UK PubMed Central (United Kingdom)

The primary aim of this study was to develop a model system that uses epidermal cells (keratinocytes and accessory pigmented cells) cultured on a reconstituted basement membrane biomatrix for use in...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

A major regulatory element required for expression of the human [alpha]-globin genes is located 40 kb upstream of the embryonic [zeta]-globin gene. To understand how this and other locus control region (LCR) elements contribute to high-level expression in erythroid cells, we have performed high-resolution, in vivo dimethyl sulfate footprinting. In addition, we have modified the dimethyl sulfate-based ligation-mediated polymerase chain reaction in vivo footprinting procedure to permit the assessment of interactions at guanine and adenine residues, rather than guanines alone. In vivo footprinting of the human [alpha]-LCR element carried on chromosome 16 in a mouse erythroleukemia cell environment revealed protein occupancy at GATA-1, AP-1/NF-E2, and CACC/GGTGG motifs, specific differences compared with in vitro protein binding, and distinct changes in one region upon dimethyl sulfoxide-induced cellular maturation. No protein ...

CERN Document Server

Textile machinery and accessories -- Bearings for bottom rollers and allied dimensions -- Caps with central nose and caps with side lugs

UK PubMed Central (United Kingdom)

Accessory cusp present on the occlusal surface may seldom pose problems. While its presence may not be a cause for alarm in most instances, it can sometimes lead to serious consequences if it is damaged....Full Text Available

Science.gov (United States)

No abstract prepared.

UK PubMed Central (United Kingdom)

Endothelial cells and mural cells (smooth muscle cells, pericytes, or fibroblasts) are known to communicate with one another. Their interactions not only serve to support fully functional blood...Full Text Available

Science.gov (United States)

This paper provides a snapshot of the computer network security industry and addresses specific issues related to network security in public education. The following topics are covered: (1) security policy, including reasons for establishing a policy, risk assessment, areas to consider, audit tools; (2) workstations, including physical security, protecting workstation components, and computer viruses; (3) the local network, including the OSI (Open Systems Interconnection) reference model, protocols, network segmentation, network management, network sniffing, and data encryption; (4) servers, including UNIX and other server operating systems; (5) remote access, including technologies, remote access servers, protocols, and authentication/authorization; (6) crackers and hackers, including threats and hacking tools/techniques; (7) Internet firewalls, including functions, issues and problems, types, rules, logs, firewall accessories, buying a firewall, and firewall ...

UK PubMed Central (United Kingdom)

Diverse cell polarity networks require positive feedback for locally amplifying distributions of signalling molecules at the plasma membrane1. Additional...Full Text Available

UK PubMed Central (United Kingdom)

Although most self-reactive T cells are eliminated in the thymus, mechanisms to inactivate or control T cells specific for extrathymic antigens are required and exist in the periphery. By investigating...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundA fundamental requirement for the exploitation of embryonic stem (ES) cells in regenerative medicine is the ability to reproducibly derive sufficient numbers of cells of...Full Text Available

International Nuclear Information System (INIS)

Larsen syndrome is an autosomal-dominant disorder characterized by multiple joint dislocations, vertebral anomalies and dysmorphic facies. Both autosomal-dominant and autosomal-recessive forms of the disorder have been proposed. Individuals with autosomal-dominant Larsen syndrome have characteristic ''cylindrical-shape'' thumbs caused by broad, shortened phalanges. Autosomal-dominant Larsen syndrome results from heterozygosity for mutations in filamin B, a cytoskeletal protein involved in multicellular processes. We report here a patient with a duplicated or accessory distal thumb phalanx and multiple large joint dislocations who was shown to be heterozygous for a filamin B mutation predicting the amino acid substitution G1691S. This adds a new radiographic finding, duplicated or accessory distal phalanx, to the radiographic abnormalities seen in this rare dominant disorder. (orig.)

Energy Technology Data Exchange (ETDEWEB)

Following an earlier article on developments to enhance the reliability of supertension oil filled cables and accessories, changes are described in design and installation of complete cable systems which have similarly improved operating performance.

UK PubMed Central (United Kingdom)

The Neurofibromatosis-2 (NF2) tumor suppressor merlin negatively regulates cell proliferation in numerous cell types. We have previously shown that the NF2...Full Text Available

UK PubMed Central (United Kingdom)

The ubiquitin–proteasome pathway is the principal system for extralysosomal protein degradation in eukaryotic cells, and is essential for the regulation and maintenance of basic cellular processes,...Full Text Available

UK PubMed Central (United Kingdom)

Auxin autonomous growth of most crown gall tumor cells requires the expression of two auxin biosynthesizing genes (tms 1 and tms 2) from the T-DNA of Agrobacterium...Full Text Available

UK PubMed Central (United Kingdom)

ATP-binding cassette A3 (ABCA3) is a lipid transport protein required for synthesis and storage of pulmonary surfactant in type II cells in the alveoli. Abca3 was conditionally deleted...Full Text Available

International Nuclear Information System (INIS)

A Siemens Mevatron KV2 accelerator installed at the Royal Adelaide Hospital employs cylindrical solid-walled electron cones for some electron collimation. The cones being used at present result in treatment fields that do not always conform with the International Electrotechnical Commission (IEC) Standards (particularly at high energies). The aim of this project was to simulate the existing cones using Monte Carlo methods in order to evaluate potential cone modifications required to overcome the field irregularities. Simulations were performed using the EGS4 (Electron Gamma Shower version 4, distribution II) Monte Carlo code installed on a DEC Alpha workstation at the University of South Australia. To rigorously simulate the existing electron cones it was necessary to also simulate various components within the treatment head of the linear accelerator. Results of simulations for existing cones were found to be consistent with experimental data. (obtained from Royal ...

UK PubMed Central (United Kingdom)

Myriad genetic and epigenetic alterations are required to drive normal cells toward malignant transformation. These somatic events commandeer many signaling pathways that cooperate to endow aspiring...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

An assay system for the stem cell that colonizes the thymus and differentiates into T cells was developed, and by using this assay system the existence of two subpopulations of stem cells for T cell lineage was clarified. Part-body-shielded and 900-R-irradiated C57BL/6 (H-2b, Thy-1.2) recipient mice, which do not require the transfer of pluripotent stem cells for their survival, were transferred with cells from B10 X Thy-1.1 (H-2b, Thy-1.1) donor mice. The reconstitution of the recipient's thymus lymphocytes was accomplished by stem cells in the donor cells and those spared in the shielded portion of the recipient that competitively colonize the thymus. Thus, the stem cell activity of donor cells can be evaluated by determining the proportion of ...

British Library Electronic Table of Contents (United Kingdom)

Colorectal cancer is the second most common cause of cancer death in the world and about half of the patients with colorectal cancer require adjuvant therapy after surgical resection. Therefore, the eradication of cancer cells via chemotherapy constitutes a viable approach to treating patients with colorectal cancer. In this study, the effects of bufalin isolated from a traditional Chinese medicine were evaluated and characterized in HT-29 and Caco-2 human colon cancer cells. Contrary to its well-documented apoptosis-promoting activity in other cancer cells, bufalin did not cause caspase-dependent cell death in colon cancer cells, as indicated by the absence of significant early apoptosis as well as poly(ADP-ribose) polymerase and caspase-3 cleavage. Instead, bufalin activated an autophagy...

UK PubMed Central (United Kingdom)

Angiotensin II (Ang II) is a key proapoptotic factor in fibrotic tissue diseases. However, the mechanism of Ang-II-induced cell death in endothelial cells has not been previously elucidated....Full Text Available

Energy Technology Data Exchange (ETDEWEB)

This paper describes the development of cellulose paper/plastic film/cellulose paper laminate insulated Self-Contained 500 kV Oil-Filled (OF) Cable and its accessories. Dielectric loss of this new cable was confirmed to be about one third that of conventional kraft paper cable. Moreover, by employing the reduced insulation thickness of 25 mm rather than that of 33 mm, as is usual with conventional cables, the new cable could be made very compact, due to the high dielectric strengths of laminated paper. As a result, the shipping reel length of the new cable, which is about 10% lighter in total weight and 10% smaller in overall diameter than conventional cables, could be dramatically increased. The reliability and the stability of the new cable have been demonstrated by the successful completion of a one and a half year field test.

Energy Technology Data Exchange (ETDEWEB)

The instrument ball bearings are the key components of movable components for various kinds of measuring and control instruments; they often operate in the environmental condition of high-speed and light preload. In general, the non metal, disposable oilimpregnated retainer material has been used for these kinds of high precision miniature bearing. The engineering practice shows that the common failure mode of them is the wear which appears under the condition of insufficient lubrication condition. As the results, the vibration and noise will be enlarged, so does the frictional torque, which makes the ball bearings to lose its original working accuracy. It is the lifetime test of bearings that can enable the designers and manufacturers to chose the material of the bearing properly, optimize the product structure, mend the manufacturing technique process, and to enhance the technical level of the bearing products significantly. In this paper, the wear lifetime tester has been designed ...

Energy Technology Data Exchange (ETDEWEB)

Extracellular ATP stimulates transmembrane ion fluxes in the mouse macrophage cell line J774. In the presence of Mg2+, nonhydrolyzable ATP analogs and other purine and pyrimidine nucleotides do not elicit this response, suggesting the presence of a specific receptor for ATP on the macrophage plasma membrane. One candidate for such a receptor is the ecto-ATPase expressed on these cells. We, therefore, investigated the role of this enzyme in ATP-induced /sup 86/Rb+ efflux in J774 cells. The ecto-ATPase had a broad nucleotide specificity and did not hydrolyze extracellular ATP in the absence of divalent cations. /sup 86/Rb+ efflux was not blocked by inhibition of the ecto-ATPase and did not require Ca2+ or Mg2+. In fact, ATP-stimulated /sup 86/Rb+ efflux was inhibited by Mg2+ and correlated with the availability of ATP4- in the medium. In the absence of divalent cations, the slowly hydrolyzable ATP analogs ...

International Nuclear Information System (INIS)

Swelling hemoglobin CC erythrocytes stimulates a ouabain-insensitive K flux that restores original cell volume. Studies were performed with the K analog, "8"6Rb. This volume regulatory pathway was characterized for its anion dependence, sensitivity to loop diuretics, and requirement for Na. The swelling-induced K flux was eliminated if intracellular chloride was replaced by nitrate and both swelling-activated K influx and efflux were partially inhibited by 1 mM furosemide or bumetanide. K influx in swollen hemoglobin CC cells was not diminished when Na in the incubation medium was replaced with choline, indicating Na independence of the swelling-induced flux. Identical experiments with hemoglobin AA cells also demonstrated a swelling-induced increase in K flux, but the magnitude and duration of this increase were considerably less than that seen with hemoglobin CC cells. The ...

UK PubMed Central (United Kingdom)

The rapidly activating and inactivating voltage-dependent outward K⁺ (Kv) current, I_A, is widely expressed in central and peripheral neurons. I_A has long been...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

Demidospermus annulus sp. nov. (Platyhelminthes, Monogenea, Ancyrocephalidae) is described from the gills of the catfish Parapimelodus valenciennis L?tken collected in Samboromb?n Bay, Argentina. The new species differs from all congeneric species mainly by the structure of the accessory piece of the male copulatory organ, the sclerotized ring-shaped vaginal aperture and the dorsal bar articulation.

Science.gov (United States)

Stem cell research has important implications for medicine. The source of stem cells influences their therapeutic potential, with stem cells derived from early-stage embryos remaining the most versatile. Somatic cell nuclear transfer (SCNT), a source of embryonic stem cells, allows for understandings about disease development and, more importantly, the ability to yield embryonic stem cell lines that are genetically matched to the somatic cell donor. However, SCNT requires women to donate eggs, which involves injection of ovulation-inducing hormones and egg retrieval through laparoscopy or transvaginal needle aspiration. Risks from this procedure are fiercely debated, most notably risk of ovarian hyperstimulation syndrome (OHSS). This review examines risk of OHSS resulting from oocyte donation. We conclude that risk posed ...

