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Sample records for aceitera elaeis guineensis

  1. Mortalidad y repelencia en Eupalamides cyparissias (Lepidoptera: Castniidae), plaga de la palma aceitera Elaeis guineensis, por efecto de diez extractos botánicos Mortality and repellence of Eupalamides cyparissias (Lepidoptera: Castniidae), pest of oil palm Elaeis guineensis, by effect of ten botanical extracts

    Diana D. Pérez; José Iannacone O

    2008-01-01

    Las plantas con actividad insecticida constituyen un importante componente del manejo integrado de plagas. Bajo esta premisa, el objetivo del presente estudio fue evaluar la mortalidad y repelencia larval de Eupalamides cyparissias Fab. (Lepidoptera: Castniidae), plaga de la palma aceitera Elaeis guineensis Jacquin; empleando diez plantas con potencial insecticida: Ucullucuysacha (Heliotropium indicum L., Boraginaceae), Floripondio (Brugmansia x candida Pers., Solanaceae), Oreja de Tigre (Tra...

  2. Patogenicidad de Pestalotiopsis palmarum Cooke, sobre plantas de vivero de palma aceitera (Elaeis guineensis Jacq.) Patalotiopsis palmarum Cooke, pathogenicity on nursery-oil palm (Elaeis guineensis Jacq.) plants

    M. Labarca; N. Sanabria; A. Arcia

    2006-01-01

    La palma de aceite (Elaeis guineensis Jacq.), es la oleaginosa que mayor cantidad de aceite produce por superficie, obteniéndose de ella dos tipos de aceite libres de colesterol, que han suplantado en gran parte las grasas animales. La siembra de plantaciones de palma, crea un microclima con condiciones favorables para el desarrollo de plagas y enfermedades. Una de las enfermedades más importantes es la Pestalotiopsis, la cual es causada por los hongos Pestalotiopsis palmarum y Pestalotiopsis...

  3. Dos nuevas especies de lepidópteros defoliadores en plantaciones de palma Aceitera elaeis guineensis jacq. En venezuela Two new lepidoptera species defoliating oil palm plantations Elaeis guineensis Jacq. in Venezuela

    Francisco Sosa; Francisco Díaz

    2008-01-01

    Se realizaron estudios de campo con el objetivo de determinar la presencia de lepidópteros defoliadores en plantaciones de palma aceitera (Elaeis guinnesis Jacq.) en una zona del estado Yaracuy, Venezuela. Para ello se realizaron dos muestreos mensuales desde mayo hasta agosto de 2006. Las diferentes fases insectiles colectadas se colocaron en bolsas plásticas dentro de cavas refrigeradas para luego ser trasladadas al laboratorio. Entre otros defoliadores que atacaban al cultivo, se identific...

  4. SÍNTOMAS ASOCIADOS A LA DEFICIENCIA DE BORO EN LA PALMA ACEITERA (Elaeis guineensis JACQ. EN COSTA RICA

    Marco V. Guti\\u00E9rrez-Soto

    2013-01-01

    Full Text Available El objetivo de este trabajo es brindar información fisiológica relativa a las funciones, la disponibilidad, la economía y la deficiencia de boro en las plantas, con énfasis en la palma aceitera cultivada en Coto, Costa Rica. Se exponen las generalidades sobre la química del boro en el suelo y en las plantas, y se discuten sus posibles funciones estructurales, metabólicas y morfogenéticas. Se resalta su papel en la formación y la estabilidad de la pared celular, el transporte de azúcares, el control de los niveles de auxinas y en el desarrollo reproductivo. Se compara la susceptibilidad relativa de las hojas y las raíces a la deficiencia de boro. Se describen los síntomas de la deficiencia de boro observados en la palma aceitera, los posibles mecanismos causales y los métodos apropiados para su diagnóstico. Se discuten además las terapias, formulaciones químicas y métodos agronómicos disponibles para su corrección.

  5. Mortalidad y repelencia en Eupalamides cyparissias (Lepidoptera: Castniidae, plaga de la palma aceitera Elaeis guineensis, por efecto de diez extractos botánicos Mortality and repellence of Eupalamides cyparissias (Lepidoptera: Castniidae, pest of oil palm Elaeis guineensis, by effect of ten botanical extracts

    Diana D. Pérez

    2008-06-01

    Full Text Available Las plantas con actividad insecticida constituyen un importante componente del manejo integrado de plagas. Bajo esta premisa, el objetivo del presente estudio fue evaluar la mortalidad y repelencia larval de Eupalamides cyparissias Fab. (Lepidoptera: Castniidae, plaga de la palma aceitera Elaeis guineensis Jacquin; empleando diez plantas con potencial insecticida: Ucullucuysacha (Heliotropium indicum L., Boraginaceae, Floripondio (Brugmansia x candida Pers., Solanaceae, Oreja de Tigre (Tradescantia zebrina Hort ex Bosse, Commelinaceae, Piñón Blanco (Jathropa curcas L., Euphorbiaceae, Sacha yoco (Paullinia clavigera Schltdl., Sapindaceae, Yuquilla (Euphorbia cotinifolia L., Euphorbiaceae, Achiote (Bixa orellana L., Bixaceae, Retama común (Cassia fistula L., Fabaceae, Huancahuisacha (Aristolochia pilosa Kunth, Aristolochiaceae y Curare (Chondrodendron tomentosum Ruiz & Pavon, Menispermaceae. Los bioensayos con E. cyparissias abarcaron entre 1 h y 24 h, bajo condiciones estandardizadas de laboratorio. A 24 h de exposición, los mayores porcentajes de mortalidad de E. cyparissias se presentaron en los tratamientos con Sacha yoco (63,3 %: corteza y hojas en decocción, Achiote (63,3 %: semillas en licuado y Yuquilla (48,3 %: hojas en licuado. En el caso de la repelencia, los mayores efectos se encontraron en los tratamientos con Achiote (83,30 %, Sacha yoco (75 % y Floripondio (66,7 %: hojas en licuado.The plants with insecticide activities constitute a main compound of integrated pest management. Under this premise, the aim of the current research was to evaluate mortality and repellence of Eupalamides cyparissias Fab. (Lepidoptera: Castniidae larvae, pest of oil palm Elaeis guineensis Jacquin, employing ten plants with insecticide potential: Indian heliotrope (Heliotropium indicum L., Boraginaceae, Angel´s trumpets (Brugmansia x candida Pers., Solanaceae, Wandering Jew (Tradescantia zebrina Hort ex Bosse, Commelinaceae, Nettles-purge (Jathropa

  6. USO DE WINRHIZO® EN LA CUANTIFICACIÓN DE LAS RAÍCES Y SU APLICACIÓN EN LA PALMA ACEITERA (Elaeis guineensis JACQ.

    Marco V. Guti\\u00E9rrez-Soto

    2014-01-01

    Full Text Available El objetivo de esta nota técnica es introducir el uso de WinRhizo ® para el análisis cuantitativo de muestras del sistema radical de los cultivos en general y de la palma aceitera en particular. Se mencionan las características mor - fológicas, topológicas y funcionales de las raíces de impor - tancia agronómica, y la contribución del análisis cuantitativo realizado por paquetes informáticos especializados como WinRhizo ® . La información sobre la topología, la morfolo - gía, el diámetro, las ramificaciones y el color de las raíces, entre otras variables cuantificadas por WinRhizo ® , es una importante herramienta para el diagnóstico, la prevención y el tratamiento del estrés, y en la evaluación de numerosas prácticas agronómicas como la aplicación de fertilizantes, enmiendas, compostas y coberturas de diversos tipos. Se revisa el procedimiento para el procesamiento de raíces y se indican los ajustes necesarios para la cuantificación de las raíces de la palma aceitera.

  7. Bionomics data and descriptions of the immatures of Calyptocephala gerstaeckeri Boheman (Coleoptera: Chrysomelidae), pest of the oil palm (Elaeis guineensis J.) and camedor palm (Chamaedorea elegans Mart.) (Arecaceae) in Tabasco, Mexico; Datos bionomicos y descripcion de los inmaduros de Calyptocephala gerstaeckeri Boheman (Coleoptera: Chrysomelidae), plaga de la Palma Aceitera (Elaeis guineensis J.) y de la Palma Camedor (Chamaedorea elegans Mart.) (Arecaceae) en Tabasco, Mexico

    Cordova-Ballona, Leonides [Universidad Popular de la Chontalpa, Tabasco (Mexico); Sanchez-Soto, Saul [Colegio de Postgraduados, Tabasco (Mexico). Campus Tabasco

    2008-11-15

    Characters of the external morphology of egg, larval instars and pupae of Calyptocephala gerstaeckeri Boheman, insect pest of oil palm (Elaeis guineensis J.) and camedor palm (Chamaedorea elegans Mart.) in the State of Tabasco, Mexico, are described and illustrated. Some bionomics data are also presented. The specie was reared in the laboratory on young oil palm plants. (author)

  8. Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid

    Camillo, Julceia; Leão, André P; Alves, Alexandre A; Formighieri, Eduardo F; Azevedo, Ana LS; Nunes, Juliana D; de Capdeville, Guy; de A Mattos, Jean K; Souza, Manoel T

    2014-01-01

    Aiming at generating a comprehensive genomic database on Elaeis spp., our group is leading several R&D initiatives with Elaeis guineensis (African oil palm) and Elaeis oleifera (American oil palm), including the whole-genome sequencing of the last. Genome size estimates currently available for this genus are controversial, as they indicate that American oil palm genome is about half the size of the African oil palm genome and that the genome of the interspecific hybrid is bigger than both the...

  9. Wound Healing Activity of Elaeis guineensis Leaf Extract Ointment

    Sreenivasan Sasidharan; Lachimanan Yoga Latha; Selvarasoo Logeswaran

    2011-01-01

    Elaeis guineensis of the Arecaceae family is widely used in the traditional medicine of societies in West Africa for treating various ailments. To validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied. The results showed that E. guineensis leaf extract had potent wound healing capacity as evident from the better wound closure (P < 0.05), improved tissue regeneration at the wound site, and supporting histopathological parameters pertaining to...

  10. Intersepsi Pada Berbagai Kelas Umur Tegakan Kelapa Sawit (Elaeis Guineensis )

    Sonita Fransiska Pelawi

    2009-01-01

    SONITA FRANSISKA PELAWI. Intersepsi pada berbagai kelas umur tegakan kelapa sawit (Elaeis guineensis). Dibawah bimbingan BEJO SLAMET dan ACHMAD SIDDIK THOHA. Konversi hutan menjadi perkebunan, lahan pertanian dan pemukiman mempengaruhi kondisi hidrologi suatu kawasan Daerah Aliran Sungai (DAS). Penelitian ini bertujuan untuk mengetahui besarnya intersepsi dan mendapatkan hubungan curah hujan dengan intersepsi pada tegakan kelapa sawit berbagai kelas umur. Penelitian ini dilaksanakan di PT...

  11. Wound Healing Activity of Elaeis guineensis Leaf Extract Ointment

    Sreenivasan Sasidharan

    2011-12-01

    Full Text Available Elaeis guineensis of the Arecaceae family is widely used in the traditional medicine of societies in West Africa for treating various ailments. To validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied. The results showed that E. guineensis leaf extract had potent wound healing capacity as evident from the better wound closure (P < 0.05, improved tissue regeneration at the wound site, and supporting histopathological parameters pertaining to wound healing. Matrix metalloproteinases expression correlated well with the results thus confirming efficacy of E. guineensis in the treatment of the wound. E. guineensis accelerated wound healing in rats, thus supporting its traditional use. The result of this study suggested that, used efficiently, oil palm leaf extract is a renewable resource with wound healing properties.

  12. Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid.

    Camillo, Julceia; Leão, André P; Alves, Alexandre A; Formighieri, Eduardo F; Azevedo, Ana Ls; Nunes, Juliana D; de Capdeville, Guy; de A Mattos, Jean K; Souza, Manoel T

    2014-01-01

    Aiming at generating a comprehensive genomic database on Elaeis spp., our group is leading several R&D initiatives with Elaeis guineensis (African oil palm) and Elaeis oleifera (American oil palm), including the whole-genome sequencing of the last. Genome size estimates currently available for this genus are controversial, as they indicate that American oil palm genome is about half the size of the African oil palm genome and that the genome of the interspecific hybrid is bigger than both the parental species genomes. We estimated the genome size of three E. guineensis genotypes, five E. oleifera genotypes, and two interspecific hybrids genotypes. On average, the genome size of E. guineensis is 4.32 ± 0.173 pg, while that of E. oleifera is 4.43 ± 0.018 pg. This indicates that both genomes are similar in size, even though E. oleifera is in fact bigger. As expected, the hybrid genome size is around the average of the two genomes, 4.40 ± 0.016 pg. Additionally, we demonstrate that both species present around 38% of GC content. As our results contradict the currently available data on Elaeis spp. genome sizes, we propose that the actual genome size of the Elaeis species is around 4 pg and that American oil palm possesses a larger genome than African oil palm. PMID:26203259

  13. Kemampuan AntiFungi Bakteri Endofit Kelapa Sawit (Elaeis guineensis Jacq.) Terhadap Ganoderma boninenese Pat

    Simbolon, Dewi Novelina

    2010-01-01

    Penelitian tentang “Kemampuan Antifungi Bakteri Endofit Kelapa Sawit (Elaeis guineensis Jack. Terhadap Ganoderma boninense Pat. telah dilakukan di Laboratorium Mikrobiologi, Departemen Biologi, FMIPA USU. Tujuan dari penelitian ini adalah untuk mengetahui mekanisme penghambatan bakteri endofit kelapa sawit (Elaeis guineensis Jacq) terhadap G. boninense. Tiga bakteri endofit yang berpotensi antagonis dideteksi kemampuan kitinolitik, glukanolitik dan lisis terhadap G. boninense. Dari hasil kiti...

  14. BIOCHEMICAL AND PHYSIOLOGICAL CHARACTERIZATION OF OIL PALM INTERSPECIFIC HYBRIDS (Elaeis oleifera x Elaeis guineensis) GROWN IN HYDROPONICS

    Yurany Dayana Rivera; Andrés Leonardo Moreno Chacón; Hernán Mauricio Romero

    2013-01-01

    The interspecific hybrid, Elaeis oleifera x Elaeis guineensis (OxG) is an alternative for improving the competitiveness and sustainability of the Latin American oil palm agro-industry, because of its partial resistance to some lethal diseases and also because of the high quality of its oil. A comparative characterization was conducted of the physiological and biochemical performance of seedlings of six OxG hybrids grown in hydroponics. Gas exchange, vegetative growth, protein, sugar and photo...

  15. In vitro protocol optimization for development of interspecific hybrids of oil palm (Elaeis oleifera (H.B.K) Cortés x Elaeis guineensis Jacq.)

    Sergio Augusto Oliveira Alves; Oriel Filgueira de Lemos; Benedito Gomes dos Santos Filho; André Luís Lopes da Silva

    2011-01-01

    The oil palm (Elaeis guineensis) is the major source of plant oil in the world. Nevertheless, is susceptible to the fatalyellowing disease, which is affecting the oil palm production in Pará state, Brazil. To overcome this problem,interspecific hybrids of Elaeis oleifera x Elaeis guineensis is a viable alternative. Some protocols for in vitro embryorescue were already established, but it is necessary to optimize it. The aim of this paper was to optimize the in vitroprotocol for culture of emb...

  16. Determination of Antioxidants in Oil Palm Leaves (Elaeis guineensis

    Ng M. Han

    2010-01-01

    Full Text Available Problem statement: Previous findings on the occurrence of water soluble antioxidants in palm oil has brought to the question on whether these compounds is also present in other parts of the oil palm; namely its leaves. Approach: It is now believed that the water soluble antioxidants are also present in other biomass of the oil palm, namely, the leaves. This study reported on the determination of the water soluble antioxidants in oil palm leaves. Results: The results showed the analyses of the antioxidants in oil palm leaves. Conclusion: This study is thus conducted to trace the availability of these antioxidants in the leaves of the oil palm of the Elaeis guineensis variety.

  17. In vitro development and acclimatization of dendezeiro (Elaeis guineensis

    Marlúcia Souza Souza Pádua

    2014-12-01

    Full Text Available Fruits and almond from the dendezeiro, oil palmbelonging to the Elaeis genus,are widely used for the production of cookingoils or for the pharmaceutical and cosmetic industries.In the last decade, this oil palm also emerged as a promising source for commercialbiofuel production. This study evaluated the effect of different culture media, MS (MURASHIGUE AND SKOOG and Y3 (EEUWENSand carbohydrates duringin vitro germination of zygotic embryos, the effect of growth regulators GA3, NAA and BA Ponin vitro seedling development, and the survival rate of acclimatized seedlingsof Manicoré hybrid (Elaeis oleifera x E. guineensis. Zygotic embryos were inoculated on MS and modified Y3 media, supplemented with different sucrose concentrations (30, 45, and 60 gL-1 or sorbitol (36 gL-1, and the germination rate was evaluated after 30 days. Subsequently, seedlings were transferred to modified Y3 culture medium supplemented with differentGA3 concentrations (3.5 and 7 mgL-1 or without it, combined or not with 1 mgL-1 of NAA, 5 mgL-1 of BAP.The highest germinationpercentage of germinated embryos (92% was observed in MS medium supplemented with 36 gL-1 sorbitol. Culture media supplemented with growth regulatorsGA3, NAA and BAP promoted greater shoot lengththan control media. Rooted seedlings showed high survival percentage (85% during acclimatization.

  18. The Antimicrobial Efficacy of Elaeis guineensis: Characterization, in Vitro and in Vivo Studies

    Sreenivasan Sasidharan; Kanwar, Jagat R.; Lachimanan Yoga Latha; Zuraini Zakaria; Yeng Chen; Soundararajan Vijayarathna

    2012-01-01

    The urgent need to treat multi-drug resistant pathogenic microorganisms in chronically infected patients has given rise to the development of new antimicrobials from natural resources. We have tested Elaeis guineensis Jacq (Arecaceae) methanol extract against a variety of bacterial, fungal and yeast strains associated with infections. Our studies have demonstrated that E. guineensis exhibits excellent antimicrobial activity

  19. Acute Oral Toxicity and Brine Shrimp Lethality of Elaeis guineensis Jacq., (Oil Palm Leaf) Methanol Extract

    Yeng Chen; Lai Ngit Shin; Yee Ling Lau; Sreenivasan Sasidharan; Yuet Ping Kwan; Lachimanan Yoga Latha; Soundararajan Vijayarathna; Abdul Rani Muhamad Syahmi

    2010-01-01

    Elaeis guineensis (Arecaceae) is widely used in West African traditional medicine for treating various ailments. An evaluation on the toxicity of extracts of this plant is crucial to support the therapeutic claims. The acute oral toxicity and brine shrimp lethality of a methanolic extract of this plant was tested. Oral administration of crude extract at the highest dose of 5,000 mg/kg resulted in no mortalities or evidence of adverse effects, implying that E. guineensis is nontoxic. Normal be...

  20. Biochemical characterisation during seed development of oil palm (Elaeis guineensis).

    Kok, Sau-Yee; Namasivayam, Parameswari; Ee, Gwendoline Cheng-Lian; Ong-Abdullah, Meilina

    2013-07-01

    Developmental biochemical information is a vital base for the elucidation of seed physiology and metabolism. However, no data regarding the biochemical profile of oil palm (Elaeis guineensis Jacq.) seed development has been reported thus far. In this study, the biochemical changes in the developing oil palm seed were investigated to study their developmental pattern. The biochemical composition found in the seed differed significantly among the developmental stages. During early seed development, the water, hexose (glucose and fructose), calcium and manganese contents were present in significantly high levels compared to the late developmental stage. Remarkable changes in the biochemical composition were observed at 10 weeks after anthesis (WAA): the dry weight and sucrose content increased significantly, whereas the water content and hexose content declined. The switch from a high to low hexose/sucrose ratio could be used to identify the onset of the maturation phase. At the late stage, dramatic water loss occurred, whereas the content of storage reserves increased progressively. Lauric acid was the most abundant fatty acid found in oil palm seed starting from 10 WAA. PMID:23575803

  1. Uji Aktivitas Antioksidan Minyak Serat Mesokarp Buah Kalapa Sawit (Elaeis Guineensis Jacq)

    Pandiangan, Michio Anataki

    2015-01-01

    Serat mesokarp buah kelapa sawit memiliki kandungan minyak dan karoten yang sangat tinggi. Kandungan karoten yang tinggi di dalam minyak dapat dimanfaatkan sebagai antioksidan. Pusat penelitian kelapa sawit telah mengembangkan proses ekstraksi karoten untuk meningkatkan konsentrasinya. Variasi proses dalam ekstraksi karoten dapat mempengaruhi aktivitas antioksidan. Tujuan penelitian ini untuk menguji aktivitas antioksidan serat mesokarp buah kelapa sawit (Elaeis guineensis Jacg). Karoten ...

  2. Models to estimate the bunch dry weight in african oil palm (elaeis guineensis jacq.), american oil palm (elaeis oleifera h.b.k. cortes) and the interspecific hybrid (e. oleifera x e. guineensis)

    2012-01-01

    Statistical models were used to estimate the bunch dry weight through indirect nondestructive methods in African oil palm (Elaeis guineensis) American oil palm (Elaeis oleifera) and the interspecific hybrid (E. oleifera x E. guineensis); and compared with the formula proposed by Corley. The studies were conducted at Santa Barbara and Chaparral-Cuernavaca on the Unipalma plantation, located in the eastern palm region of Colombia. Ten palms were selected for each group and 30 bunches were sampl...

  3. Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and Vero cell lines

    Soundararajan; Vijayarathna; Sreenivasan; Sasidharan

    2012-01-01

    Objective:To investigate the cytotoxic effect of Elaeis guineensis methanol extract on MCF-7and Vero cell.Methods:In vitro cytotoxicity was evaluated in by MTT assay.Cell morphological changes were observed by using light microscope.Results:The MTT assay indicated that methanol extract of the plant exhibited significant cytotoxic effects on MCF-7.Morphological alteration of the cell lines after exposure with lilaeis guineensis extract were observed under phase contrast microscope in the dose dependent manner.Conclusions:The results suggest the probable use of the Elaeis guineensis methanol extract in preparing recipes for cancer-related ailments.Further studies on isolation of metabolites and their in vivo cytotoxicity are under investigation.

  4. Characterisation of copia-like retrotransposons in oil palm ( Elaeis guineensis Jacq.).

    Price, Z.; Dumortier, F.; MacDonald, W.; Mayes, S.

    2002-04-01

    The work aimed to isolate and characterise copia-like sequences from Elaeis guineensis. Thirty-two different RT (reverse transcriptase) sequences were isolated from a single oil palm genome using degenerate primers. Extreme sequence heterogeneity was observed. The DNA and protein sequences were assigned to three different classes (A, B, C) on the basis of bootstrapping. We estimated the copy number of the three different classes by using a dot-blot analysis. The comparative results suggest that class-B RT sequences occur at a higher copy number in Cocos nucifera than in Elaeis guineensis and E. oleifera. Class-C RT sequences, which comprise the bulk of isolated sequences, occur in much higher copy number in Elaeis guineensis than in Cocos nucifera, and E. oleifera. Class-A sequences, which have low copy number in Elaeis guineensis and Cocos nucifera, are not present in E. oleifera. Our preliminary results suggest that class-C sequences represent about 5% of the E. guineensis genome, class B, 1% and class A, 0.1%. The methylation status of genomic domains specified by the RT probes was analysed using two pairs of restriction enzymes, each pair having the same recognition sites but different methylation sensitivities ( MspI, HpaII and Sau3AI, MboI). Results from these experiments showed clearly that the genomic domains specified by the RT probes are methylated. These also suggest that a higher copy number appears to correlate to a higher degree of methylation. Our preliminary results suggest that copia-like retrotransposons, because of their ubiquity and diversity, have great potential as genetic markers for plant genome and biodiversity analysis in E. guineensis. PMID:12582647

  5. Skrining Fitokimia dan Uji Efektivitas Sediaan Gel Ekstrak Etanol Daun Kelapa Sawit (Elaeis guineensis Jacq.) Terhadap Penyembuhan Luka Sayat

    Hasibuan, Cinta Suci

    2015-01-01

    Oil palm (Elaeis guineensis Jacq.) leaf family Arecaceae widely growing in Indonesia, which is common known with its oil. One of the medical using is for wound healing, and also effective for hepatoprotective, antihypertensive, antidiabetic, and can heal acut toxicity. The objective of this research is to test phytochemicals screening and effectiveness test ethanol extract gel of oil palm leaf (elaeis guineensis jacq.) for wound healing. Palm’s leaf powder macerated by ethanol 80% for 5 d...

  6. In vitro embryo rescue of interspecific hybrids of oil palm (Elaeis oleifera x Elaeis guineensis)

    Sergio Augusto Oliveira Alves; Oriel Filgueira de Lemos; Benedito Gomes dos Santos Filho; André Luís Lopes da Silva

    2011-01-01

    The oil palm (Elaeis guineennsis Jacq.) is the plant with major oil yield in tropical areas over the world. Pará state is the major producer of Brazil. However, the production of resistant hybrids (Elaeis oleifera x Elaeis guinennsis) to several diseases is necessary, in spite of less productive, is a viable alternative for areas where occur more aggressive diseases. The aim of this research was to rescue in vitro embryos of four varieties of interspecific hybrids of oil palm (Elaeis oleifera...

  7. QTL detection by multi-parent linkage mapping in oil palm (Elaeis guineensis Jacq.).

    Billotte, N.; Jourjon, M-Francoise; Marseillac, N.; Berger, A; Flori, A.; Asmady, H.; Adon, B.; Singh, R.; Nouy, B.; Potier, F.; Cheah, S.C.; Rohde, W; Ritter, E; Courtois, B.; Charrier, A.

    2010-01-01

    A quantitative trait locus (QTL) analysis designed for a multi-parent population was carried out and tested in oil palm (Elaeis guineensis Jacq.), which is a diploid cross-fertilising perennial species. A new extension of the MCQTL package was especially designed for crosses between heterozygous parents. The algorithm, which is now available for any allogamous species, was used to perform and compare two types of QTL search for small size families, within-family analysis and across-family ana...

  8. Beneficial of activated charcoal on embryo culture of oil palm (Elaeis guineensis Jacq.)

    Kamnoon Kanchanapoom; Boonsanong Chourkaew; Wisut Patcharapisutsin

    2001-01-01

    Mature embryos of oil palm (Elaeis guineensis Jacq.)variety Tenera were cultured on Eeuwens(1976) or Y3 medium without plant growth regulators and supplemented with 0.05% activated charcoal (AC).Shoots with well-developed roots were produced on the medium. It was found that AC improved growth of seedlings. The effect of AC containing media is discussed. The embryos were fixed, sectioned, stained and examined microscopically. Anatomical study revealed that the morphological organization of oil...

  9. Characterization of Different Parts of Oil Palm Fronds (Elaeis Guineensis) and Its Properties

    Noor Afeefah Nordin; Othman Sulaiman; Rokiah Hashim; Mohamad Haafiz Mohamad Kassim

    2016-01-01

    The study was conducted on characterizing the properties of oil palm fronds (Elaeis guineensis) from different parts. The fronds were cut to three parts which were the top, middle and bottom. The samples were chipped, dried in the oven of 50 °C and ground before they were characterized. The properties of the fronds were characterized using thermal gravimetric analyser (TGA), fourier transform infrared spectroscopy (FTIR) and x-ray diffraction (XRD). The results from TGA showed that the bottom...

  10. Uji Aktivitas Ekstrak Etanol Daun Kelapa Sawit Sebagai Antikolesterol (Elaeis guineensis Jacq.) Menggunakan Mencit Jantan

    Syahfitri, Rifany

    2016-01-01

    Indonesia is one of the largest producer oil palm (Elaeis guineensis Jacq.) in the world. Palm leaves are generally only as waste, but the development of science and technology can be used for the treatment of some diseases, such as antibacterial, antioxidant, antihypertensive, antidiabetic, hepatoprotective, acute toxicity, wound healing drug. The purpose of this study was to determine the activity of the ethanol extract of palm leaves (EOPLE) as anti-cholesterol using male mice. Simplici...

  11. Pengujian Sediaan Gel Ekstrak Etanol Daun Kelapa Sawit(Elaeis guineensis Jacq.) Sebagai Obat Luka Bakar

    Wahyudi

    2016-01-01

    Leaves of palm (Elaeis guineensis Jacq.) Contain alkaloids that have the ability as an antibacterial, astringent activity of flavonoids and saponins that can stimulate the formation of collagen, which plays a role in wound healing process and. This study aimed to test the effectiveness of the ethanol extract gel palm leaves for the healing of burns. Palm’ leaf powder macerated by ethanol 80% for 5 days, filtered, the residue has extraction by ethanol, then the filtrate leave for 2 day and ...

  12. Analisis Histologi Embriogenesis Somatik Dari Apikal Bud Kelapa Sawit (Elaeis guineensis Jacq.) var Tenera

    Meilvana, Tengku Nilayanda

    2014-01-01

    A study of Histological Analysis of Somatic Embryogenesis from Apical Bud of Oil Palm (Elaeis guineensis Jacq.) var. Tenera has been conducted at Plant Tissue Culture Laboratory of FMIPA USU. The experimental design was completely randomized with three levels of 2,4-D concentrations: 110; 120; 130 mg/L and three position of explants: apical, median, and basal. The statistical analysis showed that 120 mg/L of 2,4-D significantly affected the primary and embryogenic calluses initiation. Explant...

  13. De novo transcriptome analyses of host-fungal interactions in oil palm (Elaeis guineensis Jacq.)

    Ho, Chai-Ling; Tan, Yung-Chie; Yeoh, Keat-Ai; Ghazali, Ahmad-Kamal; Yee, Wai-Yan; Hoh, Chee-Choong

    2016-01-01

    Background Basal stem rot (BSR) is a fungal disease in oil palm (Elaeis guineensis Jacq.) which is caused by hemibiotrophic white rot fungi belonging to the Ganoderma genus. Molecular responses of oil palm to these pathogens are not well known although this information is crucial to strategize effective measures to eradicate BSR. In order to elucidate the molecular interactions between oil palm and G. boninense and its biocontrol fungus Trichoderma harzianum, we compared the root transcriptom...

  14. Genetic variation and phylogenetic relationships in oil palm (Elaeis guineensis Jacq.) based on RAPD analysis

    Nualsri, C.; Junmag, S.; Eksomtramage, T.

    2005-01-01

    The genetic variability and phylogenetic relationships in oil palm (Elaeis guineensis Jacq.) were studied using RAPD (Random Amplified Polymorphic DNA). Leaf samples of 151 plants were collected from different areas in southern Thailand. DNA from the leaf samples was isolated using CTAB buffer and screened by decamer oligonucleotide primers. Among the total of 160 primers screened, 7 primers (OPB-08, OPR-11, OPT-06, OPT-19, OPAB-01, OPAB-09 and OPAB-14) were chosen to analyse for genetic vari...

  15. Collection of Oil Palm (Elaeis guineensis Jacq.) Germplasm in the Northern Regions of Ghana

    Sapey, E; K. Adusei-Fosu; D. Agyei-Dwarko; G. Okyere-Boateng

    2012-01-01

    Oil palm germplasm collection was carried out in the Northern Regions of Ghana for evaluation, screening for drought tolerance and further incorporation into breeding programmes of Ghana’s Council for Scientific and Industrial Research (C.S.I.R)-Oil Palm Research Institute (O.P.R.I). The study highlights the collection of 22 oil palm (Elaeis guineensis) accessions from 5 locations in the Northern Regions of Ghana. The Northern Regions are not suitable for oil palm cultivation due to unfavoura...

  16. Quantitative trait loci (QTLs) analysis of palm oil fatty acid composition in an interspecific pseudo-backcross from Elaeis oleifera (HBK) Cortés and oil palm (Elaeis guineensis Jacq.)

    Montoya, Carmenza; Lopes, Ricardo; Flori, Albert; Cros, David; Cuellar, Teresa; Summo, Maryline; Espeout, Sandra; Rivallan, Ronan; Risterucci, Ange-Marie; Bittencourt, Daniela; Zambrano, Jorge R.; Alarcon G, Wilmar H.; Villeneuve, Pierre; Pina, Michel; Nouy, Bruno

    2013-01-01

    We chose an Elaeis interspecific pseudo-backcross of first generation (E. oleifera x E. guineensis) x E. guineensis to identify quantitative trait loci (QTLs) for fatty acid composition of palm oil. A dense microsatellite linkage map of 362 loci spanned 1.485 cM, representing the 16 pairs of homologous chromosomes in the Elaeis genus from which we traced segregating alleles from both E. oleifera and E. guineensis grandparents. The relative linear orders of mapped loci suggested the probable a...

  17. Crude palm oil from interspecific hybrid Elaeis oleifera×Elaeis guineensis: fatty acid regiodistribution and molecular species of glycerides.

