WorldWideScience

Sample records for accompanies pro-thymocyte proliferation

  1. Anisomycin-induced GATA-6 degradation accompanying a decrease of proliferation of colorectal cancer cell.

    Ushijima, Hironori; Horyozaki, Akiko; Maeda, Masatomo

    2016-09-01

    Transcription factor GATA-6 plays a key role in normal cell differentiation of the mesoderm and endoderm. On the other hand, GATA-6 is abnormally overexpressed in many clinical gastrointestinal cancer tissue samples, and accelerates cell proliferation or an anti-apoptotic response in cancerous tissues. We previously showed that activation of the JNK signaling cascade causes proteolysis of GATA-6. In this study, we demonstrated that anisomycin, a JNK activator, stimulates nuclear export of GATA-6 in a colorectal cancer cell line, DLD-1. Concomitantly, anisomycin remarkably inhibits the proliferation of DLD-1 cells via G2/M arrest in a plate culture. However, it did not induce apoptosis under growth arrest conditions. Furthermore, the growth of DLD-1 cells in a spheroid culture was suppressed by anisomycin. Although 5-FU showed only a slight inhibitory effect on 3D spheroid cultures, the same concentration of 5-FU together with a low concentration of anisomycin exhibited strong growth inhibition. These results suggest that the induction of GATA-6 dysfunction may be more effective for chemotherapy for colorectal cancer, although the mechanism underlying the synergistic effect of 5-FU and anisomycin remains unknown. PMID:27404124

  2. Psychosocial Accompaniment

    Mary Watkins

    2015-01-01

    This essay advocates for a paradigm shift in psychology toward the activity and ethics of accompaniment. Accompaniment requires a reorientation of the subjectivity, interpersonal practices, and critical understanding of the accompanier so that (s)he can stand alongside others who desire listening, witnessing, advocacy, space to develop critical inquiry and research, and joint imagination and action to address desired and needed changes. The idea of “accompaniment” emerged in liberation theolo...

  3. Proliferation risks

    The report gives an overview of different aspects related to safeguards of fissile materials. Existing treaties including the Non-Proliferation Treaty, and the Tlatelolco and the Rarotonga Treaties are discussed. An overview of safeguards systems for the control of fissile materials as well as the role of various authorities is given. An overall overview of proliferation risks, the physical protection of fissile materials and the trade in fissile materials is given. Finally, the status in problem countries and de facto nuclear weapon states is discussed

  4. 19 CFR 148.4 - Accompanying articles.

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Accompanying articles. 148.4 Section 148.4 Customs... (CONTINUED) PERSONAL DECLARATIONS AND EXEMPTIONS General Provisions § 148.4 Accompanying articles. (a) Generally. Articles shall be considered as accompanying a passenger or brought in by him if the...

  5. Handling Proliferation

    Pierre Laszlo

    2001-10-01

    Full Text Available The ethics of the chemist identify with those of the citizen, in principle. The observed perversions, such as proliferation of chemicals, stem from the values of a chemical community closed upon itself, and from the attendant identification of a mere know-how with a science. The epistemic degradation produces moral indifference.

  6. Metastatic Basal Cell Carcinoma Accompanying Gorlin Syndrome

    Yeliz Bilir; Erkan Gokce; Banu Ozturk; Faik Alev Deresoy; Ruken Yuksekkaya; Emel Yaman

    2014-01-01

    Gorlin-Goltz syndrome or basal cell nevus syndrome is an autosomal dominant syndrome characterized by skeletal anomalies, numerous cysts observed in the jaw, and multiple basal cell carcinoma of the skin, which may be accompanied by falx cerebri calcification. Basal cell carcinoma is the most commonly skin tumor with slow clinical course and low metastatic potential. Its concomitance with Gorlin syndrome, resulting from a mutation in a tumor suppressor gene, may substantially change morbidity...

  7. Novice Collaboration in Solo and Accompaniment Improvisation

    Hansen, Anne-Marie; Andersen, Hans Jørgen

    2012-01-01

    This study investigates how non-musicians engaged in a solo-accompaniment music improvisation relationship. Seven user teams interacted with two electronic music instruments integrated in two pen tablets. One instrument was a melody instrument and the other a chord instru-ment. The study was done in order to understand how future shared electronic music instruments can be de-signed to encourage non-musicians to engage in social action through music improvisation. A combination of quantitative...

  8. Solutions manual to accompany Fundamentals of calculus

    Morris, Carla C

    2015-01-01

    Solutions Manual to Accompany Fundamentals of Calculus the text that encourages students to use power, quotient, and product rules for solutions as well as stresses the importance of modeling skills.  In addition to core integral and differential calculus coverage, the core book features finite calculus, which lends itself to modeling and spreadsheets.  Specifically, finite calculus is applied to marginal economic analysis, finance, growth, and decay.  Includes: Linear Equations and Functions The Derivative Using the Derivative Exponential and Logarithmic

  9. Severe Hypoglycemia Accompanied with Thyroid Crisis

    Yuki Nakatani

    2012-01-01

    Full Text Available We report a 32-year-old Japanese women with severe hypoglycemia accompanied with thyroid crisis. She complained of dyspnea, general fatigue, and leg edema. She was diagnosed with hyperthyroidism with congestive heart failure and liver dysfunction. Soon after admission, sudden cardiopulmonary arrest occurred. She was then transferred to the intensive care unit. Her serum glucose level was 7 mg/dl. Intravenous glucose, hydrocortisone, diuretics, and continuous hemodiafiltration (CHDF saved her. We considered that hypoglycemia occurred due to heart failure and liver dysfunction due to thyroid crisis.

  10. Novice Collaboration in Solo and Accompaniment Improvisation

    Hansen, Anne-Marie; Andersen, Hans Jørgen

    2012-01-01

    in order to understand how future shared electronic music instruments can be de-signed to encourage non-musicians to engage in social action through music improvisation. A combination of quantitative and qualitative analysis was used to find characteristics in co-expression found in a solo......This study investigates how non-musicians engaged in a solo-accompaniment music improvisation relationship. Seven user teams interacted with two electronic music instruments integrated in two pen tablets. One instrument was a melody instrument and the other a chord instru-ment. The study was done...

  11. Tumefactive Brain Demyelination Accompanying MADSAM Neuropathy

    Şefik Evren Erdener

    2015-09-01

    Full Text Available Multifocal acquired demyelinating sensory and motor (MADSAM neuropathy is characterized by asymmetric multifocal motor and sensory loss and conduction blocks in peripheral nerves. Peripheral demyelinating diseases may be accompanied by demyelination in central nervous system (CNS. In this report, a MADSAM patient with a solitary tumefactive demyelinating lesion in brain is presented. Neuroimaging due to a visual field defect revealed a right parietooccipital lesion, which was initially misdiagnosed as a tumor. Pathological examination showed that it was demyelinating in nature. Peripheral nervous symptoms of the patient developed two years later and she was then diagnosed with MADSAM. There was prominent clinical and electrophysiological response to steroid treatment. Tumefactive brain involvement was not previously reported for MADSAM neuropathy, although it was documented in a single case with typical chronic inflammatory demyelinating polyneuropathy (CIDP. CNS involvement should therefore be considered in MADSAM patients.

  12. Sacral perineural cyst accompanying disc herniation.

    Ju, Chang Il; Shin, Ho; Kim, Seok Won; Kim, Hyeun Sung

    2009-03-01

    Although most of sacral perineural cysts are asymptomatic, some may produce symptoms. Specific radicular pain may be due to distortion, compression, or stretching of nerve root by a space occupying cyst. We report a rare case of S1 radiculopathy caused by sacral perineural cyst accompanying disc herniation. The patient underwent a microscopic discectomy at L5-S1 level. However, the patient's symptoms did not improved. The hypesthesia persisted, as did the right leg pain. Cyst-subarachnoid shunt was set to decompress nerve root and to equalize the cerebrospinal fluid pressure between the cephalad thecal sac and cyst. Immediately after surgery, the patient had no leg pain. After 6 months, the patient still remained free of leg pain. PMID:19352483

  13. Bilateral foveal retinoschisis accompanying unilateral peripheral retinoschisis

    Nilufer Kocak

    2014-01-01

    Full Text Available X-linked juvenile retinoschisis is a rare hereditary retinal disease characterized by a tangential splitting of the neurosensory retina which may cause early-onset visual impairment. Existence of the retinal neurosensory layer splitting on cross-sectional images of optical coherance tomography (OCT and the absence of leakage on fluorescein angiography (FA help confirming the diagnosis. Such diagnostic tests are also helpful in determining the management of the disease. However, most of the retinoschisis cavities remain stable and rarely extend to the posterior pole, many authors suggest laser prophylaxis to avoid the potential risk of retinal detachment due to holes in the outer retinal layer. Herein, we report a case with bilateral foveal retinoschisis accompanying unilateral peripheral retinoschisis who was evaluated with detailed ophthalmologic examination. Visual acuity, fundoscopy, OCT, and FA remained stable in the second year of follow-up after prophylactic argon laser treatment.

  14. Proliferation: myth or reality?

    This article analyzes the proliferation approach, its technical condition and political motivation, and the share between the myth (political deception, assumptions and extrapolations) and the reality of proliferation. Its appreciation is complicated by the irrational behaviour of some political actors and by the significant loss of the non-use taboo. The control of technologies is an important element for proliferation slowing down but an efficient and autonomous intelligence system remains indispensable. (J.S.)

  15. Auroral pulsations and accompanying VLF emissions

    V. R. Tagirov

    Full Text Available Results of simultaneous TV observations of pulsating auroral patches and ELF-VLF-emissions in the morning sector carried out in Sodankylä (Finland on February 15, 1991 are presented. Auroral pulsating activity was typical having pulsating patches with characteristic periods of about 7 s. Narrow-band hiss emissions and chorus elements at intervals of 0.3–0.4 s formed the main ELF-VLF activity in the frequency range 1.0–2.5 kHz at the same time. The analysis of auroral images with time resolution of 0.04 s allowed perfectly separate analysis of spatial and temporal variations in the auroral luminosity. Mutual correspondence between the behaviour of the luminous auroral patches and the appearance of ELF noise type hiss emissions and VLF chorus trains was found in two intervals chosen for analysis. While the hiss emissions were associated with the appearance of luminosity inside a limited area close to the zenith, the structured VLF emissions were accompanied by rapid motion of luminosity inside the area. The spatial dimension of the pulsating area was about 45–50 km and luminosity propagated inside it with velocity of about 10–12 kms. We discuss a new approach to explain the 5–15 s auroral pulsation based on the theory of flowing cyclotron maser and relaxation characteristics of ionosphere.

    Key words. Magnetospheric physics (auroral phenomena; magnetosphere-ionosphere interactions · Space plasma physics (wave-particle interactions

  16. Surgical complications accompanying liver transplantation in Estonia.

    Väli, T; Tein, A; Tikk, T; Sillakivi, T

    2010-12-01

    The purpose of this study was to evaluate surgical complications accompanying the introduction of orthotopic liver transplantation (OLT) in Estonia. Between 1999 and 2009, we performed the first 12 liver transplantations. Eight patients were males and four were females of age range 12 to 67 years. Their diagnoses were cholestatic disease (n = 5); tumor (n = 3); hepatitis C virus cirrhosis (n = 2); Budd-Chiari syndrome (n = 1); and cystic fibrosis (n = 1). Technical complications occurred in 7/12 patients. The early vascular complications in two patients were a suprahepatic vena cava lesion occurring at liver extraction, which resulted in uncontrolled suprahepatic bleeding after liver perfusion; the recipient died during transplantation. The other case displayed a right intrahepatic portal venous thrombosis, which was treated successfully with thrombolysis and anticoagulant therapy. Early biliary complications of biliary leaks occurred in three patients: two had undergone duct-to-duct reconstructions, which were treated by endoscopic retrograde cholangiography that successfully managed the anastomotic and recipient cystic duct leaks with a papillotomy and stenting. In one patient with a duct-to-jejunum anastomosis, a bile leak stopped at 3 weeks but he needed surgical therapy 2 years later due to an anastomotic stricture. Severe decubitus occurred in the lumbosacral region of the subjects with operating times of 14 hours. They required necretectomy and plastic surgery. One of them with postoperative intra-abdominal hypertension also displayed wound eventration requiring reoperations. The rate of hepatic (5/12) and extrahepatic (3/12) surgical complications, as well as of 1-year survival (9/12), in our period of implementation of OLT were satisfactory to continue OLT development in Estonia. PMID:21168717

  17. 16 CFR 1500.125 - Labeling requirements for accompanying literature.

    2010-01-01

    ... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Labeling requirements for accompanying literature. 1500.125 Section 1500.125 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL... REGULATIONS § 1500.125 Labeling requirements for accompanying literature. When any accompanying...

  18. Director's series on proliferation

    This is an occasional publication of essays on the topics of nuclear, chemical, biological, and missile proliferation. The views represented are those of the author's. Essay topics include: Nuclear Proliferation: Myth and Reality; Problems of Enforcing Compliance with Arms Control Agreements; The Unreliability of the Russian Officer Corps: Reluctant Domestic Warriors; and Russia's Nuclear Legacy

  19. Proliferation Networks and Financing

    The objective of this study is to propose practical solutions aimed at completing and strengthening the existing arrangement for the control of nuclear proliferation through a control of financial as well as material or immaterial flows. In a first part, the author proposes a systemic analysis of networks of suppliers and demanders. He notably evokes the Khan's network and the Iraqi acquisition network during the 1993-2001 period. He also proposes a modelling of proliferation networks (supplier networks and acquisition networks) and of their interactions. In a second part, the author examines possible means and policies aimed at neutralising proliferation networks: organisation, adaptation and improvement of intelligence tools in front of proliferation networks, and means, limitations and perspectives of network neutralisation. He also briefly addresses the possibility of military action to contain proliferation flows

  20. Getting serious about proliferation

    The US needs to give a higher priority to nuclear non-proliferation, but Reagan's policies assume that proliferation is inevitable and that it is more important to be a reliable supplier than to cause trade frictions by trading only with those nations which sign the non-proliferation treaty (NPT). This undercuts US leadership and the intent of the agreement. Several bills now before Congress could help to restore US leadership by tightening export restrictions and the use of plutonium from the US

  1. 9 CFR 93.208 - Articles accompanying poultry.

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Articles accompanying poultry. 93.208 Section 93.208 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF... FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Poultry § 93.208 Articles accompanying poultry....

  2. 9 CFR 93.508 - Articles accompanying swine.

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Articles accompanying swine. 93.508 Section 93.508 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF... FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Swine § 93.508 Articles accompanying swine. No...

  3. 9 CFR 93.409 - Articles accompanying ruminants.

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Articles accompanying ruminants. 93.409 Section 93.409 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT...; REQUIREMENTS FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Ruminants § 93.409 Articles accompanying...

  4. 9 CFR 93.307 - Articles accompanying horses.

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Articles accompanying horses. 93.307... FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Horses § 93.307 Articles accompanying horses. No..., blankets, or other things used for or about horses governed by the regulations this part, shall be...

  5. Harmonizing Melody with Meta-Structure of Piano Accompaniment Figure

    Yin Feng; Kui Chen; Xiang-Bin Liu

    2011-01-01

    In this paper,a meta-structure of piano accompaniment figure (meta-structure for short) is proposed to harmonize a melodic piece of music so as to construct a multi-voice music.Here we approach melody harmonization with piano accompaniment as a machine learning task in a probabilistic framework.A series of piano accompaniment figures are collected from the massive existing sample scores and converted into a set of meta-structure.After the procedure of samples training,a model is formulated to generate a proper piano accompaniment figure for a harmonizing unit in the context.This model is flexible in harmonizing a melody with piano accompaniment.The experimental results are evaluated and discussed.

  6. The nuclear proliferation; La proliferation nucleaire

    Gere, F. [Ecole Polytechnique, 91 - Palaiseau (France)

    1995-04-01

    In this book is detailed the beginning of nuclear military power, with the first bomb of Hiroshima, the different ways of getting uranium 235 and plutonium 239, and how the first countries (Usa, Ussr, China, United kingdom, France) got nuclear weapons. Then the most important part is reviewed with the details of non-proliferation treaty and the creation of IAEA to promote civilian nuclear power in the world and to control the use of plutonium and uranium in nuclear power plants. The cases of countries who reached the atom mastery, such Israel, South Africa, Pakistan, Iraq, North Korea, Argentina, Brazil, Iran, Algeria, Taiwan and the reasons which they wanted nuclear weapon for or why they gave up, are exposed.

  7. Cell Proliferation in Neuroblastoma

    Laura L. Stafman

    2016-01-01

    Full Text Available Neuroblastoma, the most common extracranial solid tumor of childhood, continues to carry a dismal prognosis for children diagnosed with advanced stage or relapsed disease. This review focuses upon factors responsible for cell proliferation in neuroblastoma including transcription factors, kinases, and regulators of the cell cycle. Novel therapeutic strategies directed toward these targets in neuroblastoma are discussed.

  8. Battling Nuclear Proliferation

    2007-01-01

    As the North Korean and Iranian nuclear issues develop and efforts to resolve them continue, global attention to anti-nuclear proliferation and the work of the International Atomic Energy Agency (IAEA) has become even more intense. Pang Sen, Chairman of

  9. Critical thinking of student nurses during clinical accompaniment

    BY Uys; SM Meyer

    2005-01-01

    The purpose of this study was to investigate the methods of clinical accompaniment used by clinical facilitators in practice. The findings of the study also reflected facilitators’ perceptions regarding critical thinking and the facilitation thereof. A quantitative research design was used. A literature study was conducted to identify the methods of accompaniment that facilitate critical thinking. Data was collected by means of a questionnaire developed for that purpose. Making a content-rela...

  10. A Case of Cluster Headache Accompanied by Myoclonus and Hemiparesis

    Yang, Ji Won; Park, Suk Gyung; Jung, In Hae; Sung, Young Hee; Park, Kee Hyung; Lee, Yeong Bae; Shin, Dong Jin; Park, Hyeon Mi

    2012-01-01

    Background Cluster headache is a primary headache disorder characterized by periodic episodes of intense headache accompanied by autonomic symptoms. We report an unusual clinical presentation of cluster headache that was preceded by myoclonus and accompanied by hemiparesis. Case Report A 26-year-old man visited hospital due to recurrent jerky movements on the left side of his face and neck area lasting 3 days. These jerky movements had disappeared spontaneously without specific treatment. On ...

  11. Correlation between thyroid function and nodular goiter accompanied with gallstone

    Shihong Ma; Qinjiang Liu; Xiaofeng Hou

    2014-01-01

    The-purpose-of-the-study-wass-to-explore-the-correlation-between-thyroid-function-and-nodular-goiter-accompanied-with-gal-stone.-Methods:We-col-ected-120-cases-about-nodular-goiter-accompanied-with-gal-stone-and-128-cases-about-nodular-goiter-and-establish-50-healthy-control-groups.-Detected-t-level-of-hyrotropic-hormone-(TSH),-total-tri-o-dothyronine-(TT3),-total-thyroxine-in-the-peripheral-venous-blood-of-these-cases-in-the-three-groups-by-using-electrochemilu-minescence-immunoassay,-measure-level-of-total-cholesterol-(TC),-high-density-lipoprotein-cholesterol-(HDL-C),-low-density-lipoprotein-cholesterol-(LDL-C)-and-total-bile-acid-(TBA)-levels-by-using-enzymic-method,-and-observed-the-changes-of-thyroid-function-and-blood-lipid-among-the-three-groups.-Results:The-serum-TT3-level-in-nodular-goiter-accompanied-with-gal-stone-group-and-the-nodular-goiter-group-was-significantly-lower-than-that-in-control-group-(P0.05).-Accordingly,-TC-and-LDL-C-level-in-nodular-goiter-accompanied-with-gal-stone-group-was-significantly-higher-than-that-in-nodular-goiter-and-control-group-(P0.05).-The-HDL-C-level-in-nodular-goiter-accompanied-with-gal-stone-group-and-control-group-was-higher-than-that-in-simple-nodular-goiter-group-(P<0.01).-Conclusion:The-originating-etiologic-factor-of-nodular-goiter-accompanied-with-gal-stone-may-be-related-to-that-the-decreased-TT3-induced-sub-clinical-hypothyroidism.

  12. Non-proliferation considerations

    This paper reiterates the Indian viewpoint that consideration of ''proliferation resistance'' is outside the terms of reference of Working Group 4 as agreed at the Washington Conference. The discussions in WG4 should therefore cover only safeguards aspects. The paper goes on to critisize the various assessment factors introduced in INFCE/DEP./WG-4/104 and the various alternative technologies proposed. The Indian view is reinstated that if a country requires reprocessing based on its nuclear energy programmes and priorities, there should be no hindrance. International safeguards should be applied to all nuclear materials in all countries without discrimination or differentiation between civil and military programmes. The paper concludes that non-proliferation is essentially a political matter and has no technical solution

  13. Dexamethasone and Salbutamol Stimulate Human Lung Fibroblast Proliferation

    Pickholtz, Eran; Admon, Dan; Izhar, Uzi; Berkman, Neville; Levi-Schaffer, Francesca

    2011-01-01

    Background Asthma is characterized by bronchial hyperreactivity and airway remodeling. Subepithelial fibrosis, a feature of remodeling, is accompanied by activation of fibroblasts to myofibroblasts, with excessive proliferation and increased collagen, extracellular matrix protein, and profibrogenic cytokine production. Mast cells are important in the development of asthma and its fibrotic changes. Objective In this study, we aimed to investigate the direct effect of the drugs most frequently ...

  14. A Rare Cutaneous Adnexal Tumor: Malignant Proliferating Trichilemmal Tumor

    Omer Alici; Musa Kemal Keles; Alper Kurt

    2015-01-01

    Proliferating trichilemmal tumors (PTTs) are neoplasms derived from the outer root sheath of the hair follicle. These tumors, which commonly affect the scalp of elderly women, rarely demonstrate malignant transformation. Although invasion of the tumors into neighboring tissues and being accompanied with anaplasia and necrosis are accepted as findings of malignancy, histological features may not always be sufficient to identify these tumors. The clinical behavior of the tumor may be incompatib...

  15. Proliferation after the Iraq war

    This article uses the Iraq war major event to analyze the approach used by the US to fight against proliferation. It questions the decision and analysis process which has led to the US-British intervention and analyzes the consequences of the war on the proliferation of other countries and on the expected perspectives. Finally, the future of proliferation itself is questioned: do we have to fear more threat or is the virtuous circle of non-proliferation well started? (J.S.)

  16. Can we predict nuclear proliferation

    The author aims at improving nuclear proliferation prediction capacities, i.e. the capacities to identify countries susceptible to acquire nuclear weapons, to interpret sensitive activities, and to assess nuclear program modalities. He first proposes a retrospective assessment of counter-proliferation actions since 1945. Then, based on academic studies, he analyzes what causes and motivates proliferation, with notably the possibility of existence of a chain phenomenon (mechanisms driving from one program to another). He makes recommendations for a global approach to proliferation prediction, and proposes proliferation indices and indicators

  17. Non-proliferation

    The issue of Nuclear Non Proliferation has been moved to a leading place on the contemporary international security agenda. What about the situation of nuclear weapons and nuclear technology in Russia, Kazakhstan, Ukraine and Belorussia? Why did the IAEA-inspectors totally failed to discover any sign of Iraq's clandestine nuclear-weapon programme before the Gulf War? Do the NATO and their nuclear power states violate Art. VI of the Non-Proliferation-Treaty (NPT), because they are - despite the end of the cold war - not willing to renounce of the ''option of the first use of nuclear weapons''? Does the NPT establish a form of nuclear apartheid? What will be the situation if the NPT-Extension-Conference in 1995 will be unable to obtain a majority of the parties for any one extension proposal? Do we need a new international nuclear control agency with severe powers, a sort of nuclear Interpol? The Colloquium ''Saving NPT and abolishing Nuclear Weapons'', held in Stockholm in September 1992, organized by the Swedish and the German Sections of IALANA, tried to analyse some of the raised issues. (orig.)

  18. Non Proliferation of Nuclear

    Non-Proliferation Treaty of Nuclear Weapons is the international community's efforts to maintain the security of the world, in order to prevent the spread of nuclear technology and the use of nuclear weapons, promoting cooperation for the use of nuclear peaceful purposes, build mutual trust (Confidence Building Measures) as well as to achieve the ultimate goal of disarmament overall (General and Complete Disarmament). Addressing the post-WTC tragedy, 11 September 2001, the Indonesian government should set up a National Measures (National Action Plan), among others formed the National Security Council and NBC Counter Proliferation Unit, or the National Authority for Nuclear Treaty, preparing national legislation, to prevent the abuse nuclear materials for terrorist acts, prevent Illicit Trafficking of Nuclear materials, developed a National Preparedness and Emergency Response Management in the event of a nuclear accident or attack by the use of nuclear terrorism. Importance of a National Action Plan meant the existence of a national commitment in the context of compliance with treaties and conventions which have been ratified relating to safety, security, safeguards towards a general and complete disarmament, to safeguard national security and maintain peace (safeguards) international

  19. Initiatives for proliferation prevention

    Preventing the proliferation of weapons of mass destruction is a central part of US national security policy. A principal instrument of the Department of Energy's (DOE's) program for securing weapons of mass destruction technology and expertise and removing incentives for scientists, engineers and technicians in the newly independent states (NIS) of the former Soviet Union to go to rogue countries or assist terrorist groups is the Initiatives for Proliferation Prevention (IPP). IPP was initiated pursuant to the 1994 Foreign Operations Appropriations Act. IPP is a nonproliferation program with a commercialization strategy. IPP seeks to enhance US national security and to achieve nonproliferation objectives by engaging scientists, engineers and technicians from former NIS weapons institutes; redirecting their activities in cooperatively-developed, commercially viable non-weapons related projects. These projects lead to commercial and economic benefits for both the NIS and the US IPP projects are funded in Russian, Ukraine, Kazakhstan and Belarus. This booklet offers an overview of the IPP program as well as a sampling of some of the projects which are currently underway

  20. Initiatives for proliferation prevention

    NONE

    1997-04-01

    Preventing the proliferation of weapons of mass destruction is a central part of US national security policy. A principal instrument of the Department of Energy`s (DOE`s) program for securing weapons of mass destruction technology and expertise and removing incentives for scientists, engineers and technicians in the newly independent states (NIS) of the former Soviet Union to go to rogue countries or assist terrorist groups is the Initiatives for Proliferation Prevention (IPP). IPP was initiated pursuant to the 1994 Foreign Operations Appropriations Act. IPP is a nonproliferation program with a commercialization strategy. IPP seeks to enhance US national security and to achieve nonproliferation objectives by engaging scientists, engineers and technicians from former NIS weapons institutes; redirecting their activities in cooperatively-developed, commercially viable non-weapons related projects. These projects lead to commercial and economic benefits for both the NIS and the US IPP projects are funded in Russian, Ukraine, Kazakhstan and Belarus. This booklet offers an overview of the IPP program as well as a sampling of some of the projects which are currently underway.

  1. Uncertainties in Nuclear Proliferation Modeling

    There have been various efforts in the research community to understand the determinants of nuclear proliferation and develop quantitative tools to predict nuclear proliferation events. Such systematic approaches have shown the possibility to provide warning for the international community to prevent nuclear proliferation activities. However, there are still large debates for the robustness of the actual effect of determinants and projection results. Some studies have shown that several factors can cause uncertainties in previous quantitative nuclear proliferation modeling works. This paper analyzes the uncertainties in the past approaches and suggests future works in the view of proliferation history, analysis methods, and variable selection. The research community still lacks the knowledge for the source of uncertainty in current models. Fundamental problems in modeling will remain even other advanced modeling method is developed. Before starting to develop fancy model based on the time dependent proliferation determinants' hypothesis, using graph theory, etc., it is important to analyze the uncertainty of current model to solve the fundamental problems of nuclear proliferation modeling. The uncertainty from different proliferation history coding is small. Serious problems are from limited analysis methods and correlation among the variables. Problems in regression analysis and survival analysis cause huge uncertainties when using the same dataset, which decreases the robustness of the result. Inaccurate variables for nuclear proliferation also increase the uncertainty. To overcome these problems, further quantitative research should focus on analyzing the knowledge suggested on the qualitative nuclear proliferation studies

  2. Proliferation: myth or reality?; La proliferation: mythe ou realite?

    NONE

    2005-07-01

    This article analyzes the proliferation approach, its technical condition and political motivation, and the share between the myth (political deception, assumptions and extrapolations) and the reality of proliferation. Its appreciation is complicated by the irrational behaviour of some political actors and by the significant loss of the non-use taboo. The control of technologies is an important element for proliferation slowing down but an efficient and autonomous intelligence system remains indispensable. (J.S.)

  3. Bilateral retinal cyst accompanying with renal and thyroidal multiorgan cyst

    Amber Şenel; Rıfat Rasier; Alper Şengül; Erdal Yüzbaşıoğlu; Özgür Artunay; Halil Bahçecioğlu

    2011-01-01

    Although retinal cysts are commonly seen in von Hippel Lindau syndrome, bilateral retinal cysts can occasionally appear unrelated to this syndrome. Retinal cysts can be confused with retinal tumours and infectious diseases of retina. In this report we represent a patient demonstrating bilateral retinal cyst accompanying with renal and thyroidal multiorgan cysts without Von-Hippel Lindau syndrome.

  4. Dosimetric accompaniment of high technology radiation therapy. Instruction for use

    The contents of prepared guide on high technological radiation dosimetric accompaniment is discussed in scope of application of modern linear electron accelerator. The main attention is paid on dose measurements types for evaluation quality work of separate systems and devices used for patients irradiation

  5. Lateral Medullary Infarction and Accompanying Sustained Hiccup: Treatment with Gabapentin

    BERİLGEN, M Said; DEMİR, Caner Feyzi; AYDIN, Meliha; Erdoğan, Ercan

    2007-01-01

    Hiccup, which is a symptom seen during lateral medullary syndrome, appears due to sudden contraction of diaphragma and external (inspiratory) intercostal muscles and the closure of glottis. Here we present a case with lateral medullary infarction accompanied with chronic hiccup, the dramatical improvement with gabapentine and the probable related pathophysiologic mechanism.. ©2007, Firat University, Medical Faculty

  6. Food Service: Handbook to Accompany VESL Vocabulary Cards.

    Taylor, Nancy; Lenhart, Debra

    This manual is one of four self-contained components of a larger handbook designed to assist secondary and postsecondary instructors and support staff in meeting the needs of limited-English-proficiency (LEP) students in vocational training programs. Together with an accompanying set of vocational English as a second language (VESL) vocabulary…

  7. Critical thinking of student nurses during clinical accompaniment

    BY Uys

    2005-09-01

    Full Text Available The purpose of this study was to investigate the methods of clinical accompaniment used by clinical facilitators in practice. The findings of the study also reflected facilitators’ perceptions regarding critical thinking and the facilitation thereof. A quantitative research design was used. A literature study was conducted to identify the methods of accompaniment that facilitate critical thinking. Data was collected by means of a questionnaire developed for that purpose. Making a content-related validity judgment, and involving seven clinical facilitators in an academic institution, ensured the validity of the questionnaire. The results of the study indicated that various clinical methods of accompaniment were used. To a large extent, these methods correlated with those discussed in the literature review. The researcher further concluded that the concepts ‘critical thinking’ and ‘facilitation’ were not interpreted correctly by the respondents, and would therefore not be implemented in a proper manner in nursing practice. Furthermore, it seemed evident that tutor-driven learning realised more often than student-driven learning. In this regard, the requirement of outcomes-based education was not satisfied. The researcher is therefore of the opinion that a practical programme for the development of critical thinking skills during clinical accompaniment must be developed within the framework of outcomes-based education.

  8. Accompanied driving in The Netherlands : who do participate and why?

    Schagen, I.N.L.G. van Wijlhuizen, G.J. & Craen, S. de

    2015-01-01

    In 2011, a six year accompanied driving experiment started in the Netherlands. This questionnaire study investigated which youngsters (intend to) participate and why, with the purpose of assessing whether there is a potential self-selection bias that can be relevant for the oncoming evaluation of it

  9. 9 CFR 93.314 - Horses, certification, and accompanying equipment.

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Horses, certification, and... PRODUCTS; REQUIREMENTS FOR MEANS OF CONVEYANCE AND SHIPPING CONTAINERS Horses § 93.314 Horses, certification, and accompanying equipment. (a) Horses offered for importation from any part of the world...

  10. 29 CFR 575.5 - Supporting data to accompany application.

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Supporting data to accompany application. 575.5 Section 575.5 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS WAIVER OF CHILD LABOR PROVISIONS FOR AGRICULTURAL EMPLOYMENT OF 10 AND 11 YEAR OLD MINORS IN...

  11. The changing proliferation threat

    Sopko, J.F.

    1996-12-31

    Technological advances and new adversaries with new motives have reduced the relevancy and effectiveness of the American nonproliferation strategy that was developed during the Cold War. The Cold War`s end and the breakup of the Soviet Union have created new proliferation dangers even as they have reduced others. The familiar balance of nuclear terror that linked the superpowers and their client states for nearly 50 years in a choreographed series of confrontations has given way to a much less predictable situation, where weapons of unthinkable power appear within the grasp of those more willing to use them. Rogue nations and {open_quotes}clientless{close_quotes} states, terrorist groups, religious cults, ethnic minorities, disaffected political groups, and even individuals appear to have jointed a new arms race toward mass destruction. The author describes recent events that suggest the new trends and a serious challenge to US national security.

  12. Problems with radiation protection concerning volunteers accompanying radiological patients

    Full text: The purpose of this work is to point out, within the framework of the Radiation Protection guidelines, the irregular situation of the 'volunteer' or 'accompanying person' who accompanies anyone requiring medical treatment with ionising radiation, as well as to suggest a possible justification for such role. It should be noted that most of these persons are subject to ionising radiation without knowing anything about the effects that it could cause on them, so that their condition could be hardly considered as 'voluntary'. There are several circumstances under which the presence of accompanying persons is required, being different among them. Several examples could be mentioned such as: those who are accompanying a direct relative (family bonds), those who are acting in service during their normal work (social workers, policemen) and even those who are forced by unusual under an accidental situation. The qualitative classification that radiological protection established in society concerning radiation risks for people in general enables to set mechanisms of justification, optimisation and dose limitation for each category, being perfectly identified which of them each person belongs to. But the figure of 'accompanying person' has been excluded from such characterisation. They are subject to radiation exposure without knowing it, or without having any information concerning the potential risks. For them, no balance between the net benefit of an adequate medical treatment versus potential health detriment may be applied as for the case of a patient. Thus, their exposure could be not justified. It is not the purpose of this work to question radiological medicine or its practices, but to clarify certain aspects involving members of the public in general, patients and members of the radiological community, as well as to propose lines of action concerning this subject. We conclude that it is not the volunteer who should decide about medical actions, a role

  13. Accompaniment needs of nursing students related to the dying patient

    D van Rooyen; Laing, R.; WJ Kotzé

    2005-01-01

    Nurse educators are responsible for accompanying students towards becoming capable, competent professional nurses who are a credit to themselves, their patients, colleagues and profession. Student nurses need, therefore, to be taught to render comprehensive nursing care to patients in all stages of their lives, including when they are dying. Being confronted with human suffering and death is challenging and traumatic. Those exposed to such events on a daily basis need to have a solid foundati...

  14. Visual Messages Accompanied by Music: Preschool Children’s Interpretations

    Dogani, Konstantina; Constandinidou-Semoglou, Ourania

    2016-01-01

    Within current visual culture children are continuously exposed to varieties of visual messages accompanied by music which could contribute towards their aesthetic development. However, the interaction between music and image is not always as simple as in animation where music has direct relationship to the visual message to be easily interpreted by the preschool child. Taking a semiotic approach the current paper investigates preschool child’s reception of a more sophisticated interaction be...

  15. The Raven,a Loner’s Psychological Accompanier

    商滔

    2015-01-01

    Edgar Allan Poe’s The Raven is well-known to the world for its detailed psychological descriptions in the author’s eyes of this essay.Whereas,the Raven is the only one accompanie and the best listener listening to Poe’s psychological cry for his lover.The author will try to present the scene of the sadness from Poe by employing Carl G.Jung’s theories about consciousness and unconsciousness.

  16. The Raven,a Loner’s Psychological Accompanier

    商滔

    2015-01-01

    Edgar Allan Poe’s The Raven is well-known to the world for its detailed psychological descriptions in the author’s eyes of this essay.Whereas,the Raven is the only one accompanier and the best listener listening to Poe’s psychological cry for his lover.The author will try to present the scene of the sadness from Poe by employing Carl G.Jung’s theories about consciousness and unconsciousness.

  17. Real-time implementation of an interactive jazz accompaniment system

    Deshpande, Nikhil

    Modern computational algorithms and digital signal processing (DSP) are able to combine with human performers without forced or predetermined structure in order to create dynamic and real-time accompaniment systems. With modern computing power and intelligent algorithm layout and design, it is possible to achieve more detailed auditory analysis of live music. Using this information, computer code can follow and predict how a human's musical performance evolves, and use this to react in a musical manner. This project builds a real-time accompaniment system to perform together with live musicians, with a focus on live jazz performance and improvisation. The system utilizes a new polyphonic pitch detector and embeds it in an Ableton Live system - combined with Max for Live - to perform elements of audio analysis, generation, and triggering. The system also relies on tension curves and information rate calculations from the Creative Artificially Intuitive and Reasoning Agent (CAIRA) system to help understand and predict human improvisation. These metrics are vital to the core system and allow for extrapolated audio analysis. The system is able to react dynamically to a human performer, and can successfully accompany the human as an entire rhythm section.

  18. Accompaniment needs of nursing students related to the dying patient

    D Van Rooyen

    2005-09-01

    Full Text Available Nurse educators are responsible for accompanying students towards becoming capable, competent professional nurses who are a credit to themselves, their patients, colleagues and profession. Student nurses need, therefore, to be taught to render comprehensive nursing care to patients in all stages of their lives, including when they are dying. Being confronted with human suffering and death is challenging and traumatic. Those exposed to such events on a daily basis need to have a solid foundation of self preservation to see past the pain of suffering and to bring light and hope to those in need. A young student nurse will only experience positive growth and development in these circumstances if she is also cared for and guided with understanding. The researcher utilized a qualitative, explorative, descriptive and contextual design based on the phenomenological approach to enquiry. The following question was asked at the beginning of each unstructured phenomenological interview: “How was if for you to care for a dying or deceased patient?” The central theme identified that student nurses experience turmoil in their different relationships in their accompaniment of the dying patient. Guidelines based on the central theme and sub-themes that emerged from raw data, as well as literature, are offered as strategies to promote/enhance optimal accompaniment of student nurses caring for the dying patient.

  19. Isotopic yield in alpha accompanied ternary fission of 252Cf

    The cold ternary fission of 252Cf with 4He as light charged particle (LCP) is studied with fragments in the equatorial and collinear configuration, taking the interacting barrier as the sum of the Coulomb and proximity potential. The favorable fragment combinations are obtained from the cold valley plot and by calculating the yield for charge-minimized fragments. In both equatorial and collinear configurations, the highest yield is obtained for the fragment combination 116Pd + 4He + 132Sn, which possess doubly magic nuclei 132Sn(N = 82, Z = 50). The presence of doubly or near doubly magic nuclei (132Sn, 130Sn etc.) and higher Q value plays an important role in the alpha accompanied cold ternary fission of 252Cf. The comparison of the relative yield for equatorial configuration with that of collinear configuration, points to the fact that equatorial configuration is the preferred configuration for the LCP (4He) accompanied ternary fission in 252Cf isotope. The yields obtained for the alpha accompanied cold ternary fission of 252Cf in equatorial and collinear configuration are compared with the experimental data. The emission probability of long range alpha (LRA) particle from 252Cf isotope is predicted using our formalism and is found to be in agreement with experimental value. (author)

  20. Nuclear proliferation: linkages and solutions

    Nuclear proliferation must be periodically re-examined as a moral as well as a practical foreign policy dilemma. The question is asked whether proliferation precludes a safe and peaceful world, or if a halt to proliferation is adequate without other arms control. The moral dilemma in foreign policy arises over the need to make practical choices which often serve one goal while sacrificing another. The ramifications of nuclear proliferation are examined and the conclusions reached that it is not an acceptable option. It is also decided that, because general disarmament steps will be more difficult to achieve, the world may have to accept a small number of nuclear arsenals as the price of state sovereignties. A high priority for making the effort to prevent proliferation is advised. 8 references

  1. Activating Effect of Benzbromarone, a Uricosuric Drug, on Peroxisome Proliferator-Activated Receptors

    Chiyoko Kunishima

    2007-01-01

    Full Text Available Benzbromarone, a uricosuric drug, reportedly causes hepatic hypertrophy accompanied by proliferation of peroxisomes in rats. To elucidate the mechanisms underlying induction of peroxisome proliferation by benzbromarone, we examined binding affinity for peroxisome proliferator-activated receptor α (PPARα and γ (PPARγ, and effects on the binding activity of PPARs with peroxisome proliferation-responsive element (PPRE and expression of the PPARs target protein. Binding affinity of benzbromarone for PPARα and PPARγ was examined by reporter gene assay. Binding activity of PPARs with PPRE was determined by electric mobility shift assay, and expression of lipoprotein lipase (LPL and acyl-CoA synthetase (ACS by Western blot method. Benzbromarone displayed affinity for PPARα and PPARγ, and promoted binding of PPARs to PPRE. Furthermore, cultured cells with benzbromarone added showed upregulated expression of LPL and ACS. These results suggest that benzbromarone induces peroxisome proliferation in hepatocytes by binding to PPARs, and controls expression of proteins related to lipid metabolism.

  2. Activating effect of benzbromarone, a uricosuric drug, on peroxisome proliferator-activated receptors.

    Kunishima, Chiyoko; Inoue, Ikuo; Oikawa, Toshihiro; Nakajima, Hiromu; Komoda, Tsugikazu; Katayama, Shigehiro

    2007-01-01

    Benzbromarone, a uricosuric drug, reportedly causes hepatic hypertrophy accompanied by proliferation of peroxisomes in rats. To elucidate the mechanisms underlying induction of peroxisome proliferation by benzbromarone, we examined binding affinity for peroxisome proliferator-activated receptor alpha (PPARalpha) and gamma (PPARgamma), and effects on the binding activity of PPARs with peroxisome proliferation-responsive element (PPRE) and expression of the PPARs target protein. Binding affinity of benzbromarone for PPARalpha and PPARgamma was examined by reporter gene assay. Binding activity of PPARs with PPRE was determined by electric mobility shift assay, and expression of lipoprotein lipase (LPL) and acyl-CoA synthetase (ACS) by Western blot method. Benzbromarone displayed affinity for PPARalpha and PPARgamma, and promoted binding of PPARs to PPRE. Furthermore, cultured cells with benzbromarone added showed upregulated expression of LPL and ACS. These results suggest that benzbromarone induces peroxisome proliferation in hepatocytes by binding to PPARs, and controls expression of proteins related to lipid metabolism. PMID:18274627

  3. Solutions manual to accompany finite mathematics models and applications

    Morris, Carla C

    2015-01-01

    A solutions manual to accompany Finite Mathematics: Models and Applications In order to emphasize the main concepts of each chapter, Finite Mathematics: Models and Applications features plentiful pedagogical elements throughout such as special exercises, end notes, hints, select solutions, biographies of key mathematicians, boxed key principles, a glossary of important terms and topics, and an overview of use of technology. The book encourages the modeling of linear programs and their solutions and uses common computer software programs such as LINDO. In addition to extensive chapters on pr

  4. Mucocele Accompanied by a Traumatic Neuroma: A Case Report

    Jaafari Ashkavandi Z.

    2013-03-01

    Full Text Available Mucocele and traumatic neuroma are two lesions related to the traumatic events; however there is only one reported case in which these two entities were perceived simultaneously. The current study reported a 21-year-old man who complained of painless recurrent swelling, accompanied by paresthesia on his left lower labial mucosa. He had a previous history of similar lesion and had been treated with surgery and cauterization last year. The primary clinical impression was a recurrent mucocele. Microscopic surveys displayed a traumatic neuroma in the vicinity of a mucocele which seems to be arising from the previous surgical treatment.

  5. Marfan Syndrome Accompanied by Pituitary Adenomas: A Case Report

    Qi HUANG

    2014-04-01

    Full Text Available Marfan syndrome (MFS is a dominant inherited connective tissue disease. It has variable clinical manifestations and affects a number of systems, such as the cardiovascular system, eyes, skeletal, lung, skin etc. Fibrillin-1(FBN1)gene mutations are found in approximately 90% patients. The abnormal proteins originated from the mutant gene play a dominant negative effect on normal proteins or change their susceptibility of protease, which leads to the change of connective tissue. This paper reports the clinical data of a patient with Marfan syndrome accompanied by pituitary adenomas and reviews related literatures at home and abroad, to explore its pathogenesis.

  6. Exoemission accompanying the decomposition of methanol on zinc oxide

    Krylova, I. V.

    2008-09-01

    The electronic phenomena accompanying the adsorption and dehydrogenation of methanol on zinc oxide were studied using the method of exoemission of negative charges. Postemission excited from ZnO by an electron beam was found to be suppressed by the adsorption of methanol vapor, which exhibited electron acceptor properties. Subsequent heating to temperatures close to the temperature of the beginning of methanol decomposition increased the intensity of exoemission, which was evidence of the participation of emission centers (Oδ-) in dehydrogenation. A possible mechanism of methanol decomposition with the participation of surface V s hole centers (Oδ-) of zinc oxide was suggested.

  7. Study guide to accompany computers data and processing

    Deitel, Harvey M

    1985-01-01

    Study Guide to Accompany Computer and Data Processing provides information pertinent to the fundamental aspects of computers and computer technology. This book presents the key benefits of using computers.Organized into five parts encompassing 19 chapters, this book begins with an overview of the evolution of modern computing systems from the earliest mechanical calculating devices to microchips. This text then introduces computer hardware and describes the processor. Other chapters describe how microprocessors are made and describe the physical operation of computers. This book discusses as w

  8. Test bank to accompany Computers data and processing

    Deitel, Harvey M

    1980-01-01

    Test Bank to Accompany Computers and Data Processing provides a variety of questions from which instructors can easily custom tailor exams appropriate for their particular courses. This book contains over 4000 short-answer questions that span the full range of topics for introductory computing course.This book is organized into five parts encompassing 19 chapters. This text provides a very large number of questions so that instructors can produce different exam testing essentially the same topics in succeeding semesters. Three types of questions are included in this book, including multiple ch

  9. Nuclear non proliferation and disarmament

    In the framework of the publication of a document on the ''weapons mastership, disarmament and non proliferation: the french action'', by the ministry of Foreign Affairs and the ministry of Defense, the French Documentation organization presents a whole document. This document describes and details the following topics: the conference on the treaty of non proliferation of nuclear weapons, the France, Usa and Non Governmental Organizations position, the threats of the proliferation, the french actions towards the disarmament, the disarmament in the world, a chronology and some bibliographic resources. (A.L.B.)

  10. RNA interference targeting raptor inhibits proliferation of gastric cancer cells

    Mammalian target of rapamycin complex 1 (mTORC1) is dysregulated in gastric cancer. The biologic function of mTORC1 in gastric carcinogenesis is unclear. Here, we demonstrate that disruption of mTORC1 function by RNA interference-mediated downregulation of raptor substantially inhibited gastric cancer cell proliferation through induction of G0/G1-phase cell cycle arrest. The anti-proliferative effect was accompanied by concomitant downregulation of activator protein-1 and upregulation of Smad2/3 transcriptional activities. In addition, the expression of cyclin D3 and p21Waf1, which stabilizes cyclin D/cdk4 complex for G1-S transition, was reduced by raptor knockdown. In conclusion, disruption of mTORC1 inhibits gastric cancer cell proliferation through multiple pathways. This discovery may have an implication in the application of mTORC1-directed therapy for the treatment of gastric cancer.

  11. Behavior of Water Jet Accompanied with Air Suction

    Kawakami, Hironobu; Ishido, Tsutomu; Ihara, Akio

    In order to atomize a liquid, the authors have investigated the behavior of air-water jets. In a series of experiments, we have discovered a strange phenomenon that the water jet accompanied with air suction from the free surface has made a periodic radial splash of water drop. The purpose of the present paper is to clear out the origin of this phenomenon and the behavior of water jet accompanied with air suction. The behavior of water jet has been photographed by a digital camera aided with a flashlight and high-speed video camera. Those experiments enable us to find the origin of a periodic radial splash due to a formation of single air bubble at the flow separation region inside the nozzle and due to explosive expansion of the bubble after injected in the free space. In order to analyze the radial splash of water, we have conducted the equation of spherical liquid membrane. The numerical results obtained have been compared with the experimental results and good agreement has been obtained in radial expansion velocity.

  12. Value-sensitive clinical accompaniment in community nursing science

    Ebin Arries

    2010-03-01

    Full Text Available Clinical community health facilities where undergraduate students are placed for their practical work in community nursing science are dynamic and have undergone major transformation over the past few years. In the clinical field, community nurses and undergraduate students are representative of the different races and language and ethnic groups in the South African population, with each group espousing different value systems. Both parties – students and community nurses – report that, due to these differences, value conflicts are experienced during clinical accompaniment and that this has negative effects on clinical learning in community nursing science.

    The goal of this study was to explore and describe the experiences of students with regard to value-sensitive clinical accompaniment in the community nursing environment. An exploratory, descriptive and contextual design was used. Interactions between community nurses and students during clinical accompaniment were explored for value sensitivity by means of video recordings,participant observation and focus group interviews. Data were collected by means of video recordings, participant observation and focus group interviews. The data were analysed and coded by the researcher and the external coder, using an inductive descriptive method to identify important segments of the regularity of behaviour. The focus group interviews were transcribed, analysed and coded by the researcher and the external coder, using Tesch’s steps of analysis (Creswell 1994:155–156.Lincoln and Guba’s criteria (1985:290 for trustworthiness were applied to the study.

    The general findings indicate that clinical accompaniment in community nursing is not value sensitive and, as a result, guidelines for value-sensitive clinical accompaniment need to be developed for undergraduate students in the community nursing environment. The following values (values for which guidelines need to be developed were

  13. Spinal hydatidosis accompanied by a secondary infection. Case report.

    Kotil, Kadir; Tatar, Zeynep; Bilge, Turgay

    2007-06-01

    Spinal hydatid disease is rarely encountered in nonendemic regions. It is a progressive disease that is associated with risks of serious morbidity. The authors report a case of an isolated primary hydatid cyst of the spine in a 34-year-old woman who presented with back pain, which had lasted for 3 months, as well as lower-extremity pain and fatigue, which had persisted for 2 months. A neurological examination yielded findings indicative of upper motor neuron involvement with complete sensory loss below the level of T-11. Magnetic resonance imaging of the spine showed multiple extradural cystic lesions with an abscess formation at T-11 and involvement of the paraspinal muscles. The patient underwent spinal decompression in which stabilization and total excision of the multiple epidural and psoas abscesses and paraspinal multiloculated cysts were performed. The diagnosis of hydatid disease associated with another infection was confirmed by histopathological evaluation. Albendazole was administered during the postoperative period. Previous reports of secondary infections accompanying this disease in extraspinal locations have been published. However, to the best of the authors' knowledge, there have been no publications about a secondary infection associated with hydatidosis in the spinal cord. One should bear in mind that spinal hydatidosis may be accompanied by other infections in endemic regions. Antihelminthic treatment should be administered for a long period following early decompressive surgery and adequate stabilization. PMID:17561751

  14. Treatment of lingual traumatic ulcer accompanied with fungal infections

    Sella Sella

    2011-09-01

    Full Text Available Background: Traumatic ulcer is a common form of ulceration occured in oral cavity caused by mechanical trauma, either acute or chronic, resulting in loss of the entire epithelium. Traumatic ulcer often occurs in children that are usually found on buccal mucosa, labial mucosa of upper and lower lip, lateral tongue, and a variety of areas that may be bitten. To properly diagnose the ulcer, dentists should evaluate the history and clinical description in detail. If the lesion is allegedly accompanied by other infections, such as fungal, bacterial or viral infections, microbiological or serological tests will be required. One of the initial therapy given for fungal infection is nystatin which aimed to support the recovery and repair processes of epithelial tissue in traumatic ulcer case. Purpose: This case report is aimed to emphasize the importance of microbiological examination in suspected cases of ulcer accompanied with traumatic fungal infection. Case: A 12-year-old girl came to the clinic of Pediatric Dentistry, Faculty of Dentistry, University of Indonesia on June 9, 2011 accompanied with her mother. The patient who had a history of geographic tongue came with complaints of injury found in the middle of the tongue. The main diagnosis was ulcer accompanied with traumatic fungal infection based on the results of swab examination. Case management: This traumatic ulcer case was treated with Dental Health Education, oral prophylaxis, as well as prescribing and usage instructions of nystatin. The recovery and repair processes of mucosal epithelium of the tongue then occured after the use of nystatin. Conclusion: It can be concluded that microbiological examination is important to diagnose suspected cases of ulcer accompanied with traumatic fungal infection. The appropriate treatment such as nystatin can be given for traumatic fungal infection.Latar belakang: Ulkus traumatic merupakan bentuk umum dari ulserasi rongga mulut yang terjadi akibat trauma

  15. Cytosolic DNA Sensor Upregulation Accompanies DNA Electrotransfer in B16.F10 Melanoma Cells.

    Znidar, Katarina; Bosnjak, Masa; Cemazar, Maja; Heller, Loree C

    2016-01-01

    In several preclinical tumor models, antitumor effects occur after intratumoral electroporation, also known as electrotransfer, of plasmid DNA devoid of a therapeutic gene. In mouse melanomas, these effects are preceded by significant elevation of several proinflammatory cytokines. These observations implicate the binding and activation of intracellular DNA-specific pattern recognition receptors or DNA sensors in response to DNA electrotransfer. In tumors, IFNβ mRNA and protein levels significantly increased. The mRNAs of several DNA sensors were detected, and DAI, DDX60, and p204 tended to be upregulated. These effects were accompanied with reduced tumor growth and increased tumor necrosis. In B16.F10 cells in culture, IFNβ mRNA and protein levels were significantly upregulated. The mRNAs for several DNA sensors were present in these cells; DNA-dependent activator of interferon regulatory factor (DAI), DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 (DDX60), and p204 were significantly upregulated while DDX60 protein levels were coordinately upregulated. Upregulation of DNA sensors in tumors could be masked by the lower transfection efficiency compared to in vitro or to dilution by other tumor cell types. Mirroring the observation of tumor necrosis, cells underwent a significant DNA concentration-dependent decrease in proliferation and survival. Taken together, these results indicate that DNA electrotransfer may cause the upregulation of several intracellular DNA sensors in B16.F10 cells, inducing effects in vitro and potentially in vivo. PMID:27271988

  16. A case of multiple intracranial tuberculomas accompanied by tuberculous meningitis

    A 63-year-old man was admitted for headache and progressive drowsiness. In spite of negative results from cerebrospinal fluid (CSF) stains and cultures of tuberculosis and other bacteria, CSF findings and tuberculosis-positive sputum culture led to a tentative diagnosis of tuberculous meningitis. Symptoms and neurological test results both improved remarkably during antituberculous chemotherapy. Although the brain CT scan taken on admission showed no abnormal findings, one taken two months later revealed multiple high-density areas after enhancement with a contrast medium, suggesting multiple intracranial tuberculomas accompanied by tuberculous meningitis. The size of the multiple high-density areas gradually decreased during antituberculous chemotherapy. The CT scan can clearly locate lesions, detect complications, and evaluate the effects of treatment in patients with intracranial tuberculosis. (author)

  17. Sudden synchrony leaps accompanied by frequency multiplications in neuronal activity

    Ido Kanter

    2013-10-01

    Full Text Available A classical view of neural coding relies on temporal firing synchrony among functional groups of neurons; however the underlying mechanism remains an enigma. Here we experimentally demonstrate a mechanism where time-lags among neuronal spiking leap from several tens of milliseconds to nearly zero-lag synchrony. It also allows sudden leaps out of synchrony, hence forming short epochs of synchrony. Our results are based on an experimental procedure where conditioned stimulations were enforced on circuits of neurons embedded within a large-scale network of cortical cells in vitro and are corroborated by simulations of neuronal populations. The underlying biological mechanisms are the unavoidable increase of the neuronal response latency to ongoing stimulations and temporal or spatial summation required to generate evoked spikes. These sudden leaps in and out of synchrony may be accompanied by multiplications of the neuronal firing frequency, hence offering reliable information-bearing indicators which may bridge between the two principal neuronal coding paradigms.

  18. Intracranial hypotension accompanied by trauma. A prospective study in Japan

    To clarify the clinical characteristics of definite and non-definite diagnosis groups in intracranial hypotension accompanied by trauma (IHAT), we undertook investigations of registered patients (n=23) prospectively. The results revealed the following features of IHAT: definite diagnosis group, 4 cases; they had long periods from injury to onset (5.5±5.7 days), but these were markedly shorter than in the non-definite group; 75% of definite cases showed typical postural headache; 75% of definite cases showed dural enhancement on Gd-MRI; all 4 cases had confirmed cerebrospinal fluid (CFS) leakage based on imaging diagnosis with cervical lesions in 2 cases and cervical-thoracic lesions in 2 cases; and 6) good recovery was achieved in them by conservative therapy and blood patch, with a cure rate of 100%. These findings suggest that confirmed CFS leakage is indispensable for reaching definite diagnosis of IHAT. (author)

  19. Ectopic Ureter Accompanied by Duplicated Ureter: Three Cases.

    Senel, Ufuk; Tanriverdi, Halil Ibrahim; Ozmen, Zafer; Sozubir, Selami

    2015-09-01

    We report cases of ectopic ureter accompanied by three types of ureteral duplication that had been diagnosed previously and treated for enuresis. Data from three female patients ranging in age from 1 to 10 years were evaluated. The ectopic ureter was observed on the left in one case, on the right in another and bilateral in the third case. Complete duplication was found in two cases, while the third had incomplete duplication. Ureteroneocystostomy was performed in one case and subtotal nephrectomy was carried out in the other two cases. Ureteroneocystostomy was performed for the ectopic ureter found in the opposite urinary system in one of the cases. Ectopic duplicated ureter should be considered in treatment-resistant enuresis and urinary tract infections and after a careful physical examination, imaging as well as function tests should be performed. PMID:26500949

  20. MR imaging of edema accompanying benign and malignant bone tumors

    To evaluate the incidence, quantity, and presentation of intra- and extraosseous edema accompanying benign and malignant primary bone lesions, the magnetic resonance (MR) studies of 63 consecutive patients with histologically proven primary bone tumors were reviewed. MR scans were assessed for the presence and quantity of marrow and soft tissue edema and correlated with preoperative findings, resected specimens and follow-up data. The signal intensity and enhancement of tumor and edema prior to and after intravenous administration (if any) of gadolinium-labled diethylene triamine pentaacetate (Gd-DTPA) was analyzed. Marrow edema was encountered adjacent to 8 of 39 maglinant tumors and 14 of 24 benign lesions. Soft tissue edema was found accompanying 28 of 39 malignancies and 10 of 24 benign disorders. On enhanced T1-weighted MR images tumor and edema were difficult to differentiate. Tumor inhomogeneity made this differentiation easier on T2-weighted sequences. In 36 patients the contrast medium Gd-DTPA was used. Edema was present in 27 of these patients and the respective enhancement of tumor and edema could be compared. Edema always enhanced homogeneously, and in most cases it enhanced to a similar degree as or more than tumor. Marrow and, more specifically, soft tissue edema is a frequent finding adjacent to primary bone tumors. The mere presence and quantity of marrow and soft tissue edema are unreliable indicators of the biologic potential of a lesion. Unenhanced MR scans cannot always differentiate between tumor and edema, but the administration of Gd-DTPA is of assistance in differentiating tumor from edema. Awareness of marrow and/or soft tissue edema adjacent to bone lesions is of importance because edema can be a pitfall in the diagnostic work-up and staging prior to biopsy or surgery. (orig.)

  1. The human leukemia oncogene bcr-abl abrogates the anchorage requirement but not the growth factor requirement for proliferation.

    Renshaw, M W; McWhirter, J R; Wang, J Y

    1995-01-01

    Proliferation of normal cells in a multicellular organism requires not only growth factors but also the proper attachment to the extracellular matrix. A hallmark of neoplastic transformation is the loss of anchorage dependence which usually accompanies the loss of growth factor requirement. The Bcr-Abl tyrosine kinase of human leukemias is shown here to abrogate only the anchorage, not the growth factor, requirement. Bcr-Abl-transformed cells grow in soft agar but do not proliferate in serum-...

  2. Nuclear proliferation and safeguards. Summary

    This comprehensive analysis of the technological, economic, and political factors affecting the potential spread of nuclear weapons proved useful in the congressional debate which culminated in the Nuclear Non-Proliferation Act of 1978. The report was subsequently published commercially and has been a frequently cited reference in the literature on proliferation and nuclear power. Despite developments since 1977, the information in the OTA report is still useful to those wishing to obtain an indepth understanding of the issues. Included is an analysis of why a nation might want nuclear weapons development program and the various sources of nuclear material are discussed. The control of proliferation is considered as well as its relation to the nuclear industry

  3. Proliferation aspects of plutonium recycling

    Plutonium recycling offers benefits in an energy perspective of sustainable development, and, moreover it contributes to non-proliferation. Prior to recycling, reactor-grade plutonium from light-water reactors does not lend itself easily to the assembly of explosive nuclear devices; thereafter, practically not at all. Control systems for material security and non-proliferation should identify and adopt several categories of plutonium covering various isotopic mixtures associated with different fuel types, in order to better reflect the risks and to better focus their controls. The author proposes the adoption of three categories of plutonium. (author)

  4. Ophthalmic manifestations of methylmalonic aciduria accompanied with homocystinuria

    Qiu-Jing Huang

    2015-12-01

    Full Text Available Methylmalonicaciduia(MMAaccompanied with homocystinuria is a rare autosomal-recessive with congenital metabolic disorder of Vitamin B12. There are three subtypes, cblC, cblD, cblF, in which cblC is the most common one. The diagnostic tests are tandem mass spectrometry and gas chromatography-mass spectrometry. Tests for activity of enzyme in fibroblasts from skin, complementary assay and genetic analysis can be used to make the subtype clear. Early-onset patients, defined by onset of symptoms before the age of 1 year, may have severe ocular involvement, including visual loss, nystagmus, strabismus, retinopathy, maculopathy, optic atrophy, abnormal electroretinography. Late-onset patients, defined by onset of symptoms after the age of 4 year, rarely have ocular manifestations. The pathogenesis of the ophthalmic symptoms may be related to the high level of homocystine, oxidative stress and the abnormal development of nervous systems. The treatment for MMA accompanied with homocystinuria is mostly symptomatic based. Ophthalmic treatment is limited. Early supplement of methionine,GSH or other antioxidants may be helpful for retinopathy. There is no standard ophthalmological examination for those patients in China. It is critical to set up inter-departmental cooperation and early stage examination for the treatments and outcomes of the patients.

