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Sample records for actinobacillus lignieresii

  1. Final classification of Bisgaard taxon 9 as Actinobacillus arthritidis sp nov and recognition of a novel genomospecies for equine strains of Actinobacillus lignieresii

    Christensen, Henrik; Bisgaard, Magne; Angen, Øystein; Olsen, John Elmerdahl

    2002-01-01

    Phenotypic characterization of bacteria from diseased and healthy horses identified 18 isolates as Bisgaard taxon 9 and 11 isolates as Actinobacillus lignieresii. All strains of taxon 9 were alpha-galactosidase- and raffinose-positive and showed variable fermentation of (+)L-arabinose and (-)D-sorbitol....... Strains of A. lignieresii were negative for these characteristics, with the exception of raffinose. Two strains from the (-)D-sorbitol-negative group of taxon 9 showed a 16S rRNA similarity of 99.6%, while 99.5% similarity was found between two strains of the (-)D-sorbitol-positive group. DNA......-DNA hybridization between the two strains representing the (-)D-sorbitol-negative group showed 98% binding, and their closest relationship was to a strain of A. lignieresii (64%). The two strains of the (-)D-sorbitol-positive group showed 83% binding and were related to the (-)D-sorbitol-negative group at a 76% DNA...

  2. Delineation of the genus Actinobacillus by comparison of partial infB sequences

    Nørskov-Lauritsen, Niels; Christensen, H; Okkels, H.; Kilian, Mogens; Bruun, B

    2004-01-01

    position of Actinobacillus capsulatus was unresolved; this species is more remotely related to A. lignieresii. The two species A. lignieresii and A. pleuropneumoniae could not be clearly separated by infB sequence analysis. The phylogeny of the genus Actinobacillus based on infB analysis was essentially......, Actinobacillus pleuropneumoniae, Actinobacillus equuli, Actinobacillus suis, Actinobacillus ureae, Actinobacillus arthritidis, Actinobacillus hominis and two unnamed genomospecies showed more than 85 % similarity to the type strain of the type species of the genus, Actinobacillus lignieresii. The taxonomic...... congruent with relationships inferred from 16S rRNA sequence comparisons and DNA hybridization studies. Discrepancies were encountered with single strains or taxa at the periphery of the genus. Greater intraspecies variation was observed with infB sequences than with 16S rRNA gene sequences, with notable...

  3. Genetic diversity of Actinobacillus lignieresii isolates from different hosts

    Kokotovic, Branko; Angen, Øystein; Bisgaard, Magne

    2011-01-01

    strains isolated from horses and infected wounds of humans bitten by horses and another consisting of strains isolated from bovine and ovine hosts. The present data indicate a comparatively higher degree of genetic diversity among strains isolated from equine hosts and confirm the existence of a separate...... genomospecies for A. lignieresi-like isolates from horses. Among the isolates from bovine and ovine hosts some clonal lines appear to be genetically stable over time and could be detected at very distant geographic localities. Although all ovine strains investigated grouped in a single cluster, the existence of...... distinct genetic lineages that have evolved specificity for ovine hosts is not obvious and needs to be confirmed in other studies....

  4. The transferrin receptor of Actinobacillus pleuropneumoniae: Quantitation of expression and structural characterization using a peptide-specific monoclonal antibody

    Bøg, Yang S.; Andresen, Lars Ole; Bastholm, L.;

    2001-01-01

    antigens were detected with the Mab in iron-starved Actinobacillus lignieresii, Actinobacillus porcinus, Actinobacillus minor Haemophilus influenzae. and Haemophilus parasuis. Using an enzyme-linked immunosorbent assay (ELISA) based on the Mab 1.48, Tbp2 could be detected in both recombinant E. coli......-restricted culture. It was found that Tbp2 was not expressed in iron replete medium by any serotype except serotypes 5a, 5b and 6 where a weak expression was seen. There was a weak expression of related antigens in Actinobacillus indolicus and Actinobacillus suis under iron-depleted conditions while no similar...... expressing Tbp2 and in wild type A. pp grown under iron restricted conditions. The subcellular location of Tbp2 in A. pp was studied by immunoelectron microscopy using the Mab 1.48. Interestingly, all antibody binding was found inside the A. pp cells, while Tbp2 expressed in recombinant E. coli was found...

  5. Evaluation of a PCR for detection of Actinobacillus pleuropneumoniae in mixed bacterial cultures from tonsils

    Gram, T.; Ahrens, Peter; Nielsen, J.P.

    1996-01-01

    strains of A. lignieresii. The lower detection limit of the PCR test was 10(3) A. pleuropneumoniae CFU/PCR test tube and was not affected by addition of 10(6) E. coli CFU/PCR test tube. Mixed bacterial cultures from tonsils of 101 pigs from 9 different herds were tested by culture and by PCR using four...... different bacteriological media. While 65% reacted positive; in the PCR only 23% were positive by culture, thereby suggesting a superior sensitivity of the PCR test to that of culture. The use of selective media, large inoculum and incubation for 48 h gave the highest number of positive PCR reactions from......A PCR for the detection of Actinobacillus pleuropneumoniae was evaluated. All of 102 field isolates of A. pleuropneumoniae reacted in the PCR by amplification of a 985 bp product. No PCR amplification product was observed when examining strains of A. ureae, A. capsulatus, A. hominis, A. equuli, A...

  6. Clinical significance and taxonomy of Actinobacillus hominis

    Friis-Møller, Alice; Christensen, J J; Fussing, V;

    2001-01-01

    Clinical findings in 36 immunosuppressed patients with lower respiratory tract infection or bacteremia with Actinobacillus hominis are described. Animal contact was only recorded for three patients; nine patients died despite appropriate antimicrobial treatment. Although infections with this micr...

  7. Clinical significance and taxonomy of Actinobacillus hominis

    Friis-Møller, Alice; Christensen, J J; Fussing, V;

    2001-01-01

    microorganism seem to be rare, the fact that 37 of 46 strains characterized in this study have been found in Copenhagen indicates that under-reporting may occur. A. hominis is phenotypically relatively homogeneous but can be difficult to differentiate from other Actinobacillus species unless extensive...

  8. Actinobacillus pleuropneumoniae transmission and clinical outbreaks

    Tobias, T.J.

    2014-01-01

    Actinobacillus pleuropneumoniae is a bacterium causing respiratory disease and mortality in pigs. Outbreaks of clinical disease occur regularly in pig farms. More knowledge of the epidemiology of the bacteria in pig populations should contribute to the design of more effective measures for preventio

  9. Selective medium for isolation of Actinobacillus actinomycetemcomitans.

    Slots, J

    1982-01-01

    A selective medium, TSBV (tryptic soy-serum-bacitracin-vancomycin) agar, was developed for the isolation of Actinobacillus actinomycetemcomitans, TSBV agar contained (per liter) 40 g of tryptic soy agar, 1 g of yeast extract, 100 ml of horse serum. 75 mg of bacitracin, and 5 mg of vancomycin. The TSBV medium suppressed most oral species and permitted significantly higher recovery of A. actinomycetemcomitans than nonselective blood agar medium. The distinct colonial morphology and positive cat...

  10. Binding of Actinobacillus pleuropneumoniae to Phosphatidylethanolamine

    Jeannotte, Marie-Eve; Abul-Milh, Maan; Dubreuil, J. Daniel; Jacques, Mario

    2003-01-01

    The gram-negative bacterium Actinobacillus pleuropneumoniae is the causative agent of porcine fibrinohemorrhagic necrotizing pleuropneumonia, a disease that causes important economic losses to the swine industry worldwide. In general, the initial step of bacterial colonization is attachment to host cells. The purpose of the present study was to evaluate the binding of A. pleuropneumoniae serotype 1 to phospholipids, which are the major constituents of biological membranes. Phospholipids serve...

  11. Fluoroquinolones in the treatment of Actinobacillus actinomycetemcomitans associated periodontitis

    Kleinfelder, JW; Mueller, RF; Lange, DE

    2000-01-01

    Background: Periodontitis patients harboring Actinobacillus actinmycetemcomitans (Aa) are prime candidates for systemic antibiotic therapy. Besides tetracycline and the combination of metronidazole and amoxicillin the fluoroquinolones are also believed to have antibacterial activity against Aa. The

  12. Survival of Actinobacillus pleuropneumoniae outside the pig.

    Assavacheep, P; Rycroft, A N

    2013-02-01

    Transmission of Actinobacillus pleuropneumoniae is primarily thought to be via direct transfer of mucus from pig to pig. For transfer between farms, the organism may need to persist in the wet or dried state to be carried on an inanimate surface. The survival of A. pleuropneumoniae was investigated under controlled laboratory conditions. In aqueous suspension, survival was improved by the presence of NaCl and mucin; it was prolonged at lower temperature. In dry state, it survived best on a hydrophobic surface either under desiccated conditions or saturated humidity. Detectable viability was maintained for 3-4 days. When frozen, A. pleuropneumoniae survived for more than 17 weeks at -20 °C, but the viability declined to 0.01% during that time. Survival at -70 °C was effective for long term storage. Results obtained from this investigation would be applicable for sampling method, transport techniques, epidemiological study, and biosecurity implementation. PMID:22892250

  13. Isolation of Actinobacillus suis from a cat's lung

    Daignault, D.; Chouinard, L.; Møller, Kristian;

    1999-01-01

    Actinobacillus suis has been isolated from the lungs of a 9-month-old cat. The bacterium was characterized biochemically as well as genetically, and its sensitivity profile to different antimicrobial agents was established. The role of this isolate in the cat's condition is discussed....

  14. A cohort study on Actinobacillus pleuropneumoniae colonisation in suckling piglets

    Tobias, T.J.; Klinkenberg, D.; Bouma, A.; Broek, van den J.; Daemen, A.J.J.M.; Wagenaar, J.A.; Stegeman, J.A.

    2014-01-01

    Actinobacillus pleuropneumoniae causes respiratory disease in pigs and despite the use of preventive measures such as vaccination and antimicrobials clinical outbreaks still occur. At weaning often many piglets are not colonised. If differences in prevalence between litters are large and if factors

  15. Identification of Genes Coding for Exported Proteins of Actinobacillus actinomycetemcomitans

    Mintz, Keith P.; Fives-Taylor, Paula M.

    1999-01-01

    Random fusions of genomic DNA fragments to a partial gene encoding a signal sequence-deficient bacterial alkaline phosphatase were utilized to screen for exported proteins of Actinobacillus actinomycetemcomitans in Escherichia coli. Twenty-four PhoA+ clones were isolated and sequenced. Membrane localization signals in the form of signal sequences were deduced from most of these sequences. Several of the deduced amino acid sequences were found to be homologous to known exported or membrane-ass...

  16. Adhesion of Actinobacillus actinomycetemcomitans to a human oral cell line.

    Mintz, K. P.; Fives-Taylor, P M

    1994-01-01

    Two quantitative, rapid assays were developed to study the adhesion of Actinobacillus actinomycetemcomitans, an oral bacterium associated with periodontal disease, to human epithelial cells. The human oral carcinoma cell line KB was grown in microtiter plates, and adherent bacteria were detected by an enzyme-linked immunosorbent assay with purified anti-A. actinomycetemcomitans serum and horseradish peroxidase-conjugated secondary antibody or [3H]thymidine-labeled bacteria. Adhesion was found...

  17. Requirements for invasion of epithelial cells by Actinobacillus actinomycetemcomitans.

    Sreenivasan, P K; Meyer, D H; Fives-Taylor, P M

    1993-01-01

    Actinobacillus actinomycetemcomitans, an oral bacterium implicated in human periodontal disease, was recently demonstrated to invade cultured epithelial cells (D. H. Meyer, P. K. Sreenivasan, and P. M. Fives-Taylor, Infect. Immun. 59:2719-2726, 1991). This report characterizes the requirements for invasion of KB cells by A. actinomycetemcomitans. The roles of bacterial and host factors were investigated by using selective agents that influence specific bacterial or host cell functions. Inhibi...

  18. Comparison of six typing methods for Actinobacillus actinomycetemcomitans.

    van Steenbergen, T J; Bosch-Tijhof, C J; van Winkelhoff, A J; Gmür, R; de Graaff, J

    1994-01-01

    Actinobacillus actinomycetemcomitans is an important pathogen in the etiology of severe periodontitis. For epidemiological studies on the prevalence of certain pathogenic clones and transmission of this bacterium, adequate typing methods are necessary. The purpose of this study was to compare six different typing methods for A. actinomycetemcomitans. Five reference strains and 27 fresh clinical isolates from periodontitis patients were used. Serotyping showed 12 serotype a strains, 13 type b ...

  19. Oxidative and nonoxidative killing of Actinobacillus actinomycetemcomitans by human neutrophils.

    Miyasaki, K T; Wilson, M E; Brunetti, A J; Genco, R J

    1986-01-01

    Actinobacillus actinomycetemcomitans is a facultative gram-negative microorganism which has been implicated as an etiologic agent in localized juvenile periodontitis and in subacute bacterial endocarditis and abscesses. Although resistant to serum bactericidal action and to oxidant injury mediated by superoxide anion (O2-) and hydrogen peroxide (H2O2), this organism is sensitive to killing by the myeloperoxidase-hydrogen peroxide-chloride system (K.T. Miyasaki, M.E. Wilson, and R.J. Genco, In...

  20. Isolation and identification of Actinobacillus pleuropneumoniae in pig's lungs at farms and their sensitivity to antibiotics

    Žutić Milenko; Ašanin Ružica; Milić N.; Ivetić V.; Vidić Branka; Žutić Jadranka; Ašanin Jelena

    2008-01-01

    The presence of Actinobacillus pleuropneumoniae has been established in all suspected cases of pleuropneumonia at several farms that have been included in the research. Equal incidence of pleuropneumonia has been found both among piglets for breeding and for fattening. The health monitoring of herds is extremely important, firstly because of the need for the adequate strategy to be chosen for controlling the Actinobacillus-caused pleuropneumonia and, at the same time, in order to prevent enor...

  1. Actinobacillus suis and Actinobacillus equuli, emergent pathogens of septic embolic nephritis, a new challenge for the swine industry Actinobacillus suis y Actinobacillus equuli, patógenos emergentes de nefritis embólica séptica, un nuevo desafío para la industria porcina

    CE Benavente; IC Fuentealba

    2012-01-01

    Kidney lesions are an important cause of tissue condemnation in slaughterhouses. In addition to the potential public health implications, organ condemnations have a significant economic impact on the food animal industry. The condition classified broadly as "nephritis" is one of the main causes of tissue condemnation. Embolic nephritis resembling Actinobacillus equuli infection in foals has been recently detected in sows and market hogs. Actinobacillus suis is phenotypically and phylogenetica...

  2. Evaluation of 5 ' nuclease assay for detection of Actinobacillus pleuropneumoniae

    Angen, Øystein; Jensen, J.; Lavritsen, D. T.

    2001-01-01

    , nonspecific reactions appeared when testing dilutions of DNA templates or pure cultures of A. pleuropneumoniae, as well as when testing tonsil scrapings from specific-pathogen-free herds. The diagnostic sensitivity, as evaluated with 586 tonsil scrapings from animals infected with A. pleuropneumoniae, is the...... equal to 30 must be chosen in order to obtain reliable results. The investigation emphasizes that a thorough evaluation of the criteria used to define a positive test result is necessary.......Sequence detection by the 5' nuclease TaqMan assay uses online detection of internal fluorogenic probes in closed PCR tubes. Primers and probe were chosen from a part of the omlA gene common to all serotypes of Actinobacillus pleuropneumoniae, which gave an amplicon of 92 bp, The test was evaluated...

  3. Characterization of bifunctional L-glutathione synthetases from Actinobacillus pleuropneumoniae and Actinobacillus succinogenes for efficient glutathione biosynthesis.

    Yang, Jianhua; Li, Wei; Wang, Dezheng; Wu, Hui; Li, Zhimin; Ye, Qin

    2016-07-01

    Glutathione (GSH), an important bioactive substance, is widely applied in pharmaceutical and food industries. In this work, two bifunctional L-glutathione synthetases (GshF) from Actinobacillus pleuropneumoniae (GshFAp) and Actinobacillus succinogenes (GshFAs) were successfully expressed in Escherichia coli BL-21(DE3). Similar to the GshF from Streptococcus thermophilus (GshFSt), GshFAp and GshFAs can be applied for high titer GSH production because they are less sensitive to end-product inhibition (Ki values 33 and 43 mM, respectively). The active catalytic forms of GshFAs and GshFAp are dimers, consistent with those of GshFPm (GshF from Pasteurella multocida) and GshFSa (GshF from Streptococcus agalactiae), but are different from GshFSt (GshF from S. thermophilus) which is an active monomer. The analysis of the protein sequences and three dimensional structures of GshFs suggested that the binding sites of GshFs for substrates, L-cysteine, L-glutamate, γ-glutamylcysteine, adenosine-triphosphate, and glycine are highly conserved with only very few differences. With sufficient supply of the precursors, the recombinant strains BL-21(DE3)/pET28a-gshFas and BL-21(DE3)/pET28a-gshFap were able to produce 36.6 and 34.1 mM GSH, with the molar yield of 0.92 and 0.85 mol/mol, respectively, based on the added L-cysteine. The results showed that GshFAp and GshFAs are potentially good candidates for industrial GSH production. PMID:26996628

  4. Phylogenetic relationship of equine Actinobacillus species and distribution of RTX toxin genes among clusters

    Kuhnert, Peter; Berthoud, Hélène; Christensen, Henrik; Bisgaard, Magne; Frey, Joachim

    2003-01-01

    Equine Actinobacillus species were analysed phylogenetically by 16S rRNA gene (rrs) sequencing focusing on the species Actinobacillus equuli, which has recently been subdivided into the non-haemolytic A. equuli subsp. equuli and the haemolytic A. equuli subsp. haemolyticus. In parallel we determined the profile for RTX toxin genes of the sample of strains by PCR testing for the presence of the A. equuli haemolysin gene aqx, and the toxin genes apxI, apxII, apxIII and apxIV, which are known in...

  5. Actinobacillus Actinomycetemcomitans y Porphyromonas Gingivales como principales patógenos periodontales

    A Bascones

    2000-09-01

    Full Text Available Entre las bacterias relacionadas con la enfermedad periodontal, existen dos especies más claramente asociadas a esta enfermedad: Actinobacillus actinomycetemcomitans y Porphyromonas gingivalis. Este trabajo es una revisión bibliográfica sobre estos dos patógenos periodontales, mostrando su origen, prevalencia, distribución, transmisión y respuesta al tratamiento periodontal.Among the bacteria related to periodontal disease, there are two species clearly associated to this disease: Actinobacillus actinomycetemcomitans and Porphyromonas gingiva lis. This paper presents a review of the literature regarding this two periodontal pathogens, and showing their origin, prevalence, distribution, transmission and response to periodontal treatment.

  6. Economical succinic acid production from cane molasses by Actinobacillus succinogenes.

    Liu, Yu-Peng; Zheng, Pu; Sun, Zhi-Hao; Ni, Ye; Dong, Jin-Jun; Zhu, Lei-Lei

    2008-04-01

    In this work, production of succinic acid by Actinobacillus succinogenes CGMCC1593 using cane molasses as a low cost carbon source was developed. In anaerobic bottles fermentation, succinic acid concentration of 50.6+/-0.9 g l(-1) was attained at 60 h using an optimum medium containing molasses pretreated with sulfuric acid, resulting in a succinic acid yield of 79.5+/-1.1% and sugar utilization of 97.1+/-0.6%. When batch fermentation was carried out in a 5-l stirred bioreactor with pretreated molasses, 46.4 g l(-1) of succinic acid was attained at 48 h and faster cells growth was also observed. Fed batch fermentation was performed to minimize the substrate (sugar) inhibition effect, giving 55.2 g l(-1) of succinic acid and 1.15 g l(-1)h(-1) of productivity at 48 h. The present study suggests that the inexpensive cane molasses could be utilized for the economical and efficient production of succinic acid by A. succinogenes. PMID:17532626

  7. Catecholamines promote Actinobacillus pleuropneumoniae growth by regulating iron metabolism.

    Lu Li

    Full Text Available Catecholamines are host stress hormones that can induce the growth of many bacteria by facilitating iron utilization and/or regulate the expression of virulence genes through specific hormone receptors. Whether these two responsive pathways are interconnected is unknown. In our previous study, it was found that catecholamines can regulate the expression of a great number of genes of Actinobacillus pleuropneumoniae, an important swine respiratory pathogen. However, bacterial growth was not affected by catecholamines in rich medium. In this study, it was discovered that catecholamines affected A. pleuropneumoniae growth in chemically defined medium (CDM. We found that serum inhibited A. pleuropneumoniae growth in CDM, while epinephrine, norepinephrine and dopamine promoted A. pleuropneumoniae growth in the CDM containing serum. The known bacterial hormone receptor QseC didn't play roles in this process. Ion-supplementation and transcriptome analysis indicated that serum addition resulted in iron-restricted conditions which were alleviated by the addition of catecholamines. Transferrin, one of the components in serum, inhibited the growth of A. pleuropneumoniae in CDM, an effect reversed by addition of catecholamines in a TonB2-dependent manner. Our data demonstrate that catecholamines promote A. pleuropneumoniae growth by regulating iron-acquisition and metabolism, which is independent of the adrenergic receptor QseC.

  8. Evaluation of 5 ' nuclease assay for detection of Actinobacillus pleuropneumoniae

    Angen, Øystein; Jensen, J.; Lavritsen, D. T.

    2001-01-01

    Sequence detection by the 5' nuclease TaqMan assay uses online detection of internal fluorogenic probes in closed PCR tubes. Primers and probe were chosen from a part of the omlA gene common to all serotypes of Actinobacillus pleuropneumoniae, which gave an amplicon of 92 bp, The test was evaluated...... with 73 lung isolates and 120 tonsil isolates of A. pleuropneumoniae as well as with a collection of reference strains. By using a C-t value (cycle number in which the fluorescence exceeds the threshold defined by the software) of 30 as the cutoff limit, the 5' nuclease assay represents a test with 100......% sensitivity and 100% specificity, A high degree of reproducibility of the test was demonstrated. If samples with C-t values of less than or equal to 30 are considered positive, the detection limit of the assay was 1 CPU/reaction tube, corresponding to a 10-fold higher number of DNA templates. After cycle 30...

  9. Serotyping of Actinobacillus pleuropneumoniae serotype 5 strains using a monoclonal-based polystyrene agglutination test

    Dubreuil, J.D.; Letellier, A.; Stenbæk, Eva; Gottschalk, M.

    1996-01-01

    A polystyrene agglutination test has been developed for serotyping Actinobacillus pleuropneumoniae serotype 5a and 5b strains. Protein A-coated polystyrene microparticles were sensitized with a murine monoclonal antibody recognizing an epitope on serotype 5 LPS-O chain as shown by SDS-PAGE and...

  10. Serological characterization of Actinobacillus pleuropneumoniae biotype 1 strains antigenically related to both serotypes 2 and 7

    Nielsen, R.; Andresen, Lars Ole; Plambeck, Tamara

    1996-01-01

    Nine Danish Actinobacillus pleuropneumoniae biotype 1 isolates were shown by latex agglutination and indirect haemagglutination to possess capsular polysaccharide epitopes identical to those of serotype 2 strain 1536 (reference strain of serotype 2) and strain 4226 (Danish serotype 2 strain). Imm...

  11. Synergistic effects between amoxicillin, metronidazole, and the hydroxymetabolite of metronidazole against Actinobacillus actinomycetemcomitans.

    Pavicić, M J; van Winkelhoff, A J; de Graaff, J

    1991-01-01

    Interactions between metronidazole and amoxicillin, metronidazole and its hydroxymetabolite, and amoxicillin and the hydroxymetabolite of metronidazole were investigated with checkerboard titrations in combination with accurately determined MICs and MBCs. Actinobacillus actinomycetemcomitans was used as the test organism. Synergism was found for all three combinations. Fractional inhibitory concentration indices and fractional bactericidal concentration indices varied from 0.3 to 0.7. These s...

  12. Experimental vaccination of pigs with an Actinobacillus pleuropneumoniae serotype 5b capsular polysaccharide tetanus toxoid conjugate

    Andresen, Lars Ole; Jacobsen, M.J.; Nielsen, J.P.

    1997-01-01

    The protective efficacy of an Actinobacillus pleuropneumoniae serotype 5b capsular polysaccharide-tetanus toroid conjugate (Ap5bCP-TT) against homologous challenge of pigs was investigated. Four pigs were non-vaccinated controls (group A), 4 pigs were injected with adjuvant without antigen (group B...

  13. Experimental vaccination of pigs with an Actinobacillus pleuropneumoniae serotype 5b capsular polysaccharide tetanus toxoid conjugate

    Andresen, Lars Ole; Jacobsen, M.J.; Nielsen, J.P.

    1997-01-01

    The protective efficacy of an Actinobacillus pleuropneumoniae serotype 5b capsular polysaccharide-tetanus toroid conjugate (Ap5bCP-TT) against homologous challenge of pigs was investigated. Four pigs were non-vaccinated controls (group A), 4 pigs were injected with adjuvant without antigen (group...

  14. Concurrent host-pathogen gene expression in the lungs of pigs challenged with Actinobacillus pleuropneumoniae

    Brogaard, Louise; Schou, Kirstine Klitgaard; Heegaard, Peter M. H.;

    2015-01-01

    Background: Actinobacillus pleuropneumoniae causes pleuropneumonia in pigs, a disease which is associated with high morbidity and mortality, as well as impaired animal welfare. To obtain in-depth understanding of this infection, the interplay between virulence factors of the pathogen and defense ...

  15. Multiplex PCR that can distinguish between immunologically cross-reactive serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains

    Zhou, L.; Jones, S.C.P.; Angen, Øystein; Bosse, J.T.; Nash, J. H. E.; Frey, J.; Zhou, R.; Chen, H.C.; Kroll, J.S.; Rycroft, A.N.; Langford, P.R.

    2008-01-01

    We describe a highly sensitive and specific multiplex PCR, based on capsular loci and the species specific apxIV gene, that unequivocally differentiates serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains that are cross-reactive in conventional immunological tests.......We describe a highly sensitive and specific multiplex PCR, based on capsular loci and the species specific apxIV gene, that unequivocally differentiates serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains that are cross-reactive in conventional immunological tests....

  16. Inokulasi Bakteri Selulolitik Actinobacillus sp. Asal Rumen pada Daun Jati Menurunkan Serat Kasar dan Meningkatkan Protein Kasar

    Mirni Lamid

    2013-11-01

    Full Text Available Constraints use teak leaves as ruminant feed is a high content of crude fiber and low crude protein.The objective of this research was to determine potency of inoculation  Actinobacillus sp. of rumen productionon teak leaves fermentation process could improve quality of teak leaves as animal feed for ruminants.Design study was Completely Randomized Design with four treatments and five replications. Fourtreatment groups were consist of  : P0 = teak leaves + 2% molasses (control; P1 = teak leaves + 2%molasses + 5% Actinobacillus sp.;  P2 = teak leaves + 2% molasses + 10% Actinobacillus sp.;  P3 = teakleaves + 2% molasses + 15%  Actinobacillus sp. Proximate analysis were done after teak leaves werefermented for seven days. The data were analyzed with Analysis of Variance followed by Duncan’s MultipleRange Test. The results showed a significant difference (P <0.05 for crude fiber and crude protein P3 andP2 compared with control’s. Crude fiber content of P3 and P2 respectively 31.67% and 32%, while the crudeprotein content of P3 and P2 respectively 13.57% and 13.31%. Conclusions of this research are:  InoculationActinobacillus sp. with doses 5%, 10% and 15% in the fermentation teak leaves can decrease crude fibercontent and increased crude protein content. Dose efficient to use bacterial fermentation teak leavesActinobacillus sp. is 10%.

  17. Profiling microRNAs in lung tissue from pigs infected with Actinobacillus pleuropneumoniae

    Podolska, Agnieszka; Anthon, Christian; Bak, Mads; Tommerup, Niels; Skovgaard, Kerstin; Heegaard, Peter M. H.; Gorodkin, Jan; Cirera, Susanna; Fredholm, Merete

    2012-01-01

    still very limited. Results: In this study, the RNA extracted from visually unaffected and necrotic tissue from pigs infected with Actinobacillus pleuropneumoniae was subjected to small RNA deep sequencing. We identified 169 conserved and 11 candidate novel microRNAs in the pig. Of these, 17 were......Background: MicroRNAs (miRNAs) are a class of non-protein-coding genes that play a crucial regulatory role in mammalian development and disease. Whereas a large number of miRNAs have been annotated at the structural level during the latest years, functional annotation is sparse. Actinobacillus...... pleuropneumoniae (APP) causes serious lung infections in pigs. Severe damage to the lungs, in many cases deadly, is caused by toxins released by the bacterium and to some degree by host mediated tissue damage. However, understanding of the role of microRNAs in the course of this infectious disease in porcine is...

  18. Autoinducer 2 Is Required for Biofilm Growth of Aggregatibacter (Actinobacillus) actinomycetemcomitans▿

    Shao, HanJuan; Lamont, Richard J.; Demuth, Donald R.

    2007-01-01

    Autoinducer 2 (AI-2) is required for the growth of Aggregatibacter (Actinobacillus) actinomycetemcomitans in culture under conditions of iron limitation. However, in vivo this organism thrives in a complex multispecies biofilm that forms in the human oral cavity. In this report, we show that adherent growth of A. actinomycetemcomitans on a saliva-coated surface, but not planktonic growth under iron-replete conditions, is defective in a LuxS-deficient background. Biofilm growth of the luxS mut...

  19. PCR Methods for Rapid Identification and Characterization of Actinobacillus seminis Strains

    Appuhamy, S; Coote, J G; Low, J. C.; Parton, R

    1998-01-01

    Twenty-four isolates of Actinobacillus seminis were typed by PCR ribotyping, repetitive extragenic palindromic element (REP)-based PCR, and enterobacterial repetitive intergenic consensus (ERIC)-based PCR. Five types were distinguished by REP-PCR, and nine types were distinguished by ERIC-PCR. PCR ribotyping produced the simplest pattern and could be useful for identification of A. seminis and for its differentiation from related species. REP- and ERIC-PCR could be used for strain differentia...

  20. Evidence for invasion of a human oral cell line by Actinobacillus actinomycetemcomitans.

    Meyer, D H; Sreenivasan, P K; Fives-Taylor, P M

    1991-01-01

    Actinobacillus actinomycetemcomitans, an oral bacterial species associated with periodontal disease, was found to invade human cell lines. Invasion was demonstrated by recovery of viable organisms from gentamicin-treated KB cell monolayers and by light and electron microscopy. Internalization occurred through a cytochalasin D-sensitive process. Invasion efficiencies of some A. actinomycetemcomitans strains were comparable to those of invasive members of the family Enterobacteriaceae. Differen...