UK PubMed Central (United Kingdom)

Transient receptor potential (TRP) ion channels have been implicated in detecting chemical, thermal and mechanical stimuli in organisms ranging from mammals to Caenorhabditis elegans....Full Text Available

UK PubMed Central (United Kingdom)

Normal brain development requires a series of highly complex and interrelated steps. This process presents many opportunities for errors to occur, which could result in developmental defects...Full Text Available

UK PubMed Central (United Kingdom)

The study of blocking factors requires in vitro assay of cell mediated immunity that parallels the in vivo response. By microcytotoxicity testing, progressor and immune peripheral blood lymphocytes...Full Text Available

UK PubMed Central (United Kingdom)

A procedure was developed to isolate polyribosomes from plant cell cultures. Tobacco callus extracted in 10 mm MgCl₂, 80 mm KCl, 250 mm sucrose, and 140 mm...Full Text Available

UK PubMed Central (United Kingdom)

The formation of mammalian secondary palate requires a series of developmental events such as growth, elevation and fusion. Despite recent advances in the field of palate development, the process...Full Text Available

UK PubMed Central (United Kingdom)

SUMMARYMotile dendritic filopodial processes are thought to be precursors of spine synapses, but how motility relates to cell-surface cues required for axon-dendrite recognition...Full Text Available

UK PubMed Central (United Kingdom)

Cyclophilins (Cyps), the intracellular receptors for Cyclosporine A (CsA), are responsible for peptidyl-prolyl cis-trans isomerisation and for chaperoning several membrane proteins. Those functions...Full Text Available

UK PubMed Central (United Kingdom)

Motor function requires that motor axons extend from the spinal cord at regular intervals and that they are myelinated by Schwann cells. Little attention has been given to another cellular structure,...Full Text Available

UK PubMed Central (United Kingdom)

Oncolytic viruses (OVs) represent a relatively novel anti-cancer modality. Like other new cancer treatments, effective OV therapy will likely require combination with conventional treatments. In order...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundGATA-2 is a transcription factor required for hematopoietic stem cell survival as well as for neuronal development in vertebrates. It has been shown that specific expression...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

For optimal performances, proton exchange membrane fuel cells require fine water and thermal management. Accurate modelling of the physical phenomena occurring in the fuel cell is a key issue to improve fuel cell technology. Here, an analytic steady state diphasic 2D model of heat and mass transfer is presented. Through this model, the aim of this work is to study the influence of local events on the global performances of a fuel cell. A part of the complete model is a microscopic representation of the coupling between water transport and charge transfers in the electrodes. The thickness of the liquid layer around the reactive agglomerates is deduced from the saturation. The evolution of the quantity of water within the catalyst layer is monitored and its influence on the global performanc...

Energy Technology Data Exchange (ETDEWEB)

A technique for delivering genes carried by recombinant retroviruses into specific cell types could have numerous applications in oncology, developmental biology, and gene therapy. As a first step toward this remote goal the authors designed a procedure allowing in vitro cell targeting by retroviruses. Biotinylated antibodies against the viral envelope protein on one side, and against specific cell membrane markers on the other side, were bridged by streptavidin and used to link the virus to the host. The method was successfully used to infect human cells with ecotropic murine retroviruses by means of major histocompatibility complex class I and class II antigens and appears easily adaptable to other cell, membrane markers. Moreover, the sequential protocol they design, although allowing infection of human cells, requires less stringent ...

UK PubMed Central (United Kingdom)

P2X1 receptors for ATP contribute to signalling in a variety of cell types and following stimulation undergo rapid desensitisation (within 1 s), and require ∼5 min to recover. In HEK293 cells...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

One of the first technical applications of gene technology is reported here. The DNA of E. coli cells was rearranged such that these cells produce large quantities of the enzyme ..cap alpha..-galactosidase. This enzyme is used to split the trisaccharide, raffinose, which is formed during the production of sugar from sugar beet. By this means sugar yields can be increased and the energy required for sugar crystallisation reduced.

DEFF Research Database (Denmark)

The evolution of "humanized" (i.e., free of animal sourced reagents) and ultimately chemically defined culture systems for human embryo stem cell (hESC) isolation and culture is of importance to improving their efficacy and safety in research and therapeutic applications. This can be achieved by integration of a multitude of individual approaches to replace or eliminate specific animal sourced reagents into a single comprehensive protocol. In the present study our objective was to integrate strategies obviating reliance on some of the most poorly defined and path-critical factors associated with hESC derivation, namely the use of animal immune compliment to isolate embryo inner cell mass, and animal sourced serum products and feeder cells to sustain hESC growth and attachment. As a result we report the derivation of six new hESC lines isolated by outgrowth from whole blastocysts on an extracellular matrix substrate of ...

UK PubMed Central (United Kingdom)

The yeast phosphatidylinositol transfer protein (Sec14p) is required for biogenesis of Golgi-derived transport vesicles and cell viability, and this essential Sec14p requirement is abrogated by inactivation...Full Text Available

Science.gov (United States)

One of the NASA research activities was to identify, characterize, and simulate a series of technologies that could be used for hydrogen production at NASA Kennedy Space Center (KSC) using locally available sources. This project examined the production of hydrogen from solar energy. To produce hydrogen by water splitting, the operating voltage of conventional photovoltaic (PV) cells cannot supply the overvoltage required. Thus, the objective of this project was to research and develop photoelectrochemical (PEC) cells that can supply the required voltage for water splitting by constructing a multiple bandgap tandem PV cell and a photocatalyst that can be activated by infrared (IR) photons transmitted through the PV cell. The proposed concept is different from conventional PEC water splitting by using multiple band gap combinations. The advantages for this PEC ...

Energy Technology Data Exchange (ETDEWEB)

Computer simulations of two-junction, concentrator tandem solar cell performance show that IR-sensitive bottom cells are required to achieve high efficiencies. Based on this conclusion, two novel concentrator tandem designs are under investigations: (1) a mechanically stacked, four-terminal GaAs/GaInAsP (0.95 eV) tandem, and (2) a monolithic, lattice-matched, three-terminal InP/GaInAs tandem. In preliminary experiments, terrestrial concentrator efficiencies exceeding 30% have been achieved with each of the above tandem designs. Methods for improving the efficiency of each tandem type are discussed. (orig.).

Energy Technology Data Exchange (ETDEWEB)

All power plant engineers face the problem of peak power demands. Pumped storage plants are used to generate peak load power by pumping up water utilizing off-peak energy of hydrothermal and thermonuclear plants. This is the first accessible text/reference to cover hydroelectric power generation with emphasis on engineering to meet peak power demands by means of pumped storge plants, tidal power plants, and low head power generation. Text covers hydrology, mechanical and electrical equipment, accessories such as penstocks and valves, and civil engineering considerations. Contains descriptions of several existing plants. Includes 200 diagrams and 50 photographs.

Energy Technology Data Exchange (ETDEWEB)

The ElectroScan environmental scanning electron microscope (ESEM) is one of the most exciting new developments in the field of Electron Microscopy. The ESEM differs from conventional Scanning Electron Microscopes (SEM) by being able to examine materials including liquids and oils in their natural state with no prior sample preparation. Accessory equipment, cooling, heating and manipulating devices allow the manipulation of samples thus making it possible for the first time to image dynamic processes such as wetting, drying, absorption, corrosion, melting, crystallisation, curing and fracturing at high magnification. Papers concerning the historical development of the ESEM are given in a bibliography at the end of this paper. 24 refs., 18 figs.

International Nuclear Information System (INIS)

Presently, Nigeria is one of the fastest growing Telecom markets in the world. The country's teledensity increased from a mere 0.4 in 1999 to 10 in 2005 following the liberalization of the Telecom sector in 2001. More than 25 million new digital mobile lines have been connected by June 2006. Large quantities of mobile phones and accessories including secondhand and remanufactured products are being imported to meet the pent-up demand. This improvement in mobile telecom services resulted in the preference of mobile telecom services to fixed lines. Consequently, the contribution of fixed lines decreased from about 95% in year 2000 to less than 10% in March 2005. This phenomenal progress in information technology has resulted in the generation of large quantities of electronic waste (e-waste) in the country. Abandoned fixed line telephone sets estimated at 120,000 units are either disposed or stockpiled. Increasing quantities of waste mobile phones estimated at 8 ...

Energy Technology Data Exchange (ETDEWEB)

Exogenous adenosine 5{prime}-triphosphate (ATP) mobilized intracellular calcium in human carcinoma A43l cells and in Swiss 3T3 and 3T6 mouse fibroblasts by increasing inositol trisphosphate similar to well down growth factors (platelet-derived growth factor (PDGF), epidermal growth factor (EGF), bradykinin (BK), serum). Calcium mobilization was examined by video imaging of fura-2 fluorescence is single cells, following the radioactive isotope {sup 45}Ca, and monitoring the decrease in fluorescence of cells loaded with chlortetracycline. Uridine 5{prime}-triphosphate, but not other nucleotides, mimicked ATP. Single-cell analysis revealed synchronous responses in 10 sec to ATP, BK or serum, while PDGF (3T3) and EGF (A431) produced slower signals with significant cell-to-cell variation. PDGF desensitized 3T3 cells to ATP and BK added 100 sec later but ATP or BK did ...

International Nuclear Information System (INIS)

Nickel cadmium batteries differ greatly in their mechanical design and construction of the electrodes. Using available electrode constructions, batteries are designed which meet the requirements of specific applications and offer optimum performance. Pocket- and tubular cells are basically developed with the technology of the year 1895. Since then some improvements with todays technology have been made. The sintered cells use the technology of the 1930's and they are still limited to high power application. With this knowledge and the technology of today the fiber-structured nickel electrode (FNC) was developed at DAUG laboratory, a subsidiary company of Mercedes-Benz and Volkswagen. After ten years of experience in light weight prototype batteries for electric vehicles (1-2), the system was brought into production by a new company, DAUG-HOPPECKE. Characteristics of fiber electrodes: thickness and size can be easily ...

International Nuclear Information System (INIS)

To investigate the growth inhibitory effects, and the underlying mechanism of human colon cancer cell (HT-29) death, induced by a new synthetic bile derivative (HS-1200). Human colon cancer cells (HT-29), in exponential growth phase, were treated with various concentrations of a new synthetic bile acid derivative (HS-1200). The growth inhibitory effects on HT-19 cells were examined using a trypan blue exclusion assay. The extent of apoptosis was determined using agarose gel electrophoresis, TUNEL assays and Hoechst staining. The apoptotic cell death was also confirmed by Western blotting of PARP, caspase-3 and DNA fragmentation factor (DFF) analysis. To investigate the involvement of mitochondria, we employed immunofluorescent staining of cytochrome c and mitochondrial membrane potential analyses. The dose required for the half maximal inhibition (IC_5_0) of the HT-29 ...

International Nuclear Information System (INIS)

The efficiency of electric power generation by dye-sensitized mesoscopic photovoltaic cells has been progressing steadily over the last years reaching now 11% in full sunlight. An important question for practical applications concerns the stability of these devices under prolonged exposure to light or heat. Strikingly stable operation can be obtained by judicious selection of the sensitizer, electrolyte and sealant rendering feasible a service life of at least 20 years under normal outdoor conditions. The sensitizer playing a central role in the light energy conversion process, we analyze the kinetic requirements for it to sustain the required one hundred million turnovers. We also review recent results on the use of self-assembled monolayers of amphiphilic sensitizers and co-adsorbents to enhance the thermal robustness of the device. (author)

British Library Electronic Table of Contents (United Kingdom)

Glass ceramics are commonly used as sealing materials for planar solid oxide fuel cells (SOFCs). The major requirements of stack and module builders for these materials are the stability of the coefficient of thermal expansion (CTE), excellent bonding (sticking) behaviour and the absence of volatile ingredients, which can lead to changes of the material properties and the sealing ability. SCHOTT Electronic Packaging has developed special glasses and glass-ceramics for various solid oxide fuel cell designs and operating temperatures. The glass compositions are based on the system MgO-Al2O3-BaO-SiO2-B2O3. In this study the evaluation of the developed materials was done by high temperature aging tests for up to 1000h, high temperature XRD-studies and Rietveld calculations, combined with scann...

Energy Technology Data Exchange (ETDEWEB)

This report summarizes the work performed by Honeywell during the October 2001 to December 2001 reporting period under Cooperative Agreement DE-FC26-01NT40779 for the U. S. Department of Energy, National Energy Technology Laboratory (DOE/NETL) entitled ''Solid Oxide Fuel Cell Hybrid System for Distributed Power Generation''. The main objective of this project is to develop and demonstrate the feasibility of a highly efficient hybrid system integrating a planar Solid Oxide Fuel Cell (SOFC) and a turbogenerator. The conceptual and demonstration system designs were proposed and analyzed, and these systems have been modeled in Aspen Plus. Work has also started on the assembly of dynamic component models and the development of the top-level controls requirements for the system. SOFC stacks have been fabricated and performance mapping initiated.