  5. Treatment of hepatocellular carcinoma accompanied by portal vein tumor thrombus

    Masami Minagawa; Masatoshi Makuuchi

    2006-01-01

    The prognosis of patients with hepatocellular carcinoma (HCC)accompanied by portal vein tumor thrombus (PVTT) is generally poor if left untreated: a median survival time of 2.7-4.0 mo has been reported. Furthermore, while transcatheter arterial chemoembolization (TACE) has been shown to be safe in selected patients, the median survival time with this treatment is still only 3.8-9.5 mo. Systemic single-agent chemotherapy for HCC with PVTT has failed to improve the prognosis, and the response rates have been less than 20%. While regional chemotherapy with low-dose cisplatin and 5-fluorouracil or interferon and 5-fluorouracil via hepatic arterial infusion has increased the response rate, the median survival time has not exceeded 12 (range 4.5-11.8) mo.Combined treatment consisting of radiation for PVTT and TACE for liver tumor has achieved a high response rate, but the median survival rates have still been only 3.8-10.7 mo. With hepatic resection as monotherapy,the 5-year survival rate and median survival time were reportedly 4%-28.5% and 6-14 mo. The most promising results were reported for combined treatments consisting of hepatectomy and TACE, chemotherapy, or internal radiation. The reported 5-year survival rates and median survival times were 42% and 31 mo for TACE followed by hepatectomy; 36.3% and 22.1 mo for hepatectomy followed by hepatic arterial infusion chemotherapy; and 56% for chemotherapy or internal radiation followed by hepatectomy.

  6. Thyroid Metastasis from Breast Carcinoma Accompanied by Papillary Thyroid Carcinoma

    Song-I Yang

    2014-07-01

    Full Text Available Metastasis to the thyroid gland is very rare. Recently, we experienced a case of thyroid metastasis from breast cancer accompanying a papillary thyroid. A 51-year-old female patient presented with a palpated lymph node on her left lateral neck. The patient had undergone a left modified radical mastectomy followed by chemotherapy and hormonal therapy 12 years prior. Ultrasonography of the neck revealed a malignant looking nodule at the left thyroid lobe, measuring 0.9 × 0.9 cm, and several cystic nodules at the right thyroid lobe. Ultrasonography of the neck additionally revealed a malignant looking lymph node at the right level VI. Fine-needle aspiration of the left thyroid lobe resulted in a diagnosis of papillary thyroid carcinoma and that of the right level VI in Hurthle cell lesion. The patient had a total thyroidectomy with selective dissection of the left neck node. Pathologic assessment of the specimen revealed metastatic carcinoma from the breast carcinoma and papillary thyroid carcinoma. Although the thyroid gland is highly vascularized, metastasis of malignant tumors to the thyroid is relatively rare and detection of metastasis shows a low frequency. So a careful evaluation of thyroid tumor should be considered in a patient with a history of other malignancy.

  7. Radio rebrightening of the GRB afterglow by the accompanying supernova

    Duran, Rodolfo Barniol

    2015-01-01

    The gamma-ray burst (GRB) jet powers the afterglow emission by shocking the surrounding medium, and radio afterglow can now be routinely observed to almost a year after the explosion. Long-duration GRBs are accompanied by supernovae (SNe) that typically contain much more energy than the GRB jet. Here we consider the fact that the SN blast wave will also produce its own afterglow, which will peak at much later time (since it is non-relativistic), when the SN blast wave transitions from a coasting phase to a decelerating Sedov-Taylor phase. We predict that this component will peak generally a few tens of years after the explosion and it will outshine the GRB powered afterglow well-before its peak emission. In the case of GRB 030329, where the external density is constrained by the $\\sim 10$-year coverage of the radio GRB afterglow, the radio emission is predicted to start rising over the next decade and to continue to increase for the following decades up to a level of $\\sim 0.5$ mJy. Detection of the SN-powere...

  8. Two cases of rectal cancer accompanied with radiation colitis

    This paper presents two cases of rectal cancer accompanied with radiation colitis. Case 1 was a 53-year-old woman, who had a history of undergoing radiation therapy for a uterine cervical cancer 11 years before. She was seen at the hospital because of constipation and pointed out a IIa-like lesion on the rectum by colonoscopy. Abdominoperineal resection was performed. The surgical specimen showed the IIa-like lesion on the rectum. Pathological findings revealed well-differentiated adenocarcinoma. Immunohistochemical staining of p53 showed positive cells in atrophic glands. Case 2 was a 62-year-old woman complaining of diarrhea. There was a previous history of receiving radiation therapy for a uterine cancer 20 years before. Colonoscopy showed a Borrmann type 2 cancer on the rectum. Abdominoperineal resection was performed. Histological findings revealed moderately differentiated adenocarcinoma invading to the propria muscle. The features of radiation colitis were observed around the cancer in the two cases which provided a clue to diagnose the lesions with radiation-induced cancer. (author)

  9. Change of local social economy accompanying location of power stations

    The method of environment assessment is urgently developed to make various development plans appropriate. It is desirable to grasp synthetically direct and indirect influences to society, since various development activities are deeply related to whole local societies. The Central Research Institute of Electric Power Industry has performed research to develop the method of synthesizing environment assessment, and this is the interim report on the development of the method for forecasting and evaluating the social influence accompanying the location of power stations. As the first approach, the development of the model to forecast the influence of location was started, using the econometric method. In this paper, the works of composing the pilot model, which was made to examine the possibility of model development, are summarized. The fundamental framework of model composition and the theoretical model for each power source and each locality were examined, and the works of making the proving models related to three points based on the theoretical model were carried out. As the result of this work of pilot model composition, the prospect of developing the econometric model for forecasting social environmental influence was obtained, and the works of developing the more versatile master model are advanced at present. (Kako, I.)

  10. Change of local social economy accompanying location of power stations

    Arai, Yasuo; Saito, Kannosuke (Central Research Inst. of Electric Power Industry, Tokyo (Japan))

    1982-05-01

    The method of environment assessment is urgently developed to make various development plans appropriate. It is desirable to grasp synthetically direct and indirect influences to society, since various development activities are deeply related to whole local societies. The Central Research Institute of Electric Power Industry has performed research to develop the method of synthesizing environment assessment, and this is the interim report on the development of the method for forecasting and evaluating the social influence accompanying the location of power stations. As the first approach, the development of the model to forecast the influence of location was started, using the econometric method. In this paper, the works of composing the pilot model, which was made to examine the possibility of model development, are summarized. The fundamental framework of model composition and the theoretical model for each power source and each locality were examined, and the works of making the proving models related to three points based on the theoretical model were carried out. As the result of this work of pilot model composition, the prospect of developing the econometric model for forecasting social environmental influence was obtained, and the works of developing the more versatile master model are advanced at present.

  11. Radio rebrightening of the GRB afterglow by the accompanying supernova

    Barniol Duran, R.; Giannios, D.

    2015-12-01

    The gamma-ray burst (GRB) jet powers the afterglow emission by shocking the surrounding medium, and radio afterglow can now be routinely observed to almost a year after the explosion. Long-duration GRBs are accompanied by supernovae (SNe) that typically contain much more energy than the GRB jet. Here we consider the fact that the SN blast wave will also produce its own afterglow (supernova remnant emission), which will peak at much later time (since it is non-relativistic), when the SN blast wave transitions from a coasting phase to a decelerating Sedov-Taylor phase. We predict that this component will peak generally a few tens of years after the explosion and it will outshine the GRB powered afterglow well-before its peak emission. In the case of GRB 030329, where the external density is constrained by the ˜10-year coverage of the radio GRB afterglow, the radio emission is predicted to start rising over the next decade and to continue to increase for the following decades up to a level of ˜ mJy. Detection of the SN-powered radio emission will greatly advance our knowledge of particle acceleration in ˜0.1c shocks.

  12. Exercise-induced promotion of hippocampal cell proliferation requires beta-endorphin.

    Koehl, M; Meerlo, P; Gonzales, D; Rontal, A; Turek, F W; Abrous, D N

    2008-07-01

    Adult hippocampal neurogenesis is influenced by a variety of stimuli, including exercise, but the mechanisms by which running affects neurogenesis are not yet fully understood. Because beta-endorphin, which is released in response to exercise, increases cell proliferation in vitro, we hypothesized that it could exert a similar effect in vivo and mediate the stimulatory effects of running on neurogenesis. We thus analyzed the effects of voluntary wheel-running on adult neurogenesis (proliferation, differentiation, survival/death) in wild-type and beta-endorphin-deficient mice. In wild-type mice, exercise promoted cell proliferation evaluated by sacrificing animals 24 h after the last 5-bromo-2'-deoxyuridine (BrdU) pulse and by using endogenous cell cycle markers (Ki67 and pH(3)). This was accompanied by an increased survival of 4-wk-old BrdU-labeled cells, leading to a net increase of neurogenesis. Beta-endorphin deficiency had no effect in sedentary mice, but it completely blocked the running-induced increase in cell proliferation; this blockade was accompanied by an increased survival of 4-wk-old cells and a decreased cell death. Altogether, adult neurogenesis was increased in response to exercise in knockout mice. We conclude that beta-endorphin released during running is a key factor for exercise-induced cell proliferation and that a homeostatic balance may regulate the final number of new neurons. PMID:18263701

  13. Nuclear non-Proliferation treaty

    The text of a speech is presented delivered by the State President of South Africa, Mr. F.W. de Klerk, to a joint session of Parliament on 24 March 1993, announcing developments relating to South Africa's nuclear capability and accession to the Treaty on the Non-Proliferation of Nuclear Weapons

  14. Nuclear Proliferation Technology Trends Analysis

    Zentner, Michael D.; Coles, Garill A.; Talbert, Robert J.

    2005-10-04

    A process is underway to develop mature, integrated methodologies to address nonproliferation issues. A variety of methodologies (both qualitative and quantitative) are being considered. All have one thing in common, a need for a consistent set of proliferation related data that can be used as a basis for application. One approach to providing a basis for predicting and evaluating future proliferation events is to understand past proliferation events, that is, the different paths that have actually been taken to acquire or attempt to acquire special nuclear material. In order to provide this information, this report describing previous material acquisition activities (obtained from open source material) has been prepared. This report describes how, based on an evaluation of historical trends in nuclear technology development, conclusions can be reached concerning: (1) The length of time it takes to acquire a technology; (2) The length of time it takes for production of special nuclear material to begin; and (3) The type of approaches taken for acquiring the technology. In addition to examining time constants, the report is intended to provide information that could be used to support the use of the different non-proliferation analysis methodologies. Accordingly, each section includes: (1) Technology description; (2) Technology origin; (3) Basic theory; (4) Important components/materials; (5) Technology development; (6) Technological difficulties involved in use; (7) Changes/improvements in technology; (8) Countries that have used/attempted to use the technology; (9) Technology Information; (10) Acquisition approaches; (11) Time constants for technology development; and (12) Required Concurrent Technologies.

  15. Developing the Sufficiency Perception Scale in the Area of Accompaniment with Piano for Candidate Music Teachers

    PİJİ, Duygu

    2013-01-01

    The music teacher’s accompanying the songs by piano in music education at schoolappears to be important regarding the development of melody, rhythm and harmony feelingsof the students. The music teacher candidates receive their knowledge and skill in the areaof piano accompaniment through the Accompaniment lesson within the Music TeachingLicense Program. In this research, with the purpose of defining the perception of sufficiencyregarding the accompaniment levels of music teacher candidates a...

  16. Fenestrations accompanied by intracranial aneurysms assessed with magnetic resonance angiography

    Zhen-Kui Sun

    2012-01-01

    Full Text Available Background and Purpose: The aim of this study was to evaluate the anatomical changes and investigate the prevalence in intracranial aneurysm with fenestrations using magnetic resonance angiography (MRA. Materials and Methods: Between June 2008 and October 2010, 4652 patients (aged 23-73 years with suspected intracranial aneurysm or other cerebrovascular diseases underwent MRA examination. MRA was performed using a three-dimensional time-of-flight technique (3D-TOF with volume rendering (VR and maximum intensity projection reconstruction methods. The presence and location of fenestrations and aneurysms was reviewed. When fenestrations were present in combination with aneurysms, we noted the relationship of the locations. The classification of fenestration accompanied by intracranial aneurysm was divided into three types according to the anatomical relationship as follows: Type I, aneurysm adjacent to but not on a fenestration; Type II, aneurysm located on the fenestration; type III, aneurysm located at a position remote from a fenestration. Results: Among the 4652 patients examined, 409 patients were defined with 412 intracranial aneurysms, and the prevalence of aneurysms was 8.8%. One hundred and forty-one patients were identified with fenestrations; 24 of these patients were confirmed with intracranial aneurysms. Seven cases were classified as type I, three as type II and 14 as type III. The prevalence of intracranial aneurysm with fenestrations was 17.0%, with significant statistical difference compared with aneurysms unaccompanied with fenestrations (P=0.0064. Conclusion: The anatomical relationship between fenestrations and intracranial aneurysms was visualized by MRA with VR, which displayed pathologies with sufficient clarity to enable diagnosis. Furthermore, the results of this study suggest that physicians should be alerted to the occurrence of intracranial aneurysm following the detection of fenestrations by MRA.

  17. Hemiplegic peripheral neuropathy accompanied with multiple cranial nerve palsy

    Hirohisa Okuma

    2012-03-01

    Full Text Available A 32-year-old man experienced double vision around January, 2010, followed by weakness of his left upper and lower extremities. Articulation disorders and loss of hearing in his left ear developed, and he was admitted to our hospital on February 14, 2010. Physical examination was normal, and neurological examination showed clear consciousness with no impairment of cognitive function, but with articulation disorders. Olfactory sensation was reduced. Left ptosis and left gaze palsy, complete left facial palsy, perceptive deafness of the left ear, and muscle weakness of the left trapezius muscle were observed. Paresis in the left upper and lower extremities was graded 4/5 through manual muscle testing. Sensory system evaluation revealed complete left-side palsy, including the face. Deep tendon reflexes were slightly diminished equally on both sides; no pathologic reflex was seen. No abnormality of the brain parenchyma, cerebral nerves or cervicothoracolumbar region was found on brain magnetic resonance imaging. On electroencephalogram, alpha waves in the main frequency band of 8 to 9 Hz were recorded, indicating normal findings. Brain single photon emission computed tomography (SPECT scan showed reduced blood flow in the right inner frontal lobe and both occipital lobes. Nerve biopsy (left sural nerve showed reduction of nerve density by 30%, with demyelination. The patient also showed manifestations of multiple cranial nerve disorder, i.e., of the trigeminal nerve, glossopharyngeal nerve, vagus nerve, and hypoglossal nerve. Whole-body examination was negative. Finally, based on ischemic brain SPECT images, spinal fluid findings and nerve biopsy results, peripheral neuropathy accompanied with multiple cranial nerve palsy was diagnosed.

  18. 5 CFR 581.203 - Information minimally required to accompany legal process.

    2010-01-01

    ... accompany legal process. 581.203 Section 581.203 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT... Process § 581.203 Information minimally required to accompany legal process. (a) Sufficient identifying information must accompany the legal process in order to enable processing by the governmental entity...

  19. NFAT5 promotes proliferation and migration of lung adenocarcinoma cells in part through regulating AQP5 expression

    Guo, Kai, E-mail: gk161@163.com [Department of Respiration, Tangdu Hospital, Fourth Military Medical University, Xi' an 710038 (China); Department of Respiration, 161th Hospital, PLA, Wuhan 430015 (China); Jin, Faguang, E-mail: jinfag@fmmu.edu.cn [Department of Respiration, Tangdu Hospital, Fourth Military Medical University, Xi' an 710038 (China)

    2015-09-25

    The osmoregulated transcription factor nuclear factor of activated T-cells 5(NFAT5), has been found to play important roles in the development of many kinds of human cancers, including breast cancer, colon carcinoma, renal cell carcinoma and melanoma. The aim of the present study was to determine whether NFAT5 is involved in the proliferation and migration of lung adenocarcinoma cells. We found that NFAT5 was upregulated in lung adenocarcinoma cells and knockdown of NFAT5 decreased proliferation and migration of the cells, accompanied by a significant reduction in the expression of AQP5. AQP5 was upregulated in lung adenocarcinoma cells and knockdown of AQP5 also inhibited proliferation and migration of the cells as knockdown of NFAT5 did. Moreover, overexpression of NFAT5 promoted proliferation and migration of lung adenocarcinoma cells, accompanied by a significant increase in the expression of AQP5. These results indicate that NFAT5 plays important roles in proliferation and migration of human lung adenocarcinoma cells through regulating AQP5 expression, providing a new therapeutic option for lung adenocarcinoma therapy. - Highlights: • NFAT5 expression is higher in lung adenocarcinoma cells compared with normal cells. • NFAT5 knockdown decreases proliferation and migration of lung adenocarcinoma cells. • Knockdown of NFAT5 reduces AQP5 expression in human lung adenocarcinoma cells. • Overexpression of NFAT5 promotes proliferation and migration of lung adenocarcinoma cells. • Overexpression of NFAT5 increases AQP5 expression in human lung adenocarcinoma cells.

  20. NFAT5 promotes proliferation and migration of lung adenocarcinoma cells in part through regulating AQP5 expression

    The osmoregulated transcription factor nuclear factor of activated T-cells 5(NFAT5), has been found to play important roles in the development of many kinds of human cancers, including breast cancer, colon carcinoma, renal cell carcinoma and melanoma. The aim of the present study was to determine whether NFAT5 is involved in the proliferation and migration of lung adenocarcinoma cells. We found that NFAT5 was upregulated in lung adenocarcinoma cells and knockdown of NFAT5 decreased proliferation and migration of the cells, accompanied by a significant reduction in the expression of AQP5. AQP5 was upregulated in lung adenocarcinoma cells and knockdown of AQP5 also inhibited proliferation and migration of the cells as knockdown of NFAT5 did. Moreover, overexpression of NFAT5 promoted proliferation and migration of lung adenocarcinoma cells, accompanied by a significant increase in the expression of AQP5. These results indicate that NFAT5 plays important roles in proliferation and migration of human lung adenocarcinoma cells through regulating AQP5 expression, providing a new therapeutic option for lung adenocarcinoma therapy. - Highlights: • NFAT5 expression is higher in lung adenocarcinoma cells compared with normal cells. • NFAT5 knockdown decreases proliferation and migration of lung adenocarcinoma cells. • Knockdown of NFAT5 reduces AQP5 expression in human lung adenocarcinoma cells. • Overexpression of NFAT5 promotes proliferation and migration of lung adenocarcinoma cells. • Overexpression of NFAT5 increases AQP5 expression in human lung adenocarcinoma cells

  1. Dehydroepiandroesteron Accompanied Retinoic Acid Enhances Differentiation of P19 Embryonal Stem Cells into Neural Cells

    Hossein Azizi

    2009-01-01

    Full Text Available Objective: Dehydroepiandroesteron (DHEA is a neurosteroid with potential effect on neurogenesis,neuronal survival and proliferation of neural progenitor cells. However there is nodirect evidence for its biological effect during the differentiation of stem cell-derived neurons.The p19 line of embryonal carcinoma cells develops into neurons, astroglia and fibroblastsafter exposure to retinoic acid (RA. This study was initiated to assess the effect of DHEA onneural cells derived from p19 embryonal carcinoma stem cells.Materials and Methods: P19 cells were suspended in dulbecco’s modified eagle’s medium(DMED containing fetal bovine serum (FBS in bacterial-grade petri dishes in the presenceof RA, DHEA and RA+DHEA for 6 days. Then cells were trypsinized for dispersion and replacedin poly L- lysine (10μg/ml coated tissue culture dishes without RA and DHEA for 4days. The expression of neural markers Map-2, Tau, beta-tubulin III- clone Juj (Tuj1, astrocytemarker GFAP and the percent of neurotransmitters tyrosin hydroxylase, glutamate, serotoninand actyl cholin transferase were evaluated by flowcytometry, immunocytochemistryand RT-PCR analysis.Results: Flowcytometry analysis showed that about 63 ± 3% of the cells express neuronalmarker Tuj1 and about 5 ± 1% of the cells express tyrosine hydroxylase neurotransmittersin RA treated groups. However when RA and DHEA were added to the culture medium, Tuj1expression increased to about 74 ± 1% and tyrosine hydroxylase expression increased to23 ± 2%.Conclusion: Results showed that DHEA accompanied RA increased the number of Tuj1 anddopaminergic neurons that were derived from p19 embryonal carcinoma stem cells.

  2. Research on the dry intrusion accompanying the low vortex precipitation

    2007-01-01

    By employing the 6.7μm satellite vapor cloud images and NCEP/NCAR 1°×1° reanalysis datasets, the characteristics and mechanism of the dry intrusion, as well as its impacts on the low vortex precipitation at the Meiyu front are explored in this paper. It is found that the formation,development and maintenance of the low vortex precipitation at the Meiyu front are closely related to the evolution of the dry intrusion. The dry intrusion is characterized by high potential vorticity (PV), low humidity and cold air. The dry intrusion exhibits as an obvious dark zone on vapor cloud images, an area in which atmospheric relative humidity is lower than 60%. However, the features of the dry intrusion on the vapor images are clearer than that of the humidity field, for the former is the digital vapor cloud images with high temporal and spatial resolution, and it can be used to explore the finer characteristics of the development, evolution and supplement of the intrusion during the development of the low vortex. The dry intrusion impacts accompanying the low vortex precipitation at the Meiyu front come from all levels of the troposphere, with the strongest intrusion located at the upper troposphere. The dry and cold air intrudes the vicinity of the low vortex from the upper isentropic surface to the lower one, slanting east-ward from lower to higher level. The low vortex precipitation region is usually situated in front of the dry intrusion where the relative humidity gradient is higher. The research also reveals that the mechanism of the dry intrusion is that the high potential vorticity descends from the upper troposphere to the lower level, therefore, the dry intrusion can be used as an important index of the high PV forcing. To the west of the low vortex precipitation, the upper level northerlies descend across the isentropic surface, then the dry cold advection can trigger the instable development in the midlow troposphere. The dry intru-sion enhances the low vortex

  3. Proliferation Vulnerability Red Team report

    Hinton, J.P.; Barnard, R.W.; Bennett, D.E. [and others

    1996-10-01

    This report is the product of a four-month independent technical assessment of potential proliferation vulnerabilities associated with the plutonium disposition alternatives currently under review by DOE/MD. The scope of this MD-chartered/Sandia-led study was limited to technical considerations that could reduce proliferation resistance during various stages of the disposition processes below the Stored Weapon/Spent Fuel standards. Both overt and covert threats from host nation and unauthorized parties were considered. The results of this study will be integrated with complementary work by others into an overall Nonproliferation and Arms Control Assessment in support of a Secretarial Record of Decision later this year for disposition of surplus U.S. weapons plutonium.

  4. An approximate approach of heat transfer accompanied by phase transition

    Low temperature heat (solar energy, geothermal energy, industrial and domestic waste heat) is widely available for many applications. Energy storage of that heat is now of great importance because the key to the effective and widespread use of low temperature heat is its adaptation to the energy requirements. From this point of view, storage tanks based on the phase change principal are one interesting alternative. Phase change material (PCM) is particularly attractive due to its ability to provide a high-energy storage density and its characteristics to store heat at a constant temperature corresponding to the phase transition temperature of the heat storage material. That is why the problem of the heat transfer accompanied by phase transition is of mine interest to the practice. Heat storage system with the PCM and shell-and-tube type is analysed by Lacroix [6]. Several authors, using mathematical models of different complexity, studied this type of. latent heat storage unit. Heat-of-fusion storage materials for law temperature storage in the temperature range 0-120oC are reviewed by Abhat [5] and Reiter and Rota [4]. Hamdan and Elweer [3] have investigated a melting process of a solid phase. In the present study, the phase change problem of the PCM is analysed in another type of heat storage system. We use closed tubes (capsules) filled with PCM and the heat transfer fluid (HTF) flowing in the shell space of the heat exchanger (Fig.1). The heat process is analysed in terms of both radial and axial direction and is linked to the convective heat transfer from the HTF. As it is known [2,3] the transient heat transfer in PCM can be described by the well-known heat conduction equation applied for various phase conditions and the energy balance on the interface given by the Stephan's equation. The later determines the rate of moving of the interface and in this manner the space areas where the heat conduction equations have corresponding coefficients. The application

  5. Inverse relationship between adult hippocampal cell proliferation and synaptic rewiring in the dentate gyrus.

    Butz, Markus; Teuchert-Noodt, Gertraud; Grafen, Keren; van Ooyen, Arjen

    2008-01-01

    Adult neurogenesis is a key feature of the hippocampal dentate gyrus (DG). Neurogenesis is accompanied by synaptogenesis as new cells become integrated into the circuitry of the hippocampus. However, little is known to what extent the embedding of new neurons rewires the pre-existing network. Here we investigate synaptic rewiring in the DG of gerbils (Meriones unguiculatus) under different rates of adult cell proliferation caused by different rearing conditions as well as juvenile methamphetamine treatment. Surprisingly, we found that an increased cell proliferation reduced the amount of synaptic rewiring. To help explain this unexpected finding, we developed a novel model of dentate network formation incorporating neurogenesis and activity-dependent synapse formation and remodelling. In the model, we show that homeostasis of neuronal activity can account for the inverse relationship between cell proliferation and synaptic rewiring. PMID:18481284

  6. Nanoparticles for cells proliferation enhancement

    The potential of semiconductor nanoparticles as stimulator for avian mesenchyme stem cells proliferation enhancement is demonstrated. The effect is related to nanoparticles polarization due to external ultrasound field resulting in local electrical stimulation. Our preliminary results demonstrates that the number of cells have been increased by 23 % ±2%) in cell cultures under the action of external ultrasound stimulation. Morphological analysis and viability shows no differences between the control group and the group studied. These results suggest the possibility for tissue regeneration enhancement by remote stimulation of implanted semiconductor nanoparticles. (authors)

  7. Fissile material disposition and proliferation risk

    The proliferation risk of a facility is dependent on the material attractiveness, level of safeguards, and physical protection applied to the material in conjunction with an assessment of the impact of the socioeconomic circumstances and threat environment. Proliferation risk is a complementary extension of proliferation resistance. The authors believe a better determination of nuclear material proliferation can be achieved by establishing the proliferation risk for facilities that contain nuclear material. Developing a method that incorporates the socioeconomic circumstances and threat environment inherent to each country enables a global proliferation assessment. In order to effectively reduce the nuclear danger, a broadly based set of criteria is needed that provides the capability to relatively assess a wide range of disposition options/facilities in different countries and still ensure a global decrease in proliferation risk for plutonium

  8. Non-proliferation under revision

    At the 3rd NPT Revision Conference held in Geneva between August 27 and September 21, 1985, the member states to the Treaty reconfirmed their conviction that the agreement had become an essential part in the efforts to secure peace in the world and that is fundamental objectives continued to serve this purpose. Since the ratification in 1968 and the entering into force in 1970 of the Non-proliferation Treaty, the number of member states had risen continually to, at present, 130. In 1975, 92 states in 1980, 114 states had signed the agreement. The three nuclear weapon states, USA, UK, and USSR, are depositary states. France acts as if she had acceded to the Treaty, and the People's Republic of China as the 5th nuclear weapon state seems to take steps in the same direction by voluntarily opening nuclear installations to checks by IAEA inspectors. Incidentally, an effective framework of non-proliferation in the South American region has been created in the Treaty of Tlatelolco. 98% of all nuclear installations in the non-nuclear weapon countries are covered by IAEA safeguards. (orig.)

  9. Negative regulators of cell proliferation

    Johnson, T. C.; Spooner, B. S. (Principal Investigator)

    1994-01-01

    Cell proliferation is governed by the influence of both mitogens and inhibitors. Although cell contact has long been thought to play a fundamental role in cell cycling regulation, and negative regulators have long been suspected to exist, their isolation and purification has been complicated by a variety of technical difficulties. Nevertheless, over recent years an ever-expanding list of putative negative regulators have emerged. In many cases, their biological inhibitory activities are consistent with density-dependent growth inhibition. Most likely their interactions with mitogenic agents, at an intracellular level, are responsible for either mitotic arrest or continued cell cycling. A review of naturally occurring cell growth inhibitors is presented with an emphasis on those factors shown to be residents of the cell surface membrane. Particular attention is focused on a cell surface sialoglycopeptide, isolated from intact bovine cerebral cortex cells, which has been shown to inhibit the proliferation of an unusually wide range of target cells. The glycopeptide arrest cells obtained from diverse species, both fibroblasts and epithelial cells, and a broad variety of transformed cells. Signal transduction events and a limited spectrum of cells that are refractory to the sialoglycopeptide have provided insight into the molecular events mediated by this cell surface inhibitor.

  10. Gas Centrifuges and Nuclear Proliferation

    Albright, David

    2004-09-15

    Gas centrifuges have been an ideal enrichment method for a wide variety of countries. Many countries have built gas centrifuges to make enriched uranium for peaceful nuclear purposes. Other countries have secretly sought centrifuges to make highly enriched uranium for nuclear weapons. In more recent times, several countries have secretly sought or built gas centrifuges in regions of tension. The main countries that have been of interest in the last two decades have been Pakistan, Iraq, Iran, and North Korea. Currently, most attention is focused on Iran, Pakistan, and North Korea. These states did not have the indigenous abilities to make gas centrifuges, focusing instead on illicit and questionable foreign procurement. The presentation covered the following main sections: Spread of centrifuges through illicit procurement; Role of export controls in stopping proliferation; Increasing the transparency of gas centrifuge programs in non-nuclear weapon states; and, Verified dismantlement of gas centrifuge programs. Gas centrifuges are important providers of low enriched uranium for civil nuclear power reactors. They also pose special nuclear proliferation risks. We all have special responsibilities to prevent the spread of gas centrifuges into regions of tension and to mitigate the consequences of their spread into the Middle East, South Asia, and North Asia.

  11. Co-culture with Sertoli cells promotes proliferation and migration of umbilical cord mesenchymal stem cells

    Highlights: ► Co-culture of Sertoli cells (SCs) with human umbilical cord mesenchymal stem cells (UCMSCs). ► Presence of SCs dramatically increased proliferation and migration of UCMSCs. ► Presence of SCs stimulated expression of Mdm2, Akt, CDC2, Cyclin D, CXCR4, MAPKs. -- Abstract: Human umbilical cord mesenchymal stem cells (hUCMSCs) have been recently used in transplant therapy. The proliferation and migration of MSCs are the determinants of the efficiency of MSC transplant therapy. Sertoli cells are a kind of “nurse” cells that support the development of sperm cells. Recent studies show that Sertoli cells promote proliferation of endothelial cells and neural stem cells in co-culture. We hypothesized that co-culture of UCMSCs with Sertoli cells may also promote proliferation and migration of UCMSCs. To examine this hypothesis, we isolated UCMSCs from human cords and Sertoli cells from mouse testes, and co-cultured them using a Transwell system. We found that UCMSCs exhibited strong proliferation ability and potential to differentiate to other cell lineages such as osteocytes and adipocytes. The presence of Sertoli cells in co-culture significantly enhanced the proliferation and migration potential of UCMSCs (P < 0.01). Moreover, these phenotypic changes were accompanied with upregulation of multiple genes involved in cell proliferation and migration including phospho-Akt, Mdm2, phospho-CDC2, Cyclin D1, Cyclin D3 as well as CXCR4, phospho-p44 MAPK and phospho-p38 MAPK. These findings indicate that Sertoli cells boost UCMSC proliferation and migration potential.

  12. Co-culture with Sertoli cells promotes proliferation and migration of umbilical cord mesenchymal stem cells

    Zhang, Fenxi, E-mail: fxzhang0824@gmail.com [Department of Anatomy, Sanquan College, Xinxiang Medical University, Henan 453003, People' s Republic of China (China); Hong, Yan; Liang, Wenmei [Department of Histology and Embryology, Guiyang Medical University, Guizhou 550004, People' s Republic of China (China); Ren, Tongming [Department of Anatomy, Sanquan College, Xinxiang Medical University, Henan 453003, People' s Republic of China (China); Jing, Suhua [ICU Center, The Third Hospital of Xinxiang Medical University, Henan 453003, People' s Republic of China (China); Lin, Juntang [Stem Cell Center, Xinxiang Medical University, Henan 453003, People' s Republic of China (China)

    2012-10-12

    Highlights: Black-Right-Pointing-Pointer Co-culture of Sertoli cells (SCs) with human umbilical cord mesenchymal stem cells (UCMSCs). Black-Right-Pointing-Pointer Presence of SCs dramatically increased proliferation and migration of UCMSCs. Black-Right-Pointing-Pointer Presence of SCs stimulated expression of Mdm2, Akt, CDC2, Cyclin D, CXCR4, MAPKs. -- Abstract: Human umbilical cord mesenchymal stem cells (hUCMSCs) have been recently used in transplant therapy. The proliferation and migration of MSCs are the determinants of the efficiency of MSC transplant therapy. Sertoli cells are a kind of 'nurse' cells that support the development of sperm cells. Recent studies show that Sertoli cells promote proliferation of endothelial cells and neural stem cells in co-culture. We hypothesized that co-culture of UCMSCs with Sertoli cells may also promote proliferation and migration of UCMSCs. To examine this hypothesis, we isolated UCMSCs from human cords and Sertoli cells from mouse testes, and co-cultured them using a Transwell system. We found that UCMSCs exhibited strong proliferation ability and potential to differentiate to other cell lineages such as osteocytes and adipocytes. The presence of Sertoli cells in co-culture significantly enhanced the proliferation and migration potential of UCMSCs (P < 0.01). Moreover, these phenotypic changes were accompanied with upregulation of multiple genes involved in cell proliferation and migration including phospho-Akt, Mdm2, phospho-CDC2, Cyclin D1, Cyclin D3 as well as CXCR4, phospho-p44 MAPK and phospho-p38 MAPK. These findings indicate that Sertoli cells boost UCMSC proliferation and migration potential.

  13. Study on proliferation time and response time for proliferation resistance evaluation

    'Proliferation time' is one of the proliferation resistance measures adopted by the Generation IV Nuclear Energy Systems International Forum (GIF) Proliferation Resistance and Physical Protection (PR and PP) evaluation methodology. A longer proliferation time would provide the international society with more time to intervene politically in order to dissuade the State from completing its nuclear weapons program. A longer proliferation time would therefore contribute to the enhancement of proliferation resistance of a given nuclear energy system. Two methods are considered for judging whether the proliferation time is long enough: 1) comparison of the proliferation times between a reference nuclear energy system and the subject system, and 2) comparison between the proliferation time and the response time, which can be defined as the time available to the international society to make a political intervention. This paper focuses on the latter method and examines how the response time can be estimated by reviewing prior incidents. (author)

  14. Cell proliferation in vitro modulates fibroblast collagenase activity

    Collagenase enzyme activity is regulated by numerous control mechanisms which prevent excessive release and activation of this protease. A primary mechanism for regulating enzyme extracellular activity may be linked to cell division, therefore they have examined the release of collagenase by fibroblasts in vitro in response to cellular proliferation. Studies were performed using fibroblasts derived from adult rat dermis maintained in DMEM containing 10% newborn calf serum, 25 mM tricine buffer, and antibiotics. Cells between subculture 10 and 19 were used with enzyme activity determined with a 14C-labelled soluble Type I collagen substrate with and without trypsin activation. Fibroblasts, trypsinized and plated at low density secreted 8.5 fold more enzyme than those cells at confluence (975 vs. 115 dpm/μg DNA). This diminution occurred gradually as the cells went from logrithmic growth towards confluence. Confluent fibroblast monolayers were scraped in a grid arrangement, stimulating the remaining cells to divide, without exposure to trypsin. Within 24-48 hr postscraping enzyme levels had increased 260-400%, accompanied by enhanced incorporation of 3H-thymidine and 3H-uridine into cell macromolecules. The burst of enzyme release began to subside 12 hr later. These results support a close relationship between fibroblast proliferation and collagenase secretion

  15. Selective cytotoxicity of benzyl isothiocyanate in the proliferating fibroblastoid cells.

    Miyoshi, Noriyuki; Uchida, Koji; Osawa, Toshihiko; Nakamura, Yoshimasa

    2007-02-01

    In the present study, experiments using presynchronization culture cells demonstrated that benzyl ITC (BITC), previously isolated from a tropical papaya fruit extract, induced the cytotoxic effect preferentially in the proliferating human colon CCD-18Co cells to the quiescent ones. Quiescent CCD-18Co cells were virtually unaffected by BITC and marginal cytotoxicity was observed at 15 microM. We observed that BITC dramatically induced the p53 phosphorylation and stabilization only in the quiescent (G(0)/G(1) phase-arrested) cells, but not significantly in the proliferating human colon CCD-18Co cells when compared with quiescent ones. We also observed ataxia telangiectasia-mutated (ATM) phosphorylation in the quiescent cells. The BITC-induced p53 phosphorylation was counteracted by caffeine treatment, implying the involvement of an ATM/ataxia telangiectasia and Rad3-related kinase signaling pathway. Moreover, downregulation of p53 by a siRNA resulted in the enhancement of susceptibility to undergo apoptosis by BITC. We also showed here that depletion of p53 abrogated G(0)/G(1) arrest accompanied by the declined expression of p21(waf1/cip1) and p27(kip1) in CCD-18Co cells. In conclusion, we identified p53 as a potential negative regulator of the apoptosis induction by BITC in the normal colon CCD-18Co cells through the inhibition of cell-cycle progression at the G(0)/G(1) phase. PMID:17096346

  16. RNA interference targeting raptor inhibits proliferation of gastric cancer cells

    Wu, William Ka Kei; Lee, Chung Wa [Institute of Digestive Diseases, LKS Institute of Health Sciences and Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Shatin, NT, Hong Kong (China); Cho, Chi Hin [Institute of Digestive Diseases, LKS Institute of Health Sciences and Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Shatin, NT, Hong Kong (China); School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong (China); Chan, Francis Ka Leung [Institute of Digestive Diseases, LKS Institute of Health Sciences and Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Shatin, NT, Hong Kong (China); Yu, Jun, E-mail: junyu@cuhk.edu.hk [Institute of Digestive Diseases, LKS Institute of Health Sciences and Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Shatin, NT, Hong Kong (China); Sung, Joseph Jao Yiu, E-mail: joesung@cuhk.edu.hk [Institute of Digestive Diseases, LKS Institute of Health Sciences and Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Shatin, NT, Hong Kong (China)

    2011-06-10

    Mammalian target of rapamycin complex 1 (mTORC1) is dysregulated in gastric cancer. The biologic function of mTORC1 in gastric carcinogenesis is unclear. Here, we demonstrate that disruption of mTORC1 function by RNA interference-mediated downregulation of raptor substantially inhibited gastric cancer cell proliferation through induction of G{sub 0}/G{sub 1}-phase cell cycle arrest. The anti-proliferative effect was accompanied by concomitant downregulation of activator protein-1 and upregulation of Smad2/3 transcriptional activities. In addition, the expression of cyclin D{sub 3} and p21{sup Waf1}, which stabilizes cyclin D/cdk4 complex for G{sub 1}-S transition, was reduced by raptor knockdown. In conclusion, disruption of mTORC1 inhibits gastric cancer cell proliferation through multiple pathways. This discovery may have an implication in the application of mTORC1-directed therapy for the treatment of gastric cancer.

  17. The Recall of Verbal Material Accompanying Semantically Well-Integrated and Semantically Poorly-Integrated Sentences.

    Rosenberg, Sheldon

    This study was designed to test the hypothesis that the recall of verbal material (critical material) accompanying semantically well integrated (SWI) sentences will be superior to the recall of verbal material accompanying semantically poorly integrated (SPI) sentences. This hypothesis was based upon the conclusion derived from previous research…

  18. 7 CFR 319.37-12 - Prohibited articles accompanying restricted articles.

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Prohibited articles accompanying restricted articles... Stock, Plants, Roots, Bulbs, Seeds, and Other Plant Products 1,2 § 319.37-12 Prohibited articles accompanying restricted articles. A restricted article for importation into the United States shall not...

  19. 5 CFR 582.203 - Information minimally required to accompany legal process.

    2010-01-01

    ... accompany legal process. 582.203 Section 582.203 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS COMMERCIAL GARNISHMENT OF FEDERAL EMPLOYEES' PAY Service of Legal Process § 582.203 Information minimally required to accompany legal process. (a) Sufficient...

  20. 22 CFR 40.102 - Guardian required to accompany excluded alien.

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Guardian required to accompany excluded alien. 40.102 Section 40.102 Foreign Relations DEPARTMENT OF STATE VISAS REGULATIONS PERTAINING TO BOTH... Guardian required to accompany excluded alien. INA 212(a)(9)(B) is not applicable at the time of...

  1. Artists-accompanied urban regeneration: Insights and lessons from Utrecht and Rotterdam

    Zebracki, M.M.; Smulders, L.

    2012-01-01

    Urban regeneration in Dutch cities is increasingly accompanied by the cultural sector in general and by artists in particular. This paper presents comparative case studies on current artists-accompanied urban regeneration initiated by housing corporations in Utrecht and Rotterdam. On the basis of di

  2. 39 CFR 320.7 - Suspension for advertisements accompanying parcels or periodicals.

    2010-07-01

    ... 39 Postal Service 1 2010-07-01 2010-07-01 false Suspension for advertisements accompanying parcels... advertisements accompanying parcels or periodicals. (a) The operation of 39 U.S.C. 601(a) (1) through (6) and § 310.2(b) (1) through (6) of this chapter is suspended on all post routes for advertisements...

  3. The Effects of Harmonic Accompaniment on the Tonal Improvisations of Students in First through Sixth Grade

    Guilbault, Denise Marie

    2009-01-01

    The purpose of this research was to examine the effects of harmonic accompaniment on the tonal improvisations of elementary school students. Specifically, this study was designed to (a) determine if the addition of a root melody accompaniment to song instruction affects the implied harmonic changes and harmonic rhythm in the tonal improvisations…

  4. Proliferation resistance fuel cycle technology

    The issues of dual use in nuclear technology are analysed for nuclear fuel cycle with special focus on uranium enrichment and spent fuel reprocessing which are considered as the most sensitive components in terms of vulnerability to diversion. Technical alternatives to mitigrate the vulnerability, as has been analysed in depth during the NASAP and INFCE era in the late seventies, are reviewed to characterize the DUPIC fuel cycle alternative. On the other hand, the new realities in nuclear energy including the disposition of weapon materials as a legacy of cold war are recast in an angle of nuclear proliferation resistance and safeguards with a discussion on the concept of spent fuel standard concept and its compliance with the DUPIC fuel cycle technology. (author)

  5. Proliferation resistance fuel cycle technology

    Lee, J. S.; Ko, W. I

    1999-02-01

    The issues of dual use in nuclear technology are analysed for nuclear fuel cycle with special focus on uranium enrichment and spent fuel reprocessing which are considered as the most sensitive components in terms of vulnerability to diversion. Technical alternatives to mitigrate the vulnerability, as has been analysed in depth during the NASAP and INFCE era in the late seventies, are reviewed to characterize the DUPIC fuel cycle alternative. On the other hand, the new realities in nuclear energy including the disposition of weapon materials as a legacy of cold war are recast in an angle of nuclear proliferation resistance and safeguards with a discussion on the concept of spent fuel standard concept and its compliance with the DUPIC fuel cycle technology. (author)

  6. Romania non-proliferation policy

    Full text: Non-proliferation concept in Romania is based on the Treaty on the Non-proliferation of Nuclear Weapons, which was ratified in 1970. According to the Article III of the Treaty, Romania ratified in 1972, the Agreement between Romania and IAEA for the application of Safeguards in connection with the Treaty on the Non-proliferation of Nuclear Weapons. In 2000 Romania ratified the Additional Protocol to contribute through increased transparency, to confidence that no undeclared nuclear activities are concealed within the declared programme or make use of elements of that programme. Under the Additional Protocol Romania understands to increase the transparency of its nuclear activities lengthways fuel cycle. Romania has a strong legal framework to control nuclear material and nuclear activities. The Law 111/1996, republished is the Law on the safe deployment of nuclear activities. CNCAN issued National Regulations for Safeguards and Physical Protection. Prospecting for uranium in Romania was initiated in 1950. Between 1962 and 1978 all the uranium ore production was stockpiled at the mine sites. In 1978 the Feldioara Powder Plant was commissioned, since then both ore stockpiles and ore exploited have been processed to uranium chemical concentrates. The Powder Plant Feldioara was conceived and built following the necessity of milling and processing the uranium ore to UO2, in concordance with the national nuclear programme in order to produce electric energy from nuclear fuel. The Nuclear Fuel Plant has capability to manufacture CANDU-6 nuclear fuel. Nuclear Fuel Plant consists of two Production areas, the Quality Assurance and Engineering Departments. There are two Production Departments: Pelleting area including granulation, pressing, sintering, pellet grinding, uranium recycling and Assembling area including components fabrication, beryllium coating, brazing, graphite coating, fuel element and bundle assembly welding. Romania's Strategy for Energy Sector is

  7. Nuclear energy and nuclear weapons proliferation

    A summary of the report dispatched in the middle of 1978 by the Atlantic Council of United States, organized by North American citizens, is presented. The report considers the relation between the production of nucleoelectric energy and the capacity of proliferation of nuclear weapons. The factors which affect the grade of proliferation risk represented by the use of nuclear energy in the world comparing this risk with the proliferation risks independently of nuclear energy, are examined. (M.C.K.)

  8. Proliferation resistance: issues, initiatives and evaluation

    Pilat, Joseph F [Los Alamos National Laboratory

    2009-01-01

    The vision of a nuclear renaissance has highlighted the issue of proliferation resistance. The prospects for a dramatic growth in nuclear power may depend on the effectiveness of, and the resources devoted to, plans to develop and implement technologies and approaches that strengthen proliferation resistance. The GenIV International Forum (GIF) and others have devoted attention and resources to proliferation resistance. However, the hope of finding a way to make the peaceful uses of nuclear energy resistant to proliferation has reappeared again and again in the history of nuclear power with little practical consequence. The concept of proliferation resistance has usually focused on intrinsic (technological) as opposed to extrinsic (institutional) factors. However, if there are benefits that may yet be realized from reactors and other facilities designed to minimize proliferation risks, it is their coupling with effective safeguards and other nonproliferation measures that likely will be critical. Proliferation resistance has also traditionally been applied only to state threats. Although there are no technologies that can wholly eliminate the risk of proliferation by a determined state, technology can play a limited role in reducing state threats and perhaps in eliminating many non-state threats. These and other issues are not academic. They affect efforts to evaluate proliferation resistance, including the methodology developed by GIF's Proliferation Resistance and Physical Protection (PR&PP) Working Group as well as the proliferation resistance initiatives that are being pursued or may be developed in the future. This paper will offer a new framework for thinking about proliferation resistance issues, including the ways the output of the methodology could be developed to inform the decisions that states, the International Atomic Energy (IAEA) and others will have to make in order to fully realize the promise of a nuclear renaissance.

  9. Modeling Nuclear Proliferation : Expanding Input Variable Sets

    Quantitative tools have been developed to analyze and nuclear proliferation events. However, the results from the current models show weaknesses in the model. This work is an attempt to improve upon existing models by adding new variables based on the understanding of nuclear proliferation scenarios. In this study, two more variables were added to existing dataset to enhance the nuclear proliferation prediction. The results showed enhancement of modeling capability for some countries, but some limitations still exist. Future work will include by adding new variables and improving database to further enhance proliferation modeling capability

  10. Strengthening the non proliferation regime: French views

    3 main issues can be identified in the French policy concerning the backing of non proliferation: 1) responding resolutely to proliferation crises, 2) reinforcing substantive efforts to prevent and impede proliferation, and 3) strengthening the non-proliferation regime. The first issue is very important because combating proliferation is vital to the security of all. Concerning the second issue, France attaches particular importance to strengthening specific measures to prevent and check proliferation. Let me mention a few proposals that we put forward: exports need to be controlled more effectively, proliferation activities have to be criminalized, or the development of proliferation-resistant technologies should be supported. Concerning the third issue it means the strengthening of the non-proliferation regime, France proposes several means: -) aiming at the universalization of the additional protocol; -) ensuring that the Agency continues to have sufficient human, financial and technical resources to fulfill its verification mission effectively; -) encouraging the IAEA to make full use of the authority available to it; -) enhancing the use of information relevant to the delivery of the IAEA mandate; and -) sharing more accurate information concerning the breaches of commitments that happen. The paper is followed by the slides of the presentation. (A.C.)

  11. The handbook of nuclear non-proliferation

    This report analyzed international non-proliferation regime preventing from spread of nuclear weapon. This report took review from the historical background of non-proliferation regime to the recent changes and current status. It is here divided into multilateral and bilateral regime. First of all, this report dealt four multilateral treaties concluded for international non-proliferation such as NPT, NWFZ, CTBT and others. And international organization and regimes concerned with non-proliferation are also analyzed focused on UN, IAEA, ZC and NSG, regional safeguards system and international conferences. In addition, this report reviewed the nuclear cooperation agreement related with Korea which is a important tool for bilateral regime

  12. Proliferation after the Iraq war; La proliferation apres la guerre d'Irak

    Daguzan, J.F

    2004-09-15

    This article uses the Iraq war major event to analyze the approach used by the US to fight against proliferation. It questions the decision and analysis process which has led to the US-British intervention and analyzes the consequences of the war on the proliferation of other countries and on the expected perspectives. Finally, the future of proliferation itself is questioned: do we have to fear more threat or is the virtuous circle of non-proliferation well started? (J.S.)

  13. Wnt3a is critical for endothelial progenitor cell-mediated neural stem cell proliferation and differentiation

    Du, Yibin; Zhang, Shuo; Yu, Tao; Du, Gongwen; Zhang, Hui; Yin, Zongsheng

    2016-01-01

    The present study aimed to determine whether co-culture with bone marrow-derived endothelial progenitor cells (EPCs) affects the proliferation and differentiation of spinal cord-derived neural stem cells (NSCs), and to investigate the underlying mechanism. The proliferation and differentiation of the NSCs were evaluated by an MTT cell proliferation and cytotoxicity assay, and immunofluorescence, respectively. The number of neurospheres and the number of β-tubulin III-positive cells were detected by microscopy. The wingless-type MMTV integration site family, member 3a (Wnt3a)/β-catenin signaling pathway was analyzed by western blot analysis and reverse transcription-quantitative polymerase chain reaction to elucidate the possible mechanisms of EPC-mediated NSC proliferation and differentiation. The results revealed that co-culture with EPCs significantly induced NSC proliferation and differentiation. In addition, co-culture with EPCs markedly induced the expression levels of Wnt3a and β-catenin and inhibited the phosphorylation of glycogen synthase kinase 3β (GSK-3β). By contrast, Wnt3a knockdown using a short hairpin RNA plasmid in the EPCs reduced EPC-mediated NSC proliferation and differentiation, accompanied by inhibition of the EPC-mediated expression of β-catenin, and its phosphorylation and activation of GSK-3β. Taken together, the findings of the present study demonstrated that Wnt3a was critical for EPC-mediated NSC proliferation and differentiation. PMID:27484039

  14. Cyclic mechanical strain-induced proliferation and migration of human airway smooth muscle cells: role of EMMPRIN and MMPs.

    Hasaneen, Nadia A; Zucker, Stanley; Cao, Jian; Chiarelli, Christian; Panettieri, Reynold A; Foda, Hussein D

    2005-09-01

    Airway smooth muscle (ASM) proliferation and migration are major components of airway remodeling in asthma. Asthmatic airways are exposed to mechanical strain, which contributes to their remodeling. Matrix metalloproteinase (MMP) plays an important role in remodeling. In the present study, we examined if the mechanical strain of human ASM (HASM) cells contributes to their proliferation and migration and the role of MMPs in this process. HASM were exposed to mechanical strain using the FlexCell system. HASM cell proliferation, migration and MMP release, activation, and expression were assessed. Our results show that cyclic strain increased the proliferation and migration of HASM; cyclic strain increased release and activation of MMP-1, -2, and -3 and membrane type 1-MMP; MMP release was preceded by an increase in extracellular MMP inducer; Prinomastat [a MMP inhibitor (MMPI)] significantly decreased cyclic strain-induced proliferation and migration of HASM; and the strain-induced increase in the release of MMPs was accompanied by an increase in tenascin-C release. In conclusion, cyclic mechanical strain plays an important role in HASM cell proliferation and migration. This increase in proliferation and migration is through an increase in MMP release and activation. Pharmacological MMPIs should be considered in the pursuit of therapeutic options for airway remodeling in asthma. PMID:16014803

  15. SGLT1 activity in lung alveolar cells of diabetic rats modulates airway surface liquid glucose concentration and bacterial proliferation

    Oliveira, Tales Lyra; Candeia-Medeiros, Návylla; Cavalcante-Araújo, Polliane M.; Melo, Igor Santana; Fávaro-Pípi, Elaine; Fátima, Luciana Alves; Rocha, Antônio Augusto; Goulart, Luiz Ricardo; Machado, Ubiratan Fabres; Campos, Ruy R.; Sabino-Silva, Robinson

    2016-01-01

    High glucose concentration in the airway surface liquid (ASL) is an important feature of diabetes that predisposes to respiratory infections. We investigated the role of alveolar epithelial SGLT1 activity on ASL glucose concentration and bacterial proliferation. Non-diabetic and diabetic rats were intranasally treated with saline, isoproterenol (to increase SGLT1 activity) or phlorizin (to decrease SGLT1 activity); 2 hours later, glucose concentration and bacterial proliferation (methicillin-resistant Sthaphylococcus aureus, MRSA and Pseudomonas aeruginosa, P. aeruginosa) were analyzed in bronchoalveolar lavage (BAL); and alveolar SGLT1 was analyzed by immunohistochemistry. BAL glucose concentration and bacterial proliferation increased in diabetic animals: isoproterenol stimulated SGLT1 migration to luminal membrane, and reduced (50%) the BAL glucose concentration; whereas phlorizin increased the BAL glucose concentration (100%). These regulations were accompanied by parallel changes of in vitro MRSA and P. aeruginosa proliferation in BAL (r = 0.9651 and r = 0.9613, respectively, Pearson correlation). The same regulations were observed in in vivo P. aeruginosa proliferation. In summary, the results indicate a relationship among SGLT1 activity, ASL glucose concentration and pulmonary bacterial proliferation. Besides, the study highlights that, in situations of pulmonary infection risk, such as in diabetic subjects, increased SGLT1 activity may prevent bacterial proliferation whereas decreased SGLT1 activity can exacerbate it. PMID:26902517

  16. Langerhans cell histiocytosis of the thyroid with multiple cervical lymph node involvement accompanying metastatic thyroid papillary carcinoma.

    Ceyran, A Bahar; Senol, Serkan; Bayraktar, Barış; Ozkanlı, Seyma; Cinel, Z Leyla; Aydın, Abdullah

    2014-01-01

    A 37-year-old male case was admitted with goiter. Ultrasonography of thyroid showed a 5 cm cystic nodule in the left lobe with a 1.5 cm solid component. Fine needle aspiration biopsy revealed atypia of undetermined significance or follicular lesion. The patient was operated on. The pathological diagnosis was reported as papillary thyroid carcinoma. The immunohistochemical examination showed multiple foci of Langerhans cell histiocytosis involving both lobes. The patient died due to cardiac arrest with respiratory causes in the early postoperative period. Langerhans cell histiocytosis is a rare primary condition which involves abnormal clonal proliferation of Langerhans cells in various tissues and organs. Thyroid involvement is infrequently seen. Although the etiology is unknown, genetic components may be linked to the disease. It is also associated with a family history of thyroid disease. Papillary thyroid carcinoma is the most common malignant epithelial tumor of the thyroid gland. Langerhans cell histiocytosis presenting with papillary thyroid carcinoma is rare. The privilege of our case is langerhans cell histiocytosis of the thyroid with multiple cervical lymph node involvement accompanying cervical lymph node metastatic thyroid papillary carcinoma. PMID:25349760

  17. Monitoring technology proliferation: an open source methodology for generating proliferation intelligence

    Green, Daniel M.

    1993-01-01

    Approved for public release; distribution is unlimited. This thesis develops a methodology to monitor technology proliferation. It is designed to provide proliferation intelligence on specific threat technologies and can be used to augment export controls or enhance counter proliferation initiatives. A high-te Lieutenant, United States Navy

  18. Director`s series on proliferation

    Bailey, K.C.; Price, M.E. [eds.

    1995-11-17

    This is an occasional publication of essays on the topics of nuclear, chemical, biological, and missile proliferation. The views represented are those of the author`s. Essay topics include: Nuclear Proliferation: Myth and Reality; Problems of Enforcing Compliance with Arms Control Agreements; The Unreliability of the Russian Officer Corps: Reluctant Domestic Warriors; and Russia`s Nuclear Legacy.

  19. Control of cell proliferation by Myc

    Bouchard, C; Staller, P; Eilers, M

    1998-01-01

    Myc proteins are key regulators of mammalian cell proliferation. They are transcription factors that activate genes as part of a heterodimeric complex with the protein Max. This review summarizes recent progress in understanding how Myc stimulates cell proliferation and how this might contribute to...

  20. Proliferation resistance of small modular reactors fuels

    In this paper the proliferation resistance of different types of Small Modular Reactors (SMRs) has been examined and classified with criteria available in the literature. In the first part of the study, the level of proliferation attractiveness of traditional low-enriched UO2 and MOX fuels to be used in SMRs based on pressurized water technology has been analyzed. On the basis of numerical simulations both cores show significant proliferation risks. Although the MOX core is less proliferation prone in comparison to the UO2 core, it still can be highly attractive for diversion or undeclared production of nuclear material. In the second part of the paper, calculations to assess the proliferation attractiveness of fuel in typical small sodium cooled fast reactor show that proliferation risks from spent fuel cannot be neglected. The core contains a highly attractive plutonium composition during the whole life cycle. Despite some aspects of the design like the sealed core that enables easy detection of unauthorized withdrawal of fissile material and enhances proliferation resistance, in case of open Non-Proliferation Treaty break-out, weapon-grade plutonium in sufficient quantities could be extracted from the reactor core

  1. Economic air policy for non-proliferation

    In today's world, arms proliferation is a major concern, posing a threat to international peace and security. While the concerted efforts of donor countries to formulate and pursue economic aid policies in such a way as to stem proliferation may not be decisive, they will surely contribute to a more stable international environment

  2. Safeguards and non-proliferation

    It deserves re-emphasizing that the first and most important obstacle to the proliferation of nuclear weapons is a matter of political judgement and determination. Safeguards cannot prevent a violation of obligations... any more than bank or company audits can prevent a misappropriation of funds. All they can do is expose infringements or arouse suspicions - in effect, sound the alarm. By submitting the whole of their nuclear energy sector to impartial international inspection, States can inspire great confidence on the part of the rest of the world in the exclusively peaceful nature of their programmes. Safeguards are today an essential precondition for imports of nuclear power technology, uranium fuel, and many different kinds of material for the nuclear energy sector. Without IAEA safeguards, the existing market in this sector would be unworkable. Potentially, the most important aspect of the acceptance of IAEA safeguards by nuclear-weapon States is that it shows their readiness to submit important installations within their territory to impartial inspection

  3. The challenges of nuclear proliferation

    The author of this article first outlines that the Non Proliferation Treaty (NPT) is a tool of domination used by nuclear powers: they can keep and even develop their own nuclear arsenal, while other countries who sign this treaty commit themselves not to try to acquire nuclear weapons. The USA and USSR kept on persuading various countries to sign this treaty, but eventually let some countries develop their military nuclear programme (Israel, Pakistan, or India). He evokes technical difficulties in the application of the Treaty, notably for the control of centrifugation activities. He outlines that the USA have now a dominant position with respect to this Treaty and its application, but that the Treaty remains a major safety element for the world. He evokes more recent and negative evolutions: the withdrawal of North Korea from the Treaty, the destruction of an Iraqi nuclear reactor by Israel (i.e. the destruction of a nuclear installation belonging to a country who signed the NPT by a country who did not sign it). He proposes an overview of the Iranian issue (history of the Iranian nuclear programme, of the nuclear crisis, of the still going on negotiations), and describes what could be the worst possible scenario

  4. North Korea: a mercenary proliferator?

    After having recalled that North Korea possesses a rather advanced ballistic programme which has been started in the 1970 with the Chinese support, that North Korea is the fourth world producer of ballistic missiles, the author outlines that this country has become a major proliferator as it exports this production to different States and non-State actors. He recalls the long history of relationships between North Korea and terrorist organisations (even during the Cold War), comments the current and major support of North Korea to Hamas and Hezbollah in Gaza and in Lebanon. These relationships are then related with those these both organisations have with Syria and Iran who are in fact the relays between them and North Korea. The author explains why Hamas and Hezbollah must buy their weapons to such a far country: Iran is submitted to international sanctions, Iran and Syria want to avoid being banned from the international community for selling weapon to a terrorist (or so-said) organisation, and prices are rather competitive. If North Korea is also submitted to international sanctions, weapon smuggling seems to be institutional in this country. The author finally briefly evokes the issue of chemical weapons: North Korea possesses few thousand tonnes of these weapons, and could export them to non-state organisations

  5. IPSN and the mastery of proliferation risks

    Since 20 years, the French Institute of Nuclear Protection and Safety (IPSN) works for the reinforcement of the efficiency of the actions against nuclear proliferation. This dossier takes stock of the means and action developed by the IPSN in this domain: nuclear and chemical proliferation: towards a reinforced mastery of risks (status of proliferation during the last 10 years, the aim of the international and national controls, the role of IPSN); nuclear proliferation: the technical control means of the IPSN (inspection, containment/surveillance of nuclear materials (PLUM and FUNE apparatuses), the cooperation between the IPSN and the Kurchatov institute (Russia)); the action of the IPSN in the application of international controls: example of chemistry (conventions, negotiations and expertise, inspections); appendixes: nuclear materials (declaration and permission thresholds), the different steps of the nuclear weapons non-proliferation policy. (J.S.)