  1. tfoX (sxy)-dependent transformation of Aggregatibacter (Actinobacillus) actinomycetemcomitans

    Bhattacharjee, Mrinal K.; Fine, Daniel H.; Figurski, David H.

    2007-01-01

    tfoX (sxy) is a regulatory gene needed to turn on competence genes. Aggregatibacter (Actinobacillus) actinomycetemcomitans has a tfoX gene that is important for transformation. We cloned this gene on an IncQ plasmid downstream of the inducible tac promoter. When this plasmid was resident in cells of A. actinomycetemcomitans and tfoX was induced, the cells became competent for transformation. Several strains of A. actinomycetemcomitans, including different serotypes, as well as rough (adherent...

  2. Purification, serology and pathogenic role of the 110 kilodalton rtx hemolysins of Actinobacillus pleuropneumoniae

    Ma, Jianneng

    1991-01-01

    Actinobacillus pleuropneumoniae is the etiological agent of contagious swine pleuropneumonia, an economically important disease of the swine industry worldwide. Improved control of this disease requires enhanced understanding of the factors contributing to pathogenesis. The objectives of this study were to investigate the immune response and virulence properties of the 110-kilodalton (110-KDa) hemolysins [hemolysin I (HlyI) and hemolysin II (HlyII)] of A. pleuropneumoniae...

  3. Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans in the Plaque of Children without Periodontitis

    W. Zimmer; Wilson, M.; Marsh, P D; Newman, H.N.; Bulman, J.

    2011-01-01

    The aim of this study was to find out whether the suspected periodontal pathogens Actinobacillus actinomycetemcomitans, Prevotella intermedia and Porphyromonas gingivalis could be recovered from the dental plaque and the dorsum of the tongue in children without periodontal breakdown. Thirty-six male Caucasian children participated in this study, 21 aged 6-11 y and 15 aged 14-16 y. Subcontact area plaque and tongue samples were cultured on selective media and a presumptive identification of th...

  4. Acquisition and Colonization Stability of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in Children

    Lamell, Celeste W.; Griffen, Ann L.; McClellan, Dawn L.; Eugene J Leys

    2000-01-01

    The presence of Porphyromonas gingivalis has been shown to be a risk factor for periodontitis in adults, and Actinobacillus actinomycetemcomitans has been implicated as a pathogen in early-onset periodontitis. Both species have been shown to establish stable colonization in adults. In cross-sectional studies, both A. actinomycetemcomitans and P. gingivalis have been detected in over one-third of apparently healthy children. Information on the stability of colonization with these organisms in ...

  5. Evaluation and application of ribotyping for epidemiological studies of Actinobacillus pleuropneumoniae in Denmark

    Fussing, V.; Barfod, Kristen; Nielsen, R.; Møller, K.; Nielsen, J.P.; Wegener, Henrik Caspar; Bisgaard, M.

    1998-01-01

    The aim of the present study was to evaluate ribotyping as an epidemiological tool for Actinobacillus pleuropneumoniae and apply the method in studies of A. pleuropneumoniae infections in Danish pig herds. The evaluation of ribotyping was based on the 13 international reference strains and 106 ep...... strains were closely related, though only showing 33% similarity to HindIII ribotypes of remaining serotypes. (C) 1998 Elsevier Science B.V. All rights reserved....

  6. Actinobacillus Actinomycetemcomitans y Porphyromonas Gingivales como principales patógenos periodontales

    A Bascones; A Caballeros

    2000-01-01

    Entre las bacterias relacionadas con la enfermedad periodontal, existen dos especies más claramente asociadas a esta enfermedad: Actinobacillus actinomycetemcomitans y Porphyromonas gingivalis. Este trabajo es una revisión bibliográfica sobre estos dos patógenos periodontales, mostrando su origen, prevalencia, distribución, transmisión y respuesta al tratamiento periodontal.Among the bacteria related to periodontal disease, there are two species clearly associated to this disease: Actinobacil...

  7. Effect of Ciprofloxacin on Killing of Actinobacillus actinomycetemcomitans by Polymorphonuclear Leukocytes

    Cacchillo, David A.; Walters, John D.

    2002-01-01

    Actinobacillus actinomycetemcomitans, a pathogen associated with aggressive periodontitis, resists phagocytic killing by polymorphonuclear leukocytes (PMNs). It is susceptible to ciprofloxacin, which PMNs actively accumulate. This study tested the hypothesis that ciprofloxacin-loaded PMNs are more effective at killing A. actinomycetemcomitans than control PMNs. Isolated human PMNs were loaded by brief incubation with 0.5 μg of ciprofloxacin/ml. Opsonized bacteria (ATCC 43718) were incubated a...

  8. Actinobacillus actinomycetemcomitans in Human Periodontal Disease: a Cross-Sectional Microbiological Investigation

    Slots, Jørgen; Reynolds, Homer S.; Genco, Robert J.

    1980-01-01

    Actinobacillus actinomycetemcomitans is a facultative gram-negative bacterium which has been associated with severe oral and nonoral infections. This study examined its occurrence in the oral cavities of 10 normal juveniles, 11 normal adults, 10 juvenile periodontitis patients, and 12 adult periodontitis patients. Four deep periodontal pockets and two normal periodontal sites were sampled in the diseased patients, and six normal periodontal sites were sampled in the healthy individuals. In al...

  9. Actinobacillus suis and Actinobacillus equuli, emergent pathogens of septic embolic nephritis, a new challenge for the swine industry Actinobacillus suis y Actinobacillus equuli, patógenos emergentes de nefritis embólica séptica, un nuevo desafío para la industria porcina

    CE Benavente

    2012-01-01

    Full Text Available Kidney lesions are an important cause of tissue condemnation in slaughterhouses. In addition to the potential public health implications, organ condemnations have a significant economic impact on the food animal industry. The condition classified broadly as "nephritis" is one of the main causes of tissue condemnation. Embolic nephritis resembling Actinobacillus equuli infection in foals has been recently detected in sows and market hogs. Actinobacillus suis is phenotypically and phylogenetically closely related to A. equuli. Both are Gram-negative bacteria, not easy to detect in routine exams. A. suis is an opportunistic pathogen that can produce fatal septicaemia in pigs, pneumonia, polyarthritis, septic embolic nephritis, abortion and mummified foetuses. Outbreaks of clinical disease appear to occur more frequently in high-health-status herds. In adult pigs the skin lesions may be confused with porcine erysipelas. A. suis and A. equuli are emerging opportunistic pathogens in the porcine industry and both have potential public health consequences to people that handles meat products. The objective of this paper is to present a literature review regarding the role of A. suis and A. equuli in the pathogenesis of nephritis in swine.Las lesiones renales son una causa importante de decomiso en los mataderos. Además de las posibles consecuencias en salud pública, el decomiso de órganos tiene un gran impacto económico en la industria de alimento animal. Recientemente, nefritis embólica séptica con lesiones semejantes a infecciones con Actinobacillus equuli en potrillos ha sido detectada en reproductoras y cerdos con peso de mercado. Actinobacillus equuli es fenotípica y genéticamente similar a Actinobacillus suis. Ambas son bacterias Gram-negativas difíciles de diagnosticar en exámenes de rutina. A. suis es un patógeno oportunista capaz de producir septicemia en cerdos, neumonía, poliartritis, nefritis embólica séptica, aborto y fetos

  10. The genetic organization of the capsular polysaccharide biosynthesis region of Actinobacillus pleuropneumoniae serotype 14

    Ito, Hiroya

    2015-01-01

    The genetic organization of the gene involved in the capsular polysaccharide (CPS) biosynthesis of Actinobacillus pleuropneumoniae serotype 14 has been determined. The DNA region for the CPS biosynthesis of serotype 14 (cps14) comprised 9 open reading frames, designated as cps14AB1B2B3CDEFG genes, encoding Cps14A to Cps14G protein, respectively. Cps14A was similar to CpsA of A. pleuropneumoniae serotypes 1, 4 and 12; the Cps14B1 and Cps14B2 were similar to CpsB of A. pleuropneumoniae serotype...

  11. The genetic organization of the capsular polysaccharide biosynthesis region of Actinobacillus pleuropneumoniae serotype 15

    Ito, Hiroya; Sueyoshi, Masuo

    2014-01-01

    Nucleotide sequence determination and analysis of the cps gene involved in the capsular polysaccharide biosynthesis of Actinobacillus pleuropneumoniae serotype 15 revealed the presence of three open reading frames, designated as cps15ABC genes. At the protein level, Cps15A and Cps15B showed considerably high homology to CpsA (67.0 to 68.7%) and CpsB (31.7 to 36.8%), respectively, of A. pleuropneumoniae serotypes 1, 4 and 12, revealing the common genetic organization of the cps among serotypes...

  12. luxS and arcB Control Aerobic Growth of Actinobacillus actinomycetemcomitans under Iron Limitation

    Fong, Karen P.; Gao, Ling; Demuth, Donald R.

    2003-01-01

    LuxS is responsible for the production of autoinducer 2 (AI-2), which functions in Vibrio harveyi as a quorum-sensing signal that controls the cell density-dependent expression of the lux operon. In nonluminescent organisms, the physiologic role of AI-2 is not clear. We report that inactivation of luxS in Actinobacillus actinomycetemcomitans JP2 results in reduced growth of the mutant, but not the wild-type organism, under aerobic, iron-limited conditions. Stunted cultures of the luxS mutant ...

  13. Characterization of two genes encoding distinct transferrin-binding proteins in different Actinobacillus pleuropneumoniae isolates.

    Gerlach, G F; Klashinsky, S; Anderson, C.; Potter, A A; Willson, P.J.

    1992-01-01

    The gene encoding the Actinobacillus pleuropneumoniae serotype 1 transferrin-binding protein (tfbA) was cloned, and the carboxy-terminal 70% of the protein was expressed as an aggregate protein in Escherichia coli. The nucleotide sequences of the tfbA genes from A. pleuropneumoniae serotypes 7 (G.-F. Gerlach, C. Anderson, A. A. Potter, S. Klashinsky, and P. J. Willson, Infect. Immun. 60:892-898, 1992) and 1 were determined, and a comparison revealed that they had 65% sequence identity. The de...

  14. Mapping of functional regions on the transferrin-binding protein (TfbA) of Actinobacillus pleuropneumoniae.

    Strutzberg, K; von Olleschik, L; Franz, B.; Pyne, C; Schmidt, M. A.; Gerlach, G F

    1995-01-01

    Actinobacillus pleuropneumoniae can use porcine transferrin as the sole source of iron. Two proteins with molecular masses of approximately 60 kDa (TfbA) and 110 kDa have been shown to specifically bind porcine transferrin; from the TfbA protein, three isoforms from A. pleuropneumoniae serotypes 1, 5, and 7 have been identified and characterized by nucleotide sequence analysis. Here we defined the transferrin-binding region(s) of the TfbA protein of A. pleuropneumoniae serotype 7 by TnphoA mu...

  15. Cloning and molecular characterization of Cu,Zn superoxide dismutase from Actinobacillus pleuropneumoniae.

    Langford, P R; Loynds, B M; Kroll, J S

    1996-01-01

    Copper-zinc superoxide dismutases (Cu,Zn SODs), until recently considered very unusual in bacteria, are now being found in a wide range of gram-negative bacterial species. Here we report the cloning and characterization of sodC, encoding Cu,Zn SOD in Actinobacillus pleuropneumoniae, a major pathogen of pigs and the causative organism of porcine pleuropneumonia. sodC was shown to lie on a monocistronic operon, at the chromosomal locus between the genes asd (encoding aspartate semialdehyde dehy...

  16. Influence of Serum and Glucose Additives on Survival of Actinobacillus pleuropneumoniae Aerosolized from the Freeze-Dried State

    Hensel, Andreas

    1994-01-01

    Serum and/or glucose added to Actinobacillus pleuropneumoniae suspensions before freeze-drying significantly increased survival rates of bacteria in aerosols. Aerosols with predictable numbers of viable bacteria can be made as required in an aerosol infection model. Sucrose supplementation of impinger fluids increased recovery of viable A. pleuropneumoniae.

  17. Production of succinic acid from oil palm empty fruit bunch cellulose using Actinobacillus succinogenes

    Pasma, Satriani Aga; Daik, Rusli; Maskat, Mohamad Yusof

    2013-11-01

    Succinic acid is a common metabolite in plants, animals and microorganisms. It has been used widely in agricultural, food and pharmaceutical industries. Enzymatic hydrolysate glucose from oil palm empty fruit bunch (OPEFB) cellulose was used as a substrate for succinic acid production using Actinobacillus succinogenes. Using cellulose extraction from OPEFB can enhance the production of glucose as a main substrate for succinic acid production. The highest concentration of glucose produced from enzymatic hydrolysis is 167 mg/mL and the sugar recovery is 0.73 g/g of OPEFB. By optimizing the culture medium for succinic acid fermentation with enzymatic hydrolysate of OPEFB cellulose, the nitrogen sources could be reduced to just only 2.5 g yeast extract and 2.5 g corn step liquor. Batch fermentation was carried out using enzymatic hydrolysate of OPEFB cellulose with yeast extract, corn steep liquor and the salts mixture, 23.5 g/L succinic acid was obtained with consumption of 72 g/L glucose in enzymatic hydrolysate of OPEFB cellulose at 38 hours and 37°C. This study suggests that enzymatic hydrolysate of OPEFB cellulose maybe an alternative substrate for the efficient production of succinic acid by Actinobacillus succinogenes.

  18. SÜT DİŞİ DİZİSİNDE ACTINOBACILLUS ACTINOMYCETEMCOMITANS'IN VARLIĞI-THE OCCURRENCE OF ACTINOBACILLUS ACTINOMYCETEMCOMITANS IN THE PRIMARY DENTITION

    Aren, Gamze; Aktören, Oya; Külekçi, Güven

    2012-01-01

    ÖzetActinobacillus actinomycetemcomitans lokalİze jüve-nil periodontitis, Papjllon- Le fevre sendromunun bazı olguları ve erişkinlerde hızlı ilerleyen periodontitiste başlıca sorumlu patojendir. Bununla birlikte normal ağız florasının da bir yerleşiği olarak bulunabilir. Bu çalışmada 4-7 yaşlan arasındaki sağlıklı 16 çocukta süt diş dizinde A.actinomycetemcomitans'ıun varbğı araştırılmıştır. Bu amaçla her çocuktan alt ikinci süt azısının mesialinden diş ipi ile plak ve dil sırtından bakteriyo...

  19. Differences in iron acquisition from human haemoglobin among strains of Actinobacillus actinomycetemcomitans

    Hayashida, H.; Poulsen, Knud; Kilian, Mogens

    2002-01-01

    other strains of the species. None of the strains examined could utilize human transferrin as a source of iron. This was in accordance with the presence of a non-functional tbpA gene, which normally encodes the A subunit of the transferrin-binding-protein complex. Southern blot analysis indicated that......To get a better insight into the physiology of the high-toxic JP2 clone of Actinobacillus actinomycetemcomitans serotype b, which is strongly associated with juvenile periodontitis in adolescents of African descent, the modes of iron acquisition in this clone were examined and compared to those of...... functional duplications of tbpA were not present in the genome. Thus, A. actinomycetemcomitans seems to be in a process of evolution, in which iron acquisition from host transferrin is not essential as in many other members of the pasteurellaceae. All strains could utilize haem as a source of iron. All 11 A...

  20. Actinobacillus pleruropneumoniae transcriptome analysis during early infection - coping with a hostile environment

    Schou, Kirstine Klitgaard; Rundsten, Carsten Friis; Jensen, Tim Kåre;

    2011-01-01

    Aim: To obtain an increased understanding of how the porcine lung pathogen Actinobacillus pleuropneumoniae (Ap) establish infection in the host. Understanding the means by which a pathogen establishes and maintains infection in the host organism is the first step towards controlling disease...... the next 42 hours. Functional analysis identified a number of putative virulence genes to be initially up-regulated. Conclusions: This is the first study monitoring the development of Ap response in the porcine host during early infection. The ability of pathogenic bacteria to adjust gene expression...... in response to environmental stimuli is critical for bacterial survival within the host. The genes identified as differentially expressed in this study may represent a core set of genes which are mobilized to cope with the host immune response and adapt to the hostile environment. The potential virulence...

  1. Succinic acid production from duckweed (Landoltia punctata) hydrolysate by batch fermentation of Actinobacillus succinogenes GXAS137.

    Shen, Naikun; Wang, Qingyan; Zhu, Jing; Qin, Yan; Liao, Siming; Li, Yi; Zhu, Qixia; Jin, Yanling; Du, Liqin; Huang, Ribo

    2016-07-01

    Duckweed is potentially an ideal succinic acid (SA) feedstock due to its high proportion of starch and low lignin content. Pretreatment methods, substrate content and nitrogen source were investigated to enhance the bioconversion of duckweed to SA and to reduce the costs of production. Results showed that acid hydrolysis was an effective pretreatment method because of its high SA yield. The optimum substrate concentration was 140g/L. The optimum substrate concentration was 140g/L. Corn steep liquor powder could be considered a feasible and inexpensive alternative to yeast extract as a nitrogen source. Approximately 57.85g/L of SA was produced when batch fermentation was conducted in a 1.3L stirred bioreactor. Therefore, inexpensive duckweed can be a promising feedstock for the economical and efficient production of SA through fermentation by Actinobacillus succinogenes GXAS137. PMID:27023386

  2. A multiplexed immunoassay for detection of antibodies to Actinobacillus pleuropneumoniae (App) in pigs

    Berger, Sanne Schou; Boas, Ulrik; Andresen, Lars Ole;

    2014-01-01

    The bacterium Actinobacillus pleuropneumoniae (App) is the causative agent of porcine pleuropneumoniae, a contagious and severe respiratory disease in pigs. Based on capsular antigens, 15 App serovars have been described, and the prevalence and morbidity of these serovars vary with geographic...... regions (1). In Denmark, the most important serovars are considered to be App 1, 2, 5, 6, 7, 10 and 12. As part of the Danish surveillance program for App, the Danish Veterinary Institute uses ELISAs and complement fixation tests (CFT) to test for porcine anti-App antibodies (2-7). In an effort to improve...... our diagnostic tools, we are currently developing a novel indirect fluorescent microsphere immunoassay that can facilitate simultaneous detection of antibodies towards multiple App serovars within a single serum sample volume. The multiplex immunoassay is based on Luminex technology (8) and has...

  3. Profiling microRNAs in lung tissue from pigs infected with Actinobacillus pleuropneumoniae

    Podolska Agnieszka

    2012-09-01

    Full Text Available Abstract Background MicroRNAs (miRNAs are a class of non-protein-coding genes that play a crucial regulatory role in mammalian development and disease. Whereas a large number of miRNAs have been annotated at the structural level during the latest years, functional annotation is sparse. Actinobacillus pleuropneumoniae (APP causes serious lung infections in pigs. Severe damage to the lungs, in many cases deadly, is caused by toxins released by the bacterium and to some degree by host mediated tissue damage. However, understanding of the role of microRNAs in the course of this infectious disease in porcine is still very limited. Results In this study, the RNA extracted from visually unaffected and necrotic tissue from pigs infected with Actinobacillus pleuropneumoniae was subjected to small RNA deep sequencing. We identified 169 conserved and 11 candidate novel microRNAs in the pig. Of these, 17 were significantly up-regulated in the necrotic sample and 12 were down-regulated. The expression analysis of a number of candidates revealed microRNAs of potential importance in the innate immune response. MiR-155, a known key player in inflammation, was found expressed in both samples. Moreover, miR-664-5p, miR-451 and miR-15a appear as very promising candidates for microRNAs involved in response to pathogen infection. Conclusions This is the first study revealing significant differences in composition and expression profiles of miRNAs in lungs infected with a bacterial pathogen. Our results extend annotation of microRNA in pig and provide insight into the role of a number of microRNAs in regulation of bacteria induced immune and inflammatory response in porcine lung.

  4. Inokulasi Bakteri Selulolitik Actinobacillus sp. Asal Rumen pada Daun Jati Menurunkan Serat Kasar dan Meningkatkan Protein Kasar

    Mirni Lamid; Anggun Foetus Eka Julita; Ngakan Made Rai Widjaya

    2013-01-01

    Constraints use teak leaves as ruminant feed is a high content of crude fiber and low crude protein.The objective of this research was to determine potency of inoculation  Actinobacillus sp. of rumen productionon teak leaves fermentation process could improve quality of teak leaves as animal feed for ruminants.Design study was Completely Randomized Design with four treatments and five replications. Fourtreatment groups were consist of  : P0 = teak leaves + 2% molasses (control); P1 = teak lea...

  5. Comparison of conventional and long-acting oxytetracyclines in prevention of induced Actinobacillus (Haemophilus) pleuropneumoniae infection of growing swine.

    Kiorpes, A L; Bäckström, L R; Collins, M T; Kruse, G O

    1989-01-01

    These experiments tested the hypothesis that long-acting oxytetracycline (oxytetracycline-LA) was more effective than regular oxytetracycline in preventing porcine pleuropneumonia when administered either 24 or 48 h prior to experimental challenge with virulent strains of Actinobacillus pleuropneumoniae. Two experiments (1 and 2) were conducted using growing pigs (average weight 12-15 kg). Antibiotic treatments were administered once intramuscularly at 20 mg/kg body weight; controls received ...

  6. Differential expression of the cytotoxic and hemolytic activities of the ApxIIA toxin from Actinobacillus pleuropneumoniae.

    Tu, A H; Hausler, C; Young, R.; Struck, D K

    1994-01-01

    The ApxIIA protein secreted from Actinobacillus pleuropneumoniae is both hemolytic and cytotoxic. However, when the cloned apxII operon is expressed in Escherichia coli, two forms of the ApxIIA protein can be recovered. Toxin which remains intracellular has hemolytic and cytotoxic activities, while toxin that is secreted is cytotoxic with little or no hemolytic activity. This indicates that the cytotoxicity of ApxIIA is independent of its hemolytic activity.

  7. Relationship Between Conversion of Localized Juvenile Periodontitis-Susceptible Children From Health to Disease and Actinobacillus actinomycetemcomitans Leukotoxin Promoter Structure*

    Bueno, Lina C.; Mayer, Marcia P. A.; DiRienzo, Joseph M.

    1998-01-01

    The periodontal pathogen Actinobacillus actinomycetemcomitans produces a leukotoxin that is considered a primary virulence factor in localized juvenile Periodontitis (LJP). Select strains of the bacterium contain a 530-bp deletion in the promoter region of the leukotoxin gene operon which results in enhanced transcription of the leukotoxin. DNA hybridization and polymerase chain reaction (PCR) were used to examine genetic variants of A. actinomycetemcomitans in 24 LJP-susceptible children fro...

  8. GENETIC APPROACH TO THE STUDY OF EPIDEMIOLOGY AND PATHOGENESIS OF ACTINOBACILLUS ACTINOMYCETEMCOA4ITANS IN LOCALIZED JUVENILE PERIODONTITIS

    DiRienzo, J M; Slots, J

    1990-01-01

    Actinobacillus acrinomycetemcomirans isolates from periodontal pockets were examined for restriction fragment-length polymorphism using a characterized 4.7-kb DNA probe. A total of 6 patterns of RFLP was found in 133 isolates originating from 12 subjects. No relatedness was found between RFLP types and serotypes. Different periodontal sites within the same subject and different individuals within the same family sometimes showed only one type of A. actinomycetemcomitans RFLP. When members amo...

  9. Interference of peptides and specific antibodies with the function of the Actinobacillus pleuropneumoniae transferrin-binding protein.

    Strutzberg, K; Franz, B.; Gerlach, G F

    1997-01-01

    Multiple-antigenic peptides (MAPs) containing transferrin-binding domains of the Actinobacillus pleuropneumoniae serotype 7-derived transferrin-binding protein (TfbA) (K. Strutzberg, L. von Olleschik, B. Franz, C. Pyne, M. A. Schmidt, and G.-F. Gerlach, Infect. Immun. 63:3846-3850, 1995) were constructed. It was found that the MAPs inhibited transferrin binding of the recombinant TfbA protein, whereas antibodies directed against transferrin-binding domains failed to do so.

  10. Use of 16S rRNA Sequencing for Identification of Actinobacillus ureae Isolated from a Cerebrospinal Fluid Sample

    Whitelaw, A. C.; Shankland, I. M.; Elisha, B. G.

    2002-01-01

    Actinobacillus ureae, previously Pasteurella ureae, has on rare occasions been described as a cause of human infection. Owing to its rarity, it may not be easily identified in clinical microbiology laboratories by standard tests. This report describes a patient with acute bacterial meningitis due to A. ureae. The identity of the isolate was determined by means of DNA sequence analysis of a portion of the 16S rRNA gene.

  11. Significance of CO2 donor on the production of succinic acid by Actinobacillus succinogenes ATCC 55618

    Zou Wei

    2011-10-01

    Full Text Available Abstract Background Succinic acid is a building-block chemical which could be used as the precursor of many industrial products. The dissolved CO2 concentration in the fermentation broth could strongly regulate the metabolic flux of carbon and the activity of phosphoenolpyruvate (PEP carboxykinase, which are the important committed steps for the biosynthesis of succinic acid by Actinobacillus succinogenes. Previous reports showed that succinic acid production could be promoted by regulating the supply of CO2 donor in the fermentation broth. Therefore, the effects of dissolved CO2 concentration and MgCO3 on the fermentation process should be investigated. In this article, we studied the impacts of gaseous CO2 partial pressure, dissolved CO2 concentration, and the addition amount of MgCO3 on succinic acid production by Actinobacillus succinogenes ATCC 55618. We also demonstrated that gaseous CO2 could be removed when MgCO3 was fully supplied. Results An effective CO2 quantitative mathematical model was developed to calculate the dissolved CO2 concentration in the fermentation broth. The highest succinic acid production of 61.92 g/L was obtained at 159.22 mM dissolved CO2 concentration, which was supplied by 40 g/L MgCO3 at the CO2 partial pressure of 101.33 kPa. When MgCO3 was used as the only CO2 donor, a maximal succinic acid production of 56.1 g/L was obtained, which was just decreased by 7.03% compared with that obtained under the supply of gaseous CO2 and MgCO3. Conclusions Besides the high dissolved CO2 concentration, the excessive addition of MgCO3 was beneficial to promote the succinic acid synthesis. This was the first report investigating the replaceable of gaseous CO2 in the fermentation of succinic acid. The results obtained in this study may be useful for reducing the cost of succinic acid fermentation process.

  12. Transcriptional Profiling of Hilar Nodes from Pigs after Experimental Infection with Actinobacillus Pleuropneumoniae

    Shumin Yu

    2013-11-01

    Full Text Available The gram-negative bacterium Actinobacillus pleuropneumoniae (APP is an inhabitant of the porcine upper respiratory tract and the causative agent of porcine pleuropneumonia (PP. In recent years, knowledge about the proinflammatory cytokine and chemokine gene expression that occurs in lung and lymph node of the APP-infected swine has been advanced. However, systematic gene expression profiles on hilar nodes from pigs after infection with Actinobacillus pleuropneumoniae have not yet been reported. The transcriptional responses were studied in hilar nodes (HN from swine experimentally infected with APP and the control groupusing Agilent Porcine Genechip, including 43,603 probe sets. 9,517 transcripts were identified as differentially expressed (DE at the p ≤ 0.01 level by comparing the log2 (normalized signal of the two groups named treatment group (TG and controls (CG. Eight hundred and fifteen of these DE transcripts were annotated as pig genes in the GenBank database (DB. Two hundred and seventy-two biological process categories (BP, 75 cellular components and 171 molecular functions were substantially altered in the TG compared to CG. Many BP were involved in host immune responses (i.e., signaling, signal transmission, signal transduction, response to stimulus, oxidation reduction, response to stress, immune system process, signaling pathway, immune response, cell surface receptor linked signaling pathway. Seven DE gene pathways (VEGF signaling pathway, Long-term potentiation, Ribosome, Asthma, Allograft rejection, Type I diabetes mellitus and Cardiac muscle contraction and statistically significant associations with host responses were affected. Many cytokines (including NRAS, PI3K, MAPK14, CaM, HSP27, protein phosphatase 3, catalytic subunit and alpha isoform, mediating the proliferation and migration of endothelial cells and promoting survival and vascular permeability, were activated in TG, whilst many immunomodulatory cytokines were

  13. Rapid detection of Actinobacillus actinomycetemcomitans, Prevotella intermedia and Porphyromona gingivalis by multiplex PCR.

    García, L; Tercero, J C; Legido, B; Ramos, J A; Alemany, J; Sanz, M

    1998-01-01

    The identification of specific periodontal pathogens by conventional methods, mainly anaerobic cultivation, is difficult, time consuming and even sometimes unreliable. Therefore, a multiplex PCR method for simultaneous detection of Actinobacillus actinomycetemcomitans (A.a.), Porphyromona gingivalis (P.g.) and Prevotella intermedia (P.i.) was developed for rapid and easy identification of these specific bacterial pathogens in subgingival plaque samples. In this paper, there is a detailed description of the oligonucleotide primer selection, DNA extraction and PCR conditions and the sequencing of the amplified products. The locus chosen to be amplified is a highly variable region in the 16S ribosomal DNA. For the development of this technique ATCC cultures and pure cultures from subgingival plaque samples taken from periodontitis patients were used. As an internal positive control a recombinant plasmid was developed. This simple DNA extraction procedure and the DNA amplification and visualization of the amplified product permits the detection of the bacteria in a working day. Thus, this multiplex PCR method is a rapid and effective detection method for specific periodontal pathogens. PMID:9524322

  14. A complete industrial system for economical succinic acid production by Actinobacillus succinogenes.

    Li, Jian; Zheng, Xiao-Yu; Fang, Xiao-Jiang; Liu, Shu-Wen; Chen, Ke-Quan; Jiang, Min; Wei, Ping; Ouyang, Ping-Kai

    2011-05-01

    An industrial fermentation system using lignocellulosic hydrolysate, waste yeast hydrolysate, and mixed alkali to achieve high-yield, economical succinic acid production by Actinobacillus succinogenes was developed. Lignocellulosic hydrolysate and waste yeast hydrolysate were used efficiently as carbon sources and nitrogen source instead of the expensive glucose and yeast extract. Moreover, as a novel method for regulating pH mixed alkalis (Mg(OH)(2) and NaOH) were first used to replace the expensive MgCO(3) for succinic acid production. Using the three aforementioned substitutions, the total fermentation cost decreased by 55.9%, and 56.4 g/L succinic acid with yield of 0.73 g/g was obtained, which are almost the same production level as fermentation with glucose, yeast extract and MgCO(3). Therefore, the cheap carbon and nitrogen sources, as well as the mixed alkaline neutralize could be efficiently used instead of expensive composition for industrial succinic acid production. PMID:21470857

  15. Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China.