Energy Technology Data Exchange (ETDEWEB)

Hybrid electric vehicles (HEV) need long-lived high-power batteries as energy storage devices. Batteries based on lithium-ion technology can meet the high-power goals but have been unable to meet HEV calendar-life requirements. As part of the US Department of Energy's Advanced Technology Development (ATD) Program, diagnostic studies are being conducted on 18650-type lithium-ion cells that were subjected to accelerated aging tests at temperatures ranging from 40 to 70 C. This article summarizes data obtained by gas chromatography, liquid chromatography, electron microscopy, X-ray spectroscopy and electrochemical techniques, and identifies cell components that are responsible for the observed impedance rise and power fade.

Energy Technology Data Exchange (ETDEWEB)

This paper explores the potential of solid oxide fuel cells (SOFCS) as 3--10 kW auxiliary power units for trucks and military vehicles operating on diesel fuel. It discusses the requirements and specifications for such units, and the advantages, challenges, and development issues for SOFCS used in this application. Based on system design and analysis, such systems should achieve efficiencies approaching 40% (lower heating value), with a relatively simple system configuration. The major components of such a system are the fuel cell stack, a catalytic autothermal reformer, and a spent gas burner/air preheater. Building an SOFC-based auxiliary power unit is not straightforward, however, and the tasks needed to develop a 3--10 kW brassboard demonstration unit are outlined.

British Library Electronic Table of Contents (United Kingdom)

The levels of General Transcription Factor (TF) IIA were examined during mammalian brain development and in rat embryo fibroblasts and transformed cell lines. The large TFIIA subunit paralogues ab and t are largely produced in unsynchronized cell lines, yet only TFIIA ab is observed in a number of differentiated tissue extracts. Steady-state protein levels of the TFIIA t, ab, and g subunits were significantly reduced when human embryonal (ec) and hepatic carcinoma cell lines were stimulated to differentiate with either all-trans-retinoic acid (ATRA) or sodium butyrate. ATRA-treated NT2-ec cells required replating to induce a neuronal phenotype and loss of detectable TFIIA t and g proteins. High levels of TFIIA t, ab, and g and Sp factors were identified in extracts from human fetal and rat...

Science.gov (United States)

We have used a cell-free polymerase I transcription system derived from HeLa cells to study the regulation of human rRNA synthesis. Analysis of deletion mutants spanning the start site of transcription at nucleotide +1 indicates that the control region affecting initiation of human rRNA synthesis is contained within sequences from nucleotides -158 to +18. This promoter region can be subdivided into (i) a central segment of approximately 40 base pair that is required for transcription and (ii) flanking sequences that influence the efficiency of transcription in vitro. We have examined the in vitro transcriptional activity of the human extract under various conditions that are thought to modulate rRNA synthesis in vivo. Cell-free extracts prepared from HeLa cells infected with adenovirus 2 synthesize human rRNA at levels greatly decreased relative to uninfected ...

Science.gov (United States)

The specification describes a semiconductor solar cell and fabrication process therefor wherein a thin N-type gallium arsenide layer is deposited on a larger P-type substrate layer which is selected from the group of III-V ternary compounds consisting of aluminum phosphide antimonide, AlPSb, and aluminum indium phosphide, AlInP. P-type impurities are diffused from the substrate layer into a portion of the thin N-type gallium arsenide layer to form P-type region wherein which defines a PN junction in the thin gallium arsenide layer. Thus, the quantity of gallium arsenide required to provide this PN photovoltaic junction layer in the cell is minimized, and th P-type substrate serves as a high bandgap window layer for the cell. Such high bandgap of this window material is especially well suited for efficiently transmitting the blue spectrum of sunlight to the PN junction, thus enhancing the power ...

Energy Technology Data Exchange (ETDEWEB)

The thermophotovoltaic (TPV) option was not selected for further deep space mission technology development in NASA for several reasons. Chief among them was the large radiator required to keep the photovoltaic cells at a sufficiently low operating temperature. This led to significant integration problems with the spacecraft and limited sensor view angles. It is clear that the issue of cell temperature is crucial for space applications because of radiator size and system impact. Many efforts have focused on matching cell band gap to appropriate emitters in the 1 to 2 {mu}m range, resulting in band gaps in the 0.5 to 0.8 eV range. However, low band gaps lead to low open circuit voltages ({approximately}0.25 to 0.45 V) caused by high intrinsic carrier concentrations (n{sub i}{sup 2}). Thus, in order to obtain high performance. Photovoltaic cell temperatures must be kept near room ...

British Library Electronic Table of Contents (United Kingdom)

Within the current outlook for sustainable electric energy supply with concomitant reduction in emission of greenhouse gases, accelerated attention is focusing on the long-term development of hydrogen fuel cell and all-electric battery vehicles to provide alternative fuels to replace petroleum-derived fuels for automotive national fleets. The potential varies significantly between large industrially developed nations and smaller industrially developing nations. The requirement for additional electric energy supply from low-specific energy renewable resources and high-specific energy nuclear resources depends strongly on individual national economic, environmental, and political factors. Analysis of the additional electric energy supply required for the two potential large-scale technologie...

UK PubMed Central (United Kingdom)

Werner syndrome (WS) is a rare disorder characterized by the premature onset of several pathologies associated with aging. The gene responsible for WS codes for a RecQ-type DNA helicase and is believed...Full Text Available

UK PubMed Central (United Kingdom)

The 37/67 kDa laminin receptor (LAMR) is a multifunctional protein, acting as an extracellular receptor, localizing to the nucleus, and playing roles in rRNA processing and ribosome assembly....Full Text Available

International Nuclear Information System (INIS)

One of several methods to build YSZ electrolyte of SOFC is plasma spraying. This system heats particle to a melting point and then pushing them into target and forming a deposition. This paper presents heating studies of YSZ particle in plasma jet. By simple model it is shown that the required time such those YSZ particle with grain size of 50 ?m to be melt is around 12 x 10-4 second. (author)

Energy Technology Data Exchange (ETDEWEB)

The purpose of the project is to further develop a Danish natural gas reformer system including optimisation of subsystems and the overall system consisting of a natural gas reformer and fuel cell CHP generator. This will contribute to the evaluation of to what extend Denmark shall develop small reformer units for PEM fuel Cells. In the project a reformer system with a high degree of automatic control has been build that fulfils the CHP requirements to operation time, dynamics etc. This work, with a FP05 reformer unit, has given valuable results concerning the possibilities and limitations of the reformer technology for CHP usage. It is important that the reformer and fuel cell units are designed with matching yields to optimise efficiency, turn-down start-up time etc. The burner that delivers heat for the steam reaction shall be able to use natural gas as fuel. This gives the possibility of using ...

International Nuclear Information System (INIS)

The effect of X-irradiation on production of MUC1 was studied with human colon carcinoma HT-29 cells. As evaluated by immunocytochemical staining, the percentages or MUC1-positive cells in cells at 4 days after 6 Gy irradiation and in unirradiated control cells were 52#+-#3.5% (n=6) and 26#+-#2.8% (n=6), respectively. Flow-cytometric analysis of living cells showed that MUC1 began to rise from day 1, reaching a plateau by day 4 after 6 Gy irradiation. Western blot analysis with monoclonal antibody MY.1E12 against glycosylated MUC1 (mature form) showed dose-dependent increases of two bands (500 and 390 kDa) corresponding to two polymorphic MUC1 alleles. Premature forms of MUC1 (350 and 240 kDa) were detectable with monoclonal antibody HMFG-2 only in irradiated cells, suggesting that new core protein synthesis had been induced. The transcriptional activity of the ...

Energy Technology Data Exchange (ETDEWEB)

Cdc7 kinase plays an essential role in firing of replication origins by phosphorylating components of the replication complexes. Cdc7 kinase has also been implicated in S phase checkpoint signaling downstream of the ATR and Chk1 kinases. Inactivation of Cdc7 in yeast results in arrest of cell growth with 1C DNA content after completion of the ongoing DNA replication. In contrast, conditional inactivation of Cdc7 in undifferentiated mouse embryonic stem (ES) cells leads to growth arrest with rapid cessation of DNA synthesis, suggesting requirement of Cdc7 functions for continuation of ongoing DNA synthesis. Furthermore, loss of Cdc7 function induces recombinational repair (nuclear Rad51 foci) and G2/M checkpoint responses (inhibition of Cdc2 kinase). Eventually, p53 becomes highly activated and the cells undergo massive p53-dependent apoptosis. Thus, defective origin activation in mammalian ...

British Library Electronic Table of Contents (United Kingdom)

Background. To determine total upper limb function following selective neck dissection over a mean follow-up of 1.6 years. Methods. A retrospective questionnaire study in a tertiary head and neck surgical unit. One hundred forty-eight patients who underwent selective neck dissection for head and neck cancer from January 2000 to December 2005 were invited to participate. The main outcome measure was ipsilateral upper limb dysfunction as measured by the Disability of Arm, Shoulder and Hand (DASH) questionnaire. Results. Sixty-five patients responded to the invitation to join the study from 148 invited. Despite accessory nerve conserving surgery for all the selective neck dissections studied, 23% reported no upper limb dysfunction, 54% reported mild upper limb dysfunction, 15% reported modera...

British Library Electronic Table of Contents (United Kingdom)

Complex I (NADH:ubiquinone oxidoreductase) is the largest enzyme of the mitochondrial respiratory chain. Compared to its bacterial counterpart which encompasses 14-17 subunits, mitochondrial complex I has almost tripled its subunit composition during evolution of eukaryotes, by recruitment of so-called accessory subunits, part of them being specific to distinct evolutionary lineages. The increasing availability of numerous broadly sampled eukaryotic genomes now enables the reconstruction of the evolutionary history of this large protein complex. Here, a combination of profile-based sequence comparisons and basic structural properties analyses at the protein level enabled to pinpoint homology relationships between complex I subunits from fungi, mammals or green plants, previously identified...

International Nuclear Information System (INIS)

"9"9"mTechnetium -heat damaged erythrocyte were used as spleen scanning agents in 12 patients from July, 1985 to April, 1986. We used this scan to evaluate situs inversus, asplenia, accessory spleen, hypersplenism, splenic infarction, tumor staging and evaluation of therapy, especially when the "9"9"mTc-tin colloid scans were not definite for diagnosis. The techniques applied to these scans were in vivo/in vitro-labeling method and heating-method to damage the erythrocytes. Liver-to-spleen uptake ratios were increased upto 100:1 and interference from the left lobe of the liver was eliminated. These scans were helpful to evaluate the spleen. (Author).

International Nuclear Information System (INIS)

Many types of maintenance activities are performed on contaminated (radioactive) machines and components at the Fast Flux Test Facility (FFTF). When personnel dose levels are within permissible limits, maintenance is performed in contaminated work areas or in gloveboxes. Otherwise, maintenance is performed in the Interim Examination and Maintenance (IEM) Cell, a hot cell that is used to perform tasks that require remote operation. Maintenance is often a combination of manual and remote operation. One such operation was the cleaning of the closed loop ex-vessel machine (CLEM) core component grapple (CCG). The CLEM is used to transfer core components in and out of the reactor vessel. The CCG had not been operating properly due to buildups of metallic sodium and sodium compounds on the outside surface and internal moving parts. This paper tells how the CCG was disassembled and cleaned. Part of this task was done in a glovebox ...

Energy Technology Data Exchange (ETDEWEB)

Solar High Altitude Powered Platform (Solar HAPP) aircraft are unmanned remote sensing vehicles designed for cruises lasting up to one year at 20-km altitude, while carrying up to 250 pounds of cameras and electrooptic sensors in an underslung payload pod. It is anticipated that real time IR and UV images of earth features may be more inexpensively and accurately obtained by this means than by the conventional geosynchronous earth resources satellites. Solar HAPPs, with wing spans of over 300 ft and weights of only 2000 lb, require ultralight composite structures with external wire bracing. Solar cells will cover both sides of the vertical wing stabilizers and wing tips, which hinge up in daytime to capture the maximum amount of sunlight. A 15-hp electric propulsion unit drives a low-rpm, large diameter propeller; power will be derived from the solar cells diurnally, and from hydrogen-oxygen fuel cells ...

Science.gov (United States)

Cell-penetrating peptides (CPPs) are useful tools for the delivery of hydrophilic bioactive molecules, such as peptides, proteins, and oligonucleotides, across the cell membrane. To realize the delivery of therapeutic macromolecules by CPPs, the CPPs are required to show resistance to protease and no cytotoxicity. In order to produce potent non-toxic and protease-resistant CPPs with high cellular uptake, we designed an amphipathic helix peptide using ?-aminoisobutyric acid (Aib, U) and named it MAP(Aib). In the MAP(Aib) molecule, five Aib residues are aligned on the hydrophobic face of the helix and five lysine (K) residues are aligned on the hydrophilic face. MAP(Aib) showed potent resistance to trypsin and pronase compared with MAP, an amphipathic helix peptide formed by usual amino acids. Fluorescein-labeled MAP(Aib) efficiently traversed the A549 cell membrane, diffusing into the cytoplasm and ...