  6. Nuclear non-proliferation and global security

    The book has 16 chapters grouped into three sections - Global Security and the Treaty on the Non-Proliferation of Nuclear Weapons -the Agenda; Nuclear Non-Proliferation Policies and Perspectives and the Treaty on the Non-Proliferation of Nuclear Weapons in Review. Apart from the Review of the Third NPT Conference all the articles were presented at the Conference on Global Security and the Future of the Non-Proliferation Treaty: a time for reassessment, held in May 1985. All are indexed separately. The Appendices include the Statement of the Treaty on the Non-Proliferation of Nuclear Weapons, a list of parties to the Treaty, those signing but not ratifying the Treaty and those neither signing nor ratifying and Appendix I and II of the Final Document of the Third Review Conference of the Parties to the NPT Treaty. (U.K.)

  7. Christoph Rothmann's discourse on the comet of 1585 an edition and translation with accompanying essays

    Granada, Miguel A; Jardine, Nicholas

    2014-01-01

    Christoph Rothmann's Discourse on the Comet of 1585 offers the first edition of the Latin treatise after it was published in 1619. It is accompanied by an English translation and a full introduction and commentary.

  8. Muscle progenitor cells proliferation doesn't sufficiently contribute to maintaining stretched soleus muscle mass during gravitational unloading

    Tarakina, M. V.; Turtikova, O. V.; Nemirovskaya, T. L.; Kokontcev, A. A.; Shenkman, B. S.

    Skeletal muscle work hypertrophy is usually connected with muscle progenitor satellite cells (SC) activation with subsequent incorporation of their nuclei into myofibers. Passive stretch of unloaded muscle was earlier established to prevent atrophic processes and is accompanied by enhanced protein synthesis. We hypothesized that elimination of SC proliferation capacity by γ-irradiation would partly avert stretched muscle fiber capability to maintain their size under the conditions of gravitational unloading. To assess the role of muscle progenitor (satellite) cells in development of passive stretch preventive effect SC proliferation was suppressed by local exposing to ionized radiation (2500 rad), subsequent hindlimb suspension or hindlimb suspension with concomitant passive stretch were carried out. Reduction of myofiber cross-sectional area and decrease in myonuclei number accompanying unloaded muscle atrophy were completely abolished by passive stretch both in irradiated and sham-treated animals. We conclude that SC did not make essential contribution to passive stretch preventive action under the conditions of simulated weightlessness.

  9. Which future for nuclear counter-proliferation?

    Dealing with the case of nuclear weapons possessed by nuclear states (but not eventually by terrorists), the author first identifies the constants of counter-proliferation: it is linked to interest conflicts between those who try to preserve their monopoly and those who try to acquire a new weapon either because of a threat or for reasons of regional prestige, the evolution from use to deterrence, the appearance of new actors after the USA and Russia, the role of nuclear tactical weapons, and the future of Russian weapons and know-how. He presents the international counter-proliferation context: the Non Proliferation Treaty (NPT), the IAEA and its controls, the Nuclear Supplier Group (NSG), the nuclear-free zones, the Comprehensive Test Ban Treaty (CTBT), the Missile Technology Control Regime (MTCR). He describes how and why proliferation occurs: uranium enrichment and plutonium technology, political reasons in different parts of the world. Then, he gives an overview of the proliferation status by commenting the cases of Israel, Iraq, India, Pakistan, North Korea, and Iran. He discusses the future of proliferation (involved countries, existence of a nuclear black market) and of counter-proliferation as far as Middle-East and North Korea are concerned. He tries finally to anticipate the consequences for nuclear deterrence strategy, and more particularly for Europe and France

  10. Non-proliferation and multinational enterprises

    The paper supplements CC/WG.2/9 in presenting the Japanese delegation's contribution in the areas of non-proliferation and multi-national enterprises. The paper questions whether multinational enrichment enterprises would constitute a significant non-proliferation factor, noting that the nature of the venture might create a potential for the dissemination of sensitive information. The paper also argues that a multi-national venture which was not economically competitive (with national facilities) would have questionable viability. The conclusion is that non-proliferation advantages, if any, would be a result, not an objective of such a venture

  11. Sox11 modulates neocortical development by regulating the proliferation and neuronal differentiation of cortical intermediate precursors

    Yongzhe Li; Qingsong Li; Jianjiao Wang; Yongri Zheng; Yan Zhao; Mian Guo; Yang Li; Qiuli Bao; Yu Zhang; Lizhuang Yang

    2012-01-01

    Neural precursor cells play important roles in the neocortical development,but the mechanisms of neural progenitor proliferation,neuronal differentiation,and migration,as well as patterning are still unclear.Sox11,one of SoxC family members,has been reported to be essential for embryonic and adult neurogenesis.But there is no report about the roles of Sox11 in corticogenesis.In order to investigate Sox11 function during cortical development,loss of function experiment was performed in this study.Knockdown of Sox11 by Sox11 siRNA constructs resulted in a diminished neuronal differentiation,but enhanced proliferation of intermediate progenitors.Accompanied with the high expression of Sox11 in the postmitotic neurons,but low expression of Sox11 in the dividing neural progenitors,all the observations indicate that Sox11 induces neuronal differentiation during the neocortical development.

  12. Handbook for nuclear non-proliferation

    Lee, Byung Wook; Oh, Keun Bae; Lee, Kwang Seok; Lee, Dong Jin; Ko, Han Seok

    1997-05-01

    This book analyzed international non-proliferation regime preventing from spread of nuclear weapon. This book took review from the historical background of non-proliferation regime to the recent changes and status. The regime, here, is divided into multilateral and bilateral regime. First of all, this book reports four multilateral treaties concluded for non-proliferation such as NPT, NWFZ, CTBT and others. Secondly, international organization and regimes concerned with non-proliferation are analyzed with emphasis of UN, IAEA, ZC and NSG, Regional Safeguards System and international conference. Finally, this book report the current circumstances of nuclear cooperation agreement related with Korea which is an important means for bilateral regime. (author). 13 tabs., 2 figs.

  13. Handbook for nuclear non-proliferation

    This book analyzed international non-proliferation regime preventing from spread of nuclear weapon. This book took review from the historical background of non-proliferation regime to the recent changes and status. The regime, here, is divided into multilateral and bilateral regime. First of all, this book reports four multilateral treaties concluded for non-proliferation such as NPT, NWFZ, CTBT and others. Secondly, international organization and regimes concerned with non-proliferation are analyzed with emphasis of UN, IAEA, ZC and NSG, Regional Safeguards System and international conference. Finally, this book report the current circumstances of nuclear cooperation agreement related with Korea which is an important means for bilateral regime. (author). 13 tabs., 2 figs

  14. Canada's nuclear non-proliferation policy

    Canada's non-proliferation safeguards policy has two objectives: 1) to promote a more effective and comprehensive international non-proliferation regime; and 2) to ensure that Canadian nuclear exports will not be used for any nuclear explosive purpose. By emphasizing the key role of the Non-Proliferation Treaty, promoting reliance upon and improvements in the IAEA safeguards system, treating nuclear weapon and non-weapon states alike, and working for new approaches covering reprocessing, Canada promotes attainment of the first objective. The second is served through the network of bilateral nuclear agreements that Canada has put into place with its partners. The Canadian objective in post-INFCE forums is to persuade the international community to devise a more effective and comprehensive non-proliferation regime into which Canada and other suppliers may subsume their national requirements

  15. Erythropoietin -induced proliferation of gastric mucosal cells

    Itoh, Kazuro; Sawasaki, Yoshio; Takeuchi, Kyoko; Kato, Shingo; Imai, Nobuhiro; Kato, Yoichiro; Shibata, Noriyuki; KOBAYASHI, MAKIO; Moriguchi, Yoshiyuki; Higuchi, Masato; Ishihata, Fumio; Sudoh, Yushi; Miura, Soichiro

    2006-01-01

    AIM: To analyze the localization of erythropoietin receptor on gastric specimens and characterize the effects of erythropoietin on the normal gastric epithelial proliferation using a porcine gastric epithelial cell culture model.

  16. Proliferation resistance features in nuclear reactor designs

    Full text: The presentation gives an overview of the fundamental principles of non-proliferation of nuclear materials and technologies in the process of designing the nuclear reactors. The nuclear power engineering includes the activities involving the risk of proliferation of nuclear weapons (such as separation of uranium isotopes (enrichment), long-term storage of irradiated fuel, reprocessing of irradiated fuel by means of separation of plutonium and/or uranium wherefrom, storage of separated fissile materials). Proliferation resistance can be defined as the characteristic of a given nuclear power system which would prevent change-over or unauthorized production and use of the nuclear materials or technologies intended to possession of nuclear weapons or other nuclear explosives. The basic principles of non-proliferation as formulated in the frame of IAEA-sponsored international project INPRO have been analyzed for their relevance in designing the innovative nuclear power systems based on lead-cooled fast reactors. (author)

  17. Nuclear non-proliferation and global security

    The nuclear non-proliferation treaty is currently undergoing its third formal review by its signatories. They are reviewing the treaty, assessing its effectiveness and considering how it might better be implemented. This book anticipates the work of the treaty reviewers and considers the issues facing them. It examines the whole range of issues connected with nuclear non-proliferation and the treaty. It looks at non-proliferation from the point of view of nuclear nations, non-nuclear nations and the nuclear industry. It assesses the work of the international monitoring bodies and reconsiders the place of non-proliferation in the changing balance of global nuclear power. It concludes by discussing the way forward

  18. Proliferation resistance features in nuclear reactor designs

    The paper presents a review of the main principles for technologies and materials protection from unauthorized proliferation and application to be considered in nuclear reactors designing. Nuclear power features certain operations sensitive to nuclear weapons proliferation (such as separation of uranium isotopes (enrichment), long storage of spent fuel, processing of spent fuel, plutonium and/or uranium recovery from spent fuel, storage of recovered fissile materials). Proliferation resistance is defined as a nuclear energy system characteristic that impedes the diversion or undeclared production of nuclear material, or misuse of technology with the purpose of acquiring nuclear weapons or other nuclear explosive devices. The basic principles of non-proliferation established in the INPRO international project sponsored by IAEA have been discussed as implemented for designing of the innovative nuclear energy systems based on fast lead-cooled nuclear reactors

  19. Nuclear non-proliferation: a reference handbook

    This handbook aims to give data on the current state of non-proliferation in the world. In Part One articles give an overview on non-proliferation; its history, the review conferences, nuclear power nuclear weapons, nuclear safeguards, nuclear trade control, security and physical protection of nuclear materials, nuclear weapon free zones, arms control and disarmament, nuclear testing and the current state of civil nuclear power. Even articles are indexed separately. Part Two includes the texts of the non-proliferation treaties, nuclear weapons treaties, United Nations resolutions and all the agreements and declarations relevant to the operation of non-proliferation in the early 1990s. Part Two which also includes a bibliography takes up more than three quarters of the book. (UK)

  20. Pioglitazone promotes preadipocyte proliferation by downregulating p16{sup Ink4a}

    Hasan, Arif U. [Department of Cardiorenal Cerebrovascular Medicine, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793 (Japan); Ohmori, Koji, E-mail: komori@med.kagawa-u.ac.jp [Department of Cardiorenal Cerebrovascular Medicine, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793 (Japan); Hashimoto, Takeshi [Department of Cardiovascular Physiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793 (Japan); Kamitori, Kazuyo; Hirata, Yuko [Department of Cell Physiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793 (Japan); Ishihara, Yasuhiro; Okamoto, Naoko; Noma, Takahisa [Department of Cardiorenal Cerebrovascular Medicine, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793 (Japan); Kosaka, Hiroaki [Department of Cardiovascular Physiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793 (Japan); Tokuda, Masaaki [Department of Cell Physiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793 (Japan); Kohno, Masakazu [Department of Cardiorenal Cerebrovascular Medicine, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793 (Japan)

    2011-07-29

    Highlights: {yields} Mechanisms for preadipocyte hyperplasia by pioglitazone, a PPAR{gamma} agonist, are shown. {yields} Pioglitazone promotes cell-cycle of 3T3-L1 preadipocytes and increases their number. {yields} Pioglitazone downregulates a cyclin dependent kinase inhibitor, p16{sup Ink4a}. {yields} PPAR{gamma} transrepresses p16{sup Ink4a} gene in preadipocytes, which pioglitazone enhances. -- Abstract: Pioglitazone, a synthetic ligand of peroxisome proliferator-activated receptor (PPAR){gamma}, causes preadipocyte proliferation through a mechanism which still remains elusive. Here, to address the mechanism, we investigated the effects of PPAR{gamma} and pioglitazone on the kinetics of cyclin-dependent kinase inhibitors, especially with p16{sup Ink4a} (p16) centered, by employing 3T3-L1 preadipocytes. Pioglitazone promoted preadipocyte proliferation by increasing S and G{sub 2}/M cell-cycle entry, which was accompanied by decreased p16 mRNA expression. PPAR{gamma} overexpression along with the luciferase reporter assay confirmed that PPAR{gamma} was crucial for the downregulation of p16 mRNA transcription, and that the action was augmented by pioglitazone. Thus, pioglitazone exerted cell-cycle dependent promoting effect on preadipocyte proliferation, of which mechanisms include p16-downregulation through PPAR{gamma}.

  1. Collagen Promotes Higher Adhesion, Survival and Proliferation of Mesenchymal Stem Cells.

    Chinnapaka Somaiah

    Full Text Available Mesenchymal stem cells (MSC can differentiate into several cell types and are desirable candidates for cell therapy and tissue engineering. However, due to poor cell survival, proliferation and differentiation in the patient, the therapy outcomes have not been satisfactory. Although several studies have been done to understand the conditions that promote proliferation, differentiation and migration of MSC in vitro and in vivo, still there is no clear understanding on the effect of non-cellular bio molecules. Of the many factors that influence the cell behavior, the immediate cell microenvironment plays a major role. In this context, we studied the effect of extracellular matrix (ECM proteins in controlling cell survival, proliferation, migration and directed MSC differentiation. We found that collagen promoted cell proliferation, cell survival under stress and promoted high cell adhesion to the cell culture surface. Increased osteogenic differentiation accompanied by high active RHOA (Ras homology gene family member A levels was exhibited by MSC cultured on collagen. In conclusion, our study shows that collagen will be a suitable matrix for large scale production of MSC with high survival rate and to obtain high osteogenic differentiation for therapy.

  2. Role of PKC isozymes in low-power light-stimulated proliferation of cultured skin cells

    Grossman, Nili; Kleitman, Vered; Meller, Julia; Kaufmann, Roland; Akgun, Nermin; Ruck, Angelika; Livneh, Etta; Lubart, Rachel

    2000-11-01

    Exposure of cultured skin cells to low power visible light leads to a transiently stimulated proliferation. Facilitation of this response requires the presence of active PKC, elevation of intracellular calcium, and involves reactive oxygen species. In the present study, the role of PKC(alpha) and PCK(eta) was examined using paired murine fibroblasts, differing in the level of these isozymes expression. The ability of the cells to respond to low power UVA light or HeNe laser by stimulated proliferation was correlated with an active state or overexpression of PKC(alpha) , but not PKC(eta) . A parallel response was obtained in cells that were loaded with A1PcS4 before photosensitization. Whenever this latter treatment caused a light-stimulated inhibition, it was accompanied by the intracellular calcium and photosensitizer dynamics typical of the effect of PDT on rate epithelial cells. Accordingly, added antioxidants that suppressed light-stimulated proliferation also suppressed this light-stimulated inhibition. The model systems employed in this study are the first to demonstrate the specific effect of PKC isozymes on light-stimulated proliferation, in relation to oxidative stress, and indicate their dual role in light-tissue interaction.

  3. Pyruvate carboxylase is critical for non-small-cell lung cancer proliferation.

    Sellers, Katherine; Fox, Matthew P; Bousamra, Michael; Slone, Stephen P; Higashi, Richard M; Miller, Donald M; Wang, Yali; Yan, Jun; Yuneva, Mariia O; Deshpande, Rahul; Lane, Andrew N; Fan, Teresa W-M

    2015-02-01

    Anabolic biosynthesis requires precursors supplied by the Krebs cycle, which in turn requires anaplerosis to replenish precursor intermediates. The major anaplerotic sources are pyruvate and glutamine, which require the activity of pyruvate carboxylase (PC) and glutaminase 1 (GLS1), respectively. Due to their rapid proliferation, cancer cells have increased anabolic and energy demands; however, different cancer cell types exhibit differential requirements for PC- and GLS-mediated pathways for anaplerosis and cell proliferation. Here, we infused patients with early-stage non-small-cell lung cancer (NSCLC) with uniformly 13C-labeled glucose before tissue resection and determined that the cancerous tissues in these patients had enhanced PC activity. Freshly resected paired lung tissue slices cultured in 13C6-glucose or 13C5,15N2-glutamine tracers confirmed selective activation of PC over GLS in NSCLC. Compared with noncancerous tissues, PC expression was greatly enhanced in cancerous tissues, whereas GLS1 expression showed no trend. Moreover, immunohistochemical analysis of paired lung tissues showed PC overexpression in cancer cells rather than in stromal cells of tumor tissues. PC knockdown induced multinucleation, decreased cell proliferation and colony formation in human NSCLC cells, and reduced tumor growth in a mouse xenograft model. Growth inhibition was accompanied by perturbed Krebs cycle activity, inhibition of lipid and nucleotide biosynthesis, and altered glutathione homeostasis. These findings indicate that PC-mediated anaplerosis in early-stage NSCLC is required for tumor survival and proliferation. PMID:25607840

  4. Regulation of P450 4A expression by peroxisome proliferator activated receptors

    The induction of P450 4A enzymes by peroxisome proliferators (PPs) and fatty acids is mediated by the peroxisome proliferator activated receptor α (PPARα) that binds to response elements in target genes as a heterodimer with the retinoid X receptor (RXR). The consensus sequence recognized by PPAR/RXR heterodimers, contains an imperfect direct repeat of two nuclear receptor binding motifs separated by a single nucleotide. This repeat is preceded by a conserved A/T rich sequence that is required for function. In mice, chronic exposure to PPs results in PPARα mediated liver hypertrophy, hyperplasia and carcinogenesis accompanied by a proliferation of peroxisomes. In contrast, humans exhibit a reduced sensitivity to PP pathogenesis. This could reflect >10-fold lower PPARα levels relative to mice as well as differences in targeted genes. In order to identify PPAR responsive human genes, the human hepatoma cell line, HepG2, was engineered to express increased levels of PPARα. Several genes encoding rate-limiting enzymes and branch points in ketone body formation are regulated by PPARα in these cells. In contrast, significant induction by PP is not evident for peroxisomal fatty acid oxidation that is associated with peroxisome proliferation in mice. Human P450 4A11 is not expressed in dividing cultures of cells with enhanced PPARα levels, but it is expressed in confluent cultures expressing elevated amounts of PPARα

  5. Pioglitazone promotes preadipocyte proliferation by downregulating p16Ink4a

    Highlights: → Mechanisms for preadipocyte hyperplasia by pioglitazone, a PPARγ agonist, are shown. → Pioglitazone promotes cell-cycle of 3T3-L1 preadipocytes and increases their number. → Pioglitazone downregulates a cyclin dependent kinase inhibitor, p16Ink4a. → PPARγ transrepresses p16Ink4a gene in preadipocytes, which pioglitazone enhances. -- Abstract: Pioglitazone, a synthetic ligand of peroxisome proliferator-activated receptor (PPAR)γ, causes preadipocyte proliferation through a mechanism which still remains elusive. Here, to address the mechanism, we investigated the effects of PPARγ and pioglitazone on the kinetics of cyclin-dependent kinase inhibitors, especially with p16Ink4a (p16) centered, by employing 3T3-L1 preadipocytes. Pioglitazone promoted preadipocyte proliferation by increasing S and G2/M cell-cycle entry, which was accompanied by decreased p16 mRNA expression. PPARγ overexpression along with the luciferase reporter assay confirmed that PPARγ was crucial for the downregulation of p16 mRNA transcription, and that the action was augmented by pioglitazone. Thus, pioglitazone exerted cell-cycle dependent promoting effect on preadipocyte proliferation, of which mechanisms include p16-downregulation through PPARγ.

  6. Hydrogen sulfide lowers proliferation and induces protective autophagy in colon epithelial cells.

    Ya C Wu

    Full Text Available Hydrogen sulfide (H(2S is a gaseous bacterial metabolite that reaches high levels in the large intestine. In the present study, the effect of H(2S on the proliferation of normal and cancerous colon epithelial cells was investigated. An immortalized colon epithelial cell line (YAMC and a panel of colon cancer cell lines (HT-29, SW1116, HCT116 were exposed to H(2S at concentrations similar to those found in the human colon. H(2S inhibited normal and cancerous colon epithelial cell proliferation as measured by MTT assay. The anti-mitogenic effect of H(2S was accompanied by G(1-phase cell cycle arrest and the induction of the cyclin-dependent kinase inhibitor p21(Cip. Moreover, exposure to H(2S led to features characteristic of autophagy, including increased formation of LC3B(+ autophagic vacuoles and acidic vesicular organelles as determined by immunofluorescence and acridine orange staining, respectively. Abolition of autophagy by RNA interference targeting Vps34 or Atg7 enhanced the anti-proliferative effect of H(2S. Further mechanistic investigation revealed that H(2S stimulated the phosphorylation of AMP-activated protein kinase (AMPK and inhibited the phosphorylation of mammalian target of rapamycin (mTOR and S6 kinase. Inhibition of AMPK significantly reversed H(2S-induced autophagy and inhibition of cell proliferation. Collectively, we demonstrate that H(2S inhibits colon epithelial cell proliferation and induces protective autophagy via the AMPK pathway.

  7. Sweden and the making of nuclear non-proliferation: from indecision to assertiveness

    Dassen, L. van [Uppsala Univ. (Sweden). Dept. of Peace and Conflict Research

    1998-03-01

    Swedish research on nuclear weapons started at a modest scale in 1945 but was soon expanded. By the early 1950s the research programme started to face some of the problems that were going to accompany it for the rest of its life: different priorities and cost-estimates were made by the sectors that wanted to develop nuclear energy and those working on the bomb. Moreover, an introduction of nuclear weapons would lead to a major redistribution of resources to the disadvantage of the navy and army. The public and political debates intensified during the 1950s and culminated in 1960. At first, pro-nuclear voices had been strongest but were soon challenged by interest groups, unions and peace movements. 1960, a committee within the government had established a compromise: Nuclear weapons research for production of weapons would be terminated, while research on the consequences of nuclear weapons would continue. It was a cosmetic decision that could cover for a continued research on weapons design. Nevertheless, there are some general qualities from the debates that indicate why the outcome was that Sweden signed the NPT in 1968. First, the number of interested persons, groups movements and party politicians engaged in the issue increased every time the issue came up. Secondly, the segments of society that supported the nuclear option remained roughly the same. No strong movements rallied to the defence of this position. On the other hand, the anti-nuclear wing received more and more followers. Third, there was a marked tendency by virtually all actors (except the military) to include every sign of progress in international disarmament and non-proliferation efforts as arguments against Swedish proliferation. Since 1968, the non-proliferation choice has ben manifested through Sweden``s adherence to the NPT and this has been accompanied by a strong commitment to other non-proliferation initiatives. Refs.

  8. Sweden and the making of nuclear non-proliferation: from indecision to assertiveness

    Swedish research on nuclear weapons started at a modest scale in 1945 but was soon expanded. By the early 1950s the research programme started to face some of the problems that were going to accompany it for the rest of its life: different priorities and cost-estimates were made by the sectors that wanted to develop nuclear energy and those working on the bomb. Moreover, an introduction of nuclear weapons would lead to a major redistribution of resources to the disadvantage of the navy and army. The public and political debates intensified during the 1950s and culminated in 1960. At first, pro-nuclear voices had been strongest but were soon challenged by interest groups, unions and peace movements. 1960, a committee within the government had established a compromise: Nuclear weapons research for production of weapons would be terminated, while research on the consequences of nuclear weapons would continue. It was a cosmetic decision that could cover for a continued research on weapons design. Nevertheless, there are some general qualities from the debates that indicate why the outcome was that Sweden signed the NPT in 1968. First, the number of interested persons, groups movements and party politicians engaged in the issue increased every time the issue came up. Secondly, the segments of society that supported the nuclear option remained roughly the same. No strong movements rallied to the defence of this position. On the other hand, the anti-nuclear wing received more and more followers. Third, there was a marked tendency by virtually all actors (except the military) to include every sign of progress in international disarmament and non-proliferation efforts as arguments against Swedish proliferation. Since 1968, the non-proliferation choice has ben manifested through Sweden''s adherence to the NPT and this has been accompanied by a strong commitment to other non-proliferation initiatives

  9. Nitrofen suppresses cell proliferation and promotes mitochondriamediated apoptosis in type Ⅱ pneumocytes

    Qiang-song TONG; Li-duan ZHENG; Shao-tao TANG; Guo-song JIANG; Qing-lan R UAN; Fu-qing ZENC; Ji-hua DONG

    2007-01-01

    Aim: To characterize the molecular mechanisms of nitrofen-induced pulmonary hypoplasia. Methods: After administration of nitrofen to cultured type H A549 pneumocytes, cell proliferation and DNA synthesis were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetry, colony forma-tion assay, flow cytometry and [3H]-thymidine incorporation assay. Apoptosis was measured by terminal transferase-mediated dUTP nick-end-labeling, acridine orange-ethidium bromide staining and flow cytometry. Expression of proliferating cell nuclear antigen (PCNA) and apoptosis-related genes was assayed by immunofluorescence, RT-PCR and Western blot. Results: Nitrofen inhibited the cell proliferation of A549 cells in a dose- and time-dependent manner, accompa-nied by downregulation of PCNA. As a result, the DNA synthesis of nitrofen-treated A549 cells decreased, while cell cycle was arrested at G0/G1 phase. Moreover,nitrofen induced apoptosis of A549 cells, which was not abolished by Z-Val-Aia-Asp(OCH3)- fluoromethylketone. In addition, nitrofen decreased the expression of Bcl-XL, but not of Bcl-2, Bax, and Bak, resulting in a loss of mitochondrial membrane potential and the nuclear translocation of apoptosis-inducing factor (AIF). Meanwhile, nitrofen strongly activated the p38 mitogen-activated protein kinase (p38-MAPK). Pretreatment of cells with SB203580 (5 μmol/L) blocked nitrofen-induced phosphorylation of p38-MAPK and abolished nitrofen-induced AIF translocation and apoptosis in A549 cells. Conclusion: Nitrofen suppresses the proliferation of cultured type Ⅱ pneumocytes accompanied by the downregulation of PCNA, and induces mitochondria-mediated apoptosis involv-ing the activation of p38-MAPK.

  10. Adult cardiac fibroblast proliferation is modulated by calcium/calmodulin-dependent protein kinase II in normal and hypertrophied hearts.

    Martin, Tamara P; Lawan, Ahmed; Robinson, Emma; Grieve, David J; Plevin, Robin; Paul, Andrew; Currie, Susan

    2014-02-01

    Increased adult cardiac fibroblast proliferation results in an increased collagen deposition responsible for the fibrosis accompanying pathological remodelling of the heart. The mechanisms regulating cardiac fibroblast proliferation remain poorly understood. Using a minimally invasive transverse aortic banding (MTAB) mouse model of cardiac hypertrophy, we have assessed fibrosis and cardiac fibroblast proliferation. We have investigated whether calcium/calmodulin-dependent protein kinase IIδ (CaMKIIδ) regulates proliferation in fibroblasts isolated from normal and hypertrophied hearts. It is known that CaMKIIδ plays a central role in cardiac myocyte contractility, but nothing is known of its role in adult cardiac fibroblast function. The MTAB model used here produces extensive hypertrophy and fibrosis. CaMKIIδ protein expression and activity is upregulated in MTAB hearts and, specifically, in cardiac fibroblasts isolated from hypertrophied hearts. In response to angiotensin II, cardiac fibroblasts isolated from MTAB hearts show increased proliferation rates. Inhibition of CaMKII with autocamtide inhibitory peptide inhibits proliferation in cells isolated from both sham and MTAB hearts, with a significantly greater effect evident in MTAB cells. These results are the first to show selective upregulation of CaMKIIδ in adult cardiac fibroblasts following cardiac hypertrophy and to assign a previously unrecognised role to CaMKII in regulating adult cardiac fibroblast function in normal and diseased hearts. PMID:23881186

  11. Ghrelin inhibits proliferation and increases T-type Ca2+ channel expression in PC-3 human prostate carcinoma cells

    Research highlights: → Ghrelin decreases prostate carcinoma PC-3 cells proliferation. → Ghrelin favors apoptosis in PC-3 cells. → Ghrelin increase in intracellular free Ca2+ levels in PC-3 cells. → Grelin up-regulates expression of T-type Ca2+ channels in PC-3 cells. → PC-3 cells express T-channels of the CaV3.1 and CaV3.2 subtype. -- Abstract: Ghrelin is a multifunctional peptide hormone with roles in growth hormone release, food intake and cell proliferation. With ghrelin now recognized as important in neoplastic processes, the aim of this report is to present findings from a series of in vitro studies evaluating the cellular mechanisms involved in ghrelin regulation of proliferation in the PC-3 human prostate carcinoma cells. The results showed that ghrelin significantly decreased proliferation and induced apoptosis. Consistent with a role in apoptosis, an increase in intracellular free Ca2+ levels was observed in the ghrelin-treated cells, which was accompanied by up-regulated expression of T-type voltage-gated Ca2+ channels. Interestingly, T-channel antagonists were able to prevent the effects of ghrelin on cell proliferation. These results suggest that ghrelin inhibits proliferation and may promote apoptosis by regulating T-type Ca2+ channel expression.

  12. Nuclear Proliferation and Authority in World Politics

    Brian Frederking; Kaitlyne Motl; Nishant Timilsina

    2009-01-01

    We apply the “security-hierarchy paradox” to nuclear proliferation. Global security requires a certain amount of hierarchy. A world in which no nuclear proliferation rules exist to constrain states, for example, would not be secure. Global security requires legitimate and authoritative rules, which we define as rules that are mutually negotiated, binding to all andwhich provide a stable social order. Too much hierarchy, however, amounts to coercion andundermines global security. Rules that ar...

  13. To judge the degree of proliferation resistance

    This paper identified the Co-Chairman's proposals for assessing the degree of proliferation resistance. It identifies nine assessment factors which have been suggested in INFCE WG4 discussions and in the open literature as appropriate. These nine assessment factors are then considered in respect to their relative importance to the question of proliferation resistance. The paper is supported by an Appendix which gives selected extracts from the literature where the various assessment factors are discussed

  14. Nuclear power and the proliferation issue

    The purpose of the lecture is to discuss nuclear proliferation, analyse which problems are real and which are a misapprehension, and to suggest a way forward which retains the benefits of nuclear power while providing a more certain protection against undesirable proliferation. After an introductory section the lecture continues under the following headings: plutonium production and accessibility; the use of plutonium; fast reactor fuel; the interim period; conclusions. (U.K.)

  15. Proliferating trichilemmal tumor of the nose *

    Aristóteles Rosmaninho; Mónica Caetano; Ana de Oliveira; Teresa Pinto de Almeida; Manuela Selores; Rosário Alves

    2012-01-01

    Proliferating trichilemmal tumor is a rare tumor originating in the external root sheath, that is usually found in the scalp of middle-aged or elderly females. Its histologic appearance may not correlate with its clinical behavior. In addition, there are no guidelines available for the treatment of these tumors, making its management a challenge for physicians. We report the case of a 53 year-old woman with a proliferating trichilemmal tumor on her nose, which is a very uncommon location for ...

  16. Skin Cell Proliferation Stimulated by Microneedles

    Liebl, Horst; Luther C. Kloth

    2012-01-01

    A classical wound may be defined as a disruption of tissue integrity. Wounds, caused by trauma from accidents or surgery, that close via secondary intention rely on the biological phases of healing, i.e., hemostasis, inflammation, proliferation, and remodeling (HIPR). Depending on the wound type and severity, the inflammation phase begins immediately after injury and may last for an average of 7–14 days. Concurrent with the inflammation phase or slightly delayed, cell proliferation is stimula...

  17. Strengthening the nuclear non-proliferation regime

    Although the nuclear non-proliferation regime has enjoyed considerable success, today the regime has never been under greater threat. Three states have challenged the objectives of the NPT, and there is a technology challenge - the spread of centrifuge enrichment technology and know-how. A major issue confronting the international community is, how to deal with a determined proliferator? Despite this gloomy scenario, however, the non-proliferation regime has considerable strengths - many of which can be developed further. The regime comprises complex interacting and mutually reinforcing elements. At its centre is the NPT - with IAEA safeguards as the Treaty's verification mechanism. Important complementary elements include: restraint in the supply and the acquisition of sensitive technologies; multilateral regimes such as the CTBT and proposed FMCT; various regional and bilateral regimes; the range of security and arms control arrangements outside the nuclear area (including other WMD regimes); and the development of proliferation-resistant technologies. Especially important are political incentives and sanctions in support of non-proliferation objectives. This paper outlines some of the key issues facing the non-proliferation regime

  18. Supporting non proliferation and global security efforts

    CEA contributes as a major actor of France's action against nuclear proliferation and to the strengthening of nuclear security at national level as European and International levels, in particular through the support of the IAEA activities in nuclear non proliferation with the French Support Programme for the IAEA safeguards system and security with the contribution to the IAEA Nuclear Security Plan and cooperation projects with the European Commission. The CEA is a French government funded technological research organization, organized around 5 branches: Nuclear Energy, Technological Researches, Defence (DAM), Material Sciences and Life Sciences. Within the scope of its activities, CEA covers most of the research areas and techniques in nuclear non-proliferation and security. The CEA is also the advisor of the French Government on nuclear policy. Treaty monitoring and the development and implementation of non proliferation and global security programs is an important mission of DAM which rely on nuclear weapons manufacture and past testing experience. The programmes on non proliferation and global security carried out to fulfil DAM's mission cover the following areas: development of monitoring and detection methods and equipments, country profiles and nuclear stockpiles assessment, arms control instruments, proliferation resistance of nuclear fuel cycle, monitoring of nuclear tests, operation and maintenance of national detection capabilities and contribution to CTBT verification systems. (A.C.)

  19. Managing Proliferation Issues with Iran

    particular, will continue to play a vital role in determining the extent to which Iran is able to pursue WMD options. Without a fundamental change in the regional security environment, however, there is little reason to expect changes in Iranian WMD and missile policies, and the United States, acting alone and short of war, cannot prevent Iran from ultimately developing WMD and delivery systems. Furthermore, U.S. policies that take a tougher line with Russia, China and North Korea are not likely to lead to more restraint among these potential sources of WMD and missile technology. In the absence of engagement with Iran, unilateral U.S. economic sanctions will remain the principal, if flawed, U.S. policy tool for seeking to prevent Iran from acquiring WMD. The rationale is that by discouraging trade and investment, particularly in Iran's energy sector, the government of Iran will have less revenue to pursue proliferation. Without broad international support for economic isolation, however, such an effort may hinder Iran's WMD programs, though it cannot block them. Finally, options are needed to deal with major failures in nonproliferation efforts. These options include measures to deter Iranian use of WMD, to defend against their use if deterrence fails, and to destroy Iranian WMD capabilities should the need arise

  20. CCR1, an enzyme required for lignin biosynthesis in Arabidopsis, mediates cell proliferation exit for leaf development

    Xue, Jingshi; Luo, Dexian; Xu, Deyang;

    2015-01-01

    After initiation, leaves first undergo rapid cell proliferation. During subsequent development, leaf cells gradually exit the proliferation phase and enter the expansion stage, following a basipetally ordered pattern starting at the leaf tip. The molecular mechanism directing this pattern of leaf...... intermediate in lignin biosynthesis. FeA is known to have antioxidant activity, and the levels of reactive oxygen species (ROS) in ccr1 were markedly reduced. We also characterized another double mutant in CAFFEIC ACID O-METHYLTRANSFERASE (comt) and CAFFEOYL CoA 3-O-METHYLTRANSFERASE (ccoaomt), in which the FeA...... level was dramatically reduced. Cell proliferation in comt ccoaomt leaves was decreased, accompanied by elevated ROS levels, and the mutant phenotypes were partially rescued by treatment with FeA or another antioxidant (N-acetyl-L-cysteine). Taken together, our results suggest that CCR1, FeA and ROS...

  1. Pedagogical guidelines for educational accompaniment for grieving to adults with intellectual disabilities

    Pablo RODRÍGUEZ HERRERO

    2013-11-01

    Full Text Available It is needed a foundation and some orientations to act in loss and grief situations with adults with intellectual disabilities. In this article, through a relevant literature review, we based the educational accompaniment as a pedagogic methodology of support with three principal elements: a The conception of grief from its formative potential, b The prevention of disorders associated to grief complications, c The pedagogic intervention preferably from the tutorship or from the actuation of educators or professionals near to intellectual disabilities people. The educational accompaniment model is a proposal that can be placed on humanist models of grief since it considers both the characteristics of the grieving process and the formative possibilities of its elaboration. The article’s conclusions present some of the benefits of the educational accompaniment for adults with intellectual disabilities.

  2. Nuclear arbitration: Interpreting non-proliferation agreements

    At the core of the nuclear non-proliferation regime lie international agreements. These agreements include, inter alia, the Nuclear Non-proliferation Treaty, nuclear co-operation agreements and nuclear export control agreements.1 States, however, do not always comply with their obligations under these agreements. In response, commentators have proposed various enforcement mechanisms to promote compliance. The inconvenient truth, however, is that states are generally unwilling to consent to enforcement mechanisms concerning issues as critical to national security as nuclear non-proliferation.3 This article suggests an alternative solution to the non-compliance problem: interpretation mechanisms. Although an interpretation mechanism does not have the teeth of an enforcement mechanism, it can induce compliance by providing an authoritative interpretation of a legal obligation. Interpretation mechanisms would help solve the non-compliance problem because, as this article shows, in many cases of alleged non-compliance with a non-proliferation agreement, the fundamental problem has been the lack of an authoritative interpretation of the agreement, not the lack of an enforcement mechanism. Specifically, this article proposes arbitration as the proper interpretation mechanism for non-proliferation agreements. It advocates the establishment of a 'Nuclear Arbitration Centre' as an independent branch of the International Atomic Energy Agency (IAEA), and recommends the gradual introduction of arbitration clauses into the texts of non-proliferation agreements. Section I begins with a discussion of international agreements in general and the importance of interpretation and enforcement mechanisms. Section II then discusses nuclear non-proliferation agreements and their lack of interpretation and enforcement mechanisms. Section III examines seven case studies of alleged non-compliance with non-proliferation agreements in order to show that the main problem in many cases

  3. Moderating Effect of Accompaniment on the Relationship Between Online Perceived Quality and Perceived Value

    Nozha Erragcha

    2012-06-01

    Full Text Available The aim of this research is to highlight the impact of the mediating effect of the perceived value on the influence of perceived quality of the purchase-site on the buying intention, Moreover; our goal is to study the moderating effect of accompaniment on the relationship between the perceived quality on perceived value web site. The data-gathering was carried out through an experimentation conducted an online-trading site for a laptop purchase. The findings reveal that the perceived quality partially influence the perceived value. The latter exerts a positive effect upon the buying intention. Accompaniment moderates the relationship between the perceived quality and the perceived value.

  4. Peculiarities of infectious diseases course accompanied by quinsy syndrome in children (data from children infectious hospital

    Ovchinnikova T.A.

    2011-03-01

    Full Text Available The research goal is to study morbidity dynamics for the period of 15 years and to determine clinical signs that accompany quinsy syndrome (diphtheria, infectious mononucleosis, scarlet fever, quinsy. Retrospective study analysis of annual reports and case-histories was carried out. 323 cases of infectious diseases accompanied by quinsy syndrome were examined. Clinical and epidemic signs of diseases were determined during the period of morbidity raise. The current clinical course of diseases was characterized in detail. The significant percentage of renal complications in case of pharyngonasal cavity lesion was shown

  5. 30 CFR 250.215 - What hydrogen sulfide (H2S) information must accompany the EP?

    2010-07-01

    ... methodologies outlined in 40 CFR part 68. ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What hydrogen sulfide (H2S) information must... Contents of Exploration Plans (ep) § 250.215 What hydrogen sulfide (H2S) information must accompany the...

  6. 76 FR 69738 - Revised 2011 Annual Telecommunications Reporting Worksheet (FCC Form 499-A) and Accompanying...

    2011-11-09

    ... COMMISSION Revised 2011 Annual Telecommunications Reporting Worksheet (FCC Form 499-A) and Accompanying... Competition Bureau released the revised Telecommunications Reporting Worksheet (FCC Form 499-A) and... Competition Bureau, Telecommunications Access Policy Division, at (202) 418-7400 or via the Internet...

  7. STUDY OF THE GAMMA EMISSION PROBABILITY ACCOMPANYING THE SPONTANEOUS FISSION OF CF-252

    VANDERPLOEG, H; LAURENS, CR; BACELAR, JCS; BUDA, A; GAARDHOJE, JJ; VANTHOF, G; KALANTARNAYESTANAKI, N; VANDERWOUDE, A; ZELAZNY, Z

    1994-01-01

    A study of the gamma emission accompanying the spontaneous fission process of Cf-252 has been performed. The photon emission probability between 3 and 70 MeV and its angular dependence with respect to the fission direction were measured. These measurements were performed as a function of the mass as

  8. Light-particle multiplicity accompanying projectile breakup at 20 MeV/A

    A large-solid-angle array has been used to determine the multiplicity of Z = 1 and Z = 2 particles accompanying projectile breakup into two fragments each with 5 ≤ Z ≤ 8. The charge balance obtained shows that projectile breakup is not associated with an unusual charge transfer to the target. 13 refs., 1 fig

  9. Clinical accompaniment: the critical care nursing students’ experiences in a private hospital

    N. Tsele

    2000-09-01

    Full Text Available The quality of clinical accompaniment of the student enrolled for the post-basic diploma in Medical and Surgical Nursing Science: Critical Care Nursing (General is an important dimension of the educational/learning programme. The clinical accompanist/mentor is responsible for ensuring the student’s compliance with the clinical outcomes of the programme in accordance with the requirements laid down by the Nursing Education Institution and the South African Nursing Council. The purpose of this study was to explore and describe the experiences of the students enrolled for a post-basic diploma in Medical and Surgical Nursing Science: Critical Care Nursing (General, in relation to the clinical accompaniment in a private hospital in Gauteng. An exploratory, descriptive and phenomenological research design was utilised and individual interviews were conducted with the ten students in the research hospital. A content analysis was conducted and the results revealed both positive and negative experiences by the students in the internal and external worlds. The recommendations include the formulation of standards for clinical accompaniment of students. the evaluation of the quality of clinical accompaniment of students and empowerment of the organisation, clinical accompanists/mentors and clinicians.

  10. Euclidean wormholes with Phantom field and Phantom field accompanied by perfect fluid

    Darabi, F.

    2010-01-01

    We study the classical Euclidean wormhole solutions for the gravitational systems with minimally coupled pure Phantom field and minimally coupled Phantom field accompanied by perfect fluid. It is shown that such solutions do exist and then the general forms of the Phantom field potential are obtained for which there are classical Euclidean wormhole solutions.

  11. 50 CFR 26.24 - Exception for entry when accompanied by refuge personnel.

    2010-10-01

    ... refuge personnel. 26.24 Section 26.24 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) THE NATIONAL WILDLIFE REFUGE SYSTEM PUBLIC ENTRY AND USE Public Entry § 26.24 Exception for entry when accompanied by refuge personnel. A permit is not required for...

  12. Analysis on the environmental problems accompanying the high-speed urbanization of small towns in China

    2002-01-01

    The environmental problems accompanying the rapid urbanization of many small towns like Keqiao, SHaoxing County, and their countermeasures are presented. To settle these problems rationally as well as effectively will not only conduce to boost the environmental quality and life quality of cities, but also help to promote the development of regional economy and the realization of sustainable development.

  13. Effects of Music Instruction with Bamboo Xylophone Accompaniment on Singing Achievement among Second-Grade Children

    Simeon, Jinky Jane C.; Ku, Agnes Chun Moi

    2015-01-01

    The aim of this research is to determine the effect of music instruction with bamboo xylophone as harmonic accompaniment on the singing achievement of second-grade children. Eighty children (N = 80) from four randomly selected classes in two different public schools in the city of Kota Kinabalu participated in this study and they were assigned to…

  14. A Qualitative Investigation into the Characteristics and Effects of Music Accompanying Exercise

    Priest, David-Lee; Karageorghis, Costas I.

    2008-01-01

    The purpose of the present study was to identify the characteristics of music used to accompany physical exercise and investigate the effects of such music using a qualitative approach. This work underpins the further development of a theoretical structure that is still relatively new. Semi-structured interviews were conducted with a sample of…

  15. Investigation of the Majoron-accompanied double-beta decay mode of [sup 76]Ge

    Beck, M.; Bensch, F.; Bockholt, J.; Heusser, G.; Hirsch, M.; Klapdor-Kleingrothaus, H.V.; Maier, B.; Petry, F.; Piepke, A.; Strecker, H.; Voellinger, M.; Zuber, K.; Balysh, A.; Belyaev, S.T.; Demehin, A.; Gurov, A.; Kondratenko, I.; Lebedev, V.I.; Mueller, A. (Max-Planck-Institut fuer Kernphysik, P.O. Box 103980, 6900 Heidelberg (Germany) Kurchatov Institute, Moscow (Russian Federation) LNGS, Istituto Nazionale di Fisica Nucleare, Gran Sasso, Assergi (Italy))

    1993-05-10

    We have examined the double-beta decay mode accompanied by Majoron emission. After 223 days of measurement corresponding to about 615 kg d or 19.3 mol yr of exposure we find a lower half-life limit for this decay channel of [ital T][sub 1/2][sup 0[nu][chi

  16. 41 CFR 101-25.110-3 - Tires accompanying new motor vehicles.

    2010-07-01

    ... motor vehicles. 101-25.110-3 Section 101-25.110-3 Public Contracts and Property Management Federal...-GENERAL 25.1-General Policies § 101-25.110-3 Tires accompanying new motor vehicles. The tire identifications and recordkeeping regulations issued by the Department of Transportation require each...

  17. 75 FR 61489 - Renewal of Declaration Regarding Emergency Use of Doxycycline Hyclate Tablets Accompanied by...

    2010-10-05

    ... provided at 73 FR 58242 (October 6, 2008). Dated: September 24, 2010. Kathleen Sebelius, Secretary. BILLING... HUMAN SERVICES Office of the Secretary Renewal of Declaration Regarding Emergency Use of Doxycycline... use of doxycycline hyclate tablets accompanied by emergency use information subject to the terms...

  18. Director`s series on proliferation

    Bailey, K.C. [ed.

    1993-09-07

    Two essays are included in this booklet. Their titles are ``The Dynamics of the NPT Extension Decision`` and ``North Korea`s Nuclear Gambit.`` The first paper discusses the conference to be held in 1995 to review the Nuclear Non-Proliferation Treaty (NPT) which will decide whether the treaty shall continue in force indefinitely, or shall be extended for an additional fixed period or periods. Topics relevant to this discussion are: Arms control issues, the nuclear test ban, the limited test ban treaty, the French nuclear testing moratorium, former Soviet nuclear weapons, Iraq, North Korea, nuclear-weapon-free zones, security, controls on nuclear weapon materials, peaceful uses of nuclear energy, safeguards, politics, and organizational and procedural issues. The second paper examines short, medium, and long term issues entailed in Korea`s nuclear proliferation. Topics considered include: Korean unification, North Korean politics, the nuclear issue as leverage, and the Nuclear Non- Proliferation Treaty.

  19. Improving nuclear economics without increasing proliferation risk

    The postponement of recycle systems is an insurance against the premature plunge into widespread commercial use of plutonium, with its potential for increasing proliferation risks. We can afford to postpone the deployment process with little penalty by improving light water reactors in once-through mode (LWROT). This would make nuclear power cheaper without increasing proliferation risk. The deferral is desirable because very valuable information about the feasibility, competitiveness, and resistivity to proliferation of technologies such as advance converters, the once-through fast reactor, and the fusion-fission hybrid reactor will be learned in the next 10 years. There is a distinct possibility that, as far as nuclear energy is concerned, we can rely on LWR(OT)s with evolutionary improvements far into the future. 29 references, 2 tables

  20. Israel's position on non-proliferation

    Israel maintained that the complex international system and worldwide political tension created a situation in which comprehensive plans of disarmament could not produce any positive result. The deadlock in the field of general and complete disarmament has brought Israel to the realization that one possible way to alleviate the stalemate could be progress by stages through partial measures of disarmament. Israel's position on non-proliferation indicates that the establishment of a nuclear-weapon-free-zone (NWFZ), as it relates to the Middle-East, could serve as a credible alternative to the unilateral adherence to the Non-Proliferation of Nuclear Weapon (NPT) and an effective measure of non-proliferation in the region. (Author)

  1. Proliferation resistance assessment of thermal recycle systems

    This paper examines the major proliferation aspects of thermal recycle systems and the extent to which technical or institutional measures could increase the difficulty or detectability of misuse of the system by would-be proliferators. It does this by examining the various activities necessary to acquire weapons-usable material using a series of assessment factors; resources required, time required, detectability. It is concluded that resistance to proliferation could be improved substantially by collecting reprocessing, conversion and fuel fabrication plants under multi national control and instituting new measures to protect fresh MOX fuel. Resistance to theft at sub-national level could be improved by co-location of sensitive facilities high levels of physical protection at plants and during transportation and possibly by adding a radiation barrier to MOX prior to shipment

  2. Does programmed CTL proliferation optimize virus control?

    Wodarz, Dominik; Thomsen, Allan Randrup

    2005-01-01

    CD8 T-cell or cytotoxic T-lymphocyte responses develop through an antigen-independent proliferation and differentiation program. This is in contrast to the previous thinking, which was that continuous antigenic stimulation was required. This Opinion discusses why nature has chosen the proliferation...... program and how it compares to continuous stimulation. Although the two mechanisms should not lead to significantly different dynamics during chronic infection, they do make a difference in acute infection. We argue that programmed proliferation is better at clearance, whereas continuous stimulation is...... better at limiting acute symptoms. The 7-10 programmed cell divisions observed in vivo might be an optimization of this trade-off. We also discuss the conditions under which the program does or does not require CD4 T-cell help for clearance....

  3. Cell proliferation and differentiation in chemical leukemogenesis

    Irons, R. D.; Stillman, W. S.; Clarkson, T. W. (Principal Investigator)

    1993-01-01

    In tissues such as bone marrow with normally high rates of cell division, proliferation is tightly coordinated with cell differentiation. Survival, proliferation and differentiation of early hematopoietic progenitor cells depend on the growth factors, interleukin 3 (IL-3) and/or granulocyte-macrophage colony stimulating factor (GM-CSF) and their synergism with other cytokines. We provide evidence that a characteristic shared by a diverse group of compounds with demonstrated leukemogenic potential is the ability to act synergistically with GM-CSF. This results in an increase in recruitment of a resting population of hematopoietic progenitor cells normally unresponsive to the cytokine and a twofold increase in the size of the proliferating cell population normally regarded to be at risk of transformation in leukemogenesis. These findings support the possibility that transient alterations in hematopoietic progenitor cell differentiation may be an important factor in the early stages of development of leukemia secondary to chemical or drug exposure.

  4. Factors influencing the proliferation of nuclear weapons

    Although predicting that there will be more nuclear weapons in the future is safe enough, predicting how many more countries over what period of time, which ones under what circumstances, and how much more of what kind of nuclear-weapons capability for each is more difficult. Predicting what effect more will have is a related question that is even more difficult. This chapter identifies some of the factors that will determine the answers: including the supply policies of nuclear-exporting counties, the international nuclear-fuel cycle, superpower security policies, the health of the non-proliferation regime, cataclysmic events and international reaction, and preventive and preemptive strikes. The author concludes that more proliferation will only be better if we accept the greater probability of catastrophic wars in exchange for less frequent conventional conflicts, but he tempers this with the observation that the policies of governments can influence the course of proliferation

  5. Director`s series on proliferation

    Bailey, K.C.; Price, M.E. [eds.

    1994-12-27

    The Director`s Series on Proliferation is an occasional publication of essays on the topics of nuclear, chemical, biological, and missile proliferation. The seven papers presented in this issue cover the following topics: Should the Treaty on the Nonproliferation of Nuclear Weapons (NPT) be amended?; NPT extension - Legal and procedural issues; An Indonesian view of NPT review conference issues; The treaty of Tlatelolco and the NPT - Tools for peace and development; Perspectives on cut-off, weapons dismantlement, and security assurances; Belarus and NPT challenges; A perspective on the chemical weapons convention - Lessons learned from the preparatory commission.

  6. Proliferation risks; Proliferatierisico's

    Carchon, R

    1998-09-01

    The report gives an overview of different aspects related to safeguards of fissile materials. Existing treaties including the Non-Proliferation Treaty, and the Tlatelolco and the Rarotonga Treaties are discussed. An overview of safeguards systems for the control of fissile materials as well as the role of various authorities is given. An overall overview of proliferation risks, the physical protection of fissile materials and the trade in fissile materials is given. Finally, the status in problem countries and de facto nuclear weapon states is discussed.

  7. Supervised Semantic Classification for Nuclear Proliferation Monitoring

    Vatsavai, Raju [ORNL; Cheriyadat, Anil M [ORNL; Gleason, Shaun Scott [ORNL

    2010-01-01

    Existing feature extraction and classification approaches are not suitable for monitoring proliferation activity using high-resolution multi-temporal remote sensing imagery. In this paper we present a supervised semantic labeling framework based on the Latent Dirichlet Allocation method. This framework is used to analyze over 120 images collected under different spatial and temporal settings over the globe representing three major semantic categories: airports, nuclear, and coal power plants. Initial experimental results show a reasonable discrimination of these three categories even though coal and nuclear images share highly common and overlapping objects. This research also identified several research challenges associated with nuclear proliferation monitoring using high resolution remote sensing images.

  8. Invasion and Proliferation in Malignant Cells

    Svensson Månsson, Sofie

    2006-01-01

    Two key events in the oncogenic process of tumor cells are to acquire uncontrolled proliferation and invasive properties. This allows the tumor to grow and invade beyond the tissue from which the tumor cells originate. We here specifically studied p16 and ERK1/2 with special focus on and the relation to proliferation and invasion in non-melanoma skin cancer and in breast cancer. In a model system of basal cell carcinoma, we observed that tumor cells changed phenotype from a highly prol...

  9. Proliferation resistance criteria for fissile material disposition

    The 1994 National Academy of Sciences study open-quotes Management and Disposition of Excess Weapons Plutoniumclose quotes defined options for reducing the national and international proliferation risks of materials declared excess to the nuclear weapons program. This report proposes criteria for assessing the proliferation resistance of these options. The criteria are general, encompassing all stages of the disposition process from storage through intermediate processing to final disposition including the facilities, processing technologies and materials, the level of safeguards for these materials, and the national/subnational threat to the materials

  10. Molecular Crowding Defines a Common Origin for the Warburg Effect in Proliferating Cells and the Lactate Threshold in Muscle Physiology

    Vazquez, Alexei; Oltvai, Zoltán N.

    2011-01-01

    Aerobic glycolysis is a seemingly wasteful mode of ATP production that is seen both in rapidly proliferating mammalian cells and highly active contracting muscles, but whether there is a common origin for its presence in these widely different systems is unknown. To study this issue, here we develop a model of human central metabolism that incorporates a solvent capacity constraint of metabolic enzymes and mitochondria, accounting for their occupied volume densities, while assuming glucose and/or fatty acid utilization. The model demonstrates that activation of aerobic glycolysis is favored above a threshold metabolic rate in both rapidly proliferating cells and heavily contracting muscles, because it provides higher ATP yield per volume density than mitochondrial oxidative phosphorylation. In the case of muscle physiology, the model also predicts that before the lactate switch, fatty acid oxidation increases, reaches a maximum, and then decreases to zero with concomitant increase in glucose utilization, in agreement with the empirical evidence. These results are further corroborated by a larger scale model, including biosynthesis of major cell biomass components. The larger scale model also predicts that in proliferating cells the lactate switch is accompanied by activation of glutaminolysis, another distinctive feature of the Warburg effect. In conclusion, intracellular molecular crowding is a fundamental constraint for cell metabolism in both rapidly proliferating- and non-proliferating cells with high metabolic demand. Addition of this constraint to metabolic flux balance models can explain several observations of mammalian cell metabolism under steady state conditions. PMID:21559344

  11. Molecular crowding defines a common origin for the Warburg effect in proliferating cells and the lactate threshold in muscle physiology.

    Alexei Vazquez

    Full Text Available Aerobic glycolysis is a seemingly wasteful mode of ATP production that is seen both in rapidly proliferating mammalian cells and highly active contracting muscles, but whether there is a common origin for its presence in these widely different systems is unknown. To study this issue, here we develop a model of human central metabolism that incorporates a solvent capacity constraint of metabolic enzymes and mitochondria, accounting for their occupied volume densities, while assuming glucose and/or fatty acid utilization. The model demonstrates that activation of aerobic glycolysis is favored above a threshold metabolic rate in both rapidly proliferating cells and heavily contracting muscles, because it provides higher ATP yield per volume density than mitochondrial oxidative phosphorylation. In the case of muscle physiology, the model also predicts that before the lactate switch, fatty acid oxidation increases, reaches a maximum, and then decreases to zero with concomitant increase in glucose utilization, in agreement with the empirical evidence. These results are further corroborated by a larger scale model, including biosynthesis of major cell biomass components. The larger scale model also predicts that in proliferating cells the lactate switch is accompanied by activation of glutaminolysis, another distinctive feature of the Warburg effect. In conclusion, intracellular molecular crowding is a fundamental constraint for cell metabolism in both rapidly proliferating- and non-proliferating cells with high metabolic demand. Addition of this constraint to metabolic flux balance models can explain several observations of mammalian cell metabolism under steady state conditions.

  12. Proliferation Resistant Fuel for Pebble Bed Modular Reactors

    Proliferation of nuclear weapons produced with power reactors plutonium has always been amajor problem of the nuclear energy industry. This includes the PebbleBed Modular Reactor(PBMR), which is a specific design of a GenIV High-Temperature Reactor (HTR), mainly due to its online refueling feature, which may be misused for the production of weapons gradeplutonium. A promising approach toward preventing the proliferation of power reactorplutoniumis to denaturate the plutonium by increasing the ratio of 238Pu to total Pu in the spentfuel(1). The 238Pu isotope is characterized by a high heat rate (approximately 567 W/kg) due to thealpha decay of the 238Pu with half-life of 87.74 yr, in addition to its high spontaneous fissionneutron emission, which is higher than that of 240Pu. Thus, the presence of 238Pu in Pu considerably complicates the design and construction of nuclear weapons based on Pu, owing tothese characteristics of 238Pu. Recent papers(2,3) show that a Pu mixture is proliferation resistant given that the weight ratio of 238Pu to Pu is larger than 6%. In this paper we have studied afeasible technique for ensuring that the 238Pu to Pu ratio, in the Pu produced in PBMR, is larger than 6% during the entire fuel cycle. Contamination of the spent fuel with 238Pu may be achieved by doping the nuclear fuel witheither 241Am or 237Np(4-13). The 238Pu isotope is obtained from both 241Am and 237Np by a neutron-capture reaction and the subsequent decay of the reaction products(13).The 237Np isotopeis by itself a potential weapons grade material. However, its large critical mass of 57±4 kg(14) andthe difficulty of extracting it from irradiated fuel elements make it impractical for weapons purposes. On the other hand, the critical mass of 241Am is smaller, i.e. 34 to 45 kg. However, withdecay heat production of 114W/kg, the critical mass becomes a heat source of 3.9 to 5.1 KW,which makes 241Am unsuitable for weapons applications(3). As a result, it is a non-proliferating

  13. Non-proliferation and nuclear disarmament

    Fissionable materials are common to all nuclear weapons and controls on the production, storage, processing and use of fissionable materials provides one means to address non-proliferation and disarmament. In this article, the relevance of such controls is examined and the current situation and future prospects are assessed. (authors)

  14. Nuclear Proliferation and Authority in World Politics

    Brian Frederking

    2009-11-01

    Full Text Available We apply the “security-hierarchy paradox” to nuclear proliferation. Global security requires a certain amount of hierarchy. A world in which no nuclear proliferation rules exist to constrain states, for example, would not be secure. Global security requires legitimate and authoritative rules, which we define as rules that are mutually negotiated, binding to all andwhich provide a stable social order. Too much hierarchy, however, amounts to coercion andundermines global security. Rules that are not mutually negotiated, binding to all or do not provide a stable social order are not authoritative. We argue that North Korea and Iran haveattempted to build nuclear weapons because they interpret the proliferation rules to lack authority. The coercive U.S. approaches to enforcing proliferation rules – including diplomatic isolation, preemption, and regime change – have undermined the legitimacy of those rules. When the U.S. pursues less hierarchical policies, as it has recently toward North Korea, the ensuing negotiations have facilitated progress toward an agreement. When theU.S. pursues a consistently hierarchical approach, as it has toward Iran, no progress is made. Our analysis suggests that it is worth attempting a less hierarchical approach toward Iran and encourage it to accept a deal similar to the one negotiated with North Korea.

  15. Canada's nuclear non-proliferation policy

    Canada's non-proliferation and safeguards policy has two objectives: 1) to promote the emergence of a more effective and comprehensive international non-proliferation regime; and 2) to assure the Canadian people and the international community that Canadian nuclear exports will not be used for any nuclear explosive purpose. By emphasizing the key role of the NPT, by promoting reliance upon and improvements in the IAEA safeguards system, by treating nuclear weapon and non-nuclear weapon states alike regarding Canadian nuclear exports, by working for new approaches covering the sensitive phases (e.g. reprocessing) of the nuclear fuel cycle, Canada's policy promotes attainment of the first objective. The latter objective is served through the network of bilateral nuclear agreements that Canada has put into place with its nuclear partners. Those agreements provide assurance that Canada's nuclear exports are used solely for legitimate, peaceful, nuclear energy production purposes. At the same time, Canada, having formulated its non-proliferation and safeguards policy during the period 1945 to 1980, has recognized that it has gone as far as it can on its own in this field and that from this point on any further changes should be made on the basis of international agreement. The Canadian objective in post-INFCE forums such as the Committee on Assurances of Supply is to exert Canada's best efforts to persuade the international community to devise a more effective and comprehensive international non-proliferation regime into which Canada and other suppliers might subsume their national requirements

  16. Effect of chloroquine on human lymphocyte proliferation

    Bygbjerg, Ib Christian; Flachs, H

    1986-01-01

    The effect of chloroquine on human blood mononuclear cells was studied. High concentrations of chloroquine in vitro profoundly suppressed the proliferation of mitogen- and antigen-stimulated cells, as indicated by decreased 14C-thymidine incorporation. Lower concentrations of chloroquine increase...