    Zhuofei Xu

    Full Text Available Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, a cause of considerable world wide economic losses in the swine industry. We sequenced the complete genome of A. pleuropneumoniae, JL03, an isolate of serotype 3 prevalent in China. Its genome is a single chromosome of 2,242,062 base pairs containing 2,097 predicted protein-coding sequences, six ribosomal rRNA operons, and 63 tRNA genes. Preliminary analysis of the genomic sequence and the functions of the encoded proteins not only confirmed the present physiological and pathological knowledge but also offered new insights into the metabolic and virulence characteristics of this important pathogen. We identified a full spectrum of genes related to its characteristic chemoheterotrophic catabolism of fermentation and respiration with an incomplete TCA system for anabolism. In addition to confirming the lack of ApxI toxin, identification of a nonsense mutation in apxIVA and a 5'-proximal truncation of the flp operon deleting both its promoter and the flp1flp2tadV genes have provided convincing scenarios for the low virulence property of JL03. Comparative genomic analysis using the available sequences of other serotypes, probable strain (serotype-specific genomic islands related to capsular polysaccharides and lipopolysaccharide O-antigen biosyntheses were identified in JL03, which provides a foundation for future research into the mechanisms of serotypic diversity of A. pleuropneumoniae.

  16. Characterization of an Actinobacillus pleuropneumoniae seeder pig challenge-exposure model.

    Lechtenberg, K F; Shryock, T R; Moore, G

    1994-12-01

    Five strains of Actinobacillus pleuropneumoniae serotype 1 were used to intranasally infect 5 groups of pigs. Using each bacterial strain, infected pigs (termed seeder pigs) were commingled for 48 hours with 5 groups of noninfected test pigs, then were removed. Seeder and test pigs were maintained in isolation and were observed for 14 days. Seeder pigs had mortality that was threefold greater than that of test pigs (24% vs 8%). Rectal temperature in excess of 40.3 C was achieved for 84% of test pigs and 88% of seeder pigs. Neither of these 2 variables was statistically different between the 2 groups of pigs. Clinical impression scores > or = 2 (on a 0 to 3 scale) were three-fold (64% vs 20%) greater for seeder than for test pigs (P amount of lesions. The number of A pleuropneumoniae isolations was not statistically different between test and seeder pig populations. Recovery of Pasteurella multocida or other bacteria was greater from the seeder pigs (P recoverable isolates was greater from test pigs than from seeder pigs (P < 0.05). Assessment of lung lesions at necropsy by either visual estimation or on a weight basis were in agreement.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:7887514

  17. Possible involvement of protein kinase C in apoptotic cell death of macrophages infected with Actinobacillus actinomycetemcomitans.

    Nonaka, K; Ishisaki, A; Muro, M; Kato, S; Oido, M; Nakashima, K; Kowashi, Y; Nishihara, T

    1998-02-15

    We have previously reported the evidence for apoptosis in the mouse macrophage cell line J774.1 by the periodontopathic bacterium Actinobacillus actinomycetemcomitans. In this study, we examined the role of protein kinases in the induction of apoptosis in A. actinomycetemcomitans-infected J774.1 cells by the MTT assay, fluorescence microscopy and flow cytometric analysis. After J774.1 cells were precultured with protein kinase C (PKC) activator, phorbol 12-myristate 13-acetate (PMA), J774.1 cells infected with A. actinomycetemcomitans showed the increased percentage of apoptotic cells. On the contrary, protein kinase A (PKA) activators, such as forskolin and dibutyryl cAMP, do not mimic the effect of PMA. PKC inhibitors, such as staurosporine, calphostin C, chelerythrine chloride, and H7 were found to suppress apoptotic cell death in J774.1 cells infected with A. actinomycetemcomitans. However, HA1004, known as PKA inhibitor, had no effect on apoptosis in infected macrophages. The results presented here suggest that the signals through PKC may play crucial roles in the modulation of apoptosis in macrophages infected with A. actinomycetemcomitans. PMID:9503618

  18. Antioxidant effect of minocycline in gingival epithelium induced by Actinobacillus actinomycetemcomitans serotype B toxin

    Ernie Maduratna Setiawati

    2009-03-01

    Full Text Available Background: Actinobacillus actinomycetemcomitans (Aa serotype B has been associated with aggressive periodontitis. Gingival epithelial cell is exquisitely sensitive to the toxin and may lead to the epithel protective barrier disruption. Experimental models show that minocycline is not related to it’s antimicrobial effect and protection against neuron cell apoptosis of a number experimental models of brain injury and Parkinson’s disease. Purpose: This study, examined antioxidant effect of minocycline to inhibit apoptosis of gingival epithelium induced crude toxin bacteria Aa serotype B in mice. Methods: Thirty adult mice strain Swiss Webster (balb C were divided randomly into three groups: control group (group A, toxin group (group B and toxin and minocycline group (group C. The mice were taken at 24 hours after application, and then the tissue sections of gingival epithelium were stained with tunnel assay and immunohistochemistry. Result: Treatment with these toxin induced apoptosis of gingival epithelium and was associated with DNA fragmentation and reduced gluthatione (GSH. Minocycline 100 nM significantly increased GSH and reduced apoptosis (p < 0.05. Minocycline provides antioxidant effect against citotoxicity of bacteria Aa serotipe B. Conclusion: Nanomolar concentration of minocycline potential as new therapeutic agent to prevent progressivity of aggressiveness of periodontitis.

  19. Optimization of succinic acid fermentation with Actinobacillus succinogenes by response surface methodology (RSM)

    Yun-jian ZHANG; Qiang LI; Yu-xiu ZHANG; Dan WANG; Jian-min XING

    2012-01-01

    Succinic acid is considered as an important platform chemical.Succinic acid fermentation with Actinobacillus succinogenes strain BE-1 was optimized by central composite design (CCD) using a response surface methodology (RSM).The optimized production of succinic acid was predicted and the interactive effects between glucose,yeast extract,and magnesium carbonate were investigated.As a result,a model for predicting the concentration of succinic acid production was developed.The accuracy of the model was confirmed by the analysis of variance (ANOVA),and the validity was further proved by verification experiments showing that percentage errors between actual and predicted values varied from 3.02% to 6.38%.In addition,it was observed that the interactive effect between yeast extract and magnesium carbonate was statistically significant.In conclusion,RSM is an effective and useful method for optimizing the medium components and investigating the interactive effects,and can provide valuable information for succinic acid scale-up fermentation using A.succinogenes strain BE-1.

  20. Detection of highly and minimally leukotoxic Actinobacillus actinomycetemcomitans strains in patients with periodontal disease

    Cortelli Sheila Cavalca

    2003-01-01

    Full Text Available This study examined the prevalence of highly and minimally leukotoxic Actinobacillus actinomycetemcomitans in patients with periodontal disease. Pooled subgingival plaque samples from 136 patients with some form of periodontal disease were examined. Subjects were between 14 and 76 years of age. Clinical examinations included periodontal pocket depth (PD, plaque index (PI and bleeding index (BI. The obtained plaque samples were examined for the presence of highly or minimally leukotoxic A. actinomycetemcomitans strains by the polymerase chain reaction (PCR. Chi-square and logistic regression were performed to evaluate the results. Forty-seven subjects were diagnosed with gingivitis, 70 with chronic periodontitis and 19 with aggressive periodontitis. According to chi-square there was no significant correlation detected between PD (chi2 = 0.73, PI (chi2 = 0.35, BI (chi2 = 0.09 and the presence of the highly leukotoxic A. actinomycetemcomitans. The highly leukotoxic A. actinomycetemcomitans strains were correlated with subjects that were 28 years of age and younger (chi2 = 7.41. There was a significant correlation between highly leukotoxic A. actinomycetemcomitans and aggressive periodontitis (chi2 = 22.06. This study of a Brazilian cohort confirms the strong association between highly leukotoxic A. actinomycetemcomitans strains and the presence of aggressive periodontitis.

  1. Effects of Actinobacillus pleuropneumoniae cytotoxins on generation of oxygen radicals by porcine neutrophils

    Simson Tarigan

    1999-03-01

    Full Text Available Cytotoxins produced by Actinobacillus pleuropneumoniae (App suggested to be the most important pathogenic and virulent factors for this organism. However, the mechanisms on how the cytotoxins contribute to the disease process remain unclear. The purpose of this study is to investigate the effect of the cytotoxins on the oxidative-burst metabolism of porcine neutrophils. In this study, neutrophils were firstly loaded with an oxidative probe dichlorofluorescin diacetate (DCFHDA then expose to cytotoxins. Cells producing oxygen radicals emitted fluorescence and its intensity was measured with a FACScan flow cytometer. All cytotoxins derived from either App serotypes producing ApxI and ApxII, App serotypes producing ApxII only, or App serotypes producing ApxII and ApxIII were capable of stimulating neutrophils for oxygen-radical generation. However, compared with phorbol myristate acetate (PMA, App cytotoxins were much weaker as stimulants for oxygen radicals. In addition, Apx preparation stimulated an oxidative-burst metabolism of neutrophils at a low, narrow range of Apx doses. At higher doses, the toxins inhibit the oxidative burst metabolism. The effects of cytotoxins produced by App during infection on recruited neutrophils into the lungs are assumed to be comparable to those observed in this in vitro study. Neutrophils, and other host cells, adjacent to the bacteria become lysis due to high toxin concentration, whereas those at some distance to the bacteria produce oxygen radicals which in turn cause tissue damage or necrosis.

  2. New insights on the treatment of respiratory diseases caused by actinobacillus pleuropneumoniae and Haemophilus parasuis in pigs with marbofloxacin

    Vilalta Sans, Carles

    2014-01-01

    La marbofloxacina (MB) és una fluoroquinolona de tercera generació àmpliament usada en diferents espècies per tractar sobretot infeccions respiratòries. Aquest antibiòtic posseeix un ampli espectre d’activitat que inclou dos dels principals patògens associats al complexe respiratori porcí (CRP), Actinobacillus pleuropneumoniae (APP) i Haemophilus parasuis (HP). APP és l’agent etiològic de la pleuropneumònia porcina i pot romandre a les tonsil·les dels porcs sense mostrar cap mena de símptoma ...

  3. New insights on the treatment of respiratory diseases caused by actinobacillus pleuropneumoniae and Haemophilus parasuis in pigs with marbofloxacin

    Vilalta Sans, Carles; Cristòfol Adell, Carles

    2015-01-01

    La marbofloxacina (MB) és una fluoroquinolona de tercera generació àmpliament usada en diferents espècies per tractar sobretot infeccions respiratòries. Aquest antibiòtic posseeix un ampli espectre d'activitat que inclou dos dels principals patògens associats al complexe respiratori porcí (CRP), Actinobacillus pleuropneumoniae (APP) i Haemophilus parasuis (HP). APP és l'agent etiològic de la pleuropneumònia porcina i pot romandre a les tonsil·les dels porcs sense mostrar cap mena de símptoma ...

  4. Evaluation of an enzyme-linked immunosorbent assay for serological surveillance of infection with Actinobacillus pleuropneumoniae serotype 5 in pig herds

    Klausen, Joan; Andresen, Lars Ole; Barfod, Kristen; Sørensen, Vibeke

    2002-01-01

    An indirect enzyme-linked immunoassay for serological surveillance of infection of pigs with Actinobacillus pleuropneumoniae (Ap) serotype 5 was developed. The antigen used was prepared from Ap serotype 5b strain L20. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis...

  5. Real-time quantitative reverse transcription-PCR analysis of expression stability of Actinobacillus pleuropneumoniae housekeeping genes during in vitro growth under iron-depleted conditions

    Nielsen, K. K.; Boye, Mette

    2005-01-01

    The aims of the present investigation were to develop and test a sensitive and reproducible method for the study of gene expression in the porcine lung pathogen Actinobacillus pleuropneumoniae by real-time quantitative reverse transcription (RT)-PCR and to evaluate a number of suitable internal c...

  6. Evaluation of an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the Apx toxins of Actinobacillus pleuropneumoniae

    Nielsen, Ragnhild; van den Bosch, Johannes F.; Plambeck, Tamara; Sørensen, Vibeke; Nielsen, Jens Peter

    The reference strains of the 12 serotypes of Actinobacillus pleuropneumoniae express one or two of three different RTX exotoxins designated Apr I, Apr II and Apr III. The toxins are important virulence factors. In the present study, ELISAs with purified Apr I, Apr II and Apr III, respectively, as...

  7. Nitric oxide production by murine spleen cells stimulated with lipopolysaccharide from Actinobacillus actinomycetemcomitans.

    Sosroseno, Wihaskoro; Herminajeng, Endang; Susilowati, Heni; Budiarti, Sri

    2002-12-01

    The aim of this study was to determine whether Actinobacillus actinomycetemcomitans lipopolysaccharide (LPS-A. actinomycetemcomitans) could induce murine spleen cells to produce nitric oxide (NO). Spleen cells derived from Balb/c mice were stimulated with LPS-A. actinomycetemcomitans or LPS from Escherichia coli for 4 days. The effects of N(G)-monomethyl-L-arginine (NMMA), polymyxin B, and cytokines (IFN-gamma and IL-4) on the production of NO were also assessed. The NO production from the carrageenan-treated spleen cells stimulated with LPS-A. actinomycetemcomitans or both LPS-A. actinomycetemcomitans and IFN-gamma was determined. The carrageenan-treated mice were transferred with splenic macrophages and the NO production was assessed from the spleen cells stimulated with LPS-A. actinomycetemcomitans or LPS-A. actinomycetemcomitans and IFN-gamma. The results showed that NO production was detectable in the cultures of spleen cells stimulated with LPS-A. actinomycetemcomitans in a dose-dependent fashion, but was lower than in the cells stimulated with LPS from E. coli. The NO production was blocked by NMMA and polymyxin B. IFN-gamma up-regulated but IL-4 suppressed the production of NO by the spleen cells stimulated with LPS-A. actinomycetemcomitans. The carrageenan-treated spleen cells failed to produce NO after stimulation with LPS-A. actinomycetemcomitans or both LPS-A. actinomycetemcomitans and IFN-gamma. Adoptive transfer of splenic macrophages to the carrageenan-treated mice could restore the ability of the spleen cells to produce NO. The results of the present study suggest that LPS-A. actinomycetemcomitans under the regulatory control of cytokines induces murine spleen cells to produce NO and that splenic macrophages are the cellular source of the NO production. Therefore, these results may support the view that NO production by LPS-A. actinomycetemcomitans-stimulated macrophages may play a role in the course of periodontal diseases. PMID:16887678

  8. A computational strategy for the search of regulatory small RNAs in Actinobacillus pleuropneumoniae

    Rossi, Ciro C.; Bossé, Janine T.; Li, Yanwen; Witney, Adam A.; Gould, Kate A.; Langford, Paul R.; Bazzolli, Denise M.S.

    2016-01-01

    Bacterial regulatory small RNAs (sRNAs) play important roles in gene regulation and are frequently connected to the expression of virulence factors in diverse bacteria. Only a few sRNAs have been described for Pasteurellaceae pathogens and no in-depth analysis of sRNAs has been described for Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, responsible for considerable losses in the swine industry. To search for sRNAs in A. pleuropneumoniae, we developed a strategy for the computational analysis of the bacterial genome by using four algorithms with different approaches, followed by experimental validation. The coding strand and expression of 17 out of 23 RNA candidates were confirmed by Northern blotting, RT-PCR, and RNA sequencing. Among them, two are likely riboswitches, three are housekeeping regulatory RNAs, two are the widely studied GcvB and 6S sRNAs, and 10 are putative novel trans-acting sRNAs, never before described for any bacteria. The latter group has several potential mRNA targets, many of which are involved with virulence, stress resistance, or metabolism, and connect the sRNAs in a complex gene regulatory network. The sRNAs identified are well conserved among the Pasteurellaceae that are evolutionarily closer to A. pleuropneumoniae and/or share the same host. Our results show that the combination of newly developed computational programs can be successfully utilized for the discovery of novel sRNAs and indicate an intricate system of gene regulation through sRNAs in A. pleuropneumoniae and in other Pasteurellaceae, thus providing clues for novel aspects of virulence that will be explored in further studies. PMID:27402897

  9. Pharmacokinetics of tulathromycin in edible tissues of healthy and experimentally infected pigs with Actinobacillus pleuropneumoniae.

    Bladek, Tomasz; Posyniak, Andrzej; Jablonski, Artur; Gajda, Anna

    2015-01-01

    The aim of this study was the comparison of the tissue pharmacokinetics of tulathromycin in healthy pigs and pigs experimentally infected with Actinobacillus pleuropneumoniae (App). Tulathromycin was given to 24 healthy and 24 infected pigs by intramuscular injection at a single dosage of 2.5 mg kg(-1) body weight (b.w.). Pigs were euthanised at each group and then samples of liver, kidney, muscle, injection site and skin with fat were taken at scheduled time points. Drug concentrations were determined by LC-MS/MS. In this study, higher values of the area under the concentration-time curves (AUC) were calculated in all tissue samples taken from infected than healthy pigs. In pigs with App the AUCs of liver, kidney, muscle, skin with fat and injection site were 1111, 1973, 235, 181 and 2931 mg kg(-1) h, while in pigs without inflammation they were 509, 1295, 151, 111 and 1587 mg kg(-1) h, respectively. Maximum drug tissue concentrations (Cmax) in infected animals were 2370, 6650, 2016, 666 and 83,870 µg kg(-1), while in healthy pigs they were 1483, 6677, 1733, 509 and 55,006 µg kg(-1), respectively. The eliminations half-times (T1/2) were respectively longer in all tissue samples taken from infected animals (from 157.3 to 187.3 h) than in healthy ones (from 138.6 to 161.2 h). The tulathromycin tissue concentrations were significantly higher (p < 0.05) in all tissue samples of the infected pigs compared with the healthy animals at 360 h (from 0.0014 to 0.0280) and at 792 h (from 0.0007 to 0.0242) after drug administration. The results suggest that the tissue pharmacokinetic properties and residue depletion of tulathromycin can be influenced by the disease state of animals. PMID:26247868

  10. Proteomic and immunoproteomic characterization of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae

    Maas Alexander

    2011-04-01

    Full Text Available Abstract Background Protection of pigs by vaccination against Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, is hampered by the presence of 15 different serotypes. A DIVA subunit vaccine comprised of detergent-released proteins from A. pleuropneumoniae serotypes 1, 2 and 5 has been developed and shown to protect pigs from clinical symptoms upon homologous and heterologous challenge. This vaccine has not been characterized in-depth so far. Thus we performed i mass spectrometry in order to identify the exact protein content of the vaccine and ii cross-serotype 2-D immunoblotting in order to discover cross-reactive antigens. By these approaches we expected to gain results enabling us to argue about the reasons for the efficacy of the analyzed vaccine. Results We identified 75 different proteins in the vaccine. Using the PSORTb algorithm these proteins were classified according to their cellular localization. Highly enriched proteins are outer membrane-associated lipoproteins like OmlA and TbpB, integral outer membrane proteins like FrpB, TbpA, OmpA1, OmpA2, HgbA and OmpP2, and secreted Apx toxins. The subunit vaccine also contained large amounts of the ApxIVA toxin so far thought to be expressed only during infection. Applying two-dimensional difference gel electrophoresis (2-D DIGE we showed different isoforms and variations in expression levels of several proteins among the strains used for vaccine production. For detection of cross-reactive antigens we used detergent released proteins of serotype 7. Sera of pigs vaccinated with the detergent-released proteins of serotypes 1, 2, and 5 detected seven different proteins of serotype 7, and convalescent sera of pigs surviving experimental infection with serotype 7 reacted with 13 different proteins of the detergent-released proteins of A. pleuropneumoniae serotypes 1, 2, and 5. Conclusions A detergent extraction-based subunit vaccine of A. pleuropneumoniae was

  11. The role of Actinobacillus actinomycetemcomitans fimbrial adhesin on MMP-8 activity in aggressive periodontitis pathogenesis

    Rini Devijanti Ridwan

    2012-12-01

    Full Text Available Background: Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans is Gram negative and a major bacterial agent associated with aggressive periodontitis in young adult, this bacteria was an important factor in pathogenesis of aggressive periodontitis. A. actinomycetemcomitans possesses fimbriae with an adhesin protein that was the first bacterial molecules to make physical contact with host. Purpose: The objective of this research was to analyzed the influence of A. actinomycetemcomitans fimbrial adhesin protein induction on MMP-8 activity. Methods: The research was an experimental laboratory study, the step in this study were isolation and identification A. actinomycetemcomitans, characterize A. actinomycetemcomitans adhesin and study the role of A. actinomycetemcomitans adhesin in Wistar rats. Results: The result of this research on the role of adhesin in Wistar rats after analysis with Analysis of Variance (ANOVA showed significant differences in the control group with group induction with A. actinomycetemcomitans, A. actinomycetemcomitans plus adhesin and adhesin. MMP-8 activity increased with induction A. actinomycetemcomitans and 24 kDa A. actinomycetemcomitans adhesin. This fimbrial adhesin protein showed that A. actinomycetemcomitans has the ability to adhesion, colonization and invasion for host in aggressive periodontitis pathogenesis. Conclusion: A. actinomycetemcomitans fimbrial adhesin protein induction increasing MMP-8 activity for aggressive periodontitis pathogenesis.Latar belakang: A. actinomycetemcomitans merupakan salah satu bakteri Gram negatif yang terkait dengan periodontitis agresif yang menyerang penderita usia muda dan merupakan faktor penting dalam patogenesis periodontitis agresif. A. actimycetemcomitans mempunyai fimbriae dengan protein adhesin yang merupakan molekul pertama dari bakteri untuk melakukan kontak fisik dengan host. Tujuan: Tujuan penelitian ini adalah menganalisis pengaruh induksi adhesin A

  12. Experimental Actinobacillus pleuropneumoniae challenge in swine: Comparison of computed tomographic and radiographic findings during disease

    Brauer Carsten

    2012-04-01

    Full Text Available Abstract Background In pigs, diseases of the respiratory tract like pleuropneumonia due to Actinobacillus pleuropneumoniae (App infection have led to high economic losses for decades. Further research on disease pathogenesis, pathogen-host-interactions and new prophylactic and therapeutic approaches are needed. In most studies, a large number of experimental animals are required to assess lung alterations at different stages of the disease. In order to reduce the required number of animals but nevertheless gather information on the nature and extent of lung alterations in living pigs, a computed tomographic scoring system for quantifying gross pathological findings was developed. In this study, five healthy pigs served as control animals while 24 pigs were infected with App, the causative agent of pleuropneumonia in pigs, in an established model for respiratory tract disease. Results Computed tomographic (CT findings during the course of App challenge were verified by radiological imaging, clinical, serological, gross pathology and histological examinations. Findings from clinical examinations and both CT and radiological imaging, were recorded on day 7 and day 21 after challenge. Clinical signs after experimental App challenge were indicative of acute to chronic disease. Lung CT findings of infected pigs comprised ground-glass opacities and consolidation. On day 7 and 21 the clinical scores significantly correlated with the scores of both imaging techniques. At day 21, significant correlations were found between clinical scores, CT scores and lung lesion scores. In 19 out of 22 challenged pigs the determined disease grades (not affected, slightly affected, moderately affected, severely affected from CT and gross pathological examination were in accordance. Disease classification by radiography and gross pathology agreed in 11 out of 24 pigs. Conclusions High-resolution, high-contrast CT examination with no overlapping of organs is superior to

  13. A Transcriptome Map of Actinobacillus pleuropneumoniae at Single-Nucleotide Resolution Using Deep RNA-Seq

    Su, Zhipeng; Zhu, Jiawen; Xu, Zhuofei; Xiao, Ran; Zhou, Rui; Li, Lu; Chen, Huanchun

    2016-01-01

    Actinobacillus pleuropneumoniae is the pathogen of porcine contagious pleuropneumoniae, a highly contagious respiratory disease of swine. Although the genome of A. pleuropneumoniae was sequenced several years ago, limited information is available on the genome-wide transcriptional analysis to accurately annotate the gene structures and regulatory elements. High-throughput RNA sequencing (RNA-seq) has been applied to study the transcriptional landscape of bacteria, which can efficiently and accurately identify gene expression regions and unknown transcriptional units, especially small non-coding RNAs (sRNAs), UTRs and regulatory regions. The aim of this study is to comprehensively analyze the transcriptome of A. pleuropneumoniae by RNA-seq in order to improve the existing genome annotation and promote our understanding of A. pleuropneumoniae gene structures and RNA-based regulation. In this study, we utilized RNA-seq to construct a single nucleotide resolution transcriptome map of A. pleuropneumoniae. More than 3.8 million high-quality reads (average length ~90 bp) from a cDNA library were generated and aligned to the reference genome. We identified 32 open reading frames encoding novel proteins that were mis-annotated in the previous genome annotations. The start sites for 35 genes based on the current genome annotation were corrected. Furthermore, 51 sRNAs in the A. pleuropneumoniae genome were discovered, of which 40 sRNAs were never reported in previous studies. The transcriptome map also enabled visualization of 5'- and 3'-UTR regions, in which contained 11 sRNAs. In addition, 351 operons covering 1230 genes throughout the whole genome were identified. The RNA-Seq based transcriptome map validated annotated genes and corrected annotations of open reading frames in the genome, and led to the identification of many functional elements (e.g. regions encoding novel proteins, non-coding sRNAs and operon structures). The transcriptional units described in this study

  14. Transcriptional profiling of Actinobacillus pleuropneumoniae during the acute phase of a natural infection in pigs

    Harel Josée

    2010-02-01

    Full Text Available Abstract Background Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, a respiratory disease which causes great economic losses worldwide. Many virulence factors are involved in the pathogenesis, namely capsular polysaccharides, RTX toxins, LPS and many iron acquisition systems. In order to identify genes that are expressed in vivo during a natural infection, we undertook transcript profiling experiments with an A. pleuropneumoniae DNA microarray, after recovery of bacterial mRNAs from serotype 5b-infected porcine lungs. AppChip2 contains 2033 PCR amplicons based on the genomic sequence of App serotype 5b strain L20, representing more than 95% of ORFs greater than 160 bp in length. Results Transcriptional profiling of A. pleuropneumoniae recovered from the lung of a pig suffering from a natural infection or following growth of the bacterial isolate in BHI medium was performed. An RNA extraction protocol combining beadbeating and hot-acid-phenol was developed in order to maximize bacterial mRNA yields and quality following total RNA extraction from lung lesions. Nearly all A. pleuropneumoniae transcripts could be detected on our microarrays, and 150 genes were deemed differentially expressed in vivo during the acute phase of the infection. Our results indicate that, for example, gene apxIVA from an operon coding for RTX toxin ApxIV is highly up-regulated in vivo, and that two genes from the operon coding for type IV fimbriae (APL_0878 and APL_0879 were also up-regulated. These transcriptional profiling data, combined with previous comparative genomic hybridizations performed by our group, revealed that 66 out of the 72 up-regulated genes are conserved amongst all serotypes and that 3 of them code for products that are predicted outer membrane proteins (genes irp and APL_0959, predicted to code for a TonB-dependent receptor and a filamentous hemagglutinin/adhesin respectively or lipoproteins (gene APL_0920. Only 4

  15. Improved diagnostic PCR assay for Actinobacillus pleuropneumoniae based on the nucleotide sequence of an outer membrane lipoprotein

    Gram, Trine; Ahrens, Peter

    1998-01-01

    species related to A. pleuropneumoniae or isolated from pigs were assayed. They were all found negative in the PCR, as were tonsil cultures from 50 pigs of an A. pleuropneumoniae-negative herd. The sensitivity assessed by agarose gel analysis of the PCR product was 10(2) CFU/PCR test tube. The specificity......The gene (omlA) coding for an outer membrane protein of Actinobacillus pleuropneumoniae serotypes 1 and 5 has been described earlier and has formed the basis for development of a specific PCR assay, The corresponding regions of all 12 A. pleuropneumoniae reference strains of biovar 1 were sequenced...... and sensitivity of this PCR compared to those of culture suggest the use of this PCR for routine identification of A. pleuropneumoniae....

  16. Transmission of Actinobacillus pleuropneumoniae in pigs under field-like conditions: emphasis on tonsillar colonisation and passively acquired colostral antibodies

    Vigre, Håkan; Angen, Øystein; Barfod, K.; Lavritsen, D. T.; Sørensen, V.

    proportion of pigs with detectable levels of colostral antibodies to the different serotypes of A. pleuropneumoniae was declining. Since these two events take place in the same age period, we expect a possible biological association between the level of the passive immunity and the degree of tonsillar......The objectives of this study were to elucidate at which age tonsillar colonisation by Actinobacillus pleuropneumoniae occurs in pigs and relate this occurrence to the presence of colostral antibodies to A. pleuropneumoniae. The infection patterns were studied in an isolated cohort of pigs, which...... birth, showing that A. pleuropneumoniae can be transmitted from sow to offspring during a 3-week nursing period. The cumulative proportion of pigs carrying A. pleuropneumoniae in their tonsils increased significantly between the age of 4-12 weeks. This age period corresponded to the age at which the...

  17. Effect of tulathromycin on the carrier status of Actinobacillus pleuropneumoniae serotype 2 in the tonsils of pigs

    Angen, Øystein; Andreasen, M.; Nielsen, E.O.;

    2008-01-01

    The effect of a single or double dose of tulathromycin was evaluated in pigs carrying Actinobacillus pleuropneumoniae serotype 2 in their tonsils. Twenty-nine pigs from a reinfected specific pathogen-free-herd were selected from animals testing positive in an A pleuropneumoniae serotype 2-specific...... were tested by PCR on tonsil scrapings on days 0, 4, 11 and 33, and on day 33 all the animals were euthanased. There were no significant differences between the numbers of PCR-positive animals in the three groups on any of the sampling dates....... PCR test on tonsil scrapings and they were divided into three groups. The pigs in group I were treated subcutaneously with 2.5 mg/kg tulathromycin on day 0, the pigs in group 2 were treated with 2.5 mg/kg tulathromycin on days 0 and 4, and the pigs in group 3 were left untreated as controls. The pigs...

  18. Comparative profiling of the transcriptional response to iron restriction in six serotypes of Actinobacillus pleuropneumoniae with different virulence potential

    Schou, Kirstine Klitgaard; Friis, Carsten; Angen, Øystein;

    2011-01-01

    receptor of Neisseria meningitidis, a possible virulence factor which contributes to bacterial survival in rats. Conclusions By comparative analysis of gene expression among 6 different serotypes of A. pleuropneumoniae we identified a common set of presumably essential core genes, involved in iron......Background Comparative analysis of gene expression among serotypes within a species can provide valuable information on important differences between related genomes. For the pig lung pathogen Actinobacillus pleuropneumoniae, 15 serotypes with a considerable variation in virulence potential and...... virulence genes. We used a pan-genomic microarray to study the transcriptional response to iron restriction in vitro in six serotypes of A. pleuropneumoniae (1, 2, 3, 5b, 6, and 7), representing at least two levels of virulence. Results In total, 45 genes were significantly (p <0.0001) up-regulated and 67...