    Mozzon, Massimo; Pacetti, Deborah; Lucci, Paolo; Balzano, Michele; Frega, Natale Giuseppe

    2013-11-01

    The composition and structure of triacylglycerols (TAGs) and partial glycerides of crude palm oil obtained from interspecific hybrid Elaeis oleifera×Elaeis guineensis, grown in Colombia, were fully characterised and compared to data obtained by analysing crude African palm oil. Hybridisation appears to substantially modify the biosynthesis of fatty acids (FAs) rather than their assembly in TAGs. In fact, total FAs analysis showed significant differences between these two types of oil, with hybrid palm oil having a higher percentage of oleic acid (54.6 ± 1.0 vs 41.4 ± 0.3), together with a lower saturated fatty acid content (33.5 ± 0.5 vs 47.3 ± 0.1), while the percentage of essential fatty acid, linoleic acid, does not undergo significant changes. Furthermore, 34 TAG types were identified, with no qualitative differences between African and E. guineensis×E. oleifera hybrid palm oil samples. Short and medium chain FAs (8:0, 10:0, 12:0, 14:0) were utilised, together, to build a restricted number of TAG molecular species. Oil samples from the E. guineensis×E. oleifera hybrid showed higher contents of monosaturated TAGs (47.5-51.0% vs 36.7-37.1%) and triunsaturated TAGs (15.5-15.6% vs 5.2-5.4%). The sn-2 position of TAGs in hybrid palm oil was shown to be predominantly esterified with oleic acid (64.7-66.0 mol% vs 55.1-58.2 mol% in African palm oil) with only 10-15% of total palmitic acid and 6-20% of stearic acid acylated in the secondary position. The total amount of diacylglycerols (DAGs) was in agreement with the values of free acidity; DAG types found were in agreement with the representativeness of different TAG species. PMID:23768354

  18. In vitro development and acclimatization of dendezeiro (Elaeis guineensis)

    Marlúcia Souza Souza Pádua; Luciano Viela Paiva; Luis Gustavo Texeira da Silva; Luciano Coutinho Silva; Vanessa Cristina Stein

    2014-01-01

    Fruits and almond from the dendezeiro, oil palmbelonging to the Elaeis genus,are widely used for the production of cookingoils or for the pharmaceutical and cosmetic industries.In the last decade, this oil palm also emerged as a promising source for commercialbiofuel production. This study evaluated the effect of different culture media, MS (MURASHIGUE AND SKOOG) and Y3 (EEUWENS)and carbohydrates duringin vitro germination of zygotic embryos, the effect of growth regulators GA3, NAA and BA Po...

  19. Total amino acid profiles of heat-processed fresh Elaeis guineensis and Raphia hookeri wines.

    Ibegbulem, C O; Igwe, C U; Okwu, G N; Ujowundu, C O; Onyeike, E N; Ayalogu, E O

    2013-06-01

    Total amino acid (AA) profiles of heat-processed fresh Elaeis guineensis and Raphia hookeri wines were studied. Heating their fresh wines to 85°C, cooling and diluting to original volumes distilled off ethanol, but did not change their moisture and nitrogen contents. R. hookeri wine contained more (p0.05). Glycine and Pro contents were low suggesting high globular protein concentrations. ∑basic AA/∑acidic AA ratios were >1 suggesting high basic protein contents. The E. guineensis and R. hookeri wines contained 58.25 ± 0.56% and 56.79 ± 0.4% essential AAs, respectively. Essential AA scores suggested Leu as their limiting AA. In conclusion, the wines can adequately meet daily nitrogen and essential AA needs when a 70 kg adult drinks 1425.45 ml. PMID:23411289

  20. Embryogenic calli induced in interspecific (Elaeis guineensis x E. oleifera hybrid zygotic embryos

    Paula Cristina da Silva Angelo

    2009-01-01

    Full Text Available The hybridization between oil palm (Elaeis guineensis and caiaué (E. oleifera plants is directed to obtainprogenies presenting high yields like oil palm but with reduced shoot height and resistance to lethal yellowing like caiaué.Cloning F1, BC1 and BC2 progenies can make the replication of selection trials easier. The objective of this work was to inducesomatic embryogenesis in interspecific zygotic embryos collected 100 days after pollination. Three progenies were cultivatedin an induction medium developed for Tenera (E. guineensis tp. dura x pisifera embryos. The number of embryos bearing calliand germinating was recorded and submitted to the Z test. Calli were weighted and submitted to histological analysis.Progenies differed in the number of embryos presenting plumules and calli simultaneously. By the ninth month, the apices ofincompletely developed somatic embryos were observed protruding from the surfaces of nodular calli. Highly embryogenicand friable secondary calli producing globular somatic embryos were not observed.

  1. Histology of embryoid development in oil palm (Elaeis guineensis Jacq.) cell suspension culture

    Songrat Tinnongjig; Kamnoon Kanchanapoom

    2001-01-01

    Embryos of oil palm (Elaeis guineensis Jacq.) variety tenera were cultured on Eeuwens or Y3 (1976; 1978) medium supplemented with 2 mg/l 2,4-D. Calluses were initiated from these embryos. The eight-weekold calluses derived from embryos were transferred to modified Y3 liquid medium devoid of 2,4-D and supplemented with NAA, BA and coconut water to establish cell suspension culture. After a period of culture,these cells were then subcultured to the same medium without plant growth regulators to...

  2. ASSESSMENT OF NIGERIAN WILD OIL PALM (ELAEIS GUINEENSIS JACQ.) POPULATIONS IN CROSSES WITH DELI TESTERS

    Noumouha E. N. Ghislain; Allou Désiré; Adon Benjamin; Konan Jean-Noël; Sékou Diabaté; Konan K. Eugène; Simon-Pierre A Nguetta

    2014-01-01

    To widen the genetic variability and especially to enrich agronomic qualities of populations of oil palm (Elaeis guineensis Jacq.) of Group B used in the breeding scheme, a study was led on four populations (Abak, Ahoada, Ayangba and Uli) prospected in Nigeria. Sixteen palms chosen in these populations were evaluated in crosses with 10 Deli testers derived from the second cycle of reciprocal recurrent selection scheme. The LM 2 T × DA 10 D and LM 2 T × DA 115 D progenies derived from the firs...

  3. PENGARUH PEMUPUKAN FOSFOR DAN KALIUM TERHADAP PERTUMBUHAN TANAMAN KELAPA SAWIT (Elaeis Guineensis Jacq.) DI PEMBIBITAN UTAMA

    Sudradjat -; Nindyta Agustina Siagian

    2014-01-01

    Oil palm (Elaeis guineensis Jacq.) is one of plantation commodities that contribute to foreign exchange. Palm oil production is influenced by the expansion of the area  and  intensification using the fertilization at main nursery and plantation. Macro elements such as N, P, and K required by the oil palm are in large quantities. The study was conducted from November, 2011 to  May, 2012 at Cikabayan Experimental Station, IPB Darmaga Campus, Bogor. The treatment was laid-out in a factorial rand...

  4. THE DIVERSITY OF EPIPHYTIC FERN ON THE OIL PALM TREE (Elaeis guineensis Jacq. IN PEKANBARU, RIAU

    Nery Sofiyanti

    2015-02-01

    Full Text Available Oil palm (Elaeis guineensis is one  main commodity in Riau Province. Morphologically, the trunk of oil palm  has suitable environment for the growth of epiphytic fern, due to its broaden base of petiole that may accumulate organic and anorganic debrish. The objective of this study was to investigate the diversity of epiphytic fern on the oil palm tree. A total of 125 oil palm trees from seven  study sites in Pekanbaru, Riau were observed. The number of epiphytic ferns identified in this study was 16 species belongs to six families.

  5. THE DIVERSITY OF EPIPHYTIC FERN ON THE OIL PALM TREE (Elaeis guineensis Jacq.) IN PEKANBARU, RIAU

    Nery Sofiyanti

    2015-01-01

    Oil palm (Elaeis guineensis) is one  main commodity in Riau Province. Morphologically, the trunk of oil palm  has suitable environment for the growth of epiphytic fern, due to its broaden base of petiole that may accumulate organic and anorganic debrish. The objective of this study was to investigate the diversity of epiphytic fern on the oil palm tree. A total of 125 oil palm trees from seven  study sites in Pekanbaru, Riau were observed. The number of epiphytic ferns identified in this stud...

  6. Kepadatan Jumlah Kumbang Badak (Oryctes rhinoceros L.) Pada Tanaman Kelapa Sawit (Elaeis guineensis jacq.) Di Lapangan

    Harahap, Rudini

    2010-01-01

    Rudini Harahap "TOTAL BEETLE density rhinos (Oryctes rhinoceros L.) PLANTATION IN OIL PALM (Elaeis guineensis Jacq.) Field." With the commission supervising Mother Ir. Marheni, MP as chairman and Mr. Ir. Syahrial Oemry, MS, as members.This research was carried out in the garden and the garden Rambutan Padang Huta PTPN III, North Sumatra from April to May 2010.The aim study is to investigate the density of O.rhinoceros in Immature (TBM) diareal using Mucuna and to determine the density of O.rh...

  7. Acute Oral Toxicity and Brine Shrimp Lethality of Elaeis guineensis Jacq., (Oil Palm Leaf Methanol Extract

    Yeng Chen

    2010-11-01

    Full Text Available Elaeis guineensis (Arecaceae is widely used in West African traditional medicine for treating various ailments. An evaluation on the toxicity of extracts of this plant is crucial to support the therapeutic claims. The acute oral toxicity and brine shrimp lethality of a methanolic extract of this plant was tested. Oral administration of crude extract at the highest dose of 5,000 mg/kg resulted in no mortalities or evidence of adverse effects, implying that E. guineensis is nontoxic. Normal behavioral pattern, clinical signs and histology of vital organs confirm this evidence. The E. guineensis extracts screened for toxicity against brine shrimp had 50% lethal concentration (LC50 values of more than 1.0 mg/mL (9.00 and 3.87 mg/mL, at 6 and 24 h, respectively, confirming that the extract was not toxic. Maximum mortalities occurred at 100 mg/mL concentration while the least mortalities happened to be at 0.195 mg/mL concentration. The results of both tests confirm that E. guineensis is nontoxic and hence safe for commercial utilization.

  8. The Antimicrobial Efficacy of Elaeis guineensis: Characterization, in Vitro and in Vivo Studies

    Sreenivasan Sasidharan

    2012-04-01

    Full Text Available The urgent need to treat multi-drug resistant pathogenic microorganisms in chronically infected patients has given rise to the development of new antimicrobials from natural resources. We have tested Elaeis guineensis Jacq (Arecaceae methanol extract against a variety of bacterial, fungal and yeast strains associated with infections. Our studies have demonstrated that E. guineensis exhibits excellent antimicrobial activity in vitro and in vivo against the bacterial and fungal strains tested. A marked inhibitory effect of the E. guineensis extracts was observed against C. albicans whereby E. guineensis extract at ½, 1, or 2 times the MIC significantly inhibited C. albicans growth with a noticeable drop in optical density (OD of the bacterial culture. This finding confirmed the anticandidal activity of the extract on C. albicans. Imaging using scanning (SEM and transmission (TEM electron microscopy was done to determine the major alterations in the microstructure of the extract-treated C. albicans. The main abnormalities noted via SEM and TEM studies were the alteration in morphology of the yeast cells. In vivo antimicrobial activity was studies in mice that had been inoculated with C. albicans and exhibited good anticandidal activity. The authors conclude that the extract may be used as a candidate for the development of anticandidal agent.

  9. Cytokinin Differences in In Vitro Cultures and Inflorescences from Normal and Mantled Oil Palm (Elaeis guineensis Jacq.)

    Ooi, S. E.; Novák, Ondřej; Doležal, Karel; Ishak, Z.; Ong-Abdullah, M.

    2013-01-01

    Roč. 32, č. 4 (2013), s. 865-874. ISSN 0721-7595 Grant ostatní: GA MŠk(CZ) ED0007/01/01 Institutional research plan: CEZ:AV0Z50380511 Keywords : Cytokinin * Elaeis guineensis * Inflorescence Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.058, year: 2013

  10. BIOCHEMICAL AND PHYSIOLOGICAL CHARACTERIZATION OF OIL PALM INTERSPECIFIC HYBRIDS (Elaeis oleifera x Elaeis guineensis GROWN IN HYDROPONICS

    Yurany Dayana Rivera

    2013-09-01

    Full Text Available The interspecific hybrid, Elaeis oleifera x Elaeis guineensis (OxG is an alternative for improving the competitiveness and sustainability of the Latin American oil palm agro-industry, because of its partial resistance to some lethal diseases and also because of the high quality of its oil. A comparative characterization was conducted of the physiological and biochemical performance of seedlings of six OxG hybrids grown in hydroponics. Gas exchange, vegetative growth, protein, sugar and photosynthetic pigment content, and antioxidant system activity were determined. With the exception of gas exchange, the other variables showed significant differences between materials. The ‘U1273’ and ‘U1737’ materials showed greater vegetative growth with no expression of biochemical traits, while the ‘U1914’and ‘U1990’ materials showed high levels of reducing and total sugars, photosynthetic pigments, and antioxidant system activities, characteristics that could confer them adaptation to stress conditions. With the standardized hydroponics technique, the optimal conditions for the growth of seedlings were ensured, the differences between materials and hybrid crosses were established, so those with promising features from the physiological and biochemical standpoint were identified. Finally, it could be used to study in a simple, fast, clean and inexpensive way, the effect of levels and sources of mineral nutrients on the growth and development of oil palm.

  11. PENGARUH PEMUPUKAN FOSFOR DAN KALIUM TERHADAP PERTUMBUHAN TANAMAN KELAPA SAWIT (Elaeis Guineensis Jacq. DI PEMBIBITAN UTAMA

    Sudradjat -

    2014-08-01

    Full Text Available Oil palm (Elaeis guineensis Jacq. is one of plantation commodities that contribute to foreign exchange. Palm oil production is influenced by the expansion of the area  and  intensification using the fertilization at main nursery and plantation. Macro elements such as N, P, and K required by the oil palm are in large quantities. The study was conducted from November, 2011 to  May, 2012 at Cikabayan Experimental Station, IPB Darmaga Campus, Bogor. The treatment was laid-out in a factorial randomized block design with three replications. The first factor was P: 0, 3.00, 6.00, and 12.00 g P plant-1 ;  and the second was K: 0, 9.00, 18.00 and 36.00 g K plant-1. The results of the study showed that phosphor did not influence the plant height, number of frond, number of chlorophyll, and stem diameter. Potassium fertilizer influenced the stem diameter at 24 weeks after planting (WAP. There were the interaction between P and K  on stem diameter variable at 16 and 20 WAP.Keywords :  Fertilizer, phosphor, potassium, Elaeis Guineensis Jacq.

  12. Performance of Elaeis Guineensis Leaves Compost in Filter Media for Stormwater Treament Through Column Study

    Takaijudin, H.; Ghani, A. A.; Zakaria, N. A.; Tze, L. L.

    2016-07-01

    Compost based materials arv e widely used in filter media for improving soil capability and plant growth. The aim of this paper is to evaluate different types of compost materials used in engineered soil media through soil column investigation. Three (3) column, namely C1 (control), C2 and C3 had different types compost (10%) which were, commercial compost namely PEATGRO, Compost A and Compost B were prepared with 60% medium sand and 30% of topsoil. The diluted stormwater runoff was flushed to the columns and it was run for six (6) hour experiment. The influent and effluent samples were collected and tested for Water Quality Index (WQI) parameters. The results deduced that C3 with Elaeis Guineensis leaves compost (Compost B) achieved 90.45 (Class II) better than control condition which accomplished 84 (Class II) based on WQI Classification. C3 with Compost A (African Mahogany Leaves Compost) obtained only 59.39 (Class III). C3 with the composition of Compost B effectively removed most pollutants, including Chemical Oxygen Demand (COD, Ammoniacal Nitrogen (NH3-N), were reduced by 89±4% and 96.6±0.9%, respectively. The result concluded that Elaeis Guineensis leaves compost is recommended to be used as part of engineered soil media due to its capabilities in eliminating stormwater pollutants.

  13. Mechanisms Underlying the Endothelium-dependent Vasodilatory Effect of an Aqueous Extract of Elaeis Guineensis Jacq. (Arecaceae) in Porcine Coronary Artery Rings

    Ndiaye, Mamadou; Anselm, Eric; Séne, Madièye; Diatta, Williams; Dièye, Amadou Moctar; Faye, Babacar; Schini-Kerth, Valérie B.

    2009-01-01

    This study was undertaken to investigate the vasodilatory effect of an aqueous extract of Elaeis guineensis Jacq (EGE) in the porcine coronary artery and elicit its possible mechanism(s) of action. Vascular effects of crude extract of dried and powdered leaves of Elaeis guineensis were evaluated on isolated coronary arteries on organ chambers. Determination of eNOS expression and the phosphorylation level of eNOS were determined by Western blot analysis. In the presence of indomethacin, EGE c...

  14. Wound Healing Potential of Elaeis guineensis Jacq Leaves in an Infected Albino Rat Model

    Sreenivasan Sasidharan

    2010-04-01

    Full Text Available Ethnopharmacological relevance:Elaeis guineensisJacq (Arecaceae is one of the plants that are central to the lives of traditional societies in West Africa. It has been reported as a traditional folkloric medicine for a variety of ailments. The plant leaves are also used in some parts of Africa for wound healing, but there are no scientific reports on any wound healing activity of the plant. Aim of the study:To investigate the effects of E. guineensis leaf on wound healing activity in rats. Methods: A phytochemical screening was done to determine the major phytochemicals in the extract. The antimicrobial activity of the extract was examined using the disk diffusion technique and broth dilution method. The wound healing activity of leaves of E. guineensiswas studied by incorporating the methanolic extract in yellow soft paraffin in concentration of 10% (w/w. Wound healing activity was studied by determining the percentage of wound closure, microbial examination of granulated skin tissue and histological analysis in the control and extract treated groups. Results: Phytochemical screening reveals the presence of tannins, alkaloids, steroids, saponins, terpenoids, and flavonoids in the extract. The extract showed significant activity against Candida albicans with an MIC value of 6.25 mg/mL. The results show that the E. guineensis extract has potent wound healing capacity, as evident from better wound closure, improved tissue regeneration at the wound site, and supporting histopathological parameters pertaining to wound healing. Assessment of granulation tissue every fourth day showed a significant reduction in microbial count. Conclusions: E. guineensis accelerated wound healing in rats, thus supporting this traditional use.

  15. Cryopreservation of zygotic embryos and kernels of oil palm (Elaeis guineensis Jacq.)

    Engelmann, Florent; Chabrillange, Nathalie; Dussert, Stéphane; Duval, Yves

    1995-01-01

    Dans cette étude, l'efficacité de deux stratégies pour la cryoconservation du palmier à huile (#Elaeis guineensis$ Jacq.) sont comparées. S'ils sont extraits d'amandes réhydratées, 65% des embryons déshydratés à environ 0,3 g H2O/g MS se développent en plantules après cryoconservation. A l'opposé, seulement 25% des embryons (0,12 g H2O/g MS) extraits d'amandes sèches se développent en plantules. Cependant, cette valeur augmente jusqu'à 63% si les amandes sont partiellement réhydratées avant l...

  16. Characterization of genes related to oil palm (Elaeis guineensis Jacq.) drought stress responses

    Jazayeri, Seyed Mehdi

    2015-01-01

    Palma de aceite (Elaeis guineensis Jacq.) es la primera planta oleaginosa con la producción de más aceite con menos precio. El déficit hídrico reduce la producción de palma de aceite. Es necesario entender las respuestas de plantas al déficit hídrico para obtener plantas más tolerantes. Se usó RNA-Seq para diferenciar dos genotipos de palma de aceite en relación con las respuestas a estrés hídrico. Usando la tecnología de Illumina HiSeq2000 para obtener 1.27 billones de lecturas cortas de 101...

  17. Characterization of Different Parts of Oil Palm Fronds (Elaeis Guineensis and Its Properties

    Noor Afeefah Nordin

    2016-01-01

    Full Text Available The study was conducted on characterizing the properties of oil palm fronds (Elaeis guineensis from different parts. The fronds were cut to three parts which were the top, middle and bottom. The samples were chipped, dried in the oven of 50 °C and ground before they were characterized. The properties of the fronds were characterized using thermal gravimetric analyser (TGA, fourier transform infrared spectroscopy (FTIR and x-ray diffraction (XRD. The results from TGA showed that the bottom part of the frond had the lowest percentage of residues which was 29.22 % and the degradation temperature was 194.17 °C. The FTIR spectra showed almost similar peaks for all samples. The bottom part of the frond had the highest crystallinity index which was 35.26 %. The study proved that different parts of the frond had different characteristics due to its chemical compositions.

  18. Histology of embryoid development in oil palm (Elaeis guineensis Jacq. cell suspension culture

    Songrat Tinnongjig

    2001-11-01

    Full Text Available Embryos of oil palm (Elaeis guineensis Jacq. variety tenera were cultured on Eeuwens or Y3 (1976; 1978 medium supplemented with 2 mg/l 2,4-D. Calluses were initiated from these embryos. The eight-weekold calluses derived from embryos were transferred to modified Y3 liquid medium devoid of 2,4-D and supplemented with NAA, BA and coconut water to establish cell suspension culture. After a period of culture,these cells were then subcultured to the same medium without plant growth regulators to induce embryoid formation. The calluses and embryoids were harvested at various times, fixed, sectioned, stained and examined microscopically. Histological study revealed that embryoid occurred from meristematic cells with dense cytoplasm along the callus clumps.

  19. Detection of potassium deficiency on palm oil tree (Elaeis guineensis (jacq)) by laser induced fluorescence

    The potassium is the main nutrient element which plays a significant role on oil palm tree (Elaeis guineensis (jacq)) production and its resistance to the dry season. One can observe 30% decrease of the production in case of potassium deficiency. The potassium nutrition control of an oil palm tree field is a very important activity and leads to the fertilization policy. The Laser Induced Fluorescence (L.I.F.) is a fast and simple method compared to the classical one, ''Diagnostic Foliaire'', usually used in agronomy. We used the L.I.F. method to detect the oil palm tree stress caused by potassium deficiency, analysing the fluorescence spectrum of the chlorophyll a. We proved that the intensity ratio of the fluorescence spectrum R=F690/F73S is superior to 0.5 when the tree is under stress and its value is around 0.4 in case of intact tree. (author)

  20. Gamma irradiations of the seeds and pollen grains of oil palm (Elaeis guineensis Jacq.)

    The pollen grains and seeds of oil palm (Elaeis guineensis Jacq.) were irradiated with up to 1400 Gy of gamma rays from a 60Co source. Pollen viability tests and subsequent development of embryos from the irradiated seeds showed that a dosage of up to 50 Gy could be tolerated by both the pollen grains and the seeds. Higher doses caused a drastic drop in the germination of seeds, thus reducing the potential size of any population for further studies. Plants of high bunch production were identified in both irradiated and non-irradiated materials. The potential use of gamma irradiation to induce useful mutation in the characters contributing to yield in the oil palm is discussed. (author). 2 refs, 3 figs, 1 tab

  1. Pertumbuhan Eksplan Bunga Betina Kelapa Sawit (Elaeis guineensis Jacq.) Pada Media MS Dengan Kombinasi 2,4D Dan BAP

    Nasution, Siti Shofiya

    2015-01-01

    Penelitian tentang “Pertumbuhan Eksplan Bunga Betina Kelapa Sawit (Elaeis guineensis Jacq.) pada Media MS dengan Kombinasi 2,4-D dan BAP” telah dilakukan di Laboratorium Kultur Jaringan Tumbuhan Departemen Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara dari bulan Januari 2013 sampai dengan bulan Desember 2013. Penelitian yang bertujuan untuk mengetahui kombinasi 2,4-D dan BAP terbaik pada induksi kalus dan pertumbuhan kultur eksplan bunga betina kelapa sawit...

  2. Feeding oil palm (Elaeis guineensis, Jacq.) fronds alters rumen protozoal population and ruminal fermentation pattern in goats

    Mahdi Ebrahimi; Rajion, Mohamed A; Goh Y. Meng; Parisa Shokryazdan; Awis Qurni Sazili; Mohammad Faseleh Jahromi

    2015-01-01

    Oil palm fronds (OPF), normally available all the year round, may provide a sustainable ruminant feed for livestock industry in tropical regions. A feeding trial was conducted to study the effects of feeding OPF on the rumen protozoal population, rumen fermentation and fatty acid profiles of rumen fluid in goats. Twentyfour five-month-old Kacang crossbred male goats were individually housed and fed for 100 d with concentrate diets supplemented with oil palm (Elaeis guineensis Jacq.) frond pel...

  3. Peningkatan Kualitas Kayu Kelapa Sawit (Elaeis guineensis Jacq.) Yang Di Kompregnasi Dengan Resin Getah Damar (Agathis dammara)

    Widiarti, Leni

    2015-01-01

    The quality increasing of oil palm trunk (Elaeis guineensis Jacq.) by compregnated with dammar resin (Agathis dammara) has been performed. Sellulose as a natural polymer of the oil palm trunk interacted with dammar resin and the mechanical properties of the oil palm trunk was increased. OPT compregnate with dammar resin was performed in vacum compregnator with the duration time of two seconds. The temperature of compregnate was room temperature with 0%, 10%, 20%, 30%, and 40% concentrations o...

  4. Pertumbuhan Bibit Kelapa Sawit ( Elaeis Guineensis Jacq.) Dengan Menggunakan Media Sekam Padi dan Frekuensi Penyiraman di Main Nursery

    Hermanto

    2014-01-01

    HERMANTO : The Growth of Oil Palm Seedlings (Elaeis guineensis Jacq.) by the application of Rice Husk And Frequency Of Watering In The Main Nursery. Supervised by FERRY AZRA T SITEPU and JONATAN GINTING. This research aims to study aimed to determine the growth of oil palm seedlings to rice husk and watering frequency on the main nursery. This research was conducted in Greenhouses in the Faculty of Agriculture, University of North Sumatera, Medan with ± 25 m altitude above sea level in J...

  5. Studi Analisis Residu Klorpirifos dalam Minyak Sawit (Elaeis Guineensis Jacq) Menggunakan Kromatografi Gas dengan Detektor Penangkap Elektron

    Sugianto, Yogi

    2010-01-01

    A research has been done about the residue of chlorpyrifos, a substance which was used as active content in pesticide, in coconut palm oil (Elaeis guineensis Jacq). The extensive usage of chlorpyrifos pesticide in the field of farming and plantation leaves residue of chlorpyrifos in the farming and plantation product. The danger of chlorpyrifos residue presence is very fatal. It can lead to death. Chlorpyrifos (a pesticide containing phosphorus and chlorine) was determined by using gas c...

  6. Phenology of the oil palm interspecific hybrid Elaeis oleifera × Elaeis guineensis

    Paola Hormaza

    2012-01-01

    Full Text Available Oil palm is one of the most important oil crops in the world. Because of its high productivity and perennial nature, it has been expanding quickly. Commercial plantations consist mostly of the African palm E. guineensis Jacq. However, producers in Latin America are increasingly planting the O × G interspecific hybrid, a cross between African palm (E. guineensis and the American palm (E. oleifera (Kunth Cortés. This interspecific hybrid has emerged as a promising solution to diseases such as the bud rot of oil palm because of the apparent partial resistance of this genotype to the disease. This work studied and described the phenology of the O × G interspecific hybrid. The phenology stages were coded using the BBCH scale. The scale for the phenophases was defined using a three-digit code. Due to the nature of the palm, no descriptions were used for stage two (formation of side shoots/tillering and stage four (development of harvestable vegetative plant parts or vegetative reproductive organs because these stages do not apply to oil palm. The scale was constructed using germinating seeds, pre-nursery and nursery plants and five year-old palms. For the description of the stem elongation, different age palms of the same O × G hybrid were used. Observations were performed during an 18-month period. Additionally, the interval for the change from one phenology stage to another was determined both in days and degree-days (DD. The interspecific O × G hybrid required 6408 DD from when the spear leaf unfolds until the bunch was ripened and harvested, and 4427.6 DD from leaf unfolding to anthesis.

  7. Estimación del área y del peso seco foliar en elaeis guineellsis, elaeis oleifera y el hibrido interespecifico e. guineensis x e. oleífera

    Contreras, Angela P.; Corchuelo, German; Martinez, Orlando; Cayon, Gerardo

    2011-01-01

    Este trabajo se planteo can la finalidad de determinar modelos estadísticos que permitan estimar el área y el peso foliar a través de métodos indirectos (no destructivos) en Elaeis guineensis (Palma africana), Elaeis oleifera (Palma Noli), y el hibrido interespecifico E. guineensis X E. oleifera (Afrieana X Noli ). Los experimentos de campo se efectuaron en las Haciendas Santa Bárbara y Chaparral-Cuernavaca, de la plantación Unipalma, ubicadas en la zona palmera de los llanos orientales en Co...

  8. In vitro anti-plasmodial activity of three herbal remedies for malaria in Ghana: Adenia cissampeloides (Planch.) Harms., Termina liaivorensis A. Chev, and Elaeis guineensis Jacq

    Kofi Annan; K Sarpong; Asare, C.; Dickson, R.; K I Amponsah; Gyan, B.; Ofori, M.; Gbedema, S Y

    2012-01-01

    Background: Herbal remedies of Adenia cissampeloides, Terminalia ivorensis, and Elaeis guineensis among others have been used in Ghana for the treatment of various ailments including malaria. However, most of these remedies have not been scientifically investigated. Objective: This study, therefore, seeks to investigate the anti-plasmodial activity of these plants. Materials and Methods: The ethanolic extracts of A. cissampeloides stem, T. ivorensis stem bark, and E. guineensis leaves were te...

  9. Adoption of the Tenera Hybrid of Oil Palm (Elaeis guineensis Jacquin. among Smallholder Farmers in Cameroon

    Assoumou Mezui, MR.

    2013-01-01

    Full Text Available The adoption of the Tenera oil palm (Elaeis guineensis Jacq. type was studied by focusing on 208 family farmers in Cameroon. The "Average Treatment Effect" (ATE method was used. This method enables to estimate, by use of a Probit model, the effects created when a farmer is exposed to treatment (or intervention, which represents a source of improved oil palm planting material. According to the results, the estimated adoption rate for Tenera is highly significant (P< 0.05 for all the categories of producers, regardless of the supply source, which may be formal or informal. In addition, the main factors that significantly determine this adoption are: availability of arable land in forested areas and whether growers intend to increase the size of their plantations. However, the supply of pure planting material remains an important constraint because the other two types of oil palm (Dura and Pisifera which produce poor palm oil yields are still mistakenly planted on many family farms in Cameroon. Further studies will address this issue in the aim of identifying appropriate solutions.

  10. Pollen viability and its effect on fruit set of oil palm (Elaeis guineensis Jacq.

    ALFIN WIDIASTUTI

    2008-01-01

    Full Text Available The research was aimed at studying (1 the decline of pollen viability during storage, and (2 the effect of pollen amount on fruit set of oil palm (Elaeis guineensis Jacq.. The experiment was conducted at PT. Dami Mas Sejahtera and PT. Sinar Mas Agro Resource and Technology (SMART Tbk, Riau from February to August 2004. The first experiment was investigated up to six months storage period in the refrigerator, whereas in the second experiment a randomized complete block design with two factors was used: length of storage, i.e. 0, 1 and 2 months and amount of pollen, i.e. 0.022, 0.044, 0.066, 0.088, and 0.11 g mixed with powder to 10g to pollinate an inflorescence. The result showed that the viability of pollen started to decline three months after storage from about 92% to 83%, and declined to about 75% after six months of storage. Result of the second experiment showed that storage of pollen up to two months did not affect percentage of normal fruit, although the percentage of parthenocarpic fruits was decreased. This could be due to the high viability of pollen as the viability was remained high (about 90% after being stored for two months in the refrigerator. Pollen with high viability could be used in a smaller amount to pollinate a female inflorescence without affecting fruit set of about 70-76%.SD037 had a higher reproductive success than SD038 and SD39.

  11. Genetic variation and phylogenetic relationships in oil palm (Elaeis guineensis Jacq. based on RAPD analysis

    Nualsri, C.

    2005-05-01

    Full Text Available The genetic variability and phylogenetic relationships in oil palm (Elaeis guineensis Jacq. were studied using RAPD (Random Amplified Polymorphic DNA. Leaf samples of 151 plants were collected from different areas in southern Thailand. DNA from the leaf samples was isolated using CTAB buffer and screened by decamer oligonucleotide primers. Among the total of 160 primers screened, 7 primers (OPB-08, OPR-11, OPT-06, OPT-19, OPAB-01, OPAB-09 and OPAB-14 were chosen to analyse for genetic variation in 151 individuals representing 52 dura, 60 tenera and 39 pisifera. Two hundred and nine amplified fragments were obtained from 7 primers with an average of 29.85 RAPD markers per primer. A dendrogram showing genetic similarities among oil palm was constructed based on polymorphic bands using UPGMA (Unweighted Pair-Group Method Using Arithmetic Average. Cluster analysis was performed using the SPSS program, which revealed four major clusters: 1 dura, tenera and pisifera from Paorong Oil Palm Company, Oil Palm Research Center, dura and tenera from private plantation in Krabi, and dura from Thepa Research Station;2 dura and tenera from Thai Boonthong Company, pisifera and tenera from Thepa Research Station, dura, tenera and pisifera from Klong Hoi Khong Research Station; 3 and 4 dura and tenera from Univanit Company, respectively. In general, a similarity index showed relatively high levels of 0.6 or greater.

  12. Soil Compaction and Oil Palm (Elaeis guineensis Yield in a Clay Textured Soil

    Zuraidah Yahya

    2010-01-01

    Full Text Available Problem statement: The impacts of soil compaction on crop yields have been studied extensively by soil scientists due to declining soil productivity associated with mechanisation. However, a relationship between machine-induced soil compaction and oil palm (Elaeis guineensis yield is unclear. Therefore, the objectives of this study were to determine the effects of mechanization on soil physical properties and the influence on oil palm yield. Approach: The palms were planted in Bernam series soil which is clay textured. Compaction treatments were imposed for 6 consecutive years. Comparisons were made between the effects of soil compaction caused by different trailer weights and monthly transportation frequency. Results: The results showed a beneficial effect of soil compaction on the oil palm yield. It significantly increased the yield with increased mean soil bulk density. The transportation frequency played a greater role than the trailer weight. After six years of soil compaction, there was a positive relationship between mean soil bulk density, porosity and oil palm yield. Conclusion: Thus compaction may not often be a problem.

