WorldWideScience

Sample records for acari tetranychidae em

  1. Primeiro registro de Tetranychus urticae (Acari: Tetranychidae) em mudas de teca no Brasil

    Alexandre dos Santos; Vagner Aniceto Teixeira; Otávio Peres Filho; Milson Evaldo Serafin; Marçal Pedro Neto; Carlos Alberto da Cunha Oliveira

    2014-01-01

    O ácaro fitófago Tetranychus urticae (Kock, 1836) (Acari: Tetranychidae) foi relatado e suas injúrias caracterizadas pela primeira vez em viveiro de produção mudas de teca (Tectona grandis) no Brasil.

  2. Primeiro registro de Tetranychus urticae (Acari: Tetranychidae em mudas de teca no Brasil

    Alexandre dos Santos

    2014-07-01

    Full Text Available O ácaro fitófago Tetranychus urticae (Kock, 1836 (Acari: Tetranychidae foi relatado e suas injúrias caracterizadas pela primeira vez em viveiro de produção mudas de teca (Tectona grandis no Brasil.

  3. Temperature-dependent development and reproductive traits of <em>Tetranychus macfarlaneiem> (Acari: Tetranychidae)

    Ullah, Mohammad Shaef; Haque, Md. Ahsanul; Nachman, Gösta;

    2012-01-01

    Development and reproductive traits of Tetranychus macfarlanei Baker & Pritchard (Acari: Tetranychidae) were investigated on kidney bean, Phaseolus vulgaris L., at eleven constant temperatures. Tetranychus macfarlanei was able to develop and complete its life cycle at temperatures ranging from 17...

  4. Primeiro registro de Oligonychus yothersi (McGregor (Acari: Tetranychidae em Eucalyptus grandis Hill ex Maiden no Brasil First record of Oligonychus yothersi (McGregor (Acari: Tetranychidae on Eucalyptus grandis Hill ex Maiden in Brazil

    Fabrício Fagundes Pereira

    2005-08-01

    Full Text Available Relata-se a infestação de um ácaro-vermelho em mudas clonais de Eucalyptus grandis Hill ex Maiden, mantidas em casa de vegetação no município de Martinho Campos, Minas Gerais. O ácaro foi observado na parte superior das folhas que exibiam sinais de sucção de seiva e bronzeamento. Essas injúrias causaram desenvolvimento anormal e morte de plantas. O ácaro foi identificado como Oligonychus yothersi (McGregor (Acari: Tetranychidae, e isso representa o primeiro registro dessa espécie em mudas clonais de E. grandis no Brasil.An infestation of the red spider mite was reported in clone seedlings of Eucalyptus grandis Hill ex Maiden under greenhouse conditions, in the municipality of Martinho Campos, Minas Gerais State. The spider mite was found on the leaf upper faces with signs of sap suction and bronzing. Such injuries caused abnormal development and plant death. The spider mite was identified as Oligonychus yothersi (McGregor (Acari: Tetranychidae. This is the first record of O. yothersi on E. grandis seedlings in Brazil.

  5. Biologia e flutuação populacional de Mononychellus Planki (Mc Gregor) (Acari: Tetranychidae) em cultivares de soja Glycine Max (L.) Merr. e impacto do imidacloprido em aspectos biológicos do adulto.

    Siqueira, Fabio

    2012-01-01

    Resumo: Visando buscar cultivares de soja Glycine max (L.) Merr. com fatores de resistência ao ácaro verde Mononychellus planki (McGregor) (Acari: Tetranychidae) foram realizados estudos sobre a biologia em laboratório, flutuação populacional em diferentes cultivares durante duas safras e possíveis ações de agrotóxicos no crescimento populacional. Inicialmente foram realizados estudos de biologia de imaturos em dez cultivares BRS 133, BRS 134, BRS 282, BRS 284, BRS 245 RR, BMX Titan RR, IAC 1...

  6. The complete mitochondrial genome of Tetranychus truncatus Ehara (Acari: Tetranychidae).

    Chen, Da-Song; Jin, Peng-Yu; Hong, Xiao-Yue

    2016-01-01

    The complete mitochondrial genome of Tetranychus truncatus Ehara (Acari: Tetranychidae) is a typical circular DNA with length of 13,089 bp (GenBank accession number: KM111296). The genome contains all 13 protein-coding genes (PCGs), an A + T-rich region, two rRNA genes and 22 tRNA genes. The A + T content of the mitochondrial genome is 84.5%. The AT-skew is positive (0.032) while the GC-skew is negative (-0.058). The gene arrangement is conserved in T. urticae, Panonychus citri and P. ulmi which are in the same family (Tetranychidae). The A + T-rich region is only 43 bp in length with high A + T content (97.7%). All the PCGs start with typical ATD codons. Eight PCGs have complete TAA stop codons, while five PCGs have an incomplete stop codon (T). PMID:25162745

  7. Controle de Brevipalpus phoenicis (Geijskes, 1939 E Oligonychus ilicis (McGregor, 1917 (Acari: Tenuipalpidae, Tetranychidae em cafeeiro e o impacto sobre ácaros benéficos: I - abamectin e emamectin Control of Brevipalpus phoenicis (Geijskes, 1939 and Oligonychus ilicis (McGregor, 1917 (Acari: Tenuipalpidae, Tetranychidae in coffee plants and the impact on beneficial mites: I - Abamectin and emamectin

    Paulo Rebelles Reis

    2004-04-01

    Full Text Available O ácaro Brevipalpus phoenicis (Geijskes, 1939 (Acari: Tenuipalpidae é importante em cafeeiro (Coffea spp., por ser o vetor do vírus da mancha-anular, responsável por queda de folhas e má qualidade da bebida do café, e o ácaro-vermelho, Oligonychus ilicis (McGregor, 1917 (Acari: Tetranychidae, por reduzir a área foliar de fotossíntese. Alguns ácaros da família Phytoseiidae são eficientes predadores associados aos ácaros-praga. Com este trabalho teve-se como objetivo estudar o controle dos ácaros-praga e o impacto do abamectin e emamectin sobre fitoseídeos. Em laboratório, foram estudados os efeitos ovicida, tópico, residual, tópico mais residual aos ácaros-praga e a seletividade fisiológica aos fitoseídeos. Em semicampo, foi estudada a persistência dos produtos no controle dos ácaros-praga. O efeito ovicida foi avaliado em ovos no início e fim de incubação; os efeitos residual, tópico e tópico mais residual foram avaliados pela mortalidade de larvas, ninfas e adultos após 48 horas da aplicação, enquanto a persistência foi avaliada pela mortalidade até 30 dias após a pulverização. A seletividade aos ácaros fitoseídeos foi avaliada pelo efeito total às fêmeas adultas, em teste residual em superfície de vidro. Pelos resultados, verificou-se que abamectin e emamectin não possuem ação ovicida, para ambas as espécies de ácaros-praga estudadas. Considerando o efeito tópico mais residual, o abamectin e emamectin foram altamente eficientes no controle de larvas, ninfas e adultos de B. phoenicis; apenas abamectin foi eficiente no controle de O. ilicis. Abamectin foi levemente a moderadamente nocivo e emamectin mostrou-se inócuo a levemente nocivo aos fitoseídeos. Devido à eficiência de controle e seletividade a fitoseídeos, conclui-se que abamectin e emamectin podem ser utilizados em programas de manejo integrado do ácaro B. phoenicis, e abamectin para o manejo de B. phoenicis e O. ilicis em cafeeiro

  8. Parâmetros biológicos e tabela de vida de Tetranychus urticae (Acari: Tetranychidae em cultivares de mamão Biological parameters and life table of Tetranychus urticae (Acari: Tetranychidae at papaya cultivars

    Larissa Bernardino Moro

    2012-03-01

    Full Text Available Este trabalho foi realizado para avaliar o desenvolvimento e reprodução de Tetranychus urticae em cultivares comerciais de mamão Carica papaya. Foram utilizadas cultivares do grupo "Formosa" ("Tainung 01" e "Calimosa" e do grupo "Solo" ("Golden" e "Sunrise". Para iniciar o bioensaio, foi transferida uma fêmea fertilizada por disco de folha (n=50 repetições e retirado após um período de 12h, sendo avaliada a cada 12h, registrando-se o período de incubação, duração do estágio de imaturo, longevidade e fecundidade dos adultos e viabilidade desses estágios. Os resultados indicaram que as cultivares de mamão "Tainung 01", "Calimosa", "Sunrise" e "Golden" são bons hospedeiros para T. urticae. O parâmetro viabilidade não sofreu influência das cultivares em todas as fases de desenvolvimento avaliadas, apresentando valores superiores a 90%. Não houve diferença estatística entre as cultivares nos parâmetros: período de pre-oviposição e viabilidade dos ovos. A cultivar "Tainung 01" apresentou menor potencial hospedeiro, embora houvesse menor duração nos estágios de ovo, larva, protoninfa e ovo-adulto. Nos parâmetros de tabela de vida e fertilidade, apresentou menores valores de Ro, r m e λ e maior valor de Td. Entre as cultivares, o "Sunrise" apresentou um elevado potencial hospedeiro para T. urticae, pois essa cultivar proporcionou a maior produção de ovos por fêmea, maior longevidade das fêmeas, bem como a maior taxa de reprodução (maior Ro, r m e λ e menor valor de Td.The study was conducted to evaluate the Tetranichus urticae development and reproduction in commercial Carica papaya cultivars. The assayed cultivars belongs to the "Formosa" ("Tainung 01" and "Calimosa" and from the "Solo" ("Golden" and "Sunrise" groups. A fertilized female was transferred to a leaf dish (n=50 repetitions and was removed after a period of 12 hours and evaluations were done every 12 hours recording the incubation period, length of

  9. Neoseiulus fallacis (Garman) (Acari: Phytoseiidae) as a potential biological control agent for spider mites (Acari: Tetranychidae) in Virginia vineyards

    Metzger, Jessica A.

    2001-01-01

    Outbreaks of spider mites (Acari: Tetranychidae) in vineyards have been increasing with the expansion of the industry in Virginia. Only three effective acaricides are registered on grapes and as resistance begins to occur, control options are limited. Biological control of spider mites by inoculative or inundative releases of predatory mites has been tried on a wide range of crops including grapes. This project examined the feasibility of using Neoseiulus fallacis (Acari: Phytoseiidae) as ...

  10. The complete mitochondrial genome of the citrus red mite Panonychus citri (Acari: Tetranychidae): high genome rearrangement and extremely truncated tRNAs

    Dou Wei; Wang Bao-Jun; Wei Dan-Dan; Yuan Ming-Long; Wang Jin-Jun

    2010-01-01

    Abstract Background The family Tetranychidae (Chelicerata: Acari) includes ~1200 species, many of which are of agronomic importance. To date, mitochondrial genomes of only two Tetranychidae species have been sequenced, and it has been found that these two mitochondrial genomes are characterized by many unusual features in genome organization and structure such as gene order and nucleotide frequency. The scarcity of available sequence data has greatly impeded evolutionary studies in Acari (mit...

  11. New species, new records and re-description of spider mites (Acari: Tetranychidae) from India.

    Zeity, Mahran; Srinivasa, N; Gowda, C Chinnamade

    2016-01-01

    Two species of Tetranychidae (Acari), Oligonychus neotylus sp. nov. from Zea mays and Pennisetum purpureum (Poaceae) and Tetranychus hirsutus sp. nov. from Gymnema sylvestre R. Br. (Apocynaceae) are described from Karnataka state, south India. Tetranychus bambusae Wang and Ma is recorded for the first time from India and re-described. Four other species are reported for the first time from India viz., Oligonychus coniferarum (McGregor), Oligonychus duncombei Meyer, Tetranychus marianae McGregor and Tetranychus okinawanus Ehara from Cupressus sp., an undetermined grass, Centrosema pubescens and Adenium obesum, respectively. PMID:27394311

  12. Evaluation of corn plant as potential banker plant for supporting predatory gall Midge, Feltiella acarisuga (Diptera: Cecidomyiidae) against Tetranychus urticae (Acari: Tetranychidae) in greenhouse vegetable production

    The twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is one of the most important and highly polyphagous pests of vegetables and other crops worldwide. In this study, several experiments were conducted under laboratory and greenhouse conditions to evaluate whether corn plant ...

  13. The complete mitochondrial genome of the citrus red mite Panonychus citri (Acari: Tetranychidae: high genome rearrangement and extremely truncated tRNAs

    Dou Wei

    2010-10-01

    Full Text Available Abstract Background The family Tetranychidae (Chelicerata: Acari includes ~1200 species, many of which are of agronomic importance. To date, mitochondrial genomes of only two Tetranychidae species have been sequenced, and it has been found that these two mitochondrial genomes are characterized by many unusual features in genome organization and structure such as gene order and nucleotide frequency. The scarcity of available sequence data has greatly impeded evolutionary studies in Acari (mites and ticks. Information on Tetranychidae mitochondrial genomes is quite important for phylogenetic evaluation and population genetics, as well as the molecular evolution of functional genes such as acaricide-resistance genes. In this study, we sequenced the complete mitochondrial genome of Panonychus citri (Family Tetranychidae, a worldwide citrus pest, and provide a comparison to other Acari. Results The mitochondrial genome of P. citri is a typical circular molecule of 13,077 bp, and contains the complete set of 37 genes that are usually found in metazoans. This is the smallest mitochondrial genome within all sequenced Acari and other Chelicerata, primarily due to the significant size reduction of protein coding genes (PCGs, a large rRNA gene, and the A + T-rich region. The mitochondrial gene order for P. citri is the same as those for P. ulmi and Tetranychus urticae, but distinctly different from other Acari by a series of gene translocations and/or inversions. The majority of the P. citri mitochondrial genome has a high A + T content (85.28%, which is also reflected by AT-rich codons being used more frequently, but exhibits a positive GC-skew (0.03. The Acari mitochondrial nad1 exhibits a faster amino acid substitution rate than other genes, and the variation of nucleotide substitution patterns of PCGs is significantly correlated with the G + C content. Most tRNA genes of P. citri are extremely truncated and atypical (44-65, 54.1 ± 4.1 bp, lacking

  14. Beat sampling accuracy in estimating spruce spider mite (Acari: Tetranychidae) populations and injury on juniper.

    Shrewsbury, Paula M; Hardin, Mark R

    2004-08-01

    The use of a standardized beat sampling method for estimating spruce spider mite, Oligonychus ununguis (Jacobi) (Acari: Tetranychidae), densities on a widely used evergreen ornamental plant species, Juniperus chinensis variety 'Sargentii' A. Henry (Cupressaceae), was examined. There was a significant positive relationship between total spruce spider mite densities and spider mite densities from beat sampling on juniper. The slope and intercept of the relationship may be used by pest managers to predict total spider mite densities on plants from beat sample counts. Beat sampling dramatically underestimates the total number of spider mites on a foliage sample. The relationships between spruce spider mite feeding injury and spider mite density estimates from beat sampling juniper foliage and total spider mite counts on foliage were also examined. There was a significant positive relationship between spruce spider mite density as estimated from beat sampling and injury to the plants. There was a similar positive relationship between the total number of spruce spider mites and injury to the plants, suggesting that a pest manager could use beat sampling counts to estimate plant injury and related thresholds. These findings have important implications to decision-making for spruce spider mite control, especially as it relates to threshold levels and determining rates of predator releases. Further assessment of the effectiveness of beat and other sampling methods across multiple spider mite- host plant associations needs to be examined to enable pest managers to select sampling plans that are feasible and reliable. PMID:15384359

  15. Acaricidal activity of Juglans regia leaf extracts on Tetranychus viennensis and Tetranychus cinnabarinus (Acari: Tetranychidae).

    Wang, Y N; Shi, G L; Zhao, L L; Liu, S Q; Yu, T Q; Clarke, S R; Sun, J H

    2007-08-01

    Leaf extracts of the walnut, Juglans regia L., were evaluated under laboratory conditions to determine their acaricidal activity on the mites Tetranychus cinnabarinus (Boisduval) and Tetranychus viennensis Zacher (Acari: Tetranychidae). Extracts had both contact and systemic toxicity to these mites. The four solvents tested for preparing crude extracts were petroleum ether, chloroform, ethyl acetate, and methanol. Methanol was the most efficient solvent, with an extraction rate from 17.06 + 0.80 to 20.27 +/- 0.28%. Petroleum ether was the least effective solvent, with extraction rates from 2.30 +/- 0.13 to 2.71 +/- 0.13%. However, the crude extracts with petroleum ether resulted in the highest mite mortality (79.04 +/- 0.52%) in a slide dip bioassay. Mites mortalities from the concentrated extracts prepared by chloroform, ethyl acetate, methanol, or distilled water were significantly lower than petroleum ether. The mean lethal concentrations (LC50) of the extracts from petroleum ether, chloroform, ethyl acetate, methanol, and distilled water to the two mite species were 0.73 +/- 0.04, 1.66 +/- 0.28, 4.96 +/- 0.35, 7.45 +/- 0.67, and 9.91 +/- 0.32 mg/ml, respectively. After liquid chromatography and thin-layer chromatography, the concentrated extracts of petroleum ether were separated into eight fractions and tested for acaricidal activity. Fraction 6 produced significantly higher mite mortality rates than the other groups, killing approximately 90% of both species. PMID:17849883

  16. Seleções para resistência e suscetibilidade, detecção e monitoramento da resistência de Tetranychus urticae ao acaricida clorfenapir Selections for resistance and susceptibility, detection and monitoring of resistance to the acaricide chlorfenapyr in Tetranychus urticae koch (Acari: Tetranychidae)

    Mário Eidi Sato; Marcos Zatti da Silva; Katia Gigliola Cangani; Adalton Raga

    2007-01-01

    Problemas com resistência de ácaro-rajado, Tetranychus urticae Koch (Acari: Tetranychidae), a acaricidas têm sido registrados em diversos países, inclusive no Brasil. O estudo teve como objetivo caracterizar a resistência de T. urticae ao acaricida clorfenapir e avaliar a freqüência de resistência a esse composto em áreas comerciais de seis culturas no Estado de São Paulo. Seleções para resistência e suscetibilidade a clorfenapir foram realizadas em laboratório, utilizando-se uma população de...

  17. Susceptibility of Tetranychus urticae Koch. (Acari: Tetranychidae to Isolates of Entomopathogenic Fungus Beauveria bassiana

    Slavimira A. Draganova

    2010-01-01

    Full Text Available Bioassays with five isolates of the entomopathogenic fungus Beauveria bassiana (Bals.- Criv. Vuillemin were conducted under laboratory conditions with a goal to estimate their virulence to the two-spotted spider mite Tetranychus urticae Koch. (Acari: Tetranychidae. Common bean plants with mites were treated by spraying conidial suspensions of isolates at concentrations of 106, 107 and 108 conidia/ml. Lethal effects of the fungal isolates wereevaluated as percentages of cumulative daily mortality due to mycoses, corrected for mortality in the control variant. Virulence of the isolates was estimated based on values of the median lethal time (LT50 calculated by probit analysis for the variants treated with conidial suspensions at the concentration of 106 conidia/ml.The two-spotted spider mite was found susceptible to the examined isolates of the entomopathogenic fungus B. bassiana. Mycosis caused to T. urticae by the B. bassiana isolates 444 Bb and 445 Bb had fast lethal effect after treatment with conidial suspensions even at the concentration of 106 conidia/ml. The mean mortality values of host individuals were 83.78 ± 3.62% and 68.49 ± 4.28% on the first day, respectively, and up to 100% in both variants on the fourth day.The isolates 426 Bb, 444 Bb and 445 Bb of B. bassiana were highly virulent to two-spotted spider mites with values of the median lethal time varied within overlapped narrow confidence intervals from 0.122 to 1.084 days (average value 0.162 days, from 0.117 to 1.398 days (average value 0.146 days and from 0.106 to 1.162 days (average value 0.131 days,respectively. Significant differences regarding virulence of the three isolates at p-level < 0.05 could not been proved. The other two examined isolates were distinctly less virulent to T. urticae than these three B. bassiana isolates.

  18. Acaricidal and Ovicidal Effects of Sage (Salvia officinalis L.) and Rosemary (Rosmarinus officinalis L.) (Lamiaceae) Extracts on Tetranychus urticae Koch (Acari: Tetranychidae)

    Sibel YORULMAZ SALMAN; Sarıtaş, Semiha; KARA, Nimet; Ay, Recep

    2014-01-01

    Tetranychus urticae Koch (Acari: Tetranychidae) is a harmful pest for crops such as vegetables, fruits, and ornamental and industrial plants. The usage of plant extracts for pest control is seen as an alternative to synthetic pesticides. The effect of methanolic extracts obtained from sage (S. officinalis) and rosemary (R. officinalis) plants from the Lamiaceae family on T. urticae was researched in an effort to create an alternative to synthetic pesticides. The spray tower-leaf disk method w...

  19. Acaricidal and oviposition deterring effects of santalol identified in sandalwood oil against two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae)

    Roh, Hyun Sik; Lim, Eu Gene; Kim, Jinwoo; Park, Chung Gyoo

    2011-01-01

    Thirty-four plant essential oils were screened for their acaricidal and oviposition deterrent activities against two-spotted spider mite (TSSM), Tetranychus urticae Koch (Acari: Tetranychidae), in the laboratory using a leaf-dip bioassay. From initial trials, sandalwood and common thyme oils were observed to be the most effective against TSSM adult females. Subsequent trials confirmed that only sandalwood oil was significantly active (87.2 ± 2.9% mortality) against TSSM adult females. Sandalw...

  20. Controle biológico do ácaro rajado com ácaros predadores fitoseídeos (Acari: Tetranychidae, Phytoseiidae) em culturas de pepino e morango Biological control of the two-spotted spider mite (Acari: Tetranychidae, Phytoseiidae) in cucumber and strawberry crops

    Watanabe, M. A.; G.J. de Moraes; I. Gastaldo Jr.; Nicolella, G

    1994-01-01

    O ácaro rajado, Tetranychus urticae Koch, é considerado uma das principais pragas de hortaliças e várias outras culturas no Brasil, em áreas onde um considerável volume de acaricidas é anualmente empregado no seu controle. O objetivo deste trabalho foi testar a viabilidade técnica do emprego dos fitoseídeos Amblyseius idaeus (Denmark & Muma) e Phytoseudus macropilis (Banks), comum ente encontrados no Brasil, no controle de T. urticae em pepino e morangueiro em Jaguaríúna,SP. Utilizou-se o del...

  1. Controle de Brevipalpus phoenicis (Geijskes, 1939 e Oligonychus ilicis (McGregor, 1917 (Acari: Tenuipalpidae, Tetranychidae em cafeeiro e o impacto sobre ácaros benéficos: II - Spirodiclofen e Azocyclotin Control of Brevipalpus phoenicis (Geijskes, 1939 and Oligonychus ilicis (McGregor, 1917 (Acari: Tenuipalpidae, Tetranychidae in coffee plants and the impact on beneficial mites: II - Spirodiclofen and Azocyclotin

    Paulo Rebelles Reis

    2005-06-01

    Full Text Available O ácaro Brevipalpus phoenicis (Geijskes é importante em cafeeiro (Coffea spp. por ser o vetor do vírus da mancha-anular, doença responsável por queda de folhas e má qualidade da bebida do café, e o ácaro-vermelho Oligonychus ilicis (McGregor por reduzir a área foliar de fotossíntese. Ácaros da família Phytoseiidae, de várias espécies, são eficientes predadores associados aos ácaros-praga. Conduziu-se este trabalho com o objetivo de estudar o controle dos ácaros-praga com spirodiclofen e azocyclotin, e o impacto sobre fitoseídeos. Em laboratório foram estudados os efeitos ovicida, tópico, residual, tópico mais residual e a seletividade fisiológica aos fitoseídeos; em casa-de-vegetação foi avaliada a persistência no controle às duas espécies de ácaros-praga; e em campo foi avaliada a eficiência apenas no controle de B. phoenicis. Os bioensaios foram realizados em arenas de folhas destacadas. O efeito ovicida foi avaliado em ovos no início e final de incubação. Os efeitos residual, tópico e tópico mais residual foram avaliados pela mortalidade de larvas, ninfas e adultos aos oito dias, e a persistência até 30 dias após a aplicação. A seletividade aos fitoseídeos foi avaliada, pelo efeito na mortalidade e reprodução de fêmeas adultas, em teste residual em superfície de vidro. Spirodiclofen e azocyclotin (SC mostraram eficiente ação ovicida, principalmente para ovos de B. phoenicis no início de incubação. Para ovos de O. ilicis, somente o spirodiclofen apresentou efeito ovicida. Em geral, os efeitos tópico e residual associados melhoraram a eficiência dos produtos no controle das fases pós-embrionárias de ambas as espécies. O spirodiclofen apresentou seletividade aos ácaros predadores, já o azocyclotin foi nocivo. Em campo, ambos os acaricidas mostram-se altamente eficientes na redução de todas as fases pós-embrionárias do ácaro B. phoenicis, principalmente nas folhas.The mite Brevipalpus

  2. Controle biológico do ácaro rajado com ácaros predadores fitoseídeos (Acari: Tetranychidae, Phytoseiidae em culturas de pepino e morango Biological control of the two-spotted spider mite (Acari: Tetranychidae, Phytoseiidae in cucumber and strawberry crops

    M.A. Watanabe

    1994-04-01

    Full Text Available O ácaro rajado, Tetranychus urticae Koch, é considerado uma das principais pragas de hortaliças e várias outras culturas no Brasil, em áreas onde um considerável volume de acaricidas é anualmente empregado no seu controle. O objetivo deste trabalho foi testar a viabilidade técnica do emprego dos fitoseídeos Amblyseius idaeus (Denmark & Muma e Phytoseudus macropilis (Banks, comum ente encontrados no Brasil, no controle de T. urticae em pepino e morangueiro em Jaguaríúna,SP. Utilizou-se o delineamento experimental em blocos casualizados, com 4 repetições e 4 tratamentos (T1-T4 para o pepino e 5 tratamentos para o morangueiro (T1-T5: T1, testemunha; T2, pulverizações semanais de Malation para a exclusão de predadores nativos; T3, liberações periódicas de A.idaeus; T4, liberações periódicas de P.macropilis; T5, pulverizações semanais de avermectina para a exclusão de ácaros fitófagos e predadores nativos. Apenas A.idaeus se estabeleceu na cultura de pepino, reduzindo significativamente a população de T. urticae. Ambas espécies de predadores se estabeleceram na cultura do morango e reduziram significativamente a população de T.urticae.The two-spotted spider mite, Tetranychus urticae Koch, is considered one of the main pests of horticultural and other crops in Brazil, in areas where a considerable volume of acaricides is annually used for its control. The objective of this work was to test the technical viability of using phytoseiids Amblyseius idaeus (Denmark & Muma and Phytoseudus macropilis (Banks, commonly found in Brazil, to control T .urticae in cucumber and strawberry crops in Jaguaríúna,SP. A randomized complete block design was used, with 4 replicates and 4 treatments for cucumber (T1-T4 and 5 treatments for strawberry crops (T1-T5: T1, control; T2, weekly sprays of malathion for exclusion of native predators; T3, periodical releases of A.idaeus; T4, periodical releases of P. macropilis; T5, weekly sprays of

  3. The biology and fecundity of the citrus red mite Panonychus citri (McGregor) (Acari: Tetranychidae) at different temperatures under laboratory conditions

    KASAP, İsmail

    2009-01-01

    The developmental time and fecundity of Panonychus citri (McGregor 1916) (Acari: Tetranychidae) on Washington navel sweet orange (Citrus sinensis (L.) Osb.) leaves were determined at 15, 20, 25, 30 and 35 ± 1 °C, 65 ± 10% RH, and a 16:8-h (L:D) photoperiod under laboratory conditions. Total development time of P. citri females was 37.2, 16.6, 12.2, 9.8, and 9.0 days at 15, 20, 25, 30, and 35 ± 1 °C, respectively. Total and daily egg production levels of P. citri were highest at 25 °C (25.6 an...

  4. The residual and direct effects of reduced-risk and conventional miticides on twospotted spider mites, Tetranychus urticae (Acari: Tetranychidae) and predatory mites (Acari: Phytoseiidae)

    The residual effects of several reduced-risk and conventional miticides were evaluated in strawberries (Fragaria × ananassa Duchesne) on the twospotted spider mite (TSSM), Tetranychus urticae Koch (Acari: Tetranychidae) and on 2 predatory mites, Neoseiulus californicus McGregor and Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae). Experiments were conducted in the laboratory and greenhouse. The greenhouse experiments also tested the direct effects of the miticides on TSSM. The efficacy of conventional and reduced-risk miticides was evaluated on strawberry leaf discs and on whole plants for control of TSSM. Furthermore, the residual effects of these miticides were evaluated on whole strawberry plants against selective predatory mites. For TSSM, 5 treatments were evaluated: a conventional miticide; fenbutatin-oxide (Vendex[reg]) and 3 reduced-risk miticides; binfenazate (Acramite 50WP[reg]), activated garlic extract (Repel[reg]), sesame seed and castor oil (Wipeout[reg]), and a water-treated control. For predatory mites, the residual effects of only Acramite[reg] and Vendex[reg] were evaluated. Acramite[reg] was the most effective acaricide in reducing TSSM populations in both the laboratory and greenhouse experiments. Vendex[reg] and Wipeout[reg] were also effective in the laboratory, but did not cause significant reduction of TSSM in the greenhouse. Repel[reg] was the least effective of the 4 pesticides evaluated. Neither Acramite[reg] nor Vendex[reg] had a significant effect on either predatory mite species. However, there appeared to be more predatory mites on the Vendex[reg]-treated plants than on the Acramite[reg]-treated plants. There were significantly more predatory mites of both species on the cue plants, which were inoculated with TSSM versus the non-cue plants, which were not inoculated. (author)

  5. Screening of spider mites (Acari: Tetranychidae) for reproductive endosymbionts reveals links between co-infection and evolutionary history.

    Zhang, Yan-Kai; Chen, Ya-Ting; Yang, Kun; Qiao, Ge-Xia; Hong, Xiao-Yue

    2016-01-01

    Reproductive endosymbionts have been shown to have wide-ranging effects on many aspects of their hosts' biology. A first step to understanding how these endosymbionts interact with their hosts is to determine their incidences. Here, we screened for four reproductive endosymbionts (Wolbachia, Cardinium, Spiroplasma and Rickettsia) in 28 populations of spider mites (Acari: Tetranychidae) representing 12 species. Each of the four endosymbionts were identified in at least some of the tested specimens, and their infection patterns showed variations at the species-level and population-level, suggesting their distributions can be correlated with both the phylogeny and ecology of the hosts. Co-infections of unrelated bacteria, especially double infections of Wolbachia and Cardinium within the same individuals were common. Spiroplasma and Rickettsia infections were specific to particular host species, respectively. Further, the evolutionary histories of these endosymbionts were inferred by comparing the phylogenies of them and their hosts. These findings can help to clarify the interactions between endosymbionts and arthropods. PMID:27291078

  6. Transcriptome Analysis of the Carmine Spider Mite, Tetranychus cinnabarinus (Boisduval, 1867 (Acari: Tetranychidae, and Its Response to β-Sitosterol

    Chunya Bu

    2015-01-01

    Full Text Available Tetranychus cinnabarinus (Acari: Tetranychidae is a worldwide polyphagous agricultural pest that has the title of resistance champion among arthropods. We reported previously the identification of the acaricidal compound β-sitosterol from Mentha piperita and Inula japonica. However, the acaricidal mechanism of β-sitosterol is unclear. Due to the limited genetic research carried out, we de novo assembled the transcriptome of T. cinnabarinus using Illumina sequencing and conducted a differential expression analysis of control and β-sitosterol-treated mites. In total, we obtained >5.4 G high-quality bases for each sample with unprecedented sequencing depth and assembled them into 22,941 unigenes. We identified 617 xenobiotic metabolism-related genes involved in detoxification, binding, and transporting of xenobiotics. A highly expanded xenobiotic metabolic system was found in mites. T. cinnabarinus detoxification genes—including carboxyl/cholinesterase and ABC transporter class C—were upregulated after β-sitosterol treatment. Defense-related proteins, such as Toll-like receptor, legumain, and serine proteases, were also activated. Furthermore, other important genes—such as the chloride channel protein, cytochrome b, carboxypeptidase, peritrophic membrane chitin binding protein, and calphostin—may also play important roles in mites’ response to β-sitosterol. Our results demonstrate that high-throughput-omics tool facilitates identification of xenobiotic metabolism-related genes and illustration of the acaricidal mechanisms of β-sitosterol.

  7. Preliminary assays for efficiency avaliation of neem oil for control of Oligonychus yothersi (Acari: Tetranychidae) /
    Ensaios preliminares para avaliação da eficiência de óleo de neem no controle de Oligonychus yothersi (Acari: Tetranychidae)

    Renato C. Oliveira; Sandra M. J. Capelo; Amarildo Pasini

    2003-01-01

    The effect of a commercial formulation of the neem oil (azadirachtina) was evaluated in different stages of the biological cycle of the red mite of the paraguay tea Oligonychus yothersi (Acari: Tetranychidae). The formulation was shown efficient in the adults control, as well as it affected the fecundity of the females of the mite, however it didn’t inhabited oviposition, when the leaves were treated with the product.Avaliou-se o efeito de uma formulação comercial a base de neem (azadirachtin...

  8. Genetic diversity and population structure of Panonychus citri (Acari: Tetranychidae), in China based on mitochondrial COI gene sequences.

    Yuan, Ming-Long; Wei, Dan-Dan; Zhang, Kun; Gao, Yu-Zhen; Liu, Yong-Hua; Wang, Bao-Jun; Wang, Jin-Jun

    2010-12-01

    The citrus red mite, Panonychus citri (McGregor) (Acari: Tetranychidae), is regarded as one of the most important citrus pests in many countries, such as Japan, Spain, and China. In this study, the mitochondrial cytochrome oxidase subunit I gene was used to analyze genetic diversity and population structure of 15 P. citri populations collected from three citrus (Citrus spp.) planting regions of China. Our analysis found that these populations had relatively low genetic diversities. Bayesian tree and haplotype network showed that the 22 haplotypes of P. citri formed two lineages with low Bayesian posterior probabilities (0.55 and 0.63), and haplotypes in a sample were scattered throughout the Bayesian tree, indicating that there may be no significant genealogical structure among populations. However, when Yuxi and Danjiangkou populations (both without H7 haplotype) were excluded from the analysis, analysis of molecular variance found a weak, but significant, geographic structuring. Mantel test indicated a significant and positive correlation between genetic and geographical distances, reflecting certain degree of isolation by distance. The genetic differentiation based on pairwise F(ST) was not significant between most populations, and some F(ST) were even negative, indicating remarkable gene flow among these populations. The weak population structure of P. citri in this study was probably influenced by high gene flow between some populations due to long-distance dispersal of this species, which may be largely relied on the movement of plants between populations. The mismatch distribution analysis showed that no signal of population growth, but the Fu's F(S) value was significantly negative for total populations and the star-like shape of haplotype network, suggesting a history of population expansion of P. citri in China. PMID:21309245

  9. Gestión Integrada de la araña roja Tetranychus urticae Koch (Acari: Tetranychidae): optimización de su control biológico en clementinos

    Sá Argolo, Poliane

    2012-01-01

    La araña roja, Tetranychus urticae Koch (Acari: Tetranychidae) es una de las plagas más perjudiciales que afectan los huertos de clementinos en España. En los clementinos, T. urticae habita en el envés de las hojas, donde produce tela y colonias densas. Absorbe el contenido de las células, causando manchas cloróticas en el lado superior de las hojas. Al final del ernao T. urticae puede provocar en los frutos icatrices características y en consecuencia, pérdida de su valor comercial. Hasta la ...

  10. Population structure and dynamics of the cassava green mite Mononychellus tanajoa (Bondar and the predator Euseius ho (DeLeon (Acari: Tetranychidae, Phytoseiidae

    Evila C. Costa

    2012-06-01

    Full Text Available Population structure and dynamics of the cassava green mite Mononychellus tanajoa and the predator Euseius ho (Acari: Tetranychidae, Phytoseiidae. Cassava is attacked by several pests, among which the cassava green mite Mononychellus tanajoa. Predatory mites of the family Phytoseiidae are major natural enemies of pestmites and are naturally found inhabiting cassava plants in the field. We evaluated the temporal variation of the developmental stages of M. tanajoa and the most abundant predatory mite in cassava fields in the study region, the phytoseiid Euseius ho. Densities of all developmental stages of M. tanajoa were low during the rainy season, increasing over the cultivation cycle of cassava and peaking in the dry season. Overall, the larval stage of M. tanajoa presented the lowest densities throughout time. Densities of all developmental stages of E. ho were low and remained constant throughout the cultivation cycle of cassava. The number of eggs, nymphs and adults of M. tanajoa was higher in comparison to the larval stage whereas there were no differences in densities of the stages of E. ho. Densities of all developmental stages of M. tanajoa were negatively correlated with precipitation. Densities of the stages of egg, nymph and adult of M. tanajoa were positively related while the stage of larva was negatively related to temperature. We conclude that it is important to consider the population structure in studies of population dynamics of arthropods as each developmental stage experiences and responds uniquely to the local environment over time.

  11. Acaricidal activity of Annonaceae fractions against Tetranychus tumidus and Tetranychus urticae (Acari: Tetranychidae and the metabolite profile of Duguetia lanceolata (Annonaceae using GC-MS

    Dejane Santos Alves

    2015-12-01

    Full Text Available Species of the Tetranychus genus feed on plant tissues, which reduces the rate of photosynthesis and can lead to the death of plant tissues. As a result, considerable production losses are caused by these arthropods. Thus, in order to aid in the development of new products for the control of Tetranychus tumidus Banks and Tetranychus urticae Koch (Acari: Tetranychidae, the initial objective of this study was to select Annonaceae derived fractions that were soluble in dichloromethane and have acaricidal activity. Then, an exploratory analysis of the metabolite profile of the most successful fraction was performed by gas chromatography-mass spectrometry (GC-MS. Among the dichloromethane soluble fractions derived from Annona cacans Warm., Annona coriacea Mart., Annona neolaurifolia H. Rainer, Annona sylvatica A.St.-Hil., Duguetia lanceolata A.St.-Hil., Guatteria australis A.St.-Hil., Xylopia brasiliensis Spreng., Xylopia emarginata Mart. and Xylopia sericea A.St.-Hil., only the fraction from D. lanceolata stem bark reduced the survival of T. tumidus females. However, ovicidal activity was not detected when D. lanceolata stem bark was evaluated against T. tumidus eggs. Further, we studied the effect of dichloromethane soluble fractions from D. lanceolata leaves, berry fruits and stem bark on T. urticae, and the stem bark was found to be the most active fraction against T. urticae. The metabolite profile analysis of D. lanceolata stem bark by GC-MS, suggested that the main constituents were 2,4,5-trimethoxystyrene and trans-asarone.

  12. Sampling method evaluation and empirical model fitting for count data to estimate densities of Oligonychus perseae (Acari: Tetranychidae) on 'Hass' avocado leaves in southern California.

    Lara, Jesús R; Saremi, Naseem T; Castillo, Martin J; Hoddle, Mark S

    2016-04-01

    Oligonychus perseae (Acari: Tetranychidae) is an important foliar spider mite pest of 'Hass' avocados in several commercial production areas of the world. In California (USA), O. perseae densities in orchards can exceed more than 100 mites per leaf and this makes enumerative counting prohibitive for field sampling. In this study, partial enumerative mite counts along half a vein on an avocado leaf, an industry recommended practice known as the "half-vein method", was evaluated for accuracy using four data sets with a combined total of more than 485,913 motile O. perseae counted on 3849 leaves. Sampling simulations indicated that the half-vein method underestimated mite densities in a range of 15-60 %. This problem may adversely affect management of this pest in orchards and potentially compromise the results of field research requiring accurate mite density estimation. To address this limitation, four negative binomial regression models were fit to count data in an attempt to rescue the half-vein method for estimating mite densities. These models were incorporated into sampling plans and evaluated for their ability to estimate mite densities on whole leaves within 30-tree blocks of avocados. Model 3, a revised version of the original half-vein model, showed improvement in providing reliable estimates of O. perseae densities for making assessments of general leaf infestation densities across orchards in southern California. The implications of these results for customizing the revised half-vein method as a potential field sampling tool and for experimental research in avocado production in California are discussed. PMID:26861068

  13. Influence of the webbing produced by Oligonychus ilicis (McGregor) (Acari: Tetranychidae) on associated predatory phytoseiids; Influencia da teia de Oligonychus ilicis (McGregor) (Acari: Tetranychidae) sobre os fitoseideos predadores associados

    Franco, Renato A. [Bioagri Laboratorios Ltda., Piracicaba, SP (Brazil)], e-mail: r.franco@bioagri.com.br; Reis, Paulo R. [Empresa de Pesquisa Agropecuaria de Minas Gerais (EPAMIG), Belo Horizonte, MG (Brazil). Ecocentro], e-mail: paulo.rebelles@epamig.ufla.br; Zacarias, Mauricio S. [EMBRAPA Cafe, Lavras, MG (Brazil)], e-mail: zacarias@epamig.ufla.br; Oliveira, Daniel C. [Universidade Federal de Lavras (UFLA), MG (Brazil)

    2010-01-15

    Oligonychus ilicis (McGregor) is among those mite species that can cause damage to coffee plants (Coffea spp.). Species of Phytoseiidae acari are considered the most important and studied predatory mites. The objective of this study was to evaluate the effects of the webbing produced by O. ilicis on its predation by females of the phytoseiids Iphiseiodes zuluagai Denmark and Muma, Euseius citrifolius Denmark and Muma and Amblyseius herbicolus (Chant). Four bioassays were conducted, with three treatments and ten replicates. Each replicate consisted of 25 O. ilicis per experimental unit (a leaf disc of Coffea arabica) according to the tested developmental stage, in independent experiments. To spin the web, 15 adult females were put on each experimental unit for 24h; females were then removed, leaving only the web, and predators and prey to be tested were introduced. Predation was assessed after 24h. In the presence of webbing, the consumption of eggs, larvae and nymphs by I. zuluagai and eggs and larvae by E. citrifolius was lower. For A. herbicolus, egg predation was lower, but larval predation did not vary significantly and predation of nymphs and adults was higher in the presence of webbing. Predators as a whole were more efficient consuming larvae regardless of the presence of webbing. Considering the stages of O. ilicis altogether, webbing reduced the predation potential of I. zuluagai and E. citrifolius, but not of A. herbicolus. (author)

  14. Ocorrência de Brevipalpus phoenicis (Geijskes (Acari, Tenuipalpidae, Tetranychus urticae (Koch (Acari, Tetranychidae e Polyphagotarsonemus latus (Banks (Acari, Tarsonemidae sobre folhas de Ipomoea cairica (Linnaeus Sweet (Solanales, Convolvulaceae Occurrence of Brevipalpus phoenicis (Geijskes (Acari, Tenuipalpidae, Tetranychus urticae (Koch (Acari, Tetranychidae and Polyphagotarsonemus latus (Banks (Acari, Tarsonemidae on leaves of I. cairica (Linnaeus Sweet (Solanales, Convolvulaceae

    Ozana M. de A. Maia

    2006-09-01

    Full Text Available A ocorrência de três espécies acarinas fitófagas é relatada pela primeira vez sobre folhas de Ipomoea cairica. As espécies Brevipalpus phoenicis (Geijskes, Tetranychus urticae (Koch e Polyphagotarsonemus latus (Banks, foram coletadas sobre folhas de I. cairica nas imediações da Universidade Federal do Paraná, Curitiba, Paraná, Brasil, em 20 de janeiro de 2005.The first occurrence of three phytophagus mites on Ipomoea cairica, is reported. The species Brevipalpus phoenicis (Geijskes, Tetranychus urticae (Koch and Polyphagotarsonemus latus (Banks were caught on leaves of I. cairica, around Universidade Federal do Paraná, Curitiba, Paraná, Brazil, in January 20th, 2005.

  15. Influence of the webbing produced by Oligonychus ilicis (McGregor) (Acari: Tetranychidae) on associated predatory phytoseiids

    Oligonychus ilicis (McGregor) is among those mite species that can cause damage to coffee plants (Coffea spp.). Species of Phytoseiidae acari are considered the most important and studied predatory mites. The objective of this study was to evaluate the effects of the webbing produced by O. ilicis on its predation by females of the phytoseiids Iphiseiodes zuluagai Denmark and Muma, Euseius citrifolius Denmark and Muma and Amblyseius herbicolus (Chant). Four bioassays were conducted, with three treatments and ten replicates. Each replicate consisted of 25 O. ilicis per experimental unit (a leaf disc of Coffea arabica) according to the tested developmental stage, in independent experiments. To spin the web, 15 adult females were put on each experimental unit for 24h; females were then removed, leaving only the web, and predators and prey to be tested were introduced. Predation was assessed after 24h. In the presence of webbing, the consumption of eggs, larvae and nymphs by I. zuluagai and eggs and larvae by E. citrifolius was lower. For A. herbicolus, egg predation was lower, but larval predation did not vary significantly and predation of nymphs and adults was higher in the presence of webbing. Predators as a whole were more efficient consuming larvae regardless of the presence of webbing. Considering the stages of O. ilicis altogether, webbing reduced the predation potential of I. zuluagai and E. citrifolius, but not of A. herbicolus. (author)

  16. Toxicidade de natuneem sobre Tetranychus urticae Koch (Acari: Tetranychidae) e ácaros predadores da família Phytoseiidae Toxicity of the natuneem over the Tetranychus urticae Koch (Acari: Tetranychidae) and mites predators from the family Phytoseiidae

    Hilda Maria Brito; Manoel Guedes Corrêa Gondim Junior; José Vargas de Oliveira; Cláudio Augusto Gomes da Câmara

    2006-01-01

    A toxicidade de diferentes concentrações de Natuneem a Tetranychus urticae Koch e a dois ácaros predadores, Euseius alatus De Leon e Phytoseiulus macropilis (Banks), foi estudada. Foi avaliada a toxicidade sobre ovos, e o efeito residual e repelente, sobre adultos. O tratamento dos ovos consistiu na imersão em diluições de Natuneem por cinco segundos, e o efeito residual e repelente sobre adultos consistiu na imersão de discos de folhas de Canavalia ensiformes (L.) DC. também em diluições por...

  17. Biological aspects of Tetranychus marianae McGregor (Acari, Tetranychidae reared on yellow passion fruit (Passiflora edulis Sims f. flavicarpa Deg. leaves Aspectos biológicos de Tetranychus marianae McGregor (Acari, Tetranychidae sobre folhas de maracujazeiro (Passiflora edulis Sims. f. flavicarpa Deg.

    Aloyséia C. da S. Noronha

    2006-06-01

    Full Text Available The passion fruit plant is a host for several different phytophagous mites, mainly those belonging to the Tarsonemidae, Tenuipalpidae and Tetranychidae families. Among the Tetranychidae species are Tetranychus mexicanus (McGregor, 1950 and Tetranychus desertorum Banks, 1900. The occurrence of Tetranychus marianae McGregor, 1950 was detected on yellow passion fruit Passiflora edulis Sims f. flavicarpa Degener plants under field and greenhouse conditions at the headquarters of Embrapa Cassava & Fruits Tropical Research in Cruz das Almas County, State of Bahia, Northeast Brazil. Prior records of T. marianae on the passion fruit crop in Brazil as well as the biological aspects of this species are unknown. The study on the biology of T. marianae on P. edulis f. flavicarpa was carried out under controlled environmental condition of 25 ± 1ºC temperature, 80 ± 10% RH and 12 hours photophase. The egg to adult time span lasted 10.73 ± 0.18 days, with a 92% survival figure. The sexual ratio was 81% females. The mean female longevity was 24.53 days and the daily mean oviposition was 3.69 eggs/female. The intrinsic rate of increase (r m was 0.172; the finite rate of increase (l was 1.187 individuals/female/day; the mean time span of one generation (T was 22.81 days; and the net rate of reproduction (Ro was 50.14.A cultura do maracujazeiro é hospedeira de ácaros fitófagos pertencentes principalmente às famílias Tarsonemidae, Tenuipalpidae e Tetranychidae. Dentre as espécies de tetraniquídeos encontram-se Tetranychus mexicanus (McGregor, 1950 e Tetranychus desertorum Banks, 1900. A ocorrência de Tetranychus marianae McGregor, 1950 foi verificada em plantas de maracujá amarelo Passiflora edulis Sims. f. flavicarpa Degener, em condições de campo e casa de vegetação, na sede da Embrapa Mandioca e Fruticultura Tropical, em Cruz das Almas, BA. O registro de T. marianae na cultura do maracujá no Brasil, assim como os aspectos biológicos dessa esp

  18. Efeitos do nim sobre tetranychus urticae Koch (Acari: Tetranychidae) e os predadores Phytoseiulus macropilis (Banks) e Neoseiulus californicus (Mcgregor) (Acari: Phytoseiidae)

    Daniele Cristine Hoffmann Schlesener; Adriane Fonseca Duarte; Milton Fernando Cabezas Guerrero; Uemerson Silva da Cunha; Dori Edson Nava

    2013-01-01

    O objetivo deste trabalho foi avaliar a eficiência de controle e os efeitos adversos de dois produtos à base de nim Azamax® (Azadiractina A/B 12g/L) e Neemseto® (Azadiractina A/B, Nimbina e Salanina 2,389 g/L) sobre o ácaro-rajado Tetranychus urticae e os predadores Phytoseiulus macropilis e Neoseiulus californicus em laboratório. Para o ácaro-rajado, foram consideradas as variáveis mortalidade, fecundidade, efeito ovicida e persistência biológica, enquanto para os fitoseídeos consideraram-se...

  19. The genus Paraplonobia Wainstein and Neopetrobia Wainstein (Acari, Trombidiformes, Tetranychidae) from Saudi Arabia: new species, new records and key to the world species of Paraplonobia

    Kamran, Muhammad; Mirza, Jawwad Hassan; Alatawi,Fahad Jabr

    2016-01-01

    The two tetranychid genera Paraplonobia Wainstein and Neopetrobia Wainstein (Trombidiformes: Tetranychidae) are reported for the first time from Saudi Arabia. Three new species Paraplonobia (Anaplonobia) arabica Mirza & Alatawi, sp. n., P. (A.) haloxylonia Alatawi & Mirza, sp. n. and P. (A.) tabukensis Kamran & Alatawi, sp. n. are described and illustrated based on adult females, collected from Prosopis juliflora (SW.) Dc. (Fabaceae) and Haloxylon salicornicum Bunge (Amaranthaceae...

  20. Sensibilité à Mononychellus tanajoa Bondar (Acari : Tetranychidae de quelques cultivars de manioc (Manihot esculenta Crantz et incidence des attaques sur le rendement, dans la région des hauts plateaux de l'Ouest Cameroun

    Badegana, AM.

    2001-01-01

    Full Text Available Sensibility to Mononychellus tanajoa Bondar (Acari : Tetranychidae of some Cassava (Manihot esculenta Crantz, Cultivars and Effect of Damage on Yield Loss in the Cameroonian Western High-lands. The study of the sensibility towards the green mite Mononychellus tanajoa Bondar of some cassava (Manihot esculenta Crantz cultivars and the assessment of the effect of damage on the yield losses was carried out in the Western highlands of Cameroon. Four cassava cultivars were used : two local (Dschang and Njombe and two improved varieties (IITA 8017 and IITA 82516. The results obtained showed that the density (mites number/cm2 of leaf area was low during the rainy season and high during the dry season, which means that rains reduce the mite population by washing or lead the mites to death. The highest mean density (3.40 mites/cm2 of leaf area was obtained on the local Njombe cultivar which is consequently the most sensitive. On the contrary, the local Dschang cultivar, with the lowest mite density (1.40 mites/cm2 of leaf area was the most resistant followed by the IITA 8017 cultivar (1.74 mites/cm2. The IITA82516 cultivar had a mean density of2.65 mites/cm2. Yield losses ranged from 36.90 % for IITA 8017 (1.74 mites/cm2 and damages level of 2.75 to 58.70 % for local Dschang (3.40 mites/cm2 and damages level of 3.84. Local Dschang cultivar (1.40 mites/cm2 and damage level of 2.96 and IITA 82516 (2.65 mites/cm2 and damage level of 2.96 had a yield losse of38.10 % and 41.80 %. The results showed that higher the mite density and damage level are, higher is the yield loss, unless the cultivar is tolerant.

  1. Influence de l'usage préventif des pesticides sur les acariens Tetranychus urticae et Phytoseiulus persimilis (Acari : Tetranychidae, Phytoseiidae présents en cultures de fraisiers du Nord du Maroc

    Lagziri, M.

    2015-01-01

    Full Text Available Influence of previous pesticide use on Tetranychus urticae and Phytoseiulus persimilis (Acari: Tetranychidae, Phytoseiidae from strawberry crops in the north of Morocco. Description of the subject. Effects of recommended doses of five common pesticides in the strawberries of Loukkos area (Morocco were tested on the two-spotted spider mite Tetranychus urticae and its predatory mite Phytoseiulus persimilis. Laboratory study assessed the contact toxicity of one avermectin miticide (abamectin, two pyrethrinoid insecticide-acaricides (bifenthrin and lambda-cyalothrin, and two fungicides: firstly, mancozeb, which belongs to the dithiocarbamates family of chemicals and secondly, hexaconazole, of the triazole family. Objectives. The aim of the present study was to test in laboratory conditions the effect of five pesticides on the two-spotted spider mite, T. urticae and its predatory mite, P. persimilis. The five pesticides tested were those most frequently applied for at least two years on strawberries at the experimental site. Method. Bioassays were performed with populations of mites originating from different plots with various crop protection backgrounds. The first group of plots had been repeatedly treated with the five tested pesticides during a two-year period, the second group had been moderately treated, and the third had been treated once with the tested pesticides. Results. Our results showed that the tested pesticides provided effective control of T. urticae but that they were not compatible with use on the predatory mite P. persimilis, as these particular mites did not usually come into contact with these products. On the other hand, in plots where pesticides had been used for a long time, the susceptibility of P. persimilis populations to these products was significantly reduced. Conclusions. If the tested pesticides are to be considered for integrated pest control programs in plots where they have been used for a long time and where P

  2. Seleções para resistência e suscetibilidade, detecção e monitoramento da resistência de Tetranychus urticae ao acaricida clorfenapir Selections for resistance and susceptibility, detection and monitoring of resistance to the acaricide chlorfenapyr in Tetranychus urticae koch (Acari: Tetranychidae

    Mário Eidi Sato

    2007-01-01

    Full Text Available Problemas com resistência de ácaro-rajado, Tetranychus urticae Koch (Acari: Tetranychidae, a acaricidas têm sido registrados em diversos países, inclusive no Brasil. O estudo teve como objetivo caracterizar a resistência de T. urticae ao acaricida clorfenapir e avaliar a freqüência de resistência a esse composto em áreas comerciais de seis culturas no Estado de São Paulo. Seleções para resistência e suscetibilidade a clorfenapir foram realizadas em laboratório, utilizando-se uma população de T. urticae coletada em 2002 de um cultivo comercial de crisântemo em Holambra (SP. Após seis seleções para resistência e cinco seleções para suscetibilidade, foram obtidas as linhagens suscetível (S e resistente (R de T. urticae a clorfenapir. A razão de resistência (CL50 R/ CL50 S obtida alcançou valores de 571 vezes. Estabeleceu-se uma concentração discriminatória de 37,4 mg L-1 de ingrediente ativo (i.a. para o monitoramento da resistência de T. urticae a clorfenapir. O monitoramento foi realizado coletando-se 21 populações de ácaros em áreas comerciais de diferentes culturas (mamão, morango, feijão, tomate, crisântemo, rosa, em vários municípios do Estado de São Paulo. Arenas confeccionadas com folha de feijão foram infestadas com ácaros T. urticae e pulverizadas com clorfenapir, na sua concentração discriminatória, em torre de Potter. Os resultados indicaram grande variabilidade entre as populações com relação à suscetibilidade a clorfenapir. Foram observadas populações com freqüências de resistência entre 0,0 e 65,4%. As maiores freqüências de resistência foram observadas para populações coletadas de crisântemo em Holambra (SP.Problems associated with acaricide resistance in Tetranychus urticae Koch have been recorded in several countries including Brazil. The objective of this study was to characterize the resistance of T. urticae to the acaricide chlorfenapyr and to evaluate the resistance

  3. Efeitos do nim sobre tetranychus urticae Koch (Acari: Tetranychidae) e os predadores Phytoseiulus macropilis (Banks) e Neoseiulus californicus (Mcgregor) (Acari: Phytoseiidae) Effects of neem on tetranychus urticae Koch (Acari: Tetranychidae) and the predators Phytoseiulus macropilis (Banks) and Neoseiulus Californicus (Mcgregor) (Acari: phytoseiidae)

    Daniele Cristine Hoffmann Schlesener; Adriane Fonseca Duarte; Milton Fernando Cabezas Guerrero; Uemerson Silva da Cunha; Dori Edson Nava

    2013-01-01

    O objetivo deste trabalho foi avaliar a eficiência de controle e os efeitos adversos de dois produtos à base de nim Azamax® (Azadiractina A/B 12g/L) e Neemseto® (Azadiractina A/B, Nimbina e Salanina 2,389 g/L) sobre o ácaro-rajado Tetranychus urticae e os predadores Phytoseiulus macropilis e Neoseiulus californicus em laboratório. Para o ácaro-rajado, foram consideradas as variáveis mortalidade, fecundidade, efeito ovicida e persistência biológica, enquanto para os fitoseídeos consideraram-se...

  4. Efeitos do nim sobre tetranychus urticae Koch (Acari: Tetranychidae e os predadores Phytoseiulus macropilis (Banks e Neoseiulus californicus (Mcgregor (Acari: Phytoseiidae Effects of neem on tetranychus urticae Koch (Acari: Tetranychidae and the predators Phytoseiulus macropilis (Banks and Neoseiulus Californicus (Mcgregor (Acari: phytoseiidae

    Daniele Cristine Hoffmann Schlesener

    2013-03-01

    Full Text Available O objetivo deste trabalho foi avaliar a eficiência de controle e os efeitos adversos de dois produtos à base de nim Azamax® (Azadiractina A/B 12g/L e Neemseto® (Azadiractina A/B, Nimbina e Salanina 2,389 g/L sobre o ácaro-rajado Tetranychus urticae e os predadores Phytoseiulus macropilis e Neoseiulus californicus em laboratório. Para o ácaro-rajado, foram consideradas as variáveis mortalidade, fecundidade, efeito ovicida e persistência biológica, enquanto para os fitoseídeos consideraram-se mortalidade e fecundidade. A mortalidade máxima observada para o ácaro-rajado foi de 89,7% e 91,5% para Azamax® e Neemseto®, respectivamente, na concentração de 0,5% após a reaplicação do produto no sétimo dia. Também foram observados efeitos adversos sobre a fecundidade e a viabilidade dos ovos quando tratados com os produtos comerciais (p.c.. A persistência biológica dos produtos foi de aproximadamente três dias após a pulverização. As formulações apresentaram seletividade em relação aos fitoseídeos, porém causaram redução da fecundidade dos mesmos.The aim of the present study was to evaluate the efficiency of control and adverse effects of two neem based products: Azamax TM (Azadirachtin A/B 12g/L and Neemseto TM (Azadirachtin A/B, Nimbin and Salanin 2,389 g/L over two-spotted spider mite Tetranychus urticae and the predators Phytoseiulus macropilis and Neoseiulus californicus in laboratory. Effects over the mortality, fecundity, eggs and biological persistence of the two-spotted spider mite when treated with neem based products were evaluated. For the phytoseiids the effects on mortality and fecundity were evaluated. The maximum mortality of two-spotted spider mites rates observed were 89.7% and 91.5% for Azamax TM and Neemseto TM respectively, on the 0.5% concentration after reapplying the product on the seventh day. Adverse effects were also observed over the fecundity and viability of the eggs when treated with

  5. Eupalopsellus prasadi Bagheri and Khanjani 2009 (Acari: Prostigmata, Eupalopsellidae), a New record for the Turkish acaro fauna

    KASAP, İsmail; Çobanoğlu, Sultan; PEHLİVAN, SERKAN; KÖK, Şahin

    2013-01-01

    Females of Eupalopsellus prasadi Bagheri and Khanjani 2009, (Acari: Eupalopsellidae) were collected from the colony of Tetranychus urticae Koch (Acari: Tetranychidae) which are harmful on grown apple trees (Malus domestica Borkh. (Rosaceae)) in Bigalı village at Eceabat, Çanakkale. This is a new record for the Turkish fauna.

  6. A comparative study of the dynamics of Wolbachia infection in different populations of Tetranychus urticae (Acari : Tetranychidae)%共生菌Wolbachia在中国二斑叶螨种群中的扩散规律

    谢蓉蓉; 陈小琳; 孙荆涛; 洪晓月

    2013-01-01

    共生菌Wolbachia在中国二斑叶螨Tetranychus urticae Koch中分布广泛,所有的地理种群中均感染Wolbachia.以二斑叶螨湖南长沙(HN),辽宁兴城(LN)和江苏徐州(JS)3个地理种群为实验材料,经筛选获得100%感染和不感染Wolbachia的品系后,人工设置Wolbachia感染率为50%的品系,通过PCR技术检测二斑叶螨连续世代Wolbachia感染率动态变化,研究Wolbachia在二斑叶螨种群中的扩散规律.结果表明:3个地理种群的垂直传播效率都为100%;HN种群Wolbachia感染率上升速度最快,F7代达到100%感染;LN种群F12达到100%感染;而JS地理种群中Wolbachia感染率速度上升最慢,在F20代达到100%感染,其后感染率均能稳定在100%.LN种群Wolbachia通过诱导胞质不亲和的策略,JS种群的Wolbachia通过提高寄主适合度的策略,而HN种群Wolbachia则通过诱导胞质不亲和与提高寄主适合度两者相结合的策略,最终达到在二斑叶螨中维持感染状态并扩散传播的目的.本研究结果为今后利用Wolbachia的扩散规律控制有害生物及疾病传播提供了基础.%Maternally inherited Wolbachia bacteria are widely distributed in Chinese populations of the two-spotted spider mite Tetranychus urticae Koch (Acari; Tetranychidae). All populations were found to be infected with Wolbachia. Using the Hunan ( HN) , Liaoning ( LN ) and Jiangsu ( JS) populations of T. urticae as experimental subjects, 100% Wolbachia-infected and 100% uninfected spider mite lines were obtained by screening. This study investigated the dynamics of Wolbachia spread in the two-spotted spider mite. Infection frequencies of Wolbachia among the progenies of the artificial populations, initiated with 50% infected and 50% uninfected female adults, were monitored by PCR. The results show 100% maternal transmission in all three populations. The rate of spread was fastest in the HN population in which the infection rate reached 100% by the F7

  7. Standardization of a rearing procedure of Tetranychus urticae Koch (Acari: Tetranychidae) on bean (Phaseolus vulgaris): plant age and harvest time; Padronizacao da criacao de Tetranychus urticae Koch (Acari: Tetranychidae) em feijoeiro (Phaseolus vulgaris): idade da planta e tempo de colheita

    Bustos, Alexander; Cantor, Fernando; Cure, Jose R; Rodriguez, Daniel [Universidade Militar Nueva Granada, Bogota (Colombia). Facutad de Ciencias. Programa de Biologia Aplicada], e-mail: fernando.cantor@unimilitar.edu.co

    2009-09-15

    A rearing technique was standardized to produce Tetranychus urticae Koch on Phaseolus vulgaris (ICA Cerinza variety) as a prey of the predatory mite Phytoseiulus persimilis Athias-Henriot. Two assays were conducted to assess the following variables: the most suitable plant age for mite infestation, and the best time to harvest the mites and re infest the plants. In the first experiment, four, five, six, and seven-week-old plants of P. vulgaris were infested with six T. urticae per foliole. The lower plant stratum exhibited the largest number of mites regardless of plant age. However, four-week old plants had the larger average number of individuals. In the second experiment four-week-old plants were infested with 0.5 female mite/cm{sup 2} of leaf. The number of individuals per instar of T. urticae was recorded weekly. The highest mite production occurred between four and five weeks after infestation, indicating this to be the most suitable for mite harvesting and for plant reinfestation. (author)

  8. Can Euseius alatus DeLeon (Acari: Phytoseiidae) prey on Aceria guerreronis Keifer (Acari: Eriophyidae) in coconut palm?; Pode Euseius alatus DeLeon (Acari: Phytoseiidae) predar Aceria guerreronis Keifer (Acari: Eriophyidae) em coqueiro?

    Melo, Jose W. da S.; Domingos, Cleiton A.; Gondim Junior, Manoel G.C. [Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE (Brazil). Dept. de Agronomia. Area de Fitossanidade]. E-mail: mguedes@depa.ufrpe.br; Moraes, Gilberto J. de [Universidade de Sao Paulo (USP), Piracicaba, SP (Brazil). Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Dept. de Entomologia, Fitopatologia e Zoologia Agricola]. E-mail: gjmoraes@carpa.ciagri.usp.br

    2009-01-15

    Mites of the genus Euseius are generally considered specialist as pollen feeders. Euseius alatus DeLeon is one of the six species of phytoseiid mites most commonly found on coconut plants in northeast Brazil associated with Aceria guerreronis Keifer. Although the morphology of E. alatus does not favor the exploitation of the meristematic area of the fruit inhabited by A. guerreronis, the predator may have some role in the control of this eriophyid during the dispersion process. The objective of this work was to evaluate the development and reproduction of E. alatus on the following diets: A. guerreronis, Ricinus communis pollen (Euphorbiaceae), and Tetranychus urticae Koch (Tetranychidae) + R. communis pollen + honey solution 10%. Euseius alatus developed slightly faster and had slightly higher oviposition rate when feeding on the diet composed of T. urticae + pollen + honey. However, life table parameters were very similar on all diets, suggesting that E. alatus may contribute in reducing the population of A. guerreronis in the field. (author)

  9. Experimental infestation with the immatures of Amblyomma dissimile Koch, 1844 (Acari: Ixodidae on Tropidurus torquatus (Lacertilia: Iguanidae and Oryctolagus cuniculus Infestação experimental com as fases imaturas de Amblyomma dissimile Koch, 1844 (Acari: Ixodidae em Tropidurus torquatus (Lacertilia: Iguanidae e Oryctolagus cuniculus

    L.H.T. Freitas

    2004-02-01

    Full Text Available Larvas provenientes de duas fêmeas de Amblyomma dissimile Koch, 1844 (Acari: Ixodidae, naturalmente ingurgitadas em uma iguana (Iguana iguana e provenientes do Estado do Mato Grosso, foram utilizadas na infestação experimental de lagartos da espécie Tropidurus torquatus e coelhos domésticos. As larvas alimentadas em ambos os hospedeiros realizaram ecdise para ninfas. As ninfas apenas ingurgitaram no lagarto e mudaram para machos e fêmeas. Este é o primeiro registro do parasitismo de larvas e ninfas de A. dissimile em T. torquatus e de larvas em coelhos.

  10. 二斑叶螨两种群中Wolbachia诱导的胞质不亲和作用的影响因子比较研究%A comparative study of factors influencing the expression of Wolbachiainduced cytoplasmic incompatibility in two populations of the two-spotted spider mite, Tetranychus urticae Koch ( Acari: Tetranychidae)

    陆明红; 谢蓉蓉; 赵臻君; 于明志; 薛晓峰; 洪晓月

    2011-01-01

    The cytoplasmic incompatibility (CI) is the most common effect of Wolbachia on the reproduction of its arthropod hosts, and the expression of CI differs greatly among different populations. Using the Jiangsu (JS) and Liaoning (LN) populations of the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae) , as experimental materials, 100% infected and uninfected Wolbachia lines were obtained by screening. The present study tried to evaluate some factors influencing the expression of CI in the spider mite by crossing experiment and Real-time quantitative PCR. These factors include age of host, temperature, host genes and Wolbachia density. The 1, 3, 5, 7-day-old virgin males were used to investigate the influence of host age on Wolbachia-induced CI. The results showed no effect of age on CI, suggesting that host age does not reduce the sperm modification induced by Wolbachia. The effect of temperatures (20℃, 25℃ and 30℃ ) on the CI induced by Wolbachia was also checked. Neither high nor low temperatures influenced the expression of CI. Wolbachia density in males of the JS population, as measured by quantitative PCR using the wsp (surface protein of Wolbachia) gene, was significantly higher than that in the LN population. In addition, in both the JS and LN populations, Wolbachia density increased with the age of male hosts. Wolbachia density also showed no effect on CI. We estimated the variability of CI expression between the JS and LN population of T. Urticae was due to the interaction between Wolbachia and host genotypes. The results might provide foundation for understanding the mechanisms of reproductive manipulation induced by Wolbachia.%Wolbachia诱导胞质不亲和(cytoplasmic incompatibility,CI)是对寄主的生殖调控中最常见的一种方式,在不同种群中CI表达的差异较大.以二斑叶螨Tetranychus urticae辽宁兴城(LN)和江苏徐州(JS)两个地理种群为实验材料,经筛选获得100%感染Wolbachia和不感

  11. Interactions in a tritrophic acarine predator-prey metapopulation system V: Within-plant dynamics of <em>Phytoseiulus persimilisem> and <em>Tetranychus urticaeem> (Acari: Phytoseiidae, Tetranychidae)

    Nachman, Gösta; Zemek, Rostislav

    2003-01-01

    Biological control, Bottom-up factor, Phytoseiulus persimilis, Plant condition, Predacious mites, Simulation model, Tetranychus urticae, Top-down factor, Two-spotted spider mites......Biological control, Bottom-up factor, Phytoseiulus persimilis, Plant condition, Predacious mites, Simulation model, Tetranychus urticae, Top-down factor, Two-spotted spider mites...

  12. Ocorrência do ácaro fitófago Catarhinus tricholaenae Keifer (Acari: Diptilomiopidae em cultivares de milho Bt Occurrence of the phytophagous mite Catarhinus tricholaenae Keifer (Acari: Diptilomiopidae on Bt corn cultivars

    Marcos Antônio Matiello Fadini

    2012-09-01

    Full Text Available O objetivo deste trabalho foi avaliar a ocorrência do microácaro-da-face-inferior-das-folhas-de-milho Catarhinus tricholaenae Keifer (Acari: Diptilomiopidae em cultivares transgênicas de milho, contendo as proteínas Cry1F e Cry 1 A(b e milho não Bt. Durante o período de junho de 2010 a janeiro de 2011, foram coletadas, quinzenalmente, cinco amostras aleatórias de quatro folhas em talhões de milho Bt, contendo a proteína Cry 1F e Cry 1 A(b, e de milho não Bt em áreas experimentais da Embrapa Milho e Sorgo, em Sete Lagoas, MG. As amostras de folhas foram vistoriadas por 15 minutos na região da nervura central, em busca de adultos de C. tricholaenae. Foram registrados 2.930 indivíduos de C. tricholaenae, sendo que 1.114 no milho Bt Cry 1F, 753 em Cry 1 A(b e 1063 indivíduos em folhas das cultivares não Bt. As maiores abundâncias populacionais médias ocorreram nos meses de novembro e dezembro. Os fatores estágio fenológico das plantas e precipitação afetaram positivamente a abundância de C. tricholaenae. A abundância média do período de coleta de C. tricholaenae foi reduzida pela cultivar de milho contendo a proteína Cry 1 A(b. Esse é o primeiro registro de ácaros sobre cultivares de milho transgênico no Brasil.The objective of this study was to evaluate the occurrence of "microácaro-da-face-inferior-das-folhas-de-milho" Catarhinus tricholaenae Keifer (Acari: Diptilomiopidae on transgenic cultivars of corn containing proteins Cry1F and Cry 1 A (b and non-Bt corn. During the period from June 2010 to January 2011 were collected, every two weeks, five random samples of four leaves in plots of Bt corn containing the protein Cry 1F and Cry 1 A (b and non-Bt corn in the experimental area of Embrapa Corn and Sorghum, Sete Lagoas, MG. The leaf samples were examined for 15 minutes in the central region of leaf in search of adult C. tricholaenae. We recorded 2930 individuals of C. tricholaenae, 1114 on Bt Cry 1F, 752 on Cry 1 A

  13. Experimental infestation with the immatures of Amblyomma dissimile Koch, 1844 (Acari: Ixodidae) on Tropidurus torquatus (Lacertilia: Iguanidae) and Oryctolagus cuniculus Infestação experimental com as fases imaturas de Amblyomma dissimile Koch, 1844 (Acari: Ixodidae) em Tropidurus torquatus (Lacertilia: Iguanidae) e Oryctolagus cuniculus

    L.H.T. Freitas; Faccini, J. L. H.; E DAEMON; M.C.A. Prata; D.M. Barros-Battesti

    2004-01-01

    Larvas provenientes de duas fêmeas de Amblyomma dissimile Koch, 1844 (Acari: Ixodidae), naturalmente ingurgitadas em uma iguana (Iguana iguana) e provenientes do Estado do Mato Grosso, foram utilizadas na infestação experimental de lagartos da espécie Tropidurus torquatus e coelhos domésticos. As larvas alimentadas em ambos os hospedeiros realizaram ecdise para ninfas. As ninfas apenas ingurgitaram no lagarto e mudaram para machos e fêmeas. Este é o primeiro registro do parasitismo de larvas ...

  14. Biological studies of Oligonychus punicae (Acari: Tetranychidae) on grapevine cultivars.

    Vásquez, Carlos; Aponte, Orlando; Morales, José; Sanabria, María E; García, Grisaly

    2008-06-01

    Life cycle, fecundity and longevity of the avocado brown mite, Oligonychus punicae (Hirst), were studied on six grapevine cultivars (Tucupita, Villanueva, Red Globe, Sirah, Sauvignon and Chenin Blanc), under laboratory conditions at 27 +/- 2 degrees C, 80 +/- 10% RH, and L12:D12 photoperiod. Mite-infested leaves were collected from vineyards, placed in paper bags and taken to the laboratory. A laboratory mite culture was established using the grape cultivar Criolla Negra as host plant. To elucidate potential effects on avocado brown mite parameters, we assessed levels of secondary metabolites, such as alkaloids, flavonoids, tannins and polyphenols, of leaves of the six grape cultivars, as well as the thickness of the adaxial cuticle-epidermis. The life cycle of O. punicae differed among cultivars with average values ranging between 8.2 days on Tucupita leaves and 9.1 days on Sirah. Relatively high fecundity was found on Tucupita leaves (2.8 eggs/female/day) during 11.4 oviposition days, while low fecundity values occurred on Sirah and Villanueva leaves, with 0.9 and 1.8 eggs/female/day during 7.9 and 6.7 days, respectively. Average longevity of O. punicae females ranged from 8.1 to 17.5 days on Sirah and Sauvignon leaves, respectively. Intrinsic rate of increase (r (m)) was highest on Sauvignon (0.292) and Tucupita (0.261), and lowest on Sirah (0.146) and Villanueva (0.135). Although significant differences in cuticle-epidermis thickness were detected among the six cultivars, it seemed not to affect mite parameters. Secondary metabolite content also varied between the cultivars. Generally, increasing flavonoid content coincided with decreasing reproductive parameters. The natural plant resistance observed in this study could be useful in the development of an integrated pest management program for mite pests in grape production. PMID:18483791

  15. Comparação econômica entre controle biológico e químico para o manejo de ácaro-vermelho em macieira Economic comparison of biological and chemical control in the management of red spider mites in apple orchard

    Lino Bittencourt Monteiro

    2006-12-01

    Full Text Available O ácaro-vermelho da macieira, Panonychus ulmi (Acari: Tetranychidae, é uma importante praga na cultura da macieira em Fraiburgo - SC, e o controle biológico aplicado foi implantado em meados dos anos 90. O objetivo deste trabalho foi demonstrar os benefícios econômicos da utilização do controle biológico no manejo do ácaro-vermelho. A avaliação foi realizada em dois pomares comerciais de macieiras. Em um deles, foi implantado o controle biológico aplicado de ácaros, baseado na liberação do ácaro predador Neoseiulus californicus (Acari: Phytoseiidae, seleção de inseticidas e manejo de ervas invasoras, e o outro pomar seguiu o manejo convencional de artrópodes, baseado na aplicação de produtos químicos para o controle de insetos, ácaros fitófagos e ervas invasoras. A análise econômica mostrou que os custos com mão-de-obra e máquinas foram semelhantes em ambos os pomares, entretanto os custos com acaricidas foram significativamente inferiores no pomar onde o manejo foi o controle biológico, demonstrando que, apesar da necessidade de investimentos em instalações para a criação do ácaro predador e custos de manutenção das mesmas, a estratégia biológica foi economicamente viável.Red spider mite, Panonychus ulmi (Acari: Tetranychidae is a significant pest in apple tree in Fraiburgo, Santa Catarina (SC and applied biological control was implemented in the mid-nineties. The objective of this study was to demonstrate the economic benefits of biological control in the management of red spider mite. The assessment was carried out in two commercial orchards, one of which was subjected to biological control of spider mite by releasing the predator Neoseiulus californicus, insecticide selection and weed management, and the other to conventional arthropod management, based on the application of chemicals to control insects, phytofagous mites and weed. Economic analysis showed that the costs for labor and machines were

  16. Mortalidade do ácaro predador Neoseiulus californicus (Acari: Phytoseiidae em testes de toxicidade residual de inseticidas e acaricidas usuais em pomáceas Mortality of predatory mite Neoseiulus californicus (Acari: Phytoseiidae in residual toxicity persistence tests

    Marcelo Gustavo Ruiz

    2008-12-01

    Full Text Available Os ácaros fitoseídeos, especialmente Neoseiulus californicus (McGregor, são importantes agentes de controle biológico de ácaros tetraniquídeos-praga nas culturas de pomáceas no "Alto Valle del Río Negro y Neuquén", Argentina. Neste trabalho, avaliou-se a mortalidade de N. californicus quando exposto a resíduos dos inseticidas azimphos-methyl, carbaryl e cyfluthrin, e dos acaricidas cyhexatin e propargite. Os produtos foram aplicados às concentrações recomendadas em plantas de pereira. Um, três, seis e dez dias após a aplicação (DAA, folhas tratadas foram retiradas das plantas para a preparação de unidades experimentais. Cinco adultos de N. californicus, provenientes de criação-estoque, foram transferidos para cada unidade, onde pólen de taboa foi fornecido como alimento. As unidades foram mantidas a 25 ± 2 ºC, 60 ± 10% de umidade relativa e fotoperíodo de 14 h. A mortalidade do ácaro foi avaliada 24 h após o confinamento. As médias de mortalidade foram comparadas pelo teste de Dunnett, a 5% de probabilidade. A progressão do declínio do efeito dos produtos testados foi submetida à análise de regressão. Nas duas primeiras datas de avaliação, todos os produtos apresentaram valores de mortalidade significativamente diferentes da testemunha tratada com água. Seis dias após a aplicação, propargite, cyhexatin e cyfluthrin apresentaram mortalidade de aproximadamente 30%, enquanto a mortalidade nos tratamentos azimphos-methyl e carbaryl apresentou níveis estatisticamente similares aos da testemunha. Dez dias após a aplicação, a mortalidade em todos os tratamentos não diferiu significativamente da testemunha. O efeito de todos os produtos apresentou declínio progressivo ao longo do período de observação, sendo significativa a 1% de probabilidade a regressão linear negativa para os valores obtidos. Os maiores efeitos negativos sobre a sobrevivência de N. californicus corresponderam aos acaricidas testados

  17. Distribuição espacial e plano de amostragem de Calacarus heveae (Acari em seringueira Spatial distribution and sampling plan for Calacarus heveae (Acari on rubber trees

    Noeli Juarez Ferla

    2007-12-01

    Full Text Available Calacarus heveae Feres, 1992 é um eriofídeo descrito de espécimes coletados em plantas de seringueira (Hevea brasiliensis, Euphorbiaceae na região noroeste do Estado de São Paulo. Esse ácaro prefere a face adaxial dos folíolos e pode causar a perda do brilho, amarelecimento, bronzeamento dessa região e a subseqüente queda prematura das folhas. O objetivo deste trabalho foi analisar a distribuição de C. heveae em seringueira, selecionar a unidade de amostragem mais representativa e desenvolver um plano de amostragem para o estudo de sua flutuação populacional. O trabalho foi conduzido com os clones PB 260 e IAN 873, respectivamente nos municípios de Itiquira e de Pontes e Lacerda, ambos no Mato Grosso. Em Itiquira, diferenças significativas foram observadas em quatro ocasiões em relação ao número médio de ácaros por folha nos diferentes estratos das plantas. Nas amostragens realizadas em Pontes e Lacerda, nenhuma diferença significativa foi encontrada entre os estratos em relação àquele parâmetro. Apenas em Itiquira, em uma ocasião de amostragem, foi verificada diferença entre os três estratos, em relação à proporção de folhas infestadas. Nenhuma diferença significativa foi verificada em relação ao número médio de ácaros por folha e proporção de folhas infestadas por C. heveae a diferentes distâncias da periferia da copa. Calacarus heveae exibe distribuição agregada no campo. Para estimar a densidade de C. heveae, um plano numérico e um plano binomial de amostragem foram desenvolvidos.Calacarus heveae Feres, 1992 (Eriophyidae is a mite described from specimens collected on rubber tree (Hevea brasiliensis, Euphorbiaceae in the northwest region of the state of São Paulo. This mite prefers the adaxial face of the folioles which it can turn dry, yellowish and brownish; it can also cause leaf fall. The aim of this work was to analyze the distribution of C. heveae on rubber trees, to select the most

  18. Environmentally associated ticks (Acari: Ixodidae in Campo Grande, Mato Grosso do Sul, Brazil Carrapatos (Acari: Ixodidae associados com o ambiente em Campo Grande, Mato Grosso do Sul, Brasil

    Marcos Valério Garcia

    2013-03-01

    Full Text Available Herein, we report tick species found on wild and domestic animals and in the environment during a one-year sampling period at the Brazilian Farming Research Company beef cattle unit (Embrapa Beef Cattle, which is located within the urban area of Campo Grande, Mato Grosso do Sul, Brazil. From 55 wild hosts including six different species (Nasua nasua, Cebus spp., Cerdocyon thous, Myrmecophaga tridactyla, Tamandua tetradactyla and Dasyprocta aguti, 323 ticks were collected. Amblyomma ovale ticks were found solely on coatis, and Amblyomma nodosum was identified solely on anteaters. No ticks were found on capuchin monkeys. However, Amblyomma cajennense was found on all parasitized host species with the exception of capuchin monkeys. Giant anteaters displayed the highest infestation abundance, with a mean of 53 ticks∕animal. Environmental sampling yielded 166 adult A. cajennense ticks. The tick species found on domestic animals (Rhipicephalus (Boophilus microplus, R. sanguineus, Dermacentor nitens and A. cajennense were those typically found on these hosts in Brazil. The most prevalent tick species, A. cajennense, was found on both wild and domestic animals and was also prevalent in the environment. Thus, this tick species is the primary vector that allows pathogens to bridge wild and domestic animals in the Cerrado.Neste trabalho são descritas as espécies de carrapatos de animais selvagens e domésticos e do ambiente coletados por um ano na EMBRAPA Gado de Corte localizado na área urbana de Campo Grande, Mato Grosso do Sul, Brasil. Dos 55 hospedeiros selvagens de seis espécies diferentes (Nasua nasua, Cebus spp., Cerdocyon thous, Myrmecophaga tridactyla, Tamandua tetradactyla e Dasyprocta aguti foram coletados 323 carrapatos. Amblyomma ovale foi encontrado apenas em quatis e Amblyomma nodosum apenas sobre tamanduás. Nenhum carrapato foi encontrado sobre macacos-prego. Por outro lado, Amblyomma cajennense foi encontrado em todos os hospedeiros

  19. Ocorrência de Ornithonyssus bursa (Berlese, 1888 (Acari: Macronyssidae em filhotes de Megascops choliba (corujinha-do-mato e Pitangus sulphuratus (bem-te-vi, no Rio Grande do Sul, Brasil Ocurrence of Ornithonyssus bursa (Berlese, 1888 (Acari: Macronyssidae on Megascops choliba (tropical screech-owl and Pitangus sulphuratus (great kiskadee nestlings in the Rio Grande do Sul State, Brazil

    Carolina S. Mascarenhas

    2009-12-01

    Full Text Available O Núcleo de Reabilitação da Fauna Silvestre e Centro de Triagem de Animais Silvestres da Universidade Federal de Pelotas - RS atendeu dois filhotes de Megascops choliba (corujinha-do-mato (Strigiformes - Strigidae e dois de Pitangus sulphuratus (bem-te-vi (Passeriformes - Tyrannidae intensamente parasitados por ácaros, em maio de 2005 e dezembro de 2006, respectivamente. Os filhotes e o ninho de P. sulphuratus foram recolhidos na zona urbana da cidade de Pelotas - RS após forte temporal. Os ácaros foram removidos, colocados em álcool 70% e levados ao laboratório de parasitologia para identificação. Os espécimes foram clarificados em lactofenol, montados em meio de Hoyer e identificados como Ornithonyssus bursa (Acari - Macronyssidae. Registra-se Megascops choliba e Pitangus sulphuratus como hospedeiros de Ornithonyssus bursa, no Rio Grande do Sul, Brasil.The Center for Rehabilitation of Wildlife and Center for Selection of Wild Animal of the Federal University of Pelotas has attended two nestlings of Megascops choliba (tropical screech-owl (Strigiformes - Strigidae and two of Pitangus sulphuratus (great kiskadee (Passeriformes - Tyrannidae heavily parasitized by mites, in May 2005 and December 2006, respectively. The nestlings and the nest of P. sulphuratus were collected in the Pelotas urban area after severe storms. The mites were removed, clarified in lactofenol, permanently mounted in Hoyer's medium and identified as Ornithonyssus bursa (Acari - Macronyssidae. Megascops choliba and Pitangus sulphuratus are reported as host of Ornithonyssus bursa in Rio Grande do Sul State, Brazil.