  19. Microarray-based comparative genomic profiling of reference strains and selected Canadian field isolates of Actinobacillus pleuropneumoniae

    MacInnes Janet I

    2009-02-01

    Full Text Available Abstract Background Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, is a highly contagious respiratory pathogen that causes severe losses to the swine industry worldwide. Current commercially-available vaccines are of limited value because they do not induce cross-serovar immunity and do not prevent development of the carrier state. Microarray-based comparative genomic hybridizations (M-CGH were used to estimate whole genomic diversity of representative Actinobacillus pleuropneumoniae strains. Our goal was to identify conserved genes, especially those predicted to encode outer membrane proteins and lipoproteins because of their potential for the development of more effective vaccines. Results Using hierarchical clustering, our M-CGH results showed that the majority of the genes in the genome of the serovar 5 A. pleuropneumoniae L20 strain were conserved in the reference strains of all 15 serovars and in representative field isolates. Fifty-eight conserved genes predicted to encode for outer membrane proteins or lipoproteins were identified. As well, there were several clusters of diverged or absent genes including those associated with capsule biosynthesis, toxin production as well as genes typically associated with mobile elements. Conclusion Although A. pleuropneumoniae strains are essentially clonal, M-CGH analysis of the reference strains of the fifteen serovars and representative field isolates revealed several classes of genes that were divergent or absent. Not surprisingly, these included genes associated with capsule biosynthesis as the capsule is associated with sero-specificity. Several of the conserved genes were identified as candidates for vaccine development, and we conclude that M-CGH is a valuable tool for reverse vaccinology.

  20. Significance of CO2 donor on the production of succinic acid by Actinobacillus succinogenes ATCC 55618

    Zou Wei; Zhu Li-Wen; Li Hong-Mei; Tang Ya-Jie

    2011-01-01

    Abstract Background Succinic acid is a building-block chemical which could be used as the precursor of many industrial products. The dissolved CO2 concentration in the fermentation broth could strongly regulate the metabolic flux of carbon and the activity of phosphoenolpyruvate (PEP) carboxykinase, which are the important committed steps for the biosynthesis of succinic acid by Actinobacillus succinogenes. Previous reports showed that succinic acid production could be promoted by regulating ...

  1. Recombinant Actinobacillus actinomycetemcomitans Cytolethal Distending Toxin Proteins Are Required To Interact To Inhibit Human Cell Cycle Progression and To Stimulate Human Leukocyte Cytokine Synthesis

    Akifusa, Sumio; Poole, Stephen; Lewthwaite, Jo; Henderson, Brian; Nair, Sean P

    2001-01-01

    It has recently been discovered that Actinobacillus actinomycetemcomitans, an oral bacterium causing periodontitis, produces cytolethal distending toxin (CDT), a cell cycle-modulating toxin that has three protein subunits: CdtA, CdtB, and CdtC. In this study, we have cloned and expressed each toxin gene from A. actinomycetemcomitans in Escherichia coli and purified the recombinant Cdt proteins to homogeneity. Individual Cdt proteins failed to induce cell cycle arrest of the human epithelial c...

  2. Actinobacillus actinomycetemcomitans Y4 capsular-polysaccharide-like polysaccharide promotes osteoclast-like cell formation by interleukin-1 alpha production in mouse marrow cultures.

    Nishihara, T.; Ueda, N; Amano, K; Ishihara, Y; Hayakawa, H.; Kuroyanagi, T; Ohsaki, Y; Nagata, K.; Noguchi, T

    1995-01-01

    The mechanism of osteoclast-like cell formation induced by periodontopathic bacterium Actinobacillus actinomycetemcomitans Y4 (serotype b) capsular-polysaccharide-like polysaccharide (capsular-like polysaccharide) was examined in a mouse bone marrow culture system. When mouse bone marrow cells were cultured with A. actinomycetemcomitans Y4 capsular-like polysaccharide for 9 days, many multinucleated cells were formed. The multinucleated cells showed several characteristics of osteoclasts, inc...

  3. Mouse interleukin-1 receptor antagonist induced by Actinobacillus actinomycetemcomitans lipopolysaccharide blocks the effects of interleukin-1 on bone resorption and osteoclast-like cell formation.

    Nishihara, T; Ohsaki, Y.; Ueda, N; Saito, N; Mundy, G R

    1994-01-01

    We have reported that P388D1 cell line murine macrophages stimulated with lipopolysaccharide (LPS) from Actinobacillus actinomycetemcomitans release interleukin-1 (IL-1) inhibitor. The IL-1 inhibitor was purified from conditioned media of P388D1 cells stimulated with A. actinomycetemcomitans LPS for 72 h to homogeneity by a four-step procedure: acetic acid extraction from conditioned media; Bio-Gel P-60 gel filtration chromatography; DEAE-Sepharose CL-6B column chromatography; and reverse-pha...

  4. Antimicrobial effect of chlorine dioxide on Actinobacillus actinomycetemcomitans in diabetes mellitus rats treated with insulin

    Tantin Ermawati

    2012-03-01

    Full Text Available Background: Periodontitis is a chronic inflammatory disease of periodontal tissues. Etiology of periodontal disease includes Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans which is the most predominant disease-causing bacteria found in the gingival sulcus. Periodontitis can be exacerbated by the systemic disease, such as diabetes mellitus considered as a metabolic disease characterized by hyperglycemia due to insulin deficiency. Treatment of periodontitis is then required in patients with type I diabetes to avoid radical reaction that can not only cause bleeding, but can also prevent infection, as a result, topical antimicrobial therapy and blood glucose control are required. Topical antimicrobial chlorine dioxide is a disinfectant that is effective in killing A. actinomycetemcomitans. Purpose: This study is aimed to determine the effects of topical antimicrobial chlorine dioxide gel or rinse on the number of A. actinomycetemcomitans in DM rats treated with insulin. Methods: 20 three month old male Wistar rats with weight of 170–200 grams were divided into four groups. First, periodontitis and DM were manipulated into all groups through aloksan injection with dose of 170 mg/kg. Those rats in group I were treated with insulin and chlorine dioxide gel, those in group II were treated with insulin and chlorine dioxide rinse, those in group III were treated with insulin only, and those in group IV were without treatment. In the third and seventh weeks, the number of A. actinomycetemcomitans was measured. The data was tested by using One-Way ANOVA test followed by LSD test. Results: The study showed that chlorine dioxide gel has a greater ability in reducing the number of A. actinomycetemcomitans than chlorine dioxide rinse although both are antimicrobials. Conclusion: It can be concluded that the use of chlorine dioxide gel can more effective to decrease the number of A. actinomycetemcomitans than chlorine dioxide rinse in DM rats

  5. Performance analyses of a neutralizing agent combination strategy for the production of succinic acid by Actinobacillus succinogenes ATCC 55618.

    Wang, Cheng-Cheng; Zhu, Li-Wen; Li, Hong-Mei; Tang, Ya-Jie

    2012-05-01

    A neutralizing agent combination strategy was developed to enhance the succinic acid production by Actinobacillus succinogenes ATCC 55618. First, a maximal succinic acid production of 48.2 g/L was obtained at a culture pH of 7.5. Second, NaOH and KOH were screened to identify the optimal neutralizing agent for pH control. However, the production of succinic acid did not increase, and severe cell flocculation was observed due to a high concentration of metal ions when only one neutralizing agent was used to control pH. Finally, a neutralizing agent combination strategy was developed with a supply of neutralizing agents with OH(-) and carbonate. The cell flocculation was eliminated, and a maximum succinic acid production of 59.2 g/L was obtained with 5 M NaOH and 40 g/L of MgCO(3); this production was 27.9% higher than that obtained with NaOH alone. The results obtained in this study may be useful for the large-scale industrial production of succinic acid. PMID:22002101

  6. Actinobacillus succinogenes ATCC 55618 fermentation medium optimization for the production of succinic acid by response surface methodology.

    Zhu, Li-Wen; Wang, Cheng-Cheng; Liu, Rui-Sang; Li, Hong-Mei; Wan, Duan-Ji; Tang, Ya-Jie

    2012-01-01

    As a potential intermediary feedstock, succinic acid takes an important place in bulk chemical productions. For the first time, a method combining Plackett-Burman design (PBD), steepest ascent method (SA), and Box-Behnken design (BBD) was developed to optimize Actinobacillus succinogenes ATCC 55618 fermentation medium. First, glucose, yeast extract, and MgCO(3) were identified to be key medium components by PBD. Second, preliminary optimization was run by SA method to access the optimal region of the key medium components. Finally, the responses, that is, the production of succinic acid, were optimized simultaneously by using BBD, and the optimal concentration was located to be 84.6 g L(-1) of glucose, 14.5 g L(-1) of yeast extract, and 64.7 g L(-1) of MgCO(3). Verification experiment indicated that the maximal succinic acid production of 52.7 ± 0.8 g L(-1) was obtained under the identified optimal conditions. The result agreed with the predicted value well. Compared with that of the basic medium, the production of succinic acid and yield of succinic acid against glucose were enhanced by 67.3% and 111.1%, respectively. The results obtained in this study may be useful for the industrial commercial production of succinic acid. PMID:23093852

  7. Impact of Actinobacillus pleuropneumoniae biofilm mode of growth on the lipid A structures and stimulation of immune cells.

    Hathroubi, Skander; Beaudry, Francis; Provost, Chantale; Martelet, Léa; Segura, Mariela; Gagnon, Carl A; Jacques, Mario

    2016-07-01

    Actinobacillus pleuropneumoniae (APP), the etiologic agent of porcine pleuropneumonia, forms biofilms on biotic and abiotic surfaces. APP biofilms confers resistance to antibiotics. To our knowledge, no studies have examined the role of APP biofilm in immune evasion and infection persistence. This study was undertaken to (i) investigate biofilm-associated LPS modifications occurring during the switch to biofilm mode of growth; and (ii) characterize pro-inflammatory cytokines expression in porcine pulmonary alveolar macrophages (PAMs) and proliferation in porcine PBMCs challenged with planktonic or biofilm APP cells. Extracted lipid A samples from biofilm and planktonic cultures were analyzed by HPLC high-resolution, accurate mass spectrometry. Biofilm cells displayed significant changes in lipid A profiles when compared with their planktonic counterparts. Furthermore, in vitro experiments were conducted to examine the inflammatory response of PAMs exposed to UV-inactivated APP grown in biofilm or in suspension. Relative mRNA expression of pro-inflammatory genes IL1, IL6, IL8 and MCP1 decreased in PAMs when exposed to biofilm cells compared to planktonic cells. Additionally, the biofilm state reduced PBMCs proliferation. Taken together, APP biofilm cells show a weaker ability to stimulate innate immune cells, which could be due, in part, to lipid A structure modifications. PMID:27226465

  8. Construction and immunogenicity of a ∆apxIC/ompP2 mutant of Actinobacillus pleuropneumoniae and Haemophilus parasuis

    Qiong Liu

    2013-03-01

    Full Text Available The apxIC genes of the Actinobacillus pleuropneumoniae serovar 5 (SC-1, encoding the ApxIactivating proteins, was deleted by a method involving sucrose counter-selection. In this study, a mutant strain of A. pleuropneumoniae (SC-1 was constructed and named DapxIC/ ompP2. The mutant strain contained foreign DNA in the deletion site of ompP2 gene of Haemophilus parasuis. It showed no haemolytic activity and lower virulence of cytotoxicity in mice compared with the parent strain, and its safety and immunogenicity were also evaluated in mice. The LD50 data shown that the mutant strain was attenuated 30-fold, compared with the parent strain (LD50 of the mutant strain and parent strain in mice were determined to be 1.0 × 107 CFU and 3.5 × 105 CFU respectively. The mutant strain that was attenuated could secrete inactivated ApxIA RTX toxins with complete antigenicity and could be used as a candidate live vaccine strain against infections of A. pleuropneumoniae and H. parasuis.

  9. Identification of a hemolysin from Actinobacillus pleuropneumoniae and characterization of its channel properties in planar phospholipid bilayers.

    Lalonde, G; McDonald, T V; Gardner, P; O'Hanley, P D

    1989-08-15

    A proteinaceous hemolysin secreted by strain 4074 of serotype 1 of Actinobacillus pleuropneumoniae was purified by diafiltration and ion exchange chromatographic techniques. The hemolytic activity is associated with a 107-kDa band as assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and confirmed by Western blotting and immunoprecipitation. This hemolysin produces pores in membranes as demonstrated by osmotic protection studies using red blood cells and carbohydrate compounds of various molecular weights. These assays suggest a pore diameter in the order of 2 nm. Phospholipid bilayers composed of 1:1 w/w phosphotidylserine:phosphotidylethanolamine exposed to this toxin display discrete current flow events typical of transmembrane channels and consistent with the interpretation that this toxin acts by forming pores in phospholipid membranes. The linear relationship of current amplitude to holding potential when examined over the -60 to +60 mV range indicates that this pore has a constant mean single channel conductance level of 350-400 pS. PMID:2474533

  10. Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: a new insight into an old approach

    Ciro César Rossi

    2013-01-01

    Full Text Available The OmlA protein is a virulence factor of Actinobacillus pleuropneumoniae, an important pathogen in pigs. The polymorphisms present in the omlA gene sequence of 15 reference serotypes of A. pleuropneumoniae and non-serotypable isolates were assessed to determine the possible evolutionary relationship among them and to validate the importance of this gene as a molecular marker for the characterization of this bacterium. Divergence among the 15 serotypes of A. pleuropneumoniae probably resulted initially from two major evolutionary events that led to subsequent differentiation into nine groups. This differentiation makes it possible to characterize most of the serotypes by using bionformatics, thereby avoiding problems with immunological cross-reactivity. A conserved α-helix common to all the serotypes was most likely involved in connecting the protein to the outer membrane and acting as a signal peptide. A previously unknown gene duplication was also identified and could contribute to the genetic variability that makes it difficult to serotype some isolates. Our data support the importance of the omlA gene in the biology of A. pleuropneumoniae and provide a new area of research into the OmlA protein.

  11. Bagasse hydrolyzates from Agave tequilana as substrates for succinic acid production by Actinobacillus succinogenes in batch and repeated batch reactor.

    Corona-González, Rosa Isela; Varela-Almanza, Karla María; Arriola-Guevara, Enrique; Martínez-Gómez, Álvaro de Jesús; Pelayo-Ortiz, Carlos; Toriz, Guillermo

    2016-04-01

    The aim of this work was to obtain fermentable sugars by enzymatic or acid hydrolyses of Agave tequilana Weber bagasse in order to produce succinic acid with Actinobacillus succinogenes. Hydrolyses were carried out with mineral acids (sulfuric and hydrochloric acids) or a commercial cellulolytic enzyme, and were optimized statistically by a response surface methodology, having as factors the concentration of acid/enzyme and time of hydrolysis. The concentration of sugars obtained at optimal conditions for each hydrolysis were 21.7, 22.4y 19.8g/L for H2SO4, HCl and the enzymatic preparation respectively. Concerning succinic acid production, the enzymatic hydrolyzates resulted in the highest yield (0.446g/g) and productivity (0.57g/Lh) using A. succinogenes in a batch reactor system. Repeated batch fermentation with immobilized A. succinogenes in agar and with the enzymatic hydrolyzates resulted in a maximum concentration of succinic acid of 33.6g/L from 87.2g/L monosaccharides after 5 cycles in 40h, obtaining a productivity of 1.32g/Lh. PMID:26802183

  12. Use of corn steep liquor as an economical nitrogen source for biosuccinic acid production by Actinobacillus succinogenes

    Tan, J. P.; Jahim, J. M.; Wu, T. Y.; Harun, S.; Mumtaz, T.

    2016-06-01

    Expensive raw materials are the driving force that leads to the shifting of the petroleum-based succinic acid production into bio-based succinic acid production by microorganisms. Cost of fermentation medium is among the main factors contributing to the total production cost of bio-succinic acid. After carbon source, nitrogen source is the second largest component of the fermentation medium, the cost of which has been overlooked for the past years. The current study aimed at replacing yeast extract- a costly nitrogen source with corn steep liquor for economical production of bio-succinic acid by Actinobacillus succinogenes 130Z. In this study, a final succinic acid concentration of 20.6 g/L was obtained from the use of corn steep liquor as the nitrogen source, which was comparable with the use of yeast extract as the nitrogen source that had a final succinate concentration of 21.4 g/l. In terms of economical wise, corn steep liquor was priced at 200 /ton, which was one fifth of the cost of yeast extract at 1000 /ton. Therefore, corn steep liquor can be considered as a potential nitrogen source in biochemical industries instead of the costly yeast extract.

  13. Activity of antibodies against Salmonella dublin, Toxoplasma gondii, or Actinobacillus pleuropneumoniae in sera after treatment with electron beam irradiation or binary ethylenimine

    Kyvsgaard, N.C.; Lind, Peter; Preuss, T.; Kamstrup, Søren; Lei, J.C.; Bogh, H.O.; Nansen, P.

    1996-01-01

    used as an estimate for the relative posttreatment activity. For a Toxoplasma gondii indirect enzyme-linked immunosorbent assay (ELISA) and agglutination assay as well as for a Salmonella dublin indirect ELISA, the posttreatment activity was more than 89% of the pretreatment activity when the samples...... were irradiated in the frozen state on dry ice but only 35% of their activity when they were irradiated in the liquid state at 0 degrees C. The patterns seen in an S. dublin blocking ELISA and an Actinobacillus pleuropneumoniae complement fixation assay differed in that samples with a low level of...

  14. No overall relationship between average daily weight gain and the serological response to Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae in eight chronically infected Danish swine herds

    Andreasen, Margit; Mousing, Jan; Thomsen, Lars Krogsgård

    approximately 4 weeks of age), and sera were analyzed for antibodies to Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae serotypes 2, 5-7 and 12. Mixed analysis of covariance analyzed the relationship between the average daily weight gain and a categorical variable defining seroconversion as none...... most pigs included in the study were subclinically infected, or because a temporary negative influence of the infections is hidden due to an increased growth in the period following infection. In conclusion. at least in these eight herds, seroresponses to M. hyopneumoniae and A. pleuropneumoniae could...

  15. LEUKOTOXIC ACTIVITY OF ACTINOBACILLUS ACTINOMYCETEMCOMITANS ISOLATED FROM HUMAN AND NON-HUMAN PRIMATES Atividade leucotóxica de amostras de Actinobacillus actinomycetemcomitans de primatas humanos não-humanos

    Francisca Lúcia de Lima

    2001-10-01

    Full Text Available Actinobacillus actinomycetemcomitans is a clinically relevant periodontopathogenic Gram-negative coccobacillus that produces a leukotoxin of the RTX cytolysin family. In this study, we evaluated the leukotoxic activity of A. actinomycetemcomitans strains isolated from human and marmosets by Trypan blue exclusion and by the chemiluminescence assays. Among eight A. actinomycetemcomitans human strains studied, two (P2.17 and P8.12 were classified as high leukotoxin producers and among eight marmoset strains, one (M22.11 showed high leukotoxin production, as determined by Trypan blue exclusion assay. The reference strains ATCC 29523 and FDC Y4 respectively behaved like moderate and low producers. The chemiluminescence assay was used to evaluate the leukotoxic activity of M22.11 and P2.17 strains submitted to different growth conditions. Leukotoxic activity was detected on cells at the logarithmic phase and was similar under anaerobic and microaerophilic growth conditions. It was greatly reduced when cells were grown at glucose concentrations lower or higher than 0.75% (0.25% and 1.5% in thioglycolate medium. Leukotoxin production mainly by the M22.11 strain was low in BHI broth, whereas production in TSB medium showed a similar level as in thioglycolate broth medium. Sodium bicarbonate at 10 mM did not affect leukotoxin production.Actinobacillus actinomycetemcomitans é um cocobacilo Gram negativo, periodontopatógeno clinicamente importante, que produz uma leucotoxina pertencente à família das citolisinas RTX. Neste estudo, avaliou-se a atividade leucotóxica de amostras de A. actinomycetemcomitans isoladas de seres humanos e de calitriquídeos pelos métodos de exclusão de azul de Tripan e quimioluminescência. Duas (P2.17 e P8.12 entre oito amostras de A. actinomycetemcomitans isoladas de seres humanos, e uma (M22.11 entre 8 amostras isoladas de sagüis se apresentaram como altamente produtoras de leucotoxina, como determinado pelo teste de

  16. Diversidad genética de cepas de Actinobacillus pleuropneumoniae (App aisladas desde planteles de producción intensiva de cerdos en Chile Genetic diversity of Actinobacillus pleuropneumoniae (App strains in intensive swine farms in Chile

    V Neira-Ramírez

    2012-01-01

    Full Text Available Actinobacillus pleuropneumoniae (App es el agente etiológico de la pleuroneumonía contagiosa porcina, una de las enfermedades de etiología bacteriana de mayor relevancia en producción porcina. En el mundo se han descrito 15 serotipos de App, en Chile solo los serotipos 1 y 5. La serotipificación requiere mucho tiempo, trabajo y dinero, actualmente se encuentran herramientas moleculares para realizar una "serotipificación" mediante la genotipificación de toxinas Apx. Así, se evaluaron 60 aislados de App provenientes de nueve empresas porcinas de producción intensiva distribuidas en distintas regiones de Chile, obtenidas desde pulmones de cerdos con lesiones compatibles con pleuroneumonía contagiosa porcina. Las bacterias fueron aisladas mediante los métodos tradicionales y confirmados por API, recolectados durante los años 2007, 2008 y 2009. Los resultados identificaron los genotipos correspondientes sólo a los serotipos 4, 6 y 7, los cuales se describen por primera vez en Chile, siendo el más frecuente el serotipo 7. En las diferentes zonas estudiadas, no existió un serotipo predominante, excepto en las regiones de O'Higgins y del Biobío en las cuales fue más frecuentemente aislado el serotipo 7. El presente estudio es el primer acercamiento con el fin de conocer la distribución de serotipos de App en Chile. Con el fin de conocer la real diversidad genética y serotipos de App en los diversos planteles en Chile es necesario realizar estudios que contemplen un mayor número de aislados.Actinobacillus pleuropneumoniae (App is the etiologic agent of porcine contagious pleuropneumonia, an important bacterial disease in intensive pig production. In the world were described 15 App serotypes, in Chile serotypes 1 and 5 have been reported. The serotyping technique is slow, expensive and difficult; currently, a molecular tool named PCR is available to "serotyping" by Apx toxins genotyping, which is quick, non-expensive and easy. 60 App

  17. The porcine acute phase response to infection with Actinobacillus pleuropneumoniae. Haptoglobin, C-reactive protein, major acute phase protein and serum amyloid a protein are sensitive indicators of infection

    Heegaard, Peter M. H.; Klausen, Joan; Nielsen, J.P.;

    1998-01-01

    In an experimental infection model mimicking acute Actinobacillus pleuropneumoniae (Ap) infection in swine (Sus scrofa) by aerosol inoculation, the development of a number of typical clinical signs was accompanied by a prototypic acute phase reaction encompassing fever and an acute phase protein ...

  18. Association between coinfection of Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Treponema denticola and periodontal tissue destruction in chronic periodontitis

    CHEN Li-li; WU Yan-min; YAN Jie; SUN Wei-lian; SUN Yu-zheng; David Ojcius

    2005-01-01

    Background The association between the infection of Porphyromonas gingivalis, Actinobacillus actinomy-cetemcomitans and Treponema denticola in chronic periodontitis (CP) and the severity of periodontal disease remains to be elucidated. The aim of this study was to investigate the subgingival infection frequencies of three periodontopathic bacteria in Chinese CP patients and to evaluate the correlations between infection by these bacteria and periodontal destruction.Methods A multiple PCR assay using primers derived from 16SrDNA genes of P. gingivalis, A. actinomy-cetemitans and T. denticola was established to measure simultaneously the presence of the three microbes in 162 subgingival samples from 81 Chinese CP patients. Results The positive rates of P. gingivalis, A. actinomycetemitans and T. denticola in the subgingival samples were 84.6%, 83.3% and 88.3%, respectively. Of the subgingival samples, 68% revealed the coinfection of all the three microbes. The infection rates with P. gingivalis, A. actinomycetemitans or T. denticola alone was 5.9% (1/17), 17.6% (3/17) and 76.5% (13/17), respectively. A close association was present between the A. actinomycetemitans infection and gingival index (GI) (P0.05). P. gingivalis and A. actinomycetemitans were more frequently detectable in middle and deep pockets than in shallow ones (P<0.01), while T. denticola was found remarkably often in deep pockets (P<0.05). The coinfection rate of the three microbes was significantly higher in sites with severe periodontitis than in those with mild periodontitis (P<0.01). Conclusions The multiple PCR established in this study can be used as a sensitive and specific method to simultaneously detect all three microbes in subgingival samples. A. actinomycetemitans infection may be associated with CP and play an important role in the periodontal tissue destruction. The coinfection of P. gingivalis, A. actinomycetemitans and T. denticola can cause more serious periodontal destruction than

  19. Comparative profiling of the transcriptional response to iron restriction in six serotypes of Actinobacillus pleuropneumoniae with different virulence potential

    Angen Øystein

    2010-12-01

    Full Text Available Abstract Background Comparative analysis of gene expression among serotypes within a species can provide valuable information on important differences between related genomes. For the pig lung pathogen Actinobacillus pleuropneumoniae, 15 serotypes with a considerable variation in virulence potential and immunogenicity have been identified. This serotypic diversity can only partly be explained by amount of capsule and differences in the RTX toxin genes in their genomes. Iron acquisition in vivo is an important bacterial function and in pathogenic bacteria, iron-limitation is often a signal for the induction of virulence genes. We used a pan-genomic microarray to study the transcriptional response to iron restriction in vitro in six serotypes of A. pleuropneumoniae (1, 2, 3, 5b, 6, and 7, representing at least two levels of virulence. Results In total, 45 genes were significantly (p A. pleuropneumoniae was the up-regulation of a putative cirA-like siderophore in all six serotypes. Three genes, recently described in A. pleuropneumoniae as possibly coding for haemoglobin-haptoglobin binding proteins, displayed significant serotype related up-regulation to iron limitation. For all three genes, the expression appeared at its lowest in serotype 3, which is generally considered one of the least virulent serotypes of A. pleuropneumoniae. The three genes share homology with the hmbR haemoglobin receptor of Neisseria meningitidis, a possible virulence factor which contributes to bacterial survival in rats. Conclusions By comparative analysis of gene expression among 6 different serotypes of A. pleuropneumoniae we identified a common set of presumably essential core genes, involved in iron regulation. The results support and expand previous observations concerning the identification of new potential iron acquisition systems in A. pleuropneumoniae, showing that this bacterium has evolved several strategies for scavenging the limited iron resources of the

  20. Molecular characterisation of the early response in pigs to experimental infection with Actinobacillus pleuropneumoniae using cDNA microarrays

    Bendixen Christian

    2007-04-01

    Full Text Available Abstract Background The bacterium Actinobacillus pleuropneumoniae is responsible for porcine pleuropneumonia, a widespread, highly contagious and often fatal respiratory disease of pigs. The general porcine innate immune response after A. pleuropneumoniae infection is still not clarified. The objective of this study was hence to characterise the transcriptional response, measured by using cDNA microarrays, in pigs 24 hours after experimental inoculation with A. pleuropneumoniae. Methods Microarray analyses were conducted to reveal genes being differentially expressed in inflamed versus non-inflamed lung tissue sampled from inoculated animals as well as in liver and tracheobronchial lymph node tissue sampled from three inoculated animals versus two non-inoculated animals. The lung samples were studied using a porcine cDNA microarray with 5375 unique PCR products while liver tissue and tracheobronchial lymph node tissue were hybridised to an expanded version of the porcine microarray with 26879 unique PCR products. Results A total of 357 genes differed significantly in expression between infected and non-infected lung tissue, 713 genes differed in expression in liver tissue from infected versus non-infected animals and 130 genes differed in expression in tracheobronchial lymph node tissue from infected versus non-infected animals. Among these genes, several have previously been described to be part of a general host response to infections encoding immune response related proteins. In inflamed lung tissue, genes encoding immune activating proteins and other pro-inflammatory mediators of the innate immune response were found to be up-regulated. Genes encoding different acute phase reactants were found to be differentially expressed in the liver. Conclusion The obtained results are largely in accordance with previous studies of the mammalian immune response. Furthermore, a number of differentially expressed genes have not previously been associated

  1. Conservation and antigenic cross-reactivity of the transferrin-binding proteins of Haemophilus influenzae, Actinobacillus pleuropneumoniae and Neisseria meningitidis.

    Holland, J; Parsons, T R; Hasan, A A; Cook, S M; Stevenson, P; Griffiths, E; Williams, P

    1996-12-01

    Haemophilus influenzae acquires iron from the iron-transporting glycoprotein transferrin via a receptor-mediated process. This involves two outer-membrane transferrin-binding proteins (Tbps) termed Tbp1 and Tbp2 which show considerable preference for the human form of transferrin. Since the Tbps are attracting considerable attention as potential vaccine components, we used transferrin affinity chromatography to examine their conservation amongst 28 H. influenzae type b strains belonging to different outer-membrane-protein subtypes as well as six non-typable strains. Whole cells of all type b and non-typable strains examined bound human transferrin; whilst most strains possessed a Tbp1 of approximately 105 kDa, the molecular mass of Tbp2 varied from 79 to 94 kDa. Antisera raised against affinity-purified native H. influenzae Tbp1/Tbp2 receptor complex cross-reacted on Western blots with the respective Tbps of all the Haemophilus strains examined. When used to probe Neisseria meningitidis Tbps, sera from each of four mice immunized with the Haemophilus Tbp1/2 complex recognized the 68 kDa Tbp2 of N. meningitidis strain B16B6 but not the 78 kDa Tbp2 of N. meningitidis strain 70942. Serum from one mouse also reacted weakly with Tbp1 of strain B16B6. Apart from a weak reaction with the Tbp2 of a serotype 5 strain, this mouse antiserum failed to recognize the Tbps of the porcine pathogen A. pleuropneumoniae. However, a monospecific polyclonal antiserum raised against the denatured Tbp2 of Neisseria meningitidis B16B6 recognized the Tbps of all Haemophilus and Actinobacillus strains examined. Since H. influenzae forms part of the natural flora of the upper respiratory tract, human sera were screened for the presence of antibodies to the Tbps. Sera from healthy adults contained antibodies which recognized both Tbp1 and Tbp2 from H. influenzae but not N. meningitidis. Convalescent sera from meningococcal meningitis patients contained antibodies which, on Western blots

  2. Economic impacts of reduced pork production associated with the diagnosis of Actinobacillus pleuropneumoniae on grower/finisher swine operations in the United States.

