abundance acute-phase proteins: Topics by WorldWideScience.org

Molecular cloning, genomic organization, and chromosomal localization of the human pancreatitis-associated protein (PAP) gene

Intrinsically disordered proteins are predicted to be highly abundant and play broad biological roles in eukaryotic cells. In particular, by virtue of their structural malleability and propensity...Full Text Available

2009-01-01

3

Tomato 14-3-3 Protein 7 Positively Regulates Immunity-Associated Programmed Cell Death by Enhancing Protein Abundance and Signaling Ability of MAPKKK ?^[C]^[W]

Pancreatitis-associated protein (PAP) is a secretory pancreatic protein present in small amounts in normal pancreas and overexpressed during the acute phase of pancreatitis. In this paper, the authors describe the cloning, characterization, and chromosomal mapping of the human PAP gene. The gene spans 2748 bp and contains six exons interrupted by five introns. The gene has a typical promoter containing the sequences TATAAA and CCAAT 28 and 52 bp upstream of the cap site, respectively. They found striking similarities in genomic organization as well as in the promoter sequences between the human and rat PAP genes. The human PAP gene was mapped to chromosome 2p12 using rodent-human hybrid cells and in situ chromosomal hybridization. This localization coincides with that of the reg/lithostathine gene, which encodes a pancreatic secretory protein structurally related to PAP, suggesting ...

1994-01-01

4

Increased expression of cytosolic chaperonin CCT in human hepatocellular and colonic carcinoma

Programmed cell death (PCD) is triggered when Pto, a Ser-Thr protein kinase, recognizes either the AvrPto or AvrPtoB effector from Pseudomonas syringae pv tomato. This...Full Text Available

2010-01-01

5

A Mouse Model Expressing a Truncated Form of Ameloblastin Exhibits Dental and Junctional Epithelium Defects

... protein is abundant in cytosolic portions of malignant epithelial tissue (Fig. 3A). In contrast, the degree of CCTβ staining in normal epithelial tissue (Fig. 3B) or connective tissues was much weaker ......

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Assessment of acute phase proteins and oxidative stress status of Nigerians using bleaching agents

SUMMARYAmeloblastin (AMBN) is the second most abundant extracellular matrix protein produced by the epithelial cells called ameloblasts and is found mainly in forming dental enamel....Full Text Available

2009-06-01

7

Transcription of the E2F-1 gene is rendered cell cycle dependent by E2F DNA-binding sites within its promoter.

The disruption of primary innate immune function of the epidermal layer of the skin accounts for the susceptibility of individuals using bleaching agents to localized or systemic infections. This subverted innate immunity in these people may lead to other pathological conditions. The resultant effects of skin bleaching and phagocytes activation in response to infections have not been studied in Nigerians using bleaching agents. The present study therefore assessed the levels of C-reactive protein (CRP), albumin, total antioxidant potential (TAP), total plasma peroxides (TPP), oxidative stress index (OSI) and malonaldehyde (MDA) in the users bleaching agents. Thirty (30) people who had used bleaching agents for average of 4.9 + 1.2 years participated in this study. They were recruited from various schools and markets within the city of Ibadan, Oyo State, Nigeria. Thirty apparently healthy staffs of University College Hospital Ibadan, Ibaadan, Nigeria, who had never ...

8

Detection of behavioral alterations and learning deficits in mice lacking synaptophysin

The cell cycle-regulatory transcription factor E2F-1 is regulated by interactions with proteins such as the retinoblastoma gene product and by cell cycle-dependent alterations in E2F-1 mRNA abundance....Full Text Available

1994-10-01

9

British Library Electronic Table of Contents (United Kingdom)

The integral membrane protein synaptophysin is one of the most abundant polypeptide components of synaptic vesicles. It is not essential for neurotransmission despite its abundance but is believed to modulate the efficiency of the synaptic vesicle cycle. Detailed behavioral analyses were therefore performed on synaptophysin knockout mice to test whether synaptophysin affects higher brain functions. We find that these animals are more exploratory than their wild type counterparts examining novel objects more closely and intensely in an enriched open field arena. We also detect impairments in learning and memory, most notably reduced object novelty recognition and reduced spatial learning. These deficits are unlikely caused by impaired vision, since all electroretinographic parameters measur...

2009-01-01

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Systemic responses to inhaled ozone in mice: cachexia and down-regulation of liver xenobiotic metabolizing genes

Advanced nanoscale separations and mass spectrometry for sensitive high-throughput proteomics

Rats or mice acutely exposed to high concentrations of ozone show an immediate and significant weight loss, even when allowed free access to food and water. The mechanisms underlying this systemic response to ozone have not been previously elucidated. We have applied the technique of global gene expression analysis to the livers of C57BL mice acutely exposed to ozone. Mice lost up to 14% of their original body weight, with a 42% decrease in total food consumption. We previously had found significant up-regulation of genes encoding proliferative enzymes, proteins related to acute phase reactions and cytoskeletal functions, and other biomarkers of a cachexia-like inflammatory state in lungs of mice exposed to ozone. These results are consistent with a general up-regulation of different gene families responsive to NF-#kappa#B in the lungs of the exposed mice. In the present study, we observed significant down-regulation of ...

2005-10-15

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Survey and analysis of simple sequence repeats in the Laccaria bicolor genome, with development of microsatellite markers

We review recent development in separations and mass spectrometric instrumentation for sensitive and high-throughput proteomic analyses. These efforts have been primarily focused on the development of high-efficiency (separation peak capacity of ~103) nanoscale liquid chromatography (nanoLC; e.g., flow rates extending down to ~20 nL/min at optimal separation linear velocities through narrow packed capillaries) in combination with advanced mass spectrometry (MS), including high sensitivity and high resolution Fourier transform ion cyclotron resonance (FTICR) MS. This technology enables MS analysis of low nanogram-level proteomic samples (i.e., nanoscale proteomics) with individual protein identification sensitivity at the low zeptomole-level. The resultant protein measurement dynamic range can reach 106 for nanogram-sized proteomic samples, while more abundant proteins can be detected from complex ...

2005-06-01

12

Cloning, Expression, Crystallization and Preliminary Crystallographic Analysis of a Pentapeptide-repeat Protein (Rfr23) from the Bacterium Cyanothece 51142l

It is becoming clear that simple sequence repeats (SSRs) play a significant role in fungal genome organization, and they are a large source of genetic markers for population genetics and meiotic maps. We identified SSRs in the Laccaria bicolor genome by in silico survey and analyzed their distribution in the different genomic regions. We also compared the abundance and distribution of SSRs in L. bicolor with those of the following fungal genomes: Phanerochaete chrysosporium, Coprinopsis cinerea, Ustilago maydis, Cryptococcus neoformans, Aspergillus nidulans, Magnaporthe grisea, Neurospora crassa and Saccharomyces cerevisiae. Using the MISA computer program, we detected 277,062 SSRs in the L. bicolor genome representing 8% of the assembled genomic sequence. Among the analyzed basidiomycetes, L. bicolor exhibited the highest SSR density although no correlation between relative abundance and the genome sizes was observed. In most genomes the short ...

2011-01-01

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Pleiotropic regulation of macrophage polarization and tumorigenesis by formyl peptide receptor-2

A unique feature of cyanobacteria genomes is the abundance of genes that code for hypothetical proteins containing tandem pentapeptide repeats approximately described by the consensus motif A(N/D)LXX. To date, the structures of two pentapeptide-repeat proteins (PRPs) have been determined, with the tandem pentapeptide-repeat sequences observed to adopt a novel type of right-handed quadrilateral ?-helix, or Rfr-fold, in both structures. One structure, Mycobacterium tuberculosis MfpA, is a 183-residue protein that contains 30 consecutive pentapeptide repeats and appears to offer antibiotic resistance by acting as a DNA mimic. The other structure, Cyanothece 51142 Rfr32, is a 167-residue protein that contains 21 consecutive pentapeptide repeats. The function of Rfr32, like the other 35 hypothetical PRPs identified in the genome of Cyanothece, is unknown. In an effort to understand the ...

2006-01-01

14

British Library Electronic Table of Contents (United Kingdom)

Cancer cells recruit monocytes, macrophages and other inflammatory cells by producing abundant chemoattractants and growth factors, such as macrophage colony-stimulating factor (M-CSF/CSF-1) and monocyte chemoattractant protein-1 (MCP-1/CCL2), to promote tumor growth and dissemination. An understanding of the mechanisms that target cancer cells and regulate tumor microenvironment is essential in designing anticancer therapies. Here, we showed that serum amyloid-A (SAA) and cathelicidin (LL-37) stimulated M-CSF and MCP-1 expression with or without lipopolysaccharide (LPS) administration; conversely, lipoxin-A4 (LXA4) and annexin-A1 (ANXA1) inhibited LPS-induced M-CSF and MCP-1 production by human (HepG2) and mouse (H22) hepatocellular carcinoma cells (HCCs). The effects of LXA4, ANXA1, SAA ...

2011-01-01

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Systems Biology Approach in Chlamydomonas Reveals Connections between Copper Nutrition and Multiple Metabolic Steps[C][W][OA

Functionalized multi-walled carbon nanotubes as affinity ligands

In this work, we query the Chlamydomonas reinhardtii copper regulon at a whole-genome level. Our RNA-Seq data simulation and analysis pipeline validated a 2-fold cutoff and 10 RPKM (reads per kilobase of mappable length per million mapped reads) (~1 mRNA per cell) to reveal 63 CRR1 targets plus another 86 copper-responsive genes. Proteomic and immunoblot analyses captured 25% of the corresponding proteins, whose abundance was also dependent on copper nutrition, validating transcriptional regulation as a major control mechanism for copper signaling in Chlamydomonas. The impact of copper deficiency on the expression of several O2-dependent enzymes included steps in lipid modification pathways. Quantitative lipid profiles indicated increased polyunsaturation of fatty acids on thylakoid membrane digalactosyldiglycerides, indicating a global impact of copper deficiency on the photosynthetic apparatus. Discovery of a putative plastid copper chaperone ...

2011-04-01

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Data Merging for Integrated Microarray and Proteomic Analysis

Functionalization of carbon nanotubes is very challenging for their applications. The paper here describes a new method to functionalize multi-walled carbon nanotubes (MWCNTs) as specific affinity adsorbents. MWCNTs were acid purified and pretreated with (3-aminopropyl)-triethoxysilane (APTES) in order to introduce abundant amino groups on the surface of MWCNTs. After the conversion of amino groups to carboxyl groups by succinic acid anhydride, MWCNTs were attached to protein A or aminodextran using 1-ethyl-3,3' (dimethylamion)-propylcarbodiimide as a biofunctional crosslinker. The incorporation of aminodextran as a spacer arm noticeably increased the binding capacity of the APTES-modified MWCNTs for protein A. The application of affinity MWCNTs for purification of immunoglobulin G was then evaluated. The affinity of MWCNTs with AMD spacer exhibited a high adsorption capacity of {approx}361 {mu}g IgG/mg MWCNT (wet ...

2007-03-21

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Lithium abundance in two halo stars

The functioning of even a simple system is much more complicated than the sum of its genes, proteins and metabolites. A premise of systems biology is that molecular profiling will lead to the discovery and characterization of important disease pathways. However, as multiple levels of effector pathway regulation appear to be the norm rather than the exception, a significant challenge presented by high-throughput genomics and proteomics technologies is to extract the biological implications of complex data. Thus, integration of heterogeneous types of data generated from diverse global technology platforms represents the first challenge in developing the necessary foundational databases needed for predictive modeling of cell and tissue responses. Given the apparent difficulty in defining the correspondence between gene expression and protein abundance measured in several systems to date, how do we make sense of these data and ...

2006-05-10

18

Creation of the iron-group elements in a supernova explosion

Observation of lithium lines in two halo stars could bring some information about /sup 7/Li abundance at the time of the formation of the galaxy.

1981-10-05

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The relative abundances of iron-peak elements produced by the e-process in a supernova outburst are calculated. The results agree quite well with the cosmic abundances of elements in the range Z=23--28.

1980-01-01

20

Biodiversity of Vibrios

Platelet thrombosis in cardiac-valve prostheses

Vibrios are ubiquitous and abundant in the aquatic environment. A high abundance of vibrios is also detected in tissues and/or organs of various marine algae and animals, e.g., abalones, bivalves, corals,...Full Text Available

2004-09-01

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Exploring changes in solar model physics to mitigate the solar abundance problem

The contribution of platelets and clotting factors in thrombosis on cardiovascular prostheses had been quantified with several tracers. Thrombus formation in vivo could be measured semiquantitatively in animal models and patients with indium-111, Technetium-99m labeled platelets, iodine-123, iodine-131 labeled fibrinogen, and In-111 and Tc-99m labeled antibody to the fibrinogen-receptor on the platelet- membrane, or fibrin. The early studies demonstrated that certain platelet-inhibitors, e.g. sulfinpyrazone, aspirin or aspirin- persantine increased platelet survival time with mechanical valves implanted in the baboon model and patients. Thrombus localization by imaging is possible for large thrombus on thrombogenic surface of prosthesis in the acute phase. The majority of thrombus was found in the sewing ring (Dacron) in the acute phase in both the mechanical and tissue valves. The amount of retained ...

1989-06-08

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DEVICE FOR TISSUE CULTURE, PROTEIN EXTRACTION AND PROTEIN CONCENTRATION

No abstract prepared.

2008-01-01

23

J-STORE (Japan)

Full Text Available

2007-12-28

24

Gel-based proteomics of liver cancer progression in rat.

Nonenzymatic glycosylation of human hemoglobin at multiple sites

A significant challenge in proteomics biomarker research is to identify the changes that are of highest diagnostic interest, among the many unspecific aberrations associated with disease burden and inflammation. In the present study liver tissue specimens (n=18) from six experimental stages were collected from the resistant hepatocyte (RH) rat model of liver cancer and analyzed by 2D DIGE. The study included triplicates of regenerating liver, control "sham-operated" liver, three distinct premalignant stages and hepatomas. Out of 81 identified proteins two-thirds were differentially abundant in rat hepatomas compared to control rat liver and, secondly, the majority of proteins were also changed in precursor stages. This underscores the importance of adequate control samples in explorative cancer biomarker research. We confirm several proteomic changes previously identified in human hepatocellular carcinoma (HCC) and we ...

2011-06-06

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Crystal structure of Spot 14, a modulator of fatty acid synthesis

The most abundant minor hemoglobin component of human hemolysate is Hb A1c, which has glucose bound to the N-terminus of the beta chain by a ketoamine linkage. Hb A1c is formed slowly and continuously throughout the 120 day lifespan of the red cell. It can be synthesized in vitro by incubating purified hemoglobin with 14C-glucose. Other minor components, Hb A1a1 and Hb A1a2 are adducts of sugar phosphates at the N-terminus of the beta chain. Hb A1b contains an unidentified nonphosphorylated sugar at the beta N-terminus. In addition, a significant portion of the major hemoglobin component (Hb Ao) is also glycosylated by a glucose ketoamine linkage at other sites on the molecule, including the N-terminus of the alpha chain and the epsilon-amino group of several lysine residues on both the alpha and the beta chains. The results indicate that the interaction of glucose and hemoglobin is rather nonspecific and suggests that other proteins are ...

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Protein-protein interactions and structural analysis of citrus proteins involved in transcriptional control during citrus canker disease

Spot 14 (S14) is a protein that is abundantly expressed in lipogenic tissues and is regulated in a manner similar to other enzymes involved in fatty acid synthesis. Deletion of S14 in mice decreased lipid synthesis in lactating mammary tissue, but the mechanism of S14's action is unknown. Here we present the crystal structure of S14 to 2.65 {angstrom} and biochemical data showing that S14 can form heterodimers with MIG12. MIG12 modulates fatty acid synthesis by inducing the polymerization and activity of acetyl-CoA carboxylase, the first committed enzymatic reaction in the fatty acid synthesis pathway. Coexpression of S14 and MIG12 leads to heterodimers and reduced acetyl-CoA carboxylase polymerization and activity. The structure of S14 suggests a mechanism whereby heterodimer formation with MIG12 attenuates the ability of MIG12 to activate ACC.

2011-09-06

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Temporal Correlations between Tick Abundance and Prevalence of Ticks Infected with Borrelia burgdorferi and Increasing Incidence of Lyme Disease

No abstract prepared.

2010-07-01

28

Linear and Nonlinear Effects of Habitat Structure on Composition and Abundance in the Macroinvertebrate Community of a ...

The abundance of host-seeking Ixodes scapularis nymphs, the principal vector for the Lyme disease agent, Borrelia burgdorferi, in Old Lyme, Lyme, and East Haddam, Connecticut,...Full Text Available

1998-05-01

29

Strontium and uranium concentrations in aragonite precipitated by some modern corals

... describe the relationship (Peake and Quinn, 1993). The power model was considered best if the R2 from this ... when stone abundance was low (Fig. 2). The power model best described the nonlinear relations...

30

Galactic deuterium abundance as a test of cosmological models

... Oceanographic Institution, Mass. (USA) v. 8 p. 439-442. abundance

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Cosmological implications of helium and deuterium abundances on Jupiter and Saturn

The problem on change of deuterium abundance in the process of galactic evolution (star evolution, supernova explosions, nucleosynthesis in supermassive objects) is considered. It is shown that the observable deuterium quantity in the interstellar medium must correspond to its cosmological abundance. This conclusion is independent of the rate of accretion of intergalactic gas by Galaxy. The effect of hypothetical pregalactic active objects on cosmological deuterium is small. It is poind out that observations of interstellar deuterium in absorbtion at lambda=91.6 cm are significant.

1982-02-01

32

Heat shock proteins as emerging therapeutic targets

The determination of relative abundances of helium and deuterium in Jupiter and Saturn provides estimates of the primordial abundances of these elements. The values so inferred can be compared with theoretical predictions of the standard model of the big-bang theory as a test of the model and suggest either that deuterium is more efficiently consumed in subsequent nucleosynthesis than hitherto assumed, or that the standard big-bang model should be revised in order for there to have been less production of primordial helium.

1983-03-17

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The numerical response of breeding Northern Saw-whet Owls Aegolius acadicus suggests nomadism

Chaperones (stress proteins) are essential proteins to help the formation and maintenance of the proper conformation of other proteins and to promote cell survival after a large variety of environmental...Full Text Available

2005-11-01

34

British Library Electronic Table of Contents (United Kingdom)

We used a 13-year time series of abundance estimates of breeding Northern Saw-whet Owls (Aegolius acadicus), and of small mammals from central Ontario, Canada, to assess the numerical response of the owls to small-mammal prey species. We found that the finite rate of increase of breeding owls was directly related to estimates of red-backed vole (Myodes gapperi) abundance. Thus, it appeared that the owls were nomadic, and made decisions about where to breed based on vole supply. The owls showed a much weaker response to deer mouse (Peromyscus maniculatus) abundance. Across all years, 55% of variation in owl rate of increase could be uniquely attributed to vole abundance, whereas only 3% could be attributed to mouse abundance. Consistent with the model of nomadism, there was only a weak rela...

2010-01-01

35

Abundance of West Nile virus mosquito vectors in relation to climate and landscape variables.

Charged nanoparticles as protein delivery systems: A feasibility study using lysozyme as model protein

It is currently unclear if the potential for West Nile virus transmission by mosquito vectors in the eastern United States is related to landscape or climate factors or both. We compared abundance of vector species between urban and suburban neighborhoods of Henrico County, VA, in relation to the following factors: temperature, precipitation, canopy cover, building footprint, and proximity to drainage infrastructure. Mosquitoes were collected throughout the 2005, 2006, and 2007 seasons and tested for West Nile virus (WNV) in pools of 10-50. Test results of mosquito pools were compared to average site abundance from 37 sites in Henrico County, VA; abundance was then examined in relation to ecological variables. Urban infrastructure was positively correlated with the abundance of Culex pipiens L./Cx. restuans, and our findings implicate combined sewer overflow systems as large contributors to Culex vector ...

