the last part of the article the PDL (People of the Liberty, dominated by the populism of Berlusconi, and the PD (Democratic Party, initially planned as party of the leader and for this still in crisis, are critically examined.

    18. Identification and preliminary characterization of non-polyene antibiotics secreted by new strain of actinomycete isolated from sebkha of Kenadsa, Algeria

      Omar; Messaoudi; Mourad; Bendahou; Ibrahim; Benamar; Djamal-Elddine; Abdelwouhid

      2015-01-01

      Objective: To study the antimicrobial activity of actinomycetes isolated from sabkha of Kenadsa and identification of the isolate interesting. Methods: Eighteen strains were isolated, using four culture media from sebkha of Kenadsa(Bechar, Southwestern Algeria). Screening of antimicrobial activity consisted of two steps: in primary screening, antibacterial activity was determined by using the agar plug method against test strains; in secondary screening, better isolate which showed a good activity in the first screening was selected to extract antimicrobial substances. The antimicrobial activities of extracts were evaluated by using Kirby-Bauer disc diffusion method. Partial characterization of antimicrobial products was performed on the basis of chemical revelations, UV-vis spectrometry and infrared spectroscopy. The identification of isolate interesting was performed through morphological, chemical, biochemical and physiological characteristics. Results: All isolates showed antimicrobial activity against at least one microorganism test. One isolate, LAM143 cG 3, was selected for its broad spectrum and high antimicrobial activity. The isolate LAM143 c G3 was identified as Spirillospora sp. The comparison between the species of this genus(Spirillospora rubra and Spirillospora albida) and our isolate indicated the existence of several physiological and biochemical differences which led us to suppose that this was a new member of this genus. Primary characterization of antimicrobial substances produced by the isolate LAM143 c G3 indicated the presence of amines and phenols. The UV–vis spectrum suggested a non-polyenic nature of substances secreted by our isolate, while infrared confirmed the presence of amine groups.Conclusions: The result of the present study revealed that sebkha of Kenadsa was rich in rare actinomycetes, that secreted interesting antimicrobial substance.

    19. Description of Kibdelosporangium banguiense sp. nov., a novel actinomycete isolated from soil of the forest of Pama, on the plateau of Bangui, Central African Republic.

      Pascual, Javier; González, Ignacio; Estévez, Mar; Benito, Patricia; Trujillo, Martha E; Genilloud, Olga

      2016-05-01

      A novel actinomycete strain F-240,109(T) from the MEDINA collection was isolated from a soil sample collected in the forest of Pama, on the plateau of Bangui, Central African Republic. The strain was identified according to its 16S rRNA gene sequence as a new member of the genus Kibdelosporangium, being closely related to Kibdelosporangium aridum subsp. aridum (98.6 % sequence similarity), Kibledosporangium phytohabitans (98.3 %), Kibdelosporangium aridum subsp. largum (97.7 %), Kibdelosporangium philippinense (97.6 %) and Kibledosporangium lantanae (96.9 %). In order to resolve its precise taxonomic status, the strain was characterised through a polyphasic approach. The strain is a Gram-stain positive, aerobic, non-motile and catalase-positive actinomycete characterised by formation of extensively branched substrate mycelia and sparse brownish grey aerial mycelia with sporangium-like globular structures. The chemotaxonomic characterisation of strain F-240,109(T) corroborated its affiliation into the genus Kibdelosporangium. The peptidoglycan contains meso-diaminopimelic acid; the major menaquinone is MK-9(H4); the phospholipid profile contains high amounts of phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol and an unidentified phospholipid; and the predominant cellular fatty acid methyl esters are iso-C16:0, iso-C14:0, iso-C15:0 and 2OH iso-C16:0. However, some key phenotypic differences regarding to its close relatives and DNA-DNA hybridization values indicate that strain F-240,109(T) represents a novel Kibdelosporangium species, for which the name Kibdelosporangium banguiense sp. nov. is proposed. The type strain is strain F-240,109(T) (=DSM 46670(T), =LMG 28181(T)). PMID:26936255

    20. Genome-wide analysis of the role of GlnR in Streptomyces venezuelae provides new insights into global nitrogen regulation in actinomycetes

      Bibb Mervyn J

      2011-04-01

      Full Text Available Abstract Background GlnR is an atypical response regulator found in actinomycetes that modulates the transcription of genes in response to changes in nitrogen availability. We applied a global in vivo approach to identify the GlnR regulon of Streptomyces venezuelae, which, unlike many actinomycetes, grows in a diffuse manner that is suitable for physiological studies. Conditions were defined that facilitated analysis of GlnR-dependent induction of gene expression in response to rapid nitrogen starvation. Microarray analysis identified global transcriptional differences between glnR+ and glnR mutant strains under varying nitrogen conditions. To differentiate between direct and indirect regulatory effects of GlnR, chromatin immuno-precipitation (ChIP using antibodies specific to a FLAG-tagged GlnR protein, coupled with microarray analysis (ChIP-chip, was used to identify GlnR binding sites throughout the S. venezuelae genome. Results GlnR bound to its target sites in both transcriptionally active and apparently inactive forms. Thirty-six GlnR binding sites were identified by ChIP-chip analysis allowing derivation of a consensus GlnR-binding site for S. venezuelae. GlnR-binding regions were associated with genes involved in primary nitrogen metabolism, secondary metabolism, the synthesis of catabolic enzymes and a number of transport-related functions. Conclusions The GlnR regulon of S. venezuelae is extensive and impacts on many facets of the organism's biology. GlnR can apparently bind to its target sites in both transcriptionally active and inactive forms.

    1. Identification and characterization of a new erythromycin biosynthetic gene cluster in Actinopolyspora erythraea YIM90600, a novel erythronolide-producing halophilic actinomycete isolated from salt field.

      Dandan Chen

      Full Text Available Erythromycins (Ers are clinically potent macrolide antibiotics in treating pathogenic bacterial infections. Microorganisms capable of producing Ers, represented by Saccharopolyspora erythraea, are mainly soil-dwelling actinomycetes. So far, Actinopolyspora erythraea YIM90600, a halophilic actinomycete isolated from Baicheng salt field, is the only known Er-producing extremophile. In this study, we have reported the draft genome sequence of Ac. erythraea YIM90600, genome mining of which has revealed a new Er biosynthetic gene cluster encoding several novel Er metabolites. This Er gene cluster shares high identity and similarity with the one of Sa. erythraea NRRL2338, except for two absent genes, eryBI and eryG. By correlating genotype and chemotype, the biosynthetic pathways of 3'-demethyl-erythromycin C, erythronolide H (EH and erythronolide I have been proposed. The formation of EH is supposed to be sequentially biosynthesized via C-6/C-18 epoxidation and C-14 hydroxylation from 6-deoxyerythronolide B. Although an in vitro enzymatic activity assay has provided limited evidence for the involvement of the cytochrome P450 oxidase EryFAc (derived from Ac. erythraea YIM90600 in the catalysis of a two-step oxidation, resulting in an epoxy moiety, the attempt to construct an EH-producing Sa. erythraea mutant via gene complementation was not successful. Characterization of EryKAc (derived from Ac. erythraea YIM90600 in vitro has confirmed its unique role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide. Genomic characterization of the halophile Ac. erythraea YIM90600 will assist us to explore the great potential of extremophiles, and promote the understanding of EH formation, which will shed new insights into the biosynthesis of Er metabolites.

    2. CONOCIMIENTO, ECONOMÍA, DESARROLLO Y SOCIEDAD: TRAZOS DESDE LA COMPLEJIDAD

      Ricardo Guzmán Díaz; Aurora Adriano Anaya

      2013-01-01

      Ante el surgimiento en las últimas décadas de las llamadas economías basadas en el conocimiento, han aparecido diversas propuestas explicativas o marcos teóricos para la comprensión de las mismas. En este trabajo tenemos el propósito de reflexionar, con una visión humanística como eje conductor, en torno a diversos conceptos introducidos por pensadores como Dominique Foray, desde la teoría económica, Javier Echeverría, desde la filosofía de la ciencia, Manuel Castells, desde la sociología y E...

    3. Gestionar cambios desde la óptica cultural

      Ramón Antonio Piña Rodríguez

      2005-01-01

      Full Text Available Se proponen enfoques de cómo abordar los cambios organizacionales en las pequeñas y medianas empresas cubanas (PYMEs desde la óptica cultural. La experiencia y conocimientos acumulados en los servicios de consultoría a empresas cubanas y relativos a la implementación de nuevas herramientas gerenciales, -muy a tono con el desarrollo de la espiral evolutiva de la economía- , muestran la necesidad de enfatizar la óptica cultural en el ámbito de las pequeñas y medianas empresas cubanas. Se describe el contexto empresarial cubano abordado en calidad de objeto de consultorías, la relación y correspondencia de las teorías del cambio y enfoques culturales con la teoría marxista, así como la introducción de la propia técnica descriptiva cultural ya aplicada con éxito en empresas cubanas. Además, son abordados conceptos primarios imprescindibles para la comprensión del cambio organizacional, el reconocimiento de su existencia y necesidad de gerenciarlo a partir del dominio de los rasgos culturales propios que apalancan o pueden constituir barreras para el cambio. Describe el artículo y responde además en calidad de soporte académico, al curso de post grado asociado a la introducción de la consultoría gerencial .Es intención de los autores argumentar, promover y presentar al empresariado cubano las herramientas que le permitan asumir su propia capacidad de cambio en las organizaciones que dirigen, minimizando los costos materiales y emocionales que cada proceso de cambio contrae.

    4. La violencia escolar desde la infancia hasta la juventud

      Laura Oliva-Zárate

      2013-12-01

      Full Text Available La violencia escolar se ha convertido en foco de atención no sólo de investigadores, sino de todos aquellos involucrados en el quehacer educativo y de la sociedad en general. Lo anterior debido a la creciente ola de violencia que se presenta en los distintos ámbitos de la actividad humana. El punto de interés del presente estudio tiene como objetivo la descripción de la violencia narrada por los mismos alumnos de los distintos niveles educativos. Para ello, se utilizó la técnica de grupo de discusión para rescatar los discursos individuales y colectivos de 37 estudiantes (16 mujeres y 21 varones entre 3 y 22 años provenientes de 27 escuelas de diversos niveles educativos de la ciudad de Xalapa, México. El análisis cualitativo de los datos se realizó mediante el programa ATLAS.ti. Los resultados indicaron la existencia de violencia en todos los niveles escolares estudiados. Sin embargo, las formas, tipos, lugares y la dirección de la violencia, así como el género en la expresión de la violencia cambió desde niños preescolares hasta jóvenes universitarios. Los resultados señalaron que con respecto a la dirección de la violencia, el maltrato entre iguales se presentó con mayor frecuencia al igual que la violencia de tipo psicológico. Respecto a las formas predominaron los golpes y las agresiones verbales con manifestación principalmente en el baño y los espacios abiertos de la escuela. Los datos encontrados en cada nivel escolar permiten concluir que el comportamiento violento se encuentra relacionado con la edad y que junto con la influencia cultural determinan su expresión.

    5. Comprensión de fenómenos naturales desde la perspectiva científica desde el contexto ecosistemas

      Zepeda Navarrete, José Alejandro; Zacarías Pérez, Patricia; Ramírez Ponce, Ramón; Martínez Díaz, Francisco René; Reyes Matias, Danilka

      2012-01-01

      • Información General • Evaluación Diagnóstica • Contenido o Tema 1 - ¿Qué significa la competencia comprensión de fenómenos y procesos naturales desde la perspectiva científica en el contexto de los ecosistemas?  Objetivos específicos del tema  Actividad inicial del tema  Desarrollo del tema  Actividad final del tema  Recursos adicionales o Tema 2 - ¿Por qué es relevante que adquiera la competencia comprensión de fenómenos y procesos naturales desde la perspec...

    6. La subjetividad desde la perspectiva histórico cultural: un tránsito desde el pensamiento dialéctico al pensamiento complejo.

      Oscar Hernández

      2009-01-01

      En el presente artículo se expone conceptualmente la categoría de la subjetividad y los elementos que la sustentan desde las propuestas provenientes de la psicología histórico cultural. Para ello se hizo una revisión de las principales ideas de la filosofía del materialismo dialéctico, y de los elementos en la obra de L.Vigotsky que se relacionancon su develamiento. Adicionalmente, se realizó una breve exposición de las contribuciones más destacadas que desde la psicología soviética, facilita...

    7. Contribución al conocimiento de la morfología y taxonomía de un hongo del genero Prototrichia aislado de un cultivo de rosas

      Correa de Restrepo Marina

      1996-06-01

      Full Text Available The macroscopic and microscopic morfology of an isolated fungus of rose cultivation were studied. It to find in fructification tipic form by half direct observation of macroscopic reproduction structure, this factor assign high stetic inconvenience for products commercial presentation and accept. After a review of the taxonomic literature, we placed it in the Prototrichia genus, of class mixomycetes, but not a particular species, for absence of necesary support to moment. We used the spore to spore technique add the nutritions cover-glass technique to secure the spore transfer and germination, and fungus development for the morfologic description; and by mounting in cover-glass of microscopic structure to study.Se estudio la morfología tanto macro como microscópica de un hongo aislado de plantas de rosas. Se encontró la fructificacion correspondiente a la forma asexual típica de la especie del género Prototrichia, no se conoce su papel ecológico, sinembargo podría afectar la presentación adecuada y para la aceptación a nivel comercial de las rosas. A partir de las muestras colectadas se realizaron estudios para su clasificación. Revisada la literatura pertinente se ubico dentro del genero Prototrichia de la clase Mixomycetes, pero no dentro de alguna especie en particular, por no disponerse de momento de las ayudas necesarias para tal fin; de este hongo no se tienen registros para el neotropico (Según Farr, M. L., 1976.Se siguieron las siguientes técnicas: Siembra de esporas (Espora a Espora, en medios de Agar - avena; siembra en lámina impregnada de material nutritivo para asegurar el traspaso y germinación de las esporas y desarrollo del hongo para su descripción morfológica microscópica, y se hicieron montajes en laminas de las estructuras a estudiar para la descripción microscópica.

    8. Pensar la Complejidad desde la praxis cognoscente de la racionalidad intersubjetiva.

      Álvaro B. Márquez-Fernández

      2007-01-01

      En este ensayo se aborda la transdisciplinariedad desde la perspectiva de la Complejidad se destaca el supuesto filosófico que permite pensar la transdisciplinariedad desde una relación epistémica mucho más holística y contingente, entre el sujeto conociente y el objeto cognitivo, que nos permita desfundamentar los principios positivistas de la razón moderna.

    9. Astronomía Gamma desde El Leoncito

      Rovero, A. C.; Colombo, E.; Sahade, J.; Weekes, T. C.