  17. Liposuction: Extracellular Fat Removal Promotes Proliferation.

    Egnatchik, Robert A; DeBerardinis, Ralph J

    2016-04-21

    In this issue of Cell Chemical Biology, Yao et al. (2016) investigate the makeup of lipid membranes of both cancer and non-transformed cells to reveal that doubling cells preferentially use exogenous fatty acids over de novo synthesis to proliferate. PMID:27105278

  18. Developing a non-proliferation culture

    Mr. J Joly, the President of the European Safeguards Research and Development Association (ESARDA), said that ESARDA has been actively involved in developing and promoting safeguards for more than 35 years and he is pleased to cooperate with the IAEA and the Institute of Nuclear Materials Management in the organization of this symposium. He proposes the development of a 'non-proliferation culture' as a key principle and defines a non-proliferation culture as the characteristics and attitudes of organizations and individuals that: (i) relate to the protection against the diversion and theft of nuclear material and the provision of information about research and development related to the nuclear fuel cycle; and (ii) receive the attention warranted by their significance. According to him the non-proliferation culture has three universal features: (i) policy commitments at the State level; (ii) the organizational framework and commitments; and (iii) the attitudes and behaviour of the staff members of these organizations. These aspects should be considered as a whole, and they should demonstrate transparency with respect to States' exclusively peaceful nuclear activities and contribute to establishing confidence among States and regions of the world. He concludes that a synergy should be developed between the cultures of nuclear non-proliferation, security and safety. Each of these cultures represents a key principle and, as such, can help to establish confidence among States and regions of the world. To the public, these cultures should represent professionalism, competence and responsibility by all parties involved

  19. Strengthening the foundations of proliferation assessment tools.

    Rexroth, Paul E.; Saltiel, David H.; Rochau, Gary Eugene; Cleary, Virginia D.; Ng, Selena (AREVA NC, Paris, France); Greneche, Dominique (AREVA NC, Paris, France); Giannangeli, Don (Texas A& M University, College Station, TX); Charlton, William S. (Texas A& M University, College Station, TX); Ford, David (Texas A& M University, College Station, TX)

    2007-09-01

    Robust and reliable quantitative proliferation assessment tools have the potential to contribute significantly to a strengthened nonproliferation regime and to the future deployment of nuclear fuel cycle technologies. Efforts to quantify proliferation resistance have thus far met with limited success due to the inherent subjectivity of the problem and interdependencies between attributes that lead to proliferation resistance. We suggest that these limitations flow substantially from weaknesses in the foundations of existing methodologies--the initial data inputs. In most existing methodologies, little consideration has been given to the utilization of varying types of inputs--particularly the mixing of subjective and objective data--or to identifying, understanding, and untangling relationships and dependencies between inputs. To address these concerns, a model set of inputs is suggested that could potentially be employed in multiple approaches. We present an input classification scheme and the initial results of testing for relationships between these inputs. We will discuss how classifying and testing the relationship between these inputs can help strengthen tools to assess the proliferation risk of nuclear fuel cycle processes, systems, and facilities.

  20. Limiting Future Proliferation and Security Risks

    A major new technical tool for evaluation of proliferation and security risks has emerged over the past decade as part the activities of the Generation IV International Forum. The tool has been developed by a consensus group from participating countries and organizations and is termed the Proliferation Resistance and Physical Protection (PR and PP) Evaluation Methodology. The methodology defines a set of challenges, analyzes system response to these challenges, and assesses outcomes. The challenges are the threats posed by potential actors (proliferant states or sub-national adversaries). It is of paramount importance in an evaluation to establish the objectives, capabilities, resources, and strategies of the adversary as well as the design and protection contexts. Technical and institutional characteristics are both used to evaluate the response of the system and to determine its resistance against proliferation threats and robustness against sabotage and terrorism threats. The outcomes of the system response are expressed in terms of a set of measures, which thereby define the PR and PP characteristics of the system. This paper summarizes results of applications of the methodology to nuclear energy systems including reprocessing facilities and large and small modular reactors. The use of the methodology in the design phase a facility will be discussed as it applies to future safeguards concepts.

  1. Non-proliferation and the nuclear industry

    There has presumably never been any doubt about the nuclear industry's fundamental support for international efforts to halt the horizontal spread of nuclear weapons. The statement of concern issued by the Uranium Institute before the Third Review Conference of the Non-Proliferation Treaty (NPT) in 1985 was striking testimony to that effect. The imminence of the Fourth Review Conference is nevertheless an occasion to reflect again on some of the reasons why the industry has no prudent choice but to involve itself in non-proliferation questions. The reasons stretch along a spectrum. At one end are general and powerful arguments for the nuclear industry to share with other industries in the broad expression of social responsibility. At the other end are narrowly specific connections between non-proliferation regulations on the processing, movement, storage and use of fissionable and other nuclear materials and the practical conduct of operations in and around the civil nuclear fuel cycle. In between the two come considerations not of how non-proliferation issues directly cause specific commercial effects but of how they help, indirectly and in the longer term, to shape the social environment and political climate in which nuclear power will flourish or wither. (author)

  2. Nuclear proliferation, a legacy of history

    The author proposes an historic perspective on nuclear proliferation. He describes how, at the beginning of the twentieth century, nuclear science was at first mobilising scientists rather than states or countries. At that time, knowledge was shared among European countries and knowledge spread through scientific publications or academic exchanges. Moreover, during the 1930's, some of these scientists decided to move to the USA. Thus, Germany lost a lot of its scientific potential. Proliferation occurred quite naturally from Los Alamos to Great-Britain, then by spies to USSR. By the end of the war, each winner country possessed some abilities. Then, the author describes how China managed to acquire nuclear technology with the support of cooperation with USSR, and based on a group of scientists and physicists who had worked with European physicists like Bohr or Joliot-Curie. The author then comments the other proliferation mechanisms despite the existence of or in parallel with the NPT and various resolutions adopted by the UNO to correct some shortcomings of the Treaty. He outlines the risk of occurrence of a crisis for the non proliferation regime as countries who signed the NPT may develop a hidden military nuclear programme (like Iran for example). The author finally outlines the role France can play within the NPT

  3. Nuclear Society and non-proliferation problems

    In the USSR Nuclear Society in 1991 the special working group on the problems of nuclear weapons non-proliferation and nuclear materials control, uniting the experts of different types (nuclear physicists, lawyers, teachers), was created. This group became the mechanism of the practical Nuclear Society activity realization in this sphere. Three milestones of the innovative activity can be specified. First Milestone. In January 1992 the Central Nuclear Society Board (of the International Public Nuclear Society Association) published a special appeal to the First Leaders of all countries - former USSR republics. This address paid a special attention to the unity of the USSR power-industrial complex, and numerous problems arisen while separating this complex, including nuclear weapons non-proliferation problems, were indicated as well. Second Milestone. In 1992 and 1993 the Nuclear Society experts issued two selection 'Nuclear Non-proliferation and Control Problems' including reviewing basic papers. In addition, materials on non-proliferation and control are published regularly in the organs. Third Milestone.In 1993 - 1997 some special scientific and technical events (conferences, workshops, meetings) allowing to analyze the joint international projects and contracts outcomes, and establish new contacts between the specialists of NIS, Baltic states and others, have been hold

  4. Proliferation: does the peaceful use of nuclear energy have to lead to proliferation of nuclear weapons

    The question of whether the proliferation of nuclear weapons is promoted by an increasing use of peaceful nuclear energy can be answered with a well-founded no. Even a regional renouncing of the peaceful use of nuclear energy would not reduce the worldwide problem of nuclear weapons' proliferation. Therefore, joint efforts must be aimed at promoting trust between peoples in the nuclear sphere and the political reasons for the proliferation of nuclear weapons must be reduced in order also to promote international harmony

  5. Nuclear non proliferation and disarmament; Non-proliferation nucleaire et desarmement

    NONE

    2000-07-01

    In the framework of the publication of a document on the ''weapons mastership, disarmament and non proliferation: the french action'', by the ministry of Foreign Affairs and the ministry of Defense, the French Documentation organization presents a whole document. This document describes and details the following topics: the conference on the treaty of non proliferation of nuclear weapons, the France, Usa and Non Governmental Organizations position, the threats of the proliferation, the french actions towards the disarmament, the disarmament in the world, a chronology and some bibliographic resources. (A.L.B.)

  6. [Recurrence of thymoma accompanied with hypogammaglobulinemia 20 years after surgery: a case report].

    Naniwa, Taio; Kakihara, Hidetoshi; Zen-nami, Shuji; Tomita, Hiroshi; Sugiura, Yoshiki; Yoshinouchi, Takeo; Sato, Shigeki; Ueda, Ryuzo

    2002-03-01

    We reported a case of recurrence of localized thymoma accompanied with hypogammaglobulinemia (Good's syndrome) 20 years after surgery. A 74-year-old man was admitted to this hospital because of mediastinal tumor and chronic pulmonary infection. He had been thymectomised at the age of 55 because of spindle cell thymoma. After that, he had been asymptomatic until January 1997, when he began to have a recurrent productive cough, and low-grade fever. Laboratory findings revealed hypogammaglobulinemia. Percutaneous needle biopsy of the mediastinal tumor revealed spindle cell thymoma. Therefore, hypogammaglobulinemia with thymoma (Good's syndrome) accompanied with a chronic lower respiratory tract infection was diagnosed. Immunologic studies revealed a marked decrease of CD 20 positive cells and decreased lymphocyte activation under the stimuli of phytohemagglutinin and concanavalin A. The thymoma was resected in Dec 1997, but the serum immunoglobulin showed no increase at al. PMID:11974900

  7. Photothermal and accompanied phenomena of selective nanophotothermolysis with gold nanoparticles and laser pulses

    In medical applications of laser and nanotechnology to diagnosis and treat cancer or microorganisms, understanding of lased-induced photothermal (PT) and accompanied phenomena around nanoparticles are crucial for optimization and bringing this promising technology to bedside. We analyzed the main PT-based effects in and around gold nanoparticles under action of short (nano-, pico-, and femtosecond) laser pulses with focus on photoacoustic effects due to the thermal expansion of nanoparticles and liquid around them, thermal protein denaturation, explosive liquid vaporization, melting and evaporation of nanoparticle, optical breakdown initiated by nanoparticles and accompanied to shock waves and explosion (fragmentation) of gold nanoparticles. Characteristic parameters for these processes such as the temperature and pressures levels, and laser intensity thresholds among others are summarized to provide basis for comparison of different mechanisms of selective nanophotothermolysis and diagnostics of different targets (e.g., cancer cells, bacteria, viruses)

  8. Triple Peripheral Nerve Injury Accompanying to Traumatic Brain Injury: A Case Report

    Ižlknur Can

    2014-02-01

    Full Text Available Secondary injuries especially extremity fractures may be seen concurrently with traumatic brain injury (TBI. Peripheral nerve damages may accompany to these fractures and may be missed out, especially in acute stage. In this case report; damage of radial, ulnar and median nerves which was developed secondarily to distal humerus fracture that could not be detected in acute stage, in a patient who had motor vehicle accident (MVA. 29-year-old male patient was admitted with weakness in the right upper extremity. 9 months ago, he had traumatic brain injury because of MVA, and fracture of distal humerus was detected in follow-ups. Upon the suspect of the peripheral nerve injury, the diagnosis was confirmed with ENMG. The patient responded well to the rehabilitation program treatment. In a TBI patient, it must be kept in mind that there might be a secondary trauma and therefore peripheral nerve lesions may accompany to TBI.

  9. Prevalence of open angle glaucoma in accompanying first degree relatives of patients with glaucoma

    Franciele Vegini

    2008-01-01

    Full Text Available OBJECTIVE: The aim of this study was to determine the prevalence of open angle glaucoma in first-degree relatives accompanying POAG patients during routine examination in a reference hospital. METHOD: First-degree relatives of primary open angle glaucoma patients who accompanied their relatives to the glaucoma service of a reference hospital were screened for glaucoma. RESULTS: One-hundred and one first-degree relatives were examined, of which 56.4% had never had their intraocular pressure measured. 10.9% had previously been diagnosed with glaucoma, and 5.9% were newly diagnosed during this study. CONCLUSIONS: The eye examination of first-degree relatives identified a significant percentage of individuals with glaucoma. Despite being first-degree relatives of glaucoma patients, 56.4% of the companions had never had their eye pressure measured, demonstrating a lack of awareness about this disease.

  10. Size distributions of air showers accompanied with high energy gamma ray bundles observed at Mt. Chacaltaya

    Matano, T.; Machida, M.; Tsuchima, I.; Kawasumi, N.; Honda, K.; Hashimoto, K.; Martinic, N.; Zapata, J.; Navia, C. E.; Aquirre, C.

    1985-01-01

    Size distributions of air showers accompanied with bundle of high energy gamma rays and/or large size bursts under emulsion chambers, to study the composition of primary cosmic rays and also characteristics of high energy nuclear interaction. Air showers initiated by particles with a large cross section of interaction may develop from narrow region of the atmosphere near the top. Starting levels of air showers by particles with smaller cross section fluctuate in wider region of the atmosphere. Air showers of extremely small size accompanied with bundle of gamma rays may be ones initiated by protons at lower level after penetrating deep atmosphere without interaction. It is determined that the relative size distribution according to the total energy of bundle of gamma rays and the total burst size observed under 15 cm lead absorber.

  11. Grain growth and thermal stability accompanying recrystallization in undercooled Ni-3at.%Sn alloy

    Chen, Z., E-mail: chenzheng1218@163.com [School of Material Science and Engineering, China University of Mining and Technology, Xuzhou, Jiangsu 221116 (China); State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xi' an, Shaanxi 710016 (China); Chen, Q.; Shen, C.J. [School of Material Science and Engineering, China University of Mining and Technology, Xuzhou, Jiangsu 221116 (China); Liu, F. [State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xi' an, Shaanxi 710016 (China)

    2015-10-15

    The grain growth and thermal stability after recrystallization in as-solidified highly undercooled Ni-3at.%Sn alloy melt were investigated. As for undercooled Ni-3at.%Sn alloy, a transition from dendritic to granular crystals occurred when ΔT≥ΔT{sup *}, which was induced by the plastic deformation of dendrites and subsequent recrystallization. On this basis, the subsequent grain growth and solute segregation accompanying recalescence were calculated by a recently proposed thermo-kinetic model, which showed close agreement with the experimental results. It is concluded that the grain growth process was interrelated to recalescence, solute trapping and solute segregation of Sn atoms captured by solute trapping, which was responsible for the reduction of grain boundary energy and improvement of thermal stability. - Highlights: • A transition from dendritic to granular crystals occurred when ΔT≥ΔT{sup *}. • The grain growth accompanying recalescence was calculated. • A close agreement with the experimental results was shown.

  12. Pitch-informed solo and accompaniment separation towards its use in music education applications

    Cano, Estefanía; Schuller, Gerald; Dittmar, Christian

    2014-12-01

    We present a system for the automatic separation of solo instruments and music accompaniment in polyphonic music recordings. Our approach is based on a pitch detection front-end and a tone-based spectral estimation. We assess the plausibility of using sound separation technologies to create practice material in a music education context. To better understand the sound separation quality requirements in music education, a listening test was conducted to determine the most perceptually relevant signal distortions that need to be improved. Results from the listening test show that solo and accompaniment tracks pose different quality requirements and should be optimized differently. We propose and evaluate algorithm modifications to better understand their effects on objective perceptual quality measures. Finally, we outline possible ways of optimizing our separation approach to better suit the requirements of music education applications.

  13. Pentalogy of Cantrell accompanied by scoliosis and pes equinovarus deformity at 12 weeks gestation.

    Pekin, A Tazegül; Kerimoğlu, O Seçilmiş; Yilmaz, S A; Kebapcilar, A G; Bakbak, B Gencoğlu; Celik, C

    2015-01-01

    Cantrell's pentalogy (CP) is a rare syndrome characterized by defects in the lower sternum with ectopia cordis, anterior diaphragm defects, midline supraumbilical abdominal wall defects, defects in the diaphragmatic pericardium, and congenital heart disease. The authors report a 12-weeks gestation with multiple fetal anomalies suggesting the diagnosis of CP (a large thoraco-abdominal defect with herniating liver and bowel, heart deviated anteriorly with concomitant ventricular septal defect), and the 'S' shaped fetal spine due to increased lumbar lordosis and scoliosis with accompanying pes equinovarus deformity. Chorionic villus sampling was performed due to increased nuchal translucency (3.7 mm). The fetal karotype was found to be 47, XX,+21 (trisomy 21). In the literature, three scoliosis cases have been reported accompanying the CP along with multiple anomalies and one concomitant pes equinovarus deformity has been reported previously. PMID:26152022

  14. In Vitro Endothelial Cell Proliferation Assay Reveals Distinct Levels of Proangiogenic Cytokines Characterizing Sera of Healthy Subjects and of Patients with Heart Failure

    Rebecca Voltan

    2014-01-01

    Full Text Available Although myocardial angiogenesis is thought to play an important role in heart failure (HF, the involvement of circulating proinflammatory and proangiogenic cytokines in the pathogenesis and/or prognosis of HF has not been deeply investigated. By using a highly standardized proliferation assay with human endothelial cells, we first demonstrated that sera from older (mean age 52±7.6 years; n=46 healthy donors promoted endothelial cell proliferation to a significantly higher extent compared to sera obtained from younger healthy donors (mean age 29±8.6 years; n=20. The promotion of endothelial cell proliferation was accompanied by high serum levels of several proangiogenic cytokines. When we assessed endothelial cell proliferation in response to HF patients’ sera, we observed that a subset of sera (n=11 promoted cell proliferation to a significantly lesser extent compared to the majority of sera (n=18. Also, in this case, the difference between the patient groups in the ability to induce endothelial cell proliferation correlated to significant (P<0.05 differences in serum proangiogenic cytokine levels. Unexpectedly, HF patients associated to the highest endothelial proliferation index showed the worst prognosis as evaluated in terms of subsequent cardiovascular events in the follow-up, suggesting that high levels of circulating proangiogenic cytokines might be related to a worse prognosis.

  15. HIFI: a computer code for projectile fragmentation accompanied by incomplete fusion

    A brief summary of a model proposed to describe projectile fragmentation accompanied by incomplete fusion and the instructions for the use of the computer code HIFI are given. The code HIFI calculates single inclusive spectra, coincident spectra and excitation functions resulting from particle-induced reactions. It is a multipurpose program which can calculate any type of coincident spectra as long as the reaction is assumed to take place in two steps

  16. Brain reward deficits accompany withdrawal (hangover) from acute ethanol in rats

    Schulteis, Gery; Liu, Jian

    2006-01-01

    Withdrawal from an acute bolus injection of ethanol produces affective or emotional signs that include anxiogenic-like behavior (Gauvin et al., 1992) and conditioned place aversion (Morse et al., 2000). The current study assessed whether brain reward deficits that accompany withdrawal from chronic ethanol dependence (Schulteis et al., 1995) are also observed upon withdrawal from acute intoxication. Rats were implanted with stimulating electrodes aimed at the medial forebrain bundle in the lat...

  17. The internal bremsstrahlung accompanying the β-dacay from 185W

    The internal bremsstrahlung (IB) spectrum accompanying the β-decay from 185W radionuclide has been measured. The obtained spectrum was analyzed into its constituting gamma lines taking into account all the proper corrections. The analyzed spectrum was compared with those theoretically calculated. The experimental results for the IB probability were found to be in good agreement with theoretical calculations especially with the shape corrected modified KUB theory of Nilsson up to 357.7 keV. (author)

  18. Psycho-pedagogical accompaniment as a condition for social adaptation of inmates of children's homes

    Oksana Mishenko

    2013-06-01

    Full Text Available The research is devoted to the problem of pedagogic-psychological accompaniment of the process of social adaptation of inmates of children's homes. Essence of pedagogic-psychological support as a special kind of professional activity to create conditions conducive to the successful adaptation of the inmates of children's homes in the community. Describes the set of psycho-pedagogical conditions, optimizing the process of social adaptation of inmates of children's homes.

  19. Disseminated Intravascular Coagulation (DIC) Accompanied by Acute Rejection in a Post Renal Transplant Recipient

    Dohi, Kiyohiko; Eto, Takaaki; Ono, Eiji; Fukuda, Yasuhiko; Takenaka, Masaharu; Yahata, Hiroshi; Asahara, Toshimasa; Tabe, Yasuji; Marubayashi, Seiji; Ezaki, Haruo

    1984-01-01

    A kidney transplant recipient with disseminated intravascular coagulation (DIC) accompanied by acute rejection was described. The principal symptome of the patient was massive gross hematuria. She showed thrombocytopenia, marked decrease of fibrinogen and elevation of fibrinogen degradation products (FDP) level. The patient was treated by continuous intravenous heparin infusion (total dose was 85, 800 units), and it was very effective. The symtoms due to DIC were improved on the 9th day after...

  20. Multiple Fractures in Patient with Graves' Disease Accompanied by Isolated Hypogonadotropic Hypogonadism

    Yi, Hyon-Seung; Kim, Ji Min; Ju, Sang Hyeon; Lee, Younghak; Kim, Hyun Jin; Kim, Koon Soon

    2016-01-01

    Isolated hypogonadotropic hypogonadism (IHH) is known to decrease bone mineral density due to deficiency of sex steroid hormone. Graves' disease is also an important cause of secondary osteoporosis. However, IHH does not preclude the development of primary hyperthyroidism caused by Graves' disease, leading to more severe osteoporosis rapidly. Here, we describe the first case of 35-year-old Asian female patient with IHH accompanied by Graves' disease and osteoporosis-induced multiple fractures...

  1. Clinical accompaniment: the critical care nursing students’ experiences in a private hospital

    N. Tsele; Marie Muller

    2000-01-01

    The quality of clinical accompaniment of the student enrolled for the post-basic diploma in Medical and Surgical Nursing Science: Critical Care Nursing (General) is an important dimension of the educational/learning programme. The clinical accompanist/mentor is responsible for ensuring the student’s compliance with the clinical outcomes of the programme in accordance with the requirements laid down by the Nursing Education Institution and the South African Nursing Council. The purpose of this...

  2. A case of superior segmental optic hypoplasia accompanied by a glaucomatous optic neuropathy

    Ohguro, Hiroshi

    2008-01-01

    Ikuyo Ohguro, Hiroshi OhguroDepartment of Ophthalmology, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, JapanAbstract: This is the first case report of a bilateral superior segmental optic hypoplasia (SSOH) accompanied by a glaucomatous optic neuropathy (GON). A 47-year-old man incidentally diagnosed as having bilateral SSOH, simultaneously disclosed glaucomatous optic disc appearances, including enlargements of the cup of the optic nerve heads and a thinning of the infero-...

  3. Multiple dental anomalies accompany unilateral disturbances in abducens and facial nerves: A case report

    Elham Talatahari

    2016-01-01

    Full Text Available This article describes the oral rehabilitation of an 8-year-old girl with extensively affected primary and permanent dentition. This report is unique in which distinct dental anomalies including enamel hypoplasia, irregular dentin formation, taurodontism, hpodontia and dens in dente accompany unilateral disturbance of abducens and facial nerves which control the lateral eye movement, and facial expression, respectively. Keywords: enamel hypoplasia; irregular dentin formation; taurodontism; hypodontia; dens in dente; abducens and facial nerves;

  4. Recurrent dislocation of the patella accompanying hypotrochlea of the femur and malalignment of the patella

    Horikawa, Akira; Kodama, Hiroyuki; Miyakoshi, Naohisa; Yamada, Shin; Miyamoto, Seiya

    2011-01-01

    This case report describes a rare case of recurrent dislocation of the patella which was accompanied with trochlear dysplasia and malalignment of the patella in a 15-year-old girl. She complained of hemoarthrosis and recurrent patellar dislocation in the early knee flexion phase. Plain radiography and computed tomography (CT) showed patellar malalignment (quadriceps angle 20°) and severe dysplasia of the trochlea of the femur (sulcus angle 170°). Surgery was performed, consisting of trochleop...

  5. A qualitative investigation into the characteristics and effects of music accompanying exercise

    Priest, DL; Karageorghis, CI

    2008-01-01

    The purpose of the present study was to identify the characteristics of music used to accompany physical exercise and investigate the effects of such music using a qualitative approach. This work underpins the further development of a theoretical structure that is still relatively new. Semi-structured interviews were conducted with a sample of exercise participants (N = 13), seven males and six females, all with at least two years’ experience of exercising to music. An inductive content analy...

  6. Accompanying conditions in patients with chronic spontaneous urticaria and urticarial vasculitis: Results of a retrospective study

    Sevgi Akarsu; Turna İlknur; Özlem Özbağçıvan; Emel Fetil

    2015-01-01

    Background and Design: Chronic spontaneous urticaria (CSU), the most common form of chronic urticaria, is characterized by spontaneous wheals and/or angioedema lasting longer than six weeks. Urticarial vasculitis (UV) is a small vessel vasculitis; but is also included in the various classification systems of chronic urticaria by some authors. The aim of our study was to evaluate the frequency of accompanying conditions, and to compare the demographic, clinical and laboratory features of patie...

  7. The effect of mechanical lymph drainage accompanied with heat on lymphedema

    Valente Flávia Mariana; Guerreiro Godoy Maria de Fátima; Pereira de Godoy José Maria

    2011-01-01

    Background: Thermotherapy has been indicated by some researchers as a treatment for lymphedema. A study comparing temperatures demonstrated that a temperature of 40°C significantly increased the transportation of lymph compared to other temperatures assessed. The aim of this study was to evaluate the possible benefits of mechanical lymph drainage accompanied with heat in the treatment of lymphedema of the lower limbs. Methods: In a cross-over randomized study, the effect of heat on lymph ...

  8. Pedagogical guidelines for educational accompaniment for grieving to adults with intellectual disabilities

    Pablo RODRÍGUEZ HERRERO; de la Herrán Gascón, Agustín; Izuzquiza Gasset, Dolores

    2013-01-01

    It is needed a foundation and some orientations to act in loss and grief situations with adults with intellectual disabilities. In this article, through a relevant literature review, we based the educational accompaniment as a pedagogic methodology of support with three principal elements: a) The conception of grief from its formative potential, b) The prevention of disorders associated to grief complications, c) The pedagogic intervention preferably from the tutorship or from the actuation o...

  9. Modified Latarjet Procedure for Patients with Glenoid Bone Defect Accompanied with Anterior Shoulder Instability.

    Demirhan, Mehmet Selahattin; Atalar, Ata Can; Bilsel, Kerem; Eren, İlker; Çelik, Derya; Çil, Hilal

    2013-01-01

    Abstract: Objective: The aim of this study was to assess the effects of coracoid bone block (modified Latarjet) procedure on clinical and functional results in cases with glenoid bone defect accompanied with anterior shoulder instability. Methods: The study included 35 patients (average age: 35 years; range: 20 to 58 years) with glenoid bone defect and recurrent dislocations treated with the modified Latarjet procedure. There were 12 sports injuries, 5 post-epileptic cases and 18 recurrent an...

  10. Original compositions using contemporary classical and jazz techniques accompanied by technical analysis Vol. I

    Wijers, Dennis

    2011-01-01

    This portfolio of compositions consists of six major works and is accompanied by a technical analysis of each. Nature, the sky, astronomy, clouds and many fields of science have always fascinated me and four of the works presented here are heavily influenced by this fascination. The two remaining works are inspired by my interest in computing, computer programming and the possibilities opened up by the use of computers in composition. The first work, title Jupiter Moons Suite is a five...

  11. Leaching of gold, silver and accompanying metals from circuit boards (PCBs) waste

    Jana Ficeriová; Peter Baláž; Eberhard Gock

    2011-01-01

    Au-Ag noble metal wastes represent a wide range of waste types and forms, with various accompanying metallic elements.The presented leaching strategy for Au-Ag contained in circuit boards (PCBs) aims at gaining gold and silver in the metallic form.Application of the proposed ammonium thiosulphate leaching process for the treatment of the above mentioned Au-Ag containing wastesrepresents a practical, economic and at the same time an ecological solution. The ammonium thiosulphate based leaching...

  12. Explanatory notes to accompany the Groundwater Vulnerability Index GIS for Moray Council

    O Dochartaigh, B.E.

    2003-01-01

    These notes are designed to accompany the ArcView geographical information system (GIS) format groundwater vulnerability index map produced by the British Geological Survey (BGS) for Moray Council. The map is based on digital geological information for both bedrock and superficial (drift) deposits. It covers the whole of the Moray Council area plus a ‘buffer zone’ around the landward boundaries to account for peripheral data and allow for more meaningful interpretation. The ...

  13. INTEGRATED WASTE WATER TREATMENT ACCOMPANIED BY MINIMAL GENERATION OF EXCESSIVE ACTIVATED SLUDGE OR SEDIMENT

    Makisha Nikolay Alekseevich

    2012-12-01

    ments held. A combination of aerobic and anaerobic processes helps provide the proper quality of integrated biological treatment. Chambers of the aeration reactor are also equipped with the polymer feed of various compositions. Sludge treatment that is also strongly needed was performed by means of aerobic stabilization accompanied by ejecting aeration. The experiment findings demonstrate its substantial effect in terms of both components, including sewage and sludge treatment.

  14. Does interdependence alleviate proliferation risks in Asia?

    As the extension Conference of the Non-Proliferation Treaty ended with its unlimited extension without any modification of the treaty obligation of the nuclear-weapon states to nuclear disarmament, the prospect of the regime to incorporate those suspected nuclear powers in Asia, namely India, Pakistan and Israel, has become dark. In this paper the problem of proliferation risk centred in India is analysed. It is well known that India was the first to criticize the Non-Proliferation Treaty regime as highly discriminating between the five nuclear haves and the rest. As was always the case, India's point is logically persuasive and consistent. However, it is also true that the logical or legal point of view does not always solve the problem. Therefore, we look at the regional and international political constellation in which any assertion has to be constructed. At present there is very little possibility that this region will become nuclear-free. This is because the horizontal proliferation, that has seemingly taken place here, is the result of a 'nuclear chain reaction', starting with a minor nuclear-weapon state, China. However, the economic upsurge in the region now throws the nuclear chain-reactions into the background. It has come to a standstill and the future of nuclear proliferation here will have to be decided by global nuclear disarmament, as there is no motivation on the side of the two Asian giants to agree on the reduction in their nuclear arsenals, though under American pressure, another suspected nuclear power, Pakistan, may have to give up its nuclear development plan

  15. Proliferation of luteal steroidogenic cells in cattle.

    Shin Yoshioka

    Full Text Available The rapid growth of the corpus luteum (CL after ovulation is believed to be mainly due to an increase in the size of luteal cells (hypertrophy rather than an increase in their number. However, the relationship between luteal growth and the proliferation of luteal steroidogenic cells (LSCs is not fully understood. One goal of the present study was to determine whether LSCs proliferate during CL growth. A second goal was to determine whether luteinizing hormone (LH, which is known have roles in the proliferation and differentiation of follicular cells, also affects the proliferation of LSCs. Ki-67 (a cell proliferation marker was expressed during the early, developing and mid luteal stages and some Ki-67-positive cells co-expressed HSD3B (a steroidogenic marker. DNA content in LSCs isolated from the developing CL increased much more rapidly (indicating rapid growth than did DNA content in LSCs isolated from the mid CL. The cell cycle-progressive genes CCND2 (cyclin D2 and CCNE1 (cyclin E1 mRNA were expressed more strongly in the small luteal cells than in the large luteal cells. LH decreased the rate of increase of DNA in LSCs isolated from the mid luteal stage but not in LSCs from the developing stage. LH suppressed CCND2 expression in LSCs from the mid luteal stage but not from the developing luteal stage. Furthermore, LH receptor (LHCGR mRNA expression was higher at the mid luteal stage than at the developing luteal stage. The overall results suggest that the growth of the bovine CL is due to not only hypertrophy of LSCs but also an increase in their number, and that the proliferative ability of luteal steroidogenic cells decreases between the developing and mid luteal stages.

  16. Farnesoid X receptor activation promotes cell proliferation via PDK4-controlled metabolic reprogramming

    Xie, Yang; Wang, Hong; Cheng, Xuefang; Wu, Yuzheng; Cao, Lijuan; Wu, Mengqiu; Xie, Wen; Wang, Guangji; Hao, Haiping

    2016-01-01

    Farnesoid X receptor (FXR) plays a pivotal role in the regulation of various metabolic pathways as well as liver regeneration. However, the casual link between cell proliferative effects during liver regeneration and metabolic regulation of FXR was elusive. In this study, we found that FXR activation significantly promotes HepG2 cell proliferation accompanied with metabolic switch towards the excessive accumulation of aerobic glycolytic intermediates including lactic acid, pyruvate and the subsequently increased biosynthesis of glycine. This FXR-induced metabolic switch was found dependent on an up-regulation of pyruvate dehydrogenate kinase 4 (PDK4), a FXR target gene. FXR agonists were found to promote liver regeneration in the murine model of APAP induced liver injury, which was associated with a metabolic switch favoring the accumulation of glycolytic intermediates as precursors for generation of biomass. However, FXR activation has little effect on the glycolytic metabolism in healthy primary hepatocytes in vitro and the liver of healthy mice in vivo. Therefore, we conclude that FXR may promote the proliferation of tumor cells and the hepatocytes in the process of liver regeneration by activating the PDK4-mediated metabolic reprogramming to generate glycolytic intermediates essential for rapid biomass generation, establishing a mechanistic link between cell proliferation and metabolic switch. PMID:26728993

  17. Antidiabetic drug metformin inhibits esophageal adenocarcinoma cell proliferation in vitro and in vivo.

    Fujihara, Shintaro; Kato, Kiyohito; Morishita, Asahiro; Iwama, Hisakazu; Nishioka, Tomoko; Chiyo, Taiga; Nishiyama, Noriko; Miyoshi, Hisaaki; Kobayashi, Mitsuyoshi; Kobara, Hideki; Mori, Hirohito; Okano, Keiichi; Suzuki, Yasuyuki; Masaki, Tsutomu

    2015-05-01

    Esophageal carcinoma is the eighth most common cancer worldwide and the sixth leading cause of cancer-related deaths, with one of the worst prognoses of any form of cancer. Treatment with the anti-diabetic drug metformin has been associated with reduced cancer incidence in patients with type 2 diabetes. This study therefore evaluated the effects of metformin on the proliferation, in vitro and in vivo, of human esophageal adenocarcinoma cells, as well as the microRNAs associated with the antitumor effects of metformin. Metformin inhibited the proliferation of the esophageal adenocarcinoma cell lines OE19, OE33, SK-GT4 and OACM 5.1C, blocking the G0 to G1 transition in the cell cycle. This was accompanied by strong reductions in G1 cyclins, especially cyclin D1, cyclin-dependent kinase (Cdk)4, and Cdk6, and decreases in retinoblastoma protein phosphorylation. In addition, metformin reduced the phosphorylation of epidermal growth factor receptor and insulin-like growth factor and insulin-like growth factor-1 receptor, as well as angiogenesis-related proteins, such as vascular endothelial growth factor, tissue inhibitor of metalloproteinases (TIMP)-1, and TIMP-2. Metformin also markedly altered microRNA expression. Treatment with metformin of athymic nude mice bearing xenograft tumors reduced tumor proliferation. These findings suggest that metformin may have clinical use in the treatment of esophageal adenocarcinoma. PMID:25709052

  18. The Antidiabetic Drug Metformin Inhibits the Proliferation of Bladder Cancer Cells in Vitro and in Vivo

    Tao Zhang

    2013-12-01

    Full Text Available Recent studies suggest that metformin, a widely used antidiabetic agent, may reduce cancer risk and improve prognosis of certain malignancies. However, the mechanisms for the anti-cancer effects of metformin remain uncertain. In this study, we investigated the effects of metformin on human bladder cancer cells and the underlying mechanisms. Metformin significantly inhibited the proliferation and colony formation of 5637 and T24 cells in vitro; specifically, metformin induced an apparent cell cycle arrest in G0/G1 phases, accompanied by a strong decrease of cyclin D1, cyclin-dependent kinase 4 (CDK4, E2F1 and an increase of p21waf-1. Further experiments revealed that metformin activated AMP-activated protein kinase (AMPK and suppressed mammalian target of rapamycin (mTOR, the central regulator of protein synthesis and cell growth. Moreover, daily treatment of metformin led to a substantial inhibition of tumor growth in a xenograft model with concomitant decrease in the expression of proliferating cell nuclear antigen (PCNA, cyclin D1 and p-mTOR. The in vitro and in vivo results demonstrate that metformin efficiently suppresses the proliferation of bladder cancer cells and suggest that metformin may be a potential therapeutic agent for the treatment of bladder cancer.

  19. Measurement of the specific airway resistance by plethysmography in young children accompanied by an adult

    Klug, B; Bisgaard, H

    1997-01-01

    The purpose of this study was to evaluate a procedure for measurement of specific airway resistance (sRaw) by whole body plethysmography in young awake children accompanied by an adult. sRaw was measured by a single-step procedure, omitting the measurement of the thoracic gas volume. The frequency...... performed measurements with and without an accompanying adult, the mean value of sRaw was 1.45 (0.36) and 1.44 (0.38) kPa x s, respectively, with a mean difference of 0.008 (0.152) kPa x s, and mean within-subject coefficients of variations (CV) of 8% and 10%, respectively. In 52 children (mean age 3.3 (0.......8) yrs), for whom measurements made only in the presence of an accompanying adult, the CV was 8.5%. No measurements could be obtained in 22 children (17%) (mean age 2.8 (0.5) yrs). Measurements exhibited a significant frequency dependency, and electronic BTPS compensation substantially overestimated SRaw...

  20. [Physiognomy-accompanying auditory hallucinations in schizophrenia: psychopathological investigation of 10 patients].

    Nagashima, Hideaki; Kobayashi, Toshiyuki

    2010-01-01

    We previously reported two schizophrenic patients with characteristic hallucinations consisting of auditory hallucinations accompanied by visual hallucinations of the speaker's face. The patient sees the face of the hallucinatory speaker in his/her mind and hears the voice talking inwardly. We termed these experiences physiognomy-accompanying auditory hallucinations. In this report, we present 10 patients with schizophrenia showing physiognomy-accompanying auditory hallucinations and evaluate the characteristics of these clinical symptoms. Moreover we consider what the symptoms mean for patients and the metabasis from structural aspects. Lastly, we consider how we can treat these patients living autistic lives with the symptoms. During physiognomy-accompanying auditory hallucinations, the realistic face moves its mouth and talks to the patient expressively. In early onset cases, the faces of various real people appear talking about ordinary things while in late onset cases, the faces can be imaginary but are mainly real people talking about ordinary or delusional things. We suppose that these characteristics of the symptoms unify the schizophrenic world overwhelmed by "a force of non-sense" to "the sense field". "The force of non-sense" is a substantial power but cannot be reduced to the real meaning. And we suppose that not visual reality but the intensity of auditory hallucinations of the face brings about the overwhelming intensity of symptoms and the substantiality of this intensity depends on the states of excessive fullness of "the force of non-sense". With these symptoms patients see the narration of auditory hallucinations through the facial image and the content of auditory hallucinations is compressed into the movement of visual hallucinations of the speaker's face. The form of symptoms is realistic but the speaker's face and voice are beyond ordinary time and space. The symptoms are essentially different from ordinary perception. The visual

  1. Postures for non-proliferation. Arms limitation and security policies to minimize nuclear proliferation

    In order to design effective policies to minimize the proliferation of nuclear weapons, it is necessary to examine how the arms limitation and security policies of the major states in the international security system - particularly the nuclear weapon states - affect the decisions which non-nuclear weapon states must make concerning whether or not to acquire an independent nuclear weapon capability. The arms limitation and security policies which might minimize the future proliferation of nuclear weapons to additional states are identified, first, by analysing the debate concerning this relationship in the strategic literature on nuclear proliferation; and second, by analysing the discussions concerning this relationship which have gone on between the nuclear weapon states and the non-nuclear weapon states during negotiations on the Treaty on the Non-Proliferation of Nuclear Weapons (the NPT) of 1968 and at the Review Conference of the NPT held in 1975. (author)

  2. Which future for the nuclear counter-proliferation?; Quel avenir pour la contre-proliferation nucleaire?

    Duval, M

    2004-10-01

    After a recall of the permanent data about proliferation and of the safeguards implemented by the international community, the author demonstrates that proliferation has moved towards Asia where a real 'black market' has been created. Then he analyzes the consequences of this change on the future of nuclear deterrent. Finally, he expresses his nostalgia in front of this drift and worries about the future uselessness of the means devoted to this 'pacifying' strategy. (J.S.)

  3. Proliferating cell nuclear antigen: a marker for hepatocellular proliferation in rodents.

    Eldrige, S R; Butterworth, B E; Goldsworthy, T L

    1993-01-01

    Two different markers for quantitating cell proliferation were evaluated in livers of control and chemically treated mice and rats. Proliferating cell nuclear antigen (PCNA), an endogenous cell replication marker, and bromodeoxyuridine (BrdU), an exogenously administered DNA precursor label, were detected in formalin-fixed, paraffin-embedded tissues using immunohistochemical techniques. The percentage of cells in S phase (labeling indexes, LI) evaluated as PCNA- or BrdU-positive hepatocellula...

  4. Phosphorylation at tyrosine 114 of Proliferating Cell Nuclear Antigen (PCNA) is required for adipogenesis in response to high fat diet

    Lo, Yuan-Hung; Ho, Po-Chun; Chen, Min-Shan; Hugo, Eric; Ben-Jonathan, Nira [Department of Cancer Biology, University of Cincinnati College of Medicine, 3125 Eden Avenue, Cincinnati, OH 45267-0521 (United States); Department of Environmental Health, University of Cincinnati College of Medicine, 3223 Eden Avenue, Kettering Laboratory, Cincinnati, OH 45267-0056 (United States); Wang, Shao-Chun, E-mail: shao-chun.wang@uc.edu [Department of Cancer Biology, University of Cincinnati College of Medicine, 3125 Eden Avenue, Cincinnati, OH 45267-0521 (United States); Department of Environmental Health, University of Cincinnati College of Medicine, 3223 Eden Avenue, Kettering Laboratory, Cincinnati, OH 45267-0056 (United States)

    2013-01-04

    Highlights: Black-Right-Pointing-Pointer Proliferating Cell Nuclear Antigen (PCNA) is phosphorylated at Y114. Black-Right-Pointing-Pointer Phospho-Y114 of PCNA is not required for cell proliferation for normal growth. Black-Right-Pointing-Pointer MCE during adipogenesis is abolished in the lack of the phosphorylation. Black-Right-Pointing-Pointer Homozygous Y114F mice are resistant to high fat diet induced obesity. Black-Right-Pointing-Pointer Our results shed light on the interface between proliferation and differentiation. -- Abstract: Clonal proliferation is an obligatory component of adipogenesis. Although several cell cycle regulators are known to participate in the transition between pre-adipocyte proliferation and terminal adipocyte differentiation, how the core DNA synthesis machinery is coordinately regulated in adipogenesis remains elusive. PCNA (Proliferating Cell Nuclear Antigen) is an indispensable component for DNA synthesis during proliferation. Here we show that PCNA is subject to phosphorylation at the highly conserved tyrosine residue 114 (Y114). Replacing the Y114 residue with phenylalanine (Y114F), which is structurally similar to tyrosine but cannot be phosphorylated, does not affect normal animal development. However, when challenged with high fat diet, mice carrying homozygous Y114F alleles (PCNA{sup F/F}) are resistant to adipose tissue enlargement in comparison to wild-type (WT) mice. Mouse embryonic fibroblasts (MEFs) harboring WT or Y114F mutant PCNA proliferate at similar rates. However, when subjected to adipogenesis induction in culture, PCNA{sup F/F} MEFs are not able to re-enter the cell cycle and fail to form mature adipocytes, while WT MEFs undergo mitotic clonal expansion in response to the adipogenic stimulation, accompanied by enhanced Y114 phosphorylation of PCNA, and differentiate to mature adipocytes. Consistent with the function of Y114 phosphorylation in clonal proliferation in adipogenesis, fat tissues isolated from WT

  5. Phosphorylation at tyrosine 114 of Proliferating Cell Nuclear Antigen (PCNA) is required for adipogenesis in response to high fat diet

    Highlights: ► Proliferating Cell Nuclear Antigen (PCNA) is phosphorylated at Y114. ► Phospho-Y114 of PCNA is not required for cell proliferation for normal growth. ► MCE during adipogenesis is abolished in the lack of the phosphorylation. ► Homozygous Y114F mice are resistant to high fat diet induced obesity. ► Our results shed light on the interface between proliferation and differentiation. -- Abstract: Clonal proliferation is an obligatory component of adipogenesis. Although several cell cycle regulators are known to participate in the transition between pre-adipocyte proliferation and terminal adipocyte differentiation, how the core DNA synthesis machinery is coordinately regulated in adipogenesis remains elusive. PCNA (Proliferating Cell Nuclear Antigen) is an indispensable component for DNA synthesis during proliferation. Here we show that PCNA is subject to phosphorylation at the highly conserved tyrosine residue 114 (Y114). Replacing the Y114 residue with phenylalanine (Y114F), which is structurally similar to tyrosine but cannot be phosphorylated, does not affect normal animal development. However, when challenged with high fat diet, mice carrying homozygous Y114F alleles (PCNAF/F) are resistant to adipose tissue enlargement in comparison to wild-type (WT) mice. Mouse embryonic fibroblasts (MEFs) harboring WT or Y114F mutant PCNA proliferate at similar rates. However, when subjected to adipogenesis induction in culture, PCNAF/F MEFs are not able to re-enter the cell cycle and fail to form mature adipocytes, while WT MEFs undergo mitotic clonal expansion in response to the adipogenic stimulation, accompanied by enhanced Y114 phosphorylation of PCNA, and differentiate to mature adipocytes. Consistent with the function of Y114 phosphorylation in clonal proliferation in adipogenesis, fat tissues isolated from WT mice contain significantly more adipocytes than those isolated from PCNAF/F mice. This study identifies a critical role for PCNA in adipose

  6. Newly identified interfibrillar collagen crosslinking suppresses cell proliferation and remodelling.

    Marelli, Benedetto; Le Nihouannen, Damien; Hacking, S Adam; Tran, Simon; Li, Jingjing; Murshed, Monzur; Doillon, Charles J; Ghezzi, Chiara E; Zhang, Yu Ling; Nazhat, Showan N; Barralet, Jake E

    2015-06-01

    Copper is becoming recognised as a key cation in a variety of biological processes. Copper chelation has been studied as a potential anti-angiogenic strategy for arresting tumour growth. Conversely the delivery of copper ions and complexes in vivo can elicit a pro-angiogenic effect. Previously we unexpectedly found that copper-stimulated intraperitoneal angiogenesis was accompanied by collagen deposition. Here, in hard tissue, not only was healing accelerated by copper, but again enhanced deposition of collagen was detected at 2 weeks. Experiments with reconstituted collagen showed that addition of copper ions post-fibrillogenesis rendered plastically-compressed gels resistant to collagenases, enhanced their mechanical properties and increased the denaturation temperature of the protein. Unexpectedly, this apparently interfibrillar crosslinking was not affected by addition of glucose or ascorbic acid, which are required for crosslinking by advanced glycation end products (AGEs). Fibroblasts cultured on copper-crosslinked gels did not proliferate, whereas those cultured with an equivalent quantity of copper on either tissue culture plastic or collagen showed no effect compared with controls. Although non-proliferative, fibroblasts grown on copper-cross-linked collagen could migrate, remained metabolically active for at least 14 days and displayed a 6-fold increase in Mmps 1 and 3 mRNA expression compared with copper-free controls. The ability of copper ions to crosslink collagen fibrils during densification and independently of AGEs or Fenton type reactions is previously unreported. The effect on MMP susceptibility of collagen and the dramatic change in cell behaviour on this crosslinked ECM may contribute to shedding some light on unexplained phenomena as the apparent benefit of copper complexation in fibrotic disorders or the enhanced collagen deposition in response to localised copper delivery. PMID:25907046

  7. Wp specific methylation of highly proliferated LCLs

    The epigenetic regulation of viral genes may be important for the life cycle of EBV. We determined the methylation status of three viral promoters (Wp, Cp, Qp) from EBV B-lymphoblastoid cell lines (LCLs) by pyrosequencing. Our pyrosequencing data showed that the CpG region of Wp was methylated, but the others were not. Interestingly, Wp methylation was increased with proliferation of LCLs. Wp methylation was as high as 74.9% in late-passage LCLs, but 25.6% in early-passage LCLs. From two Burkitt's lymphoma cell lines, Wp specific hypermethylation was also found (>80%). Interestingly, the expression of EBNA2 gene which located directly next to Wp was associated with its methylation. Our data suggested that Wp specific methylation may be important for the indicator of the proliferation status of LCLs, and the epigenetic viral gene regulation of EBNA2 gene by Wp should be further defined possibly with other biological processes

  8. Flaws in the Non-Proliferation Treaty

    The current review conference must come to grips with serious weaknesses in the Non-Proliferation Treaty (NPT), especially regarding the spread of weapons-grade material and the continuing superpower arms race. One-third of the world's nations, including two of the original nuclear-weapons states (France and China), have not signed the NPT, and some countries are trying to buy nuclear weaponry without developing a domestic capability. The greatest danger is latent proliferation through the acquisition of materials and capabilities for peaceful power and research programs. The author recommends modifying the NPT to restrict nuclear trade, improve International Atomic Energy Agency inspections and audits, expand safeguards coverage to nonparties of the Treaty, and set minimum standards for guarding international shipments

  9. Proliferation Persuasion. Coercive Bargaining with Nuclear Technology

    Volpe, Tristan A. [George Washington Univ., Washington, DC (United States)

    2015-08-31

    Why do states wait for prolonged periods of time with the technical capacity to produce nuclear weapons? Only a handful of countries have ever acquired the sensitive nuclear fuel cycle technology needed to produce fissile material for nuclear weapons. Yet the enduring trend over the last five decades is for these states to delay or forgo exercising the nuclear weapons option provided by uranium enrichment or plutonium reprocessing capabilities. I show that states pause at this threshold stage because they use nuclear technology to bargain for concessions from both allies and adversaries. But when does nuclear latency offer bargaining benefits? My central argument is that challengers must surmount a dilemma to make coercive diplomacy work: the more they threaten to proliferate, the harder it becomes to reassure others that compliance will be rewarded with nuclear restraint. I identify a range of mechanisms able to solve this credibility problem, from arms control over breakout capacity to third party mediation and confidence building measures. Since each step towards the bomb raises the costs of implementing these policies, a state hits a sweet spot when it first acquires enrichment and/or reprocessing (ENR) technology. Subsequent increases in proliferation capability generate diminishing returns at the bargaining table for two reasons: the state must go to greater lengths to make a credible nonproliferation promise, and nuclear programs exhibit considerable path dependency as they mature over time. Contrary to the conventional wisdom about power in world politics, less nuclear latency thereby yields more coercive threat advantages. I marshal new primary source evidence from archives and interviews to identify episodes in the historical record when states made clear decisions to use ENR technology as a bargaining chip, and employ this theory of proliferation persuasion to explain how Japan, North Korea, and Iran succeeded and failed to barter concessions from the

  10. The Antarctic - Zangger of proliferation control

    Meadley, T. [Uranium Inst., London (United Kingdom)

    1995-08-01

    The Treaty for the Non-Proliferation of Nuclear Weapons is the main multi-lateral agreement that aims to limit the spread of nuclear weapons. But it is not the only one. In this, the first in an occasional series of reference guides, Core Issues focuses on the various antiproliferation agreements, what they mean and who is party to them. The author is Tim Meadley, Head of External Communications at the UI, with additional research by Charu Jasani. (UK).

  11. Director`s series on proliferation

    Bailey, K.C.; Price, M.E. [eds.

    1994-10-17

    This series is an occasional publication of essays on the topics of nuclear, chemical, biological, and missile proliferation. Essays contained in this document include: Key issues on NPT renewal and extension, Africa and nuclear nonproliferation, Kenya`s views on the NPT, Prospects for establishing a zone free of weapons of mass destruction in the middle east, effects of a special nuclear weapon materials cut-off convention, and The UK view of NPT renewal.

  12. IAEA safeguards and non-proliferation

    Harry, R.J.S.

    1995-02-01

    An overview is given of efforts to contain the nuclear weapons proliferation during half a century of man-controlled nuclear fission. An initial policy of denial did not work, a following period of cooperation needed a gradual strengthening of international assurances on the peaceful character of the flourishing use of nuclear techniques for power generation and of other applications. The focus of the nuclear weapon proliferation concern changed from the highly developed states to developing states. The Non-Proliferation Treaty laid the basis for a unique system of voluntarily accepted international inspections to verify the peaceful use of nuclear energy. The IAEA got the task to implement this `Full Scope Safeguards` on all nuclear material and all nuclear activities in the non-nuclear weapon states. Thanks to the structure of the IAEA, in which both proponent and states with a critical attitude take part in the decision making process on the IAEA execution of its tasks, a balanced, and widely acceptable system emerged. International developments necessitated additional improvements of the non-proliferation system. The increase of strength of sub-national groups triggered international cooperation on physical protection, about a quarter of a century ago. More recently, it appeared that NPT states with assumed nuclear weapon ambitions operated in the margins between the interpretation of IAEA safeguards and the spirit and purpose of NPT. Improvements of the IAEA safeguards and a stronger cooperation between states, including the constraints which exporting states have imposed on nuclear supplies, strengthen the safeguards system. The important reductions in the two largest nuclear weapon arsenals lead, together with the delay in the fast breeder implementation, to large stockpiles of nuclear weapon usable materials. Also in this areas new internationally credible assurances have to be obtained, that these materials will never return to nuclear weapon applications.

  13. Overcoming challenges to the proliferation security initiative

    Warden, Herbert N.

    2004-01-01

    A U.S.-led naval operation in October 2003 interdicted a shipment of uranium-enrichment components on-board a German cargo ship traveling from Dubai to Libya. In December 2003, Libya announced it would halt its weapons of mass destruction (WMD) programs and eliminate its existing stockpiles under international verification and supervision. The George W. Bush Administration proclaimed the interdiction a triumph for the newly created Proliferation Security Initiative (PSI), an activity which wa...

  14. IAEA safeguards and non-proliferation

    An overview is given of the efforts to contain the nuclear weapons proliferation during half a century of man-controlled nuclear fission. An initial policy of denial did not work, a following period of cooperation needed a gradual strengthening of international assurances on the exclusively peaceful character of the flourishing use of nuclear techniques for power generation and of other applications. The focus of the nuclear weapon proliferation concern changed from the highly developed states to developing states. The Non-Proliferation Treaty laid the basis for a unique system of voluntarily accepted international inspections to verify the peaceful use of nuclear energy. The IAEA got the task to implement this 'Full Scope Safeguards' on all nuclear material and all nuclear activities in the non-nuclear weapon states. Thanks to the structure of the IAEA, in which both proponent and states with a critical attitude take part in the decision making process on the IAEA execution of its tasks, a balanced, and widely acceptable system emerged. International developments necessitated additional improvements of the non-proliferation system. The increase of strength of sub-national groups triggered international cooperation on physical protection, about a quarter of a century ago. More recently, it appeared that NPT states with assumed nuclear weapon ambitions operated in the margins between the interpretation of IAEA safeguards and the spirit and purpose of NPT. Improvements of the IAEA safeguards and a stronger cooperation between states, including the constraints which exporting states have imposed on nuclear supplies, strengthen the safeguards system. The important reductions in the two largest nuclear weapon arsenals lead, together with the delay in the fast breeder implementation, to large stockpiles of nuclear weapon usable materials. Also in this areas new internationally credible assurances have to be obtained, that these materials will never return to nuclear

  15. Security Guarantees and Nuclear Non-Proliferation

    The purpose of this paper is to discuss the value of 'security guarantees', that is, positive security assurances that include a formal or informal defense commitment, in preventing nuclear proliferation. It demonstrates that such guarantees have proven to be a very effective instrument in preventing States from going nuclear. It would thus seem logical to reinforce or extend them. However, this path is fraught with obstacles and dilemmas

  16. Balancing non-proliferation and energy security

    The subject is covered in sections, entitled: energy security problems (forecasting energy needs and supplies); nuclear energy and non-proliferation (plutonium production and fuel reprocessing); U.S. nuclear energy strategy (deferring commercialization of plutonium, International Nuclear Fuel Cycle Evaluation (INFCE)); the uranium issue (world resources and supplies of uranium); new technology; uranium and the role of plutonium; prospects for stability after INFCE. (U.K.)

  17. Solutions for nuclear non-proliferation

    The authors present the solution of problems associated with improvement of accounting systems, physical protection of nuclear materials on Siberian Group of Chemical Enterprises that is a good example of specific steps undertaking to ensure global nuclear non-proliferation. Upgrades to security systems, implemented with the assistance of USA specialists, was aimed at ensuring practical protection of actual nuclear materials at a large industrial complex

  18. Mitochondrial Regulation of Cell Cycle and Proliferation

    Antico Arciuch, Valeria Gabriela; Elguero, María Eugenia; Poderoso, Juan José; Carreras, María Cecilia

    2012-01-01

    Eukaryotic mitochondria resulted from symbiotic incorporation of α-proteobacteria into ancient archaea species. During evolution, mitochondria lost most of the prokaryotic bacterial genes and only conserved a small fraction including those encoding 13 proteins of the respiratory chain. In this process, many functions were transferred to the host cells, but mitochondria gained a central role in the regulation of cell proliferation and apoptosis, and in the modulation of metabolism; accordingly...

  19. Cell adhesion and proliferation on modified polyethylene

    Kasálková, N.; Kolářová, K.; Bačáková, Lucie; Pařízek, Martin; Macková, Anna; Švorčík, V.

    ZURICH: TRANS TECH PUBLICATIONS LTD, 2008 - (Sandera, P.), s. 269-272. (MATERIALS SCIENCE FORUM. 567-568). ISSN 0255-5476. [5th International Conference on Materials Structure & Micromechanics of Fracture. Brno (CZ), 27.06.2007-29.06.2007] Institutional research plan: CEZ:AV0Z10480505; CEZ:AV0Z50110509 Keywords : polyethylene * plasma modification * cell adhesion and proliferation Subject RIV: BO - Biophysics

  20. Proliferation resistance assessment of nuclear systems

    The paper focuses on examining the degree to which nuclear systems could be used to acquire nuclear weapons material. It establishes a framework for proliferation resistance assessment and illustrates its applicability through an analysis of reference systems for once-through cycles, breeder cycles and thermal recycle. On a more tentative basis, the approach is applied to various alternative technical and institutional measures. This paper was also submitted to Working Groups 5 and 8

  1. Proliferation networks: between Sopranos and Supermarket

    One success in the struggle against proliferation during the last decade has been that Western countries have reinforced mechanisms for control over exports of goods and technologies intended for the perfection of weapons of mass destruction. These new constraints have probably resulted in the appearance of a genuine proliferation economy, partly underground, organized around contacts between acquisition and sales networks, searching to exploit weaknesses in existing control systems to obtain wanted goods and technologies. This phenomenon is particularly worrying, because the level of technical skills attained by some suppliers is sufficiently high to guarantee that their customers will have a functional product satisfying their demand. Apart from systematic exploitation of vulnerabilities in export control systems, the capacity of these networks of suppliers to conduct their operations is reinforced by access to technologies and globalization of the market and financial tools. This article is the first of a series of two and explores the operation of these proliferation networks. The second article will be dedicated to an analysis of existing tools or other tools that can be implemented to combat them. (authors)

  2. Nuclear non-proliferation: failures and prospects

    The objective of this paper is to examine the evolution of combined political and technical attempts to achieve worldwide acceptance of a commitment to non-proliferation, to note failures to date, and to identify essential factors to be satisfied if greater and necessary success is to be achieved in the immediate future. For this it is necessary to separate the realism and unrealism so often involved in discussing the concept of non-proliferation, as defined above, particularly if treated as a moral principle rather than as part of a general security issue reflecting shifts in regional and global stability. The political nature of the non-proliferation problem is underlined by the fact that whereas five nuclear weapon states are currently accepted, any threatened increase in that number is discouraged by every possible peaceful means. This fact combines political acceptance of an existing international situation with a belief that any addition to the present number must lead to international instability. Success in preventing additions may be more readily achieved through political understanding and perhaps some compromises, in particular cases, rather than through seeking a universal solution to a generalized problem

  3. Elements of safety and non proliferation

    This book on nuclear safety and non proliferation is based on knowledge and expertise of the IRSN. The first chapter addresses the safety of nuclear materials, of their installations and of their transportations. It proposes some contextual elements, presents the general guidelines of the French nuclear safety arrangement, the approach to take risks into account, the involved governmental and public bodies, the legal framework, and the protection and control arrangement (in terms of planning of safety-related activities, in terms of operator obligations, in terms of exercises and management crisis). The second part addresses the safety of radioactive sources: context (peculiarity, losses and thefts), international framework (source categories, Euratom directive), and the French organisation. The third chapter addresses nuclear non proliferation: historical background (creation and role of the IAEA and of the EAEC, definitions), principle of statements, inspection process, and French organisation (legal framework, governmental bodies, the IRSN). The last chapter addresses the issue of chemical non proliferation: historical background, international context (Convention on chemical weapons, organisation for their ban), and the French organisation

  4. Future technology challenges in non-proliferation

    Radiation detection technologies are an important tool in the prevention of proliferation. A variety of new developments have enabled enhanced performance in terms of energy resolution, spatial resolution, predictive modeling and simulation, active interrogation, and ease of operation and deployment in the field. For example, various gamma ray imaging approaches are being explored to combine spatial resolution with background suppression in order to enhance sensitivity at reasonable standoff distances and acquisition times. New materials and approaches are being developed in order to provide adequate energy resolution in field use without the necessity for liquid nitrogen. Finally, different detectors combined into distributed networks offer promise for detection and tracking of radioactive materials. As the world moves into the 21st century, the possibility of greater reliance on nuclear energy will impose additional technical requirements to prevent proliferation. In addition to proliferation resistant reactors, a careful examination of the various possible fuel cycles from cradle to grave will provide additional technical and nonproliferation challenges in the areas of conversion, enrichment, transportation, recycling and waste disposal. Radiation detection technology and information management have a prominent role in any future global regime for nonproliferation beyond the current Advanced Protocol. This work was performed under the auspices of the U.S. Department of Energy by University of California, Lawrence Livermore National Laboratory under contract No. W-7405-Eng-48. (author)

  5. Nuclear cooperation and non-proliferation

    During the years since World War II, few if any areas of vital foreign and national security policy have been the object of such consistency of approach and continuity of effort as has been that of deterring the proliferation of nuclear weapons. The proliferation which has occurred is a matter of grave import. Yet, measured against the diffusion of other new technologies--both civil and military--the acquisition of nuclear explosive capabilities during the 30 years that have passed since the development of the atomic bomb has been encouragingly limited. This is not accidental. It has been, in large measure, the result of U.S. policies and programs and of active U.S. leadership directed toward the containment of national capabilities for the acquisition of nuclear weapons. However, the results do not allow complacency. Concern still exists regarding the growing stockpiles of plutonium and the spread of sensitive technologies such as reprocessing. The results do, nevertheless, give cause for encouragement that proliferation is not inevitable, and that, with the best of effort, it can be contained

  6. Lensless imaging system to quantify cell proliferation

    Vinjimore Kesavan, S.; Allier, C. P.; Navarro, F.; Mittler, F.; Chalmond, B.; Dinten, J.-M.