2011-06-01

36

DEFF Research Database (Denmark)

Udgivelsesdato: 2008/5

2008-01-01

37

The Stability and Formation of Native Proteins from Unfolded Monomers Is Increased through Interactions with Unrelated Proteins

Localization of Reversion-Induced LIM Protein (RIL) in the Rat Central Nervous System

The intracellular concentration of protein may be as high as 400 mg per ml; thus it seems inevitable that within the cell, numerous protein-protein contacts are constantly occurring. A basic biochemical...Full Text Available

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A?(1-42) Aggregates into non-Toxic Amyloid Assemblies in the Presence of the Natural Polyphenol Oleuropein Aglycon.

Reversion-induced LIM protein (RIL) is a member of the ALP (actinin-associated LIM protein) subfamily of the PDZ/LIM protein family. RIL serves as an adaptor protein and seems to regulate cytoskeletons....Full Text Available

2009-02-28

39

The Effects of Exurbanization on Bird and Macro invertebrate Communities in Deciduous Forests on the Cumberland Plateau, Tennessee

Amyloid aggregation starts with the initial misfolding of peptide/protein precursors, with subsequent structural rearrangement into oligomers and protofibrils; the latter eventually organize into fibrils with shared basic structural features, found deposited in amyloid diseases. Mounting evidence indicates early oligomers as the most toxic amyloid species; accordingly, the search of inhibitors of their growth is considered a promising target to prevent amyloid toxicity. We recently showed that oleuropein aglycon, a polyphenol abundant in the extra virgin olive oil, interferes with the aggregation of amylin (involved in type-2 diabetes), eliminating its cytotoxicity. Here we report that oleuropein aglycon also hinders amyloid aggregation of A?(1-42) and its cytotoxicity, suggesting a general effect of such polyphenol. In particular, by using a wide panel of different spectroscopic, immunologic, cell viability and imaging techniques we provide a ...

2011-05-18

40

Optogalvanic isotope enrichment of Cu ions in Cu-Ne positive column discharges

To investigate the potential causes of changes to bird communities in exurban areas, we examined the relationship between bird and macro invertebrate communities in exurbanized forest. We randomly located sampling points across a gradient of exurbanization. We used point counts to quantify bird communities and sweep netting, soil cores, pitfalls, and frass collectors to quantify macro invertebrates. Bird communities had higher richness and abundance in exurban areas compared to undeveloped forests, and lost some species of conservation concern but gained others. The macro invertebrate community was slightly more abundant in exurban areas, with a slight shift in taxonomic composition. The abundance of macro invertebrates in soil cores (but not pitfalls) predicted the abundance of ground-foraging birds. The abundance of macro invertebrates in sweep nets was not associated with the ...

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The r-process in the early Galaxy

The isotopic enrichment of copper ions in a positive column Cu-Nu discharge using optogalvanic excitation is analyzed with a rate equation model With excitation at 510.6 nm, the fraction of the ions belonging to the 63-amu isotope of copper is enriched relative to the neutral abundance. Enrichment as large as 10% is calculated when the initial abundance of the neutral isotope is small (< or =0.1) and the discharge current density is large (> or =75 mA/cm/sup 2/). The degree of enrichment is examined as a function of the initial abundance, discharge current, the rate of charge exchange, and the diameter of the discharge tube.

1983-07-01

42

Foraging Behavior and Diet Preferences of a Released Population of Giant Tortoises in the Seychelles

We report Sr, Pd and Ag abundances for a sample of metal-poor field giants and analyze a larger sample of Y, Zr, and Ba abundances. The [Y/Zr] and [Pd/Ag] abundance ratios are similar to those measured for the r-process-rich stars CS 22892-052 and CS 31082-001. The [Pd/Ag] ratio is larger than predicted from the solar-system r-process abundances. The constant[Y/Zr] and [Sr/Y] values in the field stars places strong limits on the contributions of the weak s-process and the main s-process to the light neutron-capture elements. Stars in the globular cluster M 15 possess lower [Y/Zr] values than the field stars. There is a large dispersion in [Y/Ba]. Because the r-process is responsible for the production of the heavy elements in the early Galaxy, these dispersions require varying light-to-heavy ratios in r-process yields.

2002-01-01

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Diversity and Abundance of Hymenopterous Parasitoids Associated with Anastrepha fraterculus (Diptera: Tephritidae) in ...

... abundant species (S. urticifolia, M. dubious, T. procumbens, Ipomea sp., X. moluccensis, W. trilobata, Euphobia urta, and Paspalidium ... ...

44

Artificial and natural duplicates in pyrosequencing reads of metagenomic data

... Anastrepha fraterculus (Wiedemann) and Ceratitis capitata (Wiedemann), including Acca sellowiana (O. Berg) Burret, Campomanesia xanthocarpa O. Berg, Psidium ... ...

45

Analysis of Fisheries and Aquaculture

BackgroundArtificial duplicates from pyrosequencing reads may lead to incorrect interpretation of the abundance of species and genes in metagenomic studies. Duplicated reads were...Full Text Available

46

Abundance response of western European forest species along canopy openness and soil pH gradients

... abundance though an effect on escapement. The lobster aquaculture model continues to be updated as new biological ... become available. A monograph on the economics of aquaculture is near completion....

47

British Library Electronic Table of Contents (United Kingdom)

In order to better understand the structure and composition of forest plant communities, we aimed to predict the abundance of understory herbaceous species locally at the stand level and according to different environments. For this, we seeked to model species distributions of abundance at a regional scale in relationship with the local stand structure (canopy openness) and regional soil resources (soil pH). Floristic inventories, performed in different light and soil conditions located in 1202 records of north eastern France, were used to analyze the combined effect of canopy openness and soil pH on the abundance of 12 common western European forest species: Anemone nemorosa, Deschampsia flexuosa, Festuca altissima, Hedera helix, Lamium galeobdolon, Lonicera periclymenum, Molinia caerulea...

2011-01-01

48

Salt modulates the stability and lipid binding affinity of the adipocyte lipid-binding proteins

Protein misfolding disorders and macroautophagy

Adipocyte lipid-binding protein (ALBP or aP2) is an intracellular fatty acid-binding protein that is

2003-01-01

49

Misfolded Proteins and Retinal Dystrophies

A large group of diseases, termed protein misfolding disorders, share the common feature of the accumulation of misfolded proteins. The possibility of a common mechanism underlying either the pathogenesis...Full Text Available

2011-04-01

50

CAAX-box protein, prenylation process and carcinogenesis

Many mutations associated with retinal degeneration lead to the production of misfolded proteins by cells of the retina. Emerging evidence suggests that these abnormal proteins cause cell death...Full Text Available

2010-01-01

51

In Situ Detection, Isolation, and Physiological Properties of a Thin Filamentous Microorganism Abundant in Methanogenic Granular Sludges: a Novel Isolate Affiliated with a Clone Cluster, the Green Non-Sulfur Bacteria, Subdivision I

CAAX proteins are widely involved in global cellular functions such as proliferation, differentiation, and carcinogenesis. As an important modulator of biological activity, signal transduction via protein...Full Text Available

52

RIN4-like proteins mediate resistance protein-derived soybean defense against Pseudomonas syringae

We previously showed that very thin filamentous bacteria affiliated with the division green non-sulfur bacteria were abundant in the outermost layer of thermophilic methanogenic sludge granules fed...Full Text Available

2001-12-01

53

Protein S inherited qualitative deficiency: novel mutations and phenotypic influence.

Resistance (R) protein mediated recognition of pathogen avirulence effectors triggers signaling that induces a very robust form of species-specific immunity in plants. The soybean Rpg1-b protein mediates...Full Text Available

2010-11-01

54

Overcoming the challenges of membrane protein crystallography

PMID:20880255

2010-12-01

55

Effect of Garlic on the Digestion of Beef Protein during Storage

Membrane protein structural biology is still a largely unconquered area, given that approximately 25% of all proteins are membrane proteins and yet less than 150 unique structures are available. Membrane...Full Text Available

2008-10-01

56

A modified immunoblot method to identify substrates of protein kinases

Full Text Available

1991-10-01

57

LITHIUM ABUNDANCES IN RED GIANTS OF M4: EVIDENCE FOR ASYMPTOTIC GIANT BRANCH STAR POLLUTION IN GLOBULAR CLUSTERS?

Full Text Available

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Effective Control of Aquatic Invasive Species in Tropical Australia

The determination of Li and proton-capture element abundances in globular cluster (GC) giants allows us to constrain several key questions on the multiple population scenarios in GCs, from formation and early evolution to pollution and dilution mechanisms. In this Letter, we present our results on Li abundances for a large sample of giants in the intermediate-metallicity GC NGC 6121 (M4), for which Na and O have been already determined by Marino et al. The stars analyzed are both below and above the red giant branch bump luminosity. We found that the first and second generation stars share the same Li content, suggesting that a Li production must have occurred. This provides strong observational evidence supporting the scenario in which asymptotic giant branch stars are GC polluters.

2010-06-20

59

British Library Electronic Table of Contents (United Kingdom)

Often ecologists and natural resource managers can easily access data on invasive species occurrence across a region. Yet, collecting species abundance data over a large area is arguably more important for decision making, but inherently costly, so methods which can provide robust information at low-cost are particularly valuable. Studies of species distribution often use occurrence data to build models of the environmental niche. Environmental suitability derived from such models may be used to predict the potential distributions of species. The ability of such models to predict spatial patterns in abundance have recently been demonstrated. Here we tested the relationship of environmental suitability with local abundance of an aquatic invasive species, olive hymenachne (Hymenachne amplexi...

2011-01-01

60

Constraining SN Ia Models Using X-ray Spectra of Clusters of Galaxies

Kinetics of spherulite formation and growth: Salt and protein concentration dependence on proteins b-lactoglobulin and insulin

We present constraints on theoretical models of Type Ia SNe using spatially resolved ASCA X-ray spectroscopy of four galaxy clusters: Abell 496, Abell 2199, Abell 3571 & Perseus. All four clusters have central Fe abundance enhancements and an ensemble of abundance ratios are used to show that most of the Fe in the central regions of the clusters comes from SN Ia. At the center of each cluster, simultaneous analysis of spectra from all ASCA instruments shows that the Ni to Fe abundance ratio (normalized by the solar ratio) is ~ 4. We use the Ni/Fe ratio as a discriminator between SN Ia explosion models: the Ni/Fe ratio of ejecta from the "Convective Deflagration" model W7 is consistent with the observations, while those of "delayed detonation" models are not consistent at the 90% confidence level.

2000-01-01

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British Library Electronic Table of Contents (United Kingdom)

Proteins aggregated into spherulite structures of amyloid fibrils have been observed in patients with certain brain diseases such as Alzheimers and Parkinsons. The conditions under which these protein spherulites form and grow are not currently known. In order to illuminate the role of environmental factors on protein spherulites, this research aims to explore the kinetics and mechanisms of spherulite formation and growth, as monitored by optical microscopy, in a range of salt concentrations, and initial protein concentrations for two model proteins: bovine b-lactoglobulin and insulin. These two proteins are significantly different in their size and fibril growth rate, but both of these proteins have been shown previously to form amyloid fibrils and spherulites under low pH conditions. The...

2009-01-01

62

Vitamin E Is Essential for Seed Longevity and for Preventing Lipid Peroxidation during Germination

USGS OFR 2010-1181: Kittlitz's and Marbled Murrelets in Kenai...

Tocopherols (vitamin E) are lipophilic antioxidants synthesized by all plants and are particularly abundant in seeds. Despite cloning of the complete suite of tocopherol biosynthetic enzymes and successful...Full Text Available

2004-06-01

63

Role of Calcitonin Gene-Related Peptide in Bone Repair after Cyclic Fatigue Loading

Report 2010-1181 >> Pubs Warehouse >OFR 2010-1181 Kittlitz's and Marbled Murrelets in Kenai Fjords National Park, South-Central Alaska: At-Sea Distribution, Abundance, and...

2011-08-20

64

Predictors for presence and abundance of small mammals in households of villages endemic for commensal rodent plague in Yunnan Province, China

BackgroundCalcitonin gene related peptide (CGRP) is a neuropeptide that is abundant in the sensory neurons which innervate bone. The effects of CGRP on isolated bone cells have been...Full Text Available

65

New strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice.

BackgroundNinety-one rodent plague epidemics have occurred in Lianghe county, Yunnan Province, China, between 1990 and 2006. This study aimed to identify predictors for the presence...Full Text Available

66

New approach to study fast and slow motions in lipid bilayers: application to dimyristoylphosphatidylcholine-cholesterol interactions.

A new strain of mouse hepatitis virus (MHV) was isolated from pooled gut suspensions from an epizootic of lethal enteritis in newborn mice. Negative-contrast electron microscopy showed an abundance...Full Text Available

1979-05-01

67

Natural mutagenesis of human genomes by endogenous retrotransposons

Natural abundance 13C solid-state nuclear magnetic resonance spectroscopy was used to investigate the effect of the incorporation of cholesterol on the dynamics of dimyristoylphosphatidylcholine (DMPC)...Full Text Available

1995-05-01

68

Metabolic labeling and direct imaging of choline phospholipids in vivo

SUMMARYTwo abundant classes of mobile elements, namely Alu and L1 elements, continue to generate new retrotransposon insertions in human genomes. Estimates suggest that these...Full Text Available

2010-06-25

69

Malignant Catarrhal Fever of Cattle Is Associated with Low Abundance of IL-2 Transcript and a Predominantly Latent Profile of Ovine Herpesvirus 2 Gene Expression

Choline (Cho)-containing phospholipids are the most abundant phospholipids in cellular membranes and play fundamental structural as well as regulatory roles in cell metabolism and signaling. Although...Full Text Available

2009-09-08

70

Impact of sphingomyelin levels on coronary heart disease and left ventricular systolic function in humans

BackgroundMalignant catarrhal fever (MCF) is a lethal disease of cattle, characterized by vasculitis, necrosis, and accumulation of activated, dysregulated cytotoxic lymphocytes...Full Text Available

71

If I Had - Signs of Aging Skin

Sphingomyelin (SM) is an abundant phospholipid in cell membranes and in lipoproteins. In human plasma, SM is mainly found in atherogenic lipoproteins; therefore, higher levels of SM may promote atherogenesis....Full Text Available

72

Medline Plus

... also include a healthy diet that included abundant antioxidants, such as vitamin C, vitamin E, and lycopene. ... exfoliants, the higher-level moisturizers as well as antioxidants as my night product. A very rich moisturizer ...

73

HCN polymers characterized by solid state NMR: Chains and sheets formed in the neat liquid

Community structure and temporal variability of juvenile fish assemblages in natural and replanted mangroves, Sonneratia alba Sm., of Gazi Bay, Kenya

Hydrogen cyanide polymerizes readily under a variety of conditions and significant prebiotic roles have been suggested for these polymers due to the abundance of HCN in universe. However, the structures...Full Text Available

2009-04-07

74

British Library Electronic Table of Contents (United Kingdom)

The juvenile fish community associated with natural, degraded and replanted Sonneratia alba mangroves in Gazi Bay was sampled during the South East and North East monsoons between April 2002 and June 2003. A total of 1800 individuals belonging to 49 taxa and 34 families were collected from the intertidal forest using stake nets. Fish abundance ranged from 0.93+-0.20ind. m-2 (SEM02) to 1.16+-0.18ind. m-2 (SEM03) between seasons and between 0.54+-0.07ind. m-2 and 1.64+-0.33ind. m-2 for individual sites across seasons. Five taxa accounted for approximately 70% of the total fish abundance, with Gobidae and Gerres oyena dominating. ANOSIM revealed seasonal differences in fish species composition and abundance (p=0.01) due to fluctuating abundances of primarily Terapon sp. and Thryssa sp. The ma...

2007-01-01

75

Chemical Compositions of a sample of candidate post-AGB stars

Characterization of Airborne Microbial Communities at a High-Elevation Site and Their Potential To Act as Atmospheric Ice Nuclei?

We have derived elemental abundances for a sample of nine IRAS sources with colours similar to those of post-AGB stars. For IRAS 01259+6823, IRAS 05208-2035, IRAS 04535+3747 and IRAS 08187-1905 this is the first detailed abundance analysis based upon high resolution spectra. Mild indication of s-processing for IRAS 01259+6823, IRAS 05208-2035 and IRAS 08187-1905 have been found and a more comprehensive study of s-process enhanced objects IRAS 17279-1119 and IRAS 22223+4327 have been carried out. We have also made a contemporary abundance analysis of the high galactic latitude supergiants BD+39 4926 and HD 107369. The former is heavily depleted in refractories and estimated [Zn/H] of -0.7 dex most likely gives initial metallicity of the star. For HD 107369 the abundances of alpha and Fe-peak elements are similar to those of halo objects and moderate deficiency of s-process elements is seen. IRAS ...

2011-01-01

76

Cerebrospinal Fluid Steroidomics: Are Bioactive Bile Acids Present in Brain?*

Bacteria and fungi are ubiquitous in the atmosphere. The diversity and abundance of airborne microbes may be strongly influenced by atmospheric conditions or even influence atmospheric conditions themselves...Full Text Available

2009-08-01

77

Boundary conditions for the paleoenvironment: chemical and physical processes in the pre-solar nebula

In this study we have profiled the free sterol content of cerebrospinal fluid by a combination of charge tagging and liquid chromatography-tandem mass spectrometry. Surprisingly, the most abundant cholesterol...Full Text Available

2010-02-12

78

Two additional hyperfine components of the interstellar radical C3H were detected. In addition, methanol was discovered in interstellar clouds. The abundance of HCCN and various chemical isomers in molecular clouds was investigated.

1985-02-01

79

Big bang nucleosynthesis

Aluminium. Health and Environmental Hazards.

The concordance of standard big bang nucleosynthesis theory and the related observations of the light element isotopes (including some new higher {sup 4}He abundances) will be reviewed. Implications of BBN on chemical evolution, dark matter and constraints on particle properties will be discussed.

1999-01-01

80

A highly dispersed limonite catalyst for direct liquefaction of brown coal

Aluminium is an abundant metal consisting about 8 per cent of the earth's crust. It has long been considered as essentially nontoxic and nonabsorbable from the gastrointestinal tract. More recent studies have, however, due to better analytical capabilitie...

1982-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

81

The use of lead isotopic abundances in trace uranium samples for nuclear forensics analysis

A highly dispersed limonite catalyst was shown to have advantageous properties in a commercial liquefaction plant: it has high catalytic activity, low cost for catalyst production and abundant resources. 3 refs., 3 figs., 2 tabs.

1999-07-01

82

Beta-decay half-lives of new neutron-rich chromium-to-nickel isotopes and their consequences for the astrophysical r-process

Secondary ion mass spectrometry (SIMS), secondary electron microscopy (SEM) and X-ray analysis have been applied to the measurement of U-bearing particles with the intent of gleaning information concerning their history and/or origin. The lead isotopic abundances are definitive indicators that U-bearing particles have come from an ore-body, even if they have undergone chemical processing. SEM images and X-ray analysis can add further information to the study that may allude to the extent of chemical processing. The presence of 'common' lead that does not exhibit a radiogenic signature is clear evidence of anthropogenic origin. (author)

2010-06-01

83

Whey protein isolate attenuates strength decline after eccentrically-induced muscle damage in healthy individuals

Beta-decay studies of the new neutron-rich isotopes sup(58,59)Cr, "6"3Mn, sup(66,67)Co and "6"9Ni, yielding distinctly shorter half-lives than the corresponding theoretical predictions, are presented. The influence of the short half-lives on the r-process calculations of nuclear abundances is discussed. It is concluded that a significantly higher neutron density than the one obtained from explosive helium burning in supernovae is needed to reproduce the observed abundances around A = 80. (orig.).