Science.gov (United States)

Autoreactive cytotoxic CD8 T-cells (CTLs) play a key pathogenic role in the destruction of insulin-producing beta-cells resulting in type 1 diabetes. However, knowledge regarding their targets is limited, restricting the ability to monitor the course of the disease and immune interventions. In a multi-step discovery process to identify novel CTL epitopes in human preproinsulin (PPI), PPI was digested with purified human proteasomes, and resulting COOH-fragments aligned with algorithm-predicted HLA-binding peptides to yield nine potential HLA-A1, -A2, -A3 or -B7-restricted candidates. An UV-exchange method allowed the generation of a repertoire of multimers including low-affinity HLA-binding peptides. These were labeled with quantum dot-fluorochromes and encoded in a combinatorial fashion, allowing parallel and sensitive detection of specific, low-avidity T-cells. Significantly increased frequencies of ...

J-STORE (Japan)

Full Text Available

Energy Technology Data Exchange (ETDEWEB)

This report describes progress during the first year of a three-year project. The objective of the research is to examine new design approaches for achieving very high conversion efficiencies. The program is divided into two areas. The first centers on exploring new thin-film approaches specifically designed for III-V semiconductors. The second area centers on exploring design approaches for achieving high conversion efficiencies without requiring extremely high quality material. Research activities consisted of an experimental study of minority carrier recombination in n-type, metal-organic chemical vapor deposition (MOCVD)-deposited GaAs, an assessment of the minority carrier lifetimes in n-GaAs grown by molecular beam epitaxy, and developing a high-efficiency cell fabrication process.

CERN Document Server

We have developed a simulation code with the techniques which enhance both spatial and time resolution of the PM method for which the spatial resolution is restricted by the spacing of structured mesh. The adaptive mesh refinement (AMR) technique subdivides the cells which satisfy the refinement criterion recursively. The hierarchical meshes are maintained by the special data structure and are modified in accordance with the change of particle distribution. In general, as the resolution of the simulation increases, its time step must be shortened and more computational time is required to complete the simulation. Since the AMR enhances the spatial resolution locally, we reduce the time step locally also, instead of shortening it globally. For this purpose we used a technique of hierarchical time steps (HTS) which changes the time step, from particle to particle, depending on the size of the cell in which particles reside. ...

Energy Technology Data Exchange (ETDEWEB)

The development of 'Green Chemistry' requires new materials to replace the conventional organic chemistry by biological catalysts, to produce fine chemicals in an environmentally friendly manner. Microbial whole cells can be directly used as biocatalysts, providing a simple and cheap methodology since enzyme isolation and purification are avoided. High-density polyethylene (HDPE) is a very stable polymer though it can be activated by gamma radiation to induce grafting. Glycidyl methacrylate was grafted onto macroporous HDPE and PP in the range of 1-6%, proportional to the initial monomer concentration. Grafted polymers were further chemically modified with ethylenediamine to generate a cationic hydrogel of micron-size thickness onto the internal polymer surfaces. Modified polymers were able to immobilize Gram-positive and Gram-negative bacteria that can catalyze a chemical reaction as efficient as free ...

Energy Technology Data Exchange (ETDEWEB)

Discovery of better biomarkers for diagnosis, prognosis, and therapy-response prediction is the most critical task of a scientific quest aimed at developing newly designed, tailor-made therapies for patients with cancer. Consequently, a proteome wide analysis, in addition to genomic studies, is an absolute requirement for a complete functional understanding of tumor biology. Ultra-sensitive, high-performance Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) currently holds an important role in fulfilling the demands of biomarker discovery. In this review, we describe the applicability of FTICR MS for breast cancer proteomics, particularly for the analysis of complex protein mixtures obtained from a limited number of cells typically available from clinical specimens.

Energy Technology Data Exchange (ETDEWEB)

Unmanned Aerial Vehicles (UAV) are being proposed for many applications including surveillance, mapping and atmospheric studies. These applications require a lightweight, low speed, medium to long duration airplane. Due to the weight, speed, and altitude constraints imposed on such aircraft, solar array generated electric power is a viable alternative to air-breathing engines. Development of such aircraft is currently being funded under the Environmental Research Aircraft and Sensor Technology (ERAST) program. NASA Lewis Research Center (LeRC) is currently building a Solar Electric Airplane to demonstrate UAV technology. This aircraft utilizes high efficiency Applied Solar Energy Corporation (ASEC) GaAs/Ge space solar cells. The cells have been provided by the Air Force through the ManTech Office. Expected completion of the plane is early 1995, with the airplane currently undergoing flight testing using battery power.

British Library Electronic Table of Contents (United Kingdom)

Classical methods for the study of complex fluid phase behavior include static and dynamic equilibrium cells that usually require vapor and liquid recirculation. These are sophisticated, costly apparatus that require highly trained operators, usually months of labor-intensive work per mixture, and the data analysis is also rather complex. Simpler approaches to the fundamental study of azeotropes are highly desirable, even if they provide only selected cuts through the phase diagram. Recently, we introduced an advanced distillation curve measurement method featuring: (1) a composition explicit data channel for each distillate fraction (for both qualitative and quantitative analysis), (2) temperature measurements that are true thermodynamic state points that can be modeled with an equation o...

Energy Technology Data Exchange (ETDEWEB)

This inspection plan is written to meet the requirements of WAC 173-303 for operations of a TSD facility. Owners/operators of TSD facilities are required to inspection their facility and active waste management units to prevent and/or detect malfunctions, discharges and other conditions potentially hazardous to human health and the environment. A written plan detailing these inspection efforts must be maintained at the facility in accordance with Washington Administrative Code (WAC), Chapter 173-303, ``Dangerous Waste Regulations`` (WAC 173-303), a written inspection plan is required for the operation of a treatment, storage and disposal (TSD) facility and individual TSD units. B Plant is a permitted TSD facility currently operating under interim status with an approved Part A Permit. Various operational systems and locations within or under the control of B Plant have been permitted for waste management activities. ...

Energy Technology Data Exchange (ETDEWEB)

This report aims to address the potential for solid polymer fuel cell (SPFC) systems to be successfully exploited in the combined heat and power (CHP) market. A number of market studies have been undertaken to look at the size and future potential for this CHP market as well as such issues as market forces and geographical locations. This study focuses on a different aspect, that of the technical and commercial requirement together with the development needs. In order to understand how an SPFC system will have to perform, it is first necessary to examine the criteria used to judge the commercial merits of a particular CHP scheme. Issues that must be addressed include: the investment criteria - time to pay back the investment or rate of return on the investment; the influence of external factors such as electricity cost tariffs, fuel price and regulatory requirements; the cost and performance of existing technology; and the ...

Science.gov (United States)

With the advent of subgenomic hepatitis C virus (HCV) replicons, studies of the intracellular steps of the viral replication cycle became possible. These RNAs are capable of self-amplification in cultured human hepatoma cells, but save for the genotype 2a isolate JFH-1, efficient replication of these HCV RNAs requires replication enhancing mutations (REMs), previously also called cell culture adaptive mutations. These mutations cluster primarily in the central region of non-structural protein 5A (NS5A), but may also reside in the NS3 helicase domain or at a distinct position in NS4B. Most efficient replication has been achieved by combining REMs residing in NS3 with distinct REMs located in NS4B or NS5A. However, in spite of efficient replication of HCV genomes containing such mutations, they do not support production of infectious virus particles. By using the genotype 1b isolate Con1, in this study we show that REMs ...

International Nuclear Information System (INIS)

Optimal design of the hyperfractionated radiotherapy requires basic radiobiological data such as the critical dose per fraction, number of fractions per day and total equivalent dose, to name a few. As a prelude to in vivo hyperfractionated irradiation, the authors carried out experiments to determine quantitative changes in the proliferation and cell kinetic parameters of multicellular spheroids after hyperfractionated irradiation. Experiments were carried out with HeLa S-3 spheroid growing in MEM culture media. Hyperfractionated irradiation schedules were 1.5 Gy/f, 2f/day and 1.0 Gy/f, 3 f/day. At intervals after irradiation, cell numbers, growth delays and cell cycle distribution of spheroids were determined. The kinetic data were obtained by the use of flow cytometry. The most pronounced changes in cell kinetic parameters were early G/sub 2/M block, proportional to single ...

Energy Technology Data Exchange (ETDEWEB)

Biosorption is presented as an alternative choice to traditional physicochemical means for removing toxic metals from groundwater and wastewaters. Removal of lead (Pb) from solutions was studied using Escherichia coli (parental) and Vitreoscilla hemoglobin (VHb)-expressing E. coli (transformed) cells. Pb biosorption was increased in bacterial hemoglobin-expressing E. coli cells grown in Luria broth B containing different concentrations of Pb{sup 2+}. The maximum Pb{sup 2+} biosorption of transformed and parental cells was determined to be 612 and 370 {mu}g Pb/g biomass, respectively. The inhibitory effect of Pb{sup 2+} on the parental strain was determined at 10 ppm. However, in transformed cells, Pb{sup 2+} was lethal at 100 ppm. The optimum aeration required for the transformed cells was lower than that for the parental strain on a growth yield basis. A linear ...

Energy Technology Data Exchange (ETDEWEB)

The rechargeability of the slurry-Zn/air battery was demonstrated with a practical recharge cell that requires minimal hydraulic and mechanical energy for operation. A dendritic Zn was deposited on a Mg plate substrate from which it was easily, periodically and automatically scraped to regenerate dendritic Zn slurries. Excellent discharge results were obtained with the regenerated dendritic Zn slurry, comparable to those obtained with slurries made with mixtures of Zn powder. The dendritic Zn slurry allowed, however, twice the utilization of Zn. 13 refs., 24 figs., 2 tabs.

CERN Document Server

We propose a mechanism to explain what occurs when a mixture of grains of different sizes and different shapes (i.e. different repose angles) is poured into a quasi-two-dimensional cell. Specifically, we develop a model that displays spontaneous stratification of the large and small grains in alternating layers. We find that the key requirement for stratification is a difference in the repose angles of the two pure species, a prediction confirmed by experimental findings. We also identify a kink mechanism that appears to describe essential aspects of the dynamics of stratification.

Energy Technology Data Exchange (ETDEWEB)

This document reports the progress and accomplishments of a 16 month program to develop a rechargeable zinc-air battery for electric vehicle propulsion, from October 1988 through January 1990. The program was the first stage in the transition of alkaline zinc electrode technology, invented at Lawrence Berkeley Laboratory, to private industry. The LBL invention teaches the use of a copper metal foam substrate for the zinc electrode, in combination with forced convection of electrolyte through the foam during battery operation. Research at LBL showed promise that this approach would avoid shape change (densification and dendrite growth), the primary failure mode of this electrode. The program comprised five tasks; (1) cell design, (2) capacity maximization, (3) cycle testing, (4) materials qualification, and (5) a cost/design study. The cell design contemplates a plate and frame stack, with alternating zinc and oxygen electrode frame assemblies ...

CERN Document Server

In this paper, we describe our vectorized and parallelized adaptive mesh refinement (AMR) N-body code with shared time steps, and report its performance on a Fujitsu VPP5000 vector-parallel supercomputer. Our AMR N-body code puts hierarchical meshes recursively where higher resolution is required and the time step of all particles are the same. The parts which are the most difficult to vectorize are loops that access the mesh data and particle data. We vectorized such parts by changing the loop structure, so that the innermost loop steps through the cells instead of the particles in each cell, in other words, by changing the loop order from the depth-first order to the breadth-first order. Mass assignment is also vectorizable using this loop order exchange and splitting the loop into $2^{N_{dim}}$ loops, if the cloud-in-cell scheme is adopted. Here, $N_{dim}$ is the number of dimension. These ...

Energy Technology Data Exchange (ETDEWEB)

Using a coplanar, end-mounted antenna as a baseline, various configuration trades were performed to select a preferred solid state concept. The increase in efficiency that could be realized by use of multi bandgap solar cells, either with klystron or solid state antenna was evaluated. Satellite configurations were developed to exploit the sandwich antenna concept wherein solar cells are located on one side of the antenna panel and solid state dc/RF converters on the other side. These concepts entailed various primary and secondary reflector arrangements for directing solar energy to the solar cell side of the antenna with higher concentration ratios than used on the coplanar configurations. Operations analysis included development of a satellite construction scenario, a concept for the SCB, a top-level satellite construction operation, construction operation, construction timelines and crew sizes, mass flows to orbit, and a ...