  13. Comparative Proteome Analysis of Oil Palm (Elaeis guineensis Jacq.) Floret and Fruit Tissues

    Kok S.Y.; Ong-Abdullah M.

    2012-01-01

    Marked changes are expected when flowers are fertilized and fruit formation is initiated.In this study,differentially expressed proteins between florets (day before pollination and day after pollination) and fruit (2 weeks after pollination) tissues of oil palm (Elaeis guineensis Jacq.) were identified and characterized.Proteins were extracted using phenol-based method and subjected to one-and two-dimensional gel electrophoresis.One-dimensional gel electrophoresis of protein extracts showed a number of protein bands common to both types of floret and fruit tissues.Subsequently,protein extracts resolved by two-dimensional gel electrophoresis in the 4 to 7 pH range revealed about 53 expressed proteins showed significant differences during fertilization and fruit formation.The differentially expressed proteins were excised and digested with trypsin.Proteins were then identified with the matrix-assisted laser desorption ionization-time of flight tandem mass spectrometry (MALDI-TOF/TOF-MS),resulting in a 73.5% success in protein identification.The identified proteins were mainly involved in protein metabolism,biological regulation,stress response and oxidationreduction process.Further verification of the results are underway but nonetheless this study has provided some valuable information on fertilization and early fruit formation in oil palm.

  14. Collection of Oil Palm (Elaeis guineensis Jacq. Germplasm in the Northern Regions of Ghana

    E. Sapey

    2012-09-01

    Full Text Available Oil palm germplasm collection was carried out in the Northern Regions of Ghana for evaluation, screening for drought tolerance and further incorporation into breeding programmes of Ghana’s Council for Scientific and Industrial Research (C.S.I.R-Oil Palm Research Institute (O.P.R.I. The study highlights the collection of 22 oil palm (Elaeis guineensis accessions from 5 locations in the Northern Regions of Ghana. The Northern Regions are not suitable for oil palm cultivation due to unfavourable weather conditions. Only Dura (D forms of Oil palm were present without the existence of the other forms. The smallest shell thickness recorded was 1.6 mm. Data on stem height, bunch weight, bunch length, bunch depth, bunch diameter, bunch width, bunch spine length, bunch stalk weight, fruit length and width were recorded in-situ. Mesocarp, kernel and shell to fruit ratios were computed. Data collected were analysed using standard procedures; Elementary statistics (mean values, standard error, range and co-efficient of variation were computed. Results revealed some variation for both qualitative and quantitative traits assessed on the accessions. Seedlings have been raised from these accessions for testing for drought screening and yield evaluation.

  15. Spatial distribution of Metamasius hemipterus (Coleoptera: Curculionidae) in an oil palm plantation (Elaeis guineensis Jacq) in Roraima = Distribuição espacial de Metamasius hemipterus (Coleoptera: Curculionidae) em plantio de dendê (Elaeis guineensis Jacq) em Roraima

    Luiz Fernandes Silva Dionisio; Antonio Cesar Silva Lima; Elisangela Gomes Fidelis de Morais; Ruy Guilherme Correia; Artur Vinícius Ferreira dos Santos; Carla Klis dos Santos Ximenes

    2015-01-01

    The aim of this study was to evaluate the spatial distribution pattern and aggregation radius of Metamasius hemipterus in an oil palm (Elaeis guineensis) plantation, in the state of Roraima, Brazil (RR). The experimental area is located in the town of São João da Baliza, RR, where 24 samples were taken from September 2013 to August 2014. The perimeter of the area was marked out using the global positioning system, with sampling grids of 45 x 45 m. Spatial distribution was determined by pl...

  16. Spatial distribution of Metamasius hemipterus (Coleoptera: Curculionidae) in planting oil palm (Elaeis guineensis Jacq) in Roraima = Distribuição espacial de Metamasius hemipterus (Coleoptera: Curculionidae) em plantio de dendê (Elaeis guineensis Jacq) em Roraima

    Antonio Cesar Silva Lima; Elisangela Gomes Fidelis de Morais; Ruy Guilherme Correia; Artur Vinícius Ferreira dos Santos; Carla Klis dos Santos Ximenes

    2015-01-01

    The aim of this study was to evaluate the spatial distribution pattern and aggregation radius of Metamasius hemipterus in an oil palm (Elaeis guineensis) plantation, in the state of Roraima, Brazil (RR). The experimental area is located in the town of São João da Baliza, RR, where 24 samples were taken from September 2013 to August 2014. The perimeter of the area was marked out using the global positioning system, with sampling grids of 45 x 45 m. Spatial distribution was determined by placin...

  17. Estructura genética de poblaciones naturales de palma de aceite (Elaeis guineensis Jacq.) procedentes de Angola

    Barrera Galvis, Sandra Nereida

    2011-01-01

    El objetivo del presente estudio fue evaluar la diversidad y estructura genética de una colección de 455 entradas de palma africana (Elaeis guineensis Jacq) colectadas en 5 zonas geográficas de la República de Angola y 9 híbridos interespecíficos, con 19 loci microsatélite. Un total de 79 alelos fueron detectados con un promedio de 5.61, trece loci fueron polimórficos con un PIC (índice de información polimórfica) promedio de 0,533. La heterocigosidad esperada promedio (0,561) mostro un...

  18. Pengaruh Penambahan Nanokristal Selulosa Dari Tandan Kosong Kelapa Sawit (Elaeis Guineensis Jack) Terhadap Produk Karet Nanokomposit Dengan Teknik Pencelupan

    Yuliana, Yesti

    2015-01-01

    Research on the effect of improving film nanocrystal cellulose from palm empty fruit bunches (Elaeis Guineensis Jack) for making nanocomposite rubber with dipping technique have been done. This research was carried out in three steps : i.e isolation of α-cellulose from palm empty fruit bunches, isolation of nanocrystal cellulose from α-cellulose wih hydrolysis process using H₂SO₄ 48,84 % at 45˚C for 25 minutes, nanocomposite rubber preparation processes. Functional groups analysis of α-cellu...

  19. Optimasi Berat Natrium Monokloroasetat dan Waktu Sintesis Karboksimetil Selulosa (CMC) Dari Tandan Kosong Kelapa Sawit (Elaeis guineensis jack)

    Martinni, Martha

    2016-01-01

    Has done research on weight optimization and synthesis time monokloroasetat sodium carboxymethyl cellulose (CMC) from empty fruit bunches of oil palm (Elaeis guineensis jack). This study was conducted in two phases, namely the isolation of α-cellulose powder empty fruit bunches of oil palm, and the process of synthesis of cellulose with the addition of α-cellulose using NaOH 40%, and sodium monokloroasetat the weight variation 5, 6, 7, 8, and 9 g at a temperature of 60 °C and glacial acetic a...

  20. Effect of Preservation Methods of Oil Palm Sap (Elaeis guineensis) on the Reproductive Indices of Male Wistar Rats

    Ikegwu, Theophilus Maduabuchukwu; Okafor, Gabriel Ifeanyi; Ochiogu, Izuchukwu Shedrack

    2014-01-01

    Thirty male Wistar rats, split into five groups of six rats each, were administered different forms of oil palm tree (Elaeis guineensis) sap samples by gavage based on 1.5% of their weekly body weights. Group 1 which served as control received only water, group 2 received pasteurized palm sap (PPS), group 3 received market palm wine (MPW), group 4 received frozen palm sap (FPS), whereas group 5 received fresh palm sap (FrPS). Chemical composition of the sap samples was determined. Normal feed...

  1. Isolation, expression analysis and characterization of EgNDL, a NDR-like protein in oil palm (Elaeis guineensis Jacq.)

    Kedsirin Ruttajorn; Alisa Nakkaew; Tanate Panrat; Arunrut Vanichanon; Amornrat Phongdara

    2016-01-01

    A novel cDNA of a SF21-like protein or NDR-like protein (EgNDL) from Elaeis guineensis Jacq. is 1,044 bp in length and encodes a putative protein with a 347-amino-acid open reading frame. The EgNDL showed 93% identity to the pollenspecific SF21-like protein of Phoenix dactylifera and also showed 79% identity to the NDL protein of Theobroma cacao. Expression analysis of the EgNDL gene in various tissues showed that EgNDL was expressed in the anthers, pistils, mesocarp and leaves. T...

  2. ASSESSMENT OF NIGERIAN WILD OIL PALM (ELAEIS GUINEENSIS JACQ. POPULATIONS IN CROSSES WITH DELI TESTERS

    Noumouha E. N. Ghislain

    2014-08-01

    Full Text Available To widen the genetic variability and especially to enrich agronomic qualities of populations of oil palm (Elaeis guineensis Jacq. of Group B used in the breeding scheme, a study was led on four populations (Abak, Ahoada, Ayangba and Uli prospected in Nigeria. Sixteen palms chosen in these populations were evaluated in crosses with 10 Deli testers derived from the second cycle of reciprocal recurrent selection scheme. The LM 2 T × DA 10 D and LM 2 T × DA 115 D progenies derived from the first breeding cycle were used as controls. The bunch production components, vascular wilt susceptibility and vertical growth rate were used as criterion of evaluation. The Abak × Deli, Ahoada × Deli and Uli × Deli materials were characterized at the young age by bunch productions higher than that of the LM 2 T × DA 10 D control. The bunch productions at the adult period of various Nigerian × Deli materials were lower than those of the two controls. Abak × Deli material was tolerant to the vascular wilt. Ayangba × Deli material had a slower vertical growth rate than those of the two controls. Seven parents were identified in the four populations for their good general combining ability for the precocity and/or vascular wilt tolerance or the slow vertical growth rate. Prospects for utilization of these parents for the improvement of bunch production at the young age, the reduction of the vertical growth and the diversification of sources of vascular wilt tolerance of populations used in the reciprocal recurrent selection scheme were discussed.

  3. RNA-Seq Analysis of Oil Palm under Cold Stress Reveals a Different C-Repeat Binding Factor (CBF) Mediated Gene Expression Pattern in Elaeis guineensis Compared to Other Species

    Xintao Lei; Yong Xiao; Wei Xia; Mason, Annaliese S.; Yaodong Yang; Zilong Ma; Ming Peng

    2014-01-01

    Elaeis guineensis as a tropical oil-crop is particularly sensitive to low temperature. Improvement of cold-tolerance may significantly increase the total cultivation area of this tropical oil-crop worldwide. We sequenced cold-treated and control (untreated) samples of Elaeis guineensis. De novo assembly generated 51,452 unigenes with an average length of 703 bp. Subsequently, these expressed sequences were functionally annotated. In the K category (transcription factors) of COG (Cluster of Or...

  4. Effects of the Fruit Ripening Stage on Antioxidant Capacity, Total Phenolics, and Polyphenolic Composition of Crude Palm Oil from Interspecific Hybrid Elaeis oleifera × Elaeis guineensis.

    Rodríguez, Juanita C; Gómez, Daniela; Pacetti, Deborah; Núñez, Oscar; Gagliardi, Riccardo; Frega, Natale G; Ojeda, Myriam L; Loizzo, Monica R; Tundis, Rosa; Lucci, Paolo

    2016-02-01

    In the present study, we assessed for the first time the changes in the antioxidant capacity, total phenolic content, and polyphenolic composition of interspecific hybrid palm oil extracted from Elaeis oleifera × Elaeis guineensis (O × G, Coari × La Mé cultivar) during the fruit ripening process 18, 20, 22, and 24 weeks after anthesis. A progressive decrease (p < 0.05) of phenolic content occurred during fruit development together with marked changes in polyphenol profiles. Significant negative correlations were established between antioxidant activity measured by TEAC (R = -0.954; p < 0.05) and ORAC (R = -0.745; p < 0.05) and the fruit ripening stage, while a positive correlation between total phenolic content was found using either the TEAC assay or the ORAC assay. The highest DPPH radical scavenging activity was also obtained with oils extracted at 18 WAA. These results highlight that O × G fruits of early ripeness represent a better source of phenolic compounds and may provide extracts with higher antioxidant activities when hybrid palm oil is aimed to be used as a functional ingredient for the development of food or food products with antioxidant properties. PMID:26752619

  5. Ambient Aerosol in Southeast Asia: High Resolution Aerosol Mass Spectrometer Measurements Over Oil Palm (Elaeis guineensis)

    Phillips, G.; Dimarco, C.; Misztal, P.; Nemitz, E.; Farmer, D.; Kimmel, J.; Jimenez, J.

    2008-12-01

    The emission of organic compounds in the troposphere is important factor in the formation of secondary organic aerosol (SOA). A very large proportion of organic material emitted globally is estimated to arise from biogenic sources, with almost half coming from tropical and sub-tropical forests. Preliminary analyses of leave cuvette emission studies suggest that oil palm (Elaeis guineensis) is a significantly larger source of isoprene than tropical forest. Much larger sources of isoprene over oil palm allied with a larger anthropogenic component of local emissions contrast greatly with the remote tropical forest environment and therefore the character of SOA formed may differ significantly. These issues, allied with the high price of palm oil on international markets leading to increased use of land for oil palm production, could give rise to rapidly changing chemical and aerosol regimes in the tropics. It is therefore important to understand the current emissions and composition of organic aerosol over all important land-uses in the tropical environment. This in turn will lead to a greater understanding of the present, and to an improvement in predictive capacity for the future system. To help address these issues, a high resolution time of flight aerosol mass spectrometer (HR-ToF-AMS) was deployed in the Sabahmas (PPB OIL) oil palm plantation near Lahad Datu, in Eastern Sabah, as part of the field component of the Aerosol Coupling in the Earth System (ACES) project, part of the UK NERC APPRAISE program. This project was allied closely with measurements made of similar chemical species and aerosol components at a forest site in the Danum Valley as part of the UK Oxidant and Particle Photochemical Processes above a Southeast Asian tropical rainforest (OP3) project. Measurements of submicron non- refractory aerosol composition are presented along with some preliminary analysis of chemically resolved aerosol fluxes made with a new eddy covariance system, based on the

  6. Application of Response Surface Methodology in Extraction of Bioactive Component from Palm Leaves (Elaeis guineensis

    Nur Afiqah Arham

    2013-10-01

    Full Text Available Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 The hydroxyl groups of the polyphenols are capable to act as reducing agent for reduction reaction. The effect of drying temperature, extraction temperature and extraction duration were evaluated using central composite design which consists of 20 experimental runs. Response surface methodology (RSM was used to estimate the optimum parameters in extracting polyphenols from the palm leaves. The correspondence analysis of the results yielded a quadratic model which can be used to find optimum conditions of extraction process. The optimum extraction condition of drying temperature, extraction temperature and extraction duration are 70°C, at 70°C of 10 minutes, respectively. Total polyphenols were determined by application of the Folin-Ciocalteu micro method and the extract was found contain of 8 mg GAE/g dry palm leaves at optimum conditions. Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Doi: 10.12777/ijse.5.2.95-100 [How to cite this article: Arham, N.A., Mohamad, N.A.N., Jai, J., Krishnan, J., Noorsuhana Mohd Yusof, N.M. (2013. Application of Response Surface Methodology in Extraction of Bioactive Component from Palm Leaves (Elaeis guineensis. International Journal of Science and

  7. Oil palm (Elaeis guineensis Jacq. tissue culture ESTs: Identifying genes associated with callogenesis and embryogenesis

    Ooi Leslie CL

    2008-05-01

    Full Text Available Abstract Background Oil palm (Elaeis guineensis Jacq. is one of the most important oil bearing crops in the world. However, genetic improvement of oil palm through conventional breeding is extremely slow and costly, as the breeding cycle can take up to 10 years. This has brought about interest in vegetative propagation of oil palm. Since the introduction of oil palm tissue culture in the 1970s, clonal propagation has proven to be useful, not only in producing uniform planting materials, but also in the development of the genetic engineering programme. Despite considerable progress in improving the tissue culture techniques, the callusing and embryogenesis rates from proliferating callus cultures remain very low. Thus, understanding the gene diversity and expression profiles in oil palm tissue culture is critical in increasing the efficiency of these processes. Results A total of 12 standard cDNA libraries, representing three main developmental stages in oil palm tissue culture, were generated in this study. Random sequencing of clones from these cDNA libraries generated 17,599 expressed sequence tags (ESTs. The ESTs were analysed, annotated and assembled to generate 9,584 putative unigenes distributed in 3,268 consensi and 6,316 singletons. These unigenes were assigned putative functions based on similarity and gene ontology annotations. Cluster analysis, which surveyed the relatedness of each library based on the abundance of ESTs in each consensus, revealed that lipid transfer proteins were highly expressed in embryogenic tissues. A glutathione S-transferase was found to be highly expressed in non-embryogenic callus. Further analysis of the unigenes identified 648 non-redundant simple sequence repeats and 211 putative full-length open reading frames. Conclusion This study has provided an overview of genes expressed during oil palm tissue culture. Candidate genes with expression that are modulated during tissue culture were identified. However

  8. Estimación del área y del peso seco foliar en Elaeis guineellsis, Elaeis oleifera y el hibrido interespecifico E. Guineensis x E. Oleífera

    Contreras Angela P.; Corchuelo German; Martinez Orlando; Cayon Gerardo

    1999-01-01

    Este trabajo se planteo can la finalidad de determinar modelos estadísticos que permitan estimar el área y el peso foliar a través de métodos indirectos (no destructivos) en Elaeis guineensis (Palma africana), Elaeis oleifera (...

  9. Molecular Characterization of the Elaeis guineensis Medium-Chain Fatty Acid Diacylglycerol Acyltransferase DGAT1-1 by Heterologous Expression in Yarrowia lipolytica.

    Aymé, Laure; Jolivet, Pascale; Nicaud, Jean-Marc; Chardot, Thierry

    2015-01-01

    Diacylglycerol acyltransferases (DGAT) are involved in the acylation of sn-1,2-diacylglycerol. Palm kernel oil, extracted from Elaeis guineensis (oil palm) seeds, has a high content of medium-chain fatty acids mainly lauric acid (C12:0). A putative E. guineensis diacylglycerol acyltransferase gene (EgDGAT1-1) is expressed at the onset of lauric acid accumulation in the seed endosperm suggesting that it is a determinant of medium-chain triacylglycerol storage. To test this hypothesis, we thoro...

  10. Molecular Characterization of the Elaeis guineensis Medium-Chain Fatty Acid Diacylglycerol Acyltransferase DGAT1-1 by Heterologous Expression in Yarrowia lipolytica

    Aymé, Laure; Jolivet, Pascale; Nicaud, Jean-Marc; Chardot, Thierry

    2015-01-01

    Diacylglycerol acyltransferases (DGAT) are involved in the acylation of sn-1,2-diacylglycerol. Palm kernel oil, extracted from Elaeis guineensis (oil palm) seeds, has a high content of medium-chain fatty acids mainly lauric acid (C12:0). A putative E. guineensis diacylglycerol acyltransferase gene (EgDGAT1-1) is expressed at the onset of lauric acid accumulation in the seed endosperm suggesting that it is a determinant of medium-chain triacylglycerol storage. To test this hypothesis, we thoro...

  11. Isolation and characterization of cellulose nanocrystals from parenchyma and vascular bundle of oil palm trunk (Elaeis guineensis).

    Lamaming, Junidah; Hashim, Rokiah; Leh, Cheu Peng; Sulaiman, Othman; Sugimoto, Tomoko; Nasir, Mohammed

    2015-12-10

    In this study cellulose nanocrystals were isolated through acid hydrolysis process from parenchyma and vascular bundle of oil palm trunk (Elaeis guineensis). The morphological properties of obtained cellulose nanocrystals were studied by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The microscopy images showed smoother and cleaner surface of parenchyma cellulose nanocrystals when compared to vascular bundle cellulose nanocrystals. The TEM image shows a higher length and diameter for parenchyma cellulose nanocrystals compared to vascular bundle cellulose nanocrystals. The Fourier transform infrared (FTIR) spectra showed changes in functional groups after acid hydrolysis due to removal of lignin, hemicelluloses and other impurities in both type of cellulose nanocrystals. Crystallinity index of cellulose nanocrystals was observed higher for vascular bundle as compared to parenchyma. Thermogravimetric analysis (TGA) was performed to study the thermal stability of cellulose nanocrystals and it was observed higher for parenchyma cellulose nanocrystals compared to vascular bundle. PMID:26428155

  12. SSR markers in transcripts of genes linked to post-transcriptional and transcriptional regulatory functions during vegetative and reproductive development of Elaeis guineensis

    Tranbarger Timothy; Kluabmongkol Wanwisa; Sangsrakru Duangjai; Morcillo Fabienne; Tregear James W; Tragoonrung Somvong; Billotte Norbert

    2012-01-01

    Abstract Background The oil palm (Elaeis guineensis Jacq.) is a perennial monocotyledonous tropical crop species that is now the world's number one source of edible vegetable oil, and the richest dietary source of provitamin A. While new elite genotypes from traditional breeding programs provide steady yield increases, the long selection cycle (10-12 years) and the large areas required to cultivate oil palm make genetic improvement slow and labor intensive. Molecular breeding programs have th...

  13. Optimization of media constituents for the production of lipase in solid state fermentation by Yarrowia lipolytica from palm Kernal cake (Elaeis guineensis)

    Sarat Babu Imandi; Sita Kumari Karanam; Hanumantha Rao Garapati

    2010-01-01

    The production of extra cellular lipase in Solid State Fermentation (SSF) using Yarrowia lipolytica NCIM 3589 with Palm Kernal cake (Elaeis guineensis) has been studied. Different parameters such as incubation time, inoculum level, initial moisture content, carbon level and nitrogen level of the medium were optimized. Screening of various process variables has been accomplished with the help of Plackett-Burman design. The maximum lipase activity of 18.58 units per gram of dry fermented substr...

  14. Chronic Administration of Oil Palm (Elaeis guineensis) Leaves Extract Attenuates Hyperglycaemic-Induced Oxidative Stress and Improves Renal Histopathology and Function in Experimental Diabetes

    Varatharajan Rajavel; Munavvar Zubaid Abdul Sattar; Mahmood Ameen Abdulla; Kassim, Normadiah M.; Nor Azizan Abdullah

    2012-01-01

    Oil palm (Elaeis guineensis) leaves extract (OPLE) has antioxidant properties and because oxidative stress contributes to the pathogenesis of diabetic nephropathy (DN), we tested the hypothesis that OPLE prevents diabetes renal oxidative stress, attenuating injury. Sprague-Dawley rats received OPLE (200 and 500 mg kg−1) for 4 and 12 weeks after diabetes induction (streptozotocin 60 mg kg−1). Blood glucose level, body and kidney weights, urine flow rate (UFR), glomerular filtration rate (GFR),...

  15. The development and application of molecular markers for linkage mapping and quantitative trait loci analysis of important agronomic traits in oil palm (Elaeis guineensis Jacq.)

    Gan, Siou Ting

    2014-01-01

    Oil palm (Elaeis guineensis) produces over five times more oil/year/hectare than oil seed rape and accounted for 33% of world vegetable oil production in 2011. Being a cross-pollinated perennial tree crop with long breeding cycles (typically 12 years) and a large planting area requirement (usually 143 palms/hectare), utilization of molecular technology could greatly improve the efficiency of oil palm breeding. In the present study, various approaches were used to develop molecular markers for...

  16. Model pendugaan cadangan karbon pada kelapa sawit (Elaeis guineensis Jacq.) umur 5 tahun di perkebunan kelapa sawit PT. Putri Hijau, Kabupaten Langkat.

    Silaban, Dedy Hamonangan

    2015-01-01

    Palm Oil Plant a CO2 absorber as well as other crops such as forest plants. Currentlythe center ofoil palm plantationslocatedin the province ofNorth Sumatra. This study aimedtoobtaincarbonestimationmodelsAllometricpalmplantationsinPT. PutriHijau, Langkatand to getpotentialcarbon contentof plantoil palm(Elaeis guineensisJacq.) Age5years. The researchcarried outin twostages, the first stage was datacollectioninthe field andthe secondstage wasbiomassandcarbonanalyzesof each ofthe laboratory. The...

  17. Pengaruh Lama Fermentasi Dan Berat Ragi Roti Terhadap Kadar Bioetanol Dari Fermentasi Glukosa Hasil Hidrolisis Selulosa Tandan Kosong Kelapa Sawit (Elaeis Guineensis Jack) Dengan HCl 30%

    Suri, Annisa

    2013-01-01

    The research about the effect of fermentation time and weight of yeast bread on bioethanol concentration from fermentation the glucose from hydrolysis of cellulose oil palmempty fruit bunches (Elaeis guineensis Jack ) with HCl 30% has done. From the research, it found that oil palm empty fruit bunches containing cellulose of 24.1298 %. The cellulose was isolated from oil palm empty fruit bunches. It was hidrolized by HCl 30% to yield glucose and was analized by Nelson-Somogyi Method and the ...

  18. Genetic architecture of palm oil fatty acid composition in cultivated oil palm (Elaeis guineensis Jacq. compared to its wild relative E. oleifera (H.B.K Cortes.

    Carmenza Montoya

    Full Text Available We searched for quantitative trait loci (QTL associated with the palm oil fatty acid composition of mature fruits of the oil palm E. guineensis Jacq. in comparison with its wild relative E. oleifera (H.B.K Cortés. The oil palm cross LM2T x DA10D between two heterozygous parents was considered in our experiment as an intraspecific representative of E. guineensis. Its QTLs were compared to QTLs published for the same traits in an interspecific Elaeis pseudo-backcross used as an indirect representative of E. oleifera. Few correlations were found in E. guineensis between pulp fatty acid proportions and yield traits, allowing for the rather independent selection of both types of traits. Sixteen QTLs affecting palm oil fatty acid proportions and iodine value were identified in oil palm. The phenotypic variation explained by the detected QTLs was low to medium in E. guineensis, ranging between 10% and 36%. The explained cumulative variation was 29% for palmitic acid C16:0 (one QTL, 68% for stearic acid C18:0 (two QTLs, 50% for oleic acid C18:1 (three QTLs, 25% for linoleic acid C18:2 (one QTL, and 40% (two QTLs for the iodine value. Good marker co-linearity was observed between the intraspecific and interspecific Simple Sequence Repeat (SSR linkage maps. Specific QTL regions for several traits were found in each mapping population. Our comparative QTL results in both E. guineensis and interspecific materials strongly suggest that, apart from two common QTL zones, there are two specific QTL regions with major effects, which might be one in E. guineensis, the other in E. oleifera, which are independent of each other and harbor QTLs for several traits, indicating either pleiotropic effects or linkage. Using QTL maps connected by highly transferable SSR markers, our study established a good basis to decipher in the future such hypothesis at the Elaeis genus level.

  19. Physiological and morphological characterization of american oil palms (elaeis oleifera hbk cortes) and their hybrids (elaeis oleifera × elaeis guineensis) on the indupalma plantation

    Yurany Dayanna Rivera M; Daniel Gerardo Cayón S; Jesús Edgardo López M

    2013-01-01

    The morphological and photosynthetic activity were characterized in six genotypes of American oil palm (E. oleifera) of different origins and three of their OxG hybrids (E. oleifera × E. guineensis) with a completely randomized experimental design, three replications and 16 palms per replication. Determinations were made for: photosynthesis, internal CO2 concentration, chlorophyll content, stomatal openings and density, specific leaf area, and measurements of vegetative growth. The genotypes ...

  20. Toxicological assessment of crude palm oil (Elaeis guineensis Jacq. used in deep frying of akara (cowpea paste finger food

    Felzenszwalb, I.

    2014-06-01

    Full Text Available Akara is cowpea paste which is deep-fried in crude palm oil (CPO; Elaeis guineensis Jacq. and sold as a street finger food in Brazil and Africa. During the food frying oils can form toxic decomposition products as total polar compounds (TPC, which can determinate oil degradation. The aim of this study was to assess the toxicity of CPO used in akara frying for 25 hours. Changes in the oil were determined by TPC quantification and mutagenicity using a Salmonella/microssome assay with Salmonella Typhimurium strains TA97, TA98, TA100 and TA102 with and without exogenous metabolic activation. Assuming that 25% TPC is the maximum level permitted in frying oils and it ranged from 14.08 to 29.81%, frying palm oil exceeded the limit. Nonetheless, no cytotoxic, mutagenic or genotoxic activity were detected in CPO used in the traditional akara frying process.Akara es una tapa hecha de pasta de frijol frito en aceite de palma crudo (CPO; Elaeis guineensis, que se vende en las calles de Brasil y África. Durante la fritura de alimentos, los aceites pueden formar productos de descomposición tóxicos como los compuestos polares totales (TPC, que determinan la degradación del aceite. El objetivo de este estudio fue evaluar la toxicidad de CPO utilizado en 25 horas de frituras de akara. Los cambios en el aceite se determinaron mediante la cuantificación de TPC y ensayos de mutagenicidad en Salmonella microsomas usando cepas de Salmonella Typhimurium TA97, TA98, TA100 y TA102 con y sin activación metabólica exógena. Se asume que el 25% de TPC es el nivel máximo permitido, los aceites de fritura oscilaron desde 14,08 hasta 29,81%. Ningún CPO utilizado en el proceso de akara tradicional mostró ser citotóxico, ni tener actividad mutagénica o genotóxica.

  1. Cloning of nitric oxide associated 1 (NOA1) transcript from oil palm (Elaeis guineensis) and its expression during Ganoderma infection.

    Kwan, Yee-Min; Meon, Sariah; Ho, Chai-Ling; Wong, Mui-Yun

    2015-02-01

    Nitric oxide associated 1 (NOA1) protein is implicated in plant disease resistance and nitric oxide (NO) biosynthesis. A full-length cDNA encoding of NOA1 protein from oil palm (Elaeis guineensis) was isolated and designated as EgNOA1. Sequence analysis suggested that EgNOA1 was a circular permutated GTPase with high similarity to the bacterial YqeH protein of the YawG/YlqF family. The gene expression of EgNOA1 and NO production in oil palm root tissues treated with Ganoderma boninense, the causal agent of basal stem rot (BSR) disease were profiled to investigate the involvement of EgNOA1 during fungal infection and association with NO biosynthesis. Real-time PCR (qPCR) analysis revealed that the transcript abundance of EgNOA1 in root tissues was increased by G. boninense treatment. NO burst in Ganoderma-treated root tissue was detected using Griess reagent, in advance of the up-regulation of the EgNOA1 transcript. This indicates that NO production was independent of EgNOA1. However, the induced expression of EgNOA1 in Ganoderma-treated root tissues implies that it might be involved in plant defense responses against pathogen infection. PMID:25462975

  2. Isolation, expression analysis and characterization of EgNDL, a NDR-like protein in oil palm (Elaeis guineensis Jacq.

    Kedsirin Ruttajorn

    2016-06-01

    Full Text Available A novel cDNA of a SF21-like protein or NDR-like protein (EgNDL from Elaeis guineensis Jacq. is 1,044 bp in length and encodes a putative protein with a 347-amino-acid open reading frame. The EgNDL showed 93% identity to the pollenspecific SF21-like protein of Phoenix dactylifera and also showed 79% identity to the NDL protein of Theobroma cacao. Expression analysis of the EgNDL gene in various tissues showed that EgNDL was expressed in the anthers, pistils, mesocarp and leaves. The high expression of EgNDL among three oil palm varieties was significantly expressed in Pisifera (P<0.05, which is commonly used as a male parent in crosses. Computational tools were used to predict the protein and concluded that EgNDL is a putative membrane protein that may function in a signal transduction pathway during pollen development. In this context, knowledge regarding EgNDL and its potential role in plant developmental processes will benefit oil palm breeding programs.

  3. Purification and characterisation of acyl-CoA: glycerol 3-phosphate acyltransferase from oil palm (Elaeis guineensis) tissues.

    Manaf, A M; Harwood, J L

    2000-01-01

    Glycerol 3-phosphate acyltransferase (GPAT, EC 2.3.15) catalyses the first step of the Kennedy pathway for acyl lipid formation. This enzyme was studied using high-speed particulate fractions from oil palm (Elaeis guineensis Jacq.) tissue cultures and mesocarp acetone powders. The fractions were incubated with [(14)C]glycerol 3-phosphate and incorporation of radioactivity into Kennedy pathway intermediates studied. Optimal conditions were broadly similar between the two preparations but those from fruit mesocarp clearly contained more active enzymes for the subsequent stages of the Kennedy pathway - as exemplified by the appreciable accumulation of radioactivity in triacylglycerol. Experiments with different acyl-CoA substrates showed that the GPAT in both high-speed particulate preparations had a significant preference for palmitate. Glycerol 3-phosphate acyltransferase was solubilised from both preparations with optimal solubilisation being achieved at 0.5% (w/v) CHAPS concentrations. Solubilised GPATs were purified further using DE52 ion-exchange chromatography and Sephadex G-100 molecular exclusion chromatography. Purifications of up to about 70-fold were achieved. The purified GPATs showed a strong preference for palmitoyl-CoA compared to other acyl-CoA donors, in keeping with the importance of palmitate in palm oil. PMID:10664139

  4. Supplémentation de feuilles de palmier (Elaeis guineensis) traitées à la vapeur à des agneaux en Malaisie

    Bengaly, K

    2015-01-01

    Supplementation of Steamprocessed Oil Palm (Elaeis guineensis) Fronds for Lambs in Malaysia. The effect of supplementation of steam-treated oil palm fronds (SOPF) at an optimum rate of urea inclusion (16 g/kg) together with graded levels of pelleted cassava foliage (PCF) on nitrogen balance in lambs was investigated. The PCF was fed in amounts equivalent to 0% (PCF0), 20% (PCF20) or 40% (PCF40) of dry matter (DM) intake of steam-treated oil palm fronds to 9 lambs in a completely randomized bl...