    2013-02-01

    Owing to its simplicity, lensless imaging system is adept at continuous monitoring of adherent cells inside the incubator. The setup consists of a CMOS sensor with pixel pitch of 2.2 μm and field of view of 24 mm2, LED with a dominating wavelength of 525 nm, along with a pinhole of 150 μm as the source of illumination. The in-line hologram obtained from cells depends on the degree of cell-substrate adhesion. Drastic difference is observed between the holographic patterns of floating and adherent cells. In addition, the well-established fact of reduction of cell-substrate contact during cell division is observed with our system based on corresponding spontaneous transition in the holographic pattern. Here, we demonstrate that by recognizing this specific holographic pattern, number of cells undergoing mitosis in a cell culture with a population of approximately 5000 cells, can be estimated in real-time. The method is assessed on comparison with Edu-based proliferation assay. The approach is straightforward and it eliminates the use of markers to estimate the proliferation rate of a given cell culture. Unlike most proliferation assays, the cells are not harvested enabling continuous monitoring of cell culture.

  7. Valproic acid stimulates proliferation of glial precursors during cortical gliogenesis in developing rat.

    Lee, Hee Jae; Dreyfus, Cheryl; DiCicco-Bloom, Emanuel

    2016-07-01

    Valproic acid (VPA) is a neurotherapeutic drug prescribed for seizures, bipolar disorder, and migraine, including women of reproductive age. VPA is a well-known teratogen that produces congenital malformations in many organs including the nervous system, as well as later neurodevelopmental disorders, including mental retardation and autism. In developing brain, few studies have examined VPA effects on glial cells, particularly astrocytes. To investigate effects on primary glial precursors, we developed new cell culture and in vivo models using frontal cerebral cortex of postnatal day (P2) rat. In vitro, VPA exposure elicited dose-dependent, biphasic effects on DNA synthesis and proliferation. In vivo VPA (300 mg/kg) exposure from P2 to P4 increased both DNA synthesis and cell proliferation, affecting primarily astrocyte precursors, as >75% of mitotic cells expressed brain lipid-binding protein. Significantly, the consequence of early VPA exposure was increased astrocytes, as both S100-β+ cells and glial fibrillary acidic protein were increased in adolescent brain. Molecularly, VPA served as an HDAC inhibitor in vitro and in vivo as enhanced proliferation was accompanied by increased histone acetylation, whereas it elicited changes in culture in cell-cycle regulators, including cyclin D1 and E, and cyclin-dependent kinase (CDK) inhibitors, p21 and p27. Collectively, these data suggest clinically relevant VPA exposures stimulate glial precursor proliferation, though at higher doses can elicit inhibition through differential regulation of CDK inhibitors. Because changes in glial cell functions are proposed as mechanisms contributing to neuropsychiatric disorders, these observations suggest that VPA teratogenic actions may be mediated through changes in astrocyte generation during development. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 780-798, 2016. PMID:26505176

  8. Absence of K-Ras Reduces Proliferation and Migration But Increases Extracellular Matrix Synthesis in Fibroblasts.

    Muñoz-Félix, José M; Fuentes-Calvo, Isabel; Cuesta, Cristina; Eleno, Nélida; Crespo, Piero; López-Novoa, José M; Martínez-Salgado, Carlos

    2016-10-01

    The involvement of Ras-GTPases in the development of renal fibrosis has been addressed in the last decade. We have previously shown that H- and N-Ras isoforms participate in the regulation of fibrosis. Herein, we assessed the role of K-Ras in cellular processes involved in the development of fibrosis: proliferation, migration, and extracellular matrix (ECM) proteins synthesis. K-Ras knockout (KO) mouse embryonic fibroblasts (K-ras(-/-) ) stimulated with transforming growth factor-β1 (TGF-β1) exhibited reduced proliferation and impaired mobility than wild-type fibroblasts. Moreover, an increase on ECM production was observed in K-Ras KO fibroblasts in basal conditions. The absence of K-Ras was accompanied by reduced Ras activation and ERK phosphorylation, and increased AKT phosphorylation, but no differences were observed in TGF-β1-induced Smad signaling. The MEK inhibitor U0126 decreased cell proliferation independently of the presence of K-ras but reduced migration and ECM proteins expression only in wild-type fibroblasts, while the PI3K-AKT inhibitor LY294002 decreased cell proliferation, migration, and ECM synthesis in both types of fibroblasts. Thus, our data unveil that K-Ras and its downstream effector pathways distinctively regulate key biological processes in the development of fibrosis. Moreover, we show that K-Ras may be a crucial mediator in TGF-β1-mediated effects in this cell type. J. Cell. Physiol. 231: 2224-2235, 2016. © 2016 Wiley Periodicals, Inc. PMID:26873620

  9. Low power ultrasound inhibits cell proliferation and invasion of human cancer cells in vitro

    Etienne Mfoumou

    2012-01-01

    Full Text Available Background: Applications of ultrasound in medicine for therapeutic purposes have been accepted, and they have several beneficial uses for many years. However, the outcome of low power ultrasound waves on cell proliferation, especially cell cycle progression and invasion as well as their associated genes on human breast and cervical cancer cells has not been investigated yet. Therefore, we examined the effect of low power ultrasound on BT20, BT20-E6/E7 and HeLa cell lines. Materials and Methods: BT20, BT20-E6/E7 and HeLa cell lines were used in this study. On the other hand, cell proliferation, cell cycle, and invasion assays were applied to study the effect of low ultrasound irradiation on these cell lines. Meanwhile, western blot was performed to study the expression patterns of some selected genes associated with this effect. Results: We found that low power ultrasound inhibits cell proliferation and provokes G0-G1 cell cycle arrest and reduction of S as well as an increase in the G2-M phase of HeLa cells in comparison with the untreated cells. This is accompanied by a down-regulation of Cdk-6 (cyclin dependent kinase which is a major control switch for the cell cycle. Moreover, low power ultrasound inhibits cell invasion and consequently down-regulates the expression of Id-1, caveolin, and EGF-R which are widely considered as main regulators of cell invasion and metastasis of human cancer. Conclusion: These results suggest that application of low power ultrasound on human breast and cervical cancer could be an effective method to reduce cell proliferation and invasion of these cancers.

  10. ZEB2 mediates multiple pathways regulating cell proliferation, migration, invasion, and apoptosis in glioma.

    Songtao Qi

    Full Text Available BACKGROUND: The aim of the present study was to analyze the expression of Zinc finger E-box Binding homeobox 2 (ZEB2 in glioma and to explore the molecular mechanisms of ZEB2 that regulate cell proliferation, migration, invasion, and apoptosis. METHODOLOGY/PRINCIPAL FINDINGS: Expression of ZEB2 in 90 clinicopathologically characterized glioma patients was analyzed by immunohistochemistry. Furthermore, siRNA targeting ZEB2 was transfected into U251 and U87 glioma cell lines in vitro and proliferation, migration, invasion, and apoptosis were examined separately by MTT assay, Transwell chamber assay, flow cytometry, and western blot. RESULTS: The expression level of ZEB2 protein was significantly increased in glioma tissues compared to normal brain tissues (P<0.001. In addition, high levels of ZEB2 protein were positively correlated with pathology grade classification (P = 0.024 of glioma patients. Knockdown of ZEB2 by siRNA suppressed cell proliferation, migration and invasion, as well as induced cell apoptosis in glioma cells. Furthermore, ZEB2 downregulation was accompanied by decreased expression of CDK4/6, Cyclin D1, Cyclin E, E2F1, and c-myc, while p15 and p21 were upregulated. Lowered expression of ZEB2 enhanced E-cadherin levels but also inhibited β-Catenin, Vimentin, N-cadherin, and Snail expression. Several apoptosis-related regulators such as Caspase-3, Caspase-6, Caspase-9, and Cleaved-PARP were activated while PARP was inhibited after ZEB2 siRNA treatment. CONCLUSION: Overexpression of ZEB2 is an unfavorable factor that may facilitate glioma progression. Knockdown ZEB2 expression by siRNA suppressed cell proliferation, migration, invasion and promoted cell apoptosis in glioma cells.

  11. Mechanisms of peroxisome proliferator-induced DNA hypomethylation in rat liver

    Genomic hypomethylation is a consistent finding in both human and animal tumors and mounting experimental evidence suggests a key role for epigenetic events in tumorigenesis. Furthermore, it has been suggested that early changes in DNA methylation and histone modifications may serve as sensitive predictive markers in animal testing for carcinogenic potency of environmental agents. Alterations in metabolism of methyl donors, disturbances in activity and/or expression of DNA methyltransferases, and presence of DNA single-strand breaks could contribute to the loss of cytosine methylation during carcinogenesis; however, the precise mechanisms of genomic hypomethylation induced by chemical carcinogens remain largely unknown. This study examined the mechanism of DNA hypomethylation during hepatocarcinogenesis induced by peroxisome proliferators WY-14,643 (4-chloro-6-(2,3-xylidino)-pyrimidynylthioacetic acid) and DEHP (di-(2-ethylhexyl)phthalate), agents acting through non-genotoxic mode of action. In the liver of male Fisher 344 rats exposed to WY-14,643 (0.1% (w/w), 5 months), the level of genomic hypomethylation increased by ∼2-fold, as compared to age-matched controls, while in the DEHP group (1.2% (w/w), 5 months) DNA methylation did not change. Global DNA hypomethylation in livers from WY-14,643 group was accompanied by the accumulation of DNA single-strand breaks, increased cell proliferation, and diminished expression of DNA methyltransferase 1, while the metabolism of methyl donors was not affected. In contrast, none of these parameters changed significantly in rats fed DEHP. Since WY-14,643 is much more potent carcinogen than DEHP, we conclude that the extent of loss of DNA methylation may be related to the carcinogenic potential of the chemical agent, and that accumulation of DNA single-strand breaks coupled to the increase in cell proliferation and altered DNA methyltransferase expression may explain genomic hypomethylation during peroxisome proliferator

  12. Impact of Male Partner Antenatal Accompaniment on Perinatal Health Outcomes in Developing Countries: A Systematic Literature Review.

    Aguiar, Carolina; Jennings, Larissa

    2015-09-01

    Encouraging male partners to accompany women to antenatal care (ANC) is an important first step in engaging men on maternal and newborn health. However, little is known regarding the impact of male partner antenatal accompaniment beyond HIV-related perinatal outcomes. A systematic review was conducted to synthesize the evidence on the influence of male accompaniment on non-HIV outcomes during pregnancy and into the postpartum period. Eligible studies were published in English from 2003 to 2013 and evaluated the effect of male antenatal accompaniment on perinatal health in a developing country. Four electronic databases and selected reference lists were searched. Out of 84 potential citations retrieved, seven publications were retained for the assessment of male antenatal accompaniment's influence using iterative thematic analysis. During pregnancy, male antenatal accompaniment positively impacted women's knowledge of danger signs, but did not affect birth preparedness, ANC utilization, or miscarriages. During labor and delivery, men's ANC presence was associated with increases in institutional delivery and skilled birth attendance, but with no effect for birth-related outcomes. During the early postnatal period, male antenatal accompaniment was associated with higher uptake of postnatal services, but with mixed effects on breastfeeding and newborn survival. Couples' increased communication on pregnancy care and men's subsequent motivation to ensure safe delivery may explain these observed benefits. Inadequate communication, late accompaniment, or partner type may explain the lack of influence on some outcomes. More efforts are needed to expand the implementation and evaluation of male involvement strategies to improve perinatal health. PMID:25656727

  13. 30 CFR 250.244 - What geological and geophysical (G&G) information must accompany the DPP or DOCD?

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What geological and geophysical (G&G... Operations Coordination Documents (docd) § 250.244 What geological and geophysical (G&G) information must accompany the DPP or DOCD? The following G&G information must accompany your DPP or DOCD: (a)...

  14. 30 CFR 250.214 - What geological and geophysical (G&G) information must accompany the EP?

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What geological and geophysical (G&G... and Information Contents of Exploration Plans (ep) § 250.214 What geological and geophysical (G&G) information must accompany the EP? The following G&G information must accompany your EP: (a)...

  15. The Contribution of Conceptual Change Texts Accompanied by Concept Mapping to Students' Understanding of the Human Circulatory System.

    Sungur, Semra; Tekkaya, Ceren; Geban, Omer

    2001-01-01

    Investigates the contribution of conceptual change texts accompanied by concept mapping instruction to 10th-grade students' understanding of the human circulatory system. Indicates that the conceptual change texts accompanied by concept mapping instruction produced a positive effect on students' understanding of concepts. Concludes that students…

  16. Personality Pathology of Adults with Autism Spectrum Disorder without Accompanying Intellectual Impairment in Comparison to Adults with Personality Disorders

    Strunz, Sandra; Westphal, Linda; Ritter, Kathrin; Heuser, Isabella; Dziobek, Isabel; Roepke, Stefan

    2015-01-01

    Differentiating autism spectrum disorders (ASDs) without accompanying intellectual impairment from personality disorders is often challenging. Identifying personality traits and personality pathology specific to ASD might facilitate diagnostic procedure. We recruited a sample of 59 adults with ASD without accompanying intellectual impairment, 62…

  17. [About the last transit. Accompaniment of the soul and the personality].

    Pilar Fenés, María

    2013-03-01

    "Death and its approach represent a field of study for nurses": thus ended his article Montserrat Salvador Borrell in Revista ROL de Enfermería in November 2010. Address it also from the point of view of the soul brings perspective and gives horizon in the sense of one greater understanding. Death, contemplated and lived exclusively from the material side, flees from as a not only traumatic event for which the experience but also unsurpassed for loved ones. Paradoxically, it's a more natural and frequent phenomenon. Why humans so hard that naturalness? Because we have not cultivated, sometimes not even recognized, other spiritual nature which forms us and who has the key to give sense to the decisive events. For many spiritual traditions, death is birth to another world that, from here, we call beyond. In accompaniment of soul seminars, based on the teachings transmitted by Elisabeth Kübler Ros and Marie Lise Labonte, patterns are learned to accompany the personality and soul--which does not always go according to the time of split--and to help the soul to rediscover his way toward the light, toward the origin. The soul knows it well, but the emotional ties forged during his earthly journey can retain it and hinder the process, which becomes slow and painful. The accompaniment of the soul is a "service" human, social and spiritual. Guides in a forgotten and often feared territory is a relief. You have guides to prepare the great journey allows, especially, make peace with himself with others I with the world. The Egyptians used the symbol of the solar boat, which waits for the deceased to cross the great waters. Nurses are, unknowingly, barqueras of small and large transits of existence. PMID:23654191

  18. Learning to sing with the accompaniment of the gusle in Serbia in the XXI century

    Lajić-Mihajlović Danka

    2012-01-01

    Full Text Available In this paper, learning to sing with the accompaniment of the gusle is reconsidered within the concept of the preservation of traditional folk music as an intangible cultural heritage, that is, as a specific form of music education. Having dealt with a traditional way of providing continuity in the practice of guslars` (i. e. gusle players - which is learning by observing and imitating more experienced family members or guslars from nearby surrounding - some crucial moments in the history of singing with the accompaniment of the gusle, which also influenced the process of transmitting this skill, are demonstrated. In recent times, singing with the accompaniment of the gusle has been mastered not only within family circles but also in associations of guslars, at private schools of music, and lately there has also been an opportunity to learn this skill at some state schools of music. Accordingly, interviews with the members of an Association of guslars association that is particularly distinguished by the fostering young guslars, and interviews with gusle teachers and pupils who attend schools of music were used as primary sources for this paper, together with the specific experience of the author as a president of the jury in a young guslars’ competition. Since the poetic content of the songs is primarily learnt from printed collections or audio recordings, future guslars in fact master the musical skills of playing and singing along the instrument, that is, the skill of the vocal interpretation of a chosen written poetic text. Concerning these, some programmes and methods are discussed, as well as their effects on young guslars’ competence, principally from the point of style and aesthetics. Attention is particularly drawn to flaws in the teaching concept of future gusle players at state schools of music. In conclusion, the necessity of applying ethnomusicological findings to activities concerning the preservation of an intangible cultural

  19. Accompanying conditions in patients with chronic spontaneous urticaria and urticarial vasculitis: Results of a retrospective study

    Sevgi Akarsu

    2015-03-01

    Full Text Available Background and Design: Chronic spontaneous urticaria (CSU, the most common form of chronic urticaria, is characterized by spontaneous wheals and/or angioedema lasting longer than six weeks. Urticarial vasculitis (UV is a small vessel vasculitis; but is also included in the various classification systems of chronic urticaria by some authors. The aim of our study was to evaluate the frequency of accompanying conditions, and to compare the demographic, clinical and laboratory features of patients with CSU and UV. Materials and Methods: In this study, the files of CSU (n=146 and UV (n=43 patients who had been hospitalized between January 2010-December 2013 were retrospectively reviewed in terms of demographic data, disease-specific history, accompanying diseases, medications and laboratory findings. Results: When comparing between the two groups for statistical differences, it was found that the frequencies of personal atopy and angioedema were significantly higher in patients with CSU; however, the patients with UV had significantly higher rates for female gender and the presence of systemic symptoms compared to those with CSU. It was determined that the mean values for erythrocyte sedimentation rate and C-reactive protein, as well as the rates of antinuclear antibody positivity and hypocomplementemia were significantly higher in the UV group, compared with those in the CSU group. Accompanying factors were determined as infections, medications, autoimmune diseases and malignities in order of frequency in both CSU and UV groups, but only the frequency of autoimmune diseases was significantly higher in patients with UV group. Conclusion: Although various authors have reported that a large number (up to 40-60% of patients with UV may present only with wheals (clinically indistinguishable from CSU; we assumed that it would be more appropriate to be considered UV as a priority in patients especially who had systemic symptom, autoimmune disease

  20. Neuroendocrine-immune and metabolic accompaniments of cholecystokinetic effects of balneotherapy on SPA Truskavets’

    Marfiyan, O M; Korolyshyn, T A; Barylyak, L G; Kovbasnyuk, M M; Yavors’kyi, O V; Zukow, W; Popovych, I L

    2015-01-01

    Marfiyan O M, Korolyshyn T A, Barylyak L G, Kovbasnyuk M M, Yavors’kyi O V, Zukow W, Popovych I L. Neuroendocrine-immune and metabolic accompaniments of cholecystokinetic effects of balneotherapy on SPA Truskavets’. Journal of Education, Health and Sport. 2015;5(5):21-30. ISSN 2391-8306. DOI: 10.5281/zenodo.17307 http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%285%29%3A21-30 https://pbn.nauka.gov.pl/works/556532 http://dx.doi.org/10.5281/zenodo.17307 For...

  1. X-ray microbeam quantification of grain subdivision accompanying large deformations of copper

    Polychromatic synchrotron x-ray microbeams offer a very efficient alternative to electron beam methods for quantifying the amount and character of grain subdivision accompanying large deformations. With a 0.01 mm diameter collimator, bending magnet radiation from a 3.0 GeV source and image storage plates, samples of copper with thicknesses greater than 0.1 mm have been studied. Results from an as-received sample and a sample deformed to 100% torsion are compared and illustrate how efficiently grain subdivision can be quantified with polychromatic microbeam diffraction

  2. Reverse U-shaped horseshoe kidney accompanied by gibbus deformity and spina bifida.

    Bozdogan, Erol; Demir, Mahmut; Konukoglu, Osman; Karakas, Ekrem

    2016-06-01

    Horseshoe kidney (HSK) is the most common fusion anomaly of kidneys. Diagnosis of horseshoe kidneys is made by the demonstration of an isthmus or band of renal tissue between the lower poles of the kidneys. Connection between the upper poles of the kidneys is extremely rare. Several types of skeletal anomalies can be seen concomitantly with HSK. In our present case, where the patient was a 3-year-old male, the connection of renal tissue was located between the upper poles of the kidneys. Furthermore, there was an accompanying spina bifida and Gibbus deformity secondary to anterior hypoplasia of the T10 vertebral body. PMID:27114084

  3. A typical case of hydrallantois accompanied by fetal monstrosity in a local ewe of Kashmir

    Hiranya Kumar Bhattacharyya

    2012-09-01

    Full Text Available A full termed local ewe with the history of continuous straining with labored breathing for last 24 hours was presented. The animal was disinclined to move with tense and round abdomen which developed rapidly during last two weeks. Caesarean section revealed hydrallantois accompanied by multiple fetal congenital abnormalities. The ewe was under observation for four weeks. Metritis developed 12 days post-operation and was treated successfully. The ewe was found active on 25 days post-surgery with gain of extra 3 kg bodyweight.

  4. A rare case of duodenal ulcer perforation accompanied by Boerhaave syndrome

    Rokicki, Wojciech; Rokicki, Marek

    2015-01-01

    Esophageal perforation is the fastest progressing and the most life-threatening disruption of gastrointestinal tract continuity. It must be regarded as an emergency condition that requires early diagnosis as well as very aggressive and rapid implementation of treatment in order to avoid serious complications and death. Methods of treatment for spontaneous esophageal perforation continue to be a matter of controversy. However, all authors emphasize that ultimate success depends largely on the time taken to establish the diagnosis. The authors of this study describe a rare case of duodenal ulcer perforation accompanied by Boerhaave syndrome. PMID:26702287

  5. The emission of atoms and molecules accompanying fracture of single-crystal MgO

    Dickinson, J. T.; Jensen, L. C.; Mckay, M. R.; Freund, F.

    1986-01-01

    The emission of particles due to deformation and fracture of materials has been investigated. The emission of electrons (exoelectron emission), ions, neutral species, photons (triboluminescence), as well as long wavelength electromagnetic radiation was observed; collectively these emissions are referred to as fractoemission. This paper describes measurements of the neutral emission accompanying the fracture of single-crystal MgO. Masses detected are tentatively assigned to the emission of H2, CH4, H2O, CO, O2, CO2, and atomic Mg. Other hydrocarbons are also observed. The time dependencies of some of these emissions relative to fracture are presented for two different loading conditions.

  6. Solutions manual to accompany Statistics and probability with applications for engineers and scientists

    Gupta, Bhisham C

    2013-01-01

    A solutions manual to accompany Statistics and Probability with Applications for Engineers and ScientistsUnique among books of this kind, Statistics and Probability with Applications for Engineers and Scientists covers descriptive statistics first, then goes on to discuss the fundamentals of probability theory. Along with case studies, examples, and real-world data sets, the book incorporates clear instructions on how to use the statistical packages Minitab® and Microsoft® Office Excel® to analyze various data sets. The book also features: Detailed discussions on sampling distributions, stati

  7. Mice lacking desmocollin 1 show epidermal fragility accompanied by barrier defects and abnormal differentiation

    Chidgey, M; Brakebusch, C; Gustafsson, E;

    2001-01-01

    epidermis because environmental insults are more stringent and wound healing is less rapid than in neonatal mice. This dermatitis is accompanied by localized hair loss associated with formation of utriculi and dermal cysts, denoting hair follicle degeneration. Possible resemblance of the lesions to human......The desmosomal cadherin desmocollin (Dsc)1 is expressed in upper epidermis where strong adhesion is required. To investigate its role in vivo, we have genetically engineered mice with a targeted disruption in the Dsc1 gene. Soon after birth, null mice exhibit flaky skin and a striking punctate...

  8. Solutions manual to accompany Combinatorial reasoning an introduction to the art of counting

    DeTemple, Duane

    2014-01-01

    This is a solutions manual to accompany Combinatorial Reasoning: An Introduction to the Art of CountingWritten by well-known scholars in the field, Combinatorial Reasoning: An Introduction to the Art of Counting introduces combinatorics alongside modern techniques, showcases the interdisciplinary aspects of the topic, and illustrates how to problem solve with a multitude of exercises throughout. The authors'' approach is very reader-friendly and avoids the ""scholarly tone"" found in many books on this topic.  

  9. Solutions manual to accompany Classical geometry Euclidean, transformational, inversive, and projective

    Leonard, I E; Liu, A C F; Tokarsky, G W

    2014-01-01

    Solutions Manual to accompany Classical Geometry: Euclidean, Transformational, Inversive, and Projective Written by well-known mathematical problem solvers, Classical Geometry: Euclidean, Transformational, Inversive, and Projective features up-to-date and applicable coverage of the wide spectrum of geometry and aids readers in learning the art of logical reasoning, modeling, and proof. With its reader-friendly approach, this undergraduate text features self-contained topical coverage and provides a large selection of solved exercises to aid in reader comprehension. Material in this text can be tailored for a one-, two-, or three-semester sequence.

  10. Observation of lithium isotope effect accompanying electrochemical insertion of lithium into zinc

    Lithium was electrochemically inserted into zinc from the mixed solution of ethylene carbonate and methyl ethyl carbonate containing 1 M LiClO4, and the lithium isotope effect accompanying the insertion was investigated. The x value of LixZn after the electrolysis ranged from 0.013 to 0.139. The single-stage separation factor, S, ranged from 1.005 to 1.023 with the lighter isotope, 6Li, being preferentially fractionated into the zinc phase. The present results on lithium isotope effect qualitatively agreed with calculated results based on the molecular orbital theory. (author)

  11. The Asian countries and the non-proliferation treaty prorogation

    This work deals with the non-proliferation treaty prorogation of Asia. The position of the asian countries under the old non-proliferation treaty is given. It includes the 1968 non-proliferation treaty signatories, the calling in question again and the criticisms revealed by the asian countries. The positions and the open forecasts expressed on the non-proliferation treaty prorogation and the article on the elimination of the nuclear weapons are also given. (O.L.)

  12. Proliferation of osteoblast cells on nanotubes

    F.WATARI; T.AKASAKA; Xiaoming LI; M.UO; A.YOKOYAMA

    2009-01-01

    Carbon nanotubes (CNT) have a unique structme and feature. In the present study, cell proliferation was performed on the scaffolds of single-walled CNTs (SWCNT), multiwalled CNTs (MWCNT), and on gra-phita, one of the representative isomorphs of pure carbon,for the sake of comparison. Scanning electron microscopy observation of the growth of osteoblast-like cells (Saps2) cultttred on CNTs showed the morphology fully developed for the whole direction, which is different from that extended to one direction on the usual scaffold. Numerous filopodia were grown from cell edge, extended far long and combined with the CNT meshwork. CNTs showed the affinity for collagen and proteins. Proliferated cell numbers are largest on SWCNTs, followed by MWCNTs, and are very low on graphite. This is in good agreement with the sequence in the results of the adsorbed amount of proteins and expression of alkaline phosphatase activity for these scaffolds. The adsorption of protains would be one of the most influential factors to make a contrast difference in cell attachment and proliferation between graphite and CNTs,both of which are isomorphs of carbon and composed of similar graphene sheet crystal structure. In addition, the nanosize meshwork structure with large porosity is another properly responsible for the excellent cell adhesion and growth on CNTs. CNTs could be the favorable materials for biomedical applications.CNTs with different structures and compositions have been synthesized and discovered [3]. Nanomaterials [2-9] and nanocomposites [10-15] may have various effects onliving organisms. In this study, a fundamental study for biomedical application, cell proliferation was performed on various nanotubes (biT), including (1) single-walled CNTs (SWCNT), (2) multiwalled CNTs (MWCNT), and on graphite, an isomorph of CNT, as a comparison.Figure 1 shows the schematic figures of two different crystal structures of carbon: graphite and CNT. Graphite has the layer-by-layer laminated

  13. Effect of recombinant porcine IGF-binding protein-3 on proliferation of embryonic porcine myogenic cell cultures in the presence and absence of IGF-I.

    Pampusch, M S; Kamanga-Sollo, E; White, M E; Hathaway, M R; Dayton, W R

    2003-02-01

    IGF-binding protein (IGFBP)-3 is produced by cultured porcine embryonic myogenic cell (PEMC) cultures and is secreted into the medium. Levels of secreted IGFBP-3 and IGFBP-3 mRNA are significantly reduced during differentiation and increase after differentiation is complete, suggesting that IGFBP-3 may play some role in myogenesis and/or in changes in myogenic cell proliferation that accompany differentiation. IGFBP-3 reportedly may either suppress or stimulate proliferation of cultured cells depending on cell type. Additionally, IGFBP-3 has been shown to affect proliferation via both IGF-dependent and IGF-independent mechanisms in some cell types but not all. Currently, the effect, if any, of IGFBP-3 on myogenic cell proliferation is not known. Consequently, the goal of this study was to assess the IGF-I-dependent and IGF-I-independent actions of recombinant porcine IGFBP-3 on proliferation of cultured porcine myogenic cells. To facilitate these investigations, we have expressed porcine IGFBP-3 in the baculovirus system, purified and characterized the expressed recombinant porcine IGFBP-3 (rpIGFBP-3), and produced and characterized an anti-porcine IGFBP-3 antibody that neutralizes the biological activity of porcine IGFBP-3. rpIGFBP-3 suppressed IGF-I-stimulated proliferation of PEMCs in a concentration-dependent manner with equimolar concentrations of IGF-I and rpIGFBP-3, resulting in complete suppression of IGF-I-stimulated proliferation. rpIGFBP-3 also suppressed Long-R3-IGF-I-stimulated proliferation of PEMC, indicating that rpIGFBP-3 possesses IGF-independent activity in this cell system. These data have established that IGFBP-3 has the potential to affect proliferation of PEMCs during critical periods of muscle development that may impact ultimate muscle mass achievable postnatally. PMID:12553871

  14. An infantile case of cytomegalovirus induced idiopathic thrombocytopenic purpura with predominant proliferation of CD10 positive lymphoblast in bone marrow.

    Mizutani, K; Azuma, E; Komada, Y; Ito, M; Sakurai, M; Hironaka, T; Hirai, K

    1995-02-01

    An infant with cytomegalovirus infection (CMV) developed idiopathic thrombocytopenic purpura (ITP) at 4 months of age. A bone marrow (BM) aspiration showed a remarkable increase of immature megakaryocytes and prominent proliferation of lymphoblasts. Flow cytometric analysis of the bone marrow cells showed that the predominant cells in the lymphocyte cluster were of B-lineage (CD19) with CD10 (common acute lymphoblastic leukemia antigen) positive. Virus study showed a higher titer of CMV antibody. Cytomegalovirus DNA was detected by the polymerase chain reaction (PCR) method in urine, peripheral cells and marrow cells. Low-grade fever, diarrhea and petechiae were accompanied by mild liver dysfunction. Complete remission was made with intravenous high-dose immunoglobulin (IVIg) without progression to overt acute leukemia. The percentage of CD10+/CD19+ lymphocytes in bone marrow also diminished. We postulated that the proliferation of immature lymphocytes and megakaryocytes in bone marrow was caused by maturation arrest that might result from CMV infection. PMID:7754772

  15. Proliferation resistance design of a plutonium cycle (Proliferation Resistance Engineering Program: PREP)

    Sorenson, R.J.; Roberts, F.P.; Clark, R.G.

    1979-01-19

    This document describes the proliferation resistance engineering concepts developed to counter the threat of proliferation of nuclear weapons in an International Fuel Service Center (IFSC). The basic elements of an International Fuel Service Center are described. Possible methods for resisting proliferation such as processing alternatives, close-coupling of facilities, process equipment layout, maintenance philosophy, process control, and process monitoring are discussed. Political and institutional issues in providing proliferation resistance for an International Fuel Service Center are analyzed. The conclusions drawn are (1) use-denial can provide time for international response in the event of a host nation takeover. Passive use-denial is more acceptable than active use-denial, and acceptability of active-denial concepts is highly dependent on sovereignty, energy dependence and economic considerations; (2) multinational presence can enhance proliferation resistance; and (3) use-denial must be nonprejudicial with balanced interests for governments and/or private corporations being served. Comparisons between an IFSC as a national facility, an IFSC with minimum multinational effect, and an IFSC with maximum multinational effect show incremental design costs to be less than 2% of total cost of the baseline non-PRE concept facility. The total equipment acquisition cost increment is estimated to be less than 2% of total baseline facility costs. Personnel costs are estimated to increase by less than 10% due to maximum international presence. 46 figures, 9 tables.

  16. Proliferation resistance design of a plutonium cycle (Proliferation Resistance Engineering Program: PREP)

    This document describes the proliferation resistance engineering concepts developed to counter the threat of proliferation of nuclear weapons in an International Fuel Service Center (IFSC). The basic elements of an International Fuel Service Center are described. Possible methods for resisting proliferation such as processing alternatives, close-coupling of facilities, process equipment layout, maintenance philosophy, process control, and process monitoring are discussed. Political and institutional issues in providing proliferation resistance for an International Fuel Service Center are analyzed. The conclusions drawn are (1) use-denial can provide time for international response in the event of a host nation takeover. Passive use-denial is more acceptable than active use-denial, and acceptability of active-denial concepts is highly dependent on sovereignty, energy dependence and economic considerations; (2) multinational presence can enhance proliferation resistance; and (3) use-denial must be nonprejudicial with balanced interests for governments and/or private corporations being served. Comparisons between an IFSC as a national facility, an IFSC with minimum multinational effect, and an IFSC with maximum multinational effect show incremental design costs to be less than 2% of total cost of the baseline non-PRE concept facility. The total equipment acquisition cost increment is estimated to be less than 2% of total baseline facility costs. Personnel costs are estimated to increase by less than 10% due to maximum international presence. 46 figures, 9 tables

  17. Increased susceptibility to fungal disease accompanies adaptation to drought in Brassica rapa.

    O'Hara, Niamh B; Rest, Joshua S; Franks, Steven J

    2016-01-01

    Recent studies have demonstrated adaptive evolutionary responses to climate change, but little is known about how these responses may influence ecological interactions with other organisms, including natural enemies. We used a resurrection experiment in the greenhouse to examine the effect of evolutionary responses to drought on the susceptibility of Brassica rapa plants to a fungal pathogen, Alternaria brassicae. In agreement with previous studies in this population, we found an evolutionary shift to earlier flowering postdrought, which was previously shown to be adaptive. Here, we report the novel finding that postdrought descendant plants were also more susceptible to disease, indicating a rapid evolutionary shift to increased susceptibility. This was accompanied by an evolutionary shift to increased specific leaf area (thinner leaves) following drought. We found that flowering time and disease susceptibility displayed plastic responses to experimental drought treatments, but that this plasticity did not match the direction of evolution, indicating that plastic and evolutionary responses to changes in climate can be opposed. The observed evolutionary shift to increased disease susceptibility accompanying adaptation to drought provides evidence that even if populations can rapidly adapt in response to climate change, evolution in other traits may have ecological effects that could make species more vulnerable. PMID:26648585

  18. The Harmonic Walk: An Interactive Physical Environment to Learn Tonal Melody Accompaniment

    Marcella Mandanici

    2016-01-01

    Full Text Available The Harmonic Walk is an interactive physical environment designed for learning and practicing the accompaniment of a tonal melody. Employing a highly innovative multimedia system, the application offers to the user the possibility of getting in touch with some fundamental tonal music features in a very simple and readily available way. Notwithstanding tonal music is very common in our lives, unskilled people as well as music students and even professionals are scarcely conscious of what these features actually are. The Harmonic Walk, through the body movement in space, can provide all these users a live experience of tonal melody structure, chords progressions, melody accompaniment, and improvisation. Enactive knowledge and embodied cognition allow the user to build an inner map of these musical features, which can be acted by moving on the active surface with a simple step. Thorough assessment tests with musicians and nonmusicians high school students could prove the high communicative power and efficiency of the Harmonic Walk application both in improving musical knowledge and in accomplishing complex musical tasks.

  19. Influence of accompanying substances of hemp fibres on their electric resistance

    Pejić Biljana

    2006-01-01

    Full Text Available Hemp fibres belong to the group of natural, cellulose bast fibres. These fibres have exceptional properties such as: antimicrobial effect, absence of allergy effect, extraordinary sorption properties, good electro-physical properties (small static electricity in regard to other cellulose fibres as well as high values of breaking strength (the natural fibre with the highest strength. However, hemp fibres have some defects: heterogeneous chemical composition, large quantity of accompanying substances (lignin pectins, waxes and unsatisfactory fineness and eveness. It is possible to a great extent to eliminate or reduce, the defects of hemp fibres by of appropriate modification treatments. In order to determine the appropriate modification treatment of hemp fibres, the dependences between the chemical composition, fineness and electric resistance of hemp fibres were presented in this paper. In the experimental part of the paper, by the application of a procedure for the determination of the chemical composition, the accompanying supstances of hemp fibres were gradually removed. After each phase some fibrous substrates were separated. After that the fineness and electric resistance were determined. This experiment was conducted in order to define the influence of each component of hemp fibres on the fineness and electric resistance. In this paper, hemp fibres were modified by an aqueous solution of sodium hydroxide, under different conditions of modification. The influence of modification conditions on the fineness and electric resistance were studied.

  20. Analysis of Pathogen Distribution and Drug Resistance of Nosocomial Infections Accompanied in Patients with Malignant Tumor

    YAO Dong-mei; CAO Wei; QING Zhi-ju

    2008-01-01

    Objective:To investigate the pathogen distribution and drug resistance of nosocomial infections accompanied in patients with malignant tumor.Methods:The pathogen culture and drug-sensitivity data of 107 specimens isolated from malignant tumor patients accompanied with nosocomial infection were retrospectively analyzed.Results:Among 118 strains of pathogens isolated from 107 specimens,77 were gram-negative bacillus(65.3%),26 were gram-positive coccus(65.3%),and 15 were fungus(12.7%).Eleven specimens were revealed to have combined infection of bacterium and fungus.Gram-negative bacillus showed high sensitivity to amikacin,ciprofloxacin,and tienam.Gram-positive cocci were highly sensitive to tienam and vancomycin.The bacteria were resistant to other antibiotics in different degrees.Vancomycin-resistant staphylococcus was not detected.Candida was sensitive to antifungals.Conclusion:Conditional pathogenic bacteria were mainly responsible for nosocomial infections in malignant tumor patients with considerable drug resistance.This shows that bacterial tests and the rational use of antibiotics should be emphasized in clinical practice to prevent the formation of drug resistant strains and further endogenous infections.

  1. The contribution of perceived parental and familial characteristics to attitudes toward accompanied driving among young drivers.

    Taubman-Ben-Ari, Orit

    2011-09-01

    One of the main requirements of the graduated driving licensing system in Israel is that new drivers be accompanied by an experienced driver (usually one of their parents) for the first three months after receipt of their license. The current series of studies examined the associations between young drivers' attitudes toward accompanied driving (ATAD) and their perception of their parents' driving styles and parenting modes, as well as dynamic of their family. Young drivers completed questionnaires assessing their ATAD, and either perceived parental driving style (Study 1, n=100), perceived parenting style (Study 2, n=120), or perceived family dynamic (Study 3, n=254). The results indicated significant associations between the young drivers' ATAD of tension, relatedness, avoidance, disapproval, and anxiety, and their own perceptions of the characteristic driving styles of their parents (risky, anxious, angry, and careful). They also indicated significant relationship between youngsters' ATAD and their perceptions of their parents' parenting modes (responsive, demanding, and autonomy-granting). Finally, the results indicated significant negative associations between ATAD of tension, avoidance, disapproval, and anxiety, and family cohesion and adaptability, and a positive association between these two and relatedness. The results are discussed in respect to the relationship between the parental model and the young drivers' ATAD in particular, and risk management in general, and practical implications of these results are offered. PMID:21658499

  2. EFFECTS OF RETINOIC ACID ON PROLIFERATION AND DIFFERENTIATION OF A HUMAN OVARIAN CARCINOMA CELL LINE: 3AO

    Ming-Juan Xu; Ying Cui; Ning Hui; Yu-Jian Liu

    2005-01-01

    Objective To observe the effects ofretinoic acid (RA) on the proliferation and differentiation of a human ovarian carcinoma cell line: 3AO cells.Methods 3AO cell proliferation was evaluated by viable cell count, percentage of cells in each cycle phase were analyzed by flow cytometric analysis, alkaline phosphatase (AKP) activity was determined as described, and CA125 expression was measured by ELISA.Results RA could inhibit the proliferation of 3AO cells accompanied with morphological changes in a dose-dependent manner. Cell cycle analysis indicated that RA inhibition of 3AO cells growth occurred through induction of G1 arrest with a concomitant reduction in the proportion of cells in S phase, AKP activity increased significantly after treatment with RA (0. l μmol/L) for 1-5 days. Dose-response studies revealed that the AKP activity increased to a different extent as a function of RA concentrations. Furthermore, RA could suppress the expression of CA125 tumor marker in 3AO cells.Conclusion RA could markedly inhibit the proliferation and induce the differentiation of 3AO cells.

  3. N-Cadherin Induction by ECM Stiffness and FAK Overrides the Spreading Requirement for Proliferation of Vascular Smooth Muscle Cells

    Keeley L. Mui

    2015-03-01

    Full Text Available In contrast to the accepted pro-proliferative effect of cell-matrix adhesion, the proliferative effect of cadherin-mediated cell-cell adhesion remains unresolved. Here, we studied the effect of N-cadherin on cell proliferation in the vasculature. We show that N-cadherin is induced in smooth muscle cells (SMCs in response to vascular injury, an in vivo model of tissue stiffening and proliferation. Complementary experiments performed with deformable substrata demonstrated that stiffness-mediated activation of a focal adhesion kinase (FAK-p130Cas-Rac signaling pathway induces N-cadherin. Additionally, by culturing paired and unpaired SMCs on microfabricated adhesive islands of different areas, we found that N-cadherin relaxes the spreading requirement for SMC proliferation. In vivo SMC deletion of N-cadherin strongly reduced injury-induced cycling. Finally, SMC-specific deletion of FAK inhibited proliferation after vascular injury, and this was accompanied by reduced induction of N-cadherin. Thus, a stiffness- and FAK-dependent induction of N-cadherin connects cell-matrix to cell-cell adhesion and regulates the degree of cell spreading needed for cycling.

  4. Ghrelin inhibits proliferation and increases T-type Ca{sup 2+} channel expression in PC-3 human prostate carcinoma cells

    Diaz-Lezama, Nundehui; Hernandez-Elvira, Mariana [Laboratory of Neuroendocrinology, Institute of Physiology, Autonomous University of Puebla (BUAP), Puebla (Mexico); Sandoval, Alejandro [School of Medicine FES Iztacala, National Autonomous University of Mexico (UNAM), Tlalnepantla (Mexico); Monroy, Alma; Felix, Ricardo [Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), Mexico City (Mexico); Monjaraz, Eduardo, E-mail: emguzman@siu.buap.mx [Laboratory of Neuroendocrinology, Institute of Physiology, Autonomous University of Puebla (BUAP), Puebla (Mexico)

    2010-12-03

    Research highlights: {yields} Ghrelin decreases prostate carcinoma PC-3 cells proliferation. {yields} Ghrelin favors apoptosis in PC-3 cells. {yields} Ghrelin increase in intracellular free Ca{sup 2+} levels in PC-3 cells. {yields} Grelin up-regulates expression of T-type Ca{sup 2+} channels in PC-3 cells. {yields} PC-3 cells express T-channels of the Ca{sub V}3.1 and Ca{sub V}3.2 subtype. -- Abstract: Ghrelin is a multifunctional peptide hormone with roles in growth hormone release, food intake and cell proliferation. With ghrelin now recognized as important in neoplastic processes, the aim of this report is to present findings from a series of in vitro studies evaluating the cellular mechanisms involved in ghrelin regulation of proliferation in the PC-3 human prostate carcinoma cells. The results showed that ghrelin significantly decreased proliferation and induced apoptosis. Consistent with a role in apoptosis, an increase in intracellular free Ca{sup 2+} levels was observed in the ghrelin-treated cells, which was accompanied by up-regulated expression of T-type voltage-gated Ca{sup 2+} channels. Interestingly, T-channel antagonists were able to prevent the effects of ghrelin on cell proliferation. These results suggest that ghrelin inhibits proliferation and may promote apoptosis by regulating T-type Ca{sup 2+} channel expression.

  5. The non-proliferation role of the IAEA under the nuclear non-proliferation treaty

    The twenty-fifth anniversary of the Treaty on the Non-Proliferation of Nuclear Weapons ('NPT' or 'Treaty') is rapidly approaching, prompting a review of its operation, and of the role of the International Atomic Energy Agency ('IAEA' or 'Agency') in applying safeguards with a view to verifying compliance by States Parties with the non-proliferation undertakings of the Treaty. Some of the most significant challenges to the NPT, and to IAEA safeguards, have presented themselves in the four years since the last NPT Review Conference in 1990. The author will describe in general the IAEA's NPT-related safeguards activities as carried out over the past twenty-five years, including the on-going efforts to strengthen the safeguards system, and cast a forward look toward possible future tasks of the IAEA in connection with the international non-proliferation regime

  6. Dynamic Alterations to α-Actinin Accompanying Sarcomere Disassembly and Reassembly during Cardiomyocyte Mitosis.

    Xiaohu Fan

    Full Text Available Although mammals are thought to lose their capacity to regenerate heart muscle shortly after birth, embryonic and neonatal cardiomyocytes in mammals are hyperplastic. During proliferation these cells need to selectively disassemble their myofibrils for successful cytokinesis. The mechanism of sarcomere disassembly is, however, not understood. To study this, we performed a series of immunofluorescence studies of multiple sarcomeric proteins in proliferating neonatal rat ventricular myocytes and correlated these observations with biochemical changes at different cell cycle stages. During myocyte mitosis, α-actinin and titin were disassembled as early as prometaphase. α-actinin (representing the sarcomeric Z-disk disassembly precedes that of titin (M-line, suggesting that titin disassembly occurs secondary to the collapse of the Z-disk. Sarcomere disassembly was concurrent with the dissolution of the nuclear envelope. Inhibitors of several intracellular proteases could not block the disassembly of α-actinin or titin. There was a dramatic increase in both cytosolic (soluble and sarcomeric α-actinin during mitosis, and cytosolic α-actinin exhibited decreased phosphorylation compared to sarcomeric α-actinin. Inhibition of cyclin-dependent kinase 1 (CDK1 induced the quick reassembly of the sarcomere. Sarcomere dis- and re-assembly in cardiomyocyte mitosis is CDK1-dependent and features dynamic differential post-translational modifications of sarcomeric and cytosolic α-actinin.

  7. Dynamic Alterations to α-Actinin Accompanying Sarcomere Disassembly and Reassembly during Cardiomyocyte Mitosis

    Ali, Mohammad A. M.; Cho, Woo Jung; Lopez, Waleska; Schulz, Richard

    2015-01-01

    Although mammals are thought to lose their capacity to regenerate heart muscle shortly after birth, embryonic and neonatal cardiomyocytes in mammals are hyperplastic. During proliferation these cells need to selectively disassemble their myofibrils for successful cytokinesis. The mechanism of sarcomere disassembly is, however, not understood. To study this, we performed a series of immunofluorescence studies of multiple sarcomeric proteins in proliferating neonatal rat ventricular myocytes and correlated these observations with biochemical changes at different cell cycle stages. During myocyte mitosis, α-actinin and titin were disassembled as early as prometaphase. α-actinin (representing the sarcomeric Z-disk) disassembly precedes that of titin (M-line), suggesting that titin disassembly occurs secondary to the collapse of the Z-disk. Sarcomere disassembly was concurrent with the dissolution of the nuclear envelope. Inhibitors of several intracellular proteases could not block the disassembly of α-actinin or titin. There was a dramatic increase in both cytosolic (soluble) and sarcomeric α-actinin during mitosis, and cytosolic α-actinin exhibited decreased phosphorylation compared to sarcomeric α-actinin. Inhibition of cyclin-dependent kinase 1 (CDK1) induced the quick reassembly of the sarcomere. Sarcomere dis- and re-assembly in cardiomyocyte mitosis is CDK1-dependent and features dynamic differential post-translational modifications of sarcomeric and cytosolic α-actinin. PMID:26076379

  8. Position paper on nuclear proliferation issues preventing nuclear proliferation. A duty for the nuclear community

    The production of electricity from nuclear power plants is widely seen today as having an increasing role to play in meeting global energy requirements in a sustainable manner. Conscious of the inherently sensitive nature of nuclear technology and materials the ENS-HSC (European Nuclear Society - High Scientific Council) is well aware that a severe safety, security, environmental or proliferation mishap stemming from nuclear energy anywhere in the world would undermine the potential for nuclear energy to contribute to the global energy supply and the minimization of harmful carbon emissions. While the safety of nuclear power plants has continuously improved over the last three decades, the same degree of success cannot be claimed when it comes to the achievements of the international community in stemming the risk of nuclear weapons proliferation. This unfortunate situation is due to both technical and political reasons. The European nuclear industry is committed to the exclusively peaceful use of nuclear energy and to export nuclear facilities and related materials, equipment and technology solely in accordance with relevant national export laws and regulations, Nuclear Suppliers Group guidelines and pertinent United Nations Security Council Resolutions. The ENS-HSC considers that, as a manifestation of their strong commitment to nonproliferation, it is important for the nuclear industry to pay special attention to and promote proliferation-resistant designs and to take IAEA safeguards requirements into account at the design stage. Preventing nuclear proliferation is primarily the responsibility of states but, as major stakeholders, the nuclear industry and scientific community should actively support nuclear disarmament as foreseen in the Non-Proliferation Treaty and measures necessary to strengthen the non-proliferation regime, particularly the international control of the flux of nuclear material and technology. (orig.)

  9. The non-proliferation regime, vertical proliferation and the interests of the Federal Republic of Germany

    The disarmament orientation of the NPT, which stands beside the central aim of avoiding horizontal proliferation, raises a question: Does a compatibility exist between the non-proliferation policy of the Federal Republic and its security policy, which has its basic pillar in the nuclear deterrence strategy? Critics of this deterrence policy therefore, hinting to the disarmament determination of the NPT, demand that the Federal Republic should exercise its influence for the conclusion of a 'Comprehensive Test Ban Treaty' (CTBT), the establishment of a 'Nuclear-Weapons-Free-Zones' (NWFZ) in Europe, a 'No First Use'-Treaty (NFU) and finally the abolishment of all atomic weapons ('Zero Solution'). According to them such disarmament 'remedies' can reestablish or assure the waning or damaged international consensus for horizontal non-proliferation. This is a contribution for the establishment of a stable world order and will smooth the way for a prolongation of the NPT in the year 1995. An analysis of the history and the structure of interests shows that the policy of the Federal Republic of Germany is deeply rooted in the NPT and that a prolongation of the treaty and its own membership is a substantial object of the foreign and security policy. Consequently the Federal Republic has to face the demands for an intensification of 'anti-nuclear measures' and has to examine their acceptability and their usefulness with respect to non-proliferation. The structure of the problem encloses the following aspects: The security conception of the Federal Republic with its military-strategic essence; the provisions in article VI NPT for negotiations with the object of a world free of atomic weapons; the derived disarmament 'remedies' for strengthening the consensus for horizontal non-proliferation and, finally, the real interface between horizontal and vertical proliferation. (orig./DG)

  10. Nuclear energy and proliferation: A longer perspective

    Nuclear power has expanded slowly; and it is largely based on reactors that require no recycle. The original basis for concern about proliferation - that nuclear power would be used very widely and that it would be based on systems that required reprocessing - has been shaken. The present world nuclear energy system, which is small and based on no-recycle reactors, is relatively resistant to proliferation via diversion from power reactors. Though worry about proliferation can never be eliminated, the perceived connection between power and bombs can be slowly reduced. The proposal to link fuel take-back with waste disposal poses a dilemma for the ''nuclear environmental'' activists. This group objects to nuclear power because, in their view, reactor wastes threaten the environment, and because bombs and reactors are connected. Both objections are held, often passionately; it would be difficult to assess which takes primacy. This proposal further breaks the ''Nuclear Connection,'' but at the expense of adding a little to the volume of wastes the United States would have to dispose of. We are in effect saying that the loosening of the nuclear connection through the take-back scheme should take precedence over the tiny environmental burden incurred by the addition of a few percent to the total wastes the U.S. already must dispose of. It is hoped that the nuclear environmental community will recognize this trade-off, and will help create the atmosphere of public understanding necessary if fuel take-back is to be accepted in the United States

  11. Proliferation prevention in the commercial fuel cycle

    This website contains the papers presented on November 17, 1998 during the session, ''Proliferation Prevention in the Commercial Fuel Cycle,'' at the American Nuclear Society meeting in Washington, DC. The abstracts are in a separate section; individual papers also contain the author's bio and e-mail address. In the session planning phase, it was suggested that the following questions and other relevant issues be addressed: * What are the difficulties and issues with defining and enforcing international standards for the physical protection of Pu and HEU (beyond the Convention on the Physical protection of Nuclear Material, which primarily addresses transportation)? * How do we (or can we) keep nuclear technology in general, and reprocessing and enrichment technologies in particular, from spreading to undesirable organizations (including governments), in light of Article IV of the NPT? Specifically, can we (should we) prevent the construction of light-water reactors in Iran; and should we support the construction of light-water reactors in North Korea? * Are there more proliferation-resistant fuel cycles that would be appropriate in developing countries? * Can the concept of ''nonproliferation credentials'' be defined in a useful way? * Is there historical evidence to indicate that reprocessing (or enrichment of HEU) in the US, Japan, or the EURATOM countries has impacted the acquisition (or attempted acquisition) of nuclear weapons by other nations or groups? * What is the impact of a fissile material cutoff treaty (FMCT) be on commercial nuclear fuel cycles? * Does MOX spent fuel present a greater proliferation risk than LEU spent fuel? Although the authors did not explicitly attempt to answer all these questions, they did enlighten us about a number of these and related issues

  12. The risk of nuclear weapons proliferation

    During the later years the risks of nuclear proliferation have again become a major topic of interest. This is primarily due to the acute problems caused by Iraq, North Korea, and the 3 new states of the former USSR, Ukraine, Kazakhstan and Belarus. Analysis shows that security problems and prestige are the two most important motives, when the risks of proliferation are considered. But motives are not enough. To produce nuclear weapons a number of technological requirements must also be fulfilled. The country must be able to produce almost pure fissile material, i.e. 235U or 239Pu. It must also be able to solve a number of metallurgical, explosive, ignition, physics and other problems. These are in particular non-trivial, if a implosion weapon is to be designed. A review is made of the nuclear facilities in a number of the countries which have been suggested as possible future nuclear weapons countries. In particular facilities which can produce almost pure fissile materials, 235U and 239Pu, are considered. The possibility of nuclear terrorists have often been discussed in the media. However, it seems very unlikely that even a major terrorist or mafia organization will be able to solve all the weapons design problems, even if they could steal the fissile material. It is finally discussed what can be done to reduce the risk of further nuclear proliferation. Political pressure can be brought to bear on countries outside the NPT to join it, but it can be counter-productive, and sometimes the countries that are able to exert such pressure, are not willing to do so for other political reasons. The problem of countries which are party to the NPT, but which are believed to acquire nuclear weapons capability in violation of the treaty, can be countered by unannounced inspections of non-declared facilities. However, such inspections can only be meaningfully performed if the necessary intelligence is available. (EG)

  13. A nuclear proliferation antigen in chronic lymphoproliferative disease

    To determine whether the proliferation rates of tumour cells, in chronic lymphoproliferative disorders may reflect disease activity and relate to prognosis, we studied the expression of Ki-67% (a nuclear proliferation marker) by alkaline phosphatase anti-alkaline phosphatase technique (APAAP), in peripheral blood and bone marrow mononuclear cells (separated on ficoll-hypaque) and in lymph-node biopsies, from patients with chronic lymphatic leukemia (Cll), chronic lymphatic leukemia/prolymphocytic leukemia (CLL/PLL), prolymphocytic leukemia (PLL) and non-hodgkin's lymphoma with leukemic plase. The proliferation rate was determined for these patients at presentation and again two months after therapy (to detect any change with therapy). We found that the highest rate of proliferation in each group was parallel to the degree of malignancy i.e. PLL showed higher proliferation than CLL/PLL, and CLL/PLL showed higher proliferation than CLL. In the NHL group the highest proliferation rate was found in the high high-grade NHL, followed by intermediate grade NHL then the low grade NHL. Lymph node biopsies also showed the same relation between proliferation rates and degree of malignancy. Bone marrow cells did not show a particular pattern probably due to interference from the erythroid element and contamination by peripheral blood. Ki-67% was compared to other proliferation markers serum B2 microglobulin and lactate dehydrogenase. It was found to be an independent marker of proliferation it is unaffected by hepatic, renal and gastrointestinal elements and thus its specificity for the tumour proliferation

  14. Proliferation resistance assessment of nuclear systems

    The first part of the present paper describes the basic assessment procedure that is adopted in the analysis of the three generic nuclear systems. Once-through, fast breeder, and thermal recycle systems are then treated in Sections II, III, and IV, respectively. In each of these sections, a reference system is examined, possible technical and institutional improvements are considered, and alternative system types are indicated. Section V then discusses the relative proliferation resistance of the three generic systems. Although this paper emphasizes the analysis and comparison of individual fuel cycle alternatives, Section V indicates briefly how these analyses then have to be considered in a broader context where systems coexist

  15. Non-proliferation and international safeguards

    Full text: In my view, drastic nuclear disarmament by nuclear weapon States could be coupled with universal commitment to non-proliferation by non-nuclear weapon States by 1995 when the extension of the NPT Will be discussed. The incentives and disincentives for making and stockpiling nuclear weapons are first of all in the political and security fields, (Global and regional detente reduce the incentive, With the cold war gone, the US and Russia are now agreeing on far-reaching cuts in their nuclear arsenals and at some point the other declared nuclear weapon States Will follow.In the regional fields, we have seen how Argentina and Brazil are about to commit themselves to exclusively peaceful uses of the atom through the Latin American Tlatelolco Treaty. And we have seen how South Africa has joined the NPT. A new wave of States adhering to the NPT may be expected from countries in the former Soviet Union. Some have already come, others are on the way. Detente in the Middle East and on the Indian subcontinent would improve the outlook for non-proliferation in these areas. A second barrier to nuclear proliferation lies in export restrictions on sensitive nuclear material and equipment, Following the discoveries in Iraq, these restrictions are being strengthened in a large number of States. A third barrier to nuclear proliferation lies in the economic and political consequences that would follow for a State if IAEA safeguards inspection revealed activities aimed at the production of nuclear weapons. These must have a high degree of reliability. The case of Iraq showed that it was possible for a closed, highly militarized State to hide nuclear activities from the IAEA and the world We are now drawing the lessons from this case. It is not physically possible for inspectors to look into every building and basement in vast countries, They must have information about where to look, and the IAEA is significantly strengthening its information basis. The IAEA has also re

  16. Nuclear power and the proliferation issue

    This lecture addresses the question of ''accessibility'' of plutonium as it would arise in fully commercial LMFBR and LWR once-through operations. It is pointed out that the storage of spent LWR fuel results in the progressive accumulation of plutonium which becomes increasingly accessible with time (the ''plutonium mine'') whereas with the LMFBR, economic considerations demand the rapid recycle of plutonium. Furthermore, the fast breeder reactor is primarily a plutonium incinerator, the net production of plutonium being rather small and controllable so as not to exceed society's needs for new energy supplies. An eight-point world nuclear development programme is suggested, taking full note of non-proliferation considerations

  17. Good news, bad news on proliferation

    While Argentina and Brazil now seem less likely to acquire nuclear weapons, Indian and Pakistani intentions remain uncertain. The Israeli nuclear program and recent allegations of black-marketing are even more disturbing. The author notes the positive developments in Latin America and some hopeful signs in South Asia, despite uncertainties over their final outcome. He finds Israel's program the most disturbing because of the deployment of Jericho II missiles and indications that Israel possesses a fully militarized nuclear force which was developed by illegal means. These activities could politicize the Non-Proliferation Treaty review conference. 14 references

  18. Bizzarre parosteal osteochondromatous proliferation: case report.

    Onesti, Maria Giuseppina; Carella, Sara; Amorosi, Vittoria; Pedace, Doria; Campagna, Domenico; Fino, Pasquale; Latini, Claudio

    2013-01-01

    Nora's tumor, also known as bizzarre parosteal osteochondromatous proliferation (POPB), is an exophytic outgrowth arising from the cortical surface of the bone that consists of a mixture of bone, cartilage and fibrous tissue. It is a benign lesion with atipical microscopic features and a tendency to recur. It must be distinguishable from parosteal osteogenic sarcoma, parosteal chondrosarcoma, osteochondroma, florid reactive periostitis, turret exostosis, subungueal exostosis, myositis ossificans. The treatment is surgical, but a high rate of local relapse is described. The diagnosis is histological. We report a case of a patient with POPB involving the foot, underwent surgical excision and with no evidence of recurrence at one year. PMID:23648846

  19. Sensor Fusion for Nuclear Proliferation Activity Monitoring

    Adel Ghanem, Ph D

    2007-03-30

    The objective of Phase 1 of this STTR project is to demonstrate a Proof-of-Concept (PoC) of the Geo-Rad system that integrates a location-aware SmartTag (made by ZonTrak) and a radiation detector (developed by LLNL). It also includes the ability to transmit the collected radiation data and location information to the ZonTrak server (ZonService). The collected data is further transmitted to a central server at LLNL (the Fusion Server) to be processed in conjunction with overhead imagery to generate location estimates of nuclear proliferation and radiation sources.

  20. Director`s series on proliferation

    Bailey, K.C.; Price, M.E. [eds.

    1994-08-12

    This fifth edition contains some of the papers that were presented in July 1994 at the Lawrence Livermore National conference entitled ``NPT: Review and Extension.`` Topics covered include: strategic warning and new nuclear states, the future for nuclear weapons, possibly stopping North Korean nukes without a war, Article VI of the nuclear non-proliferation treaty from the Chinese perspective, Article VI issues, Article VI and other NPT issues form the perspective of Russia, NPT review and extension, and finally problems facing total nuclear disarmament.