  20. Mites and ticks (Acari. Chapter 7.4

    Maria Navajas

    2010-07-01

    Full Text Available The inventory of the alien Acari of Europe includes 96 species alien to Europe and 5 cryptogenic species. Among the alien species, 87 are mites and 9 tick species. Besides ticks which are obligate ectoparasites, 14 mite species belong to the parasitic/predator regime. Among these species, some invaded Europe with rodents (8 spp. and others are parasitic to birds (2 spp. The remaining 77 mite species are all phytophagous and among these 40% belong to the Eriophyidae (37 spp. and 29% to the Tetranychidae (27 spp. families. These two families include the most significant agricultural pest. The rate of introductions has exponentially increased within the 20th century, the amplification of plant trade and agricultural commodities movements being the major invasion pathways. Most of the alien mite species (52% are from North America, Asia (25%, and Central and South America (10%. Half of the ticks (4 spp. alien to Europe originated from Africa. Most of the mite species are inconspicuous and data regarding invasive species and distribution range is only partially available. More research is needed for a better understanding of the ecological and economic effects of introduced Acari.

  1. The parasitism of Ixodes luciae (Acari: Ixodidae on marsupials and rodents in Peruvian Amazon O parasitismo do Ixodes luciae (Acari: ixodidae em marsupiais e em roedores na Amazônia Peruana

    María Mónica Díaz

    2009-01-01

    Full Text Available In this work the infestation with I. luciae on Didelphimorphia and Rodentia in different environments of Peruvian Amazon was studied. Didelphimorphia was represented by the family Didelphidae. Specimens belonging to Caluromys lanatus, Didelphis marsupialis, Marmosops sp.2, Metachirus nudicaudatus, Philander andersoni and Philander opossum were infested with adults I. luciae and one Micoureus sp. was infested with larvae. In Rodentia, the infestation with I. luciae nymphs was restricted to Hylaeamys perenensis, Hylaeamys yunganus and Oligoryzomys microtis, while one Oecomys bicolor (all Cricetidae was infested with larvae of this species. The few larvae were found on rodents captured in primary forest. The only significant difference (P No presente trabalho, infestações por Ixodes luciae em Didelphimorphia e Rodentia em diferentes ambientes da Amazônia peruana foram estudadas. Didelphimorphia foi representada pela família Didelphidae. Espécimes pertencentes a Caluromys lanatus, Didelphis marsupialis, Marmosops sp., Metachirus nudicaudatus, Philander andersoni e Philander opossum foram encontrados infestadas por adultos de I. luciae; um Micoureus sp. foi encontrado infestado por larvas. Em Rodentia, a infestação por ninfas de I. luciae estiveram restritas a Hylaeamys perenensis, Hylaeamys yunganus e Oligoryzomys microtis, enquanto que um Oecomys bicolor (todos Cricetidae esteve infestado por larvas de I. luciae. As poucas larvas foram encontradas em roedores capturados na floresta primária. A única diferença significante (P < 0.05 na prevalência de carrapatos adultos em Didelphimorphia foi entre P. andersoni e M. nudicaudatus (distribuição chi-quadrado. A distribuição de carrapatos adultos foi significativamente diferente em P. andersoni quando comparado com M. nudicaudatus, P. opossum e D. marsupialis (teste Kruskal-Wallis. Nenhum efeito significante do mês ou ambiente foi observado em relação à infestação por carrapato

  2. Toxicidade diferencial de produtos à base de abamectina ao ácaro Brevipalpus phoenicis (Geijskes, 1939 (Acari: Tenuipalpidae em citros Differential toxicity of abamectin based products over Brevipalpus phoenicis (Geijskes, 1939 (Acari: Tenuipalpidae mite in citrus

    Daniel Júnior de Andrade

    2010-03-01

    Full Text Available O ácaro Brevipalpus phoenicis é uma das principais pragas dos citros por ser vetor do "Citrus Leprosis Virus" (CiLV, agente causal da leprose, uma das mais graves doenças da citricultura. Objetivou-se avaliar o efeito tóxico de produtos à base de abamectina sobre o ácaro B. phoenicis. Foram realizados um experimento de ação direta e três de ação residual no Laboratório de Acarologia do Departamento de Proteção de Plantas (Fitossanidade da FCAV - UNESP, Jaboticabal-SP. O delineamento adotado nos bioensaios foi o inteiramente casualizado, onde 10 tratamentos foram repetidos 7 vezes, sendo cada repetição composta por um fruto de laranja. Os tratamentos estudados (mL p.c./100 L de água foram: Acaramik a 20; 30; 40 e 50 mL; Vertimec a 30 e 40 mL; Abamectin Nortox a 30 e 40 mL; Tricofol a 77 mL e uma testemunha sem aplicação. Utilizaram-se frutos com presença de verrugose, que foram lavados e parcialmente parafinados, deixando-se uma área sem parafina, que foi circundada com cola entomológica para contenção dos ácaros. Transferiram-se 20 ácaros adultos B. phoenicis para cada fruto. No bioensaio de ação direta, a transferência foi realizada antes das aplicações e, nos bioensaios de ação residual, aos 5; 10 e 15 dias após a aplicação dos produtos. A aplicação dos produtos sobre os frutos foi realizada em Torre de Potter. Os resultados obtidos nos bioensaios evidenciaram que os melhores tratamentos foram: Tricofol a 77 mL, Acaramik a 40 e 50 mL e Vertimec a 40 mL. De forma geral, os produtos testados podem ser utilizados no controle do ácaro B. phoenicis.The mite Brevipalpus phoenicis (Geijskes, 1939 (Acari: Tenuipalpidae is one of the most important pests in Brazil citrus plantation, because it is the virus "Citrus Leprosis Virus" (CiLV vector, one of the most serious citrus plantation diseases. The purpose of this experiment was to evaluate the toxical effect of abamectin in the mite B. phoenicis. It was performed

  3. Development of Iphiseiodes quadripilis (Banks) (Acari: Phytoseiidae) on pollen or mite diets and predation on Aculops pelekassi (Keifer) (Acari: Eriophyidae) in the laboratory.

    Villanueva, Raul T; Childers, Carl C

    2007-02-01

    Development and reproduction of Iphiseiodes quadripilis (Banks) were evaluated on single food diets of pollen (Malephora crocea Jacquin [ice plant] or Quercus sp. [oak]), spider mites, [Eutetranychus banksi (McGregor) or Panonychus citri (McGregor) (Acari: Tetranychidae)], or the citrus rust mite Phyllocoptruta oleivora (Ashmead) (Acari: Eriophyidae). Experiments were conducted in an environmental chamber at 28 degrees +/- 1 degrees C, 14:10 (L:D) daylength, and 45% RH. I. quadripilis completed development and laid viable eggs that subsequently hatched on diets of either ice plant or oak pollen or eggs and motile stages of E. banksi. P. citri was acceptable as prey, but survival of larvae to adults was only 36%, whereas survival on E. banksi, ice plant pollen, and oak pollen was 48, 60, and 68%, respectively. The webbing produced by P. citri seemed to inhibit foraging behavior of I. quadripilis larvae and nymphs. Larvae of I. quadripilis developed only to the second nymphal instar on a diet of P. oleivora alone or water alone. Starved I. quadripilis females and deutonymphs were observed preying on the pink citrus rust mite, Aculops pelekassi (Keifer) (Eriophyidae). During 4-min observation trials, two series of I. quadripilis fed on 1.8 +/- 0.47 and 3.5 +/- 0.45 A. pelekassi motile stages after being starved for 6 and 24 h, respectively. I. quadripilis females did not prey on P. oleivora in arenas containing both rust mite species. PMID:17349110

  4. Fatores que afetam artrópodes associados a cinco acessos de ginseng-brasileiro (Pfaffia glomerata) em Montes Claros, Estado de Minas Gerais = Factors affecting arthropods associated with five accessions of Brazilian ginseng (Pfaffia glomerata) in Montes Claros, Brazil

    Germano Leão Demolin Leite; Mayra Pimenta; Péricles Leonardo Fernandes; Ronnie Von Santos Veloso; Ernane Ronnie Martins

    2008-01-01

    O objetivo deste trabalho foi identificar artrópodes associados a cinco acessos ('NDS', 'COVB', 'NAT', 'ROST' e 'GSD1') de Pfaffia glomerata, bem como o efeito de clima, dossel, face foliar, tricomas e inimigos naturais sobre a entomofauna. Dos artrópodes observados, Aphis gossypii (Hemiptera: Aphididae), Diabrotica speciosa (Coleoptera: Chrysomelidae) e Tetranychus ludeni (Acari: Tetranychidae) apresentaram maior densidade populacional. Dos inimigos naturais observados, ácaros predadores est...

  5. Pyrethroid resistance in Phytoseiulus macropilis (Acari: Phytoseiidae): cross-resistance, stability and effect of synergists.

    Queiroz, Maria Cristina Vitelli; Sato, Mario Eidi

    2016-01-01

    Phytoseiulus macropilis Banks (Acari: Phytoseiidae) is an effective predator of Tetranychus urticae Koch (Acari: Tetranychidae). The objectives of this research were to study the stability of fenpropathrin resistance and the cross-resistance relationships with different pyrethroids, and also to evaluate the effect of synergists [piperonyl butoxide (PBO), diethyl maleate (DEM) and S,S,S-tributyl phosphorotrithioate (DEF)] on fenpropathrin resistant and susceptible strains of this predaceous mite. The stability of fenpropathrin resistance was studied under laboratory conditions, using P. macropilis populations with initial frequencies of 75 and 50% of resistant mites. The percentages of fenpropathrin resistant mites were evaluated monthly for a period of up to 12 months. A trend toward decreased resistance frequencies was observed only during the first 3-4 months. After this initial decrease, the fenpropathrin resistance was shown to be stable, maintaining constant resistance frequencies (around 30%) until the end of the evaluation period. Toxicity tests carried out using fenpropathrin resistant and susceptible strains of P. macropilis indicated strong positive cross-resistance between fenpropathrin and the pyrethroids bifenthrin and deltamethrin. Bioassays with the synergists DEM, DEF and PBO were also performed. The maximum synergism ratio (SR = LC50 without synergist/LC50 with synergist) detected for the three evaluated synergists (PBO, DEM, DEF) was 5.86 (for DEF), indicating low influence of enzyme detoxification processes in fenpropathrin resistance. PMID:26530989

  6. INTERACCIÓN ENTRE DOS ÁCAROS DEPREDADORES DE Tetranychus urticae KOCH (ACARIFORMES: TETRANYCHIDAE EN LABORATORIO Interaction Between Two Predator Mites of Tetranychus urticae Koch (Acariformes: Tetranychidae in Laboratory

    ANGÉLICA ARGÜELLES R

    2013-04-01

    Full Text Available Tetranychus urticae (Acari: Tetranychidae es una de las principales plagas de cultivos ornamentales, entre las especies más utilizadas para su control se encuentran Neoseiulus californicus y Phytoseiulus persimilis (Parasitiformes: Phytoseiidae. En el presente trabajo se propone el manejo de la plaga mediante el empleo de liberaciones simultáneas de los dos fitoseidos. Se evaluaron varias situaciones, por un lado se estudiaron las interacciones cuando un depredador se encuentra en una densidad baja mientras que el otro depredador se presenta en alta densidad (esta situación se analizó tanto en presencia como en ausencia de la presa. Por otro lado, se evaluaron las interacciones cuando los P. persimilis y N. californicus dos están presentes en igual densidad y en presencia de T. urticae. Cuando uno de los depredadores está en mayor densidad y hay presencia de la presa, se observa que al incrementar la edad del depredador que tiene menor densidad, aumenta también la interferencia en el consumo de presas por parte de los depredadores que están en mayor densidad. Además cuando disminuye el consumo de T. urticae se incrementa el consumo intraguilda. Phytoseiulus persimilis en ausencia de T. urticae y en presencia de N. californicus adopta un comportamiento de depredación intraguilda sobre todos los estados de desarrollo de su conespecifico, mientras que N. californicus únicamente consume larvas de conespecíficos en ausencia del fitófago y en presencia de P. persimilis. Cuando se encontraban los dos depredadores en el mismo montaje y la misma densidad de población, no se observó un mayor consumo de T. urticae que cuando cada depredador es empleado por separado.Tetranychus urticae (Acari: Tetranychidae is an important pest of ornamental crops. A species of predatory mite used for its control is Neoseiulus californicus and Phytoseiulus persimilis (Acari: Phytoseiidae. This research proposes the use of joint releases of the two cited

  7. Efeito do controle estratégico de Amblyomma cajennense (Fabricius, 1787 (acari: ixodidae sobre a população de Anocentor nitens (Neumann, 1897 (acari: ixodidae em eqüinos Effect of the strategic control of Amblyomma cajennense (Fabricius, 1787 (acari: ixodidae on the population of Anocentor nitens (Neumann, 1897 (acari: ixodidae in equines

    Arildo P. Da Cunha

    2007-12-01

    Full Text Available Objetivou-se avaliar o efeito de um programa de controle estratégico de Amblyomma cajennense sobre a população de Anocentor nitens em eqüinos. Os tratamentos carrapaticidas foram realizados a cada sete dias e divididos em dois módulos, o primeiro com início em abril de 2004 (oito tratamentos e o segundo com início em julho do mesmo ano (cinco tratamentos, utilizando-se a base química piretróide - cipermetrina na concentração de 0,015%. Avaliou-se mensalmente a carga parasitária de A. nitens no pavilhão auricular e divertículo nasal dos eqüinos antes (outubro de 2003 a março de 2004 e depois (outubro de 2004 a março de 2005 do controle estratégico com banhos carrapaticidas. Utilizaram-se escores de 0 a 3 para classificar os níveis de infestação. Entretanto, não houve redução significativa da carga parasitária de A. nitens no pavilhão auricular e no divertículo nasal dos eqüinos, possivelmente, devido a não aplicação sistemática de carrapaticida no divertículo nasal, pois, esta estrutura anatômica é um importante sítio de infestação por A. nitens em eqüinos.The objective of this study was to evaluate the effect of the Amblyomma cajennense strategic control program on the Anocentor nitens population. The acaricide treatments were carried out every seven days and divided in two series, the first one beginning in April 2004 (eight treatments and the second beginning in July of the same year (five treatments, using pyrethroid chemical base - cypermethrin in the concentration of 0,015%. Monthly it was evaluated the infestation of A. nitens in the ear and nasal diverticulum of the equines before (October 2003 to March 2004 and after (October of 2004 to March of 2005 the strategic program of acaricide treatments. It was used score of 0 to 3 to classify the infestation levels. It did not have any significant reduction of the parasitic load of A. nitens in the ear and nasal diverticulum of the equines. Possibly, due

  8. Parasitismo em humano por Amblyomma sp (Acari: Ixodidae, na cidade de Recife, estado de Pernambuco Parasitism by Amblyomma sp (Acari: Ixodidae in humans in the city of Recife, state of Pernambuco, Brazil

    Rafael Antonio do Nascimento Ramos

    2010-10-01

    Full Text Available INTRODUÇÃO: Os ixodídeos pertencentes ao gênero Amblyomma são encontrados no Brasil, sendo de grande importância em Saúde Pública por representarem risco na transmissão de patógenos. MÉTODOS: Este trabalho relata um caso de parasitismo em humano por Amblyomma sp, ocorrido na Cidade de Recife, Pernambuco. RESULTADOS: O parasitismo foi observado em uma senhora onde os ectoparasitos encontravam-se fixados em suas pernas e pés. CONCLUSÕES: Este tipo de achado é incomum e constitui o primeiro relato do parasitismo em humano por Amblyomma sp, no Estado de Pernambuco.INTRODUCTION: The Amblyomma genus is widely distribution in Brazil and is important regarding the public health risk represented by the transmission of pathogens. METHODS: This paper reports a case of parasitism in humans by Amblyomma sp that occurred in the City of Recife, State of Pernambuco. RESULTS: Parasitism was observed in a woman where the ticks were attached to her legs and feet. CONCLUSIONS: This finding is unusual and is the first report of parasitism in humans by Amblyomma sp in State of Pernambuco.

  9. Survey of natural enemies of spider mites (Acari: Tetranychidae) in citrus orchards in eastern Spain.

    Abad-Moyano, Raquel; Pina, Tatiana; Dembilio, Oscar; Ferragut, Francisco; Urbaneja, Alberto

    2009-01-01

    Field surveys were conducted from 2004 to 2007 to determine the species composition and relative abundance of natural enemies associated with colonies of either the citrus red mite, Panonychus citri, or the two spotted spider mite, Tetranychus urticae, in Valencian citrus orchards (eastern Spain). Fourteen species were recorded, six phytoseiid mites and eight insect predators. Two of them are reported for the first time on citrus in Spain and two more are first reports as predators associated with T. urticae. The community of predators associated with T. urticae and P. citri was almost identical, and the Morisita-Horn index of similarity between both natural enemy complexes was close to one, suggesting that predators forage on both pest species. Quantifying the presence of many known spider mites predators in Valencian citrus orchards is an important first step towards spider mite control. A challenge for future studies will be to establish conservation and/or augmentation management strategies for these predators, especially to improve T. urticae biological control. PMID:18803026

  10. Molecular Effects of Irradiation (Cobalt-60) on the Control of Panonychus citri (Acari: Tetranychidae).

    Zhang, Ke; Luo, Lingyan; Chen, Xieting; Hu, Meiying; Hu, Qiongbo; Gong, Liang; Weng, Qunfang

    2015-01-01

    The effective dose of irradiation to control pest mites in quarantine has been studied extensively, but the molecular mechanisms underlying the effects of the irradiation on mites are largely unknown. In this study, exposure to 400 Gy of γ rays had significant (p Panonychus citri. The irradiation caused the degradation of the DNA of P. citri adults and damaged the plasma membrane system of the egg, which led to condensed nucleoli and gathered yolk. Additionally, the transcriptomes and gene expression profiles between irradiated and non-irradiated mites were compared, and three digital gene expression libraries were assembled and analyzed. The differentially expressed genes were putatively involved in apoptosis, cell death and the cell cycle. Finally, the expression profiles of some related genes were studied using quantitative real-time PCR. Our study provides valuable information on the changes in the transcriptome of irradiated P. citri, which will facilitate a better understanding of the molecular mechanisms that cause the sterility induced by irradiation. PMID:26569230

  11. Population dynamics of Panonychus osmanthi (Acari: Tetranychidae) on two Osmanthus species.

    Kitashima, Yasuki; Gotoh, Tetsuo

    2003-01-01

    Panonychus osmanthi is a non-diapausing species of spider mite that superficially resembles P. citri. It infests Osmanthus species, which are evergreen roadside and garden trees. The population dynamics of P. osmanthi were studied on Osmanthus aurantiacus and O. x fortunei during a three-year period. Seasonal changes in P. osmanthi populations were fundamentally the same in each year, although their density differed greatly from year to year. The P. osmanthi population was bimodal, with one peak in spring (May-June) and another in winter (November-January). Populations abruptly declined after the spring peak. Predators showed a delayed density-dependent response to changes in spider mites from spring to summer, whereas in autumn and winter, predators were few because they had entered diapause. To determine the effect of predators on the rapid decline of spider mites just after the spring peak, the predators were removed by treating the trees with a synthetic pyrethroid. As a result, spider mite density did not decline after the spring peak and remained at a high level during the June-August period when spider mite density is usually very low. This suggests that predators play an important role in the drastic decline of P. osmanthi density just after the spring peak. PMID:14635810

  12. Significance of habitat type for the genetic population structure of Panonychus citri (Acari: Tetranychidae).

    Osakabe, Masahiro; Goka, Kouichi; Toda, Satoshi; Shintaku, Toshiyuki; Amano, Hiroshi

    2005-01-01

    Restricted migration and habitat fragmentation promote genetic differentiation between populations. Because most of the hosts of Panonychus citri are woody plants, mainly citrus trees that are usually planted at intervals of several metres, this mite likely faces more risks (e.g., starvation) by dispersing between host plants, compared to other spider mite species that infest both herbaceous and woody plants, such as Tetranychus urticae. Such a limited gene flow between patches (host plants) can lead to differentiation of populations even within a small area. Therefore, we hypothesize that P. citri populations are genetically differentiated not only between distant populations but also within small areas, such as within a grove. To test this hypothesis, we investigated the divergence of P. citri populations in Japanese citrus groves according to a hierarchical arrangement of geographical distance, ranging from distant populations (10 groves distributed throughout different areas in two major Japanese islands; this level of analysis is referred to as 'geographic') to local populations (different trees in a specific grove; 'local'). Three molecular markers were used: an esterase locus, one microsatellite and a point mutation in the mitochondrial cytochrome oxidase subunit I. At a local level acaricide susceptibility tests were also performed using two acaricides: fenpyroximate (25 ppm) and etoxazole (3.33 ppm). At a broad geographic level the gene diversity decreased with decreasing area size and distance between populations. By contrast, at the local level, populations maintained a significant level of variation between trees within groves, and the divergence within groves was higher than between groves. Whereas no statistical difference of the mortalities was detected among groves for the two acaricides tested, the difference was statistically significant among trees within groves in fenpyroximate (ANOVA, pcitri populations maintain a higher level of variation between trees (or patches of trees) within groves than between groves at the local level, though the gene diversity tended to be smaller with decreasing distance between populations at the geographical level. Results are discussed in relation to the dispersal behaviour of spider mites. PMID:16082921

  13. Interspecific diversity of mitochondrial COI sequences in Japanese Panonychus species (Acari: Tetranychidae).

    Toda, S; Osakabe, M; Komazaki, S

    2000-01-01

    Sequences of a part of the mitochondrial cytochrome oxidase subunit I (COI) gene were analyzed in four Japanese Panonychus species to determine their phylogenetic relationships. Neighbor-Joining and maximum likelihood analysis resulted in a high bootstrap support of the relationships within the genus Panonychus. In contrast with a previous study based on ribosomal DNA data, the COI phylogeny suggested that P. mori was more distantly related to P. citri than to P. ulmi. This study shows for the first time that P. osmanthi is closely related to P. citri. Intraspecific variation analysis shows that the genetic distance between two local populations of P. mori is higher than between P. citri and P. osmanthi. PMID:11345318

  14. RFLP analysis of ribosomal DNA in sibling species of spider mite, genus Panonychus (Acari: Tetranychidae).

    Osakabe, M; Sakagami, Y

    1994-02-01

    Ribosomal DNA (rDNA) restriction fragment length of spider mites was analysed in three sibling species of genus Panonychus (P. mori, P. citri and P. ulmi) and Tetranychus urticae. Seventy-one fragments were detected by enhanced chemiluminescence (ECL) after Southern hybridization with two genomic rDNA probes of mouse. Percentages of shared fragments within species were larger than those among species, indicating that the variation of rDNAs was useful in phylogenetic studies of spider mite species. In a phenogram based on differences of the rDNA fragment length, the relationship among Panonychus species was compatible with the results of analysis based on the morphological characters. PMID:7915173

  15. Molecular characterization of two carboxylesterase genes of the citrus red mite, Panonychus citri (Acari: Tetranychidae).

    Zhang, Kun; Niu, Jin-Zhi; Ding, Tian-Bo; Dou, Wei; Wang, Jin-Jun

    2013-04-01

    The citrus red mite, Panonychus citri, is known for its ability to rapidly evolve resistance to insecticides/acaricides and to adapt to hosts that produce toxins. To get better insight into the detoxification mechanism of P. citri, two carboxylesterase (CarE) genes, PCE1 and PCE2, were isolated and characterized. PCE1 and PCE2 contained open reading frames of 1,653 and 1,392 nucleotides, encoding proteins of 550 and 463 amino acid residues, respectively. Phylogenetic analyses showed that PCE1 and PCE2 were most closely related to the CarE genes from other phytophagous mites. The transcriptional profiles of two CarE genes among developmental stages (egg, larva, nymph, adult female, and adult male), after exposing to four acaricides (avermectin, azocyclotin, pyridaben, and spirodiclofen) and acid rain were investigated using real-time quantitative PCR (qPCR). The results showed that during development, PCE1 was highly expressed at the egg stage, whereas PCE2 was abundantly expressed at the adult stage of males. The expression levels of PCE1 were highly induced upon exposure to acaricides and acid rain. On the other hand, the expression levels of PCE2 were increased after treatment with avermectin and pyridaben. These results suggest that PCE1 and PCE2 may have distinct roles in different developmental stages and participate in the detoxification of acaricides. PMID:23404785

  16. Molecular Effects of Irradiation (Cobalt-60) on the Control of Panonychus citri (Acari: Tetranychidae)

    Ke Zhang; Lingyan Luo; Xieting Chen; Meiying Hu; Qiongbo Hu; Liang Gong; Qunfang Weng

    2015-01-01

    The effective dose of irradiation to control pest mites in quarantine has been studied extensively, but the molecular mechanisms underlying the effects of the irradiation on mites are largely unknown. In this study, exposure to 400 Gy of γ rays had significant (p < 0.05) effects on the adult survival, fecundity and egg viability of Panonychus citri. The irradiation caused the degradation of the DNA of P. citri adults and damaged the plasma membrane system of the egg, which led to condensed...

  17. Life-history traits of the six Panonychus species from Japan (Acari: Tetranychidae).

    Gotoh, Tetsuo; Ishikawa, Yukio; Kitashima, Yasuki

    2003-01-01

    Six species of the genus Panonychus are known from Japan. Life-history parameters of all six species were investigated at 25 degrees C, and for three species two strains of different geographical origin were included. The intrinsic rate of natural increase (r(m)) ranged from 0.172/day for the P. osmanthi albino strain to 0.209/day for P. citri, while the net reproductive rate (R0) varied from 23.98 in the thelytokous species P. thelytokus to 46.61 in P. citri. Both values were higher in the polyphagous species (P. ulmi, P mori and P. citri), which are considered crop pests, than those in the oligophagous species (P. thelytokus and P. osmanthi), considered non-pests. The only exception was P. bambusicola, an oligophagous non-pest species with R0- and r(m)-values closely resembling those of the three polyphagous species. PMID:14635811

  18. The Effect of Spiromesifen on the Reproductive Potential of Tetranychus urticae Koch (Acari: Tetranychidae

    Dejan Marčić

    2009-01-01

    Full Text Available Laboratory bioassays were conducted to evaluate the effects of spiromesifen on the fecundity, fertility and population growth of two-spotted spider mite (Tetranychus urticae Koch after treatment of pre-ovipositing females with five acaricide concentrations: 180mg/l (maximum recommended concentration for use in glasshouses against spider mites, 18 mg/l, 1.8 mg/l and 0.18 mg/l (the last one was discriminative for eggs and immatures in preliminary studies, i.e. produced 100% mortality of those stages and 0.018 mg/l. After24h exposure, the percentages of females surviving treatment without visible symptoms of poisoning were 50% (180 mg/l, 45% (18 mg/l, 51% (1.8 mg/l, 74% (0.18 mg/l, 96% (0.018 mg/l and 98% (0 mg/l. Over the first four days after treatment, the females that survived180 mg/l and 18 mg/l laid no eggs. The total number of eggs laid after treatment with these two concentrations was reduced to less than 2% against the control by the end of the trial. The females that survived 1.8 mg/l laid 50% less eggs, compared to the control, while the number of eggs laid by the females treated with 0.18 mg/l and 0.018 mg/l were 19% and 4% lower, respectively. Over the initial four days, egg hatch rates in treatments were 73-87%, and 92-93% in the control. Significant statistical differences between gross fecundity (FCg and gross fertility (FTg values in the control and treatments were detected for females surviving 180 mg/l, 18 mg/l and 1.8 mg/l. On the other hand, only the net fertility (FTn value of females treated with 0.018 mg/l showed no statistically significant difference from the control value. Treatments with 180 mg/l and 18 mg/l significantly reduced the instantaneous rate of increase (ri 6, 8 and 10 days after treatment, compared to the control. The negative ri values in those treatments indicated a declining population. Sublethal effects of spiromesifen and its impact on T. urticae management are discussed.

  19. Reproductive parameters of Tetranychus urticae (Acari: Tetranychidae) affected by neonicotinoid insecticides.

    Barati, Reihaneh; Hejazi, Mir Jalil

    2015-08-01

    Two-spotted spider mite is a major pest of many agricultural and ornamental crops worldwide. Some reports have indicated that application of neonicotinoid insecticides may lead to increased fecundity of this pest. If this is found to be true, the use of these pesticides may cause an outbreak of spider mite populations. Sublethal effects of three neonicotinoids, namely thiacloprid, acetamiprid and thiamethoxam were studied on T. urticae adults at field recommended doses. The experiments were carried out using bean leaf pieces in plastic Petri dishes. The adult mites were treated using two methods: (1) drench application and (2) spraying of leaves with Potter Spray Tower. Our results indicated that all neonicotinoids tested increased T. urticae population. In both treatment methods, acetamiprid treated mites had the highest intrinsic rate of population increase (rm) and finite rate of population increase (λ); and the lowest mean generation time (T) and doubling time among the treatments. If similar results are obtained from greenhouse and field trials, the use of these insecticides requires necessary precautions such as avoiding repeated use of neonicotinoid insecticide for controlling insect pests. PMID:25912952

  20. Functional response of Phytoseiulus macropilis under different Tetranychus urticae (Acari: Phytoseiidae, Tetranychidae population density in laboratory

    Noeli Juarez Ferla

    2011-02-01

    Full Text Available We tested the functional response of the phytoseiid predator Phytoseiulus macropilis (Banks, 1904 to different population densities of Tetranychus urticae Koch, 1836 (the two-spotted spider mite - TSSM in the laboratory. We evaluated the curve that best fits the data obtained, based on the reduced X². The predators were obtained from leaves of Oso Grande strawberries at the municipality of Bom Princípio, state of Rio Grande do Sul, Brazil. Fifteen seemingly healthy females of P. macropilis were randomly chosen and individualized in each of the arenas with different TSSM densities. The following prey densities were tested: 1, 2, 3, 5, 10, 20, 30, 35, 45, 55, 70 and 125 females. The tests were performed in a germination chamber with photoperiod of 12 hours at 28 ± 1ºC per day and 22 ± 1ºC at night and 80 ± 5% relative humidity. In all tests with up to three prey items, P. macropilis showed low functional response, whereas higher rates were observed when more prey items were offered. There was a strong positive correlation between increased prey numbers and daily prey consumption (r = 0.84 and p = 0.0006. A significant correlation was found between prey consumption and total oviposition (r = 0.70, p = 0.01. The daily oviposition rate was lower when few prey items were offered, increasing significantly with increased prey availability. The results showed a positive correlation between the number of prey items offered to the predator and its oviposition rate (r = 0.66, p = 0.01. Nevertheless, a strong correlation was observed between the daily consumption of prey and eggs/female/day (r = 0.92, p < 0.0001. A shorter oviposition period was reported when up to three prey items were offered. The oviposition period and the increase in prey numbers were positively correlated (r = 0.30, p = 0.34, but the difference was not statistically significant. Nevertheless, the longer the egg-laying period, the greater the oviposition rate (r = 0.90, p < 0.0001. The curve that best fits the data obtained in each case was also calculated, based on the least square method.

  1. Stability and fitness costs associated with etoxazole resistance in Tetranychus urticae (Acari: Tetranychidae).

    Stocco, Rafael Sorrentino Minazzi; Sato, Mário Eidi; Santos, Taiana Lumi

    2016-08-01

    The two-spotted spider mite, Tetranychus urticae Koch, is one of the most important pests on a wide range of crops worldwide. Studies on stability of resistance and possible fitness costs associated with etoxazole resistance were carried out in T. urticae to provide basic information necessary to define effective acaricide resistance management strategies for this pest. Selection for resistance to etoxazole was performed in a population of T. urticae collected from a commercial rose field, in Holambra County, in the State of São Paulo, Brazil. After five rounds of selection for resistance, the resistance ratio (R/S) at the LC50 reached 8739 fold value in comparison with a susceptible strain (Brazabrantes S Strain). The stability of etoxazole resistance was studied under laboratory conditions, using a population with initial frequency of 75 % of resistant mites. The frequencies of etoxazole resistance were evaluated monthly for a period of 13 months. The frequency of etoxazole resistance decreased from 75 to 37 % in this period. Comparison of biological traits between resistant and susceptible strains indicated the presence of fitness costs associated with etoxazole resistance. The resistant strain showed lower fecundity and a higher proportion of males in the population. The figures for net reproductive rate (R 0), intrinsic rate of natural increase (r m) and finite rate of increase (λ) were higher in the susceptible strain. The instability of etoxazole resistance is a favorable aspect for the acaricide resistance management in the spider mite. PMID:27222145

  2. Greenhouse and Field Evaluation of Two Biopesticides Against Tetranychus urticae and Panonychus ulmi (Acari: Tetranychidae

    Dejan Marčić

    2012-01-01

    Full Text Available The mycopesticide Naturalis (based on Beauveria bassiana strain ATCC 74040 andbotanical pesticide Kingbo (based on oxymatrine, an alkaloid from Sophora flavescens, a traditionalChinese herb were tested against the two-spotted spider mite (Tetranychus urticaeon greenhouse vegetables and the European red mite (Panonychus ulmi on apples. Thesebiopesticide products were applied twice at 5-day interval and concentrations of 0.1% and0.2%, and their effectiveness was compared to abamectin-based products and the syntheticacaricides acrinathrin and spirodiclofen, applied once at their recommended rates.The mycopesticide Naturalis, applied at 0.1% concentration against T. urticae on cucumber,reduced mite population density by 85-86%, achieving 91-93% efficacy. In a trial on tomato,efficacy reached some 96%, while population density was reduced by 93%. In a field trialon apple, Naturalis demonstrated an increasing and long-lasting effectiveness against thesummer population of P. ulmi of nearly 100%, and population reduction was achieved inassessments 30 days after the first treatment. Naturalis applied at a double rate achieved asomewhat better effect but only in the first trial. The botanical pesticide Kingbo, applied at0.1% concentration, demonstrated very high control efficacy (≥98% and population densityreduction (≥96% of T. urticae in both trials. A high and long-lasting effectiveness of thisbioacaricide was also achieved in a trial on P. ulmi. Its concentration of 0.2% achieved similareffect. The results in these trials indicate that applications of the mycopesticide Naturalisand the botanical pesticide Kingbo can provide effective control of T. urticae on cucumberand tomato grown in greenhouses, as well as P. ulmi on apple.

  3. Tetranychus urticae (Acari: Tetranychidae) transmits Acidovorax citrulli, causal agent of bacterial fruit blotch of watermelon.

    Choi, Okhee; Park, Jung-Joon; Kim, Jinwoo

    2016-08-01

    The two-spotted spider mite (TSSM) Tetranychus urticae is one of the most important pests of cucurbit plants. If TSSM can act as vector for Acidovorax citrulli (Acc), causal agent of bacterial fruit blotch (BFB), then the movement of mites from infected to healthy plants may represent a potential source of inocula for BFB outbreaks. To confirm the association between Acc and TSSM, we generated a green fluorescent protein-tagged mutant strain (Acc02rf) by transposon mutagenesis and demonstrated that TSSM can transmit Acc from infected to non-infected watermelon plants. Challenge with 10 TSSMs carrying Acc02rf population densities of 1.3 × 10(3) CFU each on freshly grown individual watermelon plants caused disease transmission to 53 %. Incubation periods ranged 7-9 days. Bacteria recovered from symptoms typical of those associated with leaf necrosis were characterized and identified as Acc. To our knowledge, this is the first report showing that TSSM can be a vector of Acc. The results reported here support that the strong association of TSSM with Acc is of particular importance in controlling BFB. PMID:27178042

  4. Toxicity of neem oil to the cassava green mite Mononychellus tanajoa (Bondar) (Acari: Tetranychidae)

    Amanda C.B Silva; Adenir V. Teodoro; Oliveira, Eugênio E.; Adriano S Rêgo; Silva, Rafael R.

    2013-01-01

    Neem (Azadirachta indica A. Juss.)-derived pesticides have been used against a wide range of agricultural pests including tetranychid mites. Approaches combining lethal and sublethal toxicity studies of neem pesticides towards tetranychid mites are necessary to a comprehensive evaluation of such products. Here, we evaluated the lethal and sublethal toxicity of the neem oil Bioneem to the cassava green mite Mononychellus tanajoa (Bondar) by integrating lethal concentration (LC) with population...

  5. Comparative life table analysis of Tetranychus urticae (Acari: Tetranychidae) on 14 soybean genotypes

    Amin Sedaratian; Yaghoub Fathipour; Saeid Moharramipour

    2011-01-01

    The life table parameters of two-spotted spider mite (TSSM), Tetranychus urticae Koch, on 14 soybean genotypes (Sari, Sahar, Tellar, Zane, Ks3494, L17, Dpx, 032,033, Clark, Hill, I27, Tms and Williams) were evaluated at 28 ± 1℃, 65% ± 5% RH and a photoperiod of 16 ∶ 8 h L ∶ D. The survivorship data (lx) of adult females were fitted to the Weibull frequency distribution model in all genotypes tested. Based on the Weibull parameters, the survival curve was type Ⅰ on all genotypes, which indicated that the mortality mostly occurred in old individuals. Furthermore, two mathematical models (Analytis and Enkegaard) were fitted to age-specific fecundity data (mx). The highest intrinsic rate of increase (rm) was recorded on L17 (0.392) and the lowest values of this parameter were obtained on Tms (0.233), Hill (0.236), 032 (0.236), I27 (0.237) and Clark (0.240). In addition, net reproductive rate (R0) and finite rate of increase (λ) of the TSSM had the highest value on L17 as 45.521 and 1.475, respectively. The lowest values of these parameters were recorded on Tms as 12.149 and 1.258, respectively. Doubling time (DT)varied significantly on different genotypes and the shortest and longest values of this period were obtained on L17 and 032, respectively. Our findings revealed that Tms, Hill,032, I27 and Clark were less suitable genotypes, suggesting that they are more resistant to the TSSM than the other genotypes.

  6. Panonychus from Georgia: survey, taxonomical status and redescription of P. hadzhibejliae (Reck, 1947) (Acari, Tetranychidae).

    Arabuli, Tea; Çobanoglu, Sultan; Auger, Philippe

    2016-01-01

    A survey of Panonychus species was undertaken across Georgia from 2005 to 2014 on various host plants, revealing three species: Panonychus citri (McGregor, 1916), Panonychus hadzhibejliae (Reck, 1947) and Panonychus ulmi (Koch, 1836). New hosts for P. ulmi and P. citri are recorded, Buxus sempervirens, Hedera colchica and Prunus laurocerasus for P. ulmi and Ficus carica for P. citri, whereas P. hadzhibejliae was only found on F. carica. The newly collected material also allowed us to investigate the taxonomical status of P. hadzhibejliae. The comparison of P. hadzhibejliae with the two closely related species sampled in the survey, P. ulmi and P. citri, and with data of P. caricae found in the literature, shows that P. hadzhibejliae is a valid species. It can be separated from the three other Panonychus species without ambiguity especially using the female dorsal setae length in combination with the ratio between the length of the female dorsal opisthosomal f2 and h1 setae and the ratio between the palptarsal terminal eupathidium su and the related solenidion ω. A redescription of P. hadzhibejliae is provided including the male and some morphological characters, measurements and drawings of the female that were omitted in the original description. A key to the world species of Panonychus is also proposed. PMID:27395141

  7. Fundamental aspects of genetic control of the two-spotted spider mite Tetranychus urticae (Acari: Tetranychidae)

    Radiobiological properties of Tetranychus urticae and other topics of genetic control have been evaluated. The induction by X-rays or fast neutrons of dominant lethals in mature sperm and of dominant lethals and recessive lethals in prophase-1 oocytes and the induction by both radiation types of chromosome mutations, recessive lethals and factors causing F1-infertility in sperm and oocytes, have been studied. From the results the optimal dose, radiation type and germ cell type could be chosen for obtaining either fully sterile males or substerile males, producing fully infertile F1-females. Also the most favourable conditions were determined for the induction of chromosome mutations with the lowest frequency of linked recessive lethals. The radiobiological properties of holokinetic chromosomes are extensively discussed. The successful displacement of the standard karyotype by a radiation arranged karyotype is presented and discussed in its relevance for practical application. (Auth./C.F.)

  8. A Simulation Model of the Mass Rearing of Tetranychus urticae Koch (Acari: Tetranychidae) on Beans.

    Bustos, A; Rodríguez, D; Cure, J R; Cantor, F

    2016-06-01

    The supply of predatory mites as natural enemies is a key component to guarantee the success of biological pest control programs as alternatives to chemical control in commercial crops. To meet the demand for a supply of biologicals, the mass rearing of natural enemies is an option, and the first step must be to develop a standardized system that maximizes the production of prey. One choice for this first step is to use simulation models that can evaluate scenarios that are difficult or complex to address experimentally. In this work, a model was developed to evaluate the current management conditions for the mass rearing of the pest mite Tetranychus urticae Koch. Our aim was to identify alternative scenarios to maximize mite production through mass rearing that could be evaluated in real systems. We assumed that populations of T. urticae were regulated by the conditions of supply-demand theory and modeled the age structure, temperature effects, and individual phenology of T. urticae. The supply-demand theory of resources was used to regulate populations, which involved structured ages and temperature effects for the different stages in the development of individuals. We used the functional response and the paradigm of metabolic pool models to describe resource acquisition and allocation. We demonstrated that 7- to 14-day-old plants infested with 45 or 62 T. urticae/plant could reach 25,000 individuals/plant, being 50% of these preys at the preferred stages by the predator Phytoseiulus persimilis Athias-Henriot. Our theoretical model requires validation in experimental/real systems of mass rearing to better verify the validity of all of the parameters and predictions before commercial implementation. PMID:26943232

  9. Toxicity of neem oil to the cassava green mite Mononychellus tanajoa (Bondar (Acari: Tetranychidae

    Amanda C.B Silva

    2013-09-01

    Full Text Available Neem (Azadirachta indica A. Juss.-derived pesticides have been used against a wide range of agricultural pests including tetranychid mites. Approaches combining lethal and sublethal toxicity studies of neem pesticides towards tetranychid mites are necessary to a comprehensive evaluation of such products. Here, we evaluated the lethal and sublethal toxicity of the neem oil Bioneem to the cassava green mite Mononychellus tanajoa (Bondar by integrating lethal concentration (LC with population growth and biological parameter studies. According to Probit analyses the concentration of neem oil Bioneem which kills 50% of the population (LC50 of M. tanajoa was 3.28 ¼L cm-2, which is roughly twice the field concentration recommended of this biopesticide to control pest mites (1.7 ¼L cm-2. The growth rate of the cassava green mite steadily decreased with dosages of neem oil. Furthermore, sublethal concentrations of the neem oil corresponding to the LC50 reduced the periods of the immature stages of M. tanajoa resulting in a shorter developmental time. Similarly, the number of eggs per day and the number of eggs per female per day, a proxy for fecundity, were drastically reduced in M. tanajoa females exposed to the LC50 of neem oil. Based on our comprehensive approach we conclude that the neem oil showed lethal as well as sublethal toxicity on growth rate and biological parameters such as duration of immature stages and fecundity of the cassava green mite M. tanajoa and it could be used as an ecological alternative for the management of this pest.

  10. Molecular Effects of Irradiation (Cobalt-60 on the Control of Panonychus citri (Acari: Tetranychidae

    Ke Zhang

    2015-11-01

    Full Text Available The effective dose of irradiation to control pest mites in quarantine has been studied extensively, but the molecular mechanisms underlying the effects of the irradiation on mites are largely unknown. In this study, exposure to 400 Gy of γ rays had significant (p < 0.05 effects on the adult survival, fecundity and egg viability of Panonychus citri. The irradiation caused the degradation of the DNA of P. citri adults and damaged the plasma membrane system of the egg, which led to condensed nucleoli and gathered yolk. Additionally, the transcriptomes and gene expression profiles between irradiated and non-irradiated mites were compared, and three digital gene expression libraries were assembled and analyzed. The differentially expressed genes were putatively involved in apoptosis, cell death and the cell cycle. Finally, the expression profiles of some related genes were studied using quantitative real-time PCR. Our study provides valuable information on the changes in the transcriptome of irradiated P. citri, which will facilitate a better understanding of the molecular mechanisms that cause the sterility induced by irradiation.

  11. Controle estratégico de Amblyomma cajennense (Fabricius, 1787 (acari: xodidae em eqüinos, Minas Gerais, Brasil - Parte I Strategic control of Amblyomma cajennense (Fabricius, 1787 (acari: ixodidae on horses, Minas Gerais, Brazil - Part I

    Arildo P. Da Cunha

    2007-12-01

    Full Text Available Objetivou-se verificar a eficiência de um programa de controle estratégico de Amblyomma cajennense em eqüinos sob condições de campo. Os tratamentos carrapaticidas foram realizados a cada sete dias e divididos em dois módulos: o primeiro com início em abril e término em maio de 2004 (oito banhos e o segundo com início e término em julho de 2004 (cinco banhos, visando atingir as larvas e ninfas do carrapato. Empregou-se a base química piretróide - cipermetrina 0,015%, para banhar os eqüinos. Após o programa de controle houve uma redução de 44,85% da carga parasitária de adultos do carrapato no período de outubro de 2004 a março 2005, e de 59,74%, em fevereiro e março de 2005. Os resultados demonstraram a efetividade do controle estratégico. No entanto, consideram-se importantes fatores operacionais e de gerenciamento que podem interferir na aplicação prática desta tecnologia.The aim of this study was to verify the efficiency of a strategic control program of Amblyomma cajennense in horses under field conditions. Acaricide treatments were applied at seven days intervals and divided in two series, the first one beginning in April 2004 (eight treatments, and the second one beginning in July 2004 (five treatments, aiming to control larvae and nymphs of the tick. A pyrethroid chemical base cypermethrin 0.015% was used for spraying the horses. There was a reduction of 44.85% in the adults infestetion of the tick in the period of October 2004 to March 2005, and 59.74%, in February and March 2005. Results showed the effectiveness of the strategic control. Important administrative and operational procedures can interfere with the practical application of this technology, and should be considered.

  12. Flutuação populacional e sintomas de dano por ácaros (Acari em seringueira no Estado do Mato Grosso, Brasil Population fluctuation and mite (Acari damage symptoms in rubber trees in the State of Mato Grosso, Brazil

    Noeli Juarez Ferla

    2008-04-01

    Full Text Available Apesar da grande importância da cultura da seringueira (Hevea brasiliensis Muell. Arg. para o Brasil, pouco se conhece sobre a flutuação populacional dos ácaros nessa cultura. O objetivo deste trabalho foi estudar a flutuação populacional de ácaros na seringueira no Estado do Mato Grosso, bem como observar a evolução dos sintomas de ataque de tais pragas nessa planta. O estudo foi conduzido em campos de seringueira de Itiquira e Pontes e Lacerda, Estado do Mato Grosso, com seis clones: FX 3864, RRIM 600, IAN 873, IAN 713, PB 260 e PR 255, cujas coletas foram realizadas de agosto de 1998 a julho de 2000. Na safra de 1998/1999, a amostragem foi de 150 folhas de cada um dos estratos basal, mediano e apical, em 10 plantas ao acaso. A metodologia de coleta na safra de 1999/2000 foi alterada devido aos resultados da parcial anterior de cinco folhas do estrato mediano de cada uma das 15 plantas ao acaso. Calacarus heveae Feres foi a espécie mais freqüente em Itiquira e Phyllocoptruta serigueirae Feres em Pontes e Lacerda. A queda prematura das folhas foi observada apenas em Itiquira.Despite the importance of rubber trees (Hevea brasiliensis Muell. Arg. to Brazil, little is known about mite population fluctuation on this crop. The aim of this work was to study the fluctuation of the mite population on rubber trees in the state of Mato Grosso and to observe the evolution of the symptoms caused by their attack. This study was conducted in rubber tree fields at Itiquira and Pontes e Lacerda in the state of Mato Grosso with six clones: FX 3864, RRIM 600, IAN 873, IAN 713, PB 260 and PR 255. Samplings were conducted between August 1998 and July 2000. The 1998/1999 samples consisted of 150 leaves of each of the basal, median and apical strata of 10 plants, randomly taken. The 1999/2000 sampling methodology was changed due the results for the previous partial for five leaves of the median stratum of each of 15 plants randomly taken. Calacarus heveae

  13. Amblyomma rotundatum (Koch, 1844 (Acari: Ixodidae two-host life-cycle on Viperidae snakes Ciclo dioxênico em Amblyomma rotundatum (Koch, 1844 (Acari: Ixodidaeparasitando serpentes da família Viperidae

    Daniel Sobreira Rodrigues

    2010-09-01

    Full Text Available Amblyomma rotundatum is an ixodid tick that infests ectothermic animals and reproduces exclusively by parthenogenesis. This tick has been frequently reported to infest reptiles and amphibians, under natural conditions and sometimes in captivity. It was described in Brazil and several other countries of South, Central and North America. Although many studies have reported aspects of its biology, none of them has used regularly either ophidian as hosts, or controlled temperature, humidity and luminosity for parasitic stages. The objective of this experiment was to study the life cycle of A. rotundatum feeding on Viperidae snakes under room controlled conditions at 27 ± 1 ºC temperature, 85 ± 10% relative humidity and 12:12 hours photoperiod for parasitic stages, and under B.O.D incubator conditions at 27 ± 1 ºC temperature, 85 ± 10% relative humidity and scotophase for non-parasitic stages. The total duration of the life cycle ranged from 56 to 163 days (mean of 105 days. Two-host life cycle was observed for most of the ixodid population studied.Amblyomma rotundatum é um carrapato da família Ixodidae, parasito de animais pecilotérmicos, e que se reproduz exclusivamente por partenogênese. Este carrapato é frequentemente relatado infestando répteis e anfíbios em condições naturais e, às vezes, em animais de cativeiro. Ele já foi relatado no Brasil e em vários outros países das Américas do Sul, Central e do Norte. Embora muitos estudos sobre sua biologia tenham sido publicados, nunca foram utilizados ofídios como hospedeiros e, tão pouco, foram realizados ensaios com os estádios parasitários sob condições controladas de temperatura, umidade e iluminação. O objetivo deste experimento foi estudar o ciclo biológico de A. rotundatum se alimentando em serpentes da família Viperidae sob condições ambientais controladas a 27 ± 1 ºC de temperatura, 85 ± 10% de umidade relativa do ar e 12:12 horas de fotoperíodo para est

  14. Efeito do manejo de plantas daninhas sobre Neoseiulus californicus (Acari:Phytoseiidae) em pomar de macieira Effect of weed management on Neoseiulus californicus (Acari:Phytoseiidae) in apple orchard

    Lino Bittencourt Monteiro; Luiz Belli; Alexander Souza; André Luiz Werner

    2002-01-01

    Avaliou-se a influência do manejo de plantas daninhas sobre o deslocamento de ácaros tetraniquídeos (Panonychus ulmi e Tetranychus urticae) e do ácaro predador Neoseiulus californicus em um pomar de macieira 'Gala', onde foi implantado o controle biológico do ácaro vermelho, P. ulmi, por meio de liberações massais de N. californicus. As parcelas tiveram as plantas daninhas manejadas de três formas: sem manejo, com roçadas manuais e com herbicidas. As populações de ácaros foram avaliadas sobre...

  15. Flutuação populacional e sintomas de dano por ácaros (Acari) em seringueira no Estado do Mato Grosso, Brasil Population fluctuation and mite (Acari) damage symptoms in rubber trees in the State of Mato Grosso, Brazil

    Noeli Juarez Ferla; Gilberto José de Moraes

    2008-01-01

    Apesar da grande importância da cultura da seringueira (Hevea brasiliensis Muell. Arg.) para o Brasil, pouco se conhece sobre a flutuação populacional dos ácaros nessa cultura. O objetivo deste trabalho foi estudar a flutuação populacional de ácaros na seringueira no Estado do Mato Grosso, bem como observar a evolução dos sintomas de ataque de tais pragas nessa planta. O estudo foi conduzido em campos de seringueira de Itiquira e Pontes e Lacerda, Estado do Mato Grosso, com seis clones: FX 38...

  16. Densidade de Neoseiulus californicus (McGregor, 1954 (Acari: Phytoseiidae no controle do ácaro-vermelho da macieira, Fraiburgo-SC Effect of Neoseiulus californicus McGregor (Acari: Phytoseiidae density of on the control of red mite in apple trees

    Lino Bittencourt Monteiro

    2008-12-01

    Full Text Available O controle biológico aplicado de Panonychus ulmi (Koch (Acari: Tetranychidae em macieira iniciou em Vacaria (RS, em 1992, com a multiplicação de Neoseiulus californicus em estufas e liberação nas áreas de ocorrência da praga, fazendo com que o equilíbrio entre ambos os ácaros ocorresse semanas após a liberação. O presente trabalho teve o objetivo de verificar o efeito da densidade de N. californicus a ser liberada em pomar de macieira, Malus domestica (Borkh. Mansf., para o controle do ácaro-vermelho. Foi selecionado um pomar comercial em Fraiburgo (SC e liberados os fitoseídeos nas densidades de 50.000, 100.000 e 150.000 por ha, originados da criação comercial situada na Renar Maçãs (Fraiburgo. O controle do ácaro foi medido através da injúria, devido à alimentação de P. ulmi, e avaliado por meio de uma escala de sintomas de bronzeamento, e do monitoramento de ovos hibernantes em maio, julho e setembro. O tratamento com 150.000 fitoseídeos reduziu a população de ácaros- vermelhos após 16 dias da liberação, enquanto os demais tratamentos necessitaram de 21 dias. A injúria das folhas foi menor no tratamento com 150.000. Os ovos hibernantes em maio, nas parcelas de 50.000 e 100.000, foram, respectivamente, 75% e 69% maiores do que na parcela com 150.000 fitoseídeos. Concluiu-se que a liberação de 150.000 N. californicus foi mais eficiente no controle de P. ulmi no estágio fenológico reprodutivo da macieira.The applied biological control of Panonychus ulmi in apple orchards in Brazil began in Vacaria (RS, in 1992 by rearing Neoseiulus californicus in greenhouses and releasing them into infested areas. The balance between both mites took place weeks after the release. The aim of this work was to evaluate the effect of N. californicus density to be released in apple orchards for the control of the red mite. A commercial orchard in Fraiburgo (SC was chosen and populations of 50,000, 100,000 and 150,000 phytoseiidae

  17. Extratos de plantas no controle de Rhipicephalus (Boophilus microplus (Canestrini, 1887 (Acari: Ixodidae em laboratório Plant extracts in control of Rhipicephalus (Boophilus microplus (Canestrini, 1887 (Acari: Ixodidae in laboratory

    Sônia Maria Forti Broglio-Micheletti

    2009-12-01

    Full Text Available Extratos vegetais foram estudados com o objetivo de avaliar suas eficiências no controle de Rhipicephalus (Boophilus microplus (Canestrini, 1887 em laboratório. Fêmeas ingurgitadas de carrapato foram coletadas de bovinos e mantidas em placas de Petri. Foram utilizados extratos orgânicos alcoólicos 2% (peso/volume de sementes de Annona muricata L. (Annonaceae (graviola; flores de Syzygium malaccensis (L. (Myrtaceae (jambo; folhas de Cymbopogon citratus (DC. Stapf (Poaceae (capim-santo; folhas de Azadirachta indica A. Juss (Meliaceae (nim; e extrato hexânico na mesma concentração de A. indica (sementes. Os grupos-controle foram compostos por fêmeas sem tratamento e fêmeas tratadas com água destilada e esterilizada e dimetilsulfóxido (DMSO a uma concentração de 1%. O extrato de A. muricata apresentou o maior poder acaricida, com eficácia de 100%, seguido dos extratos de S. malaccensis (75 e 59,24% e A. indica (65 e 38,49%. Houve 100% de redução na eclosão das larvas quando se utilizou o extrato de sementes de A. muricata.Plant extracts were studied to evaluate its efficiency in the control of Rhipicephalus (Boophilus microplus (Canestrini, 1887 in laboratory. Engorged female ticks were collected from the cattle, kept in Petri dishes. Organic alcoholic extracts 2% (weight/volume were used: seeds of Annona muricata L. (Annonaceae (soursop; flowers of Syzygium malaccensis (L. (Myrtaceae (iamb, leaves of Cymbopogon citratus (DC. Stapf (Poaceae (holy grass, leaves of Azadirachta indica A. Juss (Meliaceae (neem and hexane extract 2% (weight/volume of A. indica (seeds. The control groups consisted of untreated females and females treated with distilled water and sterile and dimethylsulfoxide (DMSO a concentration of 1%. The extract of A. muricata (seed had the highest mortality with acaricide activity and 100% efficacy followed by extracts of S. malaccensis (75 and 59.24% and A. indica (65 and 38.49%. The seed extract of A. muricata

  18. Life history of Neoseiulus californicus (McGregor, 1954) (Acari: Phytoseiidae) fed with castor bean (Ricinus communis L.) pollen in laboratory conditions.

    Marafeli, P P; Reis, P R; Silveira, E C da; Souza-Pimentel, G C; de Toledo, M A

    2014-08-01

    The predatory mite, Neoseiulus californicus (McGregor, 1954) (Acari: Phytoseiidae) is one of the principal natural enemies of tetranychid mites in several countries, promoting efficient control of those mites in several food and ornamental crops. Pest attacks such as that of the spider mite, Tetranychus urticae Koch, 1836 (Acari: Tetranychidae), is one of the problems faced by farmers, especially in the greenhouse, due to the difficulty of its control with the use of chemicals because of the development of fast resistance making it hard to control it. The objective of this work was to study the life history of the predatory mite N. californicus as a contribution to its mass laboratory rearing, having castor bean plant [Ricinus communis L. (Euphorbiaceae)] pollen as food, for its subsequent use as a natural enemy of T. urticae on a cultivation of greenhouse rosebushes. The studies were carried out in the laboratory, at 25 ± 2°C of temperature, 70 ± 10% RH and a 14 hour photophase. The biological aspects and the fertility life table were appraised. Longevity of 32.9 days was verified for adult females and 40.4 days for males. The intrinsic rate of increase (rm) was 0.2 and the mean generation time (T) was 17.2 days. The population doubled every 4.1 days. The results obtained were similar to those in which the predatory mite N. californicus fed on T. urticae. PMID:25296220

  19. Biologia e exigências térmicas do ácaro-vermelho (Tetranychus ludeni Zacher em folhas de algodoeiro Biology and thermal requirement of Tetranychus ludeni Zacher on cotton leaves

    Carlos Alberto Domingues da Silva

    2002-05-01

    Full Text Available O objetivo deste trabalho foi estudar a biologia e as exigências térmicas de Tetranychus ludeni Zacher (Acari: Tetranychidae em folhas de algodoeiro (Gossypium hirsutum L. r. latifolium (Hutch. Foram utilizadas câmaras climatizadas, ajustadas nas temperaturas de 20, 23, 25, 28 e 30ºC, umidade relativa de 70% e fotófase de 12 horas. Os períodos de desenvolvimento de ovo a adulto variaram de 20,77 (20ºC a 8,50 dias (30ºC, em fêmeas, e de 18,83 (20ºC a 7,75 dias (30ºC, em machos. As temperaturasbase, estimadas pelo método da intersecção de X, para os períodos de desenvolvimento de ovoadulto, foram de 14,05ºC para fêmeas e 13,91ºC para machos, enquanto os valores da constante térmica estimados de acordo com a lei de Réamur foram de 138,34 grausdia, para fêmeas e 130,91 grausdia, para machos. Na temperatura de 30ºC foram observados os maiores valores de razão intrínseca de crescimento (0,418, número de ovos de fêmeas/dia (3,47, fecundidade (61,29 e taxa líquida de reprodução (48,00 e o menor valor para o tempo médio de uma geração (9,27.The objective of this work was to study the biology and thermal requirement of Tetranychus ludeni Zacher (Acari: Tetranychidae on leaves of cotton (Gossypium hirsutum L. r. latifolium (Hutch. Bioclimatic chambers at 20, 23, 25, 28 and 30ºC, relative humidity of 70±10%, and a 12 hours:12 hours (L:D photoperiod were utilized. Development period of eggadult, varied from 20.77 (20ºC to 8.50 days (30ºC, for males and from 18.83 (20ºC to 7.75 days (30ºC, for females. The threshold temperature of development, estimated by X intercession method for development period of eggadult were 14.05ºC, for females and 13.91ºC, for males. While those values of thermal constant estimated by Réamur law were 138.34 daydegrees for females and 130.91 daydegrees for males. At 30ºC of temperature, the highest values of the intrinsic rate of increase (0.418, number of eggs/females/day (3.47, fecundity

  20. Carrapatos (Acari: Ixodidae em mamíferos silvestres do Parque Nacional da Serra da Canastra e arredores, Minas Gerais, Brasil

    Thiago Fernandes Martins

    2015-02-01

    Full Text Available O presente estudo relata ixodídeos em mamíferos silvestres no Parque Nacional da Serra da Canastra e arredores, no estado de Minas Gerais, Brasil. De julho de 2005 a junho de 2014, foram coletadas 58 larvas, 133 ninfas e 43 carrapatos adultos em 40 eventos de captura de mamíferos de vida livre do Parque e arredores. Nos hospedeiros da ordem Carnivora, foram identificados Amblyomma ovale Koch, 1844, Amblyomma sculptum Berlese, 1888, Amblyomma tigrinum Koch, 1844, Dermacentor nitens Neumann, 1897 e Rhipicephalus microplus (Canestrini, 1888. Em hospedeiros da ordem Xenarthra, foram identificados espécimes de Amblyomma pseudoconcolor Aragão, 1908 e A. sculptum. Este estudo relata os primeiros registros dos ixodídeos D. nitens e R. microplus parasitando Lycalopex vetulus, e A. ovale em Leopardus tigrinus no país. Os achados do presente estudo indicam a proximidade entre os animais domésticos e silvestres causada pela expansão agropecuária no território brasileiro

  1. Atividade do acaricida etoxazol sobre a mortalidade e reprodução do ácaro-da-leprose Brevipalpus phoenicis (Geijskes (Acari: Tenuipalpidae, em citros

    Fernando Juari Celoto

    2010-12-01

    Full Text Available O objetivo deste trabalho foi avaliar a atividade do acaricida etoxazol, no controle e reprodução do ácaro B. phoenicis. Para tanto, foram demarcadas com cola adesiva arenas de cinco centímetros de diâmetro em frutos de citros com alta infestação do ácaro. O ensaio foi delineado em parcelas inteiramente casualizadas, com oito tratamentos e quatro repetições. Em cada arena foram contados o número de ácaros adultos, jovens e ovos. Os tratamentos constaram dos seguintes acaricidas e doses em g i.a./100 L de água: etoxazol 110 SC (1,1; 1,65; 2,75 e 5,5; hexitiazoxi 500 PM (0,75; flufenoxuron 100 CE (3; cihexatina 500 PM (25, aplicados diretamente sobre as arenas. Os frutos foram mantidos em câmara de germinação tipo BOD. com temperatura de 25 ± 2 ºC e fotofase de 12 horas. Diariamente, foram contados o número de ácaros adultos, jovens e ovos, com auxílio de microscópio esteroscópio. Os parâmetros avaliados foram a atividade ovicida, esterilização de fêmeas e efeito sobre formas jovens. Constatou-se que o etoxazol provocou mortalidade de formas jovens do ácaro-da-leprose superior a 95%, nas doses a partir de 1,1 g i.a. /100 L de água. Ovos tratados com etoxazol, nas doses a partir de 1,65 g i.a. /100 L de água, apresentaram inviabilidade média de 60%. O etoxazol apresentou efeito esterilizante sobre fêmeas nas doses a partir de 2,75 g i.a./100 L de água, inviabilizando 95% dos ovos.

  2. Uropodina species from Vietnam (Acari: Mesostigmata)

    Kontschán, J.; Starý, Josef

    -, č. 2807 (2011), s. 1-28. ISSN 1175-5326 Institutional research plan: CEZ:AV0Z60660521 Keywords : Acari * Uropodina * new species Subject RIV: EH - Ecology, Behaviour Impact factor: 0.927, year: 2011

  3. Fatores que afetam artrópodes associados a cinco acessos de ginseng-brasileiro (Pfaffia glomerata em Montes Claros, Estado de Minas Gerais = Factors affecting arthropods associated with five accessions of Brazilian ginseng (Pfaffia glomerata in Montes Claros, Brazil

    Germano Leão Demolin Leite

    2008-01-01

    Full Text Available O objetivo deste trabalho foi identificar artrópodes associados a cinco acessos ('NDS', 'COVB', 'NAT', 'ROST' e 'GSD1' de Pfaffia glomerata, bem como o efeito de clima, dossel, face foliar, tricomas e inimigos naturais sobre a entomofauna. Dos artrópodes observados, Aphis gossypii (Hemiptera: Aphididae, Diabrotica speciosa (Coleoptera: Chrysomelidae e Tetranychus ludeni (Acari: Tetranychidae apresentaram maior densidade populacional. Dos inimigos naturais observados, ácaros predadores estiveram associados ao ácaro branco Polyphagotarsonemus latus (Acari: Tarsonemidae, e um complexo de aranhas a besouros desfolhadores, pulgões e cicadelídeos. Observaram-se correlações significativas diretas múltiplas e lineares da população de A. gossypii com maior densidade de Crematogaster sp.(Hymenotera: Formicidae (protocooperação e correlação negativa com densidade de Cycloneda sanguinea (Coleoptera: Coccinelidae e a temperatura do ar. Foi observado maior ataque de ácaros T. ludeni, T. urticae (Tetranychidae e P. latus, em períodos de menor umidaderelativa. Observou-se maior densidade populacional de A. gossypii, nos acessos 'ROST' e 'NAT' e de T. ludeni ao acesso 'ROST'. O acesso 'GSD1' foi o mais danificado por besouros desfolhadores. Pulgões e ácaros preferiram alimentar-se na face abaxial das folhas apicais,enquanto D. speciosa apresentou preferência pela face adaxial do terço mediano do dossel. Tricomas (100% tectores não afetaram os artrópodes.The objective of this study was to identify arthropods associated with five accessions ('NDS', 'COVB', 'NAT', 'ROST' and 'GSD1' of Pfaffia glomerata and the effect of weather, canopy height, foliar face, trichomes, and naturals enemies over the entomofauna. The arthropods Aphis gossypii, Diabrotica speciosa, and Tetranychus ludeni had the highest population densities. Among naturals enemies, there were predator mites associated with the broad mitePolyphagotarsonemus latus, and the spiders

  4. INTEGRACIÓN DE CONTROL BIOLÓGICO Y DE PRODUCTOS ALTERNATIVOS CONTRA Tetranychus urticae (ACARI: TETRANYCHIDAE INTEGRATION OF BIOLOGICAL CONTROL AND ALTERNATIVE PRODUCTS AGAINST Tetranychus urticae (ACARI: TETRANYCHIDAE

    Alberto Soto

    Full Text Available Tetranychus urticae es una importante plaga asociada al cultivo de fresa debido a las altas densidades poblacionales alcanzadas y a los daños ocasionados a las plantas. Los productores utilizan acaricidas en grandes volúmenes sin cumplir los periodos de carencia, ocasionando residuos tóxicos en los frutos, desarrollo de poblaciones resistentes a los productos, destrucción de organismos benéficos, intoxicación de mamíferos y contaminación del medio ambiente. Por lo tanto, es necesario generar otras alternativas de manejo de este ácaro plaga. El objetivo de este trabajo fue evaluar, mediante el análisis conjunto de la concentración letal y subletal, la acción tóxica de productos comerciales a base de nim sobre los ácaros T. urticae y Phytoseiulus macropilis. Fueron controladas poblaciones de T. urticae en plantas de fresa cuando se emplearon concentraciones correspondientes a CL95 de los productos alternativos a base de nim, y también cuando se utilizaron concentraciones inferiores. La aplicación de los productos a base de nim, Neem Pro, Organic Neem y Natuneem en la CL95 y cuando la tasa instantánea de crecimiento poblacional ri=0,1 para el predador representan una alternativa viable para el control del ácaro fitófago T. urticae.Tetranychus urticae is an important pest associated with strawberry, due to its high densities and plant damage. Farmers use acaricides in large volumes without complying with the window between the last application and harvest, resulting on toxic residues in fruits, as well as pest populations resistant to pesticides, decrement of beneficial organisms, mammalian toxicity and environmental pollution. Therefore, it is necessary to generate new alternatives for the control of this mite. The aim of this work was to evaluate the toxic effect of commercial neem-based products on the mites T. urticae and Phytoseiulus macropilis, through a combined analysis using both, lethal and sublethal concentrations. Tetranychus urticae populations were controlled in strawberry plants when neem was used at or below LC95 concentrations. The application of the neem-based products, Neem Pro, Organic Neem and Natuneem at CL95, and when the instantaneous predator’s rate of population increase ri = 0.1, represents a viable alternative to be considered for the control of the phytophagous mite T. urticae.

  5. Lâminas de irrigação e sombreamento na produção de biomassa de Acariçoba (Hydrocotyle umbellata L.)

    Alves, N. M.; M.D.B. LIMA; J.R. Paula; G.A. SIMON

    2015-01-01

    RESUMO:A acariçoba é uma planta utilizada pela medicina ayurvédica e em cosméticos por possuir propriedades rejuvenescedoras e revitalizante do sistema nervoso e cérebro. Com objetivo de avaliar os caracteres agronômicos e o teor de flavonóides totais de acariçoba em resposta a aplicação de lâminas distintas de irrigação e sombreamento, foi conduzido um experimento em área localizada nas coordenadas 17º 47' 53'' latitude (S); longitude (W) - 51º 55' 53'', com altitude média de 648m, no períod...

  6. Typhlodromus pyri and Euseius finlandicus (Acari: Phytoseiidae) as potential biocontrol agents against spider mites (Acari: Tetranychidae) inhabiting willows: laboratory studies on predator development and reproduction on four diets.

    Puchalska, Ewa K; Kozak, Marcin

    2016-01-01

    Typhlodromus pyri Scheuten and Euseius finlandicus (Oudemans) are important predators of phytophagous mites. The present laboratory study aimed to determine whether both species can develop and reach maturity feeding on spider mites occurring on willows, i.e., Schizotetranychus schizopus (Zacher), Schizotetranychus garmani Pritchard & Baker, and Tetranychus urticae Koch, and on Brassica napus L. pollen. The predators' development, reproduction and demographic parameters were significantly affected by diet. The data suggest that rape pollen can be useful in mass rearing of E. finlandicus but is completely unsuitable as alternative food for T. pyri. Short development time and high values of population parameters achieved by T. pyri feeding on larvae and protonymphs of S. schizopus and by E. finlandicus feeding on juvenile stages of S. garmani indicate great suitability of these preys as food for the phytoseiids, and make both predatory species promising biocontrol agents in spider mite control on willows. PMID:26530991

  7. Criterios para el manejo de tetranychus urticae koch (acari: tetranychidae) con el ácaro depredador amblyseius (neoseiulus) sp. (acari: phytoseiidae) en cultivos de rosas

    Forero, Gabriel; Rodríguez, Martha; Cantor, Fernando; Rodríguez,Daniel; Cure, José Ricardo

    2010-01-01

    En cultivos de rosas en la Sabana de Bogotá, se ha registrado Amblyseius sp. como un ácaro depredador de Tetranychus urticae. Con el fin de evaluar el potencial de este ácaro en el manejo de T. urticae, se comparó en cultivos comerciales de rosa, bajo el sistema de agobio, la efectividad de liberaciones de Amblyseius sp. frente a la aplicación de productos de síntesis química, teniendo en cuenta los siguientes criterios de evaluación: presencia-ausencia del ácaro fitófago, porcentaje d...

  8. Purification and partial characterization of glutathione S-transferases from three field populations of Panonychus citri (Acari: Tetranychidae).

    Niu, Jin-Zhi; Dou, Wei; Wang, Bao-Jun; Zhang, Guo-Na; Zhang, Rui; Yin, Yi; Wang, Jin-Jun

    2012-02-01

    Glutathione S-transferases (GSTs) play central roles in phase II detoxification of both xenobiotics (drugs, insecticides, and herbicides) and endogenous compounds in almost all living organisms. In this study, we successfully purified the GSTs from the citrus red mite, Panonychus citri, by affinity chromatography on Glutathione Sepharose 4B and compared the biochemical characterizations of the purified GSTs from three field populations [beibei (BB), wanzhou (WZ), and zhongxian (ZX)]. SDS-PAGE revealed that the molecular weight of GSTs from three populations consisted of two subunits of 27.3 and 26.1 kDa. The specific activity of the purified GSTs from the WZ and ZX populations was increased 1.5- and 3.8-fold, respectively, compared with the BB population. Accordingly, the pyridaben susceptibility of WZ and ZX populations was less compared with BB population. Kinetic analyses showed that the WZ and ZX populations had higher substrate specificity compared with the BB population based on the values of k (cat) and k (cat) /K (m) to both reduced glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB). The in vitro inhibition studies of GSTs indicated that the I (50) values of pyridaben from WZ and ZX populations of P. citri expressed 1.6- and 4.4-fold decreases, respectively, compared to the I (50) value of pyridaben from the BB population. In conclusion, all evidence suggested that the purified GSTs may partially contribute to the susceptibility of acaricide pyridaben in field populations of P. citri. PMID:21979304

  9. 60Co-γ Irradiation Affects the Enzymatic Antioxidant System of the Citrus Red Mite Panonychus citri (Acari: Tetranychidae)

    Ke Zhang; Zhibin Li; Shaowen Zhu; Qunfang Weng

    2014-01-01

    Radio-(60Co), which emits γ rays, has been used worldwide in pest control. The aim of the present study was to analyze the effect of effective-low-power 60Co-γ irradiation on the enzymatic antioxidant system of the citrus red mite Panonychus citri. One day old female adults were exposed to 0.4 kGy 60Co-γ irradiation and on the, 6th h, 1st day, 2nd day, and 5th day post treatment, the mites were euthanized for biochemical analysis. The activity of superoxide dismutase (SOD), catalase (CAT), p...

  10. Antioxidant responses of citrus red mite, Panonychus citri (McGregor) (Acari: Tetranychidae), exposed to thermal stress.

    Yang, Li-Hong; Huang, Hai; Wang, Jin-Jun

    2010-12-01

    Relatively low or high temperatures are responsible for a variety of physiological stress responses in insects and mites. Induced thermal stress was recently associated with increased reactive oxygen species (ROS) generation, which caused oxidative damage. In this study, we examined the time-related effect of the relatively low (0, 5, 10, and 15 °C) or high (32, 35, 38, and 41 °C) temperatures on the activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), peroxidases (POX), and glutathione-S-transferase (GST), and the total antioxidant capacity (TEAC) of the citrus red mite, Panonychus citri (McGregor). The malondialdehyde (MDA) concentration, as a marker of lipid peroxidation in organisms, was also measured in the citrus red mite under thermal stress conditions. Results showed that SOD and GST activities were significantly increased and play an important role in the process of antioxidant response to thermal stress. Lipid peroxidation levels increased significantly (P<0.001) and changed in a time-dependent manner. CAT and POX activity, as well as TEAC, did not vary significantly and play a minor role to remove the ROS generation. These results suggest that thermal stress leads to oxidative stress and antioxidant enzymes play an important role in reducing oxidative damage in the citrus red mite. PMID:20709071

  11. (60)Co-γ irradiation affects the enzymatic antioxidant system of the citrus red mite Panonychus citri (Acari: Tetranychidae).