      La astronomía gamma, en el extremo de energías detectadas hasta el presente, se encuentra en desarrollo en muchas partes del mundo y proporciona resultados sorprendentes a medida que la tecnología avanza en el desarrollo de nuevos detectores. La técnica Cherenkov Atmosférica es la utilizada para la detección, mediante telescopios en tierra, del efecto secundario causado por la radiación gamma de muy altas energías (E ≈ TeV). Por medio de telescopios o arreglos de detectores y utilizando la técnica mencionada, se han podido detectar algunas fuentes puntuales de mucha importancia astrofísica como la Nebulosa del Cangrejo y la galaxia de núcleo activo Markarian 421. El sistema instalado en San Juan, en cooperación con el grupo de Radiación Gamma del Observatorio Whipple, está constituído por tres colectores de 1.5m que registran pulsos de luz generados por las cascadas de partículas producidas por rayos cósmicos de todo tipo al entrar en la atmósfera terrestre. El sistema opera por barrido en ascención recta de la fuente observada que, para esta etapa del experimento, es el Centro Galáctico. Esta fuente extensa es conocida como emisor gamma de energías inferiores al TeV y ha sido observada anteriormente en este rango de energía desde el hemisferio norte pero con elevaciones bajas, situación no óptima para este tipo de técnica. Se presentan los primeros resultados del análisis de los datos obtenidos observando la región del Centro Galáctico, luego de dos años de observaciones, así como una descripción de la técnica Cherenkov Atmosférica y del equipo utilizado en la experiencia.

    10. 生防放线菌TGNBSA5的鉴定及其活性物质的研究%Identification of a Bioactive Endophytic Actinomycete TGNBSA5 and Study on Its Active Substances

      黄以超; 颜霞; 王建华; 高小宁; 王惠; 黄丽丽

      2012-01-01

      Endophytic actinomycete TGNBSA5, isolated from Arctium lappa stem, has strong inhibitory activity against Pseudotnonas syringae pv, actinidiae (Psa). In order to exploit the bio-control application of the actinomycete, physiological and biochemical characterization, morphological, and 16S rDNA sequence analysis were conducted to identify the strain. Meanwhile, after further fermentation of the strain, bioactive compounds were isolated and purified by ethyl acetate extraction, chro-matographic technology, and HPLC detection. The result showed that the endophytic actinomycete was classified as Streptomyces sporovirgulis. NMR and UV spectra analysis indicated that one of the bioactive compounds was benzyl alcohol. It is the first time that the endophytic actinomycete Streptomyces sporovirguiis be isolated from plant. The clarification of the bioactive benzyl alcohol provides theoretical basis for the development of this actinomycete and the bio-control of kiwifruit canker disease as well.%牛蒡内生放线菌TGNBSA5对猕猴桃溃疡病菌有较好拮抗作用.为开发该生防菌的生物防治价值,采用生理生化活性测定、形态观察及16S rDNA序列分析,并经发酵培养后,发酵液采用乙酸乙酯萃取、柱层析和薄层层析、HPLC检测等方法分离纯化抑菌活性组分.结果表明,该内生放线菌为链霉菌属的孢杆链霉菌( Streptomyces sporovirgulis),经紫外和NMR鉴定活性组分之一为苯甲醇.孢杆链霉菌为第一次从植物中分离获得,且活性组分苯甲醇的明确将为猕猴桃溃疡病的防治提供理论依据.

    11. Frecuencia de genes que codifican factores de virulencia en Staphylococcus aureus aislados de niños que concurrieron al Hospital General Pediátrico Niños de Acosta Ñú, durante el año 2010

      Fatima Rodríguez Acosta

      2015-04-01

      Full Text Available Staphylococcus aureus es un microorganismo con habilidad de infectar diferentes tejidos celulares, por portar genes que le confieren resistencia a antibióticos, factores de virulencia y su plasticidad genética, que podrían contribuir a una progresión rápida y complicada de la enfermedad. El Paraguay no cuenta con datos epidemiológicos que indiquen los factores de virulencia que presentan las cepas de S. aureus, por lo que el objetivo del trabajo fue determinar un perfil de virulencia detectando los genes codificantes de: hemolisinas α y β, enterotoxinas A, B, C, D, H y toxinas exfoliativas A y B. Este estudio observacional descriptivo de corte transverso, con muestreo no probabilístico de casos consecutivos, incluyó 50 aislados de S. aureus obtenidos a partir de muestras clínicas de secreciones de piel, partes blandas o líquidos corporales de pacientes menores de 17 años que concurrieron al Hospital General Pediátrico Niños de Acosta Ñú durante el año 2.010. Las reacciones de PCR incluyeron la detección de los genes: sea+seb+sec+ADNr16S, hlA+hlB, eta+etb, sed y seh. El 82% de los aislados provenía de niños que presentaron cuadros clínicos compatibles con infecciones de piel y partes blandas y el 18% de cuadros clínicos graves como sepsis, osteomielitis y neumonías. Los aislados contaban con datos de portación de Leucocidina de Panton-Valentine, el cual fue el factor de virulencia más frecuentemente detectado (58%, seguido de las hemolisinas alfa (16% y beta (8%. Las enterotoxinas y las toxinas exfoliativas fueron menos frecuentes (0-2%, y no se detectaron genes codificantes de las enterotoxinas C y D.

    12. An actinomycete isolate from solitary wasp mud nest having strong antibacterial activity and kills the Candida cells due the shrinkage and the cytosolic loss

      Vijay eKumar

      2014-08-01

      Full Text Available An actinomycetes strain designated as MN 2(6 was isolated from the solitary wasp mud nest. The isolate was identified using polyphasic taxonomy. It produced the extensive branched brown substrate and white aerial hyphae that changed into grayish black. The aerial mycelia produced the spiral spore chains with rugose spore surface. The growth was observed between temperature range of 27-37°C, pH 8-10 and below salt concentration of 6% (w/v. The comparative analysis of 16S rRNA gene sequence and phylogenetic relationship showed that strain MN 2(6 lies in clade with Streptomyces hygroscopicus subsp. hygroscopicus NRRL 2387T, Streptomyces sporocinereus NBRC 100766T and Streptomyces demainii NRRL B-1478T with which it shares a 16S rRNA gene sequence similarity of 99.3%. The strain MN 2(6 can be differentiated from type strains based on phenotypic characteristics. The strain MN 2(6 showed most promising activity against Gram-positive, Gram-negative bacteria, acid-fast bacilli and Candida species suggesting broad-spectrum characteristics of the active metabolite. Evaluation of anti-candidal activity of the metabolite of strain MN 2(6 by scanning electron microscopy (SEM revealed changed external morphology of yeast. It kills the Candida cells due to the shrinkage and the cytosolic loss. However, further studies are required to elucidate the structure of the active metabolite produced by the isolate MN 2(6

    13. Biocontrol of Rhizoctonia solani damping-off and promotion of tomato plant growth by endophytic actinomycetes isolated from native plants of Algerian Sahara.

      Goudjal, Yacine; Toumatia, Omrane; Yekkour, Amine; Sabaou, Nasserdine; Mathieu, Florence; Zitouni, Abdelghani

      2014-01-20

      Thirty-four endophytic actinomycetes were isolated from the roots of native plants of the Algerian Sahara. Morphological and chemical studies showed that twenty-nine isolates belonged to the Streptomyces genus and five were non-Streptomyces. All isolates were screened for their in vitro antifungal activity against Rhizoctonia solani. The six that had the greatest pathogen inhibitory capacities were subsequently tested for their in vivo biocontrol potential on R. solani damping-off in sterilized and non-sterilized soils, and for their plant-growth promoting activities on tomato seedlings. In both soils, coating tomato seeds with antagonistic isolates significantly reduced (Pdamping-off of tomato seedlings. Among the isolates tested, the strains CA-2 and AA-2 exhibited the same disease incidence reduction as thioperoxydicarbonic diamide, tetramethylthiram (TMTD) and no significant differences (Pseed-treated seedlings compared to the control. The taxonomic position based on 16S rDNA sequence analysis and phylogenetic studies indicated that the strains CA-2 and AA-2 were related to Streptomyces mutabilis NBRC 12800(T) (100% of similarity) and Streptomyces cyaneofuscatus JCM 4364(T) (100% of similarity), respectively. PMID:23920229

    14. Microbispora sp. LGMB259 endophytic actinomycete isolated from Vochysia divergens (Pantanal, Brazil) producing β-carbolines and indoles with biological activity.

      Savi, Daiani C; Shaaban, Khaled A; Vargas, Nathalia; Ponomareva, Larissa V; Possiede, Yvelise M; Thorson, Jon S; Glienke, Chirlei; Rohr, Jürgen

      2015-03-01

      Endophytic actinomycetes encompass bacterial groups that are well known for the production of a diverse range of secondary metabolites. Vochysia divergens is a medicinal plant, common in the "Pantanal" region (Brazil) and was focus of many investigations, but never regarding its community of endophytic symbionts. During a screening program, an endophytic strain isolated from the V. divergens, was investigated for its potential to show biological activity. The strain was characterized as Microbispora sp. LGMB259 by spore morphology and molecular analyze using nucleotide sequence of the 16S rRNA gene. Strain LGMB259 was cultivated in R5A medium producing metabolites with significant antibacterial activity. The strain produced 4 chemically related β-carbolines, and 3 Indoles. Compound 1-vinyl-β-carboline-3-carboxylic acid displayed potent activity against the Gram-positive bacterial strains Micrococcus luteus NRRL B-2618 and Kocuria rosea B-1106, and was highly active against two human cancer cell lines, namely the prostate cancer cell line PC3 and the non-small-cell lung carcinoma cell line A549, with IC50 values of 9.45 and 24.67 µM, respectively. 1-Vinyl-β-carboline-3-carboxylic acid also showed moderate activity against the yeast Saccharomyces cerevisiae ATCC204508, as well as the phytopathogenic fungi Phyllosticta citricarpa LGMB06 and Colletotrichum gloeosporioides FDC83. PMID:25385358

    15. Switching antibiotics production on and off in actinomycetes by an IclR family transcriptional regulator from Streptomyces peucetius ATCC 27952.

      Chaudhary, Amit Kumar; Singh, Bijay; Maharjan, Sushila; Jha, Amit Kumar; Kim, Byung-Gee; Sohng, Jae Kyung

      2014-08-01

      Doxorubicin, produced by Streptomyces peucetius ATCC 27952, is tightly regulated by dnrO, dnrN, and dnrI regulators. Genome mining of S. peucetius revealed the presence of the IclR (doxR) type family of transcription regulator mediating the signal-dependent expression of operons at the nonribosomal peptide synthetase gene cluster. Overexpression of doxR in native strain strongly repressed the drug production. Furthermore, it also had a negative effect on the regulatory system of doxorubicin, wherein the transcript of dnrI was reduced to the maximum level in comparision with the other two. Interestingly, the overexpression of the same gene also had strong inhibitory effects on the production of actinorhodin (blue pigment) and undecylprodigiosin (red pigment) in Streptomyces coelicolor M145, herboxidiene production in Streptomyces chromofuscus ATCC 49982, and spinosyn production in Saccharopolyspora spinosa NRRL 18395, respectively. Moreover, DoxR exhibited pleiotropic effects on the production of blue and red pigments in S. coelicolor when grown in different agar media, wherein the production of blue pigment was inhibited in R2YE medium and the red pigment was inhibited in YEME medium. However, the production of both blue and red pigments from S. coelicolor harboring doxR was halted in ISP2 medium, whereas S. coelicolor produced both pigmented antibiotics in the same plate. These consequences demonstrate that the on and off production of these antibiotics was not due to salt stress or media compositions, but was selectively controlled in actinomycetes. PMID:24786531

    16. La Educación Física [Escolar]: reflexiones desde la Gimnasia

      Meneses, Gastón

      2013-01-01

      Este trabajo intenta abordar el problema acerca de la educación física en relación al objeto de estudio y a la especificidad del campo, a partir de una reflexión desde la práctica. La educación física como práctica pedagógica se legitima desde su origen en el seno de la institución escolar. Es decir, hay educación física porque hay escuela. O dicho de otra manera, si hay algo que define a la educación física desde su génesis, es su carácter de escolar. ¿Pero de qué concepto de escuela h...

    17. LA ENSEÑANZA DE LENGUAS EXTRANJERAS DESDE LA PERSPECTIVA DE LA TEORÍA VIGOTSKIANA

      Raúl Conrado Sánchez Cortina

      2014-09-01

      Full Text Available La teoría vigotskiana y sus ideas esenciales sobre la formación de la personalidad humana son tomadas como punto de partida en el presente artículo, en el cual se realiza un análisis de esas ideas, las que van dando sustento a diversas teorías referidas a la enseñanza de idiomas extranjeros desde el llamado “Enfoque cognitivo, comunicativo y sociocultural”, o desde los matices que a esta enseñanza se ofrece a partir de la “Didáctica interactiva”. En el presente artículo se revelan otras concepciones que intervienen en el proceso de la enseñanza de idiomas y que están estrechamente vinculadas con la identidad cultural de los actores del proceso y los valores que llegan desde la nueva lengua que se estudia.

    18. EL ANÁLISIS DE COYUNTURA DESDE LA PERSPECTIVA DE UNA ECONOMÍA PARA LA VIDA

      Henry Mora Jiménez

      2007-01-01

      Resumen Analizar la coyuntura económica, social y ambiental desde la perspectiva de una economía para la vida exige objetividad científica y un compromiso político con el bien común. Además, no es un análisis desde “lo económico” o desde “lo social”. Se trata de un análisis desde los efectos (directos e indirectos, intencionales y no intencionales) que las tendencias hacia la irracionalidad del sistema capitalista provocan sobre el ser humano y el medio ambiente. Hablamos de estos efectos com...

    19. Diferencia y otredad desde la fenomenología de Husserl

      Rosemary Rizo-Patrón

      2010-01-01

      El tema del “pluralismo”, frecuentemente abordado desde la sola perspectiva de los retos éticos, políticos y culturales que la era de la globalización plantea a las comunidades humanas en todo el orbe, nos enfrenta a profundos dilemas racionales sobre los que ha reflexionado la humanidad desde la aurora de la filosofía griega. El texto se refiere a dos de ellos: a la relación, por un lado, entre unidad y multiplicidad; y, por el otro, entre “semejanza” y “otredad”, a saber, entre “identidad” ...

    20. El clima organizacional en universidades de Bogotá desde la perspectiva de los estudiantes

      José Javier Bermudez-Aponte; Alexandra Pedraza Ortiz; Clara Inés Rincón Rivera

      2015-01-01

      Esta investigación planteó como objetivo caracterizar el clima organizacional de seis universidades, localizadas en la ciudad de Bogotá Distrito Capital (D.C.), Colombia, a partir de las percepciones expresadas por algunos de sus estudiantes. La metodología se abordó desde el enfoque cualitativo, con alcance descriptivo, busca comprender el fenómeno objeto de análisis desde la visión de conjunto, las posibilidades de análisis de lo subyacente y el sentido de construcción del objeto. Los halla...

    1. El estudio de la infancia desde la psicología cultural: un encuentro de perspectivas

      Juan José Yoseff Bernal

      2005-01-01

      El estudio cultural de la infancia parte de la premisa de que cultura y mente se relacionan estrechamente, en una relación de constitución mutua. Implica que el infante es un ser activo desde que nace (quizá desde antes) y que en interacción con los otros, sus ‘próximos”, es que va constituyéndose su psiquismo. La forma particular que se vaya constituyendo a lo largo del desarrollo devendrá tanto de lo que el infante aporte como de lo que su entorno social le proporcione a través de las costu...