International Nuclear Information System (INIS)

This paper demonstrates that a commercial-scale facility for encapsulating krypton-85 in zeolite-5A or glass at a 2000 MTHM per year nuclear fuel reprocessing plant can be designed to contain fragments and the 340 to 850 kCi krypton-85 inventory from an assumed catastrophic failure of the high pressure vessel. The vessel failure was assumed as a worst case and was not based on a detailed design evaluation or operating experience. The process design is based on existing commercial hot isostatic pressing technology operated at up to 40 times the scale required for krypton encapsulation. From the calculated process gas inventory in the pressure vessel and vessel design, the maximum explosive energy of 8.4 kg TNT and resulting vessel plug and fragment velocities were calculated. The facility Containment Cell housing the high pressure vessel was designed to contain the gases, fragments, and the shock wave energy calculated for a hypothetical vessel ...

International Nuclear Information System (INIS)

In the High Flux Reactor (HFR) at Grenoble a new horizontally arranged cold neutron source will be installed that uses liquid deuterium (D_2) as the moderator for cold neutrons. This cold source should provide a high neutron flux, it should be simple in design, and be characterized by high reliability and by safe operation. A high neutron flux calls for installation of the cold source near the HFR core and good moderation requires a D_2 volume of #DELTA#5 litres. Hence, the moderator, contained in a horizontally arranged cylindrical cell of 21 cm diameter and 20 cm length, is installed at the end nearest to the core of a horizontal beam tube of roughly 4.5 m length with an inner diameter of only 23 cm (Fig. 1). The HFR will be equipped with a second cold neutron source. The installation in the existing horizontal beam tube together with the amount of heat released determined the problems to be solved: the liquid content of the moderator ...

Energy Technology Data Exchange (ETDEWEB)

The aim of this contract is to investigate, develop, and characterize laser-assisted processing techniques utilized to produce the fine-line, thin-metal grid structures that are required to fabricate high-efficiency solar cells. During the first quarter of this contract, a comprehensive literature search was carried out in the various state-of-the-art laser-assisted techniques for metal deposition, including laser chemical vapor deposition and laser photolysis of organometallics, as well as laser-enhanced electroplating. A compact system for the experiments involving laser-assisted photolysis of gas-phase compounds was designed and constructed. The work performed in the second quarter is detailed in this report. Metal deposition experiments have been carried out utilizing laser-assisted pyrolysis of a variety of metal-bearing polymer films and metallo-organic inks spun onto silicon substrates. Laser decomposition of spun-on silver neodecanoate ...

Energy Technology Data Exchange (ETDEWEB)

In our previous work, we demonstrated that biomass-generated producer gas can be converted to ethanol and acetic acid using a microbial catalyst Clostridium carboxidivorans P7{sup T}. Results showed that the producer gas (1) induced cell dormancy, (2) inhibited H{sub 2} consumption, and (3) affected the acetic acid/ethanol product distribution. Results of this work showed that tars were the likely cause of cell dormancy and product redistribution and that the addition of a 0.025{mu}m filter in the gas cleanup negated the effects of tars. C. carboxidivorans P7{sup T} can adapt to the tars (i.e. grow) only after prolonged exposure. Nitric oxide, present in the producer gas at 150ppm, is an inhibitor of the hydrogenase enzyme involved in H{sub 2} consumption. We conclude that significant conditioning of the producer gas will be required for the successful coupling of biomass-generated producer gas with fermentation to produce ...

Energy Technology Data Exchange (ETDEWEB)

A p[sup +]-Al[sub 0.3]Ga[sub 0.7]As/n[sup +]-GaAs heterojunction tunnel diode was fabricated using Atomic Layer Epitaxy (ALE) growth technique. Background carbon doping of [similar to]10[sup 20] cm[sup [minus]3] was achieved in the p-side of the diode by optimizing growth conditions such as V/III ratio, exposure times to reactant gases, and growth temperature. In the n-side of the diode GaAs was doped with silane and doping concentrations as high as 7[times]10[sup 18] cm[sup [minus]3] were also achieved. The dopants are chosen to satisfy the high levels and low diffusion requirements. The diode can be used to interconnect the high and low band-gap cells in the AlGaAs/GaAs cascade solar cell structure. The reactor used in this investigation is a commercial MOCVD system which has been specially modified for dual operation of ALE and MOCVD growth modes.

Science.gov (United States)

Thin film solar cells and solar cell testing - photovoltaic cells, radiation damage to cadmium sulfide solar cells, and airplane testing of solar cells

Energy Technology Data Exchange (ETDEWEB)

Purified protein kinase C (C-kinase) from guinea pig pancreas and rat brain stimulated adenylate cyclase activity in purified rat adipocyte membranes. Cyclase stimulation occurred over 100 to 1000 mU/ml of C-kinase activity, required greater than 10 ..mu..M calcium, proceeded without a lag, was not readily reversible, and required no exogenous phospholipid. Moreover, C-kinase inhibitors, such as chlorpromazine and palmitoyl carnitine, inhibited selectively adenylate cyclase which was activated by C-kinase and calcium. Depending on assay conditions, 10 nM 12-0-tetradecanoylphorbol-13-acetate (TPA) either enhanced or was required for kinase action on cyclase. Also, TPA plus calcium promoted the quantitative association of C-kinase with membranes. Adenylate cyclase activation by C-kinase was seen both in the presence and absence of exogenous GTP, indicating that the kinase effect does not result from an action on the ...

Energy Technology Data Exchange (ETDEWEB)

This document describes the development testing phase associated with the OTA Shear activity and defines the controls to be in place throughout the testing. The purpose of the OTA Shear Program was to provide equipment that is needed for the processing of 40 foot long, sodium wetted, irradiated core components previously used in the FFTF reactor to monitor fuel and materials tests. There are currently 15 of these OTA test stalks located in the Test Assembly Conditioning Station (TACS) inerted vault. These need to be dispositioned for a shutdown mission to eliminate this highly activated, high dose inventory prior to turnover to the ERC since they must be handled by remote operations. These would also need to be dispositioned for a restart mission to free up the vault they currently reside in. The waste handling and cleaning equipment in the J33M Cell was designed and built for the handling of reactor components up to the standard 12 foot length. This program will ...

International Nuclear Information System (INIS)

Full text: DNA rearrangement events leading to chromosomal aberrations are central to ionizing radiation-induced cell death. Although DNA double-strand breaks are probably the lesion that initiates formation of chromosomal aberrations, little is understood about the molecular mechanisms that generate and modulate DNA rearrangement. Examination of the sequences that flank sites of DNA rearrangement may provide information regarding the processes and enzymes involved in rearrangement events. Accordingly, we developed a method using inverse PCR that allows the detection and sequencing of putative radiation-induced DNA rearrangements in defined regions of the human genome. The method can detect single copies of a rearrangement event that has occurred in a particular region of the genome and, therefore, DNA rearrangement detection does not require survival and continued multiplication of the affected cell. Ionizing ...

Energy Technology Data Exchange (ETDEWEB)

A new type of zinc electrode is matched with new bifunctional air electrodes to produce a zinc-air battery of a novel design. The zinc electrode is a flow-thru type made from copper foam-metal. The air electrode uses corrosion resistant carbon black as a high area support for a highly dispersed spinel oxide electrocatalyst. The battery design employs flowing electrolyte, 12 M KOH saturated or supersaturated with zincate. Single cells as large as 200 cm{sup 2} (1/5 EV design scale) having a capacity of 20 AH have been tested with C/4--C/16 constant current cycling. More extensive and realistic life cycle testing was done with 2 Ah cells, including the Simplified Federal Urban Driving Schedule (SFUDS) cycle. This testing has confirmed that these cells can provide the necessary transient power response required for urban EV applications. The cells achieved an average of 72 SFUDS ...

Energy Technology Data Exchange (ETDEWEB)

Studies were conducted to examine the pressure on fluid-filled cables of electric power transmission networks in the event of an oil leak. Transition splices between paper-insulated oil-impregnated cable systems (PILC) and solid dielectric cable is difficult to perform. A proper PILC is composed of a means to contain oil within the cable structure and to keep moisture out. A failure of the cable joint could occur if an oil leak dries out the paper. Since oil pressure in most PILC cables is high, strong mechanical means must be devised to contain the oil within the cable. Resin systems, heat shrink systems and wiped lead sleeves to reseal the PILC cable over the splice, are among the methods used to stop an oil leak. A system has been designed using an EPDM tube contained under mechanical pressure; it has proven to be effective over a wide range of pressure conditions. Test results utilizing this system were presented. 1 ref., 1 tab., 4 figs.

Energy Technology Data Exchange (ETDEWEB)

Simultaneously extracted metals (SEM) and acid volatile sulfide (AVS) show the potential for toxicity on the basis of their ratio. Accordingly, the authors spiked cadmium in a range for which Cd/AVS ratios were from 0.2 to 10 in the sediment with its weight about 8 kg in each batch. Dialysis samplers with a cation ion-exchange resin (Dowex 50W-X4) collection were used in a laboratory for the determination of free cadmium concentrations in pore water of the collected sediment. When equilibrium was reached among cadmium in pore water, sediment, and ion-exchange resin, cadmium exchanged onto resin phase was regenerated with 1 N hydrochloric acid (OPTIMA grade) and determined using an atomic absorption spectrophotometer (Zeeman 5000) with a graphite furnace accessory. Cadmium determined using the dialysis sampler is considered as free cadmium which is related to the metal bioavailability toward aquatic biota. The developed methodology provides a new technique for ...

International Nuclear Information System (INIS)

Growing public and political interests towards incorporating passive safety features in nuclear installations, let Siempelkamp in late 1987 propose a solution consisting of a prestressed cast-iron pressure vessel and a passive heat removal system, integrated in the reactor cell surrounding the vessel. This solution combines the inherent safety of a prestressed metallic pressure vessel with the advantages of a passive heat removal system and thus constitutes a major step towards the goal of further reducing potential residual risks. The design had to meet the boundary conditions for reactor core and reactor building of the modular 200 MWth pebble bed reactor of Siemens/-KWU. The engineering design showed that many input parameters needed for the finite-element-analysis of the overall structure required a verification by measurements in a well scaled test setup. This was especially required for the heat transfer from the ...

International Nuclear Information System (INIS)

The first essential before embarking on the radiation-hardening of electrical insulation - mostly magnet coils - in any beam line application is to obtain a reliable estimate of the dose to the components. These are examples ( switchyards at SLAC and LAMPF) where the degree of hardness specified was much higher than was required. Although experience shows that the cost premium for substantial radiation - hardening is of the order of 10%, it has also become clear that well - designed beam line have negligible losses: hardening is required only in the vicinity of targets, collimators or other beam - intercepting devices. Where the beam is deliberately scraped, local shielding will minimize the associated radiation in the surroundings. Electron machines have their own special problems due to synchrotron radiation, so certainly coils and other electrical equipment should be kept away from the beam bend - plane. Because proton beams interact with ...

DEFF Research Database (Denmark)

Udgivelsesdato: 2002-Mar

Science.gov (United States)

PMID:21290990

International Nuclear Information System (INIS)

Interleukin 2 (IL-2) is a T-cell-derived lymphokine critical in the activation and proliferation of T cells, B cells, and lymphokine-activated killer cells. It is a glycoprotein of #approx#15,500 daltons that is synthesized and secreted after activation by antigen or mitogen. By using the analogs 8-azidoadensoine 5'-[#gamma#-"3"2P]triphosphate ([#gamma#-"3"2P]8N_3ATP) and nicotinamide 2-azidoadenine [adenylate-"3"2P]dinucleotide ([#alpha#-"3"2P]2N_3NAD"+) as photoaffinity probes, the authors have detected specific, metal ion-requiring nucleotide binding sites on recombinant human IL-2 (rhIL-2). The specificity of these nucleotide interactions with rhIL-2 was demonstrated by saturation effects and by competition by the parent nucleotides at physiologically relevant concentrations. Saturation of photoinsertion into rhIL-2 occurred at 50 #mu#M [#gamma#-"3"2P]8N_3ATP. Saturation of ...