  5. Exploiting transcriptome data for the development and characterization of gene-based SSR markers related to cold tolerance in oil palm (Elaeis guineensis)

    Xiao, Yong; Zhou, Lixia; Xia, Wei; Mason, Annaliese S.; Yang, Yaodong; Ma, Zilong; Peng, Ming

    2014-01-01

    Background The oil palm (Elaeis guineensis, 2n = 32) has the highest oil yield of any crop species, as well as comprising the richest dietary source of provitamin A. For the tropical species, the best mean growth temperature is about 27°C, with a minimal growth temperature of 15°C. Hence, the plantation area is limited into the geographical ranges of 10°N to 10°S. Enhancing cold tolerance capability will increase the total cultivation area and subsequently oil productivity of this tropical sp...

  6. Pengaruh Komposisi Media Tanam Serta Pemberian Limbah Cair Pabrik Kelapa Sawit Kolam Aerob Terhadap Pertumbuhan Bibit Kelapa Sawit (Elaeis guineensis Jacq.) Di Pre-Nursery

    Manurung, Firman K.

    2016-01-01

    The availability of top soil as an oil palm sead plant media is very limited, so to contend it is used sub soil plant media which more available and easier to get it, but it has bad chemical characteristics. Application Oil Palm Empty Bunch (OPEB) compost or Palm Oil Liquid Waste) aerobic pond is expected to bost growth of Palm Seed (Elaeis guineensis Jacq.).This research had been conducted At Rumah Kasa of Fakultas Pertanian USU in October 2013 until January 2014,, using factorial rando...

  7. Toxicological assessment of crude palm oil (Elaeis guineensis Jacq.) used in deep frying of akara (cowpea paste finger food)

    Felzenszwalb, I.; da Costa Mazzei, J. L.; Feitosa, S.; Fortes Aiub, C. A.; de Almeida, D. T.

    2014-01-01

    Akara is cowpea paste which is deep-fried in crude palm oil (CPO; Elaeis guineensis Jacq.) and sold as a street finger food in Brazil and Africa. During the food frying oils can form toxic decomposition products as total polar compounds (TPC), which can determinate oil degradation. The aim of this study was to assess the toxicity of CPO used in akara frying for 25 hours. Changes in the oil were determined by TPC quantification and mutagenicity using a Salmonella/microssome assay with Salmonel...

  8. Molecular cloning and characterization of the late embryogenesis abundant group 4 (EgLEA4) gene from oil palm (Elaeis guineensis Jacq)

    Watcharasuda Hualkasin; Waleerath Thongin; Kanokon Petsean; Amornrat Phongdara; Alisa Nakkaew

    2013-01-01

    The Late-Embryogenesis Abundant group 4 (LEA4) genes is a group of genes that have been reported to be involved in stress and hormone responses. The completed LEA4 cDNA sequence was first obtained from a set of EST sequences of oil palm (Elaeis guineensis Jacq), named as EgLEA4. The open reading frame is 486 bp in length, encoding a deduced amino acid sequence of 161 residues with a molecular weight of 16.5 kDa and a pI value of about 8.0. Five amino acid motif patterns were found in the EgLE...

  9. Spatial distribution of Metamasius hemipterus (Coleoptera: Curculionidae in planting oil palm (Elaeis guineensis Jacq in Roraima = Distribuição espacial de Metamasius hemipterus (Coleoptera: Curculionidae em plantio de dendê (Elaeis guineensis Jacq em Roraima

    Antonio Cesar Silva Lima

    2015-10-01

    Full Text Available The aim of this study was to evaluate the spatial distribution pattern and aggregation radius of Metamasius hemipterus in an oil palm (Elaeis guineensis plantation, in the state of Roraima, Brazil (RR. The experimental area is located in the town of São João da Baliza, RR, where 24 samples were taken from September 2013 to August 2014. The perimeter of the area was marked out using the global positioning system, with sampling grids of 45 x 45 m. Spatial distribution was determined by placing a bucket-type trap at each sampling point, using food (sugarcane as bait, and values obtained for the variable, number of insects/ trap, together with the latitude and longitude. An analysis of variability and spatial dependence was carried out by incorporating geostatistical procedures based on spatial modelling techniques employing semivariograms. In addition, kriging maps were generated from counts of adult M. hemipterus taken in the field. The sampling grids proved to be suitable for characterising the spatial distribution in the field of M. hemipterus It was found that the spatial distribution of M. hemipterus is aggregated, with a spatial dependence described by the spherical model, forming patches of 78 to 199 m (range of the model. By means of the kriging maps, it could be seen that infestation first starts at the edges of the plantation, later spreading throughout the area. The results suggest that traps should be placed along the edges of the plantation, to collect and control the insect pests. = Objetivou-se com o presente trabalho avaliar o padrão de distribuição espacial, bem como o raio de agregação de Metamasius hemipterus em plantio de dendê (Elaeis guineensis, no estado de Roraima. A área experimental está localizada no município de São João da Baliza, RR, onde foram realizadas 24 amostragens no período de setembro de 2013 a agosto de 2014. O perímetro da área foi demarcado com receptor de sistema de posicionamento global, e

  10. Spatial distribution of Metamasius hemipterus (Coleoptera: Curculionidae in an oil palm plantation (Elaeis guineensis Jacq in Roraima = Distribuição espacial de Metamasius hemipterus (Coleoptera: Curculionidae em plantio de dendê (Elaeis guineensis Jacq em Roraima

    Luiz Fernandes Silva Dionisio

    2015-10-01

    Full Text Available The aim of this study was to evaluate the spatial distribution pattern and aggregation radius of Metamasius hemipterus in an oil palm (Elaeis guineensis plantation, in the state of Roraima, Brazil (RR. The experimental area is located in the town of São João da Baliza, RR, where 24 samples were taken from September 2013 to August 2014. The perimeter of the area was marked out using the global positioning system, with sampling grids of 45 x 45 m. Spatial distribution was determined by placing a bucket-type trap at each sampling point, using food (sugarcane as bait, and values obtained for the variable, number of insects/ trap, together with the latitude and longitude. An analysis of variability and spatial dependence was carried out by incorporating geostatistical procedures based on spatial modelling techniques employing semivariograms. In addition, kriging maps were generated from counts of adult M. hemipterus taken in the field. The sampling grids proved to be suitable for characterising the spatial distribution in the field of M. hemipterus It was found that the spatial distribution of M. hemipterus is aggregated, with a spatial dependence described by the spherical model, forming patches of 78 to 199 m (range of the model. By means of the kriging maps, it could be seen that infestation first starts at the edges of the plantation, later spreading throughout the area. The results suggest that traps should be placed along the edges of the plantation, to collect and control the insect pests = Objetivou-se com o presente trabalho avaliar o padrão de distribuição espacial, bem como o raio de agregação de Metamasius hemipterus em plantio de dendê (Elaeis guineensis, no estado de Roraima. A área experimental está localizada no município de São João da Baliza, RR, onde foram realizadas 24 amostragens no período de setembro de 2013 a agosto de 2014. O perímetro da área foi demarcado com receptor de sistema de posicionamento global, e

  11. EFECTO DE HONGOS MICORRIZICOS ARBUSCULARES EN PLÁNTULAS DE Elaeis guineensis (Palmaceae CON ALTO NIVEL DE P EN EL SUELO EFFECT OF ARBUSCULAR MYCORRHIZAL FUNGI IN Elaeis guineensis (Palmaceae SEEDLINGS WITH HIGH PHOSPHORUS LEVEL IN THE SOIL

    SILVIA EUGENIA BARRERA BERDUGO

    Full Text Available Los hongos micorrízicos arbusculares (HMA facilitan la absorción de nutrientes a las plantas hospederas, por esta razón estos microorganismos cumplen un rol fundamental en el funcionamiento de los agroecosistemas. El objetivo de este trabajo fue evaluar el efecto de la asociación simbiótica entre HMA nativos y comerciales y plántulas de Elaeis guineensis en condiciones de vivero con un nivel alto de fósforo (P en el suelo. Plantas de tres meses de edad fueron sometidas a cuatro tratamientos: Inóculo Nativo (IN, Inóculo Comercial (IC, Mixto (M y Testigo absoluto (TA. Se evaluaron los parámetros: peso seco total de la planta, peso seco raíz, peso seco parte aérea de la planta, altura de la planta, tasa de crecimiento relativo, colonización micorrízica y número de esporas en el suelo. Se realizaron tres muestreos, uno inicial (día 0, uno a los 45 y a los 90 días después del trasplante de las plántulas (ddt. Los datos fueron analizados mediante un ANOVA o Kruskall-Wallis según el comportamiento de los datos, seguido de un test de Duncan para comparar las medias o un test modificado de Tuckey para datos no paramétricos. Se observaron diferencias significativas en el número de esporas entre los tratamientos IN, M y TA, a los 45 ddt. Para la variable colonización micorrízica se observaron diferencias significativas a los 45 ddt entre los tratamientos IC y TA, mientras que a los 90 ddt se presentaron diferencias entre los tratamientos IN, IC y M, con respecto al TA. A pesar de que el nivel de P en el suelo fue alto, el porcentaje de colonización micorrízica estuvo por encima del 50% en los muestreos realizados a los 45 y 90 días. El tratamiento IN funcionó mejor que los tratamientos IC y M, para las condiciones edáficas de este experimento.The arbuscular mycorrhizal fungi (AMF facilitate the absorption of nutrients to the host plants. These microorganisms therefore, fulfill a fundamental roll in the operation of

  12. RNA-seq analysis of oil palm under cold stress reveals a different C-repeat binding factor (CBF) mediated gene expression pattern in Elaeis guineensis compared to other species.

    Lei, Xintao; Xiao, Yong; Xia, Wei; Mason, Annaliese S; Yang, Yaodong; Ma, Zilong; Peng, Ming

    2014-01-01

    Elaeis guineensis as a tropical oil-crop is particularly sensitive to low temperature. Improvement of cold-tolerance may significantly increase the total cultivation area of this tropical oil-crop worldwide. We sequenced cold-treated and control (untreated) samples of Elaeis guineensis. De novo assembly generated 51,452 unigenes with an average length of 703 bp. Subsequently, these expressed sequences were functionally annotated. In the K category (transcription factors) of COG (Cluster of Orthologous Group) annotation, the largest proportion of genes induced and repressed at least two-fold under cold stress were from the AP2/ERE family, indicating that C-repeat binding factor, (CBFs, members of the AP2/ERE family) may play a central role in cold tolerance in Elaeis guineensis. Subsequently, the CBF-mediated signal transduction pathway was reconstructed based on transcriptome data and the gene expression profile involving the pathway was examined using real-time quantitative RT-PCR (qRT-PCR). CBFs reached maximum transcript level both at medium (4 h) and long period time points (7 days), contrary to the expression pattern of CBFs in Arabidopsis and rice. Moreover, the promoters of downstream Cold Responsive gene (CORs) regulated by CBFs were analyzed. Conservation, mutation and absence of the DRE core motif were detected in the promoters of six CORs. These mutations in DRE motifs suggest that CORs may not be induced via cold stress in Elaeis guineensis. PMID:25479236

  13. RNA-seq analysis of oil palm under cold stress reveals a different C-repeat binding factor (CBF mediated gene expression pattern in Elaeis guineensis compared to other species.

    Xintao Lei

    Full Text Available Elaeis guineensis as a tropical oil-crop is particularly sensitive to low temperature. Improvement of cold-tolerance may significantly increase the total cultivation area of this tropical oil-crop worldwide. We sequenced cold-treated and control (untreated samples of Elaeis guineensis. De novo assembly generated 51,452 unigenes with an average length of 703 bp. Subsequently, these expressed sequences were functionally annotated. In the K category (transcription factors of COG (Cluster of Orthologous Group annotation, the largest proportion of genes induced and repressed at least two-fold under cold stress were from the AP2/ERE family, indicating that C-repeat binding factor, (CBFs, members of the AP2/ERE family may play a central role in cold tolerance in Elaeis guineensis. Subsequently, the CBF-mediated signal transduction pathway was reconstructed based on transcriptome data and the gene expression profile involving the pathway was examined using real-time quantitative RT-PCR (qRT-PCR. CBFs reached maximum transcript level both at medium (4 h and long period time points (7 days, contrary to the expression pattern of CBFs in Arabidopsis and rice. Moreover, the promoters of downstream Cold Responsive gene (CORs regulated by CBFs were analyzed. Conservation, mutation and absence of the DRE core motif were detected in the promoters of six CORs. These mutations in DRE motifs suggest that CORs may not be induced via cold stress in Elaeis guineensis.

  14. Bionomics data and descriptions of the immatures of Calyptocephala gerstaeckeri Boheman (Coleoptera: Chrysomelidae), pest of the oil palm (Elaeis guineensis J.) and camedor palm (Chamaedorea elegans Mart.) (Arecaceae) in Tabasco, Mexico

    Characters of the external morphology of egg, larval instars and pupae of Calyptocephala gerstaeckeri Boheman, insect pest of oil palm (Elaeis guineensis J.) and camedor palm (Chamaedorea elegans Mart.) in the State of Tabasco, Mexico, are described and illustrated. Some bionomics data are also presented. The specie was reared in the laboratory on young oil palm plants. (author)

  15. Balok Laminasi Dengan Kombinasi Dari Batang Kelapa Sawit (Elaeis Guineensis Jacq) Dan Kayu Mahoni (Swietenia Mahagoni)

    Ginting, Denni Ardian R.

    2013-01-01

    ABSTRACT The use of timber as a structural material is not only limited to solid timber but also as laminate called lamination wood (gluelam). This research explored the optimum total of layers, and height of the oil-palm stem to evaluate physis and mechanis properties of lamination wood which is a combination of oil-palm stem (Elaies guineensis jacq) and mahoni (Swietenia mahagoni). The lamination wood had 50 cm long, 5 cm wide, and 5 cm deep. Glue spread are 300 g/m2 with double side ...

  16. Fraccionamiento e interesterificacion del aceite de palma (Elaeis guineensis cultivado en la amazonia peruana

    Mancini Filho, Jorge

    2008-06-01

    Full Text Available In the present work, the physical and chemical characteristics of the fruit of the oily palm coming from the river basin of the Manití (Region Loreto - Peru were studied. Also, the fractionation of the palm oil and the interesterification of mixtures of palm oil/estearin was carried out. Physico- chemical properties of the crude oil and of the products obtained and fatty acids were analysed by gas chromatography. The level of saturated fatty acids increased from 51,17% in the palm oil to 54,31% in the stearin. The best products for the food industry were the interesterified samples as they had melting points close to 37 °C.En el presente trabajo se realizó el estudio de las características físicas y químicas del fruto de la palma aceitera procedente de la cuenca del Manití. (Región Loreto - Perú. Del mismo modo se realizó el fraccionamiento e interesterificación de las mezclas de aceite de palma y estearina en las proporciones. Sobre el aceite crudo y los productos se determinaron las propiedades físico-químicos y análisis de ácidos grasos mediante la cromatografía gaseosa. El aceite de palma presenta una concentración de ácido grasos saturados de 51,17% y cuando fraccionado a 25 °C, este se incrementa en la estearina a 54,31%. Los mejores productos para la industria de alimentos son las mezclas interesterificadas de estearina tanto sola como con sus mezclas con aceite de palma, dado que presentan puntos de fusión próximos a 37 °C.

  17. Feeding oil palm (Elaeis guineensis, Jacq. fronds alters rumen protozoal population and ruminal fermentation pattern in goats

    Mahdi Ebrahimi

    2015-07-01

    Full Text Available Oil palm fronds (OPF, normally available all the year round, may provide a sustainable ruminant feed for livestock industry in tropical regions. A feeding trial was conducted to study the effects of feeding OPF on the rumen protozoal population, rumen fermentation and fatty acid profiles of rumen fluid in goats. Twentyfour five-month-old Kacang crossbred male goats were individually housed and fed for 100 d with concentrate diets supplemented with oil palm (Elaeis guineensis Jacq. frond pellets. The treatments were: CON (100% concentrate, MOPF (75% concentrate + 25% OPF, w/w and HOPF (50% concentrate + 50% OPF, w/w. The diets were adjusted to be isocaloric. The pH of rumen fluid was in the order of HOPF (5.90>MOPF (5.74>CON (5.62. Both HOPF (17.75x104/mL and MOPF (17.13x104/mL had significantly (P<0.05 higher population of Entodinium sp. than CON (14.88x104/mL. Although populations of Holotrichs and total protozoa among the three groups did not show any significant difference (P>0.05, populations were in the numerical order of HOPF>MOPF>CON. The molar proportions of acetate were significantly higher (P<0.05 in HOPF animals compared to MOPF and CON. The altered status in the rumen environment due to supplementation of OPF in the diets resulted in the highest (P<0.05 amount of unsaturated fatty acids (UFA in the rumen of animals receiving HOPF and MOPF diet. These results were suggestive of a decreased biohydrogenation in the rumen, resulting in higher levels of UFA available for hindgut absorption, and hence their increased incorporation in the plasma and edible tissues of the HOPF animals.

  18. Improved cryopreservation of oil palm (Elaeis guineensis Jacq.) polyembryoids using droplet vitrification approach and assessment of genetic fidelity.

    Gantait, Saikat; Sinniah, Uma Rani; Suranthran, Periasamy; Palanyandy, Sharrmila Rengeswari; Subramaniam, Sreeramanan

    2015-01-01

    In the present study, polyembryoids of oil palm (Elaeis guineensis Jacq.) were cryopreserved with successful revival of 68 % for the first time using the droplet vitrification technique. Excised polyembryoids (3-5-mm diameter) from 3-month-old in vitro cultures were pre-cultured for 12 h in liquid Murashige and Skoog medium supplemented with 0.5 M sucrose. The polyembryoids were osmoprotected in loading solution [10% (w/v) dimethyl sulphoxide (DMSO) plus 0.7 M sucrose] for 30 min at room temperature and then placed on aluminium strips where they were individually drenched in chilled droplets of vitrification solution (PVS2) [30% (w/v) glycerol plus 15% (w/v) ethylene glycol (EG) plus 15% (w/v) DMSO plus 0.4 M sucrose] for 10 min. The aluminium strips were enclosed in cryovials which were then plunged quickly into liquid nitrogen and kept there for 1 h. The polyembryoids were then thawed and unloaded (using 1.2 M sucrose solution) with subsequent transfer to regeneration medium and stored in zero irradiance. Following for 10 days of storage, polyembryoids were cultured under 16 h photoperiod of 50 μmol m(-2) s(-1) photosynthetic photon flux density, at 23 ± 1 °C. Post-thaw growth recovery of 68% was recorded within 2 weeks of culture, and new shoot development was observed at 4 weeks of growth. Scanning electron microscopy revealed that successful regeneration of cryopreserved polyembryoids was related to maintenance of cellular integrity, presumably through PVS2 exposure for 10 min. The present study demonstrated that cryopreservation by droplet vitrification enhanced the regeneration percentages of oil palm in comparison with the conventional vitrification method previously reported. PMID:24893588

  19. Analysis of multiple transcriptomes of the African oil palm (Elaeis guineensis) to identify reference genes for RT-qPCR.

    Xia, Wei; Mason, Annaliese S; Xiao, Yong; Liu, Zheng; Yang, Yaodong; Lei, Xintao; Wu, Xiaoming; Ma, Zilong; Peng, Ming

    2014-08-20

    The African oil palm (Elaeis guineensis), which is grown in tropical and subtropical regions, is a highly productive oil-bearing crop. For gene expression-based analyses such as reverse transcription-quantitative real time PCR (RT-qPCR), reference genes are essential to provide a baseline with which to quantify relative gene expression. Normalization using reliable reference genes is critical in correctly interpreting expression data from RT-qPCR. In order to identify suitable reference genes in African oil palm, 17 transcriptomes of different tissues obtained from NCBI were systematically assessed for gene expression variation. In total, 53 putative candidate reference genes with coefficient of variation values <3.0 were identified: 18 in reproductive tissue and 35 in vegetative tissue. Analysis for enriched functions showed that approximately 90% of identified genes were clustered in cell component gene functions, and 12 out of 53 genes were traditional housekeeping genes. We selected and validated 16 reference genes chosen from leaf tissue transcriptomes by using RT-qPCR in sets of cold, drought and high salinity treated samples, and ranked expression stability using statistical algorithms geNorm, Normfinder and Bestkeeper. Genes encoding actin, adenine phosphoribosyltransferase and eukaryotic initiation factor 4A genes were the most stable genes over the cold, drought and high salinity stresses. Identification of stably expressed genes as reference gene candidates from multiple transcriptome datasets was found to be reliable and efficient, and some traditional housekeeping genes were more stably expressed than others. We provide a useful molecular genetic resource for future gene expression studies in African oil palm, facilitating molecular genetics approaches for crop improvement in this species. PMID:24862192

  20. Effect of preservation methods of oil palm sap (Elaeis guineensis) on the reproductive indices of male wistar rats.

    Ikegwu, Theophilus Maduabuchukwu; Okafor, Gabriel Ifeanyi; Ochiogu, Izuchukwu Shedrack

    2014-12-01

    Thirty male Wistar rats, split into five groups of six rats each, were administered different forms of oil palm tree (Elaeis guineensis) sap samples by gavage based on 1.5% of their weekly body weights. Group 1 which served as control received only water, group 2 received pasteurized palm sap (PPS), group 3 received market palm wine (MPW), group 4 received frozen palm sap (FPS), whereas group 5 received fresh palm sap (FrPS). Chemical composition of the sap samples was determined. Normal feed and water were fed ad libitum. After 2 months of treatment, each male rat group was allowed 7 days to mate with six female Wistar rats. Thereafter, blood and epididymal samples were collected for testosterone assay and sperm count, respectively, before they were humanely sacrificed and testicular tissues taken for testicular histology. Litter weight and size of the pups produced by the females of each group were determined at birth. The sap samples contained carbohydrate (0.01-11.71%), protein (1.56-1.95%), ash (0.22-0.35%), moisture (92.55-98.24%), and alcohol (0.26-3.50%). PPS-treated rat group had significantly (P<.05) decreased sperm count (42.60±23.64×10(6)), abnormal increase in testosterone level, and necrosis in the histology of the testes with reduced spermatogenetic activity, compared with other treatment groups. The female rats crossed with male rats fed on FrPS or FPS produced the highest number of pups followed by the control group. This study demonstrated that the intake of FrPS improved fertility in male animals, but its administration for a long period led to necrotic changes in the testes, whereas pasteurization of palm sap, impacted negatively on the reproductive indices of male animals. PMID:25101691

  1. In vitro anti-plasmodial activity of three herbal remedies for malaria in Ghana: Adenia cissampeloides (Planch. Harms., Termina liaivorensis A. Chev, and Elaeis guineensis Jacq

    Kofi Annan

    2012-01-01

    Full Text Available Background: Herbal remedies of Adenia cissampeloides, Terminalia ivorensis, and Elaeis guineensis among others have been used in Ghana for the treatment of various ailments including malaria. However, most of these remedies have not been scientifically investigated. Objective: This study, therefore, seeks to investigate the anti-plasmodial activity of these plants. Materials and Methods: The ethanolic extracts of A. cissampeloides stem, T. ivorensis stem bark, and E. guineensis leaves were tested for in vitro anti-plasmodial activity against chloroquine-resistant strains of Plasmodium falciparum. Thin blood films were used to assess the level of parasitemia and growth inhibition of the extracts. Results: The IC 50 of A. cissampeloides, T. ivorensis, and E. guineensis were 8.521, 6.949, and 1.195 μg/ml, respectively, compared to artesunate with IC 50 of 0.031 μg/ml. Conclusion: The result of this study appears to confirm the folkloric anti-malarial use these plants.

  2. The Antimicrobial Efficacy of Elaeis guineensis: Characterization, in Vitro and in Vivo Studies

    Sreenivasan Sasidharan

    2012-04-01

    Full Text Available The urgent need to treat multi-drug resistant pathogenic microorganisms in chronically infected patients has given rise to the development of new antimicrobials from natural resources. We have tested Elaeis guineensis Jacq (Arecaceae methanol extract against a variety of bacterial, fungal and yeast strains associated with infections. Our studies have demonstrated that E. guineensis exhibits excellent antimicrobial activity in vitro and in vivo against the bacterial and fungal strains tested. A marked inhibitory effect of the E. guineensis extracts was observed against C. albicans whereby E. guineensis extract at ½, 1, or 2 times the MIC significantly inhibited C. albicans growth with a noticeable drop in optical density (OD of the bacterial culture. This finding confirmed the anticandidal activity of the extract on C. albicans. Imaging using scanning (SEM and transmission (TEM electron microscopy was done to determine the major alterations in the microstructure of the extract-treated C. albicans. The main abnormalities noted via SEM and TEM studies were the alteration in morphology of the yeast cells. In vivo antimicrobial activity was studies in mice that had been inoculated with C. albicans and exhibited good anticandidal activity. The authors conclude that the extract may be used as a candidate for the development of anticandidal agent.

  3. Role of transitory carbon reserves during adjustment to climate variability and source-sink imbalances in oil palm (Elaeis guineensis).

    Legros, S; Mialet-Serra, I; Clement-Vidal, A; Caliman, J-P; Siregar, F A; Fabre, D; Dingkuhn, M

    2009-10-01

    Oil palm (Elaeis guineensis Jacq.) is a perennial, tropical, monocotyledonous plant characterized by simple architecture and low phenotypic plasticity, but marked by long development cycles of individual phytomers (a pair of one leaf and one inflorescence at its axil). Environmental effects on vegetative or reproductive sinks occur with various time lags depending on the process affected, causing source-sink imbalances. This study investigated how the two instantaneous sources of carbon assimilates, CO(2) assimilation and mobilization of transitory non-structural carbohydrate (NSC) reserves, may buffer such imbalances. An experiment was conducted in Indonesia during a 22-month period (from July 2006 to May 2008) at two contrasting locations (Kandista and Batu Mulia) using two treatments (control and complete fruit pruning treatment) in Kandista. Measurements included leaf gas exchange, dynamics of NSC reserves and dynamics of structural aboveground vegetative growth (SVG) and reproductive growth. Drought was estimated from a simulated fraction of transpirable soil water. The main sources of variation in source-sink relationships were (i) short-term reductions in light-saturated leaf CO(2) assimilation rate (A(max)) during seasonal drought periods, particularly in Batu Mulia; (ii) rapid responses of SVG rate to drought; and (iii) marked lag periods between 16 and 29 months of environmental effects on the development of reproductive sinks. The resulting source-sink imbalances were buffered by fluctuations in NSC reserves in the stem, which mainly consisted of glucose and starch. Starch was the main buffer for sink variations, whereas glucose dynamics remained unexplained. Even under strong sink limitation, no negative feedback on A(max) was observed. In conclusion, the different lag periods for environmental effects on assimilate sources and sinks in oil palm are mainly buffered by NSC accumulation in the stem, which can attain 50% (dw:dw) in stem tops. The resulting

  4. Genetic Architecture of Palm Oil Fatty Acid Composition in Cultivated Oil Palm (Elaeis guineensis Jacq.) Compared to Its Wild Relative E. oleifera (H.B.K) Cortés

    Carmenza Montoya; Benoit Cochard; Albert Flori; David Cros; Ricardo Lopes; Teresa Cuellar; Sandra Espeout; Indra Syaputra; Pierre Villeneuve; Michel Pina; Enrique Ritter; Thierry Leroy; Norbert Billotte

    2014-01-01

    We searched for quantitative trait loci (QTL) associated with the palm oil fatty acid composition of mature fruits of the oil palm E. guineensis Jacq. in comparison with its wild relative E. oleifera (H.B.K) Cortés. The oil palm cross LM2T x DA10D between two heterozygous parents was considered in our experiment as an intraspecific representative of E. guineensis. Its QTLs were compared to QTLs published for the same traits in an interspecific Elaeis pseudo-backcross used as an indirect repre...

  5. Climate-induced vegetation dynamics and the Bantu Expansion: Evidence from Bantu names for pioneer trees (Elaeis guineensis, Canarium schweinfurthii, and Musanga cecropioides)

    Bostoen, Koen; Grollemund, Rebecca; Koni Muluwa, Joseph

    2013-07-01

    The present article examines whether Late Holocene climate-induced vegetation changes in the Central African forest block may have facilitated the Bantu Expansion. This is done through a body of evidence that is not commonly used for the reconstruction of vegetation dynamics, i.e. language data. The article focuses on common Bantu vocabulary for three pioneer species abundantly present in the Central African pollen record between ca. 2500 and 2000 BP: Musanga cecropioides, Elaeis guineensis, and Canarium schweinfurthii. The geographical distribution patterns of the vernacular names for these pioneer trees add weight to the hypothesis according to which the rainforest contraction that emerged in the first millennium BC had an impact on the way Bantu languages dispersed.

  6. Distribuição espacial de Metamasius hemipterus (Coleoptera: Curculionidae) em plantio de dendê (Elaeis guineensis Jacq) em Roraima

    Silva Dionisio, Luiz Fernandes; Silva Lima, Antonio Cesar; Fidelis de Morais, Elisangela Gomes; Correia, Ruy Guilherme; Ferreira dos Santos, Artur Vinícius; dos Santos Ximenes, Carla Klis

    2015-01-01

    Objetivou-se com o presente trabalho avaliar o padrão de distribuição espacial, bem como o raio de agregação de Metamasius hemipterus em plantio de dendê (Elaeis guineensis), no estado de Roraima. A área experimental está localizada no município de São João da Baliza, RR, onde foram realizadas 24 amostragens no período de setembro de 2013 a agosto de 2014. O perímetro da área foi demarcado com receptor de sistema de posicionamento global, e os grides de amostragem tiveram dimensão de 45 x 45 ...

  7. Diffusion variétale du palmier à huile (Elaeis guineensis Jacq.

    Durand-Gasselin Tristan

    2000-03-01

    Full Text Available La productivité du palmier à huile au cours du siècle passé a connu une croissance importante où l’amélioration génétique a pris une large part. Elle a connu un rythme de croissance assez comparable à celui obtenu pour des plantes annuelles de pays tempérés (blé, maïs, tournesol, etc.. Il est possible de dégager les étapes importantes qui ont marqué l’histoire de l’amélioration. Après une assez longue période de sélection massale, l’exploitation du gène « shell », dont l’hérédité a été découverte par Beirnaert en 1939, a apporté une amélioration de 30% [1]. Vers 1960, l’exploitation de l’hétérosis des croisements inter-origines apporte une nouvelle amélioration de 10% environ [2]. Enfin, deux cycles de sélection récurrente ont été achevés par différentes équipes et chacun a apporté 12 à 18% d’amélioration de la productivité. En 1991, la valeur moyenne des semences commerciales, plantées dans de bonnes conditions, était proche de 6,7 tonnes d’huile par hectare et par an [3]. Aujourd’hui, elle est de l’ordre de 7,2 t/ha/an [4]. Ces gains quantitatifs ont été accompagnés de progrès qualitatifs importants comme la diminution de la vitesse de croissance, l’augmentation du pourcentage d’huile dans les régimes ou l’acquisition d’une forte résistance à la fusariose en Afrique de l’Ouest [5]. La qualité du futur matériel végétal devrait être sensiblement améliorée par l’utilisation d’une base génétique élargie dans les programmes d’amélioration [6]. En effet, les stations de recherche disposent d’une bonne diversité génétique rassemblée lors de nombreuses prospections réalisées en Afrique (pour E. guineensis et sur une espèce apparentée en Amérique latine (pour E. oleifera. Ces collections apportent de nouveaux caractères de qualité de l’huile, de résistance à la fusariose, qui sévit en Afrique, ou à la pourriture du cœur en Am

  8. Le palmier à huile ({Elaeis guineensis}) et les noyaux de biodiversité des forêts-galeries de Guinée maritime : à propos du commensalisme de l'homme et du chimpanzé

    Leciak, Elisabeth; Hladik, Annette; Hladik, Claude Marcel

    2005-01-01

    The oil palm (Elaeis guineensis), occurring with a remarkably high density in the northwestern coastal area of Guinea, provides a key resource to the chimpanzee (Pan troglodytes verus), including food and nesting sites. However no evidence of nut cracking has been observed, although potential tools are available and are occasionally used by humans. The abundance of food resources in the gallery forest, and the availability, throughout the year, of the pulp of the oil palm may explain the abse...

  9. Genetic architecture of palm oil fatty acid composition in cultivated oil palm (Elaeis guineensis Jacq.) compared to its wild relative E. oleifera (H.B.K) Cortés.

    Montoya, Carmenza; Cochard, Benoit; Flori, Albert; Cros, David; Lopes, Ricardo; Cuellar, Teresa; Espeout, Sandra; Syaputra, Indra; Villeneuve, Pierre; Pina, Michel; Ritter, Enrique; Leroy, Thierry; Billotte, Norbert

    2014-01-01

    We searched for quantitative trait loci (QTL) associated with the palm oil fatty acid composition of mature fruits of the oil palm E. guineensis Jacq. in comparison with its wild relative E. oleifera (H.B.K) Cortés. The oil palm cross LM2T x DA10D between two heterozygous parents was considered in our experiment as an intraspecific representative of E. guineensis. Its QTLs were compared to QTLs published for the same traits in an interspecific Elaeis pseudo-backcross used as an indirect representative of E. oleifera. Few correlations were found in E. guineensis between pulp fatty acid proportions and yield traits, allowing for the rather independent selection of both types of traits. Sixteen QTLs affecting palm oil fatty acid proportions and iodine value were identified in oil palm. The phenotypic variation explained by the detected QTLs was low to medium in E. guineensis, ranging between 10% and 36%. The explained cumulative variation was 29% for palmitic acid C16:0 (one QTL), 68% for stearic acid C18:0 (two QTLs), 50% for oleic acid C18:1 (three QTLs), 25% for linoleic acid C18:2 (one QTL), and 40% (two QTLs) for the iodine value. Good marker co-linearity was observed between the intraspecific and interspecific Simple Sequence Repeat (SSR) linkage maps. Specific QTL regions for several traits were found in each mapping population. Our comparative QTL results in both E. guineensis and interspecific materials strongly suggest that, apart from two common QTL zones, there are two specific QTL regions with major effects, which might be one in E. guineensis, the other in E. oleifera, which are independent of each other and harbor QTLs for several traits, indicating either pleiotropic effects or linkage. Using QTL maps connected by highly transferable SSR markers, our study established a good basis to decipher in the future such hypothesis at the Elaeis genus level. PMID:24816555

  10. Molecular Characterization of the Elaeis guineensis Medium-Chain Fatty Acid Diacylglycerol Acyltransferase DGAT1-1 by Heterologous Expression in Yarrowia lipolytica.