84

Transcriptional repression and developmental functions of the atypical vertebrate GATA protein TRPS1

BackgroundWe examined the effects of short-term consumption of whey protein isolate on muscle proteins and force recovery after eccentrically-induced muscle damage in healthy individuals.MethodsSeventeen...Full Text Available

85

Thioredoxin Is an Essential Protein Induced by Multiple Stresses in Bacillus subtilis

Known vertebrate GATA proteins contain two zinc fingers and are required in development, whereas invertebrates express a class of essential proteins containing one GATA-type zinc finger. We isolated...Full Text Available

2001-04-02

86

The protein storage vacuole

Thioredoxin, a small, ubiquitous protein which participates in redox reactions through the reversible oxidation of its active center dithiol to a disulfide, is an essential protein in Bacillus...Full Text Available

1998-04-01

87

The p14 FAST Protein of Reptilian Reovirus Increases Vesicular Stomatitis Virus Neuropathogenesis?

Storage proteins are deposited into protein storage vacuoles (PSVs) during plant seed development and maturation and stably accumulate to high levels; subsequently, during germination the storage...Full Text Available

2001-12-10

88

Strategies for the uses of lanthanide NMR shift probes in the determination of protein structure in solutio. Application to the EF calcium binding site of carp parvalbumin.

The fusogenic orthoreoviruses express nonstructural fusion-associated small transmembrane (FAST) proteins that induce cell-cell fusion and syncytium formation. It has been speculated that the FAST proteins...Full Text Available

2009-01-01

89

Stage-specific protein synthesis during early embryogenesis in Drosophila melanogaster.

The homologous sequences observed for many calcium binding proteins such as parvalbumin, troponin C, the myosin light chains, and calmodulin has lead to the hypothesis that these proteins have homologous...Full Text Available

1980-10-01

90

Serum protein binding and the role of increased alpha 1-acid glycoprotein in moderately obese male subjects.

The changes in protein species synthesized during early Drosophila embryogenesis were characterized by two-dimensional electrophoresis. Of the 261 proteins scored, 68 (26%) show dramatic changes in...Full Text Available

1983-01-01

91

Selecting Folded Proteins from a Library of Secondary Structural Elements

Serum protein and lipid concentrations as well as the serum protein binding of propranolol, diazepam and phenytoin were measured in normal weight and obese volunteers. Concentrations of alpha 1-acid...Full Text Available

1984-12-01

92

Regulation of the Actin Cytoskeleton by an Interaction of IQGAP Related Protein GAPA with Filamin and Cortexillin I

A protein evolution strategy is described by which double-stranded DNA fragments encoding defined E. coli protein secondary structural elements (α-helices, β-strands...Full Text Available

2008-01-09

93

Regulation of ROMK1 Channels by Protein-tyrosine Kinase and -tyrosine Phosphatase*

Filamin and Cortexillin are F-actin crosslinking proteins in Dictyostelium discoideum allowing actin filaments to form three-dimensional networks. GAPA, an IQGAP related protein, is...Full Text Available

94

Purification, characterization and binding interactions of the Chinese-cobra (Naja naja atra) serum antitoxic protein CSAP.

We have used the two-electrode voltage clamp technique and the patch clamp technique to investigate the regulation of ROMK1 channels by protein-tyrosine phosphatase (PTP) and protein-tyrosine...Full Text Available

2001-03-09

95

Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent.

The characterization of the single-chain protein in Chinese-cobra (Naja naja atra) blood serum, which yields strong specific protection against the venom of the same snake, is reported. The protein,...Full Text Available

1993-07-15

96

Protein Damage by Reactive Electrophiles: Targets and Consequences

A heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate, was synthesized. Its N-hydroxysuccinimide ester group reacts with amino groups and the 2-pyridyl disulphide structure reacts...Full Text Available

1978-09-01

97

ProMoST: A tool for calculating the pI and molecular mass of phosphorylated and modified proteins on 2 dimensional gels

It has been sixty years since the Millers first described the covalent binding of carcinogens to tissue proteins. Protein covalent binding was gradually overshadowed by the emergence of DNA...Full Text Available

2008-01-01

98

Partial Characterization of Cadmium-Binding Protein from Roots of Tomato ¹

Protein modifications such as phosphorylation are often studied by two-dimensional gel electrophoresis since the perturbation in the protein’s pI value is readily detected by this method....Full Text Available

2009-01-01

99

PDBe: Protein Data Bank in Europe

Cd-binding protein was extracted from tomato roots and purified on QAE-Sephadex A-25 and on Sephadex G-75 in 1 molar KCl buffer. The protein preparation was light brown and contained predominantly Cd...Full Text Available

1986-07-01

100

Nucleocapsid and Matrix Protein Contributions to Selective Human Immunodeficiency Virus Type 1 Genomic RNA Packaging

The Protein Data Bank in Europe (PDBe; pdbe.org) is actively involved in managing the international archive of biomacromolecular structure data as one of the partners in the Worldwide Protein Data Bank...Full Text Available

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

101

The nucleocapsid protein (NC) of retroviruses plays a major role in genomic RNA packaging, and some evidence has implicated the matrix protein (MA) of certain retroviruses in viral RNA binding. To further...Full Text Available

1998-03-01

102

NASCENT: An automatic protein interaction network generation tool for non-model organisms

The activity of Src-related protein-tyrosine kinases is repressed by the phosphorylation of a conserved carboxyl-terminal tyrosine by another cytoplasmic protein-tyrosine kinase termed p50csk. In this...Full Text Available

1994-05-24

103

Modular coherence of protein dynamics in yeast cell polarity system

Large quantity of reliable protein interaction data are available for model organisms in public depositories (e.g., MINT, DIP, HPRD, INTERACT). Most data correspond to experiments with the proteins...Full Text Available

104

Mammalian end binding proteins control persistent microtubule growth

In this study, we investigated on a systems level how complex protein interactions underlying cell polarity in yeast determine the dynamic association of proteins with the polar cortical domain (PCD)...Full Text Available

2011-05-03

105

How DNA coiling enhances target localization by proteins

End binding proteins (EBs) are highly conserved core components of microtubule plus-end tracking protein networks. Here we investigated the roles of the three mammalian EBs in controlling microtubule...Full Text Available

2009-03-09

106

Expression and Characterization of the Mycobacterium tuberculosis Serine/Threonine Protein Kinase PknB

Many genetic processes depend on proteins interacting with specific sequences on DNA. Despite the large excess of nonspecific DNA in the cell, proteins can locate their targets rapidly. After initial...Full Text Available

2008-10-14

107

Ethylene Regulates Monomeric GTP-Binding Protein Gene Expression and Activity in Arabidopsis1

PknB is a member of the newly discovered eukaryotic-like protein serine/threonine kinase (PSTK) family of proteins. The pknB gene was cloned and expressed in Escherichia coli....Full Text Available

1999-11-01

108

Effect of Protein Binding on the Pharmacological Activity of Highly Bound Antibiotics?

Ethylene rapidly and transiently up-regulates the activity of several monomeric GTP-binding proteins (monomeric G proteins) in leaves of Arabidopsis as determined by two-dimensional gel electrophoresis...Full Text Available

2003-04-01

109

Coat protein gene duplication in a filamentous RNA virus of plants.

During antibiotic drug development, media are frequently spiked with either serum/plasma or protein supplements to evaluate the effect of protein binding. Usually, previously reported serum or plasma...Full Text Available

2008-11-01

110

Cardiac Myosin Is a Substrate for Zipper-interacting Protein Kinase (ZIPK)*

Computer-assisted analysis revealed a striking sequence similarity between the putative 24-kDa protein (p24) encoded by open reading frame (ORF) 5 of beet yellows closterovirus and the coat protein...Full Text Available

1992-10-01

111

Biosynthesis and characterization of rabbit tooth enamel extracellular-matrix proteins.

Zipper-interacting protein kinase (ZIPK) is a member of the death-associated protein kinase family associated with apoptosis in nonmuscle cells where it phosphorylates myosin regulatory light chain...Full Text Available

2010-02-19

112

Being a binding site: Characterizing residue composition of binding sites on proteins

Tooth enamel biomineralization is mediated by enamel proteins synthesized by ameloblast cells. Two classes of proteins have been described: enamelins and amelogenins. In lower vertebrates the absence...Full Text Available

1988-05-01

113

Analyzing the simplicial decomposition of spatial protein structures

The Protein Data Bank contains the description of more than 45,000 three-dimensional protein and nucleic-acid structures today. Started to exist as the computer-readable depository of crystallographic...Full Text Available

114

An Analysis of the Subunit Structure of the Crystalloid Protein Complex from Castor Bean Endosperm 1

BackgroundThe fast growing Protein Data Bank contains the three-dimensional description of more than 45000 protein- and nucleic-acid structures today. The large majority of the data...Full Text Available

115

Alpha-Hemoglobin-Stabilizing Protein: An Erythroid Molecular Chaperone

Chromatographic and electrophoretic studies have shown that the subunits of the crystalloid protein, isolated from mature castor bean (Ricinus communis L. cv Hale) seed endosperm protein...Full Text Available

1983-06-01

116

The structure of receptor-associated protein (RAP)

Alpha-hemoglobin-stabilizing protein (AHSP) is an erythroid-specific protein that acts as a molecular chaperone for the free α chains of hemoglobin. Evidence strongly suggests...Full Text Available

2011-01-01

117

Phospholemman: A Novel Cardiac Stress Protein

The receptor-associated protein (RAP) is a molecular chaperone that binds tightly to certain newly synthesized LDL receptor family members in the endoplasmic reticulum (ER) and facilitates their delivery...Full Text Available

2007-08-01

118

PDBpaint, a visualization webservice to tag protein structures with sequence annotations

Phospholemman (PLM), a member of the FXYD family of regulators of ion transport, is a major sarcolemmal substrate for protein kinases A and C in cardiac and skeletal muscle. In the heart, PLM...Full Text Available

2010-08-01

119

British Library Electronic Table of Contents (United Kingdom)

Summary: Protein features are often displayed along the linear sequence of amino acids that make up that protein, but in reality these features occupy a position in the folded proteins 3D space. Mapping sequence features to known or predicted protein structures is useful when trying to deduce the function of those features and when evaluating sequence or structural predictions. To facilitate this goal, we developed PDBpaint, a simple tool that displays protein sequence features gathered from bioinformatics resources on top of protein structures, which are displayed in an interactive window (using the Jmol Java viewer). PDBpaint can be used either with existing protein structures or with novel structures provided by the user. The current version of PDBpaint allows the visualization of annot...

2011-01-01

120

Male Reproductive Proteins and Reproductive Outcomes

Biogenesis of the Protein Storage Vacuole Crystalloid

Male reproductive proteins (MRPs), associated with sperm and semen, are the moieties responsible for carrying male genes into the next generation. Evolutionary biologists have focused on their...Full Text Available

2008-06-01

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121

Alpha-1 antitrypsin is a potential biomarker for hepatitis B

We identify new organelles associated with the vacuolar system in plant cells. These organelles are defined biochemically by their internal content of three integral membrane proteins: a chimeric reporter...Full Text Available

2000-08-21

122

9902631 - Lysozyme - NASA

BackgroundFunction exertion of specific proteins are key factors in disease progression, thus the systematical identification of those specific proteins is a prerequisite to understand...Full Text Available

123

Thermal decomposition of gamma irradiated proteins by thermal analysis methods

Protein isolated from hen egg-white and functions as a bacteriostatic enzyme by degrading bacterial cell walls. First enzyme ever characterized by protein ...

124

The hrpK Operon of Pseudomonas syringae pv. tomato DC3000 Encodes Two Proteins Secreted by the Type III (Hrp) Protein Secretion System: HopB1 and HrpK, a Putative Type III Translocator

Short communication. 2 figs.

1996-12-31

125

Temporal Fluctuations of Fluorescence Energy Transfer between TwoDyes Conjugated to a Single Protein

Pseudomonas syringae is a gram-negative bacterial plant pathogen that is dependent on a type III protein secretion system (TTSS) and the effector proteins it translocates into plant...Full Text Available

2005-01-01

126

Structure and Function of NtCDPK1, a Calcium-dependent Protein Kinase in Tobccco

No abstract prepared.

1999-01-04

127

Protein profile changes in platelet concentrates according to storage and leukoreduction- analysis using proteomics technology

Full Text Available

2000-05-01

128

Moessbauer studies of the molybdenum-iron-protein in the nitrogenase of Xanthobacter autotrophicus GZ 29. Moessbaueruntersuchungen am Molybdaen-Eisen-Protein der Nitrogenase von Xanthobacter Autotrophicus GZ 29

Full Text Available

2008-02-01

129

METHOD FOR SYNTHESIZING TAURINE FROM PROTEIN-CONTAINING MATERIAL BY USING HIGH-TEMPERATURE HIGH-PRESSURE WATER

Biological cells of the nitrogen fixating bacteria Xanthobacter autotrophicus GZ 29 have been cultivated with 57-Fe, and from the nitrogenase of these cells the 57-Fe marked molybdenum-iron-protein is extracted. The Moessbauer spectra of this enzyme show that the structure of the iron cluster is similar to the structure in the molybdenum-iron-proteins of Azotobacter vinelandii, Klebsiella pneumoniae or Clostridium pasteurianum.

1983-05-01

130

J-STORE (Japan)

Full Text Available

2003-08-28

131

High Throughput Screening on Angiogenesis Inhibitor and Promoter of Medicinal Plants using a Protein Microarray Chip

Expression of microtubule associated protein of low molecular weight (TAU) in mouse brain development

Full Text Available

2007-04-01

132

Effect of the Toluene Administration on the Liver Damage in Rats Fed Low or Standard Protein Diet

Published in summary form only.

133

Effect of nitric oxide on endoplasmic reticulum calcium homeostasis, protein synthesis and energy metabolism

Full Text Available

1996-12-01

134

DEFF Research Database (Denmark)

ATPase, endoplasmic reticulum, calcium, ischaemia

2000-01-01

135

Effect of Dietary Protein on the Changes of Lipoprotein Fractions in Carbon Tetrachloride-Treated Rats

Effect of Dietary Energy, Protein on Growth and Blood Composition of Cross Bred Chicks

Full Text Available

1993-04-01

136

Effect of Angiotensin-I Converting Enzyme Inhibitory from Hydrolysate of Soybean Protein Isolate

Full Text Available

2008-09-01

137

Dyeability and Functionality of Pine Needles Extract (Part II) -Dyeing Properties of Protein Fiber-

Full Text Available

2010-01-01

138

Deposition of Matrix and Crystalloid Storage Proteins during Protein Body Development in the Endosperm of Ricinus communis L. cv. Hale Seeds ¹

Full Text Available

2011-04-01

139

Comparative Study on Protein and Amino Acid Composition of Wild and Cultured Eel

Protein bodies within the endosperm of castor bean (Ricinus communis L. cv. Hale) seeds arise from numerous small vacuoles which progressively become filled with storage protein, of...Full Text Available

1982-06-01

140

Biochemical characterization of Alanine racemase- a spore protein produced by Bacillus anthracis

Full Text Available

1986-01-01

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141

Bilateral retinal dysplasia and secondary glaucoma associated with homozygous protein C deficiency.

Full Text Available

2009-01-01

142

Effect of host nutrition on immunity and local immune response of rabbits to Obeliscoides cuniculi

Full Text Available

2005-06-01

143

Bacteriophage P22 capsid size determination: Roles for the coat protein telokin-like domain and the scaffolding protein amino-terminus

In a series of experiments carried out on young and adult rabbits the effect of isocaloric low protein diets containing 4% or 8% protein compared with a diet containing 21% protein on Obeliscoides cuniculi infection was studied. The pathogenesis, resistance and local immunity were assessed after single infections with 10,000 larvae or reinfection with 5000 larvae. Live weight gain was reduced in young and adult rabbits fed the low protein diets, but the establishment of parasites was not substantially influenced by protein deprivation. However, development of worms in the histotrophic phase and parasite fecundity were impaired in association with the low protein diet. Moreover, mild anaemia as well as changes in the mucosal immune response as a result of infection were related to the level of dietary protein. (author). 30 refs, 6 figs, 5 ...

1987-05-11

144

British Library Electronic Table of Contents (United Kingdom)

Assembly of icosahedral capsids of proper size and symmetry is not understood. Residue F170 in bacteriophage P22 coat protein is critical for conformational switching during assembly. Substitutions at this site cause assembly of tubes of hexamerically arranged coat protein. Intragenic suppressors of the ts phenotype of F170A and F170K coat protein mutants were isolated. Suppressors were repeatedly found in the coat protein telokin-like domain at position 285, which caused coat protein to assemble into petite procapsids and capsids. Petite capsid assembly strongly correlated to the side chain volume of the substituted amino acid. We hypothesize that larger side chains at position 285 torque the telokin-like domain, changing flexibility of the subunit and intercapsomer contacts. Thus, a sing...

2011-01-01

145

Relic abundance of WIMPs in non-standard cosmological scenarios

Herschel observations in the ultracompact HII region Mon R2: Water in dense Photon-dominated regions (PDRs)

In this thesis we study the relic density n{sub {chi}} of non--relativistic long--lived or stable particles {chi} in various non--standard cosmological scenarios. First, we discuss the relic density in the non--standard cosmological scenario in which the temperature is too low for the particles {chi} to achieve full chemical equilibrium. We also investigated the case where {chi} particles are non--thermally produced from the decay of heavier particles in addition to the usual thermal production. In low temperature scenario, we calculate the relic abundance starting from arbitrary initial temperatures T{sub 0} of the radiation--dominated epoch and derive approximate solutions for the temperature dependence of the relic density which can accurately reproduces numerical results when full thermal equilibrium is not achieved. If full equilibrium is reached, our ansatz no longer reproduces the correct temperature dependence of the {chi} number density. However, we can ...

2007-08-06

146

Identification of proteins important for tetracycline (TC) binding to ribosomes by single protein omission reconstitution (SPORE) experiments

Mon R2, at a distance of 830 pc, is the only ultracompact HII region (UC HII) where the photon-dominated region (PDR) between the ionized gas and the molecular cloud can be resolved with Herschel. HIFI observations of the abundant compounds 13CO, C18O, o-H2-18O, HCO+, CS, CH, and NH have been used to derive the physical and chemical conditions in the PDR, in particular the water abundance. The 13CO, C18O, o-H2-18O, HCO+ and CS observations are well described assuming that the emission is coming from a dense (n=5E6 cm-3, N(H2)>1E22 cm-2) layer of molecular gas around the UC HII. Based on our o-H2-18O observations, we estimate an o-H2O abundance of ~2E-8. This is the average ortho-water abundance in the PDR. Additional H2-18O and/or water lines are required to derive the water abundance profile. A lower density envelope (n~1E5 cm-3, N(H2)=2-5E22 cm-2) is responsible for the ...

2010-01-01

147

Protein expression in white spot syndrome virus infected Penaeus monodon fabricius.

TC inhibits protein synthesis in E. coli by interfering with aminoacyl-tRNA binding to the ribosomal A site, and there is strong evidence that such inhibition results from TC binding to a high affinity site on the 30S subunit. The SPORE approach has been used to define those 30S proteins that are potentially important for high affinity TC binding, measured as the (/sup 3/H)-TC co-sedimenting with the reconstitution particle through a sucrose density gradient. Reverse phase-HPLC has been used both to prepare ribosomal proteins and to analyze the protein content of reconstituted particles. The results obtained so far show that protein S7, as well as some proteins linked to S7 in the 30S assembly map, are important for TC binding, whereas other ribosomal proteins are not. These results are in very good accord with their earlier photoaffinity ...