    Zhang, Ke; Li, Zhibin; Zhu, Shaowen; Weng, Qunfang

    2014-01-01

    Radio-(60Co), which emits γ rays, has been used worldwide in pest control. The aim of the present study was to analyze the effect of effective-low-power 60Co-γ irradiation on the enzymatic antioxidant system of the citrus red mite Panonychus citri. One day old female adults were exposed to 0.4 kGy 60Co-γ irradiation and on the, 6th h, 1st day, 2nd day, and 5th day post treatment, the mites were euthanized for biochemical analysis. The activity of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), phenoloxidase (PO) and acetylocholinesterase (AchE) were investigated. POD and CAT activities, as well as SOD were higher in the irradiated mites. We found that exposure to 60Co-γ radiation resulted in increased activities of SOD, CAT, POD and decreased AchE activity. When the recovery time lasted till the 5th day, the activities of POD and PO were significantly lower than the control, whereas the SOD, CAT and AchE activities returned to control levels. Cells possess protein repair pathways to rescue oxidized proteins and restore their functions, but if these repair processes fail, oxidized proteins may become cytotoxic. Our results confirm the hypothesis that low dosages of 60Co-γ irradiation increase the level of oxidative stress in P. citri adults in a short time, causing meanwhile damage and sterility. The results of this study also indicate that stress caused by exposure to irradiation could inhibit the cholinergic system in P. citri. PMID:24853454

  12. Analysis of transcriptome differences between resistant and susceptible strains of the citrus red mite Panonychus citri (Acari: Tetranychidae.

    Bin Liu

    Full Text Available BACKGROUND: The citrus red mite is a worldwide citrus pest and a common sensitizing allergen of asthma and rhinitis. It has developed strong resistance to many registered acaricides, However, the molecular mechanisms of resistance remain unknown. we therefore used next generation sequencing technology to investigate the global transcriptomes between resistant strains and susceptible strains. RESULTS: We obtained 34,159, 30,466 and 32,217 unigenes by assembling the SS reads, RS reads and SS&RS reads respectively. There are total 17,581 annotated unigenes from SS&RS reads by BLAST searching databases of nr, the Clusters of Orthologous Groups (COGs and Kyoto Encyclopedia of Genes and Genomes (KEGG with an E-value ≤ 1e-5, in which 7,075 unigenes were annotated in the COG database, 12, 712 unigenes were found in the KEGG database and 3,812 unigenes were assigned to Gene ontology (GO. Moreover, 2,701 unigenes were judged to be the differentially expressed genes (DEGs based on the uniquely mapped reads. There are 219 pathways in all annotated unigenes and 198 pathways in DEGs that mapped to the KEGG database. We identified 211 metabolism genes and target genes related to general insecticide resistance such as P450 and Cytochrome b, and further compared their differences between RS and SS. Meanwhile, we identified 105 and 194 genes related to growth and reproduction, respectively, based on the mode of action of Hexythiazox. After further analyses, we found variation in sequences but not in gene expression related to mite growth and reproduction between different strains. CONCLUSION: To our knowledge, this is the first comparative transcriptome study to discover candidate genes involved in phytophagous mite resistance. This study identified differential unigenes related to general pesticide resistance and organism growth and reproduction in P. citri. The assembled, annotated transcriptomes provide a valuable genomic resource for further understanding the molecular basis of resistance mechanisms.

  13. Analysis of Transcriptome Differences between Resistant and Susceptible Strains of the Citrus Red Mite Panonychus citri (Acari: Tetranychidae)

    Bin Liu; Gaofei Jiang; Yunfei Zhang; Junli Li; Xiaojiao Li; Jiansu Yue; Fei Chen; Haoqiang Liu; Hongjun Li; Shiping Zhu; Jinjun Wang; Chun Ran

    2011-01-01

    BACKGROUND: The citrus red mite is a worldwide citrus pest and a common sensitizing allergen of asthma and rhinitis. It has developed strong resistance to many registered acaricides, However, the molecular mechanisms of resistance remain unknown. we therefore used next generation sequencing technology to investigate the global transcriptomes between resistant strains and susceptible strains. RESULTS: We obtained 34,159, 30,466 and 32,217 unigenes by assembling the SS reads, RS reads and SS&RS...

  14. Laboratory bioassay of Beauveria bassiana against Tetranychus urticae (Acari: Tetranychidae) on leaf discs and potted bean plants.

    Ullah, Mohammad Shaef; Lim, Un Taek

    2015-03-01

    Use of the mycopathogen Beauveria bassiana (strain GHA), marketed as BotaniGard(®) ES, was evaluated as a plant protection strategy against the spider mite Tetranychus urticae Koch, which is considered one of the most economically important and cosmopolitan pests of many crops. Tetranychus urticae were treated with four concentrations of conidia (1 × 10(5), 1 × 10(6), 1 × 10(7), or 1 × 10(8) conidia/ml), and virulence was assessed on mites held at four relative humidity levels (35, 55, 75, and 95 ± 2 % RH) at 25 ± 1 °C. At 1 × 10(8) spores/ml, the LT50 value was 9.7 h at 95 % RH, which was significantly lower than values for other RH levels. At 1 × 10(7) spores/ml, the LT50 value was 43.8 h at 95 % RH, which was significantly different from values at 55 and 35 % RH. The efficacy of B. bassiana product was also verified on mites infesting potted bean plants with a concentration of 1 × 10(8) spores/ml. In double spray treatment where applications were made 2 × on days 5 and 10 after mite infestation, the nymphal and adult population of T. urticae were reduced to zero on days 20 and 15, respectively. With a single spray on day 5, the nymphal population was also greatly reduced, but increased rapidly after day 20. Single and double sprays with B. bassiana reduced leaf damage as measured by image analysis by 33 and 94 % compared to no treatment, respectively. These results suggest that 1 × 10(8) spores/ml was the most effective dose and that two applications, at a 5-day interval, provided control of T. urticae in our laboratory assay. PMID:25500970

  15. Effects of Nitrogen Fertilization on Potato Leafhopper (Hemiptera: Cicadellidae) and Maple Spider Mite (Acari: Tetranychidae) on Nursery-Grown Maples.

    Prado, Julia; Quesada, Carlos; Gosney, Michael; Mickelbart, Michael V; Sadof, Clifford

    2015-06-01

    Although leaf nitrogen (N) has been shown to increase the suitability of hosts to herbivorous arthropods, the responses of these pests to N fertilization on susceptible and resistant host plants are not well characterized. This study determined how different rates of N fertilization affected injury caused by the potato leafhopper (Empoasca fabae Harris) and the abundance of maple spider mite (Oligonychus aceris (Shimer)) on 'Red Sunset' red maple (Acer rubrum) and 'Autumn Blaze' Freeman maple (Acer×freemanii) during two years in Indiana. N fertilization increased leaf N concentration in both maple cultivars, albeit to a lesser extent during the second year of the study. Overall, Red Sunset maples were more susceptible to E. fabae injury than Autumn Blaze, whereas Autumn Blaze maples supported higher populations of O. aceris. Differences in populations of O. aceris were attributed to differences between communities of stigmaeid and phytoseiid mites on each cultivar. Injury caused by E. fabae increased with N fertilization in a dose-dependent manner in both cultivars. Although N fertilization increased the abundance of O. aceris on both maple cultivars, there was no difference between the 20 and 40 g rates. We suggest the capacity of N fertilization to increase O. aceris on maples could be limited at higher trophic levels by the community of predatory mites. PMID:26470249

  16. Effects of spirodiclofen on the reproductive potential of two-spotted spider mite (Acari: Tetranychidae ovipositing females

    Marčić D.

    2009-01-01

    Full Text Available A laboratory bioassay was conducted to evaluate the effects of spirodiclofen on the survival and reproduction of young and mated females of two-spotted spider mite (Tetranychus urticae Koch. The females were sprayed with a series of acaricide concentrations (96, 48, 24, 12, and 6 mg/l 24-30 h after adult emergence, i.e., at the age most likely to exhibit dispersal behavior and close to their reproductive maximum. The proportions of T. urticae females that survived treatment without symptoms of poisoning were concentration-dependent, ranging between 0.41 and 0.88 (0.96 in the control. With the exception of females that survived 6 mg/l, fecundity of the treated female mites was strongly affected during the exposure, compared to the control. The mean daily fecundity (EL and mean daily fertility (EH of surviving females, transferred daily to new leaf disks over the following five days, significantly decreased as spirodiclofen concentrations increased. In treatments with 6 mg/l and 12 mg/l, only the latter concentration significantly reduced both EL and EH, compared to the control. In females that survived 24 mg/l and 48 mg/l, these life history parameters were reduced by over 90%, while treatment with 96 mg/l completely terminated egg-laying. The treated females lived for a significantly shorter time than untreated ones, with the exception of females that survived 6 mg/l. Compared to the control females, gross fecundity (GL and gross fertility (GH of the treated females were strongly reduced on the first and second day; from the third day onward, females treated with the lowest concentrations achieved marked recovery, their GL and GH going even above the values in the control. However, net fecundity (NL and net fertility (NH of all treated females decreased considerably throughout the trial, indicating that survival rates of these females were lower, compared to the control. Calculated as total sums of gross and net daily schedules within five days, fecundity and fertility significantly decreased as spirodiclofen concentration increased. The two lowest concentrations failed to achieve a significant reduction of GL, while GH, NL, and NH were significantly lower than control values starting with the females treated with 6 mg/L. A high percentage of unhatched eggs, especially during the initial two days after treatment (35-100%, further contributed to the significant reduction in fertility of the females treated with spirodiclofen. All concentrations of spirodiclofen significantly reduced the instantaneous rate of increase. Regression analysis showed a linear population decline with increased acaricide concentrations (y = 1.13 - 0.24x; R2 = 0.91, p < 0.05.

  17. Microsatellites reveal a strong subdivision of genetic structure in Chinese populations of the mite Tetranychus urticae Koch (Acari: Tetranychidae

    Sun Jing-Tao

    2012-02-01

    Full Text Available Abstract Background Two colour forms of the two-spotted spider mite (Tetranychus urticae Koch coexist in China: a red (carmine form, which is considered to be native and a green form which is considered to be invasive. The population genetic diversity and population genetic structure of this organism were unclear in China, and there is a controversy over whether they constitute distinct species. To address these issues, we genotyped a total of 1,055 individuals from 18 red populations and 7 green populations in China using eight microsatellite loci. Results We identified 109 alleles. We found a highly significant genetic differentiation among the 25 populations (global FST = 0.506, global FST {ENA} = 0.473 and a low genetic diversity in each population. In addition, genetic diversity of the red form mites was found to be higher than the green form. Pearson correlations between statistics of variation (AR and HE and geographic coordinates (latitude and longitude showed that the genetic diversity of the red form was correlated with latitude. Using Bayesian clustering, we divided the Chinese mite populations into five clades which were well congruent with their geographic distributions. Conclusions Spider mites possess low levels of genetic diversity, limit gene flow between populations and significant and IBD (isolation by distance effect. These factors in turn contribute to the strong subdivision of genetic structure. In addition, population genetic structure results don't support the separation of the two forms of spider mite into two species. The morphological differences between the two forms of mites may be a result of epigenetic effects.

  18. Survival and Reproductive Strategies in Two-Spotted Spider Mites: Demographic Analysis of Arrhenotokous Parthenogenesis of Tetranychus urticae (Acari: Tetranychidae).

    Tuan, Shu-Jen; Lin, Yung-Hsiang; Yang, Chung-Ming; Atlihan, Remzi; Saska, Pavel; Chi, Hsin

    2016-04-01

    Tetranychus urticae Koch is a cosmopolitan pest whose rapid developmental rate enables it to produce colonies of thousands of individuals within a short time period. When a solitary virgin female colonizes a new host plant, it is capable of producing male offspring through the arrhenotokous parthenogenesis; once her sons mature, oedipal mating occurs and the female will produce bisexual offspring. To analyze the effect of arrhenotokous reproduction on population growth, we devised and compared separate life tables for arrhenotokous and bisexual populations of T. urticae using the age-stage, two-sex life table theory. For the cohort with bisexual reproduction, the intrinsic rate of increase (r), finite rate (λ), net reproductive rate (R0), and mean generation time (T) were 0.2736 d(−1), 1.3146 d(−1), 44.66 offspring, and 13.89 d, respectively. Because only male eggs were produced during the first 8 d of the oviposition period and the cohort would soon begin bisexual reproduction, it would be theoretically wrong to calculate the population parameters using the survival rate and fecundity of an arrhenotokous cohort. We demonstrated that the effect of arrhenotokous reproduction could be accurately described and evaluated using the age-stage, two-sex life table. We also used population projection based on life table data, quantitatively showing the effect that arrhenotokous reproduction has on the growth potential and management of T. urticae. PMID:26743215

  19. An analysis of potential resistance of the phytophagous mite, Tetranychus urticae Koch (Acari: Tetranychidae to four botanical pesticides

    Attia, S.

    2015-01-01

    Full Text Available Description of the subject. Synthetic acaricides have been widely used to manage Tetranychus urticae. Due to the excessive use of biocide and the associated problems of pesticide resistance and environmental pollution, there is an increasing demand for sustainable, environmentally-friendly control methods. Among the current alternative strategies aimed at decreasing the pest populations, the pesticides based on plant extracts are currently one of the most promising methods. Essential oils with acaricidal properties have been categorized as green pesticides because they are biodegradable and predominantly non-toxic to vertebrates. Objectives. With an aim to reduce the use of synthetic pesticides, they represent a promising approach for eco-chemical control of mites. Method. The aim of the present work was to analyze the risk of resistance emergence of T. urticae to repeated treatments with four plant extracts: Deverra scoparia Coss. & Durieu (Araliales: Apiaceae, Hertia cheirifolia (L. Kuntze (Asterales: Ateraceae, Santolina africana Jord. & Fourr. (Asterales: Asteraceae essential oils and garlic distillate Allium sativum L. (Asparagales: Alliaceae after 20 generations. Results. Repeated treatments with S. africana essential oil during 20 generations did not provoke an emergence of resistance while a low development of resistance was observed with H. cheirifolia, A. sativum and D. scoparia extracts. Conclusions. The efficacy of these extracts against the two spotted spider mite and their low development of resistance make them a promising use for pest management.

  20. The influence od different maize varieties and treatments on the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae)

    Svobodová, Zdeňka; Zemek, Rostislav; Habuštová, Oxana; Hussein, Hany; Sehnal, František

    Vol. 93. Darmstad : Federal Research Center for Cultivated Plants, 2013, s. 119-123. ISBN 978-92-9067-272-2. [Proceedings of the Third Working Group Meeting. Český Krumlov (CZ), 13.09.2011-16.09.2011] R&D Projects: GA MZe QH91093 Grant ostatní: projekt MOBITAG(CZ) REGPOT-2008-1, GA 229518 Institutional support: RVO:60077344 Keywords : Tetranychus urticae * transgenic plants * MON 88017 Subject RIV: GF - Plant Pathology, Vermin, Weed, Plant Protection

  1. Reproductive performance of seven strains of the tomato red spider mite Tetranychus evansi (Acari: Tetranychidae) at five temperatures

    Gotoh, T.; Sugimoto, N.; Pallini, A.; Knapp, M.; Hernandez-Suarez, E.; Ferragut, F.; Ho, Sin C; Migeon, A.; Navajas, M.; Nachman, G.

    2010-01-01

    The tomato red spider mite Tetranychus evansi Baker et Pritchard occurs on solanaceous plants, and causes serious damage to a variety of crops in Africa and Europe. In 2001 this species was also found in Japan, on nightshade (Solanum nigrum L.), and its invasion to solanaceous of agricultural...... importance is feasible. To evaluate its potential severity as a pest, the present study assessed the life-history parameters, such as the rate of development and the intrinsic rate of natural increase (r (m)), on S. nigrum for T. evansi collected on seven sites worldwide. Increasing temperatures between 15...

  2. A comparative study of development and demographic parameters of Tetranychus merganser and Tetranychus kanzawai (Acari: Tetranychidae) at different temperatures

    Ullah, M. S.; Moriya, D.; Badii, M.H.; Nachman, Gøsta Støger; Gotoh, T.

    2011-01-01

    We investigated the effect of temperature on development and demographic parameters such as the intrinsic rate of natural increase (rm) of the two spider mite species Tetranychus merganser Boudreaux and T. kanzawai Kishida at eleven constant temperatures ranging from 15 to 40C at intervals of 2.5...

  3. Repellent effects of various cherry tomato accessions on the two-spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae).

    Lucini, T; Resende, J T V; Oliveira, J R F; Scabeni, C J; Zeist, A R; Resende, N C V

    2016-01-01

    Several studies have been conducted on resistance sources to improve the genetic resistance of farm-grown tomatoes to arthropod pests, including phytophagous mites. In the present study, we evaluate the behavior of the two-spotted spider mite on different cherry tomato accessions to identify possible sources of resistance (repellent effect) to this pest. Sixty-four accessions of cherry tomatoes, Solanum lycopersicum var. cerasiforme (Dunal), were tested. In addition, a commercial cultivar of cherry tomato cv. Sweet Grape (susceptible pattern) and the wild tomato accession Solanum pennellii Correll LA-716 (multiple pest resistance) were evaluated as well. The distance traveled by mites on the leaflet surface over time varied largely among cherry tomato accessions. The wild genotype, S. pennellii LA-716, showed the smallest traveled distance on the leaflet surface (0.8 to 1.1 mm over time), and the variety cv. Sweet Grape was one of the genotypes with highest traveled distance (16.2 to 16.4 mm over time). The cherry tomato accessions 2298-42, RVTC-03, and 6889-53 showed a decrease in the traveled distance by mites over time, similar to that as observed in the wild tomato accession LA716. These accessions showed mite repellence levels similar to those of the wild genotype and may, therefore, be good candidates for breeding programs dealing with resistance to mites. PMID:27050983

  4. Uncertainties in predicting species distributions under climate change: a case study using Tetranychus evansi (Acari: Tetranychidae), a widespread agricultural pest

    Meynard, Christine N.; Alain Migeon; Maria Navajas

    2013-01-01

    Many species are shifting their distributions due to climate change and to increasing international trade that allows dispersal of individuals across the globe. In the case of agricultural pests, such range shifts may heavily impact agriculture. Species distribution modelling may help to predict potential changes in pest distributions. However, these modelling strategies are subject to large uncertainties coming from different sources. Here we used the case of the tomato red spider mite (Tetr...

  5. Uncertainties in predicting species distributions under climate change: a case study using Tetranychus evansi (Acari: Tetranychidae), a widespread agricultural pest.

    Meynard, Christine N; Migeon, Alain; Navajas, Maria

    2013-01-01

    Many species are shifting their distributions due to climate change and to increasing international trade that allows dispersal of individuals across the globe. In the case of agricultural pests, such range shifts may heavily impact agriculture. Species distribution modelling may help to predict potential changes in pest distributions. However, these modelling strategies are subject to large uncertainties coming from different sources. Here we used the case of the tomato red spider mite (Tetranychus evansi), an invasive pest that affects some of the most important agricultural crops worldwide, to show how uncertainty may affect forecasts of the potential range of the species. We explored three aspects of uncertainty: (1) species prevalence; (2) modelling method; and (3) variability in environmental responses between mites belonging to two invasive clades of T. evansi. Consensus techniques were used to forecast the potential range of the species under current and two different climate change scenarios for 2080, and variance between model projections were mapped to identify regions of high uncertainty. We revealed large predictive variations linked to all factors, although prevalence had a greater influence than the statistical model once the best modelling strategies were selected. The major areas threatened under current conditions include tropical countries in South America and Africa, and temperate regions in North America, the Mediterranean basin and Australia. Under future scenarios, the threat shifts towards northern Europe and some other temperate regions in the Americas, whereas tropical regions in Africa present a reduced risk. Analysis of niche overlap suggests that the current differential distribution of mites of the two clades of T. evansi can be partially attributed to environmental niche differentiation. Overall this study shows how consensus strategies and analysis of niche overlap can be used jointly to draw conclusions on invasive threat considering different sources of uncertainty in species distribution modelling. PMID:23799105

  6. Spatial Ecology and Remote Sensing in the Precision Management of Tetranychus urticae (Acari: Tetranychidae)in Peanut

    Holden, Erin

    2002-01-01

    The twospotted spider mite (TSSM), Tetranychus urticae Koch, is a common polyphagous pest in peanut agroecosystems. The mite has caused serious economic losses to peanut farmers in the Virginia-Carolina area, where approximately 20% of the peanuts are produced annually in the United States. Peanut farmers depend on pesticides to control mite populations. Because TSSM has developed resistance to many acaricides and there are restrictions on the use of pesticides, an alternative approach, su...

  7. Uncertainties in predicting species distributions under climate change: a case study using Tetranychus evansi (Acari: Tetranychidae, a widespread agricultural pest.

    Christine N Meynard

    Full Text Available Many species are shifting their distributions due to climate change and to increasing international trade that allows dispersal of individuals across the globe. In the case of agricultural pests, such range shifts may heavily impact agriculture. Species distribution modelling may help to predict potential changes in pest distributions. However, these modelling strategies are subject to large uncertainties coming from different sources. Here we used the case of the tomato red spider mite (Tetranychus evansi, an invasive pest that affects some of the most important agricultural crops worldwide, to show how uncertainty may affect forecasts of the potential range of the species. We explored three aspects of uncertainty: (1 species prevalence; (2 modelling method; and (3 variability in environmental responses between mites belonging to two invasive clades of T. evansi. Consensus techniques were used to forecast the potential range of the species under current and two different climate change scenarios for 2080, and variance between model projections were mapped to identify regions of high uncertainty. We revealed large predictive variations linked to all factors, although prevalence had a greater influence than the statistical model once the best modelling strategies were selected. The major areas threatened under current conditions include tropical countries in South America and Africa, and temperate regions in North America, the Mediterranean basin and Australia. Under future scenarios, the threat shifts towards northern Europe and some other temperate regions in the Americas, whereas tropical regions in Africa present a reduced risk. Analysis of niche overlap suggests that the current differential distribution of mites of the two clades of T. evansi can be partially attributed to environmental niche differentiation. Overall this study shows how consensus strategies and analysis of niche overlap can be used jointly to draw conclusions on invasive threat considering different sources of uncertainty in species distribution modelling.

  8. Biología de oligonychus yothersi (mcgregor) (acari: tetranychidae) sobre aguacate persea americana mill. cv. lorena (lauraceae)

    Reyes, Julian C; Mesa, Nora; KONDO, T.

    2012-01-01

    Se estudió la biología del ácaro rojo Oligonychus yothersi en condiciones controladas(26 ± 3°C 56 ± 3% HR), sobre hojas maduras de aguacate, Persea americana Mill. cv.Lorena. Las observaciones se realizaron durante una generación del ácaro. La duraciónde todos los estados, a excepción del adulto, fueron los siguientes: huevo 119.06 horas±13.77 (4.96 días); larva 54.09 horas ± 18.48 (2.25 días), protocrisálida 18.22 ± 9.53(0.76 días), protoninfa 50.62 horas ± 13.29 (2.11 días), deutocrisalida ...

  9. Umbrales económicos para la araña roja Tetranychus urticae (acari: Tetranychidae) en mandarino

    Aguilar Fenollosa, Ernestina; Pascual Ruiz, Sara; Ibáñez Gual, María Victoria; Hurtado Ruiz, Mónica; Martínez Ferrer, M. T.

    2014-01-01

    Tetranychus urticae es una plaga clave en cítricos, especialmente en mandarina clementina. Se evaluó el daño de este ácaro sobre la producción de 24 árboles de clementino durante tres años consecutivos. Los árboles fueron visitados semanalmente, y se estimó tanto la población de araña roja como la de ácaros fitoseidos, así como la brotación. Al final de cada temporada, se recolectaron las clementinas, se pesaron, y se evaluó el daño ocasionado por T. urticae en fruto. Se encontró una relación...

  10. Change in abundance of three phytophagous mite species (Acari: Eriophyidae, Tetranychidae) on quackgrass in the presence of choke disease.

    Rector, Brian G; Czarnoleski, Marcin; Skoracka, Anna; Lembicz, Marlena

    2016-09-01

    Phytophagous mites and endophytic fungi may interact when sharing a host plant, potentially influencing one another's growth or population dynamics; however, interactions between them are poorly known and remain largely unexplored. In this study, quantitative associations between three species of phytophagous mites and the endophytic fungus Epichloë bromicola Leuchtm. & Schardl (Clavicipitaceae, Ascomycotina) on quackgrass, Elymus repens (L.) Gould are reported. The mites' abundance was assessed on field-collected grass shoots that were either exhibiting choke disease symptoms or without the fungus. Overall, the abundance of Tetranychus urticae and Aculodes mckenziei was significantly lower on quackgrass plants infected by E. bromicola compared to plants without the fungus. Conversely, populations of Abacarus hystrix were significantly larger on plants colonised by the fungus than on uninfected plants. Thus, the presence of this endophytic fungus may have divergent effects on different phytophagous mite species although the basis of these effects is not yet known. PMID:27388448

  11. Comparison and Field Validation of Binomial Sampling Plans for Oligonychus perseae (Acari: Tetranychidae) on Hass Avocado in Southern California.

    Lara, Jesus R; Hoddle, Mark S

    2015-08-01

    Oligonychus perseae Tuttle, Baker, & Abatiello is a foliar pest of 'Hass' avocados [Persea americana Miller (Lauraceae)]. The recommended action threshold is 50-100 motile mites per leaf, but this count range and other ecological factors associated with O. perseae infestations limit the application of enumerative sampling plans in the field. Consequently, a comprehensive modeling approach was implemented to compare the practical application of various binomial sampling models for decision-making of O. perseae in California. An initial set of sequential binomial sampling models were developed using three mean-proportion modeling techniques (i.e., Taylor's power law, maximum likelihood, and an empirical model) in combination with two-leaf infestation tally thresholds of either one or two mites. Model performance was evaluated using a robust mite count database consisting of >20,000 Hass avocado leaves infested with varying densities of O. perseae and collected from multiple locations. Operating characteristic and average sample number results for sequential binomial models were used as the basis to develop and validate a standardized fixed-size binomial sampling model with guidelines on sample tree and leaf selection within blocks of avocado trees. This final validated model requires a leaf sampling cost of 30 leaves and takes into account the spatial dynamics of O. perseae to make reliable mite density classifications for a 50-mite action threshold. Recommendations for implementing this fixed-size binomial sampling plan to assess densities of O. perseae in commercial California avocado orchards are discussed. PMID:26470355

  12. 60Co-γ Irradiation Affects the Enzymatic Antioxidant System of the Citrus Red Mite Panonychus citri (Acari: Tetranychidae

    Ke Zhang

    2014-05-01

    Full Text Available Radio-(60Co, which emits γ rays, has been used worldwide in pest control. The aim of the present study was to analyze the effect of effective-low-power 60Co-γ irradiation on the enzymatic antioxidant system of the citrus red mite Panonychus citri. One day old female adults were exposed to 0.4 kGy 60Co-γ irradiation and on the, 6th h, 1st day, 2nd day, and 5th day post treatment, the mites were euthanized for biochemical analysis. The activity of superoxide dismutase (SOD, catalase (CAT, peroxidase (POD, phenoloxidase (PO and acetylocholinesterase (AchE were investigated. POD and CAT activities, as well as SOD were higher in the irradiated mites. We found that exposure to 60Co-γ radiation resulted in increased activities of SOD, CAT, POD and decreased AchE activity. When the recovery time lasted till the 5th day, the activities of POD and PO were significantly lower than the control, whereas the SOD, CAT and AchE activities returned to control levels. Cells possess protein repair pathways to rescue oxidized proteins and restore their functions, but if these repair processes fail, oxidized proteins may become cytotoxic. Our results confirm the hypothesis that low dosages of 60Co-γ irradiation increase the level of oxidative stress in P. citri adults in a short time, causing meanwhile damage and sterility. The results of this study also indicate that stress caused by exposure to irradiation could inhibit the cholinergic system in P. citri.

  13. Seletividade de alguns agrotóxicos em uso na citricultura ao ácaro predador Neoseiulus californicus (McGregor) (Acari: Phytoseiidae) Residual toxicity of the main pesticides recommended in citrus orchards on predaceous mite Neoseiulus californicus (Mcgregor) (Acari: Phytoseiidae)

    Marcos Zatti da Silva; Carlos Amadeu Leite de Oliveira

    2006-01-01

    Objetivou-se avaliar o efeito dos principais agrotóxicos utilizados em citros sobre Neoseiulus californicus (McGregor), em condições de laboratório, por tratar-se de um fitoseídeo com grande potencialidade para ser utilizado no controle dos ácaros fitófagos na cultura. O método de bioensaio adotado foi o de contato direto e residual. Folhas de citros da variedade Pêra, contendo 25 fêmeas adultas de N. californicus procedentes de uma criação-estoque, foram pulverizadas em torre de Potter. Aval...

  14. Seletividade de acaricidas e inseticidas a ácaros predadores (Acari: Phytoseiidae encontrados em seringueira no centro-oeste do Brasil Side-effect of acaricides and insecticides to predatory mites (Acari: Phytoseiidae found in rubber-trees in mid-west Brazil

    Noeli Juarez Ferla

    2006-04-01

    Full Text Available Euseius concordis (Chant e Neoseiulus anonymus (Chant & Baker são ácaros predadores da família Phytoseiidae comumente encontrados em seringueira (Hevea brasiliensis Muell. Arg. em Mato Grosso, região centro-oeste do Brasil. Este trabalho foi realizado para verificar o efeito de acaricidas e inseticidas-acaricidas empregados em plantações de seringueira, e outros que teriam potencial para serem empregados contra insetos e ácaros considerados pragas dessa cultura, sobre E. concordis e N. anonymus. Utilizou-se o método residual de pulverização em superfície, recomendado como padrão pelo Grupo de Trabalho "Pesticidas e Artrópodes Benéficos", da Organização Internacional de Controle Biológico e Integrado de Plantas e Animais Nocivos/Seção Regional do Paleártico Oeste. Duas concentrações de cada um dos seguintes ingredientes ativos foram utilizadas: acefato, dicofol, endosulfan, formetanate, metomil, monocrotofós, óxido de fenbutatin e propargite. Uma das concentrações utilizadas foi uma média daquelas sugeridas pelos fabricantes para o controle de ácaros e insetos fitófagos presentes em outras culturas, uma vez que nenhum dos produtos testados tem registro para o uso em seringueira e a outra correspondeu à cerca de um terço da primeira. Endosulfan a 320ppm, dicofol a 100ppm e óxido de fenbutatin a 100 e 320ppm foram inócuos a E. concordis, enquanto que endosulfan a 320ppm e dicofol a 100ppm foram inócuos a N. anonymus. Acefato, formetanate e monocrotofós, nas concentrações testadas, foram nocivos às duas espécies.Euseius concordis (Chant and Neoseiulus anonymus (Chant & Baker are predatory mites of the family Phytoseiidae commonly found on rubber trees (Hevea brasiliensis Muell. Arg. in the State of Mato Grosso, in the mid-west Brazil. This work was conducted to verify the effect of acaricides and insecticides-acaricides occasionally used in rubber tree plantations, and other products that could be used against

  15. Plantas de ocorrência espontânea como substratos alternativos para fitoseídeos (Acari, Phytoseiidae em cultivos de seringueira Hevea brasiliensis Muell. Arg. (Euphorbiaceae Weeds as alternative substrates to phytoseiids (Acari, Phytoseiidae in rubber tree Hevea brasilienis, Muell. Arg. (Euphorbiaceae cultivation

    Marcos Roberto Bellini

    2005-03-01

    Full Text Available O objetivo deste estudo foi determinar as espécies de plantas espontâneas em áreas de cultivo de seringueira que poderiam servir com reservatório de ácaros predadores. O trabalho foi conduzido em Olímpia, Estado de São Paulo, Brasil, em dois cultivos de seringueira. A cada três meses, cerca de 1.000 cm³ de folhas de cada uma das cinco espécies de plantas espontâneas dominantes em cada cultivo foi tomada para determinar os fitoseídeos. Como as plantas espontâneas dominantes variaram durante o estudo, um total de 20 espécies foi verificado. Somente Cecropia sp. foi dominante em todo estudo, em ambos os cultivos. Um total de 336 fitoseídeos pertencentes às seguintes espécies foi encontrado: Euseius citrifolius Denmark & Muma, 1970, E. concordis (Chant, 1959, Galendromus annectens (DeLeon, 1958, Iphiseiodes zuluagai Denmark & Muma, 1972, Neoseiulus anonymus (Chant & Baker, 1965 e N. tunus (DeLeon, 1967. E. citrifolius (189 espécimes e N. tunus (138 espécimes foram os mais abundantes. A maior abundância (231espécimes e diversidade (5 espécies foram observadas sobre Cecropia sp. Sobre esta planta foi encontrado o maior número de ácaros por amostra (29 espécimes, seguida por Piper duncum Linnaeus (22, Guarea sp. (18 e Ageratum conyzoides Linnaeus (12. E. citrifolius tem sido determinado como um dos ácaros predadores mais abundantes sobre seringueiras cultivadas na região onde este trabalho foi conduzido. Cecropia sp. parece ser uma das plantas espontâneas mais importantes consideradas como reservatório de E. citrifolius porque está continuamente presente nos cultivos. Estudos complementares poderiam indicar a viabilidade de se manejar esta planta nas áreas de cultivo de seringueira para permitir o controle biológico dos ácaros-praga desta cultura.The objective of this study was to determine weed species of rubber tree cultivation areas that could serve as reservoirs of predatory mites. The work was conducted in Ol

  16. Atividade do acaricida etoxazol sobre a mortalidade e reprodução do ácaro-da-leprose Brevipalpus phoenicis (Geijskes (Acari: Tenuipalpidae, em citros Activity of the etoxazole acaricide on the mortality and reproduction of the citrus leprosis mite Brevipalpus Phoenicis (Geijskes (Acari: Tenuipalpidae, in citrus

    Fernando Juari Celoto

    2010-12-01

    Full Text Available O objetivo deste trabalho foi avaliar a atividade do acaricida etoxazol, no controle e reprodução do ácaro B. phoenicis. Para tanto, foram demarcadas com cola adesiva arenas de cinco centímetros de diâmetro em frutos de citros com alta infestação do ácaro. O ensaio foi delineado em parcelas inteiramente casualizadas, com oito tratamentos e quatro repetições. Em cada arena foram contados o número de ácaros adultos, jovens e ovos. Os tratamentos constaram dos seguintes acaricidas e doses em g i.a./100 L de água: etoxazol 110 SC (1,1; 1,65; 2,75 e 5,5; hexitiazoxi 500 PM (0,75; flufenoxuron 100 CE (3; cihexatina 500 PM (25, aplicados diretamente sobre as arenas. Os frutos foram mantidos em câmara de germinação tipo BOD. com temperatura de 25 ± 2 ºC e fotofase de 12 horas. Diariamente, foram contados o número de ácaros adultos, jovens e ovos, com auxílio de microscópio esteroscópio. Os parâmetros avaliados foram a atividade ovicida, esterilização de fêmeas e efeito sobre formas jovens. Constatou-se que o etoxazol provocou mortalidade de formas jovens do ácaro-da-leprose superior a 95%, nas doses a partir de 1,1 g i.a. /100 L de água. Ovos tratados com etoxazol, nas doses a partir de 1,65 g i.a. /100 L de água, apresentaram inviabilidade média de 60%. O etoxazol apresentou efeito esterilizante sobre fêmeas nas doses a partir de 2,75 g i.a./100 L de água, inviabilizando 95% dos ovos.The objective of this work was to evaluate the activity of the etoxazole acaricide, on the mortality and reproduction of the citrus leprosies mite, B. phoenicis. A five centimeter diameter arena were demarcated with adhesive glue, in citrus fruits with high infestation of the mite. The design was entirely randomized plots with eight treatments and four replications. In each arena were counted the number of adults, young and eggs of B. phoenicis. The treatments consisted the following acaricides and doses in g a.i./100 L of water: etoxazole

  17. Carrapatos do gênero amblyomma (acari: ixodidae e suas relações com os hospedeiros em área endêmica para febre maculosa no Estado de São Paulo Ticks of genus Amblyomma (Acari: Ixodidae and their relationship with hosts in endemic area for spotted fever in the state of São Paulo

    Carlos Alberto Perez

    2008-12-01

    Full Text Available Foram avaliadas 7 espécies da mastofauna e 36 da avifauna quanto à prevalência e intensidade de infestação por carrapatos na ESALQ/USP, no Município de Piracicaba, SP. Analisaram-se 52 indivíduos da mastofauna e 158 da avifauna, parasitados por 12418 carrapatos. Os exemplares adultos (N= 7343 foram encontrados em parasitismo nas capivaras enquanto que os imaturos foram, na maioria, coletados de pequenos mamíferos e aves. Os principais hospedeiros para as formas imaturas, em ordem decrescente, foram gambás (69,1%, capivaras (24,4% e urubus (3,7%. Entre a avifauna, o urubu apresentou o maior número de carrapatos com 69,9%, seguido por indivíduos das famílias Thamnophilidae e Turdidae. Os carrapatos adultos encontrados em capivaras foram A. cajennense (80,8% e A. dubitatum (19,2%. Ambas as espécies foram também coletadas em gambás, correspondendo a 72,4% e 27,6%, respectivamente. Pela facilidade de captura e atratividade de Amblyomma spp. o gambá pode ser usado como bioindicador de infestação em locais endêmicos para febre maculosa. Considerando os índices de parasitismo e prevalência bem como de abundância de carrapatos, susceptibilidade dos hospedeiros, proliferação e susceptibilidade para infecção por R. rickettsi, capivaras e gambás são potenciais hospedeiros amplificadores desse microrganismo no Campus da ESALQ, enquanto eqüídeos, urubus e gatos atuam como hospedeiros secundários.Seven species of mammals and 36 of birds were investigated to determine the tick prevalence and intensity of infestation. The study was conducted at the Esalq/USP in Piracicaba municipality, State of São Paulo. It was collected 52 mammals and 158 birds parasitized by 12,418 ticks. Adult ticks (N= 7,343 were found on capybaras, while the immature were mainly collected on small mammals and birds. The main hosts for immatures in descending order were opossums (69.1%, capybara (24.4% and black vultures (3.7%. Among the avifauna, the black

  18. New Oppioidea taxa from Madagascar (Acari: Oribatida

    Mahunka, S.

    2012-07-01

    Full Text Available Within the framework of the continuous survey of the Madagascan Oribatida Fauna some newly surveyed Oppioidea (Acari: Oribatida species are discussed. Altogether 15 species are listed of the recently studied, identified and described taxa originating from several sites of the island (Malagasy Republic. Seven species of them are new to science and some other known only from few localities. One species represents also a new genus, Interbelba gen. nov. Three species, Berniniella bicarinata (Paoli, 1908, Quadroppia circumita (Hammer, 1961 and Discosuctobelba variosetosa (Hammer, 1961are recorded from Madagascar for the first time. With 22 figures.

  19. INFLUÊNCIA DO PESO INICIAL E DA ESTAÇÃO DO ANO NA CONVERSÃO EM OVOS DE FÊMEAS DE Boophilus microplus (ACARI: IXODIDAE

    Abraão Garcia Gomes

    2006-10-01

    Full Text Available Este trabalho objetivou avaliar a influência do peso inicial da fêmea e da estação do ano na conversão em ovos de Boophilus microplus. O índice de eficiência reprodutiva (IER de 411 fêmeas pesando entre 31 e 360 mg foi correlacionado com seu peso inicial. Para avaliar a influência das diferentes estações do ano neste parâmetro biológico, dez fêmeas ingurgitadas de B. microplus pesando acima de 150 mg foram colhidas, a cada duas semanas, e mantidas em condições climáticas controladas até a determinação do IER. Uma relação curvilinear foi observada entre o peso das fêmeas e o IER. Fêmeas pesando entre 151 e 360 mg tiveram IER’s mais altos (variando de 44,9% a 51,4% do que aquelas mais leves, as quais apresentaram uma menor capacidade de conversão em ovos (variando de 31,4% a 39,0%. Foi observado que a capacidade de as fêmeas converterem o peso em ovos variou sazonalmente. Os mais altos IER foram observados no período seco, de abril a setembro (47,6% a 58,3%, do que no período chuvoso, de outubro a março (40,2% a 43,6% (P<0,05. PALAVRAS-CHAVE: Boophilus microplus, biologia, conversão em ovos, bovinos.

  20. Ocorrência de Amblyomma fuscum Neumann, 1899 e Amblyomma humerale Koch, 1844 (Acari: Ixodidae em Bufo arenalis no estado de São Paulo, Brasil Occurence of Amblyomma fuscum Neumann, 1899 and Amblyomma humerale Koch, 1844 (Acari: Ixodidae in Bufo arenalis in the state of São Paulo, Brazil

    Afonso Lodovico Sinkoc

    1997-06-01

    Full Text Available O objetivo deste trabalho é relatar a ocorrência do parasitismo monoespecífico de A. fuscum NEUMANN, 1899 e A. humerale KOCH, 1844 em sapos (Bufo arenalis no Município de Rosana, Estado de São Paulo, Brasil. Este relato caracteriza um novo hospedeiro e uma nova localização geográfica para estas duas espécies de carrapatos.The objective of this work is to describe the occurence of the monoespecific parasitism of A. fuscum NEUMANN, 1899 and A. humerale KOCH, 1844 in toads (Bufo arenalis from the County of Rosana, State of São Paulo, Brazil. This is the description of a new host and new geographic site for those two species.

  1. Seletividade de alguns agrotóxicos em uso na citricultura ao ácaro predador Neoseiulus californicus (McGregor (Acari: Phytoseiidae Residual toxicity of the main pesticides recommended in citrus orchards on predaceous mite Neoseiulus californicus (Mcgregor (Acari: Phytoseiidae

    Marcos Zatti da Silva

    2006-08-01

    Full Text Available Objetivou-se avaliar o efeito dos principais agrotóxicos utilizados em citros sobre Neoseiulus californicus (McGregor, em condições de laboratório, por tratar-se de um fitoseídeo com grande potencialidade para ser utilizado no controle dos ácaros fitófagos na cultura. O método de bioensaio adotado foi o de contato direto e residual. Folhas de citros da variedade Pêra, contendo 25 fêmeas adultas de N. californicus procedentes de uma criação-estoque, foram pulverizadas em torre de Potter. Avaliaram-se a mortalidade do ácaro 72 horas após a aplicação e o efeito dos produtos na viabilidade dos ovos. Quanto à seletividade, conforme proposta da "Organização Internacional para o Controle Biológico e Integrado de Plantas e Animais Nocivos" (IOBC, os produtos foram classificados como: classe 1 inócuo (E99%, clorfenapyr, dicofol e pyridaben. N. californicus mostrou-se tolerante a diversos agrotóxicos, o que viabiliza sua utilização, juntamente com produtos químicos pertencentes a grupamentos distintos e de diferentes mecanismos de ação, o que atende a um manejo adequado de controle dos principais ácaros fitófagos da cultura dos citros.This work was carried out to determine the residual toxicity of the main pesticides used in citrus, on Neoseiulus californicus (McGregor under laboratory conditions. For this, it was used the residual contact bioassay. Citrus leaves of the variety "Pêra" were sprayed in a Potter tower. The residual action was evaluated at 2 hours and 1; 3; 5; 7; 10; 14 e 21 days after treatment. Ten adult females of N. californicus were transferred to each ring, together with an enough amount of Tetranychus urticae nymphs and adults to feed the predator. Mortality evaluations were performed at 72 hours after treatment. Bioassays indicate that the pesticides: acrinathrin, deltamethrin, dinocap, sulphur, fenpropathrin, fenbutatin oxide and propargite were innocuous to N. californicus. On the other hand, abamectin

  2. PREVALÊNCIA DE ENDOSSIMBIONTES Cardinium EM POPULAÇÕES DE ÁCAROS Brevipalpus phoenicis DE DIFERENTES REGIÕES BRASILEIRAS.

    Em B. phoenicis (Geijskes) (Acari: Tenuipalpidae), ácaro vetor de importantes fitovírus, a presença de endossimbiontes do gênero Cardinium relaciona-se ao processo de feminilização dos machos, acarretando em elevada proporção de fêmeas em populações naturais. Até o momento, não é conhecido se este e...

  3. Two new Uropodina species from Ethiopia (Acari: Mesostigmata)

    Kontschán, J.; Starý, Josef

    2013-01-01

    Roč. 54, č. 1 (2013), s. 49-56. ISSN 1681-5556 Institutional research plan: CEZ:AV0Z60660521 Institutional support: RVO:60077344 Keywords : Acari * Uropodina * Afrotropical * Ethiopia * new species * new synonymy Subject RIV: EG - Zoology Impact factor: 0.500, year: 2013

  4. New Uropodina species and records from Malaysia (Acari: Mesostigmata)

    Kontschán, J.; Starý, Josef

    2012-01-01

    Roč. 58, č. 2 (2012), s. 177-192. ISSN 1217-8837 Institutional research plan: CEZ:AV0Z60660521 Keywords : Acari * new records * new species * Malaysia Subject RIV: EH - Ecology, Behaviour Impact factor: 0.472, year: 2012

  5. Effect Of Agrotechnical Measures And Varieties On Seasonal Dynamics Of Tetranychus Urticae Koch (Acari, Trombidiformes, Tetranychidae On High Tunnel-Cultivated Garden Strawberries

    Salmane Ineta

    2015-04-01

    Full Text Available The aim of the present study was to determine the seasonal pattern of two-spotted spider mite Tetranychus urticae on strawberries cultivated in polythene-covered high tunnels in temperate climatic conditions. Various cultivars were used and the effect of modification of plant covering indices on abundance and incidence of these mites was also tested. The number of two-spotted spider mites was relatively low at the beginning of the vegetation season and started to increase when average air temperature rose above 20 to 25 °C. In the experiment two types of tunnels differing in additional plant cover were used. The maximum mite abundance did not significantly vary between varieties in tunnel 1 conditions, but it was significantly lower for variety 'Sonata' in tunnel 2 conditions. Mite numbers significantly declined after strawberry foliage mowing and removal of polythene cover. Mite development was prolonged in tunnel 1, where additional cover of plants was used and higher early season air temperature was recorded in comparison to tunnel 2. It was concluded that increase in early season temperature can increase two-spotted spider mite abundance and have a more negative effect on strawberry plants in respect of foliage damage by mites.

  6. Foraging on and consumption of two species of papaya pest mites, Tetranychus kanzawai and Panonychus citri (Acari: Tetranychidae), by Mallada basalis (Neuroptera: Chrysopidae).

    Cheng, Ling Lan; Nechols, James R; Margolies, David C; Campbell, James F; Yang, Ping Shih; Chen, Chien Chung; Lu, Chiu Tung

    2009-06-01

    Tetranychus kanzawai Kishida and Panonychus citri (McGregor) are two major acarine pests of the principal papaya variety in Taiwan, and they often co-occur in the same papaya screenhouses. This study measured prey acceptability, foraging schedule, short-term consumption rate, and handling time of larvae of a domesticated line of the green lacewing, Mallada basalis (Walker), in no-choice tests with different life stages of these two mite pests. After a period of prey deprivation, all three larval instars of M. basalis exhibited a high rate of acceptance of all life stages of both T. kanzawai and P. citri. In 2-h trials, second- and third-instar predators foraged actively most of the time, whereas first instars spent approximately 40% of the time at rest. Consumption increased and prey handling time decreased as predator life stage advanced and prey stage decreased. Third-instar lacewings consumed an average of 311.4 T. kanzawai eggs (handling time: 6.7 s/egg) and 68.2 adults (handling time: 58.8 s/adult), whereas first instars consumed 19.6 eggs (handling time: 23.6 s/egg) and 4.0 adults (handling time: 633.4 s/adult). M. basalis generally consumed more P. citri than T. kanzawai. Except for prey eggs, handling times of T. kanzawai were generally longer than those of P. citri by all M. basalis instars. Handling times were shorter, and consumption were greater, at the higher P. citri density than at the lower one, whereas there were generally no significant differences in prey acceptability and foraging time between those two densities. This study suggests that M. basalis larvae may have high potential for augmentative biological control of mites on papayas. PMID:19508780

  7. Exposure to Diflubenzuron Results in an Up-Regulation of a Chitin Synthase 1 Gene in Citrus Red Mite, Panonychus citri (Acari: Tetranychidae)

    Wen-Kai Xia; Tian-Bo Ding; Jin-Zhi Niu; Chong-Yu Liao; Rui Zhong; Wen-Jia Yang; Bin Liu; Wei Dou; Jin-Jun Wang

    2014-01-01

    Chitin synthase synthesizes chitin, which is critical for the arthropod exoskeleton. In this study, we cloned the cDNA sequences of a chitin synthase 1 gene, PcCHS1, in the citrus red mite, Panonychus citri (McGregor), which is one of the most economically important pests of citrus worldwide. The full-length cDNA of PcCHS1 contains an open reading frame of 4605 bp of nucleotides, which encodes a protein of 1535 amino acid residues with a predicted molecular mass of 175.0 kDa. A phylogenetic ...

  8. Evaluation of suitable reference genes for quantitative RT-PCR during development and abiotic stress in Panonychus citri (McGregor) (Acari: Tetranychidae).

    Niu, Jin-Zhi; Dou, Wei; Ding, Tian-Bo; Yang, Li-Hong; Shen, Guang-Mao; Wang, Jin-Jun

    2012-05-01

    Quantitative real time reverse transcriptase polymerase chain reaction (RT-qPCR) is preferred for gene expression analysis in living organisms. Currently, it is a valuable tool for biological and ecological studies as it provides a relatively straightforward way to assess the relevance of transcriptional regulation under developmental and stress tolerance conditions. However, studies have shown that some commonly used reference genes varied among different experimental treatments, thus, systematic evaluation of reference genes is critical for gene expression profiling, which is often neglected in gene expression studies of arthropods. The aim of this study is to identify the suitable reference genes for RT-qPCR experiments involving various developmental stages and/or under abiotic stresses in citrus red mite Panonychus citri, a key pest in citrus orchards worldwide. GeNorm, NormFinder, and Bestkeeper software analysis indicates that elongation factor-1 alpha (ELF1A), RNA polymerase II largest subunit, alpha tublin, and glyceraldhyde-3-phosphate dehydrogenase (GAPDH) are the most stable reference genes in various developmental stages, meanwhile, ELF1A and GAPDH were the most stable reference genes under various abiotic stresses. Furthermore, this study will serve as a resource to screen reference genes for gene expression studies in any other spider mite species. PMID:22203483

  9. Molecular cloning and characterization of a microsatellite locus found in an RAPD marker of a spider mite, Panonychus citri (Acari: Tetranychidae).

    Osakabe, M; Hinomoto, N; Toda, S; Komazaki, S; Goka, K

    2000-01-01

    Genetic markers were searched using PCR with 40 kinds of decanucleotide primers to investigate DNA polymorphism in Panonychus citri. A region consisting of a variable number of CT tandem repeats (microsatellite) was found in a fragment amplified with the OPB10 primer. The microsatellite differed in size by ca. 100bp among several P. citri populations screened and was derived from at least seven alleles. This region was characteristic of P. mori and P. osmanthi, but was lacking in P. ulmi. The flanking regions were highly conserved among these species. PMID:11156164

  10. Exposure to Diflubenzuron Results in an Up-Regulation of a Chitin Synthase 1 Gene in Citrus Red Mite, Panonychus citri (Acari: Tetranychidae

    Wen-Kai Xia

    2014-02-01

    Full Text Available Chitin synthase synthesizes chitin, which is critical for the arthropod exoskeleton. In this study, we cloned the cDNA sequences of a chitin synthase 1 gene, PcCHS1, in the citrus red mite, Panonychus citri (McGregor, which is one of the most economically important pests of citrus worldwide. The full-length cDNA of PcCHS1 contains an open reading frame of 4605 bp of nucleotides, which encodes a protein of 1535 amino acid residues with a predicted molecular mass of 175.0 kDa. A phylogenetic analysis showed that PcCHS1 was most closely related to CHS1 from Tetranychus urticae. During P. citri development, PcCHS1 was constantly expressed in all stages but highly expressed in the egg stage (114.8-fold higher than in the adult. When larvae were exposed to diflubenzuron (DFB for 6 h, the mite had a significantly high mortality rate, and the mRNA expression levels of PcCHS1 were significantly enhanced. These results indicate a promising use of DFB to control P. citri, by possibly acting as an inhibitor in chitin synthesis as indicated by the up-regulation of PcCHS1 after exposure to DFB.

  11. Exposure to diflubenzuron results in an up-regulation of a chitin synthase 1 gene in citrus red mite, Panonychus citri (Acari: Tetranychidae).

    Xia, Wen-Kai; Ding, Tian-Bo; Niu, Jin-Zhi; Liao, Chong-Yu; Zhong, Rui; Yang, Wen-Jia; Liu, Bin; Dou, Wei; Wang, Jin-Jun

    2014-01-01

    Chitin synthase synthesizes chitin, which is critical for the arthropod exoskeleton. In this study, we cloned the cDNA sequences of a chitin synthase 1 gene, PcCHS1, in the citrus red mite, Panonychus citri (McGregor), which is one of the most economically important pests of citrus worldwide. The full-length cDNA of PcCHS1 contains an open reading frame of 4605 bp of nucleotides, which encodes a protein of 1535 amino acid residues with a predicted molecular mass of 175.0 kDa. A phylogenetic analysis showed that PcCHS1 was most closely related to CHS1 from Tetranychus urticae. During P. citri development, PcCHS1 was constantly expressed in all stages but highly expressed in the egg stage (114.8-fold higher than in the adult). When larvae were exposed to diflubenzuron (DFB) for 6 h, the mite had a significantly high mortality rate, and the mRNA expression levels of PcCHS1 were significantly enhanced. These results indicate a promising use of DFB to control P. citri, by possibly acting as an inhibitor in chitin synthesis as indicated by the up-regulation of PcCHS1 after exposure to DFB. PMID:24590130

  12. Chemical Composition and Acaricidal Effects of Essential Oils of Foeniculum vulgare Mill. (Apiales: Apiaceae and Lavandula angustifolia Miller (Lamiales: Lamiaceae against Tetranychus urticae Koch (Acari: Tetranychidae

    Asgar Ebadollahi

    2014-01-01

    Full Text Available Utilization of synthetic acaricides causes negative side-effects on nontarget organisms and environment and most of the mite species such as two spotted spider mite, Tetranychus urticae Koch, are becoming resistant to these chemicals. In the present study, essential oils of fennel, Foeniculum vulgare Mill., and lavender, Lavandula angustifolia Miller, were hydrodistilled using Clevenger apparatus and chemical composition of these oils was analyzed by GC-MS. Anethole (46.73%, limonene (13.65%, and α-fenchone (8.27% in the fennel essential oil and linalool (28.63%, 1,8-cineole (18.65%, and 1-borneol (15.94% in the lavender essential oil were found as main components. Contact and fumigant toxicity of essential oils was assessed against adult females of T. urticae after 24 h exposure time. The essential oils revealed strong toxicity in both contact and fumigant bioassays and the activity dependeds on essential oil concentrations. Lethal concentration 50% for the population of mite (LC50 was found as 0.557% (0.445–0.716 and 0.792% (0.598–1.091 in the contact toxicity and 1.876 μL/L air (1.786–1.982 and 1.971 μL/L air (1.628–2.478 in the fumigant toxicity for fennel and lavender oils, respectively. Results indicated that F. vulgare and L. angustifolia essential oils might be useful for managing of two spotted spider mite, T. urticae.

  13. The effects of prey patchiness, predator aggregation, and mutual interference on the functional response of Phytoseiulus persimilis feeding on Tetranychus urticae (Acari: Phytoseiidae, Tetranychidae)

    Nachman, Gösta

    abundant prey. The effects of non-homogenous distributions and degree of spatial overlap between prey and predators on the per capita predation rate were studied by means of a stage-specific predation model that averages the predation rates over all the local populations inhabiting the individual leaves...... even distribution when its density is low and a more patchy distribution when density increases. Mutual interference between searching predators reduces the predation rate, but the effect is negligible. The stage-specific functional response model was compared with two simpler models without explicit......The spatial distributions of two-spotted spider mites Tetranychus urticae and their natural enemy, the phytoseiid predator Phytoseiulus persimilis, were studied on six full-grown cucumber plants. Both mite species were very patchily distributed and P. persimilis tended to aggregate on leaves with...

  14. Interactions in a tritrophic acarine predator-prey metapopulation system V: Within-plant dynamics of Phytoseiulus persimilis and Tetranychus urticae (Acari : Phytoseiidae, Tetranychidae)

    Nachman, G.; Zemek, Rostislav

    2003-01-01

    Roč. 29, - (2003), s. 35-68. ISSN 0168-8162 Institutional research plan: CEZ:AV0Z5007907 Keywords : Biological control * bottom-up factor * Phytoseiulus persimilis Subject RIV: EH - Ecology, Behaviour Impact factor: 0.497, year: 2003

  15. Manejo integrado de la plaga Tetranychus urticae (Acari: Tetranychidae) en cultivos de frutilla del Cinturón Hortícola Platense

    Gugole Ottaviano, María Fernanda

    2013-01-01

    En el marco de la sustentabilidad de los sistemas agrícolas, el Manejo Integrado de Plagas propone utilizar todas las técnicas y métodos disponibles y compatibles entre sí, para mantener a la población de una plaga en niveles por debajo de aquellos que causan daño económico. Se basa en el análisis de la relación costo/beneficio, considerando los intereses e impactos sobre los productores, la sociedad y el ambiente. Este sistema de manejo tiene un enfoque ecológico y multidisciplinario que req...

  16. Chemical Composition and Acaricidal Effects of Essential Oils of Foeniculum vulgare Mill. (Apiales: Apiaceae) and Lavandula angustifolia Miller (Lamiales: Lamiaceae) against Tetranychus urticae Koch (Acari: Tetranychidae)

    Asgar Ebadollahi; Jalal Jalali Sendi; Alireza Aliakbar; Jabraeil Razmjou

    2014-01-01

    Utilization of synthetic acaricides causes negative side-effects on nontarget organisms and environment and most of the mite species such as two spotted spider mite, Tetranychus urticae Koch, are becoming resistant to these chemicals. In the present study, essential oils of fennel, Foeniculum vulgare Mill., and lavender, Lavandula angustifolia Miller, were hydrodistilled using Clevenger apparatus and chemical composition of these oils was analyzed by GC-MS. Anethole (46.73%), limonene (13.65%...

  17. Effects of host plants on distribution, abundance, developmental time and life table parameters of Oligonychus afrasiaticus (McGregor (Acari: Tetranychidae

    Sameh Ben Chaaban

    2012-01-01

    Full Text Available The biology and ecology of the date palm mite O. afrasiaticus have been studied through regular inspection in Tunisian oases and laboratory observations. Results indicate that the start date of fruit infestation varied between years and by date palm variety. Start dates ranged from the first week to the third week of July. The period spent by the mite on fruits varied from one variety to another; lasting 8 weeks on the Deglet Noor variety, 2 to 5 weeks on Alig, 2 to 4 weeks on Kentichi dates, and 2 to 4 weeks on Bessr fruits. The Deglet Noor variety was the most susceptible to O. afrasiaticus. Mite populations on the pinnae remained low from May through December. During autumn and spring, O. afrasiaticus was found on sorghum leaves in the orchard ground-cover. A life table study in the laboratory at 27°C on six host plants (fruits of date palms varieties Deglet Noor, Alig, Kentichi, Bessr, and Deglet Noor pinnae and sorghum leaves showed that the life cycle of O. afrasiaticus differed among host plants with average values ranging between 13 on Alig fruits and 10.9 days on sorghum leaves. Relatively high fecundity was found on sorghum leaves (2 eggs/female/day during 5.2 oviposition days, while low fecundity values occurred on Deglet Noor pinnae and Alig fruits with 0.7 eggs/female/day during 5.4 days. Average longevity of O. afrasiaticus females ranged from 13.4 to 7.5 days on Deglet Noor fruits and sorghum leaves, respectively. Intrinsic rate of increase (r m was highest on sorghum leaves (0.171 and Deglet Noor fruits (0.166, and lowest on Alig fruits (0.103. Greater knowledge of life history traits and seasonal abundance of this species is needed in order to design appropriate control strategies.

  18. A novel action of highly specific acaricide; bifenazate as a synergist for a GABA-gated chloride channel of Tetranychus urticae [Acari: Tetranychidae].

    Hiragaki, Susumu; Kobayashi, Takeru; Ochiai, Noriaki; Toshima, Kayoko; Dekeyser, Mark A; Matsuda, Kazuhiko; Takeda, Makio

    2012-06-01

    Bifenazate is a very selective acaricide that controls the spider mite, Tetranychus urticae. Bifenazate is the first example of a carbazate acaricide. Its mode of action remains unclear. Bifenazate and its active metabolite diazene induce paralysis in spider mites, suggesting that they may act on the nervous system. Here we have employed a homologue (TuGABAR) of RDL (Resistance to dieldrin), a subunit of ionotropic γ-aminobutyric acid (GABA) receptor, from T. urticae to investigate the action of bifenazate and its active metabolite diazene on this receptor function. Although neither acaricide showed a GABA agonist action, 30 μM of bifenazate or diazene significantly enhanced the GABA-induced response of TuGABAR in a dose-dependent manner, shifting the EC(50) of GABA from 24.8 μM to 4.83 μM and 10.8 μM, respectively. This action demonstrates a positive allosteric modulator effect of bifenazate on T. urticae GABA receptors. This synergistic action is likely the result of bifenazate binding to a site distinct from that of the GABA binding site causing a conformational change that affects the magnitude of the GABA response. Precisely how the observed GABA synergist action correlates with the acaricidal activity of bifenazate, if at all, has yet to be determined. PMID:22330756

  19. Effect of gamma radiation on the toxicity of milbemectin and chlorfenapyr in acaricide resistant and susceptible strains of Tetranychus urticae Koch (Acari: Tetranychidae)

    Nicastro, Roberto L.; Arthur, Valter; Machi, Andre R., E-mail: rnicastro@cena.usp.br, E-mail: arthur@cena.usp.br [Laboratorio de Radiobiologia e Ambiente (CENA/USP), Piracicaba, SP (Brazil); Sato, Mario E., E-mail: mesato@biologico.sp.gov.br [Laboratorio de Acarologia, Instituto Biologico, Campinas, SP (Brazil)

    2011-07-01

    The spider mite Tetranychus urticae Koch is considered one of the most important phytophagous mites, causing considerable damage in several agricultural crops. The aim of this study was to evaluate the effect of gamma radiation on the toxicity of the acaricides milbemectin and chlorfenapyr in resistant and susceptible strains of T. urticae. The R and S strains for milbemectin and chlorfenapyr were irradiated with gamma radiation at Gamma cell-220 source at doses of 5, 10, 20, 40 e 80 Gy. Five concentrations of milbemectin and chlorfenapyr were evaluated, making applications 24 hours after irradiation. Mites of the controls were sprayed with the same acaricide concentrations used for the R and S strains but they were not exposed to gamma radiation. Experiments on the effects of gamma radiation on the growth rates of mites for acaricide resistant and susceptible strains of T. urticae were also carried out. Tests with the Milbemectin S strain showed an increased susceptibility to the acaricide milbemectin, when the mites were irradiated (20 Gy), in comparison with the control (non irradiated mites). For the Milbemectin R strain, there was no significant influence of gamma irradiation on the toxicity of milbemectin to the mites of this strain. For the Chlorfenapyr S strain, the effect of gamma radiation was similar to that observed for Milbemectin S strain, with increased toxicity of chlorfenapyr to the mites of this susceptible strain. In the case of the Chlorfenapyr R strain, the mites exposed to gamma radiation showed to be more tolerant to chlorfenapyr, considering the LC{sub 10} values. The same trend was observed for the LC{sub 50} values, however, there was no significant difference with the control. The experiments showed that doses of 200 and 300 Gy eliminated the mite populations of acaricide resistant and susceptible strains of T. urticae, in a period of ten days. The dose of 100 Gy did not lead to total elimination of the mite populations, but reduced significantly the egg viability of T. urticae. The highest effect was observed for the Chlorfenapyr S strain, for which the instantaneous growth rate (ri)of irradiated mites was 3.8 times lower than that of the control. The effect of gamma radiation was lower for the Chlorfenapyr R strain, with the ri value 40% higher than that of the Chlorfenapyr S strain. (author)

  20. Dinámica del sistema depredador-presa de las arañas rojas y los fitoseidos (Acari: Tetranychidae, Phytoseiidae) en cultivos hortícolas

    GÓMEZ MOYA, CRISTINA ANTONIA

    2012-01-01

    Las arañas rojas constituyen una de las plagas más importantes en la producción de hortalizas del litoral mediterráneo español, tanto en invernadero como al aire libre. Estos ácaros son controlados por medios químicos, lo cual representa costos significativos en términos económicos y ecológicos. Otra alternativa sería el control biológico usando fitoseidos que son depredadores naturales de los tetraníquidos. Sin embargo, se desconocen informaciones básicas sobre la dinámica y la óptima relaci...

  1. Development and reproduction of Panonychus citri (Prostigmata: Tetranychidae) on different species and varieties of citrus plants.

    Zanardi, Odimar Zanuzo; Bordini, Gabriela Pavan; Franco, Aline Aparecida; de Morais, Matheus Rovere; Yamamoto, Pedro Takao

    2015-12-01

    The species and varieties of citrus plants that are currently grown can favor the population growth of the citrus red mite Panonychus citri (McGregor) (Prostigmata: Tetranychidae) and alter the pest management programs in citrus groves. In this study we evaluated, in the laboratory, the development and reproduction of P. citri and estimated its life table parameters when reared on four varieties of Citrus sinensis (L.) Osbeck (Valencia, Pera, Natal, and Hamlin), one variety of Citrus reticulata Blanco (Ponkan) and one variety of Citrus limon (L.) Burm. (Sicilian). The incubation period and egg viability were not affected by the host plant. However, the development and survival of the immature stage were significantly lower on Hamlin orange than on Valencia, Pera and Natal oranges, Ponkan mandarin and Sicilian lemon. The fecundity and oviposition period of females were lower on Hamlin orange than on the other hosts. Mites reared on Valencia orange and Sicilian lemon had a higher net reproductive rate (R 0 ), intrinsic growth rate (r) and finite rate of increase (λ), and a shorter interval between generations (T) than on Pera, Natal and Hamlin oranges and Ponkan mandarin. On the other hand, mites reared on Hamlin orange had the lowest R 0 , r and λ and the highest T among the hosts. Based on the results obtained we recommend that for Valencia orange and Sicilian lemon, the mite monitoring programs should be more intense to detect the initial infestation of pest, avoiding the damage in plants and the increase in production costs. PMID:26459376

  2. Study of Acari and Collembola Populations in Four Cultivation Systems in Dourados - MS

    Rosilda Mara Mussury

    2002-09-01

    Full Text Available The impact four cultivation systems on the soil fauna was studied, using Oribatida and Gamasida acarids as bioindicators and collembolan. The research was carried out in experimental fields, located in EMBRAPA - CPAO in Dourados, Centerwest of Brazil from July 1997 to December 1999. The constant pasture system presented smaller impact on the soil fauna followed by agricultural cattle rotation and a direct plantation system. In the conventional plantation series, the populational density of the mesofauna organisms was low, especially collembolan families.O impacto de quatro sistemas de cultivo sobre a fauna de solo foram estudados, utilizando-se como bioindicadores os acari Oribatida e Gamasida e os Collembola. A pesquisa foi conduzida em campos experimentais, localizados na EMBRAPA - CPAO no município de Dourados, MS, no período de julho de 1997 à dezembro de 1999. O sistema de pastagem contínua apresentou menor impacto sobre a fauna de solo seguido da rotação agricultura pecuária e do sistema de plantio direto. Nas sucessões do plantio convencional, a densidade populacional dos organismos da mesofauna foi baixa, em especial as famílias de colembolos.

  3. Toxicidade de extratos de nim (Azadirachta indica) ao ácaro-vermelho-do-cafeeiro Oligonychus ilicis Toxicity of neem extracts (Azadirachta indica) to the coffee red mite Oligonychus ilicis

    Sheila Abreu Mourão; José Cola Zanuncio; Angelo Pallini Filho; Raul Narciso Carvalho Guedes; Adonai Bruneli de Camargos

    2004-01-01

    O objetivo deste trabalho foi estudar a toxicidade aguda e crônica de extratos de óleo de torta, de sementes e de folhas de Azadirachta indica A. Juss (nim) a fêmeas do ácaro-vermelho-do-cafeeiro Oligonychus ilicis (McGregor) (Acari: Tetranychidae). A mortalidade e a taxa instantânea de crescimento populacional de fêmeas adultas desse ácaro foram avaliadas em discos de folhas de cafeeiro com 3,5 cm de diâmetro, impregnados com resíduo seco dos extratos de nim e flutuando em água. As concentra...

  4. A new species of Brevipalpus Donnadieu (Acari: Tenuipalpidae) and key to the Egyptian species.

    Halawa, Alaa M; Fawzy, Magdy M

    2014-01-01

    A new species, Brevipalpus noranae sp. nov. (Acari: Tenuipalpidae) is described and illustrated from females collected on Malus domestica Borkh and Citrus aurantium L. A key to the species of the genus Brevipalpus present in Egypt is provided. PMID:24869810

  5. Three new .i.Trachyuropoda./i. (Acari: Uropodina: Trachyuropodidae) species from the Neotropical region

    Kontschán, J.; Starý, Josef

    2013-01-01

    Roč. 37, č. 1 (2013), s. 7-14. ISSN 1300-0179 Institutional support: RVO:60077344 Keywords : Acari * Uropodina * Trachyuropoda * Galápagos Islands Subject RIV: EG - Zoology Impact factor: 0.585, year: 2013

  6. Comparison and Characterization of Garlic (Allium sativum L. Bulbs Extracts and Their Effect on Mortality and Repellency of Tetranychus urticae Koch (Acari: Tetranychidae Comparación y Caracterización de Extractos de Bulbos de Ajo (Allium sativum L. y su Efecto en a Mortalidad y Repelencia de Tetranychus urticae Koch (Acari: Tetranychidae

    Carlos Augusto Hincapié Ll

    2008-12-01

    Full Text Available Tetranychus urticae Koch is a pest that causes great economic losses because it attacks a wide range of host plants. Also, some populations have developed resistance against commercial acaricides. This work compares different extracts from garlic (Allium sativum L. bulbs measuring their toxicity and repellency effects on T. urticae. Extracts were obtained using as solvents CO2 in supercritical conditions (CSC, ethanol and petroleum ether using soxhlet and soaking methods and soaking in water. The supercritical fluid extraction technique allows for obtaining extracts at low temperature using high pressures, avoiding compound degradation and making possible solvent separation by exposing the extract at room temperature. Mites were bred on bean plants (Phaseolus vulgaris L. in a laboratory environment in Laureles Campus, Universidad Pontificia Bolivariana, Medellín, Colombia. Mortality was evaluated putting 10 mites in P. vulgaris 3 cm diameter leaf discs previously submersed in the extract. The lowest mean lethal concentrations (LC50 were obtained with the CSC method (8.1188, 5.4105, 2.8206 mg g-1 at 24, 48 and 72 h, respectively. Extracts were characterized using gas chromatography/mass spectrometry (GC/MS, finding vinyl dithiins (63.11%, diallyl disulfide (10.88% and diallyl trisulfide (10.4% as the main compounds in the CSC extract. The main conclusion is that, in comparison with other techniques of extraction from garlic bulbs used in this study, supercritical fluids allow for obtaining extracts with a higher concentration of biologically active compounds against T. urticae.Tetranychus urticae Koch es una plaga que causa grandes pérdidas económicas porque ataca un numeroso grupo de cultivos. Además, algunas poblaciones han desarrollado resistencia a acaricidas comerciales. Este trabajo compara diferentes extractos a partir de bulbos de ajo (Allium sativum L. a través de su toxicidad y repelencia sobre T. urticae. Los extractos se obtuvieron usando como solventes CO2 en condiciones supercríticas (CSC, etanol y éter de petróleo usando el método soxhlet y maceración y agua usando maceración. La técnica de fluidos supercríticos permite obtener extractos a temperaturas bajas usando altas presiones, evitando la degradación de los compuestos y permitiendo su separación del solvente al exponerlo a temperatura ambiente. El ácaro fue criado en laboratorio sobre plantas de fríjol (Phaseolus vulgaris L. en el Campus Laureles de la Universidad Pontificia Bolivariana, Medellín, Colombia. La mortalidad fue evaluada colocando 10 mites en un disco de hoja de P. vulgaris de 3 cm de diámetro previamente sumergido en el extracto. Las concentraciones letales medias (CL50 más bajas se obtuvieron con el método CSC (8.1188, 5.4105, 2.8206 mg g-1 a las 24, 48 y 72 h respectivamente. Los extractos se caracterizaron usando cromatografía de gases-espectrometría de masas (GC/MS, obteniendo en el extracto CSC principalmente vinillditiínas (63,11%, dialil disulfuro (10,88% y di-2-propenil trisulfuro (10,4%. Se concluyó que la extracción con fluidos supercríticos permite obtener, a partir de bulbos de ajo, extractos con mayor concentración de compuestos biológicamente activos contra T. urticae, comparada con las otras técnicas usadas en este estudio.

  7. Comparison and Characterization of Garlic (Allium sativum L.) Bulbs Extracts and Their Effect on Mortality and Repellency of Tetranychus urticae Koch (Acari: Tetranychidae) Comparación y Caracterización de Extractos de Bulbos de Ajo (Allium sativum L.) y su Efecto en a Mortalidad y Repelencia de Tetranychus urticae Koch (Acari: Tetranychidae)

    Carlos Augusto Hincapié Ll; Gloria Eugenia López P; Ricardo Torres Ch

    2008-01-01

    Tetranychus urticae Koch is a pest that causes great economic losses because it attacks a wide range of host plants. Also, some populations have developed resistance against commercial acaricides. This work compares different extracts from garlic (Allium sativum L.) bulbs measuring their toxicity and repellency effects on T. urticae. Extracts were obtained using as solvents CO2 in supercritical conditions (CSC), ethanol and petroleum ether using soxhlet and soaking methods and soaking in wate...

  8. Eriophyoid mites from Northeast China (Acari: Eriophyoidea).

    Xue, Xiao-Feng; Guo, Jing-Feng; Hong, Xiao-Yue

    2013-01-01

    We describe and illustrate herein one new genus and eighteen new eriophyoid mite species (Acari: Eriophyoidea) collected in northeast China. They are: Shevtchenkella huzhongiensis sp. nov. on Ulmus davidiana Planch. var. japonica (Sarg. ex Rehder) Nakai (Ulmaceae), Shevtchenkella jingboicus sp. nov. on Acer sp. (Aceraceae), Calepitrimerus flexuosus sp. nov. on Spiraea flexuosa Fisch. ex Cambess. (Rosaceae), Calepitrimerus maximowiczii sp. nov. on Crataegus maximowiczii Schneid. (Rosaceae), Calepitrimerus pilosus sp. nov. on Agrimonia pilosa Ledeb. (Rosaceae), Calepitrimerus yichunensis sp. nov. on Sorbaria sorbifolia (L.) A.Br. (Rosaceae), Cupacarus oxyphyllus sp. nov. on Euonymus oxyphyllus Miq. (Cel-astraceae), Epitrimerus sambucus sp. nov. on Sambucus williamsii Hance (Caprifoliaceae), Epitrimerus wuyingensis sp. nov. on Acer sp. (Aceraceae), Longisolenidionus amurensis gen. nov & sp. nov. on Tilia amurensis Rupr. (Tiliaceae), Phyllocoptes jiagedaqiensis sp. nov. on Cunninghamia sp. (Taxodiaceae), Aculops huzhongensis sp. nov. on Salix sp. (Sali-caceae), Aculus huzhongsalixus sp. nov. on Salix sp. (Salicaceae), Tetra angelica sp. nov. on Angelica sp. (Apiaceae), Tetra jiagedaqia sp. nov. on Lespedeza sp. (Fabaceae), Vittacus mandshurica sp. nov. on Corylus sieboldiana Blume var. mandshurica (Maxim.) C. K. Schneid. (Betulaceae), Vittacus cannabus sp. nov. on Cannabis sativa L. (Moraceae), and Peralox dentatis sp. nov. on Ulmus sp. (Ulmaceae). Two species formerly assigned to Rhyncaphytoptus, R. abiesis (Xue, Song & Hong, 2006) and R. fabris (Xue, Song & Hong, 2006) were reassigned to Nalepella, based on the presence of seta vi on the apical shield, and other characteristics of Nalepella. One species formerly assigned to Rhyncaphytoptus, R. fargesis (Xue, Song & Hong, 2006) was reassigned to Pentaporca, based on the presence of seta vi on the apical shield, opisthosoma with five ridges and other characteristics of Pentaporca. At the same time, four new eriophyoid

  9. Can Euseius alatus DeLeon (Acari: Phytoseiidae) prey on Aceria guerreronis Keifer (Acari: Eriophyidae) in coconut palm?

    Mites of the genus Euseius are generally considered specialist as pollen feeders. Euseius alatus DeLeon is one of the six species of phytoseiid mites most commonly found on coconut plants in northeast Brazil associated with Aceria guerreronis Keifer. Although the morphology of E. alatus does not favor the exploitation of the meristematic area of the fruit inhabited by A. guerreronis, the predator may have some role in the control of this eriophyid during the dispersion process. The objective of this work was to evaluate the development and reproduction of E. alatus on the following diets: A. guerreronis, Ricinus communis pollen (Euphorbiaceae), and Tetranychus urticae Koch (Tetranychidae) + R. communis pollen + honey solution 10%. Euseius alatus developed slightly faster and had slightly higher oviposition rate when feeding on the diet composed of T. urticae + pollen + honey. However, life table parameters were very similar on all diets, suggesting that E. alatus may contribute in reducing the population of A. guerreronis in the field. (author)

  10. Estudos taxonômicos de ácaros Tetranychidae no Brasil e filogenia e estrutura genética do ácaro rajado, Tetranychus urticae Koch, inferidas a partir de sequências do DNA ribossômico e mitocondrial : Études taxonomiques des acariens Tetranychidae au Brésil, en particulier sur la phylogenie et la structure genetique des populations de l´acarien jaune, Tetranychus urticae Koch, inferées à partir des sequences d´AND ribosomique et mitochondrial

    de Mendonça, Renata

    2010-01-01

    The Tetranychidae family or spider mites comprises a large group of phytophagous mites with many of its species considered as important pests of agricultural crops worldwide. The two-spotted spider mite, Tetranychus urticae Koch (Prostigmata: Tetranychidae), an ubiquitous species causing outbreaks in many crops, is certainly the most studied species. Many aspects of its systematic, biology, feeding habits, and control have been studied. In Brazil, T. urticae is considered as one of the three ...

  11. Gamma irradiation as a quarantine treatment for spider mites (Acarina: tetranychidae) in horticultural products

    The carmine spider mite, Tetranychus cinnabarinus (Boisd.), and the two-spotted spider mite, Tetranychus urticae Koch, are closely related species of tetranychid mites (Acarina, Tetranychidae) that respond to gamma irradiation in a similar way. Eggs of both species exposed to gamma radiation early in embryonic development were considerably more susceptible to irradiation than older eggs. The tolerance of eggs to gamma radiation increased in 3-4-day-old eggs, when eye-spots were formed. Nymphs were more resistant to gamma radiation than eggs and larvae. Deteriorative effects of irradiation treatment were reflected in the immatures by their mortality in subsequent developmental stages. A positive relationship between dosage and the percent egg mortality or the mortality of subsequent stages was usually found when the immature stages were irradiated. The sex ratio of adults developed from irradiated eggs, larvae, and nymphs was affected by the irradiation treatment; the ratio was usually skewed towards males. Irradiation of females resulted in increased mortality, lowered fecundity, reduced egg viability, and sex ratio distortion in their progeny. Two-day-old females of the carmine spider mite and the two-spotted spider mite irradiated with 200 or 300 Gy lived as long as the controls. Mortality occurred after 3 weeks. The number of eggs laid by irradiated females of spider mites was considerably lower than in the control, and it decreased as the absorbed dose increased. The higher the dose of gamma radiation applied to adults of the spider mites (the parental generation, P), the higher the mortality of the F1 mites during their embryonic development. Viability of eggs laid by irradiated females of spider mites mated with irradiated males was significantly reduced. Young females treated with a dose of 0.2 kGy produced 40-50% nonviable eggs, while control mites produced only 6.0-6.6% nonviable eggs. A dose of 0.3 kGy caused high mortality of eggs; 88% and 97% nonviable

  12. Seletividade de produtos fitossanitários sobre o ácaro predador Agistemus brasiliensis Matioli, Ueckermann & Oliveira (Acari: Stigmaeidae Selectivity ofthe pesticides tothe predaceous mite Agistemus brasiliensis Matioli,, Ueckermann & Oliveira (Acari: Stigmaeidae

    Marcos Zatti da Silva

    2009-06-01

    Full Text Available Os ácaros predadores das famílias Phytoseiidae e Stigmaeidae constituem-se nos principais inimigos naturais de Brevipalpus phoenicis (Geijskes em citros. Este ácaro-praga causa sérios prejuízos na produção, devido à transmissão do vírus da leprose dos citros (CiLV. Apesar do grande volume de informações sobre a sensibilidade de ácaros Phytoseiidae a agrotóxicos, praticamente não existem informações sobre o efeito desses compostos em ácaros Stigmaeidae no Brasil. Sendo assim, o trabalho teve por objetivo avaliar o efeito dos principais agrotóxicos utilizados em citros sobre o ácaro predador Agistemus brasiliensis Matioli, Ueckermann & Oliveira (Acari: Stigmaeidae, em condições de laboratório. Arenas de folhas de citros da variedade Pera, contendo 25 fêmeas adultas de A. brasiliensis, foram pulverizadas em torre de Potter. Avaliaram-se as mortalidades dos ácaros 72 horas após a aplicação. O efeito dos produtos na reprodução do acarino e a viabilidade dos ovos também foram avaliados. Quanto à seletividade, conforme proposta da "Organização Internacional para o Controle Biológico" (IOBC, os produtos foram classificados como: classe 1 - inócuo (E99%, calda sulfocálcica, cyhexatin, flufenoxuron, hexythiazox, óxido de fenbutatin, propargite, pyridaben e spirodiclofen. Estudos conduzidos em condições de campo ainda são necessários para se compreender melhor o efeito desses agrotóxicos sobre o ácaro predador.The predaceous mites of the families Phytoseiidae and Stigmaeidae are the most important natural enemies of Brevipalpus phoenicis (Geijskes on citrus. This mite causes serious damages to the yield due to the transmission of Citrus leprosis virus (CiLV. Despite the considerable amount of information on susceptibility of phytoseiids to pesticides, the effect of these compounds is not very known for stigmaeid mites in Brazil. This work was carried out to evaluate the effect of the main pesticides used in

  13. The complete mitochondrial genome of the scab mite Psoroptes cuniculi (Arthropoda: Arachnida) provides insights into Acari phylogeny

    Gu, Xiao-Bin; Liu, Guo-Hua; Song, Hui-Qun; Liu, Tian-Yu; Yang, Guang-You; Zhu, Xing-Quan

    2014-01-01

    Background Limited available sequence information has greatly impeded population genetics, phylogenetics and systematics studies in the subclass Acari (mites and ticks). Mitochondrial (mt) DNA is well known to provide genetic markers for investigations in these areas, but complete mt genomic data have been lacking for many Acari species. Herein, we present the complete mt genome of the scab mite Psoroptes cuniculi. Methods P. cuniculi was collected from a naturally infected New Zealand white ...