    Laure Aymé

    Full Text Available Diacylglycerol acyltransferases (DGAT are involved in the acylation of sn-1,2-diacylglycerol. Palm kernel oil, extracted from Elaeis guineensis (oil palm seeds, has a high content of medium-chain fatty acids mainly lauric acid (C12:0. A putative E. guineensis diacylglycerol acyltransferase gene (EgDGAT1-1 is expressed at the onset of lauric acid accumulation in the seed endosperm suggesting that it is a determinant of medium-chain triacylglycerol storage. To test this hypothesis, we thoroughly characterized EgDGAT1-1 activity through functional complementation of a Yarrowia lipolytica mutant strain devoid of neutral lipids. EgDGAT1-1 expression is sufficient to restore triacylglycerol accumulation in neosynthesized lipid droplets. A comparative functional study with Arabidopsis thaliana DGAT1 highlighted contrasting substrate specificities when the recombinant yeast was cultured in lauric acid supplemented medium. The EgDGAT1-1 expressing strain preferentially accumulated medium-chain triacylglycerols whereas AtDGAT1 expression induced long-chain triacylglycerol storage in Y. lipolytica. EgDGAT1-1 localized to the endoplasmic reticulum where TAG biosynthesis takes place. Reestablishing neutral lipid accumulation in the Y. lipolytica mutant strain did not induce major reorganization of the yeast microsomal proteome. Overall, our findings demonstrate that EgDGAT1-1 is an endoplasmic reticulum DGAT with preference for medium-chain fatty acid substrates, in line with its physiological role in palm kernel. The characterized EgDGAT1-1 could be used to promote medium-chain triacylglycerol accumulation in microbial-produced oil for industrial chemicals and cosmetics.

  11. Molecular Characterization of the Elaeis guineensis Medium-Chain Fatty Acid Diacylglycerol Acyltransferase DGAT1-1 by Heterologous Expression in Yarrowia lipolytica.

    Aymé, Laure; Jolivet, Pascale; Nicaud, Jean-Marc; Chardot, Thierry

    2015-01-01

    Diacylglycerol acyltransferases (DGAT) are involved in the acylation of sn-1,2-diacylglycerol. Palm kernel oil, extracted from Elaeis guineensis (oil palm) seeds, has a high content of medium-chain fatty acids mainly lauric acid (C12:0). A putative E. guineensis diacylglycerol acyltransferase gene (EgDGAT1-1) is expressed at the onset of lauric acid accumulation in the seed endosperm suggesting that it is a determinant of medium-chain triacylglycerol storage. To test this hypothesis, we thoroughly characterized EgDGAT1-1 activity through functional complementation of a Yarrowia lipolytica mutant strain devoid of neutral lipids. EgDGAT1-1 expression is sufficient to restore triacylglycerol accumulation in neosynthesized lipid droplets. A comparative functional study with Arabidopsis thaliana DGAT1 highlighted contrasting substrate specificities when the recombinant yeast was cultured in lauric acid supplemented medium. The EgDGAT1-1 expressing strain preferentially accumulated medium-chain triacylglycerols whereas AtDGAT1 expression induced long-chain triacylglycerol storage in Y. lipolytica. EgDGAT1-1 localized to the endoplasmic reticulum where TAG biosynthesis takes place. Reestablishing neutral lipid accumulation in the Y. lipolytica mutant strain did not induce major reorganization of the yeast microsomal proteome. Overall, our findings demonstrate that EgDGAT1-1 is an endoplasmic reticulum DGAT with preference for medium-chain fatty acid substrates, in line with its physiological role in palm kernel. The characterized EgDGAT1-1 could be used to promote medium-chain triacylglycerol accumulation in microbial-produced oil for industrial chemicals and cosmetics. PMID:26581109

  12. Comparative analysis on the effect of palm oil (Elaeis guineensis in reducing cadmium and lead accumulation in liver of Wistar rats

    Chukwuemeka R Nwokocha

    2012-01-01

    Full Text Available Context : Palm oil from Elaeis guineensis is an edible nutrient substance with anti-inflammatory and antioxidant properties. We examined its protective effect against lead (Pb and cadmium (Cd accumulation in the liver. Materials and Methods : 12% w/w of palm oil (PO in rat chow concentrate was fed to rats exposed to Cd (200ppm and Pb (100ppm in drinking water at different feeding regimens. PO was administered either at the same time with the metals (group 2, post-treatment after exposure (group 3 or pre-treatment before exposure (group 4 for six weeks. The heavy metal accumulations in the liver were determined using AAS. Results : Weight losses induced by these metals were significantly (P<0.05 reversed by PO administration. Analysis among the groups showed that post-treatment group had a significant (P<0.05 higher percentage protection to Cd, but same time treatment for Pb (P<0.05 when compared with other groups. The protective ability to PO was only significantly (P<0.05 increased for Pb at week 2, but showed a time-dependent significant (P<0.05 increase for Cd across all treatment regimens. Conclusion : PO is beneficial in reducing metal accumulation in the liver and has a higher hepatoprotective effect to Cd compared to Pb at the selected doses by possibly affecting the processes of uptake, assimilation and elimination of these metals.

  13. Supplémentation de feuilles de palmier (Elaeis guineensis traitées à la vapeur à des agneaux en Malaisie

    Bengaly, K.

    2015-01-01

    Full Text Available Supplementation of Steamprocessed Oil Palm (Elaeis guineensis Fronds for Lambs in Malaysia. The effect of supplementation of steam-treated oil palm fronds (SOPF at an optimum rate of urea inclusion (16 g/kg together with graded levels of pelleted cassava foliage (PCF on nitrogen balance in lambs was investigated. The PCF was fed in amounts equivalent to 0% (PCF0, 20% (PCF20 or 40% (PCF40 of dry matter (DM intake of steam-treated oil palm fronds to 9 lambs in a completely randomized block design. The level of PCF supplementation had a positive effect (P0.05. Nitrogen balance was only positive when the lambs received the highest level of PCF supplementation (PCF40. Results of rumen metabolites (pH, ammonia-nitrogen and volatile fatty acids did not indicate any depressive effect on microbial digestion of SOPF. It was concluded that SOPF supplemented with adequate amount of urea (i.e. 16 g/kg and small quantities of PCF (e.g. 200 g/kg DM would significantly increase total DM intake without any depressive effect on intake of basal SOPF. The supplementation improved nitrogen balance and live weight gains of the animals.

  14. Molecular cloning and expression of EgTCTP, encoding a calcium binding protein, enhances the growth of callus in oil palm (Elaeis guineensis, Jacq

    Alisa Nakkaew

    2010-12-01

    Full Text Available The translationally controlled tumor protein (TCTP has now been identified in evolutionarily diverse organisms and isthought to play an important role in cell growth and cell division. We have identified an EgTCTP gene from Elaeis guineensisJacq. It is a putative protein of 168 amino acids with a calculated molecular mass of 19.2 kDa. EgTCTP has a high homology(84% - 91% identity at the amino acid level to other plant TCTPs from Hevea brasiliensis, Arachis hypogaea and Glycinemax. The recombinant EgTCTP protein is a calcium binding protein. Transgenic embryonic calli overexpressing EgTCTP havea faster growth rate than non-transformed and empty vector transformed calli. The results show that the enhancement ofEgTCTP gene expression in oil palm embryogenic calli may result in faster multiplication of the embryogenic calli. EgTCTPacts as another Ca2+-modulated protein that is involved in the cell cycle progression.

  15. Use of metabolic control analysis to give quantitative information on control of lipid biosynthesis in the important oil crop, Elaeis guineensis (oilpalm).

    Ramli, Umi S; Salas, Joaquin J; Quant, Patti A; Harwood, John L

    2009-10-01

    * Oil crops are a very important commodity. Although many genes and enzymes involved in lipid accumulation have been identified, much less is known of regulation of the overall process. To address the latter we have applied metabolic control analysis to lipid synthesis in the important crop, oilpalm (Elaeis guineensis). * Top-down metabolic control analysis (TDCA) was applied to callus cultures capable of accumulating appreciable triacylglycerol. The biosynthetic pathway was divided into two blocks, connected by the intermediate acyl-CoAs. Block A comprised enzymes for fatty acid synthesis and Block B comprised enzymes of lipid assembly. * Double manipulation TDCA used diflufenican and bromooctanoate to inhibit Block A and Block B, respectively, giving Block flux control coefficients of 0.61 and 0.39. Monte Carlo simulations provided extra information from previously-reported single manipulation TDCA data, giving Block flux control coefficients of 0.65 and 0.35 for A and B. * These experiments are the first time that double manipulation TDCA has been applied to lipid biosynthesis in any organism. The data show that approaching two-thirds of the total control of carbon flux to lipids in oilpalm cultures lies with the fatty acid synthesis block of reactions. This quantitative information will assist future, informed, genetic manipulation of oilpalm. PMID:19656305

  16. Integrating mixed-effect models into an architectural plant model to simulate inter- and intra-progeny variability: a case study on oil palm (Elaeis guineensis Jacq.).

    Perez, Raphaël P A; Pallas, Benoît; Le Moguédec, Gilles; Rey, Hervé; Griffon, Sébastien; Caliman, Jean-Pierre; Costes, Evelyne; Dauzat, Jean

    2016-08-01

    Three-dimensional (3D) reconstruction of plants is time-consuming and involves considerable levels of data acquisition. This is possibly one reason why the integration of genetic variability into 3D architectural models has so far been largely overlooked. In this study, an allometry-based approach was developed to account for architectural variability in 3D architectural models of oil palm (Elaeis guineensis Jacq.) as a case study. Allometric relationships were used to model architectural traits from individual leaflets to the entire crown while accounting for ontogenetic and morphogenetic gradients. Inter- and intra-progeny variabilities were evaluated for each trait and mixed-effect models were used to estimate the mean and variance parameters required for complete 3D virtual plants. Significant differences in leaf geometry (petiole length, density of leaflets, and rachis curvature) and leaflet morphology (gradients of leaflet length and width) were detected between and within progenies and were modelled in order to generate populations of plants that were consistent with the observed populations. The application of mixed-effect models on allometric relationships highlighted an interesting trade-off between model accuracy and ease of defining parameters for the 3D reconstruction of plants while at the same time integrating their observed variability. Future research will be dedicated to sensitivity analyses coupling the structural model presented here with a radiative balance model in order to identify the key architectural traits involved in light interception efficiency. PMID:27302128

  17. Chronic Administration of Oil Palm (Elaeis guineensis Leaves Extract Attenuates Hyperglycaemic-Induced Oxidative Stress and Improves Renal Histopathology and Function in Experimental Diabetes

    Varatharajan Rajavel

    2012-01-01

    Full Text Available Oil palm (Elaeis guineensis leaves extract (OPLE has antioxidant properties and because oxidative stress contributes to the pathogenesis of diabetic nephropathy (DN, we tested the hypothesis that OPLE prevents diabetes renal oxidative stress, attenuating injury. Sprague-Dawley rats received OPLE (200 and 500 mg kg−1 for 4 and 12 weeks after diabetes induction (streptozotocin 60 mg kg−1. Blood glucose level, body and kidney weights, urine flow rate (UFR, glomerular filtration rate (GFR, and proteinuria were assessed. Oxidative stress variables such as 8-hydroxy-2′-deoxyguanosine (8-OHdG, glutathione (GSH, and lipid peroxides (LPO were quantified. Renal morphology was analysed, and plasma transforming growth factor-beta1 (TGF-β1 was measured. Diabetic rats demonstrated increase in blood glucose and decreased body and increased kidney weights. Renal dysfunction (proteinuria, elevations in UFR and GFR was observed in association with increases in LPO, 8-OHdG, and TGF-β1 and a decrease in GSH. Histological evaluation of diabetic kidney demonstrated glomerulosclerosis and tubulointerstitial fibrosis. OPLE attenuated renal dysfunction, improved oxidative stress markers, and reduced renal pathology in diabetic animals. These results suggest OPLE improves renal dysfunction and pathology in diabetes by reducing oxidative stress; furthermore, the protective effect of OPLE against renal damage in diabetes depends on the dose of OPLE as well as progression of DN.

  18. Protective Effects of Rooibos (Aspalathus linearis and/or Red Palm Oil (Elaeis guineensis Supplementation on tert-Butyl Hydroperoxide-Induced Oxidative Hepatotoxicity in Wistar Rats

    Olawale R. Ajuwon

    2013-01-01

    Full Text Available The possible protective effects of an aqueous rooibos extract (Aspalathus linearis, red palm oil (RPO (Elaeis guineensis, or their combination on tert-butyl-hydroperoxide-(t-BHP-induced oxidative hepatotoxicity in Wistar rats were investigated. tert-butyl hydroperoxide caused a significant (P<0.05 elevation in conjugated dienes (CD and malondialdehyde (MDA levels, significantly (P<0.05 decreased reduced glutathione (GSH and GSH : GSSG ratio, and induced varying changes in activities of catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase in the blood and liver. This apparent oxidative injury was associated with histopathological changes in liver architecture and elevated levels of serum alanine aminotransferase (ALT, aspartate aminotransferase (AST, and lactate dehydrogenase (LDH. Supplementation with rooibos, RPO, or their combination significantly (P<0.05 decreased CD and MDA levels in the liver and reduced serum level of ALT, AST, and LDH. Likewise, changes observed in the activities of antioxidant enzymes and impairment in redox status in the erythrocytes and liver were reversed. The observed protective effects when rooibos and RPO were supplemented concomitantly were neither additive nor synergistic. Our results suggested that rooibos and RPO, either supplemented alone or combined, are capable of alleviating t-BHP-induced oxidative hepatotoxicity, and the mechanism of this protection may involve inhibition of lipid peroxidation and modulation of antioxidants enzymes and glutathione status.

  19. Molecular cloning and characterization of the late embryogenesis abundant group 4 (EgLEA4 gene from oil palm (Elaeis guineensis Jacq

    Watcharasuda Hualkasin

    2013-06-01

    Full Text Available The Late-Embryogenesis Abundant group 4 (LEA4 genes is a group of genes that have been reported to be involved in stress and hormone responses. The completed LEA4 cDNA sequence was first obtained from a set of EST sequences of oil palm (Elaeis guineensis Jacq, named as EgLEA4. The open reading frame is 486 bp in length, encoding a deduced amino acid sequence of 161 residues with a molecular weight of 16.5 kDa and a pI value of about 8.0. Five amino acid motif patterns were found in the EgLEA4 (II, I, III, IV and V and each had a close identity to similar LEA4 patterns of soybean (64%.Comparison of the nucleotide sequences of the cDNA and the genomic DNA demonstrated that the EgLEA4 gene is composed of 2 exons and 1 intron. The 52 untranslated region shows a putative promoter sequence involved in the transcription process, drought stress and hormone responsive elements. RT-PCR analysis showed that the EgLEA4 gene was only expressed in mesocarp, during the late stages of fruit development. It also had a higher expression in induced drought conditions indicating that the EgLEA4 protein may be involved in plant adaptation and stress (drought responsive pathway.

  20. Weed Species Distribution of Juvenile Oil Palm Tree (Elaeis guineensis Intercropped with Maize (Zea mays, Okra (Abelmoshus esculentus and Pepper (Capsicum anuum var. abbreviatum

    Ayodele Samuel OLUWATOBI

    2014-12-01

    Full Text Available This field experiment was carried out to evaluate the weed species distribution in the experimental plots of an intercrop of juvenile oil palm trees (Elaeis guineensis Jacq. with maize (Zea mays Linn., okra (Abelmoschus esculentus Moench and pepper (Capsicum annuum var. abbreviatum. This was carried out during the cropping season between July and October 2012. The crops were intercropped with the juvenile oil palm trees of about 3-years-old. The experiment was laid out in a completely randomized block design with five (5 replicates. The treatments comprised of intercropping distances of 1 m, 2 m and 3 m each for the three (3 crops (maize, okra and pepper and a plot for each of the three (3 arable crops without oil palm trees as control. Weed species distribution was carried out in each of the plots to determine the Simpson’s Diversity Index (D, Simpson’s Index of Diversity (1-D and Simpson’s Reciprocal Index (1/D. Weed species’ frequency, density, relative density, relative frequency, importance value, abundance, dominance and relative dominance were also computed from data collected at 3WAP and 6WAP. The results showed that the control plot has the highest weed species distribution at 3WAP having the lowest Simpson’s Diversity Index (D of 0.0930. Okra plot has the least weed species distribution with the highest Simpson’s Diversity Index (D of 0.2726. At 6WAP, the pepper plot has the highest weed species distribution having the lowest Simpson’s Diversity Index (D of 0.1741. Control plot has the least weed species distribution with highest Simpson’s Diversity Index (D of 0.2831.

  1. EgRBP42 encoding an hnRNP-like RNA-binding protein from Elaeis guineensis Jacq. is responsive to abiotic stresses.

    Yeap, Wan-Chin; Ooi, Tony Eng Keong; Namasivayam, Parameswari; Kulaveerasingam, Harikrishna; Ho, Chai-Ling

    2012-10-01

    RNA-binding proteins (RBPs) have been implicated as regulatory proteins involved in the post-transcriptional processes of gene expression in plants under various stress conditions. In this study, we report the cloning and characterization of a gene, designated as EgRBP42, encoding a member of the plant heterogeneous nuclear ribonucleoprotein (hnRNP)-like RBP family from oil palm (Elaeis guineensis Jacq.). EgRBP42 consists of two N-terminal RNA recognition motifs and a glycine-rich domain at the C-terminus. The upstream region of EgRBP42 has multiple light-responsive, stress-responsive regulatory elements and regulatory elements associated with flower development. Real-time RT-PCR analysis of EgRBP42 showed that EgRBP42 was expressed in oil palm tissues tested, including leaf, shoot apical meristem, root, female inflorescence, male inflorescence and mesocarp with the lowest transcript level in the roots. EgRBP42 protein interacted with transcripts associated with transcription, translation and stress responses using pull-down assay and electrophoretic mobility shift assay. The accumulation of EgRBP42 and its interacting transcripts were induced by abiotic stresses, including salinity, drought, submergence, cold and heat stresses in leaf discs. Collectively, the data suggested that EgRBP42 is a RBP, which responds to various abiotic stresses and could be advantageous for oil palm under stress conditions. Key message EgRBP42 may be involved in the post-transcriptional regulation of stress-related genes important for plant stress response and adaptation. PMID:22699852

  2. SSR markers in transcripts of genes linked to post-transcriptional and transcriptional regulatory functions during vegetative and reproductive development of Elaeis guineensis

    Tranbarger Timothy

    2012-01-01

    Full Text Available Abstract Background The oil palm (Elaeis guineensis Jacq. is a perennial monocotyledonous tropical crop species that is now the world's number one source of edible vegetable oil, and the richest dietary source of provitamin A. While new elite genotypes from traditional breeding programs provide steady yield increases, the long selection cycle (10-12 years and the large areas required to cultivate oil palm make genetic improvement slow and labor intensive. Molecular breeding programs have the potential to make significant impacts on the rate of genetic improvement but the limited molecular resources, in particular the lack of molecular markers for agronomic traits of interest, restrict the application of molecular breeding schemes for oil palm. Results In the current study, 6,103 non-redundant ESTs derived from cDNA libraries of developing vegetative and reproductive tissues were annotated and searched for simple sequence repeats (SSRs. Primer pairs from sequences flanking 289 EST-SSRs were tested to detect polymorphisms in elite breeding parents and their crosses. 230 of these amplified PCR products, 88 of which were polymorphic within the breeding material tested. A detailed analysis and annotation of the EST-SSRs revealed the locations of the polymorphisms within the transcripts, and that the main functional category was related to transcription and post-transcriptional regulation. Indeed, SSR polymorphisms were found in sequences encoding AP2-like, bZIP, zinc finger, MADS-box, and NAC-like transcription factors in addition to other transcriptional regulatory proteins and several RNA interacting proteins. Conclusions The identification of new EST-SSRs that detect polymorphisms in elite breeding material provides tools for molecular breeding strategies. The identification of SSRs within transcripts, in particular those that encode proteins involved in transcriptional and post-transcriptional regulation, will allow insight into the functional

  3. MICORRIZA ARBUSCULAR Y SU EFECTO EN PLÁNTULAS DE Elaeis guineensis (Palmaceae CON ALTO NIVEL DE FÓSFORO EN EL SUELO

    Barrera Berdugo Silvia Eugenia

    2010-04-01

    Full Text Available

    Los hongos micorrizicos arbusculares (HMA facilitan la absorción de nutrientes a las plantas hospederas, por esta razón estos microorganismos cumplen un rol fundamental en el funcionamiento de los agroecosistemas. Con el fin de evaluar el efecto que tienen los HMA nativos y foráneos en Elaeis guineensis se realizó este experimento. Plantas de tres meses de edad fueron sometidas a cuatro tratamientos: IN, IF, Mixto y Testigo. Se evaluó peso seco total de la planta, peso seco raíz, peso seco parte aérea de la planta, altura de la planta, tasa de crecimiento relativo, porcentaje de colonización y número de esporas. Se hicieron tres muestreos, uno inicial, uno a los 45 días y otro a los 90 días. Los datos fueron analizados mediante un ANOVA o Kruskall-Wallis según el comportamiento de los datos, seguido de un test de medias de Duncan o un test modificado de Tuckey para datos no paramétricos. Se presentaron diferencias significativas en el número de esporas entre los tratamientos IN, IF, Mixto y el Testigo y entre IN y Mixto a los 45 días. En el porcentaje de colonización se observaron diferencias entre los tratamientos IF y Testigo a los 45 días. A los 90 días entre todos los tratamientos con respecto al testigo. A pesar de que el nivel de P en el suelo fue alto, también lo fue el porcentaje de colonización en las dos épocas evaluadas. A través del tiempo el tratamiento IN funcionó mejor en las

  4. Réaction de deux espèces d’igname (Dioscorea spp. traitées avec du vin de palme (Elaeis guineensis Jacq., aux champignons responsables des pourritures d’igname

    A. Tschannen

    2009-01-01

    Full Text Available The sensitivity of Dioscorea alata var. bete-bete and D. cayenensis-rotundata var. krengle treated with palm wine obtained from oil palm trees (Elaeis guineensis Jacq. to seven fungi isolated from yam rots was assessed. Yam tubers were collected from two markets in Abidjan and also from storage sites in Bringakro (at 180 km from Abidjan, Ivory Coast. Pathogenecity tests showed that Penicillium oxalicum was the most pathogenic among all fungi isolated. Krengle was more susceptible than bete-bete. In vitro, fermented palm wine totally inhibited mycelial growth of all fungi isolated with the exception of P. oxalicum and Aspergillus niger. Inhibition percentage of mycelial growth of all fungi was more important for krengle than bete-bete. Regardless the yam species, in vivo tests showed that fermented palm wine was more effective than non fermented palm wines when used as a preventive treatment.

  5. Weed Species Distribution of Juvenile Oil Palm Tree (Elaeis guineensis) Intercropped with Maize (Zea mays), Okra (Abelmoshus esculentus) and Pepper (Capsicum anuum var. abbreviatum)

    Ayodele Samuel OLUWATOBI; Kehinde Stephen OLORUNMAIYE

    2014-01-01

    This field experiment was carried out to evaluate the weed species distribution in the experimental plots of an intercrop of juvenile oil palm trees (Elaeis guineensis Jacq.) with maize (Zea mays Linn.), okra (Abelmoschus esculentus Moench) and pepper (Capsicum annuum var. abbreviatum). This was carried out during the cropping season between July and October 2012. The crops were intercropped with the juvenile oil palm trees of about 3-years-old. The experiment was laid out in a completely ran...

  6. Comparison of the effect of two excipients (karite nut butter and vaseline) on the efficacy of Cocos nucifera, Elaeis guineensis and Carapa procera oil-based repellents formulations against mosquitoes biting in Ivory Coast.

    Konan, Y L; Sylla, M S; Doannio, J M; Traoré, S

    2003-06-01

    Repellents in the form of dermal pomades are recommended as a protection against awakening and bedtime mosquito bites. If synthesis repellents are available, they are nevertheless not common and the prices remain out of reach for the communities concerned. The people therefore have to resort more and more to traditional concoctions, some of which have been shown to be effective. After demonstrating that oil-based formulations (lotions, creams, pomades) of Cocos nucifera (coconut), Elaeis guineensis (oil palm) and Carapa procera (gobi) were effective against mosquitoes, it became necessary to study the impact of the two excipients used in their manufacture, on the effectiveness of the repellents. Experiments were carried with Anopheles gambiae and Aedes aegypti under lobaratory conditions and any other mosquitoes collected under field conditions in Ivory Coast. The laboratory results indicate that the average protection times obtained with formulations with karite nut butter as excipient (54.8 +/- 37.0 mn and 74.6 +/- 26.4 mn respectively on An. gambiae and Ae. aegypti) are higher than those recorded with vaseline as excipient (respectively 42.7 +/- 30.0 mn and 60.8 +/- 33.9 mn). On the other hand, under field conditions, the biting rate percentage reduction obtained with the products with karite nut butter and vaseline excipient were similar (respectively 29.8% and 35.9% for all mosquitoes collected and 45.7% and 47.4% against An. gambiae). Nevertheless, the use of karite nut butter on repellent products should be encouraged because its sale price is very lower (10 time less) than the vaseline's. PMID:12847928

  7. Comparison of the effect of two excipients (karite nut butter and vaseline on the efficacy of Cocos nucifera, Elaeis guineensis and Carapa procera oil-based repellents formulations against mosquitoes biting in Ivory Coast

    Konan Y.L.

    2003-06-01

    Full Text Available Repellents in the form of dermal pomades are recommended as a protection against awakening and bedtime mosquito bites. If synthesis repellents are available, they are nevertheless not common and the prices remain out of reach for the communities concerned. The people therefore have to resort more and more to traditional concoctions, some of which have been shown to be effective. After demonstrating that oil-based formulations (lotions, creams, pomades of Cocos nucifera (coconut, Elaeis guineensis (oil palm and Carapa procera (gobi were effective against mosquitoes, it became necessary to study the impact of the two excipients used in their manufacture, on the effectiveness of the repellents. Experiments were carried with Anopheles gambiae and Aedes aegypti under lobaratory conditions and any other mosquitoes collected under field conditions in Ivory Coast. The laboratory results indicate that the average protection times obtained with formulations with karite nut butter as excipient (54.8 ± 37.0 mn and 74.6 ± 26.4 mn respectively on An. gambiae and Ae. aegypti are higher than those recorded with vaseline as excipient (respectively 42.7 ± 30.0 mn and 60.8 ± 33.9 mn. On the other hand, under field conditions, the biting rate percentage reduction obtained with the products with karite nut butter and vaseline excipient were similar (respectively 29.8 % and 35.9 % for all mosquitoes collected and 45.7 % and 47.4 % against An. gambiae. Nevertheless, the use of karite nut butter on repellent products should be encouraged because its sale price is very lower (10 time less than the vaseline's.

  8. Expression profiles of defence related cDNAs in oil palm (Elaeis guineensis Jacq.) inoculated with mycorrhizae and Trichoderma harzianum Rifai T32.

    Tan, Yung-Chie; Wong, Mui-Yun; Ho, Chai-Ling

    2015-11-01

    Basal stem rot is one of the major diseases of oil palm (Elaies guineensis Jacq.) caused by pathogenic Ganoderma species. Trichoderma and mycorrhizae were proposed to be able to reduce the disease severity. However, their roles in improving oil palm defence system by possibly inducing defence-related genes in the host are not well characterized. To better understand that, transcript profiles of eleven putative defence-related cDNAs in the roots of oil palm inoculated with Trichoderma harzianum T32 and mycorrhizae at different time points were studied. Transcripts encoding putative Bowman-Birk protease inhibitor (EgBBI2) and defensin (EgDFS) increased more than 2 fold in mycorrhizae-treated roots at 6 weeks post inoculation (wpi) compared to those in controls. Transcripts encoding putative dehydrin (EgDHN), glycine-rich RNA binding protein (EgGRRBP), isoflavone reductase (EgIFR), type 2 ribosome inactivating protein (EgT2RIP), and EgDFS increased in the oil palm roots treated with T. harzianum at 6 and/or 12 wpi compared to those in the controls. Some of these genes were also expressed in oil palm roots treated with Ganoderma boninense. This study provides an insight of some defence-related genes induced by Trichoderma and mycorrhizae, and their roles as potential agents to boost the plant defence system. PMID:26322853

  9. Dynamic Expression Analysis of miRNAs during the Development Process of Oil Palm Mesocarp%油棕(Elaeis guineensis)中果皮发育过程中miRNA的表达动态分析

    方良; 梁远学; 李东栋; 曹献英; 郑育声

    2013-01-01

    We obtained high quantity small RNA from the mesocarp of oil palm (Elaeis guineensis) and screened fatty acid biosynthesis related miRNAs.The extracted method of CTAB was improved and then employed to isolate small RNA from the mesocarp of oil palm nuts at five development stages (G1-G5).Twelve miRNAs were screened from our previous database of oil palm small RNA using bioinformatics analysis.Relative expression of each miRNA was determined by stem-loop real-time quantitative PCR(qPCR) and further predicted the targets of these miRNAs.The results indicated that the OD260/OD280 values of the small RNA were between 1.7 and 2.0,and the concentrations of G1 to G5 were 289 ng/μL,364 ng/μL,476 ng/μL,213 ng/μL,and 390 ng/μL,respectively.Relative expression detected by qPCR showed that the twelve miRNAs exhibited significantly different expression during the five development stages,especially high expression level in the fourth stage (G4) and fifth stage (G5).Among them,miR395 and miR156 were highest in the G4 phase and miR395 and miR528 were highest in the G5 phase.Target prediction suggested that some targets of these differently expressed miRNAs were involved in the fatty acid metabolism pathway,such as phosphatidate,phosphatase and phospholipase D.In this study,high quantity small RNA was isolated by the improved CTAB method.qPCR further determined that the twelve candidate miRNAs were differently expressed during the five development stages,of which five miRNAs may be involved in the regulatory pathway of fatty acid metabolism.%以CTAB法提取油棕(Elaeis guineensis)中果皮5个不同发育时期(G1~G5)的小RNA.从前期研究获得的油棕小RNA测序数据库中筛选12个候选miRNA,实时荧光定量PCR法(qRT-PCR)检测其在果实发育过程中的表达量变化,并进一步对显著差异表达的miRNA进行靶基因预测.结果表明:中果皮5个不同发育时期小RNA的OD260/OD280比值在1.7~2.0之间;浓度分别是289、364、476、213

  10. Survival of Coelaenomenodera lameensis (Coleoptera: Chrysomelidae) in Relation to the Physical Characteristics of Different Oil Palm (Elaeis sp.) Breeding Populations

    Beaudoin-Ollivier, L.; Flori, A.; Coffi, A.; Cros, D.; Glitho, I.; Nodichao, L.

    2015-01-01

    The edibility of different Elaeis sp. breeding populations present in Benin was tested for the leaf miner Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), a major oil palm pest in Africa. Experiments carried out in sleeves revealed the oviposition capacities of females and the mortality rates for the different developmental stages by comparing the populations found on two breeding populations of Elaeis oleifera (HBK) Cortes, four of Elaeis guineensis Jacquin and four (E. guineens...

  11. Boron toxicity in oil palm (Elaeis guineensis)

    Rajaratnam, J.A.

    1973-01-01

    Potted oil palms were treated with fertilizer of borate-46 at several concentrations and the plants were observed for boron toxicity effects. Toxicity symptoms were apparent at high rates but not at rates equivalent to typical Malaysian soils.

  12. Comparison of the effect of two excipients (karite nut butter and vaseline) on the efficacy of Cocos nucifera, Elaeis guineensis and Carapa procera oil-based repellents formulations against mosquitoes biting in Ivory Coast

    Konan Y.L.; Sylla M.S.; Doannio J.M.C.; Traoré S.

    2003-01-01

    Repellents in the form of dermal pomades are recommended as a protection against awakening and bedtime mosquito bites. If synthesis repellents are available, they are nevertheless not common and the prices remain out of reach for the communities concerned. The people therefore have to resort more and more to traditional concoctions, some of which have been shown to be effective. After demonstrating that oil-based formulations (lotions, creams, pomades) of Cocos nucifera (coconut), Elaeis guin...

  13. Estandarización de la técnica molecular de aflp en palma de aceite tipo dura (elaeis guineensis jacq.) y estudio preliminar de caracterización

    Galeano, Carlos Hernando

    2012-01-01

    La palma de aceite (Elaeis guinnensis Jacq.) desempeña un importante papel en la econo­mía de algunos países tropicales; Colombia ocupa el primer puesto en producción en América Latina y el quinto lugar a nivel mundial. En esta especie el mejo­ramiento genético busca generar materiales con alta producción de aceite y adaptados a las características edafoclimáticas de las diferentes zonas palmeras. El objetivo del presente trabajo fue estandarizar la téc­nica de AFLP (Amplified Fragment-Length...

