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Sample records for accept unmethylated dna

  1. COLD-PCR amplification of bisulfite-converted DNA allows the enrichment and sequencing of rare un-methylated genomic regions.

    Castellanos-Rizaldos, Elena; Milbury, Coren A; Karatza, Elli; Chen, Clark C; Makrigiorgos, G Mike; Merewood, Anne

    2014-01-01

    Aberrant hypo-methylation of DNA is evident in a range of human diseases including cancer and diabetes. Development of sensitive assays capable of detecting traces of un-methylated DNA within methylated samples can be useful in several situations. Here we describe a new approach, fast-COLD-MS-PCR, which amplifies preferentially un-methylated DNA sequences. By employing an appropriate denaturation temperature during PCR of bi-sulfite converted DNA, fast-COLD-MS-PCR enriches un-methylated DNA and enables differential melting analysis or bisulfite sequencing. Using methylation on the MGMT gene promoter as a model, it is shown that serial dilutions of controlled methylation samples lead to the reliable sequencing of un-methylated sequences down to 0.05% un-methylated-to-methylated DNA. Screening of clinical glioma tumor and infant blood samples demonstrated that the degree of enrichment of un-methylated over methylated DNA can be modulated by the choice of denaturation temperature, providing a convenient method for analysis of partially methylated DNA or for revealing and sequencing traces of un-methylated DNA. Fast-COLD-MS-PCR can be useful for the detection of loss of methylation/imprinting in cancer, diabetes or diet-related methylation changes. PMID:24728321

  2. Genome-wide tracking of unmethylated DNA Alu repeats in normal and cancer cells

    Rodriguez, Jairo; Vives, Laura; Jordà, Mireia;

    2008-01-01

    . Normal colon epithelial cells contain in average 25 486 +/- 10 157 unmethylated Alu's per haploid genome, while in tumor cells this figure is 41 995 +/- 17 187 (P = 0.004). There is an inverse relationship in Alu families with respect to their age and methylation status: the youngest elements exhibit the...

  3. Anatomic localization of O6-methylguanine DNA methyltransferase (MGMT) promoter methylated and unmethylated tumors: a radiographic study in 358 de novo human glioblastomas.

    Ellingson, Benjamin M; Cloughesy, Timothy F; Pope, Whitney B; Zaw, Taryar M; Phillips, Heidi; Lalezari, Shadi; Nghiemphu, Phioanh L; Ibrahim, Hassana; Naeini, Kourosh M; Harris, Robert J; Lai, Albert

    2012-01-16

    Promoter methylation of O6-methylguanine DNA methyltransferase (MGMT) is associated with a favorable prognosis in glioblastoma multiforme (GBM) and has been hypothesized to occur early in tumor transformation of glial cells. Thus, a possible link exists between the site of malignant transformation and MGMT promoter methylation status. Using the Analysis of Differential Involvement (ADIFFI) statistical mapping technique in a total of 358 patients with GBM, we demonstrate that human de novo GBMs occur in a high frequency contiguous with the posterior subventricular zone (SVZ); MGMT promoter methylated GBMs are lateralized to the left hemisphere, while MGMT unmethylated GBMs are lateralized to the right hemisphere; and tumors near the left temporal lobe have a significantly longer overall survival compared with tumors occurring elsewhere, independent of treatment or MGMT methylation status. PMID:22001163

  4. GC skew is a conserved property of unmethylated CpG island promoters across vertebrates.

    Hartono, Stella R; Korf, Ian F; Chédin, Frédéric

    2015-11-16

    GC skew is a measure of the strand asymmetry in the distribution of guanines and cytosines. GC skew favors R-loops, a type of three stranded nucleic acid structures that form upon annealing of an RNA strand to one strand of DNA, creating a persistent RNA:DNA hybrid. Previous studies show that GC skew is prevalent at thousands of human CpG island (CGI) promoters and transcription termination regions, which correspond to hotspots of R-loop formation. Here, we investigated the conservation of GC skew patterns in 60 sequenced chordates genomes. We report that GC skew is a conserved sequence characteristic of the CGI promoter class in vertebrates. Furthermore, we reveal that promoter GC skew peaks at the exon 1/ intron1 junction and that it is highly correlated with gene age and CGI promoter strength. Our data also show that GC skew is predictive of unmethylated CGI promoters in a range of vertebrate species and that it imparts significant DNA hypomethylation for promoters with intermediate CpG densities. Finally, we observed that terminal GC skew is conserved for a subset of vertebrate genes that tend to be located significantly closer to their downstream neighbors, consistent with a role for R-loop formation in transcription termination. PMID:26253743

  5. Parp1 localizes within the Dnmt1 promoter and protects its unmethylated state by its enzymatic activity.

    Michele Zampieri

    Full Text Available BACKGROUND: Aberrant hypermethylation of CpG islands in housekeeping gene promoters and widespread genome hypomethylation are typical events occurring in cancer cells. The molecular mechanisms behind these cancer-related changes in DNA methylation patterns are not well understood. Two questions are particularly important: (i how are CpG islands protected from methylation in normal cells, and how is this protection compromised in cancer cells, and (ii how does the genome-wide demethylation in cancer cells occur. The latter question is especially intriguing since so far no DNA demethylase enzyme has been found. METHODOLOGY/PRINCIPAL FINDINGS: Our data show that the absence of ADP-ribose polymers (PARs, caused by ectopic over-expression of poly(ADP-ribose glycohydrolase (PARG in L929 mouse fibroblast cells leads to aberrant methylation of the CpG island in the promoter of the Dnmt1 gene, which in turn shuts down its transcription. The transcriptional silencing of Dnmt1 may be responsible for the widespread passive hypomethylation of genomic DNA which we detect on the example of pericentromeric repeat sequences. Chromatin immunoprecipitation results show that in normal cells the Dnmt1 promoter is occupied by poly(ADP-ribosylated Parp1, suggesting that PARylated Parp1 plays a role in protecting the promoter from methylation. CONCLUSIONS/SIGNIFICANCE: In conclusion, the genome methylation pattern following PARG over-expression mirrors the pattern characteristic of cancer cells, supporting our idea that the right balance between Parp/Parg activities maintains the DNA methylation patterns in normal cells. The finding that in normal cells Parp1 and ADP-ribose polymers localize on the Dnmt1 promoter raises the possibility that PARylated Parp1 marks those sequences in the genome that must remain unmethylated and protects them from methylation, thus playing a role in the epigenetic regulation of gene expression.

  6. Active DNA Demethylation Mediated by DNA Glycosylases

    Zhu, Jian-Kang

    2009-01-01

    Active DNA demethylation is involved in many vital developmental and physiological processes of plants and animals. Recent genetic and biochemical studies in Arabidopsis have demonstrated that a subfamily of DNA glycosylases function to promote DNA demethylation through a base excision-repair pathway. These specialized bifunctional DNA glycosylases remove the 5-methylcytosine base and then cleave the DNA backbone at the abasic site, resulting in a gap that is then filled with an unmethylated ...

  7. High-mobility group AT-hook protein 2 expression and its prognostic significance in MGMT methylated and unmethylated glioblastoma.

    Schwarm, Frank P; Uhle, Florian; Schänzer, Anne; Acker, Till; Stein, Marco; Reinges, Marcus H T; Weischer, Cornelia; Weigand, Marcus A; Uhl, Eberhard; Kolodziej, Malgorzata A

    2016-04-01

    High-mobility group AT-hook protein 2 (HMGA 2) is a transcription factor associated with malignancy and poor prognosis in a variety of human cancers. We correlated HMGA 2 expression with clinical parameters, survival, and O-6-methylguanine-DNA methyltransferase methylation status (MGMT) in glioblastoma patients. HMGA 2 expression was determined by performing quantitative real-time polymerase chain reaction (qPCR) and immunohistochemistry (IHC) in 44 glioblastoma patients and 5 non-tumorous brain specimens as controls. Gene expression levels of MGMT methylated vs. unmethylated patients, and gene expression levels between patient groups, both for qPCR and IHC data were compared using the Mann-Whitney U test. The relationship between HMGA 2 expression, progression-free survival and overall survival was analyzed using the Kaplan-Meier method and the log-rank test. P-values of <0.05 were considered statistically significant throughout the analyses. The mean age of patients at diagnosis was 57.4±15.7 years, and the median survival was 16 months (SE 2.8; 95% CI, 10.6-21.4). HMGA 2 gene expression was significantly higher in glioblastoma compared to normal brain tissue on qPCR (mean, 0.35; SD, 0.27 vs. 0.03, SD, 0.05) and IHC levels (IRS mean, 17.21; SD, 7.43 vs. 3.20; SD, 1.68) (p=0.001). Survival analysis revealed that HMGA 2 overexpression was associated with a shorter progression-free and overall survival time in patients with methylation (n=24). The present study shows a tendency that HMGA 2 overexpression correlates with a poor prognosis of glioblastoma patients independent of MGMT methylation status. The results suggest that HMGA 2 could play an important role in the treatment of glioblastoma and could have a function in prognosis of this type of cancer. PMID:26892260

  8. "Would you accept having your DNA profile inserted in the National Forensic DNA database? Why?" Results of a questionnaire applied in Portugal.

    Machado, Helena; Silva, Susana

    2014-01-01

    The creation and expansion of forensic DNA databases might involve potential threats to the protection of a range of human rights. At the same time, such databases have social benefits. Based on data collected through an online questionnaire applied to 628 individuals in Portugal, this paper aims to analyze the citizens' willingness to donate voluntarily a sample for profiling and inclusion in the National Forensic DNA Database and the views underpinning such a decision. Nearly one-quarter of the respondents would indicate 'no', and this negative response increased significantly with age and education. The overriding willingness to accept the inclusion of the individual genetic profile indicates an acknowledgement of the investigative potential of forensic DNA technologies and a relegation of civil liberties and human rights to the background, owing to the perceived benefits of protecting both society and the individual from crime. This rationale is mostly expressed by the idea that all citizens should contribute to the expansion of the National Forensic DNA Database for reasons that range from the more abstract assumption that donating a sample for profiling would be helpful in fighting crime to the more concrete suggestion that everyone (criminals and non-criminals) should be in the database. The concerns with the risks of accepting the donation of a sample for genetic profiling and inclusion in the National Forensic DNA Database are mostly related to lack of control and insufficient or unclear regulations concerning safeguarding individuals' data and supervising the access and uses of genetic data. By providing an empirically-grounded understanding of the attitudes regarding willingness to donate voluntary a sample for profiling and inclusion in a National Forensic DNA Database, this study also considers the citizens' perceived benefits and risks of operating forensic DNA databases. These collective views might be useful for the formation of international common

  9. A phase II study of bevacizumab and erlotinib after radiation and temozolomide in MGMT unmethylated GBM patients.

    Raizer, J J; Giglio, P; Hu, J; Groves, M; Merrell, R; Conrad, C; Phuphanich, S; Puduvalli, V K; Loghin, M; Paleologos, N; Yuan, Y; Liu, D; Rademaker, A; Yung, W K; Vaillant, B; Rudnick, J; Chamberlain, M; Vick, N; Grimm, S; Tremont-Lukats, I W; De Groot, J; Aldape, K; Gilbert, M R

    2016-01-01

    Survival for glioblastoma (GBM) patients with an unmethyated MGMT promoter in their tumor is generally worse than methylated MGMT tumors, as temozolomide (TMZ) response is limited. How to better treat patients with unmethylated MGMT is unknown. We performed a trial combining erlotinib and bevacizumab in unmethylated GBM patients after completion of radiation (RT) and TMZ. GBM patients with an unmethylated MGMT promoter were trial eligible. Patient received standard RT (60 Gy) and TMZ (75 mg/m2 × 6 weeks) after surgical resection of their tumor. After completion of RT they started erlotinib 150 mg daily and bevacizumab 10 mg/kg every 2 weeks until progression. Imaging evaluations occurred every 8 weeks. The primary endpoint was overall survival. Of the 48 unmethylated patients enrolled, 46 were evaluable (29 men and 17 women); median age was 55.5 years (29-75) and median KPS was 90 (70-100). All patients completed RT with TMZ. The median number of cycles (1 cycle was 4 weeks) was 8 (2-47). Forty-one patients either progressed or died with a median progression free survival of 9.2 months. At a follow up of 33 months the median overall survival was 13.2 months. There were no unexpected toxicities and most observed toxicities were categorized as CTC grade 1 or 2. The combination of erlotinib and bevacizumab is tolerable but did not meet our primary endpoint of increasing survival. Importantly, more trials are needed to find better therapies for GBM patients with an unmethylated MGMT promoter. PMID:26476729

  10. MIF-CD74 GUIDED THERAPEUTIC STRATEGY FOR THE UPFRONT TREATMENT OF GBM PATIENTS WITH AN UNMETHYLATED MGMT PROMOTER

    McDonald, Kerrie L; Ha, Wendy; Sevim, Hatice

    2014-01-01

    BACKGROUND: Promoter methylation of the DNA repair gene, MGMT is associated with improved treatment outcome for newly diagnosed glioblastoma (GBM) treated with standard chemoradiation. Currently, there are no universally effective systemic treatments available for patients with an unmethylated MGMT promoter or treatments for patients whose tumor recurs after primary treatment, irrespective of MGMT methylation status. We used SELDI-TOF MS to identify low molecular weight proteins associated with poor survival. We report a new therapeutic strategy with potential to overcome the drug resistance conferred by MGMT when applied as an adjunct therapy to the standard chemoradiation for patients. METHODS: We screened the low molecular weight proteome of patients' tumors, which were non-responsive to chemoradiotherapy (survival <12 months). Proteins of significant abundance in the non-responsive patients were purified, identified and validated in a larger cohort of GBM (n = 230). Inhibitors were sourced and efficacy of the drug was tested in patient derived tumor models. RESULTS: High expression of the inflammatory related protein Macrophage Inhibitory Factor (MIF) and its receptor, CD74 were found in patients who were non-responsive to chemoradiation. Heightened expression of both MIF and CD74 were identified in 57% of GBM (n = 230) and co-segregated with poor prognosis. CD74 forms a complex with CD44 at the cell surface. After MIF binds to the CD74/44 complex, Src is recruited. Src recruitment then initiates the activation of multiple pathways including Ras/MAPK and Akt resulting in tumor proliferation. Pro-angiogenic effects have also been attributed to this complex. Because of its involvement in tumorigenesis, inhibition of the MIF/CD74/CD44 complex with specific inhibitors in combination with chemoradiotherapy will likely abate the resistance to standard treatment and result in longer survival. Ibudilast (AV411; 3-isobutyryl-2-isopropylpyrazolo-[1,5-a] pyridine) is an

  11. Epigenetic Characterization of the FMR1 Promoter in Induced Pluripotent Stem Cells from Human Fibroblasts Carrying an Unmethylated Full Mutation

    Celine E.F. de Esch

    2014-10-01

    Full Text Available Silencing of the FMR1 gene leads to fragile X syndrome, the most common cause of inherited intellectual disability. To study the epigenetic modifications of the FMR1 gene during silencing in time, we used fibroblasts and induced pluripotent stem cells (iPSCs of an unmethylated full mutation (uFM individual with normal intelligence. The uFM fibroblast line carried an unmethylated FMR1 promoter region and expressed normal to slightly increased FMR1 mRNA levels. The FMR1 expression in the uFM line corresponds with the increased H3 acetylation and H3K4 methylation in combination with a reduced H3K9 methylation. After reprogramming, the FMR1 promoter region was methylated in all uFM iPSC clones. Two clones were analyzed further and showed a lack of FMR1 expression, whereas the presence of specific histone modifications also indicated a repressed FMR1 promoter. In conclusion, these findings demonstrate that the standard reprogramming procedure leads to epigenetic silencing of the fully mutated FMR1 gene.

  12. Immunostimulatory DNA sequences influence the course of adjuvant arthritis

    Ronaghy, A; Prakken, BJ; Takabayashi, K; Firestein, GS; Boyle, D; Zvailfler, NJ; Roord, STA; Albani, S; Carson, DA; Raz, E

    2002-01-01

    Bacterial DNA is enriched in unmethylated CpG motifs that have been shown to activate the innate immune system. These immunostimulatory DNA sequences (ISS) induce inflammation when injected directly into joints. However, the role of bacterial DNA in systemic arthritis is not known. The purpose of th

  13. Homogeneous MGMT immunoreactivity correlates with an unmethylated MGMT promoter status in brain metastases of various solid tumors.

    Barbara Ingold

    Full Text Available The O(6-methylguanine-methyltransferase (MGMT promoter methylation status is a predictive parameter for the response of malignant gliomas to alkylating agents such as temozolomide. First clinical reports on treating brain metastases with temozolomide describe varying effects. This may be due to the fact that MGMT promoter methylation of brain metastases has not yet been explored in depth. Therefore, we assessed MGMT promoter methylation of various brain metastases including those derived from lung (n = 91, breast (n = 72 kidney (n = 49 and from malignant melanomas (n = 113 by methylation-specific polymerase chain reaction (MS-PCR and MGMT immunoreactivity. Fifty-nine of 199 brain metastases (29.6% revealed a methylated MGMT promoter. The methylation rate was the highest in brain metastases derived from lung carcinomas (46.5% followed by those from breast carcinoma (28.8%, malignant melanoma (24.7% and from renal carcinoma (20%. A significant correlation of homogeneous MGMT-immunoreactivity (>95% MGMT positive tumor cells and an unmethylated MGMT promoter was found. Promoter methylation was detected in 26 of 61 (43% tumors lacking MGMT immunoreactivity, in 17 of 63 (27% metastases with heterogeneous MGMT expression, but only in 5 of 54 brain metastases (9% showing a homogeneous MGMT immunoreactivity. Our results demonstrate that a significant number of brain metastases reveal a methylated MGMT-promoter. Based on an obvious correlation between homogeneous MGMT immunoreactivity and unmethylated MGMT promoter, we hypothesize that immunohistochemistry for MGMT may be a helpful diagnostic tool to identify those tumors that probably will not benefit from the use of alkylating agents. The discrepancy between promoter methylation and a lack of MGMT immunoreactivity argues for assessing MGMT promoter methylation both by immunohistochemical as well as by molecular approaches for diagnostic purposes.

  14. Acceptance of non-invasive prenatal testing by cell free foetal DNA for foetal aneuploidy in a developing country: experience at a tertiary care centre in India

    Namrata Kashyap

    2016-03-01

    Conclusions: Newer genomic technology involving cell free maternal DNA is a new storm in prenatal diagnosis. Its application in clinical practice is the need of the hour, however, the lack of awareness, high cost and unavailability of the test in the country appears to be a major limiting factor for its poor acceptability. [Int J Reprod Contracept Obstet Gynecol 2016; 5(3.000: 705-710

  15. “Would you accept having your DNA profile inserted in the National Forensic DNA database? Why?” Results of a questionnaire applied in Portugal

    Machado, Helena; Silva, Susana, org.

    2014-01-01

    The creation and expansion of forensic DNA databases might involve potential threats to the protection of a range of human rights. At the same time, such databases have social benefits. Based on data collected through an online questionnaire applied to 628 individuals in Portugal, this paper aims to analyze the citizens’ willingness to donate voluntarily a sample for profiling and inclusion in the National Forensic DNA Database and the views underpinning such a decision. Nearly one-quarte...

  16. Acceptance of non-invasive prenatal testing by cell free foetal DNA for foetal aneuploidy in a developing country: experience at a tertiary care centre in India

    Namrata Kashyap; Mandakini Pradhan; Piyush Kumar; Neeta Singh

    2016-01-01

    Background: Non-invasive prenatal testing is a new technique which is deepening its root all over the world. Its tremendous potential lies in its ability of using cell free fetal DNA from the plasma of pregnant women. However, to what extent the technology has reached to a common person is also to be given a thought. hence the study was planned to assess the acceptability of non-invasive prenatal testing in Indian settings, to study about the awareness and baseline knowledge about Down's synd...

  17. Triplex-mediated analysis of cytosine methylation at CpA sites in DNA

    Johannsen, Marie W.; Gerrard, Simon R.; Melvin, Tracy; Brown, Tom

    2014-01-01

    Modified triplex-forming oligonucleotides distinguish 5-methyl cytosine from unmethylated cytosine in DNA duplexes by differences in triplex melting temperatures. The discrimination is sequence-specific; dramatic differences in stabilisation are seen for CpA methylation, whereas CpG methylation is not detected. This direct detection of DNA methylation constitutes a new approach for epigenetic analysis.

  18. Cilengitide with metronomic temozolomide, procarbazine, and standard radiotherapy in patients with glioblastoma and unmethylated MGMT gene promoter in ExCentric, an open-label phase II trial.

    Khasraw, Mustafa; Lee, Adrian; McCowatt, Sally; Kerestes, Zoltan; Buyse, Marc E; Back, Michael; Kichenadasse, Ganessan; Ackland, Stephen; Wheeler, Helen

    2016-05-01

    Newly diagnosed glioblastoma multiforme with unmethylated MGMT promoter has a poor prognosis, with a median survival of 12 months. This phase II study investigated the efficacy and safety of combining the selective integrin inhibitor cilengitide with a combination of metronomic temozolomide and procarbazine for these patients. Eligible patients (newly diagnosed, histologically confirmed supratentorial glioblastoma with unmethylated MGMT promoter) were entered into this multicentre study. Cilengitide (2000 mg IV twice weekly) was commenced 1 week prior to radiotherapy combined with daily temozolomide (60 mg/m(2)) and procarbazine (50 or 100 mg) and, after 4 weeks' break, followed by six adjuvant cycles of temozolomide (50-60 mg/m(2)) and procarbazine (50 or 100 mg) on days 1-20, every 28 days. Cilengitide was continued for up to 12 months or until disease progression or unacceptable toxicity. The primary endpoint for efficacy was a 12-month overall survival rate of 65 %. Twenty-nine patients completed study treatment. Sixteen patients survived for 12 months or more, an overall survival rate of 55 %. The median overall survival was 14.5 months (95 % CI 11.1-19.6) and the median progression-free survival was 7.4 months (95 % CI 6.1-8). Cilengitide combined with metronomic temozolomide and procarbazine in MGMT-promoter unmethylated glioblastoma did not improve survival compared with historical data and does not warrant further investigation. PMID:26935578

  19. Sequence-nonspecific replication of transfected plasmid DNA in poxvirus-infected cells.

    DeLange, A. M.; McFadden, G

    1986-01-01

    A system in which transfected plasmid DNA replicates in the cytoplasm of poxvirus-infected cells is described. A variety of recombinant plasmids was introduced into poxvirus-infected cells by transfection, and replication of input plasmid DNA was monitored by (i) digestion with restriction enzymes that discriminate between input methylated plasmid DNA and unmethylated DNA produced by replication in mammalian cells; (ii) amplification of intracellular plasmid DNA; and (iii) density shift analy...

  20. Quantitation of DNA methyltransferase activity via chronocoulometry in combination with rolling chain amplification.

    Ji, Jingjing; Liu, Yuanjian; Wei, Wei; Zhang, Yuanjian; Liu, Songqin

    2016-11-15

    In this paper, a rolling chain amplification (RCA) strategy was proposed for chronocoulometric detection of DNA methyltransferase (MTase) activity. Briefly, after the double DNA helix structure was assembled on the surface of gold electrode, it was first methylated by M. SssI MTase and then RCA was realized in the presence of E. coli and phi29 DNA polymerase. Successively, numerous hexaammineruthenium (III) chloride ([Ru(NH3)6)(3+), RuHex) were adsorbed on replicons by electrostatic interaction and generated a large electrochemical readout, the signal was "on". On the contrary, in the absence of M. SssI MTase, the methylated CpG site in the unmethylated double DNA helix structure could be specifically recognized and cleaved by HpaII, resulting in a disconnection of RCA from the electrode. This led seldom RuHex to be absorbed onto the surface of electrode, the signal was "off". Based on the proposed strategy, the activity of M. SssI MTase was assayed in the range of 0.5-60U/mL with a detection limit of 0.09U/mL (S/N=3). In addition, the inhibition of procaine and epicatechin on M. SssI MTase activity was evaluated. When the proposed method was applied in complex matrix such as human serum samples, acceptable accuracy, precision and high sensitivity were achieved. Therefore, the proposed method was a potential useful mean for clinical diagnosis and drug development. PMID:27155113

  1. The Escherichia coli regulatory protein OxyR discriminates between methylated and unmethylated states of the phage Mu mom promoter.

    Bölker, M; Kahmann, R

    1989-01-01

    Expression of the phage Mu mom gene is transcriptionally regulated by DNA methylation. Three GATC sites upstream of the mom promoter have to be methylated by the Escherichia coli deoxyadenosine methylase (Dam) to allow initiation of transcription. An E. coli dam strain was mutagenized with Tn5 in an attempt to isolate mutants which allow mom gene expression. Three independent Tn5 mutants were isolated, each mapped to a gene at 89.6 min which we designate momR. The wildtype gene was cloned and...

  2. Effect of alternative temozolomide schedules on glioblastoma O 6-methylguanine-DNA methyltransferase activity and survival

    Robinson, C G; Palomo, J M; Rahmathulla, G; McGraw, M; Donze, J; L. Liu; Vogelbaum, M A

    2010-01-01

    Background: O 6-methylguanine-DNA methyltransferase (MGMT) expression in glioblastoma correlates with temozolomide resistance. Dose-intense temozolomide schedules deplete MGMT activity in peripheral blood mononuclear cells; however, no published data exist evaluating the effect of temozolomide schedules on intracranial tumour MGMT activity. Methods: Human glioblastoma cells (GBM43) with an unmethylated MGMT promoter were implanted intracranially in immunodeficient rodents. Three weeks later, ...

  3. Hydrophobicity of methylated DNA as a possible mechanism for gene silencing

    AFM images show that chromatin reconstituted on methylated DNA (meDNA) is compacted when imaged under water. Chromatin reconstituted on unmethylated DNA is less compacted and less sensitive to hydration. These differences must reflect changes in the physical properties of DNA on methylation, but prior studies have not revealed large differences between methylated and unmethylated DNA. Quasi-elastic light scattering studies of solutions of methylated and unmethylated DNA support this view. In contrast, AFM images of molecules at a water/solid interface yield a persistence length that nearly doubles (to 92.5 ± 4 nm) when 9% of the total DNA is methylated. This increase in persistence length is accompanied by a decrease in contour length, suggesting that a significant fraction of the meDNA changes into the stiffer A form as the more hydrophobic meDNA is dehydrated at the interface. This suggests a simple mechanism for gene silencing as the stiffer meDNA is more difficult to remove from nucleosomes. (paper)

  4. Acceptable risk

    The hazards of nuclear power, radioactive wastes and radiation are analysed in a general book describing and defining acceptable-risk problems, the difficulties in resolving them and considering such issues as uncertainty about their definition, lack of relevant facts, conflicting and conflicted social values and disagreements between technical experts and the lay public. The many methods that have been proposed for resolving acceptable-risk problems are identified and criticised. (author)

  5. The PHD finger of human UHRF1 reveals a new subgroup of unmethylated histone H3 tail readers.

    Nada Lallous

    Full Text Available The human UHRF1 protein (ubiquitin-like containing PHD and RING finger domains 1 has emerged as a potential cancer target due to its implication in cell cycle regulation, maintenance of DNA methylation after replication and heterochromatin formation. UHRF1 functions as an adaptor protein that binds to histones and recruits histone modifying enzymes, like HDAC1 or G9a, which exert their action on chromatin. In this work, we show the binding specificity of the PHD finger of human UHRF1 (huUHRF1-PHD towards unmodified histone H3 N-terminal tail using native gel electrophoresis and isothermal titration calorimetry. We report the molecular basis of this interaction by determining the crystal structure of huUHRF1-PHD in complex with the histone H3 N-terminal tail. The structure reveals a new mode of histone recognition involving an extra conserved zinc finger preceding the conventional PHD finger region. This additional zinc finger forms part of a large surface cavity that accommodates the side chain of the histone H3 lysine K4 (H3K4 regardless of its methylation state. Mutation of Q330, which specifically interacts with H3K4, to alanine has no effect on the binding, suggesting a loose interaction between huUHRF1-PHD and H3K4. On the other hand, the recognition appears to rely on histone H3R2, which fits snugly into a groove on the protein and makes tight interactions with the conserved aspartates D334 and D337. Indeed, a mutation of the former aspartate disrupts the formation of the complex, while mutating the latter decreases the binding affinity nine-fold.

  6. Nasal delivery of Japanese cedar pollen Cryj1 by using self-gelling immunostimulatory DNA for effective induction of immune responses in mice.

    Ishii-Mizuno, Yumiko; Umeki, Yuka; Takahashi, Yuki; Kato, Yukinori; Takabayashi, Tetsuji; Fujieda, Shigeharu; Takakura, Yoshinobu; Nishikawa, Makiya

    2015-01-01

    To develop an immunotherapeutic vaccine for treatment of allergic rhinitis, we developed a controlled release formulation of Cryj1, a major Japanese cedar pollen allergen, with immunostimulatory potency. Two sets of hexapod-like structured DNA (hexapodna) were prepared using six oligodeoxynucleotides (ODNs) each, including ODNs with an unmethylated cytosine-phosphate-guanine (CpG) sequence (CpG motif), to obtain an immunostimulatory DNA hydrogel (sDNA hydrogel). A non-immunostimulatory DNA hy...

  7. Bifidobacteria DNA Induces Murine Macrophages Activation in vitro

    Yalin Li; Xun Qu; Hua Yang; Li Kang; Yingping Xu; Bo Bai; Wengang Song

    2005-01-01

    Previous studies have shown that oligodeoxynucleotides containing unmethylated CpG motifs were used as adjuvants for immunoregulation and immune response. This study was to explore the activation effects of Bifidobacteria DNA containing unmethylated CpG motifs (CpG DNA) on murine macrophage J774A.1 cells. The genomic DNA of Bifidobacteria was extracted and purified, and the methylation degree of CpG motifs was tested.The phagocytic ability of the macrophages was detected by flow cytometry. The cytokines (IL-1β, IL-6, IL-12p40 and TNF-α) levels in the culture supernatants of Bifidobacteria DNA treated J774A.1 cells were assayed by ELISA. The content of nitric oxide (NO) was detected by Griess reagent. After treated with Bifidobacteria DNA for 24h,Nile Red stain increased in J774A.1 macrophage, which suggested that the lipid metabolism increased in the macrophages. The phagocytic ability and levels of NO and cytokines of IL-1β, IL-6, IL-12p40 and TNF-α were significantly higher than PBS group and CT DNA group. The results indicated that Bifidobacteria DNA could activate murine macrophages J774A.1, which could provide scientific basis for the research and application of microorganism DNA preparation.

  8. Acceptability, acceptance and decision making

    There is a fundamental difference between the acceptability of a civilizatory or societal risk and the acceptability of the decision-making process that leads to a civilizatory or societal risk. The analysis of individual risk decisions - regarding who, executes when which indisputably hazardous, unhealthy or dangerous behaviour under which circumstances - is not helpful in finding solutions for the political decisions at hand in Germany concerning nuclear energy in particular or energy in general. The debt for implementation of any technology, in the sense of making the technology a success in terms of broad acceptance and general utilisation, lies with the particular industry involved. Regardless of the technology, innovation research identifies the implementation phase as most critical to the success of any innovation. In this sense, nuclear technology is at best still an innovation, because the implementation has not yet been completed. Fear and opposition to innovation are ubiquitous. Even the economy - which is often described as 'rational' - is full of this resistance. Innovation has an impact on the pivotal point between stability, the presupposition for the successful execution of decisions already taken and instability, which includes insecurity, but is also necessary for the success of further development. By definition, innovations are beyond our sphere of experience; not at the level of reliability and trust yet to come. Yet they are evaluated via the simplifying heuristics for making decisions proven not only to be necessary and useful, but also accurate in the familiar. The 'settlement of the debt of implementation', the accompanying communication, the decision-making procedures concerning the regulation of averse effects of the technology, but also the tailoring of the new technology or service itself must be directed to appropriate target groups. But the group often aimed at in the nuclear debate, the group, which largely determines political

  9. DNA Structures Generated during Recombination Initiated by Mismatch Repair of Uv-Irradiated Nonreplicating Phage DNA in Escherichia Coli: Requirements for Helicase, Exonucleases, and Recf and Recbcd Functions

    Feng, W. Y.; Hays, J B

    1995-01-01

    During infection of homoimmune Escherichia coli lysogens (``repressed infections''), undamaged non-replicating λ phage DNA circles undergo very little recombination. Prior UV irradiation of phages dramatically elevates recombinant frequencies, even in bacteria deficient in UvrABC-mediated excision repair. We previously reported that 80-90% of this UvrABC-independent recombination required MutHLS function and unmethylated d(GATC) sites, two hallmarks of methyl-directed mismatch repair. We now ...

  10. Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori.

    Mitsudome, Takumi; Mon, Hiroaki; Xu, Jian; Li, Zhiqing; Lee, Jae Man; Patil, Anandrao Ashok; Masuda, Atsushi; Iiyama, Kazuhiro; Morokuma, Daisuke; Kusakabe, Takahiro

    2015-03-01

    DNA methylation is an important epigenetic mechanism involved in gene expression of vertebrates and invertebrates. In general, DNA methylation profile is established by de novo DNA methyltransferases (DNMT-3A, -3B) and maintainance DNA methyltransferase (DNMT-1). DNMT-1 has a strong substrate preference for hemimethylated DNA over the unmethylated one. Because the silkworm genome lacks an apparent homologue of de novo DNMT, it is still unclear that how silkworm chromosome establishes and maintains its DNA methylation profile. As the first step to unravel this enigma, we purified recombinant BmDNMT-1 using baculovirus expression system and characterized its DNA-binding and DNA methylation activity. We found that the BmDNMT-1 preferentially methylates hemimethylated DNA despite binding to both unmethylated and hemimethylated DNA. Interestingly, BmDNMT-1 formed a complex with DNA in the presence or absence of methyl group donor, S-Adenosylmethionine (AdoMet) and the AdoMet-dependent complex formation was facilitated by Zn(2+) and Mn(2+). Our results provide clear evidence that BmDNMT-1 retained the function as maintenance DNMT but its sensitivity to metal ions is different from mammalian DNMT-1. PMID:25623240

  11. DNA methylation detection based on difference of base content

    Sato, Shinobu; Ohtsuka, Keiichi; Honda, Satoshi; Sato, Yusuke; Takenaka, Shigeori

    2016-04-01

    Methylation frequently occurs in cytosines of CpG sites to regulate gene expression. The identification of aberrant methylation of certain genes is important for cancer marker analysis. The aim of this study was to determine the methylation frequency in DNA samples of unknown length and/or concentration. Unmethylated cytosine is known to be converted to thymine following bisulfite treatment and subsequent PCR. For this reason, the AT content in DNA increases with an increasing number of methylation sites. In this study, the fluorescein-carrying bis-acridinyl peptide (FKA) molecule was used for the detection of methylation frequency. FKA contains fluorescein and two acridine moieties, which together allow for the determination of the AT content of double-stranded DNA fragments. Methylated and unmethylated human genomes were subjected to bisulfide treatment and subsequent PCR using primers specific for the CFTR, CDH4, DBC1, and NPY genes. The AT content in the resulting PCR products was estimated by FKA, and AT content estimations were found to be in good agreement with those determined by DNA sequencing. This newly developed method may be useful for determining methylation frequencies of many PCR products by measuring the fluorescence in samples excited at two different wavelengths.

  12. Responsible technology acceptance

    Toft, Madeleine Broman; Schuitema, Geertje; Thøgersen, John

    2014-01-01

    on private consumers’ acceptance of having Smart Grid technology installed in their home. We analyse acceptance in a combined framework of the Technology Acceptance Model and the Norm Activation Model. We propose that individuals are only likely to accept Smart Grid technology if they assess...... usefulness in terms of a positive impact for society and the environment. Therefore, we expect that Smart Grid technology acceptance can be better explained when the well-known technology acceptance parameters included in the Technology Acceptance Model are supplemented by moral norms as suggested by the...... Norm Activation Model. We tested this proposition by means of an online survey of Danish (N=323), Norwegian (N=303) and Swiss (N=324) private consumers. The study confirms that adding personal norms to the independent variables of the Technology Acceptance Model leads to a significant increase in the...

  13. DNA methylation at hepatitis B viral integrants is associated with methylation at flanking human genomic sequences

    Watanabe, Yoshiyuki; Yamamoto, Hiroyuki; Oikawa, Ritsuko; Toyota, Minoru; Yamamoto, Masakazu; Kokudo, Norihiro; Tanaka, Shinji; Arii, Shigeki; Yotsuyanagi, Hiroshi; Koike, Kazuhiko; Itoh, Fumio

    2015-01-01

    Integration of DNA viruses into the human genome plays an important role in various types of tumors, including hepatitis B virus (HBV)–related hepatocellular carcinoma. However, the molecular details and clinical impact of HBV integration on either human or HBV epigenomes are unknown. Here, we show that methylation of the integrated HBV DNA is related to the methylation status of the flanking human genome. We developed a next-generation sequencing-based method for structural methylation analysis of integrated viral genomes (denoted G-NaVI). This method is a novel approach that enables enrichment of viral fragments for sequencing using unique baits based on the sequence of the HBV genome. We detected integrated HBV sequences in the genome of the PLC/PRF/5 cell line and found variable levels of methylation within the integrated HBV genomes. Allele-specific methylation analysis revealed that the HBV genome often became significantly methylated when integrated into highly methylated host sites. After integration into unmethylated human genome regions such as promoters, however, the HBV DNA remains unmethylated and may eventually play an important role in tumorigenesis. The observed dynamic changes in DNA methylation of the host and viral genomes may functionally affect the biological behavior of HBV. These findings may impact public health given that millions of people worldwide are carriers of HBV. We also believe our assay will be a powerful tool to increase our understanding of the various types of DNA virus-associated tumorigenesis. PMID:25653310

  14. Mitochondrial regulation of cancer associated nuclear DNA methylation

    The onset and progression of cancer is associated with the methylation-dependent silencing of specific genes, however, the mechanism and its regulation have not been established. We previously demonstrated that reduction of mitochondrial DNA content induces cancer progression. Here we found that mitochondrial DNA-deficient LNρ0-8 activates the hypermethylation of the nuclear DNA promoters including the promoter CpG islands of the endothelin B receptor, O6-methylguanine-DNA methyltransferase, and E-cadherin. These are unmethylated and the corresponding gene products are expressed in the parental LNCaP containing mitochondrial DNA. The absence of mitochondrial DNA induced DNA methyltransferase 1 expression which was responsible for the methylation patterns observed. Inhibition of DNA methyltransferase eliminated hypermethylation and expressed gene products in LNρ0-8. These studies demonstrate loss or reduction of mitochondrial DNA resulted in the induction of DNA methyltransferase 1, hypermethylation of the promoters of endothelin B receptor, O6-methylguanine-DNA methyltransferase, and E-cadherin, and reduction of the corresponding gene products

  15. ARC Code TI: ACCEPT

    National Aeronautics and Space Administration — ACCEPT consists of an overall software infrastructure framework and two main software components. The software infrastructure framework consists of code written to...

  16. Acceptable noise level

    Olsen, Steen Østergaard; Nielsen, Lars Holme; Lantz, Johannes;

    2012-01-01

    The acceptable noise level (ANL) is used to quantify the amount of background noise that subjects can accept while listening to speech, and is suggested for prediction of individual hearing-aid use. The aim of this study was to assess the repeatability of the ANL measured in normal-hearing subjects...

  17. Newbery Medal Acceptance.

    Freedman, Russell

    1988-01-01

    Presents the Newbery Medal acceptance speech of Russell Freedman, writer of children's nonfiction. Discusses the place of nonfiction in the world of children's literature, the evolution of children's biographies, and the author's work on "Lincoln." (ARH)

  18. Analysis of Scrum acceptance

    Vončina, Bojan

    2016-01-01

    The purpose of the thesis was to analyse the acceptance of Scrum methodology, which has become one of the leading agile methodologies, and to find out which were the key factors that influenced the acceptance. The analysis was conducted in Comtrade, which is one of the largest Slovenian software development companies. The First part (theoretical part) contains an introduction chapter, a detailed presentation of Scrum methodology and the presentation of theoretical models, on which practical ...

  19. Acceptability of human risk.

    Kasperson, R.E.

    1983-01-01

    This paper has three objectives: to explore the nature of the problem implicit in the term "risk acceptability," to examine the possible contributions of scientific information to risk standard-setting, and to argue that societal response is best guided by considerations of process rather than formal methods of analysis. Most technological risks are not accepted but are imposed. There is also little reason to expect consensus among individuals on their tolerance of risk. Moreover, debates abo...

  20. Operations Acceptance Management

    Suchá, Ivana

    2010-01-01

    This paper examines the process of Operations Acceptance Management, whose main task is to control Operations Acceptance Tests (OAT). In the first part the author focuses on the theoretical ground for the problem in the context of ITSM best practices framework ITIL. Benefits, process pitfalls and possibilities for automation are discussed in this part. The second part contains a case study of DHL IT Services (Prague), where a solution optimizing the overall workflow was implemented using simp...

  1. Acceptable noise level

    Olsen, Steen Østergaard; Nielsen, Lars Holme; Lantz, Johannes;

    2012-01-01

    The acceptable noise level (ANL) is used to quantify the amount of background noise that subjects can accept while listening to speech, and is suggested for prediction of individual hearing-aid use. The aim of this study was to assess the repeatability of the ANL measured in normal-hearing subjects...... using running Danish and non-semantic speech materials as stimuli and modulated speech-spectrum and multi-talker babble noises as competing stimuli....

  2. Accepting grammars and systems.

    Bordihn, Henning; Fernau, Henning

    2007-01-01

    We investigate several kinds of regulated rewriting (programmed, matrix, with regular control, ordered, and variants thereof) and of parallel rewriting mechanisms (Lindenmayer systems, uniformly limited Lindenmayer systems, limited Lindenmayer systems and scattered context grammars) as accepting devices, in contrast with the usual generating mode. In some cases, accepting mode turns out to be just as powerful as generating mode, e.g. within the grammars of the Chomsky ...

  3. A combined HM-PCR/SNuPE method for high sensitive detection of rare DNA methylation

    Tierling Sascha

    2010-06-01

    Full Text Available Abstract Background DNA methylation changes are widely used as early molecular markers in cancer detection. Sensitive detection and classification of rare methylation changes in DNA extracted from circulating body fluids or complex tissue samples is crucial for the understanding of tumor etiology, clinical diagnosis and treatment. In this paper, we describe a combined method to monitor the presence of methylated tumor DNA in an excess of unmethylated background DNA of non-tumorous cells. The method combines heavy methyl-PCR, which favors preferential amplification of methylated marker sequence from bisulfite-treated DNA with a methylation-specific single nucleotide primer extension monitored by ion-pair, reversed-phase, high-performance liquid chromatography separation. Results This combined method allows detection of 14 pg (that is, four to five genomic copies of methylated chromosomal DNA in a 2000-fold excess (that is, 50 ng of unmethylated chromosomal background, with an analytical sensitivity of > 90%. We outline a detailed protocol for the combined assay on two examples of known cancer markers (SEPT9 and TMEFF2 and discuss general aspects of assay design and data interpretation. Finally, we provide an application example for rapid testing on tumor methylation in plasma DNA derived from a small cohort of patients with colorectal cancer. Conclusion The method allows unambiguous detection of rare DNA methylation, for example in body fluid or DNA isolates from cells or tissues, with very high sensitivity and accuracy. The application combines standard technologies and can easily be adapted to any target region of interest. It does not require costly reagents and can be used for routine screening of many samples.

  4. Spain accepts safeguards controls

    Spain became the 26th country to accept safeguards controls by the Agency in December. An agreement was signed in Vienna, transferring to IAEA the administration of safeguards against diversion of materials and installations to military purposes provided for under a nuclear co-operation agreement concluded between Spain and USA in 1957

  5. Robustness - acceptance criteria

    Rizzuto, Enrico; Sørensen, John Dalsgaard; Kroon, Inger B.

    2010-01-01

    This factsheet describes the general framework on the bases of which acceptance criteria for requirements on the robustness of structures can be set. Such framework is based on the more general concept of risk-based assessment of engineering systems. The present factsheet is to be seen in...

  6. Approaches to acceptable risk

    Several alternative approaches to address the question open-quotes How safe is safe enough?close quotes are reviewed and an attempt is made to apply the reasoning behind these approaches to the issue of acceptability of radiation exposures received in space. The approaches to the issue of the acceptability of technological risk described here are primarily analytical, and are drawn from examples in the management of environmental health risks. These include risk-based approaches, in which specific quantitative risk targets determine the acceptability of an activity, and cost-benefit and decision analysis, which generally focus on the estimation and evaluation of risks, benefits and costs, in a framework that balances these factors against each other. These analytical methods tend by their quantitative nature to emphasize the magnitude of risks, costs and alternatives, and to downplay other factors, especially those that are not easily expressed in quantitative terms, that affect acceptance or rejection of risk. Such other factors include the issues of risk perceptions and how and by whom risk decisions are made

  7. Approaches to acceptable risk

    Whipple, C.

    1997-04-30

    Several alternative approaches to address the question {open_quotes}How safe is safe enough?{close_quotes} are reviewed and an attempt is made to apply the reasoning behind these approaches to the issue of acceptability of radiation exposures received in space. The approaches to the issue of the acceptability of technological risk described here are primarily analytical, and are drawn from examples in the management of environmental health risks. These include risk-based approaches, in which specific quantitative risk targets determine the acceptability of an activity, and cost-benefit and decision analysis, which generally focus on the estimation and evaluation of risks, benefits and costs, in a framework that balances these factors against each other. These analytical methods tend by their quantitative nature to emphasize the magnitude of risks, costs and alternatives, and to downplay other factors, especially those that are not easily expressed in quantitative terms, that affect acceptance or rejection of risk. Such other factors include the issues of risk perceptions and how and by whom risk decisions are made.

  8. 1984 Newbery Acceptance Speech.

    Cleary, Beverly

    1984-01-01

    This acceptance speech for an award honoring "Dear Mr. Henshaw," a book about feelings of a lonely child of divorce intended for eight-, nine-, and ten-year-olds, highlights children's letters to author. Changes in society that affect children, the inception of "Dear Mr. Henshaw," and children's reactions to books are highlighted. (EJS)

  9. Den betingede accept

    Kolind, Torsten

    1999-01-01

    difficulties in underscoring this normality. This conditioned accept tends to leave the ex-prisoner in an vacuum of signification; he is neither fully normal nor fully deviant. In regard to identity, this vacuum, and the practices that follows, leaves the ex-prisoner badly equipped in his attempt to live up to...

  10. Why was Relativity Accepted?

    Brush, S. G.

    Historians of science have published many studies of the reception of Einstein's special and general theories of relativity. Based on a review of these studies, and my own research on the role of the light-bending prediction in the reception of general relativity, I discuss the role of three kinds of reasons for accepting relativity (1) empirical predictions and explanations; (2) social-psychological factors; and (3) aesthetic-mathematical factors. According to the historical studies, acceptance was a three-stage process. First, a few leading scientists adopted the special theory for aesthetic-mathematical reasons. In the second stage, their enthusiastic advocacy persuaded other scientists to work on the theory and apply it to problems currently of interest in atomic physics. The special theory was accepted by many German physicists by 1910 and had begun to attract some interest in other countries. In the third stage, the confirmation of Einstein's light-bending prediction attracted much public attention and forced all physicists to take the general theory of relativity seriously. In addition to light-bending, the explanation of the advance of Mercury's perihelion was considered strong evidence by theoretical physicists. The American astronomers who conducted successful tests of general relativity became defenders of the theory. There is little evidence that relativity was `socially constructed' but its initial acceptance was facilitated by the prestige and resources of its advocates.

  11. Mitochondrial DNA in the regulation of innate immune responses.

    Fang, Chunju; Wei, Xiawei; Wei, Yuquan

    2016-01-01

    Mitochondrion is known as the energy factory of the cell, which is also a unique mammalian organelle and considered to be evolved from aerobic prokaryotes more than a billion years ago. Mitochondrial DNA, similar to that of its bacterial ancestor’s, consists of a circular loop and contains significant number of unmethylated DNA as CpG islands. The innate immune system plays an important role in the mammalian immune response. Recent research has demonstrated that mitochondrial DNA (mtDNA) activates several innate immune pathways involving TLR9, NLRP3 and STING signaling, which contributes to the signaling platforms and results in effector responses. In addition to facilitating antibacterial immunity and regulating antiviral signaling, mounting evidence suggests that mtDNA contributes to inflammatory diseases following cellular damage and stress. Therefore, in addition to its well-appreciated roles in cellular metabolism and energy production,mtDNA appears to function as a key member in the innate immune system. Here, we highlight the emerging roles of mtDNA in innate immunity. PMID:26498951

  12. Acceptance of Tinnitus : Validation of the Tinnitus Acceptance Questionnaire

    Weise, Cornelia; Kleinstäuber, Maria; Hesser, Hugo; Westin, Vendela; Andersson, Gerhard

    2013-01-01

    The concept of acceptance has recently received growing attention within tinnitus research due to the fact that tinnitus acceptance is one of the major targets of psychotherapeutic treatments. Accordingly, acceptance-based treatments will most likely be increasingly offered to tinnitus patients and assessments of acceptance-related behaviours will thus be needed. The current study investigated the factorial structure of the Tinnitus Acceptance Questionnaire (TAQ) and the role of tinnitus acce...

  13. Waste acceptance and logistics

    There are three major components which are normally highlighted when the Civilian Radioactive Waste Management Program is discussed - the repository, the monitored retrievable storage facility, and the transportation system. These are clearly the major physical system elements and they receive the greatest external attention. However, there will not be a successful, operative waste management system without fully operational waste acceptance plans and logistics arrangements. This paper will discuss the importance of developing, on a parallel basis to the normally considered waste management system elements, the waste acceptance and logistics arrangements to enable the timely transfer of spent nuclear fuel from more than one hundred and twenty waste generators to the Federal government. The paper will also describe the specific activities the Program has underway to make the necessary arrangements. (author)

  14. kitchingroup-57: Accepted

    John Kitchin

    2016-01-01

    This is the accepted version of this manuscript. @article{kitchin-2015-examp, author = {Kitchin, John R.}, title = {Examples of Effective Data Sharing in Scientific Publishing}, journal = {ACS Catalysis}, volume = {5}, number = {6}, pages = {3894-3899}, year = 2015, doi = {10.1021/acscatal.5b00538}, url = { http://dx.doi.org/10.1021/acscatal.5b00538 }, keywords = {DESC0004031, early-career, orgmode, Data sharing }, eprint = { http://dx.doi.org/10.1021/acscatal.5b00538 }, }

  15. Environment and public acceptance

    The problems involved in the siting of nuclear power stations at a local level are of a political economic, social or ecological order. The acceptance of a nuclear station mostly depends on its interest for the local population. In order to avoid negative reactions, the men who are responsible must make the harmonious integration of the station within the existing economic and social context their first priority

  16. Age and Acceptance of Euthanasia.

    Ward, Russell A.

    1980-01-01

    Study explores relationship between age (and sex and race) and acceptance of euthanasia. Women and non-Whites were less accepting because of religiosity. Among older people less acceptance was attributable to their lesser education and greater religiosity. Results suggest that quality of life in old age affects acceptability of euthanasia. (Author)

  17. Collaboration between CpG sites is needed for stable somatic inheritance of DNA methylation states.

    Haerter, Jan O; Lövkvist, Cecilia; Dodd, Ian B; Sneppen, Kim

    2014-02-01

    Inheritance of 5-methyl cytosine modification of CpG (CG/CG) DNA sequences is needed to maintain early developmental decisions in vertebrates. The standard inheritance model treats CpGs as independent, with methylated CpGs maintained by efficient methylation of hemimethylated CpGs produced after DNA replication, and unmethylated CpGs maintained by an absence of de novo methylation. By stochastic simulations of CpG islands over multiple cell cycles and systematic sampling of reaction parameters, we show that the standard model is inconsistent with many experimental observations. In contrast, dynamic collaboration between CpGs can provide strong error-tolerant somatic inheritance of both hypermethylated and hypomethylated states of a cluster of CpGs, reproducing observed stable bimodal methylation patterns. Known recruitment of methylating enzymes by methylated CpGs could provide the necessary collaboration, but we predict that recruitment of demethylating enzymes by unmethylated CpGs strengthens inheritance and allows CpG islands to remain hypomethylated within a sea of hypermethylation. PMID:24288373

  18. Ancient DNA

    Willerslev, Eske; Cooper, Alan

    2004-01-01

    ancient DNA, palaeontology, palaeoecology, archaeology, population genetics, DNA damage and repair......ancient DNA, palaeontology, palaeoecology, archaeology, population genetics, DNA damage and repair...