  14. Variability in Damage Caused by the Mite Tetranychus urticae (Trombidiformes: Tetranychidae) Koch on Three Varieties of Strawberry.

    González-Domínguez, S G; Santillán-Galicia, M T; González-Hernández, V; Suárez Espinosa, J; González-Hernández, H

    2015-06-01

    The strawberry, Fragaria×ananassa Duchesne (Rosales: Rosaceae), is an important crop in Mexico. We evaluated the tolerance of three newly developed Mexican strawberry varieties (CP0615, CPLE-7, and CPJacona) to Tetranychus urticae Koch (Trombidiformes: Tetranychidae), the most important pest of strawberry. We evaluated the effect of three different initial mite densities on population growth, duration of each developmental stage and survival of T. urticae on the three strawberry varieties. We also compared the photosynthetic activity (Pn), sub-stomatal CO2 concentration (Ci), stomatal conductance (gs) and the area of leaf damaged in the three varieties. The largest final density of mites occurred on the variety CP0615, followed by the varieties CPLE-7 and CPJacona. There were no significant differences in the duration of T. urticae developmental stages amongst the varieties, except for larvae where the shortest duration was on variety CPLE-7. The proportion of eggs reaching the adult stage (survival) was significantly lower on the variety CPLE-7. The number and morphology of the trichomes did not play an important role in the outcomes, as they were similar in the three varieties. There were no significant differences in Pn, Ci, and gs values amongst the three varieties in the presence and absence of T. urticae. The area of leaf damaged in variety CPLE-7 was significantly smaller than for the other varieties. Based on these results, and with regard to spider mite tolerance, we believe that the variety CPLE-7 has the greatest potential for further development, and eventually, for use on a commercial scale in Mexico. PMID:26470266

  15. A new pomegranate pest for Turkey; Pomegranate false spidermite, Tenuipalpus punicae Pirtchard and Baker (Acari: Tenuipalpidae)

    DÖKER, İsmail; KAZAK, Cengiz; KARUT, Kamil

    2013-01-01

    In this study, the pomegranate false spider mite Tenuipalpus punicae Pirtchard and Baker (Acari: Tenuipalpidae) collected from pomegranate growing areas of Adana province is determined for the first record for Turkish mite fauna. Some information related to world distribution, damage, biology and control of this pest are presented in the paper.

  16. Stigmaeus ceylani, a new species of the genus Stigmaeus Koch (Acari: Stigmaeidae) from Turkey

    Uluçay, İsmail

    2015-01-01

    In this study a new species of Stigmaeus Koch (Acari: Stigmaeidae), Stigmaeus ceylani sp. nov., is described and illustrated. Specimens have been collected from the soil and litter under Punica granatum, 2014, İskenderun, Hatay, Turkey. Besides, the male, female, deutonymph, protonymph and larva of the species are illustrated in this article.  

  17. Investigations of Gamasina mites in natural and man-affected soils in Latvia (Acari: Mesostigmata)

    Salmane, I.

    2003-01-01

    Investigations of Gamasina mites in natural and man-affected soils in Latvia (Acari: Mesostigmata) A short overview is presented on Gamasina material collected in 22 natural and man-disturbed habitats in Latvia. Species diversity, average density and species dominance were investigated. Altogether 1

  18. Recharacterization of Gymnodamaeoidea and erection of Plateremaeoidea (Acari, Oribatei), with key to families and genera

    Adilson D. Paschoal

    1989-01-01

    The present paper deals with the recharacterization of the Gymnodamaeoidea and the erection of Plateremaeoidea n. grad. (Acari, Oribatei). Gymnodamaeidae Grandjean, Aleurodamaeidae Paschoal & Johnston, and Idiodamaeidae Paschoal are recognised under the Gymnodamaeoidea. Plateremaeidae Tragardh, Pheroliodidae Paschoal, Nooliodidae Paschoal, Hammeriellidae Paschoal, Lyrifissellidae Paschoal, Pedrocortesellidae Paschoal, Licnodamaeidae Grandjean and Licnobelbidae Grandjean are included in Plater...

  19. Seletividade de agrotóxicos usados na cultura da macieira a Neoseiulus Californicus (McGregor (Acari: Phytoseiidae1 Pesticide selectivity used in apple crops Neoseiulus californicus (McGregor (Acari: Phytoseiidae

    Geraldine de Andrade Meyer

    2009-06-01

    Full Text Available Objetivou-se avaliar os efeitos secundários dos principais agrotóxicos utilizados em macieira sobre adultos e imaturos de Neoseiulus californicus (McGregor (Acari: Phytoseiidae. Os testes foram conduzidos em laboratório, utilizando as doses dos produtos recomendadas para a cultura e o método de contato e residual com pulverização em superfície de folha. Foram testados tebufenozida, fosmete, metidationa, clorpirifós, abamectina, fenpiroximato, piridabem, captana, mancozebe (duas dosagens e ditianona. Para o cálculo do efeito total (E% sobre os adultos, avaliaram-se a mortalidade, a oviposição e a viabilidade dos ovos, e para os imaturos, somente a mortalidade. Os resultados do E% foram avaliados 96 horas após a pulverização. Os produtos foram classificados quanto ao efeito total (E% de toxicidade proposta pela IOBC/WPRS. Fosmete, tebufenozida e metidationa foram inócuos; abamectina, fenpiroximato, clorpirifós, captana, mancozebe (nas duas dosagens testadas e ditianona foram levemente nocivos, e piridabem foi moderadamente nocivo aos adultos de N. californicus. O fungicida mancozebe, na maior dosagem (320g,i.a./100L, foi o que mais afetou o ácaro predador. Quanto à seletividade dos agrotóxicos aos imaturos, constatouse que abamectina e piridabem foram moderadamente nocivos, e os demais foram inócuos. Nenhum produto foi classificado como nocivo, evidenciando a tolerância de N. californicus a estes agrotóxicos. Estes resultados permitem uma escolha e manejo mais adequado para os agrotóxicos utilizados nos pomares comerciais de macieira, de forma que a presença deste ácaro predador exerça pressão de controle do ácaro-vermelho.The objective of this study was to evaluate the side effects of the main pesticides used in adult and immature Neoseiulus californicus (McGregor (Acari: Phytoseiidae. The tests were carried out in the laboratory by using the contact and residual spraying method on the leaf surface. It was tested

  20. Garrapatas (Acari: Ixodidae) asociadas a perros en diferentes ambientes de la provincia de Corrientes, Argentina Ticks (Acari: Ixodidae) of dogs in different environments of the Corrientes Province, Argentina

    V.N. Debárbora; E.B. Oscherov; A.A. Guglielmone; Nava, S.

    2011-01-01

    Se estudiaron las garrapatas (Acari: Ixodidae) de perros en ambientes urbanos, periurbanos y rurales de la provincia de Corrientes por medio de colecciones mensuales durante un año en siete sitios diferentes. De los 138 perros examinados, en 87 (63,04%) se determinaron tres especies de garrapatas: Amblyomma tigrinum (n=35), Amblyomma ovale (n=2) y Rhipicephalus sanguineus sensu lato (n=523). Las mayores prevalencias para A. tigrinum y R. sanguineus s.l. fueron halladas en ambientes rurales. E...

  1. Five New Records of Raphignathoid Mites (Acari: Raphignathoidea from Poland/ Pięć Nowych Gatunków Roztoczy Z Nadrodziny Raphignathoidea (Acari Stwierdzonych W Faunie Polski

    Doðan Salih

    2014-12-01

    Full Text Available Pięć nowych gatunków roztoczy (Acari: Raphignathoidea zostało odnoto- wanych jako nowe dla fauny Polski: trzy z rodziny Stigmaeidae, Eustigmaeus rhodomela (Koch, Mediolata obtecta Dónel et Doóan, Stigmaeus glabrisetus Summers, jeden z rodziny Cryptognathidae, Favognathus cucurbita (Berlese, i jeden z rodziny Barbutiidae, Barbuda anguineus (Berlese. Ostatni z wymienio- nych jest jednocześnie pierwszym stwierdzeniem przedstawiciela rodziny Barbutiidae w Polsce.

  2. Molecular analysis of Boophilus spp. (Acari: Ixodidae) tick strains.

    Fuente, J; García-García, J C; González, D M; Izquierdo, G; Ochagavia, M E

    2000-10-01

    Boophilus spp. (Acari: Ixodidae) parasitize cattle and other farm and wild animals in tropical and subtropical regions of the world. Ticks belonging to the genus Boophilus have undergone evolutionary processes associated with habitat adaptation following biogeographical separation, resulting in strains with marked morphological differences. We have characterized at the molecular level B. microplus strains from Latin America and Australia, employing sequences derived from the bm86 coding region, an intron located within the bm86 gene, and DNA short tandem repeats (STR). A B. annulatus strain was employed for comparison. The results indicated that variation within the bm86 coding region is higher between B. microplus strains than between some B. microplus strains and B. annulatus. The sequence of the intron was not informative for phylogenetic analysis, varying among individuals of the same strain. Two STRs were identified in B. microplus (STRs BmM1 and BmM2) and one in B. annulatus (STR Ba1). Southern hybridization experiments with STRs BmM1 and BmM2 as a probe revealed the prevalence of dispersed moderately repeated DNA in the genome of B. microplus. The analysis of polymorphism at STR locus BmM1 evidenced differences within and between populations of B. microplus. These results support at the molecular level the existing differences between B. microplus strains and suggest tools to characterize these populations. PMID:10962158

  3. New Wolbachia supergroups detected in quill mites (Acari: Syringophilidae).

    Glowska, Eliza; Dragun-Damian, Anna; Dabert, Miroslawa; Gerth, Michael

    2015-03-01

    Wolbachia is the most abundant intracellular bacterial genus infecting a wide range of arthropods and filarial nematodes. Wolbachia have evolved parasitic, mutualistic and commensal relationships with their hosts but in arthropods generally act as reproductive parasites, inducing a wide range of phenotypic effects such as cytoplasmic incompatibility, parthenogenesis, feminization and male-killing. Up to now, the genus has been divided into 14 supergroups successively named A-O. Here, we describe two new Wolbachia supergroups from syringophilid mites (Acari: Cheyletoidea). These obligatory ectoparasites of birds inhabit the quills of feathers in many avian groups. The species of this family reproduce in a haplodiploid mode sensu arrhenotoky and are usually strongly female-biased. Based on the sequences of four protein-coding genes (ftsZ, gltA and groEL and coxA) and the 16S rRNA we identified strains of three Wolbachia supergroups (F and two distinct, yet undescribed ones) in five quill mite species. Our results suggest that in some cases the distribution of the bacteria can be better correlated with the mite's bird host rather than with mite taxonomy as such. The discovery of two new Wolbachia supergroups not only broadens the knowledge of the diversity of this bacterium but also raises questions about potential effects induced in quill mites and transmission mechanisms of the endosymbionts in this peculiar bacteria-quill mite-bird system. PMID:25541519

  4. A rapid and non-destructive method to assess leaf injury caused by the cassava green mite, <em>Mononychellus tanajoaem> (Bondar) (Acarina: Tetranychidae)

    Tomkiewicz, Jonna; Skovgård, Henrik; Nachman, Gösta;

    1993-01-01

    A relative scale of Leaf Damage Indices (LDI) from 0 to 5 describes the visible injury to leaves of cassava, Manihot esculenta Crantz caused by the cassava green mite, Mononychellus tanajoa (Bondar). As the scale is ordinal and thus not quantitative, the observed LDIs are converted individually to...

  5. 巴氏钝绥螨对柑桔全爪螨的捕食效能%Functional Response of Amblyseius barkeri (Acarina: Phytoseiidae) on Panonychus citri (Acari: Tetranychidae)

    凌鹏; 夏斌; 李朋新; 舒畅; 钟玲; 李爱华

    2008-01-01

    本文研究了巴氏钝绥螨Amblyseius barkeri的雌成螨、雄成螨、若螨在实验条件下对柑桔全爪螨的捕食效能.结果表明:5个实验温度条件下(16、20、24、28和32℃,RH85%),利用椭圆食粉螨饲养的巴氏钝绥螨Panonychus citri对柑桔全爪螨均有较强的捕食能力;巴氏钝绥螨对柑桔全爪螨的功能反应均属于Holling Ⅱ型.在实验温度范围内,各螨态对柑桔全爪螨的控制能力(α/Tb)值)随温度升高而增大,在28℃时达到峰值;同一温度条件下,雌成螨的捕食能力最大,若螨其次,雄成螨的捕食能力最弱;在猎物密度固定时,由于巴氏钝绥螨个体之间存在相互竞争和相互干扰作用,随着捕食者密度的提高,捕食者平均捕食量逐渐减少.巴氏钝绥螨对各螨态柑桔全爪螨的取食有选择性,柑桔全爪螨的幼若螨是巴氏钝绥螨的嗜好虫态.

  6. Relationship between metabolism genes and amitraz resistance of Panonychus citri (Acari: Tetranychidae)%几种代谢酶基因与柑橘全爪螨对双甲脒抗性的关系

    陈飞; 张云飞; 刘浩强; 李鸿筠; 冉春

    2013-01-01

    为了明确羧酸酯酶(carboxylesterase,CarE)基因、谷胱甘肽S-转移酶(glutathione S-transferases,GST)基因和过氧化氢酶(catalase,CAT)基因与柑橘全爪螨Panonychus citri对双甲脒抗性的关系,通过BLAST检索,从柑橘全爪螨转录组数据库中对这3种代谢酶抗性相关基因进行鉴定,并采用RPKM法对双甲脒抗性品系和敏感品系代谢抗性相关基因进行表达差异分析,对差异较大的基因作定量PCR检测.基因差异性分析发现,抗性品系中有9条CarE基因、12条GST基因及6条CAT基因表达量发生上调,13条CarE基因、12条GST基因和3条CAT基因表达量发生下调;Pc29773nrt、Pcl7807nlg和Unigene31477为上调倍数最高的3个基因,其log2 ratio (RS/SS)分别为12.95、10.81、10.01.定量分析显示,Pc29773 nrt、Pcl7807nlg和Unigene31477的上调倍数分别为3.72、2.03和3.09,Pc29773 nrt和Unigene31477上调显著.研究表明柑橘全爪螨Pc29773nrt和Unigene31477上调与其对双甲脒的抗性相关.

  7. Sublethal effects of scopoletin on the experimental population of the carmine spider mite, Tetranychus cinnabarinus(Boisduval)(Acari:Tetranychidae )%东莨菪内酯对朱砂叶螨实验种群的亚致死效应

    雍小菊; 张永强; 丁伟

    2011-01-01

    为明确东莨菪内酯对朱砂叶螨Tetranychus cinnabarinus(Boisduval)实验种群的亚致死效应,为其作为植物源杀螨剂的进一步开发及应用提供理论依据,在室内采用叶碟饲养的方法,并利用生命表技术研究了东莨菪内酯亚致死剂量对朱砂叶螨F0代和F1代种群生长发育和繁殖的影响.结果表明:东茛菪内酯LC40,LC30和LC20剂最处理朱砂叶螨雌成螨后,可刺激F0代雌成螨产卵,使产卵量分别增加了73.82%,186.97%和70.18%,而对成螨寿命、产卵期和雌雄性比没有影响;F1代的卵期、幼螨期和若螨期与对照没有显著性差异(P>0.05),而成螨期和雌成螨寿命均低于对照;对F1代繁殖力的影响主要表现为产卵期缩短、产卵量减少和雌性比下降;子代种群的净生殖率(R0)由50.5976最低降至33.9910,世代平均历期(T和种群倍增时间(Dt)均低于对照,各处理后的朱砂叶螨种群均存在适合度缺陷.这些结果表明,在亚致死剂量下,东莨菪内酯能够降低朱砂叶螨种群的发育和繁殖速率,这对东莨菪内酯在有害生物防治上的应用具有积极意义.%In order to clarify the sublethal effects of scopoletin on the experimental population of Tetranychus cinnabarinus (Boisduval), and provide the theoretical basis for the further development and application of scopoletin used as a plant-derived acaricide, we used the leaf disc bioassay to evaluate the effects of scopoletin at sublethal dosages on F0 and F1 populations. The results showed that the fecundity of female adults treated with scopoletin at three sublethal dosages (LC40, LC30 and LC20,) was increased by 73. 82% , 186.97% and 70. 18% , respectively, while the longevity, oviposition duration and frmale-to-male sex ratios were not affected. The duration of oviposition, larval and nymphal stages was not significantly different from that of the control (P>0.05) , but the adult period and female longevity were shorter than those of the control. The F, generation displayed shortened oviposition duration, reduced fecundity and decreased the proportion of females when compared with the control. The net reproductive rate (R0) dropped from 50.5976 to the lowest 33.9910, the mean generation time ( T) and population doubling time (Dt) were all shorter than those of the control, and fitness defects appeared in all three treatment populations. The results indicate that scopoletin treatment at sublethal dosages can reduce the development and reproduction rates of T. Cinnabarinus populations, and this provides positive evidence for application of scopoletin in pest management.

  8. Reproductive effects and localization of Wolbachia and Cardinium in the spider mite Tetranychus piercei ( Acari: Tetranychidae)%Wolbachia和Cardinium对皮氏叶螨生殖的影响及在寄主体内的定位

    朱路雨; 蒋欣雨; 杨思霞; 徐敏; 洪晓月

    2012-01-01

    Wolbachia和Cardinium均为母系遗传的胞内共生菌,它们能够通过诱导胞质不亲和(cytoplasmic incompatibility,CI)以调控寄主的生殖.目前,关于Wolbachia和Cardinium共同对同一寄主进行生殖操控的机制还不清楚.本研究以皮氏叶螨Tetranychus piercei McGregor广州种群为实验材料,通过杂交实验和荧光原位杂交的方法,研究Wolbachia和Cardinium单感染和双感染对寄主生殖的影响.结果表明:单感染Wolbachia诱导较弱的CI,不亲和组合的未孵化率为17.8%±1.6%.单感染Cardinium及双感染Wolbachia和Cardinium能诱导高强度的CI,不亲和组合的未孵化率分别为70.3%±1.3%和72.9%±1.2%.同时双感染Wolbachia和Cardinium雌螨的平均产卵量为35.2±1.2,显著高于单感染和不感染的雌螨的产卵量.Wolbachia和Cardinium分别诱导以及共同诱导CI的水平与精子形成过程中的感染情况有关.Wolbachia和Cardinium的垂直传播模式结果显示,在卵的不同发育阶段,Wolbachia和Cardinium主要伴随着营养物质从滋养细胞、中肠、输卵管进入发育中的卵.研究结果为进一步了解Wolbachia和Cardinium的母系遗传机制提供了重要依据.%Wolbachia and Cardinium, with the ability to induce cytoplasmic incompatibility ( CI) , are maternally inherited intracellular bacteria known to manipulate the reproduction of their hosts. The exact mechanisms of CI which is induced by these two endosymbionts in the same host are unknown. This study tried to investigate the reproductive manipulation of Wolbachia or/ and Cardinium infected spider mite Tetranychus piercei McGregor by crossing experiment and fluorescence in situ hybridization ( FISH). The results indicated that Wolbachia-infected males induced weak CI. In Guangzhou population of the spider mite, approximate 17. 8% ± 1.6% of all eggs did not hatch in the incompatible cross U/Iw. Cardinium-infected and Wolbachia and Cardinium doubly infected males caused severe CI. The unhatched eggs in the incompatible cross U/Ic and U/Iwc accounted for 70. 3% ±1. 3% and 72. 9% ± 1. 2% , respectively, the number of eggs laid per doubly infected female was 35. 2 ±1.2, significantly higher than that of singly ( Wolbachia or Cardinium) infected female. There is a positive correlation between CI levels and infection status of the developing sperm. Wolbachia and Cardinium mainly enter the oocytes with trophic flow from nurse cells, midgut and oviduct. The results might provide foundation for understanding the maternally inherited mechanisms of Wolbachia and Cardinium.

  9. Effect of Extreme High Temperature on the Enzymes of Cassava Green Mite Mononychellus tanajioa(Acari: Tetranychidae)%极端高温对木薯单爪螨保护酶活性的影响研究

    卢芙萍; 符悦冠; 郭容琦; 蔡源; 经福林; 徐雪莲; 卢辉; 陈青

    2012-01-01

    为探讨木薯单爪螨的生态适应性机理,揭示其种群扩散蔓延机制.开展了极端高温胁迫对木薯单爪螨各龄螨的存活率及几种保护酶(多酚氧化酶(PPO)、过氧化物酶(POD)、抗坏血酸过氧化物(AsA-POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD))活性的影响研究.结果表明:经42℃高温处理,木薯单爪螨各龄螨最多仅可存活66 h,不能进一步发育.PPO和CAT活性在幼螨体内显著下降,而在前若螨到成螨却显著升高,而尤以前若螨升高最高;与对照相比,AsA-POD活性在幼螨体内无变化,但在前若螨体内可升高达对照的3.286倍,到后若螨期升高至对照的1.923倍,成螨期无显著变化;各龄螨体内POD和SOD的活性均无显著变化.因此,说明PPO、CAT和AsA-POD活性变化可能在木薯单爪螨抵抗高温过程中具有重要作用.%Temperature was one of the important ecological factors for development and reproduction of Mononychellus tanajioa. In order to learn about the ecological adaptation of M. Tanajoa after its invasion into our country and provide a foundation for the monitoring early warning and effective prevention as well as analyze its invasion, diffusion and ecological adaptation mechanism, this study researched the effect of extremely high-temperature (42℃) treatment on the survive and the activity changes of several protective enzymes [polyphenol oxidase (PPO), peroxidase (POD), ascorbate oxidase (AsA-POD), catalase (CAT) and superoxide dismutase (SOD)] by spectrophotometric endpoint assay after the mites in each growth period were treated for different lengths of time at 42℃. The results showed that 42℃ had important effect on the protective enzymes of M. Tanajioa. Activities of PPO and CAT of larvae significantly decreased under 42℃ treatment, but that of them were increased in protonymphs and adults, especially in protonymphs. In 42℃ , AsA-POD activities of larvae and adults had no changes, but that increased 3.286-fold in protonymphs and 1.923-fold in deutonymph relative to that in 25℃. High temperature of 42℃ had no effect on POD and SOD activities of M. Tanajioa. These results suggested that the tolerance of M. Tanajioa to high temperature could be associated with PPO, CAT and AsA-POD, but not with POD and SOD activities.

  10. Phylogenetic analysis of the spider mite sub-family Tetranychinae (Acari: Tetranychidae based on the mitochondrial COI gene and the 18S and the 5' end of the 28S rRNA genes indicates that several genera are polyphyletic.

    Tomoko Matsuda

    Full Text Available The spider mite sub-family Tetranychinae includes many agricultural pests. The internal transcribed spacer (ITS region of nuclear ribosomal RNA genes and the cytochrome c oxidase subunit I (COI gene of mitochondrial DNA have been used for species identification and phylogenetic reconstruction within the sub-family Tetranychinae, although they have not always been successful. The 18S and 28S rRNA genes should be more suitable for resolving higher levels of phylogeny, such as tribes or genera of Tetranychinae because these genes evolve more slowly and are made up of conserved regions and divergent domains. Therefore, we used both the 18S (1,825-1,901 bp and 28S (the 5' end of 646-743 bp rRNA genes to infer phylogenetic relationships within the sub-family Tetranychinae with a focus on the tribe Tetranychini. Then, we compared the phylogenetic tree of the 18S and 28S genes with that of the mitochondrial COI gene (618 bp. As observed in previous studies, our phylogeny based on the COI gene was not resolved because of the low bootstrap values for most nodes of the tree. On the other hand, our phylogenetic tree of the 18S and 28S genes revealed several well-supported clades within the sub-family Tetranychinae. The 18S and 28S phylogenetic trees suggest that the tribes Bryobiini, Petrobiini and Eurytetranychini are monophyletic and that the tribe Tetranychini is polyphyletic. At the genus level, six genera for which more than two species were sampled appear to be monophyletic, while four genera (Oligonychus, Tetranychus, Schizotetranychus and Eotetranychus appear to be polyphyletic. The topology presented here does not fully agree with the current morphology-based taxonomy, so that the diagnostic morphological characters of Tetranychinae need to be reconsidered.

  11. El ácaro oriental de los cítricos, Eutetranychus orientalis (Klein) (Acari, Tetranychidae), y sus ácaros depredadores, en Andalucía (España)

    Vela López, José Miguel; Ledesma, C.; Wong, Eva; Jacas Miret, Josep Anton; Boyero Gallardo, Juan Ramón

    2013-01-01

    Se ha llevado a cabo un inventario de los tetraníquidos y ácaros depredado- res en huertos de clementina, limonero y naranjo en Andalucía. El ácaro orien- tal, Eutetranychus orientalis, fue la especie dominante tanto en limonero como en naranjo. Otras especies plaga, como Tetranychus urticae y Panonychus citri aparecieron en bajas densidades. Las especies más abundantes de fitoseido fueron Euseius stipulatus y Typhlodromus phialatus, mientras que otros depre- dadores como Neoseiulus californi...

  12. Historical review of the genus Dermanyssus Dugès, 1834 (Acari: Mesostigmata: Dermanyssidae)

    Roy L; Chauve C.M.

    2007-01-01

    A synthetic review of the historical systematics of Dermanyssus Dugès, 1834 (Acari: Mesostigmata: Dermanyssidae) is provided. The classification at the specific level in this early genus has not really been clarified during more than a century despite its economic impact, and the history of the genus is complex and includes various stages. Moreover, Dermanyssus currently includes 23 species, whereas the last review took only 18 species into account. Changes in the species status and position ...

  13. A survey of ticks (Acari: Ixodidae) of companion animals in Australia

    Greay, Telleasha L.; Oskam, Charlotte L.; Alexander W Gofton; Rees, Robert L; Ryan, Una M.; Irwin, Peter J.

    2016-01-01

    Background Ticks are among the most important vectors of pathogens affecting companion animals, and also cause health problems such as tick paralysis, anaemia, dermatitis, and secondary infections. Twenty ixodid species have previously been recorded on dogs, cats, and horses in Australia, including Rhipicephalus sanguineus, Ixodes holocyclus and Haemaphysalis longicornis, which transmit tick-borne diseases. A survey of hard ticks (Acari: Ixodidae) was conducted during 2012–2015 to investigate...

  14. Acaricidal and repellent activities of essential oil of Eucalyptus globulus against Dermanyssus gallinae (Acari: Mesostigmata)

    Dehghani-Samani Amir; Madreseh-Ghahfarokhi Samin; Dehghani-Samani Azam; Pirali-Kheirabadi Khodadad

    2015-01-01

    Introduction: By considering an increase in drug resistance against red mites, finding the nonchemical herbal acaricide against Dermanyssus gallinae (De Geer) (Acari: Mesostigmata) is necessary to kill them and to reduce the chemical resistance against chemical acaricides in this specie. Dermanyssus gallinae is a potential vector of the causal agent of several viral diseases such as Equine encephalitis and St. Louis encephalitis. It can be a vector of bacteria such as Salmonella spp., Mycobac...

  15. A large parasitengonid mite (Acari, Erythraeoidea from the Early Cretaceous Crato Formation of Brazil

    J. A. Dunlop

    2007-08-01

    Full Text Available A new large, fossil mite (Arachnida: Acari, Pararainbowia martilli n. gen. n. sp., is described from the Early Cretaceous (Aptian Crato Formation from Ceará State, Brazil. It is assigned to the Cohort Parasitengona and the superfamily Erythraeoidea, some extant members of which can reach up to seven millimetres in body length. Given that doubts have been raised about the identity of putative Crato feather mite eggs, this new fossil represents the first unequivocal record of Acari from the Crato Formation, the first non-amber record of an erythraeoid mite and the oldest named example of this superfamily. Fossil erythraeoids from Mesozoic and Tertiary ambers are briefly reviewed – including a widely overlooked Late Cretaceous species – with comments on Mesozoic mites in general. Thirteen Baltic amber erythraeoids have been formally described, but much unstudied material from various amber sources remains. Ein neues großes Milbenfossil (Arachnida: Acari, Pararainbowia martilli n. gen. n. sp., wird aus der Crato Formation (Unterkreide, Aptium des Ceará Gebietes in Brasilien beschrieben. Es wird der Kohorte Parasitengona und der Überfamilie Erythraeoidea zugeordnet; die modernen Vertreter erreichen eine Körperlänge bis zu sieben mm. Weil die Identität von Federmilbeneiern aus der Crato Formation in Frage gestellt wurde, ist dieser Neufund der erste klare Hinweis von Acari aus der Crato Formation. Es ist die erste erythraeoide Milbe, die nicht aus dem Bernstein stammt sowie das älteste genannte Beispiel dieser Überfamilie. Fossile erythraeoide Milben aus dem Bernstein des Mesozoikum und des Tertiärs werden kurz zusammengefasst – u. a. eine weitgehend übersehene Art aus der Oberkreide – mit allgemeinen Anmerkungen zu den mesozoischen Milben. Dreizehn erythraeoide Milbenarten sind aus dem baltischen Bernstein genannt und beschrieben worden, aber weiteres unbearbeitetes Material von verschiedenen Bernstein-Fundpunkten liegt noch vor

  16. De corticole fauna van platanen: i. Arachniden (Arachnida: Araneae, Pseudoscorpiones, Acari)

    Noordijk, J.; Berg, M.P.

    2001-01-01

    The corticolous fauna of plane trees: I. Arachnids (Arachnida: Araneae, Pseudoscorpiones, Acari) From February until September 2000 an inventory was made of the bark-dwelling arthropod fauna of more than 400 plane trees (Platanus hybrida), all over the Netherlands. Arthropods were collected from bark and under the ‘loose’ bark fragments at a height of 160-175 cm from the ground. Algae, mosses and fungi are important resources for the corticolous fauna. Crevices in the tree trunk and loose bar...

  17. Mites (Arachnida, Acari on Citrus sinensis L. Osbeck orange trees in the state of Amazonas, Northern Brazil Ácarofauna de Citrus sinensis L. Osbeck no estado do Amazonas, Brasil

    Teiamar da Encarnação Bobot

    2011-01-01

    Full Text Available Despite the importance of citriculture in Brazil, very little is known about mite populations in citrus crops in the Northern Region. In the municipality of Manaus, 12 sprayed sweet orange orchards were surveyed every two weeks during seven months to record mite species amount, and to describe the abundance and distribution of the most important species. The size and age of the orchards varied from 3,360 to 88,080 m² and seven to 25 years, respectively. In the fourteen sampling period, leaves, twigs and fruits were collected from 12 trees, one per orchard. In total, 3,360 leaves, 672 twigs and 1,344 fruits were sampled from 168 trees. Mites were manually extracted from the fruits, and by the washing method on leaves and twigs. We identified pests with the potential to cause economic loss. Fourteen species of phytophagous and mycophagous mites from Eriophyidae, Tarsonemidae, Tenuipalpidae, and Tetranychidae were recorded. Brevipalpus phoenicis (Geijskes 1939 and Phyllocoptruta oleivora (Ashm., 1879, the two commonest phytophagous mites in other Brazilian regions were dominant, showing that local orchards are susceptible to their infestation. Eleven predatory mites were recorded, comprising 10% of the mite population, belonging to Phytoseiidae and Ascidae. Phytoseiidae was the richest family, with ten species. The results are discussed in relation to the temporal variation aspects and habitat use of the most important species. Long-term research encompassing chemical applications followed by evaluations of the mite community are necessary for a better management of the orchards, taking into consideration the seasonal phenology of key pests.Apesar da importância da citricultura no Brasil, pouco se conhece sobre as populações de ácaros em plantações de citros no norte do país. No município de Manaus, 12 pomares de laranja doce pulverizados foram avaliados a cada duas semanas, durante sete meses, para o registro de ácaros plantícolas e

  18. A new species of Atractides Koch, 1837 (Acari: Hydrachnidia, Hygrobatidae from Ethiopia, with a discussion on the biodiversity of the genus Atractides in the Afrotropical region

    Vladimir Pesic

    2011-03-01

    Full Text Available A new species of Atractides Koch, 1837 (Acari, Hydrachnidia is described from Ethiopia. The world number of Atractides now tallies 297 species. The diversity of the genus Atractides in the Afrotropical region is briefly discussed.

  19. New feather mites of the subfamily Pterodectinae (Acari: Astigmata: Proctophyllodidae) from passerines (Aves: Passeriformes) in Mato Grosso do Sul, Brazil

    Mironov, S. V.; Literák, I.; Čapek, Miroslav

    2008-01-01

    Roč. 1947, - (2008), s. 1-38. ISSN 1175-5326 Institutional research plan: CEZ:AV0Z60930519 Keywords : Acari * Astigmata * feather mites * systematics * Brazil * Proctophyllodidae * Aves * Passeriformes Subject RIV: EG - Zoology Impact factor: 0.740, year: 2008 http://www.mapress.com/zootaxa/2008/f/zt01947p038.pdf

  20. A novel disease affecting the predatory mite Phytoseiulus persimilis (Acari, Phytoseiidae): evidence for the involvement of bacteria

    Schütte, C.; Poitevin, O.J.L.; Dicke, M.

    2008-01-01

    Adult female Phytoseiulus persimilis Athias-Henriot (Acari, Phytoseiidae) of a laboratory population show drastic changes in foraging behavior, anatomy and life history compared to typical laboratory populations. We demonstrated earlier that the set of characteristic symptoms, called non-responding

  1. Effect of coconut palm proximities and Musa spp. germplasm resistance to colonization by Raoiella indica (Acari: Tenuipalpidae)

    Although coconut (Cocos nucifera L.) is the predominant host for Raoiella indica Hirst (Acari: Tenuipalpidae), false spider mite infestations do occur on bananas and plantains (Musa spp. Colla). Since its introduction, the banana and plantain industries have been negatively impacted to different deg...

  2. A mite (Acari: Erythraeidae) as unusual parasite on an adult caddisfly (Trichoptera: Hydroptilidae) from the Dominican Republic (West Indies)

    Botosaneanu, L.; Bolland, H.R.

    1997-01-01

    BOTOSANEANU, L. & H.R. BOLLAND, 1997. A mite (Acari: Erythraeidae) as unusual parasite on an adult caddisfly (Trichoptera: Hydroptilidae) from the Dominican Republic (West Indies). Studies Nat. Hist. Caribbean Region 73, Amsterdam 1997: 71-76. First known case of a caddisfly (adult Leucotrichia tubifex Flint, 1964) parasitized by a mite (larva of Leptus sp.) belonging to another group than Hydrachnellae.

  3. New feather mites of the subfamily Pterodectinae (Acari: Proctophyllodidae) from passerines and woodpeckers (Aves: Passeriformes and Piciformes) in Vietnam

    Mironov, S.; Literák, I.; Hung, N. M.; Čapek, Miroslav

    2012-01-01

    Roč. 3440, č. 3440 (2012), s. 1-49. ISSN 1175-5326 R&D Projects: GA MŠk LC06073 Institutional support: RVO:68081766 Keywords : Acari * feather mites * Proctophyllodidae * systematics * Aves * Passeriformes * Piciformes * Vietnam Subject RIV: EG - Zoology Impact factor: 0.974, year: 2012 http://www.mapress.com/zootaxa/2012/f/zt03440p049.pdf

  4. Ticks (Acari: Ixodida) on wild carnivores in Brazil.

    Labruna, Marcelo B; Jorge, Rodrigo S P; Sana, Dênis A; Jácomo, Anah Tereza A; Kashivakura, Cyntia K; Furtado, Mariana M; Ferro, Claudia; Perez, Samuel A; Silveira, Leandro; Santos, Tarcísio S; Marques, Samuel R; Morato, Ronaldo G; Nava, Alessandra; Adania, Cristina H; Teixeira, Rodrigo H F; Gomes, Albério A B; Conforti, Valéria A; Azevedo, Fernando C C; Prada, Cristiana S; Silva, Jean C R; Batista, Adriana F; Marvulo, Maria Fernanda V; Morato, Rose L G; Alho, Cleber J R; Pinter, Adriano; Ferreira, Patrícia M; Ferreira, Fernado; Barros-Battesti, Darci M

    2005-01-01

    The present study reports field data of ticks infesting wild carnivores captured from July 1998 to September 2004 in Brazil. Additional data were obtained from one tick collection and from previous published data of ticks on carnivores in Brazil. During field work, a total of 3437 ticks were collected from 89 Cerdocyon thous (crab-eating fox), 58 Chrysocyon brachyurus (maned wolf), 30 Puma concolor (puma), 26 Panthera onca (jaguar), 12 Procyon cancrivorus (crab-eating raccoon), 4 Speothos venaticus (bush dog), 6 Pseudalopex vetulus (hoary fox), 6 Nasua nasua (coati), 6 Leopardus pardalis (ocelot), 2 Leopardus tigrinus (oncilla), 1 Leopardus wiedii (margay), 1 Herpailurus yagouaroundi (jaguarundi), 1 Oncifelis colocolo (pampas cat), 1 Eira barbara (tayara), 1 Galictis vittata (grison), 1 Lontra longicaudis (neotropical otter), and 1 Potus flavus (kinkajou). Data obtained from the Acari Collection IBSP included a total of 381 tick specimens collected on 13 C. thous, 8 C. brachyurus, 3 P. concolor, 10 P. onca, 3 P. cancrivorus, 4 N. nasua, 1 L. pardalis, 1 L. wiedii, 4 H. yagouaroundi, 1 Galictis cuja (lesser grison), and 1 L. longicaudis. The only tick-infested carnivore species previously reported in Brazil, for which we do not present any field data are Pseudalopex gymnocercus (pampas fox), Conepatus chinga (Molina's hog-nosed skunk), and Conepatus semistriatus (striped hog-nosed skunk). We report the first tick records in Brazil on two Felidae species (O. colocolo, H. yagouaroundi), two Canidae species (P. vetulus, S. venaticus), one Procyonidae species (P. flavus) and one Mustelidae (E. barbara). Tick infestation remains unreported for 5 of the 26 Carnivora species native in Brazil: Oncifelis geoffroyi (Geoffroy's cat), Atelocynus microtis (short-eared dog), Pteronura brasiliensis (giant otter), Mustela africana (Amazon weasel), and Bassaricyon gabbii (olingo). Our field data comprise 16 tick species represented by the genera Amblyomma (12 species), Ixodes (1

  5. CARACTERIZAÇÃO QUÍMICA E EFEITO DO ÓLEO ESSENCIAL DE ERVA-DE-SANTA-MARIA SOBRE O ÁCARO-RAJADO DE MORANGUEIRO

    JOÃO PAULO PEREIRA PAES; VANDO MIOSSI RONDELLI; ADILSON VIDAL COSTA; ULYSSES RODRIGUES VIANNA; VAGNER TEBALDI DE QUEIROZ

    2015-01-01

    O ácaro-rajado, Tetranychus urticae Koch (1836) (Acari: Tetranychidae), é a principal praga na cultura do morangueiro. O objetivo deste trabalho foi a caracterização química do óleo essencial de Chenopodium ambrosioides L. e a avaliação de seus efeitos sobre o ácaro-rajado. Os compostos do óleo essencial foram identificados pelasanálises de CG-DIC e CG-EM. A mortalidade e a oviposição foram avaliadas após a pulverização do óleo (2,5; 5,0; 7,5 e 10,0% v v-1) sobre fêmeas do ácaro-rajado. O ren...

  6. Reproductive performance of the generalist predator <em>Hypoaspis aculeiferem> (Acari: Gamasida) when foraging on different invertebrate prey

    Heckmann, Lars-Henrik; Ruf, A.; Nienstedt, K. M.;

    2007-01-01

    In this study, we assessed the influence of prey quality and prey biomass during a standardized 3- week test on adult survival and reproductive output of the predatory mite Hypoaspis aculeifer when fed one of six different diets: springtails (Folsomia candida and F. fimetaria), a storage mite (Ca...

  7. Beklemishevia hispaniola n. sp., nuevo representante de la Cohorte Palaeosomata (Acari, Oribatei en España

    Pérez-Iñigo, C.

    1996-12-01

    Full Text Available A new species of oribatid mite (Acari, Oribatei belonging to the family Ctenacaridae (Cohors Palaeosomata is described; this species was recorded several times in Spain as Beklemishevia galeodula Zachvatkin, 1945. The new species is easily distinguishable from the mentioned one because of the presence of three claws in every leg, a noticeable pigidial neotrichy and the absence of short, almost spiniform, setae on the pygidium.Se describe una nueva especie de oribátido (Acari, Oribatei perteneciente a la familia Ctenacaridae (Cohorte Palaeosomata que habia sido citada varias veces en España como Beklemishevia galeodula Zachvatkin, 1945, y de la que se diferencia por presentar tres uñas en todas las patas, una acentuada neotriquia pigidial y carecer de setas cortas espiniformes en el pigidio.

  8. Prolixus (Acari: Trombidiformes: Tenuipalpidae) newly recorded from New Zealand: A new species from Cyperaceae and its ontogenetic patterns in chaetotaxy.

    Xu, Yun; Zhang, Zhi-Qiang

    2014-01-01

    The genus Prolixus (Acari: Tenuipalpidae) was represented by two species from Australian sedges prior to this study. A new species, Prolixus meyerae sp. nov., is here described and illustrated from leaves of Gahnia (Cyperaceae) in Auckland, New Zealand. In this paper, we present the ontogenetic additions in idiosomal and leg chaetotaxy from larva to adult. A key to world species of Prolixus is also proposed. PMID:25543721

  9. Garrapatas (Acari: Ixodidae asociadas a perros en diferentes ambientes de la provincia de Corrientes, Argentina Ticks (Acari: Ixodidae of dogs in different environments of the Corrientes Province, Argentina

    V.N. Debárbora

    2011-06-01

    Full Text Available Se estudiaron las garrapatas (Acari: Ixodidae de perros en ambientes urbanos, periurbanos y rurales de la provincia de Corrientes por medio de colecciones mensuales durante un año en siete sitios diferentes. De los 138 perros examinados, en 87 (63,04% se determinaron tres especies de garrapatas: Amblyomma tigrinum (n=35, Amblyomma ovale (n=2 y Rhipicephalus sanguineus sensu lato (n=523. Las mayores prevalencias para A. tigrinum y R. sanguineus s.l. fueron halladas en ambientes rurales. Especímenes de R. sanguineus s.l. fueron detectados a lo largo de todo el año, con picos de abundancia en primavera y verano, A. tigrinum fue encontrada en otoño, invierno y primavera con un pico en esta última estación, mientras que A. ovale fue colectada sólo en primavera. Rhipicephalus sanguineus s.l. y A. tigrinum fueron halladas en todos los ambientes muestreados, pero el primer taxón fue siempre el más abundante. Teniendo en cuenta que las especies del complejo R. sanguineus son potenciales vectores de microorganismos patógenos para los perros y humanos, y debido a la alta prevalencia registrada en este estudio, queda en evidencia la relevancia de aplicar métodos para su control en las áreas incluidas en este estudio.Ticks (Acari: Ixodidae of dogs in urban, peri-urban and rural areas from the Corrientes Province, Argentina, were studied. They were monthly collected during one year at 7 different sites. A total of 138 dogs were examined, and 87 (63.04% of them were infested; and three species were determined: Amblyomma tigrinum (n=35, Amblyomma ovale (n=2 and Rhipicephalus sanguineus sensu lato (n=523. Both A. tigrinum and R. sanguineus s.l. had the highest prevalence in rural areas. Specimens of R. sanguineus s.l. were collected in the four seasons, but the peaks of abundance were detected in spring and summer. Amblyomma tigrinum was found in autumn, winter and spring, with the peak of abundance in spring, and the two specimens of A. ovale were

  10. First record of the genus .i.Bloszykiella./i. in Kenya with the description of .i.Bloszykiella tertia./i. sp n. (Acari: Uropodidae) from a .i.Pinus radiata./i. D. Don plantation

    Kontschán, J.; Starý, Josef

    2015-01-01

    Roč. 56, č. 3 (2015), s. 629-635. ISSN 1681-5556 Institutional support: RVO:60077344 Keywords : Acari * new species * Pinus radiata plantation * Kenya Subject RIV: EG - Zoology Impact factor: 0.464, year: 2014

  11. Description of a new species of bat-associated argasid tick (Acari: Argasidae) from Brazil.

    Dantas-Torres, Filipe; Venzal, José M; Bernardi, Leopoldo F O; Ferreira, Rodrigo L; Onofrio, Valéria C; Marcili, Arlei; Bermúdez, Sergio E; Ribeiro, Alberto F; Barros-Battesti, Darci M; Labruna, Marcelo B

    2012-02-01

    A new species of argasid tick (Acari: Argasidae) is described from immature and adult specimens collected from several localities in Brazil. A complete morphological account is provided for all postembryonic life stages, i.e., larva, nymph, female, and male. Ornithodoros cavernicolous n. sp. is the 113(th) in the genus. Morphologically, the new species shares common features, e.g., presence of well-developed cheeks and legs with micromammillate cuticle, with other bat-associated argasid ticks included in the subgenus Alectorobius. In particular, the new species is morphologically related to Ornithodoros azteci Matheson, with which it forms a species group. Phylogenetic analysis based on the 16S rRNA gene sequences supports the placement of the new species within a large clade that includes other New World bat-associated argasids. However, the new species seems to represent an independent lineage within the genus Ornithodoros. PMID:21955330

  12. Arthropod colonization of land--linking molecules and fossils in oribatid mites (Acari, Oribatida).

    Schaefer, Ina; Norton, Roy A; Scheu, Stefan; Maraun, Mark

    2010-10-01

    Terrestrial fossils that document the early colonization of land are scarce for >100 my after the Cambrian explosion. This raises the question whether life on land did not exist or just did not fossilize. With a molecular dating technique, we analyzed the origin of terrestrial chelicerate microarthropods (Acari, Oribatida) which have a fossil record since the Middle Devonian that is exceptional among soil animals. Our results suggest that oribatid mites originated in the Precambrian (571+/-37 mya) and that the radiation of basal groups coincides with the gap in the terrestrial fossil record between the Cambrian explosion and the earliest fossilized records of continental ecosystems. Further, they suggest that the colonization of land started via the interstitial, approximately 150 my earlier than the oldest fossils of terrestrial ecosystems. Overall, the results imply that omnivorous and detritivorous arthropods formed a major component in early terrestrial food webs, thereby facilitating the invasion of terrestrial habitats by later colonizers of higher trophic levels. PMID:20420932

  13. Benefits and risks of the subcutaneous immunotherapy with acari extracts in allergic rhinoconjunctivitis and bronchial asthma

    Olimpio Rodríguez-Santos

    2014-12-01

    Full Text Available The records of patients from the Allergology Service in the Previsora Policlinic, Camagüey were revised to evaluate benefits and risks of the subcutaneous immunotherapy (ITSC with extracts of acari. The study was observational, analytic and retrospective of cases and controls in allergic rhinoconjunctivitis and bronchial asthma. A total of 160 subjects, older than 18 years old, were chosen. Eighty out of them had already received ITSC with dose increase during 13 weeks and maintenance with monthly injections during 18 months. A total of 80 patients who only received prevention measures and medications during the crises were paired. Questionnaires were applied for quality of rhinoconjunctivitis life and asthma, about the consumption of medications and the frequency of the crises. The adverse events were measured, as they were local and systemic to the cutaneous tests, to the ITSC and the different pharmacological treatments. There was a significant increase of the punctuation of life quality questionnaires, (p=0.011. The consumption of medications decreased in both the cases and the controls, without significant differences (p=0.083. The frequency of the rhinitis and asthma crises decrease in the group of ITSC (p=0.029. Slight local and systemic reactions were reported in both groups with Odds ratio (OR=2.029 in the ITSC group, with a 95% confidence interval of 1.114–3.967 (p=0.019. The results show that the subcutaneous immunotherapy with acari offers benefits and few risks to patients with allergic rhinoconjunctivitis and asthma.

  14. How does Phytoseiulus persimilis find its prey when foraging within a bean plant?

    Zemek, Rostislav; Nachman, G.; Růžičková, Š.

    Motpellier : Montpellier SupAgro, 2008, s. 390-393. ISBN N. [Congress of the European Association of Acarologists /6./. Montpellier (FR), 21.07.2008-25.07.2008] R&D Projects: GA AV ČR IAA6007303 Institutional research plan: CEZ:AV0Z50070508 Keywords : Acari * Phytoseiidae * Tetranychidae Subject RIV: EH - Ecology, Behaviour

  15. Two new families (Acari: Alicorhagiidae and Platyhelminthes: Prorhynchidae) reported for the Hungarian fauna From leaf litter in the Bükk Mountains

    Pfliegler, W.P.; Bolton, S.J.

    2016-01-01

    Two new members of the Hungarian fauna are reported, both of them were collected in beech forest leaf litter in the Bükk Mountains, North-East Hungary: Alicorhagia fragilis Berlese, 1910 (Arthropoda: Arachnida: Acari: Sarcopti-formes: Endeostigmata: Alicorhagiidae) and Geocentrophora baltica (Kennel, 1883) (Platyhelminthes: Rhabditophora: Trepaxonemata: Amplimatricata: 'Lecithoepitheliata': Prorhynchida: Prorhynchidae). The families Alicorhagiidae and Prorhynchidae both represent...

  16. A review of mites of the genus Neharpyrhynchus Fain (Acari: Harpirhynchidae) - ectoparasites of wild birds, including description of four new species

    Martinů, J.; Dusbábek, František; Literák, I.

    2008-01-01

    Roč. 34, č. 2 (2008), s. 197-209. ISSN 0164-7954 Institutional research plan: CEZ:AV0Z60220518 Keywords : Acari * Neharpyrhynchus * Harpirhynchidae * mites * bird s * Czech Republic Subject RIV: EG - Zoology Impact factor: 0.438, year: 2008

  17. A new species of Aculops (Acari: Prostigmata: Eriophyidae) from Serbia on Dipsacus laciniatus L. (Dipsacaceae), a weed target of classical biological control in the United States of America

    The new eriophyid mite species Aculops dipsaci n. sp. (Acari: Prostigmata: Eriophyidae) collected from Dipsacus laciniatus L. (Dipsacaceae) in northern Serbia is described and illustrated. Differential diagnosis is provided in comparison with Aculops salixis Xue, Song and Hong. This is the first e...

  18. Molecular characterization of a new monopartite dsRNA mycovirus from mycorrhizal Thelephora terrestris (Ehrh.) and its detection in soil oribatid mites (Acari: Oribatida)

    Petrzik, Karel; Sarkisova, Tatiana; Starý, Josef; Koloniuk, Igor; Hrabáková, Lenka; Kubešová, Olga

    2016-01-01

    Roč. 489, FEB 2016 (2016), s. 12-19. ISSN 0042-6822 R&D Projects: GA MŠk LH13136 Institutional support: RVO:60077344 Keywords : Acari * Earthfan fungus * Mycovirus * Oribatida Subject RIV: EE - Microbiology, Virology Impact factor: 3.321, year: 2014

  19. Phytochemical and Biological Studies of <em>Agave em>attenuata>

    Vincenzo De Feo; Muhammad Riaz; Nasir Rasool; Muhammad Zubair; Komal Rizwan; Muhammad Zia-Ul-Haq

    2012-01-01

    The present study was conducted to examine various biological activities of a methanol extract of <em>Agave attenuataem> leaves. GC-MS analysis of the <em>n>-hexane fraction from the extract revealed the presence of 31 compounds, with mono-2-ethylhexyl phthalate (11.37%), 1,2-benzenedicarboxylic acid (6.33%), <em>n>-docosane (6.30%) and eicosane (6.02%) as the major components. The leaves contained appreciable levels of total phenolic contents (10...

  20. Progress in the complete mitochondrial genomes of the Acari%蜱螨线粒体基因组研究进展

    袁明龙; 王进军

    2012-01-01

    Acari, including ticks and mites, is one of the most diverse group of arthropods. In this paper, we reviewed the research progress in the 28 sequenced complete mitochondrial genomes of acari species. These mitochondrial genomes have several marked features; (1) There is significant variation in the size of mitochondrial genomes among the 28 acari species, and the mitochondrial genome of Panonychus citri (13 077 bp) is the smallest among all sequenced arthropods; (2) The base composition of all acari mitochondrial genomes are biased toward A and T, with six species harboring reverse GC-skew values (positive value) ; (3) The base composition and the position, length, copy number of the A +T-rich regions vary greatly among the 28 acari species, of which four tetranychid species harbor the highest A +T content within acari and the shortest A +T-rich region (44-57 bp) among arthropods; (4) High gene rearrangements are found in acari mitochondrial genomes, especially in those of Acariformes, but the rearrangements are not correlated to high taxonomic ranks; (5) The tRNA genes in some species of Acariformes are extremely truncated, presenting atypical cloverleaf structures. We suggest that it is necessary to sequence more acari mitochondrial genomes aiming to investigate whether these tRNA genes lacking both D- and T-arms are functional or not, to analyze the molecular mechanisms of evolution in acari mitochondrial genomes, and to carry out the acari mitochondrial transcriptome studies.%蜱螨亚纲包括蜱类和螨类,是节肢动物中物种多样性最高的类群之一.本文综述了当前已测序的28种蜱螨线粒体基因组的研究成果.概括起来,蜱螨线粒体基因组具有以下特点:(1)大小变异显著,其中柑橘全爪螨Panonychus citri线粒体基因组在目前已测节肢动物中最小(13077 bp);(2)一般碱基组成偏向A和T,但6种蜱螨具有相反的GC-偏斜(正值);(3)基因组的碱基组成及A+T富集区的位置、长度和

  1. Application of gamma radiation on longevity of some mites species (Acari: Tetranychidade)

    Machi, Andre R., E-mail: rica_machi@hotmail.com [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Arthur, Valter, E-mail: arthur@cena.usp.br [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)

    2015-07-01

    Mites are pests agricultural found in various environments accessible to animal life: soil, aerial parts of the plants, host insects. In this research the effects of gamma irradiation on longevity of mite pests of the tetranychidae family have been studied. The mites were irradiated in a source of Cobalt-60, Gammacell-220 type, at a dose rate of 0.486 kGy located in the CENA/USP, in the doses of 0 (control), 100, 200, 300, and 400 Gy with sixteen replicates per dose. After the irradiation, the mites were placed in petri dishes totalizing 5 treatments in 32 repetitions. The analysis of variance design with completely randomized design using the Statistical Analysis System (SAS) and the Tukey test, the verification of means. Were evaluated daily the adult mortality and longevity of irradiated mites. After 25 days was observed a mean longevity of mites, for O.ilicis, 100 Gy was equal the control dose (18.3 days), but to T. desertorum and T. urticae the larger longevity was observed in the dose of 200 Gy (19.0 days) being that this dose, obtained the larger longevity in comparison to control dose (18.5 days), in general the longevity decreased in relation to increased doses. Thus, only the dose of 100 Gy and 200 Gy stimulated an increased the longevity in O. ilicis and T. desertorum and T. urticae respectively. The exact mechanism by which the mites are tolerant to avoid damage caused by radicals when exposed to ionizing radiation is not fully understood. (author)

  2. Application of gamma radiation on longevity of some mites species (Acari: Tetranychidade)

    Mites are pests agricultural found in various environments accessible to animal life: soil, aerial parts of the plants, host insects. In this research the effects of gamma irradiation on longevity of mite pests of the tetranychidae family have been studied. The mites were irradiated in a source of Cobalt-60, Gammacell-220 type, at a dose rate of 0.486 kGy located in the CENA/USP, in the doses of 0 (control), 100, 200, 300, and 400 Gy with sixteen replicates per dose. After the irradiation, the mites were placed in petri dishes totalizing 5 treatments in 32 repetitions. The analysis of variance design with completely randomized design using the Statistical Analysis System (SAS) and the Tukey test, the verification of means. Were evaluated daily the adult mortality and longevity of irradiated mites. After 25 days was observed a mean longevity of mites, for O.ilicis, 100 Gy was equal the control dose (18.3 days), but to T. desertorum and T. urticae the larger longevity was observed in the dose of 200 Gy (19.0 days) being that this dose, obtained the larger longevity in comparison to control dose (18.5 days), in general the longevity decreased in relation to increased doses. Thus, only the dose of 100 Gy and 200 Gy stimulated an increased the longevity in O. ilicis and T. desertorum and T. urticae respectively. The exact mechanism by which the mites are tolerant to avoid damage caused by radicals when exposed to ionizing radiation is not fully understood. (author)

  3. Chlorotic spots on Clerodendrum, a disease caused by a nuclear type of Brevipalpus (Acari:Tenuipalpidae transmitted virus Mancha clorótica do Clerodendrum, uma enfermidade causada por um vírus do tipo nuclear, transmitido pelo ácaro Brevipalpus phoenicis (Acari:Tenuipalpidae

    Elliot Watanabe Kitajima

    2008-02-01

    Full Text Available Chlorotic spots have been observed in plants of Clerodendrum x speciosum growing in residential gardens and parks in Piracicaba, SP, Brazil. Thin sections of diseased tissues revealed characteristic cytopathic effects of the nuclear type of the Brevipalpus (Acari: Tenuipalpidae mite-transmitted viruses (BTrV. Brevipalpus mites, identified as B. phoenicis, infesting symptomatic C. x speciosum plants transmitted the pathogen to healthy C. x speciosum and to C. thomsonae, Gomphrena globosa, Hibiscus cannabinus, H. coccineus, H. schizopetalus, Salvia leucantha, Spathiphyllum wallasi and Tetragonia expansa causing chlorotic spots on their leaves. Mechanical inoculation using leaf extracts from infected C. x speciosum resulted in chlorotic spots on inoculated C. x speciosum, Chenopodium quinoa, C. amaranticolor, G. globosa, H. cannabinus, H. coccineus and T. expansa leaves. C. amaranticolor and C. quinoa kept at 28 - 30°C became systemically infected. The same cytopathic effects caused by the nuclear type of BTrV were seen in tissues from all infected test plants by electron microscopy. The virus was purified from systemically infected leaves of C. amaranticolor and C. quinoa. A polyclonal antiserum obtained from an immunized rabbit presented a strong reaction with the homologous antigen in ELISA tests. The results suggest that this chlorotic spot disease of C. x speciosum is caused by a new species of the nuclear type of BTrV, tentatively named Clerodendrum chlorotic spot virus (ClCSV.Manchas cloróticas e necróticas foram observadas em folhas de várias plantas de coração-sangrento (Clerodendrum x speciosum cultivadas em parques e jardins em Piracicaba, SP, associadas à infestação pelo ácaro tenuipalpídeo Brevipalpus phoenicis. Exames preliminares de secções de tecido das manchas cloróticas ao microscópio eletrônico revelaram a ocorrência de efeitos citopáticos característicos dos induzidos pelos vírus do tipo nuclear, transmitido

  4. A new species of Litarachna (Acari, Hydrachnidia, Pontarachnidae from a Caribbean mesophotic coral ecosystem

    Vladimir Pesic

    2014-07-01

    Full Text Available New records of pontarachnid mites (Acari: Hydrachnidia from the Caribbean island of Puerto Rico are presented. Litarachna lopezae sp. n., from substrata collected from Bajo de Sico, a mesophotic coral reef ecosystem in Mona Passage off Puerto Rico, is described as new to science. The new species was collected from nearly 70 m depth, the greatest depth from which pontarachnid mites have been found until now. In addition, a Litarachna sp. was also found in association with the tube of the polychaete Sabellastarte magnifica (Shaw, 1800 at the shallow waters of north Puerto Rico.

  5. Diversidade e dinâmica populacional de ácaros em pomar cítrico

    Marcos Zatti da Silva

    2012-01-01

    Full Text Available As plantas de citros hospedam diversas espécies de ácaros que causam sérios prejuízos à produção e qualidade de frutos no Brasil. Esta pesquisa teve por objetivo estudar a diversidade de ácaros e a dinâmica populacional das principais espécies de ácaros em pomar de citros, Citrus sinensis (L. Osbeck, localizado no município de Descalvado, Estado de São Paulo. Foram realizadas 14 avaliações em um período de 12 meses, de fevereiro de 2007 a fevereiro de 2008. Em cada avaliação, foram coletadas dez folhas e cinco frutos na parte interna de cada uma das 40 plantas demarcadas no pomar. Foram encontradas 34 espécies de ácaros, distribuídas em 17 famílias. As espécies mais abundantes foram: Phyllocoptruta oleivora (Ashmed (Eriophyidae, Panonychus citri (McGregor (Tetranychidae, Brevipalpus phoenicis (Geijskes (Tenuipalpidae e Fungitarsonemus sp. (Tarsonemidae. Entre os ácaros predadores, a família Phytoseiidae foi a de maior riqueza (oito espécies e abundância (1.072 indivíduos. A espécie predominante foi Euseius concordis (Chant, correspondendo a 98,3% dos ácaros desta família. Foram observadas correlações significativas entre densidades populacionais de E. concordis e dos ácaros fitófagos P. citri, B. phoenicis e P. oleivora. Também foram observadas correlações significativas entre densidades populacionais desses ácaros e fatores meteorológicos como temperatura e precipitação pluvial. Os resultados indicam que E. concordis atua como inimigo natural de P. citri, B. phoenicis e P. oleivora no pomar cítrico estudado.

  6. Thermal requirements of Dermanyssus gallinae (De Geer, 1778 (Acari: Dermanyssidae

    Edna Clara Tucci

    2008-06-01

    Full Text Available The thermal requirements for development of Dermanyssus gallinae were studied under laboratory conditions at 15, 20, 25, 30 and 35°C, a 12h photoperiod and 60-85% RH. The thermal requirements for D. gallinae were as follows. Preoviposition: base temperature 3.4ºC, thermal constant (k 562.85 degree-hours, determination coefficient (R² 0.59, regression equation: Y= -0.006035 + 0.001777 x. Egg: base temperature 10.60ºC, thermal constant (k 689.65 degree-hours, determination coefficient (R² 0.94, regression equation: Y= -0.015367 + 0.001450 x. Larva: base temperature 9.82ºC, thermal constant (k 464.91 degree-hours, determination coefficient R² 0.87, regression equation: Y= -0.021123+0.002151 x. Protonymph: base temperature 10.17ºC, thermal constant (k 504.49 degree-hours, determination coefficient (R² 0.90, regression equation: Y= -0.020152 + 0.001982 x. Deutonymph: base temperature 11.80ºC, thermal constant (k 501.11 degree-hours, determination coefficient (R² 0.99, regression equation: Y= -0.023555 + 0.001996 x. The results obtained showed that 15 to 42 generations of Dermanyssus gallinae may occur during the year in the State of São Paulo, as estimated based on isotherm charts. Dermanyssus gallinae may develop continually in the State of São Paulo, with a population decrease in the winter. There were differences between the developmental stages of D. gallinae in relation to thermal requirements.Experimentos de laboratório foram realizados visando estimar as exigências térmicas de Dermanyssus gallinae. Para isso, o desenvolvimento do ácaro foi estudado em condições de laboratório usando-se câmaras climatizadas reguladas a 15, 20, 25, 30 e 35°C, fotofase de 12h e UR de 6085%. As exigências térmicas determinadas para D. gallinae foram: Pré-oviposição: temperatura base de 3,4 ºC, constante térmica (k igual a 562,85 graus-hora, coeficiente de determinação (R² igual a 0,59, equação de regressão: Y= -0,006035 + 0

  7. A revision of the Pheroliodidae, fam. n. (Acari: Oribatei

    Adilson D. Paschoal

    1986-01-01

    Full Text Available The new family Pheroliodidae, herei nproposed, includes the following genera and species Pheroliodes Grandjean (in whose synonymy Pedrocortesia Hammer is placed, with the species: casa-branquensis, sp. n. (Brazil, São Paulo, Casa Branca, pellitus, sp. n. (Brazil, São Paulo, Piracicaba, nemoricultricis, sp. n. (Brazil, São Paulo, Piracicaba, wehnekei (Willmann (Guatemala, Venezuela, roblensis Covarrubias (Chile, mirabilis (Hammer, n. comb. (Argentina: Pedrocortesia elegans Hammer, P. intermedia Hammer, both from Peru, P. fissurata Balogh & Mahunka and P. inaequalis Balogh & Mahunka, both from Mongolia, P. franzi Balogh (Chad, P. africana Balogh (Kenya, P. vermicularis Balogh (New Guinea and P. sculptrata Aoki (Corea are considered incertae sedis; Lopholiodes, gen. n., includes the species micropunctinatum, sp. n., the type-species (Brazil, São Paulo, Anhumas and macropunctinatum, sp. n. (Brazil, São Paulo, Piracicaba: Octoliodes, gen n., includes the species leuteomarginatus (Hammer, n. comb., the type-species (New ealand and rotoruensis (Hammer, n. comb. (New Zealand: and Licnoliodes Grandjean, with the species: andrei Grandjean, type-species (Spain and Algeria, adminensis Grandjean, type-species (Spain and Algeria, adminensis Grandjean (Maroc, Algeria, Spain and apunctatus Mahunka (Greece.A nova família aqui descrita, Pheroliodidae, inclui os seguintes gêneros e espécies: Pheroliodes Grandjean (em cuja sinonímia vai incluído Pedrocortesia Hammer, com as seguintes espécies: casa-branquensis, sp. n. (Brasil, São Paulo, Casa Branca, pellitus, sp. n. (Brasil, São Paulo, Piracicaba, nemoricultricis, sp. n. (Brasil, São Paulo, Piracicaba, wehnekei (Willmann (Guatemala, Venezuela, roblensis Covarrubias (Chile, mirabilis (Hammer, n. comb. (Argentina: Pedrocortesia elegans Hammer, P. intermedia Hammer, ambas do Peru, P. fissurata Balogh & Mahunka (Mongólia, inaequalis Balogh & Mahunka (Mongólia, franzi Balogh (Chad, P. africana Balogh

  8. Anatomia de lesões foliares causadas pelo vírus da Mancha Clorótica do Clerodendrum, transmitido pelo ácaro Brevipalpus phoenicis em diferentes espécies

    Renata Takassugui Gomes; Elliot Watanabe Kitajima; Francisco André Osamu Tanaka; João Paulo Rodrigues Marques; Beatriz Appezzato-da-Glória

    2010-01-01

    O gênero botânico Clerodendrum pertence à família Lamiaceae e compreende várias espécies ornamentais, Manchas cloróticas e necróticas em folhas de coração-sangrento foram observadas pela primeira vez em um jardim de Piracicaba, SP, associadas à infestação com Brevipalpus phoenicis (Acari: Tenuipalpidae). Exames de secções de tecidos das lesões foliares ao microscópio eletrônico revelaram ocorrência de efeitos citopáticos do tipo nuclear e concluiu-se que os sintomas eram causados por um vírus...

  9. Acaricidal and repellent activities of essential oil of Eucalyptus globulus against Dermanyssus gallinae (Acari: Mesostigmata

    Dehghani-Samani Amir

    2015-07-01

    Full Text Available Introduction: By considering an increase in drug resistance against red mites, finding the nonchemical herbal acaricide against Dermanyssus gallinae (De Geer (Acari: Mesostigmata is necessary to kill them and to reduce the chemical resistance against chemical acaricides in this specie. Dermanyssus gallinae is a potential vector of the causal agent of several viral diseases such as Equine encephalitis and St. Louis encephalitis. It can be a vector of bacteria such as Salmonella spp., Mycobacterium spp. and Erysipelothrix rhusiopathiae. It is also known to cause itching dermatosis in humans. In this study acaricidal and repellent activities of essential oil of Eucalyptus globulus against Dermanyssus gallinae were studied. Methods: After extracting the essential oil, different concentrations of the plant extract were prepared. Then, acaricidal effect of different concentrations was tested on poultry red mite, Dermanyssus gallinae, by dropping 3-4 drops of essential oil on mites. Repellent activity of essential oil was tested by Y-tube olfactometer bioassay. After the test, total number of killed and repellent mites reported. Results: Concentration of 1:2 or 50% had more acaricidal effect on mites. Also essential oil of Eucalyptus globulus had repellent activity against red mites. Conclusion: This study showed that essential oil of Eucalyptus globulus had acaricidal and repellent activities against red mites. Hence it might be used as a herbal acaricide against it to kill and to reduce the chemical resistance in this specie.

  10. Historical review of the genus Dermanyssus Dugès, 1834 (Acari: Mesostigmata: Dermanyssidae

    Roy L.

    2007-06-01

    Full Text Available A synthetic review of the historical systematics of Dermanyssus Dugès, 1834 (Acari: Mesostigmata: Dermanyssidae is provided. The classification at the specific level in this early genus has not really been clarified during more than a century despite its economic impact, and the history of the genus is complex and includes various stages. Moreover, Dermanyssus currently includes 23 species, whereas the last review took only 18 species into account. Changes in the species status and position in the genus Dermanyssus from 1834 until today are presented. The evolution of the generic definition is explored and compared with other genera of the group. How the discrimination between the different species evolved in the genus is also examined. Some difficulties in the specific definitions are discussed. A current diagnosis of the genus Dermanyssus is given. A table of the species included in this genus since its first description along with their respective current positions, a list of the currently included species in Dermanyssus with their hosts, and a world map presenting their geographic distribution are provided.

  11. Distribution of Chaetodactylus krombeini (Acari: Chaetodactylidae) within Osmia cornifrons (Hymenoptera: Megachilidae) nests: implications for population management.

    McKinney, Matthew I; Park, Yong-Lak

    2013-06-01

    Chaetodactylus krombeini (Baker) (Acari: Chaetodactylidae) is a cleptoparasitic mite that negatively affects propagation of Osmia spp. (Hymenoptera: Megachilidae) for orchard pollination in the USA. This study was conducted to determine the effect of C. krombeini on mortality of male and female Osmia cornifrons, the Japanese hornfaced bee. A total of 107 O. cornifrons nests were examined to determine within-nest distribution of C. krombeini with regression analyses. A total of 30 mite-free O. cornifrons nests were observed and within-nest distribution of male and female O. cornifrons was determined with non-linear regression analyses. In addition, cocoons from 20 mite-infested O. cornifrons cells were examined to determine whether C. krombeini could be found inside cocoons of O. cornifrons. The results of this study showed that female O. cornifrons and C. krombeini were found more frequently in the inner part of the nest, and male O. cornifrons were found mostly in the center of the nest. No C. krombeini were found inside O. cornifrons cocoons. These results indicate that C. krombeini have a greater negative impact on mortality in the egg and larval stages of female O. cornifrons than in male O. cornifrons. Implications for management of C. krombeini and O. cornifrons populations for orchard pollination are discussed in this article. PMID:23100109

  12. Flagging versus dragging as sampling methods for nymphal Ixodes scapularis (Acari: Ixodidae)

    Rulison, Eric L.; Kuczaj, Isis; Pang, Genevieve; Hickling, Graham J.; Tsao, Jean I.; Ginsberg, Howard S.

    2013-01-01

    The nymphal stage of the blacklegged tick, Ixodes scapularis (Acari: Ixodidae), is responsible for most transmission of Borrelia burgdorferi, the etiologic agent of Lyme disease, to humans in North America. From 2010 to fall of 2012, we compared two commonly used techniques, flagging and dragging, as sampling methods for nymphal I. scapularis at three sites, each with multiple sampling arrays (grids), in the eastern and central United States. Flagging and dragging collected comparable numbers of nymphs, with no consistent differences between methods. Dragging collected more nymphs than flagging in some samples, but these differences were not consistent among sites or sampling years. The ratio of nymphs collected by flagging vs dragging was not significantly related to shrub density, so habitat type did not have a strong effect on the relative efficacy of these methods. Therefore, although dragging collected more ticks in a few cases, the numbers collected by each method were so variable that neither technique had a clear advantage for sampling nymphal I. scapularis.

  13. Spatial distribution of galls caused by Aculus tetanothrix (Acari: Eriophyoidea) on arctic willows.

    Kuczyński, Lechosław; Skoracka, Anna

    2005-01-01

    The distribution of galls caused by Aculus tetanothrix (Acari: Eriophyoidea) on three Salix species was studied. The factors influencing this distribution were analysed, i.e. willow species, study area and shoot length. Spatial pattern of gall distribution within the shoot was also examined. The study was conducted in Russia, Kola Peninsula. Densities of galls caused by A. tetanothrix differed significantly among willow species. Considerably higher gall density was recorded in the White Sea coast than in the Khibiny Mountains. This may be explained by the influence of a milder maritime climate that favors mite occurrence compared to a harsh and variable mountain climate that limits mite abundance. There was no relationship between the gall density and the shoot length. The highest density of galls was recorded on the inner offshoots; within the offshoot, there was a maximum density on the fifth leaf. This pattern was repeatable for all shoots studied, independent of the study area, willow species and length of shoots, suggesting the optimal conditions for A. tetanothrix exist on leaves in the middle part of a shoot. This distribution pattern may be an effect of the trade-off between the costs and benefits resulting from leaf quality and mite movement along the shoot. This hypothesis, however, needs to be tested experimentally. PMID:16132741

  14. Ticks (Acari: Ixodidae) infesting birds in an Atlantic rain forest region of Brazil.

    Ogrzewalska, Maria; Pacheco, Richard C; Uezu, Alexandre; Richtzenhain, Leonardo J; Ferreira, Fernando; Labruna, Marcelo B

    2009-09-01

    Brazil has the third richest bird diversity of the world; however, there are few data concerning ticks (Acari: Ixodidae) parazitizing birds. The aim of the study was to report tick infestations on wild birds from an Atlantic rain forest region of Brazil. During 2 yr, ticks were collected from birds and from the environment in 12 forest sites. A total of 1,725 birds were captured representing 80 species from 24 families. In total, 223 (13%) birds were found infested by immature stages of Amblyomma ticks: 1,800 larvae and 539 nymphs. The prevalence of ticks was higher among birds from the families Thamnophilidae, Conopophagidae, and Momotidae. The most common tick parasitizing birds was Amblyomma nodosum Koch. Other tick species, Amblyomma coelebs Neumann, Amblyomma cajennense (F.), Amblyomma ovale Koch, Amblyomma longirostre (Koch), Amblyomma calcaratum Neumann, and Amblyomma naponense (Packard), were found sporadically. Among free-living ticks collected in the environment, A. cajennense was the most common, followed by A. coelebs, A. naponense, Amblyomma brasilense Aragão, and Hemaphysalis juxtakochi Cooley. PMID:19769058

  15. Secondary structure of expansion segment D1 in LSU rDNA from Arachnida and its phylogenetic application in Eriophyoid mites and in Acari.

    Wang, Zheng-Hang; Zhao, Ya-E; Xu, Yang; Hu, Li; Chen, Yi-Meng

    2015-12-01

    An increasing number of researchers have applied secondary-structure based multiple alignments of rDNA genes in phylogeny. These studies mostly depended on a few valuable divergent domains in LSU and SSU rDNA. Yet other divergent domains, e.g. D1, were poorly investigated and rarely used. However, these domains might contain additional evolutionary data and play a vital role in DNA-based phylogenetic study. Here, we investigated all available D1 sequences of Arachnida taxa and predicted corresponding secondary structures to help identify homologous positions in the D1 region. Long insertions were found exclusive to Eriophyoidea and folded into three newly proposed helices. Non-Acari taxa were all GC rich. In Acari, most Trombidiformes and all Mesostigmata (Parasitiformes) taxa were AT rich and Ixodida (Parasitiformes) GC rich; however there was no consistent base bias in Sarcoptiformes sequences. For Eriophyoid mites, genera Cecidophyopsis and Aceria were both well supported in MP, NJ, ME and ML tress based on D1 sequences, and clusters of Cecidophyopsis species were identical with former study. This demonstrated that the D1 region could act as a valuable molecular marker in phylogenetic reconstruction of Eriophyoidea. Additionally, D1 has been proven suitable in phylogenetic analysis at the family and genus level in Acari, but not in Opiliones. PMID:26420464

  16. Canis familiaris, UN NUEVO HOSPEDERO DE Ornithodoros (A. puertoricensis FOX, 1947 (ACARI: IXODIDA EN COLOMBIA

    BEJARANO EDUAR E. E.

    2009-04-01

    Full Text Available

    RESUMEN

    Las garrapatas revisten gran importancia en el campo biomédico por sus hábitos hematófagos y asociación con la transmisión de agentes patógenos a humanos y animales. El objetivo de esta investigación fue establecer las especies de garrapatas que parasitan perros en tres poblaciones del área rural del Caribe colombiano. Durante los meses de agosto y diciembre del año 2006 se realizó búsqueda activa de garrapatas sobre caninos domésticos de las localidades de El Campín, Sabanas del Potrero y Escobar Arriba, departamento de Sucre. Las garrapatas recolectadas fueron almacenadas en viales con etanol al 70% e identificadas empleando claves morfológicas de referencia para cada familia. Para la determinación de especie en la familia Argasidae se realizaron estimaciones morfométricas de estructuras externas. Se recolectaron 420 garrapatas a partir de 50 caninos infestados, de un total de 134 perros examinados, que corresponde a una tasa de infestación del 37,3%. Las garrapatas fueron identificadas como <em>Rhipicephalus sanguineusem>, <em>Rhipicephalus em>(Boophilus> <em>microplus em>y <em>Amblyomma ovale em>pertenecientes a la familia Ixodidae, y <em>Ornithodoros em>(Alectorobius> <em>puertoricensis em>de la familia Argasidae. La especie predominante fue <em>R. sanguineus em>(92,1% en los estados de larva, ninfa y adulto, seguida por larvas de <em>O. puertoricensisem>, que fueron halladas en menor número sobre caninos de las tres localidades. Se registra, por primera vez en América, el parasitismo de <em>O. puertoricensis em>sobre caninos domésticos y se confirma su presencia en Colombia.