    2. Un juego de espejos : pensar la diferencia desde la pedagogía intercultural

      Eduardo S. VILA MERINO

      2012-01-01

      Con este artículo se pretenden aportar reflexiones que nos permitan pensar las diferencias que nos constituyen desde el tamiz intercultural para, a partir de su consideración, ver otras maneras de entender las relaciones educativas bajo los principios de la equidad y la inclusión. Para ello, en primer lugar se desgrana el concepto de diferencia en consonancia con el de identidad, de forma que obtengamos un sustrato teórico desde el que elevar nuestros análisis y propuestas. En segundo lugar s...

    3. LA DISCAPACIDAD COMO FENÓMENO SOCIAL: UN ACERCAMIENTO DESDE LA SOCIOLOGÍA

      Alexandro Rodríguez Lores

      2012-01-01

      El presente artículo aborda un acercamiento el estudio contemporáneo sobre discapacidad desde el punto de vista sociológico, explica este fenómeno como un hecho social, estudiado por su particularidad por varias ciencias y el aporte de algunas teorías que desde el funcionalismo, el estructural funcionalismo y el interaccionismo simbólico han pretendido a través de sus aristas explorar, explicar y analizar el mundo de la discapacidad. Se refleja que en discapacidad no todo esta plenamente solu...

    4. Los laboratorios de enseñanza desde una perspectiva histórica y social

      Santilli Traba, H.

      2009-01-01

      A partir de un estudio de caso (Física en Ingeniería), se indaga la evolución de los laboratorios de enseñanza, desde el marco de las olas históricas. El enfoque es cualitativo, holístico e interpretativo, se analizan procesos desde la perspectiva de los actores sociales involucrados. Se identifican los cambios en los objetivos y actividades planteados, con miras a desarrollar los conocimientos, científico e ingenieril, de los estudiantes. Se reconocen los cambios en los modelos de ciencia y ...

    5. La rentabilidad desde la organización industrial: Una revisión de trabajos

      Ortiz Correa, Javier Santiago

      2003-01-01

      Resumen:El tema de la rentabilidad ha sido abordado desde diferentes tradiciones del análisis económico, en el presente artículo se presenta el análisis, que sobre la rentabilidad, realiza la teoría de la organización industrial. La organización industrial es la parte de la economía que estudia como se organizan los productores en los mercados; se ha desarrollado desde las vertientes clásicas, que resaltan la importancia del análisis a nivel de la industria para explicar las diferencias en lo...

    6. LA ENSEÑANZA DE LENGUAS EXTRANJERAS DESDE LA PERSPECTIVA DE LA TEORÍA VIGOTSKIANA

      Raúl Conrado Sánchez Cortina; Raúl Sánchez Muñagorri

      2014-01-01

      La teoría vigotskiana y sus ideas esenciales sobre la formación de la personalidad humana son tomadas como punto de partida en el presente artículo, en el cual se realiza un análisis de esas ideas, las que van dando sustento a diversas teorías referidas a la enseñanza de idiomas extranjeros desde el llamado “Enfoque cognitivo, comunicativo y sociocultural”, o desde los matices que a esta enseñanza se ofrece a partir de la “Didáctica interactiva”. En el presente artículo se revelan otras conce...

    7. Trabajo social y salud mental. Intervención desde el Tercer Sector.

      Acinas Fernández, Patxi

      2014-01-01

      En los últimos años se ha producido un incremento del número de personas con enfermedad mental, como consecuencia de ello, se han visto aumentadas las necesidades tanto de atención, y cuidado como reivindicativas en pro de los derechos del colectivo. El trabajador/a Social, desde unos conocimientos y técnicas propias proporciona una atención integral, tanto a las personas afectadas como a sus familiares, realizando la intervención social desde las dimensiones micro como macro, y siendo esta l...

    8. Experiencias y vivencias del embarazo en adolescentes explicadas desde lo social

      VIRGINIA INÉS SOTO

      2008-12-01

      Full Text Available Describir y explicar desde lo social las experiencias y vivencias de las adolescentes embarazadas, como el contexto para una provisión de cuidado integral, individualizado y contextualizado, es necesario para la práctica de enfermería, y tratar este fenómeno como hecho social exige asimilar las realidades del mundo social a las del mundo exterior, adoptando el principio de que se ignora por completo lo que son, reconociendo que el hecho social no puede ser modificado por un simple decreto de la voluntad, sino que exige un esfuerzo laborioso, considerando la experiencia desde los ámbitos interno y externo. Presentar un marco conceptual y referencial desde lo social, interpretando la experiencia y la vivencia, permitirá a la enfermera comprender este fenómeno cuando dichas experiencias involucran o ponen en riesgo la salud de la adolescente gestante, máxime que la práctica clínica de enfermería se ha estudiado primariamente desde una perspectiva sociológica, pues comparte una importancia social destacada y un fin social, centrada en facilitar, apoyar y atender a los individuos, familias, comunidades y/o sociedades para realzar, mantener y recuperar la salud, y reducir y aliviar los efectos de la enfermedad, con el resultado explícito de promover la calidad de vida relacionada con la salud dentro de contextos ambientales inmediatos y más amplios.

    9. CONSTRUCCIÓN DE POLÍTICAS DE MEMORIA DESDE LA VIDA COTIDIANA

      María José Reyes Andreani

      2015-08-01

      Full Text Available En las últimas décadas, en aquellas sociedades occidentales que han enfrentado situaciones de violencia política, se ha generado un debate sobre los efectos políticos que implica recordar/olvidar dicho pasado. Es así como, las Ciencias Sociales desde los años 90, han hecho de las políticas de memoria un campo de estudios. El presente artículo tiene por objetivo sostener a través de un trabajo teórico, que las políticas de memoria, al contrario de lo que usualmente se plantea, no solo se producen desde acciones estratégicas emanadas de ámbitos políticos-institucionales, sino también desde acciones no planificadas y espontáneas que emergen desde el espacio cotidiano. Lo anterior se sustenta pues al recordar cotidianamente, junto con apelar al pasado en un escenario de conflictos, se producen posicionamientos que generan un ordenamiento de hegemonías y resistencias respecto a lo que se recuerda y al lugar que se le otorga en el presente.

    10. Algunas cuestiones prácticas de los arrendamientos financieros desde un punto de vista contable

      Tejada Ximénez de Olaso. Mª Carmen

      2013-01-01

      El presente artículo describe, utilizando casos prácticosy desde un punto de vista contable, algunas cuestiones para clarificar el estudio del arrendamiento financiero, bajo el el Plan Contable, aprobado por el Real Decreto 1514/2007 de 16 de noviembre.

    11. Algunas cuestiones prácticas de los arrendamientos financieros desde un punto de vista contable

      Tejada Ximénez de Olaso. Mª Carmen

      2013-01-01

      Full Text Available El presente artículo describe, utilizando casos prácticosy desde un punto de vista contable, algunas cuestiones para clarificar el estudio del arrendamiento financiero, bajo el el Plan Contable, aprobado por el Real Decreto 1514/2007 de 16 de noviembre.

    12. La Cultura de la Inteligencia desde la Perspectiva de la Investigación Histórica

      Goberna Falque, Juan

      2006-01-01

      Desde las más altas instancias del Centro Nacional de Inteligencia (CNI) se viene proponiendo desde hace algunos años la creación y difusión de algo que se ha dado en llamar la «cultura de la inteligencia». Como miembro de un equipo de investigaci

    13. Al este de la campana de Gauss. Una aproximación desde la docencia a las altas capacidades. Aprendizajes significativos desde su hipersensibilidad e hiperestesia

      Fernández Bascaran, Eva

      2013-01-01

      Mi investigación se basa en tratar de obtener aprendizajes significativos en alumnado con altas capacidades tomando como aliado a su favor su hipersensibilidad e hiperestesia, su alto sentido de la justicia y su empatía. Investigar la habilidad del alumnado con altas capacidades no solo en el plano cognitivo, sino también desde el plano sensitivo-afectivo-emocional. Utilizar su hipersensibilidad y empatía para ayudar a sus compañeros (aprendizaje cooperativo) y reforzar su personalidad y feli...

    14. Funcionamiento cognitivo en la vejez: desde el modelo bidimensional y desde el modelo de pensamiento crítico : Un estudio empírico

      Maccarone, Silvina; Monchietti, Alicia

      2009-01-01

      Este trabajo presenta resultados de un proyecto de investigación realizado durante el año 2008 en el marco de una Beca de investigación otorgada por la Universidad Nacional de Mar del Plata. El mismo forma parte de los proyectos que desarrolla el Grupo de Investigación “Temas de Psicología del Desarrollo” orientados a caracterizar la cognición y el pensamiento en la vejez. Aquí se explora el funcionamiento cognitivo en el envejecimiento normal, desde dos modelos teórico-empíricos: bidimension...

    15. Management y teoría conductista desde la perspectiva de las ciencias humanas: una mirada desde la interdisciplinariedad Conductist theory and management from the perspective of human science: A view from interdisciplinary

      Leonardo Solarte-Pazos

      2006-01-01

      El texto presenta una reflexión sobre el alcance del behaviorismo (conductismo) dentro del management, desde una perspectiva interdisciplinaria de las ciencias humanas que integra aspectos de la dimensión biológica y psicoafectiva del ser humano. Esta perspectiva de las ciencias humanas aporta un lente adecuado para analizar el management motivacional, no desde el discurso intelectual ni ideológico, sino desde la misma teoría científica que el conductismo pregona como su mayor soporte. El doc...

    16. La innovación tecnológica desde una perspectiva evolutiva

      Benavides Óscar A.

      2004-01-01

      Este artículo analiza el proceso de innovación tecnológica desde la economía política, específicamente, desde la perspectiva evolutiva.
      Se adopta el enfoque schumpeteriano en el cual el proceso de innovación tecnológica puede ser visto como un proceso de aprendizaje. En ese sentido, la innovación tecnológica representa un cambio en el conocimiento y, por ende, tiene un carácter epistemológico. Esta conceptualización, junto con el análisis histórico del proceso de innovación tecnoló...

    17. La deserción estudiantil UIS, una mirada desde la responsabilidad social universitaria

      Ruth Zárate Rueda

      2014-01-01

      Full Text Available La deserción estudiantil en las instituciones de educación superior se ha convertido en un problema social que aqueja a las poblaciones más vulnerables dentro de la estructura socioeconómica, limitando las posibilidades del desarrollo humano y, por ende, el desarrollo de una nación, debiendo ser abordada la problemática desde las políticas institucionales de educación superior en el marco de su Responsabilidad Social Universitaria –RSU– con la comunidad y el Estado. De ahí, la importancia del abordaje del tema de deserción UIS desde una mirada cuanti-cualitativa y en referencia a la RSU.

    18. Comparación de la actividad psíquica humana desde una perspectiva evolutiva

      Rosalía Montealegre

      1996-01-01

      Se parte de la teoría de la evolución de Darwin y se plantea: l. La acción de lo biológico y lo cultural en el desarrollo, analizada desde la psicología historicocultural, la etología humana, la sociobiología. Al centrarse este trabajo en la posición de la psicología historicocultural, se enfatiza lo cultural y el proceso de asimilación del desarrollo histórico de la sociedad por parte del individuo. 2. La actividad psíquica en primates, analizando lo psíquico desde la psicología ...

    19. Elementos del presupuesto del Seguro Popular en Chiapas desde el enfoque de género

      Magdalena del Carmen Morales Domínguez

      2011-01-01

      Full Text Available El objetivo de este trabajo es analizar elementos del presupuesto del Seguro Popular en Chiapas desde el enfoque de género. La metodología utilizada fue el método de Trazalíneas o Tracer para establecer rutas de seguimiento. Los resultados muestran que no existe un presupuesto constante para los programas de salud relacionados con género y que es un programa que responsabiliza a las mujeres de la afiliación por cada familia. En conclusión, el Seguro Popular trabaja bajo una lógica mercantilista que choca con el contexto pobre de la región, haciendo un uso enajenado del enfoque de género, sin tener una estrategia clara de cómo abordar la salud desde dicho enfoque.

    20. La pena de muerte desde la bioética y los derechos humanos

      Octavio Márquez Mendoza

      2015-01-01

      Full Text Available Se ofrece una reflexión de la pena de muerte, que parte de premisas esenciales desde la bioética y los derechos humanos. Se plantean algunas generalidades de esta disciplina, dado que orientan a un cuestionamiento respecto al aporte de la ciencia y la tecnología para llevar a cabo “ejecuciones más humanas”. Enseguida se recurre a los conceptos de vida, persona y dignidad para enmarcar el argumento en los derechos humanos. Se retoman algunas sentencias de documentos internacionales apelando a un desarrollo del hombre desde la ética: la práctica de la pena máxima, por el contrario, lo retiene. Así pues, se anticipa que lo humano nunca se hallará en la aniquilación de su propio género.

    1. 秦岭太白山北坡土壤拮抗性放线菌分布及特性%Distribution and characteristics of soil antagonistic actinomycetes on northern slope of Taibai Mountain, Qinling

      朱文杰; 薛泉宏; 曹艳茹; 薛磊; 申光辉; 来航线

      2011-01-01

      Twelve representative soil samples were collected from different altitudes on the northern slope of Taibai Mountain to study the distribution and characteristics of soil antagonistic actinomyces by using agar block method. There existed a great deal of soil antagonistic actinomyces in the study area. Among the 141 actinomycete strains isolated, 116 strains (82. 3% ) showed antagonism toward 12 target bacteria or fungi. The antagonistic strains at altitudes 800-1845, 3488, 3655, and 3670 m occupied 73.7% -86.8% , 81.3% , 78.9% and 82.3% of the total, respectively. 42. 1% of the strains at altitudes 1200-2300 m and >3400 m showed strong and broad spectrum antagonistic activity, suggesting that there was a great potential for the isolation of actinomycete strains with strong anti-biotic capability at these altitudes. 24. 1 % of the antagonistic actinomycetes showed antagonism against Staphyloccocus aureu, and 2.4% , 6.9% and 11. 2% of them showed activity toward Verticillium dahliae in cotton, Phytophthora sp. In strawberry and Neonectria radkiccla in ginseng, respectively. This study showed that the soil actinomycete antagonistic potentiality (SAAP) could be used as a quantitative indicator to evaluate the potential of antagonistic actinomycete resources in soil.%以太白山北坡12个不同海拔高度的代表性土样为研究对象,采用皿内琼脂块法筛选拮抗性放线茵.结果表明:在太白山北坡不同海拔高度土壤中生存着大量拮抗性放线菌,在分离出的141株放线菌中,有82.3%的放线菌对21种靶标菌有拮抗作用.其中,海拔800~1845、3488、3655和3670 m土壤中的拮抗性放线菌分别占其总数73.7%~86.8%、81.3%、78.9%及82.3%,在海拔1200~2300及3400 m以上土壤中分离到的广谱强拮抗性放线菌占其总数的42.1%.在拮抗性放线菌中,对金黄色葡萄球菌有强拮抗性的放线菌占拮抗菌总数的24.1%,对棉花黄萎病、草莓疫霉及人参

    2. Una aproximación a las representaciones de las bibliotecas en Andalucía desde los discursos de los usuarios potenciales

      Ruiz Ruiz, Jorge; Cerrillo Vidal, José Antonio

      2009-01-01

      En las últimas décadas se ha ido abriendo paso un nuevo modelo de entender las bibliotecas y la profesión de bibliotecario. En lugar de depósitos del saber, aislados del entorno, las bibliotecas se postulan actualmente como espacios de animación de la vida sociocultural de sus poblaciones, abiertas a múltiples usos por parte de públicos diferentes. Este artículo explora el lugar de las bibliotecas en el imaginario de la ciudadanía andaluza, basándose en el análisis de los discursos producidos...