1987-05-01

148

Open clusters as key tracers of Galactic chemical evolution. III. Element abundances in Berkeley 20, Berkeley 29, Collinder 261, and Melotte 66

White spot syndrome virus (WSSV) is the causative agent of the white spot disease of shrimp. Penaeus monodon were captured from Muttukadu Estuary in Chennai, India, transported to the laboratory and maintained in an aerated system with continuous water circulation-biofiltration. WSSV-free P. monodon were challenged by feeding them only once with WSSV-infected tissues of P. monodon. Cumulative mortality (100%) of the infected individuals was determined. Tissues from infected and uninfected shrimp such as muscles, hepatopancreas, heart, gills and eye tissues (100mg of each) and haemolymph (50 microl) were subjected to SDS-PAGE. In infected muscle tissue, six newly expressed proteins were detected. In infected haemolymph, four new proteins and three intensely expressed high molecular weight proteins were observed. Three intensely expressed high molecular weight proteins were detected in infected heart ...

2005-06-01

149

Abundances of Jupiter's Trace Hydrocarbons From Voyager and Cassini

Galactic open clusters are since long recognized as one of the best tools for investigating the radial distribution of iron and other metals. We employed FLAMES at VLT to collect UVES spectra of bright giant stars in a large sample of open clusters, spanning a wide range of Galactocentric distances, ages, and metallicities. We present here the results for four clusters: Berkeley 20 and Berkeley 29, the two most distant clusters in the sample; Collinder 261, the oldest and the one with the minimum Galactocentric distance; Melotte 66. Equivalent width analysis was carried out using the spectral code MOOG and Kurucz model atmospheres to derive abundances of Fe, Al, Mg, Si, Ca, Ti, Cr, Ni, Ba; non-LTE Na abundances were derived by direct line-profile fitting. We obtain subsolar metallicities for the two anticenter clusters Be 20 ([Fe/H]=-0.30, rms=0.02) and Be 29 ([Fe/H]=-0.31, rms=0.03), and for Mel 66 ([Fe/H]=-0.33, rms=0.03), located in the ...

2008-01-01

150

Stellar Pollution in the Solar Neighborhood

The flybys of Jupiter by the Voyager spacecraft in 1979, and over two decades later by Cassini in 2000, have provided us with unique datasets from two different epochs, allowing the investigation of seasonal change in the atmosphere. In this paper we model zonal averages of thermal infrared spectra from the two instruments, Voyager 1 IRIS and Cassini CIRS, to retrieve the vertical and meridional profiles of temperature, and the abundances of the two minor hydrocarbons, acetylene (C2H2) and ethane (C2H6). The spatial variation of these gases is controlled by both chemistry and dynamics, and therefore their observed distribution gives us an insight into both processes. We find that the two gases paint quite different pictures of seasonal change. Whilst the 2-D cross-section of C2H6 abundance is slightly increased and more symmetric in 2000 (northern summer solstice) compared to 1979 (northern fall equinox), the major trend of equator to pole ...

2010-01-01

151

Source and profile of paralytic shellfish poisoning toxins in shellfish in Daya Bay, South China Sea

We study spectroscopically determined iron abundances of 642 solar-type stars to search for the signature of accreted iron-rich material. We find that the metallicity [Fe/H] of a subset of 466 main sequence stars, when plotted as a function of stellar mass, mimics the pattern seen in lithium abundances in open clusters. Using Monte Carlo models we find that, on average, these stars have accreted about 0.4 Earth masses of iron while on the main sequence. A much smaller sample of 19 stars in the Hertzsprung gap, which are slightly evolved and whose convection zones are significantly more massive, have lower average [Fe/H], and their metallicity shows no clear variation with stellar mass. These findings suggest that terrestrial-type material is common around solar type stars.

2000-01-01

152

British Library Electronic Table of Contents (United Kingdom)

Changes in cell density and cyst flux of Alexandrium tamarense, paralytic shellfish poisoning (PSP) toxin contents in shellfishes, and environmental parameters were measured in two stations in Daya Bay, South China Sea from March 2005 to July 2006. Vegetative cells of A. tamarense occurred sporadically; however, they presented abundantly during the winter months. Meanwhile, cyst flux reached its maximum level just following the peak abundance of motile cells. The PSP contents in shellfish were generally low, but higher in winter with the maximum of 14,015 @mg STX equiv./kg. The majority of toxins were found in digestive glands, with a maximum of 66,227 @mg STX equiv./kg. There were significant positive relationships between toxin level and vegetative cell density and cyst flux. This indica...

2011-01-01

153

Seasonal and habitat abundance and distribution of some forensically important blow flies (Diptera: Calliphoridae) in Central California

British Library Electronic Table of Contents (United Kingdom)

Seasonal and habitat calliphorid abundance and distribution were examined weekly for two years (2001-2003) in Santa Clara County, California, using sentinel traps baited with bovine liver. Of the 34,389 flies examined in three defined habitats (rural, urban, and riparian), 38% of the total catch represented Compsomyiops callipes (Bigot) and 23% represented Phormia regina (Meigen). Other flies collected in this survey included Calliphora vomitoria (Linnaeus), Calliphora latifrons (Hough), Lucilia sericata (Meigen), Lucilia cuprina (Wiedemann), and Lucilia mexicana (Macquart), which is a new record for the area. Multivariate MANOVA and ANOVA (P 0.05) analysis indicate significant seasonal habitat preference for all fly species examined. This information may be used to identify potentially fo...

2011-01-01

154

People of the ancient rainforest: Late Pleistocene foragers at the Batadomba-lena rockshelter, Sri Lanka

British Library Electronic Table of Contents (United Kingdom)

Batadomba-lena, a rockshelter in the rainforest of southwestern Sri Lanka, has yielded some of the earliest evidence of Homo sapiens in South Asia. H. sapiens foragers were present at Batadomba-lena from ca. 36,000 cal BP to the terminal Pleistocene and Holocene. Human occupation was sporadic before the global Last Glacial Maximum (LGM). Batadomba-lena's Late Pleistocene inhabitants foraged for a broad spectrum of plant and mainly arboreal animal resources (monkeys, squirrels and abundant rainforest snails), derived from a landscape that retained equatorial rainforest cover through periods of pronounced regional aridity during the LGM. Juxtaposed hearths, palaeofloors with habitation debris, postholes, excavated pits, and animal and plant remains, including abundant Canarium nutshells, ref...

2011-01-01

155

Lithium abundances of southern F, G, and K dwarfs

Investigations into the early life history of naturally produced spring chinook salmon and summer steelhead in the Grande Ronde River Basin : annual report 2000 : project period 1 October 1999 to 30 November 2000.; ANNUAL

Observations are reported of the lithium feature (6708 A) in some bright southern stars, most of which are F, G, or K dwarfs. Three of these stars have been suggested as belonging to the Ursa Major Group. Two of these three have the large Li abundance and strong Ca II H and K emission expected of such young stars. The third potential Ursa Major Group member has little Li, but is also not a true kinematic member. No stars were found with abnormal (Li-6)/(Li-7) ratios, in accord with other recent analyses. Thus it appears that all stars have (Li-6)/(Li-7) near the solar/terrestrial value of 0.08. 31 references.

1985-01-01

156

Introduction to Big Bang nucleosynthesis: open and closed models, anisotropies

The authors determined migration timing and abundance of juvenile spring chinook salmon Oncorhynchus tshawytscha and juvenile steelhead/rainbow trout O. mykiss from three populations in the Grande Ronde River basin. Based on migration timing and abundance, two distinct life-history strategies of juvenile spring chinook and O.mykiss could be distinguished. An early migrant group left upper rearing areas from July through January with a peak in the fall. A late migrant group descended from upper rearing areas from February through June with a peak in the spring.

157

Grain mantles: The impact on grain evolution and selective extinction

A variety of observations suggest that the Universe had a hot dense origin and that the pregalactic composition of the Universe was determined by nuclear reactions that occurred in the first few minutes. There is no unique hot Big Bang theory, but the simplest version produces a primeval chemical composition that is in good qualitative agreement with the abundances deduced from observation. Whether or not any Big Bang theory will provide quantitative agreement with observations depends on a variety of factors in elementary particle physics (number and masses of stable or long-lived particles, half-life of neutron, structure of grand unified theories) and from observational astronomy (present mean baryon density of the Universe, the Hubble constant and deceleration parameter). The influence of these factors on the abundances is discussed, as is the effect of departures from homogeneity and isotropy in the early Universe.

1982-10-15

158

Geological characteristics and resource potentials of oil shale in Ordos Basin, Center China

Depletion studies are used to infer the presence of mantles and to constrain grain evolutionary models in the diffuse interstellar medium. The presence of these mantles appears to be important in the evolution of the grains inside diffuse as well as dense clouds. In dense clouds where the element-to-element abundances sometimes differ from those found in diffuse clouds, empirical relationships are starting to emerge between gas abundances and various types of peculiar selective extinction. These peculiar extinction curves may be the results of nonvolatile mantle formation on grain cores or may reflect chemical differences due to variations in the intrinsic metalicity from one cloud to another. A simple model of the time evolution of a parcel of gas and dust as observed by the depletion of two elements is presented. Different studies of grain evolution and selective extinction are discussed and compared.

1989-12-01

159

Effects of indirect ionization on the charge state distributions observed with highly charged ion sources

It has been shown that not only there are abundant oil, gas, coal, coal-bed gas, groundwater and giant uranium deposits but also there are abundant oil shale resources in Ordos basin. It has been shown also that the thickness of oil shale is, usually, 4-36m, oil-bearing 1.5%-13.7%, caloric value 1.66-20.98MJ/kg. The resource amount of oil shale with burial depth less than 2000 m is over 2000x108t (334). Within it, confirmed reserve is about 1x108t (121). Not only huge economic benefit but also precious experience in developing oil shale may be obtained in Ordos basin.

2010-09-15

160

Do Thinning and Burning Sites Revegetated after Bauxite Mining Improve Habitat for Terrestrial Vertebrates?

Presently, most charge state distributions produced with highly charged ion sources are predicted with models that approximate the ionization process with the Lotz formula. The Lotz ionization cross sections decrease approximately geometrically with increasing charge state except for ions with very few vacancies, for ions with very few electrons, and for electron impact energies which barely exceed the ionization energy. The geometrical decrease causes these models to predict a maximum abundance for most of the charge states, which is only weakly dependent on the charge state. Experimental results, however, yield much higher abundances for ions with an empty M shell than ions with a partly filled M shell. This difference is explained with indirect ionization processes that are neglected by the Lotz approximation, and normally can be neglected for the ionization of the L shell, but can dominate the ionization of the M shell. (c) 2000 American ...

2000-02-01

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161

British Library Electronic Table of Contents (United Kingdom)

Thinning and burning forests established on revegetated mine pits in jarrah (Eucalyptus marginata) forests of south-west Australia is being considered as a management option to accelerate succession in sites with excessive tree densities. To assess the impact of thinning and burning on reptiles and small mammals, we installed trapping grids in eight thinned and burned sites, each paired with untreated controls. Of the eight pairs, four were in rehabilitated sites (planted with nonlocal species) and four were in restored sites (seeded with local species). Thinning and burning had no significant impact on the small mammal community, although Cercatetus concinnus was more abundant in rehabilitated sites. In contrast, thinning and burning significantly increased reptile abundance and species r...

2010-01-01

162

Direct nano ESI time-of-flight mass spectrometric investigations on lanthanide BTP complexes in the extraction-relevant diluent 1-octanol

Alpha-induced reactions in iridium

The present work focuses on investigations of a highly selective ligand for Am(III)/Ln(III) separation: bis-triazinyl-pyridine (BTP). By means of nano-electro-spray mass spectrometry, complex formation of BTP with selected elements of the lanthanide series is investigated. We show that the diluent drastically influences complex speciation. Measurements obtained in the extraction-relevant diluent 1-octanol show the occurrence of Ln(BTP){sub i} (i 1-3) species in different relative abundances, depending on the lanthanide used. Here, the relative abundances of the Ln(BTP){sub 3} complexes correlate with the distribution ratios for extraction to the organic phase of the respective lanthanide. (authors)

2009-12-15

163

Allelochemicals produced by Caribbean macroalgae and cyanobacteria have species-specific effects on reef coral microorganisms

The excitation function of ({alpha},{ital xn}) reactions on {sup 191}Ir (abundance 37.3%) and on {sup 193}Ir (abundance 62.7%) has been measured for the 17--55 MeV alpha-particle bombarding energy range. The stacked foil activation technique and {gamma}-ray spectroscopy were used to determine the cross sections. The experimental data were compared with calculated values obtained by means of a geometry-dependent hybrid model. The initial exciton number {ital n}{sub 0}=4 with {ital n}=2, {ital p}=2, and {ital h}=0 gives the best agreements with the presently measured results. To calculate the excitation function theoretically a computer code was used. This set of excitation functions provides a data basis for probing the validity of combined equilibrium and preequilibrium reaction models in a considerable energy range.

1992-05-01

164

British Library Electronic Table of Contents (United Kingdom)

Coral populations have precipitously declined on Caribbean reefs while algal abundance has increased, leading to enhanced competitive damage to corals, which likely is mediated by the potent allelochemicals produced by both macroalgae and benthic cyanobacteria. Allelochemicals may affect the composition and abundance of coral-associated microorganisms that control host responses and adaptations to environmental change, including susceptibility to bacterial diseases. Here, we demonstrate that extracts of six Caribbean macroalgae and two benthic cyanobacteria have both inhibitory and stimulatory effects on bacterial taxa cultured from the surfaces of Caribbean corals, macroalgae, and corals exposed to macroalgal extracts. The growth of 54 bacterial isolates was monitored in the presence of l...

2011-01-01

165

T-2 mycotoxin inhibits mitochondrial protein synthesis

Phosphorylation of proteins in Clostridium thermohydrosulfuricum

The authors investigated the effect of T-2 toxin on rat liver mitochondrial protein synthesis. Isolated rat liver mitochondria were supplemented with an S-100 supernatant from rat liver and an external ATP-generating system. An in-vitro assay employing cycloheximide, and inhibitor of cytoplasmic protein synthesis, and chloramphenicol, and inhibitor of mitochondrial protein synthesis, to distinguish mitochondrial protein synthesis from the cytoplasmic process. Amino acid incorporation into mitochondria was dependent on the concentration of mitochondria and was inhibited by chloramphenicol. The rate of uptake of tritium leucine into mitochondrial protein was unaffected by the addition of T-2 toxin and was not a rate-limiting step in incorporation. However, 0.02 micrograms/ml of T-2 toxin decreased the rate of protein synthesis inhibition correlated with the amount ...

1988-01-01

166

Affinity purification of sequence-specific DNA binding proteins.

Cell extracts of the thermophile Clostridium thermohydrosulfuricum catalyzed the phosphorylation by (..gamma..-/sup 32/P)ATP of several endogenous proteins with M/sub r/s between 13,000 and 100,000. Serine and tyrosine were the main acceptors. Distinct substrate proteins were found in the soluble (e.g., proteins p66, p63, and p53 of M/sub r/s 66,000, 63,000, and 53,000, respectively) and particulate (p76 and p30) fractions, both of which contained protein kinase and phosphatase activity. The soluble fraction suppressed the phosphorylation of particulate proteins and contained a protein kinase inhibitor. Phosphorylation of p53 was promoted by 10..mu..M fructose 1,6-bisphosphate or glucose 1,6-bisphosphate and suppressed by hexose monophosphates, whereas p30 and p13 were suppressed by 5 ..mu..M brain (but not spinach) calmodulin. Polyamines, including the odd ...

1986-02-01

167

The Sorcerer II Global Ocean Sampling Expedition: Expanding theUniverse of Protein Families

We describe a method for affinity purification of sequence-specific DNA binding proteins that is fast and effective. Complementary chemically synthesized oligodeoxynucleotides that contain a recognition site for a sequence-specific DNA binding protein are annealed and ligated to give oligomers. This DNA is then covalently coupled to Sepharose CL-2B with cyanogen bromide to yield the affinity resin. A partially purified protein fraction is combined with competitor DNA and subsequently passed through the DNA-Sepharose resin. The desired sequence-specific DNA binding protein is purified because it preferentially binds to the recognition sites in the affinity resin rather than to the nonspecific competitor DNA in solution. For example, a protein fraction that is enriched for transcription factor Sp1 can be further purified 500- to 1000-fold by two sequential affinity chromatography ...

1986-08-01

168

Uniform and Residue-specific {sup 15}N-labeling of Proteins on a Highly Deuterated Background

Metagenomics projects based on shotgun sequencing of populations of micro-organisms yield insight into protein families. We used sequence similarity clustering to explore proteins with a comprehensive dataset consisting of sequences from available databases together with 6.12 million proteins predicted from an assembly of 7.7 million Global Ocean Sampling (GOS) sequences. The GOS dataset covers nearly all known prokaryotic protein families. A total of 3,995 medium- and large-sized clusters consisting of only GOS sequences are identified, out of which 1,700 have no detectable homology to known families. The GOS-only clusters contain a higher than expected proportion of sequences of viral origin, thus reflecting a poor sampling of viral diversity until now. Protein domain distributions in the GOS dataset and current protein databases show distinct biases. Several ...

2006-03-23

169

Proteomic analysis of sericin in Bombyx mori cocoons

A general method for stable-isotope labeling of large proteins is introduced and applied for studies of the E. coli GroE chaperone proteins by solution NMR. In addition to enabling the residue-specific {sup 15}N-labeling of proteins on a highly deuterated background, it is also an efficient approach for uniform labeling. The method meets the requirements of high-level deuteration, minimal cross-labeling and high protein yield, which are crucial for NMR studies of structures with sizes above 150 kDa. The results obtained with the new protocol are compared to other strategies for protein labeling, and evaluated with regard to the influence of external factors on the resulting isotope labeling patterns. Applications with the GroE system show that these strategies are efficient tools for studies of structure, dynamics and intermolecular interactions in large supramolecular complexes, ...

2004-07-15

170

British Library Electronic Table of Contents (United Kingdom)

Cocoon sericin plays an important role in the reeling of silk and serves as a valuable biomaterial in the field of biomedicine, skincare, and food industries; however, knowledge about cocoon sericin proteins has been limited. For a comprehensive study on sericin, cocoons of eight varieties of silkworm of different geographic origin and with varied cocoon color were analyzed utilizing proteomics and bioinformatics approaches. The electrophoresis pattern demonstrated some common protein bands for all silkworm varieties and distinctive protein bands for some of those examined in the present study. The Ser2 protein, a new Ser3 protein, and four other novel sericin proteins were identified in cocoons for the first time. Products of both Ser1 and Ser3 genes appear to be ubiquitous in the cocoon ...

2011-01-01

171

Modeling protein synthesis from a physicist's perspective: a toy model

Mechanism of Dephosphorylation of the SR Protein ASF/SF2 by Protein Phosphatase 1

Proteins are polymers of amino acids. These macromolecules are synthesized by intracellular machines called {\\it ribosome}. Although, traditionally, the experimental investigation of protein synthesis has been an active area of research in molecular cell biology, important quantitative models of this phenomenon have been reported mostly in the research journals devoted to statistical physics and related interdisciplinary topics. From the perspective of a physicist, protein synthesis is a phenomenon of {\\it classical transport of interacting ribosomes on a messenger RNA (mRNA) template} that dictates the sequence of the amino acids on the protein. Here we bring this frontier area of contemporary research into the classroom by appropriate simplification of the models and methods. In particular, we develope a simple toy model and analyze it by some elementary techniques of non-equilibrium statistical ...