    Palabras clave

  17. Description of a new argasid tick (Acari: Ixodida) from bat caves in Brazilian Amazon.

    Nava, Santiago; Venzal, Jose M; Terassini, Flavio A; Mangold, Atilio J; Camargo, Luis Marcelo A; Labruna, Marcelo B

    2010-12-01

    Nothoaspis amazoniensis n. sp. (Acari: Ixodida: Argasidae) is described from adult and immature ticks (nymph II, nymph I, larva) collected from bat caves in the Brazilian Amazon. Also, 16S rDNA sequences are provided. The diagnostic characters for adults are the presence of false shield or nothoaspis, an anteriorly projecting hood covering the capitulum, a medial extension of palpal article I (flaps), genital plate extending from coxa I to IV, absence of 2 setae on the internal margin of the flaps, a minute hypostome without denticles, presence of a central pore in the base of hypostome, and a reticulate surface pattern on the posterior half of the nothoaspis in males. The nymph II stage is characterized by a hood that is small in relation to the capitulum, short coxal setae, palpal flaps lacking setae on the internal margin, long hypostome, pointed with dentition 4/4 apically, and the anterior half of the body is covered by a cell-like configuration. Nymph I stage is characterized by a hood, small in relation to the capitulum, dorsum of the body covered by a cell-like configuration, venter integument covered by a cell-like configuration, and hypostome dentition 4/4 with apices that are "V"-shaped. Diagnostic characters of the larvae are the number and size of dorsal setae, and the shape of scutum and hypostome. The new species appears to have a life cycle with a larva that feeds on bats, a non-feeding nymphal stage (nymph I), a feeding nymphal stage (nymph II), and adults that probably represent non-feeding stages. PMID:21158616

  18. TAMBO VIEJO: UN ASENTAMIENTO FORTIFICADO EN EL VALLE DE ACARÍ, PERÚ (Tambo Viejo: A Fortified Settlement in the Acari Valley, Peru

    Lidio M. Valdez

    2013-10-01

    Full Text Available La aparición de los asentamientos fortificados y el origen del conflicto violento son temas poco discutidos en el contexto de la arqueología peruana. Considero que es oportuno investigar y determinar cuándo y por qué surgieron los primeros asentamientos fortificados. El propósito central de este artículo está precisamente orientado a responder tales interrogantes y discutir el tema del conflicto violento en el valle de Acarí y, por extensión, en la costa sur del Perú. Las evidencias arqueológicas disponibles señalan que, durante el periodo Intermedio Temprano, Tambo Viejo fue un asentamiento defendido por varias estructuras perimétricas. Otros sitios contemporáneos de Tambo Viejo en Acarí también fueron fortificados. Además, existe en el mismo valle evidencia tangible de violencia en la forma de prisioneros que posteriormente fueron decapitados. En contraste a la evidencia proveniente de Acarí, no existen asentamientos del periodo Intermedio Temprano identificables como fortificaciones, lo que hace de los sitios de Acarí los primeros asentamientos fortificados de toda la costa sur. ENGLISH: The emergence of violent conflict and of fortified settlements is a subject little studied within Peruvian archaeology. However, I consider it vital to investigate and determine the time and the reasons under which fortified settlements were first established. The central aim of this paper is to discuss when and why fortified settlements emerged first in the Acari Valley and, by extension, in the south coast of Peru. Available archaeological evidence indicates that during the Early Intermediate Period, Tambo Viejo was a fortified settlement protected by several massive walls. Other neighbouring sites in Acari were also fortified. Furthermore, in Acari there is conclusive evidence for violence in the form of decapitated individuals. In contrast to evidence coming from Acari, not a single Early Intermediate Period settlement from other

  19. A new species of Tenuipalpus sensu stricto (Acari: Tenuipalpidae) from Brazil, with ontogeny and a key to the known species.

    Castro, Elizeu B; Feres, Reinaldo J F; Ochoa, Ronald; Bauchan, Gary R

    2016-01-01

    The Cerrado is the second largest Brazilian biome, and is considered to be a "hotspot" due the great concentration of endemic species and high rate of deforestation. Surveys of the mite fauna present in this biome have revealed a great number of new species. In this paper, we describe Tenuipalpus spinosaurus sp. nov. (Acari: Tenuipalpidae), a new species of Tenuipalpus sensu stricto, from adult females, deutonymphs, protonymphs, larvae and eggs, collected on Terminalia argentea (Combretaceae), from the Cerrado in Brazil. Females of this new species bear a prominent longitudinal crest on the opisthosoma. The ontogenetic changes in the idiosoma and leg chaetotaxy of all stages are presented. A key to the world species of Tenuipalpus sensu stricto is provided. PMID:27394345

  20. Demodex castoris sp. nov. (Acari: Demodecidae) parasitizing Castor fiber (Rodentia), and other parasitic arthropods associated with Castor spp.

    Izdebska, Joanna N; Fryderyk, Sławomira; Rolbiecki, Leszek

    2016-02-11

    A new species of demodecid mite, Demodex castoris sp. nov. (Acari: Prostigmata: Demodecidae), is described based on adult stages from the skin of the nasal region of the Eurasian beaver Castor fiber Linnaeus, 1758, collected in Poland. This is the first detection of a representative demodecid mite in rodents of the suborder Castorimorpha and also represents the first detection of a skin mite in Eurasian beavers. The new species is a small skin mite (average 173 µm in length) characterized by sexual dimorphism related to body proportions. D. castoris sp. nov. was observed in 4 out of 6 beavers examined (66.6%), with a mean intensity of 10.8 and an intensity range of 2-23 ind. host(-1). This paper also contains a checklist of parasitic arthropods known from Castor spp. PMID:26865230

  1. Reference Gene Selection in the Desert Plant <em>Eremosparton songoricuem>m>

    Dao-Yuan Zhang

    2012-06-01

    Full Text Available <em>Eremosparton songoricum em>(Litv. Vass. (<em>E. songoricumem> is a rare and extremely drought-tolerant desert plant that holds promise as a model organism for the identification of genes associated with water deficit stress. Here, we cloned and evaluated the expression of eight candidate reference genes using quantitative real-time reverse transcriptase polymerase chain reactions. The expression of these candidate reference genes was analyzed in a diverse set of 20 samples including various <em>E. songoricumem> plant tissues exposed to multiple environmental stresses. GeNorm analysis indicated that expression stability varied between the reference genes in the different experimental conditions, but the two most stable reference genes were sufficient for normalization in most conditions.<em> EsEFem> and <em>Esα-TUB> were sufficient for various stress conditions, <em>EsEF> and <em>EsACT> were suitable for samples of differing germination stages, and <em>EsGAPDH>and <em>Es>UBQ em>were most stable across multiple adult tissue samples. The <em>Es18Sem> gene was unsuitable as a reference gene in our analysis. In addition, the expression level of the drought-stress related transcription factor <em>EsDREB2em>> em>verified the utility of<em> E. songoricumem> reference genes and indicated that no single gene was adequate for normalization on its own. This is the first systematic report on the selection of reference genes in <em>E. songoricumem>, and these data will facilitate future work on gene expression in this species.

  2. Synthesis, Crystal Structure and Luminescent Property of Cd (II Complex with <em>N-Benzenesulphonyl-L>-leucine

    Xishi Tai

    2012-09-01

    Full Text Available A new trinuclear Cd (II complex [Cd3(L6(2,2-bipyridine3] [L =<em> Nem>-phenylsulfonyl-L>-leucinato] has been synthesized and characterized by elemental analysis, IR and X-ray single crystal diffraction analysis. The results show that the complex belongs to the orthorhombic, space group<em> Pem>212121 with<em> aem> = 16.877(3 Å, <em>b> em>= 22.875(5 Å, <em>c em>= 29.495(6 Å, <em>α> em>= <emem>= <emem>= 90°, <em>V> em>= 11387(4 Å3, <em>Z> em>= 4, <em>Dc>= 1.416 μg·m−3, <emem>= 0.737 mm−1, <em>F> em>(000 = 4992, and final <em>R>1 = 0.0390, <em>ωR>2 = 0.0989. The complex comprises two seven-coordinated Cd (II atoms, with a N2O5 distorted pengonal bipyramidal coordination environment and a six-coordinated Cd (II atom, with a N2O4 distorted octahedral coordination environment. The molecules form one dimensional chain structure by the interaction of bridged carboxylato groups, hydrogen bonds and p-p interaction of 2,2-bipyridine. The luminescent properties of the Cd (II complex and <em>N-Benzenesulphonyl-L>-leucine in solid and in CH3OH solution also have been investigated.

  3. Selection of resistance to amitraz and analysis of expression difference of cytochrome P450 genes in Panonychus citri (Acari: Tetranychidae)%橘全爪螨对双甲脒的抗性选育及其P450基因的表达差异分析

    冉春; 江高飞; 刘斌; 刘浩强; 李鸿筠; 王进军

    2012-01-01

    To evaluate the resistance risk of Panonychus citri to amitraz and clarify the relationship between expression level of cytochrome P450 gene and resistance of P. citri, resistance selection was conducted in the laboratory, and cross-resistance of the amitraz-resistant strain was studied with slide-dip method and cytochrome P450 gene expression profiles between the resistant strain and the susceptible strain were compared using RPKM (reads per kb per million reads) method. After selection with amitraz continuously for 12 generations, a resistant strain was obtained with the resistance ratio (R/S) of 26. 32 compared with the susceptible strain. The realized heritability of resistance to amitraz was 0. 148. Bioassay data showed that the amitraz-resistant strain had positive cross-resistance to spirodiclofen, diafenthiuron, propargite and azocyclotin (the R/S ratio was 16. 85, 4. 98, 2. 13, and 2. 05, respectively), but had no cross-resistance to abamectin, fenbutatin oxide, pyridaben and petroleum oil (the R/S ratio was 1. 10, 1. 21, 0. 67 and 0. 99, respectively). Gene expression difference analysis results indicated that 16 cytochrome P450 genes were up-regulated and 27 cytochrome P450 genes were down-regulated in the resistant strain. CYP389A6 [ log2ratio ( RS/SS) =11.526] and CYP389A2 [ log2 ratio(RS/SS) = -12.683] were the top up-regulated and down-regulated genes, respectively, which are very likely to be associated with the resistance of P. citri to amitraz.%为了对双甲脒进行抗性风险评估,弄清P450基因在橘全爪螨Panonychus citri抗药性中的作用,在室内用双甲脒对橘全爪螨进行了抗性选育和交互抗性研究,同时分析了橘全爪螨双甲脒抗性和敏感品系P450基因表达差异.经过12代抗性选育,获得了橘全爪螨双甲脒抗性品系,与敏感品系比较,橘全爪螨对双甲脒的抗性倍数达到26.32倍.抗性风险评估表明,橘全爪螨对双甲脒抗性遗传力h2为0.148.螺螨酯、丁醚脲、炔螨特和三唑锡对抗性品系的LC50分别为敏感品系的16.85,4.98,2.13和2.05倍,表明双甲脒抗性品系对螺螨酯、丁醚脲、炔螨特和三唑锡具有明显的交互抗性.阿维菌素、苯丁锡、哒螨灵、矿物油对抗性品系LC50分别为敏感品系的1.10,1.21,0.67和0.99倍,表明双甲脒抗性品系对上述4种药剂没有显著的交互抗性.基因差异性分析发现,抗性品系中有16条P450基因发生了上调,27条P450基因发生了下调,其中CYP389A6上调倍数最高[log2ratio (RS/SS)=11.526],CYP389A2下调倍数最高[log2ratio(RS/SS)=- 12.683],由此推断,CYP389A6上调和CYP389A2下调可能是橘全爪螨对双甲脒产生抗性的重要原因.

  4. Pesticide susceptibility and carboxylesterase activity in two field populations of Panonychus citri(Acari: Tetranychidae)%柑橘全爪螨两个田间种群抗性监测及羧酸酯酶生化特性研究

    张昆; 丁天波; 杨爵铭; 豆威; 王进军

    2013-01-01

    采用叶碟浸渍法测定了重庆北碚和万州地区柑橘全爪螨Panonychus citri(McGregor)田间种群对阿维菌素、毒死蜱、甲氰菊酯和哒螨灵的抗性水平.结果表明,同室内敏感品系相比,北碚种群对毒死蜱、甲氰菊酯和哒螨灵的相对抗性水平分别达到3倍、3倍和22倍;万州种群对阿维菌素、毒死蜱、甲氰菊酯和哒螨灵的相对抗性水平分别达到2倍、35倍、10倍和2倍.柑橘全爪螨2个地理种群的羧酸酯酶CarE的生化特性研究发现,CarE酶活的增高和毒死蜱的抗性存在一定的相关性.毒死蜱对不同地理种群柑橘全爪螨CarE的抑制效果不同,对抗性倍数较高的万州种群抑制效果最差.%Samples from two populations of the citrus red mite collected from citrus orchards were assayed for susceptibility to abamectin, chlopyrifos, fenpropathrin and pyridaben. The results indicate that the Beibei population displayed a 3-, 3- and 22-fold resistance ratio, respectively, to chlopyrifos, fenpropathrin and pyridaben compared to a susceptible strain. The Wanzhou population exhibited a 2-, 35-, 10- and 2-fold resistance ratio, respectively, to the same pesticides. Enhancement of CarE activity was involved in resistance to chlopyrifos in both populations. Inhibition of chlopyrifos activity differed between the two mite populations and was stronger in the Wanzhou population than the susceptible strain.

  5. Toxicity of L1-2 fraction of Arctium lappa against Panonychus citri (McGregor) (Acari :Tetranychidae) and its effects on several metabolic enzymes%牛蒡L1-2组分对桔全爪螨的毒性和几种代谢酶的作用

    胡军华; 马丽娜; 冉春; 李鸿筠; 姚廷山; 刘浩强; 雷慧德

    2010-01-01

    [目的]探讨杀螨植物牛蒡Arctium lappa L.提取物中主要杀螨成分L1-2的杀螨作用机理.[方法]采用叶片浸渍法处理桔全爪螨Panonychus citri雌成螨后,测定了静止期、兴奋期、痉挛期、麻痹期、复苏和死亡期5个中毒阶段试虫体内几种代谢酶的活性.[结果]L1-2组分在静止期和复苏期对羧酸酯酶(carboxylesterase,CarE)具有一定的抑制作用,在其他时期均激活CarE活性.除了静止期外,在其他时期均能激活乙酰胆碱酯酶(acetylcholinesterase,AChE)和谷胱甘肽转移酶(glutathione S-transferases,GSTs)的活性,在痉挛期和麻痹期活性增强,随后在麻痹期和复苏期降低.[结论]L1-2组分对CarE的抑制与其毒杀活性有关,而中毒试虫的复苏可能与AChE和GSTs有关.该组分可在较长时期内影响桔全爪螨的神经传导及消化和生殖系统,具有潜在的应用研究价值.

  6. Toxicidade diferencial de agrotóxicos utilizados em citros para Neoseiulus californicus, Euseius concordis e Brevipalpus phoenicis

    Marcos Zatti da Silva

    2011-01-01

    Full Text Available Brevipalpus phoenicis (Geijskes (Acari: Tenuipalpidae é considerada uma das principais pragas em citros no Brasil, devido à transmissão do vírus da leprose dos citros e à necessidade de frequentes aplicações de acaricidas para seu controle. Assim, objetivou-se comparar a toxicidade dos principais agrotóxicos utilizados em citros no Brasil, sobre populações de B. phoenicis e de ácaros predadores das espécies Neoseiuluscalifornicus (McGregor e Euseius concordis (Chant (Acari: Phytoseiidae. Foram avaliadas duas populações de E. concordis: uma procedente de Jaboticabal (SP e outra de Descalvado (SP. Arenas de folhas (para fitoseídeos ou frutos (para B. phoenicis de Citrus sinensis Osbeck (L. cv. Pera, contendo 25 fêmeas adultas de cada espécie foram pulverizadas em torre de Potter. As avaliações de mortalidade foram realizadas 72 horas após a aplicação. Foram utilizadas 4 a 6 concentrações de cada produto (abamectina, acefato, azociclotina, bifentrina, clorfenapir, dicofol, dinocape, óxido de fembutatina, propargito e piridabem para a obtenção das curvas de concentração-resposta. Abamectina, azociclotina, clorfenapir, dicofol, dinocape, óxido de fembutatin, propargito e piridabem foram altamente tóxicos a B. phoenicis, com valores de CL90 iguais ou inferiores aos das concentrações recomendadas desses produtos. N. californicus foi mais tolerante a bifentrina, propargito, óxido de fembutatina e dinocape que B. phoenicis e E. concordis (ambas as populações. Esses acaricidas tiveram baixa toxicidade aos adultos de N. californicus, para o qual as CL50 foram pelo menos seis vezes maiores que suas concentrações recomendadas. Acefato foi extremamente tóxico a E. concordis, mas inócuo a adultos de N. californicus. A população de E. concordis de Jaboticabal foi mais resistente que a de Descalvado a azociclotina, bifentrina, clorfenapir, dicofol e propargito.

  7. Efeito da solarização e da adubação sobre artrópodes em solo cultivado com alface Solarization, organic and chemical fertilization combined effects on arthropods community in soil cultivated with lettuce

    Marlene G da Silva

    2009-12-01

    Full Text Available Avaliou-se o efeito da solarização e da adubação química e orgânica na comunidade de artrópodes de solo na cultura da alface, cv. Verônica. Foram conduzidos dois experimentos, em blocos ao acaso, um com solarização e o outro sem solarização, em casa de vegetação, ambos com cinco tratamentos: adubação orgânica; nitrogênio na forma amoniacal (NH4; adubação com NPK; adubação orgânica + NPK; testemunha (sem adubação. No experimento solarizado, o solo foi coberto durante 132 dias com plástico transparente. Os artrópodes foram coletados por meio de armadilhas tipo alçapão em três épocas (antes da implantação do experimento, após a solarização e após a colheita. Foram utilizados índices faunísticos e de diversidade em cada experimento, tratamento e épocas. Os principais grupos coletados foram: Collembola (82,8%, Acari (7,1%, Hymenoptera (6,1% Coleoptera (1,3% e outros (2,7%. A classe Collembola foi a mais abundante, mesmo na colheita, quando a subordem Acari aumentou substancialmente. As espécies de Collembola predominantes foram: Proisotoma tenella (Reuter (34,3%; Seira atrolutea (Arlé (29%; Folsomides centralis (Denis (7,2%; Isotomurus sp. 161 (2,4% e Sminthurides sp. 98 (1,6%. Observou-se que a diversidade e abundância da comunidade de artrópodes não foi influenciada pela solarização ou adubação.To evaluate the effect of solarization, chemical and organic fertilization on soil arthropods community in lettuce, two blocks plots trials were carried out in Brasília, Brazil. One research was conducted with solarization and the other without solarization in a soil cultivated with lettuce, cv Veronica, under protected cultivation. Each trial consisted of five fertilization treatments: T1= organic fertilization; T2= amoniacal nitrogen (ammonium sulphate; T3= chemical fertilization; T4= organic and chemical fertilization; T5= control (without fertilization. In the solarized trial, the soil was covered for 132

  8. Evaluation of Antioxidant and Antiproliferative Properties of Three <em>Actinidia> (<em>Actinidia> <em>kolomikta>, <em>Actinidia argutaem>, <em>Actinidia> <em>chinensis> Extracts <em>in Vitroem>

    Jia-Ren Liu

    2012-05-01

    Full Text Available The total phenolic content, total flavonoid content, vitamin C content, and antioxidant activities of ethanol extracts from different kiwifruit varieties (<em>Actinidia> <em>kolomikta>, <em>Actinidia argutaem>, <em>Actinidia> <em>chinensis> were determined in this study. Multiple scavenging activity assays including the hydroxyl radical, O2·radical, DPPH, and the ABTS+ radical scavenging activity assays were used to identify the antioxidant activities of <em>Actinidia> extracts. The cell viability of HepG2 and HT-29 cells was also examined in this study. The results demonstrated that the <em>Actinidia kolomiktaem> extract had a higher antioxidant activity than the other two <em>Actinidia> extracts. There is a positive correlation between antioxidant activity and the polyphenols and vitamin C content in all three extracts (<em>R>2 ≥ 0.712, <em>p> em>< 0.05. The <em>Actinidia argutaem> extract had the highest inhibitory effect on HepG2 and HT-29 cell growth. These results provide new insight into the health functions of fruit and demonstrate that <em>Actinidia> extracts can potentially have health benefits.

  9. <em>α>-Glucosidase Inhibitory Constituents from <em>Acanthopanax senticosusem> Harm Leaves

    Hai-Xue Kuang

    2012-05-01

    Full Text Available A new triterpene glycoside, 3-<em>O-[(α>-L-rhamnopyranosyl(1→2]-[<em>β>-D-glucuronopyranosyl-6-<em>O>-methyl ester]-olean-12-ene-28-olic acid (1 and a new indole alkaloid, 5-methoxy-2-oxoindolin-3-acetic acid methyl ester (5 were isolated from the leaves of <em>Acanthopanax senticosusem> Harms along with six known compounds. The structures of the new compounds were determined by means of 2D-NMR experiments and chemical methods. All the isolated compounds were evaluated for their glycosidase inhibition activities and compound 6 showed significant <em>α>-glucosidase inhibition activity.

  10. Toxicidade de extratos de nim (Azadirachta indica ao ácaro-vermelho-do-cafeeiro Oligonychus ilicis Toxicity of neem extracts (Azadirachta indica to the coffee red mite Oligonychus ilicis

    Sheila Abreu Mourão

    2004-08-01

    Full Text Available O objetivo deste trabalho foi estudar a toxicidade aguda e crônica de extratos de óleo de torta, de sementes e de folhas de Azadirachta indica A. Juss (nim a fêmeas do ácaro-vermelho-do-cafeeiro Oligonychus ilicis (McGregor (Acari: Tetranychidae. A mortalidade e a taxa instantânea de crescimento populacional de fêmeas adultas desse ácaro foram avaliadas em discos de folhas de cafeeiro com 3,5 cm de diâmetro, impregnados com resíduo seco dos extratos de nim e flutuando em água. As concentrações dos extratos de óleo de torta, sementes e folhas de nim que mataram 50% e 99% dos indivíduos de O. ilicis, após 72 horas de exposição, foram de 0,02, 15,9 e 121,4 mg/mL e de 10,9, 520,9 e 277,4 mg/mL, respectivamente. A taxa instantânea de crescimento populacional de O. ilicis diminuiu, linearmente, com o aumento da concentração dos extratos de óleo de torta, sementes e folhas de nim até 0,075, 15 e 144 mg/mL, respectivamente, a partir das quais as populações desse ácaro foram extintas.The objective of this work was to study the toxicity of extracts of oil cake, seeds and leaves of Azadirachta indica A. Juss (neem to the coffee red mite Oligonychus ilicis (McGregor (Acari: Tetranychidae. Concentration-mortality and instantaneous rate of populational growth were determined for adult females of the red mite on disks of coffee with 3.5 cm diameter, floating on water and impregnated with dry residues of the neem extracts. Concentrations of these extracts which caused 50% mortality of the individuals of O. ilicis were 0.02, 15.9 and 121.4 mg/mL; while 99% mortality of this mite was reached with 10.9, 520.9 and 277.4 mg/mL for oil cake, seeds and leaves extracts, respectively. The instantaneous rate of populational growth of O. ilicis dropped as the extract doses increased up to 0.075, 15 and 144 mg/mL for oil cake, seeds and leaves extracts, respectively.

  11. New records of marine water mites (Acari: Hydrachnidia: Pontarachnidae) from the Pujada Bay (West Pacific Ocean) and the Andaman Sea (Indian Ocean)

    Pesic, V.; Chatterjee, T.; Troch, M. de; Ingole, B.S.

    entomologica serbica, 2009, 14(1): 129-132 UDC 595.42(265.5+267) [Short communication] NEW RECORDS OF MARINE WATER MITES (ACARI: HYDRACHNIDIA: PONTARACHNIDAE) FROM THE PUJADA BAY (WEST PACIFIC OCEAN) AND THE ANDAMAN SEA (INDIAN OCEAN) VLADIMIR PEŠIĆ1, TAPAS... CHATTERJEE2, MARLEEN DE TROCH3 and BABAN INGOLE4 1 University of Montenegro, Department of Biology, Cetinjski put b.b., 81000 Podgorica, Montenegro E-mail: pesicv@t-com.me 2 Indian School of Learning, Department of Biology, I.S.M. Annexe, P.O. – I...

  12. Three New Myrsinol Diterpenes from <em>Euphorbia proliferaem> and Their Neuroprotective Activities

    Yuanqiang Guo

    2012-08-01

    Full Text Available Three new myrsinol diterpenes were isolated from the roots of<em> em>>Euphorbia proliferaem>. Their structures were elucidated as 2<em>α-O>-isobutyryl-3<em>β>,5<em>α>,7<em>β>,10,15<em>β-penta-O>-acetyl-14<em>α-O>-benzoyl-10,18-dihydromyrsinol (1, 2<em>α-O>-isobutyryl-3<em>β-O>-propion-yl-5<em>α>,7<em>β>,10,15<em>β-tetra-O>-acetyl-10,18-dihydromyrsinol (2, and 2<em>α>,14<em>α-di-O>-benzoyl-3<em>β>,5<em>α>,7<em>β>,10,15<em>β-penta-O>-acetyl-10,18-dihydromyrsinol (3 on the basis of spectroscopic data analyses (IR, ESI-MS, HR-ESI-MS, and 1D and 2D NMR. Their neuroprotective activities were evaluated and compounds 1 and 2 showed neuroprotective effects against MPP+-induced neuronal cell death in SH-SY5Y cells.

  13. Larvicidal potencial of Sapindus saponaria (Sapindaceae against Rhipicephalus sanguineus (Latreille, 1806 (Acari: Ixodidae Potencial larvicida de Sapindus saponaria (Sapindaceae sobre Rhipicephalus sanguineus (Latreille, 1806 (Acari: Ixodidae

    F.F. Fernandes

    2007-02-01

    Full Text Available The larvicidal potential of the crude ethanolic extracts (CEE of the stem peel of Sapindus saponaria was evaluated against Rhipicephalus sanguineus. Lethal concentrations (LC, were calculated by preparing CEE solutions at different concentrations in distilled water. Larvae fasted for 14-21 days were utilized in the bioassays, after incubation of engorged females collected from infested environments frequented by dogs in several neighborhoods of Goiânia, GO. Bioassays were performed in a specially constructed biological chamber for testing botanical acaricides, acclimatized to 27±1ºC, RH>80%. The larvae were counted on filter paper envelopes impregnated with the solutions or distilled water and larval mortality observed after 48h. S. saponaria showed good larvicidal activity (LC50 and LC99 of 1994 and 3922ppm, respectively and the results demonstrated its potential as a botanical acaricide and an alternative control measure for R. sanguineus.Avaliou-se a potencialidade larvicida do extrato-bruto etanólico (EBE da casca do caule de Sapindus saponaria sobre Rhipicephalus sanguineus. Para o cálculo das concentrações letais (CL foram preparadas soluções com diferentes concentrações do EBE dissolvido em água destilada. Foram utilizadas larvas em jejum com 14 a 21 dias, obtidas pela incubação de teleóginas, coletadas em ambientes infestados, freqüentados por cães de vários bairros de Goiânia. Os bioensaios foram realizados em uma câmara biológica para testes com acaricidas botânicos, climatizada a 27±1ºC e UR>80%. As larvas foram contidas em envelopes de papel filtro impregnados com as soluções (grupo teste ou com água destilada (grupo-controle e a mortalidade larval foi observada após 48h. S. saponaria demonstrou atividade larvicida satisfatória (CL50 e CL99 respectivamente de 1994 e 3922ppm e os resultados demonstraram seu potencial como acaricida botânico e medida alternativa para o controle de R. sanguineus.

  14. Diversidade e dinâmica populacional de ácaros em pomar cítrico Diversity and population dynamics of mites in citrus orchard

    Marcos Zatti da Silva

    2012-01-01

    Full Text Available As plantas de citros hospedam diversas espécies de ácaros que causam sérios prejuízos à produção e qualidade de frutos no Brasil. Esta pesquisa teve por objetivo estudar a diversidade de ácaros e a dinâmica populacional das principais espécies de ácaros em pomar de citros, Citrus sinensis (L. Osbeck, localizado no município de Descalvado, Estado de São Paulo. Foram realizadas 14 avaliações em um período de 12 meses, de fevereiro de 2007 a fevereiro de 2008. Em cada avaliação, foram coletadas dez folhas e cinco frutos na parte interna de cada uma das 40 plantas demarcadas no pomar. Foram encontradas 34 espécies de ácaros, distribuídas em 17 famílias. As espécies mais abundantes foram: Phyllocoptruta oleivora (Ashmed (Eriophyidae, Panonychus citri (McGregor (Tetranychidae, Brevipalpus phoenicis (Geijskes (Tenuipalpidae e Fungitarsonemus sp. (Tarsonemidae. Entre os ácaros predadores, a família Phytoseiidae foi a de maior riqueza (oito espécies e abundância (1.072 indivíduos. A espécie predominante foi Euseius concordis (Chant, correspondendo a 98,3% dos ácaros desta família. Foram observadas correlações significativas entre densidades populacionais de E. concordis e dos ácaros fitófagos P. citri, B. phoenicis e P. oleivora. Também foram observadas correlações significativas entre densidades populacionais desses ácaros e fatores meteorológicos como temperatura e precipitação pluvial. Os resultados indicam que E. concordis atua como inimigo natural de P. citri, B. phoenicis e P. oleivora no pomar cítrico estudado.The citrus plants are hosts of several species of mites, which cause severe damage to production and quality of fruits in Brazil. The objective of this research was to study the diversity of mites and population dynamics of the main mite species in a citrus orchard, Citrus sinensis (L. Osbeck, in Descalvado County, State of São Paulo, Brazil. Fourteen evaluations were conducted over a period of 12

  15. Ethanol extract of Cymbopogon winterianus on mortality and number of eggs of Tetranychus urticae

    Victor Bernardo Vicentini; Dirceu Pratissoli; Vagner Tebaldi de Queiroz; Adilson Vidal Costa; Patrícia Fontes Pinheiro; Fernando Domingo Zinger; Vando Miossi Rondelli

    2015-01-01

    Plant extracts have been studied as a promising source of natural insecticides. This study assessed the effect of the ethanol extract of Cymbopogon winterianus Jowitt (citronella grass) in comparison with an insecticide containing azadirachtin (ICA) on mortality and number of eggs of Tetranychus urticae Koch (Acari: Tetranychidae) in laboratory conditions. For the tests, the mites were sprayed with the aid of a Potter spray tower. To assess the mortality of females, LC50 value for extract of ...

  16. Fitoseídeos (Acari: Phytoseiidae associados a cafezais e fragmentos florestais vizinhos Phytoseiids (Acari: Phytoseiidae associated to coffee plantations and adjacent forest fragments

    Ester Azevedo Silva

    2010-10-01

    Full Text Available Existem poucas informações sobre a fauna de ácaros predadores (Phytoseiidae em ambientes naturais brasileiros adjacentes a agroecossistemas cafeeiros (Coffea spp. ou sobre a influência que essa vegetação exerce como reservatório de ácaros predadores. Neste estudo, objetivou-se avaliar a diversidade destes organismos em cafeeiros e fragmentos florestais adjacentes. Coletaram-se amostras das espécies Calyptranthes clusiifolia (Miq. O. Berg (Myrtaceae, Esenbeckia febrifuga (A. St.-Hil. A. Juss. ex Mart. (Rutaceae, Metrodorea stipularis Mart. (Rutaceae e Allophylus semidentatus (Miq. Radlk. (Sapindaceae, em oito fragmentos florestais, de 5 a 51 ha, e cafezais adjacentes, nos meses de junho (final período chuvoso e outubro (final período seco nos anos 2004 e 2005, na região Sul do Estado de Minas Gerais. Ácaros foram extraídos das folhas, utilizando o método de lavagem e, em seguida, montados em lâminas de microscopia em meio de Hoyer, para identificação específica. No total foram identificados 2.348 fitoseídeos, sendo 2.090 nos fragmentos florestais e 258 espécimes nos cafezais adjacentes, pertencentes a 38 espécies. Servindo-se de análise faunística, a espécie Iphiseiodes zuluagai Denmark & Muma, 1972 apresentou os melhores índices no agroecossistema cafeeiro, sendo muito frequente e constante nas épocas estudadas. Nos fragmentos florestais Amblyseius herbicolus Chant, 1959, Iphiseiodes affs. neonobilis Denmark & Muma, 1978, Leonseius regularis DeLeon, 1965 e Euseius alatus DeLeon, 1966 foram dominantes, muito abundantes, muito frequentes e constantes nas épocas estudadas. Podemos concluir que a vegetação nativa abriga ácaros predadores, inimigos naturais de ácaros-praga, que ocorrem na cultura cafeeira, possibilitando o desenvolvimento de programas de manejo ecológico com áreas de vegetação natural e agroecossistemas cafeeiros adjacentes.There is little information about the fauna of predatory mites

  17. Neonatal Phosphate Nutrition Alters <em>in em>Vivo> and <em>in em>Vitro> Satellite Cell Activity in Pigs

    Chad H. Stahl

    2012-05-01

    Full Text Available Satellite cell activity is necessary for postnatal skeletal muscle growth. Severe phosphate (PO4 deficiency can alter satellite cell activity, however the role of neonatal PO4 nutrition on satellite cell biology remains obscure. Twenty-one piglets (1 day of age, 1.8 ± 0.2 kg BW were pair-fed liquid diets that were either PO4 adequate (0.9% total P, supra-adequate (1.2% total P in PO4 requirement or deficient (0.7% total P in PO4 content for 12 days. Body weight was recorded daily and blood samples collected every 6 days. At day 12, pigs were orally dosed with BrdU and 12 h later, satellite cells were isolated. Satellite cells were also cultured <em>in vitroem> for 7 days to determine if PO4 nutrition alters their ability to proceed through their myogenic lineage. Dietary PO4 deficiency resulted in reduced (<em>P> < 0.05 sera PO4 and parathyroid hormone (PTH concentrations, while supra-adequate dietary PO4 improved (<em>P> < 0.05 feed conversion efficiency as compared to the PO4 adequate group. <em>In vivoem> satellite cell proliferation was reduced (<em>P> < 0.05 among the PO4 deficient pigs, and these cells had altered <em>in vitroem> expression of markers of myogenic progression. Further work to better understand early nutritional programming of satellite cells and the potential benefits of emphasizing early PO4 nutrition for future lean growth potential is warranted.

  18. Toxicity of acaricides to Raoiella indica and their selectivity for its predator, Amblyseius largoensis (Acari: Tenuipalpidae: Phytoseiidae).

    de Assis, Carla P O; de Morais, Elisângela G F; Gondim, Manoel G C

    2013-07-01

    Raoiella indica Hirst (Acari: Tenuipalpidae) is considered a pest of coconut palm in Asia and the Middle East. This mite was recently introduced in the Americas, where it spread to several countries and expanded its range of hosts, causing heavy losses to coconut and banana production. The phytoseiid mite Amblyseius largoensis (Muma) is one of the predators most often encountered in coconut palms. Because the current prospects for the control of R. indica in the New World indicate the use of acaricides and the management of their natural enemies, the objective of this study was to evaluate the toxicity of selected acaricides to R. indica and the selectivity (i.e., toxicity to the predator relative to toxicity to the prey) for A. largoensis. Assays were performed by the immersion of banana leaf discs in acaricide solutions, followed by the placing of adult females of the pest or predator on the discs. Mortality of the mites was evaluated after 24 h, and the data obtained were subjected to probit analysis. Abamectin, fenpyroximate, milbemectin and spirodiclofen were the products most toxic to R. indica adults, whereas fenpyroximate and spirodiclofen were the most selective for A. largoensis. PMID:23229493

  19. New Miticides for Integrated Pest Management of Varroa destructor (Acari: Varroidae) in Honey Bee Colonies on the Canadian Prairies.

    Vandervalk, L P; Nasr, M E; Dosdall, L M

    2014-12-01

    Varroa destructor Anderson and Trueman 2000 (Acari: Varroidae) is an ectoparasitic mite of the honey bee, Apis mellifera L. (Hymenoptera: Apidae). Honey bee colonies require extensive management to prevent mortality caused by varroa mites and the viruses they vector. New miticides (Thymovar and HopGuard) to manage varroa mites were evaluated during the spring and fall treatment windows of the Canadian prairies to determine their effectiveness as part of an integrated management strategy. Thymovar and HopGuard were evaluated alongside the currently used industry standards: Apivar and formic acid. Results demonstrated that Apivar and formic acid remain effective V. destructor management options under spring and fall conditions. Applications of Thymovar during spring were associated with a reduction in brood area, and therefore should be limited to the fall season. The miticide HopGuard was not effective in managing V. destructor, and alteration of the current delivery system is necessary. This study demonstrates the potential for new effective treatment options to supplement currently used V. destructor integrated pest management systems. PMID:26470066

  20. Constituents from <em>Vigna em>vexillata> and Their Anti-Inflammatory Activity

    Guo-Feng Chen

    2012-08-01

    Full Text Available The seeds of <em>Vigna em>genus are important food resources and there have already been many reports regarding their bioactivities. In our preliminary bioassay, the chloroform layer of methanol extracts of<em> V. vexillata em>demonstrated significant anti-inflammatory bioactivity. Therefore, the present research is aimed to purify and identify the anti-inflammatory principles of <em>V. vexillataem>. One new sterol (1 and two new isoflavones (2,3 were reported from the natural sources for the first time and their chemical structures were determined by the spectroscopic and mass spectrometric analyses. In addition, 37 known compounds were identified by comparison of their physical and spectroscopic data with those reported in the literature. Among the isolates, daidzein (23, abscisic acid (25, and quercetin (40 displayed the most significant inhibition of superoxide anion generation and elastase release.

  1. Distribuição espacial de Calacarus Heveae feres na cultura da seringueira em Marinópolis - São Paulo

    Gustavo Luís Mamoré Martins

    2012-04-01

    Full Text Available O objetivo deste trabalho foi estudar a distribuição espacial de Calacarus heveae Feres (Acari: Eriophyidae na cultura da seringueira, tendo como base um monitoramento realizado com lupa de bolso. A área experimental, localizada em Marinópolis, São Paulo, com 1.000 plantas do clone RRIM 600, foi dividida em 100 parcelas de 10 plantas cada uma. Foram realizadas 16 coletas no período de dezembro de 2007 a junho de 2008. Nas coletas foram amostradas duas plantas por parcela, retirando-se de cada uma extremidade de ramo com 30 cm de comprimento. No laboratório, os ácaros foram avaliados com o uso de lupa de bolso de 20X de aumento, em seis folíolos por parcela, sendo três de cada ramo. A contagem dos ácaros foi realizada em duas áreas de 1 cm² na página superior dos folíolos, uma de cada lado da nervura principal. Os índices de dispersão estudados foram: razão variância/média (I, índice de Morisita (Id, coeficiente de Green (Cx e expoente k da distribuição binomial negativa. C. heveae apresenta distribuição agregada em área de cultivo de seringueira. A distribuição espacial do ácaro ajusta-se ao modelo de distribuição binomial negativa.

  2. The Antimicrobial Efficacy of <em>Elaeis guineensisem>: Characterization, <em>in Vitroem> and <em>in Vivoem> Studies

    Sreenivasan Sasidharan

    2012-04-01

    Full Text Available The urgent need to treat multi-drug resistant pathogenic microorganisms in chronically infected patients has given rise to the development of new antimicrobials from natural resources. We have tested <em>Elaeis guineensis em>Jacq> em>(Arecaceae methanol extract against a variety of bacterial, fungal and yeast strains associated with infections. Our studies have demonstrated that <em>E. guineensisem> exhibits excellent antimicrobial activity <em>in vitroem> and <em>in vivoem> against the bacterial and fungal strains tested. A marked inhibitory effect of the <em>E. guineensisem> extracts was observed against <em>C. albicansem> whereby <em>E. guineensisem> extract at ½, 1, or 2 times the MIC significantly inhibited <em>C. albicansem> growth with a noticeable drop in optical density (OD of the bacterial culture. This finding confirmed the anticandidal activity of the extract on <em>C. albicansem>. Imaging using scanning (SEM and transmission (TEM electron microscopy was done to determine the major alterations in the microstructure of the extract-treated <em>C. albicansem>. The main abnormalities noted via SEM and TEM studies were the alteration in morphology of the yeast cells. <em>In vivoem> antimicrobial activity was studies in mice that had been inoculated with <em>C. albicansem> and exhibited good anticandidal activity. The authors conclude that the extract may be used as a candidate for the development of anticandidal agent.<em> em>

  3. Evaluation of Pseudopteroxazole and Pseudopterosin Derivatives against <em>Mycobacterium> <em>tuberculosis> and Other Pathogens

    Malcolm W. B. McCulloch

    2012-08-01

    Full Text Available Pseudopterosins and pseudopteroxazole are intriguing marine natural products that possess notable antimicrobial activity with a commensurate lack of cytotoxicity. New semi-synthetic pseudopteroxazoles, pseudopteroquinoxalines and pseudopterosin congeners along with simple synthetic mimics of the terpene skeleton were synthesized. In order to build structure-activity relationships, a set of 29 new and previously reported compounds was assessed for <em>in> <em>vitro> antimicrobial and cytotoxic activities. A number of congeners exhibited antimicrobial activity against a range of Gram-positive bacteria including <em>Mycobacterium> <em>tuberculosis> H37Rv, with four displaying notable antitubercular activity against both replicating and non-replicating persistent forms of <em>M.> <em>tuberculosis>. One new semi-synthetic compound, 21-((1<em>H>-imidazol-5-ylmethyl-pseudopteroxazole (7a, was more potent than the natural products pseudopterosin and pseudopteroxazole and exhibited equipotent activity against both replicating and non-replicating persistent forms of <em>M.> <em>tuberculosis> with a near absence of <em>in> <em>vitro> cytotoxicity. Pseudopteroxazole also exhibited activity against strains of <em>M.> <em>tuberculosis> H37Rv resistant to six clinically used antibiotics.

  4. Occurrence and within-plant distribution of red mite in cotton upland = Ocorrência e distribuição vertical de ácaro-vermelho em algodoeiro herbáceo.

    João Gutemberg Leite Moraes

    2014-12-01

    Full Text Available The spider mites in the genus Tetranychidae are among the main arthropod pests of cotton. The occurrence of these pests in this malvaceae may cause yield reduction, increase of control costs, and also impact in the environment and on applicators health. The aim of this study was to verify the taxonomic identity of a red spider mite; define a sampling method for this arthropod and a sampling unit for the spider mite on cotton. This study was done in an area belonging to the Agronomy Department of the Federal University of Ceará, Fortaleza, Brazil. The CNPA-2H cotton cultivar was used. Initially, the spider mite was collected for identification, stored in a 70% alcohol solution and send for identification. Later on, the sampling method was tested in which the scouts used a score scale. Next, the within-plant distribution of the cotton red spider mite was studied to determine the sampling unit for this spider. Later on, by using a score sampling method, the within-plant distribution was studied to determine sample unit. A factorial 3x8 in a complete random design was used, with 10 replicates. Scores were transformed to infestation grade and used in analysis. The cotton red spider mite corresponded to Tetranychus aff. yusti McGregor. It was found that the methodology was well suited to discriminate mite in within-plant distribution and that the fourth, leaf top to down, was the ideal sampling unit. = Os ácaros do gênero Tetranychidae estão entre os principais artrópodes-pragas da cultura do algodoeiro. A ocorrência dessas pragas nessa malvácea pode ocasionar perdas no rendimento da cultura, inclusive pelo aumento nos custos de controle, o que pode implicar impactos no meio-ambiente e na saúde dos aplicadores de defensivos. Objetivou-se com esta pesquisa verificar a identidade taxonômica de um ácaro-vermelho, definir uma metodologia para a avaliação desse artrópode e definir uma unidade amostral para o ácaro no algodoeiro. O estudo foi

  5. Efficient Heterologous Transformation of <em>Chlamydomonas> reinhardtiiem> <em>npq2em> Mutant with the Zeaxanthin Epoxidase Gene Isolated and Characterized from<em> Chlorella zofingiensisem>

    Herminia Rodríguez

    2012-09-01

    Full Text Available In the violaxanthin cycle, the violaxanthin de-epoxidase and zeaxanthin epoxidase catalyze the inter-conversion between violaxanthin and zeaxanthin in both plants and green algae. The zeaxanthin epoxidase gene from the green microalga <em>Chlorella zofingiensisem> (<em>Czzep> has been isolated<em>. em>This gene encodes a polypeptide of 596 amino acids. A single copy of <em>Czzep> has been found in the <em>C. zofingiensisem> genome by Southern blot analysis. qPCR analysis has shown that transcript levels of <em>Czzep> were increased after zeaxanthin formation under high light conditions. The functionality of <em>Czzep> gene by heterologous genetic complementation in the <em>Chlamydomonas> mutant <em>npq2em>, which lacks zeaxanthin epoxidase (ZEP activity and accumulates zeaxanthin in all conditions, was analyzed. The <em>Czzep> gene was adequately inserted in the pSI105 vector and expressed in <em>npq2em>. The positive transformants were able to efficiently convert zeaxanthin into violaxanthin, as well as to restore their maximum quantum efficiency of the PSII (Fv/Fm. These results show that <em>Chlamydomonas> can be an efficient tool for heterologous expression and metabolic engineering for biotechnological applications.

  6. Ácaros predadores (Acari em plantas nativas e cultivadas do Estado do Rio Grande do Sul, Brasil Predators mites (Acari in native and cultivated plants of the State of Rio Grande do Sul, Brazil

    Noeli Juarez Ferla

    2002-12-01

    Full Text Available This research was carried out in twenty counties of the following regions in the state of Rio Grande do Sul: Plain, Central Depression, Plateau and Coast Plain to find out the diversity of mite predators in these places. Forty-six vegetable species were sampled, thirty species of miles of the families Anystidae, Ascidae, Cheyletidae, Cunaxidae, Phyloseiidae and Stigmaeidae were mel. The Phytoseiidae were the mite that presented the greatest diversity, being present in the majority of the sample plants. Most of the Phytoseiidae that were met belong to five species of the Euseius Wainstein, 1962 genus, the second genus of this family was Iphiseiodes DeLeon, 1966, with just one species. The Stigmaeidae come up as second family in number but fewer than Phytoseiidae. In this family, the most common mite belong to the Agistemus Sumers, 1960 genus. The biggest of the mites species (13 species, was met in Morus spp. (Moraceae and Tabebuia spp. (Bignoniaceae; Phaseolus vulgaris (Papilionaceae; only one species of the mite was met in Campomanesia spp. (Myrtaceae, Phaseolus vulgaris (Papilionaceae and Rosa spp. (Rosaceae. In Alamanda spp.(Apocinaceae, Ficus spp. (Moraceae, Jacaranda mimosifolia (Bignoniaceae and Solanum spp. (Solanaceae were met mites predators. A dichotomic key is presented to separate the families, genus and species of the mites.

  7. Ocorrência de Ixodes loricatus Neumann, 1899 (Acari: Ixodidae) parasitando Didelphis albiventris (Lund, 1841), (Didelphimorphia: Didelphidae), em Campo Grande, MS Occurrence of Ixodes loricatus Neumann, 1899 (Acari: Ixodidae) parasitizing Didelphis albiventris (Lund, 1841) (Didelphimorphia: Didelphidae) in Campo Grande , MS

    Soraya R. Miziara; Fernando Paiva; Renato Andreotti; Wilson W. Koller; Vinicius A. Lopes; Nara T. Pontes; Klaudia Bitencourt

    2008-01-01

    Este é primeiro relato da ocorrência de Ixodes no Estado do Mato Grosso do Sul e primeiro caso identificado de Ixodes loricatus Neumann, 1899, parasitando Didelphis albiventris no município de Campo Grande, MS, Brasil.This is first report of occurence of Ixodes in the State of Mato Grosso do Sul and first description of Ixodes loricatus Neumann, 1899, parasitizing Didelphis albiventris in Campo Grande, MS, Brazil.

  8. Toxicidade residual de alguns agrotóxicos recomendado na agricultura sobre Neoseiulus californicus (McGregor) (Acari: Phytoseiidae) Residual toxicity of some pesticides recommended for citrus orchards on the predaceous mite Neoseiulus californicus (McGregor) (Acari: phytoseiidae)

    Marcos Zatti da Silva; Caralos Amadeu Leite de Oliveira

    2007-01-01

    O objetivo do trabalho foi avaliar a toxicidade residual de alguns agrotóxicos utilizados em citros sobre Neoseiulus californicus (McGregor) em condições de laboratório. O método de bioensaio adotado foi o de contato residual. Folhas de citros da variedade Pêra, acondicionadas em arenas, foram pulverizadas em torre de Potter. A toxicidade residual dos produtos foi avaliada duas horas e 1; 3; 5; 7; 10; 14 e 21 dias após a aplicação. Em cada arena, foram transferidas dez fêmeas adultas de N. ca...

  9. Reference Gene Selection in the Desert Plant <em>Eremosparton songoricuem>m>

    Dao-Yuan Zhang; Yuan-Ming Zhang; Wood, Andrew J.; Xiao-Shuang Li; Hong-Lan Yang

    2012-01-01

    <em>Eremosparton songoricum em>(Litv.) Vass. (<em>E. songoricumem>) is a rare and extremely drought-tolerant desert plant that holds promise as a model organism for the identification of genes associated with water deficit stress. Here, we cloned and evaluated the expression of eight candidate reference genes using quantitative real-time reverse transcriptase polymerase chain reactions. The expression of these candidate reference genes was analyzed in a diverse set...

  10. CARACTERIZAÇÃO QUÍMICA E EFEITO DO ÓLEO ESSENCIAL DE ERVA-DE-SANTA-MARIA SOBRE O ÁCARO-RAJADO DE MORANGUEIRO

    JOÃO PAULO PEREIRA PAES

    2015-06-01

    Full Text Available O ácaro-rajado, Tetranychus urticae Koch (1836 (Acari: Tetranychidae, é a principal praga na cultura do morangueiro. O objetivo deste trabalho foi a caracterização química do óleo essencial de Chenopodium ambrosioides L. e a avaliação de seus efeitos sobre o ácaro-rajado. Os compostos do óleo essencial foram identificados pelasanálises de CG-DIC e CG-EM. A mortalidade e a oviposição foram avaliadas após a pulverização do óleo (2,5; 5,0; 7,5 e 10,0% v v-1 sobre fêmeas do ácaro-rajado. O rendimento do óleo essencial foi de 0,3%, e os compostos identificados foram (Z-ascaridol (87%, (E-ascaridol (5,04%, p-cimeno (4,83%, a- terpineno (1,24% e piperitona (0,7%. Os maiores valores para mortalidade e redução de oviposição observados foram 85% e 69%, respectivamente. O óleo essencial de C. ambrosioidesé uma opção promissora para o manejo do ácaro-rajado.

  11. Laboratory evaluation of a native strain of Beauveria bassiana for controlling Dermanyssus gallinae (De Geer, 1778) (Acari: Dermanyssidae).

    Immediato, Davide; Camarda, Antonio; Iatta, Roberta; Puttilli, Maria Rita; Ramos, Rafael Antonio Nascimento; Di Paola, Giancarlo; Giangaspero, Annunziata; Otranto, Domenico; Cafarchia, Claudia

    2015-09-15

    The poultry red mite, Dermanyssus gallinae (De Geer, 1778) (Acari: Dermanyssidae) is one of the most economically important ectoparasites of laying hens worldwide. Chemical control of this mite may result in environmental and food contamination, as well as the development of drug resistance. High virulence of Beauveria bassiana sensu lato strains isolated from naturally infected hosts or from their environment has been demonstrated toward many arthropod species, including ticks. However, a limited number of studies have assessed the use of B. bassiana for the control of D. gallinae s.l. and none of them have employed native strains. This study reports the pathogenicity of a native strain of B. bassiana (CD1123) against nymphs and adults of D. gallinae. Batches of nymph and adult mites (i.e., n=720 for each stage) for treated groups (TGs) were placed on paper soaked with a 0.1% tween 80 suspension of B. bassiana (CIS, 10(5), 10(7) and 10(9) conidia/ml), whilst 240 untreated control mites for each stage (CG) were exposed only to 0.1% tween 80. The mites in TG showed a higher mortality at all stages (p<0.01) when compared to CG, depending on the time of exposure and the conidial concentration. A 100% mortality rate was recorded using a CIS of 10(9) conidia/ml 12 days post infection (DPI) in adults and 14 DPI in nymphs. B. bassiana suspension containing 10(9) conidia/ml was highly virulent towards nymph and adult stages of D. gallinae, therefore representing a possible promising natural product to be used in alternative or in combination to other acaricidal compounds currently used for controlling the red mite. PMID:26206607

  12. Seasonal variation of North American form of Gigantolaelaps mattogrossensis (Acari: Laelapidae) on marsh rice rat in southern coastal Texas.

    Carmichael, Joseph A; Strauss, Richard E; McIntyre, Nancy E

    2007-01-01

    The ectoparasites of a small mammal community within an intertidal zone in the upper Gulf coast region of Texas were studied to assess the seasonal variation in abundances of the mite Gigantolaelaps mattogrossensis (Fonseca) (Acari: Laelapidae) on the marsh rice rat, Oryzomys palustris (Harlan). Further study into the ecology and dynamics of this parasite-host relationship was deemed to be necessary to expand the understanding of these potential participants in the ecology of Bayou Hantavirus, an important causative agent of Hantavirus pulmonary syndrome. The objective of this study was to determine the effects of five predictor variables on mite abundance: prevalences of hosts, relative humidity, precipitation, temperature, and length of daylight. Mite abundance was modeled as a function of the five variables with analyses of variance and multiple regressions; however, because the predictor variables pertain to the sampling period rather than to the individual rodent host, the effective sample size was small and thus the sums of squares and cross products matrix was singular. We therefore developed and used a new method for estimating regression coefficients based on the "noise-addition method" (random residual variation) combined with a bootstrap step converting the reduced rank data to full rank, providing realistic estimates of confidence intervals for the regression statistics. The population abundances of mites fluctuated significantly across collecting periods. Humidity and precipitation were the most influential variables in explaining the variation in abundances of mites. Model interpretation suggests that G. mattogrossensis is a nidicolous parasite. These results provide a baseline understanding of the seasonal interactions between parasite and host. PMID:17294924

  13. Amblyomma hadanii n. sp. (Acari: Ixodidae), a tick from northwestern Argentina previously confused with Amblyomma coelebs Neumann, 1899.

    Nava, Santiago; Mastropaolo, Mariano; Mangold, Atilio J; Martins, Thiago F; Venzal, José M; Guglielmone, Alberto A

    2014-07-01

    All stages of Amblyomma hadanii n. sp. (Acari: Ixodidae) are described from northwestern Argentina. The diagnostic characters for males are a combination of the pattern of scutal ornamentation, basis capituli dorsally rectangular with cornua, coxa I with two subequal spurs (the internal wider, the external longer), coxae II-III with a single spur, coxa IV with a single spur not reaching level of anus, ventral plates irregular in shape (larger and sometimes with a small incision on festoons 4, 5 and 6) and hypostome spatulate with dental formula 3/3 in 7-8 rows. The diagnostic characters for the females are a combination of scutal ornamentation, postero-lateral margins of scutum slightly convex, coxa I with two subequal spurs (the internal wider, the external longer), basis capituli dorsally rectangular, porose areas rounded, genital aperture U-shaped, and hypostome spatulate with dental formula 3/3 in 7-8 rows. Diagnosis of nymphs can be performed by a combination of basis capituli rectangular, scutum with large punctations in the lateral fields and small punctations in the central field, and cervical groove short and ending as a small shallow depression at the eye level. Larvae are diagnosed by the shape of basis capituli, scutum with with posterior margin slightly convex, and legs with coxa I with 2 triangular spur (the external longer than the internal), and with coxae II and III each with 1 triangular spur. The hosts recorded for this new tick species are Tapirus terrestris (Linnaeus), horse, cattle, dog and humans. Analyses of a 410 bp fragment of the mitochondrial 16S rRNA gene and the complete sequence of the nuclear 18S rRNA gene supported the description of A. hadanii as a new species. PMID:24935128

  14. Indoor winter fumigation with formic acid for control of Acarapis woodi (Acari: Tarsonemidae) and nosema disease, Nosema sp.

    Underwood, Robyn M; Currie, Robert W

    2009-10-01

    Indoor fumigation of honey bees, Apis mellifera L., with formic acid to control varroa mites, Varroa destructor Anderson & Trueman, allows simultaneous fumigation of multiple colonies with little labor input and good efficacy. Several experiments were designed to test the efficacy of formic acid as a treatment for honey bee mites, Acarapis woodi (Rennie) (Acari: Tarsonemidae), and nosema disease, Nosema sp., indoors in winter. The objectives of this study were (1) to determine the efficacy of formic acid fumigation for honey bee mite control by using both the thoracic slice and live dissection methods and (2) to determine whether indoor fumigation can reliably prevent the buildup of nosema disease in overwintering honey bee colonies. Indoor winter fumigation of honey bee colonies with formic acid was effective in killing a high percentage of honey bee mites but did not significantly reduce the proportion of bees with infested tracheae over the duration of the experiments. Thus, the method used to determine the efficacy of the treatment affected the results. Under conditions of relatively low or decreasing levels of nosema, fumigation tended to suppress the mean abundance of nosema spores relative to the controls. In three separate fumigation experiments using a range of formic acid concentrations, there was no statistical difference between the buildup or maintenance of nosema spore mean abundance over the winter in bees from formic acid fumigated colonies compared with untreated controls. However, fumigation with formic acid during winter at a low concentration for extended periods significantly suppressed spore buildup of mixed populations of nosema (Nosema apis and Nosema ceranae) in 1 yr. PMID:19886435

  15. EM International. Volume 1

    1993-07-01

    It is the intent of EM International to describe the Office of Environmental Restoration and Waste Management`s (EM`s) various roles and responsibilities within the international community. Cooperative agreements and programs, descriptions of projects and technologies, and synopses of visits to international sites are all highlighted in this semiannual journal. Focus on EM programs in this issue is on international collaboration in vitrification projects. Technology highlights covers: in situ sealing for contaminated sites; and remote sensors for toxic pollutants. Section on profiles of countries includes: Arctic contamination by the former Soviet Union, and EM activities with Germany--cooperative arrangements.

  16. International EMS Systems

    Langhelle, Audun; Lossius, Hans Morten; Silfvast, Tom;

    2004-01-01

    . Access to on-line medical advice of a physician is not available; (2) Finland: the autonomy of the individual municipalities and their responsibility to cover for primary and specialised health care, as well as the EMS, and the lack of supporting or demanding legislation regarding the EMS; (3) Iceland is...... the only country that has emergency medicine (EM) as a recognised speciality but there is a need for more fully trained specialists in EM; (4) Norway: the ordinary ground ambulance is pointed out as the weakest link in the EM chain and a health reform demands extensive co-operation between the new...... health enterprises to re-establish a nation-wide air ambulance service; (5) Sweden: to create evidence based medicine standards for treatment in emergency medicine, a better integration of all part of the chain of survival, a formalised education in EM and a nation wide physician staffed helicopter EMS...

  17. First description of the nymph and larva of Dermacentor compactus Neumann, 1901 (Acari: Ixodidae), parasites of squirrels (Rodentia: Sciuridae) in southeast Asia.

    Apanaskevich, Dmitry A

    2016-05-01

    Recent reexamination of collection lots stored in the United States National Tick Collection revealed adult specimens of Dermacentor compactus Neumann, 1901 (Acari: Ixodidae) reared from field-collected nymphs, which allowed us to associate field-collected unidentified nymphs and larvae with this species. Nymphs of D. compactus can be easily distinguished from those of other congeneric species by the shape of the scutum and spiracular plate, the hypostome dentition, and the size of the spurs on the coxae. Larvae of this species can be distinguished by the shape and sculpture of the scutum, the shape of basis capituli, the absence of auriculae, and the size of the spurs on coxae II and III. Both nymphs and larvae feed mostly on various species of squirrels (Rodentia: Sciuridae). Considerably fewer nymphs and larvae were found on murid rodents (Rodentia: Muridae), domestic dogs (Carnivora: Canidae), and a snake (Squamata: Colubridae). PMID:27095664

  18. Ocorrência do ácaro vermelho europeu Panonychus ulmi (Koch (Tetranychidae associado à cultura da videira no Rio Grande do Sul, Brasil European red spider mite Panonychus ulmi (Koch (Tetranychidae occurrence of vineyards in Rio Grande do Sul, Brazil

    Noeli Juarez Ferla

    2008-09-01

    Full Text Available Neste artigo é descrita ocorrência e o dano causado pelo ácaro vermelho europeu Panonychus ulmi (Koch associado à cultura da videira. As coletas foram realizadas na safra 2005-2006 em Vitis vinifera L. da cultivar Merlot, nos municípios de Bento Gonçalves e Candiota, no Estado do Rio Grande do Sul, Brasil. As folhas infestadas apresentaram bronzeamento, com manchas avermelhadas na face adaxial, resultando na queda prematura das mesmas. Esse é o primeiro registro do ácaro vermelho europeu danificando a cultura da videira no Rio Grande do Sul.The occurrence and the damage caused for the European red spider mite Panonychus ulmi (Koch associated to the culture of the grapevine are described. The collections had been carried through in 2005-2006 seasons in Vitis vinifera L. of cultivating Merlot in Bento Gonçalves and Candiota counties, in the state of Rio Grande do Sul, Brazil. The infested leaves had presented bronzing, with spots redly in the adaxial face resulting in the premature fall. This is the first register of the European red mite damaging the culture of the grapevine in Rio Grande do Sul.

  19. International EMS Systems

    Langhelle, Audun; Lossius, Hans Morten; Silfvast, Tom;

    2004-01-01

    Emergency medicine service (EMS) systems in the five Nordic countries have more similarities than differences. One similarity is the involvement of anaesthesiologists as pre-hospital physicians and their strong participation for all critically ill and injured patients in-hospital. Discrepancies do....... Access to on-line medical advice of a physician is not available; (2) Finland: the autonomy of the individual municipalities and their responsibility to cover for primary and specialised health care, as well as the EMS, and the lack of supporting or demanding legislation regarding the EMS; (3) Iceland...... is the only country that has emergency medicine (EM) as a recognised speciality but there is a need for more fully trained specialists in EM; (4) Norway: the ordinary ground ambulance is pointed out as the weakest link in the EM chain and a health reform demands extensive co-operation between the new health...

  20. Isolation and Characterization of a Lycopene ε-Cyclase Gene of <em>Chlorella em>(Chromochloris> <em>zofingiensis>. Regulation of the Carotenogenic Pathway by Nitrogen and Light

    Maria Angeles Vargas

    2012-09-01

    Full Text Available The isolation and characterization of the lycopene ε-cyclase gene from the green microalga <em>Chlorella em>(Chromochloris> <em>zofingiensis> (<em>Czlcy-e> was performed. This gene is involved in the formation of the carotenoids α-carotene and lutein. <em>Czlcy-e> gene encoded a polypeptide of 654 amino acids. A single copy of <em>Czlcy-e> was found in <em>C. zofingiensisem>. Functional analysis by heterologous complementation in <em>Escherichia coliem> showed the ability of this protein to convert lycopene to δ-carotene. In addition, the regulation of the carotenogenic pathway by light and nitrogen was also studied in <em>C. zofingiensisem>. High irradiance stress did not increase mRNA levels of neither lycopene β<em>->cyclase gene (<em>lcy-b> nor lycopene ε-cyclase gene<em> em>(lcy-e> as compared with low irradiance conditions, whereas the transcript levels of <em>psy>, <em>pds>, <em>chyB> and <em>bkt> genes were enhanced, nevertheless triggering the synthesis of the secondary carotenoids astaxanthin, canthaxanthin and zeaxanthin and decreasing the levels of the primary carotenoids α-carotene, lutein, violaxanthin and β-carotene. Nitrogen starvation <em>per seem> enhanced mRNA levels of all genes considered, except <em>lcy-e and pdsem>, but did not trigger the synthesis of astaxanthin, canthaxanthin nor zeaxanthin. The combined effect of both high light and nitrogen starvation stresses enhanced significantly the accumulation of these carotenoids as well as the transcript levels of <em>bkt> gene, as compared with the effect of only high irradiance stress.

  1. Emergency Medical Service (EMS) Stations

    Kansas Data Access and Support Center — EMS Locations in Kansas The EMS stations dataset consists of any location where emergency medical services (EMS) personnel are stationed or based out of, or where...

  2. Biological and ecological characterization of two mites (Tetranychus Urticae and Phytoseiulus Persimilis) occurring in some agro-ecosystems; Caratterizzazione biologica ed ecologica di due acari (Tetranichus Urticae e Phytoseiulus Persimilis) interagenti in alcuni ecosistemi agrari

    Calvitti, M. [ENEA, Centro Ricerche Casaccia, Roma (Italy). Dip. Innovazione

    1995-12-01

    This work is a brief review of the actual knowledge about biological and ecological characteristics of two species of mites: Tetranychus urticae Koch (Acarina Tetranychidae) (two-spotted spider mite) and the predaceous mite Phytoseiulus persimilis Athias Henriot (Acarina Phytoseiidae). Success obtained in biological control of spider mite, by mass release of P. persimilis, has increased the interest in biological and ecological study of these mites. Particularly, the following biological and ecological aspects of both P. persimilis and T. urticae are hereby discussed: reproductive biology; population dynamics (spider mites outbreaks) and natural regulation of the trophic interaction; feeding behaviour; biological control of two-spotted spider mite by P. persimilis. In this report experimental data obtained in laboratory have been integrated with bibliographic information concerning studies produced in natural conditions.

  3. The <em>Opuntia streptacanthaem> <em>OpsHSP18 em>Gene Confers Salt and Osmotic Stress Tolerance in <em>Arabidopsis thalianaem>

    Juan Francisco Jiménez-Bremont

    2012-08-01

    Full Text Available Abiotic stress limits seed germination, plant growth, flowering and fruit quality, causing economic decrease. Small Heat Shock Proteins (sHSPs are chaperons with roles in stress tolerance. Herein, we report the functional characterization of a cytosolic class CI sHSP (OpsHSP18 from <em>Opuntia streptacantha em>during seed germination in <em>Arabidopsis thalianaem> transgenic lines subjected to different stress and hormone treatments. The over-expression of the <em>OpsHSP18em> gene in <em>A. thalianaem> increased the seed germination rate under salt (NaCl and osmotic (glucose and mannitol stress, and in ABA treatments, compared with WT. On the other hand, the over-expression of the <em>OpsHSP18em> gene enhanced tolerance to salt (150 mM NaCl and osmotic (274 mM mannitol stress in <em>Arabidopsis> seedlings treated during 14 and 21 days, respectively. These plants showed increased survival rates (52.00 and 73.33%, respectively with respect to the WT (18.75 and 53.75%, respectively. Thus, our results show that <em>OpsHSP18em> gene might have an important role in abiotic stress tolerance, in particular in seed germination and survival rate of Arabidopsis plants under unfavorable conditions.

  4. Caracterização de um vírus baciliforme isolado de Solanum violaefolium transmitido pelos ácaros Brevipalpus phoenicis e Brevipalpus obovatus (Acari: Tenuipalpidae Characterization of a bacilliform virus isolated from Solanum violaefolium transmitted by the tenuipalpid mites Brevipalpus phoenicis and Brevipalpus obovatus

    Paulo de Tarso Oliveira Ferreira

    2007-09-01

    Full Text Available Solano-violeta (Solanum violaefolium é uma planta ornamental rasteira usada para cobrir solos de áreas sombreadas. Um vírus que induz manchas anelares nas folhas desta planta, tentativamente designado Solanum violaefolium ringspot virus - SvRSV, transmitido pelo ácaro Brevipalpus phoenicis (Acari: Tenuipalpidae foi encontrado em Piracicaba, SP. Trata-se de um vírus baciliforme que se assemelha a outros vírus do tipo citoplasmático transmitidos por Brevipalpus sp. Este trabalho teve como objetivo relatar propriedades biológicas e estabelecer uma caracterização molecular parcial do SvRSV. O vírus pode ser transmitido mecanicamente a várias outras espécies botânicas, causando lesões localizadas. Entre as espécies avaliadas, Datura stramonium mostrou-se a melhor hospedeira experimental. Observou-se também a manifestação de sintomas nestas plantas após infestação das mesmas por B. obovatus previamente alimentado em lesões de SvRSV, confirmando esta outra espécie de ácaro como vetor do vírus. Suas propriedades físicas in vitro foram: temperatura de inativação 40-45 ºC; ponto final de diluição 10-3-10-4; longevidade in vitro 12 dias. Em secções ultrafinas, as partículas do SvRSV mostraram-se levemente mais delgadas e mais longas que as de outros vírus do mesmo grupo. A partir do dsRNA do SvRSV foi construída uma biblioteca de cDNA e foram identificadas duas possíveis regiões codificadoras das proteínas de movimento e replicase viral. Baseado nestas regiões foram desenhados "primers" para amplificação do RNA do SvRSV por RT-PCR. Sondas baseadas nas seqüências obtidas hibridizaram com ss- e dsRNA de D. stramonium infectadas pelo vírus. Ensaios preliminares de RT-PCR e hibridização não resultaram em reação com o vírus da leprose dos citros, tipo citoplasmático (CiLV-C.Solanum violaefolium is an ornamental plant, with prostrate, trailing growth habit and is cultivated in shaded areas. A virus that causes

  5. Expression of Selected <em>Ginkgo em>>biloba em>Heat Shock Protein Genes After Cold Treatment Could Be Induced by Other Abiotic Stress

    Feng Xu

    2012-05-01

    Full Text Available Heat shock proteins (HSPs play various stress-protective roles in plants. In this study, three <em>HSP> genes were isolated from a suppression subtractive hybridization (SSH cDNA library of <em>Ginkgo bilobaem> leaves treated with cold stress. Based on the molecular weight, the three genes were designated <em>GbHSP16.8em>, <em>GbHSP17em> and <em>GbHSP70em>. The full length of the three genes were predicted to encode three polypeptide chains containing 149 amino acids (Aa, 152 Aa, and 657 Aa, and their corresponding molecular weights were predicted as follows: 16.67 kDa, 17.39 kDa, and 71.81 kDa respectively. The three genes exhibited distinctive expression patterns in different organs or development stages. <em>GbHSP16.8em> and <em>GbHSP70em> showed high expression levels in leaves and a low level in gynoecia, <em>GbHSP17em> showed a higher transcription in stamens and lower level in fruit. This result indicates that <em>GbHSP16.8em> and <em>GbHSP70 em>may play important roles in <em>Ginkgo> leaf development and photosynthesis, and <em>GbHSP17em> may play a positive role in pollen maturation. All three <em>GbHSPs> were up-regulated under cold stress, whereas extreme heat stress only caused up-regulation of <em>GbHSP70em>, UV-B treatment resulted in up-regulation of <em>GbHSP16.8em> and <em>GbHSP17em>, wounding treatment resulted in up-regulation of <em>GbHSP16.8em> and <em>GbHSP70em>, and abscisic acid (ABA treatment caused up-regulation of <em>GbHSP70em> primarily.

  6. Osteoartrites em equinos

    Rocha, Francisco José Martins

    2008-01-01

    Dissertação de Mestrado Integrado em Medicina Veterinária A Osteoartrite (OA) é a principal causa de claudicação no cavalo de desporto e lazer, sendo uma afecção que tem grandes repercussões económicas. Este trabalho descreve algumas das características importantes da estrutura articular, bem como da sua fisiologia. Define a OA e todas as estruturas envolvidas no seu processo. Os mecanismos fisiopatológicos põem em evidência os factores de risco em causa e que determinam tod...

  7. A creche em Portugal

    Serrano, Leonor; Pinto, Jorge

    2015-01-01

    Na evolução das creches em Portugal verifica-se uma alternância entre conceções assistencialista de integração educacional. Procura-se refletir sobre a tensão existente entre estas duas perspetivas relativamente ao atendimento em creche em Portugal. A creche foi considerada como local de guarda por impedimento dos pais, no entanto no decurso dos tempos assistimos a tentativas de a assumir como um serviço de prestação de cuidados e de educação promovendo um desenvolvimento mais holístico e res...

  8. Homeopatia em Pediatria

    Pinto, Ana Isabel Alves

    2013-01-01

    Numa sociedade em constante processo de formação e de informação, não conhecer determinada terapia não pode ser a resposta de um profissional de farmácia ao seu utente. A divulgação massiva de tratamentos naturalistas, alternativos e baseados em saberes ancestrais, frequentemente entram em conflito com as prescrições médicas e aconselhamentos farmacêuticos. Com as medicinas alternativas a ganhar terreno paulatinamente, como se observa com a utilização da acupunctura e de fitote...

  9. Glycosylation of Vanillin and 8-Nordihydrocapsaicin by Cultured <em>Eucalyptus perrinianaem> Cells

    Naoji Kubota

    2012-05-01

    Full Text Available Glycosylation of vanilloids such as vanillin and 8-nordihydrocapsaicin by cultured plant cells of <em>Eucalyptus perrinianaem> was studied. Vanillin was converted into vanillin 4-<em>O>-b-D-glucopyranoside, vanillyl alcohol, and 4-<em>O>-b-D-glucopyranosylvanillyl alcohol by <em>E. perriniana em>cells. Incubation of cultured <em>E. perrinianaem> cells with 8-nor- dihydrocapsaicin gave 8-nordihydrocapsaicin 4-<em>O>-b-D-glucopyranoside and 8-nordihydro- capsaicin 4-<em>O>-b-D-gentiobioside.

  10. Study of the <em>in Vitroem> Antiplasmodial, Antileishmanial and Antitrypanosomal Activities of Medicinal Plants from Saudi Arabia

    Nawal M. Al-Musayeib

    2012-09-01

    Full Text Available The present study investigated the <em>in vitroem> antiprotozoal activity of sixteen selected medicinal plants. Plant materials were extracted with methanol and screened <em>in vitroem> against erythrocytic schizonts of <em>Plasmodium falciparumem>, intracellular amastigotes of <em>Leishmania infantum em>and <em>Trypanosoma cruzi em>and free trypomastigotes of<em> T. bruceiem>. Cytotoxic activity was determined against MRC-5 cells to assess selectivity<em>. em>The criterion for activity was an IC50 < 10 µg/mL (4. Antiplasmodial activity was found in the<em> em>extracts of<em> em>>Prosopis julifloraem> and <em>Punica granatumem>. Antileishmanial activity<em> em>against <em>L. infantumem> was demonstrated in <em>Caralluma sinaicaem> and <em>Periploca aphylla.em> Amastigotes of<em> T. cruzi em>were affected by the methanol extract of<em> em>>Albizia lebbeckem>> em>pericarp, <em>Caralluma sinaicaem>,> Periploca aphylla em>and <em>Prosopius julifloraem>. Activity against<em> T. brucei em>was obtained in<em> em>>Prosopis julifloraem>. Cytotoxicity (MRC-5 IC50 < 10 µg/mL and hence non-specific activities were observed for<em> em>>Conocarpus lancifoliusem>.>

  11. Purification, Characterization and Antioxidant Activities <em>in Vitroem>> em>and <em>in Vivoem> of the Polysaccharides from <em>Boletus edulisem> Bull

    Yijun Fan

    2012-07-01

    Full Text Available A water-soluble polysaccharide (BEBP was extracted from <em>Boletus edulis em>Bull using hot water extraction followed by ethanol precipitation. The polysaccharide BEBP was further purified by chromatography on a DEAE-cellulose column, giving three major polysaccharide fractions termed BEBP-1, BEBP-2 and BEBP-3. In the next experiment, the average molecular weight (Mw, IR and monosaccharide compositional analysis of the three polysaccharide fractions were determined. The evaluation of antioxidant activities both <em>in vitroem> and <em>in vivo em>suggested that BEBP-3 had good potential antioxidant activity, and should be explored as a novel potential antioxidant.

  12. A specimen of <em>Sorex> cfr. <em>samniticus> in Barn Owl's pellets from Murge plateau (Apulia, Italy / Su di un <em>Sorex> cfr. <em>samniticus> (Insectivora, Soricidae rinvenuto in borre di <em>Tyto albaem> delle Murge (Puglia, Italia

    Giovanni Ferrara

    1992-07-01

    Full Text Available Abstract In a lot of Barn Owl's pellets from the Murge plateau a specimen of <em>Sorex> sp. was detected. Thank to some morphological and morphometrical features, the cranial bones can be tentatively attributed to <em>Sorex samniticusem> Altobello, 1926. The genus <em>Sorex> was not yet included in the Apulia's fauna southwards of the Gargano district; the origin and significance of the above record is briefly discussed, the actual presence of a natural population of <em>Sorex> in the Murge being not yet proved. Riassunto Viene segnalato il rinvenimento di un esemplare di <em>Sorex> cfr. <em>samniticus> da borre di <em>Tyto albaem> delle Murge. Poiché il genere non era stato ancora segnalato nella Puglia a sud del Gargano, viene discusso il significato faunistico del reperto.

  13. Repellency of oils of lemon eucalyptus, geranium, and lavender and the mosquito repellent MyggA natural to Ixodes ricinus (Acari: Ixodidae) in the laboratory and field.

    Jaenson, Thomas G T; Garboui, Samira; Palsson, Katinka

    2006-07-01

    MyggA Natural (Bioglan, Lund, Sweden) is a commercially available repellent against blood-feeding arthropods. It contains 30% of lemon-scented eucalyptus, Corymbia citriodora (Hook.) K. D. Hill & L. A. S. Johnson (Myrtaceae), oil with a minimum of 50% p-menthane-3,8-diol. MyggA Natural also contains small amounts of the essential oils of lavender, Lavandula angustifolia Mill. (Lamiaceae), and geranium, Pelargonium graveolens L'Her. (Geraniaceae). In laboratory bioassays, MyggA Natural and C. citriodora oil exhibited 100% repellency against host-seeking nymphs of Ixodes ricinus (L.) (Acari: Ixodidae). Lavender oil and geranium oil, when diluted to 1% in 1,2-propanediol, had weak repellent activities on I. ricinus nymphs, but when diluted to 30% in 1,2-propanediol had 100% repellencies. 1,2-Propanediol (100%) had no significant repellent activity in comparison with that of the control. In field tests in tick-infested areas in central Sweden, tick repellency of MyggA Natural and C. citriodora oil was tested by the blanket-dragging technique for 4 d during a 6-d period. The repellencies (74 and 85%, respectively) on day 1 are similar (89%) to that of blankets treated in a similar manner with 19% diethyl-methyl-benzamide, based on previous work. Repellencies declined significantly from day 1 to day 6 (74 to 45% for MyggA Natural; 85 to 42% for C. citriodora oil). PMID:16892632

  14. Acaricidal properties of vetiver essential oil from Chrysopogon zizanioides (Poaceae) against the tick species Amblyomma cajennense and Rhipicephalus (Boophilus) microplus (Acari: Ixodidae).

    Campos, Roseane Nunes de Santana; Nascimento Lima, Cecília Beatriz; Passos Oliveira, Alexandre; Albano Araújo, Ana Paula; Fitzgerald Blank, Arie; Barreto Alves, Péricles; Nascimento Lima, Rafaely; Albano Araújo, Vinícius; Santana, Alisson Silva; Bacci, Leandro

    2015-09-15

    Ticks are arthropods widely distributed in tropical and subtropical regions, which can transmit infectious agents also responsible for zoonoses. Excessive use of conventional acaricides has resulted in the onset of drug resistance by these parasites, thus the need to use alternative methods for their control. This study evaluated the acaricidal activities of Chrysopogon zizanioides (vetiver) essential oils containing different zizanoic and khuzimol (high and low acidity) acid concentrations on Amblyomma cajennense and Rhipicephalus microplus (Acari: Ixodidae). To this aims, toxicity tests of different concentrations of examined essential oils were conducted on adult females and larval stages. Results showed that the essential oils of C. zizanioides with high and low acidity reduced oviposition of females, eggs hatch and larval survival, being more effective than some commercial products widely used to control these ectoparasites. These results indicate that the C. zizanoides essential oils are promising candidates as acaricidal agents and represent also an add value to vetiver oil with high acidity, which is commercially undervalued in the cosmetic industry. PMID:26359641

  15. First Record of Amblyomma ovale (Koch, 1844 (Acari: Ixodidae parasitizing Lontra longicaudis (Olfers, 1818 (Carnivora: Mustelidae in Santa Catarina Island, Florianópolis, Sc, Brazil

    Bibiana Verdin De Andrade

    2015-04-01

    Full Text Available Three female and one male Amblyomma ovale (Acari: Ixodidae were found parasitizing a neotropical otter (Lontra longicaudis (Carnivora: Mustelidae in Florianópolis, SC, Brazil. This is the first record of the occurrence of A. ovale in Santa Catarina Island and the second report of this ectoparasite in a mustelid species in Brazil. A. ovale has been reported in wild carnivores in several Brazilian states and is considered one of the main predominant species of the Atlantic rainforest. The Amblyomma ticks are primarily responsible for the spread of BSF in humans. Among the species of Amblyomma infesting dogs is the A. ovale and the proximity between man and the dog favors the parasite sharing. Santa Catarina Island has many rural and wild environments shared by domestic animals, especially dogs, such as the environment where the otter was found in this study. Proper determination of tick species and their distribution in different geographical regions is essential for the understanding of the epidemiology of Rickettsiosis.

  16. <em>In Vivoem> Histamine Optical Nanosensors

    Heather A. Clark

    2012-08-01

    Full Text Available In this communication we discuss the development of ionophore based nanosensors for the detection and monitoring of histamine levels <em>in vivoem>. This approach is based on the use of an amine-reactive, broad spectrum ionophore which is capable of recognizing and binding to histamine. We pair this ionophore with our already established nanosensor platform, and demonstrate <em>in vitroem> and <em>in vivoem> monitoring of histamine levels. This approach enables capturing rapid kinetics of histamine after injection, which are more difficult to measure with standard approaches such as blood sampling, especially on small research models. The coupling together of <em>in vivoem> nanosensors with ionophores such as nonactin provide a way to generate nanosensors for novel targets without the difficult process of designing and synthesizing novel ionophores.