    3. Necesidad de ecologizar la enseñanza de las Ciencias Naturales : una reflexión desde la Química

      Bonilla Cordero, M.; Di Moauro, A.

      2009-01-01

      La enseñanza de las Ciencias Naturales en las últimas décadas ha enfocado el desarrollo de sus contenidos de manera extremadamente específica aislados de una realidad y con poca vinculación entre el presente y futuro de una sociedad, creando áreas de conocimiento netamente especializadas sin ninguna relación entre ellas, ocasionando el poco desarrollo del aprendizaje significativo en el alumnado. El presente trabajo tiene como propósito, reflexionar sobre la necesidad de ecologizar la enseñan...

    4. CREACIÓN DE UNA UNIDAD DIDÁCTICA DESDE EL ENFOQUE DE LA MULTICULTURALIDAD. ALIMENTACIÓN Y NUTRICIÓN

      Antonio Becerra Hernández

      2009-01-01

      La multiculturalidad es un hecho en las sociedades modernas occidentales. Concretamente en nuestro país, lo que hace unos años eran casos aislados, a saber, la venida de personas pertenecientes a otras culturas para instalarse aquí con carácter definitivo, se ha convertido hoy en día en algo cotidiano. España, por otra parte, y debido a sus características propias en cuanto a lengua, cultura, historia y situación geográfica, recibe inmigrantes de muy diversas procedencias.

    5. Excursión desde Madrid a Valladolid el 20 de noviembre de 1955

      Valverde Gómez, José Antonio, 1926-2003

      2008-01-01

      Viaje desde Madrid a Valladolid el 20 de noviembre de 1955, del que se anotaron las especies de aves de cinco localidades, siendo en Villacastín (Segovia): Alauda arvensis (Alondra común), Corvus corone (Corneja negra), Falco tinnunculus (Cernícalo vulgar), Milvus milvus (Milano real), Pica pica (Urraca, llamada "marica" y "picarza" por el autor) y Sylvia undata (Curruca rabilarga), en Labajos (Segovia): Carduelis carduelis (Jilguero), Corvus corone (Corneja negra), Fringilla coelebs (Pinzón ...

    6. EL ROL SOCIAL DEL PROFESIONAL DE LA INFORMACIÓN: UN PUNTO DE VISTA DESDE ARGENTINA

      Julio Díaz Jatuf

      2015-06-01

      Full Text Available Este artículo propone abordar, cuestiones conceptuales sobre el valor de la información en el contexto de la Sociedad de la Información y el Conocimiento, relacionados a los paradigmas, competencias profesionales y la necesaria ética del ejercicio de la disciplina. Se profundiza sobre el rol social del profesional, desde experiencias tangibles latinoamericana y argentinas.

    7. Hablar desde el silencio: El silencio como verdad en las narrativas de mujeres sobrevivientes

      Karin Davidovich

      2015-01-01

      A partir del estudio de testimonios y entrevistas personales con ex detenidas-desaparecidas de la última dictadura militar en Argentina sostengo que, si bien todos los testimonios del horror están impregnados por la presencia del silencio, los testimonios de sobrevivientes argentinas que comienzan a surgir masivamente desde los  noventa contienen dos aspectos fundamentales que los distinguen de otros. El primero es que sugieren una diferencia importante entre dos tipos de silencio, uno mudo y...

    8. Eficacia de la infiltración de ozono paravertebral lumbar y en puntos gatillos como coadyuvante del tratamiento en pacientes con dolor lumbar crónico y lumbociatalgia crónica en el síndrome doloroso miofascial aislado o acompañado de otras patologías

      E. Silva Jiménez

      2014-02-01

      Full Text Available Introducción: Posterior al primer episodio de dolor lumbar, la recurrencia persiste durante un año o más en el 25 al 60 %, afectando a población económicamente activa, causando discapacidad y en 80 % ausentismo laboral. Objetivo: Evaluar el grado de eficacia del uso de la técnica de infiltración con ozono paravertebral lumbar y en puntos gatillos junto al tratamiento farmacológico y rehabilitador, en pacientes con dolor lumbar crónico y lumbociatalgia crónica debido al síndrome doloroso miofascial (SDM aislado o acompañado de otras patologías. Métodos: Estudio no probabilístico, de tipo experimental controlado, doble ciego. Se estudiaron 43 pacientes (22 experimental y 21 control. El grupo experimental recibió ozono paravertebral lumbar y en puntos gatillos más tratamiento farmacológico y rehabilitador. El grupo control recibió tratamiento farmacológico y rehabilitador. Se aplicó en ambos grupos la escala de EVA, Oswestry y la medición de los grados de flexión del tronco al inicio, dos y cuatro semanas posterior al comienzo del tratamiento. Resultados: La aplicación de ozono paravertebral lumbar y en puntos gatillos, junto al tratamiento farmacológico y rehabilitador, en el manejo de pacientes con dolor lumbar y lumbociatalgia crónica, comparado con solo tratamiento farmacológico y rehabilitador, resultó ser más eficaz, con significancia estadística (p < 0,05 para disminuir la intensidad del dolor (90,5 %, la incapacidad funcional (90,5 %, y aumentó los grados de flexión del tronco (85,7 % versus 40,0, 70 y 75 % respectivamente, a las cuatro semanas posteriores al inicio del tratamiento. Se evidencia mejoría en pacientes con SDM aislado o acompañado de síndrome de receso lateral, síndrome facetario, grados variables de hernia discal excepto la extrusión central. Conclusiones: La infiltración de ozono paravertebral lumbar y en puntos gatillos junto al tratamiento farmacológico y rehabilitador resultó ser m

    9. Abordando la problemática del Dengue desde una perspectiva ambiental

      Claudia Zuñiga Vega

      2009-01-01

      Full Text Available El Dengue es una enfermedad viral que genera danos cuantiosos en los países donde se desarrolla, afectando la economía, las actividades productivas y el turismo, entre otros. El principal transmisor de esta enfermedad es el mosquito Aedes aegypti y las campanas para erradicar este vector se han dirigido principalmente hacia la eliminación de sus larvas y adultos por medio de plaguicidas y hacia la reducción del mal manejo de los desechos. Sin embargo, no se ha realizado esfuerzos para determinar las razones por las que las poblaciones de este mosquito han sido tan exitosas, ni se ha aplicado enfoques integrales para luchar contra esta pandemia. Este artículo trata de explicar el incremento de los casos de Dengue desde la perspectiva del descontrol y desinterés ambiental y la falta de apoyo a las comunidades en muchos campos. Es por esto que los autores proponen abordar esta problemática desde una perspectiva ambiental integral, abarcando desde el control químico y biológico hasta la integración de todos los sectores involucrados (Gobierno, médicos y comunidad.

    10. Los dilemas europeos “2009” vistos desde Europa central y del este

      Aneta de la Mar Ikonómova

      2009-11-01

      Full Text Available En el presente artículo los dilemas europeos del año "2009" están enfocadas desde un ángulo poco común: desde Europa Central y Europa del Este. La premisa es que este enfoque no tradicional permite una mayor comprensión del continente europeo, entendido como una gran región compleja, diversa y unida bajo la dinámica de constantes acercamientos y distanciamientos de los intereses particulares de cada país, sea miembro o no de la Unión. Es evidente que la Unión Europea es un logro para todo el continente, sin embargo, quedan preguntas y asuntos no resueltos, además, unos de éstos se refieren al futuro de la ampliación de la Unión y de la OTAN, y parece que no siempre esta problemática se puede entender suficientemente bien si se mira y analiza exclusivamente desde Bruselas.

    11. El clima organizacional en universidades de Bogotá desde la perspectiva de los estudiantes

      José Javier Bermudez-Aponte

      2015-08-01

      Full Text Available Esta investigación planteó como objetivo caracterizar el clima organizacional de seis universidades, localizadas en la ciudad de Bogotá Distrito Capital (D.C., Colombia, a partir de las percepciones expresadas por algunos de sus estudiantes. La metodología se abordó desde el enfoque cualitativo, con alcance descriptivo, busca comprender el fenómeno objeto de análisis desde la visión de conjunto, las posibilidades de análisis de lo subyacente y el sentido de construcción del objeto. Los hallazgos fueron agrupados en cuatro categorías: académica, socio- afectiva, administrativa y ética. Los resultados evidenciaron, desde la percepción de los estudiantes, que el clima organizacional universitario es el conjunto de factores tangibles (infraestructura, recursos, etc. e intangibles (valores, relaciones interpersonales, situaciones, etc., que se viven a diario en la universidad. La dinámica entre estos factores es bidireccional, es decir, cada uno de ellos puede ser actor u objeto de afectación en relación con otro.

    12. Repensando la etnicidad y el transnacionalismo desde el análisis de redes personales

      Javier Augusto Ávila Molero

      2015-12-01

      Full Text Available La etnicidad y el transnacionalismo se ha entendido desde los vínculos que los migrantes mantienen con personas de su sociedad y cultura de origen, pero no desde una vision “integral” de sus vínculos, que incluya también a personas de la sociedad de destino o de otros orígenes culturales. El artículo presenta una propuesta para “medir” la etnicidad y el transnacionalismo en los migrantes desde el análisis de las propiedades de composición de sus redes personales. Los resultados del estudio se elaboraron con una muestra de 293 redes de los siguientes colectivos migrantes: argentinos, dominicanos, peruanos, marroquíes y senegaleses en Barcelona, España. Los datos muestran que las redes de los migrantes tienen una estructura transnacional “multisituada” no solo entre los países de origen y destino sino también otros terceros y una composicón multiétnica. Los datos muestran también que entre los migrantes recientes los niveles de transnacionalismo son mayores y entre los migrantes más antiguos los niveles de transnacionalismo son mayores.

    13. Screening of Rhizospheric Actinomycetes for Various In-vitro and In-vivo Plant Growth Promoting (PGP) Traits and for Agroactive Compounds.

      Anwar, Sumaira; Ali, Basharat; Sajid, Imran

      2016-01-01

      In this study 98 rhizospheric actinomycetes were isolated from different wheat and tomato fields, Punjab, Pakistan. The isolates were characterized morphologically, biochemically, and genetically and were subjected to a comprehensive in vitro screening for various plant growth promoting (PGP) traits. About 30% of the isolates screened were found to be the promising PGP rhizobacteria (PGPRs), which exhibited maximum genetic similarity (up to 98-99%) with different species of the genus Streptomyces by using16S rRNA gene sequencing. The most active indole acetic acid (IAA) producer Streptomyces nobilis WA-3, Streptomyces Kunmingenesis WC-3, and Streptomyces enissocaesilis TA-3 produce 79.5, 79.23, and 69.26 μg/ml IAA respectively at 500 μg/ml L-tryptophan. The highest concentration of soluble phosphate was produced by Streptomyces sp. WA-1 (72.13 mg/100 ml) and S. djakartensis TB-4 (70.36 mg/100 ml). All rhizobacterial isolates were positive for siderophore, ammonia, and hydrogen cyanide production. Strain S. mutabilis WD-3 showed highest concentration of ACC-deaminase (1.9 mmol /l). For in-vivo screening, seed germination, and plant growth experiment were conducted by inoculating wheat (Triticum aestivum) seeds with the six selected isolates. Significant increases in shoot length was observed with S. nobilis WA-3 (65%), increased root length was recorded in case of S. nobilis WA-3 (81%) as compared to water treated control plants. Maximum increases in plant fresh weight were recorded with S. nobilis WA-3 (84%), increased plant dry weight was recorded in case of S. nobilis WA-3 (85%) as compared to water treated control plants. In case of number of leaves, significant increase was recorded with S. nobilis WA-3 (27%) and significant increase in case of number of roots were recorded in case of strain S. nobilis WA-3 (30%) as compared to control plants. Over all the study revealed that these rhizospheric PGP Streptomyces are good candidates to be developed as

    14. Metabolomics of the Bio-Degradation Process of Aflatoxin B1 by Actinomycetes at an Initial pH of 6.0

      Manal Eshelli

      2015-02-01

      Full Text Available Contamination of food and feed by Aflatoxin B1 (AFB1 is a cause of serious economic and health problems. Different processes have been used to degrade AFB1. In this study, biological degradation of AFB1 was carried out using three Actinomycete species, Rhodococcus erythropolis ATCC 4277, Streptomyces lividans TK 24, and S. aureofaciens ATCC 10762, in liquid cultures. Biodegradation of AFB1 was optimised under a range of temperatures from 25 to 40 °C and pH values of 4.0 to 8.0. An initial concentration of 20 µg/mL of AFB1 was used in this study. The amount of AFB1 remaining was measured against time by thin layer chromatography (TLC and high-performance liquid chromatography (HPLC, coupled with UV and mass spectrometry (LC-MS. All species were able to degrade the AFB1, and no significant difference was found between them. AFB1 remained in the liquid culture for R. erythropolis, S. lividans and S. aureofaciens were 0.81 µg/mL, 2.41 µg/mL and 2.78 µg/mL respectively, at the end of the first 24 h. Degradation occurred at all incubation temperatures and the pH with the optimal conditions for R. erythropolis was achieved at 30 °C and pH 6, whereas for S. lividans and S. aureofaciens the optimum conditions for degradation were 30 °C and pH 5. Analysis of the degradative route indicated that each microorganism has a different way of degrading AFB1. The metabolites produced by R. erythropolis were significantly different from the other two microorganisms. Products of degradation were identified through metabolomic studies by utilizing high-resolution mass spectral data. Mass spectrometric analysis indicated that the degradation of AFB1 was associated with the appearance of a range of lower molecular weight compounds. The pathway of degradation or chemical alteration of AFB1 was followed by means of high resolution Fourier transform mass spectrometry (HR-FTMS analysis as well as through the MS2 fragmentation to unravel the degradative pathway for

    15. 放线菌剂与腐植酸钾对魔芋抗病促生效果研究%Study of Combined Application of Actinomycetes Biocontrol Agents and Potassium Humate on Disease Resistance and Growth-promoting Effect of Amorphophallus Konjac

      张忠良; 刘列平; 何斐

      2014-01-01

      分别以不施菌剂、不施腐植酸钾为对照,采用小区试验,探讨生防放线菌剂与腐植酸钾配施条件下二者对魔芋的防病促生作用。试验结果表明:(1)​当腐植酸钾与3号放线菌配施时,具有较强的防病促生作用。30克/株、60克/株、90克/株腐植酸钾分别配施3号菌剂时,与不施腐植酸钾对照相比,魔芋病害相对防效、增产率及增长系数分别提高了9.8%~41.6%、17.1%~76.3%和20.1%~64.2%。其中,60克/株腐植酸钾配施3号菌剂时效果最明显。(2)​当放线菌剂与腐植酸钾配施时,具有较强的防病促生作用。1、2、3号放线菌剂与60克/株腐植酸钾配施,与不施菌剂对照相比,魔芋病害相对防效、增产率及增长系数分别提高了11.7%~89.7%、33.3%~150.9%和38.5%~175.0%。其中2、3号放线菌剂与60克/株腐植酸钾配施处理效果较好。研究表明,腐植酸钾与放线菌剂配施能促进魔芋生长,提高魔芋产量,降低发病率。%With no actinomycetes and potassium humate treatments as control(CK), the effect of actinomycetes and potassium humate on disease resistance and growth-promoting of Amorphophallus konjac in the plot trials were evalu-ated. Results showed that:(1) Combined application of potassium humate and actinomycetes No.3 had strong disease resistance and growth-promoting effect. Under 30, 60, 90 gram per plant of potassium humate combined with actinomy-cetes No.3 treatment, biocontrol effect increased by 9.8%~41.6%, yield increased by 17.1%~76.3%and growth factor increased by 20.1%~64.2% compared with the group without potassium humate. Among which, the combined ap-plication of 60 gram per plant of potassium humate and actinomycetes No.3 had the most obvious effect. (2) Combined application of actinomycetes and potassium humate had signiifcant anti-disease and growth-promoting effect. Three kind of actinomycetes combined with 60 gram per plant of potassium

    16. El sistema de la educación en Luhmann desde una perspectiva crítica

      Carlos Massé

      2007-01-01

      El trabajo confronta los elementos de los que parte Luhmann para teorizar los sistemas educativos, desde una forma crítica de centrar el enfoque idóneo para conocer la multiplicidad de elementos que permean los sistemas educativos. Se critican los fundamentos de que Luhmann parte y se proponen otros niveles de problematización, desde una visión crítica de las ciencias sociales.