  14. Uji Kompatibilitas Mikoriza Vesikular Arbuskular Pada Pertumbuhan Bibit Kelapa Sawit (Elaeis guimensis Jacq) di Pembibitan Pada Media Tanam Histosol dan Ultisol

    Fransiska, Eko

    2013-01-01

    A green house research. Vesicular Arbuscular Mychorrizal Compatibility Test of Oil Palm ( Elaeis guineensis Jacq ) Seed Growth at Nursery on Histosol and Ultisol. It was started on June 2004 till July 2005. A research aim to testing the vesicular arbuscular mychorrizal compatibility of oil palm ( Elaeis guineemis Jacq ) seed growth at nursery on histosol and ultisol. Analys was conducted by using the factorial randomized block design in which, 16 treatments and 3 replications. Each trea...

  15. Pengaruh Pemberian Limbah Kalapa sawit (Sludge) dan Pupuk Majemuk NPK Terhadap Pertumbuhan Bibit Kelapa Sawit (Elaeis guinsensis Jacq) di Pembibitan Awal

    Siregar, Zulkarnain

    2012-01-01

    Penelitian bertujuan untuk mengetahui Pengaruh pemberian Limbah Kelapa Sawit (Sludge) dan Pupuk Majemuk NPK terhadap Pertumbuhan Bibit Kelapa Sawit (Elaeis guineensis Jacq) di Pre Nursery. Penelitian ini dilaksanakan di Kelurahan Gedung Johor Kecamatan Medan Johor pada ketinggian 27 meter diatas permukaan laut. Penelitian berlangsung dari bulan November-Maret 2001.

  16. Determination of Antioxidants in Oil Palm Leaves (Elaeis guineensis)

    Ng M. Han; Choo Y. May

    2010-01-01

    Problem statement: Previous findings on the occurrence of water soluble antioxidants in palm oil has brought to the question on whether these compounds is also present in other parts of the oil palm; namely its leaves. Approach: It is now believed that the water soluble antioxidants are also present in other biomass of the oil palm, namely, the leaves. This study reported on the determination of the water soluble antioxidants in oil palm leaves. Results: The results showed the analyses of the...

  17. Milox fractionation of empty fruit bunches from Elaeis guineensis.

    Ferrer, Ana; Vega, Alberto; Rodríguez, Alejandro; Ligero, Pablo; Jiménez, Luis

    2011-10-01

    Milox pulping of EFB was used to obtain pulps. In the first Milox stage, the influences of operating variables on pulp properties were studied and polynomial and neural fuzzy models that reproduced the experimental results with errors less than 10% were developed. Operating variables were found (93 wt.% of formic acid, 3 wt.% of hydrogen peroxide, and 165 min) that yielded acceptable pulp properties (40.5% yield, 50.3% brightness and 608 mL/g viscosity) at reasonable chemical and energy costs. The second stage was studied by subjecting the liquors of the previously optimized first stage to different treatment times. This time should be 30 min or less, to avoid a negative effect on viscosity. The residual liquor from the first Milox stage contained virtually no precipitable lignin and only low amounts of sugar (wt.%): glucose 0.71, xylose 4.22, galactose 1.19, mannose 0.22, all on original raw material dry. PMID:21855324

  18. Survival of Coelaenomenodera lameensis (Coleoptera: Chrysomelidae) in Relation to the Physical Characteristics of Different Oil Palm (Elaeis sp.) Breeding Populations.

    Beaudoin-Ollivier, L; Flori, A; Coffi, A; Cros, D; Glitho, I; Nodichao, L

    2015-01-01

    The edibility of different Elaeis sp. breeding populations present in Benin was tested for the leaf miner Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), a major oil palm pest in Africa. Experiments carried out in sleeves revealed the oviposition capacities of females and the mortality rates for the different developmental stages by comparing the populations found on two breeding populations of Elaeis oleifera (HBK) Cortes, four of Elaeis guineensis Jacquin and four (E. guineensis × E. oleifera) × E. guineensis backcrosses. Females laid their eggs similarly on all breeding populations, with a preference for the E. guineensis La Mé origin. The average hatching rate reached 80% for the La Mé origin as opposed to 28% for the Deli origin. The mortality rates for the larval instars were greater on E. oleifera, on certain backcrosses and on the Deli origin of E. guineensis. Development at the second- and third- larval instars was the most affected, with a mortality rate of three to five times greater than that seen on La Mé. Epidermis and cuticle measurements indicated which breeding populations were suitable or unsuitable for the development of C. lameensis. E. guineensis, with its thin epidermis (12 µm) and cuticle (2 µm), proved to be highly susceptible to C. lameensis attacks. On the other hand, E. oleifera, which is very resistant, exhibited a thicker epidermis (17 µm) and cuticle (4 µm). The breeding populations were thus classified according to the positive or negative influence they exerted on the insect's egg laying and feeding. PMID:26113512

  19. Elaeis oleifera genomic-SSR markers: exploitation in oil palm germplasm diversity and cross-amplification in arecaceae.

    Zaki, Noorhariza Mohd; Singh, Rajinder; Rosli, Rozana; Ismail, Ismanizan

    2012-01-01

    Species-specific simple sequence repeat (SSR) markers are favored for genetic studies and marker-assisted selection (MAS) breeding for oil palm genetic improvement. This report characterizes 20 SSR markers from an Elaeis oleifera genomic library (gSSR). Characterization of the repeat type in 2000 sequences revealed a high percentage of di-nucleotides (63.6%), followed by tri-nucleotides (24.2%). Primer pairs were successfully designed for 394 of the E. oleifera gSSRs. Subsequent analysis showed the ability of the 20 selected E. oleifera gSSR markers to reveal genetic diversity in the genus Elaeis. The average Polymorphism Information Content (PIC) value for the SSRs was 0.402, with the tri-repeats showing the highest average PIC (0.626). Low values of observed heterozygosity (H(o)) (0.164) and highly positive fixation indices (F(is)) in the E. oleifera germplasm collection, compared to the E. guineensis, indicated an excess of homozygosity in E. oleifera. The transferability of the markers to closely related palms, Elaeis guineensis, Cocos nucifera and ornamental palms is also reported. Sequencing the amplicons of three selected E. oleifera gSSRs across both species and palm taxa revealed variations in the repeat-units. The study showed the potential of E. oleifera gSSR markers to reveal genetic diversity in the genus Elaeis. The markers are also a valuable genetic resource for studying E. oleifera and other genus in the Arecaceae family. PMID:22605966

  20. Elaeis oleifera Genomic-SSR Markers: Exploitation in Oil Palm Germplasm Diversity and Cross-Amplification in Arecaceae

    Ismanizan Ismail

    2012-03-01

    Full Text Available Species-specific simple sequence repeat (SSR markers are favored for genetic studies and marker-assisted selection (MAS breeding for oil palm genetic improvement. This report characterizes 20 SSR markers from an Elaeis oleifera genomic library (gSSR. Characterization of the repeat type in 2000 sequences revealed a high percentage of di-nucleotides (63.6%, followed by tri-nucleotides (24.2%. Primer pairs were successfully designed for 394 of the E. oleifera gSSRs. Subsequent analysis showed the ability of the 20 selected E. oleifera gSSR markers to reveal genetic diversity in the genus Elaeis. The average Polymorphism Information Content (PIC value for the SSRs was 0.402, with the tri-repeats showing the highest average PIC (0.626. Low values of observed heterozygosity (Ho (0.164 and highly positive fixation indices (Fis in the E. oleifera germplasm collection, compared to the E. guineensis, indicated an excess of homozygosity in E. oleifera. The transferability of the markers to closely related palms, Elaeis guineensis, Cocos nucifera and ornamental palms is also reported. Sequencing the amplicons of three selected E. oleifera gSSRs across both species and palm taxa revealed variations in the repeat-units. The study showed the potential of E. oleifera gSSR markers to reveal genetic diversity in the genus Elaeis. The markers are also a valuable genetic resource for studying E. oleifera and other genus in the Arecaceae family.

  1. Analysis of beta-carotene hydroxylase gene cDNA isolated from the American oil-palm (Elaeis oleifera) mesocarp tissue cDNA library

    Bhore, Subhash J.; Kassim, Amelia; Loh, Chye Ying; Shah, Farida H

    2010-01-01

    It is well known that the nutritional quality of the American oil-palm (Elaeis oleifera) mesocarp oil is superior to that of African oil-palm (Elaeis guineensis Jacq. Tenera) mesocarp oil. Therefore, it is of important to identify the genetic features for its superior value. This could be achieved through the genome sequencing of the oil-palm. However, the genome sequence is not available in the public domain due to commercial secrecy. Hence, we constructed a cDNA library and generated expres...

  2. Development and maturation of fruits of two indupalma oxg hybrids (elaeis oleifera x elaeis guineensis)

    Diego A González G; Gerardo Cayón S.; Jesús E López M; Wilmar H Alarcón

    2013-01-01

    OxG hybrids are materials with characteristics notable for tolerance to pests and diseases, high quality oil and acceptable bunch production, but the physiological processes of growth and maturation of the fruits are not well known. For the two hybrid materials (Coarí x La Mé and Sinú-Coarí x La Mé), the physiological and biochemical changes during the growth and maturation of the bunches were studied in San Alberto, Cesar (Colombia) with a climate of 2,497 mm/year precipitation and 27°C. Fem...

  3. Survival of Coelaenomenodera lameensis (Coleoptera: Chrysomelidae) in Relation to the Physical Characteristics of Different Oil Palm (Elaeis sp.) Breeding Populations

    Beaudoin-Ollivier, L.; Flori, A.; Coffi, A.; Cros, D.; Glitho, I.; Nodichao, L.

    2015-01-01

    The edibility of different Elaeis sp. breeding populations present in Benin was tested for the leaf miner Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), a major oil palm pest in Africa. Experiments carried out in sleeves revealed the oviposition capacities of females and the mortality rates for the different developmental stages by comparing the populations found on two breeding populations of Elaeis oleifera (HBK) Cortes, four of Elaeis guineensis Jacquin and four (E. guineensis × E. oleifera) × E. guineensis backcrosses. Females laid their eggs similarly on all breeding populations, with a preference for the E. guineensis La Mé origin. The average hatching rate reached 80% for the La Mé origin as opposed to 28% for the Deli origin. The mortality rates for the larval instars were greater on E. oleifera, on certain backcrosses and on the Deli origin of E. guineensis. Development at the second- and third- larval instars was the most affected, with a mortality rate of three to five times greater than that seen on La Mé. Epidermis and cuticle measurements indicated which breeding populations were suitable or unsuitable for the development of C. lameensis. E. guineensis, with its thin epidermis (12 µm) and cuticle (2 µm), proved to be highly susceptible to C. lameensis attacks. On the other hand, E. oleifera, which is very resistant, exhibited a thicker epidermis (17 µm) and cuticle (4 µm). The breeding populations were thus classified according to the positive or negative influence they exerted on the insect’s egg laying and feeding. RÉSUMÉ. La comestibilité de différents matériels végétaux d’Elaeis sp. présents au Bénin est testée pour Coelaenomenodera lameensis Berti (Coleoptera: Chrysomelidae), important ravageur du palmier à huile en Afrique. Des expérimentations en manchons ont permis de déterminer les capacités d’oviposition des femelles et les taux de mortalité des différents stades de d

  4. Palm (Elaeis guineensis L. kernel cake in diets for dairy cowsTorta de dendê (Elaeis guineensis L. em dietas de vacas leiteiras

    Odislei Fagner Ribeiro Cunha

    2013-03-01

    Full Text Available The objective of the present study was to assess the effect of different dietary inclusion levels of palm (Elaeisguineensis L. kernel cake (PKC for lactating dairy cows on feed intake, nutrient digestibility and milk production. Diets containing 0, 113, 228, 342g of PKC/kg dry matter were formulated and fed to eight crossbred (Holandês x Zebu dairy cows with mean weight of 382kg at 60-90 days of lactation. The cows were used in a double 4 x 4 Latin square design. Each experimental period lasted for 15 days, with 11 days adaptation and four days sampling. Increasing the levels of PKC in the diet reduced the intake of dry matter (DM, crude protein (CP, hemicellulose, non-fibrous carbohydrates (NFC and total digestible nutrients (TDN. The NDF intake was higher at the 113g/kg PKC inclusion level compared to other treatments. There was linear decreasing effect on fat corrected milk with the inclusion of PKC. There was linear reduction in digestibility of dry matter(DM and total carbohydrate (TC, but no effect was observed on the NDF and ADF digestibility. Linear increase was observed on crude protein (CP, NFC and ether extract (EE digestibility. The digestibility of these nutrients probably increased because of the longer retention time of the digest in the rumen caused by reduced DM intake. The addition of PKC decreased the nutritive value of the diets, which subsequently reduced linearly milk production.O objetivo do presente trabalho foi avaliar o efeito da inclusão (0.0; 113,4; 227,8 e 341,7 g/kg de matéria seca da torta de dendê (TD em dietas para vacas leiteiras lactantes sobre o consumo, digestibilidade aparente da matéria seca e dos nutrientes e produção de leite. Foram utilizadas oito vacas mestiças Holandês x Zebu, primíparas, com peso médio de 382 kg, entre 60 e 90 dias de lactação, distribuídas em duplos quadrados latinos 4 x 4. Cada período experimental teve duração de 15 dias, sendo 11 dias para adaptação e quatro para coletas. Avaliou- se o consumo, expresso em kg/dia, g/kgpv0,75 e g/kg PV, a digestibilidade aparente da matéria seca ( MS, proteina bruta (PB, fibra em detergente neutro (FDN, fibra em detergente ácido (FDA, hemicelulose (HEM, carboidratos não fibrosos (CNF, extrato etéreo (EE e nutrientes digestíveis totais (NDT e a produção de leite corrigida para gordura (PLCG. A inclusão de níveis crescentes de TD na dieta acarretou redução linear no consumo de MS, PB, HEM, CNF e NDT. O consumo de FDN apresentou efeito quadrático e os maiores valores foram observados com o nível de inclusão de TD de 113,4 g/kg MS. Houve efeito linear decrescente sobre a PLCG com a inclusão da TD. Houve redução linear da digestibilidade da MS e dos CT, porém não se observou efeito sobre a digestibilidade da FDN e FDA. Observou-se ainda aumento linear da digestibilidade da PB, CNF e EE. O aumento da digestibilidade destes nutrientes provavelmente ocorreu devido ao maior tempo de retenção da digesta no rúmen ocasionado pela redução no consumo de MS. A adição de TD diminui o valor nutritivo das dietas reduzindo linearmente a produção de leite.

  5. mRNA Expression of EgCHI1, EgCHI2, and EgCHI3 in Oil Palm Leaves (Elaeis guineesis Jacq.) after Treatment with Ganoderma boninense Pat. and Trichoderma harzianum Rifai

    Laila Naher; Soon Guan Tan; Chai Ling Ho; Umi Kalsom Yusuf; Siti Hazar Ahmad; Faridah Abdullah

    2012-01-01

    Background. Basal stem rot (BSR) disease caused by the fungus Ganoderma boninense is the most serious disease affecting the oil palm; this is because the disease escapes the early disease detection. The biocontrol agent Trichoderma harzianum can protect the disease only at the early stage of the disease. In the present study, the expression levels of three oil palm (Elaeis guineensis Jacq.) chitinases encoding EgCHI1, EgCHI2, and EgCHI3 at 2, 5, and 8 weeks inoculation were measured in oil pa...

  6. Estudio de las variables de proceso mediante superficie de respuesta para la optimización de la producción de biodiesel utilizando aceite de palma africana (Elaeis guineenis Jacq) como materia prima.

    Anguebes Franseschi, Francisco

    2014-01-01

    El aceite de palma africana (Elaeis guineensis Jacq) representa una opción atractiva para emplearse como materia prima en la fabricación de biodiesel. El Objetivo de este trabajo fue maximizar el rendimiento de biodiesel sintetizado a partir de oleína cruda de palma africana en el laboratorio. La síntesis de biodiesel se realizó en dos etapas: a) una esterificación ácida realizada con ácido sulfúrico y b) una transesterificación alcalina empleando hidróxido de sodio. Para hallar las condicion...

  7. Production of biodiesel from palm oil (Elaeis guineensis) using heterogeneous catalyst: An optimized process

    Hameed, B.H.; Lai, L.F.; Chin, L.H. [School of Chemical Engineering, Engineering Campus, University of Science Malaysia, 14300 Nibong Tebal, Penang (Malaysia)

    2009-04-15

    Response surface methodology (RSM) based on central composite design (CCD) was used to optimize the three important reaction variables - methanol/oil molar ratio (x{sub 1}), reaction time (x{sub 2}) and amount of catalyst (x{sub 3}) for production of biodiesel from palm oil using KF/ZnO catalyst. Based on the CCD, a quadratic model was developed to correlate the reaction variables to the biodiesel yield. From the analysis of variance (ANOVA), the most influential factor on the experimental design response was identified. The predicted yield after process optimization was found to agree satisfactory with the experimental value. The optimum conditions for biodiesel production were found as follows: methanol/oil ratio of 11.43, reaction time of 9.72 h and catalyst amount of 5.52 wt%. The optimum biodiesel yield was 89.23%. (author)

  8. Metabolismo de carbohidratos en palmas de aceite (elaeis guineensis jacq.) afectadas por marchitez letal

    Pèrez Angela Patricia; Cayòn Gerardo

    2010-01-01

    La enfermedad conocida como “marchitez letal” se presenta
    en plantaciones de la zona baja del río Upía, en palmas de siete
    años de sembradas. No se conoce el posible agente causal o
    factores que predisponen a las palmas a la enfermedad, y no se
    ha registrado hasta la fecha en otras zonas palmeras del país. Se
    analizó el metabolismo de carbohidratos en palmas de aceite
    con síntomas iniciales de la enfermedad, palmas sospechosas&...

  9. Protein Profiling and Histone Deacetylation Activities in Somaclonal Variants of Oil Palm (Elaeis guineensis Jacq.)

    Jamilah Syafawati Yaacob; Hwei-San Loh; Rosna Mat Taha

    2013-01-01

    Mantled fruits as a result of somaclonal variation are often observed from the oil palm plantlets regenerated via tissue culture. The mantling of fruits with finger-like and thick outer coating phenotypes significantly reduces the seed size and oil content, posing a threat to oil palm planters, and may jeopardize the economic growth of countries that depend particularly on oil palm plantation. The molecular aspects of the occurrence of somaclonal variations are yet to be known, possibly due t...

  10. The shoot apical meristem of oil palm (Elaeis guineensis; Arecaceae): developmental progression and dynamics

    Jouannic, Stefan; Lartaud, Marc; Hervé, Jonathan; Collin, Myriam; Orieux, Yves; Verdeil, Jean-Luc; Tregear, James W

    2011-01-01

    Background and Aims Oil palm, an unbranched perennial monocotyledon, possesses a single shoot apical meristem (SAM), which is responsible for the initiation of the entire above-ground structure of the plant. To compare the palm SAM structure with those of other monocots and to study variations in its structure throughout the life of the plant, its organization was characterized from the embryonic stage to that of the reproductive plant. Methods SAM structure was studied by a combination of st...

  11. Protein profiling and histone deacetylation activities in somaclonal variants of oil palm (Elaeis guineensis Jacq.).

    Yaacob, Jamilah Syafawati; Loh, Hwei-San; Mat Taha, Rosna

    2013-01-01

    Mantled fruits as a result of somaclonal variation are often observed from the oil palm plantlets regenerated via tissue culture. The mantling of fruits with finger-like and thick outer coating phenotypes significantly reduces the seed size and oil content, posing a threat to oil palm planters, and may jeopardize the economic growth of countries that depend particularly on oil palm plantation. The molecular aspects of the occurrence of somaclonal variations are yet to be known, possibly due to gene repression such as DNA methylation, histone methylation and histone deacetylation. Histone deacetylases (HDACs), involved in eukaryotic gene regulation by catalyzing the acetyl groups are removal from lysine residues on histone, hence transcriptionally repress gene expression. This paper described the total protein polymorphism profiles of somaclonal variants of oil palm and the effects of histone deacetylation on this phenomenon. Parallel to the different phenotypes, the protein polymorphism profiles of the mantled samples (leaves, fruits, and florets) and the phenotypically normal samples were proven to be different. Higher HDAC activity was found in mantled leaf samples than in the phenotypically normal leaf samples, leading to a preliminary conclusion that histone deacetylation suppressed gene expression and contributed to the development of somaclonal variants. PMID:23844406

  12. Towards more efficient selection for oil yield in the oil palm (Elaeis guineensis Jacquin)

    Vossen, van der H.A.M.

    1974-01-01

    Detailed studies are made of the variability and heritability of a number of components of oil yield in the oil palm using published data of the breeding programme of the NIFOR in Nigeria and results of a number of experiments carried out at the OPRC in Ghana during the period 1965-1971. Estimations

  13. Analisis Sifat Fisis dan Aplikasi Penggunaan Fiberbrick dari Serat Tandan Kosong Sawit (Elaeis guineensis Jacq.)

    Ariyandi, Ori

    2011-01-01

    Utilization of oil palm empty fruit bunch fibers as raw material of fiberbrick is not only provides the added value for empty fruit bunches, but also it can be used for subtituting wood material. The purpose of this study is to invertigate the physical properties and application of fiberbrick from empty fruit bunch fibers. Further, the grave yard test and the influence of fiber dimension, concentration of adhesive and fiber mass of fiberbrick were also evaluated. The hot pressure was appli...

  14. Evaluation Of The Radical Activity In African Palm (Elaeis Guineensis JACQ) Using Match-32

    The low, humid and tropical lands, are potential producers of palm of oil, since this plant is notoriously tolerant to the poor floors in nutritious. The palm of oil cultivation is relatively new, commercial seeding hardly dates of 1950 and in spite of this, the futurist vision of some managers they have allowed that this activity, in few years of existence, have had a growing dynamics of the agricultural sector. The objective of this study, was to evaluate under field conditions the radical activity, making an analysis in the efficiency of use of the mono-ammonic phosphate with 32P that it took place in the INEA with a lmCi/gram activity. it is Also to determine the differences of absorption in the leaves 1,9 and 17 following the phylotaxia of the palm leaves, in order to determine the leaf number for the analysis to foliate. The work was carried out in a 6 year-old cultivation belonging to the company Unipalma, located in the municipality of Cumaral, department of the goal, located to 500 msnm, being a representative lot where African palm is cultivated in the oriental plains. I am accustomed to classified as an Oxi sol, with pH of 4.5 and with high iron contents and aluminum. In this work the methodology isotopic was used of tracer. The study checked that the methodology was highly effective, since it was determined that the biggest radical activity is 2.5 m of the foot of the plant, with a percentage average of absorption of 60.8 with regard to the other treatments. Because they were not differences in match absorption among the leaves 1, 9 and 17, it is recommended for the analysis to foliate to make use of the any leaf number. As for the efficiency of use of the mono-ammonic phosphate it was low (2.0710), it becomes necessary to promote studies tendentious to improve their behavior, stiller keeping in mind the high cost of the fertilizers and the risks of ecological contamination

  15. Radical activity evaluation in African Palm (Elaeis guineensis Jacq) using phosphorous - 32

    Low lands, wet and tropical, its are producing potential of oil palm, since this plant is notoriously bearable to poor soils in nutrients. Oil palm cultivation is relatively new at Colombia, commercial sowings date hardly of 1950s and in spite of this, tenacity and futuristic vision of some entrepreneurs have permitted that this activity, in few existence years, may have had a growing dynamics and strengthening of agricultural sector. The objective of this Thesis, it's to evaluate field conditions of radical activity, making an analysis in utilization efficiency of monoammonium phosphate labeled with 32P that was produced at INEA (today Ingeominas) with an activity of 1mCi/gram. Furthermore, its are intended to define differences of absorption in 1,9 and 17 leaves continuing the palm leaves phyto taxis, with the objective to determine the number of leaf for foliate analysis. The work was carried out in a 6-year-old age cultivation belonging to Uni palma company, located at Cumaral Municipality (Meta Department), situated to 500 meters on sea level, being a representative batch of African palm cultivation at Colombia's Eastern Plains. Soil is classified as Oxi sol, with ph of 4.5 and with high iron and aluminum indices. It was used the Isotopic Tracers methodology, the one which was highly effective, inasmuch as was determined that greater radical activity is found 2.5 meters of plant foot, with a percentage absorption average of 60.8 with respect to other treatments. Due to fact that were not found differences in phosphorus absorption between leaves 1,9 and 17, is recommended for foliate analysis to make use of any number of leaf. Efficiency of use of monoammonium phosphate was decreased (2.0710), something which makes necessary to promote tending studies to improve its behavior, more yet taking into account high cost of fertilizers and environmental pollution risks

  16. Respon Morfologi dan Fisiologi Pada Kelapa Sawit (Elaeis guineensis Jacq.) Terhadap Aplikasi Pupuk Magnesium Dan Nitrogen

    Panjaitan, Lily Damery

    2014-01-01

    The aim of the present study is to investigate the physiological and morphological responses to three concentration of magnesium (low,medium and high) and two level nitrogen (with or without) fertilization of oil palm. Factorial Randomized Design was used 2 factors. The oil palm was 8 years old. The treatmenst with nitrogen and magnesium fertilizer has been starting since 2007-2013. Observation was started on July-September 2013 at Physiology laboratory of North Sumtera University. Total samp...

  17. Spectral reflectance of oil-palm (Elaeis guineensis Jacq. bunches as indicator for optimal harvesting

    Wunsri, S.

    2005-12-01

    Full Text Available To determine the appropriate harvesting time of Tenera oil palm bunches, the spectral reflectance of ripening bunches was investigated from images taken by a digital Canon PowerShot A20 camera. Every second day images were taken from selected bunches at an oil-palm estate during the growth of the bunchesfrom immaturity to the over-ripe phase, to trace the changes in color which correlate with the process of ripening. The images were analyzed measuring the changes in the three basic colors red, green and blue, using a specially developed Color Analysis computer program for Oil-palm 'fruit' (CAOP, and determining the correlation with the measured palm-oil content of the nuts.Of the three color-bands, the red light reflectance showed the best correlation with ripeness, as the red color increased regularly and practically linearly up to the stage of the maximal oil yield. Some seven days after the thus determined optimal harvesting date for the ripe bunches, some of the ripe nuts will detachfrom the bunch and fall, but then it is too late to use the fallen nuts as indicators for harvesting. From our investigations in the field and in the laboratory we recommend that harvesting should follow immediately after the red reflectance starts declining, after having reached its peak value for the Tenera oil-palm some 5±3 days before nuts begin to detach from the bunch.

  18. Oil Palm (Elaeis guineensis Roots Response to Mechanization in Bernam Series Soil

    Zuraidah Yahya

    2010-01-01

    Full Text Available Problem statement: Field practices involving the use of mechanization in oil palm plantations could result in soil compaction which alters the soil physical properties. The gradual deterioration of soil physical conditions could restrict the growth and function of roots. This study was carried out to evaluate the response of oil palm roots to changes in soil physical properties due to mechanization in Bernam series soil belonging to the clay texture class. Approach: Compaction treatments were imposed for 6 consecutive years and a comparison was done on the effects of different trailer weights on oil palm roots growth. Roots and soil were sampled using root and soil augers at 0-30 cm depth from the harvesting and frond pile paths. Results: The results showed that the oil palm roots were affected by the mechanization treatments. Growth of oil palm roots was significantly affected by the 4T trailer weight. Palms in compacted soil produced less primary and secondary roots but this was compensated for by the production of longer and thicker tertiary and quaternary roots. Conclusion: The compaction treatments affect the soil physical properties, which in turn affect the growth and distribution of oil palm roots.

  19. Uji Toksisitas Subkronik Ekstrak Etanol Daun Kelapa Sawit (Elaeis guineensis Jacq.) Menggunakan Mencit Jantan

    Wahyuni, Yetri

    2016-01-01

    Medicinal plants have been known and used mostly by people and they are more safe than modern medicine. One of them is palm leaves which is used as an injury healing, antimicrobial, antioxidant, antidiabetic, antihypertensive and hepatoprotector. However, subchronic toxicity of palm leaves haven’tbeen known.The purpose ofthis research wasto determine the safetyofpalm leavesat a certain periode. Simplicia of palm leaves was macerated with ethanol 80% and evaporated using rotary evaporator...

  20. Alterations of palm oil (Elaeis guineensis) in the continuous industrial par frying of breaded chicken snacks

    Débora Rezende Ferreira; Adelaide Del Pino Beléia; Rui Sérgio dos Santos Ferreira da Silva

    2014-01-01

    The physical and chemical alterations in palm oil during continuous industrial par frying of breaded chicken snacks were evaluated using a pseudo first-order kinetic model. The acidity index, refractive index, concentration of polar compounds, viscosity, color, and absorbance (232 and 268 nm) of 238 samples of the frying oil collected during 26 days of production were analyzed. For all of the analyses, the results of the oil were below the limits recommended for oil disposal, indicating that ...

  1. Optimasi Dosis Pupuk Dolomit pada Tanaman Kelapa Sawit (Elaeis Guineensis Jacq. Belum Menghasilkan Umur Satu Tahun

    - Sudradjat

    2015-09-01

    Full Text Available Kelapa sawit merupakan komoditi perkebunan utama karena sebagai sumber devisa negara dan menyediakan lapangan kerja. Indonesia adalah negara produsen terbesar kelapa sawit di dunia dengan luas areal mencapai 10.1 juta hektar. Peningkatan produktivitas dicapai dengan intensifikasi antara lain dengan melakukan rasionalisasi pemupkan. Penelitian ini bertujuan untuk menentukan dosis optimum pupuk dolomit pada tanaman kelapa sawit belum menghasilkan umur satu tahun. Penelitian ini dilaksanakan di Kebun Pendidikan dan Penelitian Kelapa Sawit IPB-Cargill, Jonggol, Bogor dari bulan Maret 2013 sampai Maret 2014. Rancangan yang digunakan adalah Faktorial Tunggal,  dosis pupuk dolomit,  yang disusun dalam lingkungan Acak Kelompok dengan tiga ulangan. Dosis pupuk dolomit yang diuji  terdiri atas 0, 200, 400, dan 600 g tanaman-1 tahun-1. Hasil penelitian menunjukkan bahwa pupuk dolomit meningkatkan secara nyata terhadap peubah tinggi tanaman, diameter batang, jumlah daun, dan kandungan khlorofil daun, namun tidak berpengaruh terhadap kandungan Mg dalam jaringan daun. Berdasarkan tanggap peubah tinggi tanaman, dosis optimum pupuk dolomit untuk tanaman kelapa sawit pada umur satu tahun adalah 306.4 g dolomit tanaman-1tahun -1. Kata kunci: dolomit, dosis optimum, kelapa sawit, respons fisiologi, respons morfologi.

  2. In Vitro Callus Induction and Embryogenesis of Oil Palm (Elaeis guineensis Jacq. from Leaf Explants

    DWI HAPSORO

    2011-06-01

    Full Text Available This research was to study in vitro callus induction and somatic embryogenesis in oil palm from leaf explants. Young leaf segments from mature oil palm were cultured on MS medium supplemented with different concentrations of 2,4-D with or without addition of 2 g/l activated charcoal (AC or 2,4-D and picloram. Embryogenesis induction was done using MS medium containing 2,4-D 450 M and benziladenine 4.4 M with 3g/l activated charcoal. The treatment of 2,4-D 15 M resulted in the highest percentage of callus induction. The treatment of 2,4-D and AC showed that 2,4-D 450 M and AC led to higher percentage of callus induction than that of 2,4-D 400 M and 2 g/l AC. Embryogenesis occured in 27 out of 250 clumps of primary callus was occurred after 2-3 times subcultures. Somatic embryo development occurred when the embryogenic callus was transferred on the same basal medium supplemented with casein hydrolysate with 1 M BA or growth regulator free basal medium with 2 g/l activated charcoal.

  3. Aktivitas Enzim Ligninolitik Jamur Dari Tandan Kosong Kelapa Sawit (Elaeis guineensis Jacq.)

    Sitompul, Sepwin Nosten

    2015-01-01

    Lignin adalah polimer alami dan merupakan komponen yang sangat penting penyusun dinding sel tumbuhan. Studi degradasi lignin di lingkungan mendapat perhatian yang cukup besar terutama karena strukturnya yang lebih komplek dan sukarnya molekul tersebut terdegradasi bila dibandingkan dengan degradasi penyusun dinding sel tumbuhan yang lainnya. Kajian untuk mengetahui aktivitas enzim ligninoitik (Lignin Peroksidase, Manganase Peroksidasi dan Lakase) pada jamur yang diisolasi dari tandan kosong k...

  4. Above Ground Biomass (AGB) pada tegakan Sawit (Elaeis guineensis Jacq.) Di Kabupaten Langkat

    Purba, Kepler Dopler

    2015-01-01

    Pemanasan global disebabkan meningkatnya konsentrasi karbon di atmosfir bumi yang melampau konsentrasi alamiahnya. Salah satu upaya pengurangan gas rumah kaca adalah adanya pohon atau tanaman penyerap karbon. Informasi tentang kandungan karbon suatu vegetasi atau tegakan hutan dapat diperoleh dengan menduga biomasa vegetasi tersebut. Teknologi penginderaan jauh serta Sistem Informasi Geografis dirasa cukup memadai dalam memberikan informasi yang dibutuhkan secara cepat dan lengkap dengan tin...

  5. Effect of applied currents to growth in oil palm (Elaeis guineensis Jacq. tissue cultures

    Panote Thavarungkul

    2002-04-01

    Full Text Available External currents of ± 2 μA were applied to the calluses of oil palm in three different culture medium recipes containing different growth substances and the effects of the current were investigated. The three media were medium for callus growth, embryogenetic medium, and organogenetic medium. The stimulation of callus growth was found for both directions of current in one unit of experiments where the embryogenetic culture medium contained NAA. In other cases the use of current seemed to have no significant stimulation effect. The mechanism by which the current may cause the alignment of the cells to promote polar transport of NAA which then increase growth and the relationship of these findings to earlier reports of the stimulation of growth in tobacco callus cultures is discussed.