2007-01-01

172

British Library Electronic Table of Contents (United Kingdom)

SR proteins are essential splicing factors whose function is controlled by multi-site phosphorylation of a C-terminal domain rich in arginine-serine repeats (RS domain). The protein kinase SRPK1 has been shown to polyphosphorylate the N-terminal portion of the RS domain (RS1) of the SR protein ASF/SF2, a modification that promotes nuclear entry of this splicing factor and engagement in splicing function. Later, dephosphorylation is required for maturation of the spliceosome and other RNA processing steps. While phosphates are attached to RS1 in a sequential manner by SRPK1, little is known about how they are removed. To investigate factors that control dephosphorylation, we monitored region-specific mapping of phosphorylation sites in ASF/SF2 as a function of the protein phosphatase PP1. W...

2010-01-01

173

Growth Inhibition and Induction of Stress Protein, GroEL, of Bacillus cereus Exposed to Antibacterial Peptide Isolated from Bacillus subtilis SC-8

British Library Electronic Table of Contents (United Kingdom)

This study was conducted to investigate the antibacterial effect of BSAP-254 on Bacillus cereus with the induced stress proteins. The BSAP-254 is an antimicrobial peptide isolated from soybean-fermenting bacteria, Bacillus subtilis SC-8. It had a narrow spectrum of activity against B. cereus group. The growth inhibitory effect of BSAP-254 (50??g/mL) reduced the population of B. cereus from >108 to 104 colony-forming units per milliliter within 30?min. In B. cereus exposed to BSAP-254, 14 intracellular proteins were differentially expressed as determined by 2-DE coupled with MS. Of the differentially expressed proteins identified, the stress protein GroEL, which is heat shock protein, was induced in B. cereus exposed to antibacterial peptide.

2011-01-01

174

The ecological role of bacteriocins in bacterial competition.

Scientific Assessment of Ozone Depletion: 2002. Pursuant to Article 6 of the Montreal Protocol on Substances that Deplete the Ozone Layer

Bacteriocins are an abundant class of antimicrobial molecules that appear to mediate population dynamics within species. The bacteriocins of Escherichia coli have served as a model for exploring the ecological role of these potent toxins. Studies suggest that colicins provide a competitive edge in nutrient-poor environments and that there might be a trade-off between the costs and benefits of colicin production. PMID:10203843

1999-03-01

175

Review of Panel on Reference Nuclear Data surveys and discussions on reference nuclear data needs

Since the Scientific Assessment of Ozone Depletion: 1998, numerous laboratory investigations, atmospheric observations, and theoretical and modeling studies have produced new key findings and have strengthened overall understanding of the ozone layer and its effect on ultraviolet (UV) radiation. These advances are highlighted in the following summary of the current understanding of the impact of human activities and natural phenomena on the ozone layer and the coupling of the ozone layer and the climate system. In the troposphere (i.e., lower atmosphere), observations show that the total combined effective abundance of ozone-depleting compounds continues to decline slowly from the peak that occurred in 1992-1994. Total chlorine is declining, while bromine from industrial halons is still increasing, albeit at a slower rate than was occurring previously (and as reported in the 1998 Assessment). Analyses of air trapped in snow since the late 19th century have ...

2003-01-01

176

Presentation of twentieth century atmospheric carbon dioxide record in Smithsonian spectrographic plates

The results of the six meetings in the years 1976 through 1981 of the Panel on Reference Nuclear Data is reviewed, as well as the results of other studies and workshops. A brief review of how current programs are meeting the expressed nuclear data needs of professional groups is also presented. The types of data included in the review are reaction cross sections, isotopic abundances, atomic masses, nuclear structure, radioactive decay, and fission.

1982-10-01

177

Phthalate esters used as plasticizers in packed red blood cell storage bags may lead to progressive toxin exposure and the release of pro-inflammatory cytokines

The observed strength of the carbon dioxide absorption bands recorded on spectrobolograms of the transparency of the earth's atmosphere between wavelengths 300 and 2500 nanometers as part of the Smithsonian Solar Constant Program were used to extract the abundance of carbon dioxide in the atmosphere. The details of the analysis and the sources of error are discussed. 11 references, 5 figures, 1 table. (ACR)

1983-11-01

178

Nucleosynthesis in the Hot Convective Bubble in Core-Collapse Supernovae

Phthalate esters (PE's) are plasticizers used to soften PVC-based medical devices. PE's are the most abundant man-made pollutants and increase the risk of developing an allergic respiratory disease...Full Text Available

2009-07-01

179

Harmful Algae, Nuisance Blooms and Anthropogenic Nutrient Enrichment

As an explosion develops in the collapsed core of a massive star, neutrino emission drives convection in a hot bubble of radiation, nucleons, and pairs just outside a proto-neutron star. Shortly thereafter, neutrinos drive a wind-like outflow from the neutron star. In both the convective bubble and the early wind, weak interactions temporarily cause a proton excess (Y{sub e} {approx}> 0.50) to develop in the ejected matter. This situation lasts for at least the first second, and the approximately 0.05-0.1 M{sub {circle_dot}} that is ejected has an unusual composition that may be important for nucleosynthesis. Using tracer particles to follow the conditions in a two-dimensional model of a successful supernova explosion calculated by Janka, Buras, and Rampp (2003), they determine the composition of this material. most of it is helium and {sup 56}Ni. The rest is relatively rare species produced by the decay of proton-rich isotopes unstable to positron emission. In the absence of ...

2004-09-02

180

Environmental Research Database

DescriptionPhytoplankton is the collective name given to the microscopic floating plants in seas and lakes. Under certain conditions, the abundance of phytoplankton as a whole or of one or more species in particular, can reach a magnitude at which it is visible through discolouration of the sea. Some of these blooms because of the colour of the water have been called 'Red Tides'. Blooms of some 300 species of the phytoplankton are known as Harmful Algal Bloom (HAB) species in recognition of their poten [continued...

2009-01-31

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181

Dynamical evolution and molecular abundances of interstellar clouds

Detection and Isolation of Ultrasmall Microorganisms from a 120,000-Year-Old Greenland Glacier Ice Core

Dynamical models are presented that start with interstellar gas in an initial diffuse state and consider their gravitational collapse and the formation of dense cores. Frozen-in tangled magnetic fields are included to mimic forces that might oppose gravitational contraction and whose effectiveness may increase with increasing core densities. Results suggest the possibility that dense cloud cores may be dynamically evolving ephemeral objects, such that their lifespan at a given core density decreases as that density increases. 66 refs.

182

Cross sections and excitation functions of 40 to 110 MeV #alpha#-particle induced reactions in natural chromium

The abundant microbial population in a 3,043-m-deep Greenland glacier ice core was dominated by ultrasmall cells (<0.1 μm³) that may represent intrinsically small organisms...Full Text Available

2005-12-01

183

Concentration and Preservation of Very Low Abundance Biomarkers in Urine, such as Human Growth Hormone (hGH), by Cibacron Blue F3G-A Loaded Hydrogel Particles

The reactions of alpha particles on natural abundance chromium have been studied between 40 and 110 MeV, in order to produce "5"2Fe for medical use. The yield of the reaction "5"2Cr(#alpha#,4n)"5"2Fe for Esub(#alpha#) = 85 #-># 53 MeV is only 30 #mu#Ci/#mu#Ah. (orig.).

184

Big bang nucleosynthesis and finite temperature field theory

Urine is a potential source of diagnostic biomarkers for detection of diseases, and is a very attractive means of non-invasive biospecimen collection. Nonetheless, proteomic measurement in urine...Full Text Available

2008-12-01

185

Ambient water-quality criteria for ammonia (salt water)-1989

We consider electromagnetic corrections at finite temperature and their effect on the nucleosynthesis in the standard Big Bang scenario. This requires discussing the finite, temperature dependent correction to the neutron-proton mass difference as well as making use of a previous result on the temperature correction to the mass of the electron. We find that these corrections do not affect the conventional results of e.g. the helium abundance to any appreciable extent.

1982-11-11

186

Abundances of s-process elements in planetary nebulae: Br, Kr & Xe

Ammonia is a common and highly toxic pollutant which, in sufficient quantities, will adversely affect aquatic organisms. This ammonia criteria document for salt water will allow establishment of regulatory standards for ammonia discharge into estuaries, near coastal zones and oceans. Possible adverse impacts in highly sensitive and abundant ecosystems such as estuaries make regulation particularly important.

1989-04-01

187

Abundance of Alnus incana ssp. rugosa in Adirondack Mountain shrub wetlands and its influence on inorganic nitrogen

We identify emission lines of post-iron peak elements in very high signal-to-noise spectra of a sample of planetary nebulae. Analysis of lines from ions of Kr and Xe reveals enhancements in most of the PNe, in agreement with the theories of s-process in AGB star. Surprisingly, we did not detect lines from Br even though s-process calculations indicate that it should be produced with Kr at detectable levels.

2006-01-01

188

Wiskott-Aldrich syndrome protein is required for regulatory T cell homeostasis

The presence of the nitrogen-fixing shrub, Alnus incana ssp. rugosa, influences the concentration of inorganic nitrogen in surface waters in the Adirondack Mountain region of northern New York. - The purpose of this research was to determine the abundance of the nitrogen-fixing shrub, Alnus incana ssp. rugosa (speckled alder), in shrub wetlands of the Adirondack Mountain region of New York State and to determine whether its abundance affects the concentration or accumulation of inorganic nitrogen in wetland substrates. Alder/willow wetlands are the second most common wetland type in the Adirondack region. The Adirondack Park Agency's digital GIS database of wetland types was used to determine the areal extent of alder/willow wetlands in the Adirondacks. Randomly selected wetlands were sampled to determine the size and abundance of alder. Alder densities averaged {approx}7000 stems ha{sup -1} and alder was present ...

2003-06-01

189

Werner syndrome protein interacts functionally with translesion DNA polymerases

Wiskott-Aldrich syndrome protein (WASp) is essential for optimal T cell activation. Patients with WAS exhibit both immunodeficiency and a marked susceptibility to systemic autoimmunity. We investigated...Full Text Available

2007-02-01

190

Water Molecule Contributions to Proton Spin-Lattice Relaxation in Rotationally Immobilized Proteins

Werner syndrome (WS) is characterized by premature onset of age-associated disorders and predisposition to cancer. The WS protein, WRN, encodes 3′ → 5′ DNA helicase and 3′...Full Text Available

2007-06-19

191

Variable domain I of nematode CLEs directs post-translational targeting of CLE peptides to the extracellular space

Spin-lattice relaxation rates of protein and water protons in dry and hydrated immobilized bovine serum albumin were measured in the range of ¹H Larmor frequency from 10 kHz to 30...Full Text Available

2009-07-01

192

Use of synthetic oligoribonucleotides to probe RNA-protein interactions in the MS2 translational operator complex.

Effector proteins expressed in the esophageal gland cells of cyst nematodes are delivered into plant cells through a hollow, protrusible stylet. Although evidence indicates that effector proteins function...Full Text Available

2010-12-01

193

Use of Dimethyl Suberimidate, a Cross-Linking Reagent, in Studying the Subunit Structure of Oligomeric Proteins^*

Synthetic oligoribonucleotides have been used to probe the interaction of MS2 coat protein with the translational operator of the MS2 replicase gene. We have investigated the possible formation of a...Full Text Available

1990-06-25

194

Use of DNA ladders for reproducible protein fractionation by SDS-PAGE for quantitative proteomics

Amidination of aldolase, glyceraldehyde-3-phosphate dehydrogenase, tryptophan synthetase B protein, L-arabinose isomerase, and the catalytic subunit of E. coli aspartate transcarbamylase...Full Text Available

1970-07-01

195

The requirement of yeast replication origins for pre-replication complex proteins is modulated by transcription

In proteomics, one-dimensional (1D) SDS-PAGE is widely used for protein fractionation prior to mass spectrometric analysis to enhance dynamic range of analysis and to improve identification...Full Text Available

2008-02-01

196

The region encompassing the procyclic acidic repetitive protein (PARP) gene promoter plays a role in plasmid DNA replication in Trypanosoma brucei.

The mini-chromosome maintenance proteins Mcm2–7 are essential for DNA replication. They are loaded onto replication origins during G1 phase of the cell cycle to form a pre-replication complex...Full Text Available

2005-01-01

197

The long lifespan of two bat species is correlated with resistance to protein oxidation and enhanced protein homeostasis

We have previously reported the construction and characterization of an autonomously replicating plasmid in Trypanosoma brucei. In this plasmid the procyclic acidic repetitive protein (PARP) gene promoter...Full Text Available

1994-10-11

198

The discovery of signal transduction by G proteins. A personal account and an overview of the initial findings and contributions that led to our present understanding

Altered structure, and hence function, of cellular macromolecules caused by oxidation can contribute to loss of physiological function with age. Here, we tested whether the lifespan of bats, which generally...Full Text Available

2009-07-01

199

The Effects of Aspirin-Like Drugs on the Nutritional Status of Pregnant Rats and Offspring

The realization that there existed a G-protein coupled signal transduction mechanism developed gradually and was initially the result of an ill fated quest for uncovering the mechanism of action...Full Text Available

2007-04-01

200

Synthesis and Phosphorylation of Maize Acidic Ribosomal Proteins1

Previous studies have shown that salicylates and protein-calorie malnutrition independently compromise maturation and growth of infants. In the present study, pregnant rats were fed normal-and low-protein...Full Text Available

1988-05-01

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201

Surfactant protein A, an innate immune factor, is expressed in the vaginal mucosa and is present in vaginal lavage fluid

The objective of this research was to determine the role of acidic ribosomal protein (ARP) phosphorylation in translation. Ribosomes (Rbs) from germinated maize (Zea mays L.) axes had...Full Text Available

1998-01-01

202

Stimulation of Phosphatidylethanolamine Exchange by Castor Bean Cytosol Proteins ¹

Surfactant protein A (SP-A), first identified as a component of the lung surfactant system, is now recognized to be an important contributor to host defence mechanisms. SP-A can facilitate phagocytosis...Full Text Available

2004-01-01

203

Simulation of the electron-paramagnetic-resonance spectrum of the iron-protein of nitrogenase. A prediction of the existence of a second paramagnetic centre.

Cytosol proteins prepared from castor bean endosperm (4-day-old) seedlings stimulate the exchange of [³H]phosphatidylethanolamine between liposomes and mitochondria. The acceleration of the...Full Text Available

1980-02-01

204

Seroprevalence of HIV-1 and HIV-2 infection among children diagnosed with protein-calorie malnutrition in Nigeria.

The e.p.r. spectra of the Fe-proteins of nitrogenase from all sources studied have unusual features in that they have very anisotropic linewidths and low integrated intensities. These characteristics...Full Text Available

1978-12-01

205

Role in Virulence of a Brucella abortus Protein Exhibiting Lectin-Like Activity

Excessive weight loss due to protein calorie malnutrition (PCM) is a significant problem in Nigerian children. This syndrome may be difficult to differentiate from the wasting disease caused by human...Full Text Available

1993-04-01

206

Risk factors for thrombosis in lupus patients.

Brucella abortus is a facultative, intracellular zoonotic pathogen which can cause undulant fever in humans and abortions in cattle. A 14-kDa protein of B. abortus...Full Text Available

2006-01-01

207

Reinvestigation of the effect of carbenoxolone on the induction of heat shock proteins

Lupus anticoagulant, concentrations of anticardiolipin antibodies, antithrombin III, plasminogen, (free) protein S, protein C, prothrombin, platelet counts, and bleeding times were determined in 74...Full Text Available

1989-11-01

208

Proteomic approach with LCMS-IT-TOF identified an increase of Rab33B after transient focal cerebral ischemia in mice

Carbenoxolone (CBX) is a semisynthetic derivative of the licorice root substance glycyrrhizinic acid and has been previously reported to induce only heat shock protein 70 [Hsp70, HSPA1A (the systematic...Full Text Available

2009-09-01

209

Predicting Protein Post-translational Modifications Using Meta-analysis of Proteome Scale Data Sets*S?

BackgroundSeveral proteins are known to be markedly expressed in the brain during cerebral ischemia; however, the changes in protein profiles within the ischemic brain after an ischemic...Full Text Available

210

Plasma protein binding of phenytoin in 100 epileptic patients.

Protein post-translational modifications are an important biological regulatory mechanism, and the rate of their discovery using high throughput techniques is rapidly increasingly. To make use of this...Full Text Available

2009-02-01

211

Photophysics and optical switching in green fluorescent protein mutants

The plasma protein binding of phenytoin was investigated in 100 epileptic patients, using equilibrium dialysis at 37 degrees C. The unbound fractions of phenytoin in plasma formed a skewed distribution,...Full Text Available

1982-08-01

212

Pharmacokinetics and protein binding of ceftriaxone during pregnancy.

We demonstrate by using low-temperature high-resolution spectroscopy that red-shifted mutants of green fluorescent protein are photo-interconverted among three conformations and are, therefore, not...Full Text Available

2000-03-28

213

Overexpression of human virus surface glycoprotein precursors induces cytosolic unfolded protein response in Saccharomyces cerevisiae

The purpose of the present work was to study the pharmacokinetics and the protein binding (free fraction of the drug) of ceftriaxone (CTX) during pregnancy. Nine pregnant women (ages, 20 to 34 years)...Full Text Available

1993-01-01

214

Novel structural features in two ZHX homeodomains derived from a systematic study of single and multiple domains

BackgroundThe expression of human virus surface proteins, as well as other mammalian glycoproteins, is much more efficient in cells of higher eukaryotes rather than yeasts. The limitations...Full Text Available

215

Multiple-Bond Kinetics from Single-Molecule Pulling Experiments: Evidence for Multiple NCAM Bonds

BackgroundZhx1 to 3 (zinc-fingers and homeoboxes) form a set of paralogous genes encoding multi-domain proteins. ZHX proteins consist of two zinc fingers followed...Full Text Available

216

Molecular Identification and Expression Analysis of Filaggrin-2, a Member of the S100 Fused-Type Protein Family

The kinetic parameters of single bonds between neural cell adhesion molecules were determined from atomic force microscope measurements of the forced dissociation of the homophilic protein-protein bonds....Full Text Available

2005-11-01

217

Molecular Architecture of the Centriole Proteome: The Conserved WD40 Domain Protein POC1 Is Required for Centriole Duplication and Length Control

Genes of the S100 fused-type protein (SFTP) family are clustered within the epidermal differentiation complex and encode essential components that maintain epithelial homeostasis and barrier functions....Full Text Available

218

Metal Ions-Stimulated Iron Oxidation in Hydroxylases Facilitates Stabilization of HIF-1? Protein

Centrioles are intriguing cylindrical organelles composed of triplet microtubules. Proteomic data suggest that a large number of proteins besides tubulin are necessary for the formation and maintenance...Full Text Available

2009-02-15

219

Membrane protein biosensing with plasmonic nanopore arrays and pore-spanning lipid membranes

The exposure of cells to several metal ions stabilizes HIF-1α protein. However, the molecular mechanisms are not completely understood. They may involve inhibition of hydroxylation by either...Full Text Available

2009-02-01

220

Measurements of accurate x-ray scattering data of protein solutions using small stationary sample cells

Integration of solid-state biosensors and lipid bilayer membranes is important for membrane protein research and drug discovery. In these sensors, it is critical that the solid-state sensing...Full Text Available

2010-01-01

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221

MFR, a Putative Receptor Mediating the Fusion of Macrophages

In this paper, we report a method of precise in situ x-ray scattering measurements on protein solutions using small stationary sample cells. Although reduction in the radiation damage...Full Text Available

2009-01-01

222

M protein (M1) of influenza virus: antigenic analysis and intracellular localization with monoclonal antibodies.

We had previously identified a macrophage surface protein whose expression is highly induced, transient, and specific, as it is restricted to actively fusing macrophages in vitro and in vivo. This protein...Full Text Available

1998-11-01

223

Los Alamos sequence analysis package for nucleic acids and proteins.