    Losinger, Willard C

    2005-05-10

    An examination of the economic impacts of the diagnosis of Actinobacillus pleuropneumoniae on grower/finisher swine operations indicated that reduced pork production, associated with the diagnosis of A. pleuropneumoniae on the operation, diminished consumer surplus by $53+/-52 million, and resulted in a total loss of $32+/-30 million to the US economy in 1995. Most of the economic surplus lost by consumers was transferred to producers, whose economic surplus increased by $21+/-25 million (which was not significantly different from zero). Uncertainty analysis showed that an estimate of the decline in production associated with the diagnosis of A. pleuropneumoniae accounted for most of the uncertainty of the change in consumer surplus and of the total loss to the economy. The estimate of the price elasticity of demand for pork also contributed towards a lot of the uncertainty in the estimated change in producer surplus. PMID:15820115

  3. Intra-unit correlations in seroconversion to Actinobacillus pleuropneumoniae and Mycoplasma hyopneumoniae at different levels in Danish multi-site pig production facilities

    Vigre, Håkan; Dohoo, I.R.; Stryhn, H.;

    2004-01-01

    2) and Mycoplasma hyopneumoniae (Mh). Based on the estimated variances, three newly described computational methods (model linearisation, simulation and linear modelling) and the standard method (latent-variable approach) were used to estimate the correlations (intra-class correlation components......, ICCs) between pigs in the same production unit regarding seroconversion. Substantially different values of ICCs were obtained from the four methods. However, ICCs obtained by the simulation and the model linearisation were quite consistent. Data used for estimation were collected from 1161 pigs from......In this paper, multilevel logistic models which take into account the multilevel structure of multi-site pig production were used to estimate the variances between pigs produced in Danish multi-site pig production facilities regarding seroconversion to Actinobacillus pleuropneumoniae serotype 2 (Ap...

  4. Expresión recombinante en E. Coli de antígenos de Actinobacillus Pleuropneumoniae para vacunación y diagnóstico

    Medrano Muñoz, Andrés

    2003-01-01

    Actinobacillus pleuropneumoniae es una bacteria gramnegativa que provoca la pleuroneumonía porcina. En este trabajo se ha procedido a la producción y purificación, mediante técnicas de biología molecular, de antígenos proteicos de esta bacteria y a su uso en la formulación de una vacuna por subunidades y de un ELISA para diagnóstico. Los cuatro antígenos escogidos fueron dos proteínas de membrana externa (Tbp1 y Tbp2) y dos exotoxinas (ApxI y ApxIII). La primera necesidad consistía en localiz...

  5. Incidence of Reinfections with Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae in Pig Farms Located in Respiratory-Disease-Free Regions of Switzerland – Identification and Quantification of Risk Factors

    Scheidegger R

    2002-09-01

    Full Text Available The objective of the study was to identify risk factors for reintroduction of Actinobacillus pleuopneumoniae and Mycoplasma hyopneumoniae (enzootic pneumonia onto pig farms in areas in Switzerland that were involved in an eradication programme from 1996 to 1999 and to assess the role of dealers in relation to these reinfections. The study was based on the comparison of pig farms that were reinfected in the year 2000 (cases and pig farms that remained uninfected in the same area (controls. Additionally, data were collected from Swiss pig dealers and transport companies. Out of a total of 3983 farms, 107 farms were reinfected in the year 2000. The incidences were 0.1% for Actinobacillus pleuopneumoniae and 2.6% for Mycoplasma hyopneumoniae (enzootic pneumonia. Compared to reinfection rates prior to the eradication programme, this is a considerable reduction. Statistically significant risk factors for the reinfection were 'finishing farm', 'large mixed breeding-finishing farm', 'reinfected neighbour' and 'parking site for pig transport vehicles close to the farm'. Pig farmers that purchased pigs from only one supplier per batch had a lower risk of reintroducing infection (protective factor. As long as infected and uninfected regions co-exist in Switzerland, direct and indirect contact between farms, pig herds and slaughter sites via transport vehicles are a major pathway of disease spread. Risk management measures linked to these contacts are therefore of key importance. The survey of dealers indicated various areas for improvement such as strategic planning of pick-up routes or cleaning and disinfecting of trucks.

  6. Estudio del comportamiento serológico de Actinobacillus pleuropneumoniae (App en planteles porcinos comerciales de la zona central de Chile Serological behaviour study of Actinobacillus pleuropneumoniae (App in commercial swine herds from the central region of Chile

    D Muñoz

    2008-01-01

    Full Text Available En Chile se ha realizado sólo un estudio en Actinobacillus pleuropneumoniae (App. Este trabajo pretende determinar la duración de la inmunidad materna, la edad de seroconversión y la prevalencia aparente y verdadera en 7 planteles de cerdos comerciales. Se obtuvieron 60 muestras por plantel, divididas en 10 muestras de suero, de animales de 4, 6, 10, 14,18 y 21 semanas de edad, y analizadas a través de un kit ELISA® comercial. De las 420 muestras se detectaron 134 positivas, de las cuales 112 correspondían a cerdos menores de 10 semanas y sólo 22 provenían de animales mayores de 10 semanas, que seroconvirtieron probablemente debido a una infección de campo. La caída de la inmunidad materna fue alrededor de la 10ª semana de edad. En cuanto a la seroconversión, se observó que a partir de la 18* semana comenzaron a aparecer los animales con anticuerpos circulantes propios. Dos de los siete planteles no seroconvirtieron. Además, dos presentaron una seroconversión igual o superior al 50% a las 18 semanas. La seroprevalencia aparente de App fue de 10,48%, mientras que prevalencia verdadera, mediante dos métodos estadísticos, fue de 9,6% (IC: 7,6% y 11,7% y 10,67% respectivamente. En este trabajo se encontró que la prevalencia es similar a la observada en EE.UU., debido presumiblemente al sistema de producción y a los serotipos que están presentes en ambos países. Por otro lado, si bien la mayoría de los planteles seroconvierten luego de la caída de la inmunidad materna, se observaron diferentes patrones serológicos entre ellos.In Chile, there was only one existing study on App. This study was designed to determine the maternal immunity duration, the age of seroconversion and the apparent and true prevalence in animals from 7 swine commercial herds. 60 samples were taken per herd and divided into 10 serum samples from animals of 4, 6,10,14,18and21 weeks of age, which were analyzed by ELISA®. Out of the 420 samples, 134 were

  7. Occurrence of Actinobacillus actinomycetemcomitans in patients with chronic periodontitis, aggressive periodontitis, healthy subjects and children with gingivitis in two cities of the state of São Paulo, Brazil Ocorrência de Actinobacillus actinomycetemcomitans em pacientes com periodontite crônica, periodontite agressiva, pessoas saudáveis e crianças com gengivite em duas cidades do Estado de São Paulo, Brasil

    Elerson Gaetti Jardim Júnior; Joseane Maria Dias Bosco; Angélica Marquezim Lopes; Luís Fernando Landucci; Ellen Cristina Gaetti Jardim; Sílvia Rosana Soares Carneiro

    2006-01-01

    The aim of this study was to determine the frequency of isolation of Actinobacillus actinomycetemcomitans (Aa) in 100 patients with chronic periodontitis, 14 patients with aggressive periodontitis, 142 pre-school children with gingivitis and 134 periodontally healthy subjects. Samples of subgingival plaque were taken using sterilized paper points introduced into periodontal pockets or gingival crevice for 60 seconds and inoculated on TSBV agar, which was incubated under anaerobiosis at 37ºC, ...

  8. The Actinobacillus pleuropneumoniae HMW1C-like glycosyltransferase mediates N-linked glycosylation of the Haemophilus influenzae HMW1 adhesin.

    Kyoung-Jae Choi

    Full Text Available The Haemophilus influenzae HMW1 adhesin is an important virulence exoprotein that is secreted via the two-partner secretion pathway and is glycosylated at multiple asparagine residues in consensus N-linked sequons. Unlike the heavily branched glycans found in eukaryotic N-linked glycoproteins, the modifying glycan structures in HMW1 are mono-hexoses or di-hexoses. Recent work demonstrated that the H. influenzae HMW1C protein is the glycosyltransferase responsible for transferring glucose and galactose to the acceptor sites of HMW1. An Actinobacillus pleuropneumoniae protein designated ApHMW1C shares high-level homology with HMW1C and has been assigned to the GT41 family, which otherwise contains only O-glycosyltransferases. In this study, we demonstrated that ApHMW1C has N-glycosyltransferase activity and is able to transfer glucose and galactose to known asparagine sites in HMW1. In addition, we found that ApHMW1C is able to complement a deficiency of HMW1C and mediate HMW1 glycosylation and adhesive activity in whole bacteria. Initial structure-function studies suggested that ApHMW1C consists of two domains, including a 15-kDa N-terminal domain and a 55-kDa C-terminal domain harboring glycosyltransferase activity. These findings suggest a new subfamily of HMW1C-like glycosyltransferases distinct from other GT41 family O-glycosyltransferases.

  9. Identification and Detection of Actinobacillus pleuropneumoniae in Infected and Subclinically Infected Pigs by Multiplex PCR Based on the Genes ApxIVA and OmlA

    XIAO Guo-sheng; CAO San-jie; DUAN Li-li; WEN Xin-tian; MA Xiao-ping; CHEN Hua-mei

    2006-01-01

    PCRs based on different genes of Actinobacillus pleuropneumoniae have been developed for detecting and identifying A. pleuropneumoniae. Some of them could amplify positive fragments from the phylogenetically closely related species bacteria. To improve veracity and specificity of PCR, a species-specific multiplex PCR assay was developed to identify and detect A. pleuropneumoniae, based on the 3'-terminus of the species-specific apxIVA gene and the already existing species-specific primers in the omlA gene. Both 346-bp and 950-bp fragments could be simultaneously amplified from all A. pleuropneumoniae reference strains and isolates, and the species specificity of the assay was evaluated with a collection of ten strains representing eight different species bacteria including species normally found in the respiratory tracts of swine. All of these strains turned out negative in the multiplex PCR. All sequences of products of multiplex PCR randomly sampled were also correct. The sensitivity of the multiplex PCR was determined to be 10 pg ofA. pleuropneumoniae DNA. The multiplex PCR and bacterial isolation were compared to determine their sensitivities by using experimentally infected pigs and clinical disease pigs. The multiplex PCR was more sensitive than bacterial isolation. The multiplex PCR was also evaluated on mixed bacterial cultures from clinical healthy pigs. 26/100 (26%) of the subclinically infected pigs were detected from clinical healthy pigs. The results indicate that the multiplex PCR assay is a sensitive, highly specific,and effective diagnostic tool for identification and detection of A. pleuropneumoniae.

  10. Effect of different antimicrobial treatments on serum acute phase responses and leukocyte counts in pigs after a primary and a secondary challenge infection with Actinobacillus pleuropneumoniae

    Sjölund, M; Fossum, C; Martin de la Fuente, AJM;

    2011-01-01

    The susceptibility to an initial challenge and a re-challenge inoculation with Actinobacillus pleuropneumoniae was analysed in pigs that were treated with antimicrobials of different efficacies following the first exposure to A pleuropneumoniae. In brief, 30 nine-week-old specific pathogen......-free pigs were allocated to five groups of six. After acclimatisation, four groups were inoculated with A pleuropneumoniae serotype 2. At the onset of clinical signs, three of the groups of pigs were treated with enrofloxacin, tetracycline or penicillin. A fourth group served as the inoculated control and...

  11. Differences in Purinergic Amplification of Osmotic Cell Lysis by the Pore-Forming RTX Toxins Bordetella pertussis CyaA and Actinobacillus pleuropneumoniae ApxIA: the Role of Pore Size

    Mašín, Jiří; Fišer, Radovan; Linhartová, Irena; Osička, Radim; Bumba, Ladislav; Hewlett, E. L.; Benz, R.; Šebo, Peter

    2013-01-01

    Roč. 81, č. 12 (2013), s. 4571-4582. ISSN 0019-9567 R&D Projects: GA ČR GAP302/12/0460; GA ČR(CZ) GAP302/11/0580; GA ČR(CZ) GAP207/11/0717; GA AV ČR IAA500200914; GA ČR GA13-14547S Institutional support: RVO:61388971 Keywords : Bordetella pertussis * Actinobacillus pleuropneumoniae * E-coli Subject RIV: EE - Microbiology, Virology Impact factor: 4.156, year: 2013

  12. Usefulness of real time PCR for the differentiation and quantification of 652 and JP2 Actinobacillus actinomycetemcomitans genotypes in dental plaque and saliva

    Piras Vincenzo

    2006-06-01

    Full Text Available Abstract Background The aim of our study is to describe a fast molecular method, able to distinguish and quantize the two different genotypes (652 and JP2 of an important periodontal pathogen: Actinobacillus actinomycetemcomitans. The two genotypes show differences in the expression of an important pathogenic factor: the leukotoxin (ltx. In order to evidence this, we performed a real time PCR procedure on the ltx operon, able to recognize Aa clinical isolates with different leukotoxic potentials. Methods The specificity of the method was confirmed in subgingival plaque and saliva specimens collected from eighty-one Italian (Sardinian subjects with a mean age of 43.9, fifty five (68 % of whom had various clinical forms of periodontal disease. Results This procedure showed a good sensitivity and a high linear dynamic range of quantization (107-102 cells/ml for all genotypes and a good correlation factor (R2 = 0.97–0.98. Compared with traditional cultural methods, this real time PCR procedure is more sensitive; in fact in two subgingival plaque and two positive saliva specimens Aa was only detected with the molecular method. Conclusion A low number of Sardinian patients was found positive for Aa infections in the oral cavity, (just 10 positive periodontal cases out of 81 and two of these were also saliva positive. The highly leukotoxic JP2 strain was the most representative (60 % of the positive specimens; the samples from periodontal pockets and from saliva showed some ltx genotype for the same patient. Our experience suggests that this approach is suitable for a rapid and complete laboratory diagnosis for Aa infection.

  13. Improved Multiplex PCR Using Conserved and Species-Specific 16S rRNA Gene Primers for Simultaneous Detection of Actinobacillus actinomycetemcomitans, Bacteroides forsythus, and Porphyromonas gingivalis

    Tran, Simon Dangtuan; Rudney, Joel D.

    1999-01-01

    Among putative periodontal pathogens, Actinobacillus actinomycetemcomitans, Bacteroides forsythus, and Porphyromonas gingivalis are most convincingly implicated as etiological agents in periodontitis. Therefore, techniques for detection of those three species would be of value. We previously published a description of a multiplex PCR that detects A. actinomycetemcomitans and P. gingivalis. The present paper presents an improvement on that technique, which now allows more sensitive detection of all three periodontal pathogens. Sensitivity was determined by testing serial dilutions of A. actinomycetemcomitans, B. forsythus, and P. gingivalis cells. Primer specificity was tested against (i) all gene sequences from the GenBank-EMBL database, (ii) six A. actinomycetemcomitans, one B. forsythus, and four P. gingivalis strains, (iii) eight different species of oral bacteria, and (iv) supra- and subgingival plaque samples from 20 healthy subjects and subgingival plaque samples from 10 patients with periodontitis. The multiplex PCR had a detection limit of 10 A. actinomycetemcomitans, 10 P. gingivalis, and 100 B. forsythus cells. Specificity was confirmed by the fact that (i) none of our forward primers were homologous to the 16S rRNA genes of other oral species, (ii) amplicons of predicted size were detected for all A. actinomycetemcomitans, B. forsythus, and P. gingivalis strains tested, and (iii) no amplicons were detected for the eight other bacterial species. A. actinomycetemcomitans, B. forsythus, and P. gingivalis were detected in 6 of 20, 1 of 20, and 11 of 20 of supragingival plaque samples, respectively, and 4 of 20, 7 of 20, and 13 of 20 of subgingival plaque samples, respectively, from periodontally healthy subjects. Among patients with periodontitis, the organisms were detected in 7 of 10, 10 of 10, and 7 of 10 samples, respectively. The simultaneous detection of three periodontal pathogens is an advantage of this technique over conventional PCR assays. PMID

  14. Comparative activities of selected fluoroquinolones against dynamic populations of Actinobacillus pleuropneumoniae in an in vitro model of time-kill continuous culture experiment.

    Damte, Dereje; Lee, Seung-Jin; Yohannes, Sileshi B; Hossain, Md Akil; Suh, Joo-Won; Park, Seung-Chun

    2013-12-01

    The aim of the current study was to demonstrate and compare the impact of different pharmacokinetics of marbofloxacin, enrofloxacin and difloxacin on their antimicrobial effects, their killing and re-growth kinetics, and the population dynamics of Actinobacillus pleuropneumoniae clinical isolates in an in vitro dynamic model. Selected clinical isolates of A. pleuropneumoniae and three fluoroquinolones at a range of simulated AUC(24)/MIC ratios of multiple doses were investigated. At the same simulated AUC(24)/MIC ratios of the three fluoroquinolones, the killing re-growth profile and I(E) values (intensity of the antimicrobial effect) revealed strain- and fluoroquinolone-specific effects. For example, a 31% lower I(E) of difloxacin was observed in AppK5 (biofilm-former) than in AppK2 (biofilm-non-former) at the same AUC(24)/MIC ratio of 120 h. In addition, losses in A. pleuropneumoniae susceptibility of both strains by the three fluoroquinolones were observed. AUC(24)/MPC ratios of 20.89 and 39.81 for marbofloxacin, 17.32 and 19.49 for enrofloxacin and 31.62 and 60.25 for difloxacin were estimated to be protective against the selection of AppK2 and AppK5 strain mutants, respectively. Integration of these in vitro data with published pharmacokinetics revealed the inadequacy of the conventional clinical doses of the three drugs to attain the above protective values for minimum biofilm eradication concentration (MBEC) and concentration to prevent growth of 90% of the mutant subpopulation (MPC(90)). In conclusion, the results suggest optimising doses could suffice for resistant mutants control, while for biofilm-forming strains combination with biofilm-disrupting agents to reduce the MBEC to achieve AUC/MBEC ratios within the possible dosing regimens is desired. PMID:24139884

  15. Cloning and Expression of Actinobacillus pleuropneumoniae Gene Coding for TbpA and Development of an Indirect TbpA-ELISA

    LIANG Wang-wang; HE Qi-gai; CHEN Huan-chun; XU Di-ping; WU Rui; ZHANG Rong-rong

    2008-01-01

    This study presents the cloning and expression of gene encoding transferrin-binding protein A from Actinobacillus pleuropneumoniae in Escherichia coli expression system and the development of an indirect TbpA-ELISA. The gene coding TbpA was amplified from the A. pleuropneumoniae serotype 2 genome using polymerase chain reaction and cloned to pET-28b expression vector under the control of strong, inducible T7 promoter. The recombinant plasmid was expressed in E. coli BL21 (DE3). The expressed fusion protein was analyzed using SDS-PAGE and Western blotting. The diagnostic potential of recombinant TbpA (rTbpA) was evaluated through an antibody-detection indirect ELISA based on the purified rTbpA. The TbpA antibodies were detectable in mice on day 7 after vaccination with purified rTbpA protein or infection with A. pleuropneumoniae serotype 10 with the TbpA-based ELISA. In addition, the TbpA-ELISA was able to detect 12 serotyping rabbit antisera postinoculation (PI) with A. pleuropneumoniae 12 serotypes experimentally. The comparable result was obtained by detecting the 117 clinical serum samples using, respectively, the TbpA-ELISA and indirect hemagglutination test (IHA) based on multiplex antigen. The result indicates that the TbpA-ELISA was the more sensitive method compared with the Mix-IHA method because of its consistent presence in A. pleuropneumoniae serotypes. In conclusion, the conserved TbpA of A. pleuropneumoniae can be used for the development of a cross-serotype diagnostic method for the detection of antibodies against A. pleuropneumoniae.

  16. Nasal immunization with mannan-decorated mucoadhesive HPMCP microspheres containing ApxIIA toxin induces protective immunity against challenge infection with Actinobacillus pleuropneumoiae in mice.

    Li, Hui-Shan; Shin, Min-Kyoung; Singh, Bijay; Maharjan, Sushila; Park, Tae-Eun; Kang, Sang-Kee; Yoo, Han-Sang; Hong, Zhong-Shan; Cho, Chong-Su; Choi, Yun-Jaie

    2016-07-10

    The development of subunit mucosal vaccines requires an appropriate delivery system or an immune modulator such as an adjuvant to improve antigen immunogenicity. The nasal route for vaccine delivery by microparticles has attracted considerable interest, although challenges such as the rapid mucociliary clearance in the respiratory mucosa and the low immunogenicity of subunit vaccine still remain. Here, we aimed to develop mannan-decorated mucoadhesive thiolated hydroxypropylmethyl cellulose phthalate (HPMCP) microspheres (Man-THM) that contain ApxIIA subunit vaccine - an exotoxin fragment as a candidate for a subunit nasal vaccine against Actinobacillus pleuropneumoniae. For adjuvant activity, mucoadhesive thiolated HPMCP microspheres decorated with mannan could be targeted to the PRRs (pathogen recognition receptors) and mannose receptors (MR) of antigen presenting cells (APCs) in the respiratory immune system. The potential adjuvant ability of Man-THM for intranasal immunization was confirmed by in vitro and in vivo experiments. In a mechanistic study using APCs in vitro, it was found that Man-THM enhanced receptor-mediated endocytosis by stimulating the MR of APCs. In vivo, the nasal vaccination of ApxIIA-loaded Man-THM in mice resulted in higher levels of mucosal sIgA and serum IgG than mice in the ApxIIA and ApxIIA-loaded THM groups due to the specific recognition of the mannan in the Man-THM by the MRs of the APCs. Moreover, ApxIIA-containing Man-THM protected immunized mice when challenged with strains of A. pleuropneumoniae serotype 5. These results suggest that mucoadhesive Man-THM may be a promising candidate for a nasal vaccine delivery system to elicit systemic and mucosal immunity that can protect from pathogenic bacteria infection. PMID:27189136

  17. A novel Respiratory Health Score (RHS supports a role of acute lung damage and pig breed in the course of an Actinobacillus pleuropneumoniae infection

    Gerlach Gerald F

    2009-04-01

    Full Text Available Abstract Background Bacterial lung infections are a major cause of economic losses in the pig industry; they are responsible for approximately 50% of the antibiotics used in pigs and, therefore, also present an increasing concern to consumer protection agencies. In response to this changing market we investigated the feasibility of an old approach aimed at the breeding selection of more resistant pigs. As a first step in this direction we applied a new respiratory health score system to study the susceptibility of four different pig breeding lines (German Landrace, Piétrain, Hampshire, Large White towards the respiratory tract pathogen Actinobacillus (A. pleuropneumoniae. Results A controlled experimental aerosol infection with an A. pleuropneumoniae serotype 7 isolate was performed using 106 weaning pigs of defined breeding lines from the breeds German Landrace, Piétrain, Hamphire, and Large White. Pigs were clinically assessed on days 4 and 20 post infection following a novel scoring system, the Respiratory Health Score (RHS, which combines clinical, sonographic and radiographic examination results. The ranking on day 4 was significantly correlated with the ranking based on the pathomorphological Lung Lesion Score (LLS; Spearman Rank Correlation Coefficient of 0.86 [p Conclusion These results demonstrate that the RHS obtained from live pigs shows a highly significant correlation to the lung lesion score considered as a "gold standard". The correlation of the ranking at days 4 and 20 post infection implies that the course of disease is highly dependent on the acute lung damage. The different severity of signs among the tested pig breeding lines clearly suggests a genetic difference in the susceptibility of pigs to A. pleuropneumoniae infection.

  18. Padronização de três ELISAs polivalentes com lipopolissacarídeos de cadeia longa dos sorotipos 1 e 5, 2, 3 e 7 ou 10 e 12 de Actinobacillus pleuropneumoniae Standardization of three polyvalent ELISA based on long chain lipopolysaccharides of serotypes 1 and 5, 2, 3 and 7, or 10 and 12 of Actinobacillus pleuropneumoniae

    S.S. Kuchiishi

    2008-04-01

    Full Text Available Três ELISAs polivalentes baseados em lipopolissacarídeos de cadeia longa (LPS-CL foram estabelecidos para detectar anticorpos para todos os sorotipos prevalentes de Actinobacillus pleuropneumoniae. Foram testadas amostras provenientes do banco de soros de suínos experimentalmente inoculados com todos os sorotipos de A. pleuropneumoniae. Os ELISAs foram sensíveis à detecção de anticorpos contra todos os LPS-CL. Foram observadas reações cruzadas no ELISA polivalente produzido com os sorotipos 1 e 5, com anti-soros específicos para os sorotipos 9 e 11, pois os sorotipos 1, 9 e 11 apresentaram antígenos somáticos comuns. No polivalente com os sorotipos 2, 3 e 7, observaram-se reações com anti-soros dos sorotipos 4, 6 e 8, devido à presença de antígenos somáticos entre os sorotipos 3, 6 e 8 e entre os sorotipos 4 e 7. Amostras de soros de animais infectados com Mycoplasma hyopneumoniae, Mycoplasma flocculare e Haemophilus parasuis, agentes que acometem o sistema respiratório dos suínos, não apresentaram reações cruzadas com os antígenos baseados em LPS-CL.Three polyvalent ELISA based on long chain lipopolysaccharides (LC-LPS were established to detect all prevalent serotypes of Actinobacillus pleuropneumoniae. Samples from a serum bank of experimentally inoculated animals with all serotypes of A. pleuropneumoniae were tested. Antibodies specific to LC-LPS of each serotype were detected. Cross-reactions were observed in the polyvalent ELISA produced with serotypes 1 and 5, with specific antisera to serotypes 9 and 11 due to common somatic antigens presence in serotypes 1, 9, and 11. In the polyvalent with serotypes 2, 3 and 7 reactions were observed with antisera of serotypes 4, 6, and 8, due to the presence of somatic antigens in serotypes 3, 6, and 8 and serotypes 4 and 7. Experimentally infected animals with respiratory agents of swine Mycoplasma hyopneumoniae, Mycoplasma flocculare, and Haemophilus parasuis did not present

  19. Association between infection of different strains of Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans in subgingival plaque and clinical parameters in chronic periodontitis

    WU Yan-min; YAN Jie; CHEN Li-li; GU Zhi-yuan

    2007-01-01

    Objective: The aim of this study was to investigate subgingival infection frequencies ofPorphyromonas gtngivalis and Actinobacillus actinomycetemcomitans strains with genetic variation in Chinese chronic periodontitis (CP) patients and to evaluate its correlation with clinical parameters. Methods: Two multiplex polymerase chain reaction (PCR) assays were developed to detect the 16SrDNA, collagenase (prtC) and fimbria (fimA) genes of P. gingivalis and the 16SrDNA, leukotoxin (lktA) and fimbria-associated protein (lap) genes ofA. actinomycetemcomitans in 60 sulcus samples from 30 periodontal healthy subjects and in 122 subgingival plaque samples from 61 patients with CP. The PCR products were further T-A cloned and sent for nucleotide sequence analysis. Results: The 16SrDNA, prtC andfimA genes ofP. gingivalis were detected in 92.6%, 85.2% and 80.3% of the subgingival plaque samples respectively, while the 16SrDNA, lktA andfap genes ofA. actinomycetemcomitans were in 84.4%,75.4% and 50.0% respectively. Nucleotide sequence analysis showed 98.62%~100% homology of the PCR products in these genes with the reported sequences. P. gingivalis strains with prtC+/fimA+ and A. actinomycetemcomitans with lktA+ were predominant in deep pockets (>6 mm) or in sites with attachment loss ≥5 mm than in shallow pockets (3~4 mm) or in sites with attachment loss ≤2 mm (P<0.05). P. gingivalis strains with prtC+/fimA+ also showed higher frequency in gingival index (GI)=3than in GI= 1 group (P<0.05). Conclusion: Infection of P. gingivalis with prtC+/fimA+ and A. actinomycetemcomitans with lktA+correlates with periodontal destruction of CP in Chinese. Nonetheless P. gingivalis fimA, prtC genes and A. actinomycetemcomitans IktA gene are closely associated with periodontal destruction, while A. actinomycetemcomitansfap gene is not.