  19. Acceptance, Tolerance, Participation

    The problem of radioactive waste management from an ethical and societal viewpoint was treated in this seminar, which had participants from universities (social, theological, philosophical and science institutes), waste management industry, and regulatory and controlling authorities. After initial reviews on repository technology, policies and schedules, knowledge gaps, and ethical aspects on decision making under uncertainty, four subjects were treated in lectures and discussions: Democratic collective responsibility, Handling threats in democratic decision making, Waste management - a technological operation with a social dimension, Acceptance and legitimity. Lectures with comments and discussions are collected in this report

  20. Marketing for Acceptance

    Tina L. Johnston, Ph.D.

    2009-11-01

    Full Text Available Becoming a researcher comes with the credentializing pressure to publish articles in peer-reviewed journals (Glaser, 1992; Glaser, 2007; Glaser, 2008. The work intensive process is exacerbated when the author’s research method is grounded theory. This study investigated the concerns of early and experienced grounded theorists to discover how they worked towards publishing research projects that applied grounded theory as a methodology. The result was a grounded theory of marketing for acceptance that provides the reader with insight into ways that classic grounded theorists have published their works. This is followed by a discussion of ideas for normalizing classic grounded theory research methods in our substantive fields.

  1. High acceptance recoil polarimeter

    In order to detect neutrons and protons in the 50 to 600 MeV energy range and measure their polarization, an efficient, low-noise, self-calibrating device is being designed. This detector, known as the High Acceptance Recoil Polarimeter (HARP), is based on the recoil principle of proton detection from np→n'p' or pp→p'p' scattering (detected particles are underlined) which intrinsically yields polarization information on the incoming particle. HARP will be commissioned to carry out experiments in 1994

  2. Morsleben waste acceptance requirements

    The Morsleben waste acceptance requirements take into account the valid stipulations in the permanent operational license of the Morsleben repository for radioactive waste (Eram) dated April 22, 1986, and in the documents underlying it. In line with the guaranteed continued validity until June 30, 2000 of this permanent operational license, also the classification of radioactive waste by waste types (A) and radiation protection groups (S) was retained. The revised requirements to be met by radioactive waste to be disposed of in Eram as issued by the German Federal Office for Radiation Protection (BfS) effective August 1996 constitute the safety framework which must be adhered to by this waste. They also take into account other stipulations by the BfS, especially voluntary restrictions and conditions imposed by the self-surveillance. Eram accepts the 'solid waste' and 'spent sealed radiation sources' categories. The waste generators must observe the requirements to be met by waste to be stored permanently. In the case of solid waste, these conditions include criteria to be met by waste forms, activity limitations for radionuclides and groups of radionuclides, and packaging criteria. (orig.)

  3. Is nuclear power acceptable

    The energy shortage forecast for the early 21st century is considered. Possible energy sources other than fossil fuel are stated as geothermal, fusion, solar and fission, of which only fission has been demonstrated technically and economically. The environmental impacts of fission are examined. The hazards are discussed under the following headings: nuclear accident, fatality risk, safe reactor, property damage, acts of God, low-level release of radioactivity, diversion of fissile material and sabotage, radioactive waste disposal, toxicity of plutonium. The public reaction to nuclear power is analyzed, and proposals are made for a programme of safety and security which the author hopes will make it acceptable as the ultimate energy source. (U.K.)

  4. DNA media storage

    Christy M.Bogard; Eric C.Rouchka; Benjamin Arazi

    2008-01-01

    In 1994. University of Southern California computer scientist,Dr.Leonard Adleman solved the Hamiltonian path problem using DNA as a computational mechanism.He proved the principle that DNA computing could be used to solve computationally complex problems.Because of the limitations in discovery time,resource requirements,and sequence mismatches,DNA computing has not yet become a commonly accepted practice.However,advancements are continually being discovered that are evolving the field of DNA computing.Practical applications of DNA are not restricted to computation alone.This research presents a novel approach in which DNA could be used as a means of storing files.Through the use of multiple sequence alignment combined with intelligent heuristics,the most probabilistic file contents can be determined with minimal errors.

  5. Public acceptance of nuclear power

    The lecture addresses the question why we need public acceptance work and provides some clues to it. It explains various human behaviour patterns which determine the basics for public acceptance. To some extent, the opposition to nuclear energy and the role the media play are described. Public acceptance efforts of industry are critically reviewed. Some hints on difficulties with polling are provided. The lecture concludes with recommendations for further public acceptance work. (author)

  6. DNA methylation patterns in cord blood DNA and body size in childhood.

    Caroline L Relton

    Full Text Available Epigenetic markings acquired in early life may have phenotypic consequences later in development through their role in transcriptional regulation with relevance to the developmental origins of diseases including obesity. The goal of this study was to investigate whether DNA methylation levels at birth are associated with body size later in childhood.A study design involving two birth cohorts was used to conduct transcription profiling followed by DNA methylation analysis in peripheral blood. Gene expression analysis was undertaken in 24 individuals whose biological samples and clinical data were collected at a mean ± standard deviation (SD age of 12.35 (0.95 years, the upper and lower tertiles of body mass index (BMI were compared with a mean (SD BMI difference of 9.86 (2.37 kg/m(2. This generated a panel of differentially expressed genes for DNA methylation analysis which was then undertaken in cord blood DNA in 178 individuals with body composition data prospectively collected at a mean (SD age of 9.83 (0.23 years. Twenty-nine differentially expressed genes (>1.2-fold and p<10(-4 were analysed to determine DNA methylation levels at 1-3 sites per gene. Five genes were unmethylated and DNA methylation in the remaining 24 genes was analysed using linear regression with bootstrapping. Methylation in 9 of the 24 (37.5% genes studied was associated with at least one index of body composition (BMI, fat mass, lean mass, height at age 9 years, although only one of these associations remained after correction for multiple testing (ALPL with height, p(Corrected = 0.017.DNA methylation patterns in cord blood show some association with altered gene expression, body size and composition in childhood. The observed relationship is correlative and despite suggestion of a mechanistic epigenetic link between in utero life and later phenotype, further investigation is required to establish causality.

  7. Promoter methylation analysis of O6-methylguanine-DNA methyltransferase in glioblastoma: detection by locked nucleic acid based quantitative PCR using an imprinted gene (SNURF as a reference

    Pession Annalisa

    2010-02-01

    Full Text Available Abstract Background Epigenetic silencing of the MGMT gene by promoter methylation is associated with loss of MGMT expression, diminished DNA-repair activity and longer overall survival in patients with glioblastoma who, in addition to radiotherapy, received alkylating chemotherapy with carmustine or temozolomide. We describe and validate a rapid methylation sensitive quantitative PCR assay (MS-qLNAPCR using Locked Nucleic Acid (LNA modified primers and an imprinted gene as a reference. Methods An analysis was made of a database of 159 GBM patients followed between April 2004 and October 2008. After bisulfite treatment, methylated and unmethylated CpGs were recognized by LNA primers and molecular beacon probes. The SNURF promoter of an imprinted gene mapped on 15q12, was used as a reference. This approach was used because imprinted genes have a balanced copy number of methylated and unmethylated alleles, and this feature allows an easy and a precise normalization. Results Concordance between already described nested MS-PCR and MS-qLNAPCR was found in 158 of 159 samples (99.4%. The MS-qLNAPCR assay showed a PCR efficiency of 102% and a sensitivity of 0.01% for LNA modified primers, while unmodified primers revealed lower efficiency (69% and lower sensitivity (0.1%. MGMT promoter was found to be methylated using MS-qLNAPCR in 70 patients (44.02%, and completely unmethylated in 89 samples (55.97%. Median overall survival was of 24 months, being 20 months and 36 months, in patients with MGMT unmethylated and methylated, respectively. Considering MGMT methylation data provided by MS-qLNAPCR as a binary variable, overall survival was different between patients with GBM samples harboring MGMT promoter unmethylated and other patients with any percentage of MGMT methylation (p = 0.003. This difference was retained using other cut off values for MGMT methylation rate (i.e. 10% and 20% of methylated allele, while the difference was lost when 50% of MGMT

  8. Public acceptance of biofuels

    The public acceptance of biofuels in Greece is examined in this work. The analysis of 571 face to face interviews shows that 90.7% of the respondents believe that climatic changes are related to fossil fuel consumption, while only 23.8% know the difference between biodiesel and bioethanol. 76.1% believe that energy saving should precede the use of an alternative source of energy. Only 27.3% believe that priority must be given to biofuels over other renewable energy sources. Only 49.9% think that the use of biofuels can be an effective solution against climatic changes and 53.9% believe that the use of biofuels can be an effective solution for the energy problem. Finally, 80.9% of the car owners are willing to use biofuels, 44.8% are willing to pay the supplementary amount of 0.06 Euro /L of the fuel market price, while the average amount reported as willing to pay was 0.079 Euro /L on top of the fuel market price. Furthermore, eight models correlating the eight main responses with several socioeconomic variables are developed and analyzed. Those findings heave important policy implications related to the use and promotion of biofuels.

  9. Public acceptance of biofuels

    Savvanidou, Electra; Zervas, Efthimios; Tsagarakis, Konstantinos P. [Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100 Xanthi (Greece)

    2010-07-15

    The public acceptance of biofuels in Greece is examined in this work. The analysis of 571 face to face interviews shows that 90.7% of the respondents believe that climatic changes are related to fossil fuel consumption, while only 23.8% know the difference between biodiesel and bioethanol. 76.1% believe that energy saving should precede the use of an alternative source of energy. Only 27.3% believe that priority must be given to biofuels over other renewable energy sources. Only 49.9% think that the use of biofuels can be an effective solution against climatic changes and 53.9% believe that the use of biofuels can be an effective solution for the energy problem. Finally, 80.9% of the car owners are willing to use biofuels, 44.8% are willing to pay the supplementary amount of 0.06 EUR/L of the fuel market price, while the average amount reported as willing to pay was 0.079 EUR/L on top of the fuel market price. Furthermore, eight models correlating the eight main responses with several socioeconomic variables are developed and analyzed. Those findings heave important policy implications related to the use and promotion of biofuels. (author)

  10. Escherichia coli OxyR modulation of bacteriophage Mu mom expression in dam+ cells can be attributed to its ability to bind hemimethylated Pmom promoter DNA.

    Hattman, S; Sun, W.

    1997-01-01

    Transcription of the bacteriophage Mu mom operon is strongly repressed by the host OxyR protein in dam - but not dam + cells. In this work we show that the extent of mom modification is sensitive to the relative levels of the Dam and OxyR proteins and OxyR appears to modulate the level of mom expression even in dam + cells. In vitro studies demonstrated that OxyR is capable of binding hemimethylated P mom , although its affinity is reduced slightly compared with unmethylated DNA. Thus, OxyR m...

  11. Role of DNA profiling in forensic odontology

    S Leena Sakari; Sudha Jimson; K M K Masthan; Jenita Jacobina

    2015-01-01

    The recent advances in DNA profiling have made DNA evidence to be more widely accepted in courts. This has revolutionized the aspect of forensic odontology. DNA profiling/DNA fingerprinting has come a long way from the conventional fingerprints. DNA that is responsible for all the cell′s activities, yields valuable information both in the healthy and diseased individuals. When other means of traditional identification become impossible following mass calamities or fire explosions, teeth provi...

  12. Quantitation of DNA methylation by melt curve analysis

    Jones Michael E

    2009-04-01

    Full Text Available Abstract Background Methylation of DNA is a common mechanism for silencing genes, and aberrant methylation is increasingly being implicated in many diseases such as cancer. There is a need for robust, inexpensive methods to quantitate methylation across a region containing a number of CpGs. We describe and validate a rapid, in-tube method to quantitate DNA methylation using the melt data obtained following amplification of bisulfite modified DNA in a real-time thermocycler. Methods We first describe a mathematical method to normalise the raw fluorescence data generated by heating the amplified bisulfite modified DNA. From this normalised data the temperatures at which melting begins and finishes can be calculated, which reflect the less and more methylated template molecules present respectively. Also the T50, the temperature at which half the amplicons are melted, which represents the summative methylation of all the CpGs in the template mixture, can be calculated. These parameters describe the methylation characteristics of the region amplified in the original sample. Results For validation we used synthesized oligonucleotides and DNA from fresh cells and formalin fixed paraffin embedded tissue, each with known methylation. Using our quantitation we could distinguish between unmethylated, partially methylated and fully methylated oligonucleotides mixed in varying ratios. There was a linear relationship between T50 and the dilution of methylated into unmethylated DNA. We could quantitate the change in methylation over time in cell lines treated with the demethylating drug 5-aza-2'-deoxycytidine, and the differences in methylation associated with complete, clonal or no loss of MGMT expression in formalin fixed paraffin embedded tissues. Conclusion We have validated a rapid, simple in-tube method to quantify methylation which is robust and reproducible, utilizes easily designed primers and does not need proprietary algorithms or software. The

  13. Direct and site-specific quantification of RNA 2'-O-methylation by PCR with an engineered DNA polymerase.

    Aschenbrenner, Joos; Marx, Andreas

    2016-05-01

    Methylation of the 2'-hydroxyl-group of ribonucleotides is found in all major classes of RNA in eukaryotes and is one of the most abundant posttranscriptional modifications of stable RNAs. In spite of intense studies, the multiple functions of RNA 2'-O-methylation are still not understood. One major obstacle in the field are the technical demanding detection methods, which are typically laborious and do not always deliver unambiguous results. We present a thermostable KlenTaq DNA polymerase variant with significant reverse transcription activity that is able to discriminate 2'-O-methylated from unmethylated RNAs. The engineered enzyme catalyzes DNA synthesis from DNA as well as RNA templates and enables expeditious quantification of 2'-O-methylation of individual nucleotides directly from total RNA extracts by a simple qRT-PCR. PMID:27016740

  14. Replication independent DNA double-strand break retention may prevent genomic instability

    Pornthanakasem Wichai

    2010-03-01

    Full Text Available Abstract Background Global hypomethylation and genomic instability are cardinal features of cancers. Recently, we established a method for the detection of DNA methylation levels at sites close to endogenous DNA double strand breaks (EDSBs, and found that those sites have a higher level of methylation than the rest of the genome. Interestingly, the most significant differences between EDSBs and genomes were observed when cells were cultured in the absence of serum. DNA methylation levels on each genomic location are different. Therefore, there are more replication-independent EDSBs (RIND-EDSBs located in methylated genomic regions. Moreover, methylated and unmethylated RIND-EDSBs are differentially processed. Euchromatins respond rapidly to DSBs induced by irradiation with the phosphorylation of H2AX, γ-H2AX, and these initiate the DSB repair process. During G0, most DSBs are repaired by non-homologous end-joining repair (NHEJ, mediated by at least two distinct pathways; the Ku-mediated and the ataxia telangiectasia-mutated (ATM-mediated. The ATM-mediated pathway is more precise. Here we explored how cells process methylated RIND-EDSBs and if RIND-EDSBs play a role in global hypomethylation-induced genomic instability. Results We observed a significant number of methylated RIND-EDSBs that are retained within deacetylated chromatin and free from an immediate cellular response to DSBs, the γ-H2AX. When cells were treated with tricostatin A (TSA and the histones became hyperacetylated, the amount of γ-H2AX-bound DNA increased and the retained RIND-EDSBs were rapidly repaired. When NHEJ was simultaneously inhibited in TSA-treated cells, more EDSBs were detected. Without TSA, a sporadic increase in unmethylated RIND-EDSBs could be observed when Ku-mediated NHEJ was inhibited. Finally, a remarkable increase in RIND-EDSB methylation levels was observed when cells were depleted of ATM, but not of Ku86 and RAD51. Conclusions Methylated RIND-EDSBs are

  15. American acceptance of nuclear power

    The characteristic adventurous spirit that built American technology will eventually lead to American acceptance of nuclear power unless an overpowering loss of nerve causes us to reject both nuclear technology and world leadership. The acceptance of new technology by society has always been accompanied by activist opposition to industralization. To resolve the debate between environmental and exploitive extremists, we must accept with humility the basic premise that human accomplishment is a finite part of nature

  16. American acceptance of nuclear power

    Barrett, W.

    The characteristic adventurous spirit that built American technology will eventually lead to American acceptance of nuclear power unless an overpowering loss of nerve causes us to reject both nuclear technology and world leadership. The acceptance of new technology by society has always been accompanied by activist opposition to industralization. To resolve the debate between environmental and exploitive extremists, we must accept with humility the basic premise that human accomplishment is a finite part of nature. (DCK)

  17. Decision modeling and acceptance criteria

    Ditlevsen, Ove Dalager

    2003-01-01

    formulation of decision criteria and public acceptance criteria connected to risk analysis of technical operations that may endanger human life and property. Public restrictions on the decisions concerning the design, construction and managing of the technical operation have in the past been imposed on the......, Ronold K. Societal indicators and risk acceptance. In: 17th International Conference on Offshote Mechanics and Arctic Engineering, number OMAE98-1488. ASME; 1998; Rackwitz R. Optimization and risk acceptability based on the Life Quality Index. Structural Safety 2002;24;297-331.). Keywords: Acceptance...

  18. Radioactive waste package acceptance criteria

    Preliminary acceptance criteria have been developed for packages containing nuclear waste which must be stored or disposed of by the US Department of Energy. Acceptance criteria are necessary to ensure that the waste packages are compatible with all elements of the Waste Management System. The acceptance criteria are subject to revision since many of the constraints that will be imposed on the waste packages by the Waste Management System have either not been defined or are being revised. Delineation of the acceptance criteria will provide bases for handling, transporting and disposing of the commercial waste

  19. Cell-free fetal DNA and cell-free total DNA levels in spontaneous abortion with fetal chromosomal aneuploidy.

    Ji Hyae Lim

    Full Text Available BACKGROUND: Cell-free fetal DNA and cell-free total DNA in maternal circulation have been proposed as potential markers for noninvasive monitoring of the placental condition during the pregnancy. However, the correlation of and change in cell-free fetal DNA and cell-free total DNA in spontaneous abortion (SA with fetal chromosomal aneuploidy have not yet been reported. Therefore, we investigated cell-free fetal DNA and cell-free total DNA levels in SA women with fetal chromosomal aneuploidy. METHODOLOGY/PRINCIPAL FINDINGS: A nested case-control study was conducted with maternal plasma collected from 268 women in their first trimester of pregnancy. Subjects included 41 SA with normal fetal karyotype, 26 SA with fetal chromosomal aneuploidy, and 201 normal controls. The unmethylated PDE9A gene was used to measure the maternal plasma levels of cell-free fetal DNA. The GAPDH gene was used to measure the maternal plasma levels of cell-free total DNA. The diagnostic accuracy was measured using receiver-operating characteristic (ROC curves. Levels of cell-free fetal DNA and cell-free total DNA were significantly higher in both SA women with normal fetal karyotype and SA women with fetal chromosomal aneuploidy in comparison with the normal controls (P<0.001 in both. The correlation between cell-free fetal DNA and cell-free total DNA levels was stronger in the normal controls (r = 0.843, P<0.001 than in SA women with normal karyotype (r = 0.465, P = 0.002 and SA women with fetal chromosomal aneuploidy (r = 0.412, P = 0.037. The area under the ROC curve for cell-free fetal DNA and cell-free total DNA was 0.898 (95% CI, 0.852-0.945 and 0.939 (95% CI, 0.903-0.975, respectively. CONCLUSIONS: Significantly high levels of cell-free fetal DNA and cell-free total DNA were found in SA women with fetal chromosomal aneuploidy. Our findings suggest that cell-free fetal DNA and cell-free total DNA may be useful biomarkers for the prediction of SA

  20. Altered DNA methylation patterns of the H19 differentially methylated region and the DAZL gene promoter are associated with defective human sperm.

    Bo Li

    Full Text Available DNA methylation disturbance is associated with defective human sperm. However, oligozoospermia (OZ and asthenozoospermia (AZ usually present together, and the relationship between the single-phenotype defects in human sperm and DNA methylation is poorly understood. In this study, 20 infertile OZ patients and 20 infertile AZ patients were compared with 20 fertile normozoospermic men. Bisulfate-specific PCR was used to analyze DNA methylation of the H19-DMR and the DAZL promoter in these subjects. A similar DNA methylation pattern of the H19-DMR was detected in AZ and NZ(control, with only complete methylation and mild hypomethylation(0.05. However, the methylation pattern of severe hypomethylation (>50% unmethylated CpGs and complete unmethylation was only detected in 5 OZ patients, and the occurrence of these two methylation patterns was 8.54±10.86% and 9±6.06%, respectively. Loss of DNA methylation of the H19-DMR in the OZ patients was found to mainly occur in CTCF-binding site 6, with occurrence of 18.15±14.71%, which was much higher than that in patients with NZ (0.84±2.05% and AZ (0.58±1.77% (P20% methylated clones in the DAZL promoter only in infertile patients, there was no significant difference between the AZ and OZ patients in the proportion of moderately-to-severely hypermethylated clones (p>0.05. In all cases, global sperm genome methylation analyses, using LINE1 transposon as the indicator, showed that dysregulation of DNA methylation is specifically associated with the H19-DMR and DAZL promoter. Therefore, abnormal DNA methylation status of H19-DMR, especially at the CTCF-binding site 6, is closely associated with OZ. Abnormal DNA methylation of the DAZL promoter might represent an epigenetic marker of male infertility.

  1. Worldwide nuclear revival and acceptance

    The current status and trends of the nuclear revival in Europe and abroad are outlined. The development of public opinion in the last decade is playing an important part. This has turned from clear rejection to careful acceptance. Transparency and open communication will be important aspects in the further development of nuclear acceptance. (orig.)

  2. Aberrant DNA methylation in 5'regions of DNA methyltransferase genes in aborted bovine clones

    2008-01-01

    High rate of abortion and developmental abnormalities is thought to be closely associated with inefficient epigenetic reprogramming of the transplanted nuclei during bovine cloning.It is known that one of the important mechanisms for epigenetic reprogramming is DNA methylation.DNA methylation is established and maintained by DNA methyltransferases(DNMTs),therefore,it is postulated that the inefficient epigenetic reprogramming of transplanted nuclei may be due to abnormal expression of DNMTs.Since DNA methylation can strongly inhibit gene expression,aberrant DNA methylation of DNMT genes may disturb gene expression.But presently,it is not clear whether the methylation abnormality of DNMT genes is related to developmental failure of somatic cell nuclear transfer embryos.In our study,we analyzed methylation patterns of the 5' regions of four DNMT genes including Dnmt3a,Dnmt3b,Dnmtl and Dnmt2 in four aborted bovine clones.Using bisulfite sequencing method,we found that 3 out of 4 aborted bovine clones(AF1,AF2 and AF3)showed either hypermethylation or hypomethylation in the 5' regions of Dnmt3a and Dnmt3b.indicating that Dnmt3a and Dnmt3b genes are not properly reprogrammed.However,the individual AF4 exhibited similar methylation level and pattern to age-matched in vitro fertilized (IVF)fetuses.Besides,we found that tle 5'regions of Dnmtl and Dnmt2 were nearly completely unmethylated in all normal adults.IVF fetuses,sperm and aborted clones.Together,our results suggest that the aberrant methylation of Dnmt3a and Dnmt3b 5' regions is probably associated with the high abortion of bovine clones.

  3. Mobile small RNAs regulate genome-wide DNA methylation.

    Lewsey, Mathew G; Hardcastle, Thomas J; Melnyk, Charles W; Molnar, Attila; Valli, Adrián; Urich, Mark A; Nery, Joseph R; Baulcombe, David C; Ecker, Joseph R

    2016-02-01

    RNA silencing at the transcriptional and posttranscriptional levels regulates endogenous gene expression, controls invading transposable elements (TEs), and protects the cell against viruses. Key components of the mechanism are small RNAs (sRNAs) of 21-24 nt that guide the silencing machinery to their nucleic acid targets in a nucleotide sequence-specific manner. Transcriptional gene silencing is associated with 24-nt sRNAs and RNA-directed DNA methylation (RdDM) at cytosine residues in three DNA sequence contexts (CG, CHG, and CHH). We previously demonstrated that 24-nt sRNAs are mobile from shoot to root in Arabidopsis thaliana and confirmed that they mediate DNA methylation at three sites in recipient cells. In this study, we extend this finding by demonstrating that RdDM of thousands of loci in root tissues is dependent upon mobile sRNAs from the shoot and that mobile sRNA-dependent DNA methylation occurs predominantly in non-CG contexts. Mobile sRNA-dependent non-CG methylation is largely dependent on the DOMAINS REARRANGED METHYLTRANSFERASES 1/2 (DRM1/DRM2) RdDM pathway but is independent of the CHROMOMETHYLASE (CMT)2/3 DNA methyltransferases. Specific superfamilies of TEs, including those typically found in gene-rich euchromatic regions, lose DNA methylation in a mutant lacking 22- to 24-nt sRNAs (dicer-like 2, 3, 4 triple mutant). Transcriptome analyses identified a small number of genes whose expression in roots is associated with mobile sRNAs and connected to DNA methylation directly or indirectly. Finally, we demonstrate that sRNAs from shoots of one accession move across a graft union and target DNA methylation de novo at normally unmethylated sites in the genomes of root cells from a different accession. PMID:26787884

  4. DNA Book

    Kawai, Jun; Hayashizaki, Yoshihide

    2003-01-01

    We propose herein a new method of DNA distribution, whereby DNA clones or PCR products are printed directly onto the pages of books and delivered to users along with relevant scientific information. DNA sheets, comprising water-soluble paper onto which DNA is spotted, can be bound into books. Readers can easily extract the DNA fragments from DNA sheets and amplify them using PCR. We show that DNA sheets can withstand various conditions that may be experienced during bookbinding and deli...

  5. Cleaving DNA with DNA

    Carmi, Nir; Balkhi, Shameelah R.; Breaker, Ronald R.

    1998-03-01

    A DNA structure is described that can cleave single-stranded DNA oligonucleotides in the presence of ionic copper. This ``deoxyribozyme'' can self-cleave or can operate as a bimolecular complex that simultaneously makes use of duplex and triplex interactions to bind and cleave separate DNA substrates. Bimolecular deoxyribozyme-mediated strand scission proceeds with a kobs of 0.2 min-1, whereas the corresponding uncatalyzed reaction could not be detected. The duplex and triplex recognition domains can be altered, making possible the targeted cleavage of single-stranded DNAs with different nucleotide sequences. Several small synthetic DNAs were made to function as simple ``restriction enzymes'' for the site-specific cleavage of single-stranded DNA.

  6. L-286, Acceptance Test Record

    This document provides a detailed account of how the acceptance testing was conducted for Project L-286, ''200E Area Sanitary Water Plant Effluent Stream Reduction''. The testing of the L-286 instrumentation system was conducted under the direct supervision

  7. Public acceptance of small reactors

    The success of any nuclear program requires acceptance by the local public and all levels of government involved in the decision to initiate a reactor program. Public acceptance of a nuclear energy source is a major challenge in successful initiation of a small reactor program. In AECL's experience, public acceptance will not be obtained until the public is convinced that the specific nuclear program is needed, safe and economic and environmental benefit to the community. The title of public acceptance is misleading. The objective of the program is a fully informed public. The program proponent cannot force public acceptance, which is beyond his control. He can, however, ensure that the public is informed. Once information has begun to flow to the public by various means as will be explained later, the proponent is responsible to ensure that the information that is provided by him and by others is accurate. Most importantly, and perhaps most difficult to accomplish, the proponent must develop a consultative process that allows the proponent and the public to agree on actions that are acceptable to the proponent and the community

  8. Nucleotide discrimination with DNA immobilized in the MspA nanopore.

    Manrao, Elizabeth A; Derrington, Ian M; Pavlenok, Mikhail; Niederweis, Michael; Gundlach, Jens H

    2011-01-01

    Nanopore sequencing has the potential to become a fast and low-cost DNA sequencing platform. An ionic current passing through a small pore would directly map the sequence of single stranded DNA (ssDNA) driven through the constriction. The pore protein, MspA, derived from Mycobacterium smegmatis, has a short and narrow channel constriction ideally suited for nanopore sequencing. To study MspA's ability to resolve nucleotides, we held ssDNA within the pore using a biotin-NeutrAvidin complex. We show that homopolymers of adenine, cytosine, thymine, and guanine in MspA exhibit much larger current differences than in α-hemolysin. Additionally, methylated cytosine is distinguishable from unmethylated cytosine. We establish that single nucleotide substitutions within homopolymer ssDNA can be detected when held in MspA's constriction. Using genomic single nucleotide polymorphisms, we demonstrate that single nucleotides within random DNA can be identified. Our results indicate that MspA has high signal-to-noise ratio and the single nucleotide sensitivity desired for nanopore sequencing devices. PMID:21991340

  9. Label free colorimetric and fluorimetric direct detection of methylated DNA based on silver nanoclusters for cancer early diagnosis.

    Dadmehr, Mehdi; Hosseini, Morteza; Hosseinkhani, Saman; Ganjali, Mohammad Reza; Sheikhnejad, Reza

    2015-11-15

    Epigenetic changes such as DNA methylation of CpG islands located in the promoter region of some tumor suppressor genes are very common in human diseases such as cancer. Detection of aberrant methylation pattern could serve as an excellent diagnostic approach. Recently, the direct detection of methylated DNA sequences without using chemical and enzymatic treatments or antibodies has received great deal of attentions. In this study, we report a colorimetric and fluorimetric technique for direct detection of DNA methylation. Here, the DNA is being used as an effective template for fluorescent silver nanoclusters formation without any chemical modification or DNA labeling. The sensitivity test showed that upon the addition of target methylated DNA, the fluorescence intensity is decreased in a linear range when the concentration of methylated DNA has increased from 2.0×10(-9) to 6.3 ×10(-7) M with the detection limit of 9.4×10(-10) M. The optical and fluorescence spectral behaviors were highly reproducible and clearly discriminated between unmethylated, methylated and even partially methylated DNA in CpG rich sequences. The results were also reproducible when the human plasma was present in our assay system. PMID:26056954

  10. O6-methylguanine-DNA methyltransferase activity is associated with response to alkylating agent therapy and with MGMT promoter methylation in glioblastoma and anaplastic glioma

    Bobola, Michael S.; Alnoor, Mohammad; Chen, John Y.-S.; Kolstoe, Douglas D.; Silbergeld, Daniel L.; Rostomily, Robert C.; Blank, A.; Chamberlain, Marc C.; Silber, John R.

    2014-01-01

    Background CpG methylation in the O6-methylguanine-DNA methyltransferase (MGMT) promoter is associated with better outcome following alkylating agent chemotherapy in glioblastoma (GBM) and anaplastic glioma (AG). To what extent improved response reflects low or absent MGMT activity in glioma tissue has not been unequivocally assessed. This information is central to developing anti-resistance therapies. Methods We examined the relationship of MGMT activity in 91 GBMs and 84 AGs with progression-free survival (PFS) following alkylator therapy and with promoter methylation status determined by methylation-specific PCR (MSP). Results Cox regression analysis revealed that GBMs with high activity had a significantly greater risk for progression in dichotomous (P ≤ 0.001) and continuous (P ≤ 0.003) models, an association observed for different alkylator regimens, including concurrent chemo-radiation with temozolomide. Analysis of MGMT promoter methylation status in 47 of the GBMs revealed that methylated tumors had significantly lower activity (P ≤ 0.005) and longer PFS (P ≤ 0.036) compared to unmethylated tumors, despite overlapping activities. PFS was also significantly greater in methylated vs. unmethylated GBMs with comparable activity (P ≤ 0.005), and among unmethylated tumors with less than median activity (P ≤ 0.026), suggesting that mechanisms in addition to MGMT promote alkylator resistance. Similar associations of MGMT activity with PFS and promoter methylation status were observed for AGs. Conclusions Our results provide strong support for the hypotheses that MGMT activity promotes alkylator resistance and reflects promoter methylation status in malignant gliomas. General significance MGMT activity is an attractive target for anti-resistance therapy regardless of methylation status. PMID:25558448

  11. Wind power: basic challenge concerning social acceptance

    M. Wolsink

    2012-01-01

    This reference article gives an overview of social acceptance (acceptance by all relevant actors in society) of all relevant aspects of implementation and diffusion of wind power. In social acceptance three dimensions of acceptance are distinguished (socio-political -; community -; market acceptance

  12. The real problem: public acceptability

    The paper concerns the problem of public acceptibility of radioactive waste disposal in the United Kingdom. U.K. Nirex has responsibility for developing a deep repository for low-and intermediate-level radioactive wastes in the U.K., and it is also developing a public information programme aimed at obtaining public acceptibility for such a repository. The main objective of the programme is to raise the level of public awareness of radioactive waste disposal to the point where scientifically supported assurances on safety and environmental impact would be accepted by concerned people. Previous experience of Nirex in dealing with the public on the waste disposal issue is described - this includes the public opposition to the proposed use of Billingham and Elstow as sites for a repository. The main lesson learnt from the latter opposition by Nirex is that public relations must be part of Nirex's main business. (U.K.)

  13. Stochastic phonological grammars and acceptability

    Coleman, J; Coleman, John; Pierrehumbert, Janet

    1997-01-01

    In foundational works of generative phonology it is claimed that subjects can reliably discriminate between possible but non-occurring words and words that could not be English. In this paper we examine the use of a probabilistic phonological parser for words to model experimentally-obtained judgements of the acceptability of a set of nonsense words. We compared various methods of scoring the goodness of the parse as a predictor of acceptability. We found that the probability of the worst part is not the best score of acceptability, indicating that classical generative phonology and Optimality Theory miss an important fact, as these approaches do not recognise a mechanism by which the frequency of well-formed parts may ameliorate the unacceptability of low-frequency parts. We argue that probabilistic generative grammars are demonstrably a more psychologically realistic model of phonological competence than standard generative phonology or Optimality Theory.

  14. Nuclear Energy and Public Acceptance

    The continued use of nuclear power in the European Union and elsewhere requires an adequate level of public and political acceptance. A lack of acceptance is often mistakenly cited as a reason for the slowdown in nuclear power plant construction in Western Europe and as a justification for abandoning nuclear power. In fact, the reasons for the slowdown have more to do with the following two factors: Plentiful supplies of low-priced natural gas, making gas-fired power plants a more attractive investment choice; more than adequate supplies of electricity which have curbed the need for the construction of new plant of any kind. In general, moves towards a withdrawal from nuclear in certain Community countries have been due to party political pressures and have not been a response to public opposition to nuclear. In addition, opinion polls do not show widespread public opposition to the use of nuclear power. Figures consistently indicate that the use of nuclear power does not come high on the list of most people's main worries. Their main concerns focus on other issues such as crime and financial problems. In the main, electricity is taken for granted in the industrialised world. Electric power only becomes an issue when there is a threat of shortages. So if public acceptance is not the main obstacle, what is? Political acceptance is an integral part of the process in which nuclear becomes acceptable or not. The relationship between public and political acceptance and the role of the industry in this context, on how to foster a better trialogue, will be examined. (author)

  15. Acceptance test of LANTIS system

    Acceptance tests of radiotherapy equipment are very important. It is essential for the quality control of the equipment. Quality assurance checks for LANTIS was done during the installation and also periodically. QA involves checks for individual workstations, as well as for overall system. In modern radiotherapy centers, transferring data from one workstation to another via networking improves the quality of the treatment, reduces the errors and reduces the overall treatment time. In this hospital, Local Area Network Therapy Information system (LANTIS) from Siemens, is available for patient data transfer. Here, some of the acceptance checks carried out are presented

  16. DNA supercoiling inhibits DNA knotting.

    Burnier Y.; Dorier J.; Stasiak A.

    2008-01-01

    Despite the fact that in living cells DNA molecules are long and highly crowded, they are rarely knotted. DNA knotting interferes with the normal functioning of the DNA and, therefore, molecular mechanisms evolved that maintain the knotting and catenation level below that which would be achieved if the DNA segments could pass randomly through each other. Biochemical experiments with torsionally relaxed DNA demonstrated earlier that type II DNA topoisomerases that permit inter- and intramolecu...

  17. Intra-Peritoneal Administration of Mitochondrial DNA Provokes Acute Lung Injury and Systemic Inflammation via Toll-Like Receptor 9.

    Zhang, Lemeng; Deng, Songyun; Zhao, Shuangping; Ai, Yuhang; Zhang, Lina; Pan, Pinhua; Su, Xiaoli; Tan, Hongyi; Wu, Dongdong

    2016-01-01

    The pathogenesis of sepsis is complex. Mitochondrial dysfunction, which is responsible for energy metabolism, intrinsic apoptotic pathway, oxidative stress, and systemic inflammatory responses, is closely related with severe sepsis induced death. Mitochondria DNA (mtDNA) contain un-methylated cytosine phosphate guanine (CpG) motifs, which exhibit immune stimulatory capacities. The aim of this study was to investigate the role and mechanism of mtDNA release on lipopolysaccharide (LPS) induced acute lung injury (ALI) and systemic inflammation. Following LPS injection, plasma mtDNA copies peak at 8 h. Compared with wild-type (WT) mice, mtDNA in toll like receptor 4 knockout (TLR4 KO) mice were significantly decreased. MtDNA intra-peritoneal administration causes apparent ALI as demonstrated by increased lung injury score, bronchoalveolar lavage fluid (BALF) total protein and wet/dry (W/D) ratio; mtDNA injection also directly provokes systemic inflammation, as demonstrated by increased IL-1β, IL-6, high-mobility group protein B1 (HMGB1) level; while nuclear DNA (nDNA) could not induce apparent ALI and systemic inflammation. However, compared with WT mice, TLR4 KO could not protect from mtDNA induced ALI and systemic inflammation. Specific TLR9 inhibitor, ODN 2088 pretreatment can significantly attenuate mtDNA induced ALI and systemic inflammation, as demonstrated by improved lung injury score, decreased lung wet/dry ratio, BALF total protein concentration, and decreased systemic level of IL-1β, IL-6 and HMGB1. MtDNA administration activates the expression of p-P38 mitogen-activated protein kinases (MAPK) in lung tissue and specific TLR9 inhibitor pretreatment can attenuate this activation. Thus, LPS-induced mtDNA release occurs in a TLR4-dependent manner, and mtDNA causes acute lung injury and systemic inflammation in a TLR9-dependent and TLR4-independent manner. PMID:27589725

  18. DNA vaccines for aquacultured fish

    Lorenzen, Niels; LaPatra, S.E.

    2005-01-01

    fully addressed, although inherently the risks should not be any greater than with the commercial fish vaccines that are currently used. Present classification systems lack clarity in distinguishing DNA-vaccinated animals from genetically modified organisms (GMOs), which could raise issues in terms of...... licensing and public acceptance of the technology. The potential benefits of DNA vaccines for farmed fish include improved animal welfare, reduced environmental impacts of aquaculture activities, increased food quality and quantity, and more sustainable production. Testing under commercial production...

  19. Safety culture and public acceptance

    After the Chernobyl NPP accident a public acceptance has become a key factor in nuclear power development all over the world. Therefore, nuclear safety culture should be based not only on technical principles, responsibilities, supervision, regulatory provisions, emergency preparedness, but the public awareness of minimum risk during the operation and decommissioning of NPPs, radioactive waste management, etc. (author)

  20. Nitrogen trailer acceptance test report

    This Acceptance Test Report documents compliance with the requirements of specification WHC-S-0249. The equipment was tested according to WHC-SD-WM-ATP-108 Rev.0. The equipment being tested is a portable contained nitrogen supply. The test was conducted at Norco's facility

  1. Acceptance tests and commissioning measurements

    Following the installation of a therapy machine, be it an orthovoltage X ray unit, cobalt unit, linac or brachytherapy machine, in a radiotherapy clinic, the medical physicist must perform a series of measurements and tasks prior to placing the unit into clinical operation. These duties include acceptance testing and commissioning. Various dosimetric and radiation measuring instruments and techniques are discussed

  2. AAL- technology acceptance through experience

    Huldtgren, A.; Ascencio San Pedro, G.; Pohlmeyer, A.E.; Romero Herrera, N.A.

    2014-01-01

    Despite substantial research and development of Ambient Assisted Living (AAL) technologies, their acceptance remains low. This is partially caused by a lack of accounting for users' needs and values, and the social contexts these systems are to be embedded in. Participatory design has some potential

  3. Nitrogen trailer acceptance test report

    Kostelnik, A.J.

    1996-02-12

    This Acceptance Test Report documents compliance with the requirements of specification WHC-S-0249. The equipment was tested according to WHC-SD-WM-ATP-108 Rev.0. The equipment being tested is a portable contained nitrogen supply. The test was conducted at Norco`s facility.

  4. Euthanasia Acceptance: An Attitudinal Inquiry.

    Klopfer, Fredrick J.; Price, William F.

    The study presented was conducted to examine potential relationships between attitudes regarding the dying process, including acceptance of euthanasia, and other attitudinal or demographic attributes. The data of the survey was comprised of responses given by 331 respondents to a door-to-door interview. Results are discussed in terms of preferred…

  5. Worldwide nuclear revival and acceptance

    The paper outlines the current status and trends of the nuclear revival in Europe and abroad, the evolution of the public opinion in the last decade, and the interaction between the former and the latter. It emphasises the absolute priority of a professional communication and exchange to gain public acceptance. (orig.)

  6. DNA-PK assay

    Anderson, Carl W.; Connelly, Margery A.

    2004-10-12

    The present invention provides a method for detecting DNA-activated protein kinase (DNA-PK) activity in a biological sample. The method includes contacting a biological sample with a detectably-labeled phosphate donor and a synthetic peptide substrate defined by the following features to provide specific recognition and phosphorylation by DNA-PK: (1) a phosphate-accepting amino acid pair which may include serine-glutamine (Ser-Gln) (SQ), threonine-glutamine (Thr-Gln) (TQ), glutamine-serine (Gln-Ser) (QS), or glutamine-threonine (Gln-Thr) (QT); (2) enhancer amino acids which may include glutamic acid or glutamine immediately adjacent at the amino- or carboxyl- side of the amino acid pair and forming an amino acid pair-enhancer unit; (3) a first spacer sequence at the amino terminus of the amino acid pair-enhancer unit; (4) a second spacer sequence at the carboxyl terminus of the amino acid pair-enhancer unit, which spacer sequences may include any combination of amino acids that does not provide a phosphorylation site consensus sequence motif; and, (5) a tag moiety, which may be an amino acid sequence or another chemical entity that permits separating the synthetic peptide from the phosphate donor. A compostion and a kit for the detection of DNA-PK activity are also provided. Methods for detecting DNA, protein phosphatases and substances that alter the activity of DNA-PK are also provided. The present invention also provides a method of monitoring protein kinase and DNA-PK activity in living cells. -A composition and a kit for monitoring protein kinase activity in vitro and a composition and a kit for monitoring DNA-PK activities in living cells are also provided. A method for identifying agents that alter protein kinase activity in vitro and a method for identifying agents that alter DNA-PK activity in living cells are also provided.

  7. DNA vaccines

    Coban, Cevayir; Kobiyama, Kouji; Jounai, Nao; Tozuka, Miyuki; Ishii, Ken J.

    2013-01-01

    Since the introduction of DNA vaccines two decades ago, this attractive strategy has been hampered by its low immunogenicity in humans. Studies conducted to improve the immunogenicity of DNA vaccines have shown that understanding the mechanism of action of DNA vaccines might be the key to successfully improving their immunogenicity. Our current understanding is that DNA vaccines induce innate and adaptive immune responses in two ways: (1) encoded protein (or polypeptide) antigen(s) by the DNA...

  8. Consumer acceptance of irradiated food

    There was a widely held opinion during the 1970's and 1980's that consumers would be reluctant to purchase irradiated food, as it was perceived that consumers would confuse irradiated food with food contaminated by radionuclides. Indeed, a number of consumer attitude surveys conducted in several western countries during these two decades demonstrated that the concerns of consumers on irradiated food varied from very concerned to seriously concerned.This paper attempts to review parameters conducting in measuring consumer acceptance of irradiated food during the past three decades and to project the trends on this subject. It is believed that important lessons learned from past studies will guide further efforts to market irradiated food with wide consumer acceptance in the future. (Author)

  9. Consumer acceptance of irradiated food

    Loaharanu, P. [Head, Food Preservation Section, Joint FAO/ IAEA Division of Nuclear Techniques in Food and Agriculture, Wagramerstr. 5, A-1400, Vienna (Austria)

    1997-12-31

    There was a widely held opinion during the 1970`s and 1980`s that consumers would be reluctant to purchase irradiated food, as it was perceived that consumers would confuse irradiated food with food contaminated by radionuclides. Indeed, a number of consumer attitude surveys conducted in several western countries during these two decades demonstrated that the concerns of consumers on irradiated food varied from very concerned to seriously concerned.This paper attempts to review parameters conducting in measuring consumer acceptance of irradiated food during the past three decades and to project the trends on this subject. It is believed that important lessons learned from past studies will guide further efforts to market irradiated food with wide consumer acceptance in the future. (Author)

  10. Acceptability of reactors in space

    Buden, D.

    1981-04-01

    Reactors are the key to our future expansion into space. However, there has been some confusion in the public as to whether they are a safe and acceptable technology for use in space. The answer to these questions is explored. The US position is that when reactors are the preferred technical choice, that they can be used safely. In fact, it dies not appear that reactors add measurably to the risk associated with the Space Transportation System.

  11. Vaccine acceptance: The UK perspective

    Ford, John A; Mahgoub, Hamid; Shankar, Ananda Giri

    2013-01-01

    The United Kingdom has had a long history with vaccine acceptability dating back to Edward Jenner’s theory of small pox vaccination. More recently, the discredited, Wakefield study published in 1998 continues to cause MMR skepticism. In pregnant women pertussis vaccination has been considerably more successful than influenza vaccination. Influenza vaccine uptake in healthcare workers remains poor. The media, politicians, and health reforms have contributed to the mixed coverage for these vacc...

  12. Acceptability of reactors in space

    Reactors are the key to our future expansion into space. However, there has been some confusion in the public as to whether they are a safe and acceptable technology for use in space. The answer to these questions is explored. The US position is that when reactors are the preferred technical choice, that they can be used safely. In fact, it dies not appear that reactors add measurably to the risk associated with the Space Transportation System

  13. Acceptability of reactors in space

    Reactors are the key to our future expansion into space. However, there has been some confusion in the public as to whether they are a safe and acceptable technology for use in space. The answer to these questions is explored. The US position is that when reactors are the preferred technical choice, that they can be used safely. In fact, it does not appear that reactors add measurably to the risk associated with the Space Transportation System

  14. Parvovirus b19 DNA CpG dinucleotide methylation and epigenetic regulation of viral expression.

    Francesca Bonvicini

    Full Text Available CpG DNA methylation is one of the main epigenetic modifications playing a role in the control of gene expression. For DNA viruses whose genome has the ability to integrate in the host genome or to maintain as a latent episome, a correlation has been found between the extent of DNA methylation and viral quiescence. No information is available for Parvovirus B19, a human pathogenic virus, which is capable of both lytic and persistent infections. Within Parvovirus B19 genome, the inverted terminal regions display all the characteristic signatures of a genomic CpG island; therefore we hypothesised a role of CpG dinucleotide methylation in the regulation of viral genome expression.The analysis of CpG dinucleotide methylation of Parvovirus B19 DNA was carried out by an aptly designed quantitative real-time PCR assay on bisulfite-modified DNA. The effects of CpG methylation on the regulation of viral genome expression were first investigated by transfection of either unmethylated or in vitro methylated viral DNA in a model cell line, showing that methylation of viral DNA was correlated to lower expression levels of the viral genome. Then, in the course of in vitro infections in different cellular environments, it was observed that absence of viral expression and genome replication were both correlated to increasing levels of CpG methylation of viral DNA. Finally, the presence of CpG methylation was documented in viral DNA present in bioptic samples, indicating the occurrence and a possible role of this epigenetic modification in the course of natural infections.The presence of an epigenetic level of regulation of viral genome expression, possibly correlated to the silencing of the viral genome and contributing to the maintenance of the virus in tissues, can be relevant to the balance and outcome of the different types of infection associated to Parvovirus B19.

  15. DNA methylation analysis using CpG microarrays is impaired in benzopyrene exposed cells

    Epigenetic alterations have emerged as a key mechanism involved in tumorigenesis. These disruptions are partly due to environmental factors that change normal DNA methylation patterns necessary for transcriptional regulation and chromatin compaction. Microarray technologies are allowing environmentally susceptible epigenetic patterns to be mapped and the precise targets of environmentally induced alterations to be identified. Previously, we observed BaP-induced epigenetic events and cell cycle disruptions in breast cancer cell lines that included time- and concentration-dependent loss of proliferation as well as sequence-specific hypo- and hypermethylation events. In this present report, we further characterized epigenetic changes in BaP-exposed MCF-7 cells. We analyzed DNA methylation on a CpG island microarray platform with over 5400 unique genomic regions. Depleted and enriched microarray targets, representative of putative DNA methylation changes, were identified across the genome; however, subsequent sodium bisulfite analyses revealed no changes in DNA methylation at a number of these loci. Instead, we found that the identification of DNA methylation changes using this restriction enzyme-based microarray approach corresponded with the regions of DNA bound by the BaP derived DNA adducts. This DNA adduct formation occurs at both methylated and unmethylated CpG dinucleotides and affects PCR amplification during sample preparation. Our data suggest that caution should be exercised when interpreting data from comparative microarray experiments that rely on enzymatic reactions. These results are relevant to genome screening approaches involving environmental exposures in which DNA adduct formation at specific nucleotide sites may bias target acquisition and compromise the correct identification of epigenetically responsive genes

  16. DNA Methylation

    Alokail, Majed S.; Alenad, Amal M

    2015-01-01

    The DNA of E. coli contains 19,120 6-methyladenines and 12,045 5-methylcytosines in addition to the four regular bases and these are formed by the postreplicative action of three DNA methyltransferases. The majority of the methylated bases are formed by the Dam and Dcm methyltransferases encoded by the dam (DNA adenine methyltransferase) and dcm (DNA cytosine methyltransferase) genes. Although not essential, Dam methylation is important for strand discrimination during repair of replication e...

  17. DNA looping.

    Matthews, K S

    1992-01-01

    DNA-looping mechanisms are part of networks that regulate all aspects of DNA metabolism, including transcription, replication, and recombination. DNA looping is involved in regulation of transcriptional initiation in prokaryotic operons, including ara, gal, lac, and deo, and in phage systems. Similarly, in eukaryotic organisms, the effects of enhancers appear to be mediated at least in part by loop formation, and examples of DNA looping by hormone receptor proteins and developmental regulator...

  18. HP1 Is Involved in Regulating the Global Impact of DNA Methylation on Alternative Splicing

    Ahuvi Yearim

    2015-02-01

    Full Text Available The global impact of DNA methylation on alternative splicing is largely unknown. Using a genome-wide approach in wild-type and methylation-deficient embryonic stem cells, we found that DNA methylation can either enhance or silence exon recognition and affects the splicing of more than 20% of alternative exons. These exons are characterized by distinct genetic and epigenetic signatures. Alternative splicing regulation of a subset of these exons can be explained by heterochromatin protein 1 (HP1, which silences or enhances exon recognition in a position-dependent manner. We constructed an experimental system using site-specific targeting of a methylated/unmethylated gene and demonstrate a direct causal relationship between DNA methylation and alternative splicing. HP1 regulates this gene’s alternative splicing in a methylation-dependent manner by recruiting splicing factors to its methylated form. Our results demonstrate DNA methylation’s significant global influence on mRNA splicing and identify a specific mechanism of splicing regulation mediated by HP1.

  19. DNA structure

    Bowater, R

    2003-01-01

    Deoxyribonucleic acid (DNA) is a polymer of nucleotides. In the cell, DNA usually adopts a double-stranded helical form, with complementary base-pairing holding the two strands together. The most stable conformation is called B-form DNA, although other structures can occur under specific conditions.

  20. Fast Acceptance by Common Experience

    Nathan Berg

    2010-08-01

    Full Text Available Schelling (1969, 1971a,b, 1978 observed that macro-level patterns do not necessarily reflect micro-level intentions, desires or goals. In his classic model on neighborhood segregation which initiated a large and influential literature, individuals with no desire to be segregated from those who belong to other social groups nevertheless wind up clustering with their own type. Most extensions of Schelling's model have replicated this result. There is an important mismatch, however, between theory and observation, which has received relatively little attention. Whereas Schelling-inspired models typically predict large degrees of segregation starting from virtually any initial condition, the empirical literature documents considerable heterogeneity in measured levels of segregation. This paper introduces a mechanism that can produce significantly higher levels of integration and, therefore, brings predicted distributions of segregation more in line with real-world observation. As in the classic Schelling model, agents in a simulated world want to stay or move to a new location depending on the proportion of neighbors they judge to be acceptable. In contrast to the classic model, agents' classifications of their neighbors as acceptable or not depend lexicographically on recognition first and group type (e.g., ethnic stereotyping second. The FACE-recognition model nests classic Schelling: When agents have no recognition memory, judgments about the acceptability of a prospective neighbor rely solely on his or her group type (as in the Schelling model. A very small amount of recognition memory, however, eventually leads to different classifications that, in turn, produce dramatic macro-level effects resulting in significantly higher levels of integration. A novel implication of the FACE-recognition model concerns the large potential impact of policy interventions that generate modest numbers of face-to-face encounters with members of other social groups.