International Nuclear Information System (INIS)

Asialoorosomucoid (ASOR) is internalized and degraded by HepG2 cells after binding to the asialoglycoprotein (ASGP) receptor, internalization through the coated pit/coated vesicle pathway, and trafficking to lysosomes. Primaquine, an 8-aminoquinoline antimalarial compound, inhibits ASOR degradation at concentrations greater than 0.2 mM by neutralizing intracellular acid compartments. This leads to alterations in surface receptor number, receptor-ligand dissociation, and receptor recycling. We have investigated the effects of primaquine on 125I-ASOR uptake and degradation as a function of primaquine concentration and duration of exposure. Concentrations below those required for neutralization of acidic compartments block 125I-ASOR degradation in HepG2 cells and lead to intracellular ligand accumulation. This effect is maximal at 80 microM primaquine. The intracellular 125I-ASOR is undegraded, dissociated from the ASGP ...

Energy Technology Data Exchange (ETDEWEB)

Two-phase flow in horizontal parallel channels has been experimentally investigated under fuel cell related operating conditions. Pronounced hysteresis is observed in the pressure drop versus flow characteristic curve when starting from either flooded or dry conditions. When gas is introduced into channels initially filled with water (flooded initial condition), both gas and liquid tend to flow predominantly in one channel at low gas or liquid flow velocities. As the gas flow velocity increases, even distribution of gas and liquid flow in both channels is observed, accompanied with a sudden decrease in the pressure drop. On the other hand, even gas and liquid flow distribution between both channels is found at comparatively lower gas flow velocities when starting with dry-gas flow conditions with liquid introduced into channels filled with gas (stratified flow regime). The flow regimes of this system are visualized in plots of the pressure drop against gas and ...

Science.gov (United States)

Cytosine methylation in DNA is a major epigenetic signal, and plays a central role in propagating chromatin status during cell division. However the mechanistic links between DNA methylation and histone methylation are poorly understood. A multi-domain protein UHRF1 (ubiquitin-like, containing PHD and RING finger domains 1) is required for DNA CpG maintenance methylation at replication forks, and mouse UHRF1-null cells show enhanced susceptibility to DNA replication arrest and DNA damaging agents. Recent data demonstrated that the SET and RING associated (SRA) domain of UHRF1 binds hemimethylated CpG and flips 5-methylcytosine out of the DNA helix, whereas its tandom tudor domain and PHD domain bind the tail of histone H3 in a highly methylation sensitive manner. We hypothesize that UHRF1 brings the two components (histones and DNA) carrying appropriate markers (on the tails of H3 and hemimethylated CpG sites) ready to be ...

Energy Technology Data Exchange (ETDEWEB)

A lightweight, low permeability liner for graphite epoxy composite compressed gas storage vessels. The liner is composed of polymers that may or may not be coated with a thin layer of a low permeability material, such as silver, gold, or aluminum, deposited on a thin polymeric layer or substrate which is formed into a closed bladder using torispherical or near torispherical end caps, with or without bosses therein, about which a high strength to weight material, such as graphite epoxy composite shell, is formed to withstand the storage pressure forces. The polymeric substrate may be laminated on one or both sides with additional layers of polymeric film. The liner may be formed to a desired configuration using a dissolvable mandrel or by inflation techniques and the edges of the film seamed by heat sealing. The liner may be utilized in most any type of gas storage system, and is particularly applicable for hydrogen, gas mixtures, and oxygen used for vehicles, fuel ...

International Nuclear Information System (INIS)

Papers are presented on space power requirements and issues, space photovoltaic systems, space solar dynamic systems, space thermal systems, manned and unmanned space power systems, thermionics, and thermoelectrics. Also considered are high power devices for space power systems, high power conversion for space power systems, 1-10 kWe nuclear space power sources, 100-kW class nuclear power concepts, space reactor safety, and multimegawatt space nuclear power systems. Other topics include space power systems automation, space kilovolt technology, space power electronics, space lithium and nickel-cadmium batteries, lithium sodium storage, and space fuel cells. Papers are also presented on space nickel hydrogen batteries, alternative energy concepts and fuels, fuel cell technology, flow batteries, high-temperature batteries, energy conservation, battery energy storage, thermal energy storage, heat engines, MHD power systems, ...

International Nuclear Information System (INIS)

The present study sought a biochemical explanation for retarded brain development in the heterozygous offspring of the phenylketonuric (PKU) mother. Two rat models of simulated maternal PKU, one induced by p-chloropheylalanine and phenylalanine and the other by phenylacetate, were employed in this investigation. Maternal PKU had no influence on cerebral concentrations of DNA, protein, and cholesterol, which were normal in the 2 d old pup. However, there was a noticeable disruption of the normal ganglioside pattern and a significant reduction of sialoglycoproteins. Concomitant with a delayed drop in the gangliosides Q/sub 1b/ and D_3, was a slower rise in M_1 and D/sub 1a/. At least 66% of sialoglycoproteins located on SDS-PAGE gel chromatograms, by radioactivity incorporated in vivo from radiolabeled N-acetylmannosamine and by ("3H) sialic acid released by neuraminidase from periodate-("3H) borohydride labeled glycoproteins, have mobilites of the cell adhesion ...

Energy Technology Data Exchange (ETDEWEB)

The numerous challenges facing the North American electric utility industry were discussed. In addition to competition, electric utilities are faced with low demand growth, public pressure to avoid adding transmission and distribution capacity, and strict environmental requirements. In view of these developments, large central generation additions that have been industry norm until recently, do not always make economic sense in the current environment. Instead, smaller, distributed additions to generating capacity is becoming the new norm. Small-scale on-site generating capacity has the added advantage of reducing the need for expensive high-voltage transmission lines. The Ballard generation system described here is a modular, efficient fuel cell-based solution to implementing distributed power concepts. The system is natural gas fueled, with low noise, vibration and emissions. Apart from its other advantages, it provides ideal convergence ...

British Library Electronic Table of Contents (United Kingdom)

Short synthetic single-stranded oligodeoxyribonucleotides (ssODNs) can be used to introduce subtle modifications into the genome of mouse embryonic stem cells (ESCs). We have previously shown that effective application of ssODN-mediated gene targeting in ESC requires (transient) suppression of DNA mismatch repair (MMR). However, whereas transient down-regulation of the mismatch recognition protein MSH2 allowed substitution of 3 or 4 nucleotides, 1 or 2 nucleotide substitutions were still suppressed. We now demonstrate that single- or dinucleotide substitution can effectively be achieved by transient down-regulation of the downstream MMR protein MLH1. By exploiting highly specific real-time PCR, we demonstrate the feasibility of substituting a single basepair in a non-selectable gene. Howev...

Energy Technology Data Exchange (ETDEWEB)

Long before possible organic effects, exposure to ionizing radiations can provoke anxiety. In front of invisibility, the imagination quickly ignites. The terrorists have perfectly understood it. They are ready to use ionizing radiations as a weapon to remind traumatic images deeply rooted in people's memory. These images induce anxiety with all the clinical expressions connected to it. These symptoms require to be treated because of a possible anarchic development. For that purpose, plans have been elaborated to coordinate the different professional's actions. The coherence of medical management and communication aims to allow the most implicated people to find the way to face the events. When it is not possible, medico-psychological cells permit a specialized care. (author)

Energy Technology Data Exchange (ETDEWEB)

Electric Fuel Ltd. is engaged in the design, development and commercialization of its proprietary zinc/air battery technology for electric vehicles, consumer electronic products and defence applications. To meet the challenging requirements for propelling an all-electric bus, the Vehicle Division sought a unique solution: an all electric battery-battery hybrid propulsion system. The high energy zinc/air battery is coupled with a high-power auxiliary battery. The combined system offers zero emission, high power and long range in an economically viable package. The consumer battery group has developed a high power primary zinc/air cell aimed at cellular phone users, offering extended use, convenience and low cost. (orig.)

Science.gov (United States)

The glass gene is required for proper photo-receptor differentiation during development of the Drosophila eye glass codes for a DNA-binding protein containing five zinc fingers that we show is a transcriptional activator. A comparison of the sequences of the glass genes from two species of Drosophila and a detailed functional domain analysis of the Drosophila melanogaster glass gene reveal that both the DNA-binding domain and the transcriptional-activation domain are highly conserved between the two species. Analysis of the DNA-binding domain of glass indicates that the three carboxyl-terminal zinc fingers alone are necessary and sufficient for DNA binding. We also show that a deletion mutant of glass containing only the DNA-binding domain can behave in a dominant-negative manner both in vivo and in a cell culture assay that measures transcriptional activation. PMID:7604032

Science.gov (United States)

We developed mice with germline endogenous expression of oncogenic Hras to study effects on development and mechanisms of tumor initiation. They had high perinatal mortality, abnormal cranial dimensions, defective dental ameloblasts, and nasal septal deviation, consistent with some of the features of human Costello syndrome. These mice developed papillomas and angiosarcomas, which were associated with Hras(G12V) allelic imbalance and augmented Hras signaling. Endogenous expression of Hras(G12V) was also associated with a higher mutation rate in vivo. Tumor initiation by Hras(G12V) likely requires augmentation of signal output, which in papillomas and angiosarcomas is achieved via increased Hras-gene copy number, which may be favored by a higher mutation frequency in cells expressing the oncoprotein. PMID:19416908

British Library Electronic Table of Contents (United Kingdom)

Amino-rich polymeric coatings are widely used in biomedical applications, since they promote adsorption of diverse biomolecules or facilitate cell growth. As a consequence, there is a growing interest in fabrication of such coatings that is focused predominantly on the optimization of the deposition process in terms of high density of primary amino groups. In addition, the nature of biomedical applications requires also sufficient stability of the films in aqueous environments. This aspect is investigated in this contribution. In particular, the effect of water and phosphate buffer saline on the coatings prepared by RF magnetron sputtering of Nylon 6,6 in Ar/N2 and N2/H2 gas mixtures is evaluated. The samples exposed to liquids are characterized by various diagnostic methods and their prop...

Science.gov (United States)

Course website from Cambridge on ceramic materials. This site contains eight lectures in PDF format (Adobe Reader required), two question sheets, five practicals (including answers), digital movies and further useful links. "There is a strong relationship between the structure of a material and its physical properties. The properties of a material whether mechanical, electrical, optical or magnetic, determine how it can be used in practical applications. In this course, the focus is on the structure / property relationship for ionic materials with electrical properties utilised in various transducer devices. The materials considered include perovskites, which have special polarisation properties exploited in ferroelectric, pyroelectric and piezoelectric devices. Other oxides, such as zirconia, have structures permitting rapid diffusion of ions, making them suitable for use in sensors, fuel cells and batteries. The scientific principles ...

British Library Electronic Table of Contents (United Kingdom)

Occupation of the coast has significantly increased in recent decades, mostly due to a greater demand for recreation and tourism. Today, erosion threatens many human-made structures and activities, requiring an integrated approach for the understanding of coastal dynamics and identification of alternatives to associated problems. This study investigates a 64km-long coastal physiographic unit in the northern microtidal littoral of Tuscany (Italy). Vertical aerial photographs and direct field surveys were used to retrieve changes in shoreline position over 1938-1997 and 1997-2005 time intervals. Significant beach accretion was observed during the first period updrift of Carrara (84m) and Viareggio (280m) harbours and at Marina di Pietrasanta (100m), whereas severe erosion occurred downcoast ...

Energy Technology Data Exchange (ETDEWEB)

The use of deuterium-tritium fuel in the Compact Ignition Tokamak will require applying remote handling technology for ex-vessel maintenance and replacement of machine components. Highly activated and contaminated components of the fusion devices auxiliary systems, such as diagnostics and RF heating, must be replaced using remotely operated maintenance equipment in the test cell. In-vessel remote maintenance included replacement of divertor and first wall hardware, faraday shields, and for an in-vessel inspection system. Provision for remote replacement of a vacuum vessel sector, toroidal field coil or poloidal field ring coil was not included in the project baseline. As a result of recent coil failures experienced at a number of facilities, the CIT project decided to reconsider the question of remote recovery from a coil failure and, in January of 1990, initiated a coil replacement study. This study focused on the technical ...

CERN Document Server

We re-examine the process of loop quantization for flat isotropic models in cosmology. In particular, we contrast different inequivalent `loop quantizations' of these simple models through their respective successes and limitations and assess whether they can lead to any viable physical description. We propose three simple requirements which any such admissible quantum model should satisfy: i) independence from any auxiliary structure, such as a fiducial interval/cell introduced to define the phase space when integrating over non-compact manifolds; ii) existence of a well defined classical limit and iii) provide a sensible "Planck scale" where quantum gravitational effects become manifest. We show that even when it may seem that one can have several possible loop quantizations, these physical requirements considerably narrow down the consistent choices. Apart for the so called improved dynamics of LQC, none of the other ...