  1. Nuclear non-proliferation: Revisiting the basics

    The 1970 Treaty on the Non- Proliferation of Nuclear Weapons (NPT), with 188 States party, represents the cornerstone of the global nuclear non-proliferation and disarmament effort -- and despite recent challenges, it has never been more relevant than it is today. But if we are to move forward, it is essential to revisit a number of the basic assumptions and features of the existing regime, and consider a number of new approaches. A key assumption at the core of the NPT was that the asymmetry between nuclear-weapon States and non-nuclear-weapon States would gradually disappear. Progress has been made on the nuclear disarmament front, but much more remains to be done. Nuclear disarmament strategies have for the most part focused on the negotiation of bilateral nuclear arms control agreements between the holders of the two largest nuclear arsenals, and a few multilateral agreements designed to curb the quantitative and qualitative development of nuclear weapons. With the end of the Cold War as an impetus, some progress was made in the early and mid-1990s, but the process unfortunately slowed in the latter part of the decade. The discoveries of a clandestine nuclear weapon programme in Iraq after the 1991 Gulf War made it painfully clear that the IAEA verification system, with its focus on declared nuclear activities and its limited rights of access to information and sites, was not adequate for the IAEA to provide the comprehensive peaceful use assurances required under the NPT. This stark realization prompted the international community to significantly expand the IAEA's verification rights. These new rights were incorporated into a 1997 protocol additional to safeguards agreements, with a request for all States to subscribe to it. For the IAEA to provide the required assurances, it must have the required authority. It is clear that the success of the IAEA verification regime will depend heavily on achieving universal adherence to the required safeguards agreements

  2. Utility of Social Modeling for Proliferation Assessment - Preliminary Findings

    Often the methodologies for assessing proliferation risk are focused around the inherent vulnerability of nuclear energy systems and associated safeguards. For example an accepted approach involves ways to measure the intrinsic and extrinsic barriers to potential proliferation. This paper describes preliminary investigation into non-traditional use of social and cultural information to improve proliferation assessment and advance the approach to assessing nuclear material diversion. Proliferation resistance assessment, safeguard assessments and related studies typically create technical information about the vulnerability of a nuclear energy system to diversion of nuclear material. The purpose of this research project is to find ways to integrate social information with technical information by explicitly considering the role of culture, groups and/or individuals to factors that impact the possibility of proliferation. When final, this work is expected to describe and demonstrate the utility of social science modeling in proliferation and proliferation risk assessments.

  3. Transition from Preinvasive Carcinoma In Situ to Seminoma Is Accompanied by a Reduction of Connexin 43 Expression in Sertoli Cells and Germ Cells

    Ralph Brehm

    2006-06-01

    Full Text Available Carcinoma in situ (CIS represents the preinvasive stage of human germ cell tumors, but the mechanism leading to pubertal proliferation and invasive malignancy remains unknown. Among testicular gap functional proteins, connexin 43 (Cx43 represents the predominant Cx, and, previously, an inverse correlation between synthesis of Cx43 protein and progression of tumor development was detected. In the present study, using cDNA microarray analysis, in situ hybridization, semi quantitative reverse transcription-polymerase chain reaction (RT-PCR from tissue homogenates, RT-PCR from microdissected tubules with normal spermatogenesis and CIS, and seminoma cells from invasive seminoma, we asked whether reduction of Cx43 protein is accompanied by a change of Cx43 transcripts. We detected a significant downregulation of Cx43 at mRNA level in Sertoli and germ cells starting in seminiferous tubules infiltrated with CIS and resulting in a complete loss in seminoma cells. It was demonstrated that downregulation of Cx43 expression in neoplastic human testis takes place at the transcriptional level and starts in CIS. This reduction of Cx43 expression further suggests that early intratubular derangement in Cx43 gene expression and disruption of intercellular communication between Sertoli cells and/or Sertoli and preinvasive tumor cells may play a role in the progression phase of human seminoma development.

  4. Loss of Dok-1 and Dok-2 in mice causes severe experimental colitis accompanied by reduced expression of IL-17A and IL-22.

    Waseda, Masazumi; Arimura, Sumimasa; Shimura, Eri; Nakae, Susumu; Yamanashi, Yuji

    2016-09-01

    Appropriate immune responses and mucosal barrier functions are required for the maintenance of intestinal homeostasis. Defects in this defense system may lead to inflammatory disorders such as inflammatory bowel disease. Downstream of tyrosine kinases 1 (Dok-1) and its closest homolog, Dok-2, are preferentially expressed in immune cells, and play essential roles in the negative regulation of multiple signaling pathways in both innate and adaptive immunity. However, the function of these proteins in intestinal homeostasis remained unclear. Here we show that Dok-1/-2 double knockout (DKO) mice were highly susceptible to dextran sodium sulfate (DSS)-induced colitis compared with Dok-1 or Dok-2 single KO and wild type (WT) mice. Furthermore, DSS-treated Dok-1/-2 DKO mice exhibited increased colonic tissue damage accompanied by reduced proliferation of the epithelial cells relative to WT controls, suggesting that Dok-1/-2 DKO mice have defects in the repair of intestinal epithelial lesions. In addition, the levels of the Th17 cytokines IL-17A and IL-22, which have protective roles in DSS-induced colitis, were reduced in DSS-treated Dok-1/-2 DKO mice compared with WT mice. Taken together, our results demonstrate that Dok-1 and Dok-2 negatively regulate intestinal inflammation, apparently through the induction of IL-17A and IL-22 expression. PMID:27450811

  5. Identification of genetic networks involved in the cell injury accompanying endoplasmic reticulum stress induced by bisphenol A in testicular Sertoli cells

    To identify detailed mechanisms by which bisphenol A (BPA), an endocrine-disrupting chemical, induces cell injury in mouse testicular Sertoli TTE3 cells, we performed genome-wide microarray and computational gene network analyses. BPA (200 μM) significantly decreased cell viability and simultaneously induced an increase in mRNA levels of HSPA5 and DDIT3, endoplasmic reticulum (ER) stress marker genes. Of the 22,690 probe sets analyzed, BPA down-regulated 661 probe sets and up-regulated 604 probe sets by >2.0-fold. Hierarchical cluster analysis demonstrated nine gene clusters. In decreased gene clusters, two significant genetic networks were associated with cell growth and proliferation and the cell cycle. In increased gene clusters, two significant genetic networks including many basic-region leucine zipper transcription factors were associated with cell death and DNA replication, recombination, and repair. The present results will provide additional novel insights into the detailed molecular mechanisms of cell injury accompanying ER stress induced by BPA in Sertoli cells

  6. Comprehensive evaluation of functional and anatomical disorders of the patients with distal occlusion and accompanying obstructive sleep apnea syndrome

    Nabiev, F. H.; Dobrodeev, A. S.; Libin, P. V.; Kotov, I. I.; Ovsyannikov, A. G.

    2015-11-01

    The paper defines the therapeutic and rehabilitation approach to the patients with Angle's classification Class II dento-facial anomalies, accompanied by obstructive sleep apnea (OSA). The proposed comprehensive approach to the diagnostics and treatment of patients with posterior occlusion, accompanied by OSA, allows for objective evaluation of intensity of a dento-facial anomaly and accompanying respiratory disorders in the nasal and oral pharynx, which allows for the pathophysiological mechanisms of OSA to be identified, and an optimal plan for surgical procedures to be developed. The proposed comprehensive approach to the diagnostics and treatment of patients with Angle's classification Class II dento-facial anomalies provides high functional and aesthetic results.

  7. Visuomotor skill learning and accompanying changes in corticospinal excitability: Causal relation or epiphenomena?

    Christiansen, Lasse; Bojsen-Møller, Emil; Madsen, Mads Alexander Just; Thomas, Richard; Nielsen, Jens Bo; Lundbye-Jensen, Jesper

    Background: A single session of motor skill learning (MSL) is accompanied by a transient increase in corticospinal excitability (CSE)[1]. A similar increase in CSE can be induced by pairing peripheral electrical and transcranial magnetic stimulations (PAS)[2]. The behavioural and neurophysiological...... (24 hours after day 2) consisted of a delayed retention test of the motor skill. The area under the recruitment curve (AURC), the plateau (MEPmax) and the slope of the RC were used as measurements for CSE. Changes in these parameters associated with PAS and MSL were compared within each subject to....... No relation between changes in CSE and performance was observed. The PAS protocol resulted in similar increases in CSE at both time points. However, there was no relation between changes in CSE following MSL and PAS for any of the investigated parameters. Discussion The present findings emphasize the...

  8. Learning to let go. Education on end-of-life accompaniment

    Mario Mapelli

    2014-12-01

    Full Text Available In the age we live in, death seems to be the last taboo. It’s surrounded by an embarrassed silence. The emotional and existential echoes provoked by death are frequently reduced within an interpretation of the phenomenon to a simple biological fact. The growing number of people finding themselves hospitalized in the final period of their life cannot be considered as a mere request for material care. Ill people and their relatives are involved in a process of interpreting their painful experience. For this reason, it is important to provide a context where feelings of grief, angst and fear can be authentically heard. Narrative education allows healthcare professionals to practice an interior dialogue, leading them to recognize the emotional substratum of care relationship and the meaning of accompanying people at the end of their life.

  9. Cannon Fire Soon to Accompany Bugle Call at Fort Detrick | Poster

    Beginning June 14, the familiar bugle calls at Fort Detrick will be joined by a special percussion instrument: a cannon. A single cannon shot will be fired on the first note of “Reveille,” which signals the start of each day and is accompanied by the raising of the American flag. “Reveille” sounds at 6:30 a.m. At 5 p.m., Fort Detrick plays “Retreat,” which alerts the post that the flag is about to be lowered. The cannon will fire on the last note of “Retreat.” After a five-second pause, “To the Color” plays as the flag is lowered.

  10. First-Order Structural Change Accompanied by Yb Valence Transition in YbInCu4

    Tsutsui, Satoshi; Sugimoto, Kunihisa; Tsunoda, Ryoma; Hirose, Yusuke; Mito, Takeshi; Settai, Rikio; Mizumaki, Masaichiro

    2016-06-01

    A diffraction experiment using high-energy X-rays was carried out on YbInCu4. Below the Yb valence transition temperature, the splitting of Bragg peaks was detected in high-order reflections. No superlattice reflections accompanying the valence ordering were found below the transition temperature. These experimental findings indicate that a structural change from a cubic structure to a tetragonal structure without valence ordering occurs at the transition temperature. Such a structural change free from any valence ordering is difficult to understand only in terms of Yb valence degrees of freedom. This means that the structural change may be related to electronic symmetries such as quadrupolar degrees of freedom as well as to the change in Yb valence.

  11. Modeling of chemical transition of nitrate accompanied with corrosion of carbon steel under alkaline conditions

    Interaction between carbon steel and nitrate was modeled using the mixed potential concept. Carbon steel was selected as an example of metal components in the repository of radioactive wastes. The nitrate reduction accompanied with the corrosion of carbon steel was modeled as a reaction series of NO3- → NO2- → NH3. The sum of the current of the reaction series of NO3- → NO2- → NH3 and that of water reduction was assumed to be balanced with the oxidation current of carbon steel. The input parameters for this kinetic model were determined by electrochemical measurements and immersion tests. The results of the immersion tests can be interpreted by the analyses of the model. (author)

  12. Nutcracker Syndrome Accompanying Pelvic Congestion Syndrome; Color Doppler Sonography and Multislice CT Findings: A Case Report

    Nutcracker syndrome (NCS) is a rare pathology, caused by compression of the left renal vein (LRV) between the abdominal aorta (AA) and the superior mesenteric artery (SMA), due to reduction of the angle between AA and SMA. This leads to LRV varices, left gonadal vein varices and therefore, the pelvic congestion syndrome. For this reason, coexistence of NCS and pelvic congestion syndrome has been described. It manifests by hematuria, proteinuria, and nonspecific pelvic pain secondary to pelvic congestion, dyspareunia and persistent genital arousal. We report a 27-year-old woman who experienced hematuria and left flank pain. The diagnosis of NCS accompanied by pelvic congestion syndrome was missed initially, but later on the diagnosis was made by color Doppler ultrasound, abdominal computed tomography (CT) and CT angiography that were later performed. She refused interventional and surgical treatments, and was lost to follow up

  13. Theory of the Jitter radiation in a magnetized plasma accompanying temperature gradient

    Hattori, Makoto

    2016-01-01

    The linear stability of a magnetized plasma accompanying temperature gradient was reexamined by using plasma kinetic theory. The anisotropic velocity distribution function was decomposed into two components. One is proportional to the temperature gradient parallel to and the other is proportional to the temperature gradient perpendicular to the back ground magnetic field. Since the amplitude of the anisotropic velocity distribution function is proportional to the heat conductivity and the heat conductivities perpendicular to the magnetic field is strongly reduced, the first component of the anisotropic velocity distribution function is predominant. The anisotropic velocity distribution function induced by the temperature gradient along the back ground magnetic field drives plasma kinetic instability and the circular polarized magnetic plasma waves are excited. The instability is almost identical to Weibel instability in weakly magnetized plasma. However, depending on whether wave vectors of modes are parallel...

  14. Successful repair of a syphilitic aortic arch aneurysm accompanied by serious cerebral infarction.

    Sato, Katsutoshi; Chiba, Kiyoshi; Koizumi, Nobusato; Ogino, Hitoshi

    2014-01-01

    We present a 52-year-old male with a syphilitic aortic arch aneurysm accompanied by relevant extensive cerebral infarction. He was admitted to a local hospital for sudden loss of consciousness, where he was diagnosed with serious cerebral infarction. During his treatment, a multilocular aortic arch aneurysm involving the arch vessels was found incidentally. He was transferred to our hospital for surgical treatment. A preoperative routine laboratory test for syphilis was highly positive, which suggested that the aneurysm was likely caused by syphilis and the cerebral infarction was also induced by the involvement of syphilitic aortitis or arteritis. After 2 weeks of antibiotic therapy for syphilis, total arch replacement was performed successfully using meticulous brain protection with antegrade selective cerebral perfusion and deep hypothermia. He recovered without any further cerebral deficits. The pathological examination of the surgical specimen showed some characteristic changes of syphilitic aortitis. PMID:24492165

  15. Graphene growth from reduced graphene oxide by chemical vapour deposition: seeded growth accompanied by restoration

    Chang, Sung-Jin; Hyun, Moon Seop; Myung, Sung; Kang, Min-A.; Yoo, Jung Ho; Lee, Kyoung G.; Choi, Bong Gill; Cho, Youngji; Lee, Gaehang; Park, Tae Jung

    2016-03-01

    Understanding the underlying mechanisms involved in graphene growth via chemical vapour deposition (CVD) is critical for precise control of the characteristics of graphene. Despite much effort, the actual processes behind graphene synthesis still remain to be elucidated in a large number of aspects. Herein, we report the evolution of graphene properties during in-plane growth of graphene from reduced graphene oxide (RGO) on copper (Cu) via methane CVD. While graphene is laterally grown from RGO flakes on Cu foils up to a few hundred nanometres during CVD process, it shows appreciable improvement in structural quality. The monotonous enhancement of the structural quality of the graphene with increasing length of the graphene growth from RGO suggests that seeded CVD growth of graphene from RGO on Cu surface is accompanied by the restoration of graphitic structure. The finding provides insight into graphene growth and defect reconstruction useful for the production of tailored carbon nanostructures with required properties.

  16. Graphene growth from reduced graphene oxide by chemical vapour deposition: seeded growth accompanied by restoration

    Chang, Sung-Jin; Hyun, Moon Seop; Myung, Sung; Kang, Min-A; Yoo, Jung Ho; Lee, Kyoung G.; Choi, Bong Gill; Cho, Youngji; Lee, Gaehang; Park, Tae Jung

    2016-01-01

    Understanding the underlying mechanisms involved in graphene growth via chemical vapour deposition (CVD) is critical for precise control of the characteristics of graphene. Despite much effort, the actual processes behind graphene synthesis still remain to be elucidated in a large number of aspects. Herein, we report the evolution of graphene properties during in-plane growth of graphene from reduced graphene oxide (RGO) on copper (Cu) via methane CVD. While graphene is laterally grown from RGO flakes on Cu foils up to a few hundred nanometres during CVD process, it shows appreciable improvement in structural quality. The monotonous enhancement of the structural quality of the graphene with increasing length of the graphene growth from RGO suggests that seeded CVD growth of graphene from RGO on Cu surface is accompanied by the restoration of graphitic structure. The finding provides insight into graphene growth and defect reconstruction useful for the production of tailored carbon nanostructures with required properties. PMID:26961409

  17. On description of photonuclear reactions accompanied by excitation of the giant dipole resonance

    Rodin, V A

    2002-01-01

    A semimicroscopical approach is applied to describe the photoabsorption and partial photonucleon reactions accompanied by excitation of the giant dipole resonance (GDR). The approach is based on both continuum-CRPA (CRPA) and a phenomenological description for doorway-state coupling to many-quasiparticle configurations. Apart from a phenomenological mean field, the separable isovector momentum-dependent forces and momentum-independent Landau-Migdal particle-hole interaction are used as the input quantities for the CRPA calculations. The photoabsorption and partial $(n,\\gamma)$-reaction cross sections in the vicinity of the GDR are satisfactorily described for $^{89}$Y, $^{140}$Ce and $^{208}$Pb target nuclei. An attempt to evaluate the total direct-neutron-decay branching ratio of the GDR in $^{48}$Ca and $^{208}$Pb is also undertaken.

  18. Tubal cytokine changes accompany the epithelial atypia of letrozole-stimulated ovaries.

    Abdel-Hamid, Ahmed A M; Mesbah, Yaser; Farouk, Mona F M

    2016-04-01

    Letrozole (LTZ), one of ovulation induction medications, is increasingly prescribed in many gynecological conditions. Although its hazardous effect on the ovarian surface epithelium (OSE) as well as tubal epithelium cells (TEC) has been previously studied, the associated changes occurring in the inflammatory cytokines have not been elucidated. Therefore, the objective of our study is to investigate these changes that may accompany LTZ-induced tubo-ovarian epithelial abnormalities. A total of 45 Sprague-Dawley rats were used in this study, divided equally into; control, LTZ6 and LTZ12 groups (received saline, 6 and 12 cycles LTZ i.p. respectively). Samples from ovaries (OVs) as well as fallopian tubes (FTs) were histologically studied for the associated changes. An increased proliferative activity, Ki67 immunoexpression and abnormal invaginations were observed in the OSE of LTZ6 group accompanied with occasional pseudostratification and loss of cilia of TEC. These changes became more pronounced in the LTZ12 where micropapillae, hyperchromasia, frequent deep invaginations, cysts of OSE as well as papillae and multilayering of TEC were noticed. The tubal level of IL-1β, IL-6, TNF-α and serum MCP-1 progressively increased in LTZ6 and LTZ12 groups compared with the control group. The significant positive correlation observed between these cytokines in the LTZ6 group became stronger in the LTZ12 one. However, no significant changes in the tubal IL-10 and TGF-β were detected. Therefore, further studies are required to consider these cytokines as objective markers to precisely assess severity of the associated epithelial changes particularly in long periods of stimulation. PMID:26837861

  19. Effects of Herbal Acupuncture(Soyeum on Cancer Patients Accompanied by Pain

    Hwa-Seung Yoo

    2003-02-01

    Full Text Available Objectives : This study was designed to evaluate effects of "Soyeum" on cancer patients accompanied by pain. Materials and Methods : We retrospectively analyzed the medical records of 9 patients accompanied by pain who had been injected with "Soyeum" for over 14 days continuously in East-West Cancer Center of Oriental Hospital of Daejeon University from June 2002 through August 2002. Results : The statistical significance between the pre-treatment and post-treatment results (Changes of Cytokine Level, QOL, BPQ and Pain relief after pain management were analyzed. Analysis of cytokines (IL-12, IFN-γ level showed that the percentage of increase of IL-12 is 60.0%, IFN-γ is 60.0%. Analysis of QOL showed that the percentage of maintenance and improvement is 77.8%. 55.5% of the patients reported a "worst pain" intensity score of 3 or greater, 44.5% reported a "least pain" intensity score of 2 or greater, and 66.7% reported "average pain" intensity score of 2 or greater. 33.3% of the patients were in pain at the time of interview and 22.2% had a current intensity score of 2 or greater. Analysis of pain relief after pain management showed that the percentage of pain relief score of 2 and 3 is 55.6%. The data was expressed as Mean±SE by using descriptive statistics. Statistical significance examined by using paired t-test. Conclusions : It is suggested that "Soyeum" has effects on pain of cancer patients, also expected that "Soyeum" is useful to improve immunoactivity and for cancer patients.

  20. Collagen coated tantalum substrate for cell proliferation.

    Li, Yinli; Zhang, Shuai; Guo, Lijun; Dong, Mingdong; Liu, Bo; Mamdouh, Wael

    2012-06-15

    The extracellular matrix (ECM) plays a key role in cell culture in various physiological and pathological processes in the field of tissue engineering. Recently, the type I collagen ECM has been widely utilized in vitro model systems for the attachment of many different cell lines since it has multi-functions in human tissues. For example it accounts for 6% of the weight of strong, tendinous muscles. In this paper, we reported a new material by coating tantalum (Ta), one highly biocompatible metal, with type I collagen fibrils. The morphology of the new material was studied by high resolution atomic force microscope. It was shown that the adhesion force between type I collagen fibrils network and Ta was strong enough to overcome surface defects. A possible way to explain the phenomenon is that the longitudinal periodicity of collagen fibrils matches the grain size of the Ta domains, which results in increase of the physical adsorption contact area, thereby inducing the dramatic adhesion enhancement between collagen fibrils and Ta. The obtained material was then employed as a template for cell proliferation. Although the surface of this template is more hydrophobic by comparison with the bare Ta surface, the cells on this material were successfully incubated, indicating that the collagen coated Ta might be used as the buffer layer for proliferating cells in hydrophobic biomaterials. PMID:22494669

  1. Non-proliferation policy and safeguards

    At the beginning of '90s the international community and IAEA have been confronted with serious problems related to the maintaining of the world non-proliferation policy. At the end of the cold war, rumors regarding Iraq's nuclear armament secret program and a new democracies' perception regarding nuclear safety have given a strong impetus toward reinforcing and redefining the safeguards systems. Today this system has got the most advanced and modern means for the detection of nuclear materials stealing and surreptitious production. At the same time with the implementation of the new additional model of safeguards agreements, every country developed an important contribution to end this period in which proliferation and threatening of nuclear weapons were a ruling feature on the international scene. The paper presents the principles of IAEA and national nuclear safeguards system. Mentioned are the techniques and equipment for nuclear safeguards control applied particularly at Cernavoda NPP and TRIGA reactor at IRN - Pitesti as well as those related to the evaluation of the plutonium content in the irradiated fuel. In Romania largely utilized was the program ORIGEN

  2. Non-proliferation of nuclear weapons

    During the turbulant transitional events in world politics in the nineties, the control of nuclear weapons plays a major role. While the superpowers are reducing their nuclear arsenal, the danger of nuclear anarchy in the world remains virulent. The NPT of 1968 is up for review soon. The falling apart of the former communist sphere of power, and the regions of conflict in the Third World present new risks for the proliferation of nuclear arms. For unified Germany, which explicitly renounced nuclear weapons, this situation presents difficult questions concerning national safety policies and international responsibility. This volume presents contributions which take a new look at topical and long-term problems of nuclear NP politics. The authors evaluate the conditions under which the NP regime came into being, and assess short- and long-term possibilities and risks. The following papers are included: 1.) Basic controversies during the negotiations concerning the Treaty on non-proliferation of nuclear weapons (Ungerer); 2.) Prologation of the NPT 1995 and appropriate problems concerning safety and control (Haefele/Lauppe); 3.) Consequences of the Iraq case for NP policy (Ficher); 4.) Problems of nuclear technology control (Mueller); 5.) Framework conditions of a nuclear world system (Haeckel). (orig./HP)

  3. Missile non-proliferation: an alternative approach

    In this report, the author first proposes an overview of the notion of missile prohibition. He notices that the association between weapons of mass destruction and missiles is a prelude to the legitimacy of missile control, notably within the framework of the Missile Technology Control Regime or MTCR. He also comments the notion of total ban. In a second part, the author analyses and discusses the limitations of the control of technology diffusion. He discusses the role of the MTCR, comments the evolution of this regime with the taking of China and Russia into consideration, the impacts of national implementations of export regimes on the MTCR, and economic aspects of control implementation. In the next part, the author addresses other kinds of limitations, i.e. those related with capacity evolutions of proliferating States. The last part addresses the evolution towards a new definition of approach to missile non-proliferation, notably in terms of perception of missile roles and of technology transfer controls

  4. Proliferation and fission of peroxisomes - An update.

    Schrader, Michael; Costello, Joseph L; Godinho, Luis F; Azadi, Afsoon S; Islinger, Markus

    2016-05-01

    In mammals, peroxisomes perform crucial functions in cellular metabolism, signalling and viral defense which are essential to the health and viability of the organism. In order to achieve this functional versatility peroxisomes dynamically respond to molecular cues triggered by changes in the cellular environment. Such changes elicit a corresponding response in peroxisomes, which manifests itself as a change in peroxisome number, altered enzyme levels and adaptations to the peroxisomal structure. In mammals the generation of new peroxisomes is a complex process which has clear analogies to mitochondria, with both sharing the same division machinery and undergoing a similar division process. How the regulation of this division process is integrated into the cell's response to different stimuli, the signalling pathways and factors involved, remains somewhat unclear. Here, we discuss the mechanism of peroxisomal fission, the contributions of the various division factors and examine the potential impact of post-translational modifications, such as phosphorylation, on the proliferation process. We also summarize the signalling process and highlight the most recent data linking signalling pathways with peroxisome proliferation. PMID:26409486

  5. Some mathematics of nuclear weapons proliferation

    Full text: In the many decades since the first demonstration of the power of nuclear weapons we have created a 'data set' that we may analyze to see if simple principles account for the observed history. There was initial fear that the proliferation of weapons would be exponential, driven by the principle that each nation would desire to have weapons to deter each other nuclear-armed nation. The doubling time could be taken as four years, between the tests of the US in 1945 and the USSR in 1949. By 1985, with ten doubling times, we would have had 1024 nuclear weapons states, far from the data. If we make a different hypothesis, that each nation is deterred from seeking nuclear weapons by each existing nuclear state, we obtain a differential equation leading to an expression for a much slower rate of increase, which is indeed found to be closely followed. If we have an understanding of the processes that lead to the proliferation of nuclear weapons, we can be better at strengthening the barriers to further growth. The mathematical equations and relevant plots will be used to illustrate these observations. (author)

  6. Nuclear proliferation: Some context and consequences

    The article addressed the importance of nonproliferation and supporting and reinforcing nonproliferation commitments. The most important benefit of the NPT has been in its contribution to the security of individual states party, as well as to regional and international security, through the obligations which help to prevent any further proliferation of nuclear weapons. The NPT therefore powerfully augments the national security of every state party, and not merely just the NPT nuclear weapons states. Indeed, it is the countries of the developing world, as well as many other non-nuclear weapon states, that could suffer the most in security terms if a non-nuclear weapons state in the developing world suddenly acquired The Bomband became emboldened to engage in threats and adventurism against its neighbors. The nonproliferation regime hinges upon the steps that all countries take on their own and with like-minded allies to further nonproliferation goals - and whether the international community can successfully shape the calculations of present-day and future would-be proliferation in useful ways. The nonproliferation regime, therefore, includes not just the NPT and other legally-binding obligations but complex dynamics of persuasion and deterrence that employ many different tools. The NPT helps establish the core nonproliferation obligations toward which many of the tools in the international community's tool kit are directed.

  7. Extracts of proliferating and non-proliferating human cells display different base excision pathways and repair fidelity

    Akbari, Mansour; Pena Diaz, Javier; Andersen, Sonja;

    2009-01-01

    cells both had capacity for single- and two-nucleotide insertion BER activity. However, patch size longer than two nucleotides was only detected in extracts from proliferating cells. Relative to extracts from proliferating cells, extracts from non-proliferating cells had approximately two-fold higher...... concentration of POLbeta, which contributed to most of two-nucleotide insertion BER. In contrast, two-nucleotide insertion in extracts from proliferating cells was not dependent on POLbeta. BER fidelity was two- to three-fold lower in extracts from the non-proliferating compared with extracts of proliferating...... cells. Furthermore, although one-nucleotide deletion was the predominant type of repair error in both extracts, the pattern of repair errors was somewhat different. These results establish two-nucleotide patch BER as a distinct POLbeta-dependent mechanism in non-proliferating cells and demonstrate that...

  8. Reducing the Risk of Damage to Power Transformers of 110 kV and Above Accompanying Internal Short Circuits

    Methods of increasing the operating reliability of power transformers, autotransformers and shunting reactors in order to reduce the risk of damage, which accompany internal short circuits and equipment fires and explosions, are considered

  9. Is Our CD/DVD Collection Worth All This? A Cost-Per-Use Study of Accompanying Materials

    Kennedy, Marie R.

    2007-01-01

    This research reports on a study designed to quantify the time and monies spent on cataloging and processing texts with accompanying materials. The study was designed to address a need for data to assist in collection development and workflow decisions.

  10. Proliferation and the Civilian Nuclear Fuel Cycle. Towards a Simplified Recipe to Measure Proliferation Risk

    The primary goal of this study is to frame the problem of nuclear proliferation in the context of protection and risks associated with nuclear materials flowing in the civilian nuclear fuel cycle. The perspective adopted for this study is that of a nuclear utility and the flow of fresh and spent nuclear fuel with which that utility must deal in the course of providing economic, safe, and ecologically acceptable electrical power to the public. Within this framework quantitative approaches to a material-dependent, simplified proliferation-risk metric are identified and explored. The driving force behind this search for such a proliferation metric derives from the need to quantify the proliferation risk in the context of evaluating various commercial nuclear fuel cycle options (e.g., plutonium recycle versus once-through). While the formulation of the algebra needed to describe the desired, simplified metric(s) should be straight forward once a modus operandi is defined, considerable interaction with the user of any final product that results is essential. Additionally, a broad contextual review of the proliferation problem and past efforts in the quantification of associated risks was developed as part of this study. This extensive review was essential to setting perspectives and establishing (feasibility) limits to the search for a proliferation metric(s) that meets the goals of this study. Past analyses of proliferation risks associated with the commercial nuclear fuel cycle have generally been based on a range of decision-analysis, operations-research tools. Within the time and budget constraints, as well as the self-enforced (utility) customer focus, the more subjective and data-intensive decision-analysis methodologies where not pursued. Three simplified, less-subjective approaches were investigated instead: a) a simplified 'four-factor' formula expressing as a normalized product political, material-quantity, material-quality, and material-protection metrics; b

  11. Proliferation and the Civilian Nuclear Fuel Cycle. Towards a Simplified Recipe to Measure Proliferation Risk

    Brogli, R.; Krakowski, R.A

    2001-08-01

    The primary goal of this study is to frame the problem of nuclear proliferation in the context of protection and risks associated with nuclear materials flowing in the civilian nuclear fuel cycle. The perspective adopted for this study is that of a nuclear utility and the flow of fresh and spent nuclear fuel with which that utility must deal in the course of providing economic, safe, and ecologically acceptable electrical power to the public. Within this framework quantitative approaches to a material-dependent, simplified proliferation-risk metric are identified and explored. The driving force behind this search for such a proliferation metric derives from the need to quantify the proliferation risk in the context of evaluating various commercial nuclear fuel cycle options (e.g., plutonium recycle versus once-through). While the formulation of the algebra needed to describe the desired, simplified metric(s) should be straight forward once a modus operandi is defined, considerable interaction with the user of any final product that results is essential. Additionally, a broad contextual review of the proliferation problem and past efforts in the quantification of associated risks was developed as part of this study. This extensive review was essential to setting perspectives and establishing (feasibility) limits to the search for a proliferation metric(s) that meets the goals of this study. Past analyses of proliferation risks associated with the commercial nuclear fuel cycle have generally been based on a range of decision-analysis, operations-research tools. Within the time and budget constraints, as well as the self-enforced (utility) customer focus, the more subjective and data-intensive decision-analysis methodologies where not pursued. Three simplified, less-subjective approaches were investigated instead: a) a simplified 'four-factor' formula expressing as a normalized product political, material-quantity, material-quality, and material

  12. PPARα: Mechanism of species differences and hepatocarcinogenesis of peroxisome proliferators

    Peroxisome proliferator chemicals are classic non-genotoxic carcinogens. These agents cause liver cancers when chronically administered to rats and mice. Peroxisome proliferators include the widely prescribed lipid and cholesterol lowering fibrate drugs. In contrast to the results in rodents, there is no evidence that fibrates are associated with elevated risk of liver cancer or any other neoplasms in humans thus indicating a species difference in the hepatocarcinogenic response. The biological effects of peroxisome proliferators are mediated by the peroxisome proliferator-activated receptor (PPAR)α. Pparα-null mice are resistant to all of the pleiotropic effects of peroxisome proliferators, including cell proliferation and hepatocarcinogenesis. The mechanism of hepatocellular proliferation involves downregulation of the microRNA let-7c gene by PPARα. Let-7c controls levels of proliferative c-myc by destabilizing its mRNA. Thus, upon suppression of let-7c, c-myc mRNA and protein are elevated resulting in enhanced hepatocellular proliferation. In contrast, PPARα-humanized mice, that respond to Wy-14,643 by lower serum triglycerides and induction of genes encoding fatty acid metabolizing enzymes, are resistant to peroxisome proliferator-induced cell proliferation and cancer. These mice do not exhibit downregulation of let-7c gene expression thus forming the basis for the resistance to hepatocellular carcinogenesis

  13. {\\alpha}-accompanied cold ternary fission of $^{238-244}$Pu isotopes in equatorial and collinear configuration

    Santhosh, K P; Krishnan, Sreejith; Priyanka, B.

    2015-01-01

    The cold ternary fission of $^{238}$Pu, $^{240}$Pu, $^{242}$Pu and $^{244}$Pu isotopes, with $^{4}$He as light charged particle, in equatorial and collinear configuration has been studied within the Unified ternary fission model (UTFM). The fragment combination $^{100}$Zr+$^{4}$He+$^{134}$Te possessing the near doubly magic nuclei $^{134}$Te (N=82, Z=52) gives the highest yield in the alpha accompanied ternary fission of $^{238}$Pu. For the alpha accompanied ternary fission of $^{240}$Pu, $^{...

  14. Peptic ulcers accompanied with gastrointestinal bleeding, pylorus obstruction and cholangitis secondary to choledochoduodenal fistula: A case report

    Xi, Bin; JIA, JUN-JUN; Lin, Bing-Yi; Geng, Lei; Zheng, Shu-Sen

    2015-01-01

    Peptic ulcers are an extremely common condition, usually occurring in the stomach and proximal duodenum. However, cases of peptic ulcers accompanied with multiple complications are extremely rare and hard to treat. The present case reinforces the requirement for the early recognition and correct treatment of peptic ulcers accompanied with multiple complications. A 67-year-old man presented with recurrent abdominal pain, fever and melena. The laboratory results showed anemia (hemoglobin 62 g/l...

  15. Can light water reactors be proliferation resistant?

    During the last decade several questions were raised concerning the proliferation issues of Light Water Reactors (LWRs) in comparison with other types of power reactors, particularly Gas Cooled Reactors (GCRs) and Heavy Water Reactors (HWRs). These questions were strongly highlighted when the Agreed Framework between the United States and the DPRK was signed in October 1994 and following the formation of KEDO organization to provide two LWRs to DPRK in replacement of all its GCRs in its nuclear program. One might summarize the main questions into three groups, mainly: 1. Can LWRs produce weapon-grade Plutonium (Pu)? 2. Why is the LWR type considered as a better option with regard to non-proliferation compared to other power reactors - particularly GCR and HWR types? 3. How could LWRs be more resistant to proliferation? This paper summarizes the effort to answer these questions. Included tables present numerical parameters for Pu production capability of the three main reactor types (LWRs, GCRs and HWRs) of a 400 MWe power reactor unit, during normal operation, and during abnormal operation to produce weapon grade Pu. Can LWRs produce weapon-grade Pu? It is seen from the available data that weapon-grade Pu could be produced in LWR fuel, as in the fuel of most other power reactor types, by limiting fuel irradiation to two or three months only. However, such production, though possible, is exceptional. In a recent study 5% of LWRs under IAEA safeguards have spent fuel inventory containing limited amount of high-grade Pu. The equilibrium burnup of discharged fuel is in the order of 33,000 MWD/T. However and due to lower enrichment of initial inventory almost half of that burnup is produced. In normal situations the discharged initial inventory has a Pu grade which is less than weapon grade and is unlikely to be used for weapon production. Why LWR the type is considered as a better option for non-proliferation Referring to tables, one can conclude that LWRs make less Pu

  16. Increased activity of chondroitin sulfate-synthesizing enzymes during proliferation of arterial smooth muscle cells

    Cultured arterial smooth muscle cells incorporate [35S]sulfate into the extracellular chondroitin sulfate/dermatan sulfate containing proteoglycans at a higher rate in the phase of logarithmic growth than do non-dividing cells. The cell growth-dependent decrease in 35S incorporation with increasing cell density is accompanied by a decrease in the activity of chondroitin sulfate-synthesizing enzymes. The specific activity of xylosyl transferase, N-acetylgalactosaminyl transferase I and chondroitin sulfotransferase declines as the cells proceed from low to high densities. The corresponding correlation coefficients are 0.86, 0.91 and 0.89. The ratio of C-60H/C-40H sulfation of chondroitin shows a cell proliferation-dependent decrease indicating an inverse correlation of chondroitin 6-sulfotransferase and chondroitin 4-sulfotransferase activity. The observed changes in the expression of enzyme activities are thought to have some implications in the pathogenesis of arteriosclerosis, the initial stages of which are characterized by proliferation of arterial smooth muscle cells

  17. Increased activity of chondroitin sulfate-synthesizing enzymes during proliferation of arterial smooth muscle cells

    Hollmann, J.; Thiel, J.; Schmidt, A.; Buddecke, E.

    1986-12-01

    Cultured arterial smooth muscle cells incorporate (/sup 35/S)sulfate into the extracellular chondroitin sulfate/dermatan sulfate containing proteoglycans at a higher rate in the phase of logarithmic growth than do non-dividing cells. The cell growth-dependent decrease in /sup 35/S incorporation with increasing cell density is accompanied by a decrease in the activity of chondroitin sulfate-synthesizing enzymes. The specific activity of xylosyl transferase, N-acetylgalactosaminyl transferase I and chondroitin sulfotransferase declines as the cells proceed from low to high densities. The corresponding correlation coefficients are 0.86, 0.91 and 0.89. The ratio of C-60H/C-40H sulfation of chondroitin shows a cell proliferation-dependent decrease indicating an inverse correlation of chondroitin 6-sulfotransferase and chondroitin 4-sulfotransferase activity. The observed changes in the expression of enzyme activities are thought to have some implications in the pathogenesis of arteriosclerosis, the initial stages of which are characterized by proliferation of arterial smooth muscle cells.

  18. Recombinant adenovirus of human p66Shc inhibits MCF-7 cell proliferation.

    Yang, Xiaoshan; Xu, Rong; Lin, Yajun; Zhen, Yongzhan; Wei, Jie; Hu, Gang; Sun, Hongfan

    2016-01-01

    The aim of this work was to construct a human recombinant p66Shc adenovirus and to investigate the inhibition of recombinant p66Shc adenovirus on MCF-7 cells. The recombinant adenovirus expression vector was constructed using the Adeno-X Adenoviral System 3. Inhibition of MCF-7 cell proliferation was determined by MTT. Intracellular ROS was measured by DCFH-DA fluorescent probes, and 8-OHdG was detected by ELISA. Cell apoptosis and the cell cycle were assayed by flow cytometry. Western blot were used to observe protein expression. p66Shc expression was upregulated in 4 cell lines after infection. The inhibitory effect of p66Shc recombinant adenovirus on MCF-7 cells was accompanied by enhanced ROS and 8-OHdG. However, no significant differences were observed in the cell apoptosis rate. The ratio of the cell cycle G2/M phase showed a significant increase. Follow-up experiments demonstrated that the expressions of p53, p-p53, cyclin B1 and CDK1 were upregulated with the overexpression of p66Shc. The Adeno-X Adenoviral System 3 can be used to efficiently construct recombinant adenovirus containing p66Shc gene, and the Adeno-X can inhibit the proliferation of MCF-7 cells by inducing cell cycle arrest at the G2/M phase. These results suggested that p66Shc may be a key target for clinical cancer therapy. PMID:27530145

  19. Antibiotic drug tigecycline inhibited cell proliferation and induced autophagy in gastric cancer cells

    Highlights: • Tigecycline inhibited cell growth and proliferation in human gastric cancer cells. • Tigecycline induced autophagy not apoptosis in human gastric cancer cells. • AMPK/mTOR/p70S6K pathway was activated after tigecycline treatment. • Tigecycline inhibited tumor growth in xenograft model of human gastric cancer cells. - Abstract: Tigecycline acts as a glycylcycline class bacteriostatic agent, and actively resists a series of bacteria, specifically drug fast bacteria. However, accumulating evidence showed that tetracycline and their derivatives such as doxycycline and minocycline have anti-cancer properties, which are out of their broader antimicrobial activity. We found that tigecycline dramatically inhibited gastric cancer cell proliferation and provided an evidence that tigecycline induced autophagy but not apoptosis in human gastric cancer cells. Further experiments demonstrated that AMPK pathway was activated accompanied with the suppression of its downstream targets including mTOR and p70S6K, and ultimately induced cell autophagy and inhibited cell growth. So our data suggested that tigecycline might act as a candidate agent for pre-clinical evaluation in treatment of patients suffering from gastric cancer

  20. Vimentin expression influences flow dependent VASP phosphorylation and regulates cell migration and proliferation

    The cytoskeleton plays a central role for the integration of biochemical and biomechanical signals across the cell required for complex cellular functions. Recent studies indicate that the intermediate filament vimentin is necessary for endothelial cell morphogenesis e.g. in the context of leukocyte transmigration. Here, we present evidence, that the scaffold provided by vimentin is essential for VASP localization and PKG mediated VASP phosphorylation and thus controls endothelial cell migration and proliferation. Vimentin suppression using siRNA technique significantly decreased migration velocity by 50% (videomicroscopy), diminished transmigration activity by 42.5% (Boyden chamber) and reduced proliferation by 43% (BrdU-incorporation). In confocal microscopy Vimentin colocalized with VASP and PKG in endothelial cells. Vimentin suppression was accompanied with a translocation of VASP from focal contacts to the perinuclear region. VASP/Vimentin and PKG/Vimentin colocalization appeared to be essential for proper PKG mediated VASP phosphorylation because we detected a diminished expression of PKG and pSer239-VASP in vimentin-suppressed cells, Furthermore, the induction of VASP phosphorylation in perfused arteries was markedly decreased in vimentin knockout mice compared to wildtypes. A link is proposed between vimentin, VASP phosphorylation and actin dynamics that delivers an explanation for the important role of vimentin in controlling endothelial cell morphogenesis.

  1. Running Exercise Alleviates Pain and Promotes Cell Proliferation in a Rat Model of Intervertebral Disc Degeneration

    Shuo Luan

    2015-01-01

    Full Text Available Chronic low back pain accompanied by intervertebral disk degeneration is a common musculoskeletal disorder. Physical exercise, which is clinically recommended by international guidelines, has proven to be effective for degenerative disc disease (DDD patients. However, the mechanism underlying the analgesic effects of physical exercise on DDD remains largely unclear. The results of the present study showed that mechanical withdrawal thresholds of bilateral hindpaw were significantly decreased beginning on day three after intradiscal complete Freund’s adjuvant (CFA injection and daily running exercise remarkably reduced allodynia in the CFA exercise group beginning at day 28 compared to the spontaneous recovery group (controls. The hindpaw withdrawal thresholds of the exercise group returned nearly to baseline at the end of experiment, but severe pain persisted in the control group. Histological examinations performed on day 70 revealed that running exercise restored the degenerative discs and increased the cell densities of the annulus fibrosus (AF and nucleus pulposus (NP. Furthermore, immunofluorescence labeling revealed significantly higher numbers of 5-bromo-2-deoxyuridine (BrdU-positive cells in the exercise group on days 28, 42, 56 and 70, which indicated more rapid proliferation compared to the control at the corresponding time points. Taken together, these results suggest that running exercise might alleviate the mechanical allodynia induced by intradiscal CFA injection via disc repair and cell proliferation, which provides new evidence for future clinical use.

  2. Antibiotic drug tigecycline inhibited cell proliferation and induced autophagy in gastric cancer cells

    Tang, Chunling; Yang, Liqun; Jiang, Xiaolan [State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716 (China); Xu, Chuan [Division of Scientific Research and Training, General Hospital of PLA Chengdu Military Area Command, Chengdu, Sichuan 610083 (China); Wang, Mei; Wang, Qinrui [State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716 (China); Zhou, Zhansong, E-mail: zhouzhans@sina.com [Institute of Urinary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038 (China); Xiang, Zhonghuai [State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716 (China); Cui, Hongjuan, E-mail: hcui@swu.edu.cn [State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716 (China)

    2014-03-28

    Highlights: • Tigecycline inhibited cell growth and proliferation in human gastric cancer cells. • Tigecycline induced autophagy not apoptosis in human gastric cancer cells. • AMPK/mTOR/p70S6K pathway was activated after tigecycline treatment. • Tigecycline inhibited tumor growth in xenograft model of human gastric cancer cells. - Abstract: Tigecycline acts as a glycylcycline class bacteriostatic agent, and actively resists a series of bacteria, specifically drug fast bacteria. However, accumulating evidence showed that tetracycline and their derivatives such as doxycycline and minocycline have anti-cancer properties, which are out of their broader antimicrobial activity. We found that tigecycline dramatically inhibited gastric cancer cell proliferation and provided an evidence that tigecycline induced autophagy but not apoptosis in human gastric cancer cells. Further experiments demonstrated that AMPK pathway was activated accompanied with the suppression of its downstream targets including mTOR and p70S6K, and ultimately induced cell autophagy and inhibited cell growth. So our data suggested that tigecycline might act as a candidate agent for pre-clinical evaluation in treatment of patients suffering from gastric cancer.

  3. Numb-deficient satellite cells have regeneration and proliferation defects.

    George, Rajani M; Biressi, Stefano; Beres, Brian J; Rogers, Erik; Mulia, Amanda K; Allen, Ronald E; Rawls, Alan; Rando, Thomas A; Wilson-Rawls, Jeanne

    2013-11-12

    The adaptor protein Numb has been implicated in the switch between cell proliferation and differentiation made by satellite cells during muscle repair. Using two genetic approaches to ablate Numb, we determined that, in its absence, muscle regeneration in response to injury was impaired. Single myofiber cultures demonstrated a lack of satellite cell proliferation in the absence of Numb, and the proliferation defect was confirmed in satellite cell cultures. Quantitative RT-PCR from Numb-deficient satellite cells demonstrated highly up-regulated expression of p21 and Myostatin, both inhibitors of myoblast proliferation. Transfection with Myostatin-specific siRNA rescued the proliferation defect of Numb-deficient satellite cells. Furthermore, overexpression of Numb in satellite cells inhibited Myostatin expression. These data indicate a unique function for Numb during the initial activation and proliferation of satellite cells in response to muscle injury. PMID:24170859

  4. Proliferating cell nuclear antigen (PCNA) activity in hepatocellular carcinoma, benign peri-neoplastic and normal liver.

    Mun, Kein-Seong; Cheah, Phaik-Leng; Baharudin, Nurul Bahiyah; Looi, Lai-Meng

    2006-12-01

    Hepatocellular carcinoma (HCC) is among the ten most common cancers in Malaysian males. As cellular proliferation is an important feature of malignant transformation, we studied the proliferation pattern of normal and benign perineoplastic liver versus hepatocellular carcinoma in an attempt to further understand the tumour transformation process. 39 HCC (21 with accompanying and 18 without cirrhosis) histologically diagnosed at the Department of Pathology, University of Malaya Medical Centre between January 1992 and December 2003 were immunohistochemically studied using a monoclonal antibody to PCNA (Clone PC10: Dako). 20 livers from cases who had succumbed to traumatic injuries served as normal liver controls (NL). PCNA labeling index (PCNA-LI) was determined by counting the number of immunopositive cells in 1000 contiguous HCC, benign cirrhotic perineoplastic liver (BLC), benign perineoplastic non-cirrhotic (BLNC) and NL cells and conversion to a percentage. The PCNA-LI was also expressed as Ojanguren et al's grades. PCNA was expressed in 10% NL, 38.9% BLNC, 76.2% BLC and 71.8% HCC with BLNC, BLC and HCC showing significantly increased (p cells being immunopositive) immunoreactivity on Ojanguren et al's grading system and only HCC demonstrated immunoreactivity which ranged up to grade 3 (75% of cells). From this study, there appears to be a generally increasing trend of proliferative activity from NL to BLNC to BLC and HCC. Nonetheless, BLNC and BLC, like NL, retained low PCNA-LI and only HCC had a significantly increased PCNA-LI compared with the benign categories. This is probably related to the malignant nature of HCC and may reflect the uncontrolled proliferation of the neoplastic hepatocytes. PMID:18376794

  5. Aquaporin 2-increased renal cell proliferation is associated with cell volume regulation.

    Di Giusto, Gisela; Flamenco, Pilar; Rivarola, Valeria; Fernández, Juan; Melamud, Luciana; Ford, Paula; Capurro, Claudia

    2012-12-01

    We have previously demonstrated that in renal cortical collecting duct cells (RCCD(1)) the expression of the water channel Aquaporin 2 (AQP2) raises the rate of cell proliferation. In this study, we investigated the mechanisms involved in this process, focusing on the putative link between AQP2 expression, cell volume changes, and regulatory volume decrease activity (RVD). Two renal cell lines were used: WT-RCCD(1) (not expressing aquaporins) and AQP2-RCCD(1) (transfected with AQP2). Our results showed that when most RCCD(1) cells are in the G(1)-phase (unsynchronized), the blockage of barium-sensitive K(+) channels implicated in rapid RVD inhibits cell proliferation only in AQP2-RCCD(1) cells. Though cells in the S-phase (synchronized) had a remarkable increase in size, this enhancement was higher and was accompanied by a significant down-regulation in the rapid RVD response only in AQP2-RCCD(1) cells. This decrease in the RVD activity did not correlate with changes in AQP2 function or expression, demonstrating that AQP2-besides increasing water permeability-would play some other role. These observations together with evidence implying a cell-sizing mechanism that shortens the cell cycle of large cells, let us to propose that during nutrient uptake, in early G(1), volume tends to increase but it may be efficiently regulated by an AQP2-dependent mechanism, inducing the rapid activation of RVD channels. This mechanism would be down-regulated when volume needs to be increased in order to proceed into the S-phase. Therefore, during cell cycle, a coordinated modulation of the RVD activity may contribute to accelerate proliferation of cells expressing AQP2. PMID:22786728

  6. Laser isotope separation and proliferation risks

    Fuss, Werner

    2015-02-15

    There is an ongoing discussion on the proliferation danger of laser enrichment of uranium by the Silex process. Here this risk is compared to that of other processes, in particular centrifuges. The two methods need a similar size of the plant for a comparable production rate (in separative work units per year) and the time and costs for their construction do not differ much. This conclusion from published material does not depend on technical details of Silex. But enough details are known to allow for additional conclusions: Whereas the selectivity (enrichment factor) in the Silex process seems higher, the energy consumption is probably larger. Due to the laser's repetition rate being insufficient for the molecular beam, the method has probably a low depletion factor; this is a serious disadvantage for cascading for high enrichment such as for bomb uranium, although it may be acceptable for low enrichment without cascading for reactor purposes.

  7. Intelligent hand-portable proliferation sensing system

    Argonne National Laboratory, with support from DOE's Office of Nonproliferation and National Security, is currently developing an intelligent hand-portable sensor system. This system is designed specifically to support the intelligence community with the task of in-field sensing of nuclear proliferation and related activities. Based upon pulsed laser photo-ionization time-of-flight mass spectrometry technology, this novel sensing system is capable of quickly providing a molecular or atomic analysis of specimens. The system is capable of analyzing virtually any gas phase molecule, or molecule that can be induced into the gas phase by (for example) sample heating. This system has the unique advantages of providing unprecedented portability, excellent sensitivity, tremendous fieldability, and a high performance/cost ratio. The system will be capable of operating in a highly automated manner for on-site inspections, and easily modified for other applications such as perimeter monitoring aboard a plane or drone. The paper describes the sensing system

  8. Cell proliferation inhibition in reduced gravity

    Moos, P. J.; Fattaey, H. K.; Johnson, T. C.; Spooner, B. S. (Principal Investigator)

    1994-01-01

    Extended durations of spaceflight have been shown to be deleterious on an organismic level; however, mechanisms underlying cellular sensitivity to the gravitational environment remain to be elucidated. The majority of the gravitational studies to date indicates that cell regulatory pathways may be influenced by their gravitational environment. Still, few cell biology experiments have been performed in space flight and even fewer experiments have been repeated on subsequent flights. With flight opportunities on STS-50, 54, and 57, Sf9 cells were flown in the BioServe Fluids Processing Apparatus and cell proliferation was measured with and without exposure to a cell regulatory sialoglycopeptide (CeReS) inhibitor. Results from these flights indicate that the Sf9 cells grew comparable to ground controls, that the CeReS inhibitor bound to its specific receptor, and that its signal transduction cascade was not gravity sensitive.

  9. Ballistic missile proliferation: An emerging threat 1992

    Nagler, R.G.

    1992-10-01

    This report, based solely on information available from unclassified sources, provides a coherent picture of the scope and trends of ballistic missile proliferation. The focus is on countries developing, producing, or owning ballistic missiles capable of threatening the military forces, assets, or populations of neighboring or geographically remote countries. The report also identifies other countries expected to obtain operational ballistic missile capabilities, discusses expected growth in performance, and examines the projected availability of warheads of mass destruction. The emphasis is on ballistic missiles of ranges greater than approximately 300 km, though shorter range battlefield weapons are discussed as forerunners. The assessment excludes principal U.S. allies and countries formerly in the Warsaw Pact, except where these countries have sold missiles, technology; or personnel services to developing nations in support of their missile programs.

  10. Skin cell proliferation stimulated by microneedles.

    Liebl, Horst; Kloth, Luther C

    2012-03-01

    A classical wound may be defined as a disruption of tissue integrity. Wounds, caused by trauma from accidents or surgery, that close via secondary intention rely on the biological phases of healing, i.e., hemostasis, inflammation, proliferation, and remodeling (HIPR). Depending on the wound type and severity, the inflammation phase begins immediately after injury and may last for an average of 7-14 days. Concurrent with the inflammation phase or slightly delayed, cell proliferation is stimulated followed by the activation of the remodeling (maturation) phase. The latter phase can last as long as 1 year or more, and the final healed state is represented by a scar tissue, a cross-linked collagen formation that usually aligns collagen fibers in a single direction. One may assume that skin microneedling that involves the use of dozens or as many as 200 needles that limit penetration to 1.5 mm over 1 cm(2) of skin would cause trauma and bleeding followed by the classical HIPR. However, this is not the case or at least the HIPR phases are significantly curtailed and healing never ends in a scar formation. Conversely dermabrasion used in aesthetic medicine for improving skin quality is based on "ablation" (destruction or wounding of superficial skin layers), which requires several weeks for healing that involves formation of new skin layers. Such procedures provoke an acute inflammatory response. We believe that a less intense inflammatory response occurs following microneedle perforation of the skin. However, the mechanism of action of microneedling appears to be different. Here we review the potential mechanisms by which microneedling of the skin facilitates skin repair without scarring after the treatment of superficial burns, acne, hyperpigmentation, and the non-advancing periwound skin surrounding the chronic ulcerations of the integument. PMID:24527373

  11. Evaluation of thermal water in patients with functional dyspepsia and irritable bowel syndrome accompanying constipation

    Giovanni Gasbarrini; Marcello Candelli; RiccardoGiuseppe Graziosetto; Sergio Coccheri; Ferdinando Di Iorio; Giuseppe Nappi

    2006-01-01

    AIM: To evaluate the efficacy of water supplementation treatment in patients with functional dyspepsia or irritable bowe syndrome (IBS) accompanying predominant constipation.METHODS: A total of 3 872 patients with functional dyspepsia and 3609 patients with irritable bowel syndrome were enrolled in the study by 18 Italina thermal centres.Patients underwent a first cycle of thermal therapy for 21 d. A year later patients were re-evaluated at the same centre and received another cycle of thermal therapy.A questionnare to collect personal data on social and occupational status, family and pathological case history,life style, clinical records, utilisation of welfare and health structure and devices was administered to each patient at basal time and one year after each thermal treatment.Sixty patients with functional dyspepsia and 20 with IBS and 80 healthy controls received an evaluation of gastric output and oro-cecal transit time by breath test analysis.Breath test was performed at basal time and after water supplementaton therapies. Gastrointestinal symptoms were evaluated at the same time points. Breath samples were analyzed with a mass spectometer and a gascromatograph. Results were expressed as T1/2 and T-lag for octanoic acid breath test and as oro-cecal transit time for lactulose breath test.RESULTS: A significant reduction of prevalence of symptoms was observed at the end of the first and second cycles of thermal therapy in dyspeptic and IBS patients.The analysis of variance showed a real and persistant improvement of symptoms in all patients. After water supplementation for 3 wk a reduction of gastric output was observed in 49 (87.5%) of 56 dyspeptic patients.Both T1/2 and T-lag were significantly reduced after the therapy compared to basal values [91± 12 (T1/2) and 53 ± 11 (T-lag), Tables 1 and 2] with results of octanoic acid breath test similar to healthy subjects. After water supplementation for 3 wk oro-cecal transit time was shorter than that at the

  12. Hydrogen sulfide (H2S)/cystathionine γ-lyase (CSE) pathway contributes to the proliferation of hepatoma cells

    Highlights: • Inhibition of H2S/CSE pathway strongly stimulates cellular apoptosis. • Inhibition of H2S/CSE pathway suppresses cell growth by blocking EGFR pathway. • H2S/CSE pathway is critical for maintaining the proliferation of hepatoma cells. - Abstract: Hydrogen sulfide (H2S)/cystathionine γ-lyase (CSE) pathway has been demonstrated to play vital roles in physiology and pathophysiology. However, its role in tumor cell proliferation remains largely unclear. Here we found that CSE over-expressed in hepatoma HepG2 and PLC/PRF/5 cells. Inhibition of endogenous H2S/CSE pathway drastically decreased the proliferation of HepG2 and PLC/PRF/5 cells, and it also enhanced ROS production and mitochondrial disruption, pronounced DNA damage and increased apoptosis. Moreover, this increase of apoptosis was associated with the activation of p53 and p21 accompanied by a decreased ratio of Bcl-2/Bax and up-regulation of phosphorylated c-Jun N-terminal kinase (JNK) and caspase-3 activity. In addition, the negative regulation of cell proliferation by inhibition of H2S/CSE system correlated with the blockage of cell mitogenic and survival signal transduction of epidermal growth factor receptor (EGFR) via down-regulating the extracellular-signal-regulated kinase 1/2 (ERK1/2) activation. These results demonstrate that H2S/CSE and its downstream pathway contribute to the proliferation of hepatoma cells, and inhibition of this pathway strongly suppress the excessive growth of hepatoma cells by stimulating mitochondrial apoptosis and suppressing cell growth signal transduction

  13. Nuclear proliferation and the future of nuclear power

    The author expresses concerns felt worldwide, both within and outside the nuclear industry, that expansion of nuclear power systems will be impossible without safeguards against nuclear weapons proliferation. Present problems include: countries with nuclear programs who are not full participants in the non-proliferation regime; the ease with which countries can withdraw from the treaty; obtaining accurate nuclear material inventories; and compliance. He discusses conditions and improvements that would address many of the proliferation concerns

  14. Inhibitory Effect of Cantharidin on Proliferation of A549 Cells

    WANG Xiao-hua; YIN Yuan-qin; SUI Cheng-guang; MENG Fan-dong; MA Ping; JIANG You-hong

    2007-01-01

    Objective: To study the inhibition of Cantharidin against the proliferation of human lung cancer A549 cells and its mechanism. Methods: MTT assay was employed to determine the inhibition of Cantharidin against proliferation of A549 cells and flow Cytometry was applied to analyze A549 cell cycle and the effect of Cantharidin on cell cycle. Results: Cantharidin showed inhibition against the proliferation of A549 cells, and the inhibition was mediated by blocking A549 cell cycle at G2/M phase significantly. Conclusion: Cantharidin exhibits inhibition against the proliferation of human lung cancer A549 cells.

  15. Assessing the proliferation resistance of innovative nuclear fuel cycles

    Full text: The National Nuclear Security Administration (NNSA) is developing methods for nonproliferation assessments to support the development and implementation of U.S. nonproliferation policy. A Nonproliferation Assessment Methodology (NPAM) Working Group, comprised of representatives from the DOE laboratories and academia, was established to prepare guidelines for the selection of methods and for the performance of nonproliferation assessments. The guidelines address the full scope of proliferation issues that must be addressed by NNSA in support of the development of nonproliferation policy. The guidelines were completed in November 2002 and submitted for peer review. Proliferation is defined as the 'acquisition of one or more nuclear weapons by a nation or subnational group that currently does not have them'. Nonproliferation assessments generally attempt to measure the proliferation resistance of a particular alternative or the proliferation risk of a certain action or proposition. It is important to distinguish between the two types of assessment because they rely on different measures. Proliferation Resistance - Degree of difficulty that a nuclear material, facility, process, or activity poses to the acquisition of one or more nuclear weapons). Proliferation Risk - The likelihood of a nation or subnational group acquiring one or more nuclear weapons within a given time period. Proliferation resistance is an attribute of a nuclear system (a commercial fuel cycle, a facility, transportation of nuclear material, etc.). Proliferation risk, on the other hand, can apply to actions or activities not necessarily part of a physical nuclear system. Acquisition of specific technologies or skills, industrial capabilities, etc., can bear on the risk of proliferation. In comparing the proliferation characteristics of innovative nuclear fuel cycle systems, proliferation resistance is a more appropriate measure of performance than proliferation risk because of the focus

  16. Radon monitoring accompanying rehabilitation of the Schlema/Alberoda pit between 1993 and 1996

    With the closure of the uranium mining industry in Schlema-Alberoda/Saxony extensive measures of reorganization and environmental protection were initiated. From the beginning the rehabilitation of the mining district Schlema was accompanied by an extensive, environmental monitoring of several years. The reason for this work was the documentation of radiological modifications in the Schlema area, in connection with the deactivation and reorganization of the mining industry systems. The radonload of near-surface atmospheric air and in the soil was regularly measured. The total load of radon and its decay products was registered at the same time in the vicinity of Schlema using solid state track detectors. Meteorological data of the local station were available in addition to the radon measurements. With the closure of the ore production and processing in the area Schlema-Alberoda the radiation people of this area were exposed to was reduced continuously. During the measures of reorganization brief extreme exposures were observed. That can be explained by the flooding of the mines as well as the extensive relocation of waste dump material. Meteorologically caused fluctuations of the radon load in the near-surface air layers within the valley of Schlema are comparatively small. (orig.)