  1. Role of DNA profiling in forensic odontology.

    Sakari, S Leena; Jimson, Sudha; Masthan, K M K; Jacobina, Jenita

    2015-04-01

    The recent advances in DNA profiling have made DNA evidence to be more widely accepted in courts. This has revolutionized the aspect of forensic odontology. DNA profiling/DNA fingerprinting has come a long way from the conventional fingerprints. DNA that is responsible for all the cell's activities, yields valuable information both in the healthy and diseased individuals. When other means of traditional identification become impossible following mass calamities or fire explosions, teeth provide a rich source of DNA as they have a high chemical as well as physical resistance. The recent evolution in the isolation of DNA and the ways of running a DNA fingerprint are highlighted in this literature review. PMID:26015692

  2. Role of DNA profiling in forensic odontology

    S Leena Sakari

    2015-01-01

    Full Text Available The recent advances in DNA profiling have made DNA evidence to be more widely accepted in courts. This has revolutionized the aspect of forensic odontology. DNA profiling/DNA fingerprinting has come a long way from the conventional fingerprints. DNA that is responsible for all the cell′s activities, yields valuable information both in the healthy and diseased individuals. When other means of traditional identification become impossible following mass calamities or fire explosions, teeth provide a rich source of DNA as they have a high chemical as well as physical resistance. The recent evolution in the isolation of DNA and the ways of running a DNA fingerprint are highlighted in this literature review.

  3. Role of DNA profiling in forensic odontology

    Sakari, S. Leena; Jimson, Sudha; Masthan, K. M. K.; Jacobina, Jenita

    2015-01-01

    The recent advances in DNA profiling have made DNA evidence to be more widely accepted in courts. This has revolutionized the aspect of forensic odontology. DNA profiling/DNA fingerprinting has come a long way from the conventional fingerprints. DNA that is responsible for all the cell's activities, yields valuable information both in the healthy and diseased individuals. When other means of traditional identification become impossible following mass calamities or fire explosions, teeth provide a rich source of DNA as they have a high chemical as well as physical resistance. The recent evolution in the isolation of DNA and the ways of running a DNA fingerprint are highlighted in this literature review. PMID:26015692

  4. Wind energy and social acceptability

    This document was prepared as part of a decentralized collaboration between Quebec and France to share knowledge regarding strategies and best practices in wind power development. It reviewed the social acceptance of Quebec's wind power industry, particularly at the municipal level. The wind industry is growing rapidly in Quebec, and this growth has generated many reactions ranging from positive to negative. The purpose of this joint effort was to describe decision making steps to developing a wind turbine array. The history of wind development in Quebec was discussed along with the various hardware components required in a wind turbine and different types of installations. The key element in implementing wind turbine arrays is to establish public acceptance of the project, followed by a good regulatory framework to define the roles and responsibilities of participants. The production of electricity from wind turbines constitutes a clean and renewable source of energy. Although it is associated with a reduction in greenhouse gas emissions, this form of energy can also have negative environmental impacts, including noise. The revenues generated by wind parks are important factors in the decision making process. Two case studies in Quebec were presented. refs., tabs., figs.

  5. Policy formulation of public acceptance

    Since 1970, the new policy formulation for public acceptance of the new consideration on the location of electric power generation has been set and applied. The planning and the enforcement being conducted by local public organizations for the local economic build-up with plant location and also the adjustement of the requirements for fishery are two main specific characters in this new policy. The background of this new public acceptance policy, the history and the actual problems about the compensation for the location of power generation plants are reviewed. One new proposal, being recommended by the Policy and Science Laboratory to MITI in 1977 is explained. This is based on the method of promoting the location of power generation plants by public participation placing the redevelopment of regional societies as its basis. The problems concerning the industrial structures in farm villages, fishing villages and the areas of commerce and industry should be systematized, and explained from the viewpoint of outside impact, the characteristics of local areas and the location problems in this new proposal. Finally, the location process and its effectiveness should be put in order. (Nakai, Y.)

  6. Heat exchanger staybolt acceptance criteria

    The structural integrity demonstration of the primary coolant piping system includes evaluating the structural capacity of each component against a large break or equivalent Double-Ended Guillotine Break. A large break at the inlet or outlet heads of the heat exchangers would occur if the restraint members of the heads become inactive. The structural integrity of the heads is demonstrated by showing the redundant capacity of the staybolts to restrain the head at design conditions and under seismic loadings. The Savannah River Site heat exchanger head is attached to the tubesheet by 84 staybolts. Access to the staybolts is limited due to a welded seal cap over the staybolts. An ultrasonic testing (UT) inspection technique to provide an in-situ examination of the staybolts has recently been developed at SRS. Examination of the staybolts will be performed to ensure their service condition and configuration is within acceptance limits. An acceptance criteria methodology has been developed to disposition flaws reported in the staybolt inspections while ensuring adequate restraint capacity of the staybolts to maintain integrity of the heat exchanger heads against collapse. The methodology includes an approach for the baseline and periodic inspections of the staybolts. The heat exchanger head is analyzed with a three-dimensional finite element model. The restraint provided by the staybolts is evaluated for several postulated cases of inactive or missing staybolts. Evaluation of specific, inactive staybolt configurations based on the UT results can be performed with the finite element model and fracture methodology in this report

  7. DNA methylation profiles at precancerous stages associated with recurrence of lung adenocarcinoma.

    Takashi Sato

    Full Text Available The aim of this study was to clarify the significance of DNA methylation alterations at precancerous stages of lung adenocarcinoma. Using single-CpG resolution Infinium array, genome-wide DNA methylation analysis was performed in 36 samples of normal lung tissue obtained from patients without any primary lung tumor, 145 samples of non-cancerous lung tissue (N obtained from patients with lung adenocarcinomas, and 145 samples of tumorous tissue (T. Stepwise progression of DNA methylation alterations from normal lung tissue to non-cancerous lung tissue obtained from patients with lung adenocarcinomas, and then tumorous tissue samples, was observed at 3,270 CpG sites, suggesting that non-cancerous lung tissue obtained from patients with lung adenocarcinomas was at precancerous stages with DNA methylation alterations. At CpG sites of 2,083 genes, DNA methylation status in samples of non-cancerous lung tissue obtained from patients with lung adenocarcinomas was significantly correlated with recurrence after establishment of lung adenocarcinomas. Among such recurrence-related genes, 28 genes are normally unmethylated (average β-values based on Infinium assay in normal lung tissue samples was less than 0.2 and their DNA hypermethylation at precancerous stages was strengthened during progression to lung adenocarcinomas (Δβ(T-N>0.1. Among these 28 genes, we focused on 6 for which implications in transcription regulation, apoptosis or cell adhesion had been reported. DNA hypermethylation of the ADCY5, EVX1, GFRA1, PDE9A, and TBX20 genes resulted in reduced mRNA expression in tumorous tissue samples. 5-Aza-2'-deoxycytidine treatment of lung cancer cell lines restored the mRNA expression levels of these 5 genes. Reduced mRNA expression in tumorous tissue samples was significantly correlated with tumor aggressiveness. These data suggest that DNA methylation alterations at precancerous stages determine tumor aggressiveness and outcome through silencing of

  8. DNA Methylation Profiles at Precancerous Stages Associated with Recurrence of Lung Adenocarcinoma

    Sato, Takashi; Arai, Eri; Kohno, Takashi; Tsuta, Koji; Watanabe, Shun-ichi; Soejima, Kenzo; Betsuyaku, Tomoko; Kanai, Yae

    2013-01-01

    The aim of this study was to clarify the significance of DNA methylation alterations at precancerous stages of lung adenocarcinoma. Using single-CpG resolution Infinium array, genome-wide DNA methylation analysis was performed in 36 samples of normal lung tissue obtained from patients without any primary lung tumor, 145 samples of non-cancerous lung tissue (N) obtained from patients with lung adenocarcinomas, and 145 samples of tumorous tissue (T). Stepwise progression of DNA methylation alterations from normal lung tissue to non-cancerous lung tissue obtained from patients with lung adenocarcinomas, and then tumorous tissue samples, was observed at 3,270 CpG sites, suggesting that non-cancerous lung tissue obtained from patients with lung adenocarcinomas was at precancerous stages with DNA methylation alterations. At CpG sites of 2,083 genes, DNA methylation status in samples of non-cancerous lung tissue obtained from patients with lung adenocarcinomas was significantly correlated with recurrence after establishment of lung adenocarcinomas. Among such recurrence-related genes, 28 genes are normally unmethylated (average β-values based on Infinium assay in normal lung tissue samples was less than 0.2) and their DNA hypermethylation at precancerous stages was strengthened during progression to lung adenocarcinomas (ΔβT–N>0.1). Among these 28 genes, we focused on 6 for which implications in transcription regulation, apoptosis or cell adhesion had been reported. DNA hypermethylation of the ADCY5, EVX1, GFRA1, PDE9A, and TBX20 genes resulted in reduced mRNA expression in tumorous tissue samples. 5-Aza-2′-deoxycytidine treatment of lung cancer cell lines restored the mRNA expression levels of these 5 genes. Reduced mRNA expression in tumorous tissue samples was significantly correlated with tumor aggressiveness. These data suggest that DNA methylation alterations at precancerous stages determine tumor aggressiveness and outcome through silencing of specific genes

  9. Acceptance of Others, Feeling of Being Accepted and Striving for Being Accepted Among the Representatives of Different Kinds of Occupations

    Gergana Stanoeva

    2012-05-01

    Full Text Available This paper deals with an important issue related to the human attitudes and needs in interpersonal and professional aspects. The theoretical part deals with several psychological components of the self-esteem and esteem of the others – acceptance of the others, feeling of being accepted, need for approval. Some gender differences in manifestations of acceptance and feeling of being accepted at the workplace are discussed. This article presents some empirical data for the degree of acceptance of others, feeling of being accepted and the strive for being accepted among the representatives of helping, pedagogical, administrative and economic occupations, as well as non-qualified workers. The goals of the study were to reveal the interdependency between these constructs and to be found some significant differences between the representatives of the four groups of occupations. The methods of the first study were W. Fey’s scales “Acceptance of others”, and “How do I feel accepted by others”. The method of the second study was Crown and Marlowe Scale for Social Desirability. The results indicated some significant differences in acceptance of others and feeling of being accepted between the non-qualified workers and the representatives of helping, administrative and economic occupations. There were not any significant difference in strive for being accepted between the fouroccupational groups.

  10. PAGs - Public perception and acceptance

    Full text: While Protective Action Guides or PAGs have been a part of the lexicon of the radiation protection field for several decades, the concept of accepting higher levels of risk under certain situations has not received adequate scrutiny by the general public, the media or elected officials. Consequently there is a question as to how implementation of PAGs would be perceived by the above groups in the event that such implementation became necessary. A personal case in point involves the response of an executive in the food industry. When the concept of selling a food product meeting the PAGs was explained his response was, 'we won't sell a contaminated product, we would dump the unprocessed raw food. Our industry image is that of a natural unadulterated food'. While this may be an isolated view, there is a need to determine what is the perception and consequently what would be the response if PAGs were implemented today. If the response was negative by anyone of the three groups listed previously, then there is an obvious need for a program to assure receptiveness by those concerned. However, this may face formidable obstacles. This is because the terms radiation and radioactive have gained generally negative word associations, e.g. 'deadly' radiation and radioactive 'desert'. The former term was recently heard in a taped presentation at a Museum of Natural History on a completely unrelated subject. The latter term was part of a recent article heading in the Wall Street Journal. Incidentally the article was discussing television. Thus beyond the scientific issues of setting PAGs and the administrative and procedural issues of implementing PAGs there is the issue of society's understanding and acceptance of PAGs. Particularly, how can such understanding and acceptance be achieved in a situation which is associated with an actual or perceived radiation emergency? These are not questions that radiation or agricultural scientists can answer alone. These are

  11. Studying Student Teachers' Acceptance of Role Responsibility.

    Davis, Michael D.; Davis, Concetta M.

    1980-01-01

    There is variance in the way in which student teachers accept responsibility for the teaching act. This study explains why some variables may affect student teachers' acceptance of role responsibilities. (CM)

  12. Decision modeling and acceptance criteria

    Ditlevsen, Ove Dalager

    2003-01-01

    formulation of decision criteria and public acceptance criteria connected to risk analysis of technical operations that may endanger human life and property. Public restrictions on the decisions concerning the design, construction and managing of the technical operation have in the past been imposed on the...... expected gain rate. The public money equivalent of a human life is assessed by use of a recently in (Nathwani JS, Lind NC, Pandey MD. Afordable safety by choice: the life quality method. Waterloo, Ontario, Canada: Institute for Risk Research, University of Waterloo, 1997) suggested Life Quality Index (LQI......) that combines wealth in terms of Gross Domestic Product per person, life expectancy at birth, and yearly work time into a single number. The philosophy behind the published evaluations is that the prevention of a loss of a life is counteracted by a cost such that the LQI remains unchanged (Skjong R...

  13. Nanocarriers for DNA Vaccines: Co-Delivery of TLR-9 and NLR-2 Ligands Leads to Synergistic Enhancement of Proinflammatory Cytokine Release

    Johanna Poecheim

    2015-12-01

    Full Text Available Adjuvants enhance immunogenicity of vaccines through either targeted antigen delivery or stimulation of immune receptors. Three cationic nanoparticle formulations were evaluated for their potential as carriers for a DNA vaccine, and muramyl dipeptide (MDP as immunostimulatory agent, to induce and increase immunogenicity of Mycobacterium tuberculosis antigen encoding plasmid DNA (pDNA. The formulations included (1 trimethyl chitosan (TMC nanoparticles, (2 a squalene-in-water nanoemulsion, and (3 a mineral oil-in-water nanoemulsion. The adjuvant effect of the pDNA-nanocomplexes was evaluated by serum antibody analysis in immunized mice. All three carriers display a strong adjuvant effect, however, only TMC nanoparticles were capable to bias immune responses towards Th1. pDNA naturally contains immunostimulatory unmethylated CpG motifs that are recognized by Toll-like receptor 9 (TLR-9. In mechanistic in vitro studies, activation of TLR-9 and the ability to enhance immunogenicity by simultaneously targeting TLR-9 and NOD-like receptor 2 (NLR-2 was determined by proinflammatory cytokine release in RAW264.7 macrophages. pDNA in combination with MDP was shown to significantly increase proinflammatory cytokine release in a synergistic manner, dependent on NLR-2 activation. In summary, novel pDNA-Ag85A loaded nanoparticle formulations, which induce antigen specific immune responses in mice were developed, taking advantage of the synergistic combinations of TLR and NLR agonists to increase the adjuvanticity of the carriers used.

  14. Nuclear wastes and public acceptance

    A new approach to the storage of nuclear wastes is described. Certain criteria for a nuclear waste storage system that is based on ideas of technical soundess and public acceptability are set forth. These criteria are 1.) the wastes must be reliably contained at all times, 2.) the containers must be retrievable and maintainable, 3.) the storage facility must also provide isolation from external events and must also permit careful control of human access, 4.) the storage facility and containers must have plausible or demonstratble likelihood of lasting for 100 years, and 5.) the storage system should be able to accept and retrieve both processed waste and spent fuel elements interchangeably. A specific storage system concept that is based on proved data and that meets the 5 criteria is described. The waste, either glassified high-level waste or spent fuel-fuel bundles from which the end structures have been removed, is stored in sealed stainless steel containers, which is sealed in a second sealed container made of a durable metal such as Ti. The space between the two containers is filled with a gas that can be detected at very low concentrations. These containers are stored in a tunnel excavated into the side of a convenient mountain. The tunnel is excavated above flood level, is accessible by rail and/or road, and is designed for self-draining. A free-standing inner lining is constructed within the tunnel. Offset vertical shafts provide for ventilation. Continuous monitoring leak detectors are maintained in the tunnel and in the stack

  15. DNA methylation status of H19 and Xist genos in lungs of somatic cell nuclear transfer bovines

    CHEN Jie; LI DongJie; LIU YanQin; ZHANG Cui; DAI YunPing; LI ShiJie; LINing

    2008-01-01

    In somatic cell nuclear transfer (SCNT) technologies, the donor cell's nuclei need to be epigenetically reprogrammed for embryonic development. The incomplete reprogramming of donor cell nuclei has been implicated as a primary reason for the low efficiency of SCNT. DNA methylation is a major epige- netic modification of the genome that regulates crucial aspects of genome function, including estab-lishment of genomic imprinting. In order to make sure whether the DNA methylation reprogramming is efficient in SCNT animals, we analyzed the DNA methylation status of two imprinting genes, H19 and Xist, in lungs of deceased SCNT bovines that died within 48 h of birth using bisulfite sequencing analysis. Our findings demonstrated that cloned bovines showed significantly lower DNA methylation of H19 than controls (P<0.05), and three tested CpGs sites (1, 2, 3) exhibited unmethylation in one cloned bovine (9C3); however, Xist showed similar DNA methylation levels between clones and con- trols, and both showed hypermethylation (96.11% and 86.67%).

  16. The safety and social acceptance of novel foods.

    Moseley, B E

    1999-09-15

    The regulatory processes employed in the UK and the European Union to assess the safety of novel foods and novel food ingredients, including those resulting from the application of recombinant DNA technology (genetically modified foods), are described. Examples are given of yeasts that have been genetically modified and can be used in food and drink manufacture and food enzymes derived from genetically modified microorganisms that have been deemed safe for use by the UK regulatory system. Social acceptance of such novel foods or food ingredients is not uniform in countries of the developed world. Consumer concerns can be based on ethical considerations (scientists "playing God") or safety worries ("more testing needs to be done"). The general acceptance of such foods and food ingredients in Europe is still unclear. PMID:10488841

  17. Older Adults' Acceptance of Information Technology

    Wang, Lin; Rau, Pei-Luen Patrick; Salvendy, Gavriel

    2011-01-01

    This study investigated variables contributing to older adults' information technology acceptance through a survey, which was used to find factors explaining and predicting older adults' information technology acceptance behaviors. Four factors, including needs satisfaction, perceived usability, support availability, and public acceptance, were…

  18. DNA Immunization

    Wang, Shixia; Lu, Shan

    2013-01-01

    DNA immunization was discovered in early 1990s and its use has been expanded from vaccine studies to a broader range of biomedical research, such as the generation of high quality polyclonal and monoclonal antibodies as research reagents. In this unit, three common DNA immunization methods are described: needle injection, electroporation and gene gun. In addition, several common considerations related to DNA immunization are discussed.

  19. DNA deoxyribophosphodiesterase.

    Franklin, W A; Lindahl, T

    1988-01-01

    A previously unrecognized enzyme acting on damaged termini in DNA is present in Escherichia coli. The enzyme catalyses the hydrolytic release of 2-deoxyribose-5-phosphate from single-strand interruptions in DNA with a base-free residue on the 5' side. The partly purified protein appears to be free from endonuclease activity for apurinic/apyrimidinic sites, exonuclease activity and DNA 5'-phosphatase activity. The enzyme has a mol. wt of approximately 50,000-55,000 and has been termed DNA deox...

  20. Nuclear waste in public acceptance

    The existing problem on a faithful acceptance of nuclear information by population is connected, to a considerable extent, with a bad nuclear 'reputation' because of a great amount of misrepresented and false information from 'the greens'. In contrast to a bare style of professionals often neglecting an emotional perception, a loud voice of 'the greens' appeals both to the head, and to the heart of the audience. People pattern their behaviour weakly on problems of safe application of different irradiation sources in industry, conditions of life, medicine and everyday life. Radiation danger of some sources is often exaggerated (computers, nuclear technologies, radiation treatment) and the danger of the others is, on the contrary, underestimated (nuclear and roentgen methods of diagnostics and medical treatment). The majority of our citizens do not know which level of radiation is normal and safe, which ways radioactive substances intake into the organism of a human being and how to diminish the dose load on the organism by simple measures. Only specialists can be orientated themselves in a great number of radiation units. Low level of knowledge of the population and false conceptions are connected with the fact that they are mainly informed about nuclear technologies from mass media, where the voice of 'Greenpeace' is loudly sounded, but they often give misrepresented and false information doing it in the very emotional form. In contrast to them, scientists-professionals often ignore a sensitive part of apprehending of information and do not attach importance to it. As a rule, the style of specialists is of a serious academician character when they meet with the public. People preconception to nuclear waste and distrust to a positive information concerning nuclear technologies are explained, to a considerable extent, by a bivalent type of thinking when people operate by two opposite conceptions only, such as 'there is' or 'there is not' (there is or there is not

  1. Human Insulin from Recombinant DNA Technology

    Johnson, Irving S.

    1983-02-01

    Human insulin produced by recombinant DNA technology is the first commercial health care product derived from this technology. Work on this product was initiated before there were federal guidelines for large-scale recombinant DNA work or commercial development of recombinant DNA products. The steps taken to facilitate acceptance of large-scale work and proof of the identity and safety of such a product are described. While basic studies in recombinant DNA technology will continue to have a profound impact on research in the life sciences, commercial applications may well be controlled by economic conditions and the availability of investment capital.

  2. Acceptability of GM foods among Pakistani consumers.

    Ali, Akhter; Rahut, Dil Bahadur; Imtiaz, Muhammad

    2016-04-01

    In Pakistan majority of the consumers do not have information about genetically modified (GM) foods. In developing countries particularly in Pakistan few studies have focused on consumers' acceptability about GM foods. Using comprehensive primary dataset collected from 320 consumers in 2013 from Pakistan, this study analyzes the determinants of consumers' acceptability of GM foods. The data was analyzed by employing the bivariate probit model and censored least absolute deviation (CLAD) models. The empirical results indicated that urban consumers are more aware of GM foods compared to rural consumers. The acceptance of GM foods was more among females' consumers as compared to male consumers. In addition, the older consumers were more willing to accept GM food compared to young consumers. The acceptability of GM foods was also higher among wealthier households. Low price is the key factor leading to the acceptability of GM foods. The acceptability of the GM foods also reduces the risks among Pakistani consumers. PMID:27494790

  3. Consumer acceptance of irradiated foods

    Although the first experiments on food irradiation were carried out in 1916 in Sweden, food irradiation, is for consumers, a relatively new technology. From the sixties food irradiation has been applied more and more, so that the consumer movement has become alert to this technology. Since then a lot of controversies have arisen in the literature about wholesomeness, safety, effects, etc. Food irradiation is currently permitted on a small scale in about 30 countries; in some countries or states food irradiation has been put under a ban (e.g. Australia, New Zealand, New Jersey). The World Health Organization (WHO) and the Food and Agriculture Organization of the United Nations (FAO) have, however, chosen food irradiation as a safe and sound method for preserving and improving the safety of food. Reactions on the part of the consumer organizations of many countries are however not in favour of or are even opposed to food irradiation. In this chapter consumer acceptance related to technological developments is described, then the convergence of the consumer movement on public opinion and concern on food irradiation is discussed. The need for labelling of irradiated food products is discussed and finally recommendations are given of ways to change consumers attitudes to food irradiation. (author)

  4. Toxic chemical risk acceptance criteria

    This paper presents recommendations of a subcommittee of the Westinghouse M ampersand 0 Nuclear Facility Safety Committee concerning toxic chemical risk acceptance criteria. Two sets of criteria have been developed, one for use in the hazard classification of facilities, and the second for use in comparing risks in DOE non-reactor nuclear facility Safety Analysis Reports. The Emergency Response Planning Guideline (ERPG) values are intended to provide estimates of concentration ranges for specific chemicals above which exposure would be expected to lead to adverse heath effects of increasing severity for ERPG-1, -2, and -3s. The subcommittee recommends that criteria for hazard class or risk range be based on ERPGs for all chemicals. Probability-based Incremental Cancer Risk (ICR) criteria are recommended for additional analyses of risks from all known or suspected human carcinogens. Criteria are given for both on-site and off-site exposure. The subcommittee also recommends that the 5-minute peak concentration be compared with the relevant criterion with no adjustment for exposure time. Since ERPGs are available for only a limited number of chemicals, the subcommittee has developed a proposed hierarchy of concentration limit parameters for the different criteria

  5. Issues on accepting nuclear energy

    Nuclear power has been promoted so far as a national project to be an energy source sharing a large weight in the future and is also expected recently to be a means to suppress the global warming affected by the use of fossil fuels. From a stand point opposing to or cautious of the promotion of its extensive use, various issues on its incompatibility to the society such as technical problems pointed out that radioactivity miss-control may cause hazards, energy problems, political problems, cultural life problems, etc. are raised. Also in site areas, pros and cons on the evaluation of its contribution are spreading. However, the area of the issues is wide-spread and sometime too difficult to understand because of its specialty or barriers such as conviction and fixed distrust and so it is often seen that the controversies are lead to be governed by irritation or abandonment that ones argument is not understood by the party. In the social situation in which common interests for various stand points are hard to find, it looks only way for finding the direction of any decision in a political issue to mutually know the arguments through discussion as much as possible, correct erroneous understandings and expand the area of agreement. Hear, various issues on accepting nuclear power from a variety of stand points and view angles are summarized so as to be referred by various engineers and non-engineers to let the uselessly continuing deadlock proceed toward meaningful agreement. (author)

  6. Cleaving DNA with DNA

    Carmi, Nir; Balkhi, Shameelah R.; Breaker, Ronald R.

    1998-01-01

    A DNA structure is described that can cleave single-stranded DNA oligonucleotides in the presence of ionic copper. This “deoxyribozyme” can self-cleave or can operate as a bimolecular complex that simultaneously makes use of duplex and triplex interactions to bind and cleave separate DNA substrates. Bimolecular deoxyribozyme-mediated strand scission proceeds with a kobs of 0.2 min−1, whereas the corresponding uncatalyzed reaction could not be detected. The duplex and triplex recognition domai...

  7. DNA glue

    Filichev, Vyacheslav V; Astakhova, Irina V.; Malakhov, Andrei D.;

    2008-01-01

    Significant alterations in thermal stability of parallel DNA triplexes and antiparallel duplexes were observed upon changing the attachment of ethynylpyrenes from para to ortho in the structure of phenylmethylglycerol inserted as a bulge into DNA (TINA). Insertions of two ortho-TINAs as a pseudo...

  8. DNA probes

    The creation of DNA probes for detection of specific nucleotide segments differs from ligand detection in that it is a chemical rather than an immunological reaction. Complementary DNA or RNA is used in place of the antibody and is labelled with 32P. So far, DNA probes have been successfully employed in the diagnosis of inherited disorders, infectious diseases, and for identification of human oncogenes. The latest approach to the diagnosis of communicable and parasitic infections is based on the use of deoxyribonucleic acid (DNA) probes. The genetic information of all cells is encoded by DNA and DNA probe approach to identification of pathogens is unique because the focus of the method is the nucleic acid content of the organism rather than the products that the nucleic acid encodes. Since every properly classified species has some unique nucleotide sequences that distinguish it from every other species, each organism's genetic composition is in essence a finger print that can be used for its identification. In addition to this specificity, DNA probes offer other advantages in that pathogens may be identified directly in clinical specimens

  9. [DNA computing].

    Błasiak, Janusz; Krasiński, Tadeusz; Popławski, Tomasz; Sakowski, Sebastian

    2011-01-01

    Biocomputers can be an alternative for traditional "silicon-based" computers, which continuous development may be limited due to further miniaturization (imposed by the Heisenberg Uncertainty Principle) and increasing the amount of information between the central processing unit and the main memory (von Neuman bottleneck). The idea of DNA computing came true for the first time in 1994, when Adleman solved the Hamiltonian Path Problem using short DNA oligomers and DNA ligase. In the early 2000s a series of biocomputer models was presented with a seminal work of Shapiro and his colleguas who presented molecular 2 state finite automaton, in which the restriction enzyme, FokI, constituted hardware and short DNA oligomers were software as well as input/output signals. DNA molecules provided also energy for this machine. DNA computing can be exploited in many applications, from study on the gene expression pattern to diagnosis and therapy of cancer. The idea of DNA computing is still in progress in research both in vitro and in vivo and at least promising results of these research allow to have a hope for a breakthrough in the computer science. PMID:21735816

  10. Acceptance and Commitment Therapy: Distinctive Features

    Flaxman, Paul E.; Blackledge, J. T.; Bond, Frank W.

    2010-01-01

    What are the distinctive theoretical and practical features of acceptance and commitment therapy? Acceptance and commitment therapy (ACT) is a modern behaviour therapy that uses acceptance and mindfulness interventions alongside commitment and behaviour change strategies to enhance psychological flexibility. Psychological flexibility refers to the ability to contact the present moment and change or persist in behaviour that serves one’s personally chosen values. Divided into two secti...

  11. Mindfulness, Acceptance and Catastrophizing in Chronic Pain

    de Boer, Maaike J.; Steinhagen, Hannemike E.; Versteegen, Gerbrig J.; Struys, Michel M.R.F.; Sanderman, Robbert

    2014-01-01

    Objectives: Catastrophizing is often the primary target of the cognitive-behavioral treatment of chronic pain. Recent literature on acceptance and commitment therapy (ACT) suggests an important role in the pain experience for the concepts mindfulness and acceptance. The aim of this study is to examine the influence of mindfulness and general psychological acceptance on pain-related catastrophizing in patients with chronic pain. Methods: A cross-sectional survey was conducted, including 87 chr...

  12. Personal values and intention to accept ICT

    Harfouche, Antoine; Kalika, Michel

    2008-01-01

    This work-in-progress develops an integral model of individuals’ intention to accept Information and Communication Technology innovations (ICTIA model). It captures the influence of different external and internal variables on ICT’s acceptance and use at the first stages of the acceptance process. The model focuses on the association between (1) ICT perceived attributes and perceived characteristics of using ICT, (2) individual’s personal variables, specifically personal values, (3) environme...

  13. The Relationship Between Treatment Acceptability and Familism

    Joy R. Pemberton; Joaquin Borrego, Jr.

    2005-01-01

    Many studies have examined the acceptability of treatments for children with disruptive behaviors. However, few studies to date have tested the effects of home environment variables such as family support on treatment acceptability. In the current study, parents’ level of familism was used to predict the ir willingness toaccept several behavioral child treatment techniques. Eighty-two parents completed measures related to familism, child behavior problems, parenting stress, and acceptance of ...

  14. The acceptance of risks in modern civilization

    A systematic analysis or even consistent theory of the acceptance of risks is missing. So far it is limited to narrow subjects like economy, insurance and technology. In connection with the acceptance of technical risks the pros and cons of technology-assessment are dealt with. The 'gentle' way of technology has risks too, which at least jeopardize our prosperity. The acceptance of every risk in the end presupposes a sober consideration of unavoidable dangers and the yielded profit. (DG)

  15. DNA methylation regulates expression of VEGF-R2 (KDR) and VEGF-R3 (FLT4)

    Vascular Endothelial Growth Factors (VEGFs) and their receptors (VEGF-Rs) are important regulators for angiogenesis and lymphangiogenesis. VEGFs and VEGF-Rs are not only expressed on endothelial cells but also on various subtypes of solid tumors and leukemias contributing to the growth of the malignant cells. This study was performed to examine whether VEGF-R2 (KDR) and VEGF-R3 (FLT4) are regulated by DNA methylation. Real-time (RT) PCR analysis was performed to quantify KDR and FLT4 expression in some ninety leukemia/lymphoma cell lines, human umbilical vein endothelial cells (HUVECs) and dermal microvascular endothelial cells (HDMECs). Western blot analyses and flow cytometric analyses confirmed results at the protein level. After bisulfite conversion of DNA we determined the methylation status of KDR and FLT4 by DNA sequencing and by methylation specific PCR (MSP). Western blot analyses were performed to examine the effect of VEGF-C on p42/44 MAPK activation. Expression of KDR and FLT4 was observed in cell lines from various leukemic entities, but not in lymphoma cell lines: 16% (10/62) of the leukemia cell lines expressed KDR, 42% (27/65) were FLT4 positive. None of thirty cell lines representing six lymphoma subtypes showed more than marginal expression of KDR or FLT4. Western blot analyses confirmed KDR and FLT4 protein expression in HDMECs, HUVECs and in cell lines with high VEGF-R mRNA levels. Mature VEGF-C induced p42/44 MAPK activation in the KDR- /FLT4+ cell line OCI-AML1 verifying the model character of this cell line for VEGF-C signal transduction studies. Bisulfite sequencing and MSP revealed that GpG islands in the promoter regions of KDR and FLT4 were unmethylated in HUVECs, HDMECs and KDR+ and FLT4+ cell lines, whereas methylated cell lines did not express these genes. In hypermethylated cell lines, KDR and FLT4 were re-inducible by treatment with the DNA demethylating agent 5-Aza-2'deoxycytidine, confirming epigenetic regulation of both genes

  16. Food allergy: Stakeholder perspectives on acceptable risk

    Madsen, Charlotte Bernhard; Crevel, René; Chan, Chun-Han;

    2010-01-01

    We have reached a point where it is difficult to improve food allergy risk management without an agreement on levels of acceptable risk. This paper presents and discusses the perspectives of the different stakeholders (allergic consumers, health professionals, public authorities and the food...... industry) on acceptable risk in food allergy. Understanding where these perspectives diverge and even conflict may help develop an approach to define what is acceptable. Uncertainty about food allergy, its consequences and how to manage them is the common denominator of the stakeholders’ views. In patients...... not a realistic possibility, consensus on acceptable risk will be needed....

  17. 2013 SYR Accepted Poster Abstracts.

    2013-01-01

    SYR 2013 Accepted Poster abstracts: 1. Benefits of Yoga as a Wellness Practice in a Veterans Affairs (VA) Health Care Setting: If You Build It, Will They Come? 2. Yoga-based Psychotherapy Group With Urban Youth Exposed to Trauma. 3. Embodied Health: The Effects of a Mind�Body Course for Medical Students. 4. Interoceptive Awareness and Vegetable Intake After a Yoga and Stress Management Intervention. 5. Yoga Reduces Performance Anxiety in Adolescent Musicians. 6. Designing and Implementing a Therapeutic Yoga Program for Older Women With Knee Osteoarthritis. 7. Yoga and Life Skills Eating Disorder Prevention Among 5th Grade Females: A Controlled Trial. 8. A Randomized, Controlled Trial Comparing the Impact of Yoga and Physical Education on the Emotional and Behavioral Functioning of Middle School Children. 9. Feasibility of a Multisite, Community based Randomized Study of Yoga and Wellness Education for Women With Breast Cancer Undergoing Chemotherapy. 10. A Delphi Study for the Development of Protocol Guidelines for Yoga Interventions in Mental Health. 11. Impact Investigation of Breathwalk Daily Practice: Canada�India Collaborative Study. 12. Yoga Improves Distress, Fatigue, and Insomnia in Older Veteran Cancer Survivors: Results of a Pilot Study. 13. Assessment of Kundalini Mantra and Meditation as an Adjunctive Treatment With Mental Health Consumers. 14. Kundalini Yoga Therapy Versus Cognitive Behavior Therapy for Generalized Anxiety Disorder and Co-Occurring Mood Disorder. 15. Baseline Differences in Women Versus Men Initiating Yoga Programs to Aid Smoking Cessation: Quitting in Balance Versus QuitStrong. 16. Pranayam Practice: Impact on Focus and Everyday Life of Work and Relationships. 17. Participation in a Tailored Yoga Program is Associated With Improved Physical Health in Persons With Arthritis. 18. Effects of Yoga on Blood Pressure: Systematic Review and Meta-analysis. 19. A Quasi-experimental Trial of a Yoga based Intervention to Reduce Stress and

  18. DNA methylation

    Williams, Kristine; Christensen, Jesper; Helin, Kristian

    2012-01-01

    DNA methylation is involved in key cellular processes, including X-chromosome inactivation, imprinting and transcriptional silencing of specific genes and repetitive elements. DNA methylation patterns are frequently perturbed in human diseases such as imprinting disorders and cancer. The recent...... discovery that the three members of the TET protein family can convert 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) has provided a potential mechanism leading to DNA demethylation. Moreover, the demonstration that TET2 is frequently mutated in haematopoietic tumours suggests that the TET...... proteins are important regulators of cellular identity. Here, we review the current knowledge regarding the function of the TET proteins, and discuss various mechanisms by which they contribute to transcriptional control. We propose that the TET proteins have an important role in regulating DNA methylation...

  19. DNA data

    National Oceanic and Atmospheric Administration, Department of Commerce — Raw DNA chromatogram data produced by the ABI 373, 377, 3130 and 3730 automated sequencing machines in ABI format. These are from fish (primarily Sebastes spp.,...

  20. Ancient DNA

    Willerslev, Eske; Cooper, Alan

    2004-01-01

    In the past two decades, ancient DNA research has progressed from the retrieval of small fragments of mitochondrial DNA from a few late Holocene specimens, to large-scale studies of ancient populations, phenotypically important nuclear loci, and even whole mitochondrial genome sequences of extinct species. However, the field is still regularly marred by erroneous reports, which underestimate the extent of contamination within laboratories and samples themselves. An improved understanding of t...

  1. DNA Photolyasen

    Maul, Melanie

    2009-01-01

    Neben der fehlerfreien Weitergabe der genetischen Information während der Zellteilung durch einen intakten Replikationsapparat, ist auch die Aufrechterhaltung der genetischen Integrität der DNA durch Reparaturenzyme entscheidend für das Überleben der Zellen, sowie für einen gesunden Organismus. Um die genomische Integrität zu wahren, entwickelten sich im Laufe der Evolution verschiedene Mechanismen, u.a. die Exzisionreparatur von geschädigter DNA oder die direkte chemische R...

  2. DNA damage

    Kumari, Sunita; Rastogi, Rajesh P.; Singh, Kanchan L.; Singh, Shailendra P; Sinha, Rajeshwar P.

    2008-01-01

    Even under the best of circumstances, DNA is constantly subjected to chemical modifications. Several types of DNA damage such as SSB (single strand break), DSB (double strand break), CPDs (cyclobutane pyrimidine dimers), 6-4PPs (6-4 photoproducts) and their Dewar valence isomers have been identified that result from alkylating agents, hydrolytic deamination, free radicals and reactive oxygen species formed by various photochemical processes including UV radiation. There are a n...

  3. DNA expressions - A formal notation for DNA

    Vliet, Rudy van

    2015-01-01

    We describe a formal notation for DNA molecules that may contain nicks and gaps. The resulting DNA expressions denote formal DNA molecules. Different DNA expressions may denote the same molecule. Such DNA expressions are called equivalent. We examine which DNA expressions are minimal, which

  4. Nevada Test Site Waste Acceptance Criteria (NTSWAC)

    This document establishes the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office, Nevada Test Site Waste Acceptance Criteria (NTSWAC). The NTSWAC provides the requirements, terms, and conditions under which the Nevada Test Site will accept low-level radioactive (LLW) and LLW Mixed Waste (MW) for disposal

  5. Mini-DACS acceptance test report

    The attached document is the acceptance test summary report, of the mini-DACS, that was used to test the spare mixer pump for tank 241-SY-101, at the maintenance and storage facility (MASF). It includes a copy of the acceptance test procedure (ATP) and all pertaining ECNS

  6. A Distributive Model of Treatment Acceptability

    Carter, Stacy L.

    2008-01-01

    A model of treatment acceptability is proposed that distributes overall treatment acceptability into three separate categories of influence. The categories are comprised of societal influences, consultant influences, and influences associated with consumers of treatments. Each of these categories are defined and their inter-relationships within…

  7. 34 CFR 668.57 - Acceptable documentation.

    2010-07-01

    ... 34 Education 3 2010-07-01 2010-07-01 false Acceptable documentation. 668.57 Section 668.57... Application Information § 668.57 Acceptable documentation. (a) Adjusted Gross Income (AGI), income earned from... applicant, the documentation set forth in paragraph (a)(4) of this section if the individual for the...

  8. Mindfulness, acceptance and catastrophizing in chronic pain.

    Maaike J de Boer

    Full Text Available OBJECTIVES: Catastrophizing is often the primary target of the cognitive-behavioral treatment of chronic pain. Recent literature on acceptance and commitment therapy (ACT suggests an important role in the pain experience for the concepts mindfulness and acceptance. The aim of this study is to examine the influence of mindfulness and general psychological acceptance on pain-related catastrophizing in patients with chronic pain. METHODS: A cross-sectional survey was conducted, including 87 chronic pain patients from an academic outpatient pain center. RESULTS: The results show that general psychological acceptance (measured with the AAQ-II is a strong predictor of pain-related catastrophizing, independent of gender, age and pain intensity. Mindfulness (measured with the MAAS did not predict levels of pain-related catastrophizing. DISCUSSION: Acceptance of psychological experiences outside of pain itself is related to catastrophizing. Thus, acceptance seems to play a role in the pain experience and should be part of the treatment of chronic pain. The focus of the ACT treatment of chronic pain does not necessarily have to be on acceptance of pain per se, but may be aimed at acceptance of unwanted experiences in general. Mindfulness in the sense of "acting with awareness" is however not related to catastrophizing. Based on our research findings in comparisons with those of other authors, we recommend a broader conceptualization of mindfulness and the use of a multifaceted questionnaire for mindfulness instead of the unidimensional MAAS.

  9. Enzyme Reactions and Acceptability of Plant Foods.

    Palmer, James K.

    1984-01-01

    Provides an overview of enzyme reactions which contribute to the character and acceptability of plant foods. A detailed discussion of polyphenoloxidase is also provided as an example of an enzyme which can markedly affect the character and acceptability of such foods. (JN)

  10. Acceptable Channel Switching Delays for Mobile TV

    Fleury, Alexandre; Pedersen, Jakob Schou; Larsen, Lars Bo

    2011-01-01

    This paper presents a user study investigating the acceptability of channel switching delays on mobile television systems. The authors first review the previous work in the area, then propose a study design and present results from its implementation, focusing on the overall acceptability threshold...

  11. 49 CFR 193.2303 - Construction acceptance.

    2010-10-01

    ...: FEDERAL SAFETY STANDARDS Construction § 193.2303 Construction acceptance. No person may place in service... 49 Transportation 3 2010-10-01 2010-10-01 false Construction acceptance. 193.2303 Section 193.2303 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS...

  12. Heavy Metal, Religiosity, and Suicide Acceptability.

    Stack, Steven

    1998-01-01

    Reports on data taken from the General Social Survey that found a link between "heavy metal" rock fanship and suicide acceptability. Finds that relationship becomes nonsignificant once level of religiosity is controlled. Heavy metal fans are low in religiosity, which contributes to greater suicide acceptability. (Author/JDM)

  13. Hanford Site liquid waste acceptance criteria

    This document provides the waste acceptance criteria for liquid waste managed by Waste Management Federal Services of Hanford, Inc. (WMH). These waste acceptance criteria address the various requirements to operate a facility in compliance with applicable environmental, safety, and operational requirements. This document also addresses the sitewide miscellaneous streams program

  14. Nevada Test Site Waste Acceptance Criteria (NTSWAC)

    NNSA/NSO Waste Management Project

    2008-06-01

    This document establishes the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office, Nevada Test Site Waste Acceptance Criteria (NTSWAC). The NTSWAC provides the requirements, terms, and conditions under which the Nevada Test Site will accept low-level radioactive (LLW) and LLW Mixed Waste (MW) for disposal.

  15. Differences in HIV vaccine acceptability between genders.

    Kakinami, Lisa; Newman, Peter A; Lee, Sung-Jae; Duan, Naihua

    2008-05-01

    The development of safe and efficacious preventive HIV vaccines offers the best long-term hope of controlling the AIDS pandemic. Nevertheless, suboptimal uptake of safe and efficacious vaccines that already exist suggest that HIV vaccine acceptability cannot be assumed, particularly among communities most vulnerable to HIV. The present study aimed to identify barriers and motivators to future HIV vaccine acceptability among low socioeconomic, ethnically diverse men and women in Los Angeles County. Participants completed a cross-sectional survey assessing their attitudes and beliefs regarding future HIV vaccines. Hypothetical HIV vaccine scenarios were administered to determine HIV vaccine acceptability. Two-sided t-tests were performed, stratified by gender, to examine the association between vaccine acceptability and potential barriers and motivators. Barriers to HIV vaccine acceptability differed between men and women. For women, barriers to HIV vaccine acceptability were related to their intimate relationships (pnegative experiences with health care providers (pGender-specific interventions may increase vaccine acceptability among men and women at elevated risk for HIV infection. Among women, interventions need to focus on addressing barriers due to gendered power dynamics in relationships and discrimination in health care. Among men, education that addresses fears and misconceptions about adverse effects of HIV vaccination on health and the importance of vaccination as one component of integrated HIV prevention may increase vaccine acceptability. PMID:18484322

  16. What Is Mitochondrial DNA?

    ... DNA What is mitochondrial DNA? What is mitochondrial DNA? Although most DNA is packaged in chromosomes within ... proteins. For more information about mitochondria and mitochondrial DNA: Molecular Expressions, a web site from the Florida ...

  17. DNA and RNA sensor

    LIU; Tao; LIN; Lin; ZHAO; Hong; JIANG; Long

    2005-01-01

    This review summarizes recent advances in DNA sensor. Major areas of DNA sensor covered in this review include immobilization methods of DNA, general techniques of DNA detection and application of nanoparticles in DNA sensor.

  18. Who accepts responsibility for their transgressions?

    Schumann, Karina; Dweck, Carol S

    2014-12-01

    After committing an offense, transgressors can optimize their chances of reconciling with the victim by accepting responsibility. However, transgressors may be motivated to avoid admitting fault because it can feel threatening to accept blame for harmful behavior. Who, then, is likely to accept responsibility for a transgression? We examined how implicit theories of personality--whether people see personality as malleable (incremental theory) or fixed (entity theory)--influence transgressors' likelihood of accepting responsibility. We argue that incremental theorists may feel less threatened by accepting responsibility because they are more likely to view the situation as an opportunity for them to grow as a person and develop their relationship with the victim. We found support for our predictions across four studies using a combination of real-world and hypothetical offenses, and correlational and experimental methods. These studies therefore identify an important individual difference factor that can lead to more effective responses from transgressors. PMID:25252938

  19. DNA methylation regulates expression of VEGF-C, and S-adenosylmethionine is effective for VEGF-C methylation and for inhibiting cancer growth

    DNA hypomethylation may activate oncogene transcription, thus promoting carcinogenesis and tumor development. S-adenosylmethionine (SAM) is a methyl donor in numerous methylation reactions and acts as an inhibitor of intracellular demethylase activity, which results in hypermethylation of DNA. The main objectives of this study were to determine whether DNA hypomethylation correlated with vascular endothelial growth factor-C (VEGF-C) expression, and the effect of SAM on VEGF-C methylation and gastric cancer growth inhibition. VEGF-C expression was assayed by Western blotting and RT-qPCR in gastric cancer cells, and by immunohistochemistry in tumor xenografts. VEGF-C methylation was assayed by bisulfite DNA sequencing. The effect of SAM on cell apoptosis was assayed by flow cytometry analyses and its effect on cancer growth was assessed in nude mice. The VEGF-C promoters of MGC-803, BGC-823, and SGC-7901 gastric cancer cells, which normally express VEGF-C, were nearly unmethylated. After SAM treatment, the VEGF-C promoters in these cells were highly methylated and VEGF-C expression was downregulated. SAM also significantly inhibited tumor growth in vitro and in vivo. DNA methylation regulates expression of VEGF-C. SAM can effectively induce VEGF-C methylation, reduce the expression of VEGF-C, and inhibit tumor growth. SAM has potential as a drug therapy to silence oncogenes and block the progression of gastric cancer

  20. DNA methylation regulates expression of VEGF-C, and S-adenosylmethionine is effective for VEGF-C methylation and for inhibiting cancer growth

    Da, M.X. [Department of Surgical Oncology, Gansu Provincial Hospital, Lanzhou (China); Zhang, Y.B. [Department of Surgery, Ningxia Medical University, Yinchuan (China); Yao, J.B. [Department of Surgical Oncology, Gansu Provincial Hospital, Lanzhou (China); Duan, Y.X. [Department of Surgery, Ningxia Medical University, Yinchuan (China)

    2014-09-30

    DNA hypomethylation may activate oncogene transcription, thus promoting carcinogenesis and tumor development. S-adenosylmethionine (SAM) is a methyl donor in numerous methylation reactions and acts as an inhibitor of intracellular demethylase activity, which results in hypermethylation of DNA. The main objectives of this study were to determine whether DNA hypomethylation correlated with vascular endothelial growth factor-C (VEGF-C) expression, and the effect of SAM on VEGF-C methylation and gastric cancer growth inhibition. VEGF-C expression was assayed by Western blotting and RT-qPCR in gastric cancer cells, and by immunohistochemistry in tumor xenografts. VEGF-C methylation was assayed by bisulfite DNA sequencing. The effect of SAM on cell apoptosis was assayed by flow cytometry analyses and its effect on cancer growth was assessed in nude mice. The VEGF-C promoters of MGC-803, BGC-823, and SGC-7901 gastric cancer cells, which normally express VEGF-C, were nearly unmethylated. After SAM treatment, the VEGF-C promoters in these cells were highly methylated and VEGF-C expression was downregulated. SAM also significantly inhibited tumor growth in vitro and in vivo. DNA methylation regulates expression of VEGF-C. SAM can effectively induce VEGF-C methylation, reduce the expression of VEGF-C, and inhibit tumor growth. SAM has potential as a drug therapy to silence oncogenes and block the progression of gastric cancer.

  1. Influence of cytosine methylation on ultraviolet-induced cyclobutane pyrimidine dimer formation in genomic DNA

    Rochette, Patrick J. [Division of Pathology, Department of Medical Biology, Universite Laval, Quebec, QC (Canada); Division of Genetics, Department of Pediatrics, Faculty of Medicine and Health Sciences, Universite de Sherbrooke, Sherbrooke, QC (Canada); Department of Therapeutic Radiology, Yale School of Medicine, New Haven, CT (United States); Lacoste, Sandrine [Division of Pathology, Department of Medical Biology, Universite Laval, Quebec, QC (Canada); Division of Genetics, Department of Pediatrics, Faculty of Medicine and Health Sciences, Universite de Sherbrooke, Sherbrooke, QC (Canada); Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, MB (Canada); Therrien, Jean-Philippe [Division of Pathology, Department of Medical Biology, Universite Laval, Quebec, QC (Canada); Bastien, Nathalie [Division of Pathology, Department of Medical Biology, Universite Laval, Quebec, QC (Canada); Division of Genetics, Department of Pediatrics, Faculty of Medicine and Health Sciences, Universite de Sherbrooke, Sherbrooke, QC (Canada); Brash, Douglas E. [Department of Therapeutic Radiology, Yale School of Medicine, New Haven, CT (United States); Drouin, Regen, E-mail: Regen.Drouin@USherbrooke.ca [Division of Pathology, Department of Medical Biology, Universite Laval, Quebec, QC (Canada); Division of Genetics, Department of Pediatrics, Faculty of Medicine and Health Sciences, Universite de Sherbrooke, Sherbrooke, QC (Canada)

    2009-06-01

    The ultraviolet (UV) component of sunlight is the main cause of skin cancer. More than 50% of all non-melanoma skin cancers and >90% of squamous cell carcinomas in the US carry a sunlight-induced mutation in the p53 tumor suppressor gene. These mutations have a strong tendency to occur at methylated cytosines. Ligation-mediated PCR (LMPCR) was used to compare at nucleotide resolution DNA photoproduct formation at dipyrimidine sites either containing or lacking a methylated cytosine. For this purpose, we exploited the fact that the X chromosome is methylated in females only on the inactive X chromosome, and that the FMR1 (fragile-X mental retardation 1) gene is methylated only in fragile-X syndrome male patients. Purified genomic DNA was irradiated with UVC (254 nm), UVB (290-320 nm) or monochromatic UVB (302 and 313 nm) to determine the effect of different wavelengths on cyclobutane pyrimidine dimer (CPD) formation along the X-linked PGK1 (phosphoglycerate kinase 1) and FMR1 genes. We show that constitutive methylation of cytosine increases the frequency of UVB-induced CPD formation by 1.7-fold, confirming that methylation per se is influencing the probability of damage formation. This was true for both UVB sources used, either broadband or monochromatic, but not for UVC. Our data prove unequivocally that following UVB exposure methylated cytosines are significantly more susceptible to CPD formation compared with unmethylated cytosines.