A panel of 16 monoclonal antibodies recognizing M protein (M1) of influenza virus was generated. Competition analyses resulted in localization of 14 monoclonal antibodies to three antigenic sites. Three...Full Text Available

1989-09-01

224

Lipoprotein lipase deficiency is associated with elevated acylation stimulating protein plasma levels

An interactive system for computer analysis of nucleic acid and protein sequences has been developed for the Los Alamos DNA Sequence Database. It provides a convenient way to search or verify various...Full Text Available

1982-01-11

225

Linking Gene Expression and Functional Network Data in Human Heart Failure

Acylation stimulating protein (ASP, C3adesArg) is an adipose tissue derived hormone that stimulates triglyceride (TG) synthesis. ASP stimulates lipoprotein lipase (LPL) activity by relieving feedback...Full Text Available

2009-06-01

226

Lifespan extension by increased expression of the Drosophila homologue of the IGFBP7 tumour suppressor

BackgroundGene expression profiling and the analysis of protein-protein interaction (PPI) networks may support the identification of disease bio-markers and potential drug targets....Full Text Available

227

Lectin Binding of the Major Polar Tube Protein (PTP1) and its Role in Invasion

Mammals possess multiple insulin-like growth factor (IGF) binding proteins (IGFBPs), and related proteins, that modulate the activity of insulin/IGF signalling (IIS), a conserved neuroendocrine signalling...Full Text Available

2011-02-01

228

Lack of association between polymorphisms in C4b-binding protein and atypical haemolytic uraemic syndrome in the Spanish population

... 2003)050[0600:LBOTMP]2.0.CO;2 Lectin Binding of the Major Polar Tube Protein (PTP1) ... PTP1. We, therefore, undertook a study ...

229

Isolation and Characterization of a Novel Nuclear Protein from Pollen Mother Cells of Lily

Dysregulation of the alternative pathway of complement activation, caused by mutations or polymorphisms in the genes encoding factor H, membrane co-factor protein, factor I or factor B, is associated...Full Text Available

2009-01-01

230

Involvement of AMP-activated-protein-kinase (AMPK) in neuronal amyloidogenesis

Pollen mother cells of the lily (Lilium speciosum) were found to have a histone-H1-like protein (PMCP) not detected in other tissues. The PMCP appears from the late S-G₂...Full Text Available

1990-11-01

231

Investigation of Early Protein Changes in the Urinary Bladder Following Partial Bladder Outlet Obstruction by Proteomic Approach

AMP-activated-protein-kinase (AMPK) is a key sensor and regulator of cellular and whole-body energy metabolism and plays a key role in regulation of lipid metabolism. Since lipid metabolism...Full Text Available

2010-09-03

232

Intracellular delivery of an antisense?oligonucleotide via endocytosis of a G protein-coupled receptor

We investigated the pathophysiological mechanism by proteomic approach as a possible tool to detect the marker proteins to develop lower urinary tract symptoms following bladder outlet obstruction (BOO)....Full Text Available

2005-12-01

233

Interaction between Bluetongue virus outer capsid protein VP2 and vimentin is necessary for virus egress

Gastrin-releasing peptide receptor (GRPR), a member of the G protein-coupled receptor superfamily, has been utilized for receptor-mediated targeting of imaging and therapeutic agents; here we extend...Full Text Available

2010-10-01

234

Insulin-induced decrease in protein phosphorylation in rat adipocytes not explained by decreased A-kinase activity

BackgroundThe VP2 outer capsid protein Bluetongue Virus (BTV) is responsible for receptor binding, haemagglutination and eliciting host-specific immunity. However, the assembly of...Full Text Available

235

Insertion in the mRNA of a metachromatic leukodystrophy patient with sphingolipid activator protein-1 deficiency.

In isolated rat adipocytes, insulin inhibits lipolysis to a greater extent than would be predicted by the decrease in (-/+)cAMP activity ratio of cAMP-dependent protein kinase (A-kinase), from which it was speculated that insulin promotes the dephosphorylation of hormone-sensitive lipase. They have examined the phosphorylation state of cellular proteins under conditions of varying A-kinase activities in the presence and absence of insulin. Protein phosphorylation was determined by SDS-PAGE electrophoresis of extracts from /sup 32/P-loaded cells; glycerol and A-kinase activity ratios were measured in the cytosolic extracts from control, non-radioactive cells. Increased protein phosphorylation in general occurred over the same range of A-kinase activity ratios, 0.1-0.3, associated with increased glycerol release. The insulin-induced decrease in lipolysis was associated with a decrease in the /sup 32/P ...

1987-05-01

236

Inferring Stabilizing Mutations from Protein Phylogenies: Application to Influenza Hemagglutinin

The lysosomal catabolism of sulfatide requires arylsulfatase A and a specific sphingolipid activator protein, SAP-1. While most patients with metachromatic leukodystrophy have mutations in the gene...Full Text Available

1990-02-01

237

Individual Mycobacterium tuberculosis universal stress protein homologues are dispensable in vitro

One selection pressure shaping sequence evolution is the requirement that a protein fold with sufficient stability to perform its biological functions. We present...Full Text Available

2009-04-01

238

In vitro protein binding of cefonicid and cefuroxime in adult and neonatal sera.

SummaryMycobacterium tuberculosis has 10 universal stress proteins, whose function is unknown. However, proteomic and transcriptomic analyses have shown that a number...Full Text Available

2010-07-01

239

Identification of Host Proteins Associated with Retroviral Vector Particles by Proteomic Analysis of Highly Purified Vector Preparations?

The levels of in vitro protein binding of cefonicid and cefuroxime in human adult and neonatal sera were compared. Binding parameters for each drug were determined within the concentration range of...Full Text Available

1993-06-01

240

How Protein Stability and New Functions Trade Off

The Moloney murine leukemia virus (MMLV) belongs to the Retroviridae family of enveloped viruses, which is known to acquire minute amounts of host cellular proteins both on the surface...Full Text Available

2008-02-01

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241

Functional properties of membrane cofactor protein of complement.

Numerous studies have noted that the evolution of new enzymatic specificities is accompanied by loss of the protein's thermodynamic stability (ΔΔG), thus suggesting...Full Text Available

2008-02-01

242

Fatty Acid- and Retinoid-binding Proteins Have Distinct Binding Pockets for the Two Types of Cargo*

Membrane cofactor protein (MCP or gp45-70) of the complement system is a cofactor for factor I-mediated cleavage of fluid-phase C3b and C3b-like C3, which opens the thioester bond. In the present study...Full Text Available

1989-12-01

243

Evidence for nonrandom hydrophobicity structures in protein chains.

Parasitic nematodes cause serious diseases in humans, animals, and plants. They have limited lipid metabolism and are reliant on lipid-binding proteins to acquire these metabolites from their hosts....Full Text Available

2009-12-18

244

Evaluation of heavy-metal ion toxicity in fish cells using a combined stress protein and cytotoxicity assay

The question of whether proteins originate from random sequences of amino acids is addressed. A statistical analysis is performed in terms of blocked and random walk values formed by binary hydrophobic...Full Text Available

1996-09-03

245

Evaluation of Two Homologous Proline-Rich Proteins of Coccidioides posadasii as Candidate Vaccines against Coccidioidomycosis?

All organisms, from bacteria and yeast to humans, respond to physical and chemical stressors by increasing the synthesis of a small group of cellular stress proteins.'' The authors have developed a simple in vitro system for quickly screening environmentally relevant stressors to detect stress-induced proteins that are good candidates for biomarkers. Polyacrylamide gel electrophoresis was used to detect stressor-induced, concentration-dependent changes in cellular stress protein levels in two fish cell culture systems, whereas simultaneous in vitro neutral red uptake cytotoxicity assays measured the stressors effect on cellular physiology. There was a direct concentration-dependent relationship between sublethal cytotoxic effects and the increases in stress protein levels. Increases of 50 to 200% were detected in stress proteins from desert topminnow, Poeciliopsis ...

1994-08-01

246

EhMAPK, the Mitogen-Activated Protein Kinase from Entamoeba histolytica Is Associated with Cell Survival

Evaluation of the protective efficacy of recombinant T-cell-reactive proteins of Coccidioides posadasii in a murine model of coccidioidomycosis has led to the discovery of potential...Full Text Available

2007-12-01

247

Effects of PKA phosphorylation on the conformation of the Na,K-ATPase regulatory protein FXYD1

Mitogen Activated Protein Kinases (MAPKs) are a class of serine/threonine kinases that regulate a number of different cellular activities including cell proliferation, differentiation, survival and...Full Text Available

248

Effects of Essential Oils on Ruminal Microorganisms and Their Protein Metabolism

FXYD1 (phospholemman) is a member of an evolutionarily conserved family of membrane proteins that regulate the function of the Na,K-ATPase enzyme complex in specific tissues and specific physiological...Full Text Available

2009-11-01

249

E2f binding-deficient Rb1 protein suppresses prostate tumor progression in vivo

A commercial blend of essential oil (EO) compounds was added to a grass, maize silage, and concentrate diet fed to dairy cattle in order to determine their influence on protein metabolism by ruminal...Full Text Available

2003-08-01

250

Display of Recombinant Proteins on Bacillus subtilis Spores, Using a Coat-Associated Enzyme as the Carrier?

Mutational inactivation of the RB1 tumor suppressor gene initiates retinoblastoma and other human cancers. RB1 protein (pRb) restrains cell proliferation by binding...Full Text Available

2011-01-11

251

Diet-induced changes in uncoupling proteins in obesity-prone and obesity-resistant strains of mice

The display of proteins such as feed enzymes at the surface of bacterial spore systems has a great potential use for animal feed. Feed enzymes increase the digestibility of nutrients, leading to greater...Full Text Available

2010-09-01

252

Determinants of the plasma protein binding of theophylline in health.

Uncoupling protein 2 (UCP2) maps to a region on distal mouse chromosome 7 that has been linked to the phenotypes of obesity and type II diabetes. We recently reported that UCP2 expression is increased...Full Text Available

1998-03-31

253

Dengue virus protein recognition by virus-specific murine CD8+ cytotoxic T lymphocytes.

1 The plasma protein binding of theophylline was determined after addition of [14C]-theophylline (15 micrograms/ml) to plasma from 24 healthy drug-free volunteers and equilibrium dialysis for 2 h at...Full Text Available

1983-04-01

254

Deletion of the Plasmodium falciparum Merozoite Surface Protein 7 Gene Impairs Parasite Invasion of Erythrocytes?

The identification of the protein targets for dengue virus-specific T lymphocytes may be useful for planning the development of subunit vaccines against dengue. We studied the recognition by murine...Full Text Available

1993-02-01

255

Degradation of Proteins Artificially Introduced into Vacuoles of Chara australis¹

Merozoite surface proteins have been implicated in the initial attachment to the host red blood cell membrane that begins the process of invasion, an important step in the life cycle of the malaria...Full Text Available

2008-12-01

256

Deglutathionylation of 2-Cys Peroxiredoxin Is Specifically Catalyzed by Sulfiredoxin*

When an exogenous protein, bovine serum albumin, was introduced into the vacuole of a Chara australis internodal cell, it was degraded with time. This degradation proceeded only in...Full Text Available

1988-12-01

257

Deficiency of circadian protein CLOCK reduces lifespan and increases age-related cataract development in mice

Reversible protein glutathionylation plays a key role in cellular regulation and cell signaling and protects protein thiols from hyperoxidation. Sulfiredoxin (Srx), an enzyme that catalyzes the reduction...Full Text Available

2009-08-28

258

DNA replication in Escherichia coli mutants that lack protein HU.

Circadian clock is implicated in the regulation of aging. The transcription factor CLOCK, a core component of the circadian system, operates in complex with another circadian clock protein BMAL1. Recently...Full Text Available

259

Cyclic Diguanylate Signaling Proteins Control Intracellular Growth of Legionella pneumophila

DNA replication in Escherichia coli cells lacking protein HU was studied. HU has been suggested to be involved in the initiation of replication from in vitro studies. The isolated HU mutants, however,...Full Text Available

1989-10-01

260

Coupling of Pressure-Induced Structural Shifts to Spectral Changes in a Yellow Fluorescent Protein

Proteins that metabolize or bind the nucleotide second messenger cyclic diguanylate regulate a wide variety of important processes in bacteria. These processes include motility, biofilm formation, cell...Full Text Available

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

261

Controlling Gas-Phase Reactions for Efficient Charge Reduction Electrospray Mass Spectrometry of Intact Proteins

AbstractX-ray diffraction analysis of pressure-induced structural changes in the Aequorea yellow fluorescent protein Citrine reveals the structural basis for the continuous...Full Text Available

2009-09-16

262

Computer-based analysis of Haemophilus parasuis protein fingerprints

Charge reduction electrospray mass spectrometry (CREMS) reduces the charge states of electrospray-generated ions, which concentrates the ions from a protein into fewer peaks spread over a larger...Full Text Available

2005-11-01

263

Computer simulation of protein self-association during small-zone gel filtration. Estimation of equilibrium constants.

AbstractThe present study aimed to compare the whole-cell protein profiles of Haemophilus parasuis field isolates by using a computer-based analysis, and evaluate the...Full Text Available

2004-01-01

264

Complement Factor H-Related Proteins CFHR2 and CFHR5 Represent Novel Ligands for the Infection-Associated CRASP Proteins of Borrelia burgdorferi

A simulation is developed that qualitatively describes the small-zone-gel-filtration behaviour of a reversibly associating protein. The results reflect the dependence of the apparent molecular weight...Full Text Available

1981-04-01

265

Clinical significance of Fas and FasL protein expression in gastric carcinoma and local lymph node tissues

BackgroundOne virulence property of Borrelia burgdorferi is its resistance to innate immunity, in particular to complement-mediated killing. Serum-resistant B....Full Text Available

266

Cleavage of sterol regulatory element binding proteins (SREBPs) by CPP32 during apoptosis.

AIM: To investigate the relation of Fas and Fas ligand (FasL) protein expression with carcinogenesis and metastasis of gastric carcinoma.METHODS: Immunohistochemistry was used to detect Fas and...Full Text Available

2010-03-14

267

Circulating antibody to myelin basic protein in relapsing-remitting multiple sclerosis? A comparative group and sequential study by radioimmunoassay.

Cellular cholesterol homeostasis is controlled by sterol-regulated proteolysis of membrane-bound transcription factors called sterol-regulatory element binding proteins (SREBPs). CPP32, a cysteine protease,...Full Text Available

1996-03-01

268

Characterization of group A streptococcal T-12 protein purified by ion-exchange column chromatography.

Sera from multiple sclerosis patients with relapsing-remitting disease and normal subjects were tested for antibody to myelin basic protein by a sensitive radioimmunoassay. The results showed a marginally...Full Text Available

1978-12-01

269

Characteristics of the molecular diversity of the outer membrane protein A gene of Haemophilus parasuis

The aim of the present study was to describe the physicochemical characteristics of streptococcal T antigen. T protein isolated from Streptococcus pyogenes type 12 (R53/1077, Colindale) and purified...Full Text Available

1978-09-01

270

CID755673 enhances mitogenic signaling by phorbol esters, bombesin and EGF through a protein kinase D-independent pathway

The molecular diversity of the gene encoding the outer membrane protein A (OmpA) of Haemophilus parasuis has been unclear. In this study, the structural characteristics, sequence types,...Full Text Available

2010-07-01

271

Biophysical characterization of recombinant proteins: A key to higher structural genomics success

Recently, CID755673 was reported to act as a highly selective inhibitor of protein kinase D (PKD). In the course of experiments using CID755673, we noticed that it exerted unexpected stimulatory...Full Text Available

2010-01-01

272

Binary Bacterial Toxins: Biochemistry, Biology, and Applications of Common Clostridium and Bacillus Proteins

Hundreds of genomes have been successfully sequenced to date, and the data are publicly available. At the same time, the advances in large-scale expression and purification of recombinant proteins have...Full Text Available

2010-10-01

273

Association of Protein Phosphatase 1?₁ with Spinophilin Suppresses Phosphatase Activity in a Parkinson Disease Model*

Certain pathogenic species of Bacillus and Clostridium have developed unique methods for intoxicating cells that employ the classic enzymatic “A-B” paradigm for protein toxins. The binary...Full Text Available

2004-09-01

274

Application of the PM6 semi-empirical method to modeling proteins enhances docking accuracy of AutoDock

Sustained nigrostriatal dopamine depletion increases the serine/threonine phosphorylation of multiple striatal proteins that play a role in corticostriatal synaptic plasticity, including Thr²⁸⁶...Full Text Available

2008-05-23

275

Analysis on the alterations of lens proteins by Vitex negundo in selenite cataract models

BackgroundMolecular docking methods are commonly used for predicting binding modes and energies of ligands to proteins. For accurate complex geometry and binding energy estimation,...Full Text Available

276

An automated procedure for detecting protein folds from sub-nanometer resolution electron density

PurposeCataract is the leading cause of blindness and is associated with oxidative damage and protein modification in the lens. In the present study, we have employed proteomic and...Full Text Available

277

Amyloid Oligomer Neurotoxicity, Calcium Dysregulation, and Lipid Rafts

The use of sub-nanometer resolution electron density as spatial constraints for denovo and ab-initio structure prediction requires knowledge of protein boundaries...Full Text Available

2010-06-01

278

Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

Amyloid proteins constitute a chemically heterogeneous group of proteins, which share some biophysical and biological characteristics, the principal of which are the high propensity to acquire an incorrect...Full Text Available

279

Albumin binding of insulins acylated with fatty acids: characterization of the ligand-protein interaction and correlation between binding affinity and timing of the insulin effect in vivo.

BackgroundThe production of high yields of recombinant proteins is an enduring bottleneck in the post-genomic sciences that has yet to be addressed in a truly rational manner. Typically...Full Text Available

280

Agonist-dependent up-regulation of thyrotrophin-releasing hormone receptor protein.

Albumin is a multifunctional transport protein that binds a wide variety of endogenous substances and drugs. Insulins with affinity for albumin were engineered by acylation of the epsilon-amino group...Full Text Available

1995-12-15

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281

Adhesion of DOPA-Functionalized Model Membranes to Hard and Soft Surfaces

To study the effect of agonist on the TRH (thyrotrophin-releasing hormone) receptor protein, an epitope-tagged receptor was stably expressed in HEK-293 cells (human embryonic kidney 293 cells) and receptor...Full Text Available

2004-06-15

282

Absence of Ataxin-3 Leads to Enhanced Stress Response in C. elegans

The adhesive proteins secreted by marine mussels form a natural glue that cures rapidly to form strong and durable bonds in aqueous environments. These mussel adhesive proteins contain an unusual...Full Text Available

2009-01-01

283

AMP-activated protein kinase (AMPK) cross-talks with canonical Wnt signaling via phosphorylation of ?-catenin at Ser 552

Ataxin-3, the protein involved in Machado-Joseph disease, is able to bind ubiquitylated substrates and act as a deubiquitylating enzyme in vitro, and it has been involved in the modulation of protein...Full Text Available

284

AMP-Activated Protein Kinase: A Stress-Responsive Kinase with Implications for Cardiovascular Disease

AMP-activated protein kinase (AMPK) is a key regulator of energy metabolism; its activity is regulated by a plethora of physiological conditions, exercises and many anti-diabetic drugs. Recent...Full Text Available

2010-04-23

285

AICD Overexpression in Neuro 2A Cells Regulates Expression of PTCH1 and TRPC5

AMP-activated protein kinase (AMPK) was initially viewed as energy sensor and activated by increased intracellular concentrations of AMP following nutrient deprivation. Physiological or pathological...Full Text Available

2010-04-01

286

A study in molecular contingency: glutamine phosphoribosylpyrophosphate amidotransferase is a promiscuous and evolvable phosphoribosylanthranilate isomerase

Amyloid precursor protein (APP), implicated in Alzheimer's disease, is a transmembrane protein of undetermined function. APP is cleaved by gamma-secretase that releases the APP intracellular domain...Full Text Available

287

A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning

SummaryThe prevalence of paralogous enzymes implies that novel catalytic functions can evolve on preexisting protein scaffolds. The weak secondary activities of proteins, which...Full Text Available

2008-03-21

288

A microscale protein NMR sample screening pipeline

The extreme strength and elasticity of spider silks originate from the modular nature of their repetitive proteins. To exploit such materials and mimic spider silks, comprehensive strategies...Full Text Available

2009-01-01

289

A global optimization algorithm for protein surface alignment

As part of efforts to develop improved methods for NMR protein sample preparation and structure determination, the Northeast Structural Genomics Consortium (NESG) has implemented an NMR screening pipeline for protein target selection, construct optimization, and buffer optimization, incorporating efficient microscale NMR screening of proteins using a micro-cryoprobe. The process is feasible because the newest generation probe requires only small amounts of protein, typically 30-200 ?g in 8-35 ?l volume. Extensive automation has been made possible by the combination of database tools, mechanization of key process steps, and the use of a micro-cryoprobe that gives excellent data while requiring little optimization and manual setup. In this perspective, we describe the overall process used by the NESG for screening NMR samples as part of a sample optimization process, assessing optimal construct design and ...