  17. Parasitic zoonoses: survey in foxes (<em>Vulpes vulpesem>) in the northern Apennines / Zoonosi parassitarie: indagini in volpi (<em>Vulpes vulpesem>) dell'Appennino settentrionale

    Vittorio Guberti; Giovanni Poglayen

    1991-01-01

    Abstract A parasitological survey on 153 foxes was carried out in the northern Apennines, during the period 1984-1987. The following parasites were identified: <em>Toxocara canisem> (46.4%), <em>Taenia> sp. (17%), <em>Uncinaria stenocephalaem> (11.8%), <em>Mesocestoides lineatusem> (11.1%), <em>Ancylostoma caninumem> (3.9%), <em>Taenia hydatigenaem> (3.3%), <em>Trichuris v...

  18. Burnout em cuidados paliativos

    Simões, Sílvia Cristina Almeida

    2013-01-01

    Dissertação apresentada ao Instituto Politécnico de Castelo Branco para cumprimento dos requisitos necessário à obtenção de grau de Mestre em Cuidados Paliativos. O presente trabalho foi realizado no âmbito do 3º semestre do 1º Curso de Mestrado em Cuidados Paliativos, da Escola Superior de Saúde Dr. Lopes Dias. O trabalho encontra-se estruturado em quatro capítulos: o primeiro diz respeito ao relatório da prática clínica, o segundo aborda o enquadramento teórico sobre a Síndrome de Bur...

  19. Evaluation of Pseudopteroxazole and Pseudopterosin Derivatives against <em>Mycobacterium> <em>tuberculosis> and Other Pathogens

    McCulloch, Malcolm W. B.; Brad Haltli; Marchbank, Douglas H.; Kerr, Russell G.

    2012-01-01

    Pseudopterosins and pseudopteroxazole are intriguing marine natural products that possess notable antimicrobial activity with a commensurate lack of cytotoxicity. New semi-synthetic pseudopteroxazoles, pseudopteroquinoxalines and pseudopterosin congeners along with simple synthetic mimics of the terpene skeleton were synthesized. In order to build structure-activity relationships, a set of 29 new and previously reported compounds was assessed for <em>in> <em>vitro...

  20. Ulisses em Kafka

    Stéphane Mosès

    2015-02-01

    Full Text Available ResumoEm sua introdução para Exegese de uma lenda, Stéphane Mosès afirma que a obra de Kafka não necessita de novas interpretações, mas sim de uma “análise rigorosa de sua lógica subjacente”. Assim, em “Ulisses em Kafka”, investigandoa dialética formal de “O silêncio das sereias”, Mosès procura expor a estrutura narrativa que comandaria esse breve texto em que o escritor tcheco acrescenta à astucia de Ulisses a ingenuidade como elemento de salvação.Palavras-chave: Kafka; dialética; salvação.AbstractIn his introduction to Exegèse d’une légende, Stéphane Mosès affirms that Kafka’s works are not in need of new interpretations, but rather, of a “rigorous analysis of their underlying logic”. Thus, in “Ulisses chez Kafka”, by investigating the formal dialectic of “Das Schweigen der Sirenen”, Mosès seeks to exhibit the narrative structure that would supposedly guide this short text in which the Czech author adds naïveté to Ulisses’s cunning intelligence as an element of salvation.Keywords: Kafka; dialectic; salvation.Stéphane Mosès foi professor emérito da Universidade Hebraica de Jerusalém, onde ensinou Literatura Alemã e Comparada, entre os seus principais interesses estavam o pensamento de Franz Rosenzweig e de Walter Benjamin e as literaturas de Franz Kafka e de Paul Célan. Autor de diversos livros, entre eles: L'ange de l'histoire. Rosenzweig, Benjamin, Scholem, Exégèse d'une légende, lectures de Kafka, e Rêves de Freud. Six lectures.Rodrigo Ielpo é doutor em literatura francesa pela UFRJ e em História e Semiologia do texto e da imagem pela Université Paris VII, com pós-doutorado em Teoria Literária pela UNICAMP. Professor Adjunto do Departamento de Letras Neolatinas da Universidade Federal do Rio de Janeiro, possui publicações e traduções nos seguintes temas: teoria e literatura francesa contemporâneas, escrita e processos de subjetivação.rodrigoielpo@gmail.com 

  1. Horizontal Transmission of the Entomopathogen Fungus <em>Metarhizium anisopliae em>in <em>Microcerotermes diversusem> Groups

    Mohammad Saied Mossadegh

    2012-08-01

    Full Text Available An experiment was carried out in order to investigate fungal conidia transmission of <em>Metarhizium anisopliae em>(Metschnikoff> em>Sorokin from vector (donor to healthy <em>Microcerotermes diversusem> Silvestri (Iso.: Termitidae and determine the best donor/concentration ratio for transmission. After preliminary trials, concentrations of 3.1 × 104, 3.9 × 105, 3.2 × 106 and 3.5 × 108 conidia mL−1 were selected for testing. The experiment was performed at three donor : Recipient ratios of 10, 30 and 50%. The highest mortality of recipient workers was observed after 14 days at the concentration of 3.5 × 108 conidia mL−1 and donor ratio of 50%. The mortality of recipient workers was less than 20% at all concentrations at a donor ratio of 10%. Our observations indicate social behavior of <em>M. diversusem>, such as grooming, can be effective in promoting epizootic outbreaks in a colony. While the current results suggest good potential for efficacy, the use of <em>M. anisopliaeem> as a component of integrated pest management of <em>M. diversusem> still needs to be proven under field conditions.

  2. Fumigant Antifungal Activity of Myrtaceae Essential Oils and Constituents from <em>Leptospermum petersoniiem> against Three <em>Aspergillus> Species

    Il-Kwon Park

    2012-09-01

    Full Text Available Commercial plant essential oils obtained from 11 Myrtaceae plant species were tested for their fumigant antifungal activity against <em>Aspergillus ochraceusem>, <em>A. flavusem>, and <em>A. nigerem>. Essential oils extracted from<em> em>Leptospermum> <em>petersonii> at air concentrations of 56 × 10−3 mg/mL and 28 × 10−3 mg/mL completely inhibited the growth of the three <em>Aspergillus> species. However, at an air concentration of 14 × 10−3 mg/mL, inhibition rates of <em>L. petersoniiem> essential oils were reduced to 20.2% and 18.8% in the case of <em>A. flavusem> and <em>A. nigerem>, respectively. The other Myrtaceae essential oils (56 × 10−3 mg/mL only weakly inhibited the fungi or had no detectable affect. Gas chromatography-mass spectrometry analysis identified 16 compounds in <em>L. petersoniiem>> em>essential> em>oil.> em>The antifungal activity of the identified compounds was tested individually by using standard or synthesized compounds. Of these, neral and geranial inhibited growth by 100%, at an air concentration of 56 × 10−3 mg/mL, whereas the activity of citronellol was somewhat lover (80%. The other compounds exhibited only moderate or weak antifungal activity. The antifungal activities of blends of constituents identified in <em>L. petersoniiem> oil indicated that neral and geranial were the major contributors to the fumigant and antifungal activities.

  3. Atividade in vitro de permetrina, cipermetrina e deltametrina sobre larvas de Rhipicephalus sanguineus (Latreille, 1806 (Acari, Ixodidae In vitro activity of permethrin, cipermethrin and deltamethrin on larvae of Rhipicephalus sanguineus (Latreille, 1806 (Acari, Ixodidae

    F.F. Fernandes

    2000-12-01

    Full Text Available Investigaram-se os efeitos toxicológicos in vitro de três piretróides em diferentes concentrações, formulados para uso pecuário, domiciliar e em cães, sobre larvas de R. sanguineus, objetivando monitorar sua suscetibilidade e fomentar seu controle. Utilizaram-se 1.604 larvas em jejum, com 15 a 21 dias, obtidas por infestação artificial em cão, imersas nas soluções testadas, contidas em dispositivos constituídos por placa de Petri descartável, papel filtro e parafina, mantidas em incubadoras do tipo BOD e observadas ao estereoscópio por 24h. Observaram-se excitabilidade, movimentação repetitiva, diminuição da locomoção, desprendimento, paralisia, "knock-down" e proliferação cuticular de gases e líquidos. A mortalidade na 24ª hora foi de 86,9%, 100,0%, 80,3%, 86,0%, 68,2% e 78,0%, respectivamente, para permetrina 1250ppm e 2500ppm, cipermetrina 150ppm e 300ppm, e deltametrina 25ppm e 50ppm. Não houve mortalidade no grupo controle.Aiming at monitoring the susceptibility, increasing the control and evaluating the toxicological effects, of different concentrations of three pyrethroids were investigated in vitro against Rhipicephalus sanguineus larvae using concentrations formulated for cattle, dogs and household use. A total of 1,604 15-21 day-old fasting larvae, obtained by artificial infestation in dogs, was immersed in the solutions. Larvae were then maintained in BOD incubators inside experimental devices, constituted by disposable Petridishes, filter paper and paraffin, and observed on the stereomicroscope during a 24-hour period. The larvae have shown excitability, repetitive movements, decrease of locomotion, detachment, paralysis, knock-down and production of gases and liquid in the cuticle. Mortality after 24 hours was 86.9% (1250ppm and 100.0% (2500ppm for permethrin; 80.3% (150ppm and 86.0% (300ppm for cipermethrin; and for deltamethrin 68.2% (25ppm and 78.0% (50ppm. Mortality was not observed in the control group.

  4. Espondiloptose em atleta

    Ana Paula Luppino Assad

    2014-06-01

    Full Text Available Os atletas adolescentes estão sob maior risco de lombalgia e lesões estruturais da coluna. A espondilólise é responsável pela maioria das lombalgias em jovens esportistas e raramente ocorre em adultos. Relatamos o caso de uma paciente de 13 anos, atleta de judô, que chegou a nosso serviço com quadro de cinco meses de lombalgia progressiva durante os treinos, sendo inicialmente atribuída a causas mecânicas, sem que houvesse uma investigação mais detalhada por métodos de imagem. Na admissão já apresentava deformidade lombar, postura antálgica e manobra de hiperextensão lombar em unipodálico positiva bilateralmente. Realizou-se investigação, que evidenciou espondiloptose, sendo, então, submetida a tratamento cirúrgico. Com base neste relato de caso, discutimos a abordagem diagnóstica de lombalgia em atletas jovens, uma vez que a queixa de lombalgia crônica pode ser marcador de uma lesão estrutural, a qual pode ser definitiva e trazer perda funcional irreversível.

  5. First record of Parakosa flexipes (Acari: Chirodiscidae parasitizing a free-tailed bat (Chiroptera: Molossidae in Brazil Primeiro registro de Parakosa flexipes (Acari: Chirodiscidae parasitando um morcego-de-cauda-livre (Chiroptera: Molossidae no Brasil

    Luiz Antonio Costa Gomes

    2012-12-01

    Full Text Available The present study reports the occurrence of Parakosa flexipes (Pinichpongse (Chirodiscidae for the first time in Brazil, along with its infection sites on Molossus rufus E. Geoffroy. Thirty-eight bats were caught using mist nets that were placed near a house at the Mars Center for Cocoa Science in the state of Bahia, of which 14 (37% were parasitized by P. flexipes. Parakosa flexipes was observed parasitizing M. rufus on hairs that were evidently longer than others distributed over the bat's body, where up to three parasites could be spotted on a single hair.O presente estudo registra a ocorrência de Parakosa flexipes (Pinichpongse pela primeira vez no Brasil, bem como seus sítios de infecção em Molossus rufus E. Geoffroy. Foram capturados 38 morcegos com redes-de-neblina, dispostas próximas a uma residência da "Mars Center for Cocoa Science" no Sul da Bahia, dos quais 14 (37% estavam parasitados por P. flexipes. Parakosa flexipes foi observada parasitando M. rufus em pelos evidentemente maiores que os demais distribuídos pelo corpo do morcego, onde se pôde observar até três parasitos em um único pelo.

  6. Influence of long-term exposure to simulated acid rain on development, reproduction and acaricide susceptibility of the carmine spider mite, Tetranychus cinnabarinus

    Wang, Jin-Jun; Zhang, Jian-ping; He, Lin; Zhao, Zhi-Mo

    2006-01-01

    Development, reproduction and acaricide susceptibility of Tetranychus cinnabarinus (Boisduvals) (Acari: Tetranychidae) were investigated after long-term (about 40 generations) exposure to various levels of acid rain; pH 2.5, 3.0, 4.0, and 5.6. Deionized water (pH 6.8) served as a control. The mites were reared on eggplant leaves at 28°C, 80%RH and a photoperiod of 14:10 (L:D) in the laboratory. The results showed that the duration of the immature stage was significantly affected by acid rain ...

  7. Ultrasound-Assisted Extraction of Syringin from the Bark of <em>Ilex em>rotunda> Thumb Using Response Surface Methodology

    Hui Wang

    2012-06-01

    Full Text Available In this work, a rapid extraction method based on ultrasound-assisted extraction (UAE of syringin from the bark of <em>Ilex rotunda em>Thumb using response surface methodology (RSM is described. The syringin was analyzed and quantified by high performance liquid chromatography coupled with UV detection (HPLC-UV. The extraction solvent, extraction temperature and extraction time, the three main factors for UAE, were optimized with Box-Behnken design (BBD to obtain the highest extraction efficiency. The optimal conditions were the use of a sonication frequency of 40 kHz, 65% methanol as the solvent, an extraction time of 30 min and an extraction temperature of 40 °C. Using these optimal conditions, the experimental values agreed closely with the predicted values. The analysis of variance (ANOVA indicated a high goodness of model fit and the success of the RSM method for optimizing syringin extraction from the bark of <em>I>. em>rotunda>.

  8. Classe social: conceitos e esquemas operacionais em pesquisa em saude

    Rita Barradas Barata

    2013-08-01

    Full Text Available Discute-se a utilização do conceito de classe em pesquisas em saúde, as diferentes abordagens sociológicas de estratificação social e de estrutura de classes, o potencial explicativo do conceito em estudos de determinação social e desigualdades em saúde, os modelos de operacionalização elaborados para uso em pesquisas sociológicas, demográficas ou de saúde e os limites e possibilidades desses modelos. Foram destacados quatro modelos de operacionalização: de Singer para estudo da distribuição de renda no Brasil, adaptado por Barros para uso em pesquisas epidemiológicas; de Bronfman & Tuirán para o censo demográfico mexicano, adaptado por Lombardi et al para pesquisas epidemiológicas; de Goldthorpe para estudos socioeconômicos ingleses, adaptado pela Sociedade Espanhola de Epidemiologia; e o modelo de Wright para pesquisa em sociologia e ciência política, também usado em inquéritos populacionais em saúde. Em conclusão, conceitualmente cada um dos modelos apresentados é coerente com a concepção teórica que os embasam, mas não há como optar por qualquer deles, descartando os demais.

  9. Iodo em alimentos consumidos em Portugal

    Coelho, Inês; Delgado, Inês; Costa, Sofia; Castanheira, Isabel; Calhau, Maria Antónia

    2015-01-01

    O iodo é um elemento vestigial essencial na dieta humana e animal, com uma importância nutricional bem estabelecida. É indispensável para a síntese das hormonas da tiroide, tiroxina e triiodotirosina, cujo principal papel está relacionado com o crescimento e desenvolvimento dos órgãos, em particular do cérebro. A fonte natural de iodo são os alimentos. Contudo, de acordo com a OMS um terço da população mundial sofre de algum tipo de carência de iodo. A deficiência crônica de iodo pode levar a...

  10. Prima segnalazione di <em>Brachylaima fulvusem> Dujardin, 1843 (Digenea, Brachylaimidae in <em>Sorex> cfr. <em>antinorii> Bonaparte, 1848 (Insectivora, Soricidae in Italia

    Juan Carlos Casanova

    2003-10-01

    Full Text Available Un?indagine parassitologica è stata condotta su esemplari di <em>Sorex> cfr. <em>antinorii> Bonaparte, 1840 (Insectivora, Soricidae catturati in Sila Grande (Valle Capra; 16.29.30; 39.21.40. Uno degli esemplari è stato trovato parassitato da Trematodi digenei rinvenuti nell?esofago e nello stomaco. I parassiti isolati sono stati fissati con liquido di Bouin, colorati con carminio acetico di Semichon, fissati in una serie di alcooli, chiarificati in xilolo e montati con Balsamo del Canada. I parassiti sono stati identificati come membri della Famiglia Brachylaimidae Joyeux & Foley (1930 appartenenti al genere <em>Brachylaima> Dujardin (1843. La morfologia generale ed i caratteri metrici hanno permesso di identificarli come <em>B. fulvusem> Dujardin, 1843 (lunghezza corpo: 2.39-2.46; larghezza corpo: 0.72-0.78; diametro trasversale ventosa orale: 212.48- 238.08x240.64-266.24; ventosa ventrale: 250.88-258.56x250.88-266.24; faringe 120.32-168.96; testicoli: 151.04-189.44x168.96-189.44; ovario: 122.88x171.52; uova: 26-30x2-17. Il range dei dati morfometrici coincide con quelli riscontrati da diversi autori negli esemplari di <em>B. fulvusem> in Europa centrale e occidentale (Jourdane, 1971; Lewis, 1969; Zarnowski,1960; Mas-Coma & Gallego, 1975. Dopo la descrizione originale, la sistematica di <em>B. fulvusem> Blanchard, 1847 non è stata mai confutata, sebbene varie specie dei generi <em>Harmostomum> Braun, 1899 e <em>Panopistus Sinitzinem>, 1931 siano state proposte come sinonimi di <em>B. fulvusem> da diversi autori. Zarnowski (1960 considera aperta la questione di identità di <em>H. (H. dujardiniem> Baer, 1928 e propone la sinonimia di <em>B. oesophageiem> Shaldibin, 1953 e <em>B. fulvusem>. Lewis (1969, al contrario, ha riconvalidato <em>B. oesophageiem> come specie, utilizzando criteri che, però, Jourdane (1971 ritiene, non idonei a differenziare le due specie. Mas-Coma & Gallego (1975, considerano <em

  11. RT-PCR DETECTION OF CITRUS LEPROSIS VIRUS IN SHRUBS AND WEED PLANTS INFECTED THROUGH Brevipalpus phoenicis (ACARI: TENUIPALPIDAE)

    No Brasil são gastos anualmente cerca de US$ 80 milhões para controle de Brevipalpus phoenicis, vetor do vírus leprose dos citros (CiLV). Este ácaro possui comportamento polífago e cosmopolita, presente em inúmeras espécies de plantas junto aos pomares cítricos. Neste trabalho foi investigada a poss...

  12. A sustained release gel formulation of doramectin for control of lone star ticks (Acari: Ixodidae) and horn flies (Diptera: Muscidae) on cattle.

    Lohmeyer, K H; Miller, J A; Pound, J M; Klavons, J A

    2009-04-01

    A gel formulation formed by incorporating technical doramectin into a 10% hydroxypropyl methylcellulose aqueous solution was used to subcutaneously inject steers at varying dosages. Doramectin serum concentration of steers receiving 600 microg (AI)/kg body weight declined from 21.9 ppb at 0.5 wk to below detectable at 8 wk postinjection. The 1,200 microg (AI)/kg injection resulted in serum concentrations of 29.1 ppb at 0.5 wk and declined to 0.5 ppb at 8 wk postinjection. Both the 600 and 1,200 microg (AI)/kg injections provided 100% inhibition of index of fecundity (IF) in adult lone star ticks, Amblyomma americanum L. (Acari: Ixodidae) through week 8, after which inhibition declined to 79.4 and 45.3%, respectively, during the 12th week posttreatment. For steers treated at 600 microg (AI)/kg, mortality of adult horn flies, Hematobia irritans L. (Diptera: Muscidae), declined from 16.9% during week 2 to 3.1% during week 7 postinjection. The blood from steers treated at 1,200 microg (AI)/kg resulted in a similar decline in mortality of blood fed adult horn flies from 29.4% during week 1 to 4.0% during week 7. The 600 microg (AI)/kg treatment provided complete control of larval horn flies in the manure for 9 wk, whereas the 1,200 microg (AI)/kg injection gave complete control for 14 wk posttreatment. The doramectin gel formulation provided long-lasting delivery of doramectin to cattle and extended control of lone star ticks and larval horn flies. Such a simple and inexpensive formulation could be useful in tick eradication programs by reducing the frequency of gathering cattle. PMID:19449664

  13. Suppression of host-seeking Ixodes scapularis and Amblyomma americanum (Acari: Ixodidae) nymphs after dual applications of plant-derived acaricides in New Jersey.

    Jordan, Robert A; Dolan, Marc C; Piesman, Joseph; Schulze, Terry L

    2011-04-01

    We evaluated the ability of dual applications of natural, plant-derived acaricides to suppress nymphal Ixodes scapularis Say and Amblyomma americanum (L.) (Acari: Ixodidae) in a Lyme disease endemic area of New Jersey. An aqueous formulation of 2% nootkatone provided >90% control of I. scapularis through 7 d. Control declined to 80.9% at 14 d, and a second application was made that provided >95% control through the remaining 4 wk of the nymphal season. Nootkatone provided >90% control of A. americanum through 35 d postapplication. Applications of 2% carvacrol and EcoTrol T&O resulted in rapid knockdown of both tick species, but control declined significantly to 76.7 and 73.7%, respectively, after 14 d when a second application was made that extended control of both tick species to between 86.2 and 94.8% at 21 d. Subsequently, control declined steadily in all plots by 42 d postapplication except for I. scapularis in carvacrol-treated plots, where levels of control >90% were observed through 35 d. Of the three compounds tested, 2% nootkatone provided the most consistent results, with 96.5 and 91.9% control of I. scapularis and A. americanum through 42 and 35 d, respectively. The ability of plant-derived natural products to quickly suppress and maintain significant control of populations of these medically important ticks may represent a future alternative to the use of conventional synthetic acaricides. In addition, the demonstrated efficacy of properly-timed backpack sprayer application may enable homeowner access to these minimal-risk acaricides. PMID:21510219

  14. A review of the ticks (Acari, Ixodida of Brazil, their hosts and geographic distribution - 1. The State of Rio Grande do Sul, Southern Brazil

    Evans DE

    2000-01-01

    Full Text Available A review of the ticks (Acari, Ixodida of the State of Rio Grande do Sul, southern Brazil, was completed as a step towards a definitive list (currently indicated as 12 of such species, their hosts and distribution. The ticks: Argas miniatus (poultry, Ixodes loricatus (opossums, Amblyomma aureolatum (dogs, A. calcaratum (anteaters, A. cooperi (capybaras, A. nodosum (anteaters, A. tigrinum (dogs (Neotropical and Rhipicephalus sanguineus (dogs (introduced, cosmopolitan, Afrotropical were confirmed as present, in addition to the predominant, Boophilus microplus (cattle (introduced, pan-tropical, Oriental. Of the further 18 species thus far reported in the literature as present in the state, but unavailable for examination: only Ornithodoros brasiliensis (humans and their habitations (Neotropical, Ixodes affinis (deer (Nearctic/Neotropical and I. auritulus (birds (Nearctic/Neotropical/Afrotropical/ Australasian are considered likely; 13 species would benefit from corroborative local data but the majority appear unlikely; reports of A. maculatum (Nearctic/Neotropical, but circum-Caribbean are considered erroneous; the validity of A. fuscum is in doubt. The very recent, first known report of the tropical Anocentor nitens (horses(Nearctic/Neotropical, but still apparent absence of the tropical A. cajennense (catholic (Nearctic/Neotropical and the sub-tropical/temperate Ixodes pararicinus (cattle (Neotropical in Rio Grande do Sul are important for considerations on their current biogeographical distribution and its dynamics in South America. The state has relatively long established, introduced ("exotic", Old World tick species (B. microplus, R. sanguineus that continue to represent significant pests and disease vectors to their traditional, introduced domestic animal hosts, cattle and urban dogs. There are also indigenous, New World ticks (A. miniatus, O. brasiliensis, A. aureolatum, A. nitens, as both long established and possibly newly locally

  15. The EM Earthquake Precursor

    Jones, K. B., II; Saxton, P. T.

    2013-12-01

    Many attempts have been made to determine a sound forecasting method regarding earthquakes and warn the public in turn. Presently, the animal kingdom leads the precursor list alluding to a transmission related source. By applying the animal-based model to an electromagnetic (EM) wave model, various hypotheses were formed, but the most interesting one required the use of a magnetometer with a differing design and geometry. To date, numerous, high-end magnetometers have been in use in close proximity to fault zones for potential earthquake forecasting; however, something is still amiss. The problem still resides with what exactly is forecastable and the investigating direction of EM. After the 1989 Loma Prieta Earthquake, American earthquake investigators predetermined magnetometer use and a minimum earthquake magnitude necessary for EM detection. This action was set in motion, due to the extensive damage incurred and public outrage concerning earthquake forecasting; however, the magnetometers employed, grounded or buried, are completely subject to static and electric fields and have yet to correlate to an identifiable precursor. Secondly, there is neither a networked array for finding any epicentral locations, nor have there been any attempts to find even one. This methodology needs dismissal, because it is overly complicated, subject to continuous change, and provides no response time. As for the minimum magnitude threshold, which was set at M5, this is simply higher than what modern technological advances have gained. Detection can now be achieved at approximately M1, which greatly improves forecasting chances. A propagating precursor has now been detected in both the field and laboratory. Field antenna testing conducted outside the NE Texas town of Timpson in February, 2013, detected three strong EM sources along with numerous weaker signals. The antenna had mobility, and observations were noted for recurrence, duration, and frequency response. Next, two

  16. <em>Bacillus anthracisem> Factors for Phagosomal Escape

    Irene Zornetta

    2012-07-01

    Full Text Available The mechanism of phagosome escape by intracellular pathogens is an important step in the infectious cycle. During the establishment of anthrax, <em>Bacillus anthracisem> undergoes a transient intracellular phase in which spores are engulfed by local phagocytes. Spores germinate inside phagosomes and grow to vegetative bacilli, which emerge from their resident intracellular compartments, replicate and eventually exit from the plasma membrane. During germination, <em>B. anthracisem> secretes multiple factors that can help its resistance to the phagocytes. Here the possible role of <em>B. anthracisem> toxins, phospholipases, antioxidant enzymes and capsules in the phagosomal escape and survival, is analyzed and compared with that of factors of other microbial pathogens involved in the same type of process.

  17. On subspecific taxonomy of <em>Microtus saviiem> (Rodentia, Arvicolidae

    Longino Contoli

    2003-10-01

    Full Text Available Riassunto Sulla tassonomia sottospecifica di <em>Microtus saviiem> (Rodentia, Arvicolidae Viene riveduta e riassunta la situazione tassonomica sottospecifica di <em>Microtus (Terricola saviiem>, anche tramite la descrizione di due nuovi taxa: <em>Microtus (Terricola savii tolfetanusem>, dei Monti della Tolfa e <em>Microtus (Terricola savii niethammericusem>, del Gargano.

  18. Ancylostoma duodenale em estrangeiros radicados em Botucatu, SP, Brasil

    Florence F.S. Kerr

    1981-06-01

    Full Text Available Utilizando-se a técnica de Harada & Mori foi efetuada verificação das espécies de Ancylostomidae prevalentes entre imigrantes europeus, asiáticos e brasileiros residentes no município de Botucatu, Estado de São Paulo, Brasil. Ancylostoma duodenale foi encontrado em 8,5% dos imigrantes asiáticos e Necator americanus em 3,5% dos europeus e em 18% dos brasileiros examinados.

  19. How <em>Varroa> Parasitism Affects the Immunological and Nutritional Status of the Honey Bee, <em>Apis melliferaem>

    Katherine A. Aronstein

    2012-06-01

    Full Text Available We investigated the effect of the parasitic mite <em>Varroa destructorem> on the immunological and nutritional condition of honey bees, <em>Apis melliferaem>, from the perspective of the individual bee and the colony. Pupae, newly-emerged adults and foraging adults were sampled from honey bee colonies at one site in S. Texas, USA. <em>Varroa>‑infested bees displayed elevated titer of Deformed Wing Virus (DWV, suggestive of depressed capacity to limit viral replication. Expression of genes coding three anti-microbial peptides (<em>defensin1, abaecin, hymenoptaecinem> was either not significantly different between <em>Varroa>-infested and uninfested bees or was significantly elevated in <em>Varroa>-infested bees, varying with sampling date and bee developmental age. The effect of <em>Varroa> on nutritional indices of the bees was complex, with protein, triglyceride, glycogen and sugar levels strongly influenced by life-stage of the bee and individual colony. Protein content was depressed and free amino acid content elevated in <em>Varroa>-infested pupae, suggesting that protein synthesis, and consequently growth, may be limited in these insects. No simple relationship between the values of nutritional and immune-related indices was observed, and colony-scale effects were indicated by the reduced weight of pupae in colonies with high <em>Varroa> abundance, irrespective of whether the individual pupa bore <em>Varroa>.

  20. Trabalho em equipa

    Antunes, Andreia Sofia Duarte

    2016-01-01

    O presente relatório de estágio tem como tema principal o trabalho em equipa. Aborda com mais pormenor o subtema dos momentos de reflexão na equipa pedagógica. Ao longo do estudo são vários os conceitos abordados e a interligação entre os mesmos. Pretendo dar a conhecer a temática em questão e conceções que lhe estão naturalmente associadas. Sendo um estudo investigativo são abordados os métodos utilizados ao longo de toda a investigação. Com este relatório pretende-se alcançar qual ...

  1. Toxicidade residual de alguns agrotóxicos recomendado na agricultura sobre Neoseiulus californicus (McGregor (Acari: Phytoseiidae Residual toxicity of some pesticides recommended for citrus orchards on the predaceous mite Neoseiulus californicus (McGregor (Acari: phytoseiidae

    Marcos Zatti da Silva

    2007-04-01

    Full Text Available O objetivo do trabalho foi avaliar a toxicidade residual de alguns agrotóxicos utilizados em citros sobre Neoseiulus californicus (McGregor em condições de laboratório. O método de bioensaio adotado foi o de contato residual. Folhas de citros da variedade Pêra, acondicionadas em arenas, foram pulverizadas em torre de Potter. A toxicidade residual dos produtos foi avaliada duas horas e 1; 3; 5; 7; 10; 14 e 21 dias após a aplicação. Em cada arena, foram transferidas dez fêmeas adultas de N. californicus, juntamente com uma quantidade suficiente de Tetranychus urticae, como fonte de alimento. As avaliações de mortalidade foram realizadas 72 horas após a transferência dos ácaros para as arenas. Os agrotóxicos acrinathrin, deltamethrin, dinocap, enxofre, fenpropathrin, óxido de fenbutatin e propargite não causaram mortalidades significativas em adultos de N. californicus. Foram registradas mortalidades de 29,8; 24,0 e 34,1% para ácaros N. californicus expostos a resíduos de duas horas de idade de abamectim, azocyclotin e cyhexatin, respectivamente. Dicofol, pyridaben e chlorfenapyr causaram 100% de mortalidade aos ácaros predadores expostos aos resíduos tóxicos dos acaricidas, com duas horas de idade. Abamectin provocou mortalidade significativa por um período inferior a um dia. Resíduos dos acaricidas azocyclotin, cyhexatin, dicofol, pyridaben e chlorfenapyr provocaram mortalidades significativas por períodos de 1; 1; 10; 10 e 21dias, respectivamente. Os resultados obtidos no presente experimento servem de subsídio para a escolha adequada dos agrotóxicos a serem utilizados em pomares de citros nos quais N. californicus esteja presente ou naqueles em que o predador venha a ser liberado. Esses resultados também servem para a escolha do momento mais favorável para a liberação dos ácaros predadores dessa espécie no campo, após a aplicação de agrotóxicos nos pomares. Estudos conduzidos em condições de campo ainda s

  2. Comparação da eficiência de diferentes armadilhas utilizadas para a captura de Dermanyssus gallinae (Acari: Dermanyssidae) (de Geer, 1778) Comparison of different sampling traps for Dermanyssus gallinae (Acari: Dermanyssidae) (de Geer, 1778)

    Lucas M. Cunha; Mariana M. Cunha; Romário C. Leite; Silva, Israel J.; Paulo R. Oliveira

    2009-01-01

    O presente trabalho teve como objetivo comparar a eficiência de armadilhas de papel corrugado com a de armadilhas de colmo de bambu taquaril (Phyllostachys sp.) seco na captura de Dermanyssus gallinae, presentes em uma bateria de gaiolas de metal utilizadas na criação de galinhas de postura. A presença de ovos no interior de cada tipo de armadilha foi comparada por meio do teste de Qui-Quadrado e pelo intervalo de confiança para duas proporções. Os valores das somas de estágios móveis nas dif...

  3. Spatial based Expectation Maximizing (EM)

    Balafar M A

    2011-01-01

    Abstract Background Expectation maximizing (EM) is one of the common approaches for image segmentation. Methods an improvement of the EM algorithm is proposed and its effectiveness for MRI brain image segmentation is investigated. In order to improve EM performance, the proposed algorithms incorporates neighbourhood information into the clustering process. At first, average image is obtained as neighbourhood information and then it is incorporated in clustering process. Also, as an option, us...

  4. Synthesis and Spectroscopic Analysis of Novel 1<em>H-Benzo[d>]imidazoles Phenyl Sulfonylpiperazines

    Amjad M. Qandil

    2012-05-01

    Full Text Available A group of benzimidazole analogs of sildenafil, 3-benzimidazolyl-4-methoxy-phenylsulfonylpiperazines 2–4 and 3-benzimidazolyl-4-methoxy-<em>N,N>-dimethyl- benzenesulfonamide (5, were efficiently synthesized. Compounds 2–5 were characterized by NMR and MS and contrary to the reported mass spectra of sildenafil, the spectra of the piperazine-containing compounds 2–4 showed a novel fragmentation pattern leading to an <em>m/z> = 316. A mechanism for the formation of this fragment was proposed.

  5. Oxicenus maxwelli (Keifer (Acari: Eriophyidae danificando a cultura da oliveira, Olea europaea L., no Estado do Rio Grande do Sul Oxicenus maxwelli (Keifer (Acari: Eriophyidae damaging olive tree, Olea europaea L., in Rio Grande do Sul State

    Marcelo Perrone Ricalde

    2012-05-01

    Full Text Available O micro ácaro da oliveira Oxicenus maxwelli (Keifer, 1939 foi identificado em pomares de oliveira nos municípios de Santana do Livramento, Bagé, Candiota, Pelotas e Rio Grande, RS, nos meses de novembro de 2010 a fevereiro de 2011. A espécie foi encontrada nas faces superior e inferior de folhas jovens, em flores e brotos, onde causa deformações, queda de folhas e flores, diminuição da fotossíntese e superbrotação, prejudicando a produção da planta e a comercialização dos frutos deformados. Este é o primeiro registro da espécie atacando a oliveira no Estado do Rio Grande do Sul.The olive bud mite Oxicenus maxwelli (Keifer, 1939 was identified in olive groves in the municipalities of Santana do Livramento, Bagé, Candiota, Pelotas and Rio Grande, RS in November 2010 to February 2011. The specie was found on the upper surface and under surface of young leaves, flowers and buds where it causes deformities, loss of leaves and flowers, decreased photosynthesis and budding, damaging the plant's production and marketing of deformed fruits. This is the first record of the species attacking the olive tree in the state of Rio Grande do Sul.

  6. New host records for Amblyomma rotundatum (Acari: Ixodidae from Grussaí restinga, Rio de Janeiro, Brazil Novos registros de hospedeiros para Amblyomma rotundatum (Acari: Ixodidae da restinga de Grussaí, Rio de Janeiro, Brasil

    Lúcio André Viana

    2012-09-01

    Full Text Available Amblyomma rotundatum Koch is a parthenogenetic tick usually associated with reptiles and amphibians. However, relatively few studies on occurrences of ticks in wild reptile populations in Brazil have been produced. The aim of this study was to analyze the presence of ticks associated with reptile species in the Grussaí restinga, in the municipality of São João da Barra, state of Rio de Janeiro, Brazil. Between December 2010 and January 2011, 131 individuals belonging to nine species of reptiles of the order Squamata were sampled: the lizards Tropidurus torquatus (n = 51, Hemidactylus mabouia (n = 25, Mabuya agilis (n = 30, Mabuya macrorhyncha (n = 6, Cnemidophorus littoralis (n = 5 and Ameiva ameiva (n = 10; and the snakes Philodryas olfersii (n = 2, Oxyrhopus rhombifer (n = 1 and Micrurus corallinus (n = 1. The only tick species found to be associated with any of the reptiles sampled was A. rotundatum. One adult female was detected on one individual of the lizard A. ameiva, one nymph on one individual of the lizard T. torquatus and four nymphs on one individual of the snake P. olfersii. This study is the first record of parasitism of A. rotundatum involving the reptiles T. torquatus and P. olfersii as hosts. Our results suggest that in the Grussaí restinga habitat, A. rotundatum may use different species of reptiles to complete its life cycle.Amblyomma rotundatum Koch é um carrapato partenogenético geralmente associado a répteis e anfíbios. Entretanto existem relativamente poucos estudos sobre a ocorrência de carrapatos em populações silvestres de répteis no Brasil. O objetivo deste estudo foi analisar a presença de carrapatos associados às espécies de répteis em uma comunidade na restinga de Grussaí, município de São João da Barra, Estado do Rio de Janeiro, Brasil. Foram amostradas, entre os meses de dezembro de 2010 e janeiro de 2011, 131 indivíduos pertencentes a nove espécies de répteis da ordem Squamata: lagartos

  7. Proximate Composition, Nutritional Attributes and Mineral Composition of <em>Peperomia> <em>pellucida> L. (Ketumpangan Air Grown in Malaysia

    Maznah Ismail

    2012-09-01

    Full Text Available This study presents the proximate and mineral composition of <em>Peperomia> <em>pellucida> L., an underexploited weed plant in Malaysia. Proximate analysis was performed using standard AOAC methods and mineral contents were determined using atomic absorption spectrometry. The results indicated <em>Peperomia> <em>pellucida> to be rich in crude protein, carbohydrate and total ash contents. The high amount of total ash (31.22% suggests a high-value mineral composition comprising potassium, calcium and iron as the main elements. The present study inferred that <em>Peperomia> <em>pellucida> would serve as a good source of protein and energy as well as micronutrients in the form of a leafy vegetable for human consumption.

  8. Ultrasound-Assisted Extraction of Syringin from the Bark of <em>Ilex em>rotunda> Thumb Using Response Surface Methodology

    Hui Wang; Li-Chun Zhao; Wei Li; Xin Deng; Xiang-Hua Xia; Jian Liang; Geng-Liang Yang; Ying He

    2012-01-01

    In this work, a rapid extraction method based on ultrasound-assisted extraction (UAE) of syringin from the bark of <em>Ilex rotunda em>Thumb using response surface methodology (RSM) is described. The syringin was analyzed and quantified by high performance liquid chromatography coupled with UV detection (HPLC-UV). The extraction solvent, extraction temperature and extraction time, the three main factors for UAE, were optimized with Box-Behnken design (BBD) to obtain the highest ...

  9. Chemical Composition, Antimicrobial and Antitumor Activities of the Essential Oils and Crude Extracts of<em> Euphorbiaem>> macrorrhizaem>

    Haji Akber Aisa

    2012-05-01

    Full Text Available The present study aimed to examine the chemical composition and biological activity of essential oils extracted from <em>Euphorbia macrorrhizaem> collected from Northwest China. The major constituents of the essential oils of aerial parts and roots of <em>E. macrorrhizaem> are acorenone B (16.72% and 25.80%, (+-cycloisosativene (14.94% and 12.40%, 3a-hydroxy-5b-androstane (10.62% and 5.52%, copaene (7.37% and 6.29%, l-calamenene (4.13% and 4.65% and β-cedrene (8.40% and 7.98%, respectively. The minor components of them are thymene, γ-terpinene, thymecamphor, α-cedrene, zingiberene, trans-caryophyllene, β-chamigrene, curcumene, pentadecane, (−-α-muurolene, cuparene, γ-cadinene, (<em>Z>-3-heptadecene, 1,3,7,7-tetramethyl-2-oxabicyclo(4.4.0dec-5-en-4-one, hexahydrofarnesyl acetone, γ-elixene and palmitinic acid. The antimicrobial and antitumor activitiy of the <em>E. macrorrhizaem> essential oil against <em>Staphyloccocus aureusem>, <em>Escherichia coliem>, <em>Canidia Albicansem> and Caco-2 cells were evaluated. Among all the tested microorganisms and Caco-2 cells, the essential oils showed the strongest inhibitory effect on <em>Staphyloccocus aureus em>(MIC = 2.8 μg/mL and Caco-2 cell (IC50 = 11.86 μg/mL, whereas no effect on <em>Escherichia coliem> and <em>Candida em>albicans>. The data of this study suggested that the <em>E. macrorrhizaem> essential oils have great potential as a natural medicine for microbial infections and cancers.

  10. Effects of <em>Citrus aurantiumem> (Bitter Orange Fruit Extracts and <em>p>-Synephrine on Metabolic Fluxes in the Rat Liver

    Rosane Marina Peralta

    2012-05-01

    Full Text Available The fruit extracts of <em>Citrus aurantiumem> (bitter orange are traditionally used as weight-loss products and as appetite supressants. An important fruit component is <em>p>-synephrine, which is structurally similar to the adrenergic agents. Weight-loss and adrenergic actions are always related to metabolic changes and this work was designed to investigate a possible action of the <em>C. aurantiumem> extract on liver metabolism. The isolated perfused rat liver was used to measure catabolic and anabolic pathways, including oxygen uptake and perfusion pressure. The <em>C. aurantiumem> extract and <em>p>-synephrine increased glycogenolysis, glycolysis, oxygen uptake and perfusion pressure. These changes were partly sensitive to a- and b-adrenergic antagonists. <em>p>-Synephrine (200 mM produced an increase in glucose output that was only 15% smaller than the increment caused by the extract containing 196 mM <em>p>-synephrine. At low concentrations the <em>C. aurantiumem> extract tended to increase gluconeogenesis, but at high concentrations it was inhibitory, opposite to what happened with <em>p>-synephrine. The action of the <em>C. aurantiumem> extract on liver metabolism is similar to the well known actions of adrenergic agents and can be partly attributed to its content in <em>p>-synephrine. Many of these actions are catabolic and compatible with the weight-loss effects usually attributed to <em>C. aurantiumem>.

  11. Antioxidant Profile of <em>Trifolium pratenseem> L.

    Heidy Schwartsova

    2012-09-01

    Full Text Available In order to examine the antioxidant properties of five different extracts of <em>Trifolium pratenseem> L. (Leguminosae leaves, various assays which measure free radical scavenging ability were carried out: 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, superoxide anion and nitric oxide radical scavenger capacity tests and lipid peroxidation assay. In all of the tests, only the H2O and (to some extent the EtOAc extracts showed a potent antioxidant effect compared with BHT and BHA, well-known synthetic antioxidants. In addition, <em>in vivo em>experiments were conducted with antioxidant systems (activities of GSHPx, GSHR, Px, CAT, XOD, GSH content and intensity of LPx in liver homogenate and blood of mice after their treatment with extracts of <em>T. pratenseem> leaves, or in combination with CCl4. Besides, in the extracts examined the total phenolic and flavonoid amounts were also determined, together with presence of the selected flavonoids: quercetin, luteolin, apigenin, naringenin and kaempferol, which were studied using a HPLC-DAD technique. HPLC-DAD analysis showed a noticeable content of natural products according to which the examined <em>Trifolium pratenseem> species could well be regarded as a promising new source of bioactive natural compounds, which can be used both as a food supplement and a remedy.

  12. Casca de soja em dietas para coelhos em crescimento

    Ana Carolina Kohlrausch Klinger

    2015-01-01

    Full Text Available O presente trabalho teve como objetivo avaliar efeitos da inclusão de casca de soja em substituição ao feno de alfafa sobre o desempenho, características da carcaça e trato gastrointestinal e parâmetros bioquímicos de coelhos em fase de crescimento. Foram utilizados 30 coelhos da raça Nova Zelândia Branca, divididos igualmente em três tratamentos, compostos pela dieta experimental padrão (sem inclusão de casca de soja; e pelas dietas com 50% e 100% de substituição de alfafa por casca de soja nas respectivas rações experimentais. Concluiu-se que a casca de soja pode ser incluída em dietas para coelhos em crescimento, em substituição ao feno de alfafa em até 100% sem causar danos no desempenho nas características de carcaça e do trato gastrointestinal e parâmetros bioquímicos dos animais, durante a fase de crescimento. Porém, a utilização deste co-produto exige a necessidade de estudos mais aprofundados em virtude da escassez de dados.

  13. Plasticidade alimentar em Rivulus pictus Costa (Osteichthtyes, Cyprinodontiformes, Rivulidae) de uma pequena lagoa em Brasília, Distrito Federal, Brasil

    Shibatta Oscar Akio; Bennemann Sirlei Terezinha

    2003-01-01

    Rivulus pictus has a wide distribution in Distrito Federal and lives in different environments, including small lakes. The analysis of gastric and intestinal contents showed that most of the 21 ingested food items were from autochthonous origin and characterized the diet of an omnivorous fish with a trend to planktivory. Five feeding items were important in the composition of the alimentary spectrum and revealed that food resources are explored near water surface (Acari), in the water column ...

  14. A guerra em Pessoa

    Frias, Joana Matos

    2014-01-01

    Leitura e comentário crítico do poema "Os jogadores de xadrez", do heterónimo pessoano Ricardo Reis, com vista a uma proposta de sistematização da natureza da relação que o poeta manteve com os eventos em curso nos anos decisivos da sua formação e consolidação artísticas e literárias. Reading and critical review of the poem "The chess players", by Fernando Pessoaapos;s heteronym Ricardo Reis, aiming to propose a systematization of the kind of relationship the poet has maintained with the o...

  15. Envolvimento parental em creche

    Oliveira, Marta Isabel da Silva e

    2014-01-01

    Dissertação de mestrado em Educação de Infância A família e a creche são os dois primeiros ambientes sociais que proporcionam à criança estímulos e condutas, sendo, consequentemente, instituições fundamentais no seu crescimento. É importante promover uma boa comunicação entre a creche e os pais, incentivando-os a participarem na educação dos seus filhos, demonstrando o quanto é importante para as crianças este seu envolvimento. Cabe, também, aos educadores/profissionais criar c...

  16. Sustentabilidade em Hotelaria

    Almeida, Joana Branquinho Ramos de

    2016-01-01

    A grande ascensão da atividade turística ao longo das últimas décadas é um dos fenómenos económicos, sociais e culturais mais notáveis de todos os tempos. Durante muitos anos os agentes económicos do sector ignoraram os impactos ambientais desta atividade, contudo, o crescimento expressivo que se tem verificado em conjunto com as alterações climáticas, tornaram evidentes algumas sérias ameaças sociais, ambientais e culturais nos destinos turísticos mais vulneráveis. A sustentab...

  17. Frenectomia em foco

    Vieira, Joana Isabel Monteiro de Paiva

    2012-01-01

    O freio é uma estrutura anatómica oral classificada em freio labial (médio maxilar ou mandibular, lateral maxilar ou mandibular) ou lingual que se pode tornar num problema quando a sua inserção cria impedimentos estruturais e dificuldades a vários níveis. Um freio labial anormal pode causar algumas anomalias ou problemas, tais como um diastema interincisal, problemas protéticos, doença periodontal relacionada com a retenção de alimentos, dificuldades na higiene oral e na mobilidade labial. Na...

  18. Laserterapia em Implantologia

    Reis, Rita Silva

    2013-01-01

    A palavra LASER é um acrónimo de “Light Amplification by Simulated Emission of Radiation”, que significa a amplificação da radiação luminosa por um processo de emissão estimulada. Este dispositivo produz radiação eletromagnética com algumas características especiais, que a diferem de outras fontes de radiação luminosa, sendo monocromática, coerente e colimada. A maioria da literatura relacionada com o LASER distingue-o em dois grandes grupos: os de alta intensidade e os de baixa intensidade. ...

  19. Burnout em Cuidadores Formais

    Silva, Juliana Marisa Fernandes

    2016-01-01

    Dissertação apresentada à Universidade Fernando Pessoa como parte dos requisitos para a obtenção do grau de Mestre em Psicologia, ramo de Psicologia Clínica e da Saúde Este estudo é sobre a Síndrome de Burnout e teve como objetivo geral compreender se o Burnout está presente nos cuidadores formais da Santa Casa da Misericórdia de Castelo de Paiva e quais as variáveis socioprofissionais que poderão exercer influência no seu aparecimento. Pretendeu-se avaliar o Burnout dos cuidadores a tr...

  20. <em>Ipomoea aquaticaem> Extract Shows Protective Action Against Thioacetamide-Induced Hepatotoxicity

    Hadi, A. Hamid A.; Siddig Ibrahim Abdelwahab; Suzy Munir Salama; Salim Said Alkiyumi; Mahmood Ameen Abdullah; Ahmed Salim Alrashdi

    2012-01-01

    In the Indian system of traditional medicine (Ayurveda) it is recommended to consume <em>Ipomoea em>aquatica> to mitigate disorders like jaundice. In this study, the protective effects of ethanol extract of <em>I. aquaticaem> against liver damage were evaluated in thioacetamide (TAA)-induced chronic hepatotoxicity in rats. There was no sign of toxicity in the acute toxicity study, in which Sprague-Dawley (SD) rats were orally fed with <em>...

  1. O insight em psiquiatria

    Ana Margarida P. Cardoso

    2008-12-01

    Full Text Available O sinal de que algo está a acontecer contribui para que o paciente reconheça que alguma coisa de estranho se está a passar consigo. Este reconhecimento faz com que o sujeito possa desempenhar uma função activa e seja um elemento colaborante do seu processo de recuperação. Cada doença apresenta, contudo, diferentes sintomas, uma vez que cada doença psiquiátrica consiste em diferentes perturbações com diversos efeitos sobre o funcionamento mental. Desta maneira, o fenómeno do insight que é registado em cada doença é diferente e expressa-se sob diferentes formas, não somente devido às manifestações clínicas da doença mas também devido às características individuais do sujeito.

  2. Identificazione di due polimorfismi mitocondriali utili alla discriminazione tra <em>Martes martesem> e <em>Martes foinaem>

    Licia Colli

    2003-10-01

    Full Text Available La famiglia Mustelidae è presente sul territorio italiano con 7 specie autoctone (<em>Meles melesem>, <em>Mustela ermineaem>, <em>M. nivalisem>, <em>M. putoriusem>, <em>Lutra lutraem>, <em>Martes foinaem>, <em>M. martesem> ed una naturalizzata (<em>Mustela visonem>. In tassonomia, variazioni morfologiche e morfometriche, spesso a carattere "locale", possono invalidare i tradizionali metodi di diagnosi utilizzati nell'attribuzione specifica. Inoltre criteri di discriminazione legati a caratteristiche corporee macroscopiche non sono applicabili nel caso in cui si disponga unicamente di resti quali peli, depositi fecali, tracce ematiche ecc. Nell'ambito dei mustelidi sono noti fenomeni di sovrapposizione dei caratteri diagnostici che rendono problematica l'identificazione delle specie, come nel caso di <em>M. martesem> e <em>M. foinaem>. Negli ultimi anni l'identificazione di polimorfismi genetico-molecolari a scopo tassonomico ha permesso di dirimere numerosi casi incerti. In questo lavoro sono presentati i dati preliminari di uno studio volto ad identificare polimorfismi discriminanti le due specie di mustelidi considerate. L'analisi è stata condotta con enzimi di restrizione applicati ad una regione del genoma mitocondriale. Parte della sequenza del gene citocromo b (cyt b è stata amplificata con PCR e <em>primers> universali, ottenendo un frammento di circa 360 bp. Gli enzimi di restrizione impiegati nell'analisi sono stati scelti in accordo con profili di digestione teorici, ricavati con un apposito software a partire da sequenze di cyt b di <em>M. martesem> e <em>M. foinaem> presenti in banche dati. A seguito della digestione sequenziale con gli enzimi Hae III e Bgl I, il profilo elettroforetico generato mostra la presenza di una banda di circa 180 bp negli individui appartenenti alla specie <em>M. martesem> e di due bande distinte di circa 130 e 230 bp negli esemplari di <em>M. foinaem>. Il campione da noi

  3. Biology of three species of Agistemus (Acari: Stigmaeidae): life table parameters using eggs of Panonychus citri or pollen of Malephora crocea as food.

    Goldarazena, Arturo; Aguilar, Hugo; Kutuk, Halil; Childers, Carl C

    2004-01-01

    The biology and life table parameters of Agistemus industani Gonzalez, A. cyprius Gonzalez, and A. floridanus Gonzalez (Acari: Stigmaeidae) were studied under laboratory conditions using two food sources: Panonychus citri (McGregor) eggs or ice plant, Malephora crocea (Jacquin) Schwantes pollen at 25 degrees C. The larval, protonymph, deutonymph, and adult stages of A. industani fed on citrus red mite eggs. All active stages of A. industani, except the larva, fed on all P. citri stages and the larval stage could not feed on P. citri adults. All immature stages of A. industani fed on M. crocea pollen. Agistemus cyprius larvae fed on P. citri eggs and larvae or ice plant pollen. The nymphal stages fed on P. citri eggs, larvae, and protonymphs but not deutonymphs or adults while A. cyprius deutonymphs and adults fed on all P. citri stages. Adult and nymphal stages of A. cyprius fed on ice plant pollen and successfully completed their development while A. floridanus did not. Agistemus floridanus larvae fed only on P. citri eggs, while the other stages fed on P. citri eggs, larvae, and protonymphs. The developmental times from egg to adult for A. industani and A. cyprius when fed M. crocea pollen were 11.3 and 13.4 days, respectively. Agistemus floridanus was unable to complete its life cycle on a diet of only M. crocea pollen. Agistemus industani, A. cyprius, and A. floridanus completed development from egg to adult in 11.7, 13.8, and 10.8 days, respectively, when fed P. citri eggs. The intrinsic rate of increase (r(m)) values for A. cyprius and A. industani were 0.0311 and 0.1201 per day on the pollen diet. The net reproductive rate (Ro) was 3.58 for A. cyprius and 10.07 for A. industani with generation times (T) of 45.2 and 35.1 days, respectively, on the ice plant pollen diet. The r(m) values for A. cyprius, A. floridanus, and A. industani on the P. citri egg only diet were: 0.0562, 0.1001, and 0.1031 per day, respectively. The Ro values for each species fed P

  4. Potential of two populations of Amblyseius swirskii (Acari: Phytoseiidae) for the control of Bemisia tabaci biotype B (Hemiptera: Aleyrodidae) in Brazil.

    Cavalcante, Ana Cristina Cerqueira; Borges, Lucas Rosa; Lourenção, André Luiz; de Moraes, Gilberto José

    2015-12-01

    Predatory mites of the family Phytoseiidae are commercialized in European and North American countries for the control of whiteflies (Insecta: Aleyrodidae). Amblyseius swirskii Athias-Henriot (Acari: Phytoseiidae) is one of the predators used for that purpose. This predator is not found in Brazil and in many other countries, but its introduction could promote biological control of Bemisia tabaci (Gennadius) biotype B (silverleaf whitefly) in those countries. The aim of this study was to compare two populations of A. swirskii as predators of eggs of B. tabaci of two different ages, as well as the acceptance of those populations for other food types [eggs of Tetranychus urticae Koch; larvae and protonymphs of Aleuroglyphus ovatus (Troupeau) (Astigmatina: Acaridae) and cattail pollen (Typha domingensis Persoon)]. One of the populations of A. swirskii was collected in the Republic of Benin, in tropical Africa, and the other was commercially available in The Netherlands. The comparison was done to evaluate the population with better potential for practical use, and to determine food types that could be used for mass rearing the predator. Experiments were conducted at 28 ± 1 °C, 80 ± 10 % relative humidity and 12 h photophase. Both populations were able to oviposit when fed eggs of B. tabaci, but the Netherlands population showed higher oviposition rate (2.3 and 1.6 times higher on young and older eggs, respectively). Higher predation rates were observed for eggs up to 24 h of age in comparison with older eggs. Aleuroglyphus ovatus proved to be a promising prey for possible mass rearing of this predator in Brazil, where that astigmatic mite is found naturally when pollen was used as food, no significant difference was observed for oviposition rate between populations. The results suggested a higher potential of the Netherlands population of A. swirskii for the control of B. tabaci biotype B in this country for augmentative biological control, with the possible use of

  5. Phytoseiidae (Acari: Mesostigmata) within citrus orchards in Florida: species distribution, relative and seasonal abundance within trees, associated vines and ground cover plants.

    Childers, Carl C; Denmark, Harold A

    2011-08-01

    Seven citrus orchards on reduced- to no-pesticide spray programs were sampled for predacious mites in the family Phytoseiidae (Acari: Mesostigmata) in central and south central Florida. Inner and outer canopy leaves, open flowers, fruit, twigs, and trunk scrapings were sampled monthly between September 1994 and January 1996. Vines and ground cover plants were sampled monthly between September 1994 and January 1996 in five of these orchards. The two remaining orchards were on full herbicide programs and ground cover plants were absent. Thirty-three species of phytoseiid mites were identified from 35,405 specimens collected within citrus tree canopies within the seven citrus orchards, and 8,779 specimens from vines and ground cover plants within five of the seven orchards. The six most abundant phytoseiid species found within citrus tree canopies were: Euseius mesembrinus (Dean) (20,948), Typhlodromalus peregrinus (Muma) (8,628), Iphiseiodes quadripilis (Banks) (2,632), Typhlodromips dentilis (De Leon) (592), Typhlodromina subtropica Muma and Denmark (519), and Galendromus helveolus (Chant) (315). The six most abundant species found on vines or ground cover plants were: T. peregrinus (6,608), E. mesembrinus (788), T. dentilis (451), I. quadripilis (203), T. subtropica (90), and Proprioseiopsis asetus (Chant) (48). The remaining phytoseiids included: Amblyseius aerialis (Muma), A. herbicolus (Chant), A. largoensis (Chant), A. multidentatus (Chant), A. sp. near multidentatus, A. obtusus (Koch), Chelaseius vicinus (Muma), Euseius hibisci Chant, Galendromus gratus (Chant), Metaseiulus mcgregori (Chant), Neoseiulus mumai (Denmark), N. vagus (Denmark), Phytoscutus sexpilis (Muma), Phytoseiulus macropilis (Banks), Proprioseiopsis detritus (Muma), P. dorsatus (Muma), P. macrosetae (Banks), P. rotundus (Muma), P. solens (De Leon), Typhlodromips deleoni (Muma), T. dillus (De Leon), T. dimidiatus (De Leon), T. mastus Denmark and Muma, T. simplicissimus (De Leon), and T. sp

  6. Eupalopsellidae and Stigmaeidae (Acari: Prostigmata) within citrus orchards in Florida: species distribution, relative and seasonal abundance within trees, associated vines, and ground cover plants.

    Childers, Carl C; Ueckermann, Eduard A

    2014-10-01

    Seven citrus orchards on reduced- to no-pesticide spray programs were sampled for predacious mites in the families Eupalopsellidae and Stigmaeidae (Acari: Prostigmata) in central and south central Florida. Inner and outer canopy leaves, fruit, twigs, and trunk scrapings were sampled monthly between August 1994 and January 1996. Open flowers were sampled in March from five of the sites. Two species of eupalopsellid mites (Exothorhis caudata Summers and Saniosulus harteni (van-Dis and Ueckermann)) were identified from 252 specimens collected within citrus tree canopies within the seven citrus orchards of which 249 were E. caudata. Only two E. caudata were collected from ground cover plants within five of the seven orchards. Eight species of Stigmaeidae were identified from 5,637 specimens: Agistemus floridanus Gonzalez, A. terminalis Gonzalez, Eustigmaeus arcuata (Chandhri), E. sp. near arcuata, E. segnis (Koch), Mediostigmaeus citri (Rakha and McCoy), Stigmaeus seminudus Wood, and Zetzellia languida Gonzalez were collected from within citrus tree canopies from seven orchard sites. Agistemus floridanus was the only species in either family that was abundant with 5,483 collected from within citrus tree canopies compared with only 39 from vine or ground cover plants. A total of 431 samples from one or more of 82 vines and ground cover plants were sampled monthly between September 1994 and January 1996 in five of these orchards and one or more eupalopsellids or stigmaeids were collected from 19 of these plants. Richardia brasiliensis (Meg.) Gomez had nine A. floridanus from 5 of 25 samples collected from this plant. Solanum sp. had five A. floridanus from three samples taken. Both eupalopsellid and stigmaeid species numbers represented orchards were on full herbicide programs and ground cover plants were absent. Agistemus floridanus was more abundant in the citrus orchards with on-going or recent herbicide programs compared with orchards having well-developed ground

  7. Comparative Proteomic Analysis of <em>Lactobacillus> <em>plantarum> WCFS1 and Δ<em>ctsR> Mutant Strains Under Physiological and Heat Stress Conditions

    Daniela Fiocco

    2012-08-01

    Full Text Available Among Gram-positive bacteria, CtsR (Class Three Stress gene Repressor mainly regulates the expression of genes encoding the Clp ATPases and the ClpP protease. To gain a better understanding of the biological significance of the CtsR regulon in response to heat-shock conditions, we performed a global proteomic analysis of <em>Lactobacillus> <em>plantarum> WCFS1 and ∆<em>ctsR> mutant strains under optimal or heat stress temperatures. Total protein extracts from bacterial cells were analyzed by two-dimensional gel fractionation. By comparing maps from different culture conditions and different <em>L.> <em>plantarum> strains, image analysis revealed 23 spots with altered levels of expression. The proteomic analysis of <em>L.> <em>plantarum> WCFS1 and <em>ctsR> mutant strains confirms at the translational level the CtsR-mediated regulation of some members of the Clp family, as well as the heat induction of typical stress response genes. Heat activation of the putative CtsR regulon genes at transcriptional and translational levels, in the <em>∆ctsR> mutant, suggests additional regulative mechanisms, as is the case of <em>hsp1em>. Furthermore, isoforms of ClpE with different molecular mass were found, which might contribute to CtsR quality control. Our results could add new outlooks in order to determine the complex biological role of CtsR-mediated stress response in lactic acid bacteria.

  8. Avaliação in vitro de uma cepa de campo de Boophilus microplus (Acari : Ixodidae resistente à amitraz

    Santamaría Vargas Minerva

    2003-01-01

    Full Text Available O uso dos acaricidas químicos ainda se constitui no principal instrumento de controle do carrapato bovino Boophilus microplus. No sul do Brasil, o amitraz aplicado por imersão e aspersão é o ingrediente ativo mais utilizado, nos últimos anos, contra as cepas de carrapatos resistentes aos organofosforados (OF e piretróides sintéticos (PS. Em conseqüência, torna-se importante a realização de investigações que possam contribuir para prolongar a vida útil desse ixodicida. No presente estudo, foi analisado o comportamento toxicológico de uma cepa de B. microplus colhida na localidade de Alegrete, Rio Grande do Sul, usando-se químicos da família dos OF, PS, misturas OF/PS, além do amitraz. Os resultados indicaram que a cepa denominada "Santa Luiza" apresentou fatores de resistência (FR que variaram entre 2,3 e 3,95 para OF, 23,3 e 147,56 para PS e de 3,76 a 21.57 para amitraz em testes realizados com larvas de carrapatos. A caracterização e purificação de uma cepa de B. microplus resistente ao amitraz permitem seu uso como cepa de referência para a avaliação biológica de ixodicidas alternativos, além de padronizar e validar métodos de diagnósticos toxicológicos que possam detectar resistência frente a esse acaricida.

  9. Ancylostoma duodenale em estrangeiros radicados em Botucatu, SP, Brasil

    Florence F.S. Kerr; Corrêa, Fernando M. A.

    1981-01-01

    Utilizando-se a técnica de Harada & Mori foi efetuada verificação das espécies de Ancylostomidae prevalentes entre imigrantes europeus, asiáticos e brasileiros residentes no município de Botucatu, Estado de São Paulo, Brasil. Ancylostoma duodenale foi encontrado em 8,5% dos imigrantes asiáticos e Necator americanus em 3,5% dos europeus e em 18% dos brasileiros examinados.A survey of the species of Ancylostomidae causing infection among European inmigrants and Brazilian residents in Botucatu, ...

  10. Short and Long-Term Effects of <em>Baccharis articulataem> on Glucose Homeostasis

    Flávio H. Reginatto

    2012-06-01

    Full Text Available In this study, the<em> in vivoem> effect of the crude extract and <em>n>-butanol and aqueous residual fractions of <em>Baccharis articulataem> (Lam. Pers. on serum glucose levels, insulin secretion and liver and muscle glycogen content, as well as<em> in vitroem> action on serum intestinal disaccharidase activity and albumin glycation were investigated. Oral administration of the extract and fractions reduced glycemia in hyperglycemic rats. Additionally, the <em>n>-butanol fraction, which has high flavonoids content, stimulated insulin secretion, exhibiting an insulinogenic index similar to that of glipizide. Also, the <em>n>-butanol fraction treatment significantly increased glycogen content in both liver and muscle tissue. <em>In vitroem> incubation with the crude extract and <em>n>-butanol and aqueous residual fractions inhibited maltase activity and the formation of advanced glycation end-products (AGEs. Thus, the results demonstrated that <em>B. articulataem> exhibits a significant antihyperglycemic and insulin-secretagogue role. These effects on the regulation of glucose homeostasis observed for <em>B. articulataem> indicate potential anti-diabetic properties.

  11. Osteoporose em caprinos

    Fábio B. Rosa

    2013-04-01

    Full Text Available Foi realizado um estudo de casos de osteoporose em caprinos provenientes de uma propriedade na área de abrangência do LPV-UFSM, determinando a epidemiologia, o quadro clínico-patológico e discutindo os prováveis mecanismos patogenéticos. Cinco cabras, fêmeas, SRD, de seis meses a seis anos de idade foram afetadas. As cabras eram mantidas em campo nativo, sem suplementação com ração e sob superlotação. Os principais sinais clínicos foram emagrecimento, dificuldade de locomoção e permanência em decúbito por longos períodos. As principais alterações macroscópicas nos ossos examinados foram vistas nas superfícies de corte e caracterizavam-se por depleção do osso esponjoso (porosidade e redução acentuada da espessura do osso cortical. Havia também marcada atrofia serosa da gordura da medula óssea. Microscopicamente, nas regiões avaliadas (úmero proximal, rádio distal, fêmur distal, tíbia proximal e corpos das vértebras lombares foi observada redução moderada a acentuada do número e da espessura das trabéculas ósseas nas epífises e metáfises dos ossos longos e nos corpos vertebrais. Os achados clínico-patológicos indicaram que a osteoporose observada provavelmente foi causada pela desnutrição. As alterações ósseas (diminuição no número e na espessura das trabéculas do osso esponjoso sugerem que ambos os mecanismos, má formação óssea e reabsorção óssea aumentada, contribuíram para a ocorrência de osteoporose nos caprinos deste estudo.

  12. Synthesis of 1,2,3-Triazole Derivatives and <em>in Vitroem> Antifungal Evaluation on <em>Candida> Strains

    Almir G. Wanderley

    2012-05-01

    Full Text Available 1,2,3-Triazoles have been extensively studied as compounds possessing important biological activities. In this work, we describe the synthesis of ten 2-(1-aryl-1<em>H>-1,2,3-triazol-4-ylpropan-2-ols via copper catalyzed azide alkyne cycloaddition (CuAAc or <em>click chemistryem>. Next the<em> in vitroem> antifungal activity of these ten compounds was evaluated using the microdilution broth method against 42 isolates of four different <em>Candida> species. Among all tested compounds, the halogen substituted triazole 2-[1-(4-chlorophenyl-1<em>H>-(1,2,3triazol-4-yl]propan-2-ol, revealed the best antifungal profile, showing that further modifications could be done in the structure to obtain a better drug candidate in the future.

  13. Em favor da talassografia

    Jean-Louis Boudou

    2001-01-01

    Full Text Available A Talassografia (“descrição do mar” interessa-sepelos impactos físicos, biológicos, ecológicos... culturais da violenta antropização dos ambientes costeiros (oceânicos e continentais, caracterizados pelaexigüidade, vulnerabilidade, fragilidade e plasticidade. Como o Brasil é um “país marítimo”, os geó-grafos (os talassógrafos brasileiros são convidadosa intensificar suas pesquisas nas áreas costeiras e acriar novas estruturas para divulgá-las: Revista, Encontros, Associação, Pós-Graduação... tudo em prolda talassografia.

  14. Coincidental intraguild predation by caterpillars on spider mites.

    Shirotsuka, Kanako; Yano, Shuichi

    2012-01-29

    Intraguild predation (IGP) is defined as the killing and eating of prey species by a predator that also can utilize the resources of the prey. It is mainly reported among carnivores that share common herbivorous prey. However, a large chewing herbivore could prey upon sedentary and/or micro herbivores in addition to utilizing a host plant. To investigate such coincidental IGP, we observed the behavioral responses of the polyphagous mite Tetranychus kanzawai Kishida (Acari: Tetranychidae) when its host plant Cayratia japonica (Thunb.) Gagnep. (Vitaceae) was attacked by hornworms, Theretra japonica Boisduval (Sphingidae) and T. oldenlandiae Fabricius (Sphingidae). We also examined an interaction between the oligophagous mite Panonychus citri McGregor (Acari: Tetranychidae) and caterpillars of the swallowtail Papilio xuthus L. (Papilionidae) that share citrus plants as their main food source. Although all T. kanzawai and some active stage P. citri tried to escape from the coincidental IGP, some were consumed together with eggs, quiescent mites, and host plant leaves, suggesting that coincidental IGP occurs on spider mites in the wild. Moreover, neither hornworms nor swallowtail caterpillars distinguished between spider mite-infested and uninfested leaves, suggesting that the mite-infested leaves do not discourage caterpillar feeding. The reasons that the mites have no effective defense against coincidental IGP other than escaping are discussed. PMID:22286142

  15. Antioxidant Profile of <em>Trifolium pratenseem> L.

    Heidy Schwartsova; Mirjana Vojinovic-Miloradov; Sanja Vlaisavljevic; Mira Popovic; Biljana Kaurinovic

    2012-01-01

    In order to examine the antioxidant properties of five different extracts of <em>Trifolium pratenseem> L. (Leguminosae) leaves, various assays which measure free radical scavenging ability were carried out: 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, superoxide anion and nitric oxide radical scavenger capacity tests and lipid peroxidation assay. In all of the tests, only the H2O and (to some extent) the EtOAc extracts showed a potent antioxidant effect compar...

  16. A Great Barrier Reef <em>Sinularia> sp. Yields Two New Cytotoxic Diterpenes

    Cherie A. Motti

    2012-07-01

    Full Text Available The methanol extract of a <em>Sinularia em>sp., collected from Bowden Reef, Queensland, Australia, yielded ten natural products. These included the new nitrogenous diterpene (4<em>R>*,5<em>R>*,9<em>S>*,10<em>R>*,11<em>Z>-4-methoxy-9-((dimethylamino-methyl-12,15-epoxy-11(13-en-decahydronaphthalen-16-ol (1, and the new lobane, (1<em>R*>,2<em>R*>,4<em>S*>,15<em>E>-loba-8,10,13(14,15(16-tetraen-17,18-diol-17-acetate (2. Also isolated were two known cembranes, sarcophytol-B and (1<em>E>,3<em>E>,7<em>E>-11,12-epoxycembratrien-15-ol, and six known lobanes, loba-8,10,13(15-triene-16,17,18-triol, 14,18-epoxyloba-8,10,13(15-trien-17-ol, lobatrientriol, lobatrienolide, 14,17-epoxyloba-8,10,13(15-trien-18-ol-18-acetate and (17<em>R>-loba-8,10,13(15-trien-17,18-diol. Structures of the new compounds were elucidated through interpretation of spectra obtained after extensive NMR and MS investigations and comparison with literature values. The tumour cell growth inhibition potential of 1 and 2 along with loba-8,10,13(15-triene-16,17,18-triol, 14,17-epoxyloba-8,10,13(15-trien-18-ol-18-acetate, lobatrienolide, (1<em>E>,3<em>E>,7<em>E>-11,12-epoxycembratrien-15-ol and sarcophytol-B were assessed against three human tumour cell lines (SF-268, MCF-7 and H460. The lobanes and cembranes tested demonstrated 50% growth inhibition in the range 6.8–18.5 µM, with no selectivity, whilst 1 was less active (GI50 70–175 µM.

  17. Regional Suppression of <em>Bactrocera> Fruit Flies (Diptera: Tephritidae in the Pacific through Biological Control and Prospects for Future Introductions into Other Areas of the World

    Roger I. Vargas

    2012-08-01

    Full Text Available <em>Bactrocera> fruit fly species are economically important throughout the Pacific. The USDA, ARS U.S. Pacific Basin Agricultural Research Center has been a world leader in promoting biological control of <em>Bactrocera> spp. that includes classical, augmentative, conservation and IPM approaches. In Hawaii, establishment of <em>Bactrocera> <em>cucurbitae> (Coquillett in 1895 resulted in the introduction of the most successful parasitoid, <em>Psyttalia> <em>fletcheri> (Silvestri; similarly, establishment of <em>Bactrocera> <em>dorsalis> (Hendel in 1945 resulted in the introduction of 32 natural enemies of which <em>Fopius> <em>arisanus> (Sonan, <em>Diachasmimorpha> <em>longicaudata> (Ashmead and <em>Fopius> <em>vandenboschi> (Fullaway were most successful. Hawaii has also been a source of parasitoids for fruit fly control throughout the Pacific region including Australia, Pacific Island Nations, Central and South America, not only for <em>Bactrocera> spp. but also for <em>Ceratitis> and <em>Anastrepha> spp. Most recently, in 2002, <em>F.> <em>arisanus> was introduced into French Polynesia where <em>B.> <em>dorsalis> had invaded in 1996. Establishment of <em>D.> <em>longicaudata> into the new world has been important to augmentative biological control releases against <em>Anastrepha> spp. With the rapid expansion of airline travel and global trade there has been an alarming spread of <em>Bactrocera> spp. into new areas of the world (<em>i.e.>, South America and Africa. Results of studies in Hawaii and French Polynesia, support parasitoid introductions into South America and Africa, where <em>B.> <em>carambolae> and <em>B.> <em>invadens>, respectively, have become established. In addition, <em>P.> <em>fletcheri> is a candidate for biological control of <em>B.> <em>cucurbitae> in Africa. We review past and more

  18. Extraction of Dihydroquercetin<em> em>from <em>Larix gmeliniem>i> em>with Ultrasound-Assisted and Microwave-Assisted Alternant Digestion

    Yuangang Zu

    2012-07-01

    Full Text Available An ultrasound and microwave assisted alternant extraction method (UMAE was applied for extracting dihydroquercetin (DHQ from <em>Larix gmeliniem>i> wood. This investigation was conducted using 60% ethanol as solvent, 1:12 solid to liquid ratio, and 3 h soaking time. The optimum treatment time was ultrasound 40 min, microwave 20 min, respectively, and the extraction was performed once. Under the optimized conditions, satisfactory extraction yield of the target analyte was obtained. Relative to ultrasound-assisted or microwave-assisted method, the proposed approach provides higher extraction yield. The effect of DHQ of different concentrations and synthetic antioxidants on oxidative stability in soy bean oil stored for 20 days at different temperatures (25 °C and 60 °C was compared. DHQ was more effective in restraining soy bean oil oxidation, and a dose-response relationship was observed. The antioxidant activity of DHQ was a little stronger than that of BHA and BHT. Soy bean oil supplemented with 0.08 mg/g DHQ exhibited favorable antioxidant effects and is preferable for effectively avoiding oxidation. The <em>L. gmeliniiem> wood samples before and after extraction were characterized by scanning electron microscopy. The results showed that the UMAE method is a simple and efficient technique for sample preparation.

  19. Molluscicidal Activity of Methomyl and Cardenolide Extracts from <em>Calotropis proceraem> and <em>Adenium> <em>arabicum> Against the Land Snail <em>Monacha cantianaem>

    Alaa Bayoumi

    2012-05-01

    Full Text Available In this work, we have evaluated the molluscicidal activity of two cardenolide extracts from <em>Adenium arabicumem> Balf f. [the benzene (B and methanol (M extracts], one cardenolide extract from <em>Calotropis proceraem> (Aiton W.T. Aiton (extract C, and methomyl against the harmful land snail <em>Monacha cantianaem> (Montagu. The contact LD50 values for the above mentioned plant extracts were 12.62, 34.63, and 34.35 mg·kg−1 of body weight, respectively, while the LD50 for methomyl was 116.62 mg·kg−1, that is, the plant extracts were 9.24, 3.37, and 3.4 times more toxic than methomyl. In addition, a simple colorimetric method, based on Kedde reagent, was modified to determine cardenolide concentrations in plant extracts. Thin layer chromatography analysis (TLC showed several cardiac glycosidal compounds in each plant extract. The results proved that cardiac glycosides are promising candidate compounds that could be used to control land snails, or exploited to develop new, effective, and environmentally friendly molluscicides.

  20. Antioxidative Properties of Crude Polysaccharides from <em>Inonotus obliquusem>

    Jinyou Duan; Guoting Cui; Wuxia Zhang; Haibo Mu; Amin Zhang; Shunchun Wang

    2012-01-01

    The mushroom <em>Inonotus obliquusem>> em>has been widely used as a folk medicine in Russia, Poland and most of the Baltic countries. In this study, water-soluble and alkali-soluble crude polysaccharides (IOW and IOA) were isolated from <em>I. obliquusem>, and the carbohydrate-rich fractions IOW-1 and IOA-1 were obtained respectively after deproteination and depigmentation. Their contents, such as neutral carbohydrate, uronic acid and protein, were...

  1. Morphological description of Amblyomma brasiliense Aragão, 1908 (Acari: Ixodidae larvae and nymphs Descrição morfológica de larvas e ninfas de Amblyomma brasiliense Aragão, 1908 (Acari: Ixodidae

    Gustavo Seron Sanches

    2009-09-01

    Full Text Available The immature stages of Neotropical ticks are poorly known and in many cases have not been described. This work presents a morphological description of Amblyomma brasiliense larvae (F1 and a redescription of nymphs (F1. A. brasiliense is reported as one of the most aggressive ticks to humans in Brazil. Immature ticks obtained from a laboratory colony initiated from adult specimens collected in the Parque Estadual Intervales (24º 18' S and 48º 24' W, São Paulo, Brazil, were analyzed under scanning electron microscope, and also under light and stereoscopic microscopes. A. brasiliense larvae present basis capituli rectangular; short palpi; rounded idiosoma; coxa I with two spurs, the external one being longer than the internal one; and coxae II and III each with one short spur. Nymphs present basis capituli rectangular with a sharp pointed cornua; oval idiosoma with scutum reaching coxa III; coxa I with two evident spurs, the external one being longer than the internal one; coxae II-III each with one short spur; and coxa IV with a very short spur and chitinous tubercles on internal surface of posterior border of idiosoma. These morphological features, in association with chaetotaxy and porotaxy, should make possible the identification of immature Amblyomma ticks.Os estágios imaturos dos carrapatos Neotropicais são pouco conhecidos e, em muitos casos, não estão descritos. Este trabalho apresenta uma descrição morfológica das larvas (F1 e redescrição das ninfas (F1 do carrapato Amblyomma brasiliense, relatado como um dos ixodídeos mais agressivos ao homem no Brasil. Para este fim, carrapatos imaturos de uma colônia iniciada com espécimes adultos do Parque Estadual de Intervales, São Paulo, Brasil foram analisados sob microscopia eletrônica de varredura, microscopia de luz e lupa estereoscópica. Observou-se que larvas têm a base do capítulo retangular, palpos curtos e idiossoma arredondado, coxa I com dois espinhos, sendo o externo

  2. Comparação da eficiência de diferentes armadilhas utilizadas para a captura de Dermanyssus gallinae (Acari: Dermanyssidae (de Geer, 1778 Comparison of different sampling traps for Dermanyssus gallinae (Acari: Dermanyssidae (de Geer, 1778

    Lucas M. Cunha

    2009-12-01

    Full Text Available O presente trabalho teve como objetivo comparar a eficiência de armadilhas de papel corrugado com a de armadilhas de colmo de bambu taquaril (Phyllostachys sp. seco na captura de Dermanyssus gallinae, presentes em uma bateria de gaiolas de metal utilizadas na criação de galinhas de postura. A presença de ovos no interior de cada tipo de armadilha foi comparada por meio do teste de Qui-Quadrado e pelo intervalo de confiança para duas proporções. Os valores das somas de estágios móveis nas diferentes armadilhas a cada intervalo de tempo foram comparados utilizando-se o teste de Wilcoxon. A proporção de armadilhas contendo ovos não foi diferente nos dois tipos de armadilha (p This work aims to compare the performance of corrugated paper and "taquaril" bamboo (Phyllostachys sp. straw traps for collecting (in sampling Dermanyssus gallinae in a metal cages battery laying hens. The presence of eggs in the two trap models were compared using a Qui-square test and a proportion confidence interval test. Total daily values of mobile instars gathered in each type of trap were compared using the Wilcoxon's test. The amount of traps containing eggs was not different in neither of the traps (p < 0,05. The number of mobile instars sampled at every two days per trap model was different (p < 0,01. Counting in the corrugated paper traps showed a Gauss distribution histogram and was superior to the values of the bamboo straw traps.