  2. Influence of cytosine methylation on ultraviolet-induced cyclobutane pyrimidine dimer formation in genomic DNA

    The ultraviolet (UV) component of sunlight is the main cause of skin cancer. More than 50% of all non-melanoma skin cancers and >90% of squamous cell carcinomas in the US carry a sunlight-induced mutation in the p53 tumor suppressor gene. These mutations have a strong tendency to occur at methylated cytosines. Ligation-mediated PCR (LMPCR) was used to compare at nucleotide resolution DNA photoproduct formation at dipyrimidine sites either containing or lacking a methylated cytosine. For this purpose, we exploited the fact that the X chromosome is methylated in females only on the inactive X chromosome, and that the FMR1 (fragile-X mental retardation 1) gene is methylated only in fragile-X syndrome male patients. Purified genomic DNA was irradiated with UVC (254 nm), UVB (290-320 nm) or monochromatic UVB (302 and 313 nm) to determine the effect of different wavelengths on cyclobutane pyrimidine dimer (CPD) formation along the X-linked PGK1 (phosphoglycerate kinase 1) and FMR1 genes. We show that constitutive methylation of cytosine increases the frequency of UVB-induced CPD formation by 1.7-fold, confirming that methylation per se is influencing the probability of damage formation. This was true for both UVB sources used, either broadband or monochromatic, but not for UVC. Our data prove unequivocally that following UVB exposure methylated cytosines are significantly more susceptible to CPD formation compared with unmethylated cytosines.

  3. Monitoring device acceptance in implantable cardioverter defibrillator patients using the Florida Patient Acceptance Survey

    Versteeg, Henneke; Starrenburg, Annemieke; Denollet, Johan;

    2012-01-01

    Patient device acceptance might be essential in identifying patients at risk for adverse patient-reported outcomes following implantation of an implantable cardioverter defibrillator (ICD). We examined the validity and reliability of the Florida Patient Acceptance Scale (FPAS) and identified...

  4. p53-dependent activation of microRNA-34a in response to etoposide-induced DNA damage in osteosarcoma cell lines not impaired by dominant negative p53 expression.

    Chiara Novello

    Full Text Available Osteosarcoma (OS is the most common primary malignant bone tumor and prevalently occurs in the second decade of life. Etoposide, a chemotherapeutic agent used in combined treatments of recurrent human OS, belongs to the topoisomerase inhibitor family and causes DNA breakage. In this study we evaluated the cascade of events determined by etoposide-induced DNA damage in OS cell lines with different p53 status focusing on methylation status and expression of miR-34a that modulate tumor cell growth and cell cycle progression. Wild-type p53 U2-OS cells and U2-OS cells expressing dominant-negative form of p53 (U2- OS175 were more sensitive to etoposide than p53-deficient MG63 and Saos-2 cells, showing increased levels of unmethylated miR-34a, reduced expression of CDK4 and cell cycle arrest in G1 phase. In contrast, MG63 and Saos-2 cell lines presented aberrant methylation of miR-34a promoter gene with no miR-34a induction after etoposide treatment, underlining the close connection between p53 expression and miR-34a methylation status. Consistently, in p53siRNA transfected U2-OS cells we observed loss of miR-34a induction after etoposide exposure associated with a partial gain of gene methylation and cell cycle progress towards G2/M phase. Our results suggest that the open and unmethylated conformation of the miR-34a gene may be regulated by p53 able to bind the gene promoter. In conclusion, cell response to etoposide-induced DNA damage was not compromised in cells with dominant-negative p53 expression.

  5. DNA nanotechnology

    Nadrian C Seeman

    2003-01-01

    We are all aware that the DNA found in cells is a double helix consisting of two antiparallel strands held together by specific hydrogen-bonded base pairs; adenine (A always pairs with thymine (T, and guanine (G always pairs with cytosine (C. The specificity of this base pairing and the ability to ensure that it occurs in this fashion (and not some other1 is key to the use of DNA in materials applications. The double helical arrangement of the two molecules leads to a linear helix axis, linear not in the geometrical sense of being a straight line, but in the topological sense of being unbranched. Genetic engineers discovered in the 1970s how to splice together pieces of DNA to add new genes to DNA molecules2, and synthetic chemists worked out convenient syntheses for short pieces of DNA (up to ∼100–150 units in the 1980s3. Regardless of the impact of these technologies on biological systems, hooking together linear molecules leads only to longer linear molecules, with circles, knots, and catenanes perhaps resulting from time to time.

  6. Understanding social acceptance of electricity generation sources

    Social acceptability is a determinant factor in the failure or success of the government's decisions about which electricity generation sources will satisfy the growing demand for energy. The main goal of this study was to validate a causal trust-acceptability model for electricity generation sources. In the model, social acceptance of an energy source is directly caused by perceived risk and benefit and also by social trust in regulatory agencies (both directly and indirectly, through perceived risk and benefit). Results from a web-based survey of Chilean university students demonstrated that data for energy sources that are controversial in Chilean society (fossil fuels, hydro, and nuclear power) fit the hypothesized model, whereas data for non conventional renewable energy sources (solar, wind, geothermal and tidal) did not. Perceived benefit had the greatest total effect on acceptability, thus emerging as a key predictive factor of social acceptability of controversial electricity generation sources. Further implications for regulatory agencies are discussed. - Highlights: ► We tested a causal trust-acceptability model for electricity generation sources in Chile. ► Data for controversial energy sources in the Chilean society (fossil fuels, hydro and nuclear power) fit the hypothesized model. ► Data for non conventional renewable energy sources did not fit the data. ► Perceived benefit showed the greatest total effect on acceptability.

  7. Specific siRNA Downregulated TLR9 and Altered Cytokine Expression Pattern in Macrophage after CpG DNA Stimulation

    Bin Qiao; Baohua Li; Xiuli Yang; Hongyong Zhang; Yiwei Chu; Ying Wang; Sidong Xiong

    2005-01-01

    Bacterial CpG DNA or synthetic oligonucleotides (ODNs) that contain unmethylated CpG motifs (CpG ODN) can directly activate antigen-presenting cells (APCs) to secrete various cytokines through the intracellular receptor TLR9. Cytokine profiles elicited by the actions of stimulatory CpG DNA on TLR9 expressed APCs are crucial to the subsequent immune responses. To date, cytokine profiles in APCs upon CpG ODN stimulation in vitro are not fully investigated. In the present study, vector-based siRNA was used to downregulate TLR9 expression. Cytokine profiles were observed in murine macrophage cell line RAW264.7 transfected with TLR9-siRNA plasmid upon CpG ODN stimulation. We found that not all the cytokine expressions by the macrophage were decreased while TLR9 was downregulated. IL-12, TNF-α, IFN-γ and IL-1β expressions were significantly decreased, but IL-6,IFN-β and IL-10 expressions were not affected. Interestingly, the level of IFN-α was even increased. This alteration of cytokines produced by TLR9-downregulated APCs upon CpG ODN stimulation might indicate that the role of CpG DNA is more complicated in the pathogenesis and prevention of diseases. Cellular & Molecular Immunology.2005;2(2):130-135.

  8. Involvement of aberrant DNA methylation on reduced expression of lysophosphatidic acid receptor-1 gene in rat tumor cell lines

    Lysophosphatidic acid (LPA) is a bioactive phospholipid that stimulates cell proliferation, migration, and protects cells from apoptosis. It interacts with specific G protein-coupled transmembrane receptors. Recently, it has been reported that alterations of LPA receptor expression might be important in the malignant transformation of tumor cells. Therefore, to assess an involvement of DNA methylation in reduced expression of the LPA receptor-1 (lpa1) gene, we investigated the expression of the lpa1 gene and its DNA methylation patterns in rat tumor cell lines. Both rat brain-derived neuroblastoma B103 and liver-derived hepatoma RH7777 cells used in this study indicated no expression of lpa1. For the analysis of methylation status, bisulfite sequencing was performed with B103 and RH7777 cells, comparing with other lpa1 expressed cells and normal tissues of brain and liver. The lpa1 expressed cells and tissues were all unmethylated in this region of lpa1. In contrast, both B103 and RH7777 cells were highly methylated, correlating with reduced expression of the lpa1. Treatment with 5-aza 2'-deoxycytidine induced expression of lpa1 gene in B103 and RH7777 cells after 24 h. In RH7777 cells treated with 5-aza 2'-deoxycytidine, stress fiber formation was also observed in response to LPA in RH7777 cells, but not in untreated RH7777 cells. These results suggest that aberrant DNA methylation of the lpa1 gene may be involved in its reduced expression in rat tumor cells

  9. Specific siRNA Downregulated TLR9 and Altered Cytokine Expression Pattern in Macrophage after CpG DNA Stimulation

    BinQiao; BaohuaLi; XiuliYang; HongyongZhang; YiweiChu; YingWang; SidongXiong

    2005-01-01

    Bacterial CpG DNA or synthetic oligonucleotides (ODNs) that contain unmethylated CpG motifs (CpG ODN) can directly activate antigen-presenting cells (APCs) to secrete various cytokines through the intraceilular receptor TL R9. Cytokine profiles elicited by the actions of stimulatory CpG DNA on TLR9 expressed APCs are crucial to the subsequent immune responses. To date, cytokine profiles in APCs upon CpG ODN stimulation in vitro are not fully investigated. In the present study, vector-based siRNA was used to downregulate TLR9 expression. Cytokine profiles were observed in murine macrophage cell line RAW264.7 transfected with TLR9-siRNA plasmid uponCpG ODN stimulation. We found that not all the cytokine expressions by the macrophage were decreased whileTLR9 was downregulated. IL-12, TNF-α, IFN-γ and IL-1β expressions were significantly decreased, but IL-6, IFN-β and IL-10 expressions were not affected. Interestingly, the level of IFN-α was even increased. This alteration of cytokines produced by TLR9-downregulated APCs upon CpG ODN stimulation might indicate that the role of CpG DNA is more complicated in the pathogenesis and prevention of diseases. Cellular & Molecular Immunology. 2005;2(2):130-135.

  10. NEVADA TEST SITE WASTE ACCEPTANCE CRITERIA

    This document establishes the U. S. Department of Energy, National Nuclear Security Administration Nevada Site Office (NNSA/NSO) waste acceptance criteria (WAC). The WAC provides the requirements, terms, and conditions under which the Nevada Test Site will accept low-level radioactive and mixed waste for disposal. Mixed waste generated within the State of Nevada by NNSA/NSO activities is accepted for disposal. It includes requirements for the generator waste certification program, characterization, traceability, waste form, packaging, and transfer. The criteria apply to radioactive waste received at the Nevada Test Site Area 3 and Area 5 Radioactive Waste Management Site for storage or disposal

  11. Cone penetrometer moisture probe acceptance test report

    This Acceptance Test Report (ATR) documents the results of WHC-SD-WM-ATP-146 (Prototype Cone Penetrometer Moisture Probe Acceptance Test Procedure) and WHC-SD-WM-ATP-145 (Cone Penetrometer Moisture Probe Acceptance Test Procedure). The master copy of WHC-SD-WM-ATP-145 can be found in Appendix A and the master copy of WHC-SD-WM-ATP-146 can be found in Appendix B. Also included with this report is a matrix showing design criteria of the cone penetrometer moisture probe and the verification method used (Appendix C)

  12. Hanford Site Solid Waste Acceptance Criteria

    This manual defines the Hanford Site radioactive, hazardous, and sanitary solid waste acceptance criteria. Criteria in the manual represent a guide for meeting state and federal regulations; DOE Orders; Hanford Site requirements; and other rules, regulations, guidelines, and standards as they apply to acceptance of radioactive and hazardous solid waste at the Hanford Site. It is not the intent of this manual to be all inclusive of the regulations; rather, it is intended that the manual provide the waste generator with only the requirements that waste must meet in order to be accepted at Hanford Site TSD facilities

  13. The Acceptability of Speech with Radio Interference

    Baykaner, K.; Hummersone, H.; Mason, R.;

    2014-01-01

    A listening test was conducted to investigate the acceptability of audio-on-audio interference for radio programs featuring speech as the target. Twenty-one subjects, including naïve and expert listeners, were presented with 200 randomly assigned pairs of stimuli and asked to report, for each trial......, whether the listening scenario was acceptable or unacceptable. Stimuli pairs were set to randomly selected SNRs ranging from 0 to 45 dB. Results showed no significant difference between subjects according to listening experience. A logistic regression to acceptability was carried out based on SNR. The...

  14. Approaches to acceptable risk: a critical guide

    Fischhoff, B.; Lichtenstein, S.; Slovic, P.; Keeney, R.; Derby, S.

    1980-12-01

    Acceptable-risk decisions are an essential step in the management of technological hazards. In many situations, they constitute the weak (or missing) link in the management process. The absence of an adequate decision-making methodology often produces indecision, inconsistency, and dissatisfaction. The result is neither good for hazard management nor good for society. This report offers a critical analysis of the viability of various approaches as guides to acceptable-risk decisions. This report seeks to define acceptable-risk decisions and to examine some frequently proposed, but inappropriate, solutions. 255 refs., 22 figs., 25 tabs.

  15. Hanford Site Solid Waste Acceptance Criteria

    1993-11-17

    This manual defines the Hanford Site radioactive, hazardous, and sanitary solid waste acceptance criteria. Criteria in the manual represent a guide for meeting state and federal regulations; DOE Orders; Hanford Site requirements; and other rules, regulations, guidelines, and standards as they apply to acceptance of radioactive and hazardous solid waste at the Hanford Site. It is not the intent of this manual to be all inclusive of the regulations; rather, it is intended that the manual provide the waste generator with only the requirements that waste must meet in order to be accepted at Hanford Site TSD facilities.

  16. NEVADA TEST SITE WASTE ACCEPTANCE CRITERIA

    U.S. DEPARTMENT OF ENERGY, NATIONAL NUCLEAR SECURITY ADMINISTRATION, NEVADA SITE OFFICE

    2005-07-01

    This document establishes the U. S. Department of Energy, National Nuclear Security Administration Nevada Site Office (NNSA/NSO) waste acceptance criteria (WAC). The WAC provides the requirements, terms, and conditions under which the Nevada Test Site will accept low-level radioactive and mixed waste for disposal. Mixed waste generated within the State of Nevada by NNSA/NSO activities is accepted for disposal. It includes requirements for the generator waste certification program, characterization, traceability, waste form, packaging, and transfer. The criteria apply to radioactive waste received at the Nevada Test Site Area 3 and Area 5 Radioactive Waste Management Site for storage or disposal.

  17. DNA nanotechnology

    Seeman, Nadrian C.

    2003-01-01

    Since Watson and Crick’s determination of its structure nearly 50 years ago, DNA has come to fill our lives in many areas, from genetic counseling to forensics, from genomics to gene therapy. These, and other ways in which DNA affects human activities, are related to its function as genetic material, not just our genetic material, but the genetic material of all living organisms. Here, we will ignore DNA’s biological role; rather, we will discuss how the properties that make it so successful ...

  18. Cultural Technology Acceptance Model for Consumers’ Acceptance of Arabic E-Commerce Websites

    Omar (Mohammad Ali Al-Qudah

    2014-02-01

    Full Text Available The aim of this study is to identify the factors affecting consumer’s acceptance of Arabic e-commerce websites and to propose a cultural technology acceptance model for consumer’s acceptance of Arabic e-commerce websites. The proposed model was built based on, technology acceptance model, trust, perceived risk and Hofstede’s cultural values as moderators between trust and perceived risk and purchase intention. In addition, Arabic language from literature review was added. Based on these theories and concepts, this study identifies the factors affecting consumer’s acceptance of Arabic e-commerce websites.

  19. Integrated Model for E-Learning Acceptance

    Ramadiani; Rodziah, A.; Hasan, S. M.; Rusli, A.; Noraini, C.

    2016-01-01

    E-learning is not going to work if the system is not used in accordance with user needs. User Interface is very important to encourage using the application. Many theories had discuss about user interface usability evaluation and technology acceptance separately, actually why we do not make it correlation between interface usability evaluation and user acceptance to enhance e-learning process. Therefore, the evaluation model for e-learning interface acceptance is considered important to investigate. The aim of this study is to propose the integrated e-learning user interface acceptance evaluation model. This model was combined some theories of e-learning interface measurement such as, user learning style, usability evaluation, and the user benefit. We formulated in constructive questionnaires which were shared at 125 English Language School (ELS) students. This research statistics used Structural Equation Model using LISREL v8.80 and MANOVA analysis.

  20. Gas characterization system software acceptance test report

    This document details the results of software acceptance testing of gas characterization systems. The gas characterization systems will be used to monitor the vapor spaces of waste tanks known to contain measurable concentrations of flammable gases

  1. Gas characterization system software acceptance test procedure

    This document details the Software Acceptance Testing of gas characterization systems. The gas characterization systems will be used to monitor the vapor spaces of waste tanks known to contain measurable concentrations of flammable gases

  2. Synthetic DNA

    O’ Driscoll, Aisling; Sleator, Roy D.

    2013-01-01

    With world wide data predicted to exceed 40 trillion gigabytes by 2020, big data storage is a very real and escalating problem. Herein, we discuss the utility of synthetic DNA as a robust and eco-friendly archival data storage solution of the future.

  3. DNA Investigations.

    Mayo, Ellen S.; Bertino, Anthony J.

    1991-01-01

    Presents a simulation activity that allow students to work through the exercise of DNA profiling and to grapple with some analytical and ethical questions involving a couple arranging with a surrogate mother to have a baby. Can be used to teach the principles of restriction enzyme digestion, gel electrophoresis, and probe hybridization. (MDH)

  4. Acceptance Test Plan for ANSYS Software

    This plan governs the acceptance testing of the ANSYS software (Full Mechanical Release 5.5) for use on Project Word Management Contract (PHMC) computer systems (either UNIX or Microsoft Windows/NT). There are two phases to the acceptance testing covered by this test plan: program execution in accordance with the guidance provided in installation manuals; and ensuring results of the execution are consistent with the expected physical behavior of the system being modeled

  5. Functional food acceptance in the food chain

    Krutulyte, Rasa

    2010-01-01

    This thesis analyses consumer acceptance of functional foods and food manufacturers' decision to develop functional foods. The thesis sets up four key research questions: (1) How consumers accept functional foods enriched with omega-3? (2) How the intention of purchasing carrier ingredient combinations is explained by general attitudes to functional foods, consumers' health concerns, and the perceived fit of a particular carrier ingredient combination? (3) How food manufacturers decide to dev...

  6. Anticipating Public Acceptance: The Hydrogen Case

    Di Ruggero, O.

    2014-01-01

    Current energy related issues, such as climate change or the oil depletion, demand technological and societal change towards new ways of producing and using energy. One of the challenges coming with the development of these new solutions relates to the impact that these technologies will have on society, and how people will react to these technologies. This phenomenon is often referred to as public acceptance. The relevance of considering public acceptance of emerging technologies is self-evi...

  7. Acceptance of Diabetes Education via the Telephone

    Boren, Suzanne Austin; De Leo, Gianluca; Krishna, Santosh

    2005-01-01

    Patients require education and information as they engage in self-help, self-care, and disease management activities. The objective of this study was to explore the acceptance of voice technologies in diabetes patient education. Eighteen participants listened to a total of 324 diabetes educational messages and were provided the opportunity to rate the usefulness of each message. This study demonstrated that brief telephone-based education can be acceptable and useful.

  8. Acceptance testing of photographic film dosemeters

    The reports describes a system for acceptance testing of photographic film dosemeters in customary use in personal dosimetry. The system comprises a test of some of the fundamental dosimetric properties of the film. The acceptance test will be performed in connection with the semi-annual purchases of dosemeter film to the National Institute of Radiation Hygiene. The test program comprises both constancy tests and absolute requirements. The latter refer mainly to ISO 1757 - Personal photographic dosemeters. 2 refs

  9. Schematic effects of rape myth acceptance

    Süssenbach, Philipp

    2011-01-01

    In a set of studies, the present work investigates the type of influence exerted by rape myth acceptance (RMA). Rape myths may be defined as "beliefs about rape (i.e., about its causes, context, consequences, perpetrators, victims, and their interaction) that serve to deny, downplay or justify male sexual aggression against women" (Gerger, Kley, Bohner, & Siebler, 2007, p. 423). In this work the hypothesis is made and tested that the acceptance of rape myths influences the processing of relev...

  10. Risk aversion in risk acceptance criteria

    The risk averse attitude that is included in some proposed risk acceptance criteria is examined. It is shown that it is a weaker attitude than risk aversion, as is commonly defined in decision theory. Consequently, the boundary curve separating acceptable and unacceptable regions does not have to be a straight line on the logarithmic frequency-consequence space. A curve of variable slope would express the same attitude as long as the slope is less than -1

  11. A structural model of technology acceptance

    Etienne Erasmus; Sebastiaan Rothmann; Chrizanne van Eeden

    2015-01-01

    Orientation: Enterprise resource systems have not always led to significant organisational enhancement and many projects in which these systems have been implemented turn out to be over budget, not on time and unsuccessful.Research purpose: The aim of this study was to test the technology acceptance model within a South African SAP® Enterprise Resource Planning user environment.Motivation for the study: No study could be traced in which the technology acceptance model has been evaluated in th...

  12. Accepting the truth: A step toward reconciliation

    Ilić Zoran

    2002-01-01

    The truth is not only about accepting the facts. The truth includes emotional components - longing for acknowledgment of mistakes and validation of painful losses and experiences. While one side in the conflict considers itself as the only victim and experiences determination and acknowledgment of the truth as a possibility for healing its own trauma through satisfaction of justice and compensation, the other side is not accepting the truth. For this other side, confrontation with the facts i...

  13. Customer acceptance of internet banking in Estonia

    Eriksson, Kent; Kerem, Katri; Nilsson, Daniel

    2005-01-01

    Purpose – The purpose of this paper is to study technology acceptance of internet banking inEstonia, an emerging east European economy. Design/methodology/approach – The present paper modifies the technology acceptance modeland applies it to bank customers in Estonia, because Estonia, a country with a developing economy, has focused on internet banking as an important distribution channel. Findings – The findings suggest that internet bank use increases insofar as customers perceive it as use...

  14. Polymer microspheres carrying fluorescent DNA probes

    Chen, Xiaoyu; Dai, Zhao; Zhang, Jimei; Xu, Shichao; Wu, Chunrong; Zheng, Guo

    2010-07-01

    A polymer microspheres carried DNA probe, which was based on resonance energy transfer, was presented in this paper when CdTe quantum dots(QDs) were as energy donors, Au nanoparticles were as energy accepters and poly(4- vinylpyrindine-co-ethylene glycol dimethacrylate) microspheres were as carriers. Polymer microspheres with functional group on surfaces were prepared by distillation-precipitation polymerization when ethylene glycol dimethacrylate was as crosslinker in acetonitrile. CdTe QDs were prepared when 3-mercaptopropionic acid(MPA) was as the stabilizer in aqueous solution. Because of the hydrogen-bonding between the carboxyl groups of MPA on QDs and the pyrindine groups on the microspheres, the QDs were self-assembled onto the surfaces of microspheres. Then, the other parts of DNA probe were finished according to the classic method. The DNA detection results indicated that this novel fluorescent DNA probe system could recognize the existence of complementary target DNA or not.

  15. A possible role of repair proteins BRCA1 and DNA-PK in the processing of oxidative DNA damage

    Alexandros G Georgakilas

    2008-08-01

    Full Text Available BRCA1 and DNA-PK are two significant multifunctional proteins involved primarily in the processing of double strand breaks (DSBs. BRCA1 participates actively in homologous recombination (HR while DNA-PK in non-homologous end joining (NHEJ. In this mini review, we discuss all recent evidence for a possible involvement of these repair proteins also in the processing of oxidatively-induced DNA damage.Keyword: DNA damage, BRCA1, DNA-PKReceived: 6 June 2008, Accepted: 10 August 2008 Published online: 18 August 2008

  16. DNA Repair by Reversal of DNA Damage

    Yi, Chengqi; He, Chuan

    2013-01-01

    Endogenous and exogenous factors constantly challenge cellular DNA, generating cytotoxic and/or mutagenic DNA adducts. As a result, organisms have evolved different mechanisms to defend against the deleterious effects of DNA damage. Among these diverse repair pathways, direct DNA-repair systems provide cells with simple yet efficient solutions to reverse covalent DNA adducts. In this review, we focus on recent advances in the field of direct DNA repair, namely, photolyase-, alkyltransferase-,...

  17. Flexibility of the genetic code with respect to DNA structure

    Baisnée, P. F.; Baldi, Pierre; Brunak, Søren;

    2001-01-01

    Motivation. The primary function of DNA is to carry genetic information through the genetic code. DNA, however, contains a variety of other signals related, for instance, to reading frame, codon bias, pairwise codon bias, splice sites and transcription regulation, nucleosome positioning and DNA...... structure. Here we study the relationship between the genetic code and DNA structure and address two questions. First, to which degree does the degeneracy of the genetic code and the acceptable amino acid substitution patterns allow for the superimposition of DNA structural signals to protein coding...... sequences? Second, is the origin or evolution of the genetic code likely to have been constrained by DNA structure? Results. We develop an index for code flexibility with respect to DNA structure. Using five different di- or tri-nucleotide models of sequence-dependent DNA structure, we show that the...

  18. Public acceptance activities by the Rokkasho project

    Japan Nuclear Fuel Limited, incorporated by mainly ten Japanese utilities, engages in constructing and operating nuclear fuel cycle facilities such as reprocessing, uranium enrichment and low level radioactive waste disposal at Rokkasho village in Aomori prefecture, 600 kilometers north of Tokyo, where is the most northern part of Honshu island. This presentation deals with the situation concerning the Rokkasho project and our public acceptance activities. The expansion of anti nuclear movement was spread not only Aomori prefecture but also all over Japan, affected strongly by the Chernobyl accident. In 1988,16 anti-nuclear groups including labor-union organized a committee for the purpose of a campaign to collect signatures for opposing nuclear fuel cycle facilities. Those groups brought in a lawsuit against the Japan Nuclear Fuel Limited, for stopping the construction of enrichment plant. Facing furious anti nuclear fuel cycle movements in Aomori prefecture, the Federation of Electric Power Companies decided to re strengthen the public acceptance activities and established the Nuclear Fuel Joint head office together with Japan Nuclear Fuel Service Co.and Japan Nuclear Fuel Industry Co. To this new organization many excellent members were dispatched from all electric power companies. The target area for public acceptance activities expanded from the surrounding area to all area of Aomori prefecture. The first policy of public acceptance activities is 'Appealing by face to face' which means the direct personal contact with people being anxious about nuclear fuel cycle facilities. The second main policy is 'Seeing the plant itself is better than persuading orally.' Survey conducted by a newspaper company in respect with the public acceptance of Rokkasho project in July 1989 July 1992 showed a favorable change of the public acceptance. However, one also has to recognize that most of people still have anxiety about the nuclear fuel cycle facilities and we shall

  19. DNA-inorganic hybrid nanovaccine for cancer immunotherapy

    Zhu, Guizhi; Liu, Yijing; Yang, Xiangyu; Kim, Young-Hwa; Zhang, Huimin; Jia, Rui; Liao, Hsien-Shun; Jin, Albert; Lin, Jing; Aronova, Maria; Leapman, Richard; Nie, Zhihong; Niu, Gang; Chen, Xiaoyuan

    2016-03-01

    Cancer evolves to evade or compromise the surveillance of the immune system, and cancer immunotherapy aims to harness the immune system in order to inhibit cancer development. Unmethylated CpG dinucleotide-containing oligonucleotides (CpG), a class of potent adjuvants that activate the toll-like receptor 9 (TLR9) located in the endolysosome of many antigen-presenting cells (APCs), are promising for cancer immunotherapy. However, clinical application of synthetic CpG confronts many challenges such as suboptimal delivery into APCs, unfavorable pharmacokinetics caused by limited biostability and short in vivo half-life, and side effects associated with leaking of CpG into the systemic circulation. Here we present DNA-inorganic hybrid nanovaccines (hNVs) for efficient uptake into APCs, prolonged tumor retention, and potent immunostimulation and cancer immunotherapy. hNVs were self-assembled from concatemer CpG analogs and magnesium pyrophosphate (Mg2PPi). Mg2PPi renders hNVs resistant to nuclease degradation and thermal denaturation, both of which are demanding characteristics for effective vaccination and the storage and transportation of vaccines. Fluorophore-labeled hNVs were tracked to be efficiently internalized into the endolysosomes of APCs, where Mg2PPi was dissolved in an acidic environment and thus CpG analogs were exposed to hNVs. Internalized hNVs in APCs led to (1) elevated secretion of proinflammatory factors, and (2) elevated expression of co-stimulatory factors. Compared with molecular CpG, hNVs dramatically prolonged the tissue retention of CpG analogs and reduced splenomegaly, a common side effect of CpG. In a melanoma mouse model, two injections of hNVs significantly inhibited the tumor growth and outperformed the molecular CpG. These results suggest hNVs are promising for cancer immunotherapy.Cancer evolves to evade or compromise the surveillance of the immune system, and cancer immunotherapy aims to harness the immune system in order to inhibit

  20. DNA Methylation

    İzmirli, Müzeyyen; Tufan, Turan; Alptekin, Davut

    2012-01-01

    Methylation is a chemical reaction in biological systems for normal genome regulation and development. It is a well known type of epigenetic mechanism. Methylation which regulates gene expression via epigenetic events like gene activation, repression, and chromatin remodelling, consists of two methylation systems. One of these systems is DNA methylation whereas the other is protein (histone) methylation. These systems are associated with some fundamental abnormalities and diseases. This revi...

  1. DNA Nanorobotics

    Hamdi M; Ferreira A

    2006-01-01

    This paper presents a molecular mechanics study for new nanorobotic structures using molecular dynamics (MD) simulations coupled to virtual reality (VR) techniques. The operator can design and characterize through molecular dynamics simulation the behavior of bionanorobotic components and structures through 3-D visualization. The main novelty of the proposed simulations is based on the mechanical characterization of passive/active robotic devices based on double stranded DNA molecules. Their ...

  2. DNA Methylation

    Muzeyyen Izmirli; Turan Tufan; Davut Alptekin

    2012-01-01

    Methylation is a chemical reaction in biological systems for normal genome regulation and development. It is a well known type of epigenetic mechanism. Methylation which regulates gene expression via epigenetic events like gene activation, repression, and chromatin remodelling, consists of two methylation systems. One of these systems is DNA methylation whereas the other is protein (histone) methylation. These systems are associated with some fundamental abnormalities and diseases. This revie...

  3. Visualizing and Assessing Acceptance Sampling Plans: The R Package AcceptanceSampling

    Andreas Kiermeier

    2008-01-01

    Manufacturers and government agencies frequently use acceptance sampling to decide whether a lot from a supplier or exporting country should be accepted or rejected. International standards on acceptance sampling provide sampling plans for specific circumstances.The aim of this package is to provide an easy-to-use interface to visualize single, double or multiple sampling plans. In addition, methods have been provided to enable the user to assess sampling plans against pre-specified levels of...

  4. Physiologic correlates to background noise acceptance

    Tampas, Joanna; Harkrider, Ashley; Nabelek, Anna

    2001-05-01

    Acceptance of background noise can be evaluated by having listeners indicate the highest background noise level (BNL) they are willing to accept while following the words of a story presented at their most comfortable listening level (MCL). The difference between the selected MCL and BNL is termed the acceptable noise level (ANL). One of the consistent findings in previous studies of ANL is large intersubject variability in acceptance of background noise. This variability is not related to age, gender, hearing sensitivity, personality, type of background noise, or speech perception in noise performance. The purpose of the current experiment was to determine if individual differences in physiological activity measured from the peripheral and central auditory systems of young female adults with normal hearing can account for the variability observed in ANL. Correlations between ANL and various physiological responses, including spontaneous, click-evoked, and distortion-product otoacoustic emissions, auditory brainstem and middle latency evoked potentials, and electroencephalography will be presented. Results may increase understanding of the regions of the auditory system that contribute to individual noise acceptance.

  5. Acceptance of Swedish e-health services

    Mary-Louise Jung

    2010-11-01

    Full Text Available Mary-Louise Jung1, Karla Loria11Division of Industrial Marketing, e-Commerce and Logistics, Lulea University of Technology, SwedenObjective: To investigate older people’s acceptance of e-health services, in order to identify determinants of, and barriers to, their intention to use e-health.Method: Based on one of the best-established models of technology acceptance, Technology Acceptance Model (TAM, in-depth exploratory interviews with twelve individuals over 45 years of age and of varying backgrounds are conducted.Results: This investigation could find support for the importance of usefulness and perceived ease of use of the e-health service offered as the main determinants of people’s intention to use the service. Additional factors critical to the acceptance of e-health are identified, such as the importance of the compatibility of the services with citizens’ needs and trust in the service provider. Most interviewees expressed positive attitudes towards using e-health and find these services useful, convenient, and easy to use.Conclusion: E-health services are perceived as a good complement to traditional health care service delivery, even among older people. These people, however, need to become aware of the e-health alternatives that are offered to them and the benefits they provide.Keywords: health services, elderly, technology, Internet, TAM, patient acceptance, health-seeking behavior

  6. International acceptability of advanced safeguarding techniques

    There are active development programs now under way to significantly enhance the effectiveness of international safeguarding. Advanced safeguarding techniques now under development include new material accounting methods utilizing nondestructive assay techniques, more reliable surveillance instrumentation, tamper-resistant and tamper-indicating seals, new means of utilizing continuous human inspection, and systems that incorporate both passive and active use-denial technologies. Before these new safeguarding techniques are utilized, however, they must be acceptable to the international community. This will unquestionably result in a compromise between what is technically feasible and what is politically acceptable. This report highlights many of the elements common to advanced safeguarding techniques that impact directly upon international acceptability. The concept of acceptability is viewed from the perspective of three different groups: (1) those States seeking upgraded safeguards, (2) those States having safeguards imposed upon them, and (3) the International Atomic Energy Agency. In general, a more conducive climate exists today for the acceptance of advanced safeguarding techniques than at any period in the past; but the differences between advanced safeguards and those safeguards being employed today are so large that considerable opposition to their implementation can be expected

  7. Children acceptance of laser dental treatment

    Lazea, Andreea; Todea, Carmen

    2016-03-01

    Objectives: To evaluate the dental anxiety level and the degree of acceptance of laser assisted pedodontic treatments from the children part. Also, we want to underline the advantages of laser use in pediatric dentistry, to make this technology widely used in treating dental problems of our children patients. Methods: Thirty pediatric dental patients presented in the Department of Pedodontics, University of Medicine and Pharmacy "Victor Babeş", Timişoara were evaluated using the Wong-Baker pain rating scale, wich was administered postoperatory to all patients, to assess their level of laser therapy acceptance. Results: Wong-Baker faces pain rating scale (WBFPS) has good validity and high specificity; generally it's easy for children to use, easy to compare and has good feasibility. Laser treatment has been accepted and tolerated by pediatric patients for its ability to reduce or eliminate pain. Around 70% of the total sample showed an excellent acceptance of laser dental treatment. Conclusions: Laser technology is useful and effective in many clinical situations encountered in pediatric dentistry and a good level of pacient acceptance is reported during all laser procedures on hard and soft tissues.

  8. Electricity supply between acceptance, acceptability and social compatibility; Energieversorgung zwischen Akzeptanz, Akzeptabilitaet und Sozialvertraeglichkeit

    Schubert, Katharina; Koch, Marco K. [Bochum Univ. (Germany). Lehrstuhl Energiesysteme und Energiewirtschaft (LEE)

    2012-11-01

    Acceptance promotion is supposed to be an indispensable premise for a successful realization of an energy concept. The contribution identifies deficiencies of the energy policy, including intransparency, complexity of decision procedures, for instance in case of the so called energy transmission line extension acceleration law, that has caused irritation and anger in the public. The justification of acceptance promotion is questioned in connection with the German nuclear policy reversal following the Fukushima accident. A research program ''public acceptance of large-scale power plants for electricity generation'' is presented. The issues criteria and limits of acceptability are of main importance for this discussion.

  9. Improving acceptance in wind power planning

    Hammarlund, K. [University of Lund (Sweden). Dept. of Social and Economic Geography

    1996-12-31

    This paper presents important factors and planning procedures for public acceptance of wind power. Opinion surveys in Sweden show that acceptance is connected to the concept of utility rather than the aesthetic values. If wind turbines are confined by the authorities to marginal areas they will not earn their rightful place in the landscape by being of use. A positive attitude in general promotes positive experience of the effects of wind turbines. It is therefore essential to establish a sense of cooperation between the project management and the public. An open dialogue and continuous information will increase the possibilities for acceptance of future development. We must establish new codes of practice in permit processing because policies today contains idealogical and practical contradictions between the development and preservation of landscapes. (author)

  10. Improving acceptance in wind power planning

    This paper presents important factors and planning procedures for public acceptance of wind power. Opinion surveys in Sweden show that acceptance is connected to the concept of utility rather than the aesthetic values. If wind turbines are confined by the authorities to marginal areas they will not earn their rightful place in the landscape by being of use. A positive attitude in general promotes positive experience of the effects of wind turbines. It is therefore essential to establish a sense of cooperation between the project management and the public. An open dialogue and continuous information will increase the possibilities for acceptance of future development. We must establish new codes of practice in permit processing because policies today contains idealogical and practical contradictions between the development and preservation of landscapes. (author)

  11. Functional food acceptance in the food chain

    Krutulyte, Rasa

    This thesis analyses consumer acceptance of functional foods and food manufacturers' decision to develop functional foods. The thesis sets up four key research questions: (1) How consumers accept functional foods enriched with omega-3? (2) How the intention of purchasing carrier ingredient...... another central issue of the paper. Results revealed that the general attitudes towards functional foods are related to the purchase intention with regard to functional foods described by their carrier/ingredient combinations. Consumers' attitudes towards specific carrier ingredient combinations define...... to develop functional foods? Research question 1 The health benefit of a functional food product is in fact limited if it is not eaten regularly and thus is not part of the healthy diet. For that reason it is highly relevant to look at the dietary change research when studying functional food acceptance...

  12. Working Toward Public Acceptance in Germany

    West German utilities show a typical three level structure: local distributors, regional producers/distributors and some ten nationwide producers/distributors. Only the latter have the necessary financial and organizational means for running nuclear power stations. Public acceptance, on the other hand, does not care for state borders. Today, the leading German electronic media and the relevant print media are being distributed and received nationwide, transporting opinion as well as acceptance or non-acceptance. This paper describes the main projects of 'Nuclear Information Group' and the strategy considerations behind. Information centers at NPP sites can cover local information needs, regional utilities will cope with regional needs. It became obvious at the very beginning of the nuclear controversy, however, that it is also necessary to establish an additional, strictly federal, i. e. central, organisation that takes care of all nationwide information needs

  13. FFTBM and primary pressure acceptance criterion

    When thermalhydraulic computer codes are used for simulation in the area of nuclear engineering the question is how to conduct an objective comparison between the code calculation and measured data. To answer this the fast Fourier transform based method (FFTBM) was developed. When the FFTBM method was developed the acceptance criteria for primary pressure and total accuracy were set. In the recent study the FFTBM method was used for accuracy quantification of RD-14M large LOCA test B9401 calculations. The blind accuracy analysis indicated good total accuracy while the primary pressure criterion was not fulfilled. The objective of the study was therefore to investigate the reasons for not fulfilling the primary pressure acceptance criterion and the applicability of the criterion to experimental facilities simulating heavy water reactor. The results of the open quantitative analysis showed that sensitivity analysis for influence parameters provide sufficient information to judge in which calculation the accuracy of primary pressure is acceptable. (author)

  14. Issues affecting the acceptance of hydrogen fuel

    While the topic of hydrogen as an alternative vehicle fuel is gaining increasing attention internationally, one significant aspect of its introduction has been given less attention than others: the public acceptance of such a new technology and fuel. After reviewing the existing literature on acceptance, risk perception and customer satisfaction, this paper describes the development of a model that illustrates important aspects in influencing a person's attitude towards a new product. 'Values', 'wants' and 'perception' are the three components found to influence acceptance, they themselves are affected by 'social background' and 'experience'. Suggestions are then given on how to use marketing methods, education projects and product exposure in order to maximise the likelihood of a successful introduction of hydrogen as an alternative fuel. (author)

  15. Hanford Site solid waste acceptance criteria

    Order 5820.2A requires that each treatment, storage, and/or disposal facility (referred to in this document as TSD unit) that manages low-level or transuranic waste (including mixed waste and TSCA PCB waste) maintain waste acceptance criteria. These criteria must address the various requirements to operate the TSD unit in compliance with applicable safety and environmental requirements. This document sets forth the baseline criteria for acceptance of radioactive waste at TSD units operated by WMH. The criteria for each TSD unit have been established to ensure that waste accepted can be managed in a manner that is within the operating requirements of the unit, including environmental regulations, DOE Orders, permits, technical safety requirements, waste analysis plans, performance assessments, and other applicable requirements. Acceptance criteria apply to the following TSD units: the Low-Level Burial Grounds (LLBG) including both the nonregulated portions of the LLBG and trenches 31 and 34 of the 218-W-5 Burial Ground for mixed waste disposal; Central Waste Complex (CWC); Waste Receiving and Processing Facility (WRAP); and T Plant Complex. Waste from all generators, both from the Hanford Site and from offsite facilities, must comply with these criteria. Exceptions can be granted as provided in Section 1.6. Specific waste streams could have additional requirements based on the 1901 identified TSD pathway. These requirements are communicated in the Waste Specification Records (WSRds). The Hanford Site manages nonradioactive waste through direct shipments to offsite contractors. The waste acceptance requirements of the offsite TSD facility must be met for these nonradioactive wastes. This document does not address the acceptance requirements of these offsite facilities

  16. NEVADA TEST SITE WASTE ACCEPTANCE CRITERIA

    This document establishes the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office (NNSA/NSO) waste acceptance criteria (WAC). The WAC provides the requirements, terms, and conditions under which the Nevada Test Site (NTS) will accept low-level radioactive (LLW) and mixed waste (MW) for disposal. It includes requirements for the generator waste certification program, characterization, traceability, waste form, packaging, and transfer. The criteria apply to radioactive waste received at the NTS Area 3 and Area 5 Radioactive Waste Management Complex (RWMC) for storage or disposal

  17. Nevada Test Site Waste Acceptance Criteria

    This document establishes the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office (NNSA/NSO) waste acceptance criteria (WAC). The WAC provides the requirements, terms, and conditions under which the Nevada Test Site (NTS) will accept low-level radioactive (LLW) and mixed waste (MW) for disposal. It includes requirements for the generator waste certification program, characterization, traceability, waste form, packaging, and transfer. The criteria apply to radioactive waste received at the NTS Area 3 and Area 5 Radioactive Waste Management Complex (RWMC) for storage or disposal

  18. European consumers' acceptance of functional foods

    Grunert, Klaus G.

    2010-01-01

    Consumer acceptance of functional foods is analyzed from the perspective of consumer quality perception of food products. Four major dimensions of food quality are identified: taste and other sensory characteristics, healthiness, convenience, and naturalness. Functional foods provide, from the...... consumer perspective, synergies between healthiness and convenience, but may, in the consumer mind, lead to trade-offs between healthiness on the one side and taste and naturalness on the other side. This may explain the reluctance of European consumers to accept functional food products....

  19. Effective acceptance evaluation of linear resonance accelerator

    One of the most important challenges for accelerators is to match an accelerating beam with an accelerator acceptance. It allows one reduce a particle loss. Effective acceptance evaluation of linear resonance accelerator with RF focusing is carried out with no taking into account a beam space charge; a model taking into account non-coherent bunch particle oscillations is considered with the use of an averaging technique over rapid oscillations. Analytical results obtained are verified. Computer simulations of self-consistent low-energy ion beam dynamics are performed.

  20. Social acceptance of handicapped people in Mongolia

    Jargalmaa Bayarsaikhan

    2009-06-01

    Full Text Available The social integration of people with handicaps is an important social task. Their equal participation in social life cannot only be reached by law, but the acceptance of the social environment is an essential basic condition. Experiences, attitudes and behaviours of non-handicapped people towards people with handicaps are very important. The situation of people with handicaps has been confronted with a lack of understanding prejudices and refusal up to today. This study investigates how the mongolian citizens think about handicapped people. The adults were asked about subjects like social acceptance, refusal or even depreciation of people with handicaps.

  1. Nevada Test Site Waste Acceptance Criteria

    U. S. Department of Energy, National Nuclear Security Administration Nevada Site Office

    2005-10-01

    This document establishes the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office (NNSA/NSO) waste acceptance criteria (WAC). The WAC provides the requirements, terms, and conditions under which the Nevada Test Site (NTS) will accept low-level radioactive (LLW) and mixed waste (MW) for disposal. It includes requirements for the generator waste certification program, characterization, traceability, waste form, packaging, and transfer. The criteria apply to radioactive waste received at the NTS Area 3 and Area 5 Radioactive Waste Management Complex (RWMC) for storage or disposal.

  2. DNA repair

    In this chapter a series of DNA repair pathways are discussed which are available to the cell to cope with the problem of DNA damaged by chemical or physical agents. In the case of microorganisms our knowledge about the precise mechanism of each DNA repair pathway and the regulation of it has been improved considerably when mutants deficient in these repair mechanisms became available. In the case of mammalian cells in culture, until recently there were very little repair deficient mutants available, because in almost all mammalian cells in culture at least the diploid number of chromosomes is present. Therefore the frequency of repair deficient mutants in such populations is very low. Nevertheless because replica plating techniques are improving some mutants from Chinese hamsters ovary cells and L5178Y mouse lymphoma cells are now available. In the case of human cells, cultures obtained from patients with certain genetic diseases are available. A number of cells appear to be sensitive to some chemical or physical mutagens. These include cells from patients suffering from xeroderma pigmentosum, Ataxia telangiectasia, Fanconi's anemia, Cockayne's syndrome. However, only in the case of xeroderma pigmentosum cells, has the sensitivity to ultraviolet light been clearly correlated with a deficiency in excision repair of pyrimidine dimers. Furthermore the work with strains obtained from biopsies from man is difficult because these cells generally have low cloning efficiencies and also have a limited lifespan in vitro. It is therefore very important that more repair deficient mutants will become available from established cell lines from human or animal origin

  3. System turnover and acceptance procedure of Qingshan NPP

    Turnover and acceptance process of nuclear power plant has direct influence on construction scheduling. The authors describe organization, conditions and implementation of system turnover and acceptance. The paper also gives some suggestions as reference of turnover and acceptance work

  4. Wrinkled DNA.

    Arnott, S.; Chandrasekaran, R.; Puigjaner, L C; Walker, J K; Hall, I H; Birdsall, D L; Ratliff, R L

    1983-01-01

    The B form of poly d(GC):poly d(GC) in orthorhombic microcrystallites in oriented fibers has a secondary structure in which a dinucleotide is the repeated motif rather than a mononucleotide as in standard, smooth B DNA. One set of nucleotides (probably GpC) has the same conformations as the smooth form but the alternate (CpG) nucleotides have a different conformation at C3'-O3'. This leads to a distinctive change in the orientation of the phosphate groups. Similar perturbations can be detecte...

  5. Promoter Hypermethylation of DNA Repair Gene MGMT in Laryngeal Squamous Cell Carcinoma

    2006-01-01

    The relationship between hypermethylation of CpG islands in the promoter regions of O6methylguanine DNA methyltransferase (MGMT)genes and laryngeal squamous cell carcinoma was explored. Methylation-specific PCR and semi-quantitative RT-PCR were used to study the promoter methylation and mRNA expression of the MGMT gene in laryngeal carcinoma tissues, t issues adjacent to the tumor and normal laryngeal tissues. Hypermethylation of MGMT gene was detected in 16 samples of 46 (34.8 %) laryngeal squamous cell carcinoma samples. However, the MGMT hypermethylation was not detected in all tissues adjacent to the tumors and normal tissues. No significant difference in MGMT gene hypermethylation was found in samples with different histological grades (x2= 3. 130, P=0. 077) or in samples from patients with different TNM status (x2=3. 957, P=0. 138). No expression of MGMT mRNA was detected in all hypermethylated laryngeal carcinoma tissues. The expression of MGMT mRNA was detected in all unmethylated laryngeal carcinoma tissues, tissues adjacent to the tumors and normal tissues. It suggests that MGMT gene promoter hypermethylation is associated with MGMT gene transcription loss in laryngeal carcinoma tissues and possibly plays an important role in carcinogenesis of laryngeal tissues.

  6. DNA methylation profiling identifies two splenic marginal zone lymphoma subgroups with different clinical and genetic features.

    Arribas, Alberto J; Rinaldi, Andrea; Mensah, Afua A; Kwee, Ivo; Cascione, Luciano; Robles, Eloy F; Martinez-Climent, Jose A; Oscier, David; Arcaini, Luca; Baldini, Luca; Marasca, Roberto; Thieblemont, Catherine; Briere, Josette; Forconi, Francesco; Zamò, Alberto; Bonifacio, Massimiliano; Mollejo, Manuela; Facchetti, Fabio; Dirnhofer, Stephan; Ponzoni, Maurilio; Bhagat, Govind; Piris, Miguel A; Gaidano, Gianluca; Zucca, Emanuele; Rossi, Davide; Bertoni, Francesco

    2015-03-19

    Splenic marginal zone lymphoma is a rare lymphoma. Loss of 7q31 and somatic mutations affecting the NOTCH2 and KLF2 genes are the commonest genomic aberrations. Epigenetic changes can be pharmacologically reverted; therefore, identification of groups of patients with specific epigenomic alterations might have therapeutic relevance. Here we integrated genome-wide DNA-promoter methylation profiling with gene expression profiling, and clinical and biological variables. An unsupervised clustering analysis of a test series of 98 samples identified 2 clusters with different degrees of promoter methylation. The cluster comprising samples with higher-promoter methylation (High-M) had a poorer overall survival compared with the lower (Low-M) cluster. The prognostic relevance of the High-M phenotype was confirmed in an independent validation set of 36 patients. In the whole series, the High-M phenotype was associated with IGHV1-02 usage, mutations of NOTCH2 gene, 7q31-32 loss, and histologic transformation. In the High-M set, a number of tumor-suppressor genes were methylated and repressed. PRC2 subunit genes and several prosurvival lymphoma genes were unmethylated and overexpressed. A model based on the methylation of 3 genes (CACNB2, HTRA1, KLF4) identified a poorer-outcome patient subset. Exposure of splenic marginal zone lymphoma cell lines to a demethylating agent caused partial reversion of the High-M phenotype and inhibition of proliferation. PMID:25612624

  7. Sustainability and acceptance - new challenges for nuclear energy

    This paper discusses the concept of sustainability in relation to acceptance of nuclear energy. Acceptance is viewed in terms of public acceptance, industrial acceptance, and internal acceptance/consensus within the nuclear community. It addresses sustainability criteria, the need for innovation, and the different levels of acceptability. The mechanisms of risk perception are discussed along with the technological consequences from risk perception mechanisms leading to specific objections against nuclear energy. (author)

  8. Patients’ acceptance of corticotomy-assisted orthodontics

    Zawawi, Khalid H

    2015-01-01

    Objective To study patients’ acceptance of corticotomy-assisted orthodontics as a treatment option. Methods Adult patients seeking orthodontic treatment were asked to complete two sets of questionnaires; the first set included questions about age, sex, and level of education and general questions about orthodontic treatment; and the second set was related to the corticotomy-assisted orthodontics. Before answering the corticotomy questions, a brief description of the clinical procedure was explained and photographs of an actual procedure were shown. Results A total of 150 subjects were approached and 129 (86%) agreed to answer the questionnaires (72 male and 57 female patients). Of these, only 3.1% did hear about corticotomy and 7.8% selected corticotomy instead of extraction. Fear from the surgery (53.2%) was the most frequent reason for not selecting corticotomy followed by fear from pain (36.9%). The acceptance of corticotomy between males and females was similar. No relationship was found between the level of education and prior knowledge of the procedure, P=0.857. Prior knowledge about corticotomy was not a factor in selecting it as a treatment option (P=0.556) to reduce the treatment time (P=0.427). Conclusion The acceptance of corticotomy-assisted orthodontics as a treatment option was low. Fear from the surgery was the main reason for not selecting it. The acceptance of corticotomy-assisted orthodontics was not related to patient’s level of education or sex. PMID:26316719

  9. Treatment Acceptability among Mexican American Parents

    Borrego, Joaquin, Jr.; Ibanez, Elizabeth S.; Spendlove, Stuart J.; Pemberton, Joy R.