  6. Protein Profiling and Histone Deacetylation Activities in Somaclonal Variants of Oil Palm (Elaeis guineensis Jacq.

    Jamilah Syafawati Yaacob

    2013-01-01

    Full Text Available Mantled fruits as a result of somaclonal variation are often observed from the oil palm plantlets regenerated via tissue culture. The mantling of fruits with finger-like and thick outer coating phenotypes significantly reduces the seed size and oil content, posing a threat to oil palm planters, and may jeopardize the economic growth of countries that depend particularly on oil palm plantation. The molecular aspects of the occurrence of somaclonal variations are yet to be known, possibly due to gene repression such as DNA methylation, histone methylation and histone deacetylation. Histone deacetylases (HDACs, involved in eukaryotic gene regulation by catalyzing the acetyl groups are removal from lysine residues on histone, hence transcriptionally repress gene expression. This paper described the total protein polymorphism profiles of somaclonal variants of oil palm and the effects of histone deacetylation on this phenomenon. Parallel to the different phenotypes, the protein polymorphism profiles of the mantled samples (leaves, fruits, and florets and the phenotypically normal samples were proven to be different. Higher HDAC activity was found in mantled leaf samples than in the phenotypically normal leaf samples, leading to a preliminary conclusion that histone deacetylation suppressed gene expression and contributed to the development of somaclonal variants.

  7. Multivariate genomic model improves analysis of oil palm (Elaeis guineensis Jacq.) progeny tests

    Marchal, Alexandre; Legarra Albizu, Andres; Tisne, Sebastien; Carasco-Lacombe, Catherine; Manez, Aurore; Suryana, Edyana; Omoré, Alphonse; Nouy, Bruno; Durand-Gasselin, Tristan; Sanchez, Leopoldo; Bouvet, Jean-Marc; Cros, David

    2016-01-01

    Genomic selection is promising for plant breeding, particularly for perennial crops. Multivariate analysis, which considers several traits jointly, takes advantage of the genetic correlations to increase accuracy. The aim of this study was to empirically evaluate the potential of a univariate and multivariate genomic mixed model (G-BLUP) compared to the traditional univariate pedigree-based BLUP (T-BLUP) when analyzing progeny tests of oil palm, the world’s major oil crop. The dataset compris...

  8. Alterations of palm oil (Elaeis guineensis in the continuous industrial par frying of breaded chicken snacks

    Débora Rezende Ferreira

    2014-03-01

    Full Text Available The physical and chemical alterations in palm oil during continuous industrial par frying of breaded chicken snacks were evaluated using a pseudo first-order kinetic model. The acidity index, refractive index, concentration of polar compounds, viscosity, color, and absorbance (232 and 268 nm of 238 samples of the frying oil collected during 26 days of production were analyzed. For all of the analyses, the results of the oil were below the limits recommended for oil disposal, indicating that the processing conditions were safe and that under these experimental conditions the oil remained suitable for frying. The linear regressions were significant for refractive index, content of polar compounds, and lightness (L*. The content of polar compounds was determined using a cooking oil tester, and it had the best fit to the proposed model and can be used as an effective index for monitoring palm oil during the continuous par frying of breaded chicken snacks. The high turnover rate of the oil was important for maintaining the oil in good running conditions.

  9. Natural (13) C distribution in oil palm (Elaeis guineensis Jacq.) and consequences for allocation pattern.

    Lamade, Emmanuelle; Tcherkez, Guillaume; Darlan, Nuzul Hijri; Rodrigues, Rosario Lobato; Fresneau, Chantal; Mauve, Caroline; Lamothe-Sibold, Marlène; Sketriené, Diana; Ghashghaie, Jaleh

    2016-01-01

    Oil palm has now become one of the most important crops, palm oil representing nearly 25% of global plant oil consumption. Many studies have thus addressed oil palm ecophysiology and photosynthesis-based models of carbon allocation have been used. However, there is a lack of experimental data on carbon fixation and redistribution within palm trees, and important C-sinks have not been fully characterized yet. Here, we carried out extensive measurement of natural (13) C-abundance (δ(13) C) in oil palm tissues, including fruits at different maturation stages. We find a (13) C-enrichment in heterotrophic organs compared to mature leaves, with roots being the most (13) C-enriched. The δ(13) C in fruits decreased during maturation, reflecting the accumulation in (13) C-depleted lipids. We further used observed δ(13) C values to compute plausible carbon fluxes using a steady-state model of (13) C-distribution including metabolic isotope effects ((12) v/(13) v). The results suggest that fruits represent a major respiratory loss (≈39% of total tree respiration) and that sink organs such as fruits are fed by sucrose from leaves. That is, glucose appears to be a quantitatively important compound in palm tissues, but computations indicate that it is involved in dynamic starch metabolism rather that C-exchange between organs. PMID:26228944

  10. Pembuatan Asam Oksalat Dari Pelepah Kelapa Sawit (Elaeis guineensis) Menggunakan Metode Peleburan Alkali

    Hasibuan, M. Hidayat

    2016-01-01

    Palm frond is one of the solid waste from oil palm plantations which contains lignocellulose namely cellulose, hemicellulose, and lignin. Cellulose from waste palm fronds can be further processed into useful products and economic value, one of which is used as raw material for making oxalic acid. The purpose of this research is assess the effect of temperature and reaction time in the hydrolysis of palm fronds with alkali fusion method of producing oxalic acid. The research consisted of two s...

  11. Optimasi Dosis Pupuk Dolomit pada Tanaman Kelapa Sawit (Elaeis Guineensis Jacq.) Belum Menghasilkan Umur Satu Tahun

    - Sudradjat; - Fitriya

    2015-01-01

    Kelapa sawit merupakan komoditi perkebunan utama karena sebagai sumber devisa negara dan menyediakan lapangan kerja. Indonesia adalah negara produsen terbesar kelapa sawit di dunia dengan luas areal mencapai 10.1 juta hektar. Peningkatan produktivitas dicapai dengan intensifikasi antara lain dengan melakukan rasionalisasi pemupkan. Penelitian ini bertujuan untuk menentukan dosis optimum pupuk dolomit pada tanaman kelapa sawit belum menghasilkan umur satu tahun. Penelitian ini dilaksanakan di ...

  12. Microbial Inoculation Improves Growth of Oil Palm Plants (Elaeis guineensis Jacq.)

    Om, Azlin Che; Ghazali, Amir Hamzah Ahmad; Keng, Chan Lai; Ishak, Zamzuri

    2009-01-01

    Introduction of diazotrophic rhizobacteria to oil palm tissues during the in vitro micropropagation process establishes an early associative interaction between the plant cells and bacteria. In the association, the diazotrophs provide the host plants with phytohormones and fixed nitrogen. This study was conducted to observe growth of bacterised tissue cultured oil palm plants under ex vitro conditions after 280 days of growth. Root dry weight, shoot dry weight, root volume, bacterial colonisa...

  13. Spatial undergrowth species composition in oil palm (Elaeis guineensis Jacq.) in West Sumatra

    Germer, Jörn Uwe

    2003-01-01

    The area planted to oil palm expanded during the last decades substantially, making it become the world's second most important oil crop. Despite its economic significance the oil palm remains remarkably unknown. Little attention is paid also to the oil palm undergrowth, though important in stabilizing the agro-ecosystem in plantations. Comprehensive knowledge of undergrowth species adapted to specific ecological niches in oil palm plantations is essential to investigate their function in ...

  14. Spectral reflectance of oil-palm (Elaeis guineensis Jacq.) bunches as indicator for optimal harvesting

    Wunsri, S.; Chittrakarn, T.; Bhongsuwan, T.; Kongsaeng, S.

    2005-01-01

    To determine the appropriate harvesting time of Tenera oil palm bunches, the spectral reflectance of ripening bunches was investigated from images taken by a digital Canon PowerShot A20 camera. Every second day images were taken from selected bunches at an oil-palm estate during the growth of the bunchesfrom immaturity to the over-ripe phase, to trace the changes in color which correlate with the process of ripening. The images were analyzed measuring the changes in the three basic colors red...

  15. Hydrolysis of Palm Kernel Cake (Elaeis guineensis Jacq) by Fungi Trichoderma reesei that Degrades Mannan Polysaccharides

    Achmad Jaelani; Wiranda Gentini Piliang; Suryahadi; Iman Rahayu

    2008-01-01

    The research was conducted to study the growth characterization of Trichoderma reesei, the degradability of mannan polysaccharides from Palm Kernel Cake (PKC) with different concentrations of fungi and thickness of the PKC medium, as well as the improvement of the nutritive value of palm kernel cake. Experiment 1 was to study the growth characterization of Trichoderma reesei, and Experiment 2 was conducted using concentrations of fungi (104, 105, and 106 CFU per cc) and thickness of the PKC m...

  16. Correlation, path coefficient analysis and heritability for agronomic characters of oil palm (Elaeis guineensis Jacq.

    Chaumongkol, Y.

    2001-11-01

    Full Text Available A study of correlation, path coefficient analysis and heritablity for some agronomic characters of oil palm was investigated during February 1998 to January 2002. The oil palm population used in this experiment was derived from F1 tenera hybrids which were collected from various oil palm plantations in Southern Thailand. One good performance bunch (i.e., big bunch, thin shell was selected from each plantation and four to six seeds per selected bunch were used for cultivation. One thousand thirty eight plants were grown at Klong Hoi Khong Research Station, Faculty of Natural Resources, Prince of Songkla University, Songkhla, in 1989. Forty five palms consisted of Dura, Tenera and Pisifera types with 18, 18 and 9 plants respectively, were selected by randomization and tagged for investigation. The oil palm bunch yield and yield component characters were observed from individual palm for 4 years (February 1998 to January 2002. The bunch composition characters were analysed from a single bunch of each palm, sampled between June to October 1999. The results showed that in F2 plants of oil palm, the correlation and the path coefficient between characters relating to oil yield and %oil/bunch varied according to oil palm types (Dura, Tenera and Pisifera. In Dura and Tenera palms, the characters which gave highly positive correlation with a large direct and indirect positive effects on oil yield and %oil/bunch were total bunch weight, %oil/bunch, %fruit/bunch and %oil/fruit. In case of Pisifera palms, the characters which gave highly positive correlation with a large direct and indirect positive effects on oil yield and %oil/bunch were total bunch weight, number of bunches, single bunch weight, %oil/bunch and %fruit/bunch. However, from all investigated characters in F2 plants, only %mesocarp/fruit, %oil/fruit and %fruit/bunch showed the high values of broad sense heritabilities.

  17. Assaying lipase activity from oil palm fruit (Elaeis guineensis Jacq.) mesocarp.

    Ngando Ebongue, G F; Dhouib, R; Carrière, F; Amvam Zollo, P-H; Arondel, V

    2006-10-01

    The mesocarp of mature oil palm fruit undergoes intensive triglycerides hydrolysis upon abscission and bruising. This generates such a high amount of free fatty acids that the oil might become unfit for human consumption without appropriate refining. The lipase (EC 3.1.1.3) involved in the breakdown of the oil is not stable after homogenization of the tissue in aqueous buffers. In this study, we have devised a solvent-based procedure that allowed us to obtain fractions with stable lipase activity. Using these fractions, we have determined the optimal conditions for assaying mesocarp lipase activity. The activity was highest at a temperature of 35 degrees C and a pH of 9. The lipase was found to be strictly calcium dependent. The specific activity of the lipase measured in optimal conditions was found to be 33 mumol fatty acids released min(-1) mg(-1) protein using olive oil as substrate. The mesocarp contains about 190 U of lipase g(-1) fresh weight. This activity was found to be inhibited by the lipase inhibitor tetrahydrolipstatin (THL), suggesting that the lipase is a serine hydrolase. PMID:17064925

  18. Correlation, path coefficient analysis and heritability for agronomic characters of oil palm (Elaeis guineensis Jacq.)

    Chaumongkol, Y.; Nilnond, C.; Tongkum, P.; Juntaraniyom, T.; Songsri, N.; Eksomtramage, T.

    2001-01-01

    A study of correlation, path coefficient analysis and heritablity for some agronomic characters of oil palm was investigated during February 1998 to January 2002. The oil palm population used in this experiment was derived from F1 tenera hybrids which were collected from various oil palm plantations in Southern Thailand. One good performance bunch (i.e., big bunch, thin shell) was selected from each plantation and four to six seeds per selected bunch were used for cultivation. One thousand th...

  19. Association of lignifying enzymes in shell synthesis of oil palm fruit (Elaeis guineensis--dura variety).

    Bhasker, S; Mohankumar, C

    2001-02-01

    Scanning electron microscopic (SEM) observation demonstrates the differentiation of mesocarp and endocarp tissues and their lignified nature in dura fruits at 8 weeks after pollination (WAP). During shell formation, the endocarp cells become lignified to a hard shell while the mesocarp tissue remains cellular and fibrous. A transition zone made up of fibrous units was also visible beneath the shell. The soluble phenols of mesocarp and endocarp tissues at their developmental stage was analyzed using Reverse phase high performance liquid chromatography (RP-HPLC). The appearance of ferulic acid at 4 WAP and its absence at 8 WAP indicates the role of ferulic acid in lignin synthesis. The HPLC data was supported by the lignin concentration. To ascertain the biochemical relationship of lignin pathway enzymes, phenylalanine ammonia lyase (PAL), cinnamyl alcohol-NADPH-dehydrogenase (CAD) and peroxidase (POD) with shell synthesis, the activities of these enzymes and lignin content were assessed during development of the shell between 4 and 8 WAP. The three enzymes, PAL, CAD and POD expressed high level of activity in the mesocarp and endocarp at 4 WAP. At 8 WAP a sharp decline in activity was observed in the endocarp whereas the mesocarp showed a moderate reduction. This variation is an indication of the role of these enzymes in shell formation. PMID:11480213

  20. Análisis de las últimas investigaciones sobre pudrición del cogollo en palma de aceite (Elaeis guineensis jacq) / Analysis of the last researchs concerning bud rotting in oil palm (Elaeis guineensis jacq)

    Santos Rubio, Álvaro Eduardo

    2010-01-01

    La pudrición de cogollo es una enfermedad compleja que afecta a la mayoría de los materiales de palma de aceite y que puede ser muy severa dependiendo de las características de la zona en que se encuentre. Es responsable de grandes pérdidas de algunas plantaciones. Por muchas décadas el agente causal de la enfermedad fue relacionado con microorganismos como hongos, virus, bacterias, factores climáticos, desordenes fisiológicos y nutricionales, problemas de suelos e insectos. La enfermedad por...

  1. Pengaruh Tingkat Media Tanam dan Pupuk Daun Grofas Terhadap Pertumbuhan Bibit Kelapa Sawit (Elaeis guineensis Jacq) di Main Nursery

    Wijaya, Hardy

    2012-01-01

    Tujuan penelitian ini untuk mengetahui pengaruh tingkat media tanam dan pupuk daun Grofas terhadap pertumbuhan bibit kelapa sawit di main nursery. Penelitian dimulai dari bulan November 1999 sampai dengan Februari 2000. Rancangan yang digunakan adalah Rancangan Aeak Kelompok faktorial dengan 2 faktor dengan 3 ulangan.

  2. Pengaruh Pemberian Pupuk Microcytan dan ZPT Atonik Terhadap Pertumbuhan Bibit Kelapa Sawit (Elaeis guineensis Jacq) di Pembibitan, Utama

    Siregar, Muhammad Hidayat

    2012-01-01

    Penelitian ini di laksanakan di kebun percobaan Fakultas Pertanian UISU Kecamatan Medan Johor dengan ketinggian ternpat 25 meter di atas permukaan laut. Penelitian ini berlangsung dari bulan Maret 2001 sampai dengan bulan Juni 2001.

  3. Pengaruh Pupuk Nitrophoska (15: 15: 15) Dan Pupuk cair TNF Terhadap Pertumbuhan Vegetatif Bibit Kelapa Sawit (Elaeis guineensis Jaeq )

    Tarigan, Hendrix

    2012-01-01

    Tujuan dari penelitian ini adalah untuk mengetahui Pengaruh Pernberian Pupuk Ni trophoska dan Pupuk Cair TNF terhadap Pertumbuhan Bibit Kelapa Sawit di Pembibitan Utara. Penelitian ini dilaksanakan di Jalan Bunga Ncole keeamatan Medan Tuntungan,Medan. Berlangsung dari bulan Mei hingga bulan Agustus 200O. Rancangan yang digunakan adalah Rancangan Acak Kelonpok (RAK) Faktorial, terdiri dari dua faktor perlakuan dan tiga ulangan.

  4. Expression profiles of putative defence-related proteins in oil palm (Elaeis guineensis) colonized by Ganoderma boninense.

    Tan, Yung-Chie; Yeoh, Keat-Ai; Wong, Mui-Yun; Ho, Chai-Ling

    2013-11-01

    Basal stem rot (BSR) is a major disease of oil palm caused by a pathogenic fungus, Ganoderma boninense. However, the interaction between the host plant and its pathogen is not well characterized. To better understand the response of oil palm to G. boninense, transcript profiles of eleven putative defence-related genes from oil palm were measured by quantitative reverse-transcription (qRT)-PCR in the roots of oil palms treated with G. boninense from 3 to 12 weeks post infection (wpi). These transcripts encode putative Bowman-Birk serine protease inhibitors (EgBBI1 and 2), defensin (EgDFS), dehydrin (EgDHN), early methionine-labeled polypeptides (EgEMLP1 and 2), glycine-rich RNA binding protein (EgGRRBP), isoflavone reductase (EgIFR), metallothionein-like protein (EgMT), pathogenesis-related-1 protein (EgPRP), and type 2 ribosome-inactivating protein (EgT2RIP). The transcript abundance of EgBBI2 increased in G. boninense-treated roots at 3 and 6wpi compared to those of controls; while the transcript abundance of EgBBI1, EgDFS, EgEMLP1, EgMT, and EgT2RIP increased in G. boninense-treated roots at 6 or 12wpi. Meanwhile, the gene expression of EgDHN was up-regulated at all three time points in G. boninense-treated roots. The expression profiles of the eleven transcripts were also studied in leaf samples upon inoculation of G. boninense and Trichoderma harzianum to identify potential biomarkers for early detection of BSR. Two candidate genes (EgEMLP1 and EgMT) that have different profiles in G. boninense-treated leaves compared to those infected by T. harzianum may have the potential to be developed as biomarkers for early detection of G. boninense infection. PMID:23769496

  5. Kajian Musuh Alami Serangga Penyerbuk Kelapa Sawit Elaeidobius kamerunicus Faust. (Coleoptera : curculionidae) Pada Tanaman Kelapa Sawit (Elaeis guineensis Jacq.)

    Reh Ucina Sitepu

    2009-01-01

    Penelitian ini bertujuan untuk mengetahui musuh alami serangga penyerbuk kelapa sawit E. kamerunicus Faust. Penelitian dilaksanakan di Laboratorium Hama Fakultas Pertanian Universitas Sumatera Utara Medan. Penelitian ini dilaksanakan pada bulan Februari sampai dengan Mei 2007. Dari hasil penelitian ditemukan parasitoid E. kamerunicus adalah ordo Diptera famili Tachinidae pada larva sebanyak 2 ekor (0,5%) dan pupa 1 ekor (0,2%). Disamping itu, munculnya M. anisopliae sebagai entomopatogen ...

  6. Effect of source/sink ratios on yield components, growth dynamics and structural characteristics of oil palm (Elaeis guineensis) bunches.

    Pallas, Benoît; Mialet-Serra, Isabelle; Rouan, Lauriane; Clément-Vidal, Anne; Caliman, Jean-Pierre; Dingkuhn, Michael

    2013-04-01

    Source/sink ratios are known to be one of the main determinants of oil palm growth and development. A long-term experiment (9 years) was conducted in Indonesia on mature oil palms subjected to continuous bunch ablation and partial defoliation treatments to artificially modify source/sink ratios. During the experiment, all harvested bunches were dissected and phenological measurements were carried out to analyse the effect of source/sink ratios on yield components explaining variations in bunch number, the number of fruits per bunch and oil dry weight per fruit. An integrative variable (supply/demand ratio) describing the ratio between the assimilate supply from sources and the growing organ demand for carbohydrate was computed for each plant on a daily basis from observations of the number of developing organs and their sink strength, and of climate variables. Defoliation and bunch ablation affected the bunch number and the fruit number per bunch. Variations in bunch number per month were mainly due to variations in the fraction of aborted inflorescence and in the ratio between female and male inflorescences. Under fluctuating trophic conditions, variations in fruit number per bunch resulted both from changes in fruit-set and in the number of branches (rachillae) per inflorescence. For defoliated plants, the decrease in the number of developing reproductive sinks appeared to be sufficient to maintain fruit weight and oil concentration at the control level, without any major decrease in the concentration of non-structural carbohydrate reserves. Computation of the supply/demand ratio revealed that each yield component had a specific phase of sensitivity to supply/demand ratios during inflorescence development. Establishing quantitative relationships between supply/demand ratios, competition and yield components is the first step towards a functional model for oil palm. PMID:23532136

  7. Pengaruh Pemberian N-Fix dan Pupuk Daun TNF Terhadap Pertumbuhan Vegetatif Bibit Kelapa Sawit (Elaeis Guineensis Jacq.)

    Siregar, M.Fadli

    2012-01-01

    Penelitian ini dilaksanakan di kebun percobaan Fakultas Pertanian Universitas Islam Sumatera Utara kecamatan Medan Johor, kotamadya Medan dengan ketinggian tempat lebih kurang 25 meter di atas permukaan laut, berlangsung dari bulan April 2000 sampai bulan Juli 2000. Parameter yang diamati adalah pertambahan tinggi bibit (an), pertambahan jumlah daun (helai), pertambahan diameter batang (mm) , pertambahan total luas pertambahan jumlah klorofil (/6 mm2 ).

  8. In vivo analysis of palm wine (Elaeis guineensis) volatile organic compounds (VOCs) by proton transfer reaction-mass spectrometry

    Lasekan, Ola; Otto, Sabine

    2009-04-01

    The in vivo volatile organic compounds (VOCs) release patterns in palm wine was carried out using the PTR-MS. In order to analyze the complex mixtures of VOCs in palm wine, the fragmentation patterns of 14 known aroma compounds of palm wine were also investigated. Results revealed masses m/z (43, 47, 61, 65, 75, 89 and 93) as the predominant ones measured in-breathe exhaled from the nose, during consumption of palm wine. Further studies of aroma's fragmentation patterns, showed that the m/z 43 is characteristic of fragment of various compounds, while m/z 47 is ethanol, m/z 61(acetic acid), m/z 65 (protonated ethanol cluster ions), m/z 75 (methyl acetate), m/z 89 (acetoin) and m/z 93 (2-phenylethanol) respectively. The dynamic release parameters (Imax and tmax) of the 7 masses revealed significant (P = 0.05) differences, between maximum intensity (Imax) and no significant (P = 0.05) differences between tmax among VOCs respectively.

  9. Metabolism of cytokinins by tissue culture lines of oil palm (Elaeis guineensis Jacq.) producing normal and abnormal flowering palms

    The metabolism of cytokinins in tissue cultures of two oil palm clones previously known to regenerate palms ultimately manifesting normal and abnormal flowering was studied using radiolabeled benzyladenine and isopentenyladenosine, with particular regard to the kinetics of formation of the cytokinin 9-glucoside. Labeled products were separated by high performance liquid chromatography and identified by comparison of retention times with authentic cytokinin standards run immediately before or after the experimental sample. Using benzyladenine, which is insensitive to cytokinin oxidase, ribotide appeared rapidly and then declined. 6-Benzylaminopurine (BA) 9-glucoside quickly became the major soluble product with some formation of riboside. No other ethanol-soluble products were found. Over an incubation period of 24 h up to 30% of label appeared in the ethanol-insoluble fraction. The uptake of label was consistently faster in the normal than the abnormal clone. Dose-rate and time course experiments produced an in vivo asymptotic dose-response curve for the accumulation of BA 9-glucoside analogous to a Michaelis-Menten first-order reaction with a 'V(max)' of 3.5 nmol.g-1.h-1 (on a fresh weight basis) and a 'K(m)' of 0.12 mM. There were no differences between clones in the rate of synthesis. Using isopentenyladenosine, which is susceptible to cytokinin oxidase and cannot be glucosylated without prior deribosylation, a complex pattern of metabolism was seen, with much slower production of 9-glucoside. A number of transient unidentified compounds were seen, together with adenosine and adenine. Comparison of normal and abnormal flowering clones showed striking differences in the kinetics of production of a compound thought to be [9R]Z and in a transient compound eluting at 22 min which accounted for 42% of the radioactivity after a 7-h incubation in the abnormal line. By 17 h there was no difference between normal and abnormal lines in the radioactivity in this compound

  10. Pengaruh Elevasi Lahan dan Posisi Pelepah Terhadap Anatomi dan Sifat Fisik pada Fenomena Pelepah Sengkleh Kelapa Sawit (Elaeis guineensis Jacq.)

    Janiyani, Lily

    2015-01-01

    Frond is a section in oil palm tree besides of flower and fruit. The cultivation oil palm were in many elevation. Frond fracture in palm oil was can cause the stop growth in plants. Frond fracture was can attacked the plants produced so as to decrease the value or the production of oil palm. This study was aim to provided information about the anatomical, physical properties as density of vascular bundle, moisture content, frond the weight and length of palm. For this purpose was a total of 1...

  11. Efecto de la lipofilización sobre las propiedades funcionales de la harina de palmiste (Elaeis guineensis

    Barrera-Arellano, Daniel

    2005-03-01

    Full Text Available Functional properties of proteins presented in palm kernel flour were studied, started with almonds with and without testa. Removal of testa with HCl permitted a decrease of the polyphenol concentration, besides conferring the flour a light cream color. Lipophilization of the proteins of palm kernel was studied in order to evaluate the changes on functional properties and compare them to the properties of non-modified flour. Properties measured were solubility, water retention capacity (WRC and oil retention capacity (ORC, emulsion capacity (EC, emulsion activity (EA, emulsion stability (ES, foam expansion (FE and foam stability (FS. Lipophilization of the cake without testa was tested in two protein:palmitoyl chloride ratios: 1:0.5 and 1:1, w/v. Solubility and EC decreased, however, the other properties either kept constant (WRC or increased markedly. Lipophilization with a lower concentration of fatty acids (1:0.5 protein:palmitoyl chloride ratio showed higher values for functional properties.Se estudiaron las propiedades funcionales de las proteínas presentes en la harina de palmiste, obtenida a partir de almendras con y sin testa. La remoción de la testa con HCl 4N permitió disminuir la concentración de polifenoles, lo que significó obtener una harina de mejor color (crema claro y textura. Se estudió la lipofilización de las proteínas de la harina de palmiste para evaluar los cambios en las propiedades funcionales y compararlos con las propiedades de las harinas no modificadas. Las propiedades medidas fueron: solubilidad, capacidad de retención de agua (CRA y capacidad de retención de aceite (CRAC, capacidad emulsionante (CE, actividad emulsionante (AE, estabilidad de la emulsión (EE, expansión de la espuma (EEsp y estabilidad de la espuma (EsEsp. La lipofilización de la harina sin testa se realizó en dos proporciones de proteína/cloruro de ácido palmítico, 1:0,5 y 1:1, p/v. La solubilidad y la CE disminuyeron; sin embargo, las otras propiedades se mantuvieron constantes (CRA o bien aumentaron notablemente. La lipofilización con baja concentración de ácidos grasos (relación proteína/cloruro de 1:0,5 generó productos con mayores valores en sus propiedades funcionales.

  12. Analysis and functional annotation of expressed sequence tags (ESTs from multiple tissues of oil palm (Elaeis guineensis Jacq.

    Lee Weng-Wah

    2007-10-01

    Full Text Available Abstract Background Oil palm is the second largest source of edible oil which contributes to approximately 20% of the world's production of oils and fats. In order to understand the molecular biology involved in in vitro propagation, flowering, efficient utilization of nitrogen sources and root diseases, we have initiated an expressed sequence tag (EST analysis on oil palm. Results In this study, six cDNA libraries from oil palm zygotic embryos, suspension cells, shoot apical meristems, young flowers, mature flowers and roots, were constructed. We have generated a total of 14537 expressed sequence tags (ESTs from these libraries, from which 6464 tentative unique contigs (TUCs and 2129 singletons were obtained. Approximately 6008 of these tentative unique genes (TUGs have significant matches to the non-redundant protein database, from which 2361 were assigned to one or more Gene Ontology categories. Predominant transcripts and differentially expressed genes were identified in multiple oil palm tissues. Homologues of genes involved in many aspects of flower development were also identified among the EST collection, such as CONSTANS-like, AGAMOUS-like (AGL2, AGL20, LFY-like, SQUAMOSA, SQUAMOSA binding protein (SBP etc. Majority of them are the first representatives in oil palm, providing opportunities to explore the cause of epigenetic homeotic flowering abnormality in oil palm, given the importance of flowering in fruit production. The transcript levels of two flowering-related genes, EgSBP and EgSEP were analysed in the flower tissues of various developmental stages. Gene homologues for enzymes involved in oil biosynthesis, utilization of nitrogen sources, and scavenging of oxygen radicals, were also uncovered among the oil palm ESTs. Conclusion The EST sequences generated will allow comparative genomic studies between oil palm and other monocotyledonous and dicotyledonous plants, development of gene-targeted markers for the reference genetic map, design and fabrication of DNA array for future studies of oil palm. The outcomes of such studies will contribute to oil palm improvements through the establishment of breeding program using marker-assisted selection, development of diagnostic assays using gene targeted markers, and discovery of candidate genes related to important agronomic traits of oil palm.

  13. Development of SNP markers and their application for genetic diversity analysis in the oil palm (Elaeis guineensis).

    Ong, P W; Maizura, I; Abdullah, N A P; Rafii, M Y; Ooi, L C L; Low, E T L; Singh, R

    2015-01-01

    The genetic evaluation of oil palm germplasm collections is required for insight into the variability among populations. The information obtained is also useful for incorporating new genetic materials into current breeding programs. Single nucleotide polymorphisms (SNPs) have been widely used in many plant genetic studies due to the availability of large numbers of genomic sequences and expressed sequence tags. The present study examined 219 oil palms collected from two natural Angolan populations, a few hundred kilometers apart. A total of 62 SNPs were designed from oil palm genomic sequences and converted to cleaved amplified polymorphic sequence (CAPS). Of these, nine were found to be informative across the two populations. The nine informative SNPs revealed mean major allele frequency of 0.693. The average expected and observed heterozygosities were 0.398 and 0.400, respectively. The mean polymorphism information content was 0.315 (ranging between 0.223 and 0.375). None of the loci deviated from Hardy-Weinberg equilibrium and no rare alleles were detected. In cluster analysis using unweighted pair group method with arithmetic, the 219 oil palms fell into two clusters. This was further supported by the population structure analysis result (K = 2), suggesting that the samples were divided into two main genetic groups. However, the two groups did not coincide with the geographic populations. Analysis of molecular variance indicated that within-population variation contributed 93% of the total genetic variation. This study showed that SNP-based CAPS markers are useful for studying the genetic diversity of oil palm and have potential application for marker-trait association studies. PMID:26505369

  14. Optimization of aqueous enzymatic oil extraction from kernel of oil palm (elaeis guineensis) using three phase partitioning and microwave

    The use of microwave irradiation as a pretreatment before aqueous enzymatic oil extraction from oil palm kernel was found to be useful. The microwave irradiation for 10 min -assisted extraction was found to be a simpler and more effective alternative to the solvent extraction methods for the productions of palm kernel oil. Further enhancement was achieved when the microwave irradiated slurries were treated with a commercial enzyme preparation of proteases, followed by three phase partitioning. This resulted in 93% (w/w) oil yields form the palm kernel. The efficiency of the present technique is comparable to solvent extraction with an added advantage of being less time consuming and using t-butanol which is a safer solvent as compared to n-hexane used in conventional oil extraction process. The technique also tries to reduce the amount of enzyme used and hence reduces the overall cost. (author)

  15. Phenotypic Diversity Analysis of Oil Palm (Elaeis guineensis Jacq.) Germplasm Cameroon Origin Based on Vegetative and Physiologic Characteristic

    Lubis, Mahmud Irfan; Putri, Lollie Agustina P.; Purba, Abdul Razak

    2016-01-01

    Forty seven accessions as a part of of oil palm germplasm Cameroon Origin is planted at Adolina, an estate of PT Perkebunan Nusantara IV were evaluated for the variability based on their early vegetative and physiology characteristics. Mean data from these accessions were analysed by Multivarite Analysis consist of Principal Component Analysis (PCA), revealing their variability and distribution. Results demonstrated that principal component analysis obtained two main components to contribute ...

  16. Over expression and application of the -carboxyltransferase (EgaccD) gene in oil palm (Elaeis guineensis Jacq)

    Alisa Nakkaew; Amornrat Phongdara; Theera Eksomtramage; Wilaiwan Chotigeat

    2014-01-01

    Oil palm is an economic crop with its oil currently in high demand. In this work, the numbers of EgaccD copies, one of the key genes involved in fatty acid synthesis and oil production, were compared from two populations of oil palm, one a low and the other a high oil content productivity cultivar. Real time PCR was employed and the copy number of the EgaccD from the high oil yielding cultivar was much higher than from the low oil yielding cultivar. The EgaccD was then over expressed...