  3. Antioxidant Activity of Various Parts of <em>Cinnamomum> cassiaem> Extracted with Different Extraction Methods

    Li-Yeh Chuang

    2012-06-01

    Full Text Available The aim of this study was to investigate the antioxidant activities of various parts (barks, buds, and leaves of <em>Cinnamomum cassia em>extracted with ethanol and supercritical fluid extraction (SFE. For the antioxidant activity comparison, IC50 values of the SFE and ethanol extracts in the DPPH scavenging assay were 0.562–10.090 mg/mL and 0.072–0.208 mg/mL, and the Trolox equivalent antioxidant capacity (TEAC values were 6.789–58.335 mmole Trolox/g and 133.039–335.779 mmole Trolox/g, respectively. In addition, the total flavonoid contents were 0.031–1.916 g/ 100 g dry weight of materials (DW and 2.030–3.348 g/ 100 g DW, and the total phenolic contents were 0.151–2.018 g/ 100 g DW and 6.313–9.534 g/ 100 g DW in the SFE and ethanol extracts, respectively. Based on the results, the ethanol extracts of <em>Cinnamon> barks have potential value as an antioxidant substitute and this study also provide a better technique to extract the natural antioxidant substances from <em>C. cassiaem>.

  4. Activity-Guided Isolation of Antioxidant Compounds from <em>Rhizophora apiculataem>

    Hongbin Xiao

    2012-09-01

    Full Text Available <em>Rhizophora apiculataem> (<em>R. apiculataem> contains an abundance of biologically active compounds due its special salt-tolerant living surroundings. In this study, the total phenolic content and antioxidant activities of various extract and fractions of stem of <em>R. apiculataem> were investigated. Results indicated that butanol fraction possesses the highest total phenolic content (181.84 mg/g GAE/g dry extract with strongest antioxidant abilities. Following <em>in vitroem> antioxidant activity-guided phytochemical separation procedures, lyoniresinol-3α-<em>O>-β-arabinopyranoside (1, lyoniresinol-3α-<em>O>-β-rhamnoside (2, and afzelechin-3-<em>O>-L-rhamno-pyranoside (3 were separated from the butanol fraction. These compounds showed more noticeable antioxidant activity than a BHT standard in the DPPH, ABTS and hydroxyl radical scavenging assays. HPLC analysis results showed that among different plant parts, the highest content of 13 was located in the bark (0.068%, 0.066% and 0.011%, respectively. The results imply that the <em>R. apiculataem> might be a potential source of natural antioxidants and 13 are antioxidant ingredients in <em>R. apiculataem>.

  5. Effects of Cyanobacterial Lipopolysaccharides from <em>Microcystis em>on Glutathione-Based Detoxification Pathways in the Zebrafish (<em>Danio rerioem> Embryo

    John P. Berry

    2012-05-01

    Full Text Available Cyanobacteria (“blue-green algae” are recognized producers of a diverse array of toxic secondary metabolites. Of these, the lipopolysaccharides (LPS, produced by all cyanobacteria, remain to be well investigated. In the current study, we specifically employed the zebrafish (<em>Danio rerioem> embryo to investigate the effects of LPS from geographically diverse strains of the widespread cyanobacterial genus, <em>Microcystis>, on several detoxifying enzymes/pathways, including glutathione-S-transferase (GST, glutathione peroxidase (GPx/glutathione reductase (GR, superoxide dismutase (SOD, and catalase (CAT, and compared observed effects to those of heterotrophic bacterial (<em>i.e.>, <em>E. coliem> LPS. In agreement with previous studies, cyanobacterial<em> em>LPS significantly reduced GST in embryos exposed to LPS in all treatments. In contrast, GPx moderately increased in embryos exposed to LPS, with no effect on reciprocal GR activity. Interestingly, total glutathione levels were elevated in embryos exposed to <em>Microcystis> LPS, but the relative levels of reduced and oxidized glutathione (<em>i.e.>, GSH/GSSG were, likewise, elevated suggesting that oxidative stress is not involved in the observed effects as typical of heterotrophic bacterial LPS in mammalian systems. In further support of this, no effect was observed with respect to CAT or SOD activity. These findings demonstrate that <em>Microcystis> LPS affects glutathione-based detoxification pathways in the zebrafish embryo, and more generally, that this model is well suited for investigating the apparent toxicophore of cyanobacterial LPS, including possible differences in structure-activity relationships between heterotrophic and cyanobacterial LPS, and teleost fish <em>versus> mammalian systems.

  6. On the taxonomy and distribution of <em>Crocidura cossyrensisem> and <em>Crocidura russulaem> (Insectivora, Soricidae in Maghreb

    Longino Contoli

    2001-09-01

    Full Text Available Abstract The paper examines the taxonomic status of North African <em>Crocidura russula Auctorumem>. On the basis of morphological data, the taxon needs to be divided into at least two species: <em>C. russulaem> (Hermann, 1780 from western Europe, coastal Morocco and middle-western Algeria, up to about the Algiers area, and possibly even Sardinia and Ibiza, and another species, distributed in coastal middle-eastern Algeria, Tunisia, Pantelleria and, possibly, La Galite archipelago. The first and only available name for the above, eastern taxon is <em>Crocidura cossyrensisem> Contoli, 1989.

  7. Prima segnalazione di <em>Brachylaima fulvusem> Dujardin, 1843 (Digenea, Brachylaimidae) in <em>Sorex> cfr. <em>antinorii> Bonaparte, 1848 (Insectivora, Soricidae) in Italia

    Juan Carlos Casanova; Concetta Milazzo; Gaetano Aloise; Mara Cagnin; Alexis Ribas

    2003-01-01

    Un?indagine parassitologica è stata condotta su esemplari di <em>Sorex> cfr. <em>antinorii> Bonaparte, 1840 (Insectivora, Soricidae) catturati in Sila Grande (Valle Capra; 16.29.30; 39.21.40). Uno degli esemplari è stato trovato parassitato da Trematodi digenei rinvenuti nell?esofago e nello stomaco. I parassiti isolati sono stati fissati con liquido di Bouin, colorati con carminio acetico di Semichon, fissati in una serie di alcooli, chiarificati in xilolo e m...

  8. Functional Expression in <em>Esc>herichia coliem> of the Disulfide-Rich Sea Anemone Peptide APETx2, a Potent Blocker of Acid-Sensing Ion Channel 3

    Glenn F. King

    2012-07-01

    Full Text Available Acid-sensing ion channels (ASICs are proton-gated sodium channels present in the central and peripheral nervous system of chordates. ASIC3 is highly expressed in sensory neurons and plays an important role in inflammatory and ischemic pain. Thus, specific inhibitors of ASIC3 have the potential to be developed as novel analgesics. APETx2, isolated from the sea anemone <em>Anthopleura elegantissimaem>,> em>is the most potent and selective inhibitor of ASIC3-containing channels<em>.> However, the mechanism of action of APETx2 and the molecular basis for its interaction with ASIC3 is not known. In order to assist in characterizing the ASIC3-APETx2 interaction, we developed an efficient and cost-effective <em>Escherichia coliem> periplasmic expression system for the production of APETx2. NMR studies on uniformly 13C/15N-labelled APETx2 produced in <em>E. coliem> showed that the recombinant peptide adopts the native conformation. Recombinant APETx2 is equipotent with synthetic APETx2 at inhibiting ASIC3 channels expressed in <em>Xenopus> oocytes. Using this system we mutated Phe15 to Ala, which caused a profound loss of APETx2’s activity on ASIC3. These findings suggest that this expression system can be used to produce mutant versions of APETx2 in order to facilitate structure-activity relationship studies.

  9. Ticks (Acari: Ixodida: Argasidae, Ixodidae infesting humans in Northwestern Córdoba province, Argentina Garrapatas infestando humanos en el noroeste de la provincia de Córdoba, Argentina

    Nava Santiago

    2006-06-01

    Full Text Available Ticks infesting humans were collected from September 2004 to August 2005 in Northwestern Córdoba in an area with a southern limit in the locality of Dean Funes (30°25´S 64°20´W and San José de las Salinas (30°00´S 64°37´W in the North. The collections consisted in ticks found attached on man obtained from three sources: 1 specimens fixed on two workers during two successive days per month of field work in the northern part of the area which belongs to Western Chaco district of the phytogeographical Chaco domain, 2 ticks attached to a man working in a farm close to Dean Funes in the Chaco Serrano district of the Chaco domain and, 3 ticks collected from a collaborator visiting daily a suburban property with dogsin the vicinities of Dean Funes. Most ticks collected were larvae, nymphs and adults of Amblyomma neumanni from the Chaco Serrano district where a nymph of Otobius megnini was also found on man. Adults of Amblyomma parvum and Amblyomma tigrinum were detected feeding on humans in the Western Chaco district and in the property close to Dean Funes, respectively. Amblyomma neumanni was absent on man from December to April while most specimens of A. parvum and A. tigrinum were collected during summer. Their role as potential vector of ticktransmitted diseases in the area is unknown.Se recolectaron garrapatas (Acari: Ixodida: Argasidae, Ixodidae infestando humanos entre septiembre de 2004 y agosto de 2005 en un área del noroeste de Córdoba cuyo límite al sur es la localidad Deán Funes (30º25´S 64°20´W y el límite al norte es la localidad de San José de las Salinas (30°00´S 64°37´W. Las colecciones consistieron en garrapatas fijadas a humanos obtenidas de tres fuentes: 1 garrapatas fijadas sobre dos trabajadores durante dos días sucesivos por mes de trabajo de campo en la parte norte del área, la cual pertenece al distrito chaqueño occidental del dominio fitogeográfico del Chaco, 2 garrapatas fijadas a un trabajador en

  10. Adaptabilidade e estabilidade em aveia em ambientes estratificados

    Bertan Ivandro; Silva Giovani Olegário da; Valério Igor Pires; Floss Elmar Luíz; Lorencetti Claudir; Vieira Eduardo Alano; Coimbra Jefferson Luís Meireles; Carvalho Fernando Irajá Félix de; Oliveira Antônio Costa de; Benin Giovani

    2005-01-01

    Vinte cultivares de aveia (Avena sativa L.) foram avaliados para rendimento de grãos nas safras agrícolas de 2001 e 2002 em nove locais dos estados do Paraná, Rio Grande do Sul e São Paulo, com o objetivo de avaliar as estimativas dos componentes de adaptabilidade e estabilidade do rendimento de grãos em ambientes favoráveis e desfavoráveis em presença e ausência de aplicação de fungicida. Foi empregada a metodologia de EBERHART & RUSSELL (1966). A presença de significância para anos, genótip...

  11. Casca de soja em dietas para coelhos em crescimento

    Ana Carolina Kohlrausch Klinger; Geni Salete Pinto de Toledo; Daniel Prois Eggers; Alexandra Pretto; Michael Chimainski; Leila Picolli da Silva

    2015-01-01

    O presente trabalho teve como objetivo avaliar efeitos da inclusão de casca de soja em substituição ao feno de alfafa sobre o desempenho, características da carcaça e trato gastrointestinal e parâmetros bioquímicos de coelhos em fase de crescimento. Foram utilizados 30 coelhos da raça Nova Zelândia Branca, divididos igualmente em três tratamentos, compostos pela dieta experimental padrão (sem inclusão de casca de soja); e pelas dietas com 50% e 100% de substituição de alfafa por casca de soja...

  12. Chemical Profiles and Identification of Key Compound Caffeine in Marine-Derived Traditional Chinese Medicine <em>Ostreae em>concha>

    Hong-Bing Liu

    2012-05-01

    Full Text Available To compare the chemical differences between the medicinal and<em> em>cultured oyster shells, their chemical profiles were investigated. Using the ultra performance liquid chromatography-electron spraying ionization-mass spectrometry (UPLC-ESI-MS, combined with principal component analysis (PCA and orthogonal projection to latent structures discriminant analysis (OPLS-DA, the discrimination of the chemical characteristics among the medicinal and cultured oyster shells was established. Moreover, the chemometric analysis revealed some potential key compounds. After a large-scale extraction and isolation, one target key compound was unambiguously identified as caffeine (1 based on extensive spectroscopic data analysis (1D and 2D NMR, MS, and UV and comparison with literature data.

  13. PESQUISA EM DIFICULDADES DE COMPREENSÃO EM LEITURA

    Lucilene Bender de Sousa

    2013-01-01

    Full Text Available Neste artigo revisamos importantes pesquisas sobre as dificuldades de compreensão em leitura. Inicialmente, apresentamos a identificação e classificação das dificuldades já mapeadas. Em seguida, mostramos as contribuições de pesquisas experimentais que investigaram habilidades de baixo nível cognitivo e habilidades de alto nível cognitivo envolvidas na compreensão leitora. Por fim, avaliamos as duas tendências de estudos apresentadas e as necessidades brasileiras no que tange a pesquisa e o ensino da leitura.

  14. Low Diversity Bacterial Community and the Trapping Activity of Metabolites from Cultivable Bacteria Species in the Female Reproductive System of the Oriental Fruit Fly, <em>Bactrocera dorsalisem> Hendel (Diptera: Tephritidae

    Hongyu Zhang

    2012-05-01

    Full Text Available Our goal was to identify the bacteria inhabiting the reproductive system of the female oriental fruit fly, <em>Bactrocera dorsalisem> (Hendel, and evaluate the chemotaxis of <em>B>. <em>dorsalis> to the metabolites produced by the bacteria. Based on 16S rRNA-based polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE, 18 operational taxonomic units (OTUs were assigned to the five bacterial classes <em>Betaproteobacteria>, <em>Alphaproteobacteria>, <em>Gammaproteobacteria>, <em>Bacilli> and <em>Actinobacteria>. Nine OTUs were assigned to <em>Gammaproteobacteria>, which was the most highly represented class. <em>Enterobacteriaceae> constituted the dominant family, and within this family, three genera and five species were identified, including <em>Enterobacter sakazakiiem>, <em>Klebsiella> <em>oxytoca>, <em>Klebsiella> <em>pneumoniae,> Raoultellaem> <em>terrigena em>and <em>Enterobacter> <em>amnigenus>. In this set, the first two species were the dominant components, and the latter three species were the minor ones. Finally, we found that the metabolites produced by <em>R.> terrigenaem>, <em>K. oxytocaem> and <em>K.> pneumoniae em>were attractive to the <em>B.> dorsalisem> adults, and in field studies, <em>B.> dorsalisem> adults were most attracted to <em>K.> oxytocaem>. Collectively, our results suggest that the female reproductive system plays an important role in the transfer of enterobacteria from the gut to fruit. Our data may prompt the development of a female-targeted population control strategy for this fly.

  15. Isolation and Characterization of Microsatellite Markers for <em>Passiflora contractaem>

    Loreta B. Freitas

    2012-09-01

    Full Text Available <em>Passiflora contractaem> Vitta (Passifloraceae is an endemic species of the Atlantic Rainforest, one of the most species-rich ecoregions in the world, although extremely endangered. We have developed an enriched microsatellite library in order to fine-scale studies of the genetic structure<em> em>of> P. contractaem>. Twelve pairs of microsatellite primers were designed, and seven loci were successfully amplified and characterized by genotyping two wild populations of <em>P. contractaem>. All seven loci were polymorphic, with an average number of alleles found being 4.8 and 5 per population. The cross-species transferability was tested using sister species <em>Passiflora ovalisem>> em>Vell. Ex Roemer<em>. em>The development of these markers will contribute to the studies of population genetics in <em>P. contractaem> as well as future studies concerning diversity patterns in the Atlantic Rainforest, and may also help to establish strategies for the conservation of this species.

  16. Thermal Studies of Zn(II, Cd(II and Hg(II Complexes of Some <em>N-Alkyl-N>-Phenyl-Dithiocarbamates

    Peter A. Ajibade

    2012-07-01

    Full Text Available The thermal decomposition of Zn(II, Cd(II and Hg(II complexes of <em>N-ethyl-N>-phenyl and <em>N-butyl-N>-phenyl dithiocarbamates have been studied using thermogravimetric analysis (TGA and differential scanning calorimetry (DSC. The products of the decomposition, at two different temperatures, were further characterized by scanning electron microscopy (SEM and energy-dispersive X-ray spectroscopy (EDX. The results show that while the zinc and cadmium complexes undergo decomposition to form metal sulphides, and further undergo oxidation forming metal oxides as final products, the mercury complexes gave unstable volatiles as the final product.

  17. Rural Emergency Medical Services (EMS) and Trauma

    ... View more Rural Emergency Medical Services (EMS) and Trauma Emergency medical services (EMS) providers care for individuals ... hospital be part of the regional and statewide trauma system? Yes. According to Safety in Numbers: Are ...

  18. Densità di Lepre (<em>Lepus europaeusem>) e Silvilago (<em>Sylvilagus floridanusem>) in ambienti planiziali

    Sandro Bertolino; Perrone, A.; Laura Gola

    2003-01-01

    Il silvilago (<em>Sylvilagus floridanusem>) è un leporide d?origine nordamericana, introdotto in Italia a partire dal 1966 a scopo venatorio. Attualmente la specie è diffusa soprattutto in Piemonte e nella parte occidentale della Lombardia. Il silvilago potrebbe interferire con l?autoctona lepre comune (<em>Lepus europaeusem>), anche se i dati al riguardo sono limitati. Nell?autunno-inverno 2002-2003 abbiamo condotto dei censimenti notturni con faro in alcune aree ...

  19. On the taxonomy and distribution of <em>Crocidura cossyrensisem> and <em>Crocidura russulaem> (Insectivora, Soricidae) in Maghreb

    Longino Contoli; Gaetano Aloise

    2001-01-01

    Abstract The paper examines the taxonomic status of North African <em>Crocidura russula Auctorumem>. On the basis of morphological data, the taxon needs to be divided into at least two species: <em>C. russulaem> (Hermann, 1780) from western Europe, coastal Morocco and middle-western Algeria, up to about the Algiers area, and possibly even Sardinia and Ibiza, and another species, distributed in coastal middle-eastern Algeria, Tunisia, P...

  20. KURU MEYVE AKARI Carpoglyphus Lactis (L.) (Acari :Carpoglyphidae)’ İN FARKLI SICAKLIK VE NEM ORTAMLARINDAKİ GELİŞMESİ ÜZERİNE ARAŞTIRMALAR

    GÜLDALI, Begül

    2007-01-01

    Ankara Üniversitesi Ziraat Fakültesi Bitki Koruma bölümünde yürütülen bu çalışmada Carpoglyphus lactis (L.) (Acari :Carpoglyphidae)’ in 18°C , 23°C ve 28 °C sıcaklık değerlerinde ve %65 ve % 80 orantılı nem değeri kombinasyonlarında biyolojik özellikleri incelenmiştir. Akarın gelişme süresi 5- 12 gün arasında değişmektedir. Sıcaklık ve orantılı nem artışıyla birlikte gelişme süresi kısalmıştır. Ergin ömür uzunluğu 11-68 gün arasında değişim göstermiştir. en uzun ergin ömrü 18°C ve % 65 ...

  1. Do sentimento em Florbela Espanca

    Pereira, Custódia de Jesus Gonçalves

    2005-01-01

    The thesis Do Sentimento em Florbela Espanca presents as its main object of study the feelings and emotions displayed in the works of Florbela Espanca, Livro de Mágoas, Livro de Soror Saudade and Charneca em Flor. This theme is then consolidated in four subthemes, which are – Florbela, the Woman, Social and Cultural Framings, the Canon-Law and the Course Books. Thus, we have Florbela Espanca portrayed as an ordinary woman, with her obscure birth, her personal and academic progress. I...

  2. Pobreza e desigualdade em Portugal

    Sabença, Cindy Sofia dos Santos Alves Ribeiro

    2012-01-01

    A conjuntura económica atual levanta questões sobre a condição de vida e bem-estar da sociedade, obrigando a uma reflexão aprofundada sobre a Pobreza e Desigualdade em Portugal. Este estudo tem como objetivo caracterizar a situação portuguesa e apresentar quais os determinantes da Pobreza em Portugal. Para tal é utilizada a base de dados estatísticos do Eurostat não só para a caracterização da sociedade portuguesa num contexto europeu, como também para desenvolver um modelo de estimação linea...

  3. Procedimentos substantivos em auditoria financeira

    Santos, Ana Sofia Pires dos

    2014-01-01

    O presente relatório visa a conclusão do mestrado em Auditoria e Análise Financeira, cujo tema é Auditoria Financeira e procura apresentar as tarefas desenvolvidas por mim, durante o estágio curricular, enquanto assistente de auditoria na empresa Rosa Lopes Gonçalves Mendes & Associados, SROC, Lda., no Entroncamento durante 22 semanas. O relatório é composto por quatro capítulos em que nos primeiros três capítulos faço uma abordagem teórica geral à auditoria financeira, cujo objetivo é a i...

  4. Anorexia e bulimia em odontopediatria

    Bezerra, Fernanda Barros

    2015-01-01

    Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Medicina Dentária Introdução: A procura de um ideal de beleza e a obsessão pela estética imposta pela sociedade moderna em que se vive têm promovido o aumento do número de pessoas com transtornos alimentares, como por exemplo, a anorexia nervosa e a bulimia nervosa. Esses transtornos contribuem para o aumento de pacientes com prejuízos, diretos e indi...

  5. Factores de risco em implantologia

    Sousa, Inês Nunes de

    2015-01-01

    A Implantologia tem ganho popularidade na medicina dentária pelos resultados previsíveis em reabilitações de áreas edêntulas, permitindo minimizar as consequências funcionais, fonéticas e estéticas que advém da perda de dentes através de uma solução fixa que satizfaz os pacientes. Esta é uma revisão da literatura existente sobre factores de risco, fracassos e complicações inerentes ao tratamento com implantes dentários. Categorizam-se os factores de risco em implantologia co...

  6. Evaluation of Antioxidant Activities of Aqueous Extracts and Fractionation of Different Parts of <em>Elsholtzia em>ciliata>

    Yuangang Zu

    2012-05-01

    Full Text Available The aim of this study was to investigate the antioxidant and free-radical scavenging activity of extract and fractions from various parts of <em>Elsholtzia ciliataem>. The inflorescences, leaves, stems and roots of <em>E. ciliataem> were extracted separately and two phenolic component enrichment methods: ethyl acetate-water liquid-liquid extraction and macroporous resin adsorption-desorption, were adopted in this study. The antioxidant activities of water extracts and fractions of <em>E. ciliataem> were examined using different assay model systems <em>in vitroem>. The fraction root E (purified by HPD300 macroporous resin exhibited the highest total phenolics content (497.2 ± 24.9 mg GAE/g, accompanied with the highest antioxidant activity against various antioxidant systems <em>in vitroem> compared to other fractions. On the basis of the results obtained, <em>E. ciliataem> extracts can be used potentially as a ready accessible and valuable bioactive source of natural antioxidants.

  7. Rodent hosts of <em>Maritrema> sp. (Digenea, Microphallidae in Sardinia Island

    Juan Carlos Casanova

    2003-10-01

    Full Text Available Prospections on helminth fauna of rodents were carried out in the Cedrino river (Eastern of Sardinia island. Twelve <em>Rattus rattusem> (L., 1758 and eight <em>Mus domesticusem> (Schwarz & Schwarz, 1943 were captured by Sherman traps. Parasitological study revealed in <em>R. rattusem> the presence of an intestinal helminth belonging to the genus <em>Maritrema> (family Microphallidae. The transmission of this Digenetic Trematode among vertebrates involves the participation of aquatic invertebrate organisms, molluscs and crustacea, acting as intermediate hosts. Dissection of 117 individuals of the Amphipod Crustacea <em>Gammarus italicusem>, collected in the same biotope, allowed the detection of encysted metacercariae. These larvae constitute the infesting stage of the parasite for vertebrates, acting these as definitive hosts ingesting parasitized crustacea as preys. Adults of <em>Maritrema> sp. were also obtained experimentally in the laboratory mice (<em>Mus domesticusem> CD1 strain. These were infested by inoculating them, using gastric probe, metacercarial cysts isolated from <em>G. italicusem>. Post-infection mice dissection was performed at different intervals of time getting adults in various maturity stages. Experimental facts confirm that <em>Maritrema> sp., as it occurs in other trematodes, do not present strict specificity to the definitive host, being able to develop as well in <em>Mus>. In nature, the presence or absence of this digenean in mammals species will be dependent of host ethological factors, mainly related to feeding habitats. This study was partially supported by the ?Comissionat per Recerca i Universitats de la Generalitat de Catalunya? 2001SGR00088.

  8. Adaptabilidade e estabilidade em aveia em ambientes estratificados

    Bertan Ivandro

    2005-01-01

    Full Text Available Vinte cultivares de aveia (Avena sativa L. foram avaliados para rendimento de grãos nas safras agrícolas de 2001 e 2002 em nove locais dos estados do Paraná, Rio Grande do Sul e São Paulo, com o objetivo de avaliar as estimativas dos componentes de adaptabilidade e estabilidade do rendimento de grãos em ambientes favoráveis e desfavoráveis em presença e ausência de aplicação de fungicida. Foi empregada a metodologia de EBERHART & RUSSELL (1966. A presença de significância para anos, genótipos, fungicidas e locais, indicou comportamento diferencial de genótipos frente às variações de ambiente e a aplicação de fungicida, nas condições de ambientes favoráveis e desfavoráveis. A aplicação de fungicida e o favorecimento do ambiente de cultivo afetaram intensamente os parâmetros de adaptabilidade (b1 e estabilidade (s²d i, indicando que estas estimativas devam ser realizadas em ambientes estratificados, apesar de nenhuma constituição genética ter evidenciado o biótipo ideal, conforme preconizado pelo modelo adotado.

  9. A Novel Apoptosis Correlated Molecule: Expression and Characterization of Protein Latcripin-1 from <em>Lentinula em>edodes> C91–3

    Min Huang

    2012-05-01

    Full Text Available An apoptosis correlated molecule—protein Latcripin-1 of <em>Lentinula> edodesem> C91-3—was expressed and characterized in <em>Pichia pastorisem> GS115. The total RNA was obtained from <em>Lentinula edodesem> C91–3. According to the transcriptome, the full-length gene of Latcripin-1 was isolated with 3'-Full Rapid Amplification of cDNA Ends (RACE and 5'-Full RACE methods. The full-length gene was inserted into the secretory expression vector pPIC9K. The protein Latcripin-1 was expressed in <em>Pichia pastorisem> GS115 and analyzed by Sodium Dodecylsulfonate Polyacrylate Gel Electrophoresis (SDS-PAGE and Western blot. The Western blot showed that the protein was expressed successfully. The biological function of protein Latcripin-1 on A549 cells was studied with flow cytometry and the 3-(4,5-Dimethylthiazol-2-yl-2,5-Diphenyl-tetrazolium Bromide (MTT method. The toxic effect of protein Latcripin-1 was detected with the MTT method by co-culturing the characterized protein with chick embryo fibroblasts. The MTT assay results showed that there was a great difference between protein Latcripin-1 groups and the control group (<em>p em>< 0.05. There was no toxic effect of the characterized protein on chick embryo fibroblasts. The flow cytometry showed that there was a significant difference between the protein groups of interest and the control group according to apoptosis function (<em>p em>< 0.05. At the same time, cell ultrastructure observed by transmission electron microscopy supported the results of flow cytometry. The work demonstrates that protein Latcripin-1 can induce apoptosis of human lung cancer cells A549 and brings new insights into and advantages to finding anti-tumor proteins.

  10. Allometric and Isometric variations in the Italian <em>Apodemus sylvaticusem> and <em>Apodemus flavicollisem> with respect to the conditions of allopatry and sympatry / Variazioni allometriche e isometriche in <em>Apodemus sylvaticusem> e <em>Apodemus flavicollisem> italiani, rispetto alle condizioni di allopatria e simpatria

    Giovanni Amori

    1986-12-01

    Full Text Available Abstract In Italy there are two species of <em>Apodemus> (<em>Sylvaemus>: <em>Apodemus sylvaticusem> on the mainland and the main island, and <em>Apodemus flavicollisem> only on the mainland. The trend of some morphometric characters of the skull (incisive foramen length - FI; interorbital breadth = IO; length of palatal bridge = PP; upper alveolar length = $M^1M^3$ was analized and some theoretical models verified for <em>A. sylvaticusem>. If one considers the sympatric population of <em>A. sylvaticusem> and <em>A. flavicollisem> simultaneously the characters PP, IO and $M^1M^3$ appear significantly isometric being directly correlated ($P leq O.O1$, while FI character results allometric with respect to the previous ones, as expected. If one considers the sympatric populations of each of the species separately, the scenario is different. For <em>A. sylvaticusem> only PP and $M^1M^3$ are isometric ($P leq 0.05$. For <em>A. flavicollisem> only $M^1M^3$ and FI appear to be correlated, although not as significantly as for <em>A. sylvaticusem> ($P le 0.05$; one tail. The insular populations of <em>A. sylvaticusem> do not show significant correlations, except for FI and $M^1M^3$ ($P le 0.05$. On the contrary, considering all populations, sympatric and allopatric, of <em>A. sylvaticusem> at the same time are significant correlations ($P le 0.05$ in all combinations of characters, except for those involving the IO. We suggest that the isometric relations in sympatric assemblages are confined within a morphological range available to the genus <em>Apodemus>. In such a space, the two species are split in two different and innerly homogeneous distributions. We found no evidence to confirm the niche variation hypothesis. On the contrary, the variability expressed as SO or CV's appears higher in the sympatric populations than in the allopatric ones, for three of the four characters, confirming previous results

  11. Parasitic zoonoses: survey in foxes (<em>Vulpes vulpesem> in the northern Apennines / Zoonosi parassitarie: indagini in volpi (<em>Vulpes vulpesem> dell'Appennino settentrionale

    Vittorio Guberti

    1991-07-01

    Full Text Available Abstract A parasitological survey on 153 foxes was carried out in the northern Apennines, during the period 1984-1987. The following parasites were identified: <em>Toxocara canisem> (46.4%, <em>Taenia> sp. (17%, <em>Uncinaria stenocephalaem> (11.8%, <em>Mesocestoides lineatusem> (11.1%, <em>Ancylostoma caninumem> (3.9%, <em>Taenia hydatigenaem> (3.3%, <em>Trichuris vulpisem> (3.3%, <em>Dipylidium caninumem> (2.6%, <em>Taenia crassicepsem> (2%. All foxes were negative for <em>Trichinella> sp. A statistical analysis was performed to evaluate differences in the parasitic fauna according to the sex and age classes of the hosts. The role that the fox could have as a reservoir of helminthic zoonoses is discussed. The results are compared with those of similar studies carried out in Italy. Riassunto Nel periodo 1984-1987 è stata condotta un'indagine parassitologica su 153 volpi abbattute nell'Appennino romagnolo. Sono stati reperiti i seguenti parassiti: <em>Toxocara canisem> (46,4%, <em>Taenia> sp. (17%, <em>Uncinaria stenocephalaem> (11,8%, <em>Mesocestoides lineatusem> (11,1%, <em>Ancylostoma caninumem> (3,9%, <em>Taenia hydatigenaem> (3,3%, <em>Trichuris vulpisem> (3,3%, <em>Dipylidium caninumem> (2,6%, <em>Taenia crassicepsem> (2%. Tutte le volpi esaminate sono risultate negative per <em>Trichinella> sp. È stata effettuata l'analisi statistica dei dati per evidenziare eventuali differenze della fauna parassitaria in relazione al sesso e all'età delle volpi. Sulla base dei dati ottenuti viene discussa l'importanza che la Volpe può assumere come serbatoio di zoonosi elmintiche. I risultati acquisiti sono inoltre comparati con quelli ottenuti in analoghe ricerche condotte in Italia.

  12. Allozymic and biometric variation in <em>Dryomys nitedulaem> (Pallas, 1778

    Maria Grazia Filippucci

    1995-05-01

    Full Text Available Abstract Biometric and genetic variation were analysed among populations of <em>D. nitedulaem> from Italy, Balkan Peninsula, Asia Minor and Israel. The biometric analysis was carried out on 84 specimens using 13 linear skull measurements. Variations in metrical characters among samples were studied by standard and multivariate analyses. The genetic analysis was carried out on 29 specimens by electrophoresis of 40 gene loci. In both analyses the Israeli sample was the most distinct. Skulls of Israeli specimens are characterized by longer mandibular tooth row, higher rhamus mandibulae, longer bullae, and narrower braincase breadth, rostra1 breadth and interorbital constriction. Electrophoretically the Israeli sample can be discriminated from European populations by four loci (<em>Ldh-1em>, <em>G6pdhem>, <em>Pep-1em>, and <em>Lap-2em> fixed for new alleles. The mean value of Nei's genetic distance between Israeli and European populations was <em>D> = 0.186, ranging from 0.153 to 0.227. The Israeli population, commonly attributed to the taxon <em>phrygius>, was different from this by biometric analysis. <em>D. n. phrygiusem> from the topotype locality in Asia Minor clustered with the European population from Turkish Thrace. In order to clarify the phylogenetic relationships of the Israeli forest dormouse, a comparison with other populations from the Caucasus and Iran and with <em>Dryomys lanigerem> from Turkey is suggested. Riassunto Variabilità genetica e biometrica in <em>Dryomys nitedulaem> (Pallas, 1778 - Variabilità biometrica e genetica sono state analizzate tra popolazioni di <em>D. nitetulaem> provenienti da Italia, Penisola Balcanica, Asia Minore ed Israele. L'analisi biometrica è stata condotta su 84 esemplari mediante 13 misurazioni craniche lineari. La variazione nei caratteri metrici tra i campioni è stata studiata mediante analisi standard e multivariata

  13. First sign of <em>Clethrionomys glareolusem> in the Po valley plain / Prima segnalazione in Pianura Padana di <em>Clethrionomys glareolusem>

    Luis Nieder

    1987-07-01

    Full Text Available Abstract The presence of <em>Clethrionomys glareolusem> (Schreber, 1790 populations is reported in the Po valley, formerly considered outside the distribution area of the species. An initial series of captures was made in the high-water area of the Po near S. Nazzaro (Monticelli d'Ongina - PC following with research was extended along the river's banks in Emilia both upstream and downstream. The search for this species was carried out by analysing food pellets relating to <em>Strix alucoem>, <em>Tyto albaem> e <em>Athene noctuaem>, thought to be among the most common predators of these intensive herbaceous and arboreal agricultural biocoenosis. The information we gathered was integrated with other Authors' unpublished and bibliographical data. The presence of <em>C. glareolusem> was noted in habitats quite different from the expected ones. Many of the problems raised by the presence of this species in "islands" along the main body of the Po river and its tributaries, and by its presumed absence from particulary suitable environments such as the Carrega woods, the Ticino park and Mesola wood must still be resolved. Riassunto Viene segnalata la presenza di popolazioni di <em>Clethrionomys glareolusem> (Schreber 1790 nella pianura Padana precedentemente considerata al di fuori dell'areale della specie. A partire da una prima serie di catture nell'area golenale del Po presso S. Nazzaro (Monticelli d'Ongina - PC, si è successivamente proceduto ad allargare l'area di ricerca lungo l'argine emiliano del Po, sia a monte sia a valle. La ricerca di questa specie è avvenuta tramite l'analisi di boli alimentari relativi a <em>Strix alucoem>, <em>Tyto albaem> e <em>Athene noctuaem>, ritenuti tra i più comuni predatori di queste biocenosi agricole intensive erbacee ed arboree. I dati da noi raccolti sono stati integrati con quelli bibliografici e inediti di altri Autori. È stata riscontrata la presenza

  14. Cuidados paliativos em cuidados intensivos

    Fonseca, Sandrina Fernandes de Andrade

    2012-01-01

    Relatório de prática clínica apresentado à Escola Superior de Saúde Dr. Lopes Dias do Instituto Politécnico de Castelo Branco para cumprimento dos requisitos necessários à obtenção do grau de mestre em Cuidados Paliativos

  15. Action of Chitosan Against <em>Xanthomonas> Pathogenic Bacteria Isolated from <em>Euphorbia pulcherrimaem>

    Yanli Wang

    2012-06-01

    Full Text Available The antibacterial activity and mechanism of two kinds of chitosan were investigated against twelve <em>Xanthomonas> strains recovered from <em>Euphorbia pulcherrimaem>. Results indicated that both chitosans markedly inhibited bacterial growth based on OD loss. Furthermore, the release of DNA and RNA from three selected strains was increased by both chitosans. However, the release of intracellular proteins was inhibited by both chitosans at different concentration and incubation times, except chitosan A at 0.1 mg/mL for 0.5 h incubation and 0.2 mg/mL for 2.0 h incubation increased the release of proteins, indicating the complexity of the interaction and cell membranes, which was affected by incubation time, bacterial species, chitosan type and concentration. Transmission electron microscopic observations revealed that chitosan caused changes in protoplast concentration and surface morphology. In some cells, the membranes and walls were badly distorted and disrupted, while other cells were enveloped by a thick and compact ribbon-like layer. The contrary influence on cell morphology may explain the differential effect in the release of material. In addition, scanning electron microscope and biofilm formation test revealed that both chitosans removed biofilm biomass. Overall, this study showed that membrane and biofilm play an important role in the antibacterial mechanism of chitosan.

  16. Preparation and Characterization of <em>O>-Acylated Fucosylated Chondroitin Sulfate from Sea Cucumber

    Na Gao

    2012-08-01

    Full Text Available Fucosylated chondroitin sulfate (FuCS, a kind of complex glycosaminoglycan from sea cucumber, has potent anticoagulant activity. In order to understand the relationship between structures and activity, the depolymerized FuCS (dFuCS was chosen to prepare its derivates by selective substitution at OH groups. Its <em>O>-acylation was carried out in a homogeneous way using carboxylic acid anhydrides. The structures of<em> Oem>-acylated derivatives were characterized by NMR. The results indicated that the 4-<em>O>-sulfated fucose residues may be easier to be acylated than the other ones in the sulfated fucose branches. But the<em> Oem>-acylation was always accompanied by the β-elimination, and the degree of elimination was higher as that of acylation was higher. The results of clotting assay indicated that the effect of partial <em>O>-acylation of the dFuCS on their anticoagulant potency was not significant and the <em>O>-acylation of 2-OH groups of 4-<em>O>-sulfated fucose units did not affect the anticoagulant activity.

  17. Wavelet <em>q->Fisher Information for Scaling Signal Analysis

    Joel Trejo-Sanchez

    2012-08-01

    Full Text Available Abstract: This article first introduces the concept of wavelet <em>q>-Fisher information  and then derives a closed-form  expression of this quantifier for scaling signals of parameter α.  It is shown that this information measure appropriately describes the complexities  of scaling signals and provides further analysis flexibility with the parameter<em> qem>. In the limit of <em>q → 1,em> wavelet <em>q>-Fisher information  reduces to the standard wavelet Fisher information  and for <em>q  > 2em> it reverses its behavior. Experimental results on synthesized fGn signals validates the level-shift  detection capabilities of wavelet q-Fisher information. A comparative study also shows that wavelet<em> qem>-Fisher information  locates structural changes in correlated and anti-correlated fGn signals in a way comparable with standard breakpoint location techniques but at a fraction of the time. Finally, the application of this quantifier to H.<em>263 em>encoded video signals is presented.

  18. <em>Ipomoea aquaticaem> Extract Shows Protective Action Against Thioacetamide-Induced Hepatotoxicity

    A. Hamid A. Hadi

    2012-05-01

    Full Text Available In the Indian system of traditional medicine (Ayurveda it is recommended to consume <em>Ipomoea em>aquatica> to mitigate disorders like jaundice. In this study, the protective effects of ethanol extract of <em>I. aquaticaem> against liver damage were evaluated in thioacetamide (TAA-induced chronic hepatotoxicity in rats. There was no sign of toxicity in the acute toxicity study, in which Sprague-Dawley (SD rats were orally fed with <em>I. aquaticaem> (250 and 500 mg/kg for two months along with administration of TAA (i.p injection 200 mg/kg three times a week for two months. The results showed that the treatment of <em>I. aquaticaem> significantly lowered the TAA-induced serum levels of hepatic enzyme markers (ALP, ALT, AST, protein, albumin, bilirubin and prothrombin time. The hepatic content of activities and expressions SOD and CAT that were reduced by TAA were brought back to control levels by the plant extract supplement. Meanwhile, the rise in MDA level in the TAA receiving groups also were significantly reduced by <em>I. aquaticaem> treatment. Histopathology of hepatic tissues by H&E and Masson trichrome stains displayed that <em>I. aquaticaem> has reduced the incidence of liver lesions, including hepatic cells cloudy swelling, infiltration, hepatic necrosis, and fibrous connective tissue proliferation induced by TAA in rats. Therefore, the results of this study show that the protective effect of <em>I. aquaticaem> in TAA-induced liver damage might be contributed to its modulation on detoxification enzymes and its antioxidant and free radical scavenger effects. Moreover, it confirms a scientific basis for the traditional use of <em>I. aquaticaem> for the treatment of liver disorders.

  19. Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from <em>Catharanthus em>roseus> Leaves

    Yuangang Zu

    2012-07-01

    Full Text Available In the present study, an improved method termed negative-pressure cavitation extraction (NPCE followed by reverse phase high-performance liquid chromatography (RP-HPLC was developed for the extraction and quantification of vindoline (VDL, catharanthine (CTR, vincristine (VCR and vinblastine (VLB from <em>Catharanthus roseus em>leaves. The optimized method employed 60-mesh particles, 80% ethanol, a negative pressure of −0.075 MPa, a solid to liquid ratio of 1:20, 30 min of extraction and three extraction cycles. Under these optimized conditions, the extraction yields of VDL, CTR, VCR and VLB are 0.5783, 0.2843, 0.018 and 0.126 mg/g DW, respectively. These extraction yields are equivalent to those from the well-known ultrasonic extraction method and higher than the yields from maceration extraction and heating reflux extraction. Our results suggest that NPCE-RP-HPLC represents an excellent alternative for the extraction and quantification of vinca alkaloids for pilot- and industrial-scale applications.

  20. Cefaleia em salvas: aspectos clinicos e terapeuticos em 26 casos

    Wilson Luiz Sanvito

    1980-12-01

    Full Text Available Vinte e seis casos de cefaléia-em-salvas são estudados do ponto de vista clínico e terapêutico. Nos aspectos clínicos são ressaltados a dramaticidade deste tipo de cefaléia, o nítido predomínio no sexo masculino, a média de idade dos pacientes, a presença de manifestações associadas e os fatores desencadeantes das crises nos períodos suscetíveis. Do ponto de vista terapêutico três esquemas medicamentosos foram utilizados: maleato de metisergida, prednisona e associação de ambas as drogas. Em 88,3% dos casos um resultado significativo foi alcançado, traduzido por melhora ou abolição das crises.

  1. Phenolic Compounds and Antioxidant Activity of Bulb Extracts of Six <em>Lilium> Species Native to China

    Lixin Niu

    2012-08-01

    Full Text Available Lily (<em>Lilium> is used as an important edible and medical plant species with a vague taxonomic classification and a long history in China. Bulbs of six <em>Lilium> species (<em>L. regaleem>,> L. concolorem>,> L. pumilumem>,> L. leucanthumem>,> L. davidii var. unicolorem> and <em>L. lancifoliumem> native to China were investigated with a view to their exploitation as a potential source of natural antioxidants due to their phenolic composition and dietary antioxidant potential. The results showed that all bulb extracts exhibited strong antioxidant activities, which generally correlated positively with the total phenolic contents (r = 0.68 to 0.94, total flavonoid contents (r = 0.51 to 0.89 and total flavanol contents (r = 0.54 to 0.95. High-performance liquid chromatography (HPLC analysis revealed that rutin and kaempferol were the major phenolic components in the extracts. Hierarchical cluster analysis showed that <em>L. regaleem> belonged to the group with high phenolic content and strong antioxidant power. <em>L. concolorem> and <em>L. pumilumem> were arranged in one group characterized by moderate phenolic content and antioxidant capacity, while <em>L. leucanthumem>, <em>L. davidii var. unicolorem> and <em>L. lancifoliumem> were clustered in the third group with low phenolic content and weak antioxidant activity. These strongly suggest that lily bulbs may serve as a potential source of natural antioxidant for food and pharmaceutical applications.

  2. Fatores de risco cardiovascular em alcoolistas em tratamento

    Mayla Cardoso Fernandes Toffolo

    2013-06-01

    Full Text Available OBJETIVO: Avaliar a presença de fatores de risco cardiovascular, com ênfase na hipertensão e na adiposidade corporal, em alcoolistas abstinentes ou não abstinentes em tratamento. MÉTODO: Trata-se de um estudo transversal com 65 pacientes alcoolistas em tratamento no CAP-Sad. O grau de dependência do álcool foi avaliado pelo SADD (Short Alcohol Dependence Data e o uso de outras drogas, pelo ASSIST (Alcohol Smoking and Substance Involvement Screening Test. Foram avaliados o perfil bioquímico e o antropométrico dos usuários. RESULTADOS: Participaram do estudo 42 homens e 23 mulheres. A maioria dos participantes (67,74% apresentou dependência alcoólica grave, com uso de álcool associado principalmente a cigarro (66,15%. A média da circunferência da cintura (CC foi significativamente maior entre os abstinentes, em comparação aos não abstinentes (AB: 88,15 ± 15,95 x NA: 81,04 ± 9,86; p = 0,03. Pacientes abstinentes há mais tempo tiveram maior sobrepeso/obesidade e adiposidade abdominal (CC do que os não abstinentes e abstinentes recentes, com razão de chances de 5,25. Os abstinentes apresentaram razão de chances de 3,38 para %GC acima da média, independente do tempo de abstinência. CONCLUSÃO: Pacientes alcoolistas abstinentes apresentam mais sobrepeso/ obesidade, adiposidade corporal (%GC e abdominal (CC do que os não abstinentes. É importante o acompanhamento multiprofissional no tratamento de alcoolistas com abordagem para fatores de risco cardiovasculares, principalmente evitando o ganho de peso.

  3. Fadiga em plataformas offshore fixas com modelos em elementos finitos

    Roberto Taier

    2002-09-01

    Full Text Available Quatro juntas tubulares de uma plataforma offshore fixa foram modeladas em elementos finitos para o cálculo das vidas à fadiga e comparação com os resultados obtidos através de modelos convencionais em elementos de barras. Foram também cotejados os fatores de concentração de tensões (SCF's, obtidos com base nas formulações paramétricas, com os calculados através dos modelos em elementos finitos. Os objetivos foram averiguar a validade do refinamento do cálculo da fadiga em juntas críticas da plataforma e avaliar as suas conseqüências na elaboração do plano de inspeção. Os resultados permitiram concluir que a análise por elementos finitos torna-se recomendada para juntas que não apresentam tipo e/ou comportamento compatíveis com as configurações padronizadas, normalmente utilizadas pelos programas. Das quatro juntas analisadas, duas delas não necessitariam fazer parte do plano de inspeção da plataforma.Four tubular joints of a fixed offshore platform were modelled using finite elements to evaluate respective fatigue lifes and comparison with the results obtained from conventional models in frame elements. Stress concentration factors (SCF's calculated from parametric formulas were also compared with those obtained from finite elements models. The results of this work have the intention of verifying the validity of the refinement of fatigue analysis on critical joints of the platform and the evaluation of its consequences in the inspection plan. Obtained results show that finite elements analysis is recommended for joints whose type and/or behaviour is not consistent with standard models, used by computational programs. From four analised joints, two of them would not need to be part of inspection plan.

  4. Phytochemical Composition, Antioxidant and Xanthine Oxidase Inhibitory Activities of <em>Amaranthus cruentusem> L. and <em>Amaranthus hybridusem> L. Extracts

    Jeanne F. Millogo

    2012-06-01

    Full Text Available This paper describes a preliminary assessment of the nutraceutical value of <em>Amaranthus cruentus (A. cruentusem> and <em>Amaranthus hybridus (A. hybridusem>, two food plant species found in Burkina Faso. Hydroacetonic (HAE, methanolic (ME, and aqueous extracts (AE from the aerial parts were screened for <em>in vitroem> antioxidant and xanthine oxidase inhibitory activities. Phytochemical analyses revealed the presence of polyphenols, tannins, flavonoids, steroids, terpenoids, saponins and betalains. Hydroacetonic extracts have shown the most diversity for secondary metabolites. The TLC analyses of flavonoids from HAE extracts showed the presence of rutin and other unidentified compounds. The phenolic compound contents of the HAE, ME and AE extracts were determined using the Folin–Ciocalteu method and ranged from 7.55 to 10.18 mg Gallic acid equivalent GAE/100 mg. Tannins, flavonoids, and flavonols ranged from 2.83 to 10.17 mg tannic acid equivalent (TAE/100 mg, 0.37 to 7.06 mg quercetin equivalent (QE /100 mg, and 0.09 to 1.31 mg QE/100 mg, respectively. The betacyanin contents were 40.42 and 6.35 mg Amaranthin Equivalent/100 g aerial parts (dry weight in <em>A. cruentusem> and <em>A. hybridusem>, respectively. Free-radical scavenging activity expressed as IC50 (DPPH method and iron reducing power (FRAP method ranged from 56 to 423 µg/mL and from 2.26 to 2.56 mmol AAE/g, respectively. Xanthine oxidase inhibitory activities of extracts of <em>A. cruentus em>and <em>A. hybridusem> were 3.18% and 38.22%, respectively.<em> em>The> A. hybridusem> extract showed the best antioxidant and xanthine oxidase inhibition activities. The results indicated that the phytochemical contents of the two species justify their traditional uses as nutraceutical food plants.

  5. Damage caused by <em>Skrjabingylus nasicolaem> (Leuckart, 1842, Metastrongylidae, to weasels (<em>Mustela nivalisem> L. in North-Western Italy

    Claudio Prigioni

    1995-12-01

    Full Text Available Abstract The percentage of infestation (incidence and the degree of damage caused by <em>Skrjabingylus nasicolaem> were analysed in 15 fresh skulls of weasels (<em>Mustela nivalisem> collected in Pavia province (Northern Italy during 1986-89 and in 79 preserved skulls from the Civic Museum of Natural History of Genoa (Liguria region. The incidence was 66.6% for the first sample of weasels and 54.4% for the second one; the mean degree of damage, assessed according King's methods, was 3.7 and 2.6 respectively. Although no significant difference in <em>Skrjabingylus> prevalence was recorded in relation to sex and age of weasels, a trend of increase in parasite incidence was observed from young to adults in the sample of weasels from the Museum of Genoa. <em>Apodemus sylvaticusem>, which was the staple in the diet of weasels collected in Pavia province, could be considered the main paratenic host of the skrjabingylosis in northern Italian plain. Riassunto Danni causati da <em>Skrjabingylus nasicolaem> (Leuckart, 1942, Metastrongylidae, nella donnola <em>Mustela nivalisem> in Italia nord occidentale - La percentuale di infestazione (incidenza e l'entità del danno causato da S<em>krjabingylus nasicolaem> sono state analizzate in 15 donnole (<em>Mustela nivalisem> reperite in aree coltivate della Provincia di Pavia nel 1986-89 e in 79 esemplari appartenenti alla collezione del Museo Civico di Storia Naturale di Genova. L'incidenza dell'infestazione è risultata del 66,6% per il primo campione e del 54,4% per il secondo, mentre l'entità media del danno, valutata secondo il metodo proposto da King, è stata di 3,7 e 2,6 rispettivamente. Sebbene non sia stata rilevata nessuna differenza signiticativa della prevalenza parassitaria in relazione al sesso ed all'età delle donnole, è stata evidenziata, per il campione del Museo di Genova, una tendenza all'incremento dell'incidenza dai

  6. Soropositividade para brucelose em suínos em abatedouros

    Diego C. Rosa

    2012-07-01

    Full Text Available O presente trabalho teve por objetivo investigar a soropositividade para brucelose em suínos em abatedouros. Foram coletados 910 soros de suínos, procedentes de 30 propriedades, abatidos em frigoríficos da região central do estado de São Paulo, e submetidos às provas de soroaglutinaçao com antígeno tamponado acidificado (AAT e 2-Mercaptoetanol (2-Me objetivando determinar a ocorrência da enfermidade nesta espécie. Do total de soros avaliados foram observados 25 (2,7% animais reagentes ao AAT pertencentes a 10 propriedades, caracterizando 36% de propriedades positivas. Dos animais positivos ao AAT, 16% apresentaram titulo de 25 (incompleto e 52% titulo de 25 na soroaglutinação lenta (SAL, 8% apresentaram titulo de 50 incompleto na SAL e 25 incompleto no 2-ME e 8% apresentaram titulo de 50 na SAL e 25 no 2-ME. Estes resultados demonstram o elevado percentual de propriedades positivas para brucelose nesta região e ressaltam a necessidade de implementação de programas oficiais efetivos para o controle da brucelose suína.

  7. <em>Hedyotis diffusaem> Willd Inhibits Colorectal Cancer Growth <em>in Vivoem> via Inhibition of STAT3 Signaling Pathway

    Jun Peng

    2012-05-01

    Full Text Available Signal Transducer and Activator of Transcription 3 (STAT3, a common oncogenic mediator, is constitutively activated in many types of human cancers; therefore it is a major focus in the development of novel anti-cancer agents. <em>Hedyotis diffusaem> Willd has been used as a major component in several Chinese medicine formulas for the clinical treatment of colorectal cancer (CRC. However, the precise mechanism of its anti-tumor activity remains largely unclear. Using a CRC mouse xenograft model, in the present study we evaluated the effect of the ethanol extract of<em> Hedyotis diffusaem> Willd (EEHDW on tumor growth <em>in vivoem> and investigated the underlying molecular mechanisms. We found that EEHDW reduced tumor volume and tumor weight, but had no effect on body weight gain in CRC mice, demonstrating that EEHDW can inhibit CRC growth <em>in vivoem> without apparent adverse effect. In addition, EEHDW treatment suppressed STAT3 phosphorylation in tumor tissues, which in turn resulted in the promotion of cancer cell apoptosis and inhibition of proliferation. Moreover, EEHDW treatment altered the expression pattern of several important target genes of the STAT3 signaling pathway, <em>i.e.>, decreased expression of Cyclin D1, CDK4 and Bcl-2 as well as up-regulated p21 and Bax. These results suggest that suppression of the STAT3 pathway might be one of the mechanisms by which EEHDW treats colorectal cancer.

  8. Trabalho em grupo com enlutados

    Melissa Pascoal

    2012-12-01

    Full Text Available A cada dia temos mais famílias vivenciando o luto gerado por homicídios, acidentes e violência, e isso tem causado alguns problemas psicológicos para as pessoas que estão nesta situação. Este artigo é um relato da experiência profissional vivida no Hospital e Maternidade Mauá com um grupo de autoajuda para pacientes em processo de luto, e seu objetivo é compartilhar a experiência e difundir este trabalho. Em 15 encontros pré-estruturados de acordo com a demanda apresentada pelo próprio grupo, foi possível trabalhar diversos temas relacionados à perda que influenciaram as participantes e ao mesmo tempo proporcionaram, além de conforto, uma possibilidade de identificação e troca, e consequentemente o ressurgimento da autoconfiança. Por meio da articulação da teoria com a prática, é evidenciado que o atendimento em grupo para enlutados é uma alternativa viável que trouxe resultados positivos e inspiradores e sua divulgação é importante para ampliar horizontes e criar novas possibilidades.

  9. Trophic systems and chorology: data from shrews, moles and voles of Italy preyed by the barn owl / Sistemi trofici e corologia: dati su <em>Soricidae>, <em>Talpidae> ed <em>Arvicolidae> d'Italia predati da <em>Tyto albaem> (Scopoli 1769

    Longino Contoli

    1986-12-01

    Full Text Available Abstract In small Mammals biogeography, available data are up to now by far too scanty for elucidate the distribution of a lot of taxa, especially with regard to the absence from a given area. In this respect, standardized quantitative sampling techniques, like Owl pellets analysis can enable not only to enhance faunistic knowledges, but also to estimate the actual absence probability of a given taxon "m", lacking from the diet of an individual raptor. For the last purpose, the relevant frequencies of "m" in the other ecologically similar sites of the same raptor species diets are averaged ($f_m$ : the relevant standard error (multiplicated by a coefficient, according to the desired degree of accuracy, in relation of the integral of probabilities subtracted ($overline{F}_m - a E$: then, the probability that a single specimen is not pertaining to "m" is obtained ($P_0 = 1 - F_m + a E$; lastly, the desiderate accuracy probability ($P_d$ is chosen. Now, "$N_d$" (the number of individuals of all prey species in a single site needed for obtain, with the desired probability, a specimen at least of "m" is obtained through $$N = frac{ln P_d}{ln P_0}$$ Obviously, every site-diet with more than "N" preyed individuals and without any "i" specimen is considered to be lacking of such taxon. A "usefulness index" for the above purposes is outlined and checked about three raptors. Some exanples about usefulness of the Owl pellet analysis method in biogeography are given, concerning <em>Tyto albaem> diets in peninsular Italy about: - <em>Sorex minutusem>, lacking in some quite insulated areas; - <em>Sorex araneusem> (sensu stricto, after GRAF et al., 1979, present also in lowland areas in Emilia-Romagna; - <em>Crocidura suaveolensem> and - <em>Suncus etruscusem>, present also in the southermost part of Calabria (Reggio province; - <em>Talpa caecaem>, present also in the Antiapennines of Latium (Cimini mounts; - <em>Talpa romanaem

  10. Meroterpenes from Endophytic Fungus A1 of Mangrove Plant <em>Scyphiphora hydrophyllaceaem>

    Peter Proksch

    2012-09-01

    Full Text Available Four new meroterpenes, guignardones F–I (14, together with two known compounds guignardones A (5 and B (6 were isolated from the endophytic fungus A1 of the mangrove plant <em>Scyphiphora hydrophem>yllacea>. Their structures and relative configurations were elucidated by spectroscopic data and single-crystal X-ray crystallography. A possible biogenetic pathway of compounds 16 was also proposed. All compounds were evaluated for inhibitory activity against methicillin-resistant <em>Staphylococcus aureusem> (MRSA and <em>Staphylococcus aureusem>.

  11. Ultrasound-Assisted Extraction of Carnosic Acid and Rosmarinic Acid Using Ionic Liquid Solution from <em>Rosmarinus officinalisem>>

    Chunjian Zhao

    2012-09-01

    Full Text Available Ionic liquid based, ultrasound-assisted extraction was successfully applied to the extraction of phenolcarboxylic acids, carnosic acid and rosmarinic acid, from <em>Rosmarinus officinalisem>. Eight ionic liquids, with different cations and anions, were investigated in this work and [C8mim]Br was selected as the optimal solvent. Ultrasound extraction parameters, including soaking time, solid–liquid ratio, ultrasound power and time, and the number of extraction cycles, were discussed by single factor experiments and the main influence factors were optimized by response surface methodology. The proposed approach was demonstrated as having higher efficiency, shorter extraction time and as a new alternative for the extraction of carnosic acid and rosmarinic acid from <em>R. officinalisem>> em>compared with traditional reference extraction methods. Ionic liquids are considered to be green solvents, in the ultrasound-assisted extraction of key chemicals from medicinal plants, and show great potential.

  12. Morphological identification of the Soprano Pipistrelle (<em>Pipistrellus pygmaeusem> Leach, 1825 in Croatia.

    Igor Pavlinić

    2008-07-01

    Full Text Available Abstract After the discovery of two different phonic types within the common pipistrelle (<em>Pipistrellus pipistrellusem>, mtDNA analysis confirmed the existence of two separate species named as common pipistrelle (<em>P. pipistrellusem> and soprano pipistrelle (<em>P. pygmaeusem>. The discrimination of these two cryptic species using external characters and measures has proved to be somewhat problematic. We examined two colonies of soprano pipistrelle from Donji Miholjac, Croatia. As a result, only two characters proved to be of help for field identification: wing venation (89% of cases and penis morphology and colour for males. The difference in length between the 2nd and 3rd phalanxes of the 3rd finger should be discarded as diagnostic trait between <em>P. pipistrellusem> and <em>P. pygmaeusem> in Croatia. Riassunto Identificazione su basi morfologiche del pipistrello pigmeo (<em>Pipistrellus pygmeausem>, Leach, 1825 in Croazia. A seguito della descrizione di due differenti "tipi fonici" nel pipistrello nano (<em>Pipistrellus pipistrellusem> e della successiva conferma su basi genetiche dell'esistenza di due specie distinte, designate come pipistrello nano (<em>P. pipistrellusem> e pipistrello pigmeo (<em>P. pygmaeusem>, la distinzione delle due specie in base a caratteristiche morfologiche esterne si è dimostrata un problema di difficile soluzione. Sulla base delle caratteristiche distintive e delle differenze biometriche proposte da altri Autori, sono state esaminate due colonie di pipistrello pigmeo a Donji Miholjac, in Croazia. I risultati ottenuti evidenziano che, tra tutti i potenziali caratteri sinora proposti, solo due risultano utili per un'identificazione diretta sul campo: la venatura delle ali, risultata utile alla discriminazione nell'89% degli esemplari analizzati, e la colorazione e morfologia del pene nei maschi. La

  13. Helminth communities of wood mouse (<em>Apodemus sylvaticusem> on the river Avena (Calabria, southern Italy

    Concetta Milazzo

    2011-02-01

    Full Text Available Helminth parasites of woodmouse <em>Apodemus sylvaticusem> were studied from May 1993 through to June 1994 on the River Avena (Calabria, southern Italy, which, as other Mediterranenan rivers, is dry for most of the year. Trapping sites were located in three different habitats of the river: slopes, shores and the riverbed. A total of 106 animals was captured and screened for the presence of helminths. Five endoparasitic species were detected: <em>Corrigia vittaem>, <em>Rictularia proniem>, <em>Heligmosomoides polygyrusem>, <em>Syphacia stromaem> and <em>S. fredericiem>. Four helminth species were recorded from the riverbed and shore sections, whereas only <em>H. polygyrusem> and <em>S. fredericiem> were found on the slopes. <em>Syphacia fredericiem> occurred at every trapping site. The total prevalence was 25.5% and total mean intensity was 27.2 parasites/mouse. No statistical significant difference was found for both the prevalence and mean intensity of infection between either habitats or sexes, suggesting that, from the point of view of the helminth community, the study area should be considered as a single habitat.

  14. Briareolate Esters from the Gorgonian <em>Briareum asbestinumem>

    Lyndon M. West

    2012-08-01

    Full Text Available Two new briarane diterpenoids briareolate esters J (1 and K (2 were isolated from the methanolic extract of the octocoral <em>Briareum> asbestinum em>collected off the coast of Boca Raton, Florida. The structures of briaranes 1 and 2 were elucidated by interpretation of spectroscopic data. Briareolate ester K (2 showed weak growth inhibition activity against human embryonic stem cells (BG02.

  15. Feeding habits of the genet <em>Genetta genettaem> in an Iberian continental wetland

    Maite Sánchez; Patricia Rodrigues; Vicente Ortuño; Juan Herrero

    2009-01-01

    Abstract This study quantified the diet of the genet <em>Genetta genettaem> in an Iberian continental wetland, the Galachos Nature Reserve, northern Spain, based on the frequency with which items appeared in five monthly-surveyed latrines during 2004-2005. Prey types were identified <em>de visuem> using identification keys and expert advice. the genet was confirmed as an opportunistic and generalist predator, its diet including mam...

  16. Butylated Hydroxytoluene Analogs: Synthesis and Evaluation of Their <em>Multipotent em>Antioxidant Activities

    Wageeh A. Yehye

    2012-06-01

    Full Text Available A computer-aided predictions of antioxidant activities were performed with the Prediction Activity Spectra of Substances (PASS program. Antioxidant activity of compounds 1, 3, 4 and 5 were studied using 1,1-diphenyl-2-picrylhydrazyl (DPPH and lipid peroxidation assays to verify the predictions obtained by the PASS program. Compounds 3 and 5 showed more inhibition of DPPH stable free radical at 10−4 M than the well-known standard antioxidant, butylated hydroxytoluene (BHT. Compound 5 exhibited promising <em>in> <em>vitro> inhibition of Fe2+-induced <em>lipid peroxidationem> of the essential egg yolk as a lipid-rich medium<em> em>(83.99%, IC50 16.07 ± 3.51 µM/mL compared to a-tocopherol (a-TOH, 84.6%, IC50 5.6 ± 1.09 µM/mL. The parameters for drug-likeness of these BHT analogues were also evaluated according to the Lipinski’s “rule-of-five” (RO5. All the BHT analogues were found to violate one of the Lipinski’s parameters (Log<em>P> > 5, even though they have been found to be soluble in protic solvents. The predictive polar surface area (PSA and absorption percent (% ABS data allow us to conclude that they could have a good capacity for penetrating cell membranes. Therefore, one can propose these new multipotent antioxidants (MPAOs as potential antioxidants for tackling <em>oxidative stressem> and <em>lipid peroxidation processes.em>> em>

  17. Imaginary Jews and True Confessions: Ethnicity, Lyricism, and John Berryman's <em>Dream Songsem>

    Andrew S. Gross

    2009-01-01

    Berryman was fascinated with the figure of "the imaginary Jew." The phrase is the title of his first short story, it recurs in <em>The Dream Songsem>, and it was to have been the topic of the final chapter of his autobiographical novel <em>Recovery>. Critics have not treated Berryman's "imaginary Jew" kindly. Early critics saw prosopopoeia as uncongenial to the confessional project. More recent critics see the figure as a misappropriation of Jewish ident...

  18. Saponins with Neuroprotective Effects from the Roots of <em>Pulsatilla cernuaem>

    Wen-Hao Fan; Zong-Gui Yu; Hui Zhang; Yong-Nan Xu; Li-Bo Zou; Yi-Xia Gong; Hui-Ming Hua; Jian-Yu Liu; Ying-Li Guan

    2012-01-01

    Four new oleanene-type triterpenoid saponins together with six known saponins were isolated from the roots of <em>P>ulsatilla cernuaem> and their structures were elucidated on the basis of spectroscopic data, including 2D NMR spectra and chemical evidence. Among these one of the aglycones (gypsogenin) is reported for the first time from this genus. Some of these compounds showed significant neuroprotective effects against the

  19. Karyotypes of <em>Nannospalax> (Palmer 1903) populations (Rodentia: Spalacidae) from centraleastern Anatolia, Turkey

    Yüksel Coskun; Servet Ulutürk; Alaettin Kaya

    2010-01-01

    Riassunto
    Cariotipi di <em>Nannospalax> (Palmer 1903) (Rodentia, Spalacidae) dell’Anatolia centro-orientale, Turchia
    Sono stati analizzati i cariotipi di 20 (11 maschi e 9 femmine) <em>Nannospalax> catturati, nel periodo 2006-2009, in 11 località dell’Anatolia centro-orientale (Turchia). Sono state individuate sei diverse forme cromosomiche (2n=49; 2...

  20. Free Radical Scavenging, Antimicrobial and Immunomodulatory Activities of <em>Orthosiphon stamineusem>

    Nabil S. Harmal

    2012-05-01

    Full Text Available <em>Orthosiphon stamineus em>is considered an important traditional folk medicine. In this study ethanol and aqueous extracts of <em>O. stamineusem> were evaluated<em> in vitro em>for their antioxidant, antimicrobial as well as for their immunomodulatory properties on human peripheral blood mononuclear cells (PBMCs. The DPPH radical scavenging method was used for the determination of antioxidant activity, while the antibacterial efficacy was investigated by both disc diffusion method and Minimum Inhibitory Concentration (MIC against four bacterial strains (Gram-positive and Gram-negative. Furthermore, the immunomodulatory potential of the extracts was investigated through the MTT assay. Aqueous extract of <em>O. stamineusem> exhibited significant free radical scavenging activity with IC50 9.6 µg/mL, whereas the IC50 for the ethanol extract was 21.4 µg/mL. The best antimicrobial activity was shown by the aqueous extract of <em>O. stamineus em>against <em>Staphylococcus aureusem>, with inhibition zone of 10.5 mm and MIC value 1.56 mg/mL. Moreover, the results observed from the MTT assay showed that both plant extracts stimulated the PBMCs proliferation<em> in vitroem> in a concentration-dependent manner, but the aqueous extract has remarkable activity against PBMCs. These findings indicate that <em>O. stamineusem> showed high antioxidant activity and may be considered as an immunomodulatory agent.

  1. Variabilità morfologica ed ecologica in <em>Neomys fodiensem> e <em>Neomys anomalusem> nell'Appennino settentrionale

    Dino Scaravelli

    2003-10-01

    Full Text Available I due <em>Neomys> italiani sono ancora da chiarire dal punto di vista della loro caratterizzazione morfologica e ecologica. Il lavoro prende in considerazione un campione di entrambe le specie proveniente da habitat forestali dell?Appennino settentrionale per i quali sono stati identificati i principali parametri ambientali. Vengono quindi descritte la variabilità dei tratti morfologici delle due specie in aree localizzate nel Parco Nazionale Foreste Casentinesi, Monte Falterona e Campigna nell?Appennino tosco-romagnolo. Risultano di sicuro effetto discriminatorio la maschera facciale, il rapporto piede posteriore/coda e i caratteri cranici. Sulla base dei criteri identificativi si sono realizzate rilevazioni di misure corporee per le due specie e una comparazione degli habitat utilizzati. <em>N. fodiensem> appare unica specie nelle faggete-abetine e dominante nei castagneti, mentre nell?Ontaneta e nelle zone aperte e termofile si registra la sola presenza di <em>N. anomalusem>. Mancano entrambi nei prati cespugliati, nella pecceta e nella cerreta. I gradienti presenti sono quindi illustrati. Non appare una differenza altitudinale nel campione esaminato, posto in stazioni tra i 400 e i 1300 m, ma per entrambe vi sono maggiori riscontri nella fascia tra 700 e 850 m. Nell?analisi multivariata rispetto alle altre specie e alle variabili ambientali si riscontra sempre una discreta correlazione con la presenza di acqua di una certa ampiezza, che comunque è significativa solo per <em>N. fodiensem>, mentre risulta di interesse la positiva correlazione di <em>N. anomalusem> con <em>Apodemus sylvaticusem>.

  2. Atividade de glutationa S-transferase na metabolização de acetochlor, atrazine e oxyfluorfen em milho (<em>Zea maysem> L.), sorgo (<em>Sorghum bicolorem> L.) e trigo (<em>Triticum aestivumem> L.) (Poaceae) Glutathione S-transferase activity in acetochlor, atrazine and oxyfluorfen metabolization in maize (<em>Zea maysem> L.), sorghum (<em>Sorghum bicolorem> L.) and wheat (<em>Triticum aestivumem>L.) (Poaceae)

    Ethel Lourenzi Barbosa Novelli; José Claudionir Carvalho; Guilherme Fernando Gomes Déstro; Karina Luiz Chamma; Leonardo César Ferreira; Ana Catarina Cataneo

    2002-01-01

    Este experimento foi conduzido para avaliar a seletividade em plantas dos herbicidas acetochlor, atrazine e oxyfluorfen em relação à atividade da glutationa S-transferase (GST) em plantas de milho (Zea mays L.), sorgo (Sorghum bicolor L.) e trigo (Triticum aestivum L.) (Poaceae). A atividade da GST foi detectada às 24, 48 e 72 horas após as aplicaç��es dos tratamentos. Os tratamentos do experimento consistiram de aplicação com água (controle), acetochlor (3 L.ha-1), atrazine (4 L.ha-1) e oxyf...

  3. Detection of protozoans Babesia microti and [i]Toxoplasma gondii[/i] and their co-existence in ticks (Acari: Ixodida collected in Tarnogórski district (Upper Silesia, Poland

    Marek Asman

    2015-02-01

    Full Text Available [b]Introduction[/b]. Ticks (Acari: Ixodida are vectors of many pathogens i. a: [i]Babesia microti [/i][Bm] and [i]Toxoplasma gondii[/i] [Tg]. In Poland, Ixodes ricinus [Ir] is the main vector of both pathogens. This tick species and pathogens transmitted by them are a significant threat to human and animal health. objectives of the study. Detection of the protozoans Bm and Tg in ticks collected in the Tarnogórski district area. [b]Material and methods[/b]. The ticks were collected from vegetation and pets in the spring period of their activity on the territory of the Tarnogórski district. The parasites were preserved in 70% ethanol. DNA was isolated by ammonia method. Bm was detected by nested-PCR using specific primers for the 18S rRNA sequence. To detect the Tg a commercial kit was used. The PCR products were separated on 2% ethidium bromide stained agarose gels and visualised under UV light. [b]Results[/b]. It was showed that all collected ticks belonged to the species Ir. Bm was detected in 50.87% and Tg in 64.91% of all examined ticks. Co-existence of these both protozoans in 36.84% of total examined ticks was noted. [b]Conclusions[/b]. The study showed a high risk of exposure to Bm and Tg in the studied area. Ticks Ir play an important role in the transmission of Bm in this region. Demonstrating a high percentage of ticks collected from animals infected with Tg may indicate their important role in the transmission of this pathogen, but it requires a further studies.

  4. Encapsulation-Induced Stress Helps <em>Saccharomyces cerevisiae em>Resist Convertible Lignocellulose Derived Inhibitors

    Johan O. Westman

    2012-09-01

    Full Text Available The ability of macroencapsulated <em>Saccharomyces cerevisiae em>CBS8066<em> em>to withstand readily and not readily <em>in situem> convertible lignocellulose-derived inhibitors was investigated in anaerobic batch cultivations. It was shown that encapsulation increased the tolerance against readily convertible furan aldehyde inhibitors and to dilute acid spruce hydrolysate, but not to organic acid inhibitors that cannot be metabolized anaerobically. Gene expression analysis showed that the protective effect arising from the encapsulation is evident also on the transcriptome level, as the expression of the stress-related genes <em>YAP1em>, <em>ATR1em> and <em>FLR1em> was induced upon encapsulation. The transcript levels were increased due to encapsulation already in the medium without added inhibitors, indicating that the cells sensed low stress level arising from the encapsulation itself. We present a model, where the stress response is induced by nutrient limitation, that this helps the cells to cope with the increased stress added by a toxic medium, and that superficial cells in the capsules degrade convertible inhibitors, alleviating the inhibition for the cells deeper in the capsule.

  5. Damage caused by <em>Skrjabingylus nasicolaem> (Leuckart, 1842), Metastrongylidae, to weasels (<em>Mustela nivalisem> L.) in North-Western Italy

    Claudio Prigioni; Anna Boria

    1995-01-01

    Abstract The percentage of infestation (incidence) and the degree of damage caused by <em>Skrjabingylus nasicolaem> were analysed in 15 fresh skulls of weasels (<em>Mustela nivalisem>) collected in Pavia province (Northern Italy) during 1986-89 and in 79 preserved skulls from the Civic Museum of Natural History of Genoa (Liguria region). The incidence was 66.6% for the first sample of weasels and 54.4% for the second one; the mean degr...

  6. Lectin from <em>Canavalia brasiliensisem> Seeds (ConBr Is a Valuable Biotechnological Tool to Stimulate the Growth of <em>Rhizobium tropiciem> <em>in Vitroem>

    Ricardo Pires dos Santos

    2012-05-01

    Full Text Available To study the interactions between a <em>Rhizobium tropiciem> strain and lectins isolated from the seeds of <em>Canavalia ensiformisem> (ConA and <em>Canavalia brasiliensisem> (ConBr, a lectin fluorescence assay was performed. In addition, an experiment was designed to evaluate the effect of the two lectins on bacterial growth. Both lectins were found to bind to <em>R. tropiciem> cells, but the interactions were inhibited by D-mannose. Interestingly, only ConBr stimulated bacterial growth in proportion to the concentrations used (15.6–500 µg/mL, and the bacterial growth stimulation was inhibited by D-mannose as well. Structure/Function analyses by bioinformatics were carried out to evaluate the volume and carbohydrate recognition domain (CRD configuration of ConA and ConBr. The difference of spatial arrangement and volume of CRD may indicate the variation between biological activities of both lectins. The results suggest that ConBr could be a promising tool for studies focusing on the interactions between rhizobia and host plants.

  7. Altitudinal distribution of the common longeared bat <em>Plecotus auritusem> (Linnaeus, 1758 and grey long-eared bat <em>Plecotus austriacusem> (J. B. Fischer, 1829 (Chiroptera, Vespertilionidae in the Tatra mountains (southern Poland

    Krzysztof Piksa

    2006-03-01

    Full Text Available Riassunto Distribuzione altitudinale di Orecchione bruno (<em>Plecotus auritusem> e Orecchione meridionale (<em>Plecotus austriacusem> nei Monti Tatra (Polonia meridionale. Vengono riportati nuovi dati relativi alla distribuzione altitudinale nei Monti Tatra (Polonia meridionale di <em>Plecotus auritusem> e <em>P. austriacusem>. Tali segnalazioni incrementano le conoscenze relative alla presenza di questi chirotteri a quote elevate, in particolare per la Polonia. In inverno <em>P. auritusem> è stato rinvenuto a 1921 m s.l.m. mentre in estate è stato rinvenuto a 2250 m s.l.m.; in aggiunta, sono stati ritrovati resti ossei a 1929 m s.l.m. <em>P. austriacusem> è stato segnalato in ibernazione a 1294 m s.l.m.

  8. Isolation and Characterization of 46 Novel Polymorphic EST-Simple Sequence Repeats (SSR Markers in Two Sinipercine Fishes<em> em>(Siniperca> and Cross-Species Amplification

    Liang Cao

    2012-07-01

    Full Text Available With the development of next generation sequencing technologies, transcriptome level sequence collections are emerging as prominent resources for the discovery of gene-based molecular markers. In this study, we described the isolation and characterization of 46 novel polymorphic microsatellite loci for <em>Siniperca chuatsiem> and <em>Siniperca scherzeriem> from the transcriptome of their F1 interspecies hybrids. Forty-three of these loci were polymorphic in <em>S. chuatsiem>, and 20 were polymorphic in <em>S. scherzeriem>. In <em>S. chuatsiem>, the number of alleles per locus ranged from 2 to 8, and the observed and expected heterozygosities varied from 0.13 to 1.00 and from 0.33 to 0.85, respectively. In <em>S. scherzeriem>, the number of alleles per locus ranged from 3 to 9, and the observed and expected heterozygosities varied from 0.19 to 1.00 and from 0.28 to 0.88, respectively. We also evaluated the cross-amplification of 46 polymorphic loci in four species of sinipercine fishes: <em>Siniperca kneriem>, <em>Siniperca undulataem>, <em>Siniperca obscuraem>, and <em>Coreoperca whiteheadiem>. The interspecies cross-amplification rate was very high, totaling 94% of the 184 locus/taxon combinations tested. These markers will be a valuable resource for population genetic studies in sinipercine fishes.

  9. OCCURENCE OF Salmonella IN CHICKEN CARCASSES AND CUTS COMMERCIALIZED IN GOIÂNIA-GO OCORRÊNCIA DE Salmonella EM CARCAÇAS E CORTES DE FRANGOS COMERCIALIZADOS EM GOIÂNIA-GO

    Iolanda Aparecida Nunes

    2007-09-01

    Full Text Available

    Its was analised 53 chicken carcasses and commercial cuts acquired in supermarkets of Goiânia-GO aiming to isolate <em>Salmonella>. The contamination rate was 13.2% and serotypes <em>S. brandenburgem>, <em>S. typhimuriumem>, <em>S. agonaem>, <em>S. derbyem> and <em>S. hadorem> were identified.

    KEY-WORDS: <em>Salmonella>; chicken; chicken carcasses; commercial cuts.

    No presente estudo foram analisadas, com vistas ao isolamento de <em>Salmonella>, 53 amostras de carcaças e cortes comerciais de frangos colhidas em supermercados e casas de carnes de Goiânia - GO. Observou-se freqüência média do patógeno de 13,2%, sendo identificados os sorotipos <em>E. brandenburgem>, <em>S. typhimuriumem>, <em>S.> <em>agona>, <em>S. derbyem> e <em>S. Hadarem>.

    PALAVRAS-CHAVE: <em>Salmonella>; frango de corte; carcaça; cortes comerciais.

  10. Chemical Composition and Biological Activities of the Essential Oils from <em>Duguetia lanceolataem> St. Hil. Barks

    Maria A. C. Kaplan

    2012-09-01

    Full Text Available Essential oils of <em>Duguetia lanceolataem> barks, obtained at 2 (T2 and 4 h (T4, were identified by gas chromatography and gas chromatography/mass spectrometry. β-Elemene (12.7 and 14.9%, caryophyllene oxide (12.4 and 10.7% and β-selinene (8.4 and 10.4% were the most abundant components in T2 and T4, respectively. The essential oils inhibited the growth of <em>Staphylococcus aureusem>,> Streptococcus pyogenesem>, <em>Escherichia coliem> and <em>Candida albicansem>. The essential oils were cytotoxic against brine shrimp. The extraction time influenced the chemical composition and biological activities of essential oils obtained from the barks of <em>D. lanceolataem>.