  20. Reação em Cadeia da Polimerase (PCR baseada no gene cpx para detecção de Actinobacillus pleuropneumoniae em suínos natural e experimentalmente infectados Polymerase Chain Reaction (PCR based on the cpx gene for detection of Actinobacillus pleuropneumoniae in natural and experimentally infected pigs

    Karina Koerich de Souza

    2008-10-01

    Full Text Available A pleuropneumonia suína é uma das mais importantes doenças respiratórias dos suínos, estando presente em todos os países produtores. Para o controle e o monitoramento da pleuropneumonia, é necessário o desenvolvimento de métodos rápidos e acurados de diagnóstico. Com o objetivo de validar a técnica da PCR, baseada no gene cpx de Actinobacillus pleuropneumoniae, em suínos sabidamente positivos, primeiramente foi realizada inoculação experimental com amostras de A. pleuropneumoniae sorotipo 5B e coletadas amostras por meio de suabe de tonsila, biópsia de tonsila e sangue para realização da técnica de PCR, isolamento bacteriológico e teste de ELISA, respectivamente. Posteriormente, estas técnicas foram aplicadas em suínos naturalmente infectados, em três rebanhos com diferentes situações sanitárias quanto à apresentação clínica da doença. De cada rebanho, foram analisados cinco grupos de suínos com idades diferentes, sendo coletado de cada animal biópsia de tonsila para isolamento bacteriológico e PCR e sangue para determinação do perfil sorológico. Os resultados obtidos na inoculação experimental confirmaram que, mesmo com o estabelecimento da infecção comprovada pelo isolamento bacteriológico, após o período de 45 dias, não foi possível detectar o agente pela técnica de PCR. Em animais naturalmente infectados, a técnica de PCR apresentou maior sensibilidade quando comparado com o isolamento. A associação entre PCR e ELISA demonstrou ser uma boa alternativa para definir a situação sanitária do rebanho quanto à infecção por A. pleuropneumoniae.Swine pleuropneumonia is one of the most important pig respiratory diseases and has been found in all producer countries. For control and monitoring of pleuropneumonia, it is necessary the development of fast and specific methods of diagnosis. To validate PCR based on the cpx gene of Actinobacillus pleuropneumoniae in positive pigs, an experimental

  1. Estudios hematológicos y patológicos comparativos de cerdos inoculados con un aislado de campo y el serotipo 5 ATCC de Actinobacillus pleuropneumoniae Comparative hematological and pathological study of inoculated pigs with a field isolate and an ATCC serotype 5 of Actinobacillus pleuropneumoniae

    D Muñoz

    2010-01-01

    Full Text Available Se realizó una inoculación experimental de A. pleuropneumoniae utilizando un aislado de campo y una cepa de referencia ATCC serotipo 5, para lo cual se utilizaron tres grupos de animales (n = 15 para cada grupo. El grupo 1 (G1 fue inoculado con medio estéril, el grupo (G2 con serotipo 5 ATCC y el grupo 3 (G3 fue inoculado con un aislado de campo (418/07. Los resultados mostraron diferencias significativas (P ≤ 0,05 en el recuento de leucocitos totales entre el grupo G1 v/s G2 y G1 v/s G3 y los grados de las lesiones pulmonares totales evidenciaron diferencias estadísticamente significativas (P ≤ 0,05 entre los tres grupos de estudio. Las lesiones histopatológicas pulmonares mostraron diferencias estadísticas relevantes sólo entre G1 y G3 (P ≤ 0,05. En este trabajo se verifican diferencias importantes del comportamiento entre el aislado de campo y el serotipo 5 ATCC, sobre los cambios hematológicos y las lesiones macroscópicas e histopatológicas ocasionadas por ellos, lo cual podría indicar una mayor virulencia y patogenicidad del aislado nacional. Se espera en un futuro próximo serotipificar este aislado nacional de App.An experimental inoculation of Actinobacillus pleuropneumoniae (App was carried out with a field isolate and an ATCC serotype 5. Three groups of 15 pigs each were used. Group 1 (G1 was the control group inoculated with sterile media, Group 2 was inoculated with the serotype 5 ATCC, and Group 3 (G3 was inoculated with a field isolate (418/07. The results showed statistically significant differences (P ≤ 0.05 in the total leukocytes count between G1 v/s G2 and G1 v/s G3. The total macroscopic lung lesions scores were statistically different among the 3 groups (P ≤ 0.05. However, statistical difference was found only between G1 and G3 in the histopathological lung lesions (P ≤ 0.05. This work shows a clear difference in the hematological changes and the macroscopic and histopathological lesions between the

  2. Occurrence of Actinobacillus actinomycetemcomitans in patients with chronic periodontitis, aggressive periodontitis, healthy subjects and children with gingivitis in two cities of the state of São Paulo, Brazil Ocorrência de Actinobacillus actinomycetemcomitans em pacientes com periodontite crônica, periodontite agressiva, pessoas saudáveis e crianças com gengivite em duas cidades do Estado de São Paulo, Brasil

    Elerson Gaetti Jardim Júnior

    2006-06-01

    Full Text Available The aim of this study was to determine the frequency of isolation of Actinobacillus actinomycetemcomitans (Aa in 100 patients with chronic periodontitis, 14 patients with aggressive periodontitis, 142 pre-school children with gingivitis and 134 periodontally healthy subjects. Samples of subgingival plaque were taken using sterilized paper points introduced into periodontal pockets or gingival crevice for 60 seconds and inoculated on TSBV agar, which was incubated under anaerobiosis at 37ºC, for 4 days. Microbial identification was performed through biochemical methods and morphocellular and morphocolonial analysis. Aa was detected in 40.3% of healthy subjects, 68% of patients with chronic periodontitis, 92.86% of patients with aggressive periodontitis and 40.14% of children with gingivitis. The rate of recovery of Aa in the tested human groups proved to be higher than previously reported and in agreement with participation of this facultative anaerobe as a member of native microbiota of the periodontium and its relation with aggressive and chronic periodontitis in Brazil.Avaliou-se a ocorrência de Actinobacillus actinmycetemcomitans (Aa em pacientes 100 pacientes com periodontite crônica, 14 com doença periodontal agressiva, 142 crianças com gengivite em idade pré-escolar e 134 indivíduos adultos saudáveis. Amostras de placa subgengival foram coletadas usando cones de papel estéreis introduzidos nas bolsas periodontais ou no sulco gengival por 60 segundos e inoculadas em ágar TSBV, que foram incubadas em anaerobiose a 37ºC, por 4 dias. A identificação microbiana foi realizada através de análises bioquímicas, morfocelulares e morfocoloniais. Aa foi detectado em 40,3% de indivíduos saudáveis, 68% de pacientes com periodontite crônica, 92,86% de pacientes com periodontite agressiva e 40,14% das crianças com gengivite. A taxa de ocorrência de Aa nos grupos testados provou ser mais alta do que a previamente descrita na literatura

  3. PCR specific for Actinobacillus pleuropneumoniae serotype 3

    Zhou, L.; Jones, S.C.P.; Angen, Øystein;

    2008-01-01

    , but the method has liminations, for example, cross-reactions between serotypes 3, 6, and 8. This study describes the development of a serotype 3-specific PCR, based on the capsule locus, which can be used in a multiplex format with the organism's specific gene apxIV. The PCR test was evaluated on 266...

  4. 伴放线放线杆菌cdtB基因克隆及其表达蛋白的体外生物学活性检测%Gene cloning of cdtB from Actinobacillus actinomycetemcomitans and bioactivity test of the recombinant protein CdtB in vitro

    李璐; 段君兰; 王晓茜; 杨迷芳; 徐艳

    2010-01-01

    Objective To construct the prokayotic expression vector pET-15b-cdtB containing the cdtB gene from Actinobacillus actinomycetemcomitans (Aa) and to test the bioactivity of this recombinant CdtB in vitro. Methods The toxic cytolethal distending toxin (CDT) subunit encoding gene cdtB was amplified by PCR. Through restriction endonuclease digestion,gene cdtB and vector pET-15b were ligated to form pET-15b-cdtB expression system which was transformed into competent cells Escherichia coli BL21 ( DE3 ). Protein expression was induced by isopropyl-beta-D-thiogalactoside and examined by sodium dodecyl sulfate-pelyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Supercoiled plasmid pET-32a DNA was incubated with purified recombinant CdtB protein in vitro to view any changes in the electrophoretic mobility of the plasmid pET-32a DNA band. Results PCR testing results of pET-15b-cdtB transformed cells demonstrated that all strains contained cdtB gene. The DNA sequence was blast with cdtB gene from GenBank and 99% homology was obtained. Both of SDS-PAGE and Western blotting confirmed that recombinant CdtB was obtained. After incubated with the purified recombinant CdtB in vitro, the supercoiled plasmid pET-32a DNA was observed relaxing by 1% ngarose gel electrophoresis test. Conclusions The recombinant plasmid pET-15b-cdtB was successfully constructed and the recombinant CdtB protein which has the Dnase Ⅰ -like activity was obtained.%目的 体外构建伴放线放线杆菌(Actinobacillus actinomycetemcomitans,Aa)CdtB蛋白的原核表达载体并诱导其表达,通过变性、复性获得有Ⅰ型脱氧核糖核酸酶(deoxyribonuclease Ⅰ,DNase Ⅰ)样活性的重组CdtB蛋白,为进一步研究AaCdtB的功能以及Aa细胞致死性扩张毒素三聚体全毒素在牙周炎发生、发展过程中的分子致病机制奠定基础.方法 以AaATCC29522基因组DNA为模板,采用聚合酶链反应(PCR)法获得cdtB基因,经双酶切、连接的定向克隆

  5. Actinobacillus minor sp nov, Actinobacillus porcinus sp nov, and Actinobacillus indolicus sp nov, three new V factor-dependent species from the respiratory tract of pigs

    Møller, Kristian; Fussing, V.; Grimont, P.A.D.; Paster, B.J.; Dewhirst, F.E.; Kilian, M.

    1996-01-01

    showed that the Minor group acid taxa C, D plus E, and F are distinct phylogenetic groups that are separate from each other and from other members of the family Pasteurellaceae, On the basis of these results, three new species, corresponding to the Minor group, taxa D plus E, and taxon F, are proposed...

  6. Construction of the Gene Deleted Attenuated Mutant Strain apxIIC -/kanr + of Actinobacillus pleuropneumoniae%胸膜肺炎放线杆菌apxlIC7kanr+基因缺失减毒株的构建

    李建; 曹三杰; 文心田; 黄小波; 都启晶; 张明; 余慧

    2012-01-01

    The recombinant transfer vector pBSKA was electroporated into parent strain Actinobacillus pleuropneumoniae serovar 7 (APP -7 ) strain WF83. Product of the electroporation was plated onto TSB agar containing kanamyeine (Kan). After 2 days the recombinant strains were selected. Resistance of kanamycine experiment confirmed that mutant strain can counteract kanamycine. Dependence experiment of NAD confirmed that mutant strain needed NAD in growth. Identification of PCR confirmed that complete apxIIC gene was substitute for kanamycine resistance gene and there was no presence of pBSKA. Hemolytic experiment confirmed that mutant strain had no ability of haemolysis. Cytotoxicity test confirmed that mutant strain had no cytotoxieity. Safety experiment of injected mice confirmed that eytotoxicity and haemolysis of mutant was attenuated significantly so that mutant was safe to mice. Experiment of genetic stability confirmed that kanamycine resistance of mutant was stable 30 successive generations in vitro and 10 generations in vivo. All of the above - mentioned tests indicated that the gene deleted attenuated strain was constructed successfully, which provided certain basis for further genetic live vaccine research with mutant strain.%重组转移载体pBSKA通过电转化导入亲本菌胸膜肺炎放线杆菌血清7型(APP-7)WF83株,电转化后的产物涂布于TSB/Kan平板,2d获得突变株。卡那霉素抗性实验证实突变株有卡那霉素抗性;NAD依赖性实验证实突变株依赖NAD生长;PCR鉴定证实了卡那霉素抗性基因置换了apxlIC基因,并证实突变株中无pBSKA质粒的存在;溶血活性实验证实突变株完全失去了溶血活性;细胞毒性实验证实突变株的细胞毒性完全丧失;对小鼠的安全性实验证实突变株的毒力显著减弱,突变株对小鼠是安全的;遗传稳定性实验证实,突变株在体外连续传30代和在体内传10代均不会发生卡

  7. Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences.

    Dewhirst, F E; Paster, B J; Olsen, I; Fraser, G J

    1992-03-01

    Virtually complete 16S rRNA sequences were determined for 54 representative strains of species in the family Pasteurellaceae. Of these strains, 15 were Pasteurella, 16 were Actinobacillus, and 23 were Haemophilus. A phylogenetic tree was constructed based on sequence similarity, using the Neighbor-Joining method. Fifty-three of the strains fell within four large clusters. The first cluster included the type strains of Haemophilus influenzae, H. aegyptius, H. aphrophilus, H. haemolyticus, H. paraphrophilus, H. segnis, and Actinobacillus actinomycetemcomitans. This cluster also contained A. actinomycetemcomitans FDC Y4, ATCC 29522, ATCC 29523, and ATCC 29524 and H. aphrophilus NCTC 7901. The second cluster included the type strains of A. seminis and Pasteurella aerogenes and H. somnus OVCG 43826. The third cluster was composed of the type strains of Pasteurella multocida, P. anatis, P. avium, P. canis, P. dagmatis, P. gallinarum, P. langaa, P. stomatis, P. volantium, H. haemoglobinophilus, H. parasuis, H. paracuniculus, H. paragallinarum, and A. capsulatus. This cluster also contained Pasteurella species A CCUG 18782, Pasteurella species B CCUG 19974, Haemophilus taxon C CAPM 5111, H. parasuis type 5 Nagasaki, P. volantium (H. parainfluenzae) NCTC 4101, and P. trehalosi NCTC 10624. The fourth cluster included the type strains of Actinobacillus lignieresii, A. equuli, A. pleuropneumoniae, A. suis, A. ureae, H. parahaemolyticus, H. parainfluenzae, H. paraphrohaemolyticus, H. ducreyi, and P. haemolytica. This cluster also contained Actinobacillus species strain CCUG 19799 (Bisgaard taxon 11), A. suis ATCC 15557, H. ducreyi ATCC 27722 and HD 35000, Haemophilus minor group strain 202, and H. parainfluenzae ATCC 29242. The type strain of P. pneumotropica branched alone to form a fifth group. The branching of the Pasteurellaceae family tree was quite complex. The four major clusters contained multiple subclusters. The clusters contained both rapidly and slowly evolving

  8. Isolation of Actinobacillus pleuropneumoniae serotype 2 by immunomagnetic separation

    Angen, Øystein; Heegaard, Peter M. H.; Lavritsen, D.T.;

    2001-01-01

    ) using immunomagnetic beads (Dynabeads(R)) was developed for isolation of A. pleuropneumoniae serotype 2 from pure cultures and from heterogeneous suspensions. Different coating and washing procedures were evaluated in pure and mixed cultures using polyclonal (PAb) and monoclonal antibodies. The highest...... reisolation yield was achieved when the beads were coated with 1.5 mug PAb IgG/10(7) beads. After washing the beads for four times 9-24% of the bacteria could be reisolated depending on the amount of IgG attached to the beads and the number of beads used. The recovery was increased to 19-61% when only two...... in this heterogeneous suspension. No significant difference was observed when comparing the recovery of A. pleuropneumoniae from pure culture, from mixed cultures and from artificially inoculated tonsils. From 12 pigs inoculated with an aerosol of A. pleuropneumoniae serotype 2 the bacterium could not be detected from...

  9. Detection of an Actinobacillus pleuropneumoniae serotype 2 lipopolysaccharide (LPS) variant

    Stenbaek, E.I.; HovindHaugen, K.

    1996-01-01

    linear repeating pentasaccharide units with an O-acetyl group linked to a glucose unit. A monoclonal antibody (MAb 102-G02) directed against A. pleuropneumoniae serotype 2 was characterized in enzyme linked immunosorbent assay (ELISA) and in sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS...

  10. An Unusual Occurrence of Actinobacillosis in Heifers and Cows in A Dairy Herd in Tehran suburb-Iran

    Atyabi, N.,

    2010-07-01

    Full Text Available An unusual occurrence of actinobacillosis was diagnosed in 4 heifers aged 8-15 months and 2 cattle in a dairy herd with 190 Iranian Holstein breed. Anorexia, dysphagia, drooling of normal or foodtinged saliva and presence of warts-like lesions on the dorsal surface of tongue shaft were observed in a 15-month-old heifer without showing protrusion of tongue or presenting woody tongue and no involvement of either sulcus lingualis or tongue base. In addition to tongue, soft tissues of oral cavity and pharyngeal region including lymph nodes, salivary glands and tonsils were contained multiple whitish nodules. Histopathologically, typical pyogranulomas of actinobacillosis contained radiating eosinophilic clubs surrounded by many neutrophils were diagnosed. Actinobacillus lignieresii was isolated from the lesions in pure culture. Clinical examination of other animals revealed the presence of different degrees of granulomatous abscesses in soft tissues including skin around mandibles in at least 3 heifers aged 8-11 months and 2 cattle. Due to 4 recent droughty years feeding the heifers, dry cows and low milk producing cattle by cheap oat and wheat straw mixed with plant awns could be the cause of this event.

  11. Construction of An Activation of Toxin 1 Gene C (△apxIC) Mutant of Actinobacillus pleuropneumoniae Gomphosised Outer Membrane Protein P2 Gene(ompP2) of Haemophilus parasuis%猪传染性胸膜肺炎放线杆菌缺失毒素1激活基因C(ApxIC)同时嵌合副猪嗜血杆菌外膜蛋白P2基因(ompP2)复合突变株的构建

    刘琼; 龚雨恒; 王国镔; 文心田; 黄小波; 曹三杰

    2012-01-01

    猪胸膜肺炎放线杆菌(Actinobacillus pleuropneumoniae,APP)以及副猪嗜血杆菌(Haemophilus parasuis,Hps)是引起猪发病的2种病菌.本研究以APP血清5型分离株(SW1)为基础材料,通过构建重组转移载体pBOSK△IC-1和pBOSK△IC/ompP2,构成卡那霉素抗性基因(Kanr)和枯草芽胞杆菌(Bacillus subtilis)的果聚蔗糖酶基因(sacB)的正负双向筛选表达盒,并筛选获得SW1株缺失毒素Ⅰ的激活基因C(Apx(I)C),同时嵌合Hps外膜蛋白P2基因(ompP2)的复合突变株SW1 △ApxIC/ompP2.该突变株经鉴定,与SW1亲本株相比,其缺失了大小为475 bp的apxIC基因,同时嵌合有大小为1 107bp的Hps ompP2基因,其基本生长特性与亲本株(SW1)没有显著区别;同时在体外,连续传代10代之后缺失的apxIC基因不会发生回复突变,嵌入的ompP2基因仍能够稳定遗传.本研究成功构建了猪传染性胸膜肺炎放线杆菌缺失apxIC基因并嵌合有Hps ompP2基因复合突变株,为今后研究APP和Hps新型二联疫苗打下基础.%Actinobacillus pkuropnewnoniae (APP) and Haemophilus parasuis (Hps) are the bacteria of causing porcine disease. In this study, We constructed two recombinant vectores pBOSKA/C-1 and pBOSKAIC/ompP2 using APP5 (SW1) strain as a template, and the two recombinant vectores were used for plus-minus screening system with the basis of Kanr and sacB. The mutant strain, named SW1 A apxICIompP2, was obtained, which was deleted toxin I gene C (apxIC) in SW1 and gotnphosised outer membrane protein P2 Hps gene {ompPZ). The mutant missed a size of 475 bp apxIC gene but was inserted a size of 1107 bp Hps ompP2 gene compared with the parental strain SW1. No reverse mutation of apxlC gene was observed during after 10 generations, while Hps ompP2 gene still had a good genetic stability in vitro, the mutant strain had no significant difference of he basic growth characteristics with the parental strain (SW1). The results confirmed that an \\apxlC mutant of A

  12. Microevolution and Patterns of Dissemination of the JP2 Clone of Aggregatibacter (Actinobacillus) actinomycetemcomitans

    Haubek, Dorte; Poulsen, Knud; Kilian, Mogens

    2007-01-01

    belonging to the JP2 clone had a number of point mutations, particularly in the pseudogenes hbpA and tbpA. Characteristic mutations allowed isolates from individuals from the Mediterranean area and from West Africa, including the Cape Verde Islands, to be distinguished. The patterns of mutations indicate...... that the JP2 clone initially emerged as a distinct genotype in the Mediterranean part of Africa approximately 2,400 years ago and subsequently spread to West Africa, from which it was transferred to the American continents during the transatlantic slave trade. The sustained exclusive colonization...

  13. Selective media for the isolation of Actinobacillus pleuropneumoniae from the pig

    Vidić Branka M.

    2004-01-01

    Full Text Available Selective media were developed and evaluated for isolation of A. pleuropneumoniae from pig tonsils. Samples were obtained from four pig herds with a clinical history of pleuropneumonia. For isolation of A. pleuropneumoniae 93 pig tonsils were collected at slaughter. Each sample was streaked on to four different selective media (modified PPLO agar (mPPLO Brain-Heart agar (BH, Columbia agar (CA, Miller-Hinton chocolate agar (MHCA containing different combinations of antibiotics, NAD and nystatin. The selectivity of nutritive media is conditioned by the content of antibiotics, as well as by the type of medium used. Mean isolation rate of A. pleuropneumoniae in the investigated herds, was 17.2%. The best results were obtained using PPLO2 agar, 20.4%. The other media supplemented with the mentioned antibiotics gave satisfactory results.

  14. Serological characterization of Actinobacillus pleuropneumoniae biotype 1 strains antigenically related to both serotypes 2 and 7

    Nielsen, R.; Andresen, Lars Ole; Plambeck, Tamara

    1996-01-01

    ). Immunodiffusion confirmed the antigenic relationship with serotype 2 and further demonstrated an antigenic relationship with strain WF83 (reference strain of serotype 7). SDS-PAGE with LPS from strains 1536, 4226, WF83 and strain 7317 (representative of the 9 isolates examined) showed that strains WF83 and 7317...

  15. Evaluation and application of ribotyping for epidemiological studies of Actinobacillus pleuropneumoniae in Denmark

    Fussing, V.; Barfod, Kristen; Nielsen, R.;

    1998-01-01

    discriminatory power was between 0.85-0.89. The relatively low discriminatory power was caused by four predominant types, containing 61% of the isolates. The typing system was applied in studies of routes of infection of specific pathogen-free (SPF) pig herds and included 112 strains of A. pleuropneumoniae...

  16. Clinical and Pathological Changes in Rams Experimentally Infected with Actinobacillus seminis and Histophilus somni

    Valéria S. Moustacas; Silva, Teane M. A.; Luciana F. Costa; Custódio A. Carvalho Júnior; Santos, Renato L.; Tatiane A. Paixão

    2014-01-01

    Infectious epididymitis is considered a major cause of economic losses for the sheep industry worldwide. This study aimed to investigate clinical and pathological changes associated with experimental infections with A. seminis and H. somni in rams. Twenty rams of age 18 to 24 months were infected by intraepididymal inoculation of A. seminis (n = 10) and H. somni (n = 10). Rams were weekly examined and biological samples were collected during six weeks. All rams inoculated with A. seminis and ...

  17. Clinical and pathological changes in rams experimentally infected with Actinobacillus seminis and Histophilus somni.

    Moustacas, Valéria S; Silva, Teane M A; Costa, Luciana F; Carvalho Júnior, Custódio A; Santos, Renato L; Paixão, Tatiane A

    2014-01-01

    Infectious epididymitis is considered a major cause of economic losses for the sheep industry worldwide. This study aimed to investigate clinical and pathological changes associated with experimental infections with A. seminis and H. somni in rams. Twenty rams of age 18 to 24 months were infected by intraepididymal inoculation of A. seminis (n = 10) and H. somni (n = 10). Rams were weekly examined and biological samples were collected during six weeks. All rams inoculated with A. seminis and 80% inoculated with H. somni became infected. The recovery of bacteria was possible in semen and urine samples and tissues in both experimental groups. Clinically, there were a decrease in testicular consistency and an increase in measures of the left epididymis tails in both experimental groups. The main gross changes were observed in the reproductive tract. Microscopically, the main lesions were inflammatory changes in the genitourinary tract and testicular degeneration. A. seminis and H. somni were able to colonize several organs of the genitourinary tract in rams, being indistinguishable by clinical exam, necropsy or histopathology. For differential diagnosis, it is important to use diagnostic techniques for direct confirmation of the etiologic agent. PMID:24592151

  18. Comparison of high and low virulence serotypes of Actinobacillus pleuropneumoniae by quantitative real-time PCR

    Schou, Kirstine Klitgaard; Angen, Øystein; Boye, Mette

    high virulence while serotype 6 strains are normally found to be less pathogenic. To gain an understanding of the differential virulence of serotype 2 and 6, the expression of a panel of Ap genes during infection of porcine epithelial lung cells (SJPL) were examined by quantitative real-time PCR (qPCR...... be important for early establishment of the bacteria in the host were examined by qPCR. The genes examined were apfA, coding for a subunit of Type IV pili, kdsB coding for a gene involved in lippopolysacceride biosynthesis, and pgaB which is involved in biofilm formation, all three believed to be...... normalization of the qPCR data. Preliminary results showed that in both serotype 2 and serotype 6, the toxin producing gene apxIV was the most highly expressed of the investigated genes. The major difference observed between the two serotypes was that apfA, involved in type IV vili production, was significantly...

  19. Genome-wide annotation of porcine microRNA genes and transcriptome profiling during Actinobacillus infection

    Nielsen, Mathilde

    MicroRNAs are small single stranded non-coding RNA molecules which contributes to the regulation of gene expression by primarily binding to the 3´end of protein coding mRNA, hereby inhibiting the translation process or promting degradation of the mRNA. The main focus of this PhD project was to...

  20. Putative biomarkers for evaluating antibiotic treatment: an experimental model of porcine Actinobacillus pleuropneumoniae infection

    Lauritzen, B.; Lykkesfeldt, J.; Skaanild, M.T.;

    2003-01-01

    animals received a single dose of either danofloxacin (2.5 mg/kg) or tiamulin (10 mg/kg). To test the discriminative properties of the biomarkers, the dosage regimens were designed with an expected difference in therapeutic efficacy in favour of danofloxacin. Accordingly, the danofloxacin-treated pigs...... recovered clinically within 24h after treatment, whereas tiamulin-treated animals remained clinically ill until the end of the study, 48 h after treatment. A similar Picture was seen for the biomarkers of infection. During the infection period, plasma C-reactive protein (CRP), interleukin-6 and haptoglobin...... increased, whereas plasma zinc, ascorbic acid and alpha-tocopherol decreased. In the danoffoxacin-treated animals, CRP, interleukin-6, zinc, ascorbic acid and alpha-tocopherol reverted significantly towards normalisation within 24h of treatment. In contrast, signs of normalisation were absent (CRP, zinc and...

  1. Actinobacillus pleuropneumoniae osteomyelitis in pigs demonstrated by fluorescent in situ hybridization

    Jensen, Tim Kåre; Boye, Mette; Hagedorn-Olsen, T.;

    1999-01-01

    of A. pleuropneumoniae in formalin-fixed tissue was performed to verify the association of A. pleuropneumoniae with the bone and joint lesions. By in situ hybridization A. pleuropneumoniae was demonstrated as multiple microcolonies or single cells dispersed in focal fibrinonecrotizing pleuropneumonia...

  2. Early-onset periodontitis in Morocco is associated with the highly leukotoxic clone of Actinobacillus actinomycetemcomitans

    Haubek, Dorte; Ennibi, O.-K.; Poulsen, Knud; Poulsen, Sven; Benzart, N; Kilian, Mogens

    2001-01-01

    schoolchildren. Of 217 plaque samples, 131 (60.4%) were culture-positive for A. actinomycetemcomitans. A total of 19 of these isolates had a 530-bp deletion in the leukotoxin promoter region characteristic of the JP2 clone. A strong association between the presence of A. actinomycetemcomitans with the 530-bp...

  3. An atypical biotype I Actinobacillus pleuropneumoniae serotype 13 is present in North America

    Perry, Malcolm B.; Angen, Øystein; MacLean, Leann L.;

    2012-01-01

    analysis of the capsular polysaccharide (CPS) and lipopolysaccharide (LPS) of a representative strain revealed that the CPS is almost identical to that of the reference strain of serotype 13, having a slightly higher degree of glycose O-acetylation. However, it produces an O-PS within the LPS antigenically...... and structurally identical with that of the reference strain of A. pleuropneumoniae serotype 10. The O-PS was characterized as a homopolymer of 1,2 linked β-d-galactofuranosyl residues, a structure unrelated to that of the O-PS produced by the reference strain of serotype 13. Strains from Canada and...

  4. Complete Genome Sequence of MIDG2331, a Genetically Tractable Serovar 8 Clinical Isolate of Actinobacillus pleuropneumoniae

    Bossé, JT; Chaudhuri, RR; Li, Y; Leanse, LG; Fernandez Crespo, R; Coupland, P; Holden, MT; Bazzolli, DM; Maskell, DJ; Tucker, AW; Wren, BW; Rycroft, AN; Langford, PR

    2016-01-01

    Wellcome Trust provided funding to Paul Coupland and Matthew Holden under grant number 098051. Biotechnology and Biological Sci- ences Research Council (BBSRC) provided funding to Janine T. Bosse, Roy R. Chaudhuri, Yanwen Li, Leon G. Leanse, Roberto Fernandez Cre- spo, Paul Coupland, Matthew Holden, Denise Mara Soares Bazzolli, Dun- can J. Maskell, Dan Tucker, Brendan W. Wren, Andrew N. Rycroft, Paul R. Langford, and BraDP1t Consortium under grant numbers BB/ G020744/1, BB/G019177/1, BB/G0192...

  5. Cytoplasmic N-Glycosyltransferase of Actinobacillus pleuropneumoniae Is an Inverting Enzyme and Recognizes the NX(S/T) Consensus Sequence*

    Schwarz, Flavio; Fan, Yao-Yun; Schubert, Mario; Aebi, Markus

    2011-01-01

    N-Linked glycosylation is a frequent protein modification that occurs in all three domains of life. This process involves the transfer of a preassembled oligosaccharide from a lipid donor to asparagine side chains of polypeptides and is catalyzed by the membrane-bound oligosaccharyltransferase (OST). We characterized an alternative bacterial pathway wherein a cytoplasmic N-glycosyltransferase uses nucleotide-activated monosaccharides as donors to modify asparagine residues of peptides and pro...

  6. Qualitative and quantitative determination of enterobacterial common antigen (ECA) with monoclonal antibodies: expression of ECA by two Actinobacillus species.

    Böttger, E C; Jürs, M; Barrett, T; Wachsmuth, K; Metzger, S.; Bitter-Suermann, D

    1987-01-01

    The presence and quantity of the enterobacterial common antigen (ECA) in several species belonging to the family Enterobacteriaceae as well as to other gram-negative families were determined by a solid-phase enzyme-linked immunosorbent assay system and Western blotting by using mouse monoclonal antibodies specific for ECA. Except for Erwinia chrysanthemi, previously known to be an exception, all species known or presumed to belong to Enterobacteriaceae produced ECA (89 of 90 species). Most sp...

  7. DEVELOPMENT AND EVALUATION OF A SELECTIVE AND INDICATIVE MEDIUM FOR ISOLATION OF ACTINOBACILLUS-PLEUROPNEUMONIAE FROM TONSILS

    Jakobsen, Marianne; Nielsen, Jens

    In order to isolate ActinobacillIus pleuropneumoniae from mixed bacterial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar (S-MBA) using a set of 25 strains of A. pleuropn......In order to isolate ActinobacillIus pleuropneumoniae from mixed bacterial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar (S-MBA) using a set of 25 strains of A...

  8. DEVELOPMENT AND EVALUATION OF A SELECTIVE AND INDICATIVE MEDIUM FOR ISOLATION OF ACTINOBACILLUS-PLEUROPNEUMONIAE FROM TONSILS

    Jakobsen, Marianne; Nielsen, Jens

    1995-01-01

    In order to isolate ActinobacillIus pleuropneumoniae from mixed bacterial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar (S-MBA) using a set of 25 strains of A. pleuropn......In order to isolate ActinobacillIus pleuropneumoniae from mixed bacterial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar (S-MBA) using a set of 25 strains of A...

  9. Utilization of CO2 fixating bacterium Actinobacillus succinogenes 130Z for simultaneous biogas upgrading and bio-succinic acid production

    Gunnarsson, Ingólfur Bragi; Alvarado-Morales, Merlin; Angelidaki, Irini

    2014-01-01

    Biogas is an attractive renewable energy carrier. However, it contains CO2 which limits certain applications of biogas. Here we report a novel approach for removing CO2 from biogas and capturing it as a biochemical through a biological process. This approach entails converting CO2 into bio-succin...

  10. Evaluation of a multiplex PCR test for simultaneous identification and serotyping of Actinobacillus pleuropneumoniae serotypes 2, 5, and 6

    Jessing, Stine Graakjær; Angen, Øystein; Inzana, Tomas J.

    2003-01-01

    6 were combined with the already existing species-specific primers used in a PCR test based on the omlA gene. The PCR test was evaluated with serotype reference strains of A. pleuropneumoniae as well as 182 Danish field isolates previously serotyped by latex agglutination or immunodiffusion. For all...... cross-reacted by the latex agglutination test were of serotype 2, 5, or 6. Determination of the serotype by PCR represents a convenient and specific method for the serotyping of A. pleuropneumoniae in diagnostic laboratories....