    2007-01-01

    There is a void in the literature with regard to Hispanic parents' views about common interventions for children with behavior problems. The purpose of this study was to examine the treatment acceptability of child management techniques in a Mexican American sample. Parents' acculturation was also examined to determine if it would account for…

  10. International Emissions Trading : Design and Political Acceptability

    Boom, Jan Tjeerd

    2006-01-01

    This thesis discusses the design and political acceptability of international emissions trading. It is shown that there are several designs options for emissions trading at the national level that have a different impact on output and thereby related factors such as employment and consumer prices. T

  11. Acceptance of Swedish e-health services

    Jung, Mary-Louise; Loria, Karla

    2010-01-01

    Objective: To investigate older people’s acceptance of e-health services, in order to identify determinants of, and barriers to, their intention to use e-health. Method: Based on one of the best-established models of technology acceptance, Technology Acceptance Model (TAM), in-depth exploratory interviews with twelve individuals over 45 years of age and of varying backgrounds are conducted. Results: This investigation could find support for the importance of usefulness and perceived ease of use of the e-health service offered as the main determinants of people’s intention to use the service. Additional factors critical to the acceptance of e-health are identified, such as the importance of the compatibility of the services with citizens’ needs and trust in the service provider. Most interviewees expressed positive attitudes towards using e-health and find these services useful, convenient, and easy to use. Conclusion: E-health services are perceived as a good complement to traditional health care service delivery, even among older people. These people, however, need to become aware of the e-health alternatives that are offered to them and the benefits they provide. PMID:21289860

  12. Acceptance test report: Backup power system

    Acceptance Test Report for construction functional testing of Project W-030 Backup Power System. Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks. Backup power includes a single 125 KW diesel generator, three 10-kva uninterruptible power supply units, and all necessary control

  13. Nevada National Security Site Waste Acceptance Criteria

    none,

    2013-06-01

    This document establishes the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Field Office (NNSA/NFO), Nevada National Security Site Waste Acceptance Criteria (NNSSWAC). The NNSSWAC provides the requirements, terms, and conditions under which the Nevada National Security Site (NNSS) will accept the following: • DOE hazardous and non-hazardous non-radioactive classified waste • DOE low-level radioactive waste (LLW) • DOE mixed low-level waste (MLLW) • U.S. Department of Defense (DOD) classified waste The LLW and MLLW listed above may also be classified waste. Classified waste is the only waste accepted for disposal that may be non-radioactive and shall be required to meet the waste acceptance criteria for radioactive waste as specified in this document. Classified waste may be sent to the NNSS as classified matter. Section 3.1.18 provides the requirements that must be met for permanent burial of classified matter. The NNSA/NFO and support contractors are available to assist the generator in understanding or interpreting this document. For assistance, please call the NNSA/NFO Environmental Management Operations (EMO) at (702) 295-7063, and the call will be directed to the appropriate contact.

  14. Nevada National Security Site Waste Acceptance Criteria

    This document establishes the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office (NNSA/NSO), Nevada National Security Site Waste Acceptance Criteria (NNSSWAC). The NNSSWAC provides the requirements, terms, and conditions under which the Nevada National Security Site (NNSS) will accept DOE non-radioactive classified waste, DOE non-radioactive hazardous classified waste, DOE low-level radioactive waste (LLW), DOE mixed low-level waste (MLLW), and U.S. Department of Defense (DOD) classified waste for permanent disposal. Classified waste is the only waste accepted for disposal that may be non-radioactive and will be required to meet the waste acceptance criteria for radioactive waste as specified in this document. The NNSA/NSO and support contractors are available to assist you in understanding or interpreting this document. For assistance, please call the NNSA/NSO Waste Management Project (WMP) at (702) 295-7063, and your call will be directed to the appropriate contact.

  15. Nevada National Security Site Waste Acceptance Criteria

    This document establishes the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Field Office (NNSA/NFO), Nevada National Security Site Waste Acceptance Criteria (NNSSWAC). The NNSSWAC provides the requirements, terms, and conditions under which the Nevada National Security Site (NNSS) will accept the following: DOE hazardous and non-hazardous non-radioactive classified waste; DOE low-level radioactive waste (LLW); DOE mixed low-level waste (MLLW); and, U.S. Department of Defense (DOD) classified waste. The LLW and MLLW listed above may also be classified waste. Classified waste is the only waste accepted for disposal that may be non-radioactive and shall be required to meet the waste acceptance criteria for radioactive waste as specified in this document. Classified waste may be sent to the NNSS as classified matter. Section 3.1.18 provides the requirements that must be met for permanent burial of classified matter. The NNSA/NFO and support contractors are available to assist the generator in understanding or interpreting this document. For assistance, please call the NNSA/NFO Environmental Management Operations (EMO) at (702) 295-7063, and the call will be directed to the appropriate contact.

  16. Peer Acceptance and Friendship: An Investigation.

    Bishop, Julia A.; Inderbitzen, Heidi M.

    1995-01-01

    Investigated how peer acceptance and friendship were related to self-esteem in adolescents. Ninth-grade students (n=542) were classified by sociometric group and presence or absence of reciprocal friendships. Results indicate no significant difference in self-esteem scores across sociometric groups. Subjects with one friend had higher self-esteem…

  17. Generator acceptance test and inspection report

    This Acceptance Test Report(ATR) is the completed testing and inspection of the new portable generator. The testing and inspection is to verify that the generator provided by the vendor meets the requirements of specification WHC-S-0252, Revision 2. Attached is various other documentation to support the inspection and testing

  18. Are we ready to accept the challenge?

    Lau, Sofie Rosenlund; Traulsen, Janine M

    2016-01-01

    administrative pharmacy, this study challenges contributors to the field by asking: Are we ready to accept the challenge and take qualitative research one step further? PURPOSE: The purpose of this study was to initiate a constructive dialogue on the need for increased transparency in qualitative data analysis...

  19. Is Coersive Treatment of Offenders Morally Acceptable?

    Ryberg, Jesper

    2015-01-01

    Is it morally acceptable to instigate criminal offenders to participate in rehabilitative treatment by offering treatment in return for early release from prison? Some theorists have supported such treatment schemes by pointing to the beneficial consequences that follow from the treatment. Others...

  20. Void fraction instrument acceptance test procedure

    This acceptance test procedure (ATP) was written to test the void fraction instrument (VFI) and verify that the unit is ready for field service. The procedure verifies that the mechanical and electrical features (not specifically addressed in the software ATP) and software alarms are operating as designed

  1. Acceptance of homosexuality in the Netherlands 2011

    Saskia Keuzenkamp

    2011-01-01

    The Dutch government wishes to promote the social acceptance of homosexuality. To gain an impression of the current status and the progress in achieving this objective, the government asked the Netherlands Institute for Social Research/SCP to carry out a study of the current statistics and trends in

  2. Monitoring Acceptance of homosexuality in the Netherlands

    Saskia Keuzenkamp

    2010-01-01

    Promoting acceptance of homosexuality is the main objective of current Dutch policy on the emancipation of gays and lesbians. At the request of Ronald Plasterk, the former Dutch government minister with responsibility for this policy, the Netherlands Institute for Social Research/SCP is monitoring d

  3. Influence Processes for Information Technology Acceptance

    Bhattacherjee, Anol; Sanford, Clive Carlton

    2006-01-01

    This study examines how processes of external influence shape information technology acceptance among potential users, how such influence effects vary across a user population, and whether these effects are persistent over time. Drawing on the elaboration-likelihood model (ELM), we compared two...

  4. W-087 Acceptance test procedure. Revision 1

    Joshi, A.W.

    1997-06-10

    This Acceptance Test Procedure/Operational Test Procedure (ATP/OTP) has been prepared to demonstrate that the Electrical/Instrumentation and Mechanical systems function as required by project criteria and to verify proper operation of the integrated system including the interlocks.

  5. Nevada National Security Site Waste Acceptance Criteria

    NSTec Environmental Management

    2012-02-28

    This document establishes the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office (NNSA/NSO), Nevada National Security Site Waste Acceptance Criteria (NNSSWAC). The NNSSWAC provides the requirements, terms, and conditions under which the Nevada National Security Site (NNSS) will accept DOE non-radioactive classified waste, DOE non-radioactive hazardous classified waste, DOE low-level radioactive waste (LLW), DOE mixed low-level waste (MLLW), and U.S. Department of Defense (DOD) classified waste for permanent disposal. Classified waste is the only waste accepted for disposal that may be non-radioactive and will be required to meet the waste acceptance criteria for radioactive waste as specified in this document. The NNSA/NSO and support contractors are available to assist you in understanding or interpreting this document. For assistance, please call the NNSA/NSO Waste Management Project (WMP) at (702) 295-7063, and your call will be directed to the appropriate contact.

  6. Acceptance of nuclear power: The Fukushima effect

    Utilizing a longitudinal study design, the impact of the 2011 accident in Fukushima on acceptance of nuclear power and the evaluation of several scenarios with different percentages of nuclear power were examined. Mail surveys were conducted in the German-speaking part of Switzerland. The first survey took place before the accident in Fukushima (Autumn 2010), the second survey immediately after the accident (March 2011), and the third survey half a year after the accident (October 2011). A sample of 463 persons participated in all three surveys. The accident had a negative impact on the acceptance of nuclear power. The mean change was moderate, and high correlations between the measurement points were observed. Overall, participants thus showed rather stable attitudes towards nuclear power across the three measurement waves. Results of the present study demonstrate the importance of prior beliefs and attitudes for the interpretation of an accident. The evaluation of the various scenarios was strongly influenced by participants’ pre-Fukushima attitudes towards nuclear power. - Highlights: ► Longitudinal studies are important for risk perception research. ► The accident in Fukushima had only a moderate impact on acceptance. ► Acceptance of nuclear power before and after Fukushima was highly correlated. ► People have stable attitudes towards nuclear power

  7. 29 CFR 1926.29 - Acceptable certifications.

    2010-07-01

    ... Acceptable certifications. (a) Pressure vessels. Current and valid certification by an insurance company or... be in compliance with the requirements of this part when evidence of current and valid certification by an insurance company or regulatory authority attesting to the safe installation, inspection,...

  8. Acceptance of irradiated food: an education issue

    The commercial use of irradiated food technology in Brazil has a slow growing due to misinterpretation by most Brazilian consumers, who have been mislead by wrong ideas about the meaning of what is nuclear energy. Researches indicate that consumers have difficult in accepting such a technology due to the confusion between the terms irradiation and radioactivity, which are often related to health risks. When properly informed about the process, its purpose and the benefits offered by food irradiation technology, most consumers react positively. Therefore, this work aims to: first, to evaluate the acceptance of irradiated foods by Brazilian consumers; second, to verify the teaching at school about the food irradiation process; third, to analyze the Brazilian school curriculum from elementary school to high school, regarding nuclear energy applications; then, to compare the content taught in Brazil with the content covered in other surveyed countries, such as France, United States, and China. The methodology of this study consisted of a systematic survey of the specific literature, and a questionnaire to verify the acceptance of irradiated food by Brazilian consumers. According to the researched bibliography, it was clear the recommendation of an early school education about the usage of nuclear energy, more specifically, food irradiation. Such a recommendation is due to the fact that the consulted costumers, in Brazil and other countries mentioned in this work, do not clearly understand the full benefits of irradiated food. Hence, education is fundamental for the acceptance of new technologies by consumers, as it is the case with irradiated food. (author)

  9. INS Director SHI Xianglin's resignation accepted

    2007-01-01

    @@ The CAS Shanghai Institutes for Biological Sciences (SIBS) has accepted the resignation of Dr. SHI Xianglin as director of the Institute for Nutritional Sciences (INS), and decided that Dr. CHEN Yan, deputy director of INS, would take the helm of the institute for the time being.

  10. Accepting the truth: A step toward reconciliation

    Ilić Zoran

    2002-01-01

    Full Text Available The truth is not only about accepting the facts. The truth includes emotional components - longing for acknowledgment of mistakes and validation of painful losses and experiences. While one side in the conflict considers itself as the only victim and experiences determination and acknowledgment of the truth as a possibility for healing its own trauma through satisfaction of justice and compensation, the other side is not accepting the truth. For this other side, confrontation with the facts is a painful trauma, which endangers individual moral norms, threatens the national identity and requires, on individual level, plugging in the defense mechanism, in order to prevent penetration of painful emotions into the consciousness. Primitive psychological mechanisms of defense, focused around splitting (projection, denial, are the main obstacles in accepting the truth both on individual and group level. There is also another extreme form of reaction manifested through "hypertrophy" of the truth and one-sided self-accusation. In this paper the author presents psychological explanation of the process of accepting the truth as the prerequisite for reconciliation and transformation of the conflict.

  11. Workaholism, Health, and Self-Acceptance

    Chamberlin, Christine M.; Zhang, Naijian

    2009-01-01

    This study examined the relationships between workaholism, perceived parental workaholism, self-acceptance, psychological well-being, and physical symptoms among 347 college students. Statistically significant relationships were found between college students' perceived parental workaholism and their own workaholism. Also, relationships between…

  12. Measuring acceptance of new food product concepts

    Grunert, Klaus G; Perrea, Toula; Kügler, Jens

    and convenience, or without cognitive mediation (see figure). Using examples of new pork-based product concepts and data from four countries (Denmark, Germany, Netherlands, UK), we elaborate on the role of these two routes towards new product acceptance. We propose that the increased role of storytelling...

  13. Women's role for public acceptance - WEN activities

    WEN (Women's Energy Network) is a national network of women working professionally in the field of public relations or technologies in various energy-related companies and organizations or interested in energy public acceptance (PA) activities including nuclear PA. This paper describes goals, organization, activities of WEN, and survey on public consciousness. 10 figs

  14. φ29 DNA polymerase

    Blanco, Luis; Bernad, Antonio; Salas, Margarita

    1996-01-01

    An improved method for determining the nucleotide base sequence of a DNA molecule employs a φ-29 type DNA polymerase modified to have reduced or no exonuclease activity. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleo...

  15. Patients’ acceptance of corticotomy-assisted orthodontics

    Zawawi KH

    2015-08-01

    Full Text Available Khalid H Zawawi Department of Orthodontics, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia Objective: To study patients’ acceptance of corticotomy-assisted orthodontics as a treatment option.Methods: Adult patients seeking orthodontic treatment were asked to complete two sets of questionnaires; the first set included questions about age, sex, and level of education and general questions about orthodontic treatment; and the second set was related to the corticotomy-assisted orthodontics. Before answering the corticotomy questions, a brief description of the clinical procedure was explained and photographs of an actual procedure were shown.Results: A total of 150 subjects were approached and 129 (86% agreed to answer the questionnaires (72 male and 57 female patients. Of these, only 3.1% did hear about corticotomy and 7.8% selected corticotomy instead of extraction. Fear from the surgery (53.2% was the most frequent reason for not selecting corticotomy followed by fear from pain (36.9%. The acceptance of corticotomy between males and females was similar. No relationship was found between the level of education and prior knowledge of the procedure, P=0.857. Prior knowledge about corticotomy was not a factor in selecting it as a treatment option (P=0.556 to reduce the treatment time (P=0.427.Conclusion: The acceptance of corticotomy-assisted orthodontics as a treatment option was low. Fear from the surgery was the main reason for not selecting it. The acceptance of corticotomy-assisted orthodontics was not related to patient’s level of education or sex. Keywords: orthodontic treatment, corticotomy-assisted orthodontics, patient acceptance, cross sectional survey, alveolar decortication

  16. Optimised electroporation mediated DNA vaccination for treatment of prostate cancer.

    Ahmad, Sarfraz

    2010-01-01

    ABSTRACT: BACKGROUND: Immunological therapies enhance the ability of the immune system to recognise and destroy cancer cells via selective killing mechanisms. DNA vaccines have potential to activate the immune system against specific antigens, with accompanying potent immunological adjuvant effects from unmethylated CpG motifs as on prokaryotic DNA. We investigated an electroporation driven plasmid DNA vaccination strategy in animal models for treatment of prostate cancer. METHODS: Plasmid expressing human PSA gene (phPSA) was delivered in vivo by intra-muscular electroporation, to induce effective anti-tumour immune responses against prostate antigen expressing tumours. Groups of male C57 BL\\/6 mice received intra-muscular injections of phPSA plasmid. For phPSA delivery, quadriceps muscle was injected with 50 mug plasmid. After 80 seconds, square-wave pulses were administered in sequence using a custom designed pulse generator and acustom-designed applicator with 2 needles placed through the skin central to the muscle. To determine an optimum treatment regimen, three different vaccination schedules were investigated. In a separate experiment, the immune potential of the phPSA vaccine was further enhanced with co- administration of synthetic CpG rich oligonucleotides. One week after last vaccination, the mice were challenged subcutaneously with TRAMPC1\\/hPSA (prostate cancer cell line stably expressing human PSA) and tumour growth was monitored. Serum from animals was examined by ELISA for anti-hPSA antibodies and for IFNgamma. Histological assessment of the tumours was also carried out. In vivo and in vitro cytotoxicity assays were performed with splenocytes from treated mice. RESULTS: The phPSA vaccine therapy significantly delayed the appearance of tumours and resulted in prolonged survival of the animals. Four-dose vaccination regimen provided optimal immunological effects. Co - administration of the synthetic CpG with phPSA increased anti-tumour responses

  17. The multivariate association between genomewide DNA methylation and climate across the range of Arabidopsis thaliana.

    Keller, Thomas E; Lasky, Jesse R; Yi, Soojin V

    2016-04-01

    Epigenetic changes can occur due to extracellular environmental conditions. Consequently, epigenetic mechanisms can play an intermediate role to translate environmental signals to intracellular changes. Such a role might be particularly important in plants, which often show strong local adaptation and have the potential for heritable epigenetic states. However, little is currently known about the role of epigenetic variation in the ecological mechanisms of adaptation. Here, we used multivariate redundancy analyses to examine genomewide associations between DNA methylation polymorphisms and climate variation in two independent panels of Arabidopsis accessions, including 122 Eurasian accessions as well as in a regional panel of 148 accessions in Sweden. At the single-nucleotide methylation level, climate and space (geographic spatial structure) explain small yet significant amount of variation in both panels. On the other hand, when viewed in a context of genomic clusters of methylated and unmethylated cytosines, climate and space variables explain much greater amounts of variation in DNA methylation than those explained by variation at the single-nucleotide level. We found that the single-nucleotide methylation polymorphisms with the strongest associations with climate were enriched in transposable elements and in potentially RNA-directed methylation contexts. When viewed in the context of genomic clusters, variation of DNA methylation at different sequence contexts exhibit distinctive segregation along different axes of variation in the redundancy analyses. Genomewide methylation showed much stronger associations with climate within the regional panel (Sweden) compared to the global (Eurasia). Together, these findings indicate that genetic and epigenetic variation across the genome may play a role in response to climate conditions and local adaptation. PMID:26850505

  18. TRX-LOGOS - a graphical tool to demonstrate DNA information content dependent upon backbone dynamics in addition to base sequence

    Fortin, Connor H.; Schulze, Katharina V.; Babbitt, Gregory A.

    2015-01-01

    Background It is now widely-accepted that DNA sequences defining DNA-protein interactions functionally depend upon local biophysical features of DNA backbone that are important in defining sites of binding interaction in the genome (e.g. DNA shape, charge and intrinsic dynamics). However, these physical features of DNA polymer are not directly apparent when analyzing and viewing Shannon information content calculated at single nucleobases in a traditional sequence logo plot. Thus, sequence lo...

  19. DNA ligase I, the replicative DNA ligase

    Howes, Timothy R.L.; Tomkinson, Alan E.

    2012-01-01

    Multiple DNA ligation events are required to join the Okazaki fragments generated during lagging strand DNA synthesis. In eukaryotes, this is primarily carried out by members of the DNA ligase I family. The C-terminal catalytic region of these enzymes is composed of three domains: a DNA binding domain, an adenylation domain and an OB-fold domain. In the absence of DNA, these domains adopt an extended structure but transition into a compact ring structure when they engage a DNA nick, with each...

  20. Synthesis of DNA oligonucleotides containing C5-ethynylbenzenesulfonamide-modified nucleotides (EBNA) by polymerases towards the construction of base functionalized nucleic acids.

    Goubet, Astrid; Chardon, Antoine; Kumar, Pawan; Sharma, Pawan K; Veedu, Rakesh N

    2013-02-01

    C5-Ethynylbenzenesulfonamide-modified nucleotide (EBNA) was investigated as substrate of various DNA polymerases. The experiments revealed that KOD, Phusion and Klenow DNA polymerases successfully accepted EBNA-T nucleotide as a substrate and yielded the fully extended DNA. KOD DNA polymerase was found to be the most efficient enzyme to furnish EBNA-T containing DNA in good yields. Phusion DNA polymerase efficiently amplified the template containing EBNA-T nucleotides by PCR. PMID:23265899

  1. Strategies for Fostering HPV Vaccine Acceptance

    2006-01-01

    Full Text Available Vaccines that protect against infection with the types of human papillomavirus (HPV commonly associated with cervical cancer (HPV 16 and 18 and genital warts (HPV 6 and 11 are expected to become available in the near future. Because HPV vaccines are prophylactic, they must be administered prior to exposure to the virus, ideally during preadolescence or adolescence. The young age of the target vaccination population means that physicians, parents, and patients will all be involved in the decision-making process. Research has shown that parents and patients are more likely to accept a vaccine if it is efficacious, safe, reasonably priced, and recommended by a physician. Widespread education of physicians, patients, and parents about the risks and consequences of HPV infection and the benefits of vaccination will be instrumental for fostering vaccine acceptance.

  2. GERDA phase II detectors and acceptance tests

    The GERDA collaboration aims at searching for the neutrinoless double beta decay of 76Ge into 76Se. It uses enriched Ge detectors that are operated in liquid argon. Phase I of the experiment started with a target mass of approximately 20 kg on November 1, 2011, and it will last for 1 year. Phase II is in preparation and envisions the installation of at least 25 new Broad Energy Ge detectors (BEGe) that will increase the target mass by nearly 20 kg. These detectors, however, have to be extensively tested prior to their usage. The talk briefly reviews the preparation of the detector screening campaign and focuses on the corresponding acceptance test strategy. The tests include energy resolution and leakage current measurements, dead layer and active volume determination, as well as the pulse shape discrimination efficiencies of the detectors. Finally we present results obtained with depleted BEGe detectors that are used for the optimization of the enriched ones during the acceptance tests.

  3. Nevada test site waste acceptance criteria

    This document provides the requirements, terms, and conditions under which the Nevada Test Site (NTS) will accept low-level radioactive and mixed waste for disposal; and transuranic and transuranic mixed waste for interim storage at the NTS. Review each section of this document. This document is not intended to include all of the requirements; rather, it is meant as a guide toward meeting the regulations. All references in this document should be observed to avoid omission of requirements on which acceptance or rejection of waste will be based. The Department of Energy/Nevada Operations Office (DOE/NV) and support contractors are available to assist you in understanding or interpreting this document

  4. Acceptable risk in reactor safety studies

    Acceptable risk is defined in terms of its five basic parameters: the hazard or problem; the probability of occurrence; the consequence; the possible alternative actions; and the value system of the community or the society. The problem of consistency in design at a site and between differing sites is discussed and solutions are suggested. Techniques for consistent deterministic and probabilistic setting limits and design standards are illustrated using data from AEC Reactor Safety Study, WASH-1400. The influence of level of consequence is discussed and a general methodology for decision analysis in resource allocation problem is briefly introduced and illustrated. The concept of acceptable risk is put in a quantitative format that can be used by engineers and planners. Bayesian statistical methods are introduced to develop the methodologies

  5. Radioactive waste disposal and public acceptance aspects

    Part of the public opinion around the world considers the wastes generated due to nuclear applications as the biggest environmental problem of the present time. The development of a solution that satisfies everybody is a great challenge, in that obtaining public acceptance for nuclear enterprises is much more challenging than solving the technical issues involved. Considering that the offering of a final solution that closes the radioactive waste cycle has a potentially positive impact on public opinion, the objective of this work is to evaluate the amount of the radioactive waste volume disposed in a five-year period in several countries and gauge the public opinion regarding nuclear energy. The results show that the volume of disposed radioactive waste increased, a fact that stresses the importance of promoting discussions about repositories and public acceptance. (author)

  6. Design of Large Momentum Acceptance Transport Systems

    Douglas, David

    2005-01-01

    The use of energy recovery to enable high power linac operation often gives rise to an attendant challenge - the transport of high power beams subtending large phase space volumes. In particular applications - such as FEL driver accelerators - this manifests itself as a requirement for beam transport systems with large momentum acceptance. We will discuss the design, implementation, and operation of such systems. Though at times counterintuitive in behavior (perturbative descriptions may, for example, be misleading), large acceptance systems have been successfully utilized for generations as spectrometers and accelerator recirculators.* Such systems are in fact often readily designed using appropriate geometric descriptions of beam behavior; insight provided using such a perspective may in addition reveal inherent symmetries that simplify construction and improve operability. Our discussion will focus on two examples: the Bates-clone recirculator used in the Jefferson Lab 10 kW IR Upgrade FEL (which has an ob...

  7. Void fraction instrument acceptance test procedure

    This document presents the results of the acceptance test for the mechanical and electrical features (not specifically addressed by the software ATP) of the void fraction instrument (VFI). Acceptance testing of the VFI, control console, and decontamination spray assembly was conducted in the 306E building high bay and area adjacent to the facility. The VFI was tested in the horizontal position supported in multiple locations on rolling tables. The control console was located next to the VFI pneumatic control assembly. The VFI system was operated exactly as is expected in the tank farm, with the following exceptions: power was provided from a building outlet and the VFI was horizontal. The testing described in this document verifies that the mechanical and electrical features are operating as designed and that the unit is ready for field service

  8. Radioactive waste disposal and public acceptance aspects

    Ulhoa, Barbara M.A.; Aleixo, Bruna L.; Mourao, Rogerio P.; Ferreira, Vinicius V.M., E-mail: mouraor@cdtn.b, E-mail: vvmf@cdtn.b [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil)

    2011-07-01

    Part of the public opinion around the world considers the wastes generated due to nuclear applications as the biggest environmental problem of the present time. The development of a solution that satisfies everybody is a great challenge, in that obtaining public acceptance for nuclear enterprises is much more challenging than solving the technical issues involved. Considering that the offering of a final solution that closes the radioactive waste cycle has a potentially positive impact on public opinion, the objective of this work is to evaluate the amount of the radioactive waste volume disposed in a five-year period in several countries and gauge the public opinion regarding nuclear energy. The results show that the volume of disposed radioactive waste increased, a fact that stresses the importance of promoting discussions about repositories and public acceptance. (author)

  9. European consumers' acceptance of beef processing technologies

    de Barcellos, Marcia Dutra; Kügler, Jens Oliver; Grunert, Klaus G.;

    2010-01-01

    The use of new technologies in beef production chains may affect consumers' opinion of meat products. A qualitative study was performed to investigate consumers' acceptance of seven beef processing technologies: marinating by injection aiming for increased 1) healthiness; 2) safety; and 3) eating...... quality; 4) marinating by submerging aiming for increased eating quality; 5) nutritional enhancement and restructuring through enzyme binding; 6) shock wave treatment and 7) thermal processing. Participants' attitudes towards beef, their innovativeness and risk aversion were also assessed. In total, 65...... adults (19-60 years old) participated in eight focus groups in Spain, France, Germany and the UK. Results suggested a relationship between acceptance of new beef products, technology familiarity and perceived risks related to its application. Excessive manipulation and fear of moving away from 'natural...

  10. Transport efficiency in large acceptance spectrometers

    Cavallaro, M., E-mail: manuela.cavallaro@lns.infn.i [INFN-Laboratori Nazionali del Sud, Via S. Sofia 62, I-95125 Catania (Italy); Cappuzzello, F.; Carbone, D.; Cunsolo, A. [INFN-Laboratori Nazionali del Sud, Via S. Sofia 62, I-95125 Catania (Italy); Dipartimento di Fisica e Astronomia, Universita di Catania, Via S. Sofia 64, I-95125 Catania (Italy); Foti, A. [Dipartimento di Fisica e Astronomia, Universita di Catania, Via S. Sofia 64, I-95125 Catania (Italy); INFN-Sezione di Catania, Via S. Sofia 64, I-95125 Catania (Italy); Linares, R. [Instituto de Fisica, Universidade Federal Fluminense, Av. Litoranea s/n, Gragoata, Niteroi, RJ 24210-340 (Brazil)

    2011-05-01

    A general technique to study the transmission efficiency of charged particles through a large acceptance magnetic device is presented. This basically involves the measurement of the impact positions and angles of the particles in any plane normal to the optical axis and the use of a powerful algorithm for the reconstruction of the trajectories. This latter is designed to perform the calculations of highly non-linear transport functions and is essential when high-order aberrations are not negligible. The technique is applied to study the transport efficiency of the heavy ions produced in different nuclear reactions and analyzed by the MAGNEX large acceptance spectrometer. Both the angular integral and differential efficiency have been deduced with overall accuracy of about {+-}1.5% and {+-}5%, respectively.

  11. Nevada National Security Site Waste Acceptance Criteria

    NSTec Environmental Management

    2011-01-01

    This document establishes the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office (NNSA/NSO) Nevada National Security Site Waste Acceptance Criteria (NNSSWAC). The NNSSWAC provides the requirements, terms, and conditions under which the Nevada National Security Site (NNSS) will accept low-level radioactive waste and mixed low-level waste for disposal. The NNSSWAC includes requirements for the generator waste certification program, characterization, traceability, waste form, packaging, and transfer. The criteria apply to radioactive waste received at the NNSS Area 3 and Area 5 Radioactive Waste Management Complex for disposal. The NNSA/NSO and support contractors are available to assist you in understanding or interpreting this document. For assistance, please call the NNSA/NSO Waste Management Project at (702) 295-7063 or fax to (702) 295-1153.

  12. Nevada National Security Site Waste Acceptance Criteria

    NSTec Environmental Management

    2010-09-03

    This document establishes the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office (NNSA/NSO) Nevada National Security Site Waste Acceptance Criteria (NNSSWAC). The NNSSWAC provides the requirements, terms, and conditions under which the Nevada National Security Site (NNSS) will accept low-level radioactive waste and mixed low-level waste for disposal. The NNSSWAC includes requirements for the generator waste certification program, characterization, traceability, waste form, packaging, and transfer. The criteria apply to radioactive waste received at the NNSS Area 3 and Area 5 Radioactive Waste Management Complex for disposal. The NNSA/NSO and support contractors are available to assist you in understanding or interpreting this document. For assistance, please call the NNSA/NSO Waste Management Project at (702) 295-7063 or fax to (702) 295-1153.

  13. Validation and acceptance of synthetic infrared imagery

    Smith, Moira I.; Bernhardt, Mark; Angell, Christopher R.; Hickman, Duncan; Whitehead, Philip; Patel, Dilip

    2004-08-01

    This paper describes the use of an image query database (IQ-DB) tool as a means of implementing a validation strategy for synthetic long-wave infrared images of sea clutter. Specifically it was required to determine the validity of the synthetic imagery for use in developing and testing automatic target detection algorithms. The strategy adopted for exploiting synthetic imagery is outlined and the key issues of validation and acceptance are discussed in detail. A wide range of image metrics has been developed to achieve pre-defined validation criteria. A number of these metrics, which include post processing algorithms, are presented. Furthermore, the IQ-DB provides a robust mechanism for configuration management and control of the large volume of data used. The implementation of the IQ-DB is reviewed in terms of its cardinal point specification and its central role in synthetic imagery validation and EOSS progressive acceptance.

  14. TOFD and acceptance criteria: a perfect team

    In recent years, it has become clear that the Time Of Flight Diffraction (TOFD) technique, developed in the UK in the seventies, is a most powerful technique not only for accurate sizing of known defects but also for defect detection in routine NDT. But, whereas TOFD may have been successfully introduced for recurrent inspections and fingerprinting, using Engineering Critical Assessment (ECA) or Fitness For Purpose (FFP) approaches, the lack of adequate acceptance criteria still prohibits its introduction for routine inspections. This paper also addresses a Dutch project that aims for the development of defect acceptance criteria for TOFD inspection. Examples of TOFD applications are given, ranging from pipeline girth welds to vessels with wall thicknesses up to 400 mm. Some experiences on complex geometries as well as high-alloy steels and titanium will also be highlighted

  15. Nevada National Security Site Waste Acceptance Criteria

    This document establishes the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office (NNSA/NSO) Nevada National Security Site Waste Acceptance Criteria (NNSSWAC). The NNSSWAC provides the requirements, terms, and conditions under which the Nevada National Security Site (NNSS) will accept low-level radioactive waste and mixed low-level waste for disposal. The NNSSWAC includes requirements for the generator waste certification program, characterization, traceability, waste form, packaging, and transfer. The criteria apply to radioactive waste received at the NNSS Area 3 and Area 5 Radioactive Waste Management Complex for disposal. The NNSA/NSO and support contractors are available to assist you in understanding or interpreting this document. For assistance, please call the NNSA/NSO Waste Management Project at (702) 295-7063 or fax to (702) 295-1153.

  16. Patients’ acceptance of corticotomy-assisted orthodontics

    Zawawi KH

    2015-01-01

    Khalid H Zawawi Department of Orthodontics, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia Objective: To study patients’ acceptance of corticotomy-assisted orthodontics as a treatment option.Methods: Adult patients seeking orthodontic treatment were asked to complete two sets of questionnaires; the first set included questions about age, sex, and level of education and general questions about orthodontic treatment; and the second set was related to the corticoto...

  17. Patients’ acceptance of corticotomy-assisted orthodontics

    Zawawi, Khalid H

    2015-01-01

    Objective To study patients’ acceptance of corticotomy-assisted orthodontics as a treatment option. Methods Adult patients seeking orthodontic treatment were asked to complete two sets of questionnaires; the first set included questions about age, sex, and level of education and general questions about orthodontic treatment; and the second set was related to the corticotomy-assisted orthodontics. Before answering the corticotomy questions, a brief description of the clinical procedure was exp...

  18. Breathing air trailer acceptance test report

    This Acceptance Test Report documents compliance with the requirements of specification WHC-S-0251, Rev.0 and ECNs 613530 and 606113. The equipment was tested according to WHC-SD-WM-ATP-104. The equipment tested is a Breathing Air Supply Trailer purchased as a design and fabrication procurement activity. The ATP was written by the Seller and was performed by the Seller with representatives of the Westinghouse Hanford Company witnessing portions of the test at the Seller's location

  19. Acceptance of a Theory: Justification or Rhetoric?

    Chow, Siu L.

    1992-01-01

    The rhetoric-analytic critique of experimental psychology owes its apparent attractiveness to (a) some erroneous ideas about cognitive psychology and the rationale of experimentation, (b) the failure to distinguish between prior data and evidential data vis-à-vis the to-be-corroborated explanatory theory, and (c) evidential data owes their identity to a theory that is independent of the theory being tested. Theories in cognitive psychology are accepted because they can withstand concerted eff...

  20. Peer acceptance/rejection and academic achievement

    Spasenović Vera Z.

    2003-01-01

    Considerations of the nature and role of peer relations in child development indicate that peer interaction is an important factor in developing social and cognitive competences. Peer relations not only influence current but also subsequent academic, behavioral and emotional development. Accepted students more often display better academic achievement, whereas the status of rejection is coupled with academic difficulties and lower academic achievement. Peer rejection is a relatively stable ch...

  1. Sexual Education In Malaysia: Accepted Or Rejected?

    Siti Syairah Mohd Mutalip; Ruzianisra Mohamed

    2012-01-01

    Background: Introduction to sexual education in schools was suggested by the Malaysian government as one of the effort taken in the aim to reduce the sexual-related social problems among Malaysian teenagers nowadays. This study was proposed in the aim to determine the rate of acceptance among adolescents on the implementation of sexual education in schools. Methods: This study was conducted using questionnaires distributed to 152 pre-degree students in Faculty of Pharmacy, Universiti Teknolog...

  2. Assesing the acceptance of the project

    Clara Fernández-Shaw Toda; David García Marco; Inmaculada Díaz Fernández

    2009-01-01

    OBJECTIVE: Evaluate the acceptability of the project "Adding colour to your unit dose." MATERIAL AND METHODS: It sets in motion a project to reduce errors in the administration of pasting labels with different shapes and colours on blister medicines. It includes the name of the product, the active ingredient, dosage, lot and expiration date. Also included for internal quality control are the initials of the person who made the label and the date and time at which it was made. The nurse monito...

  3. Consumer Acceptance Of Functional Foods In Montenegro

    Zaklina Stojanovic; Jelena Filipovic; Boban Mugosa

    2013-01-01

    This study aimed to investigate determinants of acceptance of functional food in Montenegro and to analyze to what extent these factors affected individuals' consumption of food with health claims. The questionnaire was administered to 479 respondents in Montenegro. The stratified three-stage random sampling method was adopted. Several statistical techniques were employed for investigating data: regression, cluster analysis, independent-samples t test and chi-square. The results indicate that...

  4. Diesel generator trailer acceptance test procedure

    This Acceptance Test Procedure (ATP) will document compliance with the requirements of WHC-S-0252 Rev. 1 and ECNs 609271, and 609272. The equipment being tested is a 150KW Diesel Generator mounted on a trailer with switchgear. The unit was purchased as a Design and Fabrication procurement activity. The ATP was written by the Seller and will be performed by the Seller with representatives of the Westinghouse Hanford Company witnessing the test at the Seller's location

  5. Finite Automata with Generalized Acceptance Criteria

    Timo Peichl; Heribert Vollmer

    2001-01-01

    We examine the power of nondeterministic finite automata with acceptance of an input word defined by a leaf language, i.e., a condition on the sequence of leaves in the automaton's computation tree. We study leaf languages either taken from one of the classes of the Chomsky hierarchy, or taken from a time- or space-bounded complexity class. We contrast the obtained results with those known for leaf languages for Turing machines and Boolean circuits.

  6. Measuring social risk and determining its acceptability

    Lathrop, J.W.

    1978-07-14

    The implementation of a nuclear waste management technology raises several issues concerning the regulation of social risk. This paper presents a decision analytic approach to resolving some of those issues. A methodology for developing a radiological risk measure is presented, and several approaches to defining acceptable levels of that risk measure are considered. The methodology presented is oriented toward the development of radiological performance objectives for use as guidance in the drafting of regulations.

  7. Acceptance of homosexuality in the Netherlands 2011

    Saskia Keuzenkamp

    2011-01-01

    The Dutch government wishes to promote the social acceptance of homosexuality. To gain an impression of the current status and the progress in achieving this objective, the government asked the Netherlands Institute for Social Research/SCP to carry out a study of the current statistics and trends in this regard. This report shows that the Netherlands is still the most gay-tolerant country in Europe. Nonetheless, there are limits to that tolerance and there are some groups in Dutch society whi...

  8. Monitoring Acceptance of homosexuality in the Netherlands

    Saskia Keuzenkamp

    2010-01-01

    Promoting acceptance of homosexuality is the main objective of current Dutch policy on the emancipation of gays and lesbians. At the request of Ronald Plasterk, the former Dutch government minister with responsibility for this policy, the Netherlands Institute for Social Research/SCP is monitoring developments in society in this regard. This preliminary publication, which precedes a report to be published in June 2010, contains data from population surveys. What are the attitudes of the Dutch...

  9. Introduction to Technologies Acceptance and Sustainability Modelling

    Barkane, Zane; Ginters, Egils

    2011-01-01

    International audience Elaboration and introduction of a new technology is a complex and expensive task, therefore it is necessary to assess the set of factors influencing the future of technology. The most important parameters of assessment are acceptance and sustainability of technology. Different models exist, but mostly they are static and do not offer possibilities for interactive and real time assessment and do not allow forecasting the sustainability of technology. In the framework ...

  10. Measuring social risk and determining its acceptability

    The implementation of a nuclear waste management technology raises several issues concerning the regulation of social risk. This paper presents a decision analytic approach to resolving some of those issues. A methodology for developing a radiological risk measure is presented, and several approaches to defining acceptable levels of that risk measure are considered. The methodology presented is oriented toward the development of radiological performance objectives for use as guidance in the drafting of regulations

  11. Heavy fuel oil: acceptable? available? affordable?

    Increasingly stringent environmental legislation on emissions limits poses problems for large industrial energy users, causing them to re-examine their choice of fuel source. Choice of fuel must be made flexibly, preferably in the form of a dual fuel strategy, it is argued here. This article examines the relationship between the 1995 Environment Act, the 1990 Environmental Protection Act, and the likely future of heavy fuel oils in terms of acceptability, availability and affordability. (UK)

  12. Antiseptic technology: access, affordability, and acceptance.

    Boyce, J M

    2001-01-01

    Factors other than antimicrobial activity of soaps and antiseptic agents used for hand hygiene by health personnel play a role in compliance with recommendations. Hand hygiene products differ considerably in acceptance by hospital personnel. If switching from a nonmedicated soap to an antiseptic agent or increased use of an existing antiseptic agent for hand hygiene prevented a few more infections per year, additional expenditures for antiseptic agents would be offset by cost savings.

  13. Acceptance of homosexuality in the Netherlands

    Lisette Kuyper; Floor Bakker

    2006-01-01

    Original title: De houding ten opzichte van homoseksualiteit. To date, relatively little systematic research has been carried out on public attitudes to homosexual men and women in the Netherlands - far less than in the United States, for example. SCP has recently carried out a large-scale survey of the attitudes of the Dutch public to homosexuality; this was published earlier this year under the title Just doing what comes naturally. Acceptance of homosexuality in the Netherlands (Gewoon doe...

  14. Intrinsic work motivation and pension reform acceptance

    Heinemann, Friedrich; Hennighausen, Tanja; Moessinger, Marc-Daniel

    2011-01-01

    Although demographic change leaves pay-as-you-go pension systems unsustainable, reforms, such as a higher pension age, are highly unpopular. This contribution looks into the role of intrinsic motivation as a driver for pension reform acceptance. Theoretical reasoning suggests that this driver should be relevant: The choice among different pension reform options (increasing pension age, increasing contributions, cutting pensions) can be analyzed within the framework of an optimal job separatio...

  15. Consumer studies acceptability on irradiated food

    A questionary to 119 professionals connected with the food field was performed in order to know their attitude, doubts and concernings about food irradiation considering that a favourable opinion would produce trust to the consumer market. The first part of the questionary showed the following results: 13% had never heard about food irradiation (FI), 72% were few familiarized with it, and 14% knew the subject; 42% would accept FI, 37% probably would accept it, 19% could not make up their minds and 2% would not accept it; 45% would eat irradiated food (IF), 45% probably would eat it, 8% probably would not eat it and 2% would not eat it; 44% would serve IF in their home, 45% would probably do so, 8% would probably not do it, and 3% would not do it. The second part showed that 67% of people thought that ionizing radiation (IR) improved the sanitary quality of food, 3% did not think so, and 29% did not know; 63% thought that IR is preferible to chemical preservatives, 4% did not think so and 33% did not know; 11% thought that the food treated with IR becomes radioactive, 60% did not think so, and 29% did not know; 42% thought that FI is wholesome, 8% did not think so, and 50% did not know; 8% consider that the majority of the persons would eat IF, 40% did not think so and 52% did not know; 82% consider necessary that IF have an identificatory label, 10% did not think so, and 8% did not know; 95% consider necessary more diffusion of this method before its commercialization, 2% did not think so and 3% did not know, and 81% want more information, 18% would want it and 1% do not want it. These results are considered to be a good sign of future consumption acceptability of food irradiation. (Author)

  16. Proposals now being accepted for 'INCITE'

    2003-01-01

    Secretary of Energy, Spencer Abraham, has announced that proposals are now being accepted for a new DOE Office of Science program to support innovative, large-scale computational science projects. The program, entitled "Innovative and Novel Computational Impact on Theory and Experiment" will award a total of 4.5 million supercomputer processor hours and 100 trillion bytes of data storage space at the National Energy Research Scientific Computing Center at DOE's Lawrence Berkeley National Laboratory (1 page).

  17. Roulette-wheel selection via stochastic acceptance

    Lipowski, Adam; Lipowska, Dorota

    2011-01-01

    Roulette-wheel selection is a frequently used method in genetic and evolutionary algorithms or in modeling of complex networks. Existing routines select one of N individuals using search algorithms of O(N) or O(log(N)) complexity. We present a simple roulette-wheel selection algorithm, which typically has O(1) complexity and is based on stochastic acceptance instead of searching. We also discuss a hybrid version, which might be suitable for highly heterogeneous weight distributions, found, fo...

  18. Food Authenticity, Technology and Consumer Acceptance

    Hobbs, Jill E.; McDonald, Jillian; Zhang, Jing

    2012-01-01

    Traceability and authenticity issues have gained increasing prominence in food markets and create both opportunities and challenges for the food industry in providing credible information to consumers. Internal molecular tagging is an emerging technology with the potential to deliver traceability and authenticity assurances. A key question for the food industry in adopting new technologies is consumer acceptance. This paper explores consumer attitudes toward traceability and authenticity and ...

  19. Acceptability of male condom: an Indian scenario.

    Donta, Balaiah; Begum, Shahina; Naik, D D

    2014-11-01

    The National Family Planning Programme of India had introduced condom as one of the family planning methods in the late 1960s. Condom was promoted as a family planning method through social marketing since its inception. With the increasing prevalence and incidence of sexually transmitted infections (STIs) including HIV/AIDS, condom was also promoted as a dual method for protection against both unintended pregnancies as well as sexually transmitted infections. Despite efforts at various levels, the overall use of condom among couples in India is low. Here we present literature review of studies to understand the condom acceptability among couples in India. Specifically, the paper assesses research and programmes that have been carried out to increase the use of condom among couples; determinants of condom use; reason for not using condom; and perception versus experience of condom failure. The reported problems related to condom use included non acceptance by partner, perceived ineffectiveness, less comfort, lack of sexual satisfaction, husband's alcohol use, depression, and anxiety, and not available at that instant. The role of media in the promotion of condom use was indicated as an important way to increase awareness and use. Multiple strategies would help in acceptance of male condom. PMID:25673537

  20. Rationality, Theory Acceptance and Decision Theory

    J. Nicolas Kaufmann

    1998-06-01

    Full Text Available Following Kuhn's main thesis according to which theory revision and acceptance is always paradigm relative, I propose to outline some possible consequences of such a view. First, asking the question in what sense Bayesian decision theory could serve as the appropriate (normative theory of rationality examined from the point of view of the epistemology of theory acceptance, I argue that Bayesianism leads to a narrow conception of theory acceptance. Second, regarding the different types of theory revision, i.e. expansion, contraction, replacement and residuals shifts, I extract from Kuhn's view a series of indications showing that theory replacement cannot be rationalized within the framework of Bayesian decision theory, not even within a more sophisticated version of that model. Third, and finally, I will point to the need for a more comprehensive model of rationality than the Bayesian expected utility maximization model, the need for a model which could better deal with the different aspects of theory replacement. I will show that Kuhn's distinction between normal and revolutionary science gives us several hints for a more adequate theory of rationality in science. I will also show that Kuhn is not in a position to fully articulate his main ideas and that he well be confronted with a serious problem concerning collective choice of a paradigm.

  1. A structural model of technology acceptance

    Etienne Erasmus

    2015-02-01

    Full Text Available Orientation: Enterprise resource systems have not always led to significant organisational enhancement and many projects in which these systems have been implemented turn out to be over budget, not on time and unsuccessful.Research purpose: The aim of this study was to test the technology acceptance model within a South African SAP® Enterprise Resource Planning user environment.Motivation for the study: No study could be traced in which the technology acceptance model has been evaluated in the South African context.Research approach, design and method: A cross-sectional survey design was used. The 23-item Technology Acceptance Model Questionnaire was deployed amongst SAP® Enterprise Resource Planning users (N = 241. Main findings: The results confirmed significant paths from perceived usefulness of the information system to attitudes towards and behavioural intentions to use it. Furthermore, behavioural intention to use the system predicted actual use thereof. Perceived ease of use indirectly affected attitudes towards and behavioural intentions to use via perceived usefulness of the information system.Practical/managerial implications: Practitioners should build user confidence by ensuring the ease of use of a new system, providing relevant education, training and guidance and reiterating its usefulness and future added value to the user’s job and career.Contribution/value-add: This study contributes to scientific knowledge regarding the influence of individuals’ perceptions of information system usage on their attitudes, behavioural intentions and actual use of such a system.

  2. Acceptable regret in medical decision making.

    Djulbegovic, B; Hozo, I; Schwartz, A; McMasters, K M

    1999-09-01

    When faced with medical decisions involving uncertain outcomes, the principles of decision theory hold that we should select the option with the highest expected utility to maximize health over time. Whether a decision proves right or wrong can be learned only in retrospect, when it may become apparent that another course of action would have been preferable. This realization may bring a sense of loss, or regret. When anticipated regret is compelling, a decision maker may choose to violate expected utility theory to avoid regret. We formulate a concept of acceptable regret in medical decision making that explicitly introduces the patient's attitude toward loss of health due to a mistaken decision into decision making. In most cases, minimizing expected regret results in the same decision as maximizing expected utility. However, when acceptable regret is taken into consideration, the threshold probability below which we can comfortably withhold treatment is a function only of the net benefit of the treatment, and the threshold probability above which we can comfortably administer the treatment depends only on the magnitude of the risks associated with the therapy. By considering acceptable regret, we develop new conceptual relations that can help decide whether treatment should be withheld or administered, especially when the diagnosis is uncertain. This may be particularly beneficial in deciding what constitutes futile medical care. PMID:10580533

  3. Public acceptance of nuclear energy in Indonesia

    In Indonesia, the activities in nuclear field have developed since 1954 when a government committee was formed. This committee had the task to carry out nuclear energy research, and after this committee reported its work, the Lembaga Tenaga Atom (Agency for Atomic Energy) was founded in 1958, and in 1964, it became Badan Tenaga Atom Nasional through the Basic Act on Atomic Energy. BATAN is a government agency, of which the main tasks are the development of nuclear energy by regulating, administering and controlling all nuclear activities in Indonesia and conducting the research and development of the use of nuclear energy. One of its tasks is to give general and technical informations on nuclear technology to public, and the activities on public acceptance started at the end of 1985. The feasibility study to introduce the first nuclear power plant has been done by a Japanese NEWJEC Inc., and completed in May, 1996. The public acceptance program and strategy and the activities of the public acceptance of a nuclear power plant are reported. The results of the public socialization and exhibition can be seen by the increasing number of the participants in the programs. (K.I.)

  4. Acceptability of male condom: An Indian scenario

    Balaiah Donta

    2014-01-01

    Full Text Available The National Family Planning Programme of India had introduced condom as one of the family planning methods in the late1960s. Condom was promoted as a family planning method through social marketing since its inception. With the increasing prevalence and incidence of sexually transmitted infections (STIs including HIV/AIDS, condom was also promoted as a dual method for protection against both unintended pregnancies as well as sexually transmitted infections. Despite efforts at various levels, the overall use of condom among couples in India is low. Here we present literature review of studies to understand the condom acceptability among couples in India. Specifically, the paper assesses research and programmes that have been carried out to increase the use of condom among couples; determinants of condom use; reason for not using condom; and perception versus experience of condom failure. The reported problems related to condom use included non acceptance by partner, perceived ineffectiveness, less comfort, lack of sexual satisfaction, husband′s alcohol use, depression, and anxiety, and not available at that instant. The role of media in the promotion of condom use was indicated as an important way to increase awareness and use. Multiple strategies would help in acceptance of male condom.

  5. 48 CFR 552.270-29 - Acceptance of Space.

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Acceptance of Space. 552... Acceptance of Space. As prescribed in 570.603, insert the following clause: Acceptance of Space (SEP 1999) (a... designated representative shall promptly inspect the space. (b) The Government will accept the space and...

  6. Perceptions of Social Behavior and Peer Acceptance in Kindergarten.

    Phillipsen, Leslie C.; Bridges, Sara K.; McLemore, T. Gayle; Saponaro, Lisa A.

    1999-01-01

    Used social behavior ratings from observers, teachers, and parents to predict kindergartners' perceptions of peer acceptance. Found that friendship skill predicted parent- and child-reported peer acceptance. Shyness/withdrawal inversely predicted teacher-reported peer acceptance. Aggression did not predict peer acceptance. Girls were rated as more…

  7. 48 CFR 28.203-2 - Acceptability of assets.

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Acceptability of assets... Acceptability of assets. (a) The Government will accept only cash, readily marketable assets, or irrevocable... underlying bond obligations. (b) Acceptable assets include— (1) Cash, or certificates of deposit, or...