  11. DOE/EM Criticality Safety Needs Assessment

    Westfall, Robert Michael [ORNL; Hopper, Calvin Mitchell [ORNL

    2011-02-01

    The issue of nuclear criticality safety (NCS) in Department of Energy Environmental Management (DOE/EM) fissionable material operations presents challenges because of the large quantities of material present in the facilities and equipment that are committed to storage and/or material conditioning and dispositioning processes. Given the uncertainty associated with the material and conditions for many DOE/EM fissionable material operations, ensuring safety while maintaining operational efficiency requires the application of the most-effective criticality safety practices. In turn, more-efficient implementation of these practices can be achieved if the best NCS technologies are utilized. In 2002, DOE/EM-1 commissioned a survey of criticality safety technical needs at the major EM sites. These needs were documented in the report Analysis of Nuclear Criticality Safety Technology Supporting the Environmental Management Program, issued May 2002. Subsequent to this study, EM safety management personnel made a commitment to applying the best and latest criticality safety technology, as described by the DOE Nuclear Criticality Safety Program (NCSP). Over the past 7 years, this commitment has enabled the transfer of several new technologies to EM operations. In 2008, it was decided to broaden the basis of the EM NCS needs assessment to include not only current needs for technologies but also NCS operational areas with potential for improvements in controls, analysis, and regulations. A series of NCS workshops has been conducted over the past years, and needs have been identified and addressed by EM staff and contractor personnel. These workshops were organized and conducted by the EM Criticality Safety Program Manager with administrative and technical support by staff at Oak Ridge National Laboratory (ORNL). This report records the progress made in identifying the needs, determining the approaches for addressing these needs, and assimilating new NCS technologies into EM

  12. Terpene Glycosides from the Roots of <em>Sanguisorba officinalisem> L. and Their Hemostatic Activities

    Wei Sun; Zi-Long Zhang; Xin Liu; Shuang Zhang; Lu He; Zhe Wang; Guang-Shu Wang

    2012-01-01

    Guided by a hemostasis bioassay, seven terpene glycosides were isolated from the roots of <em>Sanguisorba officinalisem> L. by silica gel column chromatography and preparative HPLC. On the grounds of chemical and spectroscopic methods, their structures were identified as citronellol-1-<em>O>-α-l-arabinofuranosyl-(1→6)-β-d-glucopyranoside (1), geraniol-1-<em>O>-α-l-arabinofuranosyl-(1→6)-β-d-glucopyranoside (

  13. Brasil em obras, peões em luta, sindicatos surpreendidos

    Roberto Véras

    2014-05-01

    Full Text Available O presente artigo aborda as revoltas e greves ocorridas em março de 2011 em canteiros de obras do Programa de Aceleração do Crescimento (PAC, do Governo Federal, que alcançou grande repercussão nacional. Tem como foco os conflitos e as negociações, envolvendo trabalhadores, sindicatos, empresários, Governo, Justiça, Ministério Público do Trabalho, entre outros atores, estabelecidos durante a construção das Usinas Hidroelétricas de Jirau e Santo Antônio, situadas no Norte do país. Visou identificar as posições dos referidos atores e, sob uma perspectiva sociológica, propor reflexões sobre as potencialidades e os limites da atividade sindical. Que questões trouxeram tais acontecimentos ao sindicalismo, quanto às relações deste, de um lado, com suas próprias bases e, de outro, com o atual projeto governamental?

  14. Burnout em residentes de enfermagem

    Gianfábio Pimentel Franco; Alba Lúcia Bottura Leite de Barros; Luiz Antônio Nogueira-Martins; Sandra Salloum Zeitoun

    2011-01-01

    Enfermeiros Residentes podem experimentar desgaste físico e emocional decorrentes do cotidiano da Residência de Enfermagem. O objetivo deste estudo foi determinar a incidência do Burnout em Residentes de Enfermagem. Realizou-se um estudo exploratório, descritivo, analítico e longitudinal-prospectivo com 16 Residentes, no período de dois anos. Utilizou-se o Maslach Burnout Inventory traduzido e validado para o Brasil e um instrumento de dados sociodemográficos/ocupacionais. Dos Residentes, 17....

  15. Il ruolo dell'Arvicola delle nevi <em>Chionomys nivalisem> come specie-preda: un'analisi della situazione italiana

    Armando Nappi

    2003-10-01

    Full Text Available L?Arvicola delle nevi <em>Chionomys nivalisem>, a causa della sua particolare nicchia ecologica costituita, nella sua componente spaziale, dai suoli pietrosi nei cui interstizi vive, viene di norma considerata una preda poco rappresentata nelle catene trofiche. In Italia, eccettuato un dato incerto di cattura da <em>Buteo buteoem>, è risultata predata da cinque specie di serpenti (<em>Coronella austriacaem>, <em>Elaphe longissimaem>, <em>Vipera aspisem>, <em>V. berusem>, <em>V. ursiniiem>, sei di uccelli (<em>Aquila chrysaetosem>, <em>Strix alucoem>, <em>Bubo buboem>, <em>Aegolius funereusem>, <em>Asio otusem> e cinque di mammiferi (<em>Vulpes vulpesem>, <em>Mustela nivalisem>, <em>M. ermineaem>, <em>Martes> sp., <em>Felis catusem>. In alcuni casi, come in <em>Vipera berusem> a Passo Fedaia (BL, <em>Asio otusem> a S. Valentino alla Muta (BZ, <em>Mustela ermineaem> nel Parco Naturale Adamello-Brenta (TN con percentuali piuttosto consistenti, rispettivamente del 69.6%, 60.71% e 35.89%. In uno studio su <em>Vulpes vulpesem> nel Parco Nazionale Gran Paradiso (TO-AO è risultata essere la preda dominante tra i roditori. Sempre nella stessa area, è interessante la predazione da <em>Aegolius funereusem> per la quale risulta, dopo <em>Clethrionomys glareolusem>, la preda più rappresentata (27.97% malgrado, da uno studio di trappolamento di micromammiferi effettuato intorno all?area di nidificazione, risulti assente rivelando così un comportamento esplorativo del rapace diretto proprio alla ricerca dell?arvicola delle nevi. Da una prima analisi sembrerebbe dunque da rivalutare il ruolo di questo roditore come preda, anche considerando il contributo in biomassa che può rappresentare. Su un totale di 184 dati reperiti sulla presenza della specie in Italia, 28 (15.2% derivano da residui di predazione. Benché non risulti un quantitativo alto comparato ad altri micromammiferi è comunque significativo

  16. Bioassay-Guided Antidiabetic Study of <em>Phaleria macrocarpaem> Fruit Extract

    Mohd Z. Asmawi

    2012-04-01

    Full Text Available An earlier anti-hyperglycemic study with serial crude extracts of <em>Phaleria macrocarpaem> (PM fruit indicated methanol extract (ME as the most effective. In the present investigation, the methanol extract was further fractionated to obtain chloroform (CF, ethyl acetate (EAF, <em>n>-butanol (NBF and aqueous (AF fractions, which were tested for antidiabetic activity. The NBF reduced blood glucose (<em>p> < 0.05 15 min after administration, in an intraperitoneal glucose tolerance test (IPGTT similar to metformin. Moreover, it lowered blood glucose in diabetic rats by 66.67% (<em>p> em>< 0.05, similar to metformin (51.11%, glibenclamide (66.67% and insulin (71.43% after a 12-day treatment, hence considered to be the most active fraction. Further fractionation of NBF yielded sub-fractions I (SFI and II (SFII, and only SFI lowered blood glucose (<em>p em>< 0.05, in IPGTT similar to glibenclamide. The ME, NBF, and SFI correspondingly lowered plasma insulin (<em>p em>< 0.05 and dose-dependently inhibited glucose transport across isolated rat jejunum implying an extra-pancreatic mechanism. Phytochemical screening showed the presence of flavonoids, terpenes and tannins, in ME, NBF and SFI, and LC-MS analyses revealed 9.52%, 33.30% and 22.50% mangiferin respectively. PM fruit possesses anti-hyperglycemic effect, exerted probably through extra-pancreatic action. Magniferin, contained therein may be responsible for this reported activity.

  17. Dois funcionalistas franceses em confronto

    Rafael Eugenio Hoyos-Andrade

    2001-02-01

    Full Text Available

    Comparamos, neste artigo, de modo aproximativo e provisório, dois modelos de análise sintática e dois métodos de representação gráfica dessas análises: trata-se dos modelos e métodos de André Martinet e Lucien Tesnière. Depois de algumas considerações introdutórias relativas ao funcionalismo dos autores em causa, arrolamos um conjunto de características que aproximam ou opõem os seus respectivos modelos. Apresentamos, em seguida, no intuito de beneficiar os estudantes universitários de sintaxe, as representações gráficas de um mesmo enunciado segundo o método estemático de Tesnière e segundo o método de visualização das relações sintáticas de Martinet, para concluir que os dois modelos/métodos, embora diferentes, poderiam eventualmente ser integrados num único sistema de análise e representação.

  18. O MAL EM ERIC WEIL

    Daniel Benevides Soares

    2014-12-01

    Full Text Available O mal ocupa a condição de problema para a filosofia desde a Antiguidade. Passando pelas ponderações dos estóicos e de Santo Agostinho, tratado no contexto da teodiceia por Leibniz, o mal aporta como objeto de preocupação para a moral em Kant. Muito conhecido por sua definição como um kantiano pós-hegeliano, é com Kant, mas indo além dele que Weil trata do mal, pensando-o como formas de violência. Considerada como o outro do sentido e da razão, a violência se manifesta de maneiras variadas. Nosso intuito é determinar o conceito de mal em Eric Weil enquanto forma de violência. Para isso, nos propomos a situar no esquema conceitual da violência duas formas de mal discutidas por Weil nos seus textos: o mal radical e o mal diabólico.

  19. Ser marrano em Minas Colonial

    Anita Novinsky

    2001-01-01

    Full Text Available Baseado em 57 Processos inéditos de marranos (cristãos-novos, conversos presos em Minas Gerais no século XVIII, este artigo procura mostrar seu papel na Idade de Ouro do Brasil. 64% deles eram mercadores e 23% eram mineiros. Pertenciam à classe média e raros eram os magnatas. Nenhum dele esteve envolvido no tráfico negreiro. Acusados do crime de judaísmo e de pertencerem a sociedades secretas, representaram 42% dos brasileiros condenados à morte. Ser marrano entre os portugueses no Brasil mais um sentimento e uma visão de mundo do que uma prática religiosa.Based on 57 unknown trials of marranos (new christians, conversos arrested by the Inquisition in Minas Gerais in the XVIII the century, this article shows their role in shapping the history of Minas Gerais. 64% of them were merchants na 23% were miners. Most of them belonged to a middle class and rare were those whit great fortunes. No one of them were engaged in slave trade. Acused of judaism and of participating in secret society the marranos of Minas represented 42% of the Brazilian burned at the stake. Being a marrano in Minas Gerais was not always being a crypto-new. Marranism in Brasil was a feeling and a approach to life.

  20. Terapia breve em hospital fechado

    Paulo Cesar Sandler

    1978-09-01

    Full Text Available O presente estudo cuida de pacientes "difíceis" da clínica psiquiátrica e psicoterápica: os pacientes que apresentam o fenômeno da atuação, cuja aproximação terapêutica tem se demonstrado problemática, mesmo com o advento das modernas técnicas de psicoterapia e das comunidades terapêuticas. Os diagnósticos psiquiátricos destes pacientes variam: são neuróticos, apresentam transtornos de pesonalidade, são psicóticos e quase sempre com graves doenças secundárias: alcoolismo e toxicomania (Códigos Classificação Internacional de Doenças 295 a 299, 300. 301, 303 e 304. Propõe-se, à luz da compreensão psicanalítica destas pessoas, um tratamento psicoterápico breve, focal, com o auxílio de uma hospitalização em regime fechado. Para a avaliação dos resultados do método psicoterápico diádico, criaram-se critérios de cura coerentes com a proposta terapêutica, e equiparam-se estes critérios clínicos a valores numéricos. Utilizando 36 pacientes internados, verificou-se até que ponto eles alcançavam estes critérios. Cada paciente recebeu um escore - valor numérico - que expressou o seu desempenho durante o tratamento em regime de internação, e sua conduta pós-alta hospitalar, durante um seguimento médio de 21 meses. Estes 36 pacientes puderam ser divididos em dois grupos: os de evolução favorável (obtivemos 36,1% e os de evolução desfavorável. Por meio de uma amostra sistemática composta por 41 pacientes que não foram submetidos ao método, internados no mesmo período de tempo, no mesmo hospital, e pareados com nossa casuística, fêz-se uma comparação estatística entre os percentis obtidos nas evoluções dos dois grupos. O valor do teste estatístico, o chi quadrado, não foi significante. O exame das proporções correspondentes a cada patologia em separado sugeriu fortemente que o tratamento estaria indicado nas neuroses e nas psicoses, mas que seria realmente ineficaz nos transtornos de

  1. Alimentazione di <em>Marmota marmotaem> in praterie altimontane delle dolomiti bellunesi

    Alessandro Rudatis

    2006-03-01

    Full Text Available Abstract The diet of <em>Marmota marmotaem> in the mountain prairie of south-eastern Italian Alps. Diet composition of two family groups of alpine marmots was investigated in two areas of the Agordino’s Dolomites (Italian Alps in June-September 2001, by means of microscopic analysis of faeces and of direct observation of feeding activity. During the whole period of activity, a high consume of Angiosperms was confirmed, especially plants in flower; among them the “graminoids” seemed to play an important role only during the initial part of the active period. Generally vegetative parts predominated over flowers. The ingestion of animal preys was not confirmed by the analysis of droppings. Comparing diet composition of the two groups, Graminaceae (<em>Poa>, <em>Phleum>, Compositae (<em>Achillea>, Cyperaceae/Juncaceae, Leguminosae (<em>Anthyllis>, Rosaceae, and Labiatae (<em>Prunella>, <em>Stachys> formed the bulk of marmot diet in the study areas. Diet showed low diversity considering the abundance of plant species in the surrounding environment. Food resources were probably used in relation to their easy digestibility, with a high content in proteins, sugar and water. The knowledge of vegetation features in relation to marmot trophic habits can represent a useful tool for the management of this species. Riassunto Il regime alimentare di due gruppi di Marmotta alpina è stato studiato in giugno-settembre 2001 in due aree delle Dolomiti agordine (SE Italia, attraverso l’analisi microscopica delle feci e l’osservazione diretta dell’attività alimentare. Durante tutto il periodo di attività si è notato un forte consumo di Angiosperme, specialmente piante a fiore, mentre le ”graminoidi” sembra giochino un ruolo importante all’inizio della stagione. In generale le parti vegetali predominano sui fiori. L’ingestione di prede animali non è stata

  2. Feeding tests on captive otters <em>Lutra lutraem>

    Elena Ferrario

    1995-12-01

    Full Text Available Abstract The study was carried out during 1990-91 in "La Torbiera" Faunistic Park (Piemonte region, northern Italy. Feeding experiments were conducted on 4 pairs of otters (<em>Lutra lutraem> of 2-7 year old, housed in enclosures of 210-360 m² in size with ponds of 20-33 m² and about 1 m in depth. Three foraging tests were done on a pair using eels <em>Anguilla anguillaem>, rainbow trout <em>Salmo gairdneriem> and roach <em>Rutilus erythrophthalmusem>, in order to collect data on dive duration and foraging success. Both parameters varied widely and were influenced by the behaviour of fish prey. For eels, slow moving fishes, the dive duration and the foraging success were lower than those of other fish species. Feeding correction factors, calculated as ratio between the weight of ingested fish and the dry weight of remains in spraints, were determined from a total of 21 experiments carried out on eels, rudd <em>Scardinius erythrophthalmusem> and bleak <em>Alburnus alburnus alborellaem>. The factors differed widely between the fish species and the highest value was found for eels. Riassunto Test alimentari sulla lontra <em>Lutra lutraem> in cattività - Lo studio è stato effettuato nel 1990-91 presso il Parco Faunistico "La Torbiera" di Agrate Conturbia (Novara, Piemonte. I test alimentari sono stati condotti su 4 coppie di lontra (<em>Lutra lutraem> di età compresa tra 2 e 7 anni, tenute in recinti di 210-360 m² provvisti di vasche di 20-33 m² profonde 1 m circa. Tre test sono stati effettuati su una coppia somministrando quantità note di Anguilla <em>Anguilla anguillaem>, Trota iridea <em>Salmo gairdneriem> e Triotto <em>Rutilus erythrophthalmusem>, al fine di valutare la durata delle immersioni e il successo di predazione. Entrambi i parametri variavano ampiamente tra le specie ittiche considerate ed erano influenzati dal comportamento delle stesse. Per l

  3. Elongation Factor 1β' Gene from <em>Spodoptera exiguaem>: Characterization and Function Identification through RNA Interference

    Li-Na Zhao

    2012-06-01

    Full Text Available Elongation factor (EF is a key regulation factor for translation in many organisms, including plants, bacteria, fungi, animals and insects. To investigate the nature and function of elongation factor 1β' from <em>Spodoptera> exiguaem> (SeEF-1β', its cDNA was cloned. This contained an open reading frame of 672 nucleotides encoding a protein of 223 amino acids with a predicted molecular weight of 24.04 kDa and pI of 4.53. Northern blotting revealed that <em>SeEF-1β'em> mRNA is expressed in brain, epidermis, fat body, midgut, Malpighian tubules, ovary and tracheae. RT-PCR revealed that <em>SeEF-1β'em> mRNA is expressed at different levels in fat body and whole body during different developmental stages. In RNAi experiments, the survival rate of insects injected with<em> SeEF-1β'em>> em>dsRNA was 58.7% at 36 h after injection, which was significantly lower than three control groups. Other elongation factors and transcription factors were also influenced when EF-1β' was suppressed. The results demonstrate that <em>SeEF-1β' em>is a key gene in transcription in <em>S. exiguaem>.

  4. Echolocation calls and morphology in the Mehelyi’s (<em>Rhinolophus mehelyiem> and mediterranean (<em>R. euryaleem> horseshoe bats: implications for resource partitioning

    Egoitz Salsamendi

    2006-03-01

    Full Text Available Abstract <em>Rhinolophus euryaleem> and <em>R. mehelyiem> are morphologically very similar species and their distributions overlap extensively in the Mediterranean basin. We modelled their foraging behaviour using echolocation calls and wing morphology and, assuming niche segregation occurs between the two species, we explored how it is shaped by these factors. Resting frequency of echolocation calls was recorded and weight, forearm length, wing loading, aspect ratio and wing tip shape index were measured. <em>R. mehelyiem> showed a significantly higher resting frequency than <em>R. euryaleem>, but differences are deemed insufficient for dietary niche segregation. Weight and forearm length were significantly larger in <em>R. mehelyiem>. The higher values of aspect ratio and wing loading and a lower value of wing tip shape index in <em>R. melehyiem> restrict its flight manoeuvrability and agility. Therefore, the flight ability of <em>R. mehelyiem> may decrease as habitat complexity increases. Thus, the principal mechanism for resource partitioning seems to be based on differing habitat use arising from differences in wing morphology. Riassunto Ecolocalizzazione e morfologia nei rinolofi di Mehely (<em>Rhinolophus mehelyiem> e euriale (<em>R. euryaleem>: implicazioni nella segregazione delle risorse trofiche. <em>Rhinolophus euryaleem> e <em>R. mehelyiem> sono specie morfologicamente molto simili, la cui distribuzione risulta largamente coincidente in area mediterranea. Il comportamento di foraggiamento delle due specie è stato analizzato in funzione delle caratteristiche dei segnali di ecolocalizzazione e della morfologia alare, ed è stata valutata l’incidenza di questi fattori nell’ipotesi di una segregazione delle nicchie. È stata rilevata la frequenza a riposo dei segnali ultrasonori, così come il peso, la lunghezza dell’avambraccio, il carico alare, e due

  5. Inhibition of <em>Burkholderia multivoransem> Adhesion to Lung Epithelial Cells by Bivalent Lactosides

    Trinidad Velasco-Torrijos

    2012-08-01

    Full Text Available <em>Burkholderia cepaciaem> complex (Bcc is an opportunistic pathogen in cystic fibrosis patients which is inherently resistant to antimicrobial agents. The mechanisms of attachment and pathogenesis of Bcc, a group of 17 species, are poorly understood. The most commonly identified Bcc species in newly colonised patients,<em> Burkholderia multivoransem>, continues to be acquired from the environment. Development of therapies which can prevent or reduce the risk of colonization on exposure to Bcc in the environment would be a better alternative to antimicrobial agents. Previously, it has been shown that Bcc strains bound to many glycolipid receptors on lung epithelia. Using a real-time PCR method to quantify the levels of binding of <em>B. multivoransem> to the lung epithelial cells, we have examined glycoconjugate derivatives for their potential to inhibit host cell attachment. Bivalent lactosides previously shown to inhibit galectin binding significantly reduced the attachment of <em>B. multivoransem> to CF lung epithelial cells at micromolar concentrations. This was in contrast to monosaccharides and lactose, which were only effective in the millimolar range. Development of glycoconjugate therapies such as these, which inhibit attachment to lung epithelial cells, represent an alternative means of preventing infection with inherently antimicrobially resistant pathogens such as <em>B. multivoransem>.

  6. Antioxidative Properties of Crude Polysaccharides from <em>Inonotus obliquusem>

    Jinyou Duan

    2012-07-01

    Full Text Available The mushroom <em>Inonotus obliquusem>> em>has been widely used as a folk medicine in Russia, Poland and most of the Baltic countries. In this study, water-soluble and alkali-soluble crude polysaccharides (IOW and IOA were isolated from <em>I. obliquusem>, and the carbohydrate-rich fractions IOW-1 and IOA-1 were obtained respectively after deproteination and depigmentation. Their contents, such as neutral carbohydrate, uronic acid and protein, were measured. Their antioxidant properties against chemicals-induced reactive species (ROS including 1,1'-Diphenyl-2-picrylhydrazyl (DPPH radical, hydroxyl radical and superoxide anion radical, as well as their protective effects on H2O2-induced PC12 cell death were investigated. Results showed that <em>I. obliquusem> polysaccharides can scavenge all ROS tested above in a dose-dependent manner. IOA and its product IOA-1 could rescue PC12 cell viability from 38.6% to 79.8% and 83.0% at a concentration of 20µg/mL. Similarly, IOW and its product IOW-1 at the same dose, can also increase cell viability to 84.9% and 88.6% respectively. The antioxidative activities of water-soluble and alkali-soluble polysaccharide constituents from <em>I. obliquusem> might contribute to diverse medicinal and nutritional values of this mushroom.

  7. Synthesis and Antibacterial Evaluation of New <em>N>-acylhydrazone Derivatives from Dehydroabietic Acid

    Wen Gu

    2012-04-01

    Full Text Available A series of new <em>N>-acylhydrazone derivatives were synthesized in good yields through the reactions of dehydroabietic acid hydrazide with a variety of substituted arylaldehydes. The structures of the synthesized compounds were confirmed by IR, 1H- and 13C-NMR, ESI-MS, elemental analysis and single crystal X-ray diffraction. From the crystal structure of compound 4l, the C=N double bonds of these <em>N>-acylhydrazones showed (<em>E>-configuration, while the NMR data of compounds 4aq indicated the existence of two rotamers for each compound in solution. The target compounds were evaluated for their antibacterial activities against four microbial strains. The result suggested that several compounds exhibited pronounced antibacterial activities. Particularly, compound 4p exhibited good antibacterial activity against <em>Staphylococcus aureusem> and <em>Bacillus subtilisem> comparable to positive control. The possible antibacterial metabolism and the strategy for further optimization of this compound were also discussed.

  8. Training for Defense? From Stochastic Traits to Synchrony in Giant Honey Bees (<em>Apis dorsataem>

    Gerald Kastberger

    2012-08-01

    Full Text Available In Giant Honey Bees, abdomen flipping happens in a variety of contexts. It can be either synchronous or cascaded, such as in the collective defense traits of shimmering and rearing-up, or it can happen as single-agent behavior. Abdomen flipping is also involved in flickering behavior, which occurs regularly under quiescent colony state displaying singular or collective traits, with stochastic, and (semi- synchronized properties. It presumably acts via visual, mechanoceptive, and pheromonal pathways and its goals are still unknown. This study questions whether flickering is preliminary to shimmering which is subject of the <em>fs em>(flickering-shimmering-transition> hypothesis? We tested the respective prediction that trigger sites (<em>ts> at the nest surface (where shimmering waves had been generated show higher flickering activity than the alternative non-trigger sites (<em>nts>. We measured the flickering activity of <em>ts>- and <em>nts>-surface bees from two experimental nests, before and after the colony had been aroused by a dummy wasp. Arousal increased rate and intensity of the flickering activity of both <em>ts>- and <em>nts> cohorts (P < 0.05, whereby the flickering intensity of <em>ts>-bees were higher than that of <em>nts>-bees (P < 0.05. Under arousal, the colonies also increased the number of flickering-active <em>ts>- and <em>nts>-cohorts (P < 0.05. This provides evidence that cohorts which are specialist at launching shimmering waves are found across the quiescent nest zone. It also proves that arousal may reinforce the responsiveness of quiescent curtain bees for participating in shimmering, practically by recruiting additional trigger site bees for expanding repetition of rate and intensity of shimmering waves. This finding confirms the <em>fs-transition> hypothesis and constitutes evidence that flickering is part of a basal colony-intrinsic information system

  9. NOVOS PARASITÓIDES EM OVOS DE Dirphia Araucariae (LEPIDOPTERA, ATTACIDAE NEW PARASITOIDS IN EGGS OF Dirphia araucariae (LEP.; ATTACIDAE

    Jácomo Divino Borges

    2007-09-01

    Full Text Available

    Foram observados novos parasitóides nos ovos de <em>Dirphia> <em>araucariae> Jones, 1908 (<em>Lepidoptera>, <em>Attacidae>. A porcentagem do parasitismo em ovos do hospedeiro, coletados na Fazenda Marabá, Município de Congonhinhas (PR foi de 20,71%, e as espécies de parasitóides identificadas e suas freqüências do: <em>Anastatus> <em>sp> (0,45%; <em>A. charitos em>De Santis, 1982 (1,08%; A. (<em>Proanastatus> <em>excavatus> (De Santis, 1952 (1,97%; <em>Ooencyrtus> <em>sp> (17,86%; <em>O.> <em>blastothricoide> De Santis, 1988 (43,65%; e <em>O. dirphiaeem> De Santis, 1988 (34,99%.

    PALAVRAS-CHAVE: <em>Anastatus> <em>spp>; <em>Araucaria> <em>angustifolia>; controle biológico; lagarta-da-araucária; <em>Ooencyrtus sppem>; pinheiro-do-Paraná.

    The parasitism in Dirphia araucariae Jones, 1908 (<em>Lepidoptera>, <em>Attacidae> was studied and the parasitoids observed on its eggs collected in 'Fazenda Marabá’, Municipality of Congonhinhas, State of Paraná, Brazil, were the following: <em>Anastatus> <em>sp>.; <em>A>. <em>charitos> De Santis, 1982; <em>A.> (<em>Proanastatus> <em>excavatus> (De Santis, 1952; <em>Ooencyrtus> <em>sp.>; <em>O. blastothricoideem> De Santis, 1988, and <em>O. dirphiaeem> De Santis, 1988, with a frequency of 0.45%, 1.08%, 1.97%, 17.86%, 43.65% and 34.99%, respectively.

    KEY-WORDS: Biological control; Paraná-pine.

  10. Efeito dos fungicidas Fosetil-Al e Metalaxil na colonização de mudas de <em>Citrus sinensisem> (L.) Osbeck enxertadas em <em>C. limonem> (L.) Burmf Effect of the fungicides Fosetyl-Al and Metalaxyl on arbuscular mycorrhizal colonization of seedlings of <em>Citrus sinensisem> (L.) Osbeck grafted onto <em>C. limonem> (L.) Burmf

    Luiz Antônio Graciolli; Vera Lúcia Ramos Bononin; Rosilaine Carrenho

    2000-01-01

    O efeito dos fungicidas Fosetil-Al e Metalaxil, recomendados para prevenção e controle da gomose, sobre o desenvolvimento de fungos micorrízicos arbusculares (FMA) em Citrus sinensis Osbeck enxertado em C. limon Burmf foi investigado. Três experimentos foram realizados em 1990 e 1992, em Casa Branca e Ilha Solteira, estado de São Paulo, Brasil, respectivamente. Coletas de raízes foram feitas após a aplicação dos fungicidas, em intervalos mensais e semanais em Casa Branca, e a cada três dias e...

  11. Uso de dexmedetomidina em anestesiologia

    Villela Nivaldo Ribeiro

    2003-01-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: A dexmedetomidina é um novo agonista alfa2-adrenérgico que apresenta relação de seletividade entre os receptores alfa2:alfa1 de 1600:1, com importante ação sedativa e analgésica, bom controle hemodinâmico frente ao estresse e que pode produzir, por si só, anestesia. Este agente tem sido empregado para promover analgesia e sedação no período pós-operatório e nas unidades de tratamento intensivo. Devido à suas propriedades, recentemente, a dexmedetomidina passou a ser utilizada também na sala de operações, como fármaco coadjuvante em anestesia. Assim, este artigo faz uma revisão da literatura com relação ao uso da dexmedetomidina na prática anestésica. CONTEÚDO: São apresentados os principais trabalhos com o emprego da dexmedetomidina em Anestesiologia, seja como medicação pré-anestésica, ou durante o ato anestésico-cirúrgico. O mecanismo de ação dos fármacos alfa2-agonistas e as propriedades farmacocinéticas e farmacodinâmicas da dexmedetomidina também são revistos neste artigo. CONCLUSÕES: O uso da dexmedetomidina como medicação pré-anestésica, durante anestesia, ou no período pós-operatório, promove boa estabilidade hemodinâmica. Há redução do consumo de anestésicos durante a anestesia. Os pacientes sedados com a dexmedetomidina podem ser despertados, quando solicitados, e tornarem-se cooperativos. Mesmo doses elevadas do fármaco não provocam depressão respiratória. Bradicardia é um efeito adverso observado com freqüência, problema amenizado pela administração lenta da droga. Assim, a dexmedetomidina torna-se importante recurso adicional para a prática clínica da Anestesiologia, com possibilidade de uso em diversos tipos de pacientes e procedimentos cirúrgicos.

  12. HAICAIS EM MANOEL DE BARROS

    Profª Drª Nery Reiner

    2012-09-01

    Full Text Available O objetivo deste artigo, voltado para a criação poética de Manoel de Barros, é focalizar o conceito da obra de arte como jogo. Escolhemos Manoel de Barros, porque o lúdico, a brincadeira e o humor estão sempre presentes em sua obra. A escolha dos dados, o encaixe de palavras escolhidas a dedo, no eixo paradigmático, formam um caleidoscópio, mostrando novas estruturas, deixando explícito o desejo de brincar com os elementos linguísticos e da natureza. Para este trabalho, escolhemos, para análise, pequenos poemas semelhantes aos haicais japoneses e outros menores ainda, com dois ou até um verso apenas. Contaremos, nesta pesquisa, com a ajuda de teóricos como Johan Huizinga, Ezra Pound, Octavio Paz, Lévi-Strauss, Olga Savary e Alfredo Bosi.

  13. Project X RFQ EM Design

    Romanov, Gennady; /Fermilab; Hoff, Matthew; Li, Derun; Staples, John; Virostek, Steve; /LBNL

    2012-05-09

    Project X is a proposed multi-MW proton facility at Fermi National Accelerator Laboratory (FNAL). The Project X front-end would consist of an H- ion source, a low-energy beam transport (LEBT), a CW 162.5 MHz radio-frequency quadrupole (RFQ) accelerator, and a medium-energy beam transport (MEBT). Lawrence Berkeley National Laboratory (LBNL) and FNAL collaboration is currently developing the designs for various components in the Project X front end. This paper reports the detailed EM design of the CW 162.5 MHz RFQ that provides bunching of the 1-10 mA H- beam with acceleration from 30 keV to 2.1 MeV.

  14. Poderes locais em perspectiva comparada

    Fernando Ruivo

    2012-10-01

    Full Text Available “Os romances não começam como queremos, mas como eles querem”, afirmou Gabriel Garcia Marquez. Similarmente, as nossas vidas nunca decorrem como individualmente as tínhamos pensado, desejado e planeado. Ora, tal como as nossas vidas, também as reformas político-administrativas e as políticas públicas territorializadas não começam, deve dizer-se, como queremos, isto é, como pensámos, desejámos e planeámos, mas em boa parte como elas querem, como se tivessem, afinal, alguma vida própria, a qual...

  15. Osteoporose em caprinos Osteoporosis in goats

    Fábio B. Rosa; Glauco J.N. Galiza; Ricardo B. Lucena; Taiara M. Silva; Rafaela A. Caprioli; Claudio S.L. Barros; Rafael A. Fighera; Glaucia D Kommers

    2013-01-01

    Foi realizado um estudo de casos de osteoporose em caprinos provenientes de uma propriedade na área de abrangência do LPV-UFSM, determinando a epidemiologia, o quadro clínico-patológico e discutindo os prováveis mecanismos patogenéticos. Cinco cabras, fêmeas, SRD, de seis meses a seis anos de idade foram afetadas. As cabras eram mantidas em campo nativo, sem suplementação com ração e sob superlotação. Os principais sinais clínicos foram emagrecimento, dificuldade de locomoção e permanência em...

  16. O Trabalho Plurativo em Presidente Prudente

    Sandro Mauro Guirro

    2011-11-01

    Full Text Available Atualmente, vêm ocorrendo mudanças no campo brasileiro, principalmente nas  propriedades localizadas próximas a malha urbana da cidade. Esse processo se verifica com mais intensidade em cidades como Campinas, Sorocaba, Jundiaí entre outras cidades próximas aos grandes centros urbanos. Em Presidente Prudente, esse processo dá sinais de existência em resposta à crise pela qual vem passando, sobretudo a pequena agricultura. Está-se diante da intensificação das mudanças de funções do trabalho inserido na lavra agrária.

  17. Determinantes do endividamento municipal em Portugal

    Ferreira, Fernando Diogo Vilaça

    2011-01-01

    Dissertação de mestrado em Economia, Mercados e Políticas Públicas O objectivo desta dissertação consiste em analisar os factores que explicam o índice de endividamento dos Municípios portugueses entre 2003 e 2007. Em Portugal, os municípios surgiram no pós-25 de Abril de 1974 com atribuições desempenhadas até aí pela Administração Central. No entanto, nem sempre foram acompanhadas por transferências suficientes provocando o recurso ao crédito. Este endividamento municipal t...

  18. Sistemas de Indicadores de Desempenho em Projetos

    Julia Garaldi Borges; Marly Monteiro de Carvalho

    2011-01-01

    O presente trabalho visa propor um sistema de indicadores de desempenho de projetos, a partir da análise da importância dos critérios de sucesso em projetos. A abordagem metodológica foi a de pesquisa-ação desenvolvida em uma empresa de engenharia, com atuação em diversos setores. Foram utilizadas várias fontes de evidência, tais como análise do atual sistema de indicadores da empresa, análise dos projetos nas bases de dados e entrevistas com representantes do escritório de projetos e com ger...

  19. Tricolemoma em coelho Tricholemmoma in a rabbit

    Krishna Duro de Oliveira; Ticiana do Nascimento França; Alexander Pérez González; Paulo Vargas Peixoto

    1999-01-01

    Tricolemoma é um raro tumor benigno, composto por células epiteliais claras derivadas da bainha externa da raiz do folículo piloso. Esses tumores têm sido descritos no homem e raramente em cães. No presente relato, descreve-se a ocorrência de um tricolemoma, de 1cm de diâmetro em um coelho adulto, cujas características histológicas são muito semelhantes às descritas para esses tumores em cães.Tricholemmoma is a rare benign tumor composed of clear epithelial cells derived from the outer root s...

  20. Comorbidades psiquiátricas em dependentes químicos em abstinência em ambiente protegido Psychiatric comorbidities in abstinent drug addict in a protected environment

    Adriana Raquel Binsfeld Hess; Rosa Maria Martins de Almeida; André Luiz Moraes

    2012-01-01

    O objetivo desta pesquisa foi verificar a frequência de comorbidades psiquiátricas, utilizando Mini International Neuropsychiatric Interview, em diferentes grupos de dependentes químicos em abstinência, em ambiente protegido, classificados de acordo com o tipo de droga utilizada: (1) grupo controle (n = 37); (2) dependentes em abstinência de álcool (n = 8); (3) dependentes em abstinência de álcool, maconha e crack/cocaína (n = 24); e (4) dependentes em abstinência de múltiplas substâncias psi...

  1. 3-(1<em>H>-Indol-3-yl-4-(morpholin-4-ylcyclobut-3-ene-1,2-dione

    Johann Grünefeld

    2012-06-01

    Full Text Available 3-(1<em>H>-Indol-3-yl-4-(morpholin-4-ylcyclobut-3-ene-1,2-dione was obtained in good yields (72–82% by nucleophilic substitution of 3-chloro-4-(1<em>H>-indol-3-ylcyclobut-3-ene-1,2-dione with morpholine.

  2. Three dimensional geometric morphometric study of the Ethiopian <em>Myomys - Stenocephalemysem> complex (Murinae, Rodentia

    Carlo Fadda

    2000-06-01

    Full Text Available Abstract Geometric morphometrics was used to investigate the Ethiopian <em>Myomys - Stenocephalemysem> complex, and to suggest possible explanations for differences in size and shape. The four species of the complex (<em>M. albipesem>, <em>M. ruppiem>, <em>S. griseicaudaem>, <em>S. albocaudataem> and a Kenyan species, <em>M. fumatusem>, were studied using Procrustes analysis of three dimensional landmarks collected over the skull. All these species occur in very different habitats, from forests at 1000 m up to the Afro Alpine moorlands above 4000 m. There is a substantial contradiction between phylogenetic relationships based on chromosomal rearrangements and allozymes (two distinct lineages corresponding to the two genera, and mtDNA (<em>Stenocephalemys> being paraphyletic. Geometric morphometrics supports the former hypothesis. Partial Least-Squares analysis shows a significant relation between variation in size and shape and altitude, which strongly suggests that adaptation is a major causal factor for divergence in the morphology of the skull. Size increases with altitude, paralleling a clinal change in shape, which involves stenocephaly as characterising the highland species. This shape modification allows the rodents to scan the sky efficiently for birds, which represent the main category of predators in the Afro Alpine moorlands.

  3. Identification of Volatile Components of Liverwort (<em>Porella cordaeanaem> Extracts Using GC/MS-SPME and Their Antimicrobial Activity

    Maria Elisabetta Guerzoni

    2012-06-01

    Full Text Available Chemical constituents of liverwort (<em>Porella cordaeanaem> extracts have been identified using solid-phase microextraction-gas chromatography mass spectrometry (SPME-GC/MS. The methanol, ethanol and ethyl acetate extracts were rich in terpenoids such as sesquiterpene hydrocarbons (53.12%, 51.68%, 23.16%, and monoterpene hydrocarbons (22.83%, 18.90%, 23.36%, respectively. The dominant compounds in the extracts were β-phellandrene (15.54%, 13.66%, 12.10% and β-caryophyllene (10.72%, 8.29%, 7.79%, respectively. The antimicrobial activity of the extracts was evaluated against eleven food microorganisms using the microdilution and disc diffusion methods. The minimum inhibitory concentration (MIC varied from 0.50 to 2.00 mg/mL for yeast strains (<em>Saccharomyces cerevisiae 635em>, <em>Zygosacharomyces bailii 45em>, <em>Aerobasidium pullulans L6Fem>, <em>Pichia membranaefaciens OC 71em>, <em>Pichia membranaefaciens OC 70em>, <em>Pichia anomalaem> <em>CBS 5759em>, <em>Pichia anomala DBVPG 3003em> and<em> em>>Yarrowia lipolytica RO13em>, and from 1.00 to 3.00 mg/mL for bacterial strains<em> em>(Salmonella> <em>enteritidis 155em>, <em>Escherichia coli 555em> and <em>Listeria monocytogenes 56Lyem>. Methanol extract showed better activity in comparison with ethanol and ethyl acetate extracts. High percentages of monoterpene and sesquiterpene hydrocarbons could be responsible for the better antimicrobial activity.

  4. Feeding habits of the genet <em>Genetta genettaem> in an Iberian continental wetland

    Maite Sánchez

    2009-02-01

    Full Text Available Abstract This study quantified the diet of the genet <em>Genetta genettaem> in an Iberian continental wetland, the Galachos Nature Reserve, northern Spain, based on the frequency with which items appeared in five monthly-surveyed latrines during 2004-2005. Prey types were identified <em>de visuem> using identification keys and expert advice. the genet was confirmed as an opportunistic and generalist predator, its diet including mammals (95.0%, plants (68.3% and arthropods (60.0% as main prey. With the exception of a newly available prey species, the alien crayfish <em>Procambarus clarkiiem>, the genet probably consumed arthropods because of chance encounters rather than active search. The consumption of fruits and small mammals varied seasonally. Riassunto Alimentazione della genetta <em>Genetta genettaem> in un'area umida continentale della penisola iberica La dieta della genetta è stata investigata in un'area umida interna della Spagna settentrionale, la riserva Naturale dei Galachos. La frequenza di ciascuna categoria alimentare è stata espressa come numero di occorrenze mansili in cinque latrine monitorate per il periodo 2004-2005. Le categorie sono state identificate <em>de visuem> tramite chiavi per il riconoscimento o l'intervento di singoli esperti. I risultati confermano il comportamento alimentare generalista e opportunista della genetta. La dieta ha incluso principalmente mammiferi (95%, vegetali (68.3% e artropodi (60%. Questi ultimi, con l'eccezione del gambero <em>Procambarus clarkiiem>, introdotto e di recente presenza, sono probabilmente utilizzati in modo opportunistico, piuttosto che in seguito a ricerca attiva. L'uso dei frutti e dei micromammiferi varia stagionalmente.

  5. Chemical Composition and Biological Activities of the Essential Oils from <em>Duguetia lanceolataem> St. Hil. Barks

    Kaplan, Maria A. C.; Rodarte, Mírian P.; Maria P. H. Amaral; Araújo, Aílson A. L.; Míriam A. O. Pinto; Maria S. Alves; Glauciemar Del-Vechio-Vieira; Orlando V. Sousa

    2012-01-01

    Essential oils of <em>Duguetia lanceolataem> barks, obtained at 2 (T2) and 4 h (T4), were identified by gas chromatography and gas chromatography/mass spectrometry. β-Elemene (12.7 and 14.9%), caryophyllene oxide (12.4 and 10.7%) and β-selinene (8.4 and 10.4%) were the most abundant components in T2 and T4, respectively. The essential oils inhibited the growth of <em>Staphylococcus aureusem>,> Streptococcus pyogenesem>, <em>Escherichia coli&l...

  6. Influência do ambiente em cultivares de feijoeiro-comum em cerrado com baixa altitude

    Helton Santos Pereira; Válter Martins de Almeida; Leonardo Cunha Melo; Adriane Wendland; Luís Cláudio de Faria; Maria José Del Peloso; Mariana Cruzick de Souza Magaldi

    2012-01-01

    O objetivo desse trabalho foi identificar genótipos de feijoeiro-comum com alta adaptabilidade e estabilidade de produção e verificar a ocorrência de interação genótipos x ambientes (GxA) para diferentes caracteres em algumas regiões de cerrado com baixa altitude no Estado do Mato Grosso. Foram realizados 12 ensaios em blocos casualizados, com três repetições, em épocas de semeadura da seca e do inverno, no Estado do Mato Grosso, em 2008 e 2009. Os ensaios foram compostos por 19 genótipos com...

  7. Trophic Interactions between Generalist Predators and the Two Spotted Spide Mite, Tetranychus urticae in, Strawberry

    Jacobsen, Stine Kramer

    The two spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is a major cause of pest damage worldwide. Its host range includes among many the strawberry crop, a high value crop in Denmark as well as in many other temperate and subtropical regions. Chemical control of T. urticae...... occurrence and diversity of predatory insects and predatory mites in Danish strawberry fields and surrounding vegetation is lacking, as is the knowledge of the potential of generalist insect predators to control T. urticae. The overall objective of this PhD thesis was to investigate the trophic interactions...... between natural enemies, in particular generalist predators and the two spotted spider mite, T. urticae, in strawberry. This was done by investigating interactions of T. urticae and its natural enemies as influenced by cropping practice and the surrounding vegetation (Manuscript I) as well as more...

  8. Jasmonic acid induced resistance in grapevines to a root and leaf feeder.

    Omer, A D; Thaler, J S; Granett, J; Karban, R

    2000-06-01

    We investigated the effects of induced resistance to the folivore Pacific spider mite, Tetranychus pacificus McGregor (Acari: Tetranychidae), as well as the root-feeding grape phylloxera Daktulosphaira vitifoliae (Fitch) (Homoptera: Phylloxeridae) in grapevines using exogenous applications of the natural plant inducer, jasmonic acid. Foliar jasmonic acid application at concentrations that caused no phytotoxicity significantly reduced the performance of both herbivores. There were less than half as many eggs produced by spider mites feeding on the induced leaves compared with control grapevine leaves. Induction reduced the numbers of phylloxera eggs and nymphal instars by approximately threefold and twofold, respectively, on induced compared with control grapevine roots. The negative demographic effects of jasmonic acid application appeared to be caused by changes in fecundity for the Pacific spider mite, and possibly changes in development rate and fecundity for grape phylloxera. PMID:10902339

  9. Characterization of the Antibacterial Activity and the Chemical Components of the Volatile Oil of the Leaves of <em>Rubus parvifoliusem> L.

    Yongqing Cai

    2012-06-01

    Full Text Available <em>Rubus parvifoliusem> L. (Rp is a medicinal herb that possesses antibacterial activity. In this study, we extracted the volatile oil from the leaves of Rp to assess its antibacterial activity and analyze its chemical composition. A uniform distribution design was used to optimize the extraction procedure, which yielded 0.36% (<em>w/w> of light yellowish oil from the water extract of Rp leaves. We found that the extracted oil effectively inhibited the growth of a wide range of Gram positive and negative bacteria, including <em>Staphylococcus aureusem>, <em>Staphylococcus epidermidisem>, <em>Enterococcus faecalisem>, <em>Escherichia coliem>, <em>Pseudomonas aeruginosaem>, <em>Acinetobacter baumaniiem>, <em>Bacillus cloacaeem>, and <em>Klebsiella pneumoniaeem>. We further analyzed the components contained in the hydro-distillated Rp volatile oil by gas chromatography-mass spectroscopy. Twenty nine compounds were identified, including 4-hydroxy-3-methoxystyrene (66%, 3,7,11,15-tetramethyl-2-hexadecen-1-ol (10% and 4-<em>tert>-butylbenzoic acid (2%. Our results suggest that one or multiple constituents contained in Rp volatile oil may account for its antibacterial activity.

  10. EMS in Taiwan: past, present, and future.

    Chiang, Wen-Chu; Ko, Patrick Chow-In; Wang, Hui-Chih; Yang, Chi-Wei; Shih, Fuh-Yuan; Hsiung, Kuang-Hua; Ma, Matthew Huei-Ming

    2009-01-01

    Taiwan is a small island country located in East Asia. From around 1995 modern concepts of the EMS were imported and supported by legislation. Considerable progress has since been made towards the construction of an effective pre-hospital care system. This article introduces the current status of the EMS in Taiwan, including the systems, response configurations, funding, personnel, medical directorship, and outcome research. The features and problems of in-hospital emergency care are also discussed. Key areas for further development in the country vary depending on regional differences in available resource and population density. An analysis of the strength, weakness, opportunity, and threats of the evolving EMS in Taiwan could be an example for other countries where the EMS is undergoing a similar process of development and optimisation. PMID:19059690

  11. Platelet-Activating Factor (PAF Antagonistic Activity of a New Biflavonoid from <em>Garcinia nervosaem> var. <em>pubescens> King

    Azura Abdul Ghani

    2012-09-01

    Full Text Available The methanol extract of the leaves of <em>Garcinia nervosaem> var. <em>pubescens> King, which showed strong inhibitory effects on platelet-activating factor (PAF receptor binding, was subjected to bioassay-guided isolation to obtain a new biflavonoid, II-3,I-5, II-5,II-7,I-4',II-4'-hexahydroxy-(I-3,II-8-flavonylflavanonol together with two known flavonoids, 6-methyl-4'-methoxyflavone and acacetin. The structures of the compounds were elucidated by spectroscopic methods. The compounds were evaluated for their ability to inhibit PAF receptor binding to rabbit platelets using 3H-PAF as a ligand. The biflavonoid and acacetin showed strong inhibition with IC50 values of 28.0 and 20.4 µM, respectively. The results suggest that these compounds could be responsible for the strong PAF antagonistic activity of the plant.

  12. Probióticos em avicultura

    Gil de los Santos João Rodrigo

    2005-01-01

    Full Text Available Durante os últimos anos, o incremento de toxinfecções alimentares em humanos pela ingestão de produtos avícolas contaminados com bactérias, sugeriu que produtos oriundos de aves portadoras dessas bactérias podem veiculá-las ao consumidor, provocando doenças entéricas em humanos. Essas observações e a restrição, por parte do mercado consumidor, ao uso de antimicrobianos na produção animal, aumentaram o interesse mundial pelos probióticos e, conseqüentemente, o número de pesquisas realizadas. Esta revisão mostra os avanços e a situação em que se encontram os probióticos em relação à indústria avícola.

  13. Biocerâmicas em Endodontia

    Oliveira, Pedro Miguel da Silva

    2014-01-01

    Introdução e Objectivos: Biocerâmicas incluem materiais cerâmicos projetados especificamente para uso em Medicina e Medicina Dentária. O objetivo deste trabalho é efetuar uma revisão bibliográfica em que demonstre a avaliação das propriedades físico-químicas das biocerâmicas e o seu uso em Endodontia. Radiopacidade, Fluidez, pH e Libertação de íões cálcio foram analisados e comparados com outros materiais usados em Endodontia. Material e Métodos: Para a realização da pesq...

  14. <em>Helicobacter pyloriem> Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation

    Hsueh-Fen Juan

    2012-08-01

    Full Text Available <em>Helicobacter pyloriem> (<em>H. pyloriem>, the human stomach pathogen, lives on the inner surface of the stomach and causes chronic gastritis, peptic ulcer, and gastric cancer. Plasma membrane repair response is a matter of life and death for human cells against physical and biological damage. We here test the hypothesis that <em>H. pyloriem> also causes plasma membrane disruption injury, and that not only a membrane repair response but also a cell proliferation response are thereby activated. Vacuolating cytotoxin A (VacA and cytotoxin-associated gene A (CagA have been considered to be major <em>H. pyloriem> virulence factors. Gastric cancer cells were infected with <em>H. pyloriem> wild type (vacA+/cagA+, single mutant (ΔvacA or ΔcagA or double mutant (ΔvacA/ΔcagA strains and plasma membrane disruption events and consequent activation of membrane repair components monitored. <em>H. pyloriem> disrupts the host cell plasma membrane, allowing localized dye and extracellular Ca2+ influx. Ca2+-triggered members of the annexin family, A1 and A4, translocate, in response to injury, to the plasma membrane, and cell surface expression of an exocytotic maker of repair, LAMP-2, increases. Additional forms of plasma membrane disruption, unrelated to <em>H.> pylori em>exposure, also promote host cell proliferation. We propose that <em>H. pyloriem> activation of a plasma membrane repair is pro-proliferative. This study might therefore provide new insight into potential mechanisms of <em>H. pyloriem>-induced gastric carcinogenesis.

  15. <em>Hedyotis diffusaem> Willd Inhibits Colorectal Cancer Growth <em>in Vivoem> via Inhibition of STAT3 Signaling Pathway

    Jun Peng; Wei Xu; Aling Shen; Zhenfeng Hong; Jianwei Zeng; Youzhi Zhan; Ling Zhang; Lili Wang; Lihui Wei; Jiumao Lin; Qiaoyan Cai

    2012-01-01

    Signal Transducer and Activator of Transcription 3 (STAT3), a common oncogenic mediator, is constitutively activated in many types of human cancers; therefore it is a major focus in the development of novel anti-cancer agents. <em>Hedyotis diffusaem> Willd has been used as a major component in several Chinese medicine formulas for the clinical treatment of colorectal cancer (CRC). However, the precise mechanism of its anti-tumor activity remains largely unclear. Using a CRC mous...

  16. Local feeding specialization of the red fox (<em>Vulpes vulpesem>) in response to eastern cottontail (<em>Sylvilagus floridanusem>) introduction (NW Italy)

    Alessandro Balestrieri; Luigi Remonti; Claudio Prigioni

    2006-01-01

    Abstract To appreciate the influence of the introduction of the Eastern cottontail (<em>Sylvilagus floridanusem>) on the food habits of the red fox (Vulpes vulpes), between June 1998 and February 2000 fox diet was investigated by means of scat analysis (N=115) in a 250 ha wide Natural Reserve of NW Italy, and compared with data collected in the same area prior to cottontail colonization (1988-1989). Comparison included also the diet of badgers (...

  17. Net Energy, CO2 Emission and Land-Based Cost-Benefit Analyses of <em>Jatropha> em>Biodiesel: A Case Study of the Panzhihua Region of Sichuan Province in China

    Xiangzheng Deng

    2012-06-01

    Full Text Available Bioenergy is currently regarded as a renewable energy source with a high growth potential. Forest-based biodiesel, with the significant advantage of not competing with grain production on cultivated land, has been considered as a promising substitute for diesel fuel by many countries, including China. Consequently, extracting biodiesel from <em>Jatropha> curcasem> has become a growing industry. However, many key issues related to the development of this industry are still not fully resolved and the prospects for this industry are complicated. The aim of this paper is to evaluate the net energy, CO2 emission, and cost efficiency of <em>Jatropha> biodiesel as a substitute fuel in China to help resolve some of the key issues by studying data from this region of China that is well suited to growing <em>Jatropha>. Our results show that: (1 <em>Jatropha> biodiesel is preferable for global warming mitigation over diesel fuel in terms of the carbon sink during <em>Jatropha> tree growth. (2 The net energy yield of <em>Jatropha> biodiesel is much lower than that of fossil fuel, induced by the high energy consumption during <em>Jatropha> plantation establishment and the conversion from seed oil to diesel fuel step. Therefore, the energy efficiencies of the production of <em>Jatropha> and its conversion to biodiesel need to be improved. (3 Due to current low profit and high risk in the study area, farmers have little incentive to continue or increase <em>Jatropha> production. (4 It is necessary to provide more subsidies and preferential policies for <em>Jatropha> plantations if this industry is to grow. It is also necessary for local government to set realistic objectives and make rational plans to choose proper sites for <em>Jatropha> biodiesel development and the work reported here should assist that effort. Future research focused on breading high-yield varieties, development of efficient field

  18. A Favela posta em Cena

    Fabiana Valdoski Ribeiro

    2010-08-01

    Full Text Available Em uma realidade de urbanização calcada no aprofundamento dos processos de expropriação e espoliações se consolida o fenômeno da produção de uma morfologia intitulada Favela, que continua a representar um desafio teórico e prático para pesquisadores. Este desafio é enfrentado de várias formas sendo uma delas desenhada no âmbito de duas publicações do Observatório das Favelas (Rio de Janeiro assentadas na busca conceitual do termo favela e objetivando a efetivação de políticas públicas. Esta meta remonta a trajetória deste Observatório que criado em 2001 pensa a inserção da Favela no âmbito das políticas de Estado. A primeira publicação intitulada “Favela: alegria e dor na cidade” (2005 se compõem de quatro capítulos que expõem um movimento da história de formação das favelas na cidade do Rio de Janeiro, as formas de intervenção do Estado, a prática cotidiana dos moradores como manifestação das transgressões diante as opressões e finaliza com as propostas de caminhos possíveis de superação das desigualdades e ampliação da democracia. O argumento central dos autores se direciona para o questionamento das visões de homogeneidade, ausência, carência e apartamento das favelas diante da cidade apontando a urgência de construção de um projeto de cidade democrático e fraterno.Ao longo do texto os autores vão descrevendo os exemplos destas visões e analisa o resultado delas por meio de como a população residente vive, pois são ora invisíveis, ora criminalizados, ora caracterizados enquanto um problema social estabelecendo, portanto, uma lógica autoritária que legitimará no decorrer da história das favelas, as intervenções estatais. Este movimento molda uma linha argumentativa que tenta explicitar o negativo diante a produção possível para o “morar’, que calcado na impossibilidade do acesso à terra pelo imperativo da propriedade privada empurra grandes contingentes populacionais

  19. EMS Stations, EMS: Represents the locations of EMS Stations in Washington County., Published in 2009, 1:1200 (1in=100ft) scale, Washington County GIS Office.

    NSGIC GIS Inventory (aka Ramona) — This EMS Stations dataset, published at 1:1200 (1in=100ft) scale, was produced all or in part from Orthoimagery information as of 2009. It is described as 'EMS:...

  20. Development of 101 Gene-based Single Nucleotide Polymorphism Markers in Sea Cucumber, <em>Apostichopus japonicusem>>

    Wei Lu

    2012-06-01

    Full Text Available Single nucleotide polymorphisms (SNPs are currently the marker of choice in a variety of genetic studies. Using the high resolution melting (HRM genotyping approach, 101 gene-based SNP markers were developed for <em>Apostichopus japonicusem>, a sea cucumber species with economic significance for the aquaculture industry in East Asian countries. HRM analysis revealed that all the loci showed polymorphisms when evaluated using 40 <em>A. japonicusem> individuals collected from a natural population. The minor allele frequency ranged from 0.035 to 0.489. The observed and expected heterozygosities ranged from 0.050 to 0.833 and 0.073 to 0.907, respectively. Thirteen loci were found to depart significantly from Hardy–Weinberg equilibrium (HWE after Bonferroni corrections. Significant linkage disequilibrium (LD was detected in one pair of markers. These SNP markers are expected to be useful for future quantitative trait loci (QTL analysis, and to facilitate marker-assisted selection (MAS in <em>A. japonicusem>.

  1. LESÕES FOLIARES EM MUDAS DE PEQUIZEIRO (Caryocar brasiliensis Cambess) LEAF LESIONS OF YOUNG PLANTS OF PEQUIZEIRO

    Yvo de Carvalho; Leila Garcez de Araújo

    2007-01-01

    Foram descritas as características de lesões e morfologia de colônias de <em>Pestalotia> <em>sp>., <em>Phomopsis spem>., <em>Stigmella spem>., <em>Chaetophoma sp.em>, <em>Helminthosporium sp.em> e <em>Epicoccum sp. em>obtidas de folhas de mudas de pequizeiro em viveiros nos municípios de Goiânia e Hidrolândia (GO). ...

  2. Acaricidal properties of a Chenopodium-based botanical.

    Chiasson, H; Bostanian, N J; Vincent, C

    2004-08-01

    The emulsifiable concentrate UDA-245 [25% EC (vol:vol)], based on an essential oil extract from Chenopodium ambrosioides variety ambrosioides, a North American herbaceous plant, was compared with commercially available pesticides for their effectiveness to control the adult stage and egg hatch of the twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae) and the European red mite, Panonychus ulmi (Koch) (Acari: Tetranychidae). After a laboratory bioassay with adult twospotted spider mites, a 0.5% concentration of UDA-245 was more effective than 0.7% (AI) of neem oil (Neem Rose Defense). After a similar bioassay with the European red mite, a 0.5% concentration UDA-245 was as effective as 0.006% (AI) of abamectin (Avid). UDA-245 at 0.5% significantly reduced egg hatch of the twospotted spider mite, 5 and 9 d after treatment and of the European red mite 6 d after treatment. Egg hatch was significantly lower using 0.006% (AI) of abamectin, 0.7% of neem oil, and 1.0% insecticidal soap than UDA-245. Residual tests indicated that UDA-245 may be persistent in the environment only for a few hours. Only 23% mortality was noted when mites were introduced on bean leaves 1 h after treatment with a 2% concentration of UDA-245. At the recommended dose of 0.5%, UDA-245 was not considered phytotoxic for most plants tested, i.e., lettuce, roses, and tomatoes. Results suggest that a greenhouse integrated pest management program using UDA-245 could effectively and selectively control mite infestations by treating "hot spots" with negligible effect on biological control agents when treating before introduction or when natural enemies are absent. PMID:15384350

  3. A MORTE EM CINCO ATOS

    André Lira

    2015-01-01

    Full Text Available ResumoOs avanços técnicos atuais põem adiante muitasexpectativas relativas à nossa mortalidade.Devedores de uma longa tradição de dicotomias,como corpo e alma, cultura e naturezae muitas outras, nós estamos realmente observandoa virtualização crescente da realidade.Por que isso está ocorrendo e a que isso estáconduzindo? Nós iremos propor uma dançaapaixonada com a questão da morte, não parasuperá-la ou repeli-la, como normalmente éexperienciada no mundo moderno. Em vezdisso, nessa mesma dança, tentaremos redescobrirnossas raízes terrenas, articulando odivino e a singularidade de nossas vidas comodevidos ao horizonte que a morte revela.Morte como horizonte, isto é, a própria possibilidadede ser, quer dizer que a mortalidadeé o próprio dom da felicidade, o permanentee essencial sentido da história. E o horizontedas possibilidades que a morte oferece a cadaum dos seres humanos aparece como um silênciosagrado, sempre um espelho escuro requisitandoe questionando nossas identidadese certezas, nos pondo em movimento. Nessesentido, recuperar o sentido existencial damorte é pedir abrigo, mais uma vez, no ventremisterioso da terra.Palavras-chave: Morte; corpo; terra.AbstractTh e current technical advancements putforward many expectations concerning ourmortality. In debt of a long tradition of dichotomies,such as body and soul, cultureand nature and many others, we are indeedwatching the increasing virtualization of reality.Why is that happening and what is it leadingto? We will propose a passionate dancewith the question of death, not to overcomeor repel it, as it normally is experienced in themodern world. Instead, in that very dance,we will try to rediscover our earthly roots, articulatingthe divinity and singularity of ourlives as due to the horizon that death unveils.Death as horizon, that is, the possibility ofbeing itself, means that mortality is the truegift of happiness, the everlasting and essentialmeaning of history

  4. Avaliação de parâmetros clínicos e hematológicos de eqüinos submetidos a um programa de controle estratégico de Amblyomma cajennense (Fabricius, 1787 (Acari: Ixodidae Evaluation of clinical and hematological parameters of equines submitted to a strategic control program of Amblyomma cajennense (Fabricius, 1787 (Acari: Ixodidae

    A.P. Cunha

    2008-02-01

    Full Text Available Estudaram-se aspectos clínicos e hematológicos em eqüinos submetidos a um programa de controle estratégico de A. cajennense. Os tratamentos carrapaticidas foram realizados a cada sete dias e divididos em dois módulos, o primeiro com início em abril de 2004, e o segundo com início em julho do mesmo ano, utilizando-se a base química piretróide - cipermetrina na concentração de 0,015%. Além do acompanhamento clínico dos animais, foram realizados hemogramas completos antes e após o programa. As dosagens bioquímicas de bilirrubinas, gama-glutamiltransferase (GGT, aspartato aminotransferase (AST, creatina kinase (CK, proteína total, albumina e globulinas, foram realizadas antes, durante e ao final do programa. Os resultados demonstraram que houve uma melhora no quadro hematológico dos animais após o programa de controle. Os tratamentos carrapaticidas, na forma em foram aplicados, não provocaram alterações desfavoráveis nos parâmetros clínicos e hematológicos dos eqüinos. Tais informações podem ser consideradas na busca de alternativas viáveis e seguras para o controle dessa espécie de carrapato.Clinical and hematological parameters were studied in equines submitted to a strategic control program of Amblyomma cajennense. The acaricide treatments were carried to each seven days and divided in two batteries, the first one began in April 2004 and the second in July 2004. A pyrethroid chemical base - 0.015% cypermethrin was used. Clinical examinations of the animals and complete hemograms were carried before and after the control program of the tick. Seric dosages of bilirrubins, gamma-glutamiltransferase (GGT, aspartate aminotransferase (AST, creatin kinase (CK, total protein, albumin, and globulins, were carried before and throughout the experiment. The results showed an improvement in the hematological parameters of the animals after the end of control program. The acaricide treatments did not cause undesirable alterations

  5. Eficiência de extratos de Agave sisalana (Perrine) sobre o ácaro rajado Tetranychus urticae (Koch) e ocorrência de fitotoxidez em plantas de algodoeiro (Gossypium hirsutum L. r latifolium Hutch).

    Barrêto, Artur Franco; Araújo, Egberto; Bonifácio, Benedito Ferreira

    2010-01-01

    Objetivou-se avaliar a eficiência dos extratos frescos (24 horas após a maceração) e curtidos (35 dias depois) de dois genótipos de Agave (Agave sisalana Perrine) no controle do ácaro rajado Tetranychus urticae (Koch) (Tetranychidae) do algodoeiro (Gossypium hirsutum L. r latifolium Hutch) e os efeitos de fitotoxidez dessas aplicações. Os tratamentos foram: plantas não tratadas; extrato fresco de Agave sisalana; extrato fresco do híbrido 11648; extrato curtido de A. sisalana; e, extrato curti...

  6. Commensal <em>Pseudomonas> Species Isolated from Wastewater and Freshwater Milieus in the Eastern Cape Province, South Africa, as Reservoir of Antibiotic Resistant Determinants

    Anthony I. Okoh

    2012-07-01

    Full Text Available <em>Pseudomonas> species are opportunistic pathogens with implications in a wide range of diseases including cystic fibrosis and sickle cell anaemia. Because of their status as multidrug resistant (MDR and extremely drug resistant (XDR bacteria <em>Pseudomonas> species represent a threat to public health. Prevalence, antibiogram and associated antibiotic resistant genes of <em>Pseudomonas> species isolated from freshwater and mixed liquor environments in the Eastern Cape Province of South Africa were assessed. Polymerase chain reaction (PCR based technique was used to identify the isolates and screen for antibiotic resistant genes. The result shows occurrence of <em>Pseudomonas> spp. in freshwater and mixed liquor as follows: 71.42% and 37.5% (<em>P. putidaem>, 14.28% and 31.25% (<em>P. flourescensem>, 7.14% and 6.25% (<em>P. aeruginosaem> and 7.14% and 25% for other <em>Pseudomonas> species respectively. Disk diffusion antibiogram of the <em>Pseudomonas> isolates from the two locations showed 100% resistance to penicillin, oxacillin, clindamycin, rifampicin and 100% susceptibility to ciprofloxacin and gentamicin with varied percentage resistances to cephalothin, nalidixic acid, tetracycline, and ampicillin. The <em>blaTEM> antibiotic resistant gene was detected in 12.5% of <em>P. putidaem>, 57.14% of <em>P. fluorescensem>, 100% <em>P. aeruginosaem> and 40% in other <em>Pseudomonas> species. Similarly, Integrons conserved segment were detected in 12.5% of <em>P. putidaem>, 57.14% of <em>P. fluorescensem>, 100% of <em>P. aeruginosaem> and 40% of other <em>Pseudomonas> species. The presence of <em>blaTEM> gene and integrons conserved segment in some of the isolates is worrisome and suggest <em>Pseudomona>s species as important reservoirs of multidrug resistance genes in the Eastern Cape Province environment.

  7. Preussianone, a New Flavanone-Chromone Biflavonoid from <em>Garcinia preussiiem> Engl.

    Gerhard Bringmann; Kurt Hostettmann; Jean-Luc Wolfender; Alain Meli Lannang; Nkengfack, Augustin E; Barbara Hertlein-Amslinger; Karine Ndjoko-Ioset; Bernadette Biloa Messi

    2012-01-01

    A new flavanone-chromone biflavonoid, preussianone (1), has been isolated from the leaves of <em>Garcinia preussiiem>, along with four known biflavonoids. The absolute stereostructures were elucidated by chemical, spectroscopic, and chiroptical methods. The biological properties of the new biflavonoid against several bacterial strains were evaluated.

  8. <em>In Vitro em>Phytotoxicity and Antioxidant Activity of Selected Flavonoids

    Rita Patrizia Aquino

    2012-05-01

    Full Text Available The knowledge of flavonoids involved in plant-plant interactions and their mechanisms of action are poor and, moreover, the structural characteristics required for these biological activities are scarcely known. The objective of this work was to study the possible <em>in vitro em>phytotoxic effects of 27 flavonoids on the germination and early radical growth of <em>Raphanus sativus em>L.> and <em>Lepidium sativumem> L., with the aim to evaluate the possible structure/activity relationship. Moreover, the antioxidant activity of the same compounds was also evaluated. Generally, in response to various tested flavonoids, germination was only slightly affected, whereas significant differences were observed in the activity of the various tested flavonoids against radical elongation. DPPH test confirms the antioxidant activity of luteolin, quercetin, catechol, morin, and catechin. The biological activity recorded is discussed in relation to the structure of compounds and their capability to interact with cell structures and physiology. No correlation was found between phytotoxic and antioxidant activities.

  9. Enzymatic Properties and Mutational Studies of Chalcone Synthase from <em>Physcomitrella patensem>

    Mahiran Basri

    2012-08-01

    Full Text Available <em>Pp>CHS is a member of the type III polyketide synthase family and catalyses the synthesis of the flavonoid precursor naringenin chalcone from <em>p>-coumaroyl-CoA. Recent research reports the production of pyrone derivatives using either hexanoyl-CoA or butyryl-CoA as starter molecule. The Cys-His-Asn catalytic triad found in other plant chalcone synthase predicted polypeptides is conserved in <em>Pp>CHS. Site directed mutagenesis involving these amino acids residing in the active-site cavity revealed that the cavity volume of the active-site plays a significant role in the selection of starter molecules as well as product formation. Substitutions of Cys 170 with Arg and Ser amino acids decreased the ability of the <em>Pp>CHS to utilize hexanoyl-CoA as a starter molecule, which directly effected the production of pyrone derivatives (products. These substitutions are believed to have a restricted number of elongations of the growing polypeptide chain due to the smaller cavity volume of the mutant’s active site.

  10. Antibacterial Secondary Metabolites from the Cave Sponge <em>Xestospongia em>sp.

    Sridevi Ankisetty

    2012-05-01

    Full Text Available Chemical investigation of the cave sponge <em>Xestospongia em>sp. resulted in the isolation of three new polyacetylenic long chain compounds along with two known metabolites. The structures of the new metabolites were established by NMR and MS analyses. The antibacterial activity of the new metabolites was also evaluated.

  11. Actinomicose da Mama em Gestante

    Mottola Jr Juvenal

    2001-01-01

    Full Text Available A actinomicose mamária é doença inflamatória rara, com poucos casos descritos na literatura. Pode ser primária da mama quando resultante de traumas na pele e papila mamária, e, secundária, quando de origem toracopleural. Sua apresentação clínica é variável, devendo ser diferenciada das doenças mais comuns, dentre elas as mastites e também as neoplasias, como o carcinoma inflamatório. Seu diagnóstico é realizado pela cultura da secreção, com a identificação das colônias de Actinomyces sp. Seu tratamento é a drenagem, quando indicada, a antibioticoterapia endovenosa e manutenção oral por tempo prolongado. Os autores relatam caso de abscesso retromamário por Actinomyces sp. em gestante de 12 semanas que apresentava tumoração mamária expansiva na mama esquerda.

  12. Dermatite multifatorial em um canino

    C.B. Matos

    2012-12-01

    Full Text Available Relatou-se um caso clínico de dermatite multifatorial em cão, envolvendo Demodex canis, Sporothrix schenckii e bactérias dos gêneros Staphylococcus e Streptococcus. O animal, macho, da raça Pit Bull, com um ano e três meses de idade, apresentou lesões úmidas, hiperêmicas, descamativas e ulcerativas nas regiões do pescoço e do tórax. Exames laboratoriais confirmaram o diagnóstico de infecção concomitante por fungo, bactéria e parasito. A terapia antimicrobiana com moxidectina, itraconazol e amoxicilina foi efetiva, com remissão dos sinais clínicos após 30 dias de tratamento. Este relato salienta a importância do diagnóstico de certeza, que foi determinante para estabelecimento da terapêutica adequada e recuperação do animal.

  13. Monorquidismo em um equino marchador

    Jéssica Fontana de Magalhães

    2015-06-01

    Full Text Available O monorquidismo é um quadro raramente descrito em equinos, que não apresenta descrições prévias na literatura nacional. Trata-se de um equino adulto brasileiro, com oito anos de idade, sem raça definida, porém com características de raças de origem nacional. Por abordagem inguinal do lado esquerdo, localizou-se a túnica vaginal intacta, que, aberta, continha a cauda de um epidídimo mal formado, o ducto deferente e um delgado pedículo vascular, mas sem indícios de testículo. Exames histológicos e hormonais confirmaram a ausência de tecido testicular. Conclui-se que os exames histológicos e hormonais são essenciais para o diagnóstico de monorquidismo que, apesar de raro, também pode acometer equinos nacionais

  14. Self: um conceito em desenvolvimento

    Lídia Suzana Rocha de Macedo

    2012-08-01

    Full Text Available Diversas definições de self coexistem nas teorias e práticas psicológicas. Essa variedade é resultante de bases epistemológicas a partir das quais se adotam estratégias diferentes para abordar e demarcar os limites do objeto em questão e descrevê-lo. Este estudo teve como objetivo oferecer uma revisão dos conceitos de self e uma reflexão sobre como esse conceito se articula nas diferentes abordagens teóricas da psicologia. Destaca-se que dilemas centrais à psicologia do desenvolvimento atravessam o conceito de self. Por essa razão, apresenta-se a tese de que, ao analisar cada definição de self, deve-se buscar responder como cada teoria colocou-se diante das dicotomias estabilidade versus transformação, específico versus universal e mundo interno versus mundo externo.

  15. OECD Guidelines for the Testing of Chemicals, Test No. 226: Predatory mite (<em>Hypoaspis (Geolaelaps) aculeiferem>) reproduction test in soil

    Römbke, L. Becker, B. Dark, Th. Moser, N. Halsall, W. Powley, A. Ruf, C. Scholer, E. Smit, P. Wege, N. Zenz m.fl., J.; Krogh, Paul Henning

    2008-01-01

    replicates for each test concentrations and six to eight control replicates, of 10 animals each, are recommended. At 20 oC, the test lasts 14 days after introducing the females, which usually allows the control offspring to reach the deutonymph stage. The number of surviving females (mortality < 20% for a...... valid test) and the number of juveniles per test vessel (at least 50 for a valid test) are determined. The fecundity of the mites exposed to the test substance is compared to that of controls in order to determine the ECx (e.g. EC10, EC50) or the No Observed Effect Concentration (NOEC). Any observed......This Test Guideline describes a method to assess the effects of chemical substances in soil on the reproductive output of the soil mite species Hypoaspis (Geolaelaps) aculeifer Canestrini (Acari: Laelapidae). It can be used for water soluble or insoluble substances, but not with volatile substances...

  16. Local feeding specialization of the red fox (<em>Vulpes vulpesem> in response to eastern cottontail (<em>Sylvilagus floridanusem> introduction (NW Italy

    Alessandro Balestrieri

    2006-03-01

    Full Text Available Abstract To appreciate the influence of the introduction of the Eastern cottontail (<em>Sylvilagus floridanusem> on the food habits of the red fox (Vulpes vulpes, between June 1998 and February 2000 fox diet was investigated by means of scat analysis (N=115 in a 250 ha wide Natural Reserve of NW Italy, and compared with data collected in the same area prior to cottontail colonization (1988-1989. Comparison included also the diet of badgers (<em>Meles melesem>, considered as potential competitors for food resources. Alien lagomorphs (mean percent volume, Vm% = 68% represented by far the most exploited resource, only three other food items reaching values of mean percent volume barely higher than 5%. Cottontails frequency of occurrence did not vary according either to season or to their reproductive cycle (II-IX vs. X-I, whilst diet niche breadth varied inversely proportional to the use of this key-resource. Overall fox trophic niche breadth varied from 0.64 in 1988-89 to 0.31 in 1998-00 (B, Levin’s index. These findings led us to consider the feeding habits of the fox in the study area as a result of local specialization of a typical generalist carnivore, according to the predictions of optimal foraging theory. No variation occurred in the badger niche breadth since cottontail introduction, whilst niche overlap between foxes and badgers decreased from 0.59 to 0.13 (O, Pianka’s index, possibly reducing competition for food in summer. Riassunto Specializzazione alimentare a livello locale della Volpe <em>Vulpes vulpesem> in risposta all’introduzione del Silvilago <em>Sylvilagus floridanusem> (Italia nord occidentale. Per valutare gli effetti dell’introduzione del Silvilago (<em>Sylvilagus floridanusem> sul comportamento alimentare della volpe (<em>Vulpes vulpesem>, nel periodo giugno 1998-febbraio 2000, la dieta del carnivoro è stata definita tramite l’analisi di 115

  17. Validation report – Results of an International Ring test According to the Draft Guideline: Predatory mite reproduction test in soil (<em>Hypoaspis (Geolaelaps) aculeiferem>) (med bidrag)

    Römbke, Jörg; Becker, B. Dark, Th. Moser, N. Halsall, W. Powley, A. Ruf, C. Scholer, E. Smit, P. Wege, N. Zenz, L.; Krogh, Paul Henning

    A new Test Guideline has been developed, which is designed to be used for assessing the effects of chemical substances in soil on the reproductive output of the soil mite species Hypoaspis (Geolaelaps) aculeifer Canestrini (Acari: Laelapidae). H. aculeifer represents an additional trophic level to...... developed a study protocol which was afterwards used in an international ring test. Twelve laboratories performed in total 48 tests using two test chemicals (dimethoate and boric acid) and two test designs (NOEC, ECx). Only five tests were not valid. Seven tests (plus further work) were intended to clarify...

  18. Infracommunities of intestinal helminths of the Red Fox <em>Vulpes vulpesem> (Linnaeus, 1758 from Italian Alps

    Anna Rita Di Cerbo

    2003-10-01

    Full Text Available <em>Vulpes vulpesem> (Linnaeus, 1758 is one of the most common carnivore in Italy and its spread includes almost the whole national territory. The species shows an high ecological plasticity and a variable diet composition connected in part to human sources. This high adaptability permits the red foxes to colonize different habitats like the suburbs of large cities as well as the small villages located in mountain areas. On the other hand, the tourism pressure seems to assume a great importance in the Alps, also in those areas where the foxes live. So, indirect interactions could take place between these animals and the humans. The role of <em>V. vulpesem> in the zoonoses has not to be understated since this carnivore could transmit parasitic diseases that are able to cause serious pathologies in humans. This study aims just to investigate on intestinal communities of helmiths of <em>V. vulpesem> in order to make progress in current knowledge on epidemiological situation in Italian Alps. During 1998-2003, we have examined 450 foxes from Trentino Alto Adige, Veneto, Lombardia and Valle d'Aosta. The specimens collected were found dead or have been hunted (according to national law n. 157/92 in localities situated between 170 and 2200 m a.s.l. The carcasses were carried to the provincial sections of Zooprofilattici Institutes, where the intestine was drawn by each sample and all the material was sent to the Faculty of Veterinary Medicine of Milan. Parasitological examination of the small intestines was performed by the analysis of the whole sediment and counting technique (SCT. Parasites were preserved in alcohol 70° before to be clarified or stained and identified by microscope (Zeiss Axioscop. Mean abundance, mean intensity and prevalence were calculated for each taxon of helminth. Dates of sampling were grouped within the four seasons. Statistic tests were performed with software package SPSS rel. 11.5 and spatial analysis with the

  19. Teor de nitrato em alface cultivada em sistemas hidropônico e convencional

    Beninni Elisabete R. Yonamini

    2002-01-01

    Full Text Available O monitoramento do teor de nitrato nos alimentos é importante, uma vez que poderão ser formados compostos prejudiciais à saúde humana e animal após sua ingestão. Com o objetivo de avaliar o teor de nitrato presente em alface, foram coletadas e analisadas as folhas de alface crespa comercializada no Município de Londrina, cultivada em sistemas hidropônico e convencional. Os valores encontrados variaram entre 26 e 2.568 mg.kg-1 de peso fresco, sendo que as plantas cultivadas em sistema hidropônico apresentaram teores superiores em relação às cultivadas em sistema convencional, porém abaixo do limite máximo de nitrato permitido para alface, na Europa.

  20. Farmacocinética do propofol em nanoemulsão em cães

    Rafael Messias Luiz; Martielo Ivan Gehrcke; Marcos Paulo Antunes de Lima; Anicleto Poli; Aury Nunes de Moraes; Felipe Hertzing Farias; Nilson Oleskovicz

    2013-01-01

    O propofol em nanoemulsão é uma nova formulação constituída por sistemas nanoemulsionados, caracterizado pela ausência do veículo lipídico. A modificação do veículo pode acarretar alterações farmacocinéticas, resultando em diferentes taxas de distribuição e excreção do propofol. O objetivo deste estudo foi avaliar a farmacocinética de uma nova formulação de propofol em nanoemulsão do tipo óleo em água, comparando com a formulação tradicional em emulsão lipídica. Foram utilizadas seis cadelas ...