  11. Transcriptional Portrait of Actinobacillus pleuropneumoniae during Acute Disease - Potential Strategies for Survival and Persistence in the Host

    Schou, Kirstine Klitgaard; Rundsten, Carsten Friis; Jensen, Tim Kåre; Angen, Øystein; Boye, Mette

    2012-01-01

    results suggested that A. pleuropneumoniae is using various strategies to increase its fitness, such as applying Na+ pumps as an alternative way of gaining energy. Furthermore, the transcriptional data provided potential clues as to how A. pleuropneumoniae is able to circumvent host immune factors and...... survive within the hostile environment of host macrophages. This persistence within macrophages may be related to urease activity, mobilization of various stress responses and active evasion of the host defenses by cell surface sialylation. Conclusions/Significance The data presented here highlight the...

  12. Molecular characterisation of the early response in pigs to experimental infection with Actinobacillus pleuropneumoniae using cDNA microarrays

    Hedegaard, Jakob; Skovgaard, Kerstin; Mortensen, Shila;

    2007-01-01

    inflamed lung tissue, genes encoding immune activating proteins and other pro-inflammatory mediators of the innate immune response were found to be up-regulated. Genes encoding different acute phase reactants were found to be differentially expressed in the liver. Conclusion: The obtained results are......-inflamed lung tissue sampled from inoculated animals as well as in liver and tracheobronchial lymph node tissue sampled from three inoculated animals versus two non-inoculated animals. The lung samples were studied using a porcine cDNA microarray with 5375 unique PCR products while liver tissue and...... tracheobronchial lymph node tissue were hybridised to an expanded version of the porcine microarray with 26879 unique PCR products. Results: A total of 357 genes differed significantly in expression between infected and non-infected lung tissue, 713 genes differed in expression in liver tissue from infected versus...

  13. The Actinobacillus pleuropneumoniae HMW1C-Like Glycosyltransferase Mediates N-Linked Glycosylation of the Haemophilus influenzae HMW1 Adhesin

    Choi, Kyoung-Jae; Grass, Susan; Paek, Seonghee; St. Geme, Joseph W.; Yeo, Hye-Jeong

    2010-01-01

    The Haemophilus influenzae HMW1 adhesin is an important virulence exoprotein that is secreted via the two-partner secretion pathway and is glycosylated at multiple asparagine residues in consensus N-linked sequons. Unlike the heavily branched glycans found in eukaryotic N-linked glycoproteins, the modifying glycan structures in HMW1 are mono-hexoses or di-hexoses. Recent work demonstrated that the H. influenzae HMW1C protein is the glycosyltransferase responsible for transferring glucose and ...

  14. Identification of αLβ2, αMβ2, and αXβ2 integrins as receptors for Actinobacillus actinomycetemcomitans leukotoxin

    Reinholdt, Jesper; Poulsen, Knud; Kilian, Mogens; Vorup-Jensen, Thomas

    affect also interaction with LtxA has not been addressed. Activity of LtxA on test target cells has been evaluated by enumeration of dead cells stained with trypan blue. This is a tedious and error-prone method, particularly in titration assays. By the present study, we wanted to characterize in more...

  15. No overall relationship between average daily weight gain and the serological response to Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae in eight chronically infected Danish swine herds

    Andreasen, Margit; Mousing, Jan; Thomsen, Lars Krogsgård

    2001-01-01

    The association between the average daily weight gain (from approximately 4 to 20 weeks of age) and the serological responses to respiratory infections was examined in a longitudinal study including 825 pigs from eight chronically infected herds. Pigs were bled every 4th week (starting from......, early or late as compared to the median time (estimated across herds) of seroconversion for the particular pathogen. The variables "gender", "weight at an approximate age of 4 weeks" and "time" (defining the exact length of the follow-up period), were included as explanatory variables, and "litter......" and "herd" were included as explanatory random variables. The individual pig was the unit of concern. The variable defining time at seroconversion was not significantly associated with the average daily weight gain, when evaluating models across all eight herds. The apparent lack of effect could be because...

  16. Enriched Housing Reduces Disease Susceptibility to Co-Infection with Porcine Reproductive and Respiratory Virus (PRRSV) and Actinobacillus pleuropneumoniae (A. pleuropneumoniae) in Young Pigs.

    van Dixhoorn, Ingrid D E; Reimert, Inonge; Middelkoop, Jenny; Bolhuis, J Elizabeth; Wisselink, Henk J; Groot Koerkamp, Peter W G; Kemp, Bas; Stockhofe-Zurwieden, Norbert

    2016-01-01

    Until today, anti-microbial drugs have been the therapy of choice to combat bacterial diseases. Resistance against antibiotics is of growing concern in man and animals. Stress, caused by demanding environmental conditions, can reduce immune protection in the host, influencing the onset and outcome of infectious diseases. Therefore psychoneuro-immunological intervention may prove to be a successful approach to diminish the impact of diseases and antibiotics use. This study was designed to investigate the effect of social and environmental enrichment on the impact of disease, referred to as "disease susceptibility", in pigs using a co-infection model of PRRSV and A. pleuropneumoniae. Twenty-eight pigs were raised in four pens under barren conditions and twenty-eight other pigs were raised in four pens under enriched conditions. In the enriched pens a combination of established social and environmental enrichment factors were introduced. Two pens of the barren (BH) and two pens of the enriched housed (EH) pigs were infected with PRRSV followed by A. pleuropneumoniae, the other two pens in each housing treatment served as control groups. We tested if differences in disease susceptibility in terms of pathological and clinical outcome were related to the different housing regimes and if this was reflected in differences in behavioural and immunological states of the animals. Enriched housed pigs showed a faster clearance of viral PRRSV RNA in blood serum (p = 0.014) and histologically 2.8 fold less interstitial pneumonia signs in the lungs (p = 0.014). More barren housed than enriched housed pigs developed lesions in the lungs (OR = 19.2, p = 0.048) and the lesions in the barren housed pigs showed a higher total pathologic tissue damage score (pdisease susceptibility to co-infection of PRRSV and A. pleuropneumoniae in pigs. Enrichment positively influences behavioural state, immunological response and clinical outcome in pigs. PMID:27606818

  17. An Actinobacillus pleuropneumoniae PCR typing system based on the apx and omlA genes - evaluation of isolates from lungs and tonsils of pigs

    Gram, T.; Ahrens, Peter; Andreasen, Morten;

    2000-01-01

    . The PCR typing system was tested on 102 field strains of A. pleuropneumoniae isolated from lungs of diseased pigs. The serotyping results of the investigated field strains were in agreement with the apr and omlA gene patterns found in the reference strains of the bacteria, with the exception of the...... omlA gene of five strains of serotype 8. To examine the apx and omlA gene pattern of tonsil isolates, the PCR typing system was tested on a total of 280 A. pleuropneumoniae field strains isolated from tonsils of pigs. Agreement between serotyping and DNA typing was found in 96% of the isolates using...... the apx gene patterns and in 89% of the isolates using the omlA gene. The same serotype specific apx/omlA gene pattern was thus found in the majority of the tonsil isolates and in isolates from diseased lungs. Most of the differences in the omlA gene were found in 18 tonsil isolates of serotype 12...

  18. Validation of putative reference genes for qRT-PCR normalization in tissues and blood from pigs infected with Actinobacillus pleuropneumoniae

    Skovgaard, Kerstin; Mortensen, Shila; Poulsen, K.T.; Angen, Øystein; Heegaard, Peter M. H.

    The quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) is a sensitive and very efficient technique for quantification of gene expression. However, qRT-PCR relies on accurate normalization of gene expression data, as RNA recovery and cDNA synthesis efficiency might va...

  19. Hepatic gene expression changes in pigs experimentally infected with the lung pathogen Actinobacillus pleuropneumoniae as analysed with an innate immunity focused microarray

    Skovgaard, Kerstin; Mortensen, Shila; Boye, Mette;

    2010-01-01

    differentially expressed. A large group of these genes encoded proteins involved in the acute phase response, including serum amyloid A, C-reactive protein, fibrinogen, haptoglobin and tumor necrosis factor-a the expression of which were all found to be up-regulated and glutathione S-transferase, transthyretin...... initiating and orchestrating the innate immune response to A. pleuropneumoniae infection. Keywords: acute phase protein, hepatic transcriptional response, innate defence, gene expression, pig......, mannan-binding lectin A, surfactant protein D, and surfactant protein A1 were down-regulated in the liver of infected animals. Down-regulation of a1-acid glycoprotein during infection has not been described previously in any species. These results confirm that the liver plays an important role in...

  20. Estimation of sensitivity, specificity and predictive values of two serologic tests for the detection of antibodies against Actinobacillus pleuropneumoniae serotype 2 in the absence of a reference test (gold standard)

    Enøe, Claes; Andersen, Søren; Sørensen, Vibeke; Willeberg, Preben

    independence was assumed to models allowing for conditional dependence, given the true disease status. No strong evidence of conditional dependence in either test sensitivity or specificity was found. Assuming independence, maximum-likelihood estimates and 95% confidence intervals of the sensitivity and...

  1. Dicty_cDB: Contig-U05198-1 [Dicty_cDB

    Full Text Available e) Actinobacillus pleuropneumoniae ... 49 6e-08 CP001100_1460( CP001100 |pid:none) Chloroherpeton thalassi...bacterium freudenreichii ... 44 2e-08 CP000687_808( CP000687 |pid:none) Actinobacillus pleuropneumoni...000569_834( CP000569 |pid:none) Actinobacillus pleuropneumoniae ... 47 2e-07 CP000951_1090( CP000951 |pid:non...01213_1384( CP001213 |pid:none) Bifidobacterium animalis subsp.... 42 6e-07 CP000142_2488( CP000142 |pid:none) Pelobacter carbino...e) Chlorobaculum parvum NCIB 8327, ... 57 9e-07 AY625502_1( AY625502 |pid:none) Glossina morsitans morsitans clon

  2. Reduction of periodontal pathogens adhesion by antagonistic strains

    Van Hoogmoed, C. G.; Geertsema-Doornbusch, G. I.; Teughels, W.; Quirynen, M.; Busscher, H. J.; Van der Mei, H. C.

    2008-01-01

    Introduction: Periodontitis results from a shift in the subgingival micro. ora into a more pathogenic direction with Porphyromonas gingivalis, Prevotella intermedia, and Actinobacillus actinomycetemcomitans considered as periodontopathogens. In many cases, treatment procures only a temporary shift t

  3. 猪胸膜肺炎放线杆菌血清1型信号标签诱导突变体库的构建%CONSTRUCTION AND EVALUATION OF SIGNATURE-TAGGED MUTAGENESIS LIBRARIES OF ACTINOBACILLUS PLEUROP NEUMONIAE SEROTYPE

    赫明雷; 刘慧芳; 刘思国; 司薇; 王春来; 杨金国; 杜艳芬; 王聃

    2009-01-01

    本研究以自杀性质粒pUT携带含有信号标签的Mini-Tn5转座子对猪胸膜肺炎放线杆菌血清1型菌进行转座诱变,构建了带有12对特异性信号标签的Mini-Tn5转座子的pUT自杀质粒,转化到供体菌E.coliβ2155后,利用双亲本滤膜杂交法与受体猪胸膜肺炎放线杆菌血清1型菌(APP1)进行接合转移,构建并优化了接合转移体系.利用抗性和营养缺陷培养平板筛选得到接合突变体,通过抗性通用引物与12个特异标签引物和胸膜肺炎放线杆菌毒素IV(ApxIV)鉴定引物分别对这些突变体进行了PCR鉴定和测序验证.结果表明,经过加入标签的MinI-Tn5转座子可以通过接合转移的方式从供体菌E.COliβ2155中插入到APP1基因组当中,并成功构建了12个含有特异信号标签的重组质粒,获得了APP1的12个转座突变体库,经筛选鉴定后得到561个突变株.这为研究APP1的功能基因和筛选特定突变株提供了必要的基础.

  4. Generation of nalidixic acid-resistant strains and signature-tagged mutants of Actinobacillus pleuropneumoniae%胸膜肺炎放线杆菌萘啶酸抗性菌株的选育和信号标签突变株的构建

    商霖; 李薇; 李良军; 黎璐; 张四化; 李婷婷; 李耀坤; 刘磊; 郭志伟; 周锐; 陈焕春

    2008-01-01

    胸膜肺炎放线杆菌(APP)是重要的猪呼吸道病原菌,给世界养猪业造成严重的经济损失.信号标签突变(STM)技术是在宿主动物体内鉴定病原菌毒力因子的高通量方法.通过体外传代选育出APP血清1型和3型萘啶酸抗性菌株,再以萘啶酸抗性菌株为受体菌,以携带mini-Tn10的标签质粒(pLOF/TAG1-48)的E.coli CC118 λ pir或S17-1λpir为供体菌,在或不在E.coli DH5α(pRK2073)的辅助下,进行三亲本或两亲本接合,通过抗性筛选、PCR和Southern杂交鉴定转座突变株.结果表明:体外萘啶酸加压传代很容易选育出萘啶酸抗性APP菌株,该抗性的产生与DNA促旋酶A亚基基因gyrA的突变有关.在APP与E. coli接合实验中,两亲本接合比三亲本接合操作更简单,效率也较高;APP不同菌株在接合和转座效率上存在很大差异,血清1型菌株高于血清3型菌株,3型标准菌株高于地方分离株JL03-R.本研究为APP STM突变体库的构建与毒力基因的鉴定奠定了基础.

  5. Dicty_cDB: Contig-U15214-1 [Dicty_cDB

    Full Text Available e) Actinobacillus pleuropneumoniae... 107 4e-22 CP000927_3202( CP000927 |pid:none) Cau...lobacter sp. K31, complete g... 107 4e-22 CP000569_1501( CP000569 |pid:none) Actinobacillus pleuropneumoniae...000687_1497( CP000687 |pid:none) Actinobacillus pleuropneumoniae... 107 6e-22 (Q9...P001098_708( CP001098 |pid:none) Halothermothrix orenii H 168, co... 104 4e-21 (Q8RQM6) RecName: Full=Diaminopim... 83 9e-15 AE017283_1241( AE017283 |pid:none) Propionibacterium acnes KPA1712... 83 9e-15 (Q9X5M1) RecName: Full=Diaminopim

  6. Procedimiento para la detección, identificación y tipado de Haemophilus parasuis (Sistema DITPAR).

    Rodríguez Ferri, Elías Fernando; Navas Méndez, Jesús; Fuente Redondo, Víctor A. de la

    2001-01-01

    Se refiere un procedimiento de análisis molecular, por PCR-RFLP, que permite la detección, identificación y tipificación de Haemophilus parasuis, un microorganismo de interés en patología porcina, a partir de muestras clínicas y/o de aislamientos obtenidos en el laboratorio. El método se fundamenta en las peculiaridades de los genes tbp en esta bacteria cuando se compara con otras próximas, como Actinobacillus suis y Actinobacillus pleuropneumoniae. El procedimiento discrimina H. parasuis de ...

  7. Susceptibility of bacteria isolated from pigs to tiamulin and enrofloxacin metabolites

    Lykkeberg, Anne Kruse; Halling-Sørensen, Bent; Jensen, Lars Bogø

    -tiamulin (8 alpha-HTIA), and the ENR metabolites were: ciprofloxacin (CIP) and enrofloxacin N-oxide (ENR-N). Bacteria, all of porcine origin, we're selected as representatives of bacterial infections (Stap4ylococcus hyicus and Actinobacillus pleuropneumoniae), zoonotic bacteria (Campylobacter coli) and...

  8. Bacterial endocarditis due to eikenella corrodens: A case report

    Mahapatra A

    2003-01-01

    Full Text Available Of all the causes of bacterial endocarditis, HACEK group consisting of Haemophilus, Actinobacillus actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, and Kingella Kingae are rare causative agents. We report a case of bacterial endocarditis by E. corrodens, which is one of the members of the HACEK group.

  9. Knoflook remt App

    Mul, M.F.; Becker, P.M.; Peet-Schwering, van der C.M.C.; Wikselaar, van P.G.; Wisselink, H.J.; Stockhofe, N.

    2011-01-01

    De Animal Sciences Group van Wageningen UR heeft in opdracht van biologische varkenshouders onderzocht of het mogelijk is om in plaats van antibiotica, knoflook te gebruiken voor de bestrijding van longontsteking door de bacterie Actinobacillus pleuropneumoniae (App). Uit de resultaten blijkt dat Al

  10. Bacteria interfere with A-actinomycetemcomitans colonization

    Teughels, Wim; Haake, S. Kinder; Sliepen, Isabelle; Pauwels, Martine; Van Eldere, Johan; Cassiman, Jean-Jacques; Quirynen, Marc

    2007-01-01

    It is known that beneficial bacteria can suppress the emergence of pathogenic bacteria, particularly in the gastrointestinal tract. This study examined the potential for a similar suppression of Aggregatibacter (formerly Actinobacillus) actinomycetemcomitans colonization of epithelial cells, due to its potential relevance in periodontal diseases. Seven presumed beneficial bacteria were examined for their ability to interfere, exclude, or displace A. actinomycetemcomitans from epithelial cells...

  11. Expression of coding (mRNA) and non-coding (microRNA) RNA in lung tissue and blood isolated from pigs suffering from bacterial pleuropneumonia

    Skovgaard, Kerstin; Schou, Kirstine Klitgaard; Wendt, Karin Tarp;

    2010-01-01

    infected with Actinobacillus pleuropneumoniae (AP). Expression differences of mRNA and microRNA were quantified at different time points (6h, 12h, 24h, 48h PI) using reverse transcription quantitative real-time PCR (Rotor-Gene and Fluidigm). Expression profiles of miRNA in blood of seven animals were...

  12. The concentration of apolipoprotein A-I decreases during experimentally induced acute-phase processes in pigs

    Carpintero, R.; Pineiro, M.; Andres, M.;

    2005-01-01

    In this work, apolipoprotein A-I (ApoA-I) was purified from pig sera. The responses of this protein after sterile inflammation and in animals infected with Actinobacillus pleuropneumoniae or Streptococcus suis were investigated. Decreases in the concentrations of ApoA-I, two to five times lower...

  13. 21 CFR 522.1662a - Oxytetracycline hydrochloride injection.

    2010-04-01

    ... Federal Register citations affecting § 522.1662a, see the List of CFR Sections Affected, which appears in... tongue (Actinobacillus lignieresi), leptospirosis (Leptospira pomona), and wound infections; acute... Leptospira pomona, and wound infections and acute metritis caused by Staphylococcus spp. and...

  14. Infectious and rearing-system related risk factors for chronic pleuritis in slaughter pigs

    Enøe, Claes; Mousing, Jan; Schirmer, Anna Luise; Willeberg, Preben

    2002-01-01

    of the pig, the weight of the carcass, and the herd of origin were also recorded. Individual blood samples were examined for seropositivity for Actinobacillus pleuropneumoniae (AP) serotypes 2, 6, 7, 12, Haemophilus parasuis, Mycoplasma hyopneumoniae (MYC) and swine influenza (SI). Herd...

  15. Changes in the incidence of periodontal pathogens during long-term monitoring and after application of antibacterial drugs

    Janatová, T.; Najmanová, Lucie; Neubauerová, Lenka; Kyselková, Martina; Novotná, Gabriela; Spížek, Jaroslav; Janata, Jiří; Dušková, J.

    2009-01-01

    Roč. 54, č. 5 (2009), s. 429-435. ISSN 0015-5632 R&D Projects: GA MŠk 1M06011; GA MZd NR9119 Institutional research plan: CEZ:AV0Z50200510 Keywords : DUAL-SPECIES BIOFILMS * ACTINOBACILLUS-ACTINOMYCETEMCOMITANS * PORPHYROMONAS-GINGIVALIS Subject RIV: EE - Microbiology, Virology Impact factor: 0.978, year: 2009

  16. INVESTIGATION OF PERIODONTITIS PATIENTS AND COMPARISON WITH CONTROL GROUP

    M.H. Satan

    1998-09-01

    Full Text Available The bacterial population found in the gingival sulcus region in periodontitis consists of gram negative microorganisms; most of them are obligate anaerobes and capnophil, including Porphyromonas, Prevotella, Fusobaclenuin, Welinella, Actinobacillus actinomycetem comitans, Eiknella and Capnocytohaga. This study has been carried out to investigate anaerobic and capnophilic bacteria from 206 specimens of patients with periodontitis and 145 specimens of control group. Different bacteria isolated from total specimens were obtained as follows: Actinobacillus actinomycetem comitans (patients group 36.9%, control group 4.1 %, Capnocytophaga (47.9%, Eikenella corodens (34.5% , 10.3%, Porphyryromonas gingivaIis (35% , 11%, Prevotella intermedius25.7%, 6.7% and Prevotella melaninogenicus (13.6% , 3.4%.

  17. Poly-N-Acetylglucosamine Matrix Polysaccharide Impedes Fluid Convection and Transport of the Cationic Surfactant Cetylpyridinium Chloride through Bacterial Biofilms▿

    Ganeshnarayan, Krishnaraj; Shah, Suhagi M.; Libera, Matthew R.; Santostefano, Anthony; Kaplan, Jeffrey B.

    2008-01-01

    Biofilms are composed of bacterial cells encased in a self-synthesized, extracellular polymeric matrix. Poly-β(1,6)-N-acetyl-d-glucosamine (PNAG) is a major biofilm matrix component in phylogenetically diverse bacteria. In this study we investigated the physical and chemical properties of the PNAG matrix in biofilms produced in vitro by the gram-negative porcine respiratory pathogen Actinobacillus pleuropneumoniae and the gram-positive device-associated pathogen Staphylococcus epidermidis. Th...

  18. Enzymatic Detachment of Staphylococcus epidermidis Biofilms

    Kaplan, Jeffrey B.; Ragunath, Chandran; Velliyagounder, Kabilan; Fine, Daniel H.; Ramasubbu, Narayanan

    2004-01-01

    The gram-positive bacterium Staphylococcus epidermidis is the most common cause of infections associated with catheters and other indwelling medical devices. S. epidermidis produces an extracellular slime that enables it to form adherent biofilms on plastic surfaces. We found that a biofilm-releasing enzyme produced by the gram-negative periodontal pathogen Actinobacillus actinomycetemcomitans rapidly and efficiently removed S. epidermidis biofilms from plastic surfaces. The enzyme worked by ...

  19. Analysis of the presence of pathogens which predict the risk of disease at peri-implant sites through polymerase chain reaction (PCR) Análise por reação em cadeia da polimerase (PCR) da presença de patógenos preditores de risco em sítios periimplantares

    Joely Ângela de Oliveira Leitão; José Luiz De Lorenzo; Mario Julio Avila-Campos; Wilson Roberto Sendyk

    2005-01-01

    The presence of DNA of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Prevotella intermedia in the peri-implant sulcus samples of 19 partially edentulous patients was analyzed by polymerase chain reaction (PCR) and related to the depth of the peri-implant sulcus, bleeding on probing, and probable risk of disease. Ten of those patients presented a history of periodontal disease and nine of those did not. The DNA amplification of these pathogens was observed in seven sample...

  20. Cardiobacterium hominis-induced acute dacryocystitis and lacrimal abscess

    Guru Prasad Manderwad

    2014-01-01

    Full Text Available Cardiobacterium hominis is a member of the HACEK (Haemophilus sp., Actinobacillus actinomycetemcomitans, C. hominis, Eikenella corrodens, and Kingella kingae group commonly associated with endocarditits and is normally present in the respiratory tract. We describe the first case of acute dacryocystitis with lacrimal abscess caused by C. hominis along with a brief review of the literature. The patient responded to oral and topical ciprofloxacin after incision and drainage and awaits dacryocystorhinostomy.

  1. Combined orthodontic and periodontic treatment in a child with Papillon Lefèvre syndrome

    AlSarheed, Maha A.; Al-Sehaibany, Fares S.

    2015-01-01

    A 9-year-old girl with Papillon-Lefèvre syndrome (PLS) was treated orthodontically 24 months after the start of mechanical and antibiotic therapy in adjunct with periodontal treatment every 6 weeks. After achieving stable periodontal conditions, orthodontic treatment was commenced to correct the teeth position, facial profile, and maxillary protraction. Following the combination therapy and a failure to detect Actinobacillus actinomycetemcomitans from any site in the oral cavity, orthodontic ...

  2. Cardiobacterium hominis endocarditis: two cases and a review of the literature

    Malani, A. N.; Aronoff, D. M.; Bradley, S. F.; Kauffman, C A

    2006-01-01

    Cardiobacterium hominis, a member of the HACEK group (Haemophilus parainfluenzae, Haemophilus aphrophilus, and Haemophilus paraphrophilus, Actinobacillus actinomycetemcomitans, C. hominis, Eikenella corrodens, and Kingella species), is a rare cause of endocarditis. There are 61 reported cases of C. hominis infective endocarditis in the English-language literature, 15 of which involved prosthetic valve endocarditis. There is one reported case of C. hominis after upper endoscopy and none report...

  3. Attachment of oral bacteria to a basement-membrane-like matrix and to purified matrix proteins.

    Winkler, J R; S. R. John; Kramer, R H; Hoover, C.I.; Murray, P A

    1987-01-01

    The purpose of this study was to investigate the adherence of oral bacteria to an in vitro basement-membrane-like matrix and to selected individual macromolecular constituents of this matrix. Radiolabeled bacteria were incubated with basement-membrane-like matrices isolated from PF HR-9 cells. Bacteroides gingivalis 33277, Fusobacterium nucleatum FN-2, and Actinobacillus actinomycetemcomitans GA3(A) bound to the matrix in the range of 44 to 70%, considerably higher than the ranges of A. actin...

  4. Bases farmacomicrobiológicas del tratamiento antibiótico de las enfermedades periodontales y periimplatarias Farmacobiological concepts in the antibiotic treatment of the periodontal diseases

    Liñares, J.; J.E. Martín-Herrero

    2003-01-01

    La enfermedad periodontal debe considerarse un proceso infeccioso bacteriano crónico. En su etiología, no hay una única especie bacteriana implicada, sino que podríamos considerarla como una infección polimicrobiana en la que estarían implicados diversos microorganismos. Las bacterias que se han asociado más directamente con la enfermedad periodontal son Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Bacteroides forsythus y Treponema denticola. Los pará...

  5. Prevalence of Periodontal Pathogens in Dental Plaque of Children

    Gafan, Gavin P.; Lucas, Victoria S.; Roberts, Graham J.; Petrie, Aviva; Wilson, Michael; David A. Spratt

    2004-01-01

    Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, and Tannerella forsythensis have been implicated as the main etiological agents of periodontal disease. The purpose of this work was to estimate the prevalence of these organisms in plaque from children without gingivitis (group 1; n = 65) and from those with gingivitis (group 2; n = 53). Extracted DNA from plaque was subjected to two rounds of PCR targeting the 16S rRNA gene using both universal primers and species-specific prim...

  6. Valorización biotecnológica de subproductos/residuos industriales: producción de ácido succínico y ácido láctico.

    Ramos Andrés, Marta

    2015-01-01

    Se ha estudiado el proceso biotecnológico de obtención de ácidos orgánicos (ácido succínico y ácido láctico) por Actinobacillus succinogenes. Ambos ácidos tienen aplicaciones en alimentación, farmacia e industria química y de polímeros. Su obtención mediante un bioproceso presenta múltiples ventajas como la reducción de las necesidades energéticas y de emisiones de gases de efecto invernadero, así como el aprovechamiento de subproductos/residuos industriales. Se han estudio ...

  7. Investigations of representation of certain bacteria strains in lungs of pigs with pneumonia

    Žutić Milenko

    2009-01-01

    Full Text Available The objective of the investigations described in this paper was to carry out the identification and to establish the incidence of certain strains of bacteria that take part in the etiopathogenesis of pig infections. The investigations covered a total of 237 pathoanatomically altered lungs of expired pigs. Sampling was done during visits to pig farms, most often in situations when it had been necessary to resolve occurring respiratory infections. The samples were examined in a laboratory for the presence of bacterial causes using standard and commercial methods of microbiological diagnostics. For this purpose, the samples were sown on corresponding nutritive bases (blood agar, MacConkey agar, nutritive agar, BHI agar, Baird Parker agar. For the primary isolation of Actinobacillus pleuropneumoniae and Haemophilus parasuis, agar with 5-10% sheep's blood was used and the culture of the strain Staphylococcus aureus which serves as a source of V factor, and for subcultivation of these causes, chocolate agar was used with PolyVitex. In the isolated bacteria, following investigations of morphological and culture characteristics, biochemical identification was performed using the commercial tests BBL Crystal GP ID Kit, E/N ID Kit, Api 20 Strep and Slidex Staph Plus. From the total of 237 examined lung samples, 13 bacteria strains were isolated from 193 samples (81.43%. Among breeding pigs, 112 lung samples were examined and the presence of bacteria was established in 92 (82.14%, while the presence of bacteria was established in 101 samples (80.8% of 125 examined lung samples from fattening pigs. Two bacteria strains were dominant among the spectrum of lung microorganisms: Pasteurella multocida (32.64% and Actinobacillus pleuropneumoniae (29.02%, or, these two bacteria strains in total accounted for 61.66% of all strains isolated in pure culture. The participation of the other 11 bacteria strains ranged from 0.52-9.84%. Observed according to production

  8. Recombinant microorganisms for increased production of organic acids

    Yi, Jian; Kleff, Susanne; Guettler, Michael V

    2013-04-30

    Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

  9. Veterinary research, monitoring and advisory services in connection with the establishment and operation of a communal biomass conversion plant. Partial project 3 (VET-BIO-3). Veterinaer forskning, overvaagning og raadgivning i forbindelse med etablering og drift af biogasfaellesanlaeg. Delprojekt 3 (VET-BIO-3); Pilotprojekt vedroerende etablering af sygdomsovervaagning i forbindelse med biogasfaellesanlaeg

    Willadsen, C.M.

    1991-06-15

    The health of domestic animals which contribute to thermophilic biomass conversion plants has been monitored during a period of 15-18 months. The aim was to evaluate the possible risk of infection by animal diseases through liquid manures which are converted in the plants. Data from 39 stocks has been collected and evaluated for the surveilliance of the general health conditions and the occurrence of specific germs, for cattle Salmonella Dublin, and for swine Actinobacillus pleuropneumiae (serotype 2) and Treponema hyodysenteriae. (CLS) 24 refs.