  8. Dogmatism, Self-Acceptance, and Acceptance of Others Among Spanish and American Students

    Gonzalez-Tamayo, Eulogy

    1974-01-01

    A cross cultural study of 16-year-olds in Spain and the United States assesses the extent to which open or closed societies effect performance on the Dogmatism Scale, and the Acceptance of Self and Others Attitude Scale. (JH)

  9. Evaluation of the Acceptance of Audience Response System by Corporations Using the Technology Acceptance Model

    Chu, Hsing-Hui; Lu, Ta-Jung; Wann, Jong-Wen

    The purpose of this research is to explore enterprises' acceptance of Audience Response System (ARS) using Technology Acceptance Model (TAM). The findings show that (1) IT characteristics and facilitating conditions could be external variables of TAM. (2) The degree of E-business has positive significant correlation with behavioral intention of employees. (3) TAM is a good model to predict and explain IT acceptance. (4) Demographic variables, industry and firm characteristics have no significant correlation with ARS acceptance. The results provide useful information to managers and ARS providers that (1) ARS providers should focus more on creating different usages to enhance interactivity and employees' using intention. (2) Managers should pay attention to build sound internal facilitating conditions for introducing IT. (3) According to the degree of E-business, managers should set up strategic stages of introducing IT. (4) Providers should increase product promotion and also leverage academic and government to promote ARS.

  10. DNA modifications: Another stable base in DNA

    Brazauskas, Pijus; Kriaucionis, Skirmantas

    2014-12-01

    Oxidation of 5-methylcytosine has been proposed to mediate active and passive DNA demethylation. Tracking the history of DNA modifications has now provided the first solid evidence that 5-hydroxymethylcytosine is a stable epigenetic modification.

  11. How is Acceptable Public Risk Determined?

    Treichel, Judy [Nevada Nuclear Waste Task Force, Las Vegas, NV (United States)

    2001-07-01

    Acceptance of risk is a value-based decision, that is, the acceptance of risk by a person or group of persons depends on the values of the person or the shared values of the group. In the case of nuclear waste management, the nuclear industry, the regulators, and the general public approach risk from entirely different perspectives, dictated by the separate value systems held by each. The utilities producing radioactive waste view risk assessment as a part of a business decision that involves costs and benefits. The values that drive public acceptance of a national nuclear waste management policy are very different. As stated by Peter Montague of the Environmental Research Foundation: 'The only people I know who are enthusiastic about quantitative risk assessment are people who want to gain permission to expose other humans to dangerous chemicals so someone can make money. Risk assessment has proven to be an effective way to gain the necessary permissions'. Between the industry and the public are the regulators. Most national governments require regulatory agencies to establish rules that provide adequate public safety while allowing industries, whether nuclear or other producers of public commodities, to profitably do business. The general population has always had a fragile relationship with nuclear proponents. There is an atmosphere of mistrust based on the understanding that the values that matter to the general public differ tremendously from those purported by the industry and regulators. The general public is more interested in worst case scenarios; that is, what is the most severe negative consequence to their safety and the safety of their children that could result from nuclear projects. There is no cost or benefit more important to the general public than the health and safety of their families. The rift in values creates a great disparity in proposed solutions to the nuclear waste question. Regulators regard public acceptance of a risk

  12. How is Acceptable Public Risk Determined?

    Acceptance of risk is a value-based decision, that is, the acceptance of risk by a person or group of persons depends on the values of the person or the shared values of the group. In the case of nuclear waste management, the nuclear industry, the regulators, and the general public approach risk from entirely different perspectives, dictated by the separate value systems held by each. The utilities producing radioactive waste view risk assessment as a part of a business decision that involves costs and benefits. The values that drive public acceptance of a national nuclear waste management policy are very different. As stated by Peter Montague of the Environmental Research Foundation: 'The only people I know who are enthusiastic about quantitative risk assessment are people who want to gain permission to expose other humans to dangerous chemicals so someone can make money. Risk assessment has proven to be an effective way to gain the necessary permissions'. Between the industry and the public are the regulators. Most national governments require regulatory agencies to establish rules that provide adequate public safety while allowing industries, whether nuclear or other producers of public commodities, to profitably do business. The general population has always had a fragile relationship with nuclear proponents. There is an atmosphere of mistrust based on the understanding that the values that matter to the general public differ tremendously from those purported by the industry and regulators. The general public is more interested in worst case scenarios; that is, what is the most severe negative consequence to their safety and the safety of their children that could result from nuclear projects. There is no cost or benefit more important to the general public than the health and safety of their families. The rift in values creates a great disparity in proposed solutions to the nuclear waste question. Regulators regard public acceptance of a risk-informed policy

  13. HPV vaccination acceptability in young boys

    Giancarlo Tisi

    2013-09-01

    Full Text Available PURPOSE: The aim of this study was to evaluate the comprehension and acceptance of HPV vaccination in parents of adolescent boys aged 11 to 15 years. METHODS: A cross-sectional survey was conducted by means of questionnaires sent directly to the homes of all families with young males aged between 11 and 15, residents of three municipalities of the Province of Brescia, Italy. The documentation also contained an informative leaflet summarizing the HPV-related disease characteristics, the burden of disease and the available strategies for prevention and treatment, illustrating the rationale of vaccination and describing the project and its phases. The questionnaire included questions on demographic data, acceptance and motivations for HPV vaccination. The collected data was analyzed using descriptive statistics. At the end of the study, parents who received the questionnaires were also offered the possibility of vaccinating their male sons for free. RESULTS: From a total of 1072 questionnaires sent, 161 where returned from the three selected municipalities (average response rate 15%; 97% of adolescent males involved in the study were Italian and 91% Catholic; 97% of parents declared themselves to be willing to vaccinate their sons: the principal motivation given (92% was prevention of the disease, cancerous or not, related to viral infection. Among the respondents not willing to vaccinate their sons, the motivation was lack of information about the vaccine and the disease. At the end of the study, around 71 boys were vaccinated. DISCUSSION: To our knowledge, this is the first survey in Italy exclusively conducted on parents of adolescent males about the acceptability and feasibility of vaccination against HPV: a very high percentage of respondents was favorable to accept the vaccination for their sons, the main motivation being the fact that parents considered protecting their sons from HPV-related diseases highly important. Of the 161 boys

  14. Predicting Public Acceptability in Controversial Technologies

    Technology and society have a synergic relationship. New technologies are a product of the values and aspirations of the culture in which they emerge. In turn, each new technology shapes and alters these values and aspirations, to a greater or lesser degree. The acceptance by society of any particular technology depends, however, on how far the values embodied in the technology reflect those of the wider society, or only those of some privileged sector - perhaps a ruling elite, a group of academic researchers, a commercial company, or even a special interest group. As public disquiet about controversial technologies has grown, their acceptance can no longer be taken for granted. It is now becoming more important to evaluate in advance the degree of likely mismatch between the aspirations of the technologists and the values of society. This paper explores one approach to making this evaluation, based on the notion of a conditional social contract between technology and society. A given society may be prepared to embrace a new technology to deliver certain benefits, and may accept a certain degree of risk and adaptation of life styles, provided certain basic conditions are fulfilled. These conditions include the upholding of basic values, familiarity, how it compares with similar technologies, the degree of control and choice, trust in those in control, the nature of any risks, the tangible benefits, and the media profile given to the new area. If several of these factors are not fulfilled, the technology is unlikely to be accepted. This was dramatically illustrated in the UK public reaction to food products derived from imported US GM soya and maize. These failed nearly all the conditions, so that public rejection should have been seen as a foregone conclusion. In the light of this, the likely public reaction to a number of future biotechnological innovations is assessed, based on the same conditions. Some examples taken from the energy sector are also compared. The

  15. Identification of permit and waste acceptance criteria provisions requiring modification for acceptance of commercial mixed waste

    In October 1990, representatives of States and compact regions requested that the US Department of Energy (DOE) explore an agreement with host States and compact regions under which DOE would accept commercial mixed low-level radioactive waste (LLW) at DOE's own treatment and disposal facilities. A program for DOE management of commercial mixed waste is made potentially more attractive in light of the low commercial mixed waste volumes, high regulatory burdens, public opposition to new disposal sites, and relatively high cost of constructing commercial disposal facilities. Several studies were identified as essential in determining the feasibility of DOE accepting commercial mixed waste for disposal. The purpose of this report is to identify any current or proposed waste acceptance criteria (WAC) or Resource Conservation and Recovery Act (RCRA) provisions that would have to be modified for commercial mixed waste acceptance at specified DOE facilities. Following the introduction, Section 2 of this report (a) provides a background summary of existing and proposed mixed waste disposal facilities at each DOE site, and (b) summarizes the status of any RCRA Part B permit and WAC provisions relating to the disposal of mixed waste, including provisions relating to acceptance of offsite waste. Section 3 provides overall conclusions regarding the current status and permit modifications that must be implemented in order to grant DOE sites authority under their permits to accept commercial mixed waste for disposal. Section 4 contains a list of references

  16. Synthesis of DNA

    Mariella, Jr., Raymond P.

    2008-11-18

    A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined sequence. Preselected sequence segments that will complete the desired double-stranded DNA are determined. Preselected segment sequences of DNA that will be used to complete the desired double-stranded DNA are provided. The preselected segment sequences of DNA are assembled to produce the desired double-stranded DNA.

  17. Public Acceptance of Nuclear Energy in Mexico

    The nuclear energy is attracting renewed interest of public and policy makers due to his potential role in long term strategies aiming to reduce the risk of global warming and in a more general, to carry out sustainable policies, however, any project of nuclear nature arise concerns about the risks associated with the release of radioactivity during accident conditions, radioactive waste disposal and nuclear weapons proliferation. Then in light of the likeliness for a new nuclear project in Mexico, is necessary to design a strategy to improve the social acceptance of nuclear power. This concern is been boarding since the environmental and economic point of view. The information that can change the perception of nuclear energy towards increase public acceptance, should be an honest debate about the benefits of nuclear energy, of course there are questions and they have to be answered, but in a realistic and scientific way: So thinking in Mexico as a first step it is important to communicate to the government entities and political parties that nuclear energy is a proven asset that it is emission free and safe. Of course besides the guarantee of a proven technology, clean and safe relies the economic fact, and in Mexico this could be the most important aspect to communicate to key people in government. Based in the Laguna Verde survey it is clear that we have to find the adequate means to distribute the real information concerning nuclear technology to the public, because the results shows that Mexican people does not have complete information about nuclear energy, but public can support it when they have enough information. From the IAEA study we can say that in Mexico public acceptance of nuclear energy it s not so bad, is the highest percentage of acceptance of nuclear technology for health, considering benefits to the environment Mexican opposition to build new plants is the second less percentage, and generally speaking 60% of the people accept somehow nuclear

  18. Sperm DNA oxidative damage and DNA adducts.

    Jeng, Hueiwang Anna; Pan, Chih-Hong; Chao, Mu-Rong; Lin, Wen-Yi

    2015-12-01

    The objective of this study was to investigate DNA damage and adducts in sperm from coke oven workers who have been exposed to polycyclic aromatic hydrocarbons. A longitudinal study was conducted with repeated measurements during spermatogenesis. Coke-oven workers (n=112) from a coke-oven plant served the PAH-exposed group, while administrators and security personnel (n=67) served the control. Routine semen parameters (concentration, motility, vitality, and morphology) were analyzed simultaneously; the assessment of sperm DNA integrity endpoints included DNA fragmentation, bulky DNA adducts, and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo). The degree of sperm DNA fragmentation was measured using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay and sperm chromatin structure assay (SCSA). The PAH-exposed group had a significant increase in bulky DNA adducts and 8-oxo-dGuo compared to the control subjects (Ps=0.002 and 0.045, respectively). Coke oven workers' percentages of DNA fragmentation and denaturation from the PAH-exposed group were not significantly different from those of the control subjects (Ps=0.232 and 0.245, respectively). Routine semen parameters and DNA integrity endpoints were not correlated. Concentrations of 8-oxo-dGuo were positively correlated with percentages of DNA fragmentation measured by both TUNEL and SCSA (Ps=0.045 and 0.034, respectively). However, the concentrations of 8-oxo-dGuo and percentages of DNA fragmentation did not correlate with concentrations of bulky DNA adducts. In summary, coke oven workers with chronic exposure to PAHs experienced decreased sperm DNA integrity. Oxidative stress could contribute to the degree of DNA fragmentation. Bulky DNA adducts may be independent of the formation of DNA fragmentation and oxidative adducts in sperm. Monitoring sperm DNA integrity is recommended as a part of the process of assessing the impact of occupational and environmental toxins on sperm

  19. DNA glycosylases: in DNA repair and beyond

    Jacobs, Angelika L.; Schär, Primo

    2011-01-01

    The base excision repair machinery protects DNA in cells from the damaging effects of oxidation, alkylation, and deamination; it is specialized to fix single-base damage in the form of small chemical modifications. Base modifications can be mutagenic and/or cytotoxic, depending on how they interfere with the template function of the DNA during replication and transcription. DNA glycosylases play a key role in the elimination of such DNA lesions; they recognize and excise damaged bases, thereb...

  20. DNA vaccines and bacterial DNA in immunity

    Bandholtz, Lisa Charlotta

    2002-01-01

    This thesis describes DNA-based vaccination and the importance of bacterial DNA in different immunological perspectives. Intranasal (i.n.) DNA vaccination utilizing a plasmid encoding the chlamydial heat shock protein 60 (p-hsp-60) generated lower bacterial burden and reduced pathology in the lungs of mice after subsequent infection with C. pneumoniae. This DNA vaccine- induced protection was dependent on T cells and induction of IFN-gamma. Co-administration of a plasmid...

  1. DNA encoding a DNA repair protein

    Petrini, John H.; Morgan, William Francis; Maser, Richard Scott; Carney, James Patrick

    2006-08-15

    An isolated and purified DNA molecule encoding a DNA repair protein, p95, is provided, as is isolated and purified p95. Also provided are methods of detecting p95 and DNA encoding p95. The invention further provides p95 knock-out mice.

  2. Dynamic DNA cytosine methylation in the Populus trichocarpa genome: tissue-level variation and relationship to gene expression

    Vining Kelly J

    2012-01-01

    Full Text Available Abstract Background DNA cytosine methylation is an epigenetic modification that has been implicated in many biological processes. However, large-scale epigenomic studies have been applied to very few plant species, and variability in methylation among specialized tissues and its relationship to gene expression is poorly understood. Results We surveyed DNA methylation from seven distinct tissue types (vegetative bud, male inflorescence [catkin], female catkin, leaf, root, xylem, phloem in the reference tree species black cottonwood (Populus trichocarpa. Using 5-methyl-cytosine DNA immunoprecipitation followed by Illumina sequencing (MeDIP-seq, we mapped a total of 129,360,151 36- or 32-mer reads to the P. trichocarpa reference genome. We validated MeDIP-seq results by bisulfite sequencing, and compared methylation and gene expression using published microarray data. Qualitative DNA methylation differences among tissues were obvious on a chromosome scale. Methylated genes had lower expression than unmethylated genes, but genes with methylation in transcribed regions ("gene body methylation" had even lower expression than genes with promoter methylation. Promoter methylation was more frequent than gene body methylation in all tissues except male catkins. Male catkins differed in demethylation of particular transposable element categories, in level of gene body methylation, and in expression range of genes with methylated transcribed regions. Tissue-specific gene expression patterns were correlated with both gene body and promoter methylation. Conclusions We found striking differences among tissues in methylation, which were apparent at the chromosomal scale and when genes and transposable elements were examined. In contrast to other studies in plants, gene body methylation had a more repressive effect on transcription than promoter methylation.

  3. Use of DNA technology in forensic dentistry

    Ricardo Henrique Alves da Silva; Arsenio Sales-Peres; Rogério Nogueira Oliveira; Fernando Toledo de Oliveira; Sílvia Helena de Carvalho Sales-Peres

    2007-01-01

    The established importance of Forensic Dentistry for human identification, mainly when there is little remaining material to perform such identification (e.g., in fires, explosions, decomposing bodies or skeletonized bodies), has led dentists working with forensic investigation to become more familiar with the new molecular biology techniques. The currently available DNA tests have high reliability and are accepted as legal proofs in courts. This article presents a literature review referring...

  4. Transposable Elements: No More 'Junk DNA'

    Kim, Yun-Ji; Lee, Jungnam; Han, Kyudong

    2012-01-01

    Since the advent of whole-genome sequencing, transposable elements (TEs), just thought to be 'junk' DNA, have been noticed because of their numerous copies in various eukaryotic genomes. Many studies about TEs have been conducted to discover their functions in their host genomes. Based on the results of those studies, it has been generally accepted that they have a function to cause genomic and genetic variations. However, their infinite functions are not fully elucidated. Through various mec...

  5. An international comparative analysis of public acceptance of nuclear energy

    Across the globe, public acceptance of nuclear power is a crucial factor for governmental establishment of a nuclear energy program. Therefore, it is important to understand the determinants of public acceptance of nuclear power. This study examines the effects of knowledge, trust, risk, and benefit related factors on public acceptance of nuclear power across 19 countries. We consider three levels of public acceptance – strongly accept, reluctantly accept, and oppose – and classify countries into four groups according to the ratio of those three levels of public acceptance. Our results indicate that knowledge of nuclear inspection is more effective than trust in inspection authorities in creating stronger public acceptance among people in the countries with a high level of reluctant acceptance and a low level of strong acceptance, while trust in inspection authorities is more important than knowledge of nuclear inspection for the selection between opposition and reluctant acceptance in countries with a low level of reluctant acceptance and a high level of strong acceptance. Without grouping the countries, we found that trust in inspection authorities is crucial for the decision between opposition and reluctant acceptance. Additionally, the generation of electricity has the most positive effect on public acceptance of nuclear power. - Highlights: • We examine public acceptance (PA) of nuclear power across 19 countries. • Three levels of PA – strongly accept, reluctantly accept, and oppose – are considered. • Knowledge is most effective in creating stronger PA. • Trust is effective in shifting PA from opposition to reluctant acceptance. • Low risk and benefit of electricity generation enhance PA the most

  6. Global Acceptance Programs towards Coastal States

    The transport by sea of highly radioactive materials such as used fuel and vitrified waste has elicited concerns in some States along the shipping routes. In order to alleviate those concerns, three industry members from Japan, the United Kingdom and France have introduced Global Acceptance programs towards these 'Coastal States'. These partners had the mission to manage nuclear transports through Japan under the strictest safety and security measures and with the acceptance of the coastal states alongside the maritime routes used between Europe and Japan. During these transports they have to face some strong opposition from states, anti nuclear NGOs acting during the transports or inside the local and international fora. The Global Acceptance team has developed a unique approach to allow these transports to be performed normally all around the world. Organizing missions in the relevant countries to explain our transports and our safety and security measures, inviting top authorities to visit our ships and facilities in Europe, dispatching up to date files and communication documents, lobbying the top organizations and executives of these countries, developing a long time relationship with local authorities, looking for relevant relay inside the educational world - were part of the business dedicated to - keep our routes open - for the benefit of our industries. During these mission we have discovered that ignorance, misinformation, lack of knowledge in a difficult environment can lead countries, NGOs, political bodies, international fora to take irrelevant positions about transports which all comply with the strongest international requirements in term of safety and security. It was a unique experience for the benefit of our companies and we have now to check again with an on going program in the future where we will get the experience gained during the past 17 years to complete these contracts. (author)

  7. Public acceptance of nuclear energy in Mexico

    One of the main constraints to adopt a nuclear program is the public acceptance. In Mexico, at least, it lacks of an adequate promotion of its benefits and challenges. A big stigma for nuclear electricity production is the association with nuclear weapons, along with myths and misconceptions and bad information about nuclear energy. Mexico has adopted an energy policy to diversify the electricity sources and nuclear energy is among the alternatives to achieve this goal because current studies show that is a safe and a competitive option from an economical point of view. Public opinion plays a very important role in the policy decision making to adopt the deployment of new reactor units; therefore it is necessary to define communication strategies to promote nuclear energy. The current study is an investigation to learn what is the perception and positioning about nuclear energy as a starting point to define the way to improve public acceptance. The national assessment carry on here is divided in two parts, the first one is a qualitative study to know knowledge level, associations and nuclear perception, identifying controversy items and expectations about advantages and disadvantages to define the adequate question to be used in the second part, which is a quantitative study that shows the acceptance of nuclear energy at national level and in particular in two sites that are suitable to deploy new nuclear reactors. From the results of this study some communication and persuasion strategies to improve public perception are defined and they could be used as part of a nuclear program. (author)

  8. Accepting, understanding, teaching, and learning (human) evolution: Obstacles and opportunities.

    Pobiner, Briana

    2016-01-01

    Questions about our origin as a species are universal and compelling. Evolution-and in particular human evolution-is a subject that generates intense interest across the world, evidenced by the fact that fossil and DNA discoveries grace the covers of major science journals and magazines as well as other popular print and online media. However, virtually all national polls indicate that the majority of Americans strongly reject biological evolution as a fact-based, well-tested, and robust understanding of the history of life. In the popular mind, no topic in all of science is more contentious or polarizing than evolution and media sources often only serve to magnify this polarization by covering challenges to the teaching of evolution. In the realm of teaching, debates about evolution have shaped textbooks, curricula, standards, and policy. Challenges to accepting and understanding evolution include mistrust and denial of science, cognitive obstacles and misconceptions, language and terminology, and a religious worldview, among others. Teachers, who are on the front lines of these challenges, must be armed with the tools and techniques to teach evolution in formal education settings across grades K-16 in a straightforward, thorough, and sensitive way. Despite the potentially controversial topic of human evolution, growing research is demonstrating that a pedagogical focus on human examples is an effective and engaging way to teach core concepts of evolutionary biology. Am J Phys Anthropol 159:S232-S274, 2016. © 2016 Wiley Periodicals, Inc. PMID:26808108

  9. Acceptability of the standard cosmological model

    An attempt is made to assess the acceptability of the standard model of cosmology. While the success claimed in connection with the microwave background radiation and primordial nucleogenesis may not be convincing, the absence of any adhoc hypothesis in the standard model and the nonavailability of any better model are strong arguments in its favour. It is suggested that anomalous redshifts and the quantal effect in the redshifts are as yet little understood and do not invalidate the idea of redshift due to universal expansion. (author)

  10. Improving public acceptance of Nuclear Energy

    Full text: After the Chernobyl accident in 1986, public acceptance of nuclear energy decreased dramatically. In order to prevent such catastrophic events, major safety improvements have been achieved both in the operating nuclear power plants and in the future advanced projects as well. Nowadays, the nuclear energy problem was totally modified by the complete mastery and control of nuclear power generation as well as of radioactive waste management. It become a solution to the global climatic challenges and equally to the ever increasing world energy demand. (author)

  11. Study on public acceptance of irradiated potatoes

    4,500 kg potatoes of different sorts were irradiated by a 137Cs source with a dose of 10 krad. The potatoes were then stored for several months at a room temperature of 6 to 100C and a humidity of 80 to 85%. 0% of the irradiated potatoes sprouted, as compared to 100% of the non-irradiated potatoes. The percentage of rotting varied in the irradiated potatoes, depending on the variety. Cooking tests showed no difference between irradiated and non-irradiated potatoes. The irradiated potatoes were consumed and accepted by about 4,000 persons in the cafeteria of the institute. (AJ)

  12. Development of quantitative risk acceptance criteria

    Some of the major considerations for effective management of risk are discussed, with particular emphasis on risks due to nuclear power plant operations. Although there are impacts associated with the rest of the fuel cycle, they are not addressed here. Several previously published proposals for quantitative risk criteria are reviewed. They range from a simple acceptance criterion on individual risk of death to a quantitative risk management framework. The final section discussed some of the problems in the establishment of a framework for the quantitative management of risk

  13. W-026, acceptance test report manipulator system

    Watson, T.L.

    1997-04-15

    The purpose of the WRAP Manipulator System Acceptance Test Plan (ATP) is to verify that the 4 glovebox sets of WRAP manipulator components, including rail/carriage, slave arm, master controller and auxiliary equipment, meets the requirements of the functional segments of 14590 specification. The demonstration of performance elements of the ATP are performed as a part of the Assembly specifications. Manipulator integration is integrated in the performance testing of the gloveboxes. Each requirement of the Assembly specification will be carried out in conjunction with glovebox performance tests.

  14. Roulette-wheel selection via stochastic acceptance

    Lipowski, Adam

    2011-01-01

    Roulette-wheel selection is a frequently used method in genetic or evolutionary algorithms. Existing routines select one of N individuals using search algorithms of O(N) or O(\\log N) complexity. We present a simple roulette-wheel selection algorithm that typically has O(1) complexity and is based on stochastic acceptance instead of searching. With minor modifications the algorithm might be also used for sampling with fitness being cut-off at a certain value or for sampling without replacement.

  15. Roulette-wheel selection via stochastic acceptance

    Lipowski, Adam; Lipowska, Dorota

    2012-03-01

    Roulette-wheel selection is a frequently used method in genetic and evolutionary algorithms or in modeling of complex networks. Existing routines select one of N individuals using search algorithms of O(N) or O(logN) complexity. We present a simple roulette-wheel selection algorithm, which typically has O(1) complexity and is based on stochastic acceptance instead of searching. We also discuss a hybrid version, which might be suitable for highly heterogeneous weight distributions, found, for example, in some models of complex networks. With minor modifications, the algorithm might also be used for sampling with fitness cut-off at a certain value or for sampling without replacement.

  16. The interferon response to intracellular DNA: why so many receptors?

    Unterholzner, Leonie

    2013-11-01

    The detection of intracellular DNA has emerged to be a key event in the innate immune response to viruses and intracellular bacteria, and during conditions of sterile inflammation and autoimmunity. One of the consequences of the detection of DNA as a 'stranger' and a 'danger' signal is the production of type I interferons and pro-inflammatory cytokines. Much work has been dedicated to the elucidation of the signalling cascades that activate this DNA-induced gene expression programme. However, while many proteins have been proposed to act as sensors for intracellular DNA in recent years, none has been met with universal acceptance, and a theory linking all the recent observations is, as yet, lacking. This review presents the evidence for the various interferon-inducing DNA receptors proposed to date, and examines the hypotheses that might explain why so many different receptors appear to be involved in the innate immune recognition of intracellular DNA. PMID:23962476

  17. Capstan friction model for DNA ejection from bacteriophages

    Ghosal, Sandip

    2013-01-01

    Bacteriophages infect cells by attaching to the outer membrane and injecting their DNA into the cell.The phage DNA is then transcribed by the cell's transcription machinery.A number of physical mechanisms by which DNA can be translocated from the phage capsid into the cell have been identified. A fast ejection driven by the elastic and electrostatic potential energy of the compacted DNA within the viral capsid appears to be used by most phages, at least to initiate infection.In recent in vitro experiments, the speed of DNA translocation from a lambda phage capsid has been measured as a function of ejected length over the entire duration of the event.Here a mechanical model is proposed that is able to explain the observed dependence of exit velocity on ejected length, and that is also consistent with the accepted picture of the geometric arrangement of DNA within the viral capsid.

  18. Human placental DNA methyltransferase: DNA substrate and DNA binding specificity.

    Wang, R.Y.; Huang, L. H.; Ehrlich, M

    1984-01-01

    We have partially purified a DNA methyltransferase from human placenta using a novel substrate for a highly sensitive assay of methylation of hemimethylated DNA. This substrate was prepared by extensive nick translation of bacteriophage XP12 DNA, which normally has virtually all of its cytosine residues replaced by 5-methylcytosine (m5C). Micrococcus luteus DNA was just as good a substrate if it was first similarly nick translated with m5dCTP instead of dCTP in the polymerization mixture. At ...

  19. Are all GMOs the same? Consumer acceptance of cisgenic rice in India.

    Shew, Aaron M; Nalley, Lawton L; Danforth, Diana M; Dixon, Bruce L; Nayga, Rodolfo M; Delwaide, Anne-Cecile; Valent, Barbara

    2016-01-01

    India has more than 215 million food-insecure people, many of whom are farmers. Genetically modified (GM) crops have the potential to alleviate this problem by increasing food supplies and strengthening farmer livelihoods. For this to occur, two factors are critical: (i) a change in the regulatory status of GM crops, and (ii) consumer acceptance of GM foods. There are generally two classifications of GM crops based on how they are bred: cisgenically bred, containing only DNA sequences from sexually compatible organisms; and transgenically bred, including DNA sequences from sexually incompatible organisms. Consumers may view cisgenic foods as more natural than those produced via transgenesis, thus influencing consumer acceptance. This premise was the catalyst for our study--would Indian consumers accept cisgenically bred rice and if so, how would they value cisgenics compared to conventionally bred rice, GM-labelled rice and 'no fungicide' rice? In this willingness-to-pay study, respondents did not view cisgenic and GM rice differently. However, participants were willing-to-pay a premium for any aforementioned rice with a 'no fungicide' attribute, which cisgenics and GM could provide. Although not significantly different (P = 0.16), 76% and 73% of respondents stated a willingness-to-consume GM and cisgenic foods, respectively. PMID:26242818

  20. The acceptability of nanocarriers for drug delivery in different contexts of use: perceptions of researchers and research trainees in the field of new technologies

    Chenel V

    2015-03-01

    Full Text Available Vanessa Chenel,1–4 Patrick Boissy,1–3 Marie-Sol Poirier,1–3 Jean-Pierre Cloarec,3,4 Johane Patenaude1–3 1Interdisciplinary Institute for Technological Innovation (3IT, 2Department of Surgery, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada; 3Laboratoire Nanotechnologies et Nanosystèmes (LN2, Centre National de la Recherche Scientifique (CNRS, Université de Sherbrooke, Canada; 4Université de Lyon, Institut des Nanotechnologies de Lyon (INL, site École Centrale de Lyon, France Background: Despite marked optimism in the field of nanomedicine about the use of drug-delivery nanocarriers, uncertainties exist concerning nanocarriers’ possible unintended impacts and effects. These uncertainties could affect user acceptance and acceptability. “Acceptance” refers to the intention to put a technology or a device to a specified use. “Acceptability” refers to a value judgment that accounts for acceptance. The objectives of this study were to characterize impact perception, acceptance, and acceptability in relation to drug-delivery nanocarriers in different contexts of use, and to explore relationships among these concepts.Methods: A sample of European and Canadian researchers and graduate research trainees active in the field of new technologies was recruited by targeted email invitation for participation in a web-based questionnaire study. The questionnaire presented scenarios for two contexts of use (lung cancer, seasonal flu of drug-delivery nanocarriers with two compositions (carbon, synthetic DNA. Respondents’ impact perception, acceptance, and acceptability judgment in relation to each kind of nanocarrier in each context of use were measured with Likert scale questions and scored using categorical values.Results: Two hundred and fourteen researchers and graduate research trainees completed the questionnaire. The results showed that nanocarrier composition influenced impact perception

  1. Environmentally acceptable drill pipe thread compound

    Environmental concerns are prompting the petroleum industry to replace thread compounds. Traditional thread compound formulations, now widely used for more than three decades have proven effective. But all contain significant percentages (up to about 60%) of heavy metal powders. Ingredients such as copper, lead and zinc are increasingly being banned for use or dumping. This paper outlines the issues and describes the new technology which will be required in most areas during 1992. It introduces a drill pipe thread compound with unique mechanisms to achieve improved functional performance while providing environmental acceptability. Rotary shouldered compounds (and orthodox thinking) have relied upon soft, low melting point, malleable metal particles to provide connection sealing and anti-galling characteristics. The new approach uses the non-conformist approach of hard ceramic microspheres to achieve the same results. This work gives the application and general performance requirements for rotary shouldered thread compounds. It compares the new technology with traditional compounds from the standpoint of composition, functional performance, and environmental acceptability. Bomac's work now makes it possible to run a drilling project entirely free of lead, copper and zinc

  2. Steam generator tube integrity flaw acceptance criteria

    Cochet, B. [FRAMATOME, Paris la Defense (France)

    1997-02-01

    The author discusses the establishment of a flaw acceptance criteria with respect to flaws in steam generator tubing. The problem is complicated because different countries take different approaches to the problem. The objectives in general are grouped in three broad areas: to avoid the unscheduled shutdown of the reactor during normal operation; to avoid tube bursts; to avoid excessive leak rates in the event of an accidental overpressure event. For each degradation mechanism in the tubes it is necessary to know answers to an array of questions, including: how well does NDT testing perform against this problem; how rapidly does such degradation develop; how well is this degradation mechanism understood. Based on the above information it is then possible to come up with a policy to look at flaw acceptance. Part of this criteria is a schedule for the frequency of in-service inspection and also a policy for when to plug flawed tubes. The author goes into a broad discussion of each of these points in his paper.

  3. Public acceptance of nuclear power in Taiwan

    It is necessary to reach the public acceptance for nuclear power development program. During the process of the application for the approval from the government to implement the Fourth Nuclear Power Plant program in Taiwan, we initialized a series of communication program in the last two years and are expecting to convince the public that to develops nuclear power is essential to the country from a viewpoint of energy diversified. The basic strategies of the communication program not only emphasized the new nuclear power project, but also for the long term public acceptance on nuclear power. The strategies include: (1) Preview and implement the promotion program for the performance of the existing nuclear power plants. (2) Designate and communicate with the major communication target groups: elected delegates, journalists, local residents, scholars and experts. (3) Edit and incorporate the basic nuclear knowledge into the preliminary school educational materials. (4) Subsidize the adjacent communities of nuclear power plants for the public well-being construction. In order to implement the mentioned strategies, Taipower has reorganized the public service department and the existing nuclear power plants, setup the nuclear exhibition center, conducted fullscale emergency drill biannually for each of nuclear power plant, and prepared the seminars for the teacher

  4. Acceptability of Contraceptives in Young Couples

    楼超华; 郭友宁

    1997-01-01

    Based on the data from the survey of 7826 young couples in two districts of Shanghai, we analyzed the acceptability of oral pill, IUD, condom and injection in women. The results showed that 63.4% of subjects were unwilling to use pill and 5.7% were unwilling to use injection, mainly for the reasons that the pill was “harmful tohealth” and the “cause of obesity”; 8.7% of subjects were unwilling to use IUD, mainly for the reasons of the “cause of menorrhagia or spotting” and“being easy to fail”; 6.2% of subjects were unwilling to use condom, mainly for the reasons of “interference with intercourse” and “inconvenience in use”. The most important source of getting the information was “heard from person's talking”.Simple variate and Logistic analysis showed that women's age at marriage, education, occupation, contraceptive knowledge score, history of using the contraceptives,some women's traits etc. affected the acceptability of the contraceptives. The study implies that it is necessary to strengthen provision of contraceptive knowledge for young couples and to make them have correct understanding of the side effects of contraceptives for improving family planning program.

  5. Assesing the acceptance of the project

    Clara Fernández-Shaw Toda

    2009-11-01

    Full Text Available OBJECTIVE: Evaluate the acceptability of the project "Adding colour to your unit dose." MATERIAL AND METHODS: It sets in motion a project to reduce errors in the administration of pasting labels with different shapes and colours on blister medicines. It includes the name of the product, the active ingredient, dosage, lot and expiration date. Also included for internal quality control are the initials of the person who made the label and the date and time at which it was made. The nurse monitors the consistency between the data and the label of the drug. After 4 years the external acceptance of the project is analyzed through a satisfaction survey administered to the professionals involved in prescribing and dispensing medicines. RESULTS: 100% of the physicians believed that the project is adequate. Assistants and nurses expressed a dissatisfaction of 58.3% and 26.66% respectively due to the difficulty of extracting the specialty. CONCLUSIONS: - There is agreement on the need to clearly differentiate drugs, which traditional packaging does not resolve while “Adding colour to your unit dose" does. - The pharmacy prepared labels with a flap to facilitate their opening, thereby improving the current system.

  6. Nuclear power development and public acceptance

    The public acceptance aimed at realizing the development of nuclear energy of 49,000,000 kW in 1985 in accordance with the basic policy of the combined energy measures in Japan determined by the cabinet meeting in December 1975 is discussed, and the three principles, namely democracy, independence and disclosures under Article 2 of the Atomic Energy Act, are reviewed. Stronger friction may occur owing to the following characteristics of nuclear power development: it is comprehensive but apt to be sectional, too large scale, very long term, military secrecy, and international relations. In addition, there are the sensibility to radioactivity and illogical refusal in Japan. As to the democracy, the participation of the people to the development of nuclear power has not so far been considered. For example, the holding of public hearings has not been legal obligation, but only a by-law of Atomic Energy Commission. As to the independence, serious troubles have been experienced because of the complete dependence of U.S. techniques. As to the disclosure, the opening to the public of the commercial secrecy attached to the application for the approval of reactor installations is apt to be much disputed. In conclusion, it is believed that there is no other way than the exertion of straight effort under the three principles, so that the formation of public acceptance will be accomplished. (Iwakiri, K.)

  7. Acceptance testing and QA in interventional cardiology

    Interventional radiology has seen rapid growth in the field of cardiology over the past decade. This growth has been facilitated by advances in imaging technology and the development of increasingly sophisticated dedicated radiological equipment. Interventional cardiology (IC) procedures are complex and may involve prolonged irradiations, which may subject patients and operators to higher levels of risk than those, which normally prevail. Currently interventional cardiology contributes over 10% to annual collective dose in the UK in spite of contributing to a total annual frequency of 0.68%. The EU Medical Exposures Directive, 97/43/Euratom, identifies interventional radiology as an area of special concern. Acceptance testing and routine quality assurance (QA) programmes are particularly important in the field of interventional cardiology given the above. The requirements for acceptance testing and QA are underpinned in the EU Medical Exposures Directive and consequent national legislation. A QA survey of 16 interventional cardiology systems in Ireland was carried out by the Department of Medical Physics and Bioengineering, St. James's Hospital, Dublin. This is the continuation of a recently published survey and represents over half of the interventional cardiology systems in Ireland including new technology digital flat panel systems and dedicated mobile equipment in trailors. Acceptance testing and QA protocols were developed and reviewed in accordance with current international and national guidelines, standards and literature. Testing included assessing the performance of the X ray tube and generator, the automatic exposure control (AEC) device in fluoroscopy and digital acquisition modes and a subjective assessment of image quality using the Leeds test objects. Radiation shielding calculations and measurements were performed to determine the structural shielding required by new installations and electrical, mechanical and general radiation safety was also

  8. Public Acceptance A Threefold Application of Social Psychology to Enhance People's Acceptance of Nuclear Energy

    It provides three aspects of application of social psychological theories and methodology. The first aspect deals with the study of people's attitudes toward nuclear energy; the second with the development of programs in order to promote people's acceptance of nuclear energy; and the third with the measurement of the effectiveness of the programs that have been implemented to promote the public acceptance. In Korea, very few studies have been conducted to explain people's attitudes toward nuclear power. The existing data tell US that the public attitudes are generally favorable. Would they stay that way? The outlook is not very bright, considering the rapid growth of the environmental movement in Korea

  9. DNA extraction by zinc.

    Kejnovský, E; Kypr, J

    1997-01-01

    A fast, very simple and efficient method of DNA extraction is described which takes advantage of DNA sedimentation induced by millimolar concentrations of ZnCl2. The zinc-induced sedimentation is furthermore strongly promoted by submillimolar phosphate anion concentrations. Within 90% of DNA irrespective of whether a plasmid DNA or short oligonucleotides are the extracted material. The method works with plasmid DNA and oligonucleotide concentrations as low as 100 ng/ml and 10 microg/ml, respe...

  10. Wedging out DNA damage

    Schärer, Orlando D.; Campbell, Arthur J

    2009-01-01

    The DNA-repair machinery is faced with the significant challenge of differentiating DNA lesions from unmodified DNA. Two recent publications, one in this issue of Nature Structural & Molecular Biology, uncover a new way of recognizing minimally distorting DNA lesions: insertion of a 3- or 4-amino-acid wedge into DNA to extrude the lesion into a shallow binding pocket that can accommodate various damaged bases.

  11. Acceptance of dying: a discourse analysis of palliative care literature.

    Zimmermann, Camilla

    2012-07-01

    The subject of death denial in the West has been examined extensively in the sociological literature. However, there has not been a similar examination of its "opposite", the acceptance of death. In this study, I use the qualitative method of discourse analysis to examine the use of the term "acceptance" of dying in the palliative care literature from 1970 to 2001. A Medline search was performed by combining the text words "accept or acceptance" with the subject headings "terminal care or palliative care or hospice care", and restricting the search to English language articles in clinical journals discussing acceptance of death in adults. The 40 articles were coded and analysed using a critical discourse analysis method. This paper focuses on the theme of acceptance as integral to palliative care, which had subthemes of acceptance as a goal of care, personal acceptance of healthcare workers, and acceptance as a facilitator of care. For patients and families, death acceptance is a goal that they can be helped to attain; for palliative care staff, acceptance of dying is a personal quality that is a precondition for effective practice. Acceptance not only facilitates the dying process for the patient and family, but also renders care easier. The analysis investigates the intertextuality of these themes with each other and with previous texts. From a Foucauldian perspective, I suggest that the discourse on acceptance of dying represents a productive power, which disciplines patients through apparent psychological and spiritual gratification, and encourages participation in a certain way to die. PMID:22513246

  12. What makes nuclear energy (not) acceptable?

    Higher knowledge has long been hypothesized as leading to better acceptance of nuclear energy, but in the last years other factors such as risk perception and trust in nuclear risk governance were also recognized as key elements. While stakeholder involvement is now fully recognized as a key element for nuclear energy acceptance, there are still questions about the impact of higher knowledge. This paper investigates the relation between knowledge about the nuclear domain, risk perception of nuclear risks, confidence in the management of nuclear technologies, on the one hand, and the attitude towards nuclear energy and opinion about nuclear energy, on the other hand. It also studies the factors that are pleading in favour or against nuclear energy and their relation with the forementioned variables. The study is based on empirical data from a large scale opinion survey in Belgium between 25/05/2011 and 24/06/2011, i.e. the third month after the accident in Fukushima. The sample consisted of 1020 respondents and is representative for the Belgian adult population (18+) with respect to gender, age, region, province, habitat and social class. Our results show that confidence in the safe management of nuclear technologies as well as the perceived strength of the arguments pro/against nuclear are driving factors for people's attitude towards nuclear energy. Higher confidence and stronger adherence to the arguments in favour of nuclear energy lead to higher acceptance. The correlation between knowledge and attitude/opinion towards nuclear energy is statistically significant, but rather low, showing only a weak effect of knowledge on attitudes or opinions about nuclear energy. A weak effect is also observed for risk perception of nuclear risks, lower risk perception leading to a somewhat more positive attitude/opinion about nuclear energy. In the study we also highlight that the main factors seen as pleading in favour or against nuclear energy are the same, both for people

  13. Immunostimulatory DNA-based vaccines induce cytotoxic lymphocyte activity by a T-helper cell-independent mechanism.

    Cho, H J; Takabayashi, K; Cheng, P M; Nguyen, M D; Corr, M; Tuck, S; Raz, E

    2000-05-01

    Immunostimulatory DNA sequences (ISS) contain unmethylated CpG dinucleotides within a defined motif. Immunization with ISS-based vaccines has been shown to induce high antigen-specific cytotoxic lymphocyte (CTL) activity and a Th1-biased immune response. We have developed a novel ISS-based vaccine composed of ovalbumin (OVA) chemically conjugated to ISS-oligodeoxynucleotide (ODN). Protein-ISS conjugate (PIC) is more potent in priming CTL activity and Th1-biased immunity than other ISS-based vaccines. Cytotoxic lymphocyte activation by ISS-ODN-based vaccines is preserved in both CD4-/- and MHC class II-/- gene-deficient animals. Furthermore, PIC provides protection against a lethal burden of OVA-expressing tumor cells in a CD8+ cell-dependent manner. These results demonstrate that PIC acts through two unique mechanisms: T-helper-independent activation of CTL and facilitation of exogenous antigen presentation on MHC class I. This technology may have clinical applications in cancer therapy and in stimulating host defense in AIDS and chronic immunosuppression. PMID:10802617

  14. Purification of BsuE methylase to investigate the role of DNA methylation in rat growth hormone gene expression

    DNA methylation at specific cytosine bases is one mechanism believed to be involved in determining the tissue specific gene expression. We have directly tested the effect of site specific methylation at the CGCG site on rat growth hormone (rGH) promoter activity. 1.5 kilobasepairs of rGH promoter sequences were inserted in front of two bacterial indicator genes, Neo and CAT. A CGCG specific methylase was isolated from Bacillus subtilis strain ISE15 by three column chromatography steps: phosphocellulose, heparin-sepharose and DEAE-Sepharose. Its molecular weight was 41,000 by gel filtration. This enzyme was used to methylate the CGCG sequence in GH1-Neo and GH1-CAT. Methylated and unmethylated fusion genes were transferred into GH3 rat pituitary tissue culture cells by calcium-phosphate coprecipitation or electroporation. Cells transfected with GH1-Neo were harvested after 2 days, replated at 5 x 105 cells/100 mm dish in selective media, and counted after 2 weeks. Cells were harvested 36 hours after transfection with GH1-CAT and acetylation of 14C-chloramphenicol measured in whole cell extracts

  15. Acceptance Test Report for 241-U compressed air system

    This Acceptance Test Report (ATR) documents the results of acceptance testing of a newly upgraded compressed air system at 241-U Farm. The system was installed and the test successfully performed under work package 2W-92-01027

  16. Social Acceptance of a Teleoperated Android

    Ryuji, Yamazaki; Nishio, Shuichi; Ishiguro, Hiroshi;

    2012-01-01

    to its huggable minimalistic human design were cross-culturally shared in Denmark and Japan. Contrary to the negative reactions by non-users in media reports, our result suggests that teleoperated androids can be accepted by the elderly as a kind of universal design medium for social inclusion.......We explored the potential of teleoperated android robots, which are embodied telecommunication media with humanlike appearances, and how they affect people in the real world when they are employed to express a telepresence and a sense of ‘being there’. In Denmark, our exploratory study focused...... on the social aspects of Telenoid, a teleoperated android, which might facilitate communication between senior citizens and Telenoid’s operator. After applying it to the elderly in their homes, we found that the elderly assumed positive attitudes toward Telenoid, and their positivity and strong attachment...

  17. Stakeholder acceptance analysis ResonantSonic drilling

    This report presents evaluations, recommendations, and requirements concerning ResonantSonic Drilling (Sonic Drilling), derived from a three-year program of stakeholder involvement. Sonic Drilling is an innovative method to reach contamination in soil and groundwater. The resonant sonic drill rig uses counter-rotating weights to generate energy, which causes the drill pipe to vibrate elastically along its entire length. In the resonant condition, forces of up to 200,000 pounds are transmitted to the drill bit face to create a cutting action. The resonant energy causes subsurface materials to move back into the adjacent formation, permitting the drill pipe to advance. This report is for technology developers and those responsible for making decisions about the use of technology to remediate contamination by volatile organic compounds. Stakeholders' perspectives help those responsible for technology deployment to make good decisions concerning the acceptability and applicability of sonic drilling to the remediation problems they face

  18. KNOWLEDGE AND ACCEPTANCE OF RFID TRACEABILITY SOLUTIONS

    Zoltan KATO

    2014-06-01

    Full Text Available In recent years the food market has become highly dynamic. Food crises in the early 90's, or those of the recent years, such as avian flu pandemic, swine flu, the cucumber or horse meat crises all had a negative impact on the Romanian food industry. Food deficiencies have led not only to contamination but also to the illness or death of some consumers. The products traceability is a prerequisite for success in the food industry. As a modern solution, RFID technology can be useful for traceability applications made with rewritable passive RFID tags. This paper aims to examine how RFID technology can be useful for traceability applications and will submit a diligent search regarding the degree of knowledge of RFID technology and the acceptance of an empirical model by the employees of Romanian companies.

  19. Stakeholder acceptance analysis: Tunable hybrid plasma

    This report resents evaluations, recommendations, and requirements concerning Tunable Hybrid Plasma (THP) derived from a three-year program of stake holder involvement. THP destroys volatile organic compounds by directing a moderate energy electron beam into a flow of air containing organic contaminants. This report is for technology developers and for those responsible for making decisions about the use of technology to remediate contamination by volatile organic compounds. Stakeholders' perspectives help those responsible for technology deployment make good decisions concerning the acceptability and applicability of THP to the remediation problems the face. In addition, this report presents data requirements for the technology's field demonstration defined by stakeholders associated with the Hanford site in Washington State, as well as detailed comments on THP from stakeholders from four other sites throughout the western United States

  20. Nuclear energy: beliefs, values and acceptability

    The last decade has seen a dramatic increase in public concern about nuclear energy. As a consequence, it has become recognised that the future of nuclear energy will not only depend on technical and economic factors, but that public acceptability of this technology will play a crucial role in the long-term future of nuclear energy. Research has shown a considerable divergence in public and expert assessment of the risks associated with nuclear energy. Qualitative aspects of risks play a dominant role in the public's perception of risks, and it seems necessary for experts to recognise this in order to improve relations with the general public. It is also clear, however, that differences in the perception of risks do not embrace all the relevant aspects of the public's assessment of nuclear energy. Public reaction is also related to more general beliefs and values, and the issue of nuclear energy is embedded in a much wider moral and political domain. (author)

  1. The NA49 large acceptance hadron detector

    The NA49 detector is a wide acceptance spectrometer for the study of hadron production in p+p, p+A, and A+A collisions at the CERN SPS. The main components are 4 large-volume TPCs for tracking and particle identification via dE/dx. TOF scintillator arrays complement particle identification. Calorimeters for transverse energy determination and triggering, a detector for centrality selection in p+A collisions, and beam definition detectors complete the set-up. A description of all detector components is given with emphasis on new technical realizations. Performance and operational experience are discussed in particular with respect to the high track density environment of central Pb+Pb collisions

  2. Effective communications bring greater public acceptance

    In 1986, GPU Nuclear Corporation announced a plan to evaporate into the atmosphere 2.3 million gal of water remaining from the Three Mile Island Unit 2 (TMI-2) accident. The water would be processed to remove most of the radioactivity, but still remaining were >1,000 Ci of tritium to be released to the atmosphere during the evaporation process. It was expected that, following regulatory approvals, it would take >2 yr to complete the process. Fed by well-established antinuclear groups, public concern about evaporating the TMI-2-accident-generated water ran high among residents living near the plant. In the years since the TMI-2 accident, GPU Nuclear had developed a highly effective communications program in the communities surrounding TMI. This ongoing program provided a solid foundation on which to create and implement a risk communications approach to community understanding and acceptance of the evaporation process

  3. The CEBAF Large Acceptance Spectrometer (CLAS)

    Mecking, B

    2003-01-01

    The CEBAF Large Acceptance Spectrometer (CLAS) is used to study photo- and electro-induced nuclear and hadronic reactions by providing efficient detection of neutral and charged particles over a good fraction of the full solid angle. A collaboration of about thirty institutions has designed, assembled, and commissioned CLAS in Hall B at the Thomas Jefferson National Accelerator Facility. The CLAS detector is based on a novel six-coil toroidal magnet which provides a largely azimuthal field distribution. Trajectory reconstruction using drift chambers results in a momentum resolution of 0.5% at forward angles. Cerenkov counters, time-of-flight scintillators, and electromagnetic calorimeters provide good particle identification. Fast triggering and high data acquisition rates allow operation at a luminosity of 10 sup 3 sup 4 nucleon cm sup - sup 2 s sup - sup 1. These capabilities are being used in a broad experimental program to study the structure and interactions of mesons, nucleons, and nuclei using polarize...

  4. Public acceptance of nuclear power in Japan

    Japan has a fragile energy supply structure, with 84% of its energy depending on import; for example, 99.6% of the oil comes from overseas, which makes Japan's economic base rather vulnerable. In order to ensure constant energy supply, it is indispensable to diversify the energy sources and to create indigenous energy. In view of this, nuclear energy is considered to be the main alternative to crude oil because it has several advantages over other energy sources, such as stable supply of uranium and the fact that it is compatible with efforts to find solutions to global environmental problems. However, since the general public is not familiar with nuclear technology, it is difficult to get the understanding and co-operation of people. In view of this, public relations activities providing information on the need and safety of nuclear power generation have been performed. As a result, in recent years, about 70% of the people came to recognize the need for nuclear power generation. Although people's recognition of this need has increased substantially, it is still difficult for them to accept the construction of nuclear facilities, because of their anxiety regarding the safety of such plants and the lack of information by the government and electric utilities. This makes the acquisition of new sites for nuclear power plants difficult, so that the time required for developing such plants becomes longer. In order to eliminate people's anxieties, both the government and electric utilities should provide accurate information, at the proper time and using a method that makes it easy for the people to understand the problems involved. It is also important for the government and the electric utilities to listen carefully to the opinions and questions of people and to increase friendly communications with them. The government, electric utilities and constructors of nuclear facilities have to co-operate in order to improve the measures taken to gain public acceptance of

  5. Visually Impaired Children's Acceptances on Assistive Courseware

    Nurulnadwan Aziz

    2011-01-01

    Full Text Available Problem statement: The government of Malaysia is considering serious efforts in promoting the usage of computers among disabled people. Currently, the availability of computer applications especially related to creative contents for disabled people in Malaysia is infancy and very expensive. Research and expertise in the area of Assistive Technology (AT in Malaysia is still uncommon and not extensively utilized. Therefore research involving special communities such as disabled people on the usage of electronic applications should be focused because records show that their population populations are increasing. Hence, this research will be tailored for Visually-Impaired (VI learners. In Malaysia, visual impairment is one of the highest registered disabilities. Approach: This study presents an ongoing project that determines whether the VI children can accept the Assistive Courseware (AC as one of the effective tools in their learning process. Specification identification, AC development and testing and evaluation are three activities involved in this study. In addition, data have been collected through qualitative approaches. Results: The results highlight four important elements for incorporated in an AC for VI children. Through the testing and evaluation, it was found that the VI children accept the AC due to interesting usage of audio, graphic, text and easy navigation method. Conclusion: In a nutshell, the positive responses prove that the AC not only exposes the VI children to the world of technology but also educates them in terms of interpersonal skills, intrapersonal skills and motivation. The recommended future work is to produce a much comprehensive AC which focuses on interactivity, various multimedia elements, multiple intelligence elements and multiple stages of exercise in granting value-added AC not only for VI children in Malaysia but also to other world VI communities in the world.