2010-01-01

290

A genome-wide study of PDZ-domain interactions in C. elegans reveals a high frequency of non-canonical binding

BackgroundA relevant problem in drug design is the comparison and recognition of protein binding sites. Binding sites recognition is generally based on geometry often combined with...Full Text Available

291

A gene encoding a protein modified by the phytohormone indoleacetic acid

BackgroundProteins may evolve through the recruitment and modification of discrete domains, and in many cases, protein action can be dissected at the domain level. PDZ domains are...Full Text Available

292

?Activation of the mitogen-activated protein kinase pathways by heat shock

We show that the expression of an indole-3-acetic acid (IAA)-modified protein from bean seed, IAP1, is correlated to the developmental period of rapid growth during seed development. Moreover, this...Full Text Available

2002-02-05

293

'Sheltered disruption' of Neurospora crassa MOM22, an essential component of the mitochondrial protein import complex.

In addition to inducing new transcriptional activities that lead within a few hours to the accumulation of heat shock proteins (Hsps), heat shock activates within minutes the major signaling transduction...Full Text Available

2002-04-01

294

[ ${\\alpha}$ ]-Amylase and Protein Tyrosine Phosphatase 1B Inhibitory of Some Vietnamese Medicinal Plants Used to Treat Diabetes

MOM22 is a component of the protein import complex of the mitochondrial outer membrane of Neurospora crassa. Using the newly developed procedure of 'sheltered disruption', we created a heterokaryotic...Full Text Available

1995-03-15

295

Uptake of Heavy Metal Ions by Water Dropwort (Oenanthe stolonifera DC.) and Identification of Its Heavy Metal-Binding Protein

Full Text Available

2007-12-01

296

The protein truncation caused by fusion of PEP-1 peptide and protective roles of transduced PEP-1-MsrA in skin cells

Full Text Available

1996-12-01

297

Prediction of Bacterial Proteins Carrying A Nuclear Localization Signal and Nuclear Targeting of HsdM from Klebsiella pneumoniae

Full Text Available

2011-04-01

298

Photolabelling reveals differences between cholera toxin and ricin toxin insertion mechanisms

Full Text Available

2009-10-01

299

Liquid nitrogen dewar for protein crystal growth

... carbon 14 cholera gels labelling membranes molecules proteins receptors

1982-07-28

300

Letter to the Editor: 1H, 13C and 15N chemical shift assignments of the capsid protein from Rous sarcoma virus

Gaseous Nitrogen Dewar apparatus developed by Dr. Alex McPherson of the University of California,

2001-01-01

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301

Interaction Of Human La Protein With The Internal Ribosome Entry Site Of Hepatitis C Virus : Functional Role In Mediating Internal Initiation Of Translation

No abstract prepared.

1999-11-15

302

Indian Institute of Science Theses & Dissertations (India)

Full Text Available

2004-07-01

303

Identification of Protective Brucella Antigens and their ...

Heavy Metals Effect on Animal Cells

... Considering these observations, it is most likely that Brucela proteins involved in protective immunity will preferentially stimulate INF-g producing T ...

1996-05-01

304

International Science & Technology Center (ISTC)

Studies of the Superheavy-metal Effect as a Stress-factor on Protein Biosynthesis and Cytoskeleton Integrity in Animal Cells

305

Functional characterization of a CCCH type zinc-finger protein gene OsZF2 by ectopic overexpression of the gene in rice

Free radicals and their transformations in irradiated proteins

Full Text Available

2009-03-01

306

Effect of protein malnutrition on the metabolism of bone collagen in albino rats

Experimental investigation data are systematized of free radical states and processes in irradiated proteins. The investigation is performed by the radiospectral methods. Results are discussed in detail of the study of free radicals electronic structure of amino acids, peptides and proteins formed by the action of ionizing radiation. The specificity is stressed of the study of monocrystalls of these compounds by the method of electronic paramagnetic resonance. The nature is also studied of primary centres formed under the effect of radiation on biologically important compounds and their subsequent reactions in solid and liquid solutions. Ion-radical states of different functional groups of the protein molecule are studied. Prospects of the study and the role of anion-radicals in biological processes are discusses.

307

Effect of Carbon Tetrachloride on the Changes of Xanthine Oxidase Activity in Rate Previously Fed Low or High Protein Diet

The effect of protein malnutrition on the metabolism of collagen in bone was studied in young female albino rats after a single injection of "3H-proline. Both specific and total radioactivities of hydroxyproline in the total collagen of the bone were found to decrease in the protein-deficient animals, indicating decreased rate of collagen synthesis. In the urine the amount of hydroxyproline excreted and total radioactivity of "3H-hydroxyproline were greatly decreased. The results of the present investigation therefore clearly indicate decreased synthesis and catabolism of collagen in bones of protein deficient animals compared to controls. (auth.).

1981-01-01

308

Turnover of whole body proteins and myofibrillar proteins in middle-aged active men

Full Text Available

1991-12-01

309

The Protein Kinase SnRK2.6 Mediates the Regulation of Sucrose Metabolism and Plant Growth in Arabidopsis^[W]^[OA]

Endurance-trained older men have a higher proportion of lean tissue and greater muscle cell oxidative capacity, reversing age-related trends and suggesting major changes in protein metabolism. In this study, protein turnover was determined in 6 middle-aged (52+/-1 yr) men who were well trained (VO_2 max 55.2+/-5.0 ml O_2/kg.min) and lean (body fat 18.9+/-2.8%, muscle mass 36.6+/-0.6%). The maintained habitual exercise while consuming 0.6, 0.9 or 1.2 g protein/kg.day for 10-day periods. N flux was measured from "1"5N in urea after oral "1"5N-glycine administration. Myofibrillar protein breakdown was estimated from urinary 3-methyl-histidine. Dietary protein had no effect on turnover rates, even when N balance was negative. Whole body protein synthesis was 3.60+/-0.12 g/kg.day and breakdown was 3.40+/-0.14 g/kg.day for all N intakes. Whole body ...

1986-04-13

310

Scyl1 Facilitates Nuclear tRNA Export in Mammalian Cells by Acting at the Nuclear Pore Complex

In higher plants, three subfamilies of sucrose nonfermenting-1 (Snf1)-related protein kinases have evolved. While the Snf1-related protein kinase 1 (SnRK1) subfamily has been shown to share pivotal...Full Text Available

2010-05-01

311

Relationships of Quality Characteristics with Size Exclusion HPLC Chromatogram of Protein Extract in Soft-White Winter Wheats.

Scyl1 is an evolutionarily conserved N-terminal protein kinase-like domain protein that plays a role in COP1-mediated retrograde protein trafficking in mammalian cells. Furthermore, loss of Scyl1 function...Full Text Available

2010-07-15

312

Protective Effects of a Human 18-Kilodalton Cationic Antimicrobial Protein (CAP18)-Derived Peptide against Murine Endotoxemia

This study investigated relationships between molecular weight distributions of unreduced grain proteins and grain, flour, and end-use quality characteristics of soft white winter wheats grown in Oregon. Absorbance area and area % values of protein fractions separated by size exclusion HPLC (SE-HPL...

313

Influence of the high-affinity growth hormone (GH)-binding protein on plasma profiles of free and bound GH and on the apparent half-life of GH. Modeling analysis and clinical applications.

CAP18 (an 18-kDa cationic antimicrobial protein) is a granulocyte-derived protein that can bind lipopolysaccharide (LPS) and inhibit various activities of LPS in vitro. The present study examined the...Full Text Available

1998-05-01

314

Identification of a Drosophila G protein alpha subunit (dGq alpha-3) expressed in chemosensory cells and central neurons.

The discovery of a specific high-affinity growth hormone (GH) binding protein (GH-BP) in plasma adds complexity to the dynamics of GH secretion and clearance. Intuitive predictions are that such a protein...Full Text Available

1993-02-01

315

Gamma interferon induces rapid and coordinate activation of mitogen-activated protein kinase (extracellular signal-regulated kinase) and calcium-independent protein kinase C in human monocytes.

We have identified another Drosophila GTP-binding protein (G protein) alpha subunit, dGq alpha-3. Transcripts encoding dGq alpha-3 are derived from alternative splicing of the dGq alpha locus previously...Full Text Available

1995-12-05

316

Dual Functions of ASCIZ in the DNA Base Damage Response and Pulmonary Organogenesis

Gamma interferon plays an important role in regulating the functional properties of mononuclear phagocytes. In the present study, the role of activated protein kinases in the mechanism of action of...Full Text Available

1994-07-01

317

Chemical and Immunological characterization of the major structural protein (p28) of MMC-1, a rhesus monkey endogenous type C virus: homology with the major structural protein of avian reticuloendotheliosis virus.

Zn²⁺-finger proteins comprise one of the largest protein superfamilies with diverse biological functions. The ATM substrate Chk2-interacting Zn²⁺-finger protein...Full Text Available

2010-10-01

318

Study of protein-protein interactions in under saturated and supersaturated lysozyme solutions in heavy water as a function of temperature; Etude des interactions proteine-proteine en solutions sous-saturees et sursaturees de lysozyme dans l`eau lourde en fonction de la temperature

The major core protein (p28) of MMC-1, an endogenous type C virus of the rhesus monkey (Macaca mulatta), was purified and subjected to structural and immunological analyses. The NH2-terminal amino acid...Full Text Available

1981-09-01

319

Observation of the study of protein metabolism in relation to nutrition

We have studied freshly prepared lysozyme solutions in heavy water for two NaCl concentrations as a function of temperature. Lysozyme solubilities in this solvent are determined by static light scattering. By small angle neutron scattering, we evidence that interactions between lysozyme molecules are characterized by a second virial coefficient A{sub 2} whether the solution is under-saturated or supersaturated. From the variation of A{sub 2} as a function of temperature we have evaluated the enthalpy corresponding to the interaction between lysozyme molecules. We show that the interactions between protein molecules are higher in heavy water than in light water. (authors). 13 refs., 3 figs.

1996-04-01

320

Identification of a functional nuclear export signal in the green fluorescent protein asFP499

It is a useful hypothesis to thing of the reaction to protein deprivation as made up of two parts. First there is an initial adaptation involving rapid losses of protein. This is followed by slow continuing depletion. When considering two organ groups, such as the liver and muscle, it appears that the curve of urinary nitrogen excretion reflects the different parts they play in these two phases. The changes in the relative amounts of protein in different organs results in an alteration in the pattern of body protein at the level of the organ, the tissue and the cell. These changes are themselves the result of altered rates of synthesis and breakdown. The questions that must be asked and hopefully will be answered with the help of isotopes, are how these changes are brought about, how they are inter-related and controlled, and what is their significance? This paper contains a discussion of the few ...

1965-06-01

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321

Artificial neural network modeling of physicochemical changes of shrimp during boiling

The green fluorescent protein (GFP) asFP499 from Anemonia sulcata is a distant homologue of the GFP from Aequorea victoria. We cloned the asFP499 gene into a mammalian expression vector and showed that this protein was expressed in the human lymphoblast cell line Ramos RA1 and in the embryonic kidney 293T cell line (HEK 293T). In HEK 293T cells, asFP499 was localized mainly in the cytoplasm, suggesting that the protein was excluded from the nucleus. We identified _1_9_4LRMEKLNI_2_0_1 as a candidate nuclear export signal in asFP499 and mutated the isoleucine at position 201 to an alanine. Unlike the wildtype form, the mutant protein was distributed throughout the cytoplasm and nucleus. This is First report of a GFP that contains a functional NES.

2006-04-21

322

British Library Electronic Table of Contents (United Kingdom)

Frozen boiled shrimp and dried shrimp are among the high-value fishery products of Thailand. During the production of these products boiling is one of the most important steps that affects significantly the product physicochemical properties, especially the quantity and quality of proteins, which in turn affect other apparent properties perceived by consumers. The protein changes are, however, difficult to evaluate comparing to other typical physical properties of shrimp. The objective of this study was therefore to develop an artificial neural network (ANN) model to predict the protein changes of shrimp in terms of protein loss and protein denaturation as a function of the boiling conditions, namely, concentration of salt solution and boiling time, as well as a rather easily determined ch...

2012-01-01

323

Amyloid oligomers: spectroscopic characterization of amyloidogenic protein states

British Library Electronic Table of Contents (United Kingdom)

It is assumed that protein fibrils manifested in amyloidosis result from an aggregation reaction involving small misfolded protein sequences being in an `oligomeric' or `prefibrillar' state. This review covers recent optical spectroscopic studies of amyloid protein misfolding, oligomerization and amyloid fibril growth. Although amyloid fibrils have been studied using established protein-characterization techniques throughout the years, their oligomeric precursor states require sensitive detection in real-time. Here, fluorescent staining is commonly performed using thioflavin T and other small fluorescent molecules such as 4-(dicyanovinyl)- julolidine and 1-amino-8-naphtalene sulphonate that have high affinity to hydrophobic patches. Thus, populated oligomeric intermediates and related `pre...

2010-01-01

324

Altered protein phosphorylation in sciatic nerve from rats with streptozocin-induced diabetes

Suppressive effects of cadmium on neurons and affected proteins in cultured developing cortical cells

The effect of experimental diabetes on the phosphorylation of proteins in the rat sciatic nerve was studied. Nerves from animals made diabetic with streptozocin were incubated in vitro with (/sup 32/P)orthophosphate and divided into segments from the proximal to the distal end, and proteins from each segment were then separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The principal labeled species were the major myelin proteins, P0, and the basic proteins. After 6 wk of diabetes, the incorporation of isotope into these proteins rose as a function of distance along the nerve in a proximal to distal direction and was significantly higher at the distal end compared with incorporation into nerves from age-matched controls. The overall level of isotope uptake was similar in nerves from diabetic animals and weight-matched controls. The distribution of /sup 32/P among ...

1987-11-01

325

Euglena light-harvesting chlorophyll A/B binding protein (LHCP) synthesized as an unusually large precursor

The purpose of the present study was to examine the in vitro effects of low-dose cadmium (Cd) on developing cortical cells. The cortical cells removed from fetuses (embryonic day 15) were treated with 10 nM of Cd for 24 h. The effects of Cd on dendritic and synaptic development were immunocytochemically observed with anti-microtubule associated protein-2 (MAP2) and anti-synapsin I antibodies, respectively. Administration of Cd suppressed dendritic as well as synaptic development at 10 nM. By two-dimensional gel electrophoresis and liquid chromatography-tandem mass spectrometric (LC/MS/MS) analysis, we identified three proteins with different expression after Cd-treatment; dihydropyrimidinase-related protein 2 (DRP-2/CRMP-2), 14-3-3-epsillon and calmodulin (CaM). Though the number of identified proteins was small, these proteins are known to be involved in neuronal development. The ...

2008-11-20

326

The Effects of Surface Chemistry on the Properties of Proteins Confined in Nano-porous Materials

Light increased the rate of LHCP synthesis as measured by pulse-labeling with /sup 35/SO/sub 4/ and immunoprecipitation with antibody specific for Euglena LHCP. In addition to the mature LHCP, 26,000 daltons, the LHCP specific antibody immunoprecipitated large amounts of several proteins having molecular weights of approximately 100,000. On immunoblots of immunoprecipitated unlabeled protein, the antibody only detected the mature LHCP suggesting that the high molecular weight proteins are not LHCP aggregates produced during immunoprecipitation. After a 10 min pulse with /sup 35/SO/sub 4/, the 100,000 dalton proteins constituted over 80% of the immunoprecipitated material. In a subsequent chase, the radioactivity in the 100,000 dalton proteins decreased and the radioactivity in the mature LHCP increased suggesting a precursor-product relationship. After a 35 minute chase, the mature ...

1987-04-01

327

Single protein omission reconstitution studies of tetracycline binding to the 30S subunit of Escherichia coli ribosomes

The entrapment of proteins using the sol-gel route provides a means to retain its native properties and artificially reproduce the molecular crowding and confinement experienced by proteins in the cell allowing investigation of the physico-chemical and structural properties of biomolecules at the biotic/abiotic interface. The biomolecules are spatially separated and 'caged' in the gel structure but solutes can freely permeate the matrix. Thus, properties such as the folding of ensembles of individual molecules can be examined in the absence of aggregation effects that can occur in solution studies. Green fluorescent protein from Aequorea coerulescens was used as a model protein to examine the unfolding/re-folding properties of protein in silica gels. The recombinant protein was isolated and purified from Escherichia coli extracts by cell lysis, ...

2007-09-01

328

Species abundance distributions in neutral models with immigration or mutation and general lifetimes

In previous work the authors showed that on photolysis of Escherichia coli ribosomes in the presence of ({sup 3}H)tetracycline (TC) the major protein labeled is S7, and they presented strong evidence that such labeling takes place from a high-affinity site related to the inhibitory action of TC. In this work they use single protein omission reconstitution (SPORE) experiments to identify those proteins that are important for high-affinity TC binding to the 30S subunit, as measured by both cosedimentation and filter binding assays. With respect to both sedimentation coefficients and relative Phe-tRNA{sup Phe} binding, the properties of the SPORE particles they obtain parallel very closely those measured earlier, with the exception of the SPORE particle lacking S13. A total of five proteins, S3, S7, S8, S14, and S19, are shown to be important for TC binding, with the largest effects seen on omission of ...

1990-06-05

329

Pseudocraters as indicators of ground ice on Mars

We consider a general, neutral, dynamical model of biodiversity. Individuals have i.i.d. lifetime durations, which are not necessarily exponentially distributed, and each individual gives birth independently at constant rate \\lambda. We assume that types are clonally inherited. We consider two classes of speciation models in this setting. In the immigration model, new individuals of an entirely new species singly enter the population at constant rate \\mu (e.g., from the mainland into the island). In the mutation model, each individual independently experiences point mutations in its germ line, at constant rate \\theta. We are interested in the species abundance distribution, i.e., in the numbers, denoted I_n(k) in the immigration model and A_n(k) in the mutation model, of species represented by k individuals, k=1,2,...,n, when there are n individuals in the total population. In the immigration model, we prove that the numbers (I_t(k);k\\ge 1) of species ...

2010-01-01

330

Plutonic and metamorphic xenoliths from the Cascada Tuff, Chihuahua, Mexico, as evidence indicating the composition of the basement rocks beneath the Sierra Madre Occidental

Positive identification of Martian pseudocraters would be a strong indication of past occurrence of ice at or near the surface of Mars. The basis for suggesting that small cones on Mars are pseudocraters includes: (1) small size, (2) abundant but patchy distribution on what appear to be volcanic plains, (3) presence of other features suggestive of surface or subsurface ice, (4) morphological similarities to Icelandic pseudocraters, and (5) the similarity in distribution of crater/cone diameter ratios to Icelandic pseudocraters. This last morphometric parameter may be the most important, since other possible small terrestrial volcanic analogs have very different crater/cone diameter ratio distributions. In a survey of the available high resolution Viking Orbiter imagery, abundant fields of possible pseudocraters were found. However, only a small fraction of the plains forming units imaged at high resolution were found to contain the small cones. ...