International Nuclear Information System (INIS)

Thermophotovoltaic (TPV) systems are attractive possibilities for direct thermal-to-electric energy conversion, but have typically required the use of black body radiators operating at high temperatures. Recent advances in both the understanding and performance of solid rare-earth oxide selective emitters make possible the use of TPV at temperatures as low as 1200K. Both selective emitter and filter system TPV systems are feasible. However, requirements on the filter system are severe in order to attain high efficiency. A thin-film of a rare-earth oxide is one method for producing an efficient, rugged selective emitter. An efficiency of 0.14 and power density of 9.2 W/KG at 1200K is calculated for a hypothetical thin-film neodymia (Nd2O3) selective emitter TPV system that uses radioisotope decay as the thermal energy source.

Energy Technology Data Exchange (ETDEWEB)

Abstract not provided

International Nuclear Information System (INIS)

... electric utilities electrochemistry energy storage fuel cells organizational models

UK PubMed Central (United Kingdom)

We studied telomeric DNA in leukemic cells as well as in normal T cells, B cells, monocytes, polymorphonuclear leukocytes, and bone marrow hematopoietic progenitor cells. No marked differences were...Full Text Available

International Nuclear Information System (INIS)

The detection and quantification of defined reaction products of chemical mutagens and carcinogens (and of many cancer chemotherapeutic agents) with DNA require highly sensitive analytical techniques. The exceptional capability of immunoglobulins to recognize subtle alterations of molecular structure (especially when monoclonal antibodies are used to maximize specificity), outstanding sensitivity of immunoanalysis by high-affinity antibodies, and the fact that radioactively-labelled agents are not required suggest the utility of a radioimmunoassay to recognize and quantitate alkylated DNA products. We have recently developed a set of high-affinity monoclonal antibodies (secreted by mouse x mouse as well as by rat x rat hybridomas; antibody affinity constants, 10"9 to > 10"1"0 lmol) specifically directed against several DNA alkylation products with possible relevance in relation to both mutagenesis and malignant transformation of mammalian ...

Energy Technology Data Exchange (ETDEWEB)

The Westinghouse Electric Corporation has undertaken to investigate, develop, and characterize laser-assisted processing techniques utilized to produce the fine line, thin metal grid structures that are required to fabricate high-efficiency solar cells. Two basic techniques for metal deposition will be investigated, as follows: (1) photochemical decomposition of liquid or gas phase organometallic compounds utilizing either a focused, CW ultraviolet laser (System 1) or a mask and ultraviolet flood illumination, such as that provided by a repetitively pulsed, defocused excimer laser (System 2), for pattern definition, and (2) thermal deposition of metals from organometallic solutions or vapors utilizing a focused, CW laser beam as a local heat source to draw the metallization pattern. The purpose of this contract is to investigate the various existing laser-assisted film deposition techniques in order to develop a new, cost-effective technology ...

Energy Technology Data Exchange (ETDEWEB)

Successful interface engineering requires compositional and electronic material characterization as a prerequisite for understanding and intentionally generating interfaces in photovoltaic devices. The paper gives an overview with several examples, all referring to Cu(In,Ga)(S,Se){sub 2} ('CIGSSe')-based solar cells, with an emphasis on characterization using highly specialized methods, such as elastic recoil detection analysis, X-ray emission spectroscopy and photoelectron spectroscopy using synchrotron and ultraviolet light for excitation, inverse photoemission spectroscopy and Kelvin probe force microscopy. First, the determination of the depth profile of the band gap energy E{sub g} in the absorber layer is demonstrated. The modification of E{sub g} towards both interfaces is discussed in terms of beneficial electronic effects. Next, the interface between absorber and buffer layers with alternative and promising non-toxic ...

Energy Technology Data Exchange (ETDEWEB)

Background and Purpose: Total-body irradiation (TBI) is an important part of the conditioning regimen for hematopoietic stem cell transplantation (HSCT) in patients with hematologic malignancies. The results after treatment with various TBI regimes were compared, and dose-effect relationships for the endpoints relapse incidence, disease-free survival, treatment-related mortality, and overall survival were derived. The aim was to define requirements for an optimal treatment schedule with respect to leukemic cell kill and late normal-tissue morbidity. Material and Methods: A literature search was performed. Three randomized studies, four studies comparing results of two or three TBI regimens, and nine reports with results of one specific TBI regimen were identified. Biologically effective doses (BEDs) were calculated. The results of the randomized studies and the studies comparing results of two or three TBI regimens were ...

Energy Technology Data Exchange (ETDEWEB)

Because of its high energy density, compatibility with aqueous electrolytes and the low toxicity of its active materials, the zinc-air battery system is an interesting candidate for electric vehicle applications. The use of O{sub 2} from the air as a reactant requires a partially open cell construction and a technologically challenging air interface. This report describes the research and development program at the Paul Scherrer Institute which finally led to the demonstration of a durable, electrically rechargeable zinc-oxygen battery. In a first phase the research program was focused on the development of bifunctional oxygen diffusion electrodes and pasted zinc electrodes. The current-potential behaviour and the cycle life performance of anodes and cathodes was tested in single electrode measurements (three-electrode arrangements) as well as in complete monopolar zinc-oxygen and zinc-air cells. La{sub 0.6}Ca{sub ...

Science.gov (United States)

A silicon solar cell assembly comprising a large, thin silicon solar cell bonded to a metal mount for use when there exists a mismatch in the thermal expansivities of the device and the mount.

UK PubMed Central (United Kingdom)

Ethane oxidation was studied in ethane-grown resting cells (mycelia) of an Acremonium sp. and in cell-free preparations of such mycelia. From resting cell experiments evidence was found for a pathway...Full Text Available

Science.gov (United States)

... FLUENT PEM fuel cell toolbox is actually composed of many User Defined Functions that are incorporated into the fuel cell model developed by ...

DEFF Research Database (Denmark)

Udgivelsesdato: 2009-Jun

International Nuclear Information System (INIS)

Cell cultures were prepared from lung tumors occurring in beagles following exposure to inhaled plutonium. Morphologic and growth characteristics of two of these cell lines are described.

Science.gov (United States)

PMID:17103063

Science.gov (United States)

IntroductionBreast cancer is the most diagnosed and second leading cause of cancer deaths in the U.S. female population. An estimated 5 to 10 percent of all breast cancers are inherited, caused by mutations in the breast cancer susceptibility genes (BRCA1/2). As many as 90% of all mutations are nonsense mutations, causing a truncated polypeptide product. A popular and low cost method of mutation detection has been the protein truncation test (PTT), where target regions of BRCA1/2 are PCR amplified, transcribed/translated in a cell-free protein synthesis system and analyzed for truncated polypeptides by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and autoradiography. We previously reported a novel High Throughput Solid-Phase PTT (HTS-PTT) based on an enzyme-linked immunosorbent assay (ELISA) format that eliminates the need for radioactivity, SDS-PAGE and subjective interpretation of the results. Here, we report the next generation HTS-PTT ...

UK PubMed Central (United Kingdom)

Terminal differentiation of many vascular cells involves cell wall changes. Cells first elongate their primary wall, then lay down a lignified secondary wall, which is often followed by digestion of...Full Text Available

UK PubMed Central (United Kingdom)

Totipotent stem cells have the potential to differentiate into every cell type. Renewal of totipotent stem cells in the germline and cellular differentiation during early embryogenesis rely upon posttranscriptional...Full Text Available

UK PubMed Central (United Kingdom)

AIM: To isolate and identify the biological characteristics of human colon cancer stem cells (SW1116 cells) and further study their proteome.METHODS: SW1116 cells were isolated and cultured with...Full Text Available

Science.gov (United States)

Not Available

UK PubMed Central (United Kingdom)

BackgroundStem cell characteristics are an important feature of human cancer cells and play a major role in the therapy resistance of tumours. Strategies to target cancer stem cells...Full Text Available

UK PubMed Central (United Kingdom)

We describe the lethal, recessive accelerated-cell-death11 Arabidopsis mutant (acd11). Cell death in acd11 exhibits characteristics of animal apoptosis...Full Text Available

UK PubMed Central (United Kingdom)

Background:Stem cells can differentiate into multiple cell types, and therefore can be used for cellular therapies, including tissue repair. However, the participation of stem cells...Full Text Available

Science.gov (United States)

Not Available

UK PubMed Central (United Kingdom)

Hematopoietic stem cells replenish all the cells of the blood throughout the lifetime of an animal. Although thousands of stem cells reside in the bone marrow, only a few contribute to blood production...Full Text Available

KoreaScience (Korea)

Full Text Available

UK PubMed Central (United Kingdom)

In the growing chloronema cell suspension cultures of the moss Funaria hygrometrica Hedw., activities of several enzymes have been found to be cell-density-dependent. Cyclic nucleotide...Full Text Available

Science.gov (United States)

...false Guidelines for Human Stem Cell Research Presidential Documents ...2009 Guidelines for Human Stem Cell Research Memorandum for the Heads...scientifically worthy human stem cell research, including human...

Wastenet

...Basic Information | Fuel Cells & Vehicles | US EPA This web page provides basic information on EPA's Fuel Cells & Vehicles web site ...including the chemical composition of fuel cell technology, how it works, descriptions of the various types of fuel cells , their availability and ... background,electrochemical,hydrogen,fuel cell,fuel cell vehicle,fuel reformer,performace,improved fuel economy,increased engine efficiency,lower emissions,zero emissions,availablity,fuel cell types,diagram,Proton ...Exchange Membrane,PEM Basic Information | Fuel Cells & Vehicles | US EPA background,electrochemical,hydrogen,fuel cell,fuel cell vehicle,fuel reformer,performace,improved fuel economy,...

Science.gov (United States)

Students get a background on Stem Cell technology and learn to write an effective opinion essay.

Energy Technology Data Exchange (ETDEWEB)

Laser-assisted processing techniques for producing high-quality solar cell metallization patterns are investigated, developed, and characterized. The tasks comprising these investigations are outlined. A new batch of solar cells was processed using the laser decomposition of spun-on silver neodecanoate to metallize cells. Decomposition of silver neodecanoate was carried out at different laser powers on different cells on a given wafer to determine whether this would have any effect on cell performance. Solar cells which were written with laser powers of 5 to 8 watts, gave excellent results with cell efficiencies in the range of 14 to 16%.

Science.gov (United States)

... nent to the design and construction of metal-semiconductor solar cells, in that both the photovoltage and the efficiency of metal-semiconductor cells ...

Energy Technology Data Exchange (ETDEWEB)

This handbook provides the necessary tools for the engineer to design forced-cooled HPOF pipe-type cable systems. It represents the final objective of a major project which included an extensive research program at the Waltz Mill Forced-Cooling Test Facility. The formulas and procedures presented in this handbook are consistent with the results of this program as well as other full-scale and model investigations. Introductory material, including historical information, general design concepts and basic heat transfer and fluid dynamics theory is given for those who are unfamiliar with the fundamental principles of forced-cooling. The nucleus of the handbook consists of equations and calculating procedures for determining forced-cooled parameters and thermal/hydraulic performance levels. Both simple and sophisticated procedures have been provided, with the more complex procedures treating such areas as the thermal interaction between cable pipes as well as the transient aspects of ...

Energy Technology Data Exchange (ETDEWEB)

This document defines the requirements for the GPRS (General Packet Radio Service) infrastructure as part of the Dutch Smart Meter Specification. In this document all requirements originating from the NTA 8130, or additionally surveyed by a delegation of the Dutch grid operators, are presented in tables. Each requirement is tightly connected to one or more business use cases presented in the document. The ultimate goal of this procedure is to reduce ambiguity of the requirements due to a better understanding of the requirements.

Energy Technology Data Exchange (ETDEWEB)

This paper discussed issues related to the storage of electricity produced from renewable energy sources. Storage systems currently used by distribution systems were reviewed. The study showed that successful storage systems ensure high charging and discharging rates for shorter periods of time. Systems with high storage capacities must also be able to store energy for longer periods of time than traditional energy storage systems. Systems include pumped storage plants; electro-chemical accumulators; compressed air accumulators; and redox flow batteries. Hydrogen technologies for the storage of electric energy from renewable sources were reviewed. The paper also outlined energy storage and fuel cell research currently being conducted by researchers in the Czech Republic. The study concluded by stating that new energy storage technologies are required to ensure the reliability of electricity produced from renewable energy sources. 7 refs., 9 ...