  6. Public acceptance of radioactive waste transportation systems

    As the thoughts of the country concentrate on the problems of transportation of waste through high traffic urban areas, the problem of how to deal directly and honestly with the public takes on greater significance in the nuclear industry. Non-technical aspects of the methods of transportation, especially by railroad and highway, enter into the total scheme of moving radioactive waste from both weapon and nuclear power plant sources to final processing and disposal. Factors such as shape, color, size, familiarity, and industrial designing are necessary ingredients that take on equal or more significance that the designing of containers to survive the hypothetical accident conditions of the present, or even of the future. Protective Packaging, Inc. has been a leader in the presentation of containers to the private and public sector of the nuclear industry. The products have undergone very open testing, in public, with both invited and uninvited witnesses. In those experiences, dating back to 1969, the problems of public acceptance will be related between the technical problems and the associated social and political problems that relate to container acceptance by the public in today's world. Proven experience data, relative to the safety of the present day systems will be discussed, as well as methods of improving the image in the future. Review will also be given to the effort by industry to discuss the proven record with parties outside the nuclear industry, i.e., individuals and pressure groups that are diametrically opposed to review the facts relative to safety as opposed to other, but more traditional industries

  7. Acceptance of food irradiation in western markets

    This paper reviews the status and acceptance of food irradiation worldwide, focusing on Europe and the United States. Today no less than 38 countries including the USA and 14 European countries, have approved the irradiation of food. Across Europe there is a very wide variation, with a variety of foods being irradiated and eaten in Belgium and France but a total ban on food irradiation in Germany. Progress towards a directive harmonising the position across all countries in the European Union is slow. In the USA there is a growing awareness of the advantages of using food irradiation to combat the increasing risk of the food-borne diseases, and media coverage and consumer attitudes are considerably more favourable than previously. The use of irradiation instead of pesticides for spice treatment is gaining acceptance within the North American spice industry and the NA meat industry is recognising the potential of food irradiation as one way of meeting its obligations under the new HACCP regulations. Food irradiation is also being seriously considered as an alternative to the use of methyl bromide for quarantine treatment of fruit and vegetables. The establishment of the World Trade Organisation in 1995 to enforce various agreements concluded during the GATT Uruguay Round is expected to impact trade liberalisation. In particular the agreements pertaining to the Application of Sanitary and Phytosanitary Measures (SPS) and on Technical Barriers to Trade (TBT) have particular reference to track in irradiated food. In this respect, it is particularly important for potential training partners (food producing countries) to ensure that they have domestic approvals in place for any irradiated foods they provide to western countries. (author). countries. (author)

  8. 3DNA: A Tool for DNA Sculpting

    Gupta, Shikhar Kumar; Joshi, Foram; Limbachiya, Dixita; Gupta, Manish K.

    2014-01-01

    DNA self-assembly is a robust and programmable approach for building structures at nanoscale. Researchers around the world have proposed and implemented different techniques to build two dimensional and three dimensional nano structures. One such technique involves the implementation of DNA Bricks proposed by Ke et al., 2012 to create complex three-dimensional (3D) structures. Modeling these DNA nano structures can prove to be a cumbersome and tedious task. Exploiting the programmability of b...

  9. Acceptance tests of a new gamma camera

    For best patient service, a QA programme is needed to produce quantitative/qualitative data and keep records of the results and equipment faults. Gamma cameras must be checked against the manufacturer's specifications.The service manual is usually useful to achieve this goal. Acceptance tests are very important not only to accept a new gamma camera system for routine clinical use but also to have a role in a reference for future measurements. In this study, acceptance tests were performed for a new gamma camera in our department. It is a General Electric MG system with two detectors, two collimators. They are low energy general purpose (LEGP) and medium energy general purpose (MEGP). All intrinsic calibrations and corrections were done by the service engineer at installation (PM tune, dynamic correction, energy calibration, geometric calibration, energy correction, linearity correction and second order corrections).After installation, calibrations and corrections, a close physical inspection of the mechanical and electrical safety aspects of the cameras were done by the responsible physicist of the department. The planar system is based on measurement of system uniformity, resolution/linearity and multiple window spatial registration. All test procedures were performed according to NEMA procedures developed by the manufacturer. Intrinsic uniformity: NEMA uniformity was done first by using service manual and then other isotope uniformities were acquired with 99mTc, 131I, 201Tl and 67Ga. They were evaluated qualitatively and quantitatively, but non-uniformities were observed, especially for detector II, The service engineers repeated all tests and made necessary corrections. We repeated all the intrinsic uniformity tests. 99mTc intrinsic images were also performed at 'no correction', 'no energy correction', 'no linearity correction', 'all correction' and '±10% off peak', and compared. Extrinsic uniformity: At the beginning, collimators were checked for defects

  10. SPECT: How much acceptance testing is reasonable?

    Full text: Whereas in radiology national standards for acceptance testing of medical instrumentation are available, in nuclear medicine this is not the case. This resulted in some kind of uncertainty, so that the Austrian Societies for Nuclear Medicine and for Medical Physics decided to establish a task group for developing guidelines for acceptance testing. One important item that has to be kept in mind is the fact that acceptance testing of radiological equipment has to assure the observation of prescribed limits of radiation. Otherwise there would arise the risk of increased radiation exposure of the patient. On the other hand, the aim of acceptance testing of gamma cameras is only to assure that the specifications are observed. There are no prescribed limits and there is no risk for the patient. There are two standards for defining specifications of gamma cameras: the NEMA standards and the IEC standards. Most of the manufacturers determine the specifications of their gamma cameras according to the NEMA standards, so if we want to check the specifications in the field, we have to use the same methods as the manufacturer, that is the NEMA standards. The questions are: Should we check all parameters defined in the NEMA standards? That would not be possible in the field. Which parameters should we select? In which extent and how precisely should we check? In any case, there must be a reasonable balance between time and financial effort and technical information obtained. Facing these problems our task group decided to propose the following procedure: First, we apply all tests which are called 'primary tests' in the NEMA standards: 1. Intrinsic spatial resolution 2. Intrinsic energy resolution 3. Intrinsic flood field uniformity 4. System spatial resolution without scatter 5. System alignment 6. SPECT reconstructed spatial resolution without scatter 7. Whole body system spatial resolution without scatter. All these tests are basically performed according to the

  11. Acceptance threshold theory can explain occurrence of homosexual behaviour

    Engel, Katharina C.; Männer, Lisa; Ayasse, Manfred; Steiger, Sandra

    2015-01-01

    Same-sex sexual behaviour (SSB) has been documented in a wide range of animals, but its evolutionary causes are not well understood. Here, we investigated SSB in the light of Reeve's acceptance threshold theory. When recognition is not error-proof, the acceptance threshold used by males to recognize potential mating partners should be flexibly adjusted to maximize the fitness pay-off between the costs of erroneously accepting males and the benefits of accepting females. By manipulating male b...

  12. Dark chocolate acceptability: influence of cocoa origin and processing conditions

    Torres Moreno, Míriam; Tarrega, Amparo; Costell Ibáñez, Elvira; Blanch i Colat, Consol

    2011-01-01

    BACKGROUND: Chocolate properties can vary depending on cocoa origin, composition and manufacturing procedure, which affect consumer acceptability. The aim of this work was to study the effect of two cocoa origins (Ghana and Ecuador) and two processing conditions (roasting time and conching time) on dark chocolate acceptability. RESULTS: Overall acceptability and acceptability for different attributes (colour, flavour, odour and texture) were evaluated by 95 consumers. Differences ...

  13. Blog acceptance model: An empirical study on exploring users’ acceptance and continual usage of blogs

    ZHAO; Yuxiang; ZHU; Qinghua

    2009-01-01

    Blogs have permeated into our daily lives at a fast speed,and various kinds of blog spaces have attracted our attention.However,little effort has been made on studying the users’motivation to participate in blog activities.This paper aims to construct a theoretical model about the blog adoption based on technology acceptance model(TAM theory),social capital theory and social exchange theory,and put forward 18 related hypotheses.Then the survey method is adopted to analyze the data from 208 questionnaires using the SPSS and LISREL tools,and to examine the theoretical model and hypotheses.Finally,the paper makes a discussion from five aspects due to the results of data analysis,including individual driving factors,group driving factors,community driving factors,technology acceptance factors and moderating variables.The results show that curiosity/enjoyment,user’s experience,social interaction and social identification will greatly affect users’motivation to accept a blog;meanwhile,perceived ease of use,exchange cost and trust will partially influence users’intention to participate in blog activities.The results also suggest that age and education degrees have significant moderating effects on users’acceptance and updating of blogs.

  14. Analytical comparison of Technology Acceptance Model and theory of reasoned action about mobile advertising acceptance

    Mohamad Tabatabaei Nasab

    2014-03-01

    Full Text Available With the emergence of high speed wireless network technologies and the increasing market penetration of cell phones the global advertising industry’s interest in using this medium as a means of marketing communication is rising. However, in spite of the increasing number of companies investing in mobile marketing campaigns, there is, as yet, little academic research on this topic and the nature and implications of this channel are not yet understood fully.Mobile advertising refers to advertisements delivered through a mobile device in form of text message. Current research is considered as applied objectively and its methodology is descriptive-analytical. Research data is gathered through distribution of questionnaire among a sample of 578 Yazd university students. To study users' attitude and intention, Technology Acceptance Model and theory of reasoned action are applied for accepting mobile advertising. Also SPSS16 and Lisrel 850 softwares are used for data analysis and research models are compared according to goodness of fit indices. On the basis of Lisrel structural equation results, both aforementioned models of mobile advertising acceptance were well-fitted and it is proved that the theory of reasoned action was fitted better than Technology Acceptance Model.

  15. Acceptance- versus Change-Based Pain Management: The Role of Psychological Acceptance

    Blacker, Kara J.; Herbert, James D.; Forman, Evan M.; Kounios, John

    2012-01-01

    This study compared two theoretically opposed strategies for acute pain management: an acceptance-based and a change-based approach. These two strategies were compared in a within-subjects design using the cold pressor test as an acute pain induction method. Participants completed a baseline pain tolerance assessment followed by one of the two…

  16. User Acceptance of YouTube for Procedural Learning: An Extension of the Technology Acceptance Model

    Lee, Doo Young; Lehto, Mark R.

    2013-01-01

    The present study was framed using the Technology Acceptance Model (TAM) to identify determinants affecting behavioral intention to use YouTube. Most importantly, this research emphasizes the motives for using YouTube, which is notable given its extrinsic task goal of being used for procedural learning tasks. Our conceptual framework included two…

  17. Examining Engineering & Technology Students' Acceptance of Network Virtualization Technology Using the Technology Acceptance Model

    Yousif, Wael K.

    2010-01-01

    This causal and correlational study was designed to extend the Technology Acceptance Model (TAM) and to test its applicability to Valencia Community College (VCC) Engineering and Technology students as the target user group when investigating the factors influencing their decision to adopt and to utilize VMware as the target technology. In…

  18. DNA Polymerase-Catalyzed DNA Network Growth

    Keller, Sascha; Wang, Jie; Chandra, Madhaviah; Berger, Rüdiger; Marx, Andreas

    2008-01-01

    The distinct base pairing property of DNA is an advantageous phenomenon that has been exploited in the usage of DNA as scaffold for directed self-organization to form nanometer-sized objects in a desirable fashion. Herein we report the construction of three-dimensional DNA-based networks that can be generated and amplified by the DNA polymerase chain reaction (PCR). The approach is flexible allowing tuning of the meshes of the network by variation of the size of the template. Additionally, fu...

  19. Research progress of immunoadjuvant CpG DNA%CpG DNA佐剂研究进展

    孙燕

    2012-01-01

    CpG DNA containing unmethylated CpG motifs directly activates human plasmacytoid dendritic cells and B cells to induce immune response via TLR9.CpG DNA can improve function of professional antigen-presenting cells and boost vaccine-specific immune responses.Immunization with vaccine and CpG DNA maintains persistent immunity,and has systemic and mucosal immune effects significantly.Preclinical and clinical trials demonstrate that CpG DNAs are safe and effective as vaccine adjuvants and can boost the efficacy and immunogenicity of vaccines targeting infectious diseases and cancer.%CpG DNA是含有非甲基化CpG基序的寡脱氧核苷酸,通过Toll样受体9(TLR9)激活树突状细胞(DC)和B细胞产生免疫应答.CpG DNA可以促进专职抗原呈递细胞有效地呈递抗原,从而增强疫苗的特异性免疫应答.目前使用CpG DNA佐剂的疫苗免疫后能维持长期免疫,具有明显的全身和黏膜免疫效果.临床前和临床试验表明,CpGDNA佐剂安全有效,能够提高传染病和肿瘤疫苗的效能,增强疫苗的免疫原性.

  20. Exposure to Pornography and Acceptance of Rape Myths.

    Allen, Mike; And Others

    1995-01-01

    Summarizes the literature examining the association between acceptance of rape myths and exposure to pornography. States that nonexperimental methodology shows that exposure to pornography does not increase rape myth acceptance, while experimental studies show that exposure to pornography increases rape myth acceptance. Concludes that experimental…

  1. Social Acceptance of Wind: A Brief Overview (Presentation)

    Lantz, E.

    2015-01-01

    This presentation discusses concepts and trends in social acceptance of wind energy, profiles recent research findings, and discussions mitigation strategies intended to resolve wind power social acceptance challenges as informed by published research and the experiences of individuals participating in the International Energy Agencies Working Group on Social Acceptance of Wind Energy

  2. 46 CFR 164.023-9 - Samples submitted for acceptance.

    2010-10-01

    ... submitted for acceptance. Application samples. A product sample submitted for acceptance as required by... 46 Shipping 6 2010-10-01 2010-10-01 false Samples submitted for acceptance. 164.023-9 Section 164.023-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION,...

  3. 20 CFR 229.81 - Refusal to accept vocational rehabilitation.

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Refusal to accept vocational rehabilitation... Refusal to accept vocational rehabilitation. The DIB O/M is not payable for any month in which the disabled employee refuses, without good reason, to accept vocational rehabilitation services...

  4. 5 CFR 531.409 - Acceptable level of competence determinations.

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Acceptable level of competence... REGULATIONS PAY UNDER THE GENERAL SCHEDULE Within-Grade Increases § 531.409 Acceptable level of competence... delegated shall determine which employees are performing at an acceptable level of competence. (b) Basis...

  5. A Note on Risk Acceptance, Bankruptcy Avoidance and Riskiness Measures

    Chamorro Elosua, Arritokieta; Usategui Díaz de Otalora, José María

    2013-01-01

    In this work we clarify the relationships between riskiness, risk acceptance and bankruptcy avoidance. We distinguish between the restriction on the current wealth required to make a gamble acceptable to the decision maker and the restriction on the current wealth required to guarantee no bankruptcy if a gamble is accepted. We focus on the measure of riskiness proposed by Foster and Hart.

  6. DNA polymerase δ and DNA repair: DNA repair synthesis in human fibroblasts requires DNA polymerase δ

    When UV-irradiated cultured diploid human fibroblasts were permeabilized with Brij-58 then separated from soluble material by centrifugation, conservative DNA repair synthesis could be restored by a soluble factor obtained from the supernate of similarly treated HeLa cells. Monoclonal antibody to KB cell DNA polymerase α, while binding to HeLa DNA polymerase α, did not bind to the HeLa DNA polymerase δ. Moreover, at micromolar concentrations N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate (BuPdGT) and 2(p-n-butylanilino)-2'-deoxyadenosine 5'-triphosphate (BuAdATP) were potent inhibitors of DNA polymerase α, but did not inhibit the DNA polymerase δ. Neither purified DNA polymerase α nor β could promote repair DNA synthesis in the permeabilized cells. Furthermore, if monoclonal antibodies to DNA polymerase α BuPdGTP, or BuAdATP was added to the reconstituted system, there was no significant inhibition

  7. Stress-induced DNA Damage biomarkers: Applications and limitations

    Nikitaki, Zacharenia; Hellweg, Christine; Georgakilas, Alexandros; Ravanat, Jean-Luc

    2015-06-01

    A variety of environmental stresses like chemicals, UV and ionizing radiation and organism’s endogenous processes like replication stress and metabolism can lead to the generation of reactive oxygen and nitrogen species (ROS/RNS) that can attack cellular vital components like DNA, proteins and lipid membranes. Among them, much attention has been focused on DNA since DNA damages play a role in several biological disorders and aging processes. Thus, DNA damage can be used as a biomarker in a reliable and accurate way to quantify for example radiation exposure and can indicate its possible long term effects and cancer risk. Based on the type of DNA lesions detected one can hypothesize on the most probable mechanisms involved in the formation of these lesions for example in the case of UV and ionizing radiation (e.g. X- or α-, γ-rays, energetic ions, neutrons). In this review we describe the most accepted chemical pathways for DNA damage induction and the different types of DNA lesions, i.e. single, complex DNA lesions etc. that can be used as biomarkers. We critically compare DNA damage detection methods and their limitations. In addition to such DNA damage products, we suggest possible gene inductions that can be used to characterize responses to different types of stresses i.e. radiation, oxidative and replication stress, based on bioinformatic approaches and stringent meta-analysis of literature data.

  8. Public Acceptance for Geological CO2-Storage

    Schilling, F.; Ossing, F.; Würdemann, H.; Co2SINK Team

    2009-04-01

    Public acceptance is one of the fundamental prerequisites for geological CO2 storage. In highly populated areas like central Europe, especially in the vicinity of metropolitan areas like Berlin, underground operations are in the focus of the people living next to the site, the media, and politics. To gain acceptance, all these groups - the people in the neighbourhood, journalists, and authorities - need to be confident of the security of the planned storage operation as well as the long term security of storage. A very important point is to show that the technical risks of CO2 storage can be managed with the help of a proper short and long term monitoring concept, as well as appropriate mitigation technologies e.g adequate abandonment procedures for leaking wells. To better explain the possible risks examples for leakage scenarios help the public to assess and to accept the technical risks of CO2 storage. At Ketzin we tried the following approach that can be summed up on the basis: Always tell the truth! This might be self-evident but it has to be stressed that credibility is of vital importance. Suspiciousness and distrust are best friends of fear. Undefined fear seems to be the major risk in public acceptance of geological CO2-storage. Misinformation and missing communication further enhance the denial of geological CO2 storage. When we started to plan and establish the Ketzin storage site, we ensured a forward directed communication. Offensive information activities, an information centre on site, active media politics and open information about the activities taking place are basics. Some of the measures were: - information of the competent authorities through meetings (mayor, governmental authorities) - information of the local public, e.g. hearings (while also inviting local, regional and nation wide media) - we always treated the local people and press first! - organizing of bigger events to inform the public on site, e.g. start of drilling activities (open

  9. DNA fragmentation in apoptosis

    2000-01-01

    Cleavage of chromosomal DNA into oligonucleosomal size fragments is an integral part of apoptosis. Elegant biochemical work identified the DNA fragmentation factor (DFF) as a major apoptotic endonuclease for DNA fragmentation in vitro. Genetic studies in mice support the importance of DFF in DNA fragmentation and possibly in apoptosis in vivo. Recent work also suggests the existence of additional endonucleases for DNA degradation. Understanding the roles of individual endonucleases in apoptosis, and how they might coordinate to degrade DNA in different tissues during normal development and homeostasis, as well as in various diseased states, will be a major research focus in the near future.

  10. ENHANCING STAKEHOLDER ACCEPTANCE OF BIOREMEDIATION TECHNOLOGIES

    Focht, Will; Albright, Matt; Anex, Robert P., Jr., ed.

    2009-04-21

    This project inquired into the judgments and beliefs of people living near DOE reservations and facilities at Oak Ridge, Tennessee; Hanford, Washington; and Los Alamos, Tennessee about bioremediation of subsurface contamination. The purpose of the investigation was to identify strategies based on these judgments and beliefs for enhancing public support of bioremediation. Several methods were used to collect and analyze data including content analysis of transcripts of face-to-face personal interviews, factor analysis of subjective perspectives using Q methodology, and statistical analysis of results from a large-sample randomized telephone survey. Content analysis of interview transcripts identified themes about public perceptions and constructions of contamination risk, risk management, and risk managers. This analysis revealed that those who have no employment relationship at the sites and are not engaged in technical professions are most concerned about contamination risks. We also found that most interviewees are unfamiliar with subsurface contamination risks and how they can be reduced, believe they have little control over exposure, are frustrated with the lack of progress in remediation, are concerned about a lack of commitment of DOE to full remediation, and distrust site managers to act in the public interest. Concern is also expressed over frequent site management turnover, excessive secrecy, ineffective and biased communication, perceived attempts to talk the public into accepting risk, and apparent lack of concern about community welfare. In the telephone survey, we asked respondents who were aware of site contamination about their perceptions of risk from exposure to subsurface contamination. Response analysis revealed that most people believe that they are at significant risk from subsurface contamination but they acknowledge that more education is needed to calibrate risk perceptions against scientific risk assessments. Most rate their personal

  11. Public acceptance in radioactive waste management

    Radioactive waste, unavoidable by-products of economically developed societies, arises from the production of energy by nuclear fission reactors as well as from medical, research and industrial applications of radioactive materials. The main objective of radioactive waste management is the safety as well the protection of public health and the environment. The first approach for the disposal of radioactive waste was based on the traditional 'decide, announce and defend' model, focused almost exclusively on technical content. In spite of the significant technical progress that would ensure long-term safety, the rate of progress towards implementing such solutions has been slower than expected, partly attributable to an earlier technical optimism and to an underestimation of the societal and political dimensions. It is now broadly recognized that radioactive waste management involves both technical and societal dimensions which cannot be dissociated. Because of changes in society's decision-making environment and heightened public sensitivity to all matters connected with environmental protection, nuclear power, radioactivity, and especially radioactive waste, any decision regarding whether, when and how to implement waste management solutions will typically require thorough public examination and the involvement of many relevant stakeholders. The building of a long-term relationship with the local communities and the waste management facility is one of the most important contributors to sustainable radioactive waste management solutions. A new approach in now in place at international level, based on 'engage, interact and co-operate', for which both technical and societal issues are to be reconciled. That means that the involvement of all interested parties in the decision-making process is a condition for a successful and publicly acceptable implementation of such a project. A central role in the public acceptance of nuclear technologies play the management and

  12. An approach for determining the acceptable levels of nuclear risk

    The objective of this study was to develop a methodology for determining the acceptable levels of risk with respect to nuclear energy. It was concluded that the Atomic Energy Control Board should identify the interest groups that affect its choice of an acceptable level of risk, determine their expectations, and balance the expectations of the various groups such that the resulting acceptable level of risk is still acceptable to the Board. This would be done by interviewing experts on the subject of nuclear safety, developing and pretesting a public questionnaire, and surveying the public on acceptable cost-risk combinations

  13. Degrees of Truthfulness in Accepted Scientific Claims.

    Ahmed Hassan Mabrouk

    2008-12-01

    Full Text Available Normal 0 false false false EN-MY X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} Abstract: Sciences adopt different methodologies in deriving claims and establishing theories. As a result, two accepted claims or theories belonging to two different sciences may not necessarily carry the same degree of truthfulness. Examining the different methodologies of deriving claims in the sciences of ʿaqīdah (Islamic Creed, fiqh (Islamic Jurisprudence and physics, the study shows that ʿaqīdah provides a holistic understanding of the universe. Physics falls short of interpreting physical phenomena unless these phenomena are looked at through the ʿaqīdah holistic view. Left to itself, error may creep into laws of physics due to the methodology of conducting the physical experiments, misinterpreting the experimental results, or accepting invalid assumptions. As for fiqh, it is found that apart from apparent errors, fiqh views cannot be falsified. It is, therefore, useful to consider ʿaqīdah as a master science which would permit all other sciences to live in harmony.

  14. DNA damage and autophagy

    Both exogenous and endogenous agents are a threat to DNA integrity. Exogenous environmental agents such as ultraviolet (UV) and ionizing radiation, genotoxic chemicals and endogenous byproducts of metabolism including reactive oxygen species can cause alterations in DNA structure (DNA damage). Unrepaired DNA damage has been linked to a variety of human disorders including cancer and neurodegenerative disease. Thus, efficient mechanisms to detect DNA lesions, signal their presence and promote their repair have been evolved in cells. If DNA is effectively repaired, DNA damage response is inactivated and normal cell functioning resumes. In contrast, when DNA lesions cannot be removed, chronic DNA damage triggers specific cell responses such as cell death and senescence. Recently, DNA damage has been shown to induce autophagy, a cellular catabolic process that maintains a balance between synthesis, degradation, and recycling of cellular components. But the exact mechanisms by which DNA damage triggers autophagy are unclear. More importantly, the role of autophagy in the DNA damage response and cellular fate is unknown. In this review we analyze evidence that supports a role for autophagy as an integral part of the DNA damage response.

  15. Assessing acceptance sampling application in manufacturing electrical and electronic products

    B.M. Deros

    2008-12-01

    Full Text Available Purpose: This paper discusses the use of acceptance sampling technique as a practical tool for quality assuranceapplications to decide whether the lot is to be accepted or rejected.Design/methodology/approach: In Malaysia, single attribute acceptance sampling plan is widely practicedfor quality assurance purposes in manufacturing companies. Literature showed that majority of past studies onacceptance sampling had focused on the development and establishment of new methods for acceptance-samplingapplication. However, there is none that had investigated the relationship between acceptance sampling planselection and effectiveness of the selection. Therefore, in this study, the authors had analyzed the effectivenessthe acceptance sampling plan application method and its implementation problems in manufacturing electricaland electronics products. The study was conducted by using case study methodology at three manufacturingcompanies’ coded names: company A, B and C. In this paper, the authors would like to share the case studycompanies’ experienced of acceptance sampling plan selection and difficulties that they had faced during thecourse of implementing acceptance sampling in their production lines.Findings: The result from the three case study companies showed by implementing acceptance samplingthey could easily investigate and diagnose their suppliers’ product quality immediately upon their arrivalat the company premise.Practical implications: The continuous improvement and review of acceptance sampling plan is important toimprove the products quality and ensure continuous customer satisfaction.Originality/value: All the three case study companies agreed that acceptance sampling implementation hadimproved their product’s quality in the market place.

  16. On acceptance criteria for Mochovce startup physical tests

    Paper is focused on new acceptance criteria for physical startup tests of Mochovce Units 1, 2. The acceptance criteria are discussed at the stage of their development. The acceptance criteria determination is based on statistical processing of differences between the experimental and theoretical values of neutron-physical characteristics. The experimental values were taken from physical startup tests of Bohunice Unit 1, 2 and 4 and Dukovany Unit 1 - 4. The theoretical values were calculated by code BIPR-7. The acceptance criteria were established for critical boron concentration, core loading symmetry measurement, boric acid worth, temperature reactivity coefficient, 'ejected' control rod worth, pressure reactivity coefficient and power reactivity coefficient. The new acceptance criteria were applied to evaluate results of physical startup tests of Mochovce Unit 1 in June 1998. The acceptance criteria showed to be adequate. The summary results of statistical processing and the comparison of earlier, new and US acceptance criteria for physical startup tests are presented.(Authors)

  17. Pregnancy planning and acceptance among Danish pregnant women

    Rasch, V; Knudsen, L B; Wielandt, H

    2001-01-01

    OBJECTIVE: To study how living conditions influence pregnancy planning and acceptance among Danish women. METHOD: A cross-sectional questionnaire study performed among 3516 pregnant women attending Odense University Hospital, Denmark. The study population consisted of women with spontaneous...... abortion, women with ectopic pregnancies, women attending antenatal care and women with induced abortion. They were divided into four groups: women with planned and accepted pregnancies (accepting planners, n=2137), women who accepted an initially unplanned pregnancy (accepting non-planners, n=1006), women...... who rejected an initially planned pregnancy (rejecting planners, n=31), and women with unplanned and rejected pregnancies (rejecting non-planners, n=342). The association between socio-economic characteristics and pregnancy planning and acceptance was evaluated by comparing accepting non-planners with...

  18. The temporal dynamics of emotional acceptance: Experience, expression, and physiology.

    Dan-Glauser, Elise S; Gross, James J

    2015-05-01

    Emotional acceptance has begun to attract considerable attention from researchers and clinicians alike. It is not yet clear, however, what effects emotional acceptance has on early emotion response dynamics. To address this question, participants (N = 37) were shown emotional pictures and cued either to simply attend to them, or to accept or suppress their emotional responses. Continuous measures of emotion experience, expressive behavior, and autonomic responses were obtained. Results indicated that, compared to no regulation, acceptance led to more positive emotions, transiently enhanced expressivity, and lowered respiratory rate. Compared to suppression, acceptance led to more positive emotions, stronger expressivity, and smaller changes in heart rate, blood pressure, and pulse amplitude, as well as greater oxygenation. Acceptance and suppression thus have opposite effects on emotional response dynamics. Because acceptance enhances positive emotion experience and expression, this strategy may be particularly useful in facilitating social interactions. PMID:25782407

  19. The promise of acceptance as an NGO security management approach.

    Fast, Larissa; Freeman, Faith; O'Neill, Michael; Rowley, Elizabeth

    2015-04-01

    This paper explores three questions related to acceptance as a security management approach. Acceptance draws upon relationships with community members, authorities, belligerents and other stakeholders to provide consent for the presence and activities of a non-governmental organisation (NGO), thereby reducing threats from these actors. Little is documented about how NGOs gain and maintain acceptance, how they assess and monitor the presence and degree of acceptance, or how they determine whether acceptance is effective in a particular context. Based on field research conducted in April 2011 in Kenya, South Sudan and Uganda, we address each of these three issues and argue that acceptance must be actively sought as both a programme and a security management strategy. In the paper we delineate elements common to all three contexts as well as missed opportunities, which identify areas that NGOs can and should address as part of an acceptance approach. PMID:25440000

  20. DNA Open states and DNA hydratation

    It is a very well-known fact that an protonic exchange exists among natural DNA filaments and synthetic polynucleotides with the solvent (1--2). The existence of DNA open states, that is to say states for which the interior of the DNA molecule is exposed to the external environment, it has been demonstrated by means of proton-deuterium exchange (3). This work has carried out experiments measuring the dispersion of the traverse relaxation rate (4), as a pulsation rate function in a Carr-Purcell-Meiboom-Gill (CPMG) pulses sequence rate, to determine changes in the moist layer of the DNA molecule. The experiments were carried out under different experimental conditions in order to vary the probability that open states occurs, such as temperature or the exposure to electromagnetic fields. Some theoretical models were supposed to adjust the experimental results including those related to DNA non linear dynamic

  1. Heat exchanger, head and shell acceptance criteria

    Instability of postulated flaws in the head component of the heat exchanger could not produce a large break, equivalent to a DEGB in the PWS piping, due to the configuration of the head and restraint provided by the staybolts. Rather, leakage from throughwall flaws in the head would increase with flaw length with finite leakage areas that are bounded by a post-instability flaw configuration. Postulated flaws at instability in the shell of the heat exchanger or in the cooling water nozzles could produce a large break in the Cooling Water System (CWS) pressure boundary. An initial analysis of flaw stability for postulated flaws in the heat exchanger head was performed in January 1992. This present report updates that analysis and, additionally, provides acceptable flaw configurations to maintain defined structural or safety margins against flaw instability of the external pressure boundary components of the heat exchanger, namely the head, shell, and cooling water nozzles. Structural and flaw stability analyses of the heat exchanger tubes, the internal pressure boundary of the heat exchangers or interface boundary between the PWS and CWS, were previously completed in February 1992 as part of the heat exchanger restart evaluation and are not covered in this report

  2. STUDENTS' ATTITUDE AND ACCEPTANCE OF MATH STUDIES

    Sead Rešić

    2012-09-01

    Full Text Available The aim of this paper is to ascertain the type of relation elementary school students have toward mathematics classes. Through employing technical and scientific methods, a basis was constructed which can provide answers to numerous questions related to math classes, relation of students towards the subject matter, as well as to results being achieved in the subject mathematics during elementary education. The fact is that elementary school students exhibit difficulties in accepting the subject and achieve lower results in mathematics in comparison to other school subjects. Likewise, it is a fact that students upon gradating elementary school opt for high schools which have less mathematics in their curricula, and this state of affair continues when choosing higher education area. In the first theoretical part, certain fundamental concepts regarding this research topic have been clarified. The second part relates to the methodology of research of the above-mentioned problem. In the third part an analysis and interpretation of the results of conducted research has been provided.

  3. Social acceptance of carbon dioxide storage

    This article discusses public acceptance of carbon capture and storage (CCS). Responses by citizens are described in relation to responses by professionally involved actors. Interviews with members of the government, industry and environmental NGOs showed that these professional actors are interested in starting up storage projects, based on thorough evaluation processes, including discussions on multi-actor working groups. As appeared from a survey among citizens living near a potential storage site (N=103), public attitudes in general were slightly positive, but attitudes towards storage nearby were slightly negative. The general public appeared to have little knowledge about CO2-storage, and have little desire for more information. Under these circumstances, trust in the professional actors is particularly important. NGOs were found to be trusted most, and industry least by the general public. Trust in each of the three actors appeared to depend on perceived competence and intentions, which in turn were found to be related to perceived similarity of goals and thinking between trustee and trustor. Implications for communication about CCS are discussed

  4. The CEBAF large acceptance spectrometer (CLAS)

    The CEBAF large acceptance spectrometer (CLAS) is used to study photo- and electro-induced nuclear and hadronic reactions by providing efficient detection of neutral and charged particles over a good fraction of the full solid angle. A collaboration of about 30 institutions has designed, assembled, and commissioned CLAS in Hall B at the Thomas Jefferson National Accelerator Facility. The CLAS detector is based on a novel six-coil toroidal magnet which provides a largely azimuthal field distribution. Trajectory reconstruction using drift chambers results in a momentum resolution of 0.5% at forward angles. Cherenkov counters, time-of-flight scintillators, and electromagnetic calorimeters provide good particle identification. Fast triggering and high data-acquisition rates allow operation at a luminosity of 1034 nucleon cm-2 s-1. These capabilities are being used in a broad experimental program to study the structure and interactions of mesons, nucleons, and nuclei using polarized and unpolarized electron and photon beams and targets. This paper is a comprehensive and general description of the design, construction and performance of CLAS

  5. The CEBAF large acceptance spectrometer (CLAS)

    Mecking, B.A.; Adams, G.; Ahmad, S.; Anciant, E.; Anghinolfi, M.; Asavapibhop, B.; Asryan, G.; Audit, G.; Auger, T.; Avakian, H.; Ball, J.P.; Barbosa, F.J.; Barrow, S.; Battaglieri, M.; Beard, K.; Berman, B.L.; Bianchi, N.; Boiarinov, S.; Bonneau, P.; Briscoe, W.J.; Brooks, W.K.; Burkert, V.D.; Carman, D.S.; Carstens, T.; Cetina, C.; Christo, S.B.; Cole, P.L.; Coleman, A.; Connelly, J.; Cords, D.; Corvisiero, P.; Crabb, D.; Crannell, H.; Cuevas, R.C.; Degtyarenko, P.V.; Dennis, L.; DeSanctis, E.; DeVita, R.; Distelbrink, J.; Dodge, G.E.; Dodge, W.; Doolittle, G.; Doughty, D.; Dugger, M.; Duncan, W.S.; Dytman, S.; Egiyan, H.; Egiyan, K.S.; Elouadrhiri, L.; Feuerbach, R.J.; Ficenec, J.; Frolov, V.; Funsten, H.; Gilfoyle, G.P.; Giovanetti, K.L.; Golovatch, E.; Gram, J.; Guidal, M.; Gyurjyan, V.; Heddle, D.; Hemler, P.; Hersman, F.W.; Hicks, K.; Hicks, R.S.; Holtrop, M.; Hyde-Wright, C.E.; Insley, D.; Ito, M.M.; Jacobs, G.; Jenkins, D.; Joo, K.; Joyce, D.; Kashy, D.; Khandaker, M.; Kim, W.; Klein, A.; Klein, F.J.; Klusman, M.; Kossov, M.; Kramer, L.; Koubarovski, V.; Kuhn, S.E.; Lake, A.; Lawrence, D.; Longhi, A.; Lukashin, K.; Lachniet, J.; Magahiz, R.A.; Major, W.; Manak, J.J.; Marchand, C.; Martin, C.; Matthews, S.K.; McMullen, M.; McNabb, J.W.C.; Mestayer, M.D.; Minehart, R.; Mirazita, M.; Miskimen, R.; Muccifora, V.; Mueller, J.; Murphy, L.Y.; Mutchler, G.S.; Napolitano, J.; Niculescu, I.; Niczyporuk, B.B.; Nozar, M.; O' Brien, J.T.; Opper, A.K.; O' Meara, J.E.; Pasyuk, E.; Philips, S.A.; Polli, E.; Price, J.W.; Pozdniakov, S.; Qin, L.M.; Raue, B.A.; Riccardi, G.; Ricco, G.; Riggs, C.; Ripani, M.; Ritchie, B.G.; Robb, J.; Ronchetti, F.; Rossi, P.; Roudot, F.; Salgado, C.; Sapunenko, V.; Schumacher, R.A.; Serov, V.S.; Sharabian, Y.G.; Smith, E.E.S. E-mail: elton@jlab.org; Smith, L.C.; Smith, T.; Sober, D.I.; Stavinsky, A.; Stepanyan, S.; Stoler, P.; Taiuti, M.; Taylor, W.M.; Taylor, S.; Tedeschi, D.J.; Thoma, U.; Thompson, R.; Tilles, D.; Todor, L. [and others

    2003-05-11

    The CEBAF large acceptance spectrometer (CLAS) is used to study photo- and electro-induced nuclear and hadronic reactions by providing efficient detection of neutral and charged particles over a good fraction of the full solid angle. A collaboration of about 30 institutions has designed, assembled, and commissioned CLAS in Hall B at the Thomas Jefferson National Accelerator Facility. The CLAS detector is based on a novel six-coil toroidal magnet which provides a largely azimuthal field distribution. Trajectory reconstruction using drift chambers results in a momentum resolution of 0.5% at forward angles. Cherenkov counters, time-of-flight scintillators, and electromagnetic calorimeters provide good particle identification. Fast triggering and high data-acquisition rates allow operation at a luminosity of 10{sup 34} nucleon cm{sup -2} s{sup -1}. These capabilities are being used in a broad experimental program to study the structure and interactions of mesons, nucleons, and nuclei using polarized and unpolarized electron and photon beams and targets. This paper is a comprehensive and general description of the design, construction and performance of CLAS.

  6. Nuclear public acceptance and 'imaginary and reality

    Recently, anti-nuclear movement is going down, and couple reactor construction has been seemed to be accepted. Even Uranium production will be coming down, Plutonium, fast reactor and nuclear fusion reactor will coming in future. An unfortunate reactivity accident in a nuclear fuel factory in Tokai village has occurred by lack of the very essential nuclear technology which is not believable. This comes the reality has been included in an imaginary region in the brain. So picking up the reality from the imaginary region in brain is due to knowing the separation of the reality from the imaginary region by knowledge on the very essential nuclear technology. A man knowing the very essential nuclear technology can avoid this kind of accident. For the most people does not like to meet with the problems of atomic bomb and hi-radiation level waste. There two problems will be understood by knowing the reality point the imaginary area. It is easily understandable, if it is the essential nuclear technology. (author)

  7. Public acceptance of transport: international status

    In the framework of reprocessing-recycling policy chosen by the Japanese utilities in order to satisfy Japan's energy need, sea transports of spent fuel have been carried out for twenty years between Japan and Europe. All these sea shipments have been realized without any specific public interest. In 1992, the voyage of the Akatsuki Maru, carrying Japanese plutonium from COGEMA-La Hague reprocessing plant to Japan marked the beginning of interest to this kind of transports. Some concerns have been voiced by en-route States having the feeling that nuclear material is shipped 'through their backyard', and in the same time anti-nuclear organizations generated some issues on this activity. Suddenly, transportation issues which had for many years taken a back seat to other nuclear topics, have met an increasing global questioning. These circumstances went on during the first return of vitrified residues in 1995. Facing this situation, the industrial community - COGEMA, in close cooperation with its Japanese and British partners - has decided to implement a dedicated communication. It works to facilitate each on-going shipment, but also to help acceptance of coming transport campaign: for instance, one has indeed to keep in mind that one or two shipments by year of vitrified residues will take place in the next ten years. (authors)

  8. Exploring technology acceptance for mobile services

    Kenny Phan

    2011-07-01

    Full Text Available Purpose: Mobile phone industries are no longer relying on voice services. It is predicted that data services are expected to be a remediation for the declining of average revenue per user (ARPU in telecommunication market. The purpose of this paper is to find the factors that influence the acceptance/adoption of mobile services.Design/methodology/approach: This paper utilizes quantitative method. Questionnaire were distributed to collect the data. The questionnaires are designed specifically to address the factors related to mobile services. Later on, this paper uses Analytical Hierarchical Process (AHP and Cluster analysis to identify the factors that influence the adoption of mobile services.Findings: The results show that ease of use and usefulness are top two factors that influence the adoption of mobile services.Research limitations/implications: The sample for the study is relatively small. For future research, the sample should be increased. The data collection should also look into different devices and operating system of mobile phones to find if there is any significant difference in adopting mobile services.Practical implications: This framework is expected to be able to help the mobile services provider to design better mobile services that can attract users to adopt them. Originality/value: This is the follow on research on the previous study that employed different method. This study is to confirm and support the findings from previous study.

  9. Wheat products as acceptable substitutes for rice.

    Yu, B H; Kies, C

    1993-07-01

    The objective of the study was to compare the acceptability to semi-trained US American and Asian palatability panelist, of four wheat products processed to be possible replacers of rice in human diets. Products evaluated using rice as the control standard of excellence were steamed whole wheat, couscous (steamed, extracted wheat flour semolina), rosamarina (rice shaped, extracted wheat flour pasta), and bulgar (steamed, pre-cooked partly debranned, cracked wheat). Using a ten point hedonic rating scale, both groups of panelists gave rosamarina closely followed by couscous, most favorable ratings although these ratings were somewhat lower than that of the positive control, steamed polished rice. Bulgar wheat was given the lowest evaluation and was, in general, found to be an unacceptable replacement for rice by both American and Asian judges because of its dark, 'greasy' color and distinctive flavor. In their personal dietaries, judges included rice from 0.25 to 18 times per week with the Asian judges consuming rice significantly more times per week than did the American judges (10.8 +/- 4.71 vs 1.75 +/- 1.65, p < 0.01). However, rice consumption patterns, nationality, race, or sex of the judges was not demonstrated to affect scoring of the wheat products as rice replacers. PMID:8332588

  10. Grout Treatment Facility waste feed acceptance criteria

    This document establishes criteria for the acceptance of grout waste feed to provide assurance that the final grout form produced by the Grout Disposal Facility (GDF) will meet the regulatory, design, product, and process requirements. Contained in the report is an evaluation of the regulatory requirements associated with the grout disposal option along with a description of the waste currently stored on the site. An evaluation of the heat generation requirements for the waste feed stream is presented. This evaluation includes the heat resulting from the grout curing process as well as heat associated with the radiolytic decay of the radioisotopes present. Limits for individual elements as well as limits for classes of materials such as organics, sulfates, etc. are presented in Table 1-1. These values are based on regulatory, heat generation, and compositional limits to assure the integrity of the final grout products. Some compositional limits such as heavy metals will require Toxicity Characteristic Leaching Procedure (TCLP) testing to demonstrate regulatory compliance

  11. Public acceptance action plan for transportation

    The U.S. Department of Energy (DOE) Office of Civilian Radioactive Waste Management (OCRWM) is currently evaluating the Yucca Mountain site in Nevada for the potential development of a high-level radioactive waste (HLW) repository. Identification of the site for evaluation as a potential HLW disposal site sparked intense opposition by the state of Nevada. This opposition affects every aspect of dealing with the public, making treatment of transportation issues in isolation difficult. Unsafe transportation is often cited as one reason of opposition of the site. However, the excellent safety record and the well-defined transportation system (relative to the site) means transportation issues can be addressed as site evaluations progress. Transportation items raised by the public in Nevada include items that are encountered in essentially every public discussion of spent-fuel transportation and unique items as a result of negative imaging by opponents, errors in government reports, and gaps in public information efforts. A number of actions have been taken to build public acceptance of HLW transportation in Nevada. Some technical and institutional staff approaches that have been used are described

  12. Public Health Acceptance of Irradiated Food

    At its 1976 meeting the Joint FAO/IAEA/WHO Expert Committee on Food Irradiation had indicated in its Report that the irradiation of food was a process akin to other food preservation processes using physical means. On the basis of a large body of data on the microbiology, nutritional aspects and toxicology of numerous foods treated by irradiation the Committee was able to recommend unconditional acceptance for the following five foods: wheat, potato, papaya, strawberry and chicken. It also recommended provisional clearance for three other foods — rice, onion and fish — for which the results of some investigations were still outstanding. Since 1976 a considerable amount of further data have been accumulated to show that foods preserved by irradiation at doses averaging 1 Mrad (10 kGy) were wholesome and could be consumed by the public without any hazard to health. These data derived partly from extensive investigations into the radiation chemistry of irradiated food components and partly from further animal feeding studies and genetic toxicity investigations. Much of the data was generated as a result of the activities of the International Project in the Field of Food Irradiation, situated in Karlsruhe, Federal Republic of Germany.

  13. The CEBAF large acceptance spectrometer (CLAS)

    Mecking, B. A.; Adams, G.; Ahmad, S.; Anciant, E.; Anghinolfi, M.; Asavapibhop, B.; Asryan, G.; Audit, G.; Auger, T.; Avakian, H.; Ball, J. P.; Barbosa, F. J.; Barrow, S.; Battaglieri, M.; Beard, K.; Berman, B. L.; Bianchi, N.; Boiarinov, S.; Bonneau, P.; Briscoe, W. J.; Brooks, W. K.; Burkert, V. D.; Carman, D. S.; Carstens, T.; Cetina, C.; Christo, S. B.; Cole, P. L.; Coleman, A.; Connelly, J.; Cords, D.; Corvisiero, P.; Crabb, D.; Crannell, H.; Cuevas, R. C.; Degtyarenko, P. V.; Dennis, L.; DeSanctis, E.; DeVita, R.; Distelbrink, J.; Dodge, G. E.; Dodge, W.; Doolittle, G.; Doughty, D.; Dugger, M.; Duncan, W. S.; Dytman, S.; Egiyan, H.; Egiyan, K. S.; Elouadrhiri, L.; Feuerbach, R. J.; Ficenec, J.; Frolov, V.; Funsten, H.; Gilfoyle, G. P.; Giovanetti, K. L.; Golovatch, E.; Gram, J.; Guidal, M.; Gyurjyan, V.; Heddle, D.; Hemler, P.; Hersman, F. W.; Hicks, K.; Hicks, R. S.; Holtrop, M.; Hyde-Wright, C. E.; Insley, D.; Ito, M. M.; Jacobs, G.; Jenkins, D.; Joo, K.; Joyce, D.; Kashy, D.; Khandaker, M.; Kim, W.; Klein, A.; Klein, F. J.; Klusman, M.; Kossov, M.; Kramer, L.; Koubarovski, V.; Kuhn, S. E.; Lake, A.; Lawrence, D.; Longhi, A.; Lukashin, K.; Lachniet, J.; Magahiz, R. A.; Major, W.; Manak, J. J.; Marchand, C.; Martin, C.; Matthews, S. K.; McMullen, M.; McNabb, J. W. C.; Mestayer, M. D.; Minehart, R.; Mirazita, M.; Miskimen, R.; Muccifora, V.; Mueller, J.; Murphy, L. Y.; Mutchler, G. S.; Napolitano, J.; Niculescu, I.; Niczyporuk, B. B.; Nozar, M.; O'Brien, J. T.; Opper, A. K.; O'Meara, J. E.; Pasyuk, E.; Philips, S. A.; Polli, E.; Price, J. W.; Pozdniakov, S.; Qin, L. M.; Raue, B. A.; Riccardi, G.; Ricco, G.; Riggs, C.; Ripani, M.; Ritchie, B. G.; Robb, J.; Ronchetti, F.; Rossi, P.; Roudot, F.; Salgado, C.; Sapunenko, V.; Schumacher, R. A.; Serov, V. S.; Sharabian, Y. G.; Smith, E. S.; Smith, L. C.; Smith, T.; Sober, D. I.; Stavinsky, A.; Stepanyan, S.; Stoler, P.; Taiuti, M.; Taylor, W. M.; Taylor, S.; Tedeschi, D. J.; Thoma, U.; Thompson, R.; Tilles, D.; Todor, L.; Tung, T. Y.; Tuzel, W.; Vineyard, M. F.; Vlassov, A. V.; Weinstein, L. B.; Welsh, R. E.; Weygand, D. P.; Wilkin, G. R.; Witkowski, M.; Wolin, E.; Yegneswaran, A.; Yergin, P.; Yun, J.

    2003-05-01

    The CEBAF large acceptance spectrometer (CLAS) is used to study photo- and electro-induced nuclear and hadronic reactions by providing efficient detection of neutral and charged particles over a good fraction of the full solid angle. A collaboration of about 30 institutions has designed, assembled, and commissioned CLAS in Hall B at the Thomas Jefferson National Accelerator Facility. The CLAS detector is based on a novel six-coil toroidal magnet which provides a largely azimuthal field distribution. Trajectory reconstruction using drift chambers results in a momentum resolution of 0.5% at forward angles. Cherenkov counters, time-of-flight scintillators, and electromagnetic calorimeters provide good particle identification. Fast triggering and high data-acquisition rates allow operation at a luminosity of 10 34 nucleon cm -2 s-1. These capabilities are being used in a broad experimental program to study the structure and interactions of mesons, nucleons, and nuclei using polarized and unpolarized electron and photon beams and targets. This paper is a comprehensive and general description of the design, construction and performance of CLAS.

  14. HPV DNA test

    The HPV DNA test is used to check for high-risk HPV infection in women. HPV infection around the genitals is ... warts spread when you have sex. The HPV-DNA test is generally not recommended for detecting low- ...

  15. Modeling DNA Replication.

    Bennett, Joan

    1998-01-01

    Recommends the use of a model of DNA made out of Velcro to help students visualize the steps of DNA replication. Includes a materials list, construction directions, and details of the demonstration using the model parts. (DDR)

  16. Examining the Factors Affecting PDA Acceptance among Physicians: An Extended Technology Acceptance Model

    Basak, Ecem; Gumussoy, Cigdem Altin; Calisir, Fethi

    2015-01-01

    This study aims at identifying the factors affecting the intention to use personal digital assistant (PDA) technology among physicians in Turkey using an extended Technology Acceptance Model (TAM). A structural equation-modeling approach was used to identify the variables that significantly affect the intention to use PDA technology. The data were collected from 339 physicians in Turkey. Results indicated that 71% of the physicians’ intention to use PDA technology is explained by perceived us...

  17. Recombinant DNA in Medicine

    Cederbaum, Stephen D.; Fareed, George C.; Lovett, Michael A.; Shapiro, Larry J.

    1984-01-01

    Studies in bacteria and bacterial viruses have led to methods to manipulate and recombine DNA in unique and reproducible ways and to amplify these recombined molecules millions of times. Once properly identified, the recombinant DNA molecules can be used in various ways useful in medicine and human biology. There are many applications for recombinant DNA technology. Cloned complementary DNA has been used to produce various human proteins in microorganisms. Insulin and growth hormone have been...