    17. 青岛泊子盐场放线菌多样性及其功能酶的筛选%Biodiversity of actinomycetes from Pozi saltern in Qingdao and the screening for functional enzymes

      常显波; 刘文正; 张晓华

      2015-01-01

      In this study ,15 actinomycetes were isolated using three different media (M1~M3 ) from a salt pond of Qingdao .Bioinformatics analysis of the 16S rRNA sequences from the isolates revealed they belonged to three genera (Streptomyces ,Nocardiopsis and a novel genus) ,with Streptomyces being the dominant genus .The strain CXB832 were 95 .4 % and 94 .9 % homologous to the two standard strains of bacteria ,and therefore was candidates for a new genus on the basis of phenotypic ,chemotaxonomic and phylogenetic distinctiveness .The type of medium and temperature showed significant effects on the number and diversity of actinomycetes recovered .Starch casein medium was the most effective media to isolate actinomycetes species at 37℃ .12 ,3 ,2 and 1 of 15 isolates produces amylase ,lipase ,caseinase and cellulose ,respectively .%采用平板涂布法利用3种培养基从青岛即墨市田横镇泊子盐场盐池底泥样中共分离纯化出15株放线菌,对分离菌株进行16S rRNA基因测序分析。发现这15株放线菌分属于链霉菌属( Streptomyces )、拟诺卡氏菌属(Nocardiopsis)和1个新属,其中 Streptomyces属为优势菌属;菌株CXB832与 Nocardiopsis arabia DSM 45083T和 Haloacti-nospora albus DSM 45015T最接近,同源性分别为95.4%和94.9%,根据其表型和分子特征可以初步判定该菌株为放线菌新属。培养基及培养温度对盐池环境中放线菌的分离效果均有影响,利用淀粉酪素培养基在37℃分离的放线菌种类和数量较多。在15株放线菌中,分别有12株、3株、2株和1株放线菌产淀粉酶、酯酶、蛋白酶和纤维素酶。

    18. APORTES A LA PROBLEMÁTICA CIENCIA-ESPIRITUALIDAD DESDE EL BUDISMO ZEN

      José Arlés Gómez Arévalo

      2005-01-01

      Full Text Available Como fruto de la reflexión entorno al tema de "ciencia y espiritualidad", se ha venido indagando, en este trabajo investigativo, por las conexiones que se pueden establecer entre el mundo de la ciencia occidental y la espiritualidad oriental. Este trabajo ha sido elaborado desde los presupuestos de la física cuántica y la teoría de la complejidad, en contraste con las antiguas tradiciones espirituales del lejano Oriente, concretamente, por el aporte del budismo zen, ya que desde sus fundamentos teórico-prácticos proporciona elementos básicos en la comprensión de las problemáticas del hombre contemporáneo a nivel del conocimiento, el arte, la ciencia y la espiritualidad. Las eternas preguntas, que han sido explicadas por las antiguas tradiciones espirituales y otras veces por las filosofías convencionales, hoy por hoy conducidas por la mano de científicos altamente reconocidos por la comunidad científica occidental, arrojan resultados tales que pareciera ser que la brecha entre ciencia y espiritualidad, que comenzó desde tiempos remotos, se está cuestionando a la luz de las nuevas teorías de la física cuántica, la teoría sistémica, la psiconeuroinmunología, la neuroteología y muchas otras derivadas de las reflexiones, los experimentos y las tecnologías que se han implementado en los últimos tiempos y que pretenden dar respuesta a los problemas ya planteados.

    19. Fatty acid biosynthesis in actinomycetes

      Gago, Gabriela; Diacovich, Lautaro; Arabolaza, Ana; Tsai, Shiou-Chuan; Gramajo, Hugo

      2011-01-01

      All organisms that produce fatty acids do so via a repeated cycle of reactions. In mammals and other animals, these reactions are catalyzed by a type I fatty acid synthase (FAS), a large multifunctional protein to which the growing chain is covalently attached. In contrast, most bacteria (and plants) contain a type II system in which each reaction is catalyzed by a discrete protein. The pathway of fatty acid biosynthesis in Escherichia coli is well established and has provided a foundation fo...

    20. Cambio climático y salud humana: un mensaje reiterado desde 1995

      Eduardo Calvo

      2008-10-01

      Full Text Available En agosto pasado, se celebró el XX aniversario del IPCC (Panel Intergubernamental sobre Cambio Climático. Desde el segundo informe de evaluación, el IPCC viene enfatizando sobre los potenciales efectos del clima sobre la salud. Sin embargo, alrededor del mundo, las enfermedades relacionadas con el clima, más allá de las victimas de los llamados desastres naturales, vienen aumentando en todas las latitudes. Este trabajo presenta un breve panorama sobre los informes y el panorama general con el que se relaciona.

    1. Las NTI en la educacion a distancia desde el aula virtual veterinaria

      Soler Pellicer, Yolanda; Rodríguez Valera, Yoel; Antúnez Sánchez, Guillermo; Ramírez Sánchez, Waldo; Linares Alvaro, Manuel

      2005-01-01

      La Educación a Distancia, abre campos insospechados en el desarrollo de la Medicina Veterinaria actual y futura, potenciando un despertar de los profesionales veterinarios. Una herramienta que ha aprovechado las nuevas tecnologías, es la multimedia que utiliza las posibilidades de video y audio en general, imágenes y textos. Esta influencia se extiende a las investigaciones Desde veterinaria.org se desarrollan cursos a través de la plataforma del aula virtual veterinaria www.cursosonline.net ...

    2. Guerra Fría: lecturas desde la izquierda sobre un mundo bipolar

      Zurita, María Delicia

      2007-01-01

      El fin de la Segunda Guerra Mundial configuró un nuevo orden mundial que reformuló el campo de las Relaciones Internacionales, produciéndose el paso de un mundo multipolar a un mundo bipolar. Los protagonistas de este nuevo escenario eran dos superpotencias que se definían por sus notables diferencias desde los puntos de vista político, económico e ideológico: Estados Unidos y la Unión Soviética. El constante enfrentamiento entre ambas convirtió al mundo en un espacio de posibles batallas fut...

    3. Acercamiento a la educación holística desde una perspectiva actual

      Iturri Urtasun, Cristina

      2013-01-01

      La educación holística tiene como objetivo formar al ser humano de manera integral, en todos sus aspectos, físico, biológico, espiritual, intelectual y emocional. Desde la época clásica hasta la actualidad se ha visto la necesidad de educar de manera integral al ser humano, pero hasta hace una década, no se le da el nombre de educación holística tal y como la entendemos actualmente. En el contexto europeo, a pesar de no haber tenido gran impacto a nivel de práctica educativa, se aprecia que t...

    4. Caracterizando a la natación desde el enfoque praxiológico

      Domínguez, María Eugenia; Saraví, Jorge Ricardo

      2013-01-01

      En esta ponencia llevaremos adelante una caracterización de la natación desde la praxiología motriz, teniendo en cuenta variables, definiciones, clasificaciones de diferentes autores. Parlebas (2001) incluye a la natación dentro de los deportes psicomotrices. La natación es una práctica psicomotriz porque no se llevan a cabo interacciones motrices esenciales con otros, es decir, tiene que ver con actividades motrices realizadas en solitario, sin intercambio operatorio con los demás. A partir ...

    5. Nuevos retos para combatir la violencia de género desde el sistema educativo

      Vázquez Verdera, Victoria

      2013-01-01

      El presente artículo tiene por objeto repensar las finalidades del sistema educativo a la luz del nuevo marco de interpretación de la violencia contra las mujeres que impulsó la Ley orgánica 1/2004 de 28 de diciembre, de Medidas de Protección Integral contra la Violencia de Género y de la investigación feminista en este ámbito. Consecuencia de la reflexión pedagógica realizada, se identifican nuevos retos para prevenir la violencia de género desde el ámbito educativo. Se conclu...

    6. Movilidad cotidiana y sostenibilidad, una interpretación desde la Geografía Humana

      Miralles-Guasch, Carme, 1961-; Cebollada Frontera, Àngel

      2009-01-01

      La movilidad cotidiana es un fenómeno creciente y complejo, integrado por diferentes dimensiones, que en los últimos tiempos esta siendo objeto de una revisión metodológica y conceptual, y la Geografía Humana no ha sido ajena a ello. El artículo ahonda en esta transformación desde tres perspectivas complementarias, los cambios en el modelo económicoterritorial,la introducción del paradigma de la sostenibilidad y la inclusión de la estructura social de la población móvil. Tres perspectivas que...

    7. La estiba y desestiba portuaria. Un estudio desde el Derecho Administrativo.

      Menéndez de la Cruz, Cristina

      2015-01-01

      La estiba y desestiba en el entorno portuario no ha sido analizada desde la óptica del Derecho Administrativo, por lo que a mi juicio era pertinente hacer un estudio dada la significación que el fenómeno tiene para el conjunto de la economía. No ha ocurrido lo mismo en otras disciplinas jurídicas, como el Derecho Laboral y el Mercantil, pudiendo encontrar numerosos estudios sobre esta materia. Esto es debido al carácter de zona fronteriza del Derecho que adoptan algunos temas, como apunta el ...

    8. Psicología y discapacidad: un encuentro desde el paradigma social

      Lucrecia Alfaro-Rojas

      2013-01-01

      El tema de la discapacidad ha sido abordado desde diversos paradigmas o modelos que han dictado el modo de brindar atención a personas con esta condición. Aunque en la literatura se encuentran diversas denominaciones para estos paradigmas, el presente artículo se refiere a los tres paradigmas que engloban las posturas más habituales hacia el tema de la discapacidad: el paradigma tradicional, el paradigma médico-biológico y el paradigma social. Es dentro de este último que se concibe a la p...

    9. La organización del conocimiento desde la perspectiva poscolonial. Itinerarios de la paraconsistencia

      García Gutiérrez, Antonio Luis

      2013-01-01

      Los procesos de producción, transmisión y organización del conocimiento son interferidos, como el resto de las prácticas simbólicas, por acciones colonizantes culturales y cognitivas desde los inicios de su sistematización. La desclasificación propone la rehabilitación de viejos valores desterrados por el pensamiento hegemónico como la estesia, la incertidumbre, la provisionalidad, la retórica o la contradicción con el fin de obtener una organización del conocimiento e...

    10. Subjetividad, cotidianidad y narrativa. Apuntes para pensar la subjetividad desde una mirada situada

      Lady Paola Rojas

      2014-01-01

      Pensar la subjetividad desde una mirada situada es una tarea que sobrepasa los límites del pensamiento científico y obliga a repensar sus configuraciones a la luz del mundo de la vida cotidiana. En este ar - tículo se realiza una reflexión sobre las posibilida - des de realizar este tránsito; para esto, en un primer momento se hace un recorrido por algunas de las transformaciones epistemológicas que ha tenido el abordaje de la subjetividad en la psicología, ha - ciendo énfasis en la psi...

    11. Espacio y subjetividad. Orden social desde lo privado y lo público

      Graciela Vélez Bautista

      2005-01-01

      En este artículo se plantea la relación entre subjetividad, espacio y género como uno de los enfoques que explican la identificación de los hombres con el espacio público y de las mujeres con el espacio privado, desde una perspectiva cultural e histórica, cuya huella en la subjetividad prevalece a pesar de las transformaciones modernas en la concepción de lo femenino y masculino, así como de lo privado y público.

    12. Marcaciones para y desde la formación de los arquitectos

      Seguí de la Riva, Javier

      2008-01-01

      Dedicarse a la formación de arquitectos supone una radicalización en el entendimiento de la arquitectura y el urbanismo, que no tiene porque patentizarse de igual modo en el ejercicio de la profesión. Nosotros, desde nuestro grupo de investigación (Hypermedia: Taller de configuración arquitectónica) hace tiempo que observamos los debates públicos entorno a la edificación, la arquitectura y el urbanismo y asistimos a las problemáticas en el aprendizaje y la teorizaron del proyectar, reflexion...

    13. Abordaje de un caso clínico tratado desde una perspectiva integradora

      Montes-Berges, Beatriz; Moreno Ratia, Mª Victoria

      2011-01-01

      Este trabajo tiene como objetivo presentar la eficacia de una terapia de integración. Esto supone ir más allá de los límites de los enfoques de escuelas únicas, integrando técnicas y descubrimientos que aunque en principio pueden parecer, en determinadas ocasiones, incompatibles a nivel teórico, resultan enriquecedores en la práctica clínica. Desde esta perspectiva, se ha llevado a cabo el tratamiento de una usuaria adscrita al programa de Violencia de Género, dada la gran vari...

    14. La evaluación de la docencia en lenguaje musical desde una perspectiva integradora

      Esteve-Faubel, José-María; Molina Valero, Miguel Ángel; Botella Quirant, María Teresa; Espinosa Zaragoza, Juan Antonio; Esteve Faubel, Rosa Pilar

      2010-01-01

      Póster y comunicación presentados en las VIII Jornadas de Redes de Investigación en Docencia Universitaria "Nuevas titulaciones y cambio universitario", Alicante, 8-9 Julio 2010. El presente estudio tiene como objetivo evaluar la docencia en la asignatura de Lenguaje Musical, titulación de Magisterio especialidad Musical, desde una perspectiva amplia y combinando tanto el método cualitativo como cuantitativo como medio para conocer con la mayor profundidad posible el tema tratado. Dada la ...