  17. Production of polyhydroxybutyrate in oil palm (Elaeis guineensis Jacq.) mediated by microprojectile bombardment of PHB biosynthesis genes into embryogenic calli

    Parveez, Ghulam Kadir Ahmad; Bahariah, Bohari; Ayub, Nor Hanin; Masani, Mat Yunus Abdul; Rasid, Omar Abdul; Tarmizi, Ahmad Hashim; Ishak, Zamzuri

    2015-01-01

    Biodegradable plastics, mainly polyhydroxybutyrate (PHB), which are traditionally produced by bacterial cells, have been produced in the cells of more than 15 plant species. Since the production of biodegradable plastics and the synthesis of oil in plants share the same substrate, acetyl-coenzyme A (acetyl-CoA), producing PHB in oil bearing crops, such as oil palm, will be advantageous. In this study, three bacterial genes, bktB, phaB, and phaC, which are required for the synthesis of PHB and...

  18. Production of polyhydroxybutyrate in oil palm (Elaeis guineensis Jacq. mediated by microprojectile bombardment of PHB biosynthesis genes into embryogenic calli

    Ahmad Parveez eGhulam Kadir

    2015-08-01

    Full Text Available Biodegradable plastics, mainly polyhydroxybutyrate (PHB, which are traditionally produced by bacterial cells, have been produced in the cells of more than 15 plant species. Since the production of biodegradable plastics and the synthesis of oil in plants share the same substrate, acetyl-coenzyme A (acetyl-CoA, producing PHB in oil bearing crops, such as oil palm, will be advantageous. In this study, three bacterial genes, bktB, phaB and phaC, which are required for the synthesis of PHB and selectable marker gene, bar, for herbicide Basta resistant, were transformed into embryogenic calli. A number of transformed embryogenic lines resistant to herbicide Basta were obtained and were later regenerated to produce few hundred plantlets. Molecular analyses, including PCR, Southern blot and real-time PCR have demonstrated stable integration and expression of the transgenes in the oil palm genome. HPLC and Nile Blue A staining analyses confirmed the synthesis of PHB in some of the plantlets.

  19. Over expression and application of the -carboxyltransferase (EgaccD gene in oil palm (Elaeis guineensis Jacq

    Alisa Nakkaew

    2014-02-01

    Full Text Available Oil palm is an economic crop with its oil currently in high demand. In this work, the numbers of EgaccD copies, one of the key genes involved in fatty acid synthesis and oil production, were compared from two populations of oil palm, one a low and the other a high oil content productivity cultivar. Real time PCR was employed and the copy number of the EgaccD from the high oil yielding cultivar was much higher than from the low oil yielding cultivar. The EgaccD was then over expressed in oil palm calli under the control of its promoter to establish if its overexpression could enhance the oil content of transgenic calli. An increase in the lipid accumulation by the modified calli was detected by Sudan black B staining. Based on this finding, genetic manipulation of EgaccD seemed to be one promising method to try to increase the oil content. Also it seemed possible to use the level of this gene as a marker to assist selection of possible high oil yielding cultivars in breeding programs.

  20. Respons Pertumbuhan Vegetatif Tiga Varietas Kelapa Sawit (Elaeis Guineensis Jacq.) Di Pre Nursery Pada Beberapa Komposisi Media Tanam Limbah

    Afrillah, Muhammad

    2015-01-01

    The aim of the research was is to study vegetative growth response of three varieties of palm oil tree at some palm waste as planting medium in pre nursery.The research has been conducted in Kompleks Medan permai on November 2014 to February 2015. The research design used randomized block design with two factor. The first factor is composition palm waste for planting medium (top soil, top soil + fiber 1:1, top soil + empty fruit bunches1:1, top soil + solid decanter 1:1 and top soil + fiber +...

  1. Camera-Vision Based Oil Content Prediction for Oil Palm (Elaeis Guineensis Jacq) Fresh Fruits Bunch at Various Recording Distances

    Dinah Cherie; Sam Herodian; Tineke Mandang; Usman Ahmad

    2015-01-01

    In this study, the correlation between oil palm fresh fruits bunch (FFB) appearance and its oil content (OC) was explored. FFB samples were recorded from various distance (2, 7, and 10 m) with different lighting spectrums and configurations (Ultraviolet: 280-380nm, Visible: 400-700nm, and Infrared: 720-1100nm) and intensities (600watt and 1000watt lamps) to explore the correlations. The recorded FFB images were segmented and its color features were subsequently extracted to be used as input v...

  2. Production of polyhydroxybutyrate in oil palm (Elaeis guineensis Jacq.) mediated by microprojectile bombardment of PHB biosynthesis genes into embryogenic calli.

    Parveez, Ghulam Kadir Ahmad; Bahariah, Bohari; Ayub, Nor Hanin; Masani, Mat Yunus Abdul; Rasid, Omar Abdul; Tarmizi, Ahmad Hashim; Ishak, Zamzuri

    2015-01-01

    Biodegradable plastics, mainly polyhydroxybutyrate (PHB), which are traditionally produced by bacterial cells, have been produced in the cells of more than 15 plant species. Since the production of biodegradable plastics and the synthesis of oil in plants share the same substrate, acetyl-coenzyme A (acetyl-CoA), producing PHB in oil bearing crops, such as oil palm, will be advantageous. In this study, three bacterial genes, bktB, phaB, and phaC, which are required for the synthesis of PHB and selectable marker gene, bar, for herbicide Basta resistant, were transformed into embryogenic calli. A number of transformed embryogenic lines resistant to herbicide Basta were obtained and were later regenerated to produce few hundred plantlets. Molecular analyses, including polymerase chain reaction (PCR), Southern blot, and real-time PCR have demonstrated stable integration and expression of the transgenes in the oil palm genome. HPLC and Nile blue A staining analyses confirmed the synthesis of PHB in some of the plantlets. PMID:26322053

  3. Spatial variability of some soil properties varies in oil palm (Elaeis guineensis Jacq.) plantations of west coastal area of India

    Behera, Sanjib Kumar; Suresh, Kancherla; Rao, Bezawada Narsimha; Mathur, Ravi Kumar; Shukla, Arvind Kumar; Manorama, Kamireddy; Ramachandrudu, Kummari; Harinarayana, Parasa; Prakash, Chandra

    2016-01-01

    Mapping spatial variability of soil properties is the key to efficient soil resource management for sustainable crop yield. Therefore, the present study was conducted to assess the spatial variability of soil properties such as acidity (pH), salinity (electrical conductivity (EC)), organic carbon, available K, available P, exchangeable Ca2+, exchangeable Mg2+, available S and hot water soluble B in surface (0–20 cm) and subsurface (20–40 cm) soil layers of oil palm plantatio...

  4. Determination of the most efficient target tissue and helium pressure for biolistic transformation of oil palm (Elaeis guineensis Jacq.

    Amornrat Phongdara

    2008-03-01

    Full Text Available An efficient genetic transformation system for oil palm using particle bombardment was established. The transformation was performed using the pCAMBIA 1302 DNA which contains the green fluorescent protein (mgfp5 reporter gene and the selectable marker hygromycin phosphotransferase (hph gene. Oil palm explants were bombarded under the following conditions: rupture disk to macrocarrier distance, 11 mm; macrocarrier to target tissue, 90 mm and using 1 μm gold particles as microcarrier. Four different pressures of helium were tested with three types of target tissues (mature embryo, embryogenic callus and young seedlings. From the transformation efficiency, calli were much more efficiently transformed in the biolistic process compared with mature embryos and seedlings. A 100% transformation efficiency for DNA delivery into callus oil palm explants was obtained at 850 psi helium pressures, for embryos a maximum 81.8% efficiency required 850 psi and for seedlings a maximum 75.9% efficiency required 1,550 psi. Using a confocal laser scanning microscope, and appropriate filters to block out the red fluorescence of chlorophyll, expression of the GFP gene was observed in all three bombarded explant types by a bright-green fluorescence. The mgfp5 gene was still present more than 8 months after bombardment, hence it indicated the stability of transgene in those transformants.

  5. Pendugaan Cadangan Karbon Above Ground Biomass (AGB) Pada Tegakan Sawit (Elaeis guineensis Jacq.)di Kabupaten Langkat

    Purba, Kepler Dopler

    2015-01-01

    Global warming is caused by the increasing of carbon consentration in the atmosphere beyond its natural consentration. One of the efforts to reduce greenhouse gases is by presenting plants or plants which absorb carbon. The information about the carbon content of the vegetation or forest stands can be obtained by guessing vegetation biomass. The technology of remote sensing and Geographic Information Systems are considered adequate to give the required information quickly and completely with ...

  6. Evaluasi Karakter Pertumbuhan Beberapa Varietas Kelapa Sawit (Elaeis guineensis Jacq.) di Pre Nursery Pada Beberapa Komposisi Media Tanam Tanah Gambut

    Lubis, Ade Moriza

    2014-01-01

    Palm oil is one of the plantations of Indonesia which has a bright future. Limitations of causing extensive agricultural productive land leads to marginal lands. Peatlands are one of the selected type of marginal land is land that rich in organic matter and nutrient poor. To achive good growth and productivity of the plant material must have a high genetic potential to be developed on marginal lands. The study aims to determine growth of several varieties of palm oil on peat soil compositi...

  7. Illegitimacy and sibship assignments in oil palm (Elaeis guineensis Jacq.) half-sib families using single locus DNA microsatellite markers.

    Hama-Ali, Emad Omer; Alwee, Sharifah Shahrul Rabiah Syed; Tan, Soon Guan; Panandam, Jothi Malar; Ling, Ho Chai; Namasivayam, Parameswari; Peng, Hoh Boon

    2015-05-01

    Oil palm breeding has been progressing very well in Southeast Asia, especially in Malaysia and Indonesia. Despite this progress, there are still problems due to the difficulty of controlled crossing in oil palm. Contaminated/illegitimate progeny has appeared in some breeding programs; late and failure of detection by the traditional method causes a waste of time and labor. The use of molecular markers improves the integrity of breeding programs in perennial crops such as oil palm. Four half-sib families with a total of 200 progeny were used in this study. Thirty polymorphic single locus DNA microsatellites markers were typed to identify the illegitimate individuals and to obtain the correct parental and progeny assignments by using the CERVUS and COLONY programs. Three illegitimate palms (1.5%) were found, and 16 loci proved to be sufficient for sibship assignments without parental genotypes by using the COLONY program. The pairwise-likelihood score (PLS) method was better for half-sib family assignments than the full likelihood (FL) method. PMID:25399079

  8. In vitro growth of Ganoderma boninense isolates on novel palm extract medium and virulence on oil palm (Elaeis guineensis seedlings

    Kok, S.M.

    2013-01-01

    Full Text Available Aims: Basal stem rot (BSR disease caused by Ganoderma pathogenic fungi, especially Ganoderma boninense is thriving rapidly in both areas with coastal and inland soils. The objectives of this study were to isolate and characterize Ganoderma isolates collected from various locations in Peninsular Malaysia through the comparison of their growth rate in vitroly on conventional and novel palm extract media, and to determine the degree of virulence caused by the isolatesin oil palm seedlings. Methodology and results: In this study, 12 Ganoderma isolates were collected from infected oil palm trees, fromvarious locations – Johor, Negeri Sembilan, Kedah, Perak, Pahang, and Kelantan, in Malaysia in year 2011. Twelve Ganoderma isolates were identified using molecular method with primer set that targeted at small-subunit 18S rDNA fragment, and characterized by determining the in vitro growth rate, and degree of virulence in 2-month-old oil palmseedlings in the nursery using both disease incidence (DI and disease severity index (DSI as the measurements to quantify the infection. All the Ganoderma isolates were identified as G. boninense and sequences of the respective isolates were deposited in GenBank. In general, all the isolates proliferated faster on oil palm extract medium (OPEM compared to malt extract agar (MEA. Twelve G. boninense isolates were observed to illustrate different degree of virulence ranging from highly pathogenic to least pathogenic. Conclusion, significance and impact of study: Cultures of 12 G. boninense isolates were observed to show faster growth rate (P < 0.014 on OPEM under in vitro conditions compared to conventional MEA medium, except Bt Lintang G10 and GBA G12 isolates. OPEM medium could provide a better alternative for maintaining and culturing Ganoderma strains. In the current study, both DI and DSI were highly correlated. However, there were low linear relationships (R2 < 0.423 between mycelia growth rate (on MEA and OPEM and degree of virulence (DI and DSI at 12-, 14- and 16-weeks after treatments among the G. boninense isolates tested. Furthermore, different degrees of virulence in twelve separate Ganoderma isolates were reported. Therefore, it is crucial to incorporate more than one isolate into any researches on screening for Ganoderma resistance or tolerance planting materials, searching for potential biological control agents, and studying bitrophic or tri-trophic interactions. In addition, this study was aimed to isolate G. boninense strains with various virulence levels for future studies.

  9. Camera-Vision Based Oil Content Prediction for Oil Palm (Elaeis Guineensis Jacq Fresh Fruits Bunch at Various Recording Distances

    Dinah Cherie

    2015-01-01

    Full Text Available In this study, the correlation between oil palm fresh fruits bunch (FFB appearance and its oil content (OC was explored. FFB samples were recorded from various distance (2, 7, and 10 m with different lighting spectrums and configurations (Ultraviolet: 280-380nm, Visible: 400-700nm, and Infrared: 720-1100nm and intensities (600watt and 1000watt lamps to explore the correlations. The recorded FFB images were segmented and its color features were subsequently extracted to be used as input variables for modeling the OC of the FFB. In this study, four developed models were selected to perform oil content prediction (OCP for intact FFBs. These models were selected based on their validity and accuracy upon performing the OCP. Models were developed using Multi-Linear-Perceptron-Artificial-Neural-Network (MLP-ANN methods, employing 10 hidden layers and 15 images features as input variables. Statistical engineering software was used to create the models. Although the number of FFB samples in this study was limited, four models were successfully developed to predict intact FFB’s OC, based on its images’ color features. Three OCP models developed for image recording from 10 m under UV, Vis2, and IR2 lighting configurations. Another model was successfully developed for short range imaging (2m under IR2 light. The coefficient of correlation for each model when validated was 0.816, 0.902, 0.919, and 0.886, respectively. For bias and error, these selected models obtained root-mean-square error (RMSE of 1.803, 0.753, 0.607, and 1.104, respectively.

  10. Simulating potential growth and yield of oil palm (Elaeis guineensis) with PALMSIM: Model description, evaluation and application.

    Hoffmann, Maik; Castañeda Vera, Alba; Van Wijk, M. T.; Giller, K.E.; Oberthür, T.; Donough, C.; Whitbread, A.M.

    2014-01-01

    Reducing the gap between water-limited potential yield and actual yield in oil palm production systems through intensification is seen as an important option for sustainably increasing palm oil production. Simulation models can play an important role in quantifying water-limited potential yield, and therefore the scope for intensification, but no oil palm model exists that is both simple enough and at the same time incorporates sufficient plant physiological knowledge to be generally applicab...

  11. Simulating potential growth and yield of oil palm (Elaeis guineensis) with PALMSIM: Model description, evaluation and application

    Hoffmann, M.; Castaneda Vera, A.; Wijk, van M.T.; Giller, K.E.; Oberthür, T.; Donough, C.; Whitbread, A.M.

    2014-01-01

    Reducing the gap between water-limited potential yield and actual yield in oil palm production systems through intensification is seen as an important option for sustainably increasing palm oil production. Simulation models can play an important role in quantifying water-limited potential yield, and

  12. Potential of Oil Palm Empty Fruit Bunch (EFB) as Fertilizer in Oil Palm (Elaeis guineensis L Jacq.) Nurseries

    ADEOLUWA, DR OLUGBENGA O.; ADEOYE, PROFESSOR GIDEON O.

    2008-01-01

    Oil palm is one of the major oil crops in the world. Oil palm empty fruit bunch (EFB) could serve as an alternative and cheaper organic fertilizer in oil palm farms. This study investigated the value of composts of different forms of EFB for raising oil palm in the nursery. The experiment, which covered the pre-nursery (< 3 months) and nursery stages (3-13 months) used different EFB: cow dung ratios (100:0, 90:10, 80:20, 70:30 and 60:40) as compost as well as cow dung only and mineral fertili...

  13. THE QUALITY OF COMPOSITE BOARD MADE OF WASTE OIL PALM STEM (Elaeis guineensis Jacq AND RECYCLE POLYETHYLENE (PE

    Maryam Jamilah Lubis, Iwan Risnasari, Arif Nuryawan, Fauzi Febrianto

    2001-04-01

    Full Text Available The main objective of this research was to find out the substitution of solid wood and it may solve environmental problem. These researches concerned on using waste of oil palm stem particle form and recycle plastic polyetyhlene (PE as raw materials for composite board. In this research, 5% Maleic Anhydride (MAH was added to increase compatibility and 0.75% Dicumyl Peroxide (DCP was added to initiate the reaction of maleolation. The methods of this research followed JIS A 5908 (2003 with ratio of plastic to particle were 50:50, 60:40 and 70:30, respectively, pressed at 30 kgf/cm2 in 165°C for 15 minutes. Evaluation on physical and mechanical properties based on JIS A 5908 (2003, and the result of physical properties as follow : 1 Density met the standard at 0.77 - 0.99 g/cm, 2 The value of moisture content were below on target and the standard because of the hidrofobicity of PE, the range were 0.79 - 3.06%, 3 Thickness swelling of the board for 24 hours fulfill the standard the value were 0.44 - 2.77%. Unfortunatelly the water absorption were 2.82 - 16.19%. Mechanical properties consist of modulus elasticity (MOE, modulus rupture (MOR and screw holding strength didn’t meet the criteria of JIS A 5908 (2003, except MOR with plastic: particle 60:40 with particle made of inner stem. Keywords: Composite board, oil palm stem, polyethylene (PE

  14. Pembuatan Selulosa Asetat Dari a-Selulosa Yang Diisolasi Dari Tandan Kosong Kelapa Sawit (Elaeis Guineensis Jack)

    Harahap, Mahyuni

    2013-01-01

    Making of cellulose acetate of -cellulose isolated from palm empty fruit bunches have been done. Palm empty fruit bunches delegnificationed and precipitated with 17,5% NaOH. Cellulose acetate is obtained through a process of esterification and hydrolysis -cellulose. The water content of celluloce acetate results at 0,042% and 20,01% of the acetyl content. FTIR analysis show that absorption characteristic of C=O carbonyl group at wave number 1724,36 cm-1 (cellulose acetate) and 1743,15 cm-1 ...

  15. Spatial variability of some soil properties in west coastal area of India having oil palm (Elaeis guineensis Jacq.) plantations

    Behera, Sanjib K.; Suresh, K.; Rao, B. N.; Mathur, Ravi K.; Shukla, Arvind K.; K Manorama; Ramachandrudu, K.; Harinarayana, P.

    2016-01-01

    Mapping spatial variability of soil properties is the key to efficient soil resource management for sustainable crop yield in coastal areas. Therefore, the present study was conducted to assess the spatial variability of soil properties like–acidity (pH), salinity (Electrical Conductivity (EC)), organic carbon, available K, available P, exchangeable Ca2+, exchangeable Mg2+, available S and hot wate...

  16. Spatial variability of some soil properties varies in oil palm (Elaeis guineensis Jacq.) plantations of west coastal area of India

    Behera, Sanjib Kumar; Suresh, Kancherla; Narsimha Rao, Bezawada; Mathur, Ravi Kumar; Shukla, Arvind Kumar; Manorama, Kamireddy; Ramachandrudu, Kummari; Harinarayana, Parasa; Prakash, Chandra

    2016-06-01

    Mapping spatial variability of soil properties is the key to efficient soil resource management for sustainable crop yield. Therefore, the present study was conducted to assess the spatial variability of soil properties such as acidity (pH), salinity (electrical conductivity (EC)), organic carbon, available K, available P, exchangeable Ca2+, exchangeable Mg2+, available S and hot water soluble B in surface (0-20 cm) and subsurface (20-40 cm) soil layers of oil palm plantations in south Goa district of Goa located in west coastal area of India. A total of 128 soil samples were collected from 64 oil palm plantations of Goa located at an approximate interval of 1-2 km and analyzed. Soil was acidic to neutral in reaction. Other soil properties varied widely in both the soil layers. Correlations between soil pH and exchangeable Ca2+, between soil EC and available K, between available P and available S and between exchangeable Ca2+ and exchangeable Mg2+ in both the soil layers were found to be positive and significant (P fit models for measured soil properties were exponential, Gaussian, stable, K-Bessel and spherical with moderate to strong spatial dependency. The results revealed that site-specific fertilizer management options needed to be adopted in the oil palm plantations of the study area owing to variability in soil properties.

  17. mRNA Expression of EgCHI1, EgCHI2, and EgCHI3 in Oil Palm Leaves (Elaeis guineesis Jacq. after Treatment with Ganoderma boninense Pat. and Trichoderma harzianum Rifai

    Laila Naher

    2012-01-01

    Full Text Available Background. Basal stem rot (BSR disease caused by the fungus Ganoderma boninense is the most serious disease affecting the oil palm; this is because the disease escapes the early disease detection. The biocontrol agent Trichoderma harzianum can protect the disease only at the early stage of the disease. In the present study, the expression levels of three oil palm (Elaeis guineensis Jacq. chitinases encoding EgCHI1, EgCHI2, and EgCHI3 at 2, 5, and 8 weeks inoculation were measured in oil palm leaves from plants treated with G. boninense or T. harzianum alone or both. Methods. The five-month-old oil palm seedlings were treated with Gano-wood blocks inoculum and trichomulch. Expression of EgCHI1, EgCHI2, and EgCHI3 in treated leaves tissue was determined by real-time PCR. Results. Oil palm chitinases were not strongly expressed in oil palm leaves of plants treated with G. boninense alone compared to other treatments. Throughout the 8-week experiment, expression of EgCHI1 increased more than 3-fold in leaves of plants treated with T. harzianum and G. boninense when compared to those of control and other treated plants. Conclusion. The data illustrated that chitinase cDNA expression varied depending on tissue and the type of treatment.

  18. Effect of replanting systems on populations of strategus aloeus (l.) and rhynchophorus palmarum (l.) associated with the oil palm oxg interspecific hybrid (elaeis oleifera × elaeis guineensis) in southwestern colombia

    Ávila, Rodrigo Andrés; Bayona, Cristhian; Ricón, Álvaro; Romero, Hernán Mauricio

    2014-01-01

    The oil palm replanting process produces a large amount of organic matter that, as it begins to decompose, becomes a breeding ground for various pests, including Strategus aloeus and Rhynchophorus palmarum. Different crop replanting systems are being used today. The method used depends on the plantation and it is basically associated with the costs involved in the process. However, sometimes too little attention is paid to other issues related to the new crop, such as plant health and agronom...

  19. Pengaruh Pemberian ZPT Dekamon 22,43 L Dan Pupuk Majemuk Totaro Terhadap Pertumbuhan Bibit Kelapa Sawit (Elaeis guineensis jacq) di Pembibitan Utama

    Sander, Alex

    2012-01-01

    Penelitian ini dilakukan di kebun percobaan Fakultas Pertanian UISU Kecamatan Medan Johor dengan ketinggian 27 meter di atas permukaan laut. Penelitian ini berlangsung dari bulan Januari - Mei 2000. Rancangan yang dilakukan adalah Rancangan Acak Lengkap (RAL) dengan 2 faktor.

  20. Comparative mineral and hormonal analyses of wild type and TLS somaclonal variant derived from oil palm (Elaeis guineensis Jacq. var. tenera) tissue culture

    Habib, S. H.; Ooi, S. E.; Novák, Ondřej; Tarkowská, Danuše; Rolčík, Jakub; Doležal, Karel; Syed-Alwee, S. S. R.; Ho, C. L.; Namasivayam, P.

    2012-01-01

    Roč. 68, č. 2 (2012), s. 313-317. ISSN 0167-6903 Grant ostatní: GA MŠk(CZ) ED0007/01/01 Institutional research plan: CEZ:AV0Z50380511 Keywords : truncated leaf syndrome * boron * zinc Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.670, year: 2012

  1. Pengaruh ZPT Dekamon 22,43 L dan Pupuk Pelengkap Cair TNF terhadap Pertumbuhan Bibit Kelapa Sawit (Elaeis guineensis Jacq) di Pernbibitan Utama

    Flato Genius S

    2012-01-01

    Penelitian ini elilakukan di kebun percobaan Fakultas Pertanian UISU Kecamatan Medan Johor dengan ketinggian 27 meter diatas permukaan laut. Penelitian ini berlangsung dari bulan Mei -September 2000. Rancangan yang digunakan dalam penelitian ini adalah rancangan Acak Lengkap (RAL) dengan 2 faktor.

  2. EFFECT OF MICRONUTRIENTS-ENRICHED FERTILIZERS ON BASAL STEM ROT DISEASE INCIDENCE AND SEVERITY ON OIL PALM (ELAEIS GUINEENSIS JACQ. SEEDLINGS

    Fabien Fonguimgo Tengoua

    2014-01-01

    Full Text Available Basal stem rot caused by Ganoderma boninense constitutes a serious threat to oil palm industry in Southeast Asia, especially in Malaysia and Indonesia and in Papua New Guinea and Pacific Islands. It is also expanding in some oil palm growing countries in Latin America and Africa and will soon become a worldwide concern to oil palm cultivation. To date, none of the various control measures developed and tested to control the disease since many decade gives entire satisfaction. An experiment was carried out to see whether incorporation of micronutrients, Copper (Cu, Boron (B and Manganese (Mn could reduce the incidence and severity of this disease on oil palm seedlings inoculated with G. boninense. The concentrations tested were 2 mg B/kg of soil, 2 mg Cu/kg of soil and 2 mg Mn/kg of soil incorporated into the basic fertilizer NPKMg 14-10-10-2. Treatments were applied in solution for three months before inoculation, followed by soil application for eight months after inoculation. The results showed that although no significant difference was detected among treatments, the double combinations of these micronutrients, B+Cu, B+Mn and Cu+Mn, performed better than the single nutrients in reducing the incidence and the severity of BSR, while their triple combination rather increased these pathological parameters. These double combinations could therefore be field-tested for their further integration in oil palm fertilization programme.

  3. Respons Pertumbuhan Bibit Kelapa Sawit (Elaeis guineensis Jacq.) Terhadap Pemberian Kompos Sampah Pasar dan Pupuk NPKMg (15:15:6:4) di Pre Nursery

    Tambunan, Martua Markus

    2014-01-01

    The research was conducted at the experimental field Agricultural Faculty, The Sumatera Utara University about ± 25 metres above sea level from April to July 2014. The design use randomized block design with 2 factors treatment. The first factor was The waste market compost with 4 levels: 0 g/polybag (K0);50 g/polybag (K1); 100 g/polybag (K2); 150 g/polybag (K3) and the second factor was NPKMg (15:15:6:4) fertilizers: 0 g/polybag P0); 2,5 g/polybag (P1); 5 g/polybag (P2) and 7,5 g/polybag...

  4. Pertumbuhan Bibit Kelapa Sawit (Elaeis guineensis Jacq.)pada Berbagai Perbandingan Media Tanam Sludge dan Tandan Kosong Kelapa Sawit (TKKS) di Pre Nursery

    Nasution, Haikal Hanif

    2015-01-01

    The objective of this research was to study growth of oil palm seedlings at various growing media comparisons of sludge and EPOEFB in pre nursery. This research was conducted atKebun Bangun PTPN III from January-June 2013 using non- factorial randomized block design consisted of 11 types of growing media: ultisol topsoil, sludge, TKKS and composite media 10:80:10, 10:70:20, 10:60:30, 10:50:40, 10:40:50, 10:30:60, 10:20:70, 10:10:80. Parameters observed wereplant height, number of chlorophyll,...

  5. Pendugaan Cadangan Karbon Pada Tegakan Kelapa Sawit (Elaeis guineensis Jacq.) Umur 15 Tahun di Perkebunan Kelapa Sawit Putri Hijau, Besitang Sumatera Utara

    Sitanggang, Guswinda Nasari

    2015-01-01

    Forests absorb CO2 during photosynthesis and store it as organic matter in biomass plants, as well as oil palm plantations. This study aimed were to : (1) obtain a model Allometric estimation of carbon stocks in vegetation potential of oil palm plantations in North Sumatra; (2) to obtain the potential carbon stocks in forest conversion to oil palm plantations in North Sumatra. This research was conducted in the area of Oil Palm Putri Hijau, Besitang North Sumatra. The research was carried out...

  6. Tingkat Serangan Ulat Kantong Metisa plana Walker (Lepidoptera: Psychidae) terhadap Umur Tanaman Kelapa Sawit (Elaeis guineensis Jacq.) di Kebun Matapao PT Socfin Indonesia

    Sembiring, Nugraha

    2015-01-01

    The goal this research was to know the incidence level of the bagworm against the age of oil palm in Matapao Estate on the produce plant or non produce plant. The research was carried out at Matapao Estate PT Socfin Indonesia, Kecamatan Teluk Mengkudu, Kabupaten Serdang Bedagai with place height ± 20 m on surface of sea. The research use Survey method with diagonal sample. The result showed that the incidence level of bagworm on non produce plant was bigger than onn produce plant. The l...

  7. Efecto de la alimentación con lodo de palma (Elaeis guineensis sobre la producción de leche

    Ramón Zambrano Morán

    2016-01-01

    Full Text Available La escasez de alimento para el ganado vacuno en la época de pocas lluvias ocasiona la pérdida de peso y producción en vacas lac-tantes. Este estudio pretende demostrar la viabilidad de reemplazar la pastura por lodo de palma durante los períodos de escasez manteniendo los niveles de producción de leche y carne de las vacas lactantes. Para esto se contó con cinco tratamientos donde se adicionó 2, 5, 8 y 11 kg de lodo de palma más un tratamiento sin adición (control por 60 días. Durante este lapso de tiempo se registró el peso de la leche y del animal cada tres días. Para la variable peso del animal se obtuvo que el mejor y peor tratamiento corresponde al control y 11 kg de lodo de palma respectivamente; para la variable producción de leche el mejor tratamiento corresponde a 11 kg de lodo de palma y el peor tratamiento es el control. Se concluye que el tratamiento que presenta una buena relación de peso del animal y producción de leche corresponde a las vacas alimentadas con 5 kg de lodo de palma ya que ambas variables se ubican como segundo mejor tratamiento con una variación de 11% y 9,50% respectivamente.

  8. In vitro somatic embryogenesis and plantlet regeneration from immature male inflorescence of adult dura and tenera palms of Elaeis guineensis (Jacq.)

    Jayanthi, Madhavan; Susanthi, Bollarapu; Murali Mohan, Nandiganti; Mandal, Pranab Kumar

    2015-01-01

    We report here a method for plant regeneration through somatic embryogenesis from explants collected from immature male inflorescence of adult oil palm cultivated in India. Callus induction was successful from tissues of immature male inflorescence collected from both dura and tenera varieties of oil palm. A modified Y3 (Eeuwens) media supplemented with several additives and activated charcoal (3%) were used for the experiments. Out of four different auxin treatments, 4-amino-3,5,6-trichloro-...

  9. Peningkatan Sifat Mekanik Lembaran Nanokomposit Berbasis Lateks Pekat Karet Alam (Hevea Brasiliensis) Yang Diperkuat Oleh Nanokristal Selulosa Dari Tandan Kosong Kelapa Sawit (Elaeis Guineensis Jack) Dengan Teknik Pencelupan

    Putri, Elisa

    2016-01-01

    Research about manufacturing of concentrated latex-based natural rubbernanocomposites sheetsusing nanocrystalline cellulose (NCC) isolated from a Palm Oil Empty BunchesPalm (POEBP) as areinforcing agenthas been done. The production of the nanocomposite sheetswereprepared through three stages: isolation of α-cellulose from POEBP, continued with isolation of NCC from α-cellulose using H2SO4 48,84%, and the manufacture of nanocomposite sheetwith various concentration of NCCof0, 1, 2, 3, 4, and ...

  10. Same-vessel enzymatic saccharification and fermentation of organosolv/H2O2 pretreated oil palm (Elaeis guineensis Jacq.) fronds for bioethanol production: Optimization of process parameters

    Highlights: • Same vessel enzymatic saccharification and fermentation (SVSF) of pretreated OPFs. • Optimum conditions:37 °C, 8.0% solid loading, 14.0 g/l yeast concentration, pH 5.3. • Optimum bioethanol concentration and yield of 21.96 g/l and 84.65% respectively. • Organosolv/H2O2 pretreatment of OPFs improved SVSF yield at high solid loading. - Abstract: Based on optimized pretreatment process, oil palm fronds (OPFs) were sequentially pretreated with 1.4% (w/v) aq. NaOH in 80% ethanol with ultrasound assistance (at 75 °C for 30 min) and 3% (v/v) aq. H2O2. Using the Box–Behnken design (BBD) of response surface methodology (RSM), bioethanol production from the sono-assisted organosolv/H2O2 OPFs were optimized using same-vessel enzymatic saccharification and fermentation (SVSF) where both the hydrolysis and fermentation processes were carried out in one vessel simultaneously. Throughout the SVSF process, the incubation time and enzyme loading were kept at 72 h and 15 filter paper unit (FPU)/g substrate respectively. The other SVSF parameters which affect bioethanol yield such as temperature (X1: 30–50 °C), solid loading (X2: 5.0–10.0% w/v), yeast concentration (X3: 5.0–20 g/l) and pH (X4: 4.0–7.0) were optimized. Well fitted regression equations (R2 > 0.97) obtained were able to predict reliable optimum bioethanol concentration and yield. The predicted optimum bioethanol concentration (i.e., 20.61 g/l) and yield (i.e., 84.60%) were attained at 36.94 °C (∼37 °C), 7.57% w/v solid loading (∼8.0% w/v), 13.97 g/l yeast concentration (∼14.0 g/l) and pH of 5.29 (∼5.30). Validated results indicated a maximum ethanol concentration and yield of 21.96 g/l and 84.65% respectively, which were closer to the predicted optimum responses. Using the optimum conditions, the highest bioethanol productivity of 0.76 g/l/h was observed at 12 h of SVSF process