  17. [A suspected case of Wegener granulomatosis accompanied with pachymeningitis and white matter lesions].

    Yokoyama, Tomohisa; Abe, Tetsuya; Kurakawa, Eri; Kasuga, Ikuma; Park, Jinho; Akata, Souichi; Aoshima, Masahiro; Ohyashiki, Kazuma

    2005-03-01

    A 53-year-old woman was admitted to our hospital because of high fever and abnormal chest radiograph shadows. Chest X-ray on admission showed a nodular shadow in the right upper lung field and a mass shadow with a cavity in the left middle lung field. Laboratory data indicated leukocytosis and elevation of C-reactive protein. Pulmonary suppuration was suspected, panipenem/betamipron was prescribed, but a mass and consolidation developed, and the medication was changed to ciprofloxacin. Convulsive seizures with loss of consciousness appeared after the change to ciprofloxacin. Lumbar puncture revealed pleocytosis with a predominance of mononuclear cells (198/3) and elevated protein(83 mg/dl). Brain CT showed no abnormal image, and acute aseptic meningitis was diagnosed and was treated with cefotaxime, clindamycin, fluconazole, acicrovir and sulfamethoxazole/trimethoprim. However, the treatment did not result in symptomatic improvement, and brain MRI showed intracranial disorders. Serum PR3-ANCA was elevated to 15 U/ml. Taken together with chest X-ray, sinusitis and clinical course, a generalized form of Wegener's granulomatosis was diagnosed. She was given 60 mg/day of prednisolone, 100 mg/day of cyclophosphamide and 9 g/day of sulfamethoxazole-trimethoprim and progressively improved. In this process, enhanced MR images showed thickened dural enhancement of the falx and bilateral anterior regions, which showed improvement on brain MRI at 8 months after starting treatment. We report a rare case of Wegener's granulomatosis accompanied with pachymeningitis and white matter lesions. PMID:15912757

  18. Presentation of new tectonic map (and accompanying sections) of Trinidad and Tobago

    Persad, K.M.

    1984-04-01

    The Geologic Map of Trinidad, compiled by H.G. Kugler and published in 1961, is currently out of print. While it is the most widely available geologic map, it is confined to onshore Trinidad. This map remains an important reference source, but there have been significant increases in our knowledge of Trindad and Tobago geology since its publication. In particular, there has been: (1) considerable geophysical work, on land and offshore, including 20,000 km (12,400 mi) of seismic lines; (2) approximately 1000 exploration and development wells drilled, including wells in the previously unexplored north and east coast of Trinidad; and (3) significant advances in our understanding of the tectonic evolution of the area, which has resulted largely from the development of the plate tectonic theory. The following items, which take into account many of these new data and concepts, have been compiled. (1) A geologic tectonic map of the entire territory of Trinidad and Tobago, at a scale of 1:200,000. Apart from the surface geology of the land areas, this map shows the major faults and their displacements and locations, total depths and status of exploration wells, and the positions of major petroleum fields. (2) Five accompanying geologic sections at the same scale. (3) A new stratigraphic correlation chart. These new compilations attempt to fill the gap in the published literature on the petroleum geology of Trinidad and Tobago.

  19. Damped summer warming accompanied with cloud cover increase over Eurasia from 1982 to 2009

    The relationship between summer temperature, total cloud cover and precipitation over Eurasia was investigated using observation-based products of temperature and precipitation, and satellite-derived cloud cover and radiation products. We used a partial least squares regression approach to separate the local influences of cloud cover and precipitation on temperature variations. Our results suggest that the variance of summer temperature is partly explained by changes in summer cloudiness. The summer temperature dependence on cloud cover is strong at the high latitudes and in the middle latitude semi-humid area, while the dependence on precipitation is strong in the Central Asia arid area and the southern Asia humid area. During the period 1982–2009, the damped warming in extended West Siberia was accompanied with increases in cloud cover, and the pronounced warming in Europe and Mongolia was associated with a decrease in cloud cover and precipitation. Our results suggest that cloud cover may be the important local factor influencing the summer temperature variation in Eurasia while precipitation plays an important role at the middle latitudes. (letter)

  20. Frontal and temporal contributions to understanding the iconic co-speech gestures that accompany speech.

    Dick, Anthony Steven; Mok, Eva H; Raja Beharelle, Anjali; Goldin-Meadow, Susan; Small, Steven L

    2014-03-01

    In everyday conversation, listeners often rely on a speaker's gestures to clarify any ambiguities in the verbal message. Using fMRI during naturalistic story comprehension, we examined which brain regions in the listener are sensitive to speakers' iconic gestures. We focused on iconic gestures that contribute information not found in the speaker's talk, compared with those that convey information redundant with the speaker's talk. We found that three regions-left inferior frontal gyrus triangular (IFGTr) and opercular (IFGOp) portions, and left posterior middle temporal gyrus (MTGp)--responded more strongly when gestures added information to nonspecific language, compared with when they conveyed the same information in more specific language; in other words, when gesture disambiguated speech as opposed to reinforced it. An increased BOLD response was not found in these regions when the nonspecific language was produced without gesture, suggesting that IFGTr, IFGOp, and MTGp are involved in integrating semantic information across gesture and speech. In addition, we found that activity in the posterior superior temporal sulcus (STSp), previously thought to be involved in gesture-speech integration, was not sensitive to the gesture-speech relation. Together, these findings clarify the neurobiology of gesture-speech integration and contribute to an emerging picture of how listeners glean meaning from gestures that accompany speech. PMID:23238964

  1. LATE-TIME OBSERVATIONS OF GRB 080319B: JET BREAK, HOST GALAXY, AND ACCOMPANYING SUPERNOVA

    The Swift-discovered GRB 080319B was by far the most distant source ever observed at naked-eye brightness, reaching a peak apparent magnitude of 5.3 at a redshift of z = 0.937. We present our late-time optical (Hubble Space Telescope, Gemini, and Very Large Telescope) and X-ray (Chandra) observations, which confirm that an achromatic break occurred in the power-law afterglow light curve at ∼11 days post-burst. This most likely indicates that the gamma-ray burst (GRB) outflow was collimated, which for a uniform jet would imply a total energy in the jet Ejet ∼> 1052 erg. Our observations also show a late-time excess of red light, which is well explained if the GRB was accompanied by a supernova (SN), similar to those seen in some other long-duration GRBs. The latest observations are dominated by light from the host and show that the GRB took place in a faint dwarf galaxy (r(AB) ∼ 27.0, rest frame MB ∼ -17.2). This galaxy is small even by the standards of other GRB hosts, which is suggestive of a low-metallicity environment. Intriguingly, the properties of this extreme event-a small host and bright SN-are entirely typical of the very low luminosity bursts such as GRB 980425 and GRB 060218.

  2. Multiple Fractures in Patient with Graves' Disease Accompanied by Isolated Hypogonadotropic Hypogonadism.

    Yi, Hyon-Seung; Kim, Ji Min; Ju, Sang Hyeon; Lee, Younghak; Kim, Hyun Jin; Kim, Koon Soon

    2016-02-01

    Isolated hypogonadotropic hypogonadism (IHH) is known to decrease bone mineral density due to deficiency of sex steroid hormone. Graves' disease is also an important cause of secondary osteoporosis. However, IHH does not preclude the development of primary hyperthyroidism caused by Graves' disease, leading to more severe osteoporosis rapidly. Here, we describe the first case of 35-year-old Asian female patient with IHH accompanied by Graves' disease and osteoporosis-induced multiple fractures. Endocrine laboratory findings revealed preserved anterior pituitary functions except for secretion of gonadotropins and showed primary hyperthyroidism with positive autoantibodies. Sella magnetic resonance imaging showed slightly small sized pituitary gland without mass lesion. Dual energy X-ray absorptiometry revealed severe osteoporosis in lumbar spine and femur neck of the patient. Plain film radiography of the pelvis and shoulder revealed a displaced and nondisplaced fracture, respectively. After surgical fixation with screws for the femoral fracture, the patient was treated with antithyroid medication, calcium, and vitamin D until now and has been recovering fairly well. We report a patient of IHH with Graves' disease and multiple fractures that is a first case in Korea. PMID:26981520

  3. Sweet Syndrome Accompanying Inflammatory Dehydrogenase Deficiency and Its Association Bowel Disease in a Child

    Anahita Sanaei Dashti

    2010-03-01

    Full Text Available Acute neutrophilic dermatosis, first described in 1964 byRobert Douglas Sweet, is characterized by sudden onset fever,neutrophilic leukocytosis, and well demarcated erythematouspapules, nodules, and plaques with dense neutrophilic infiltrateson histologic evaluation.Here is a report of a 7-year-old girl who presented withhigh grade fever, and discrete erythematous papular skin eruptions,which gradually increased in number and involved theface, trunk, extremities, palms, soles, hard palate, and palataltonsils. The skin eruptions evolved to pustules and after coalescingcaused large crusted plaques, with mild tenderness butwithout any pruritus. White blood cells were 36900/ml with92% neutrophils. Skin biopsy test was compatible with acutefebrile neutrophilic dermatosis, so prednisolone (1 mg/kg/daywas started that led to a rapid defervescence and significantimprovement of dermatosis. After a few days, the patient presentedwith fever and arthritis of right elbow, both ankles, andwrists, so she was re-admitted. She also developed bloody diarrheaduring the hospital stay. Colonoscopy and intestinalbiopsy were performed, which confirmed the diagnosis of ulcerativecolitis. Prednisolone, sulfasalazine, and naproxenwere prescribed. The fever and diarrhea stopped after a fewdays and joint swelling decreased. She was discharged 2weeks after the admission with a rather good general condition.Inflammatory bowel disease can be one of the severalconditions accompanying sweet syndrome.

  4. Demands, subjectivity and therapeutic process: construction and limitations of Therapeutic Accompaniment

    Luís Felipe Ferro

    2014-12-01

    Full Text Available The Brazilian Psychiatric Reform movement proposed several changes in the field of mental health. Since then, guidelines have been redirecting actions to respond to different demands, associating social inclusion with clinical follow-up. In this context, Therapeutic Accompaniment (TA appears as an important device to compose interventions focused on the demands of individuals with mental disorders. Thus the present work aims to present reflections on the limitations and potentials of TA. This study was based on the practice of TA in the Occupational Therapy Traineeship in Mental Health of the Federal University of Parana - UFPR. Regarding the methodological framework, this paper is structured in a two-way approach: presentation of a case report and exploratory research using unstructured interviews focused on two subjects monitored by TA. Hermeneutic-dialectic analysis was used for data treatment. The results indicate that TA provides significant gains to the process of rehabilitation and construction of citizenship of individuals with mental disorders, enabling actions focused on the demands of the individual, family and community. Nevertheless, there is still the need for continuity of TA interventions, as well as some structural deadlocks that tend to decrease the power of this device.

  5. Application of near-infrared spectroscopy to measurement of hemodynamic signals accompanying stimulated saliva secretion.

    Sato, Hiroki; Obata, Akiko N; Moda, Ichiro; Ozaki, Kazutaka; Yasuhara, Takaomi; Yamamoto, Yukari; Kiguchi, Masashi; Maki, Atsushi; Kubota, Kisou; Koizumi, Hideaki

    2011-04-01

    We aim to test the feasibility of using near-infrared spectroscopy (NIRS) for indirect measurement of human saliva secretion in response to taste stimuli for potential application to organoleptic testing. We use an NIRS system to measure extracranial hemodynamics (Hb-signals around the temples) of healthy participants when taste stimuli are taken in their mouths. First, the Hb-signals and volume of expelled saliva (stimulated by distilled-water or sucrose-solution intake) are simultaneously measured and large Hb-signal changes in response to the taste stimuli (Hb-responses) are found. Statistical analysis show that both the Hb response and saliva volume are larger for the sucrose solution than for the distilled water with a significant correlation between them (r = 0.81). The effects of swallowing on the Hb-signals are investigated. Similar Hb responses, differing from the sucrose solution and distilled water, are obtained even though the participants swallow the mouth contents. Finally, functional magnetic resonance imaging is used to identify possible sources of the Hb signals corresponding to salivation. Statistical analysis indicates similar responses in the extracranial regions, mainly around the middle meningeal artery. In conclusion, the identified correlation between extracranial hemodynamics and the saliva volume suggests that NIRS is applicable to the measurement of hemodynamic signals accompanying stimulated saliva secretion. PMID:21529092

  6. GRB off-axis afterglows and the emission from the accompanying supernovae

    Kathirgamaraju, Adithan; Giannios, Dimitrios

    2016-01-01

    Gamma-Ray Burst (GRB) afterglows are likely produced in the shock that is driven as the GRB jet interacts with the external medium. Long duration GRBs are also associated with powerful supernovae (SN). We consider the optical and radio afterglows of long GRBs for both blasts viewed along the jet axis ("on-axis" afterglows) and misaligned observes ("off-axis" afterglows). Comparing the optical emission from the afterglow with that of the accompanying SN, using SN 1998bw as an archetype, we find that only a few percent of afterglows viewed off-axis are brighter than the SN. For observable optical off-axis afterglows the viewing angle is at most twice the half-opening angle of the GRB jet. Radio off-axis afterglows should be detected with upcoming radio surveys within a few hundred Mpc. We propose that these surveys will act as "radio triggers," and that dedicated radio facilities should follow-up these sources. Follow-ups can unveil the presence of the radio supernova remnant, if present. In addition, they can ...

  7. Autophagy Accompanied with Bisdemethoxycurcumin-induced Apoptosis in Non-small Cell Lung Cancer Cells

    XU Jin Hong; YANG He Ping; ZHOU Xiang Dong; WANG Hai Jing; GONG Liang; TANG Chun Lan

    2015-01-01

    Objective To investigate the effects of bisdemethoxycurcumin (BDMC) on non-small cell lung cancer (NSCLC) cell line, A549, and the highly metastatic lung cancer 95D cells. Methods CCK-8 assay was used to assess the effect of BDMC on cytotoxicity. Flow cytometry was used to evaluate apoptosis. Western blot analysis, electron microscopy, and quantification of GFP-LC3 punctuates were used to test the effect of BDMC on autophagy and apoptosis of lung cancer cells. Results BDMC inhibited the viability of NSCLC cells, but had no cytotoxic effects on lung small airway epithelial cells (SAECs). The apoptotic cell death induced by BDMC was accompanied with the induction of autophagy in NSCLC cells. Blockage of autophagy by the autophagy inhibitor 3-methyladenine (3-MA) repressed the growth inhibitory effects and induction of apoptosis by BDMC. In addition, BDMC treatment significantly decreased smoothened (SMO) and the transcription factor glioma-associated oncogene 1 (Gli1) expression. Furthermore, depletion of Gli1 by siRNA and cyclopamine (a specific SMO inhibitor) induced autophagy. Conclusion Aberrant activation of Hedgehog (Hh) signaling has been implicated in several human cancers, including lung cancers. The present findings provide direct evidence that BDMC-induced autophagy plays a pro-death role in NSCLC, in part, by inhibiting Hedgehog signaling.

  8. Prediction of forced gas flows in circular tubes at high heat fluxes accompanied by laminarization

    The application of high heat fluxes to turbulent gas flows causes significant variation of the gas properties due to the large temperature increase, invalidating the use of design relations such as the popular Dittus-Boelter correlation and of conventional turbulence models. To develop turbulence models for such flows, the predictive capabilities of a Reynolds stress equation model (RSM) with turbulence heat flux equations and of a k-kL-(u'v')-bar model were examined. We have employed the turbulence stress-thermal energy gradient production, in the pressure-temperature fluctuation gradient correlation, for the turbulence heat flux equations, in order to improve the accuracy of predictions for heat transfer accompanied by laminarization. The k-kL-(u'v')-bar model has already been validated for gas flows in annular tubes; in this study its applicability to a circular tube was examined. Validations were performed by comparison of predictions with constant fluid properties at fully-established conditions and to experimental measurements extending to laminarization conditions. The turbulence models both predict laminar and turbulent results and the approximate transition Reynolds number for the fully-established flow at low heat fluxes. The results predicted with both models also agreed well with data on laminarizing flows. Thus, the models presented here are capable of predicting the flow reasonably from low to high heat fluxes. (author)

  9. Formation of electrical field accompanying temperature jump in isolated spinach chloroplasts.

    Shimizu, M; Nishimura, M

    1977-03-11

    Temperature-jump-induced absorbance changes of spinach chloroplasts in the dark were studied. After the temperature rise, a fast absorbance decrease and a succeeding slow absorbance increase were observed at the wavelength of 515 nm. The spectrum of the fast phase had positive maxima (increase in absorbance) at 430, 470 and 673 nm and a negative maxima (decrease in absorbance) at 525 nm. Permeant ions, tetraphenylboron-, tetraphenylarsonium+, and tetraphenylphosphonium+, decreased the extent of the fast absorbance change and increased the rate of slow recovery. Additions of inorganic potassium salts had a similar effect. Valinomycin, added in the presence of potassium ion, also increased the rate of slow recovery. These ions and ionophore had a parallel effect also on the recovery of flash-induced 515-nm absorbance change in chloroplasts. Electroneutral nigerericin did not affect the temperature-jump-induced absorbanc change. These results suggest the formation of electrical field across the thylakoid membrane in the dark accompanying the temperature rise. A possible involvement of the movement of water molecules (thermo-osmosis) in the observed absorbance changes is also discussed. PMID:849433

  10. Long-term salinity tolerance is accompanied by major restructuring of the coral bacterial microbiome

    Rothig, Till

    2016-02-03

    Scleractinian corals are assumed to be stenohaline osmoconformers, although they are frequently subjected to variations in seawater salinity due to precipitation, freshwater runoff, and other processes. Observed responses to altered salinity levels include differences in photosynthetic performance, respiration, and increased bleaching and mortality of the coral host and its algal symbiont, but a study looking at bacterial community changes is lacking. Here we exposed the coral Fungia granulosa to strongly increased salinity levels in short- and long-term experiments to disentangle temporal and compartment effects of the coral holobiont (i.e. coral host, symbiotic algae, and associated bacteria). Our results show a significant reduction in calcification and photosynthesis, but a stable microbiome after short-term exposure to high salinity levels. By comparison, long-term exposure yielded unchanged photosynthesis levels and visually healthy coral colonies indicating long-term acclimation to high salinity levels that were accompanied by a major coral microbiome restructuring. Importantly, a bacterium in the family Rhodobacteraceae was succeeded by Pseudomonas veronii as the numerically most abundant taxon. Further, taxonomy-based functional profiling indicates a shift in the bacterial community towards increased osmolyte production, sulfur oxidation, and nitrogen fixation. Our study highlights that bacterial community composition in corals can change within days to weeks under altered environmental conditions, where shifts in the microbiome may enable adjustment of the coral to a more advantageous holobiont composition.

  11. Chemical transition of nitrate ions accompanied with corrosion of carbon steel under alkaline conditions

    Carbon steel was immersed in an aqueous solution of NaNO3 in a closed system for observing both the chemical interaction between metal and NO3-, and the effect of nitrate on the generation rate of H2 gas. The experimental pH range of the solution was 10.0-13.5 which corresponds to that of pore fluid in cementitious material. The cathodic current density showed a 'Tafel equation type' potential dependency in aqueous solution containing NO3- or NO2-. In spite of the accelerated cathodic reaction due to the existence of nitrate, the corrosion rate of carbon steel was not accelerated in the nitrate solutions. This fact suggests that the system is controlled by the anodic reaction. The nitrate reduction accompanied by the corrosion of carbon steel is considered to be a series reaction such as NO3-→NO2-→NH3. The nitrate reduction reaction competes with the water reduction reaction (hydrogen evolution reaction) within the anodic controlled condition, therefore nitrate strongly reduces the hydrogen generation rate (1/100-1/500 of the cases without nitrate in 1.0 mol dm-3 NaNO3 cases). The generation rates of NH3 were independent of the concentration of NO3- over the range of 1.0 x 10-3 - 1.0 mol dm-3. (author)

  12. "El Sexo no es Malo": Maternal Values Accompanying Contraceptive Use Advice to Young Latina Adolescent Daughters.

    Romo, Laura F; Bravo, Magali; Cruz, Maria Elena; Rios, Rebeca M; Kouyoumdjian, Claudia

    2010-06-01

    In this study, we utilized observational methods to identify maternal values and concerns accompanying contraceptive use advice in Latina mother-daughter sexuality conversations. The sample included non-sexually active early adolescents around 12 years of age and their mostly Spanish-speaking Latina mothers. Videotaped conversations were coded for the prevalence of messages related to four sexual values (abstinence, delay sex until older, sex is "normal", sex is "improper") and concerns about pregnancy and STD transmission. We examined whether the duration of time spent conversing about these messages was associated with participant characteristics, general communication openness, and the amount of time the dyads spent discussing contraceptive use. Results indicated that Latina mothers who had fewer years of education and lower family income talked longer to their daughters about the need to delay sex, avoid risky situations that would increase their chances of getting pregnant or acquiring an STD, and engage in self-protective practices. Less perceived openness in general communication as reported by both the mothers and the daughters was associated with increased time discussing that sex is improper. Although the duration of contraceptive use messages was brief, mothers and daughters who discussed the fact that sex is normal, and who communicated more about the importance of delaying sex, talked longer about contraceptive use practices compared to mothers and daughters who engaged in minimal discussion of these sexual values. PMID:20543876

  13. LUCC and Accompanied Soil Degradation in China from 1960's to 1990's

    JIANG Qigang; Shintaro GOTO; Hiroki TAKAMURA

    2002-01-01

    This paper tries to qualitatively analyze land use and cover changes (LUCC) and accompanied soil degradation in China, the data of World Atlas of Agriculture in 1969, Land Cover Data of Asia in 1992 and Global Assessment of Human Induced Soil Degradation in 1992, etc. have been used. From 1960' s to 1990's, the area of forestland had increased, arable land and paddy as well as grassland had significantly decreased in China. The major type of soil degradation is due to water erosion, which is widely distributed especially in Loess Plateau and in Southeast and Southwest China, this happened in forestland, grassland and arable land and mainly resulted from deforestation. The secondary type is wind erosion, which is mainly distributed in Northwest China, it happened in arable land and grassland and was caused by overgrazing. Chemical deterioration is distributed in North and Northwest China, which happened in paddy, arable land and grassland. Physical deterioration only concentrated in local area in North China, it happened in arable and paddy. Chemical and physical deteriora-tion mainly resulted from unreasonable activity. Because of different human activities, different LUCC and different natural conditions, the types and strength of soil degradation can be different.

  14. Pseudoaneurysm Accompanied by Crowe Type IV Developmental Dysplasia of the Hip: A Case Report

    Hirotake Yo

    2012-01-01

    Full Text Available We report the case of a 72-year-old woman whose pseudoaneurysm was difficult to diagnose and treat. The patient had a history of congenital dislocated hip and was undergoing anticoagulation therapy with warfarin due to the mitral valve replacement. Her chief complaint was pain and enlargement of the left buttock, and the laboratory tests revealed severe anemia. However, her elderly depression confused her chief complaint, and she was transferred to a psychiatric hospital. Two months after the onset of the symptoms, she was finally diagnosed with a pseudoaneurysm by contrast-enhanced CT and angiography. IDC coils were used for embolization. A plain CT showed hemostasis as well as a reduced hematoma at 2 months after the embolization. The possible contributing factors for the pseudoaneurysm included bleeding due to warfarin combined with an intramuscular hematoma accompanied by Crowe type IV developmental dysplasia of the hip that led to an arterial rupture by impingement between pelvis and femoral head. Since the warfarin treatment could not be halted due to the valve replacement, embolization was chosen for her treatment, and the treatment outcome was favorable.

  15. High levels of impulsivity in rats are not accompanied by sensorimotor gating deficits and locomotor hyperactivity.

    Feja, M; Lang, M; Deppermann, L; Yüksel, A; Wischhof, L

    2015-12-01

    High levels of impulsivity have been linked to a number of psychiatric disorders, including attention-deficit/hyperactivity disorder, drug abuse and schizophrenia. Additionally, schizophrenia patients commonly show deficits in another rather preattentive form of response inhibition, called sensorimotor gating. Given that higher-order functions, such as impulse control, are protected by early and preattentive processes, disturbed gating mechanisms may hamper more complex cognitive-executive functions. In the present study, we therefore tested whether high levels of impulsivity are accompanied by impaired sensorimotor gating in rats. High (HI) and low impulsive (LI) rats were identified based on the number of premature responses in the 5-choice serial reaction time task. Here, LI rats showed higher numbers of omission errors which may suggest attentional deficits while HI rats completed significantly less trials which could indicate a decrease in motivation. However, HI and LI rats did not differ in terms of impulsive decision-making in a delay-based decision-making T-maze task, prepulse inhibition of the acoustic startle response (a measure of sensorimotor gating mechanisms) or locomotor activity levels. Overall, our data indicate that high motor impulsivity is not a suitable predictor of deficient sensorimotor gating and is further not necessarily associated with attentional deficits and/or locomotor hyperactivity in rats. PMID:26484709

  16. A fatal case of severe serotonin syndrome accompanied by moclobemide and paroxetine overdose

    Sener Serkan

    2005-01-01

    Full Text Available Aim: To present a fatal case of serotonin syndrome accompanied by moclobemide and paroxetine overdose. Case presentation: A 34-year-old married woman was presented following intentional ingestion of 3.5 g moclobemide and 2.6 g paroxetine. She was drowsy, agitated, and having rigor. In 1 h she developed myoclonus and diffuse muscle rigidity prominent in lower extremities. All laboratory tests were unremarkable except hyperglycemia (160 mg/dl, sinus tachycardia (103/min, and metabolic acidosis (7.051 pH, 52 mmHg pO2, 74.7 mmHg pCO2, 15% HCO3, 77% SaO2. Despite oxygen supplementation, her respiratory acidosis got worse and the SaO2 concentration decreased to 72%. Endotracheal intubation and paralysis were decided to control muscle hyperactivity followed by hyperthermia (max. 42.3°C unresponsive to benzodiazepine. Even aggressive supportive treatment (mechanical ventilation, buffer replacement, cyproheptadine, and dantrolene were applied, the patient could not recover and suffered cardiopulmonary arrest 20 h after presentation. Conclusion: Physicians working in the emergency departments and intensive care units, managing patients presenting with acute ingestion of selective serotonin reuptake inhibitors combined with monoamine oxidase inhibitors, should be aware of recognizing and treating serotonin stndrome. This is because many of these patients may require intensive care monitoring as well as tracheal intubation and ventilatory support.

  17. Risk Factors Accompanied with Nephropathy in Patients with Type II Diabetes; Test of the Biopsychosocial Model

    I. Rahimian Boogar

    2012-07-01

    Full Text Available Introduction & Objective: The study of biopsychosocial factors influencing nephropathy as a most serious complication of type II diabetes is important. This study aimed to investigate risk factors accompanied with nephropathy in patients with type II diabetes based on the biopsychosocial model. Materials & Methods: In a cross-sectional descriptive study, 295 patients with type II diabetes were selected by convenience sampling in Tehran Shariati hospital outpatient clinics. The data were collected by demographical information questionnaire along with disease characteristics and depression anxiety stress scales (dass, quality of life scale (who- qol- bref, diabetes self-management scale (dsms, and diabetes knowledge scale (dks, then analyzed by chi-square, independent t-test and logistic regression with pasw software. Results: Hypertension (OR=3.841 & P0.05.Conclusion: It is important to pay attention to hypertension, glycated hemoglobin, body mass index, diabetes self-management, depression, quality of life, and diabetes knowledge for therapeutic intervention programming and diabetes complications control protocols for diabetic patients.(Sci J Hamadan Univ Med Sci 2012;19(2:44-53

  18. Research on radiation safety accompanying transport of low level radioactive substances

    This research aims at completing the code system that can evaluate in detail the radiation exposure in the sea transport of low level waste, and enabling the estimation of the safety margin of radiation shields. In fiscal year 1995, the second experiment on an actual ship and its analysis, and the evaluation of safety margin accompanying the modeling of the computed form were carried out. The LLW carrier ''Seiei-maru'' of about 100 m length and 3000 t deadweight has 7 holds, in which 384 containers or 3072 drums can be loaded. In the transport of this time, 360 containers were transported, and the radioactivity in respective holds is shown. The nuclides related to external exposure are 60Co and 137Cs. The dose measurement was carried out with the TCS-161 survey meter made by ALOKA Co. The maximum dose on hatch covers and in living section was far under the values of regulation, and it was able to be confirmed that the safety regarding the radiation exposure of crews at the time of this transport has been secured. The dose rate distribution on hatch covers was analyzed by using the continuous energy Monte Carlo code. MCNP 4A. The effect that the difference of modeling exerts to the result of calculation was examined. The results are reported. (K.I.)

  19. Similarity dependency of the change in ERP component N1 accompanying with the object recognition learning.

    Tokudome, Wataru; Wang, Gang

    2012-01-01

    Performance during object recognition across views is largely dependent on inter-object similarity. The present study was designed to investigate the similarity dependency of object recognition learning on the changes in ERP component N1. Human subjects were asked to train themselves to recognize novel objects with different inter-object similarity by performing object recognition tasks. During the tasks, images of an object had to be discriminated from the images of other objects irrespective of the viewpoint. When objects had a high inter-object similarity, the ERP component, N1 exhibited a significant increase in both the amplitude and the latency variation across objects during the object recognition learning process, and the N1 amplitude and latency variation across the views of the same objects decreased significantly. In contrast, no significant changes were found during the learning process when using objects with low inter-object similarity. The present findings demonstrate that the changes in the variation of N1 that accompany the object recognition learning process are dependent upon the inter-object similarity and imply that there is a difference in the neuronal representation for object recognition when using objects with high and low inter-object similarity. PMID:22115890

  20. Chaos in oscillatory heat evolution accompanying the sorption of hydrogen and deuterium in palladium

    Lalik, Erwin

    2012-01-01

    Aperiodic oscillations in the sorption of H2 or D2 in metallic Pd powder have been observed, and a novel method to confirm their deterministic rather than random character has been devised. A theorem relating the square of a function, with the derivative and integral with variable upper limit of the same function has been proved and proposed to be used as a base for a chaos-vs-random test. Both the experimental and the computed time series may be tested to detect determinism. The result is a single number within the interval [0,2]. The test is designed in such a way that its result is close to zero for the datasets that are deterministic and smooth, and close to 2 for the datasets that are non deterministic (random) or non smooth (discrete). A large variety of the test results has been obtained for the calorimetric time series recorded in thermokinetic oscillations, periodic and quasiperiodic, accompanying the sorption of H2 or D2 with Pd as well as for several non oscillatory calorimetric curves recorded in ...

  1. Long-term salinity tolerance is accompanied by major restructuring of the coral bacterial microbiome.

    Röthig, Till; Ochsenkühn, Michael A; Roik, Anna; van der Merwe, Riaan; Voolstra, Christian R

    2016-03-01

    Scleractinian corals are assumed to be stenohaline osmoconformers, although they are frequently subjected to variations in seawater salinity due to precipitation, freshwater run-off and other processes. Observed responses to altered salinity levels include differences in photosynthetic performance, respiration and increased bleaching and mortality of the coral host and its algal symbiont, but a study looking at bacterial community changes is lacking. Here, we exposed the coral Fungia granulosa to strongly increased salinity levels in short- and long-term experiments to disentangle temporal and compartment effects of the coral holobiont (i.e. coral host, symbiotic algae and associated bacteria). Our results show a significant reduction in calcification and photosynthesis, but a stable microbiome after short-term exposure to high-salinity levels. By comparison, long-term exposure yielded unchanged photosynthesis levels and visually healthy coral colonies indicating long-term acclimation to high-salinity levels that were accompanied by a major coral microbiome restructuring. Importantly, a bacterium in the family Rhodobacteraceae was succeeded by Pseudomonas veronii as the numerically most abundant taxon. Further, taxonomy-based functional profiling indicates a shift in the bacterial community towards increased osmolyte production, sulphur oxidation and nitrogen fixation. Our study highlights that bacterial community composition in corals can change within days to weeks under altered environmental conditions, where shifts in the microbiome may enable adjustment of the coral to a more advantageous holobiont composition. PMID:26840035

  2. Polyploid formation in cotton is not accompanied by rapid genomic changes.

    Liu, B; Brubaker, C L; Mergeai, G; Cronn, R C; Wendel, J F

    2001-06-01

    Recent work has demonstrated that allopolyploid speciation in plants may be associated with non-Mendelian genomic changes in the early generations following polyploid synthesis. To address the question of whether rapid genomic changes also occur in allopolyploid cotton (Gossypium) species, amplified fragment length polymorphism (AFLP) analysis was performed to evaluate nine sets of newly synthesized allotetraploid and allohexaploid plants, their parents, and the selfed progeny from colchicine-doubled synthetics. Using both methylation-sensitive and methylation-insensitive enzymes, the extent of fragment additivity in newly combined genomes was ascertained for a total of approximately 22,000 genomic loci. Fragment additivity was observed in nearly all cases, with the few exceptions most likely reflecting parental heterozygosity or experimental error. In addition, genomic Southern analysis on six sets of synthetic allopolyploids probed with five retrotransposons also revealed complete additivity. Because no alterations were observed using methylation-sensitive isoschizomers, epigenetic changes following polyploid synthesis were also minimal. These indications of genomic additivity and epigenetic stasis during allopolyploid formation provide a contrast to recent evidence from several model plant allopolyploids, most notably wheat and Brassica, where rapid and unexplained genomic changes have been reported. In addition, the data contrast with evidence from repetitive DNAs in Gossypium, some of which are subject to non-Mendelian molecular evolutionary phenomena in extant polyploids. These contrasts indicate polyploid speciation in plants is accompanied by a diverse array of molecular evolutionary phenomena, which will vary among both genomic constituents and taxa. PMID:11444689

  3. Neuromodulation of Limb Proprioceptive Afferents Decreases Apnea of Prematurity and Accompanying Intermittent Hypoxia and Bradycardia

    Kesavan, Kalpashri; Frank, Paul; Cordero, Daniella M.; Benharash, Peyman; Harper, Ronald M.

    2016-01-01

    Background Apnea of Prematurity (AOP) is common, affecting the majority of infants born at prematurity (ROP), injury to sympathetic ganglia regulating cardiovascular action, impaired pancreatic islet cell and bone development, cerebellar injury, and neurodevelopmental disabilities. Current standard of care for AOP/IH includes prone positioning, positive pressure ventilation, and methylxanthine therapy; these interventions are inadequate, and not optimal for early development. Objective The objective is to support breathing in premature infants by using a simple, non-invasive vibratory device placed over limb proprioceptor fibers, an intervention using the principle that limb movements trigger reflexive facilitation of breathing. Methods Premature infants (23–34 wks gestational age), with clinical evidence of AOP/IH episodes were enrolled 1 week after birth. Caffeine treatment was not a reason for exclusion. Small vibration devices were placed on one hand and one foot and activated in 6 hour ON/OFF sequences for a total of 24 hours. Heart rate, respiratory rate, oxygen saturation (SpO2), and breathing pauses were continuously collected. Results Fewer respiratory pauses occurred during vibration periods, relative to baseline (ppremature neonates, limb proprioceptive stimulation, simulating limb movement, reduces breathing pauses and IH episodes, and lowers the number of bradycardic events that accompany aberrant breathing episodes. This low-cost neuromodulatory procedure has the potential to provide a non-invasive intervention to reduce apnea, bradycardia and intermittent hypoxia in premature neonates. Trial Registration ClinicalTrials.gov NCT02641249 PMID:27304988

  4. The β-carboline alkaloid harmine inhibits telomerase activity of MCF-7 cells by down-regulating hTERT mRNA expression accompanied by an accelerated senescent phenotype

    Lei Zhao

    2013-10-01

    Full Text Available The end replication problem, which occurs in normal somatic cells inducing replicative senescence, is solved in most cancer cells by activating telomerase. The activity of telomerase is highly associated with carcinogenesis which makes the enzyme an attractive biomarker in cancer diagnosis and treatment. The indole alkaloid harmine has multiple pharmacological properties including DNA intercalation which can lead to frame shift mutations. In this study, harmine was applied to human breast cancer MCF-7 cells. Its activity towards telomerase was analyzed by utilizing the telomeric repeat amplification protocol (TRAP. Our data indicate that harmine exhibits a pronounced cytotoxicity and induces an anti-proliferation state in MCF-7 cells which is accompanied by a significant inhibition of telomerase activity and an induction of an accelerated senescence phenotype by over-expressing elements of the p53/p21 pathway.

  5. Defined nuclear changes accompany the reprogramming of the microspore to embryogenesis.

    Testillano, P S; Coronado, M J; Seguí, J M; Domenech, J; González-Melendi, P; Raska, I; Risueño, M C

    2000-04-01

    The switch of the gametophytic developmental program toward pollen embryogenesis to form a haploid plant represents an important alternative for plant breeding. In the present study, the switch of the gametophytic developmental program toward a sporophytic pathway, "embryogenesis," has been studied in three different plant species, Brassica, tobacco, and pepper. The switch has been induced by stress (heat shock) at the very responsive stage of the microspore, which is the vacuolate period. As a result, the cell nucleus undergoes striking structural changes with regard to late gametophytic development, including alterations of biosynthetic activities and proliferative activity. An enrichment in HSP70 heat-shock protein and in the presence of Ntf6-MAP kinase was observed after inductive treatment in the nuclei during early embryogenesis. This apparently reflected the possible roles of these proteins, specifically the protective role of HSP70 for the nuclear machinery, and signal transduction of Ntf6-MAPK for the entry of cells into proliferation. Importantly, the observed nuclear changes were similar in the three species investigated and represented convenient markers for early monitoring of embryogenesis and selection purposes for obtaining double-haploid plants in plant breeding. PMID:10806072

  6. Modeling and evaluating proliferation resistance of nuclear energy systems for strategy switching proliferation

    Highlights: ► Sensitivity analysis is carried out for the model and physical input parameters. ► Interphase drag has minor effect on the dryout heat flux (DHF) in 1D configuration. ► Model calibration on pressure drop experiments fails to improve prediction of DHF. ► Calibrated classical model provides the best agreement with DHF data from 1D tests. ► Further validation of drag models requires data from 2D and 3D experiments on DHF. - Abstract: This paper reports a Markov model based approach to systematically evaluating the proliferation resistance (PR) of nuclear energy systems (NESs). The focus of the study is on the development of the Markov models for a class of complex PR scenarios, i.e., mixed covert/overt strategy switching proliferation, for NESs with two modes of material flow, batch and continuous. In particular, a set of diversion and/or breakout scenarios and covert/overt misuse scenarios are studied in detail for an Example Sodium Fast Reactor (ESFR) system. Both probabilistic and deterministic PR measures are calculated using a software tool that implements the proposed approach and can be used to quantitatively compare proliferation resistant characteristics of different scenarios for a given NES, according to the computed PR measures

  7. Phenobarbital-induced hepatocellular proliferation: anti-bromodeoxyuridine and anti-proliferating cell nuclear antigen immunocytochemistry.

    Jones, H B; Clarke, N A; Barrass, N C

    1993-01-01

    We report modifications to immunocytochemical detection procedures for proliferating cell nuclear antigen (PCNA) which permit its identification in liver samples previously fixed for BrdU immunocytochemistry. Both methods have been used for the assessment of phenobarbital-induced cell proliferation in rat liver. The difficulties associated with the hitherto unsuccessful application of PCNA immunocytochemical methods to tissues fixed in formalin for BrdU visualization were overcome by epitope unmasking with acid hydrolysis, extension of primary antiserum (PC10) incubation, and employment of streptavidin-ABC-HRP. BrdU delivery via osmotic minipumps for 48 hr before euthanasia, followed by fixation in cold formalin for 14 days, yielded reliable and reproducible hepatocellular labeling and a peak of cell proliferation in all lobes on Day 3 (i.e., labeling during Days 1-3) of dosing with 80 mg/kg/day phenobarbital. Labeling indices (LI) of both control and phenobarbital-treated liver were lower in the left and right median lobes as compared with the lateral lobes. In sections of the left lateral lobe from the same liver, PCNA immunocytochemistry revealed a peak of proliferative activity (about one third of the maximum LI generated by BrdU incorporation) on Day 1. These findings, together with the advantages and disadvantages of both techniques, are discussed in the context of their applications to different investigative requirements. PMID:8093255

  8. A Brief Analysis on Dance Piano Accompaniment Teaching%舞蹈钢琴伴奏教学浅析

    冷平

    2011-01-01

    The essential point of the paper is trying to explore the relationship between dance and music,the position of piano accompaniment in dance performance,how dance moves and piano accompaniment adapt to music styles,and how music sense and artistic accomplishment play important role on promoting dance piano accompaniment and so on.Dance piano accompaniment is not subordinate to dance.On the contrary,piano accompaniment can strengthen the dance emotion and endow with movement and vitality in the classroom teaching activities.Besides,it can make students more easily understand the content of dance training and improve dance teaching quality.%舞蹈与音乐是相生相灭、相辅相成的.钢琴伴奏是当今舞蹈教学中不可缺少的环节,是舞蹈教学中的重要组成部分.充分发挥钢琴伴奏在舞蹈教学中的作用,达到音乐与舞蹈完美的融合,这对舞蹈钢琴伴奏者提出了较高的专业要求.

  9. The possibility of life proliferation from Enceladus

    Czechowski, Leszek

    2016-07-01

    Enceladus is a medium sized icy satellite (MIS) of Saturn. MIS are built of mixtures of rocks and ices. According to [1]: "For life to have emerged […] on the early Earth, a sustained source of chemically transducible energy was essential. The serpentinization process is emerging as an increasingly likely source of that energy" (see also [2]). We consider here conditions for origin of life in the early Enceladus and later proliferation of the life. Mass of serpentinite: The serpentinization on the Earth is often considered with hydrothermal activity in neovolcanic zones along mid-oceanic spreading centers. However, only in small part the hydrothermal activity really occurs. A simple calculations (e.g. [3]) indicate that mass fraction of silicates in Enceladus is ~0.646, hence the total mass of its silicate is ~6.97 10^1^9 kg. [4] considered the process of differentiation and core forming in Enceladus. He found that the result of differentiation is a relatively cold core of loosely packed grains with water between them. The entire core of Enceladus was probably permeable. This could lead to formation of extensive hydrothermal convective systems. It indicates that total mass of serpententinized silicate in Enceladus could be larger than on the Earth. The evolution of temperature in the Enceladus interior for the first a few hundreds Myr is given in [4]. He found that the temperature allows for existing the life even in the center of the satellite. It is possible that for hundreds of Myr the conditions in Enceladus were more favorable for origin of life than on the Earth. Proliferation of life: The low gravity of the Enceladus and its volcanic activity make transport possible. Note that the low temperature of plumes from active region of Enceladus does not kill the organisms. The primitive bacteria could leave the Enceladus with volcanic jets in the same way as particles of the E ring. Other mechanisms could transport particles to terrestrial planets. Therefore it

  10. Extended Deterrence, Nuclear Proliferation, and START III

    Speed, R.D.

    2000-06-20

    Early in the Cold War, the United States adopted a policy of ''extended nuclear deterrence'' to protect its allies by threatening a nuclear strike against any state that attacks these allies. This threat can (in principle) be used to try to deter an enemy attack using conventional weapons or one using nuclear, chemical, or biological weapons. The credibility of a nuclear threat has long been subject to debate and is dependent on many complex geopolitical factors, not the least of which is the military capabilities of the opposing sides. The ending of the Cold War has led to a significant decrease in the number of strategic nuclear weapons deployed by the United States and Russia. START II, which was recently ratified by the Russian Duma, will (if implemented) reduce the number deployed strategic nuclear weapons on each side to 3500, compared to a level of over 11,000 at the end of the Cold War in 1991. The tentative limit established by Presidents Clinton and Yeltsin for START III would reduce the strategic force level to 2000-2500. However, the Russians (along with a number of arms control advocates) now argue that the level should be reduced even further--to 1500 warheads or less. The conventional view is that ''deep cuts'' in nuclear weapons are necessary to discourage nuclear proliferation. Thus, as part of the bargain to get the non-nuclear states to agree to the renewal of the Nuclear Non-Proliferation Treaty, the United States pledged to work towards greater reductions in strategic forces. Without movement in the direction of deep cuts, it is thought by many analysts that some countries may decide to build their own nuclear weapons. Indeed, this was part of the rationale India used to justify its own nuclear weapons program. However, there is also some concern that deep cuts (to 1500 or lower) in the U.S. strategic nuclear arsenal could have the opposite effect. The fear is that such cuts might undermine extended

  11. Suppression of WIF-1 through promoter hypermethylation causes accelerated proliferation of the aryl hydrocarbon receptor (AHR) overexpressing MCF10AT1 breast cancer cells

    Highlights: → 5-Aza-2'-deoxycytidine (AZ) causes proliferation suppression and ERα recovery. → AZ down-regulates Wnt/β-catenin pathway mainly by increasing WIF-1 expression. → Both ERα and AhR have some effects on DNA methylation in breast cancer cells. → Artificial overexpression of ERα in ER negative cells increases WIF-1 expression. → WIF-1 promoter hypermethylation is one of the major causes for accelerated proliferation. -- Abstract: The cause for increased cell proliferation in AHR overexpressing breast cancer cells still remains unknown. Here we studied the molecular basis of aggressive cell proliferation of an AHR overexpressing and ERα functionally down-regulated MCF10AT1 cell line, designated as P20E, in comparison to a matched sub-line, P20C with normal AHR expression and ERα function. We found that a 4-day treatment of P20E cells with 5-aza-2'-deoxycytidine (AZ) caused a significant suppression of cell proliferation. Such an effect of AZ was accompanied with the significant recovery of ERα function. Among diagnostic markers of AZ-induced cellular changes we found conspicuous up-regulation of mRNA expression of Wnt inhibitory factor-1 (WIF-1), particularly in P20E. The possibility of AZ-induced demethylation on the promoter of WIF-1 gene was confirmed through methylation specific PCR assay. Such AZ-induced changes in P20E cells were also accompanied with the decrease in the binding of nuclear proteins to the 32P labeled TRE (TCF response element) and the reduced accumulation of β-catenin protein in the cell nucleus, indicating the importance of Wnt/β-catenin pathway in maintaining the increased cell proliferation in P20E line over P20C line. The importance of WIF-1 in this regard has been validated by transfecting cells with siRNA against WIF-1, which caused an increase in cell proliferation. Moreover, artificial overexpression of ERα in both P20E as well as MDA-MB-231 cells increased the mRNA expression of WIF-1. Together these

  12. Inactivating the spindle checkpoint kinase Bub1 during embryonic development results in a global shutdown of proliferation

    Taylor Stephen S

    2009-09-01

    Full Text Available Abstract Background Bub1 is a component of the spindle assembly checkpoint, a surveillance mechanism that maintains chromosome stability during M-phase. Bub1 is essential during the early stages of embryogenesis, with homozygous BUB1-null mice dying shortly after day E3.5. Bub1 is also required later during embryogenesis; inactivation of BUB1 on day E10.5 appears to rapidly block all further development. However, the mechanism(s responsible for this phenotype remain unclear. Findings Here we show that inactivating BUB1 on day E10.5 stalls embryogenesis within 48 hours. This is accompanied by a global shutdown of proliferation, widespread apoptosis and haemorrhaging. Conclusion Our results suggest that Bub1 is required throughout the developing embryo for cellular proliferation. Therefore, Bub1 has been shown to be essential in all scenarios analyzed thus far in mice: proliferation of cultured fibroblasts, spermatogenesis, oogenesis and both early and late embryonic development. This likely reflects the fact that Bub1 has dual functions during mitosis, being required for both SAC function and chromosome alignment.

  13. Clinical application of percutanously interventional therapeusis in patients with hepatocellular carcinoma accompanied with bile duct thrombosis

    Objective: To explore the method and value of percutanously interventional therapeusis for treatment of obstructive jaundice caused by hepatocellular carcinoma accompanied with bile duct thrombosis. Methods: Sixteen cases bile duct thrombosis proved by pathology and imaging examinations were retrospectively analyzed. According to the clinical symptoms, all the patients received percutaneous transhepatic biliary drainage (PTBD) including permanent external drainage, temporary internal drainage and implantation of covered stents. Serum total bilirubin (TBIL) after the interventional therapeusis were measured and compared with that before the treatments by t test to evaluate the efficacy of these treatments. The relief of clinical symptoms was also reviewed to evaluate the efficacy of these treatments. The relief of clinical symptoms was also reviewed to evaluate the efficacy of these treatments. The patients were followed up within 2 years. Results: The PTBD was successfully performed in 16 cases. Permanent external drainage, temporary internal drainage and imalantation of covered stents were performed in 2 patients, 7 patients and 7 patients respectively. TBIL after the interventional therapy decreased significantly (t=7.366, P<0.01) to (161.2 ± 80.5) μmol/L averagely from (261.9 ± 77.2) μmol/L before the treatments. All the patients died before the end of followed-up. The average survival time was 204 days (30 to 391 d) and the median survival time was 200 days. Bleeding and infection were the main complications, which could be controlled successfully by routine treatments. Conclusion: With high achievement ratio and good efficacy, percutanously interventional therapeusis are good choices for the treatments of obstructive jaundice due to bile duct thrombosis. (authors)

  14. Conceptual approach to 'eternal' accompaniment of decommissioning: human factor and public relations

    It is necessary at once to define that 'eternity' of accompaniment is not bound with exploitation of the future power engineering (hardly predicted and unnecessary priority nuclear). It will be the long-time (on hundreds years) control for the outcomes and consequences of decommissioning of a current nuclear power engineering infrastructure. Public relations at nuclear power plant (NPP) decommissioning are indispensable component at all stages of such process, so else long before it's beginning. The NPP 'exploiting' society with necessity should realize high manufacturability of branch and conforming large volumes of the material and intellectual investments at all stages of productive exploitation within several decades and not smaller costs for decommissioning during of ... several centuries. Moreover, it is not alternative to decommissioning at any versions 'green' prohibition or, to the contrary, nuclear lobbying of adequacy of nuclear power engineering. This expense nonalternative should be explained, realized, and perceived by society for regulation, control and implementation by several generations of the performers. Certainly, this problem is extremely complex, and first of all, psychologically for the nuclear professionals. Unfortunately, it is necessary to state the dangerous tendency of a creation postponement of design algorithm for the long-time public relations on this problem. And it is a tragic Chernobyl lesson already.... It is persistently demonstrated in twenty years impossibility of repetition of April 26 after the count and correcting of design errors, steam-gaseous effect and graphite-phase transition. But, do not give My God.., people in cities near NPP (really worldwide?) not at once will shut window leaves, not everyone will drink iodine solution at the first hours and will dress on headgears etc. But so has received that after 'instantaneous' break of the ChNPP unit 4 the decommissioning experience has appeared. There is no requiring of

  15. One-way-coupling simulation of cavitation accompanied by high-speed droplet impact

    Kondo, Tomoki; Ando, Keita

    2016-03-01

    Erosion due to high-speed droplet impact is a crucial issue in industrial applications. The erosion is caused by the water-hammer loading on material surfaces and possibly by the reloading from collapsing cavitation bubbles that appear within the droplet. Here, we simulate the dynamics of cavitation bubbles accompanied by high-speed droplet impact against a deformable wall in order to see whether the bubble collapse is violent enough to give rise to cavitation erosion on the wall. The evolution of pressure waves in a single water (or gelatin) droplet to collide with a deformable wall at speed up to 110 m/s is inferred from simulations of multicomponent Euler flow where phase changes are not permitted. Then, we examine the dynamics of cavitation bubbles nucleated from micron/submicron-sized gas bubble nuclei that are supposed to exist inside the droplet. For simplicity, we perform Rayleigh-Plesset-type calculations in a one-way-coupling manner, namely, the bubble dynamics are determined according to the pressure variation obtained from the Euler flow simulation. In the simulation, the preexisting bubble nuclei whose size is either micron or submicron show large growth to submillimeters because tension inside the droplet is obtained through interaction of the pressure waves and the droplet interface; this supports the possibility of having cavitation due to the droplet impact. It is also found, in particular, for the case of cavitation arising from very small nuclei such as nanobubbles, that radiated pressure from the cavitation bubble collapse can overwhelm the water-hammer pressure directly created by the impact. Hence, cavitation may need to be accounted for when it comes to discussing erosion in the droplet impact problem.

  16. Increased expression of bcl11b leads to chemoresistance accompanied by G1 accumulation.

    Piotr Grabarczyk

    Full Text Available BACKGROUND: The expression of BCL11B was reported in T-cells, neurons and keratinocytes. Aberrations of BCL11B locus leading to abnormal gene transcription were identified in human hematological disorders and corresponding animal models. Recently, the elevated levels of Bcl11b protein have been described in a subset of squameous cell carcinoma cases. Despite the rapidly accumulating knowledge concerning Bcl11b biology, the contribution of this protein to normal or transformed cell homeostasis remains open. METHODOLOGY/PRINCIPAL FINDINGS: Here, by employing an overexpression strategy we revealed formerly unidentified features of Bcl11b. Two different T-cell lines were forced to express BCL11B at levels similar to those observed in primary T-cell leukemias. This resulted in markedly increased resistance to radiomimetic drugs while no influence on death-receptor apoptotic pathway was observed. Apoptosis resistance triggered by BCL11B overexpression was accompanied by a cell cycle delay caused by accumulation of cells at G1. This cell cycle restriction was associated with upregulation of CDKN1C (p57 and CDKN2C (p18 cyclin dependent kinase inhibitors. Moreover, p27 and p130 proteins accumulated and the SKP2 gene encoding a protein of the ubiquitin-binding complex responsible for their degradation was repressed. Furthermore, the expression of the MYCN oncogene was silenced which resulted in significant depletion of the protein in cells expressing high BCL11B levels. Both cell cycle restriction and resistance to DNA-damage-induced apoptosis coincided and required the histone deacetylase binding N-terminal domain of Bcl11b. The sensitivity to genotoxic stress could be restored by the histone deacetylase inhibitor trichostatine A. CONCLUSIONS: The data presented here suggest a potential role of BCL11B in tumor survival and encourage developing Bcl11b-inhibitory approaches as a potential tool to specifically target chemoresistant tumor cells.

  17. Theory of the jitter radiation in a magnetized plasma accompanying a temperature gradient

    Hattori, Makoto; Fujiki, Kazushiro

    2016-04-01

    The linear stability of a magnetized plasma accompanying a temperature gradient is reexamined by using plasma kinetic theory. We propose that the anisotropic velocity distribution function should be decomposed into two components. One is proportional to the temperature gradient parallel to the background magnetic field. The other is proportional to the temperature gradient perpendicular to the background magnetic field. Since the amplitude of the anisotropic velocity distribution function is proportional to the heat conductivity, and the heat conductivity perpendicular to the magnetic field is strongly reduced, the first component of the anisotropic velocity distribution function is predominant. The anisotropic velocity distribution function induced by the temperature gradient along the background magnetic field drives plasma kinetic instability and circular polarized magnetic plasma waves are excited. We show that the instability is almost identical to the Weibel instability in the weakly magnetized plasma. However, in the case of the instability caused by the temperature gradient, whether wave vectors of modes are parallel to or antiparallel to the background magnetic field, the growth rate of one mode is suppressed and the growth rate of the other mode is enhanced due to the background magnetic field. In the strongly magnetized plasma, one mode is stabilized and only one of the modes remains unstable. The formulae for the jitter radiation spectrum emitted by relativistic electrons when they travel through the magnetized plasma with the plasma waves driven by the instability are deduced at the first time. We show that the synchrotron emission and the jitter radiation are simultaneously emitted from the same relativistic electron. The jitter radiation is expected to be circularly polarized but with a very small polarization degree since almost the same amounts of left-handed and right-handed circular polarized magnetic waves are excited by the instability.

  18. Case of Severe Maxillary Protrusion Accompanied by Crowding and Scissor Bite.

    Katada, Hidenori; Sueishi, Kenji

    2015-01-01

    This case involved a 30-year-old woman who visited our hospital with the main complaint of protrusion of the maxillary incisors and upper and lower lips. She had difficulty closing her lips, and a chin button was observed when the lips were closed. The skeletal pattern showed maxillary protrusion and mandibular retrusion, and the mandible showed severe high angle. Labial inclination of both the maxillary and mandibular incisors was found, as well as crowding. In addition, the maxillary left second molar showed buccal displacement, and scissor bite was evident in the left second molar region. The bilateral molar relationship was cusp-to-cusp class II malocclusion. Angle class II maxillary protrusion accompanied by crowding and left second molar scissor bite was diagnosed. Surgical orthodontic treatment was judged as the best approach to treat the jaw deformities. However, in line with the wishes of the patient, treatment was undertaken using implant anchors instead. Straight-wire brackets with a 0.022-inch slot were fitted. A lingual arch was placed in the mandible and plate-type implant anchors in the first molar region of the maxilla. Almost no change was observed in skeletal pattern as no surgery was performed. The maxillary incisors moved back 10 mm, however, and the mandibular incisors showed an improvement of 4 mm from L1 to APo. The upper and lower lips consequently moved back 7 mm with respect to the E-line. Active treatment required 3 years and 6 months. Esthetic and functional improvements were achieved. PMID:26657523

  19. Magnetic Resonance Imaging of Mitochondrial Dysfunction and Metabolic Activity, Accompanied by Overproduction of Superoxide.

    Bakalova, Rumiana; Georgieva, Ekaterina; Ivanova, Donika; Zhelev, Zhivko; Aoki, Ichio; Saga, Tsuneo

    2015-12-16

    This study shows that a mitochondria-penetrating nitroxide probe (mito-TEMPO) allows detection of superoxide and visualization of mitochondrial dysfunction in living cells due to the effect of T1 shortening in MRI. Mitochondrial dysfunction was induced by treatment of cells with rotenone and 2-methoxyestradiol (2-ME/Rot). The MRI measurements were performed on 7T MRI. The 2-ME/Rot-treated cells were characterized by overproduction of superoxide, which was confirmed by a conventional dihydroethidium test. In the presence of mito-TEMPO, the intensity of MRI signal in 2-ME/Rot-treated cells was ∼30-40% higher, in comparison with that in untreated cells or culture media. In model (cell-free) systems, we observed that superoxide, but not hydrogen peroxide, increased the intensity of T1-weighted MRI signal of mito-TEMPO. Moreover, the superoxide restores the T1-weighted MRI contrast of mito-TEMPOH, a noncontrast (diamagnetic) analogue of mito-TEMPO. This was also confirmed by using EPR spectroscopy. The results demonstrate that superoxide radical is involved in the enhancement of T1-weighted MRI contrast in living cells, in the absence and presence of mito-TEMPO. This report gives a direction for discovering new opportunities for functional MRI, for detection of metabolic activity, accompanied by overproduction of superoxide, as well as by disturbance of the balance between superoxide and hydrogen peroxide, a very important approach to clarify the fine molecular mechanisms in the regulation of many pathologies. The visualization of mitochondrial activity in real-time can be crucial to clarify the molecular mechanism of the functional MRI in its commonly accepted definition, as a method for detection of neurovascular coupling. PMID:26367059

  20. A Study of Using Massage Therapy Accompanied with Stretching Exercise for Rehabilitation of Mammary Gland Hyperplasia.

    Lv, Pin; Chong, Yuping; Zou, Huagang; Chen, Xiangxian

    2016-01-01

    Purpose. To apply massage therapy accompanied with stretching exercises for treatment of mammary gland hyperplasia, evaluate the clinical outcome in patients, and estimate the therapy as a novel treatment method for mammary hyperplasia. Methods. 28 adult female patients were selected and treated with massage therapy and stretching exercises focusing on skeleton muscles of chest, abdomen, and axilla. The mammary gland oxyhemoglobin (OxyHb) and deoxyhemoglobin (DeoxyHb) levels were detected before and after treatment after 15, 30, and 45 days. Results. In this cohort, pretreatment OxyHb (mean ± SD) is 1.32 ± 0.14 (medium-high), and DeoxyHb is 0.87 ± 0.13 (normal). All patients were clinically diagnosed with benign mammary gland hyperplasia and mastitis. The posttreatment OxyHb levels are 1.23 ± 0.09 (normal-medium, 15-day), 1.16 ± 0.08 (normal, 30-day), and 1.05 ± 0.04 (normal, 45-day), and DeoxyHb levels are 0.90 ± 0.11 (normal, 15-day), 0.94 ± 0.18 (normal, 30-day), and 0.98 ± 0.12 (normal, 45-day). Patients were diagnosed with decreased hyperplasia 15 and 30 days after treatment and with no symptom of hyperplasia in mammary gland 45 days after treatment. Conclusion. Mammary gland hyperplasia is closely correlated with pathological changes of skeletal muscles and could be significantly improved by massage therapy and stretching exercises targeting neighboring skeletal muscles. PMID:27022615

  1. Processes accompanying of mantle plume emplacement into continental lithosphere: Evidence from NW Arabian plate, Western Syria

    Sharkov, E. V.

    2015-12-01

    Lower crustal xenoliths occurred in the Middle Cretaceous lamprophyre diatremes in Jabel Ansaria (Western Syria) (Sharkov et al., 1992). They are represented mainly garnet granulites and eclogite-like rocks, which underwent by deformations and retrograde metamorphism, and younger fresh pegmatoid garnet-kaersutite-clinopyroxene (Al-Ti augite) rocks; mantle peridotites are absent in these populations. According to mineralogical geothermobarometers, forming of garnet-granulite suite rocks occurred under pressure 13.5-15.4 kbar (depths 45-54 kn) and temperature 965-1115oC. At the same time, among populations of mantle xenoliths in the Late Cenozoic platobasalts of the region, quite the contrary, lower crustal xenoliths are absent, however, predominated spinel lherzolites (fragments of upper cooled rim of a plume head), derived from the close depths (30-40 km: Sharkov, Bogatikov, 2015). From this follows that ancient continental crust was existed here even in the Middle Cretaceous, but in the Late Cenozoic was removed by extended mantle plume head; at that upper sialic crust was not involved in geomechanic processes, because Precambrian metamorphic rocks survived as a basement for Cambrian to Cenozoic sedimentary cover of Arabian platform. In other words, though cardinal rebuilding of deep-seated structure of the region occurred in the Late Cenozoic but it did not affect on the upper shell of the ancient lithosphere. Because composition of mantle xenolithis in basalts is practically similar worldwide, we suggest that deep-seated processes are analogous also. As emplacement of the mantle plume heads accompanied by powerful basaltic magmatism, very likely that range of lower (mafic) continental crust existence is very convenient for extension of plume heads and their adiabatic melting. If such level, because of whatever reasons, was not reached, melting was limited but appeared excess of volatile matters which led to forming of lamprophyre or even kimberlite.