    15. Repensar lo estratégico desde la comunicación

      Echeverría Ramírez, María Clara

      2002-01-01

      En este ensayo se sitúa la mirada en la comunicación como entrada para re-pensar la democratización de la planeación. Se introduce como problema enunciado, la necesidad de comunicar lo antrópico, lo biótico y lo físico. Para abordar la comunicación y la planeación se proponen criterios sobre asuntos como: orden, homogeneidad, democracia, participación, escalas, noción de desarrollo, hegemonía, estética y temporalidad. Se concluye con una propuesta que contiene elementos conceptuales desde la ...

    16. Directrices comunicacionales desde la ISO 26000, para la empresa socialmente responsable

      Orjuela Córdoba, Sandra

      2012-01-01

      La gestión de Responsabilidad Social Corporativa requiere de la comunicación y a su vez la comunicación debe ser responsable, ya que la organización se relaciona con su entorno, construyendo cultura desde su identidad y proyectándola a través de los comportamientos responsables con sus audiencias. La ISO 26000, incluye el tema de la comunicación, demostrando su importancia para el éxito de la RSC. El enfoque táctico de la ISO, se complementa con la propuesta de expertos en Relaciones Púb...

    17. Jóvenes Latinos en Barcelona. Un relato desde el espacio escolar.

      Palou Diez, Montse

      2007-01-01

      La investigación Espacio público y nuevas formas de sociabilidad. Jóvenes de origen latinoamericano en Barcelona (2005), dirigida por el antropólogo Carles Feixa realizada con el acompañamiento de un equipo de trabajo multidisciplinar, en el cual participe supuso un ejercicio de antropología implicada en la línea abierta por el Dr. Oriol Romaní, será el fundamento de esta reflexión desde el ámbito educativo. Apartir de ella nos adentraremos en una lectura posible de los procesos migratorios d...

    18. Análisis de la Incapacidad Temporal desde la Atención Primaria

      Eugenio Fernández Escalada

      2014-01-01

      La IT es una de las principales prestaciones del Sistema Nacional de la Seguridad Social. En el control y gestión de dicha prestación estamos implicados muchos profesionales de distintas entidades: Atención Primaria, Atención Especializada, Inspección Médica, INSS, Mutuas, etc. No debemos obcecarnos en ver solo los problemas que nos genera la gestión de la IT desde nuestro punto de vista. Si realmente queremos que la gestión de la IT sea lo más eficiente posible, la colaboración entre todos e...

    19. Con, por, en, desde y para la comunidad. Cartas al Director.

      Esteban Coto Ezama

      1986-01-01

      Full Text Available El Movimiento Comunitario en Salud Mental es un movimiento distributivamente justo y humanizador, pero sólo dentro de los límites que permite la metáfora de la enfermedad. Estos límites se han encarnado en una antigua práctica: tomar partido en los conflictos negándolo. Obligado a responder a las demandas sociales, el movimiento sobrevive o malvive asumiendo las funciones desde siempre encomendadas a Hospitales Psiquiátricos y Manicomios. Y es que no es esperable que sobrevivan nuevas posturas morales sin la asunción de nuevas creencias

    20. El cine desde la perspectiva de la ciencia pol??tica

      Trenzado Romero, Manuel

      2000-01-01

      La cultura de masas es probablemente uno de los fen??menos peculiares m??s importantes del siglo XX que menos atenci??n han recibido desde la perspectiva de la Ciencia Pol??tica. En este art??culo indagamos en primer lugar en los motivos que han podido motivar este desinter??s politol??gico por la cultura de masas en general y, en particular, por el cine, uno de sus m??s claros exponentes. En el ep??grafe segundo damos cuenta de la manera en que la disciplina de la Comunicaci??n Pol??tica, ye...

    1. Cambio climático y salud humana: un mensaje reiterado desde 1995

      Eduardo Calvo

      2008-01-01

      En agosto pasado, se celebró el XX aniversario del IPCC (Panel Intergubernamental sobre Cambio Climático). Desde el segundo informe de evaluación, el IPCC viene enfatizando sobre los potenciales efectos del clima sobre la salud. Sin embargo, alrededor del mundo, las enfermedades relacionadas con el clima, más allá de las victimas de los llamados desastres naturales, vienen aumentando en todas las latitudes. Este trabajo presenta un breve panorama sobre los informes y el panorama general con e...

    2. La violencia escolar en Colombia desde la perspectiva sistémica de Talcott Parsons

      Palomino-Leiva, Martha Liliana

      2015-01-01

      El eje central de esta investigación es estudiar el fenómeno de la violencia escolar a partir de la teoría sociológica de Talcott Parsons. Desde esta perspectiva la violencia escolar se entiende como una conducta desviada de los escolares motivada por actitudes, valores, normas sociales, creencias y actos simbólicos, manifiestos en su vida cotidiana. Estudio cuantitativo de tipo descriptivo realizado sobre una fuente secundaria, una encuesta oficial, aplicada en la ciudad de Bo...

    3. Resignificando la realidad desde sus pliegues: los oficios de Rafael Baena

      Carlos Andrés Almeyda Gómez

      2014-01-01

      Hacia 1895, Miguel de Unamuno introdujo en la discusión sobre la historia y el sentir de España, el concepto de intrahistoria como la posibilidad dialéctica de romper con el cariz totalizante y esquemático de la historicidad a través del agregado que supone la confrontación constante entre dicha abstracción de la realidad y el flujo continuo e imperecedero de lo intrahistórico, esto es, la recuperación, acaso la reformulación de ese pasado, desde la lengua y el parecer popular, reabriendo aqu...

    4. La medicina complementaria y alternativa desde el punto de vista del traductor

      Cristina Estrada Velo

      2011-01-01

      La traducción de textos de medicina complementaria y alternativa (MCA) esconde dificultades inesperadas para el traductor. Este se debe a que la MCA engloba un amplio y variado grupo de sistemas de diagnóstico y tratamiento y a que además se basa en conceptos, filosofías y técnicas muy distintas a las de la medicina occidental actual que todos conocemos (también denominada medicina convencional). Desde principios de los años setenta la medicina tradicional china (MTC) se está expandiendo con...

    5. La interdisciplinariedad en los contenidos de Secundaria Básica desde las ciencias naturales

      Daniel Jesús Chacón-Rodríguez; Fara Estrada-Sifontes; Guadalupe Moreno-Toirán

      2013-01-01

      Aborda una propuesta de contenidos para la interdisciplinariedad que debe dominar un egresado de Secundaria Básica, desde las ciencias naturales, para alcanzar la formación de un pensamiento con visión holística de la realidad. Considera las necesidades sociales de la realidad inmediata del escolar, se relaciona con los avances de la ciencia y la técnica y prevé que el escolar pueda influir positivamente en su solución. Contribuirá a prepararlos para enfrentar problemas de la vida cotidiana y...

    6. ¿QUÉ ES EL DINERO? UN ABORDAJE DESDE LA PSICOLOGÍA ECONÓMICA

      Gustavo Guzmán Fernández

      2000-01-01

      Este artículo se desenvuelve alrededor de cuatro líneas argumentales en relación al dinero. Por un lado, parte de una breve introducción que toma en cuenta tanto su origen, como hijo legítimo del comercio, como la influencia que ha tenido en la expansión de antiguos imperios. En la segunda sección se presentan aspectos históricos y contextuales que han llevado a materializarlo de diferentes formas en los distintos núcleos humanos que abarcan desde pieles hasta conchas de cau...

    7. Competencias distintivas en las pymes: un aporte desde gestión humana

      Gregorio Calderón Hernández

      2006-01-01

      Con el fin de establecer de qué manera se está apoyando la competitividad por parte de la gestión humana en las pymes, se adelantó una investigación en 16 pequeñas y medianas unidades productivas de Manizales, en donde se estudiaron las competencias distintivas que más inciden sobre los factores de competitividad empresarial. Desde el punto de vista teórico se tomaron el enfoque sistémico de la competitividad, la teoría de recursos y capacidades, y la perspectiva estratégica de la gestión hum...

    8. Las limitaciones del «modelo Barcelona». Una lectura desde Urban Regime Analysis

      Casellas, Antònia

      2006-01-01

      El artículo explora el modelo de desarrollo urbano en Barcelona, utilizando Urban Regime Analysis como herramienta analítica. Se presentan las principales características de gobernabilidad de la ciudad y su evolución desde principios de la década de 1980 hasta el año 2006. Se analiza la dinámica de la transformación de la ciudad estudiando con detalle el proceso de consolidación de la colaboración entre los sectores público y privado iniciada en el contexto de los Juegos Olímpicos de 1992. Se...

    9. EMPRESARIALIDAD Y EMPRESA: UNA APROXIMACIÓN DESDE LA ESCUELA AUSTRIACA

      GEOVANNY PERDOMO CHARRY

      2010-01-01

      Este artículo de revisión teórica de las categorías Empresarialidad y Empresa es abordado desde la escuela austriaca, debido a su alta influencia en los estudios de administración en la sociedad capitalista actual. Se hace una descripción de la empresarialidad, empresario, emprendedor y empresa con el propósito de argumentar posibles trabajos de investigación en administración, que soporten teóricamente la creación de empresas y el fortalecimiento empresarial a nivel mundial; haciendo hinc...

    10. Falacias del desarrollo sustentable: una crítica desde la metamorfosis conceptual

      Daniel Murillo Licea

      2004-01-01

      Se revisan los orígenes de los conceptos de desarrollo rural y desarrollo sustentable desde un punto de vista en el que las ciencias del lenguaje, la semiótica y la comunicación entran en juego. Se presentan, a grandes rasgos, algunos elementos que aparecen en la determinación del uso y empleo de programas aplicados en México, así como dos ejemplos donde el desarrollo rural fue un pretexto para la aplicación y la actuación de algunas instituciones públicas.

    11. La generalización de patrones desde una perspectiva semiótico-cultural

      Gómez, John; Vergel, Rodolfo

      2012-01-01

      En esta ponencia se presenta un avance de la tesis de maestría “El pensamiento algebraico desde una perspectiva semiótico - cultural. Un trabajo con sucesiones de números reales” que se viene desarrollando en la Maestría en Docencia de las Matemáticas de la Universidad Pedagógica Nacional. Se presenta el análisis hecho a uno de los grupos objeto de la investigación utilizando como marco de referencia el enfoque semiótico - cultural propuesto por Radford (2008, 2009, 2010) sobre el pensamiento...

    12. La lucha contra el maltrato infantil desde el ámbito sanitario

      Carmena Hernández, Ana Isabel; Vidal Salcedo, Ana; Jiménez Carabias, María del Rocío

      2015-01-01

      Cartel presentado en la Segunda Conferencia Internacional de Comunicación en Salud, celebrada el 23 de octubre de 2015 en la Universidad Carlos III de Madrid Introducción: el maltrato infantil ha sido, y es, una preocupación mundial expresada en multitud de asambleas generales de naciones unidas, con el fin de proteger los derechos de los niños. Desde principios del siglo XX, en España se lleva generando una ley que proteja a la infancia, que fomente unos buenos cuidados y una crianza salu...

    13. Protocolo para el Abordaje Integral de la Violencia Sexual desde el Sector Salud

      Cuevas Garavito, Aura; Pinilla Moya, Fernando; Barrios Acosta, Miguel; Gómez Sánchez, Pio Iván; Vásquez Rojas, Rafael; Cárdenas Rivera, Miguel Eduardo; Pardo Sierra, Fideligno; Urrego Mendoza, Zulma Consuelo; Ángel Muller, Edith; Cuervo Maldonado, Sonia; Macana Tuta, Neidy; Espitia Cruz, Martha Isabel; Torres, Petty Janeth; Botia, Luis; Hernández Molina, Luz Mery

      2008-01-01

      La Secretaría Distrital de Salud, como institución responsable de ejecutar políticas de salud pública, está comprometida con la promoción del buen trato y la prevención y atención de la violencia sexual. Cumpliendo con sus funciones dentro del actual Sistema General de Seguridad Social en Salud, la Secretaría Distrital de Salud ha creado el presente documento, Protocolo para el abordaje integral de la violencia sexual desde el Sector Salud, para brindar herramientas a las instituciones públic...

    14. Procesos de significación mediados por una plataforma de aprendizaje colaborativo desde los protagonistas

      Marcelo Arancibia Herrera; Iván Oliva Figueroa; Francisco Paiva Cornejo

      2014-01-01

      El uso de tecnologías de la comunicación (TIC) en el aula escolar requiere la creación de propuestas contextualizadas que fomenten la colaboración y el uso de recursos cercanos a los estudiantes. El presente artículo muestra los resultados asociados a los procesos de significación, que profesores y estudiantes construyeron desde su participación en un proyecto en el que se implementaron diseños didácticos colaborativos con uso de TIC, en particular de redes sociales. Se efectuaron grupos foca...

    15. La vida cotidiana en Siete Aguas desde Cavanilles al Gran Fondo (1795-1985)

      Agulló Albuixech, Recaredo; Agulló Calatayud, Víctor; Fuertes Banacloig, Federico

      2014-01-01

      La vida cotidiana en Siete Aguas desde 1795 hasta 1985 es un inmenso escaparate social en el que quedan reflejados los quehaceres de una pequeña población del interior de la provincia de Valencia. La metodología utilizada se centra en el análisis de las noticias que la prensa valenciana y española dedicaron a este pueblo de apenas 1200 habitantes situado a más de 700 metros de altitud. La obra se estructura en una veintena de capítulos temáticos específicos o generales en los que existe una o...

    16. Los sitios de Zaragoza. Rutas urbanas para educar desde la ciudad.

      Huerta, Ricard

      2014-01-01

      El presente trabajo revisa el esfuerzo que se ha llevado a cabo desde la municipalidad de Zaragoza para impulsar un proyecto de educación patrimonial que pone en valor tanto los restos arqueológicos hallados hace unos años (Teatro Romano, Termas Públicas) como otros espacios urbanos musealizados (Museo del Foro, Museo del Puerto Fluvial) en base a un recorrido urbano que culmina en el espléndido Museo Pablo Gargallo. De esta propuesta nos interesa tanto la vertiente referida a la educación en...

    17. Desde Tláhuac hacia la sociedad que queremos: notas para una agenda

      Elena Azaola

      2004-01-01

      Full Text Available El trabajo analiza el linchamiento de tres agentes de la Policía Federal Preventiva que tuvo lugar en Tláhuac en noviembre de 2004 a la luz de los conflictos que puso de manifiesto tanto en la coordinación entre las instituciones de seguridad pública federales y locales así como en las relaciones entre policías y ciudadanos. Se enfoca el problema desde la perspectiva de la agenda de asuntos públicos por resolver para evitar que linchamientos como estos vuelvan a producirse.