  18. Is DNA a language?

    Tsonis, A A; Elsner, J B; Tsonis, P A

    1997-01-01

    DNA sequences usually involve local construction rules that affect different scales. As such their "dictionary" may not follow Zipf's law (a power law) which is followed in every natural language. Indeed, analysis of many DNA sequences suggests that no linguistics connections to DNA exist and that even though it has structure DNA is not a language. Computer simulations and a biological approach to this problem further support these results. PMID:9039397

  19. DNA Damage Response

    Giglia-Mari, Giuseppina; Zotter, Angelika; Vermeulen, Wim

    2011-01-01

    Structural changes to DNA severely affect its functions, such as replication and transcription, and play a major role in age-related diseases and cancer. A complicated and entangled network of DNA damage response (DDR) mechanisms, including multiple DNA repair pathways, damage tolerance processes, and cell-cycle checkpoints safeguard genomic integrity. Like transcription and replication, DDR is a chromatin-associated process that is generally tightly controlled in time and space. As DNA damag...

  20. DNA-Mediated Electrochemistry

    Gorodetsky, Alon A.; Buzzeo, Marisa C.; Barton, Jacqueline K.

    2008-01-01

    The base pair stack of DNA has been demonstrated as a medium for long-range charge transport chemistry both in solution and at DNA-modified surfaces. This chemistry is exquisitely sensitive to structural perturbations in the base pair stack as occur with lesions, single base mismatches, and protein binding. We have exploited this sensitivity for the development of reliable electrochemical assays based on DNA charge transport at self-assembled DNA monolayers. Here, we discuss the characteristi...

  1. ISS immune modulation, vaccination and the regulation of arthritis

    Ronaghy, Arash

    2007-01-01

    Bacterial DNA contains unique unmethylated CpG motifs. The cytosine-phosphate-guanisine (CpG) sequence of this motif has an unmethylated cytosine which is suppressed or methylated in mammalian DNA. Synthetic oligodeoxynucleotides containing these CpG (CpG-ODN) motifs are called Immunostimulatory DNA

  2. Immune effects of CpG-DNA and action of balancing Th1/Th2 cytokines secretion%胞嘧啶鸟嘌呤二核苷酸的免疫作用及调节Th1/Th2细胞因子的意义

    黄维琳; 刘荣玉

    2008-01-01

    非甲基化的胞嘧啶鸟嘌呤二核苷酸(CpG DNA)可激活多种免疫效应细胞,诱导并增强天然和获得性免疫应答.CpG DNA具有免疫调节作用,能诱导Th1细胞因子产生,抑制Th2细胞因子的释放,并使Th2向Th1转变,其在疫苗佐剂、支气管哮喘、肿瘤的防治中有着广阔的应用前景.%Synthetic oligodeoxynucleoties containing unmethylated CpG motifs(CpG DNA) can trigger the activation of various immune cell subsets,induce and increase innate or acquired immune responses.CpG DNA can adjust immunity.It can induce Th1 cytokines and reduce Th2 cytOkines,and change Th2 cytokines into Th1 cytokines.CpG DNA has a widespread prospect in the immune adjuvants,therapy of asthma and tumor.

  3. Replicating animal mitochondrial DNA

    Emily A. McKinney

    2013-01-01

    Full Text Available The field of mitochondrial DNA (mtDNA replication has been experiencing incredible progress in recent years, and yet little is certain about the mechanism(s used by animal cells to replicate this plasmid-like genome. The long-standing strand-displacement model of mammalian mtDNA replication (for which single-stranded DNA intermediates are a hallmark has been intensively challenged by a new set of data, which suggests that replication proceeds via coupled leading-and lagging-strand synthesis (resembling bacterial genome replication and/or via long stretches of RNA intermediates laid on the mtDNA lagging-strand (the so called RITOLS. The set of proteins required for mtDNA replication is small and includes the catalytic and accessory subunits of DNA polymerase y, the mtDNA helicase Twinkle, the mitochondrial single-stranded DNA-binding protein, and the mitochondrial RNA polymerase (which most likely functions as the mtDNA primase. Mutations in the genes coding for the first three proteins are associated with human diseases and premature aging, justifying the research interest in the genetic, biochemical and structural properties of the mtDNA replication machinery. Here we summarize these properties and discuss the current models of mtDNA replication in animal cells.

  4. DNA damage response

    G. Giglia-Mari (Giuseppina); A. Zotter (Angelika); W. Vermeulen (Wim)

    2011-01-01

    textabstractStructural changes to DNA severely affect its functions, such as replication and transcription, and play a major role in age-related diseases and cancer. A complicated and entangled network ofDNA damage response (DDR) mechanisms, including multiple DNA repair pathways, damage tolerance p

  5. DNA isolation by Chelex-100: an efficient approach to consider in leptospirosis early stages

    Angel Alberto Noda

    2014-06-01

    Full Text Available Objective: To compare the value of leptospiral DNA extraction procedures from clinical samples for the early diagnosis of leptospirosis. Methods: Three DNA extraction procedures were applied for microbiological analysis, results of QIAmp DNA mini kit (QIAGEN, Germany, CLART HPV kit (GENOMICA, Spain and Chelex-100 assay were compared concerning extraction efficiency, DNA purity and DNA suitability for amplification by specific polymerase chain reaction for pathogenic leptospires from blood, plasma and serum artificially infected. Results: The comparison of extraction methods highlighted the efficiency of Chelex-100 and QIAmp DNA mini kit. Chelex-100 achieved the isolation of the highest concentration of leptospiral DNA from the culture and the spiked samples, with acceptable purities and without inhibitors to PCR. Conclusions: Chelex-100 assay is a rapid and effective approach for DNA isolation in clinical samples having pathogenic leptospires and it could be useful in the early diagnosis of leptospirosis.

  6. DNA isolation by Chelex-100:an efficient approach to consider in leptospirosis early stages

    Angel Alberto Noda; Islay Rodríguez

    2014-01-01

    Objective:To compare the value of leptospiral DNA extraction procedures from clinical samples for the early diagnosis of leptospirosis. Methods:Three DNA extraction procedures were applied for microbiological analysis, results of QIAmp DNA mini kit (QIAGEN, Germany), CLART HPV kit (GENOMICA, Spain) and Chelex-100 assay were compared concerning extraction efficiency, DNA purity and DNA suitability for amplification by specific polymerase chain reaction for pathogenic leptospires from blood, plasma and serum artificially infected. Results: The comparison of extraction methods highlighted the efficiency of Chelex-100 and QIAmp DNA mini kit. Chelex-100 achieved the isolation of the highest concentration of leptospiral DNA from the culture and the spiked samples, with acceptable purities and without inhibitors to PCR. Conclusions: Chelex-100 assay is a rapid and effective approach for DNA isolation in clinical samples having pathogenic leptospires and it could be useful in the early diagnosis of leptospirosis.

  7. Acceptance testing and QA in dental radiology

    The HPA (UK) reported in 2002 that although the effective dose from dental examinations is low, dental imaging is the most frequent type of X ray examination undertaken (approx. 30%); a similar situation is likely to exist in Ireland. It is most likely that this will increase further in the future as the dental X ray technology moves from film to digital equipment. Dental X rays are taken more frequently with digital equipment than film based technology. The Department of Medical Physics and Bioengineering, St. James's Hospital, Dublin is particularly interested in the area of dental quality assurance (QA) as a result of a significant medical radiation safety incident in Ireland occurring with a faulty dental X ray installation. The implementation of a QA programme in Ireland has been enforced not only by the publication of the Dental Code of Practice by the Irish Regulatory Authority in the 1990s but also by the publication of the European Medical Exposures Directive. The current QA programme, which includes site visits in order to perform acceptance testing and routine QA checks on dental X ray equipment has been implemented in approximately 250 dental health centres to which the Department has provided radiation protection advisory services for approximately twenty years. In addition to the technical evaluation of the equipment, an assessment of the adequacy of the shielding in the X ray room and of the radiation protection facilities is performed. This paper identifies the findings from the dental radiology QA assessments. QA test protocols were developed in accordance with current national and internationally accepted guidelines. The tests include exposure time and kVp accuracy, radiation output consistency, filtration, irradiated field size, mechanical and electrical safety checks and measurement of tube output for specific X ray examinations to aid in the establishment of a national diagnostic reference levels, a requirement of the Medical Exposures

  8. Geothermal technology in Australia: Investigating social acceptance

    Dowd, Anne-Maree, E-mail: Anne-Maree.Dowd@csiro.au [Commonwealth Scientific and Industrial Research Organization (CSIRO), PO Box 883, Kenmore 4069 (Australia); Boughen, Naomi; Ashworth, Peta; Carr-Cornish, Simone [Commonwealth Scientific and Industrial Research Organization (CSIRO), PO Box 883, Kenmore 4069 (Australia)

    2011-10-15

    Issues of social acceptance, such as lack of awareness and negative community perceptions and reactions, can affect low emission energy technology development, despite general support observed for reducing carbon emissions and mitigating climate change. Negative community reactions and lack of understanding have affected geothermal developments, as demonstrated by the fearful community reactions and negative media experienced in response to seismic disturbances caused by 'hot rock' geothermal energy generation in Switzerland and Germany. Focusing on geothermal energy, this paper presents the results of using a participatory action research methodology to engage diverse groups within the Australian public. A key finding is that the majority of the Australian public report limited the knowledge or understanding of geothermal technology and have various concerns including water usage and seismic activity instigated by geothermal drilling. However, geothermal energy receives general support due to a common trend to champion renewable energy sources in preference to traditional forms of energy generation and controversial technologies. This paper also demonstrates the effectiveness of using an engagement process to explore public understanding of energy technologies in the context of climate change, and suggests a way forward for governments and industry to allocate resources for greatest impact when communicating about geothermal technology. - Highlights: > Majority of Australians have limited knowledge or understanding of geothermal technology. > Various concerns, including water usage and seismic activity instigated by drilling, were raised. > Geothermal energy has general support due to a common trend to champion renewable energy sources. > Methodology shows the effectiveness of an engagement process to explore public understanding. > Participants expressed intention to change behaviours, which can be a catalyst for change.

  9. Factors affecting the acceptance of nuclear energy

    Nuclear energy all over Europe faces political resistance. Politicians refer to the results of public opinion polls that show considerable resistance towards the use of nuclear power. However, responses to general questions on nuclear energy are 'soft', depending on the design of the questions, and, above all, on the situation at hand. Provided there is a need for nuclear energy, the ambivalent majority accepts this source of power. However, there is no doubt that a hard core of opponents to the use of nuclear energy under all circumstances is slowly increasing. While leadership groups prefer to explain their lack of, or only lukewarm, support for nuclear energy as being the result of public resistance, this resistance is to a large extent the result of the behaviour of key groups in opinion formation. The public has little competence in such technical issues, and also little patience in evaluating information remote from its daily concerns. The most important message the public in various countries believes it is getting from leadership groups is the impression that nuclear energy is controversial among experts. This impression is a consequence of the preferred source of information, namely television and newspapers. Surveys of journalists, politicians, scientists in general and those specializing in nuclear issues demonstrate that the farther one of the groups important in the formation of public opinion is, the more critical is its stance with regard to nuclear power. Among specialists, issues surrounding the use of nuclear power are only marginally controversial. The communications elite and cultural intelligentsia are sending the wrong messages to the general public. Thus, the first priority in rectifying this erroneous impression is to change the perceptions and attitudes of key groups involved in the formation of public opinion. 12 refs, 2 figs, 7 tabs

  10. Geothermal technology in Australia: Investigating social acceptance

    Issues of social acceptance, such as lack of awareness and negative community perceptions and reactions, can affect low emission energy technology development, despite general support observed for reducing carbon emissions and mitigating climate change. Negative community reactions and lack of understanding have affected geothermal developments, as demonstrated by the fearful community reactions and negative media experienced in response to seismic disturbances caused by 'hot rock' geothermal energy generation in Switzerland and Germany. Focusing on geothermal energy, this paper presents the results of using a participatory action research methodology to engage diverse groups within the Australian public. A key finding is that the majority of the Australian public report limited the knowledge or understanding of geothermal technology and have various concerns including water usage and seismic activity instigated by geothermal drilling. However, geothermal energy receives general support due to a common trend to champion renewable energy sources in preference to traditional forms of energy generation and controversial technologies. This paper also demonstrates the effectiveness of using an engagement process to explore public understanding of energy technologies in the context of climate change, and suggests a way forward for governments and industry to allocate resources for greatest impact when communicating about geothermal technology. - Highlights: → Majority of Australians have limited knowledge or understanding of geothermal technology. → Various concerns, including water usage and seismic activity instigated by drilling, were raised. → Geothermal energy has general support due to a common trend to champion renewable energy sources. → Methodology shows the effectiveness of an engagement process to explore public understanding. → Participants expressed intention to change behaviours, which can be a catalyst for change.

  11. Cell-specific DNA methylation patterns of retina-specific genes.

    Shannath L Merbs

    Full Text Available Many studies have demonstrated that epigenetic mechanisms are important in the regulation of gene expression during embryogenesis, gametogenesis, and other forms of tissue-specific gene regulation. We sought to explore the possible role of epigenetics, specifically DNA methylation, in the establishment and maintenance of cell type-restricted gene expression in the retina. To assess the relationship between DNA methylation status and expression level of retinal genes, bisulfite sequence analysis of the 1000 bp region around the transcription start sites (TSS of representative rod and cone photoreceptor-specific genes and gene expression analysis were performed in the WERI and Y79 human retinoblastoma cell lines. Next, the homologous genes in mouse were bisulfite sequenced in the retina and in non-expressing tissues. Finally, bisulfite sequencing was performed on isolated photoreceptor and non-photoreceptor retinal cells isolated by laser capture microdissection. Differential methylation of rhodopsin (RHO, retinal binding protein 3 (RBP3, IRBP cone opsin, short-wave-sensitive (OPN1SW, cone opsin, middle-wave-sensitive (OPN1MW, and cone opsin, long-wave-sensitive (OPN1LW was found in the retinoblastoma cell lines that inversely correlated with gene expression levels. Similarly, we found tissue-specific hypomethylation of the promoter region of Rho and Rbp3 in mouse retina as compared to non-expressing tissues, and also observed hypomethylation of retinal-expressed microRNAs. The Rho and Rbp3 promoter regions were unmethylated in expressing photoreceptor cells and methylated in non-expressing, non-photoreceptor cells from the inner nuclear layer. A third regional hypomethylation pattern of photoreceptor-specific genes was seen in a subpopulation of non-expressing photoreceptors (Rho in cones from the Nrl -/- mouse and Opn1sw in rods. These results demonstrate that a number of photoreceptor-specific genes have cell-specific differential DNA

  12. DNA methylation profiling for a confirmatory test for blood, saliva, semen, vaginal fluid and menstrual blood.

    Lee, Hwan Young; Jung, Sang-Eun; Lee, Eun Hee; Yang, Woo Ick; Shin, Kyoung-Jin

    2016-09-01

    The ability to predict the type of tissues or cells from molecular profiles of crime scene samples has important practical implications in forensics. A previously reported multiplex assay using DNA methylation markers could only discriminate between 4 types of body fluids: blood, saliva, semen, and the body fluid which originates from female reproductive organ. In the present study, we selected 15 menstrual blood-specific CpG marker candidates based on analysis of 12 genome-wide DNA methylation profiles of vaginal fluid and menstrual blood. The menstrual blood-specificity of the candidate markers was confirmed by comparison with HumanMethylation450 BeadChip array data obtained for 58 samples including 12 blood, 12 saliva, 12 semen, 3 vaginal fluid, and 19 skin epidermis samples. Among 15CpG marker candidates, 3 were located in the promoter region of the SLC26A10 gene, and 2 of them (cg09696411 and cg18069290) showed high menstrual blood specificity. DNA methylation at the 2CpG markers was further tested by targeted bisulfite sequencing of 461 additional samples including 49 blood, 52 saliva, 34 semen, 125 vaginal fluid, and 201 menstrual blood. Because the 2 markers showed menstrual blood-specific methylation patterns, we modified our previous multiplex methylation SNaPshot reaction to include these 2 markers. In addition, a blood marker cg01543184 with cross reactivity to semen was replaced with cg08792630, and a semen-specific unmethylation marker cg17621389 was removed. The resultant multiplex methylation SNaPshot allowed positive identification of blood, saliva, semen, vaginal fluid and menstrual blood using the 9CpG markers which show a methylation signal only in the target body fluids. Because of the complexity in cell composition, menstrual bloods produced DNA methylation profiles that vary with menstrual cycle and sample collection methods, which are expected to provide more insight into forensic menstrual blood test. Moreover, because the developed

  13. Enhancing Stakeholder Acceptance Of Bioremediation Technologies

    This project inquired into the judgments and beliefs of people living near DOE reservations and facilities at Oak Ridge, Tennessee; Hanford, Washington; and Los Alamos, Tennessee about bioremediation of subsurface contamination. The purpose of the investigation was to identify strategies based on these judgments and beliefs for enhancing public support of bioremediation. Several methods were used to collect and analyze data including content analysis of transcripts of face-to-face personal interviews, factor analysis of subjective perspectives using Q methodology, and statistical analysis of results from a large-sample randomized telephone survey. Content analysis of interview transcripts identified themes about public perceptions and constructions of contamination risk, risk management, and risk managers. This analysis revealed that those who have no employment relationship at the sites and are not engaged in technical professions are most concerned about contamination risks. We also found that most interviewees are unfamiliar with subsurface contamination risks and how they can be reduced, believe they have little control over exposure, are frustrated with the lack of progress in remediation, are concerned about a lack of commitment of DOE to full remediation, and distrust site managers to act in the public interest. Concern is also expressed over frequent site management turnover, excessive secrecy, ineffective and biased communication, perceived attempts to talk the public into accepting risk, and apparent lack of concern about community welfare. In the telephone survey, we asked respondents who were aware of site contamination about their perceptions of risk from exposure to subsurface contamination. Response analysis revealed that most people believe that they are at significant risk from subsurface contamination but they acknowledge that more education is needed to calibrate risk perceptions against scientific risk assessments. Most rate their personal

  14. ENHANCING STAKEHOLDER ACCEPTANCE OF BIOREMEDIATION TECHNOLOGIES

    Focht, Will; Albright, Matt; Anex, Robert P., Jr., ed.

    2009-04-21

    This project inquired into the judgments and beliefs of people living near DOE reservations and facilities at Oak Ridge, Tennessee; Hanford, Washington; and Los Alamos, Tennessee about bioremediation of subsurface contamination. The purpose of the investigation was to identify strategies based on these judgments and beliefs for enhancing public support of bioremediation. Several methods were used to collect and analyze data including content analysis of transcripts of face-to-face personal interviews, factor analysis of subjective perspectives using Q methodology, and statistical analysis of results from a large-sample randomized telephone survey. Content analysis of interview transcripts identified themes about public perceptions and constructions of contamination risk, risk management, and risk managers. This analysis revealed that those who have no employment relationship at the sites and are not engaged in technical professions are most concerned about contamination risks. We also found that most interviewees are unfamiliar with subsurface contamination risks and how they can be reduced, believe they have little control over exposure, are frustrated with the lack of progress in remediation, are concerned about a lack of commitment of DOE to full remediation, and distrust site managers to act in the public interest. Concern is also expressed over frequent site management turnover, excessive secrecy, ineffective and biased communication, perceived attempts to talk the public into accepting risk, and apparent lack of concern about community welfare. In the telephone survey, we asked respondents who were aware of site contamination about their perceptions of risk from exposure to subsurface contamination. Response analysis revealed that most people believe that they are at significant risk from subsurface contamination but they acknowledge that more education is needed to calibrate risk perceptions against scientific risk assessments. Most rate their personal

  15. DNA loops and semicatenated DNA junctions

    Strauss François; Gaillard Claire

    2000-01-01

    Abstract Background Alternative DNA conformations are of particular interest as potential signals to mark important sites on the genome. The structural variability of CA microsatellites is particularly pronounced; these are repetitive poly(CA) · poly(TG) DNA sequences spread in all eukaryotic genomes as tracts of up to 60 base pairs long. Many in vitro studies have shown that the structure of poly(CA) · poly(TG) can vary markedly from the classical right handed DNA double helix and adopt dive...

  16. Mitochondrial Dna Replacement Versus Nuclear Dna Persistence

    Serva, Maurizio

    2006-01-01

    In this paper we consider two populations whose generations are not overlapping and whose size is large. The number of males and females in both populations is constant. Any generation is replaced by a new one and any individual has two parents for what concerns nuclear DNA and a single one (the mother) for what concerns mtDNA. Moreover, at any generation some individuals migrate from the first population to the second. In a finite random time $T$, the mtDNA of the second population is comple...

  17. Fast phylogenetic DNA barcoding

    Terkelsen, Kasper Munch; Boomsma, Wouter Krogh; Willerslev, Eske;

    2008-01-01

    We present a heuristic approach to the DNA assignment problem based on phylogenetic inferences using constrained neighbour joining and non-parametric bootstrapping. We show that this method performs as well as the more computationally intensive full Bayesian approach in an analysis of 500 insect...... DNA sequences obtained from GenBank. We also analyse a previously published dataset of environmental DNA sequences from soil from New Zealand and Siberia, and use these data to illustrate the fact that statistical approaches to the DNA assignment problem allow for more appropriate criteria...... for determining the taxonomic level at which a particular DNA sequence can be assigned....

  18. Electronic Transport in DNA

    Klotsa, Daphne; Römer, Rudolf A.; Turner, Matthew S.

    2005-01-01

    We study the electronic properties of DNA by way of a tight-binding model applied to four particular DNA sequences. The charge transfer properties are presented in terms of localisation lengths, crudely speaking the length over which electrons travel. Various types of disorder, including random potentials, are employed to account for different real environments. We have performed calculations on poly(dG)-poly(dC), telomeric-DNA, random-ATGC DNA and lambda-DNA. We find that random and lambda-D...

  19. DNA in Nanoscale Electronics

    Slinker, Jason

    2012-10-01

    DNA, the quintessential molecule of life, possesses a number of attractive properties for use in nanoscale circuits. Charge transport (CT) through DNA itself is of both fundamental and practical interest. Fundamentally, DNA has a unique configuration of π-stacked bases in a well ordered, double helical structure. Given its unparalleled importance to life processes and its arrangement of conjugated subunits, DNA has been a compelling target of conductivity studies. In addition, further understanding of DNA CT will elucidate the biological implications of this process and advance its use in sensing technologies. We have investigated the fundamentals of DNA CT by measuring the electrochemistry of DNA monolayers under biologically-relevant conditions. We have uncovered both fundamental kinetic parameters to distinguish between competing models of operation as well as the practical implications of DNA CT for sensing. Furthermore, we are leveraging our studies of DNA conductivity for the manufacture of nanoscale circuits. We are investigating the electrical properties and self-assembly of DNA nanowires containing artificial base pair surrogates, which can be prepared through low cost and high throughput automated DNA synthesis. This unique and economically viable approach will establish a new paradigm for the scalable manufacture of nanoscale semiconductor devices.

  20. DNA: Structure and function

    Sinden, Richard R.; E. Pearson, Christopher; N. Potaman, Vladimir;

    1998-01-01

    This chapter discusses the structure and function of DNA. DNA occupies a critical role in cells, because it is the source of all intrinsic genetic information. Chemically, DNA is a very stable molecule, a characteristic important for a macromolecule that may have to persist in an intact form for a...... long period of time before its information is accessed by the cell. Although DNA plays a critical role as an informational storage molecule, it is by no means as unexciting as a computer tape or disk drive. The structure of the DNA described by Watson and Crick in 1953 is a right handed helix of two...... individual antiparallel DNA strands. Hydrogen bonds provide specificity that allows pairing between the complementary bases (A.T and G.C) in opposite strands. Base stacking occurs near the center of the DNA helix and provides a great deal of stability to the helix (in addition to hydrogen bonding). The sugar...

  1. DNA from keratinous tissue

    Olsen, Maia E.; Bengtsson, Camilla Friis; Bertelsen, Mads Frost;

    2012-01-01

    Although good quality DNA can be recovered from the base of the calamus of freshly sampled feathers, as from other fully keratinized tissues such as nail or hair shaft, the quality and quantity of DNA in the majority of feather structures is much poorer. Little research has been performed...... to characterize the quality of this DNA is, and thus what a researcher might be able to achieve when using feathers as a source of DNA. In this review, we expand on our companion article detailing the quality of DNA in nail and hair, by synthesizing published, and new preliminary genetic data obtained from...... feathers. As with nail and hair, we demonstrate that although DNA can, in general, be recovered from all parts of the feather, the quality of such DNA varies. As such, although one can expect a priori that genetic analyses are possible on the feather, for PCR based analyses, it is extremely difficult...

  2. Biophysics of DNA

    Vologodskii, Alexander

    2015-01-01

    Surveying the last sixty years of research, this book describes the physical properties of DNA in the context of its biological functioning. It is designed to enable both students and researchers of molecular biology, biochemistry and physics to better understand the biophysics of DNA, addressing key questions and facilitating further research. The chapters integrate theoretical and experimental approaches, emphasising throughout the importance of a quantitative knowledge of physical properties in building and analysing models of DNA functioning. For example, the book shows how the relationship between DNA mechanical properties and the sequence specificity of DNA-protein binding can be analyzed quantitatively by using our current knowledge of the physical and structural properties of DNA. Theoretical models and experimental methods in the field are critically considered to enable the reader to engage effectively with the current scientific literature on the physical properties of DNA.

  3. On acceptance criteria for Mochovce startup physical tests

    New acceptance criteria are discussed for physical startup tests of Mochovce Units 1, 2, at the stage of their development. The acceptance criteria determination is based on statistical processing of differences between the experimental and theoretical values of neutron-physical characteristics. The experimental values were taken from physical startup tests of Bohunice Unit 1, 2 and 4 and Dukovany Unit 1 - 4. The theoretical values were calculated by code BIPR-7. The acceptance criteria were established for critical boron concentration, core loading symmetry measurement, boric acid worth, temperature reactivity coefficient, 'ejected' control rod worth, pressure reactivity coefficient and power reactivity coefficient. The new acceptance criteria were applied to evaluate results of physical startup tests of Mochove Unit 1 in June 1998. The acceptance criteria showed to be adequate. The summary results of statistical processing and the comparison of earlier, new and US acceptance criteria for physical startup tests are presented. (author)

  4. A study on the acceptance criteria of radioactive waste form

    It is essential to accept well solidified and packaged waste forms for the safety during the operational and post operational phase in the repository, and for this, waste the acceptance criterion is necessary for the distinction of the well solidified and packaged waste form. The objective of this report is to provide the preliminary acceptance criteria to help the later establishment of final acceptance criteria. The following factors were considered for establishing the preliminary waste acceptance criteria. 1) Matrix and waste form characteristics 2) the type of repository and its characteristics 3) establishment procedure of acceptance criteria and its technical background From this study, a qualitative preliminary criterion including the radionuclide contents, surface dose, surface contamination and so on was established. (Author)

  5. Acceptance and marketability of the food irradiation technology

    The food irradiation technology has been struggling for forty years for acceptance and utilization. The issue of consumer acceptance is addressed and judged not to be the critical factor in terms of priority and timing. The producing/processing marketing industries must first accept the technology for valid business or social reasons. If they become convinced that they cannot afford to pass up the technology, they will accept the process and offer irradiated products. These industries understand public acceptance and use professionals in market development and advertising to achieve consumption of their products. Consumer acceptance can best be developed by the food industry, while the research and development community, in concert with national and international agencies, can and should provide the industry with every assistance in reaching a consensus on the validity of food irradiation as an appropriate and useful technology

  6. Amyloid protein-mediated differential DNA methylation status regulates gene expression in Alzheimer’s disease model cell line

    Highlights: ► Genome-wide DNA methylation pattern in Alzheimer’s disease model cell line. ► Integrated analysis of CpG methylation and mRNA expression profiles. ► Identify three Swedish mutant target genes; CTIF, NXT2 and DDR2 gene. ► The effect of Swedish mutation on alteration of DNA methylation and gene expression. -- Abstract: The Swedish mutation of amyloid precursor protein (APP-sw) has been reported to dramatically increase beta amyloid production through aberrant cleavage at the beta secretase site, causing early-onset Alzheimer’s disease (AD). DNA methylation has been reported to be associated with AD pathogenesis, but the underlying molecular mechanism of APP-sw-mediated epigenetic alterations in AD pathogenesis remains largely unknown. We analyzed genome-wide interplay between promoter CpG DNA methylation and gene expression in an APP-sw-expressing AD model cell line. To identify genes whose expression was regulated by DNA methylation status, we performed integrated analysis of CpG methylation and mRNA expression profiles, and identified three target genes of the APP-sw mutant; hypomethylated CTIF (CBP80/CBP20-dependent translation initiation factor) and NXT2 (nuclear exporting factor 2), and hypermethylated DDR2 (discoidin domain receptor 2). Treatment with the demethylating agent 5-aza-2′-deoxycytidine restored mRNA expression of these three genes, implying methylation-dependent transcriptional regulation. The profound alteration in the methylation status was detected at the −435, −295, and −271 CpG sites of CTIF, and at the −505 to −341 region in the promoter of DDR2. In the promoter region of NXT2, only one CpG site located at −432 was differentially unmethylated in APP-sw cells. Thus, we demonstrated the effect of the APP-sw mutation on alteration of DNA methylation and subsequent gene expression. This epigenetic regulatory mechanism may contribute to the pathogenesis of AD.

  7. Nuclear Power and Radiation in Public Acceptance

    accents correctly when enlightening a number of problems, especially in women's audience, in order to show the situation in power truly and objectively and to increase the level of acceptance of nuclear power as the necessary and sufficiently safe energy source for the environment. (author)

  8. Factors Affecting Acceptance of Smartphone Application for Management of Obesity

    Jeon, Eunjoo; Park, Hyeoun-Ae

    2015-01-01

    Objectives The factors affecting the acceptance of mobile obesity-management applications (apps) by the public were analyzed using a mobile healthcare system (MHS) technology acceptance model (TAM). Methods The subjects who participated in this study were Android smartphone users who had an intent to manage their weight. They used the obesity-management app for two weeks, and then completed an 18-item survey designed to determine the factors influencing the acceptance of the app. Three questi...

  9. Card acceptance and surcharging: the role of costs and competition

    2011-01-01

    The payment cards market is a two-sided market. Cost sensitivity of both consumers and merchants for card services influences total demand. Survey data of Dutch merchants shows that costs, and competition affect acceptance as well as surcharging decisions. Merchants who find payment cards expensive are less likely to accept them and more likely to surcharge their customers for using them. Merchants who face any competition accept debit card payments relatively more often than merchants with m...

  10. Italian Consumer Acceptance of Nutritionally Enhanced GM Food

    Canavari, Maurizio; Tisselli, Farid; Nayga, Rodolfo M.; Scarpa, Riccardo

    2009-01-01

    The aim of this article is to evaluate Italian consumers’ acceptance and willingness to purchase GM foods based on the type of benefit (input vs output trait) and product (plant based vs animal based). Two surveys were administered in two consecutive years (2004 and 2005) and the data used to test for possible changes in consumer acceptance. The results of a multinomial logit analysis suggest that on average consumer acceptance for plant-based GM food was higher in 2005. This study confirmed ...

  11. RECENT RELATED RESEARCH IN TECHNOLOGY ACCEPTANCE MODEL: A LITERATURE REVIEW

    Shih-Chih Chen; Shing-Han Li; Chien-Yi Li

    2011-01-01

    Technology Acceptance Model is widely applied to access users’ usage in various information system/information technology areas. Learning the critical role of Technology Acceptance Model can guide researchers to design different users’ interface for different online customers, and consequently achieve high user usage in different application areas. This study reviewed 24 studies to understand the past, now and future of Technology Acceptance Model. We discussed the related studies to ...

  12. Empirical Study and Model of User Acceptance for Personalized Recommendation

    Zheng Hua

    2013-01-01

    Personalized recommendation technology plays an important role in the current e-commerce system, but the user willingness to accept the personalized recommendation and its influencing factors need to be study. In this study, the Theory of Reasoned Action (TRA) and Technology Acceptance Model (TAM) are used to construct a user acceptance model of personalized recommendation which tested by the empirical method. The results show that perceived usefulness, perceived ease of use, subjective rules...

  13. Acceptability and processing of long-distance dependencies in Danish

    Poulsen, Mads

    2008-01-01

    Long-distance dependencies have been the object of much theoretical interest in the Scandinavian languages and in general, but the empirical foundation for theorizing has been limited. The present paper investigates extraction from complement and adverbial clauses in Danish using acceptability...... acceptable. It is concluded that under the conditions investigated extraction from adverbial clauses in Danish is associated with a processing cost and very low acceptability ratings, despite semantic cohesion....

  14. The Customer Acceptance of Online Insurance in Malaysia

    Tan, Chye Ting

    2012-01-01

    Over a decade ago, when Internet technology became a major influence in people’s life; where information could flow freely and rapidly, Information Technology significantly changed the way business is done. Many researchers have dedicated effort and time into the research of technology acceptance among consumer. As a result, many theories and model of technology acceptance are developed to predict and explain user behavior. This research draws upon the Technology Acceptance Model to investiga...

  15. A School Choice Compromise: Between Immediate and Deferred Acceptance

    Harless, Patrick

    2014-01-01

    School assignment procedures aim to improve student welfare, but must balance efficiency and equity goals and provide incentives for students to report their preferences truthfully. Debate centers largely on two rules: immediate acceptance (IA), the so-called Boston mechanism, and deferred acceptance (DA). IA's strength is efficiency, while DA is touted for its superior strategic properties. Thinking of these as extremes, we advocate a compromise rule, immediate-acceptance-with-skips (IA+), ...

  16. Understanding and changing children’s sensory acceptance for vegetables

    Poelman, A.A.M.

    2016-01-01

    Vegetable intake of children is well below recommendations in Australia and in most other western countries. Vegetables are the food category least liked by children. As acceptance is a key driver of intake, strategies are needed to increase children’s acceptance of vegetables. The present thesis was directed at understanding and changing children’s acceptance for vegetables, focusing on strategies that could be employed by parents in the home environment. The research was conduct...

  17. Nevada Test Site Waste Acceptance Criteria, December 2000

    NONE

    2000-12-01

    This document establishes the US Department of Energy, Nevada Operations Office waste acceptance criteria. The waste acceptance criteria provides the requirements, terms, and conditions under which the Nevada Test Site will accept low-level radioactive waste and mixed waste for disposal. It includes requirements for the generator waste certification program, characterization, traceability, waste form, packaging, and transfer. The criteria apply to radioactive waste received at the Nevada Test Site Area 3 and Area 5 Radioactive Waste Management Sites for storage or disposal.

  18. Nevada Test Site Waste Acceptance Criteria, December 2000

    This document establishes the US Department of Energy, Nevada Operations Office waste acceptance criteria. The waste acceptance criteria provides the requirements, terms, and conditions under which the Nevada Test Site will accept low-level radioactive waste and mixed waste for disposal. It includes requirements for the generator waste certification program, characterization, traceability, waste form, packaging, and transfer. The criteria apply to radioactive waste received at the Nevada Test Site Area 3 and Area 5 Radioactive Waste Management Sites for storage or disposal

  19. Internet banking acceptance model: Cross-market examination

    Alsajjan, B; C. Dennis

    2009-01-01

    This article proposes a revised technology acceptance model to measure consumers’ acceptance of Internet banking, the Internet Banking Acceptance Model (IBAM). Data was collected from 618 university students in the United Kingdom and Saudi Arabia. The results suggest the importance of attitude, such that attitude and behavioral intentions emerge as a single factor, denoted as “attitudinal intentions” (AI). Structural equation modeling confirms the fit of the model, in which per...

  20. Influencing Acceptability of Parent Training Interventions Through Treatment Rationales

    Chase, Trisha

    2015-01-01

    Parent training is an effective intervention for parents of children with a variety of childhood disorders, and parents often view behavioral parent training as acceptable. Explanations and rationales for parent training are commonly provided at the beginning of treatment. However, there is little research regarding how rationales may influence acceptability. There is also limited information on whether fathers and mothers judge the acceptability of parent training differently. The purpose of...

  1. DNA Hairpins: Fuel for Autonomous DNA Devices

    Green, Simon J.; Lubrich, Daniel; Turberfield, Andrew J.

    2006-01-01

    We present a study of the hybridization of complementary DNA hairpin loops, with particular reference to their use as fuel for autonomous DNA devices. The rate of spontaneous hybridization between complementary hairpins can be reduced by increasing the neck length or decreasing the loop length. Hairpins with larger loops rapidly form long-lived kissed complexes. Hairpin loops may be opened by strand displacement using an opening strand that contains the same sequence as half of the neck and a...

  2. Novel DNA probes for sensitive DNA detection

    Richardson, James Alistair

    2010-01-01

    The ability to detect and interrogate DNA sequences allows further understanding and diagnosis of genetic disease. The ability to perform such analysis of genetic material requires highly selective and reliable technologies. Furthermore techniques which can use simple and cheap equipment allow the use of such technologies for point of care analysis. Described in this thesis are two novel DNA probe systems designed for mutation discrimination and sequence recognition of PCR pro...

  3. Fidelity of DNA polymerases in DNA amplification.

    Keohavong, P; Thilly, W G

    1989-01-01

    Denaturing gradient gel electrophoresis (DGGE) was used to separate and isolate the products of DNA amplification by polymerase chain reaction (PCR). The strategy permitted direct enumeration and identification of point mutations created by T4, modified T7, Klenow fragment of polymerase I, and Thermus aquaticus (Taq) DNA polymerases. Incorrectly synthesized sequences were separated from the wild type by DGGE as mutant/wild-type heteroduplexes and the heteroduplex fraction was used to calculat...

  4. DNA degradation and Apoptosis : DNA degradation

    Torriglia, Alicia; Padron, Laura

    2005-01-01

    Apoptosis, is a form of programmed cell death essential for the development and maintenance of multicellular organisms. DNA degradation is one of the hallmarks of apoptosis. The central component of the apoptotic machinery is a proteolytic system involving caspases and non-caspases proteases. CAD, a caspase-activated DNase, is the endonuclease responsible for DNA degradation during caspase-dependent apoptosis. The relationship between non-caspase proteases and endonucleases is less clear and ...

  5. Nature preservation acceptance model applied to tanker oil spill simulations

    Friis-Hansen, Peter; Ditlevsen, Ove Dalager

    2003-01-01

    This paper emphazises the adverse event categorization principle in risk acceptance analysis, and suggests the use of a standard type risk profile of lognormal type for each category of adverse events. The risk profile for a specified category of adverse events and corresponding to a given...... of the single oil spill, a risk profile for the costs is obtained that is indistinguishable from the standard lognormal risk profile.Finally the question of formulating a public risk acceptance criterion is addressed following Ditlevsen, and it is argued that a Nature Preservation Willingness Index...... risk acceptance criterion for the pollution of the environment. This NPWI acceptance criterion is applied to the oil spill example....

  6. Local acceptance of existing biogas plants in Switzerland

    After the Swiss government's decision to decommission its five nuclear power plants by 2035, energy production from wind, biomass, biogas and photovoltaic is expected to increase significantly. Due to its many aspects of a direct democracy, high levels of public acceptance are necessary if a substantial increase in new renewable energy power plants is to be achieved in Switzerland. A survey of 502 citizens living near 19 biogas plants was conducted as the basis for using structural equation modeling to measure the effects of perceived benefits, perceived costs, trust towards the plant operator, perceived smell, information received and participation options on citizens’ acceptance of “their” biogas plant. Results show that local acceptance towards existing biogas power plants is relatively high in Switzerland. Perceived benefits and costs as well as trust towards the plant operator are highly correlated and have a significant effect on local acceptance. While smell perception and information received had a significant effect on local acceptance as well, no such effect was found for participation options. Reasons for the non-impact of participation options on local acceptance are discussed, and pathways for future research are presented. - Highlights: • Acceptance of biogas plants by local residents in Switzerland is relatively high. • Local acceptance is highly affected by perceived outcomes and citizens’ trust. • Smell perception increases perceived costs and reduces perceived benefits and trust. • Information offers reduce perceived costs and increase trust and perceived benefits. • Participation offers do not have any effect on local acceptance

  7. Why Should I Accept Ads on my Mobile Phone? Factors Affecting the Acceptance by Brazilian Teenagers

    Luis Fernando Hor-Meyll; Marcel Correia de Lima; Jorge Brantes Ferreira

    2014-01-01

    Among the more than six billion cellular phone lines in the world, around 220 million are found in Brazil. These numbers draw the attention of companies and academics, seeking to understand how cell phones can be used as an advertising media. This study sought to discover factors that could affect the intention of Brazilian teenagers to accept advertisements via SMS messages. A survey was conducted on a sample of 385 students of a high school in Rio de Janeiro. The results of an analysis via ...

  8. Oppression through acceptance?: predicting rape myth acceptance and attitudes toward rape victims.

    Hockett, Jericho M; Saucier, Donald A; Hoffman, Bethany H; Smith, Sara J; Craig, Adam W

    2009-08-01

    Feminist theories of rape motivation are based on research suggesting a relationship between dominance and sexual aggression. However, the relationship between dominance and rape myth acceptance (RMA), a predictor of rape proclivity and sexual aggression and a key component in feminist theory, is understudied. The current study tests the hypotheses that individuals' scores on sex-based oppression and intergroup dominance measures will improve the predictive models for RMA and attitudes toward rape and rape victims. The hypotheses are supported. Individuals' general intergroup dominance and sex-based oppression attitudes provide significant unique prediction beyond previously studied predictors of attitudes about rape and rape victims. PMID:19506093

  9. mTOR inhibition and levels of the DNA repair protein MGMT in T98G glioblastoma cells

    2014-01-01

    Background Glioblastoma multiforme (GBM), the most common and most aggressive type of primary adult brain tumour, responds poorly to conventional treatment. Temozolomide (TMZ) chemotherapy remains the most commonly used treatment, despite a large proportion of tumours displaying TMZ resistance. 60% of GBM tumours have unmethylated MGMT promoter regions, resulting in an overexpression of the DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT), which is responsible for tumour resistance to TMZ chemotherapy. Tumours also often exhibit hyperactive PI3-kinase/mTOR signalling, which enables them to resynthesise proteins quickly. Since MGMT is a suicide protein that is degraded upon binding to and repairing TMZ-induced O6-methylguanine adducts, it has been hypothesized that inhibition of translation via the mTOR signalling pathway could generate a tumour-specific reduction in MGMT protein and increase TMZ sensitivity. Methods MGMT was monitored at the post-transcriptional, translational and protein levels, to determine what effect mTOR inhibition was having on MGMT protein expression in vitro. Results We show that inhibiting mTOR signalling is indeed associated with acute inhibition of protein synthesis. Western blots show that despite this, relative to loading control proteins, steady state levels of MGMT protein increased and MGMT mRNA was retained in heavy polysomes. Whilst TMZ treatment resulted in maintained MGMT protein levels, concomitant treatment of T98G cells with TMZ and KU0063794 resulted in increased MGMT protein levels without changes in total mRNA levels. Conclusions These in vitro data suggest that, counterintuitively, mTOR inhibition may not be a useful adjunct to TMZ therapy and that more investigation is needed before applying mTOR inhibitors in a clinical setting. PMID:24909675

  10. Forensic DNA analysis.

    McDonald, Jessica; Lehman, Donald C

    2012-01-01

    Before the routine use of DNA profiling, blood typing was an important forensic tool. However, blood typing was not very discriminating. For example, roughly 30% of the United States population has type A-positive blood. Therefore, if A-positive blood were found at a crime scene, it could have come from 30% of the population. DNA profiling has a much better ability for discrimination. Forensic laboratories no longer routinely determine blood type. If blood is found at a crime scene, DNA profiling is performed. From Jeffrey's discovery of DNA fingerprinting to the development of PCR of STRs to the formation of DNA databases, our knowledge of DNA and DNA profiling have expanded greatly. Also, the applications for which we use DNA profiling have increased. DNA profiling is not just used for criminal case work, but it has expanded to encompass paternity testing, disaster victim identification, monitoring bone marrow transplants, detecting fetal cells in a mother's blood, tracing human history, and a multitude of other areas. The future of DNA profiling looks expansive with the development of newer instrumentation and techniques. PMID:22693781

  11. CpG DNA as a vaccine adjuvant

    Bode, Christian; Zhao, Gan; Steinhagen, Folkert; Kinjo, Takeshi; Klinman, Dennis M.

    2011-01-01

    Synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs trigger cells that express Toll-like receptor 9 (including human plasmacytoid dendritic cells and B cells) to mount an innate immune response characterized by the production of Th1 and proinflammatory cytokines. When used as vaccine adjuvants, CpG ODNs improve the function of professional antigen-presenting cells and boost the generation of humoral and cellular vaccine-specific immune responses. These effects are optimi...

  12. Regulatory process and effects on public acceptance

    In Switzerland four Federal laws are involved in controlling nuclear energy. Two of them are applied on the Federal level and entail a single Federal licensing procedure (peaceful uses of atomic energy; protection of nature and landscape). The other two laws are applied on the cantonal level and entail two different cantonal licensing procedures (protection of waters; industry, crafts and trade). Even though these four laws together cover all aspects of the protection of man, the environment and the countryside, nevertheless: - the legal situation and the overall licensing system are complex; they are unintelligible to the man in the street and offer a lawyer numerous possibilities for bringing in appeals; - decisions affecting the three licensing procedures are taken at different levels; and - the nuclear law contains a clause obliging the Federal authorities to treat information on the nuclear aspects of projects confidentially. Although this state of affairs has not appreciably influenced acceptance by the population living near the two operating power plants and the two that are under construction, it has led, in the case of two of the five new projects, to consistently mounting opposition on the part of the local population, opposition which in some cases has reached a very high pitch. In 1975, there was one case where the site was occupied for several months, a violation of the law. Similarly, the opposition which was latent in the rest of Switzerland until two years ago has reached the stage of initiatives: petitions on nuclear energy have been filed in some cantons, and a ''Federal popular initiative to safeguard the people's rights and safety during the construction and operation of atomic facilities'' has been launched. Since the four laws covering nuclear power plants were adopted on the Federal level, it would seem that the cantonal initiatives lack a legal basis. On the other hand the Federal initiative, which demands in particular that powers of

  13. Physics acceptance and QA procedures for IMRT

    QA program for DMLC. We distinguish between the preliminary preparations for clinical DMLC implementation (i.e., acceptance testing and commissioning), the routine QA of DMLC performance (machine QA), and the verification of patient-specific IM fields using DMLC (treatment QA). Since our initial DMLC implementation in 1995, there have been a number of incremental improvements to our overall QA program. These include methods of mechanical and dosimetric measurements, and software tools developed either by us or by the manufacturer. The use of EPID (electronic portal imaging devise), which potentially provide a more efficient, but certainly not 'cost-free' method, awaits improvements in the speed of data acquisition and in spatial resolution. Our current approach integrates existing methods, combining periodic QA and computer verification, to provide the necessary quality assurance in a safe and efficient manner. Copyright (2001) Australasian College of Physical Scientists and Engineers in Medicine

  14. Acceptance Probability (P a) Analysis for Process Validation Lifecycle Stages.

    Alsmeyer, Daniel; Pazhayattil, Ajay; Chen, Shu; Munaretto, Francesco; Hye, Maksuda; Sanghvi, Pradeep

    2016-04-01

    This paper introduces an innovative statistical approach towards understanding how variation impacts the acceptance criteria of quality attributes. Because of more complex stage-wise acceptance criteria, traditional process capability measures are inadequate for general application in the pharmaceutical industry. The probability of acceptance concept provides a clear measure, derived from specific acceptance criteria for each quality attribute. In line with the 2011 FDA Guidance, this approach systematically evaluates data and scientifically establishes evidence that a process is capable of consistently delivering quality product. The probability of acceptance provides a direct and readily understandable indication of product risk. As with traditional capability indices, the acceptance probability approach assumes that underlying data distributions are normal. The computational solutions for dosage uniformity and dissolution acceptance criteria are readily applicable. For dosage uniformity, the expected AV range may be determined using the s lo and s hi values along with the worst case estimates of the mean. This approach permits a risk-based assessment of future batch performance of the critical quality attributes. The concept is also readily applicable to sterile/non sterile liquid dose products. Quality attributes such as deliverable volume and assay per spray have stage-wise acceptance that can be converted into an acceptance probability. Accepted statistical guidelines indicate processes with C pk > 1.33 as performing well within statistical control and those with C pk  1.33 is associated with a centered process that will statistically produce less than 63 defective units per million. This is equivalent to an acceptance probability of >99.99%. PMID:26024723

  15. What is meant by the term acceptance of technology and locating the acceptance of the CCS Technology?

    Harz, Mario; Vesper, Anton

    2013-04-01

    abstract: The formal language of logic expresses concepts and statements exactly. The logic of relations can serve as an important ressource for the philosophical analysis of technology and the construction of philosophical propositions about acceptance of technology. The theory of logical relations is used to investigate the theoretical structure of how acceptance of technologies can be revealed. The term "ordered tuple" helps to define the basis of the concept of logical relations. The term "acceptance of technology" refers to neither a thing nor a property; but to a complex relationship. The research refers to the study of the properties of this complex relationship. It examines the properties of reflexivity, total reflexivity, symmetry, transitivity, irreflexivity and asymmetry. Using these properties and the rules for forming converses-relations and partial-relations the question is analyzed: What, in general, is meant by the term "acceptance of technology?" These properties have been observed empirically at a discussion forum for the key players in the Brandenburg discourse on the acceptance of CCS technology. The meeting was held on the 8th of May 2012 in St. Nicholas Church, Cottbus (GER). The pragma-dialectical theory of argumentation is used to locate the acceptance of the CCS technology. With the ideal model of critical discussion as the methodological starting point the term "acceptance" can be defined in terms of the four meta-theoretical principles of the theory. That boils down to the findings that acceptance is the externalization of a positive commitment towards a proposition, acceptance is expressed by the speech act "to accept" and acceptance occurs in the dialogical, interactional setting of a critical discussion with the aim of resolving a difference of opinion. In the study differences of opinion about (descriptive, normative, evaluative) standpoints about the CCS technology from everyday problem-solving discussions are investigated. The

  16. DNA profiles from fingermarks.

    Templeton, Jennifer E L; Linacre, Adrian

    2014-11-01

    Criminal investigations would be considerably improved if DNA profiles could be routinely generated from single fingermarks. Here we report a direct DNA profiling method that was able to generate interpretable profiles from 71% of 170 fingermarks. The data are based on fingermarks from all 5 digits of 34 individuals. DNA was obtained from the fingermarks using a swab moistened with Triton-X, and the fibers were added directly to one of two commercial DNA profiling kits. All profiles were obtained without increasing the number of amplification cycles; therefore, our method is ideally suited for adoption by the forensic science community. We indicate the use of the technique in a criminal case in which a DNA profile was generated from a fingermark on tape that was wrapped around a drug seizure. Our direct DNA profiling approach is rapid and able to generate profiles from touched items when current forensic practices have little chance of success. PMID:25391915

  17. Electrocatalysis in DNA Sensors.

    Furst, Ariel; Hill, Michael G; Barton, Jacqueline K

    2014-12-14

    Electrocatalysis is often thought of solely in the inorganic realm, most often applied to energy conversion in fuel cells. However, the ever-growing field of bioelectrocatalysis has made great strides in advancing technology for both biofuel cells as well as biological detection platforms. Within the context of bioelectrocatalytic detection systems, DNA-based platforms are especially prevalent. One subset of these platforms, the one we have developed, takes advantage of the inherent charge transport properties of DNA. Electrocatalysis coupled with DNA-mediated charge transport has enabled specific and sensitive detection of lesions, mismatches and DNA-binding proteins. Even greater signal amplification from these platforms is now being achieved through the incorporation of a secondary electrode to the platform both for patterning DNA arrays and for detection. Here, we describe the evolution of this new DNA sensor technology. PMID:25435647

  18. User acceptance testing a step-by-step guide

    Hambling, Brian

    2013-01-01

    Every information system brought into service in every type of organisation requires user acceptance testing. This book is a hands-on manual for non-testing specialists to plan and carry out an effective acceptance test of an information system. It also identifies ways of making the process as simple and cost-effective as possible.

  19. User Acceptance of Information Technology: Theories and Models.

    Dillon, Andrew; Morris, Michael G.

    1996-01-01

    Reviews literature in user acceptance and resistance to information technology design and implementation. Examines innovation diffusion, technology design and implementation, human-computer interaction, and information systems. Concentrates on the determinants of user acceptance and resistance and emphasizes how researchers and developers can…

  20. Values, Perceived Risks and Benefits, and Acceptability of Nuclear Energy

    de Groot, Judith I. M.; Steg, Linda; Poortinga, Wouter

    2013-01-01

    We examined how personal values and perceptions of risks and benefits are associated with the acceptability of nuclear energy (NE). A theoretical model is tested in which beliefs about the risks and benefits of NE mediate the relationship between values and acceptability. The results showed that ego