  2. Sonic hedgehog (Shh/Gli modulates the spatial organization of neuroepithelial cell proliferation in the developing chick optic tectum

    Rapacioli Melina

    2012-10-01

    Full Text Available Abstract Background Sonic hedgehog (Shh/Gli pathway plays an important regulatory role on the neuroepithelial cells (NEc proliferation in the dorsal regions of the developing vertebrate Central Nervous System. The aim of this paper was to analyze the effect of the Shh/Gli signaling pathway activation on the proliferation dynamics and/or the spatial organization of the NEc proliferation activity during early stages of the developing chick optic tectum (OT. In ovo pharmacological gain and loss of hedgehog function approaches were complemented with in vivo electroporation experiments in order to create ectopic sources of either Shh or Gli activator (GliA proteins in the OT. NEc proliferating activity was analyzed at ED 4/4.5 by recording the spatial co-ordinates of the entire population of mitotic NEc (mNEc located along OT dorsal-ventral sections. Several space signals (numerical sequences were derived from the mNEc spatial co-ordinate records and analyzed by different standardized non-linear methods of signal analysis. Results In ovo pharmacologic treatment with cyclopamine resulted in dramatic failure in the OT expansion while the agonist purmorphamine produced the opposite result, a huge expansion of the OT vesicle. Besides, GliA and Shh misexpressions interfere with the formation of the intertectal fissure located along the dorsal midline. This morphogenetic alteration is accompanied by an increase in the mNEc density. There is a gradient in the response of NEcs to Shh and GliA: the increase in mNEc density is maximal near the dorsal regions and decrease towards the OT-tegmental boundary. Biomathematical analyses of the signals derived from the mNEc records show that both Shh and GliA electroporations change the proliferation dynamics and the spatial organization of the mNEc as revealed by the changes in the scaling index estimated by these methods. Conclusions The present results show that the Shh/Gli signaling pathway plays a critical role

  3. Peptic ulcers accompanied with gastrointestinal bleeding, pylorus obstruction and cholangitis secondary to choledochoduodenal fistula: A case report

    XI, BIN; JIA, JUN-JUN; LIN, BING-YI; GENG, LEI; ZHENG, SHU-SEN

    2016-01-01

    Peptic ulcers are an extremely common condition, usually occurring in the stomach and proximal duodenum. However, cases of peptic ulcers accompanied with multiple complications are extremely rare and hard to treat. The present case reinforces the requirement for the early recognition and correct treatment of peptic ulcers accompanied with multiple complications. A 67-year-old man presented with recurrent abdominal pain, fever and melena. The laboratory results showed anemia (hemoglobin 62 g/l) and hypoproteinemia (23 g/l). Abdominal imaging examinations revealed stones in the gallbladder and right liver, with air in the dilated intrahepatic and extrahepatic bile ducts. Endoscopic retrograde cholangiopancreatography failed due to a deformed pylorus. The patient was finally diagnosed with peptic ulcers accompanied with gastrointestinal (GI) bleeding, pylorus obstruction and cholangitis secondary to a choledochoduodenal fistula during an emergency pancreatoduodenectomy, which was performed due to a massive hemorrhage of the GI tract. The patient recovered well after the surgery. PMID:26870237

  4. A successful laparoscopic neovaginoplasty using peritoneum in Müllerian agenesis with inguinal ovaries accompanied by primary ovarian insufficiency

    Gweon, Seonghye; Lee, Jisun; Hwang, Suna; Hwang, Kyoung Joo

    2016-01-01

    The combination of Müllerian agenesis with inguinal ovaries accompanied by primary ovarian insufficiency is extremely rare. A 21-year-old Korean woman was referred to our center with primary amenorrhea. The patient was diagnosed with Müllerian agenesis with inguinal ovaries. Her hormonal profile showed hypergonadotrophic hypogonadism suggesting primary ovarian insufficiency. We performed laparoscopic neovaginoplasty using modified Davydov's procedure and reposition inguinal ovaries in the pelvic cavity. Oral estrogen replacement was applied for the treatment of primary ovarian insufficiency. This is a rare case report on Mayer-Rokitansky-Kuster-Hauser syndrome accompanied not only by inguinal ovaries but also with primary ovarian insufficiency. We present our first experience on the laparoscopic neovaginoplasty performed on the patient with müllerian agenesis accompanied by inguinal ovaries and primary ovarian insufficiency. PMID:27462606

  5. EGFR signaling regulates the proliferation of Drosophila adult midgut progenitors

    Jiang, Huaqi; Edgar, Bruce A.

    2009-01-01

    In holometabolous insects, the adult appendages and internal organs form anew from larval progenitor cells during metamorphosis. As described here, the adult Drosophila midgut, including intestinal stem cells (ISCs), develops from adult midgut progenitor cells (AMPs) that proliferate during larval development in two phases. Dividing AMPs first disperse, but later proliferate within distinct islands, forming large cell clusters that eventually fuse during metamorphosis ...

  6. Non-proliferation aspects of long term assurance of supply

    The meetings in this section deal with the non-proliferation aspects of long-term assurance of supply of the nuclear fuel cycle. A list of 12 fundamental questions concerning the observation and application of the non-proliferation regulations is followed by the comments made by representatives of 10 countries

  7. Constraining potential nuclear-weapons proliferation from civilian reactors

    Cessation of the Cold War and renewed international attention to the proliferation of weapons of mass destruction are leading to national policies aimed at restraining nuclear-weapons proliferation that could occur through the nuclear-fuel cycle. Argonne, which has unique experience, technology, and capabilities, is one of the US national laboratories contributing to this nonproliferation effort

  8. Twenty years of the Non-proliferation Treaty

    The report assesses the achievements of the Non-proliferation Treaty (NPT) and discusses ways in which the non-proliferation regime could be strenghtened. It recommends a series of measures to be taken by the parties of the Treaty, both nuclear and non-nuclear weapons states, with a view to reinforcing the Treaty and achieving its universality. 198 refs

  9. Non proliferation 1980 - INFCE and TnP

    The TNP (non-proliferation agreement of nuclear weapons), its non proliferation policy and the peaceful use of fuel cycle are described, as well as the safeguards of IAEA. It is also mentioned the functions of INFCE (International Nuclear Fuel Cycle Evaluation). (A.B.T.)

  10. Flaws in the Non-Proliferation Treaty

    The Treaty on the Non-Proliferation of Nucler Weapons has the twin objectives of stopping the further spread of nuclear weapons and ending the nuclear arms race on the one hand, and promoting peaceful uses of atomic energy on the other. In quantitative and symbolic terms the NPT is a huge success. More than two-thirds of the world's nations have signed on, making this the most popular arms control agreement on earth. Not a single nation has declared itself to be a nuclear-weapons state beyond the original five members of the ''nuclear club'' who qualified for weapons status under the terms of the Treaty itself: the United States, the Soviet Union, the United Kingdom, France, and China. No party to the Treaty has exercised the permitted option to drop out, and none has been found by the International Atomic Energy Agency (IAEA) to have diverted nuclear material from civil to weapons purposes. Nor has any party been known to have violated NPT prohibitions on developing or assisting other nations to develop nuclear weapons

  11. Atypical proliferating mucinous tumors of gigantic dimensions

    Likić-Lađević Ivana

    2008-01-01

    Full Text Available Background. Ovarian tumors of low malignant potential (LMP are also known as atypically proliferating tumors. Ovarian tumors of LPM account for approximately 15% of all epithelial ovarian cancers. Mean age of occurrence is 40 years and they are 15-20 cm in diameter. Case report. A 32-year-old female patient was hospitalized as an urgent case with a large tumor mass that filled the entire abdomen. Cyst was 100 × 70 cm dimensions belonging to the right ovary and filled with 18 liters of content. Right adnexectomy, resection of the second ovary, as well as biopsy of the omentum were performed. Lymphadenectomy of the right iliac and obturator area was also performed. After receiving definitive histopathological results it was decided to perform a radical reoperation. On the 10th postoperative day relaparotomy, total hysterectomy and left adnexectomy were performed. The patient was released on the 6th postoperative day. She used to come to regular examinations up to date. Conclusion. This case is a proof that LMP tumors have low malignant potential, they grow slowly and can reach great proportions.

  12. Quantitative analysis of in vivo cell proliferation.

    Cameron, Heather A

    2006-11-01

    Injection and immunohistochemical detection of 5-bromo-2'-deoxyuridine (BrdU) has become the standard method for studying the birth and survival of neurons, glia, and other cell types in the nervous system. BrdU, a thymidine analog, becomes stably incorporated into DNA during the S-phase of mitosis. Because DNA containing BrdU can be specifically recognized by antibodies, this method allows dividing cells to be marked at any given time and then identified at time points from a few minutes to several years later. BrdU immunohistochemistry is suitable for cell counting to examine the regulation of cell proliferation and cell fate. It can be combined with labeling by other antibodies, allowing confocal analysis of cell phenotype or expression of other proteins. The potential for nonspecific labeling and toxicity are discussed. Although BrdU immunohistochemistry has almost completely replaced tritiated thymidine autoradiography for labeling dividing cells, this method and situations in which it is still useful are also described. PMID:18428635

  13. Proliferating cell nuclear antigen in neutrophil fate.

    Witko-Sarsat, Véronique; Ohayon, Delphine

    2016-09-01

    The life span of a neutrophil is a tightly regulated process as extended survival is beneficial for pathogen elimination and cell death necessary to prevent cytotoxic content release from activated neutrophils at the inflammatory site. Therefore, the control between survival and death must be a dynamic process. We have previously described that proliferating cell nuclear antigen (PCNA) which is known as a nuclear protein pivotal in DNA synthesis, is a key element in controlling neutrophil survival through its association with procaspases. Contrary to the dogma which asserted that PCNA has a strictly nuclear function, in mature neutrophils, PCNA is present exclusively within the cytosol due to its nuclear export at the end of the granulocytic differentiation. More recent studies are consistent with the notion that the cytosolic scaffold of PCNA is aimed at modulating neutrophil fate rather than simply preventing death. Ultimately, targeting neutrophil survival might have important applications not just in the field of immunology and inflammation, but also in hematology and transfusion. The neutrophil emerges as a unique and powerful cellular model to unravel the basic mechanisms governing the cell cycle-independent functions of PCNA and should be considered as a leader of the pack. PMID:27558345

  14. Nuclear Naval Propulsion: A Feasible Proliferation Pathway?

    Swift, Alicia L.

    2014-01-31

    There is no better time than now to close the loophole in Article IV of the Nuclear Non-proliferation Treaty (NPT) that excludes military uses of fissile material from nuclear safeguards. Several countries have declared their intention to pursue and develop naval reactor technology, including Argentina, Brazil, Iran, and Pakistan, while other countries such as China, India, Russia, and the United States are expanding their capabilities. With only a minority of countries using low enriched uranium (LEU) fuel in their naval reactors, it is possible that a state could produce highly enriched uranium (HEU) under the guise of a nuclear navy while actually stockpiling the material for a nuclear weapon program. This paper examines the likelihood that non-nuclear weapon states exploit the loophole to break out from the NPT and also the regional ramifications of deterrence and regional stability of expanding naval forces. Possible solutions to close the loophole are discussed, including expanding the scope of the Fissile Material Cut-off Treaty, employing LEU fuel instead of HEU fuel in naval reactors, amending the NPT, creating an export control regime for naval nuclear reactors, and forming individual naval reactor safeguards agreements.

  15. Intelligent hand-portable proliferation sensing system

    Dieckman, S.L.; Bostrom, G.A.; Waterfield, L.G.; Jendrzejczyk, J.A.; Ahuja, S.; Raptis, A.C.

    1997-08-01

    Argonne National Laboratory, with support from DOE`s Office of Nonproliferation and National Security, is currently developing an intelligent hand-portable sensor system. This system is designed specifically to support the intelligence community with the task of in-field sensing of nuclear proliferation and related activities. Based upon pulsed laser photo-ionization time-of-flight mass spectrometry technology, this novel sensing system is capable of quickly providing a molecular or atomic analysis of specimens. The system is capable of analyzing virtually any gas phase molecule, or molecule that can be induced into the gas phase by (for example) sample heating. This system has the unique advantages of providing unprecedented portability, excellent sensitivity, tremendous fieldability, and a high performance/cost ratio. The system will be capable of operating in a highly automated manner for on-site inspections, and easily modified for other applications such as perimeter monitoring aboard a plane or drone. The paper describes the sensing system.

  16. Satellite cell proliferation in adult skeletal muscle

    Booth, Frank W. (Inventor); Thomason, Donald B. (Inventor); Morrison, Paul R. (Inventor); Stancel, George M. (Inventor)

    1995-01-01

    Novel methods of retroviral-mediated gene transfer for the in vivo corporation and stable expression of eukaryotic or prokaryotic foreign genes in tissues of living animals is described. More specifically, methods of incorporating foreign genes into mitotically active cells are disclosed. The constitutive and stable expression of E. coli .beta.-galactosidase gene under the promoter control of the Moloney murine leukemia virus long terminal repeat is employed as a particularly preferred embodiment, by way of example, establishes the model upon which the incorporation of a foreign gene into a mitotically-active living eukaryotic tissue is based. Use of the described methods in therapeutic treatments for genetic diseases, such as those muscular degenerative diseases, is also presented. In muscle tissue, the described processes result in genetically-altered satellite cells which proliferate daughter myoblasts which preferentially fuse to form a single undamaged muscle fiber replacing damaged muscle tissue in a treated animal. The retroviral vector, by way of example, includes a dystrophin gene construct for use in treating muscular dystrophy. The present invention also comprises an experimental model utilizable in the study of the physiological regulation of skeletal muscle gene expression in intact animals.

  17. Canada and international safeguards. Verifying nuclear non-proliferation

    The Non-Proliferation Treaty (NPT) came into force in 1970 and now has about 140 signatory nations. By creating legal barriers against proliferation and by promoting an international non-proliferation ethic, the NPT has promoted international peace and security. A key ingredient has been the confidence generated through verification by IAEA safeguards. By the end of 1988 IAEA safeguards agreements had been concluded with about 100 countries, including Canada. Over 500 nuclear facilities worldwide are under safeguards or contain safeguarded nuclear material. The existence of this credible and effective safeguards system makes international trade in nuclear equipment and materials possible, monitoring the transfer of nuclear technology to developing countries as well as between industrial countries. Canada is committed to non-proliferation and IAEA safeguards. Canadian non-proliferation policy is among the strictest in the world, even though opportunities have been lost to sell Canadian technology abroad as a result

  18. Differential Glutamate Metabolism in Proliferating and Quiescent Mammary Epithelial Cells.

    Coloff, Jonathan L; Murphy, J Patrick; Braun, Craig R; Harris, Isaac S; Shelton, Laura M; Kami, Kenjiro; Gygi, Steven P; Selfors, Laura M; Brugge, Joan S

    2016-05-10

    Mammary epithelial cells transition between periods of proliferation and quiescence during development, menstrual cycles, and pregnancy, and as a result of oncogenic transformation. Utilizing an organotypic 3D tissue culture model coupled with quantitative metabolomics and proteomics, we identified significant differences in glutamate utilization between proliferating and quiescent cells. Relative to quiescent cells, proliferating cells catabolized more glutamate via transaminases to couple non-essential amino acid (NEAA) synthesis to α-ketoglutarate generation and tricarboxylic acid (TCA) cycle anaplerosis. As cells transitioned to quiescence, glutamine consumption and transaminase expression were reduced, while glutamate dehydrogenase (GLUD) was induced, leading to decreased NEAA synthesis. Highly proliferative human tumors display high transaminase and low GLUD expression, suggesting that proliferating cancer cells couple glutamine consumption to NEAA synthesis to promote biosynthesis. These findings describe a competitive and partially redundant relationship between transaminases and GLUD, and they reveal how coupling of glutamate-derived carbon and nitrogen metabolism can be regulated to support cell proliferation. PMID:27133130

  19. Crisis and chance of non-proliferation policy - introduction

    In 1995 the parties of the Non-Proliferation Treaty will have to decide on the extension period of the treaty. Preliminary decisions on the fate of the treaty will be made in 1990. Today the non-proliferation regime is facing the dilemma of a number of inherently ambivalent options: The physical knowledge of all relevant nuclear processes has been proliferated, and knowledge cannot be called back. Non-proliferation is based on control and denial, but both are reflected as discriminatory. From an ecological point of view there can be argued for, as well as against the spread of further nuclear energy facilities. Promotion of export and control of export cannot be reconciled. The non-proliferation regime will not be given a new chance without additional confidence-building measures between South and North and without a comprehensive programme to alleviate the global economic, social and ecological crises. (orig.)

  20. Senegenin promotes in vitro proliferation of human neural progenitor cells

    Fang Shi; Zhigang Liang; Zixuan Guo; Ran Li; Fen Yu; Zhanjun Zhang; Xuan Wang; Xiaomin Wang

    2011-01-01

    Senegenin, an effective component of Polygala tenuifolia root extract, promotes proliferation and differentiation of neural progenitor cells in the hippocampus.However, the effects of senegenin on mesencephalon-derived neural progenitor cells remain poorly understood.Cells from a ventral mesencephalon neural progenitor cell line (ReNcell VM) were utilized as models for pharmaceutical screening.The effects of various senegenin concentrations on cell proliferation were analyzed,demonstrating that high senegenin concentrations (5, 10, 50, and 100 pmo/L), particularly 50 pmol/L, significantly promoted proliferation of ReNcell VM cells.In the mitogen-activated protein kinase signal transduction pathway, senegenin significantly increased phosphorylation levels of extracellular signal-regulated kinases.Moreover, cell proliferation was suppressed by extracellular signal-regulated kinase inhibitors.Results suggested that senegenin contributed to in vitro proliferation of human neural progenitor cells by upregulating phosphorylation of extracellular signal-regulated kinase.

  1. The future of nuclear non-proliferation in South Asia

    Nuclear proliferation in South Asia is currently one of the hot topics in world politics. The concern of the international community, and especially the USA, over this issue is coupled with the fear of nuclear conflict between India and Pakistan. As a result, Washington has been using its 'stick and carrot' policy to persuade the two countries involved not to develop their nuclear programs for military purposes. However both countries have not only continued to develop their nuclear ambitions, but seem to have achieved vertical nuclear proliferation. This paper examines the future non-proliferation in the South Asian region in the 1990s. This will be achieved by looking at the following: the development of the nuclear capabilities of both India and Pakistan; how these programs have been developed; the reasons for acquiring the capability for non-conventional defence; the real fear in terms of nuclear proliferation in the region; the possible options for dealing with nuclear proliferation in South Asia

  2. Deformation effects in the alpha accompanied cold ternary fission of even-even $^{244-260}$Cf isotopes

    Santhosh, K P; Krishnan, Sreejith

    2016-01-01

    Within the Unified ternary fission model (UTFM), the alpha accompanied ternary fission of even-even $^{244-260}$Cf isotopes has been studied by taking the interacting barrier as the sum of Coulomb and proximity potential. For the alpha accompanied ternary fission of 244^Cf isotope, the highest yield is obtained for the fragment combination 108^Ru+4^He+132^Te, which contain near doubly magic nuclei 132^Te (N=80, Z=52). In the case of 246^Cf and 248^Cf isotopes, the highest yield is obtained fo...

  3. Accompanying Problems in Children with Cerebral Palsy and their Relationship to type and Level of Motor Disability

    Amirsalari S; Karimloo M; Khayatzadeh Mahany M

    2011-01-01

    Background and Objective: There are some accompanying problems in children with cerebral palsy. These problems may affect the children and their family even more than cerebral palsy. The aim of this study was to investigate the rate of six common accompanying problems and their relationship to type and level of motor function.Subjects and Methods: In this study, 120 children aged 2 to 14 years old were selected from 4 rehabilitation centers in Tehran. At first consent of the child’s mothers ...

  4. Long-term vision and non-vision dominant behavioral deficits in the 2-VO rats are accompanied by time and regional glial activation in the white matter.

    Xue Song Tian

    Full Text Available The permanent occlusion of common carotid arteries (2-VO in rats has been shown to induce progressive and long-lasting deficits in cognitive performance, however, whether these aberrant behaviors are attributed to visual dysfunction or cognitive impairment and what are the underlying mechanisms, remain controversial. In the present study, vision dominant (Morris water maze and non-vision dominant (voice-cued fear conditioning behavioral tests were assigned to comprehensively evaluate the influence of 2-VO lesion on cognitive behaviors. In the Morris water maze test, escape latencies of 2-VO rats were markedly increased in both hidden and unfixed visible platform tasks, which were accompanied by severe retinal damage. In the voice-cued fear conditioning test, significant reduction in the percentage of freezing behavior was observed at 60 days after 2-VO lesion. Chronic lesion by 2-VO failed to cause noticeable changes in the grey matter, as indicated by intact hippocampal and prefrontal cortical structures, sustained synaptic protein levels and glial cell numbers. In contrast, aberrant arrangement of myelinated axons was observed in the optic tract, but not in the corpus callosum and inner capsule of 2-VO rats. Concurrently, marked astrocyte proliferation and microglia activation in the optic tract occurred at 3 days after 2-VO lesion, and continued for up to 60 days. Differently, robust glial activation was observed in the corpus callosum at 3 days after 2-VO surgery, and then gradually returned to the baseline level at 14 and 60 days. Our study reported for the first time about the effect of 2-VO on the long-term cognitive impairment in the non-vision dominant fear conditioning test, which may be more applicable than the Morris water maze test for assessing 2-VO associated cognitive function. The time and region specific glial activation in the white matter may relate to retinal impairment, even behavioral deficits, in the setting of chronic

  5. Reactivation from latency displays HIV particle budding at plasma membrane, accompanying CD44 upregulation and recruitment

    Sano Kouichi

    2009-07-01

    Full Text Available Abstract Background It has been accepted that HIV buds from the cell surface in T lymphocytes, whereas in macrophages it buds into intracellular endosomes. Recent studies, on the other hand, suggest that HIV preferentially buds from the cell surface even in monocytic cells. However, most studies are based on observations in acutely infected cells and little is known about HIV budding concomitant with reactivation from latency. Such studies would provide a better understanding of a reservoir for HIV. Results We observed HIV budding in latently infected T lymphocytic and monocytic cell lines following TNF-α stimulation and examined the upregulation of host factors that may be involved in particle production. Electron microscopy analysis revealed that reactivation of latently infected J1.1 cells (latently infected Jurkat cells with HIV-1 and U1 cells (latently infected U937 cells with HIV-1 displayed HIV particle budding predominantly at the plasma membrane, a morphology that is similar to particle budding in acutely infected Jurkat and U937 cells. When mRNA expression levels were quantified by qRT-PCR, we found that particle production from reactivated J1.1 and U1 cells was accompanied by CD44 upregulation. This upregulation was similarly observed when Jurkat and U937 cells were acutely infected with HIV-1 but not when just stimulated with TNF-α, suggesting that CD44 upregulation was linked with HIV production but not with cell stimulation. The molecules in endocytic pathways such as CD63 and HRS were also upregulated when U1 cells were reactivated and U937 cells were acutely infected with HIV-1. Confocal microscopy revealed that these upregulated host molecules were recruited to and accumulated at the sites where mature particles were formed at the plasma membrane. Conclusion Our study indicates that HIV particles are budded at the plasma membrane upon reactivation from latency, a morphology that is similar to particle budding in acute

  6. Rhenium and osmium isotope and elemental behaviour accompanying laterite formation in the Deccan region of India

    Wimpenny, J.; Gannoun, A.; Burton, K. W.; Widdowson, M.; James, R. H.; Gíslason, S. R.

    2007-09-01

    This study presents major-, trace-element and Re-Os isotope data for two laterite profiles from the Deccan region in India. The first profile from Bidar developed upon Deccan basalt itself, the second from Goa formed on an Archean greywacke. Both profiles show the typical changes in mineralogy and chemistry accompanying laterite formation; from little altered parent rock at depth, to altered saprolite (depleted in mobile elements), to a highly altered, Si depleted, Fe-rich laterite capped by an iron-rich crust at the surface. Rhenium and Os concentrations show a systematic enrichment with increasing degree of alteration, where the highest concentrations are found in the Fe-rich laterite crust. The level of Os enrichment is greater than that of Re, and as a consequence 187Re/ 188Os ratios are lower in the laterite than the parent rock. With time this leads to the development of 187Os/ 188Os ratios that are also less radiogenic than the parent rock, and this relationship holds irrespective of rock type or age or the time of laterite formation. The marked enrichment of both Re and Os, and the patterns of change above and below the paleowatertable, indicates that both elements are highly mobile. High Eh and low pH conditions facilitate Re and Os dissolution, and redistribution occurs via groundwaters, whereas with increasing pH (higher in the profile) both elements are scavenged out of solution and precipitate either as PGE-rich alloys or are strongly partitioned into oxides or oxyhydroxides. Nevertheless, the isotope and elemental data for both profiles suggests that at least some of the Re and Os must be externally derived, either from aeolian deposition or from the lateral movement of solute-laden groundwaters. Given the high levels of Os in laterites, their extensive geographical coverage of the continents, and their active role in the surface water cycle, it seems likely that they will exert a significant influence on the continental flux of Os to the oceans, that

  7. Pancreatic cancer accompanied by a moderate-sized pseudocyst with extrapancreatic growth

    Pancreatic cancer accompanied by a moderate-sized pseudocyst with extrapancreatic growth is extremely rare. Diagnosis of pancreatic cancer on preoperative imaging is difficult when the pancreatic parenchyma is compressed by a pseudocyst and becomes unclear. Despite advances in imaging techniques, accurate preoperative diagnosis of cystic lesions of the pancreas remains difficult. In this case, it was challenging to diagnose pancreatic cancer preoperatively as we could not accurately assess the pancreatic parenchyma, which had been compressed by a moderate-sized cystic lesion with extrapancreatic growth. A 63-year-old woman underwent investigations for epigastric abdominal pain. She had no history of pancreatitis. Although we suspected pancreatic ductal carcinoma with a pancreatic cyst, there was no mass lesion or low-density area suggestive of pancreatic cancer. We did not immediately suspect pancreatic cancer, as development of a moderate-sized cyst with extrapancreatic growth is extremely rare and known tumor markers were not elevated. Therefore, we initially suspected that a massive benign cyst (mucinous cyst neoplasm, serous cyst neoplasm, or intraductal papillary mucinous neoplasm) resulted in stenosis of the main pancreatic duct. We were unable to reach a definitive diagnosis prior to the operation. We had planned a pancreaticoduodenectomy to reach a definitive diagnosis. However, we could not remove the tumor because of significant invasion of the surrounding tissue (portal vein, superior mesenteric vein, etc.). The fluid content of the cyst was serous, and aspiration cytology from the pancreatic cyst was Class III (no malignancy), but the surrounding white connective tissue samples were positive for pancreatic adenocarcinoma on pathological examination during surgery. We repeated imaging (CT, MRI, endoscopic ultrasound, etc.) postoperatively, but there were neither mass lesions nor a low-density area suggestive of pancreatic cancer. In retrospect, we think

  8. Stereo-Specific Hydrogen Exchange Accompanying Catalytic Hydrogenation of Methyl β, β-Dimethylacrylate

    substrate; the methyl of the ester function does not exchange, (d) The double bond saturation process is not accompanied by intramolecular exchange. (author)

  9. Uterine gland development begins postnatally and is accompanied by estrogen and progesterone receptor expression in the dog.

    Cooke, P S; Borsdorf, D C; Ekman, G C; Doty, K F; Clark, S G; Dziuk, P J; Bartol, F F

    2012-11-01

    During neonatal and juvenile life, mammalian uteri undergo extensive structural and functional changes, including uterine gland differentiation and development. In sheep and mice, inhibition of neonatal uterine gland development induced by progestin treatment led to a permanent aglandular uterine phenotype and adult infertility, suggesting that this strategy might be useful for sterilizing dogs and other companion animals. The goal of this study was to define temporal patterns of adenogenesis (gland development), cell proliferation, and progesterone and estrogen receptor expression in uteri of neonatal and juvenile dogs as a first step toward determining whether neonatal progestin treatments might be a feasible contraceptive approach in this species. Uteri obtained from puppies at postnatal wk 1, 2, 4, 6, or 8 were evaluated histologically and immunostained for MKI67, a marker of cell proliferation, estrogen receptor-1, and progesterone receptor. Adenogenesis was under way at 1 wk of age, as indicated by the presence of nascent glands beginning to bud from the luminal epithelium, and rapid proliferation of both luminal epithelial and stromal cells. By Week 2, glands were clearly identifiable and proliferation of luminal, glandular, and stromal cells was pronounced. At Week 4, increased numbers of endometrial glands were evident penetrating uterine stroma, even as proliferative activity decreased in all cell compartments as compared with Week 2. Whereas gland development was most advanced at Weeks 6 to 8, luminal, glandular, and stromal proliferation was minimal, indicating that the uterus was nearly mitotically quiescent at this age. Both estrogen receptor-1 and progesterone receptor were expressed consistently in uterine stromal and epithelial cells at all ages examined. In summary, canine uterine adenogenesis was underway by 1 wk of age and prepubertal glandular proliferation was essentially complete by Week 6. These results provided information necessary to

  10. Use of SAR data for proliferation monitoring

    Synthetic Aperture Radar (SAR) is an active and coherent system. SAR images are complex data which contain both amplitude and phase information. The analysis of single SAR data required a very good experience and a good understanding of SAR geometry regarding layover, shadowing, texture and speckle. Image analyst can depicts and describes most of the facilities related to nuclear proliferation and weapons of mass destruction (WMD). The Amplitude Change Detection (ACD) technique consists of a combination of two or three SAR amplitude data acquired with similar orbit and frequency parameters on different dates. That technique provides a very good overview of the changes and particularly regarding vehicles activity and constructions ongoing within the area of interest over the monitoring period. One of the particularities of the SAR systems is to be coherent. The phase of a single image is not exploitable. Thus when two or more SAR data have been acquired with identical orbit and frequency parameters, the phases shift are indicators of changes such as structural changes, terrain subsidence or motion. The Multi-Temporal Coherence (MTC) product merged the two type of information previously detailed: the ACD and coherence analysis. It consists of the combination of two amplitude images and the corresponding coherence computed image. The MTC image may highlights changes between two states of a target which on the ACD analysis appeared unchanged. EUSC uses the difference interferometry techniques in order to estimate volumes that have changed between two acquisition dates. The paper is followed by the slides of the presentation. (A.C.)

  11. The Asperger personality - psychopathology, personality structure, and differential diagnosis of adults with autism spectrum disorder without accompanying intellectual impairment

    Strunz, Sandra

    2015-01-01

    Introduction: Autism Spectrum Disorders (ASD) are classified as pervasive developmental disorders and are characterised by impairments in social interaction and communication and by repetitive stereotyped pattern of behaviour and restricted interests. Individuals with ASD without accompanying intellectual impairment often already reached adulthood before an ASD diagnosis is made. Diagnosing ASD in adults is challenging, especially because of difficulties in differentiating ASD from personalit...

  12. 40 CFR 164.23 - Contents of the statement of issues to accompany notice of intent to hold a hearing.

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Contents of the statement of issues to accompany notice of intent to hold a hearing. 164.23 Section 164.23 Protection of Environment ENVIRONMENTAL... environment, and whether, when used in accordance with widespread and commonly recognized practice, it will...

  13. 30 CFR 250.245 - What hydrogen sulfide (H2S) information must accompany the DPP or DOCD?

    2010-07-01

    ... analysis must be consistent with the EPA's risk management plan methodologies outlined in 40 CFR part 68. ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What hydrogen sulfide (H2S) information must... Documents (docd) § 250.245 What hydrogen sulfide (H2S) information must accompany the DPP or DOCD?...

  14. The Contribution of Conceptual Change Texts Accompanied by Concept Mapping to Eleventh-Grade Students Understanding of Cellular Respiration Concepts

    Al khawaldeh, Salem A.; Al Olaimat, Ali M.

    2010-01-01

    The present study conducted to investigate the contribution of conceptual change texts, accompanied by concept mapping instruction to eleventh-grade students' understanding of cellular respiration concepts, and their retention of this understanding. Cellular respiration concepts test was developed as a result of examination of related literature…

  15. Random phase disturbances and tolerance on amplitude and phase spread in driver of two-beam accelerator with accompanying wave

    One investigates into effect of random phase disturbances on particle dynamics at extraction of UHF power from two-beam accelerator driver with accompanying wave. Paper presents the simulation results of beam dynamics in the driver depending on the value of phase disturbances. One determines tolerances for spread in values of amplitude and phase of wave in driver power extractors

  16. Designing an Accompanying Ecosystem to Foster Entrepreneurship among Agronomic and Forestry Engineering Students. Opinion and Commitment of University Lecturers

    Ortiz-Medina, L.; Fernández-Ahumada, E.; Lara-Vélez, P.; Taguas, E. V.; Gallardo-Cobos, R.; del Campillo, M. C.; Guerrero-Ginel, J. E.

    2016-01-01

    In the Higher School of Agronomic and Forestry Engineering of the University of Cordoba, a collective project conceived as an 'ecosystem to support and accompany entrepreneurs' has been proposed. The approach aims to spread and consolidate the entrepreneurial spirit and to respond to the demands of possible stakeholders involved in the whole…

  17. ACCOMPANY: ACceptable robotiCs COMPanions for AgeiNg Years: multidimensional aspects of human-system interactions

    Amirabdollahian, Farshid; Akker, op den Rieks; Bedaf, Sandra; Bormann, Richard; Draper, Heather; Gelderblom, Gert Jan; Gutierrez Ruiz, Carolina; Hewson, David; Iacono, Iolanda; Koay, Kheng Lee; Krose, Ben; Marti, Patrizia; Prevot-Huille, Helene; Reiser, Ulrich; Saunders, Joe; Sorell, Tom; Dautenhahn, Kerstin

    2013-01-01

    With changes in life expectancy across the world, technologies enhancing well-being of individuals, specifically for older people, are subject to a new stream of research and development. In this paper we present the ACCOMPANY project, a pan-European project which focuses on home companion technolog

  18. Accompany: Acceptable robotiCs COMPanions for AgeiNG Years — Multidimensional aspects of human-system interactions

    F. Amirabdollahian; R. op den Akker; S. Bedaf; R. Bormann; H. Draper; V. Evers; G.J. Gelderblom; C. Gutierrez Ruiz; D. Hewson; N. Hu; I. Iacono; K.L. Koay; B. Kröse; P. Marti; H. Michel; H. Prevot-Huille; U. Reiser; J. Saunders; T. Sorell; K. Dautenhahn

    2013-01-01

    Email Print Request Permissions Save to Project With changes in life expectancy across the world, technologies enhancing well-being of individuals, specifically for older people, are subject to a new stream of research and development. In this paper we present the ACCOMPANY project, a pan-European p

  19. Utility of Social Modeling for Proliferation Assessment - Preliminary Assessment

    Coles, Garill A.; Gastelum, Zoe N.; Brothers, Alan J.; Thompson, Sandra E.

    2009-06-01

    This Preliminary Assessment draft report will present the results of a literature search and preliminary assessment of the body of research, analysis methods, models and data deemed to be relevant to the Utility of Social Modeling for Proliferation Assessment research. This report will provide: 1) a description of the problem space and the kinds of information pertinent to the problem space, 2) a discussion of key relevant or representative literature, 3) a discussion of models and modeling approaches judged to be potentially useful to the research, and 4) the next steps of this research that will be pursued based on this preliminary assessment. This draft report represents a technical deliverable for the NA-22 Simulations, Algorithms, and Modeling (SAM) program. Specifically this draft report is the Task 1 deliverable for project PL09-UtilSocial-PD06, Utility of Social Modeling for Proliferation Assessment. This project investigates non-traditional use of social and cultural information to improve nuclear proliferation assessment, including nonproliferation assessment, proliferation resistance assessments, safeguards assessments and other related studies. These assessments often use and create technical information about the State’s posture towards proliferation, the vulnerability of a nuclear energy system to an undesired event, and the effectiveness of safeguards. This project will find and fuse social and technical information by explicitly considering the role of cultural, social and behavioral factors relevant to proliferation. The aim of this research is to describe and demonstrate if and how social science modeling has utility in proliferation assessment.

  20. Hypoxia promotes adipose-derived stem cell proliferation via VEGF

    Phuc Van Pham

    2016-01-01

    Full Text Available Adipose-derived stem cells (ADSCs are a promising mesenchymal stem cell source with therapeutic applications. Recent studies have shown that ADSCs could be expanded in vitro without phenotype changes. This study aimed to evaluate the effect of hypoxia on ADSC proliferation in vitro and to determine the role of vascular endothelial growth factor (VEGF in ADSC proliferation. ADSCs were selectively cultured from the stromal vascular fraction obtained from adipose tissue in DMEM/F12 medium supplemented with 10% fetal bovine serum and 1% antibiotic-antimycotic. ADSCs were cultured under two conditions: hypoxia (5% O2 and normal oxygen (21% O2. The effects of the oxygen concentration on cell proliferation were examined by cell cycle and doubling time. The expression of VEGF was evaluated by the ELISA assay. The role of VEGF in ADSC proliferation was studied by neutralizing VEGF with anti-VEGF monoclonal antibodies. We found that the ADSC proliferation rate was significantly higher under hypoxia compared with normoxia. In hypoxia, ADSCs also triggered VEGF expression. However, neutralizing VEGF with anti-VEGF monoclonal antibodies significantly reduced the proliferation rate. These results suggest that hypoxia stimulated ADSC proliferation in association with VEGF production. [Biomed Res Ther 2016; 3(1.000: 476-482

  1. Modeling boundary conditions for balanced proliferation in metastatic latency

    Taylor, Donald P; Wells, Jakob Z; Savol, Andrej; Chennubhotla, Chakra; Wells, Alan

    2013-01-01

    Purpose Nearly half of cancer metastases become clinically evident five or more years after primary tumor treatment; thus metastatic cells survived without emerging for extended periods. This dormancy has been explained by at least two countervailing scenarios: cellular quiescence and balanced proliferation; these entail dichotomous mechanistic etiologies. To examine the boundary parameters for balanced proliferation, we performed in silico modeling. Experimental Design To illuminate the balanced proliferation hypothesis, we explored the specific boundary probabilities under which proliferating micrometastases would remain dormant. A two-state Markov chain Monte Carlo model simulated micrometastatic proliferation and death according to stochastic survival probabilities. We varied these probabilities across 100 simulated patients each with 1,000 metastatic deposits and documented whether the micrometastases exceeded one million cells, died out, or remained dormant (survived 1,218 generations). Results The simulations revealed a narrow survival probability window (49.7 – 50.8 percent) that allowed for dormancy across a range of starting cell numbers, and even then for only a small fraction of micrometastases. The majority of micrometastases died out quickly even at survival probabilities that led to rapid emergence of a subset of micrometastases. Within dormant metastases, cell populations depended sensitively on small survival probability increments. Conclusions Metastatic dormancy as explained solely by balanced proliferation is bounded by very tight survival probabilities. Considering the far larger survival variability thought to attend fluxing microenvironments, it is more probable that these micrometastatic nodules undergo at least periods of quiescence rather than exclusively being controlled by balanced proliferation. PMID:23329811

  2. Protective effects of anisodamine on cigarette smoke extract-induced airway smooth muscle cell proliferation and tracheal contractility

    Anisodamine, an antagonist of muscarinic acetylcholine receptors (mAChRs), has been used therapeutically to improve smooth muscle function, including microvascular, intestinal and airway spasms. Our previous studies have revealed that airway hyper-reactivity could be prevented by anisodamine. However, whether anisodamine prevents smoking-induced airway smooth muscle (ASM) cell proliferation remained unclear. In this study, a primary culture of rat ASM cells was used to evaluate an ASM phenotype through the ability of the cells to proliferate and express contractile proteins in response to cigarette smoke extract (CSE) and intervention of anisodamine. Our results showed that CSE resulted in an increase in cyclin D1 expression concomitant with the G0/G1-to-S phase transition, and high expression of M2 and M3. Functional studies showed that tracheal hyper-contractility accompanied contractile marker α-SMA high-expression. These changes, which occur only after CSE stimulation, were prevented and reversed by anisodamine, and CSE-induced cyclin D1 expression was significantly inhibited by anisodamine and the specific inhibitor U0126, BAY11-7082 and LY294002. Thus, we concluded that the protective and reversal effects and mechanism of anisodamine on CSE-induced events might involve, at least partially, the ERK, Akt and NF-κB signaling pathways associated with cyclin D1 via mAChRs. Our study validated that anisodamine intervention on ASM cells may contribute to anti-remodeling properties other than bronchodilation. -- Highlights: ► CSE induces tracheal cell proliferation, hyper-contractility and α-SMA expression. ► Anisodamine reverses CSE-induced tracheal hyper-contractility and cell proliferation. ► ERK, PI3K, and NF-κB pathways and cyclin D1 contribute to the reversal effect.

  3. CDK2 differentially controls normal cell senescence and cancer cell proliferation upon exposure to reactive oxygen species

    Hwang, Chae Young; Lee, Seung-Min; Park, Sung Sup [Laboratory of Cell Signaling, Aging Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahangno, Yusong, Daejeon 305-806 (Korea, Republic of); Kwon, Ki-Sun, E-mail: kwonks@kribb.re.kr [Laboratory of Cell Signaling, Aging Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahangno, Yusong, Daejeon 305-806 (Korea, Republic of)

    2012-08-17

    Highlights: Black-Right-Pointing-Pointer H{sub 2}O{sub 2} differently adjusted senescence and proliferation in normal and cancer cells. Black-Right-Pointing-Pointer H{sub 2}O{sub 2} exposure transiently decreased PCNA levels in normal cells. Black-Right-Pointing-Pointer H{sub 2}O{sub 2} exposure transiently increased CDK2 activity in cancer cells. Black-Right-Pointing-Pointer p21{sup Cip1} is likely dispensable when H{sub 2}O{sub 2} induces senescence in normal cells. Black-Right-Pointing-Pointer Suggestively, CDK2 and PCNA play critical roles in H{sub 2}O{sub 2}-induced cell fate decision. -- Abstract: Reactive oxygen species modulate cell fate in a context-dependent manner. Sublethal doses of H{sub 2}O{sub 2} decreased the level of proliferating cell nuclear antigen (PCNA) in normal cells (including primary human dermal fibroblasts and IMR-90 cells) without affecting cyclin-dependent kinase 2 (CDK2) activity, leading to cell cycle arrest and subsequent senescence. In contrast, exposure of cancer cells (such as HeLa and MCF7 cells) to H{sub 2}O{sub 2} increased CDK2 activity with no accompanying change in the PCNA level, leading to cell proliferation. A CDK2 inhibitor, CVT-313, prevented H{sub 2}O{sub 2}-induced cancer cell proliferation. These results support the notion that the cyclin/CDK2/p21{sup Cip1}/PCNA complex plays an important role as a regulator of cell fate decisions.

  4. Protective effects of anisodamine on cigarette smoke extract-induced airway smooth muscle cell proliferation and tracheal contractility

    Xu, Guang-Ni; Yang, Kai; Xu, Zu-Peng; Zhu, Liang; Hou, Li-Na; Qi, Hong; Chen, Hong-Zhuan, E-mail: hongzhuan_chen@hotmail.com; Cui, Yong-Yao, E-mail: yongyaocui@yahoo.com.cn

    2012-07-01

    Anisodamine, an antagonist of muscarinic acetylcholine receptors (mAChRs), has been used therapeutically to improve smooth muscle function, including microvascular, intestinal and airway spasms. Our previous studies have revealed that airway hyper-reactivity could be prevented by anisodamine. However, whether anisodamine prevents smoking-induced airway smooth muscle (ASM) cell proliferation remained unclear. In this study, a primary culture of rat ASM cells was used to evaluate an ASM phenotype through the ability of the cells to proliferate and express contractile proteins in response to cigarette smoke extract (CSE) and intervention of anisodamine. Our results showed that CSE resulted in an increase in cyclin D1 expression concomitant with the G0/G1-to-S phase transition, and high expression of M2 and M3. Functional studies showed that tracheal hyper-contractility accompanied contractile marker α-SMA high-expression. These changes, which occur only after CSE stimulation, were prevented and reversed by anisodamine, and CSE-induced cyclin D1 expression was significantly inhibited by anisodamine and the specific inhibitor U0126, BAY11-7082 and LY294002. Thus, we concluded that the protective and reversal effects and mechanism of anisodamine on CSE-induced events might involve, at least partially, the ERK, Akt and NF-κB signaling pathways associated with cyclin D1 via mAChRs. Our study validated that anisodamine intervention on ASM cells may contribute to anti-remodeling properties other than bronchodilation. -- Highlights: ► CSE induces tracheal cell proliferation, hyper-contractility and α-SMA expression. ► Anisodamine reverses CSE-induced tracheal hyper-contractility and cell proliferation. ► ERK, PI3K, and NF-κB pathways and cyclin D1 contribute to the reversal effect.

  5. The non-proliferation paradigm and the restive outlier

    The nuclear non-proliferation paradigm has rarely remained static. Its logic or the underlying principle has however been singular-non-proliferation should lead to nuclear disarmament, and eventually total elimination. It is the approach to the paradigm that has evolved over the years, often accentuated by, and many a time succumbing to, the transformations in the global security environment. Milestones in this evolution have often been construed as shifts in the paradigm, as newer security imperatives necessitated augmentations in existing approaches to proliferation challenges

  6. Scaffold architecture and fibrin gels promote meniscal cell proliferation

    Stability of the knee relies on the meniscus, a complex connective tissue with poor healing ability. Current meniscal tissue engineering is inadequate, as the signals for increasing meniscal cell proliferation have not been established. In this study, collagen scaffold structure, isotropic or aligned, and fibrin gel addition were tested. Metabolic activity was promoted by fibrin addition. Cellular proliferation, however, was significantly increased by both aligned architectures and fibrin addition. None of the constructs impaired collagen type I production or triggered adverse inflammatory responses. It was demonstrated that both fibrin gel addition and optimized scaffold architecture effectively promote meniscal cell proliferation

  7. Proliferation of massive destruction weapons: fantasy or reality?

    This article evaluates the threat of massive destruction weapons (nuclear, chemical, biological) for Europe and recalls the existing safeguards against the different forms of nuclear proliferation: legal (non-proliferation treaty (NPT), comprehensive nuclear test ban treaty (CTBT), fissile material cut off treaty (FMCT) etc..), technical (fabrication of fissile materials, delays). However, all these safeguards can be overcome as proven by the activities of some countries. The situation of proliferation for the other type of massive destruction weapons is presented too. (J.S.)

  8. The Nuclear Non-Proliferation Policy of the Obama Administration

    The objective of this study is to analyze and foresee trends of international nuclear non-proliferation regimes focused on the nuclear non-proliferation policy of the Obama administration, and suggest national policy directions which promote utilization and development of nuclear energy in Korea. For the effective and efficient implementation of the national nuclear use and development program in current international nuclear environment, many efforts should be made: to actively and positively participate in the international nuclear non-proliferation regime; to strengthen nuclear diplomacy in a more systematic manner; and to strengthen the international nuclear cooperation

  9. Effect of pyrimethamine and sulphadoxine on human lymphocyte proliferation

    Bygbjerg, I C; Odum, Niels; Theander, T G

    1986-01-01

    concentrations 10 times higher than serum values obtained in clinical practice inhibited lymphocyte proliferation irreversibly. PYR in concentrations corresponding to clinical practice quickly and irreversibly suppressed the proliferation of PWM-stimulated cells, and more slowly the proliferation of PPD....... Sulphadoxine (SDX), added in vitro, had no effect on the lymphocytes, while SDX plus PYR had the same effect as PYR alone. Oral intake of SDX plus PYR (Fansidar) also blocked the thymidine synthesis of mitogen-stimulated lymphocytes. The possible consequences of the findings for the use of PYR in malaria...

  10. The endogenous cannabinoid anandamide inhibits human breast cancer cell proliferation

    De Petrocellis, Luciano; Melck, Dominique; Palmisano, Antonella; Bisogno, Tiziana; Laezza, Chiara; Bifulco, Maurizio; Di Marzo, Vincenzo

    1998-01-01

    Anandamide was the first brain metabolite shown to act as a ligand of “central” CB1 cannabinoid receptors. Here we report that the endogenous cannabinoid potently and selectively inhibits the proliferation of human breast cancer cells in vitro. Anandamide dose-dependently inhibited the proliferation of MCF-7 and EFM-19 cells with IC50 values between 0.5 and 1.5 μM and 83–92% maximal inhibition at 5–10 μM. The proliferation of several other nonmammary tumoral cell lines was not affected by 10 ...

  11. TAp73 promotes anti-senescence-anabolism not proliferation

    Agostini, Massimiliano; Niklison-Chirou, Maria Victoria; Catani, Maria Valeria; Knight, Richard A.; Melino, Gerry; Rufini, Alessandro

    2014-01-01

    TAp73, a member of the p53 family, has been traditionally considered a tumor suppressor gene, but a recent report has claimed that it can promote cellular proliferation. This assumption is based on biochemical evidence of activation of anabolic metabolism, with enhanced pentose phosphate shunt (PPP) and nucleotide biosynthesis. Here, while we confirm that TAp73 expression enhances anabolism, we also substantiate its role in inhibiting proliferation and promoting cell death. Hence, we would like to propose an alternative interpretation of the accumulating data linking p73 to cellular metabolism: we suggest that TAp73 promotes anabolism to counteract cellular senescence rather than to support proliferation. PMID:25554796

  12. Proliferation networks at the time of targeted sanctions

    After having outlined that nuclear proliferation networks are using the existing international market, the authors present the terminology associated with this field (acquisition transaction, acquisition organization) and outline that these networks are made of natural and legal persons with the objective of acquiring goods related to arm programs. They also notice that these natural and legal persons may intervene in an unwittingly way. They comment the evolution of proliferation networks during the past ten years in relationship with the worldwide development of goods flow and export controls. They outline that control measures and mechanisms must be continuously adapted to the evolution of bypassing methods used by proliferation networks

  13. Scaffold architecture and fibrin gels promote meniscal cell proliferation

    Pawelec, K. M., E-mail: pawelec.km@gmail.com, E-mail: jw626@cam.ac.uk; Best, S. M.; Cameron, R. E. [Cambridge Centre for Medical Materials, Materials Science and Metallurgy Department, University of Cambridge, Cambridge CB3 0FS (United Kingdom); Wardale, R. J., E-mail: pawelec.km@gmail.com, E-mail: jw626@cam.ac.uk [Division of Trauma and Orthopaedic Surgery, Department of Surgery, University of Cambridge, Cambridge CB2 2QQ (United Kingdom)

    2015-01-01

    Stability of the knee relies on the meniscus, a complex connective tissue with poor healing ability. Current meniscal tissue engineering is inadequate, as the signals for increasing meniscal cell proliferation have not been established. In this study, collagen scaffold structure, isotropic or aligned, and fibrin gel addition were tested. Metabolic activity was promoted by fibrin addition. Cellular proliferation, however, was significantly increased by both aligned architectures and fibrin addition. None of the constructs impaired collagen type I production or triggered adverse inflammatory responses. It was demonstrated that both fibrin gel addition and optimized scaffold architecture effectively promote meniscal cell proliferation.

  14. The possibility of life proliferation from Enceladus

    Czechowski, Leszek

    2016-04-01

    Introduction: Enceladus is a medium-sized icy satellite (MIS) of Saturn. MIS are built of mixtures of rocks and ices. Enceladus with its radius of 250 km is one of the smallest of MIS, however, it is geologically active. According to [1]: "For life to have emerged […] on the early Earth, a sustained source of chemically transducible energy was essential. The serpentinization process is emerging as an increasingly likely source of that energy. Serpentinization of ultramafic crust would have continuously supplied hydrogen, methane, […] to off-ridge alkaline hydrothermal springs that interfaced with the metal-rich carbonic Hadean Ocean" (see also [2]). We consider here conditions for origin of life in early Enceladus and possible proliferation of the life from this satellite to the rest of Solar System. Mass of serpentinite: The serpentinization on the Earth is found in neovolcanic zones along mid-oceanic spreading centers. However, only in small part of them the hydrothermal activity really occurs. After [3] we consider the following reaction: Mg2SiO4 + MgSiO3 + 2H2O -> Mg3Si2O5(OH)4 . This reaction releases 241 000 J per kg of serpentine produced. Simple calculations (e.g. [4]) indicate that mass fraction of silicatesfmas in Enceladus is ˜0.646, hence the total mass of its silicate is ˜6.97 1019 kg. [4] found that the early core in Enceladus was a relatively cold structure built from loosely packed grains with water between them. At that time, there was not mechanism of removing the water. Since rocks are permeable up to the pressure of ˜300 MPa then the entire core of Enceladus was probably permeable for liquids. This could lead to formation of extensive hydrothermal convective systems. T-p conditions in Enceladus: The pressure in the center of Enceladus is ˜2.3 107 Pa that corresponds to pressure on the depth 2300 m in a terrestrial ocean. The evolution of temperature in the Enceladus interior for the first a few hundreds Myr is considered by [4]. If

  15. Regulation of apoptosis by peroxisome proliferators.

    Roberts, Ruth A; Michel, Cecile; Coyle, Beth; Freathy, Caroline; Cain, Kelvin; Boitier, Eric

    2004-04-01

    Peroxisome proliferators (PPs) constitute a large and chemically diverse family of non-genotoxic rodent hepatocarcinogens that activate the PP-activated receptor alpha (PPARalpha). In order to investigate the hypothesis that PPs elicit their carcinogenic effects through the suppression of apoptosis, we established an in vitro assay for apoptosis using both primary rat hepatocytes and the FaO rat hepatoma cell line. Apoptosis was induced by transforming growth factor beta1 (TGFbeta1), the physiological negative regulator of liver growth. In this system, PPs could suppress both spontaneous and TGFbeta1-induced apoptosis. In order to understand the mechanisms of this regulation of apoptosis, we conducted microarray analysis followed by pathway-specific gene clustering in TGFbeta1-treated cells. After treatment, 76 genes were up-regulated and 185 were down-regulated more than 1.5-fold. Cluster analysis of up-regulated genes revealed three clusters, A-C. Cluster A (4h) was associated with 12% apoptosis and consisted of genes mainly of the cytoskeleton and extracellular matrix such as troponin and the proteoglycan SDC4. In cluster B (8h; 25% apoptosis), there were many pro- and anti-apoptotic genes such as XIAP, BAK1 and BAD, whereas at 16h (40% apoptosis) the regulated genes were mainly those of the cellular stress pathways such as the genes implicated in the activation of the transcription factor NFkappab. Genes found down-regulated in response to TGFbeta1 were mainly those associated with oxidative stress and several genes implicated in glutathione production and maintenance. Thus, TGFbeta1 may induce apoptosis via a down regulation of oxidant defence leading to the generation of reactive oxygen species. The ability of PPs to impact on these apoptosis pathways remains to be determined. To approach this question, we have developed a technique using laser capture microdissection of livers treated with the PP, clofibric acid coupled with gene expression array analysis

  16. Inhibitory effect of CT domain of CCN3/NOV on proliferation and differentiation of osteogenic mesenchymal stem cells, Kusa-A1

    CCN3/NOV activates the Notch signal through the carboxyl terminal cysteine-rich (CT) domain. CCN3 transfection to Kusa-A1 inhibited osteogenic differentiation and cell proliferation, which is accompanied by upregulation of Hes/Hey, Notch downstream targets, and p21, a CDK inhibitor. Upregulation of Hes/Hey and p21 was abrogated by the deletion of CT domain. Anti-proliferative activity of CCN3 was also abrogated by CT domain deletion whereas anti-osteogenic activity was not completely abrogated. We found that CT domain-deleted CCN3 still possesses antagonistic effect on BMP-2. These results suggest that CCN3 employs Notch and BMP pathways in anti-osteogenic activity while it inhibits cell proliferation uniquely by Notch/p21 pathway

  17. TRPM7 channel regulates PDGF-BB-induced proliferation of hepatic stellate cells via PI3K and ERK pathways

    Fang, Ling, E-mail: fangling_1984@126.com; Zhan, Shuxiang; Huang, Cheng; Cheng, Xi; Lv, Xiongwen; Si, Hongfang; Li, Jun, E-mail: lj@ahmu.edu.cn

    2013-11-01

    TRPM7, a non-selective cation channel of the TRP channel superfamily, is implicated in diverse physiological and pathological processes including cell proliferation. Recently, TRPM7 has been reported in hepatic stellate cells (HSCs). Here, we investigated the contribution role of TRPM7 in activated HSC-T6 cell (a rat hepatic stellate cell line) proliferation. TRPM7 mRNA and protein were measured by RT-PCR and Western blot in rat model of liver fibrosis in vivo and PDGF-BB-activated HSC-T6 cells in vitro. Both mRNA and protein of TRPM7 were dramatically increased in CCl{sub 4}-treated rat livers. Stimulation of HSC-T6 cells with PDGF-BB resulted in a time-dependent increase of TRPM7 mRNA and protein. However, PDGF-BB-induced HSC-T6 cell proliferation was inhibited by non-specific TRPM7 blocker 2-aminoethoxydiphenyl borate (2-APB) or synthetic siRNA targeting TRPM7, and this was accompanied by downregulation of cell cycle proteins, cyclin D1, PCNA and CDK4. Blockade of TRPM7 channels also attenuated PDGF-BB induced expression of myofibroblast markers as measured by the induction of α-SMA and Col1α1. Furthermore, the phosphorylation of ERK and AKT, associated with cell proliferation, decreased in TRPM7 deficient HSC-T6 cells. These observations suggested that TRPM7 channels contribute to perpetuated fibroblast activation and proliferation of PDGF-BB induced HSC-T6 cells via the activation of ERK and PI3K pathways. Therefore, TRPM7 may constitute a useful target for the treatment of liver fibrosis. - Highlights: • Upregulation of TRPM7 mRNA and protein in the fibrotic livers from CCl{sub 4}-treated rats. • Increasing expression of TRPM7 mRNA and protein during HSC activation. • Blockade of TRPM7 inhibited the PDGF-BB induced proliferation of HSC-T6 cells. • Blockade of TRPM7 decreased α-SMA and Col1α1 expressions in activated HSC-T6 cells. • TRPM7 up-regulation contributes to the activation of ERK and AKT pathways.

  18. Proliferation of massive destruction weapons: fantasy or reality?; La proliferation des armes de destruction massive: fantasme ou realite?

    Duval, M

    2001-09-01

    This article evaluates the threat of massive destruction weapons (nuclear, chemical, biological) for Europe and recalls the existing safeguards against the different forms of nuclear proliferation: legal (non-proliferation treaty (NPT), comprehensive nuclear test ban treaty (CTBT), fissile material cut off treaty (FMCT) etc..), technical (fabrication of fissile materials, delays). However, all these safeguards can be overcome as proven by the activities of some countries. The situation of proliferation for the other type of massive destruction weapons is presented too. (J.S.)

  19. Nerve Regeneration Potential of Protocatechuic Acid in RSC96 Schwann Cells by Induction of Cellular Proliferation and Migration through IGF-IR-PI3K-Akt Signaling.

    Ju, Da-Tong; Liao, Hung-En; Shibu, Marthandam Asokan; Ho, Tsung-Jung; Padma, Viswanadha Vijaya; Tsai, Fuu-Jen; Chung, Li-Chin; Day, Cecilia Hsuan; Lin, Chien-Chung; Huang, Chih-Yang

    2015-12-31

    Peripheral nerve injuries, caused by accidental trauma, acute compression or surgery, often result in temporary or life-long neuronal dysfunctions and inflict great economic or social burdens on the patients. Nerve cell proliferation is an essential process to restore injured nerves of adults. Schwann cells play a crucial role in endogenous repair of peripheral nerves due to their ability to proliferate, migrate and provide trophic support to axons via expression of various neurotrophic factors, such as the nerve growth factor (NGF), especially after nerve injury. Protocatechuic acid (PCA) is a dihydroxybenzoic acid, a type of phenolic acid, isolated from the kernels of Alpinia oxyphylla Miq (AOF), a traditional Chinese herbal medicine the fruits of which are widely used as a tonic, aphrodisiac, anti-salivation and anti-diarrheatic. This study investigated the molecular mechanisms by which PCA induces Schwann cell proliferation by activating IGF-IR-PI3K-Akt pathway. Treatment with PCA induces phosphorylation of the insulin-like growth factor-I (IGF-I)-mediated phosphatidylinositol 3 kinase/serine - threonine kinase (PI3K/Akt) pathway, and activates expression of cell nuclear antigen (PCNA) in a dose-dependent manner. Cell cycle analysis after 18 h of treatment showed that proliferation of the RSC96 cells was enhanced by PCA treatment. The PCA induced proliferation was accompanied by modulation in the expressions of cell cycle proteins cyclin D1, cyclin E and cyclin A. Knockdown of PI3K using small interfering RNA (siRNA) and inhibition of IGF-IR receptor resulted in the reduction in cell survival proteins. The results collectively showed that PCA treatment promoted cell proliferation and cell survival via IGF-I signaling. PMID:26717920

  20. Dixdc1 targets CyclinD1 and p21 via PI3K pathway activation to promote Schwann cell proliferation after sciatic nerve crush.

    Wu, Weijie; Liu, Qingqing; Liu, Yuxi; Yu, Zhaohui; Wang, Youhua

    2016-09-16

    Dixdc1 (DIX domain containing-1), the mammalian homolog of Ccd1 (Coiled-coil-Dishevelled-Axin1), is a protein containing a coiled-coil domain and a Dishevelled-Axin (DIX) domain. As a novel component of the Wnt pathway, Dixdc1 has been reported to be able to promote neural progenitor proliferation and neuronal differentiation via Wnt/β-catenin signaling. But there still remains something unknown about Dixdc1 distribution and functions in the lesion and regeneration of the peripheral nervous system (PNS), so we tried to investigate dynamic changes of Dixdc1 expression in a rat sciatic nerve crush (SNC) model in this study. First of all, we detected SNC-induced increased levels of Dixdc1 in Schwann cells and interestingly identified parallel expression of PCNA (proliferation cell nuclear antigen) with Dixdc1. Besides, we observed up-regulated Dixdc1 during the process of TNF-α-induced Schwann cell proliferation. Also, we discovered that Dixdc1 could promote G1-S phase transition accompanied with the up-regulation of CyclinD1 and down-regulation of p21. More importantly, enhanced effects of Dixdc1 on cell proliferation were confirmed to be associated with PI3K activation. Not only blocking of the PI3K but Dixdc1 knockdown led to significantly decreased ability for proliferation, as well as down-regulation of CyclinD1 and up-regulation of p21. In summary, these data demonstrated that Dixdc1 might participate in Schwann cell proliferation by targeting CyclinD1 and p21 at least partially through the PI3K/AKT activation. PMID:27521891