    18. Calidad y desarrollo profesional del profesorado universitario, desde la perspectiva de las comunidades de aprendizaje

      Merma Molina, Gladys; Gavilán Martín, Diego

      2014-01-01

      En esta investigación, reflexionamos en torno a las competencias docentes que debe tener el profesor universitario desde la perspectiva de las comunidades de aprendizaje, en cada una de las etapas de la gestión del proceso de enseñanza-aprendizaje: 1) planificación y diseño de la docencia, donde se plantean competencias y objetivos, se organizan los contenidos, se eligen los materiales y recursos, y se formula la evaluación; 2) la puesta en acción de lo planificado, previamente (metodología y...

    19. La crianza como objeto de estudio actual desde el modelo transaccional

      María Cristina García; Marta Martínez

      2012-01-01

      El objetivo de este artículo es revisar cómo ha evolucionado la investigación enel tema de la crianza y cuáles son, desde el modelo transaccional, las perspectivas y metodologíasactuales. Este modelo plantea relaciones recíprocas entre padres o madres e hijos o hijas, considerandola influencia biológica en relación con la experiencia vital, para llegar a una teoría más complejasobre el desarrollo que la propuesta en las primeras investigaciones. Revisamos la importancia denuevas herramientas ...

    20. El lenguaje corporal, una práctica : Enfoque desde una educación corporal

      Galarza, Juliana Mariela

      2013-01-01

      En esta oportunidad la intención es presentar el estado de la cuestión con respecto a la investigación de Licenciatura en Educación Física en curso y se titula “El Lenguaje Corporal: una Práctica. Enfoque desde una Educación Corporal”. Cuestiones como el cuerpo, el movimiento, la enseñanza, el aprendizaje, son conceptos de uso frecuente donde se trata de poner en tensión distintos significados. Cada concepto se desprende para buscar unanimidad en el mejor ejercicio profesional. Llegar a de...

    1. La homosexualidad censurada: estudio sobre corpus de teatro TRACEti (desde 1960)

      Merino ??lvarez, Raquel

      2007-01-01

      p. 243-286 Con la perspectiva de los 29 a??os transcurridos desde la derogaci??n oficial de la censura teatral en Espa??a y con la acumulaci??n de datos que hemos ido recogiendo y analizando en los ??ltimos diez a??os de investigaciones sobre traducciones censuradas de teatro podemos hoy presentar un estudio de caso, Los chicos de la banda/The boys in the band, que ejemplifica c??mo se integraron en la escena espa??ola temas tab?? como el que nos ocupa. Se trata el tema de la homosexual...

    2. UNA MIRADA A LA ORIENTACION PROFESIONAL DESDE EL ENFOQUE HISTORICO CULTURAL EN LA EDUCACION SUPERIOR ACTUAL

      Odalia Llerena Companioni

      2011-01-01

      En el trabajo se aborda el significado que en las condiciones actuales tiene hacer orientación profesional en el contexto universitario desde el enfoque histórico - cultural del desarrollo humano. Se refiere que la orientación profesional puede ser una herramienta que permita enlazar los intereses individuales con los requisitos actuales de la sociedad acerca de la formación de profesionales. La orientación profesional no se restringe a los años de vida universitaria ni siquiera a los primero...

    3. Antropología, desarrollo e Interculturalidad: propuestas desde América Latina

      Cortés Maisonave, Almudena

      2014-01-01

      Este texto que introduce el monográfico recoge y plantea las aportaciones que la antropología social ha realizado desde América Latina en el análisis del desarrollo y la interculturalidad. El interés de esta tarea es doble. Por una parte, América Latina ha sido un laboratorio geopolítico privilegiado en la puesta en marcha de programas y proyectos de desarrollo, que se han exportado a otros lugares del planeta. Por otra parte, las contribuciones realizadas por la antropología latinoamericana ...

    4. El ecumenismo romano desde la perspectiva de la historiografía griega: Dionisio de Halicarnaso

      Plácido Sánchez, Domingo

      2008-01-01

      [ES] La figura de Dionisio de Halicarnaso representa un caso privilegiado para el estudio del ecumenismo romano, si se considera que se trata de un concepto recibido de la civilización griega, potenciado gracias al imperialismo romano que en gran medida servía de tema básico para su obra historiográfica. En la formación del Principado, vista desde la cultura griega, Dionisio de Halicarnaso se hace portavoz de la herencia que recoge la tradición de Roma como ciudad griega. En Dionisio se sinte...

    5. ES POSIBLE DESARROLLAR LA COMPETENCIA ARTÍSTICA Y CULTURAL DESDE LA QUÍMICA

      Mª Ángeles Sánchez Guadix

      2009-09-01

      Full Text Available Conseguir que el alumnado sea competente en todos los aspectos culturales de nuestra época es un objetivo muy loable. Lamentablemente, parece que las ciencias no puedan contribuir a dicho objetivo en los aspectos artísticos y culturales,ahondando de nuevo en la separación entre ciencia y cultura. En el presente artículo realizamos una reflexión, desde el punto de vista de la química, sobre dicha creencia para negarla.

    6. La talla de los europeos desde 1700: tendencias, ciclos y desigualdad

      Martínez-Carrión, José Miguel; Martínez Carrión, José Miguel

      2011-01-01

      En las últimas décadas los historiadores económicos exploran la talla humana para analizar los cambios seculares producidos en el bienestar biológico de las poblaciones y las relaciones que se establecen entre el crecimiento económico y el desarrollo humano. Los datos antropométricos son usados para complementar los conocimientos que tenemos sobre los niveles de vida desde la Revolución Industrial a partir de los salarios reales, la mortalidad, el consumo, entre otros indicadores. Con datos d...

    7. RESILIENCIA Y ENFERMEDAD MENTAL: UNA APROXIMACIÓN DESDE LA TERAPIA CENTRADA EN LA PERSONA

      Javier Armenta Mejía

      2010-01-01

      Este trabajo propone una exploración de la llamada "enfermedad mental" desde dos posturas que privilegian el desarrollo y el crecimiento sano del ser humano: la resiliencia y la terapia centrada en la persona. Se analizan algunos planteamientos del modelo médico, y, al mismo tiempo, se hace una crítica. Se presenta un modelo terapéutico basado en la teoría de Rogers, pero afín a ciertos planteamientos de la resiliencia.

    8. Medidas alternativas a la privación de libertad: enfoques desde el Trabajo Social

      Viviana Rodriguez Infante

      2005-01-01

      Este trabajo se enmarca dentro del análisis de las acciones alternativas a la privación de libertad en adolescentes infractores a la ley penal. Intenta buscar soluciones que eviten la cárcel para quienes cometan pequeños delitos, opción que puede disminuir el índice de reincidencias. El objetivo es promover, desde el Trabajo Social, una doble reflexión: primero, analizando críticamente el concepto de delito, su definición en las diversas corrientes ideológicas y su aplicación en la legislació...

    9. Gobierno de Barack Obama: una explicación desde el institucionalismo

      Javier Garay

      2010-01-01

      Este artículo trata de explicar, desde el institucionalismo, los problemas domésticos que ha tenido el gobierno de Barack Obama en los Estados Unidos. Se parte del hecho que este gobierno ha tenido una buena imagen en el ámbito internacional, pero ha tenido dificultades para generar consensos en el ámbito doméstico. Para esto, en el artículo se afirma que este gobierno demócrata se aleja de los valores, cultura y sociedad estadounidenses.

    10. Gobierno de Barack Obama: una explicación desde el institucionalismo

      Javier Garay

      2010-11-01

      Full Text Available Este artículo trata de explicar, desde el institucionalismo, los problemas domésticos que ha tenido el gobierno de Barack Obama en los Estados Unidos. Se parte del hecho que este gobierno ha tenido una buena imagen en el ámbito internacional, pero ha tenido dificultades para generar consensos en el ámbito doméstico. Para esto, en el artículo se afirma que este gobierno demócrata se aleja de los valores, cultura y sociedad estadounidenses.

    11. El Acuífero Guaraní desde el discurso mediático

      Hasperué, María del Rosario; Peredo, Carolina

      2009-01-01

      La noticia acerca de que el agua potable escasea en los principales centros industrializados del mundo puso en señal de alarma a ciertos países latinoamericanos ricos en este recurso natural vital y estratégico. La advertencia está referida a que ahora el agua dulce brota como factor de controversias geopolíticas y que la puja por su posesión y usufructo podría adoptar distintas modalidades que van desde la privatización hasta los enfrentamientos armados. En ese marco comenzaron los estudios ...

    12. Morelos: ¿un mito desmitificado? Una lectura del Martirio de Morelos desde la posmodernidad

      Martha Elia Arizmendi Domínguez; Jesús Humberto Florencia Zaldívar; Gerardo Meza García

      2012-01-01

      En Martirio de Morelos (1981), Vicente Leñero presenta, desde una perspectiva artística, parte de la vida y causa del llamado Siervo de la Nación, José María Morelos y Pavón. En esta pieza, el autor intercala aspectos históricos con detalles ficticios para lograr un efecto estético en los posibles lectores. Con genial agudeza, el autor recrea un momento histórico concreto, la guerra de Inde- pendencia de 1810. La obra posee un cauce de presentación narrativo, el cual se ve envuelto por la mod...

    13. Construcción de conocimiento desde los márgenes

      Dora Inés Múnevar

      2009-01-01

      Los sistemas de Ciencia y Tecnología (C&T), lo mismo que los de Investigación y Desarrollo (I+D), han definido formas de participación diferentes para mujeres y hombres, generando exclusiones por género. El campo de las epistemologías sociales analiza los límites de la educación, el trabajo académico o los intereses investigativos y formula preguntas desde los márgenes, en especial a la corriente principal. La perspectiva de género, devela las condiciones de p...

    14. Construcción de conocimiento desde los márgenes

      Múnevar, Dora Inés

      2009-01-01

      Los sistemas de Ciencia y Tecnología (C&T), lo mismo que los de Investigación y Desarrollo (I+D), han definido formas de participación diferentes para mujeres y hombres, generando exclusiones por género. El campo de las epistemologías sociales analiza los límites de la educación, el trabajo académico o los intereses investigativos y formula preguntas desde los márgenes, en especial a la corriente principal. La perspectiva de género, devela las condiciones ...

    15. Violencia en la enseñanza obligatoria : alternativas desde la perspectiva del profesorado

      Rodríguez Díaz, Francisco Javier; Gutiérrez Hernández, C.; Herrero Díez, Francisco Javier; Albuerne López, Ramón Fernando; Cuesta Izquierdo, Marcelino; Hernández Granda, Eva; Gómez Carbonero, Patricia; Jiménez Viñuela, María Asunción

      2004-01-01

      El objetivo de este artículo es establecer y conocer qué alternativas utiliza el profesorado para hacer frente a las situaciones de violencia escolar entre iguales, tanto aquellas que se producen en el aula como en el Centro, así como la oferta que se viene desarrollando desde su perspectiva a nivel de prevención. La muestra utilizada (n = 69 profesores de enseñanza obligatoria), tomada de diferentes Colegios de Enseñanza Primaria e IES de la Comunidad Autónoma del Principado de Asturias a tr...

    16. Falacias del desarrollo sustentable: una crítica desde la metamorfosis conceptual

      Daniel Murillo Licea

      2004-01-01

      Full Text Available Se revisan los orígenes de los conceptos de desarrollo rural y desarrollo sustentable desde un punto de vista en el que las ciencias del lenguaje, la semiótica y la comunicación entran en juego. Se presentan, a grandes rasgos, algunos elementos que aparecen en la determinación del uso y empleo de programas aplicados en México, así como dos ejemplos donde el desarrollo rural fue un pretexto para la aplicación y la actuación de algunas instituciones públicas.

    17. La autonomía, una visión desde el cuidado enfermero

      Busquets Surribas, Montserrat

      2004-01-01

      En este artículo trataremos el tema de la autonomía del paciente/usuario desde la visión del cuidado enfermero. La ética nos obliga a reflexionar sobre la bondad de nuestras acciones, sobre lo que consideramos más adecuado y nos obliga a ser capaces de justificar los motivos de todo ello. Por tanto, vamos a exponer las razones profesionales que nos llevan a definir la autonomía como el eje fundamental de las relaciones entre las enfermeras y los usuarios o enfermos, sea cual sea la condición ...

    18. Aprender a enseñar matemáticas desde la planificación

      Mora, Angela; Ortiz, José

      2013-01-01

      Se presenta un avance de investigación sobre el desarrollo de la competencia de planificación para aportar una visión sobre como aprender a enseñar matemáticas mediante la planificación. Es un estudio en curso, que se realiza desde el enfoque cualitativo interpretativo bajo la metodología de evaluación de programas. La observación y entrevista realizada a grupos de profesores en formación muestra: a) el análisis didáctico permite planificar una enseñanza centrada en el aprendizaje del estudia...

    19. La Biblioteca Tutorizada: Una Experiencia de Aprendizaje Dialógico desde una Comunidad de Aprendizaje

      Reca Fernández, María Henar

      2013-01-01

      Desde el proyecto comunidades de aprendizaje se pretende dar respuesta a las desigualdades imperantes en la sociedad de la información transformando las prácticas educativas tradicionales de la sociedad industrial a través de un planteamiento dialógico que utiliza como herramienta principal el aprendizaje dialógico. Dentro de las medidas de éxito educativo avaladas por la comunidad científica internacional desarrolladas en las comunidades de aprendizaje encontramos la biblioteca tutorizada. E...

    20. Ayudando a normalizar identificadores y perfiles públicos de investigador desde la Biblioteca

      Martínez Giménez, José Carlos

      2015-01-01

      Desde hace un tiempo se ha hecho evidente que existen, y en incremento, millones de personas investigando y produciendo información científica y técnica en forma continuada. Tiene una gran importancia intentar garantizar la univocidad en la identificación de los autores para una adecuada difusión, visibilidad y reconocimiento de las contribuciones que les son propias. Los identificadores de autor y sus perfiles públicos normalizados son una vía preferente para ello. Durante el último trime...

    1. La imagen de los destinos turísticos cubanos. Un acercamiento desde La Habana Vieja

      Dra. Maité Echarri Chávez; Msc. Lidié Perera Conde

      2012-01-01

      El estudio y análisis de la imagen de los destinos turísticos, a partir de las expectativas del cliente y desde la perspectiva de las entidades comercializadoras de los mismos, constituye todo un reto teniendo en cuenta que la decisión de viajar está también condicionada por un cúmulo de información que puede no ser correspondida con la realidad que se constata en el lugar de destino y los medios de comunicación. El informe que se presenta es resultado de investigaciones cuyo objetivo es cono...

    2. Contacto universitario: en el aire desde 1985

      Carrizo, Carlos; Di Marzio, Gustavo; Lima, Luis Julián; Castrogiovanni, Marcela

      1998-01-01

      Contacto universitario es el programa de difusión, análisis y debate de los temas de la UNLP y de la universidad en general. Creado y conducido por el periodista Carlos Carrizo, se emite por AM 1390 y FM 107.5 en diferentes horarios desde 1985. En esta entrega, la última emisión de 1998, se entrevista al dirigente de la Asociación de Docentes de la Universidad de La Plata (ADULP), Gustavo di Marzio; y luego, invitado en el estudio, al presidente de la UNLP de aquel entonces, Ingeniero Luis Ju...