Energy Technology Data Exchange (ETDEWEB)

This proceedings is the compilation of papers presented at the ninth PV Performance and Reliability Workshop held at the Sheraton Denver West Hotel on September 4--6, 1996. This years workshop included presentations from 25 speakers and had over 100 attendees. All of the presentations that were given are included in this proceedings. Topics of the papers included: defining service lifetime and developing models for PV module lifetime; examining and determining failure and degradation mechanisms in PV modules; combining IEEE/IEC/UL testing procedures; AC module performance and reliability testing; inverter reliability/qualification testing; standardization of utility interconnect requirements for PV systems; need activities to separate variables by testing individual components of PV systems (e.g. cells, modules, batteries, inverters,charge controllers) for individual reliability and then test them in actual system configurations; more results ...

Energy Technology Data Exchange (ETDEWEB)

Chromium electroplated AISI 316L stainless steel was nitrided using inductively coupled plasma (ICP) for application in the bipolar plate of a polymer electrolyte membrane fuel cell (PEMFC). A continuous and thin chromium nitride layer was formed at the surface of the samples after ICP nitriding for 2 h at 400 C. The interfacial contact resistance (ICR) and corrosion resistance in simulated PEMFC operating conditions were higher than the required values, while they varied with the applied dc bias voltage during the nitriding process. The ICR value decreased with an increase in bias voltage. Potentiodynamic polarization measurements showed that all of the nitrided samples had excellent corrosion resistance with a current density of {proportional_to}10{sup -7} A cm{sup -2} at the cathode. It was also found that the oxygen content at the surface was not increased after the corrosion test. X-ray diffractometry (XRD), field emission scanning ...

DEFF Research Database (Denmark)

Persistent hepatitis C virus (HCV) infection affects 170 million people worldwide. Acute HCV infection is often asymptomatic, but many infected individuals develop persistent infections that may lead to development of end-stage liver diseases, including liver cirrhosis and hepatocellular carcinoma. Thus, an HCV vaccine that could significantly lower the chronicity rate would have a major impact on the disease burden. Unfortunately, HCV is a highly mutable virus, and escape mutations can undermine vaccine-induced virus-specific immunity. Also, HCV exists as multiple genotypes, and so genotype-specific vaccines might be required to achieve broad protection. Finally, vaccine development has been hampered by the lack of a small animal model and cell culture systems, but these are currently being established. Despite these obstacles, several vaccine candidates tested in the chimpanzee HCV model have shown some encouraging results.

Energy Technology Data Exchange (ETDEWEB)

Options for successfully using biogas depend on project scale. Almost all biogas from anaerobic digesters must first go through a gas handling system that pressurizes, meters, and filters the biogas. Additional treatment, including hydrogen sulfide-mercaptan scrubbing, gas drying, and carbon dioxide removal may be necessary for specialized uses, but these are complex and expensive processes. Thus, they can be justified only for large-scale projects that require high-quality biogas. Small-scale projects (less than 65 cfm) generally use biogas (as produced) as a boiler fuel or for fueling internal combustion engine-generators to produce electricity. If engines or boilers are selected properly, there should be no need to remove hydrogen sulfide. Small-scale combustion turbines, steam turbines, and fuel cells are not used because of their technical complexity and high capital cost. Biogas cleanup to pipeline or transportation fuel specifications is ...

International Nuclear Information System (INIS)

This document provides the detailed design requirements for the Spent Nuclear Fuel Project Cold Vacuum Drying Facility. Process, safety, and quality assurance requirements and interfaces are specified.

International Nuclear Information System (INIS)

Carbon monoxide can be produced in severe accidents from interaction of ex-vessel molten core with concrete. Depending on the particular core-melt scenario, the type of concrete and geometric factors affecting the interaction, the quantities of carbon monoxide produced can vary widely, up to several volume percent in the containment. Carbon monoxide is a combustible gas. The carbon monoxide thus produced is in addition to the hydrogen produced by metal-water reactions and by radiolysis, and represents a possibly significant contribution to the combustible gas inventory in the containment. Assessment of possible accident loads to containment thus requires knowledge of the combustion properties of both CO and H_2 in the containment atmosphere. Extensive studies have been carried out and are still continuing in the nuclear industry to assess the threat of hydrogen in a severe reactor accident. However the contribution of carbon monoxide to the combustion threat has ...

Science.gov (United States)

In this study, we propose a new bioactive bone cement (BBC), composed of natural bone powder (hydroxyapatite; HA), chitosan powder, and the currently available polymethylmethacrylate (PMMA) bone cement, for use in orthopedic surgeries such as vertebroplasty or as bone filler. Three types of BBCs (BBC I, BBC II, and BBC III) were prepared with different composition ratios. In vitro tests and animal studies were performed with the new BBCs, and with a currently available commercial PMMA bone cement. Surface morphology, chemical composition, changes in pH over time, exothermic temperatures, intrusion, and cellular responses were investigated in vitro. Scanning electron microscopy (SEM) and radiological and histological examinations were performed in animal studies. The results showed that the major components of the BBCs were C, O, Ca, P, Cl, Si, S, Ba, and Mg. The pH values of the BBCs decreased after 1 day, but eventually recovered to 7.2-7.4. The water absorbency, weight loss, and ...

Energy Technology Data Exchange (ETDEWEB)

Eukaryotic genomes encode a zinc finger protein (ZPR1) with tandem ZPR1 domains. In response to growth stimuli, ZPR1 assembles into complexes with eukaryotic translation elongation factor 1A (eEF1A) and the survival motor neurons protein. To gain insight into the structural mechanisms underlying the essential function of ZPR1 in diverse organisms, we determined the crystal structure of a ZPR1 domain tandem and characterized the interaction with eEF1A. The ZPR1 domain consists of an elongation initiation factor 2-like zinc finger and a double-stranded {beta} helix with a helical hairpin insertion. ZPR1 binds preferentially to GDP-bound eEF1A but does not directly influence the kinetics of nucleotide exchange or GTP hydrolysis. However, ZPR1 efficiently displaces the exchange factor eEF1B from preformed nucleotide-free complexes, suggesting that it may function as a negative regulator of eEF1A activation. Structure-based mutational and complementation analyses reveal a conserved binding ...

Energy Technology Data Exchange (ETDEWEB)

To predict the response of blood lead to airborne and dietary lead requires knowledge of the rate of uptake of lead into the body from lung and gut, its subsequent partitioning between compartments, the stay time in those compartments, and its redistribution or excretion. Tracer studies with volunteers have shown no differences in systemic distribution of inorganic lead between tissues whether it is taken by inhalation, ingestion or injection. Lead is rapidly transferred from plasma to red cells, and there is slower movement thence into liver and other soft tissues, to bone, and to excreta. Work at Harwell and elsewhere with /sup 203/Pb has shown that the initial rapid distribution leaves rather over half the assimilated lead attached to red cells. The result is remarkably consistent, and applies also to dogs and baboons. The renal clearance (Vu) (ratio of U to CB, or daily urinary output expressed as mass of blood having ...

Energy Technology Data Exchange (ETDEWEB)

Electric and hybrid-electric vehicles are being developed and commercialized around the world at a rate never before seen. These efforts are driven by the prospect of vehicles with lower emissions and higher fuel efficiencies. The widespread adaptation of such vehicles promises a cleaner environment and a reduction in the rate of accumulation of greenhouse gases, Critical to the success of this technology is the use of electrochemical power sources such as batteries and fuel cells, which can convert chemical energy to electrical energy more efficiently and quietly than internal combustion engines. This overview will concentrate on the work being conducted in the US to develop advanced propulsion systems for the electric and hybrid vehicles, This work is spearheaded by the US Advanced Battery Consortium (USABC) for electric vehicles and the Partnership for a New Generation of Vehicle (PNGV) for hybrid-electric vehicles, both of which can be read about on the world ...

Energy Technology Data Exchange (ETDEWEB)

Investigated here were the effects of microwave (MW) radiation (2450-MHz, continuous-wave, mean specific absorption rate of 103.5 + or - 4.2 W/kg) and convention heating on the nonphosphorylating oxidative metabolism of human peripheral mononuclear leukocytes (96% lymphocytes, 4% monocytes) at 37 C. Metabolic activity, determined by chemiluminescence (CL) of cells challenged with luminol (5-aminO-2, 3-dihydro-1, 4-phthalazinedione) linked to bovine serum albumin, was detected with a brightness photomer. A significant stimulation after after MW exposure (p < 0.005) over total CL of matched 37 C-incubator controls was observed. A similar degree of stimulation, compared to incubator controls, was also detected after sham treatment. No significant difference existed between changes in total CL or stimulation indices of the MW and sham-exposed groups. Exposure to MW radiation, under normothermic (37 + or - 0.03 C) conditions, appears to have no effect on the ...

Science.gov (United States)

The goal of the present work was to determine the impact of N3-methyladenine (3-mA), an important lesion generated by many environmental agents and anticancer drugs, on in vivo DNA replication and in vitro RNA transcription. Due to 3-mA chemical instability, the stable isostere 3-methyl-3-deazaadenine (3-m-c(3)A) was site specifically positioned into an oligodeoxynucleotide. The oligomer was, then incorporated into a vector system that is rapidly converted to ssDNA inside yeast cells and requires DNA replication opposite the lesion for plasmid clonal selection. For control purposes, an adenine or a stable apurinic/apyrimidinic (AP)-lesion was placed at the same site. The presence of each lesion in the oligonucleotide was confirmed by MALDI-TOF analysis. Plasmids were then transfected into yeast cells. While the AP-site dramatically reduced plasmid replication in all strains, the 3-m-c(3)A had a slight effect in the rad30 ...

Science.gov (United States)

Assessment of the immune system's capability to respond to antigens with the generation of specific antibodies, whilst under the influence of a test article, is required in toxicity tests according to the European guideline for repeated dose toxicity testing of medicinal products. The purpose of this study in rats was to validate methodology for the determination of Keyhole Limpet Haemocyanin (KLH)-specific antibodies under the influence of an immunologically active compound. The immunosuppressant FK506, commercially available as Prograf, was administered orally (gavage) to five rats per sex per group at dosages of 0.5mg/kg per day or 3mg/kg per day, for a period of 4 weeks. On days 14 and 22, KLH was administered subcutaneously, with an adjuvant (AluGel), to the two treated groups and a control (i.e. without FK506 treatment) approximately 1h following administration of FK506. Terminal investigations included haematology parameters, titration of KLH-specific ...

Energy Technology Data Exchange (ETDEWEB)

The availability of polysilicon feedstock has become a major issue for the photovoltaic (PV) industry in recent years. Most of the current polysilicon feedstock is derived from rejected material from the semiconductor industry. However, the reject material can become scarce and more expensive during periods of expansion in the integrated-circuit industry. Continued rapid expansion of the PV crystalline-silicon industry will eventually require a dedicated supply of polysilicon feedstock to produce solar cells at lower costs. The photovoltaic industry can accept a lower purity polysilicon feedstock (solar-grade) compared to the semiconductor industry. The purity requirements and potential production techniques for solar-grade polysilicon have been reviewed. One interesting process from previous research involves reactive gas blowing of the molten silicon charge. As an example, Dosaj et all reported a reduction of metal and ...

Wastenet

The nature of any pollution abatement measures that are required in operation.

Science.gov (United States)

...requirements for Federal information technology resources. 352.239-72...requirements for Federal information technology resources. As prescribed...Requirements for Federal Information Technology Resources (January...

Science.gov (United States)

...false Payment required for O. and C. timber. 2812.5-1 Section 2812.5-1...5-1 Payment required for O. and C. timber. An applicant will be required...officer of the estimated volume of all timber to be cut, removed, or destroyed,...

Science.gov (United States)

... Task Force Commander's Handbook for Domestic ... and Radiological (CBR) Defense Requirements Supporting ... DC: Department of Navy, May 1996 ...

Science.gov (United States)

... Accession Number : ADD808679. Title : Advanced Composites for Aerospace Require Waterjet Technology. Descriptive Note : Journal article. ...

British Library Electronic Table of Contents (United Kingdom)

Introduction: Haploidentical transplantation, with extensive T cell depletion to prevent GvHD, is associated with a high incidence of infection-related deaths. The key challenge is to improve immune recovery with allogeneic donor T cells without triggering GvHD. As T regulatory cells (Tregs) controlled GvHD in pre-clinical studies, the present study evaluated the impact of an infusion of donor CD4/CD25 + Tregs, followed by an inoculum of donor mature T cells (Tcons) and positively immunoselected CD34 + cells in the setting of haploidentical stem cell transplantation. Patients and methods: Twenty-eight patients were enrolled in this study (22 AML; 5 ALL; 1 NHL). All received immunoselected Tregs (CliniMACS, Miltenyi Biotec) followed by positively immunoselected CD34 + cells together with Tc...