  10. AcEST: DK947761 [AcEST

    Full Text Available SE Testican-2 OS=Mus musculus GN=Spock2 PE=2 ... 32 1.7 sp|A6VQ42|RNFC_ACTSZ Electron transport complex prot...|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 SV=1 Length = 423 Score = 32.3 bits (72), Expect = 1....rt complex protein rnfC OS=Actinobacillus succinogenes (strain ...PKHD-type hydroxylase SYNPCC7002_A2658 OS=Synechococcus sp. (strain ATCC 27264 / ...2 Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0016_O16. 5' end sequence. Accession DK94776

  11. Dicty_cDB: Contig-U15683-1 [Dicty_cDB

    Full Text Available e) Yarrowia lipolytica strain CLIB1... 108 4e-22 AM494964_221( AM494964 |pid:none) Leishmania braziliensis chromos...e) Ralstonia pickettii 12J chromoso... 128 5e-28 CP001359_3546( CP001359 |pid:none) An...mic... 98 2e-19 AM502247_265( AM502247 |pid:none) Leishmania infantum chromos...e) Lactobacillus fermentum IFO 3956... 91 1e-16 AM494966_227( AM494966 |pid:none) Leishmania braziliensis chromos...o... 91 1e-16 CP000687_72( CP000687 |pid:none) Actinobacillus pleuropneumoni

  12. Dicty_cDB: Contig-U11369-1 [Dicty_cDB

    Full Text Available 664262_1( FJ664262 |pid:none) Annona cherimola disproportionatin... 279 1e-73 AY081315_1( AY081315 |pid:none) Arabidopsi...transferase; EC=2... 55 3e-06 CP000950_161( CP000950 |pid:none) Yersinia pseudotuberculosis YPII... 55 4...e) Escherichia coli IAI39 chromoso... 55 4e-06 FM178380_39( FM178380 |pid:none) Aliivibrio salmoni...a, complet... 53 2e-05 CP001069_163( CP001069 |pid:none) Ralstonia pickettii 12J chromos...s KD131 ch... 43 0.017 CP000687_1202( CP000687 |pid:none) Actinobacillus pleuropneumoni

  13. Epidimitis ovina: Estudios bacteriológico y serológico

    Graciela Méndez Nárez; Efrén Díaz Aparicio; José Francisco Morales Álvarez; Francisco Aguilar Romero; Francisco Suárez Güemes

    1999-01-01

    La brucelosis en ovinos puede ser causada por Brucella ovis y por brucelas lisas, en ambos casos puede presentarse epididimitis u orquitis, las cuales también se asocian con otros agentes infecciosos como Actinobacillus seminis e Histophilus ovis. El objetivo de este trabajo fue aislar e identificar los agentes bacterianos involucrados en la epididimitis ovina y establecer la relación en el diagnóstico serológico y bacteriológico de brucelas lisas, de B. ovis y A. seminis con la p...

  14. Dicty_cDB: Contig-U09340-1 [Dicty_cDB

    Full Text Available um geno... 36 0.68 2 ( DQ198149 ) Plasmodium falciparum merozoite-associated trypto... 34 0.72 4 ( FH170...(s) est1= SSD301F ,1,329 est2= SSD301Z ,330,593 Translated Amino Acid sequence klrfsf*cycyccsihynniac...e chromosome V lambda clones... 34 0.23 2 ( X65554 ) S.cerevisiae ICL1 gene for isoci...oda cDNA cl... 46 1.4 1 ( CP000746 ) Actinobacillus succinogenes 130Z, complete g...KIKIISKMPPICNSIENENNSFELTTTISFLKKSKLFKKEINVNEIVSINIS QILGEYNSFLINNTIEDLESAIAQNIHN*s Translated Amino Aci

  15. Interferência do tratamento preventivo com antimicrobianos sobre a colonização de agentes da microbiota do trato respiratório de leitões

    Lara, Anne Caroline de

    2012-01-01

    Resumo: Os agentes Streptococcus suis, Haemophilus parasuis e Actinobacillus pleuropneumoniae são responsáveis por grandes prejuízos na suinocultura tecnificada. Estes agentes podem causar meningite estreptocócica, doença de Glasser ou pneumonias, respectivamente. Essas doenças são consideradas multifatoriais, pois somente a presença dos agentes na granja não é suficiente para desencadear a doença. Portanto, a presença de fatores de risco associado à cepas potencialmente patogênicas represent...

  16. Identification of Dominant Immunogenic Bacteria and Bacterial Proteins in Periodontitis

    Agerbæk, Mette Rylev; Haubek, Dorte; Birkelund, Svend;

    Marginal periodontitis is considered an infectious disease that triggers host inflammatory responses resulting in destruction of the periodontium. A complex biofilm of bacteria is associated with periodontitis. Some species have been identified as putative pathogens such as Porphyromonas gingivalis...... (P.g) and Actinobacillus actinomycetemcomitans (A.a), but the identity of dominate immunogens of these bacteria is poorly elucidated. The aim of the study was to identify dominant immunogenic proteins of P.g and A.a in patients suffering from chronic and aggressive periodontitis by proteomic analysis...... will be able to identify immunodominant proteins and potentially important virulence factors of putative periodontal pathogens....

  17. Dicty_cDB: Contig-U09733-1 [Dicty_cDB

    Full Text Available AE000516 |pid:none) Mycobacterium tuberculosis CDC1... 100 8e-20 AM408590_1682( AM408590 |pid:none) Mycobacterium bovis BCG Pasteur...ELNNPFVQIERFAEQAKAKAAGDDESMLIDKVFT TSLEYGLPPTGGFGLGIDRFAMLMSDTYNIKEVILFPAMKPE*vskpvttttaaptttea pksn*vqlfnyf...SDTYNIKEVILFPAMKPE*vskpvttttaaptttea pksn*vqlfnyfknfkntliqiynknnykinqff Frame B: ...81 |pid:none) Thauera sp. MZ1T, complete geno... 133 1e-29 CP001091_868( CP001091 |pid:none) Actinobacillus pleuropneu...trophilus V24St... 113 1e-23 CP001472_268( CP001472 |pid:none) Acidobacterium capsulatum ATCC 5... 113 1e-23

  18. 伴放线凝聚杆菌感染性心内膜炎一例分析

    刘薇; 云峰; 陈龙; 杨洪波; 王拓; 王晓玲; 姚瑶; 徐珊; 王玉彬; 赖惠英; 陈雪; 陶凤蓉; 艾效曼; 陈东科; 许宏涛; 胡云建

    2014-01-01

    感染性心内膜炎(infective endocarditis,IE)为细菌、真菌和其他微生物(如病毒、立克次体、衣原体、螺旋体等)直接感染而产生心瓣膜或心室壁内膜的炎症。嗜血杆菌属(Haemophilus species)、放线杆菌属(Actinobacillus actinomycetemcomitans)、心杆菌属(Cardiobacterium hominis)、艾肯菌属(Eikenella corrodens)、

  19. Anti-microbial Activity of Tulsi {Ocimum Sanctum (Linn.)} Extract on a Periodontal Pathogen in Human Dental Plaque: An Invitro Study

    Devaraj, C.G.; Agarwal, Payal

    2016-01-01

    Introduction Tulsi is a popular healing herb in Ayurvedic medicine. It is widely used in the treatment of several systemic diseases because of its anti-microbial property. However, studies documenting the effect of Tulsi on oral disease causing organisms are rare. Hence, an attempt was made to determine the effect of Tulsi on a periodontal microorganism in human dental plaque. Aim To determine if Ocimum sanctum (Linn.) has an anti-microbial activity (Minimum Inhibitory Concentration and zone of inhibition) against Actinobacillus actinomycetemcomitans in human dental plaque and to compare the antimicrobial activity of Ocimum sanctum(Linn.) extract with 0.2% chlorhexidine as the positive control and dimethyl sulfoxide as the negative control. Materials and Methods A lab based invitro experimental study design was adopted. Ethanolic extract of Ocimum sanctum (Linn.) was prepared by the cold extraction method. The extract was diluted with an inert solvent, dimethyl sulfoxide, to obtain ten different concentrations (1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%) of extract. Plaque sample was collected from 05 subjects diagnosed with periodontal disease. Isolation of Actinobacillus actinomycetemcomitans from plaque samples was done using Tryptic Soy Serum Bacitracin Vancomycin agar (TSBV) medium. Identification of Actinobacillus actinomycetemcomitans was done based on cultural, microscopic, biochemical characterization and multiple drug resistance patterns. Anti-microbial activity of Ocimum sanctum (Linn.) extract was tested by agar well-diffusion method against 0.2% chlorhexidine as a positive control and dimethyl sulfoxide as a negative control. The zone of inhibition was measured in millimeters using Vernier callipers. Results At the 6% w/v concentration of Ocimum sanctum (Linn.) extract, a zone of inhibition of 22 mm was obtained. This was the widest zone of inhibition observed among all the 10 different concentrations tested. The zone of inhibition for positive control

  20. Antimicrobial activity of magnolol and honokiol against periodontopathic microorganisms.

    Chang, B; Lee, Y; Ku, Y; Bae, K; Chung, C

    1998-05-01

    Magnolol (1) and honokiol (2), main compounds from the stem bark of Magnolia obovata Thunb., were evaluated for an antimicrobial activity against periodontopathic microorganisms, Porphyromonas gingivalis, Prevotella gingivalis, Actinobacillus actinomycetemcomitans, Capnocytophaga gingivalis, and Veillonella disper, and a cytotoxicity against human gingival fibroblasts and epithelial cells. Our results indicate that magnolol and honokiol, although less potent than chlorhexidine, show a significant antimicrobial activity against these microorganisms, and a relatively low cytotoxic effect on human gingival cells. Thus, it is suggested that magnolol and honokiol may have a potential therapeutic use as a safe oral antiseptic for the prevention and the treatment of periodontal disease. PMID:9619121

  1. Dicty_cDB: Contig-U12795-1 [Dicty_cDB

    Full Text Available M_Contig/Contig-U12795-1Q.Seq.d (799 letters) Database: ddbj_A 92,845,959 sequences; 95,242,211,685 total letters Searchi...Actinobacillus pleuropneumoniae ... 39 0.15 CP000264_969( CP000264 |pid:none) Jannaschia sp. CCS1, complete ...ti ge... 44 8.0 1 ( CN187962 ) UCRCS05_0005E19_f Washington Navel Orange Stored ...... 44 8.0 1 ( CN186686 ) UCRCS05_0003E16_f Washington Navel Orange Stored ... 44 8.0 1 ( CN186684 ) UCRCS05_0003E15_f Washington Nave...l Orange Stored ... 44 8.0 1 ( CN185617 ) UCRCS05_0001J21_f Washington Navel Orange Sto

  2. High-throughput Gene Expression Analysis In Pigs As Model For Respiratory Infections

    Skovgaard, Kerstin; Brogaard, Louise; Schou, Kirstine Klitgaard;

    perform highly controlled experimental infections and to study changes of symptoms, viral titer, and expression of microRNAs/mRNAs as the influenza infection progresses in time, generating information that would be difficult to obtain from human patients. The Gram-negative bacterium Actinobacillus......Influenza A virus infections have great impact on human health and welfare and significant resources are linked to influenza epidemics due to excess hospitalizations and lost productivity. Up to 15% of the human population is affected when Influenza spreads around the world in seasonal epidemics...... (WHO). Animal models are essential in understanding the mechanisms involved in human infectious disease and for the development of effective prevention and treatment strategies. It is increasingly realized that large animal models like the pig are exceptionally human like and serve as an excellent...

  3. Obtención de ácido láctico. Fermentación en fase dual mediante Actinobacilus Succinogenes

    Ayala Varela, Flavio Celín

    2015-01-01

    Se presentan los resultados obtenidos para la obtención de ácido láctico partiendo de glucosa como sustrato, utilizando el microorganismo Actinobacillus succinogenes, favoreciendo la ruta menos común hacia el piruvato con las condiciones adecuadas: Medio de crecimiento; 53286 Brain Heart Broth (SIGMA-ALDRICH), medio de fermentación; 60 g glucosa, 30 g extracto de levadura, 2 g urea, 2 g MgCl2·6H2O, 1.99 g CaCl2. 2H2O, 0.11 g MnCl2. 4H2O, 4.4 g Na2HPO4, 4,06 g NaH2PO4. 2H2O (composición por li...

  4. Thermochemical pretreatments for enhancing succinic acid production from industrial hemp (Cannabis sativa L.).

    Gunnarsson, Ingólfur B; Kuglarz, Mariusz; Karakashev, Dimitar; Angelidaki, Irini

    2015-04-01

    The aim of this study was to develop an efficient thermochemical method for treatment of industrial hemp biomass, in order to increase its bioconversion to succinic acid. Industrial hemp was subjected to various thermochemical pretreatments using 0-3% H2SO4, NaOH or H2O2 at 121-180°C prior to enzymatic hydrolysis. The influence of the different pretreatments on hydrolysis and succinic acid production by Actinobacillus succinogenes 130Z was investigated in batch mode, using anaerobic bottles and bioreactors. Enzymatic hydrolysis and fermentation of hemp material pretreated with 3% H2O2 resulted in the highest overall sugar yield (73.5%), maximum succinic acid titer (21.9 g L(-1)), as well as the highest succinic acid yield (83%). Results obtained clearly demonstrated the impact of different pretreatments on the bioconversion efficiency of industrial hemp into succinic acid. PMID:25682224

  5. Dicty_cDB: Contig-U06293-1 [Dicty_cDB

    Full Text Available lete... 50 2e-05 AE008692_592( AE008692 |pid:none) Zymomonas mobilis subsp. mobilis... 50 3e-05 AP006725_402...68J8) RecName: Full=UPF0451 protein C17orf61 homolog; Flags: ... 57 2e-07 CP000117_3881( CP000117 |pid:none) Anabaena variabili...) Frame A: rtlvenwwn*rl*cyyfrclwwtwikeksy*cstsrylencttipslsficlipcsilnt tkycwsnvfnwnfii*wiii*fityk*knrcfsnwwilvygrlgnfrndir*iiifkkli...bacter usitatus Ellin6076, ... 54 1e-06 CP000569_678( CP000569 |pid:none) Actinobacillus pleuropneumo... |pid:none) Escherichia coli APEC O1, compl... 46 4e-04 AM942759_2277( AM942759 |pid:none) Proteus mirabil

  6. Laminaria digitata as a potential carbon source for succinic acid and bioenergy production in a biorefinery perspective

    Alvarado-Morales, Merlin; Gunnarsson, Ingólfur Bragi; Fotidis, Ioannis;

    2015-01-01

    corresponded to 298 and 285 NmL CH4 g− 1 VSadded, respectively. PHSR could potentially be used for: dietary food additive, fish feed, bioenergy production and added value products. This study opens possibility to conceive different biorefinery scenarios in which the efficient use of the macroalgal biomass......A novel biorefinery concept utilizing macroalgae Laminaria digitata to produce succinic acid, and direct the process residues for feed and energy production, is investigated in the present study. Enzymatic hydrolysis was performed at high solid loading (25% w v− 1) resulting in solubilization of...... the carbohydrates to soluble sugars, which accumulated in the liquid hydrolysate. The overall sugar recovery in the macroalgae hydrolysate was 78.23%. Actinobacillus succinogenes 130Z was able to ferment macroalgae hydrolysate to succinic acid with a yield of 86.49% (g g− 1 of total sugars) and an...

  7. AcEST: DK950383 [AcEST

    Full Text Available ASTTDS 194 >sp|A6VQ42|RNFC_ACTSZ Electron transport complex protein rnfC OS=Actinobacillus succi...bits) Value sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 ... 32 1.7 sp|A6VQ42|RNFC_ACTSZ Electron transpo...sapiens GN... 30 8.5 >sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 SV=1 Length = 423 Scor...AAV 643 >sp|B1XLW1|Y2658_SYNP2 PKHD-type hydroxylase SYNPCC7002_A2658 OS=Synechococc...4 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0008_I05. 5' end sequence. Accession DK95038

  8. AcEST: DK961319 [AcEST

    Full Text Available Value sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 ... 32 2.3 ...kinase OS=Corynebacterium glutamic... 30 8.7 >sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=...ex protein rnfC OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=rnfC PE=3 SV=1 Length = 703 Sco...KQLAVRCEGPCPCP 183 >sp|B1XLW1|Y2658_SYNP2 PKHD-type hydroxylase SYNPCC7002_A2658 OS=Synechococc...1 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0009_M16. 5' end sequence. Accession DK96131

  9. AcEST: DK953954 [AcEST

    Full Text Available >sp|A6VQ42|RNFC_ACTSZ Electron transport complex protein rnfC OS=Actinobacillus succi... sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 ... 32 1.6 sp|A6VQ42|RNFC_ACTSZ Electron transpo..... 30 7.8 >sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 SV=1 Length = 423 Score = 32.3 bi...|B1XLW1|Y2658_SYNP2 PKHD-type hydroxylase SYNPCC7002_A2658 OS=Synechococcus sp. (...0 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0019_C10. 5' end sequence. Accession DK95395

  10. AcEST: DK947980 [AcEST

    Full Text Available ....done Score E Sequences producing significant alignments: (bits) Value sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spoc...RNFC_ACTSZ Electron transport complex protein rnfC OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z)...can-2 OS=Mus musculus GN=Spock2 PE=2 SV=1 Length = 423 Score = 32.3 bits (72), Expect = 2.2 Identities = 16/...8_SYNP2 PKHD-type hydroxylase SYNPCC7002_A2658 OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN...5 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0002_B10. 5' end sequence. Accession DK94798

  11. AcEST: DK953389 [AcEST

    Full Text Available >sp|A6VQ42|RNFC_ACTSZ Electron transport complex protein rnfC OS=Actinobacillus succi...e sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 ... 32 1.6 sp|A6VQ42|RNFC_ACTSZ Electron transpo...... 30 7.8 >sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 SV=1 Length = 423 Score = 32.3 b...p|B1XLW1|Y2658_SYNP2 PKHD-type hydroxylase SYNPCC7002_A2658 OS=Synechococcus sp. ...1 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0017_K07. 5' end sequence. Accession DK95338

  12. AcEST: DK943994 [AcEST

    Full Text Available CLSSKQLAVRCEGPCPCPTEQSTASTTDS 194 >sp|A6VQ42|RNFC_ACTSZ Electron transport complex protein rnfC OS=Actinobacillus succi...ng significant alignments: (bits) Value sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 ......ens GN... 30 9.4 >sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 SV=1 Length = 423 Score = ...-type hydroxylase SYNPCC7002_A2658 OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN=SYNPCC7002_...1 Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0004_K07. 5' end sequence. Accession DK94399

  13. AcEST: DK953205 [AcEST

    Full Text Available icant alignments: (bits) Value sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 ... 32 1.8 sp...d box protein J3 OS=Homo sapiens GN... 30 8.7 >sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spoc...lex protein rnfC OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=rnf...GP PCP Sbjct: 160 KLEQQACLSSKQLAVRCEGPCPCP 183 >sp|B1XLW1|Y2658_SYNP2 PKHD-type hydroxylase SYNPCC7002_A2658 OS=Synechococc...0 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0017_C14. 5' end sequence. Accession DK95320

  14. AcEST: DK951024 [AcEST

    Full Text Available QLAVRCEGPCPCPTEQSTASTTDS 194 >sp|A6VQ42|RNFC_ACTSZ Electron transport complex protein rnfC OS=Actinobacillus succi...can-2 OS=Mus musculus GN=Spock2 PE=2 ... 32 2.3 sp|A6VQ42|RNFC_ACTSZ Electron transpo...c... 30 8.7 >sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 SV=1 Length = 423 Score = 32.3 ...58_SYNP2 PKHD-type hydroxylase SYNPCC7002_A2658 OS=Synechococcus sp. (strain ATCC...1 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0010_E15. 5' end sequence. Accession DK95102

  15. AcEST: DK954517 [AcEST

    Full Text Available EQSTASTTDS 194 >sp|A6VQ42|RNFC_ACTSZ Electron transport complex protein rnfC OS=Actinobacillus succinogenes ...2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 ... 32 2.6 sp|A6VQ42|RNFC_ACTSZ Electron transport complex...p|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 SV=1 Length = 423 Score = 32.3 bits (72), Exp...DPKKAAV 643 >sp|B1XLW1|Y2658_SYNP2 PKHD-type hydroxylase SYNPCC7002_A2658 OS=Synechococcus sp. (strain ATCC ...1 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0020_K10. 5' end sequence. Accession DK95451

  16. AcEST: DK944835 [AcEST

    Full Text Available 94 >sp|A6VQ42|RNFC_ACTSZ Electron transport complex protein rnfC OS=Actinobacillus succi...lue sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 ... 32 1.8 sp|A6VQ42|RNFC_ACTSZ Electron transpo... (... 30 8.9 >sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 SV=1 Length = 423 Score = 32.3...658_SYNP2 PKHD-type hydroxylase SYNPCC7002_A2658 OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) ...5 Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0007_F11. 5' end sequence. Accession DK94483

  17. AcEST: DK954687 [AcEST

    Full Text Available PTEQSTASTTDS 194 >sp|A6VQ42|RNFC_ACTSZ Electron transport complex protein rnfC OS=Actinobacillus succinogene...R58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 ... 32 2.1 sp|A6VQ42|RN...>sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 SV=1 Length = 423 Score = 32.3 bits (72), E...PDPKKAAV 643 >sp|B1XLW1|Y2658_SYNP2 PKHD-type hydroxylase SYNPCC7002_A2658 OS=Synechococcus sp. (strain ATCC...0 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0021_B17. 5' end sequence. Accession DK95468

  18. AcEST: DK953892 [AcEST

    Full Text Available QQACLSSKQLAVRCEGPCPCPTEQSTASTTDS 194 >sp|A6VQ42|RNFC_ACTSZ Electron transport complex protein rnfC OS=Actinobacillus succi...SE Testican-2 OS=Mus musculus GN=Spock2 PE=2 ... 32 2.1 sp|A6VQ42|RNFC_ACTSZ Electron transpo... glutamic... 30 8.1 >sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 SV=1 Length = 423 Score...XLW1|Y2658_SYNP2 PKHD-type hydroxylase SYNPCC7002_A2658 OS=Synechococcus sp. (str...7 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0018_P19. 5' end sequence. Accession DK95389

  19. AcEST: DK951694 [AcEST

    Full Text Available ts: (bits) Value sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 ... 32 1.9 sp|B1XLW1|Y2658_...Homo sapiens GN... 30 9.3 >sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 SV=1 Length = 423...TEQSTASTTDS 194 >sp|B1XLW1|Y2658_SYNP2 PKHD-type hydroxylase SYNPCC7002_A2658 OS=Synechococc...x protein rnfC OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=rnfC PE=3 SV=1 Length = 703 Scor...9 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0012_B10. 5' end sequence. Accession DK95169

  20. AcEST: DK947612 [AcEST

    Full Text Available N2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 ... 32 1.7 sp|A6VQ42|RNFC_ACTSZ Electron transport comple...Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 SV=1 Length = 423 Score = 32.3 bits (72), Expec...rt complex protein rnfC OS=Actinobacillus succinogenes (s...SYNP2 PKHD-type hydroxylase SYNPCC7002_A2658 OS=Synechococcus sp. (strain ATCC 27...8 Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0016_H08. 5' end sequence. Accession DK94761

  1. AcEST: DK954462 [AcEST

    Full Text Available ase SYNPCC7002_A2658 OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) Align length 61 Score (bit) 32.3 E-value 1.9 Repo... >sp|A6VQ42|RNFC_ACTSZ Electron transport complex protein rnfC OS=Actinobacillus succinogenes (strain ATCC 5...N2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 ... 30 9.3 sp|Q9UPW0|FOXJ3_HUM...AN Forkhead box protein J3 OS=Homo sapiens GN... 30 9.3 >sp|B1XLW1|Y2658_SYNP2 PKHD-type hydroxylase SYNPCC7002_A2658 OS=Synechococc...VICEVVEGGP 150 >sp|Q9ER58|TICN2_MOUSE Testican-2 OS=Mus musculus GN=Spock2 PE=2 S

  2. Dicty_cDB: Contig-U13341-1 [Dicty_cDB

    Full Text Available wlesi strain H chro... 112 6e-28 AE014296_2729( AE014296 |pid:none) Drosophila melanogaster chromos...K1) RecName: Full=mRNA-decapping enzyme subunit 2; ... 117 2e-25 AE014296_2730( AE014296 |pid:none) Drosophila melanogaster chromos...e) Oryctes rhinoceros virus isolate ... 68 1e-10 FN357300_1( FN357300 |pid:none) Schistosoma mansoni geno....2 CP000910_1119( CP000910 |pid:none) Renibacterium salmoninarum ATCC... 35 1.2 CP001091_1984( CP001091 |pid:none) Actino... EC=3.6... 35 1.2 CP000687_1884( CP000687 |pid:none) Actinobacillus pleuropneumoniae... 35 1.2 CP001147_219( CP001147 |pid:non

  3. Dicty_cDB: Contig-U09625-1 [Dicty_cDB

    Full Text Available ch... 62 3e-08 CP000481_1283( CP000481 |pid:none) Acidothermus cellulolyticus 11B... 62 3e-08 A96945( A96945 ) 4 animobutyrate amino...Q374002_1( DQ374002 |pid:none) Glossina morsitans morsitans alani... 59 3e-07 AP0...ella pneumoniae subsp. pn... 59 4e-07 CP001192_65( CP001192 |pid:none) Rhizobium leguminos...WO2008... 60 1e-07 ( O66557 ) RecName: Full=Adenosylmethionine-8-amino-7-oxononanoate... 60 1e-07 CP000884_2788( CP000884 |pid:non...om... 59 3e-07 CP000569_1940( CP000569 |pid:none) Actinobacillus pleuropneumoniae... 59 3e-07 AF166351_1( AF166351 |pid:non

  4. Dicty_cDB: Contig-U06348-1 [Dicty_cDB

    Full Text Available e) Sodalis glossinidius str. 'mors... 55 2e-06 CP001102_416( CP001102 |pid:non...... 55 2e-06 AM260479_948( AM260479 |pid:none) Ralstonia eutropha H16 chromosom... 55 2e-06 AP008232_2223( AP008232 |pid:non...11_2009( CU207211 |pid:none) Herminiimonas arsenicoxydans ch... 53 7e-06 CP000958_957( CP000958 |pid:non...CP001091_1911( CP001091 |pid:none) Actinobacillus pleuropneumoniae... 50 4e-05 CP000825_210( CP000825 |pid:non...... 44 5.7 1 ( AP010467 ) Lotus japonicus genomic DNA, chromosome 4, clone:... 44 5.7

  5. Dicty_cDB: Contig-U00515-1 [Dicty_cDB

    Full Text Available ) Cryptococcus neoformans var. neo... 43 0.029 AY194224_1( AY194224 |pid:none) Aedes aegypti strain...a genomic DNA ch... 55 6e-06 BX640431_139( BX640431 |pid:none) Bordetella parapertussis strain...8_635( CU914168 |pid:none) Ralstonia solanacearum strain IP... 39 0.33 CP000687_334( CP000687 |pid:none) Actinobacillus pleuropne.... Toulou... 39 0.56 AJ496288_1( AJ496288 |pid:none) Bartonella tribocorum succinate de... 39 0.56 (Q7S3C9) RecName: Full=Kynurenine...) Ralstonia solanacearum strain Mo... 37 1.2 CP000750_2785( CP000750 |pid:none) Kine

  6. 猪传染性胸膜肺炎的诊治

    田海蓉; 张登祥

    2007-01-01

    猪传染性胸膜肺炎(Porcine Contagious pleuropneu—moniae,PCP)是由胸膜肺炎放线杆菌(Actinobacillus pleurop—neumoniae,APP)引起猪的一种呼吸系统传染病,任何年龄段猪均可发生,经空气或直接接触传播,应激可促使急性暴发,表现为典型的胸膜肺炎、鼻孔分泌物及口腔呕吐物带血。本病对养猪户造成的直接经济损失主要体现在病猪死亡损失和治疗药物费用上。

  7. Transcriptional profiling at different sites in lungs of pigs during acute bacterial respiratory infection

    Mortensen, Shila; Skovgaard, Kerstin; Hedegaard, Jakob; Bendixen, Christian; Heegaard, Peter M. H.

    2011-01-01

    The local transcriptional response was studied in different locations of lungs from pigs experimentally infected with the respiratory pathogen Actinobacillus pleuropneumoniae serotype 5B, using porcine cDNA microarrays. This infection gives rise to well-demarcated infection loci in the lung...... apoptosis and the complement system. Interferon-g was downregulated in both necrotic and bordering areas. Evidence of neutrophil recruitment was seen by the up-regulation of chemotactic factors for neutrophils. In conclusion, we found subsets of genes expressed at different levels in the three selected...... of induced genes as, in unaffected areas a large part of differently expressed genes were involved in systemic reactions to infections, while differently expressed genes in necrotic areas were mainly concerned with homeostasis regulation....

  8. Determination of the antibacterial activity of simvastatin against periodontal pathogens, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans: An in vitro study

    Shilpa Emani

    2014-01-01

    Full Text Available Context and Objective: Statin treatment, apart from its hypolipidemic action has proven its antimicrobial activity by improving the survival rate of patients with severe systemic bacterial infections. Periodontitis is an inflammatory disorder of tooth supporting structures caused by a group of specific microorganisms. The objective of the present study was to determine the antimicrobial activity of pure simvastatin drug against the primary periodontal pathogens. Materials and Methods: Minimum inhibitory concentration (MIC was determined against Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans using serial dilution method. Results: MIC of simvastatin against P. gingivalis was 2 μg/ml and A. actinomycetemcomitans was found to be <1 μg/ml which requires further dilutions to determine the exact value. Conclusions: Data suggests a potent antimicrobial activity of simvastatin against both A. actinomycetemcomitans and P gingivalis. Hence simvastatin can be prescribed as a dual action drug in patients with both hyperlipidemia and periodontal disease.

  9. Dicty_cDB: Contig-U11289-1 [Dicty_cDB

    Full Text Available c clone ZM... 48 0.61 1 ( BZ319034 ) hx52c12.g1 WGS-ZmaysF (JM107 adapted methyl filte... 48 0.61 1 ( EH6658...DNA DKFZp459M2... 222 2e-56 AE004439_277( AE004439 |pid:none) Pasteurella multoci...-59 AL607152_10( AL607152 |pid:none) Mouse DNA sequence from clone RP2... 230 5e-59 AY449460_20( AY449460 |pid:none) Oikopleur... CP000569 |pid:none) Actinobacillus pleuropneumoniae ... 224 3e-57 AK296551_1( AK...001001 |pid:none) Methylobacterium radiotolerans J... 218 2e-55 U41762_1( U41762 |pid:none) Rhodobacte

  10. . and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter aphrophilus to include V factor-dependent and V factor-independent isolates

    Nørskov-Lauritsen, N.; Kilian, Mogens

    2006-01-01

    Haemophilus aphrophilus was able to transform Haemophilus paraphrophilus into the NAD-independent phenotype. The transformants carried a full-length nadV inserted in the former locus of the pseudogene. The DNA-DNA relatedness between the type strains of Haemophilus aphrophilus and Haemophilus paraphrophilus.......73(T)=NCTC 5906(T)) and Aggregatibacter segnis comb. nov. (type strain HK316(T)=ATCC 33393(T)=CCUG 10787(T)=CCUG 12838(T)=CIP 103292(T)=NCTC 10977(T)). The species of the genus Aggregatibacter are independent of X factor and variably dependent on V factor for growth in vitro......The aim of this study was to reinvestigate the relationships and the generic affiliations of the species Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis. The nicotinamide phosphoribosyltransferase gene (nadV) conferring V factor-independent...