    3. El cuidado integral de la salud mental desde el sector salud ante la violencia sexual

      Torres Gutiérrez, Nilda Carolina; Veloza Martí, Giovanna; Urrego Mendoza, Zulma Consuelo; Vejarano Velandia, Mónica; Cárdenas Rodríguez, Miguel Enrique

      2011-01-01

      6.1. El Cuidado de la Salud Mental desde el Sector Salud - 6.1.1 La Salud Mental - 6.1.1.2 Acciones de orientación esencial para un proceso de cuidado integral de la salud mental en personas y comunidades que sufren el impacto de la violencia sexual - 6.1.1.3 Impacto de la violencia sexual sobre la salud mental de las personas - 6.2. Implicaciones de la violencia sexual para la salud mental de las personas y las comunidades 6.2.1 Implicaciones psicológicas y sociales de la violencia sexual pa...

    4. Educación matemática desde la prensa escrita

      Muñoz Santonja, José

      1994-01-01

      Algunos profesores utilizan la prensa como recurso didáctico en sus clases. Suelen causar extrañeza. Mas, si el profesor es de Matemáticas, variopintos juicios pueden recaer sobre su acción educativa, porque ¿desde cuándo la prensa y las Matemáticas han tenido algo que ver? En este artículo se hacen algunas consideraciones al respecto que, ojalá, animen a los educadores matemáticos a introducir los medios de comunicación en general, y la prensa en particular, en los procesos de enseñanza y ap...

    5. Marketing y responsabilidad. Una aproximación ética desde René Girard

      Gómez-Ferrer Lozano, Guillermo

      2016-01-01

      La presente tesis tiene como objeto analizar la ética aplicada al marketing desde la perspectiva de la responsabilidad bajo el presupuesto de la hermenéutica crítica. La ética del marketing supone el descubrimiento y análisis de los principios, valores y fundamentos éticos en el campo del marketing y su función dentro de las organizaciones, así como las responsabilidades del mismo en sus ámbitos de acción e influencia. Para ello, hemos respondido en la primera parte, a qué es el marketing...

    6. Una propuesta didáctica desde el enfoque por competencias

      Chaves, Edwin; Castillo, Mario

      2011-01-01

      El presente artículo corresponde a un planteamiento didáctico para la enseñanza de la Estadística basado en la formación por competencias desde el pensamiento complejo. Debido a los tradicionales problemas que enfrenta la enseñanza de esta disciplina en el ámbito universitario, la cual se caracteriza por altos índices de deserción y reprobación de los estudiantes, se hace necesario buscar alternativas didácticas tendientes a revertir el proceso. La propuesta está dirigida hacia un módulo de E...

    7. Exploración sobre las decisiones estratégicas desde el pensamiento complejo.

      Luz María Rivas Montoya

      2013-01-01

      La racionalidad, las decisiones estratégicas y el pensamiento complejo motivaron la construcción del problema de esta investigación, planteado en términos del carácter limitado y reduccionista de los criterios y referentes de decisión en la visión gerencial tradicional frente a una visión compleja de la realidad humana. La exploración de este problema se realizó desde y para el campo académico de la estrategia, con el propósito de mostrar algunas de las dificultades que se presentan en la def...

    8. Familias acogedoras de menores. Un análisis desde el modelo de red social

      Poyatos García, Ana

      2014-01-01

      En el artículo se presenta el estudio realizado con sietes familias acogedoras de niños/as y jóvenes, en las modalidades de familia extensa y educadora. La investigación la hemos realizado desde la perspectiva ecosistémica basándonos para el análisis en el modelo de red social. Mostramos los datos mediante el mapa de las redes sociales formales e informales en el que analizamos cuatro áreas (familia, amistades, comunidad y los sistemas de salud servicios sociales, y el sistema edu...

    9. La pena de muerte desde la bioética y los derechos humanos

      Octavio Márquez Mendoza; Marcela Veytia López; Rosalinda Guadarrama Guadarrama; Sergio Ruiz Peña; Elba Margarita González Fabián

      2015-01-01

      Se ofrece una reflexión de la pena de muerte, que parte de premisas esenciales desde la bioética y los derechos humanos. Se plantean algunas generalidades de esta disciplina, dado que orientan a un cuestionamiento respecto al aporte de la ciencia y la tecnología para llevar a cabo "ejecuciones más humanas". Enseguida se recurre a los conceptos de vida, persona y dignidad para enmarcar el argumento en los derechos humanos. Se retoman algunas sentencias de documentos internacionales apelando a ...

    10. SALUD MENTAL: UN ABORDAJE DESDE LA PERSPECTIVA ACTUAL DE LA PSICOLOGÍA DE LA SALUD

      Moisés Mebarak; Alberto De Castro; María del Pilar Salamanca; María Fernanda Quintero

      2009-01-01

      Este artículo está basado en un estudio estado del arte acerca del entendimiento actual que se tiene sobre el concepto de salud mental, especialmente desde la perspectiva de la psicología de la salud. Con el fin de ilustrar este campo, se presentan algunos aportes y/o investigaciones tanto a nivel general como de la región costa Caribe colombiana. Se ha encontrado que el concepto de salud mental implica el desarrollo de estilos de vida y de características personales, interpersonales, sociale...

    11. LO SOCIAL EN LO ECONOMICO: UNA TOMA DE PULSO DESDE 1996

      Jeannette Cordero Gamboa

      2011-01-01

      A un paso de terminar el siglo XX, nos proponemos hacer una revisión rápida, desde 1996, a los resultados de las políticas sociales aplicadas especialmente en los años 90. Se pretende determinar si hay resultados que muestran un avance hacia un desarrollo humano sostenido en nuestro país en materia de salud, educación, ambiente y, en general, en calidad de vida.El propósito de la política económica supone efectos concretos que buscan aumentar el bienestar de la sociedad en la búsqueda de la e...

    12. Atención temprana y polidiscapacidad. Abordaje desde la discapacidad visual

      García-Trevijano Patrón, Cristina

      2010-01-01

      La autora plantea una perspectiva de la intervención en atención temprana con niños con deficiencias múltiples y discapacidad visual desde el punto de vista de su desarrollo global, es decir, abordando los aspectos comunes, delimitados por la presencia de un déficit visual grave. Propone el marco global de la evaluación, así como un análisis de los aspectos claves de la intervención, y los principales instrumentos y adaptaciones necesarios. Indica una serie de observaciones prácticas, dirigid...

    13. RESILIENCIA Y ENFERMEDAD MENTAL: UNA APROXIMACIÓN DESDE LA TERAPIA CENTRADA EN LA PERSONA

      Javier Armenta Mejía

      2010-01-01

      Full Text Available Este trabajo propone una exploración de la llamada "enfermedad mental" desde dos posturas que privilegian el desarrollo y el crecimiento sano del ser humano: la resiliencia y la terapia centrada en la persona. Se analizan algunos planteamientos del modelo médico, y, al mismo tiempo, se hace una crítica. Se presenta un modelo terapéutico basado en la teoría de Rogers, pero afín a ciertos planteamientos de la resiliencia.

    14. El cáncer de mama: desde la perspectiva de la experiencia

      Nieto Flaño, Rebeca

      2013-01-01

      La presente monografía trata el cáncer de mama desde la perspectiva de la experiencia. Está estructurada en cinco capítulos en función de los siguientes objetivos: describir la vivencia de las mujeres con cáncer de mama, explicar el impacto del diagnóstico en la familia, analizar el impacto de la nueva imagen corporal, explicar los beneficios de la participación en grupos de apoyo, y la importancia del uso de la metodología enfermera en el cuidado de estas pacientes. Las neoplasias de mama so...

    15. Efecto rizosférico de Parmelia sp y Solanum lixioides sobre actinomicetos y ensayos de antibiosis in vitro Rhizozpheric effect of Parmelia sp and Solanum lixioides on actinomycetes and their in vitro antimicrobial activity

      Zárate Lyda

      1999-11-01

      Full Text Available

      La presencia de actinomicetos en muestras de suelo alrededor de raíces y rizoides de Parmelia sp y de Solanum lixioides fue investigado. 5e evaluó el efecto rizosférico del liquen Parmelia sp sobre poblaciones de actinomicetos con un R/5 ~ 2.5, mientras el radio R/5 resultante en S.lixioides fue <1. En los ensayos de antibiosis los mejores resultados se obtuvieron al modificar el medio de cultivo, o medio mínimo de Hopwood (1967, con relación a medio estándar. Se determinó el espectro de actividad antibiótica de 9 aislamientos, sobre organismos Gram positivos: Staphylococcus aureus, Bacillus subtilis y Gram negativos: Escherichia coli, Klebsiella pneumoniae y Pseudomonas aeruginosa. Los aislamientos A-2, A-4, A-5 YA-6 presentaron un amplio espectro de actividad antimicrobiana sobre E. coli, B. subtilis y K. pneumoniae. A-11, presentó una antibiosis selectiva y en grado alto sobre P. aeruginosa.

      The occurrence of actinomycetes from rhizospheric soils of Parmelia sp and Solanum lixioides was investigated. The rhizospheric effect from Parmelia sp (Lichen upon actinomycetes isolates was evaluated and was (R/5 ~ 2.5, while by the solanaceae the resultant ratio R/5 was <1. For the antibiosis assays the better results were obtained by moditying the culture médium (Hopwood minimun medium, 1967. The antimicrobial activity from nine isolates against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae; Pseudomonas aeruginosa was determined. A-2, A-4, A-S and A-6 isolates showed a broad spectrum of antimicrobial activity, while A-'Il showed a selective antimicrobial activity against P. aeruginosa.

    16. Efficacy of fermenting liquor of one antagonistic actinomycete on experimental colibacillosis in chicken%一株拮抗放线菌发酵液对试验性鸡大肠杆菌病的疗效试验

      傅艳萍; 孙斌; 来航线; 王晶钰

      2009-01-01

      对一株具有抑菌效果的放线菌发酵液用于治疗鸡大肠杆菌病的疗效进行了研究.采用人工感染诱发鸡大肠杆菌病造模,分别以放线菌发酵液与氟苯尼考进行预防和治疗试验.结果显示,放线菌发酵液治疗组与氟苯尼考低剂量(5 mg/kg)组的大肠杆菌病治疗效果相当,差异不显著;放线菌发酵液预防组与感染对照组在死亡率、治愈率和增重方面差异显著.证实,试验所用的放线菌发酵液可用于治疗和预防人工诱发的O_(89)血清型鸡大肠杆菌病.%Avian colibacillosis induced by the experimental infection method was treated and prevented using the fermenting liquor of antagonistic actinomycetes and florfenieol. The fermenting liquor treatment group (5 mg/kg) had similar therapeutic effect to the low dose of florfenicol group,and the difference be-tween the two groups was not significant(P>0.05). The difference between the fermenting liquor preven-tion group and the infection control group was significant in mortality,cure rate,and weight gain. The re-sults showed that this fermenting liquor of actinomycete could treat and prevent O_(89) serotype avian coliba-cillosis induced by experimental infection.

    17. ENSEÑAR Y EL APRENDER LA HISTORIA DESDE LA MEMORIA HISTÓRICA

      Angel Felipe Jevey Vázquez

      2015-01-01

      Full Text Available Este artículo incursiona en una importante problemática relacionada con el tratamiento que ha recibido la memoria histórica como elemento esencial para enseñar y aprender la historia del lugar donde vivimos. La memoria histórica, desde nuestra visión, es uno de los componentes del patrimonio inmaterial de los pueblos que más requiere ser atendido y aprovechado en nuestras escuelas. Las potencialidades de la memoria histórica, como categoría cultural requiere ser conocida desde la ciencia y utilizada en la tarea educativa de la escuela. Estas razones hacen que este artículo tenga como objetivo explicar la importancia de la memoria histórica para la salvaguarda patrimonial de la historia personal, familiar, local y nacional, a partir de su relación con la gestión comunitaria de la escuela, y sobre la base de la contextualización educativa de esta categoría, tan necesaria en las actuales circunstancias ideológicas de la nación cubana.

    18. El concepto de calidad en las organizaciones: una aproximación desde la complejidad

      Pedro Emilio Sanabria Rangel

      2015-10-01

      Full Text Available Este artículo es el resultado de un trabajo de investigación que tiene como propósitos comprender los aportes del paradigma de la complejidad al discurso de la calidad, reivindicar la importancia de este concepto para el desarrollo de las organizaciones y contribuir a superar los límites existentes a la aplicación del mismo. El soporte epistemológico se encuentra en los aportes de las teorías de la complejidad, particularmente en aquellos desarrollados por Edgar Morin, un insigne impulsor de estas. Como resultado de la revisión realizada, y del análisis que de ella procede, se argumenta que la calidad no puede seguirse comprendiendo desde una visión mecanicista, sino que ella debe ser considerada desde una perspectiva compleja que incorpore, a su análisis y práctica, conceptos como los de incertidumbre, fluctuación, y otros más vinculados abiertamente con la misma.

    19. La imagen de los destinos turísticos cubanos. Un acercamiento desde La Habana Vieja

      Msc. Lidié Perera Conde

      2012-11-01

      Full Text Available El estudio y análisis de la imagen de los destinos turísticos, a partir de las expectativas del cliente y desde la perspectiva de las entidades comercializadoras de los mismos, constituye todo un reto teniendo en cuenta que la decisión de viajar está también condicionada por un cúmulo de información que puede no ser correspondida con la realidad que se constata en el lugar de destino y los medios de comunicación. El informe que se presenta es resultado de investigaciones cuyo objetivo es conocer y analizar esas expectativas para el caso cubano, así como el análisis de la imagen inducida desde la perspectiva de las entidades comercializadoras, tomando como referente La Habana Vieja, principal destino de ciudad de Cuba. Los resultados obtenidos permitieron reconocer los íconos culturales que identifican a la ciudad y el papel que los mismos juegan en los diferentes soportes comunicacionales, proporcionándoles a las entidades gestoras de la actividad turística el punto de vista adecuado para la futura toma de decisiones en el ámbito de la imagen.

    20. La ciudad desde los imaginarios urbanos del sabor: Tunja como caso de estudio

      Carlos Mario Rodríguez

      2015-06-01

      Full Text Available El artículo presenta los resultados generales de la investigación “Sabores de la ciudad imaginada”, realizada en la ciudad de Tunja, Colombia . Este estudio, hecho desde un enfoque cualitativo y bajo la metodología de análisis de los imaginarios urbanos de la ciudad, se propuso el desarrollo de una lectura de ciudad desde la perspectiva de los habitantes, a partir de la premisa ¿Cuál es el sabor de Tunja? El abordaje al ciudadano se planteó mediante el diseño de tres instrumentos: encuesta, cuestionario e intervención artística, de esta manera, se desarrolló un análisis de las percepciones del sentido del gusto con relación a la ciudad, lo cual demostró que los imaginarios urbanos son expresados, también, por medio de la percepción del sabor y que su análisis permite reconocer relaciones establecidas entre sabores y lugares que tienen que ver con el uso que se le da a los espacios, las rutinas, formas de actuar e ideas comúnmente aceptadas con relación a las características de la ciudad.