1987-05-01

331

Ongoing collapse of coral-reef shark populations.

The Sierra Madre Occidental of western Mexico is composed dominantly of Mid-Tertiary felsic and subordinate mafic volcanic rocks with only sparse outcrops of non-volcanic rocks. There are widely scattered but small exposure of plutonic rocks but regionally metamorphosed rocks are not known to occur in the Sierra. To this date the only known area where plutonic and metamorphic xenoliths have been found is near the village of Basaseachic in western Chihuahua where thick outcrops of the Cascada Tuff occur. The xenoliths are the only known occurrence of regionally metamorphosed rocks for a distance of about 400 km between exposures of Precambrian rocks to the west in Sonora and the east in central Chihuahua. Non-volcanic xenoliths from a few cm to about one meter in diameter occur most abundantly in the upper portions of the Cascada Tuff. They can be divided into four main groups in decreasing order of abundance as follows: (1) coarse-grained ...

1985-01-01

332

On the Insignificance of Photochemical Hydrocarbon Aerosols in the Atmospheres of Close-in Extrasolar Giant Planets

Marine ecosystems are suffering severe depletion of apex predators worldwide; shark declines are principally due to conservative life-histories and fisheries overexploitation. On coral reefs, sharks are strongly interacting apex predators and play a key role in maintaining healthy reef ecosystems. Despite increasing fishing pressure, reef shark catches are rarely subject to specific limits, with management approaches typically depending upon no-take marine reserves to maintain populations. Here, we reveal that this approach is failing by documenting an ongoing collapse in two of the most abundant reef shark species on the Great Barrier Reef (Australia). We find an order of magnitude fewer sharks on fished reefs compared to no-entry management zones that encompass only 1% of reefs. No-take zones, which are more difficult to enforce than no-entry zones, offer almost no protection for shark populations. Population viability models of whitetip and gray reef sharks ...

2006-12-01

333

Magnetic fusion reactor economics

The close-in extrasolar giant planets (CEGPs) reside in irradiated environments much more intense than that of the giant planets in our solar system. The high UV irradiance strongly influences their photochemistry and the general current view believed that this high UV flux will greatly enhance photochemical production of hydrocarbon aerosols. In this letter, we investigate hydrocarbon aerosol formation in the atmospheres of CEGPs. We find that the abundances of hydrocarbons in the atmospheres of CEGPs are significantly less than that of Jupiter except for models in which the CH$_4$ abundance is unreasonably high (as high as CO) for the hot (effective temperatures $\\gtrsim 1000$ K) atmospheres. Moreover, the hydrocarbons will be condensed out to form aerosols only when the temperature-pressure profiles of the species intersect with the saturation profiles--a case almost certainly not realized in the hot CEGPs atmospheres. Hence our models show ...

2004-01-01

334

Intersection model for estimating sea otter mortality from the Exxon Valdez oil spill along the Kenai Peninsula, Alaska. Marine mammal study 6-5. Exxon Valdez oil spill state/federal natural resource damage assessment final report

An almost primordial trend in the conversion and use of energy is an increased complexity and cost of conversion systems designed to utilize cheaper and more-abundant fuels; this trend is exemplified by the progression fossil fission {yields} fusion. The present projections of the latter indicate that capital costs of the fusion ``burner`` far exceed any commensurate savings associated with the cheapest and most-abundant of fuels. These projections suggest competitive fusion power only if internal costs associate with the use of fossil or fission fuels emerge to make them either uneconomic, unacceptable, or both with respect to expensive fusion systems. This ``implementation-by-default`` plan for fusion is re-examined by identifying in general terms fusion power-plant embodiments that might compete favorably under conditions where internal costs (both economic and environmental) of fossil and/or fission are not as great as is needed to justify ...

1995-12-01

335

Independent Emission and Absorption Abundances for Planetary Nebulae

The authors developed an analytical model (intersection model) to estimate the exposure of sea otters (Enhydra lutris), to oil from the Exxon Valdez oil spill. The authors applied estimated and assumed exposure dependent mortality rates to the Kenai Peninsula sea otter population to provide examples of the application of the model in estimating sea otter mortality. The intersection model requires three distinct types of data: (1) distribution, abundance, and movements of oil, (2) abundance and distribution of sea otters, and (3) sea otter mortality rates relative to oil exposure. Initial output of the model is an estimate of exposure of otters to oil. Exposure is measured in amount and duration of oil near an otter`s observed location (intersections). The authors provide two examples of the model using different assumptions about the relation between exposure and mortality. Because of an apparent non-linear relation between the degree of oiling ...

1995-06-01

336

In situ studies with Asian clams (Carbacula fluminea) detect acid mine drainage and nutrient inputs in low-order streams

Emission-line abundances have been uncertain for more than a decade due to unexplained discrepancies in the relative intensities of the forbidden lines and weak permitted recombination lines in planetary nebulae (PNe) and H II regions. The observed intensities of forbidden and recombination lines originating from the same parent ion differ from their theoretical values by factors of more than an order of magnitude in some of these nebulae. In this study we observe UV resonance line absorption in the central stars of PNe produced by the nebular gas, and from the same ions that emit optical forbidden lines. We then compare the derived absorption column densities with the emission measures determined from ground-based observations of the nebular forbidden lines. We find for our sample of PNe that the collisionally excited forbidden lines yield column densities that are in basic agreement with the column densities derived for the same ions from the UV absorption lines. ...

2008-01-01

337

Establishing a Connection Between Active Region Outflows and the Solar Wind: Abundance Measurements with EIS/Hinode

This study evaluates the correlation between transplanted Asiatic clam and indigenous community responses to acid mine drainage and nutrient loading in first-to-third-order streams, by comparing the toxicological endpoints of clam survival and growth with benthic macro-invertebrate community indices as community responses to both acid mine drainage and nutrient loading. Clam survival was found to be positively correlated with water column pH and negatively correlated with conductivity and metal concentrations. There was also a positive correlation with the relative abundance of the macro-invertebrate Ephemeroptera, the most sensitive taxonomic group, to acid mine drainage in this watershed. No correlation was found between clam growth and acid mine drainage inputs, but there was evidence of positive correlation with nitrate concentrations and the relative abundance of collector-filterer functional feeding groups. These results suggest that clam ...

2001-03-01

338

Carbonate shoreline sedimentation in San Andres Formation, Lincoln County, New Mexico

One of the most interesting discoveries of the X-ray Telescope and EUV Imaging Spectrometer (EIS) on board the Hinode solar observatory is the presence of persistent high temperature high speed outflows from the edges of active regions. Measurements by EIS indicate that the outflows reach velocities of 50 km/s with spectral line asymmetries approaching 200 km/s. It has been suggested that these outflows may lie on open field lines that connect to the heliosphere, and that they could potentially be a significant source of the slow speed solar wind. A direct link has been difficult to establish, however. In this letter, we use EIS measurements of spectral line intensities that are sensitive to changes in the relative abundance of Si and S as a result of the first ionization potential (FIP) effect, to measure the chemical composition in the outflow regions of AR 10978 over a period of 5 days in December 2007. We find that Si is always enhanced over S by a factor of ...

2010-01-01

339

[Changes in ecological features of soils after controlled fires in forests defoliated by the Siberian moth in the southern taiga subzone of the Enise? Region, Siberia].

The Permian San Andres Formation of late Leonardian-early Guadalupian age is a significant hydrocarbon producer and crops out over a large area in south-central New Mexico. However, since its definition in 1909, the age and depositional environment of this unit have been questioned. In 1971 in Pecos County, Vincent Kelly divided the unit into three members: Rio Bonito, Bonney Canyon, and Fourmile Draw. The lowest member, although named for the Rio Bonito, has a type section designated at Sunset along U.S. Highway 70/380. Two miles east of Sunset, near Riverside, on the Rio Bonito, the Rio Bonito Member is exposed in a long series of roadcuts. Petrographic investigation of these rocks reveals an alternating sequence of high to low-energy intertidal to subtidal facies marked by oolitic and fossiliferous dolocalcarenite interbedded with dolomitic mudstone. The unit has been recrystallized and stylolites are abundant, as is dolomite and nodular mosaic anhydrite. These ...

1986-03-01

340

Ultrasensitive laser isotope analysis in an ion-storage ring

Data on the postfire dynamics of soil properties in the foci of Siberian moth population outbreaks are considered. It has been shown that controlled fires set in pest-defoliated forests result in the loss of approximately 75% of carbon and 50% of nitrogen from the forest litter through their emission into the atmosphere and in the enrichment of the upper soil horizons with potassium and phosphorus (this concerns both total and movable forms). Microbiological processes in the organogenic horizon undergo significant transformation, the density of microarthropods decreases, and the abundance of mites becomes hundreds of times lower. PMID:15354965

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

341

Synthetic fuels from coal

We propose a novel method for ultrasensitive isotope analysis that combines magnetic mass selection, resonant charge-exchange neutralization, and resonant laser ionizaion. Our method attains high isotopic abundance selectivity by means of continuous multistage separation of ions stored in a small ring. For the environmentally interesting case of /sup 90/Sr versus /sup 88/Sr we estimate that sensitivity better than 10/sup -15/ for a throughput of 10/sup 13/ atoms/sec and an efficiency (after the ion source) greater than 10% are readily achievable.

1985-09-01

342

ROSAT observations of the RSCVn binary sigma Geminorum

Alternative sources of fuel, especially for transport, continue to be of interest, as the availability of petroleum is likely to decline in the foreseeable future. Coal is a perfect alternative since it is abundant and can readily be refined to products similar to petroleum fuels. This work details the continuing research program undertaken by the European Commission, bringing together results of studies by international experts in the field. The editors have synthesized vast amounts of recent literature to produce a state-of-the-art review covering synthetic processes, single and two-stage hydrogenation processes, processing of coal with heavy oil and catalysis.

1988-01-01

343

DEFF Research Database (Denmark)

X-ray observations of the RSCVn system sigma Geminorum have been undertaken with the ROSAT observatory. Several spectra of very good signal-to-noise ratio were obtained. Spectral fitting using metal abundances amounting to 50% of solar values reveal two temperature components at 2 MK and 12 MK. Previous EXOSAT observations showed another component at 40 MK. Particular interest is focused on the temporal variations of the X-ray emission. It is found that variations occur on time scales ranging from years to hours and minutes.

1997-01-01

344

PBAR: A superconducting magnet spectrometer for cosmic ray antiproton studies

Nutrient regulation of the saprotroph to parasite transition in Pochonia chlamydopsoria, a soil microbial inoculant for nematode control

We describe the PBAR balloon-borne magnet spectrometer flown on August 13-14, 1987 to measure the abundance of cosmic ray antiprotons in the energy interval 100-1580 MeV at the top of the atmosphere. The limits first reported have been improved to an overall limit of anti p/p<2.0x10{sup -5} (85% CL). We summarize the overall design and performance of the PBAR spectrometer, which had the unique ability to establish the mass of each singly charged cosmic ray, as well as to reject spurious antimatter candidates caused by hard scatterings within the instrument. (orig.).

1990-09-15

345

Environmental Research Database

DescriptionThe nematophagous fungus, Pochonia chlamydosporia is a facultative parasite that has been developed through collaborative research between Rothamsted and the Centro Nacional de Sanidad Agropecuaria, Havana, Cuba, as the biocontrol agent Klamic against root-knot nematodes in tropical soils. The abundance of this fungus in soil is not necessarily related to its effectiveness as a biological control agent and its parasitic activity against nematode eggs is related to its nutrition and physiological [continued...

2011-01-31

346

Light elements in massive single and binary stars

It is petrochemical of the waste plastic; Hai purasutikku no yuka

We highlight the role of the light elements (Li, Be, B) in the evolution of massive single and binary stars, which is largely restricted to a diagnostic value, and foremost so for the element boron. However, we show that the boron surface abundance in massive early type stars contains key information about their foregoing evolution which is not obtainable otherwise. In particular, it allows to constrain internal mixing processes and potential previous mass transfer event for binary stars (even if the companion has disappeared). It may also help solving the mystery of the slowly rotating nitrogen-rich massive main sequence stars.

2010-01-01

347

Hydrocarbon and mineral resources of the Uinta Basin, Utah and Colorado

It becomes a cause of the air pollution by the generation of hydrogen chloride, when it is incinerated, when it decays, even if the plastic which becomes spent is buried. Every year, it is abundantly discharged and accumulates. Countries and enterprises, etc. carry out the examination for non-polluting processing and resource recycling of the waste plastic for material recycling and development by methods such as thermal recycling, practical application. In this paper, the following are examined: Plastic output and transition of the situation of the disposal quantity, resource recycling and problem. It wants to explain the challenge of more and more efficient resource recycling. (NEDO)

1999-11-01

348

Gasoline from coal: the pioneering South African experience

The Uinta Basin is an important hydrocarbon producing area, with extensive resources of hydrocarbon, bitumen, and fossil fuels. Gilsonite, tar sands, oil shale and coal are found in abundance with the cretaceous and Tertiary sedimentary rocks. The 25 papers include discussion on: leasing; thermal maturity of rocks and hydrocarbon deposits; influence of source rock type, thermal maturity and migration on composition and distribution of the natural gas; drilling; geology; structural evolution of gilsonite dykes; reclamation of coal mines; oil shale resources; and uranium mining.

1992-01-01

349

Gasoline from coal: the pioneering South African experience

The fact that South Africa had no commercially significant deposits of crude oil necessitated the early development of production of gasoline from coal which was present in abundance in low to medium grade in thick seams, the development of the process since the early 1950s is discussed. Both the Fischer Tropsch and Lurgi processes are used, and three large plants named Sasol One, Two, and Three have been completed. A flow diagram for the Lurgi process as used in these plants is included. Typical products and their properties resulting from the Sasol-type Synthol operation are presented in tabular form. Marketing considerations and economics of production of the Sasol products are discussed briefly. (BLM)

1982-03-01

350

Evolution of the primary components of massive binary stars in the case of Roche lobe overflow after main-sequence evolution

The fact that South Africa had no commercially significant deposits of crude oil necessitated the early development of production of gasoline from coal which was present in abundance in low to medium grade in thick seams, the development of the process since the early 1950s is discussed. Both the Fischer Tropsch and Lurgi processes are used, and three large plants named Sasol One, Two, and Three have been completed. A flow diagram for the Lurgi process as used in these plants is included. Typical products and their properties resulting from the Sasol-type Synthol operation are presented in tabular form. Marketing considerations and economics of production of the Sasol products are discussed briefly. (BLM)

1982-03-01

351

Effects of the variation of fundamental constants on Pop III stellar evolution

The components with masses 32 and 64M _s_u_n, evolved with the matter mixing in the semiconvective zone and filling their Roche lobes, after the main-sequence evolution overflow their Roche lobes and lose matter during the first part of the helium core burning. After the overflow end, the components lose the matter by stellar wind. The components shrink in the nuclear timescale. At first, they have the blue supergiant character with anomalous CNO abundance, then - the Wolf-Rayet stars character.

352

Biomass production in Florida

The effect of variations of the fundamental constants on the thermonuclear rate of the triple alpha reaction, "4He(#alpha##alpha#, #gamma#)"1"2C, that bridges the gap between "4He and "1"2C is investigated. We have followed the evolution of 15 and 60 M#centre dot# zero metallicity stellar models, up to the end of core helium burning. They are assumed to be representative of the first (Population III) stars. The calculated oxygen carbon abundances resulting from helium burning can then be used to constrain the variation of the fundamental constants.

2010-08-12

353

Atmospheric carboxylic acids in southern California

Florida posseses climatic, land, and water resources favorable for abundant biomass production. Therefore, a statewide program has been initiated to determine adapted species for the available array of production sites. Plant resources under investigation include woody, aquatic, grasses, hydrocarbon, and root crop species. The goal is to produce a continuous stream of biomass for the various biofuel conversion options. Preliminary yields from energy cropping experiments range from about 10 to nearly 90 metric tons per hectare per year, depending on the crop and the production systems employed. (Refs. 15).

1981-08-01

354

Achievements in testing of the MGA and FRAM isotopic software codes under the DOE/NNSA-IRSN cooperation of gamma-ray isotopic measurement systems

Intensive measurements of organic acids, inorganic acids, aldehydes and peroxyacetyl nitrate (PAN) in urban air were carried out at a Southern California smog receptor site, Glendora, CA, August 12-22, 1986. The objective of this project was to augment the data base regarding organic acids in urban air and to assess their role with emphasis on ambient levels, diurnal variations, phase distribution, emissions, in-situ formation and removal. The relative abundance of organic acids and inorganic acids was determined for the first time from simultaneous measurements carried out during this study.

1988-09-01

355

The ''nuclear car wash'': a scanner to detect illicit special nuclear material in cargo containers

DOE/NNSA and IRSN collaborated on a study of gamma-ray instruments and analysis methods used to perform isotopic measurements of special nuclear materials. The two agencies agreed to collaborate on the project in response to inconsistencies that were found in the various versions of software and hardware used to determine the isotopic abundances of uranium and plutonium. IRSN used software developed internally to test the MGA and FRAM isotopic analysis codes for criteria used to stop data acquisition. The stop-criterion test revealed several unusual behaviors in both the MGA and FRAM software codes.

2009-01-01

356

p53 protein in aggressive and non-aggressive basal cell carcinoma.

There is an urgent need to improve the reliability of screening cargo containers for illicit nuclear material that may be hidden there for terrorist purposes. A screening system is described for detection of fissionable material hidden in maritime cargo containers. The system makes use of a low intensity neutron beam for producing fission; and the detection of the abundant high-energy #gamma# rays emitted in the #beta#-decay of short-lived fission products and #beta#-delayed neutrons. The abundance of the delayed #gamma# rays is almost an order of magnitude larger than that of the delayed neutrons normally used to detect fission and they are emitted on about the same time scale as the delayed neutrons, i.e., #approx#1 min. The energy and temporal distributions of the delayed #gamma# rays provide a unique signature of fission. Because of their high energy, these delayed #gamma# rays penetrate loW--Z cargoes much more readily than the delayed ...

2004-01-28

357

Proteomic Analysis of Proteins Secreted by HepG2 Cells Treated with Butyl Benzyl Phthalate

Basal cell carcinoma (BCC) is the most frequent cutaneous neoplasm, with a generally favorable clinical behavior. Sometimes, indeed, it recurs after therapy and/or metastasizes. As point mutations in the coding sequence of the p53 tumor suppressor gene have been implicated in the progression of many human tumors, we studied the expression of p53 protein on this neoplasia. We tested immunohistochemically the positivity for p53 protein (NCL-p53-CM1, YLEM) on 19 cases of morphologically "non aggressive" BCC (BCC1) and on 19 "aggressive" BCC (BCC2), all with one or more relapses and 3 with distant metastases also. Results were related to clinico-pathological and follow-up data. All but one BCC2 were found positive for p53 protein. Conversely, only 2 cases of BCC1 exhibited low immunoreactivity for p53 protein, with high statistical differences between the two groups. No correlation was found between the ...

1993-10-01

358

British Library Electronic Table of Contents (United Kingdom)

Proteomic changes in proteins secreted by human hepatocellular carcinomas (HepG2) cells exposed to butyl benzyl phthalate (BBP) were evaluated. HepG2 cells were treated with three different concentrations of BBP (0, 10, or 25 ?M) for 24 or 48 h. Following incubation, the cells were subjected to proteomic analysis using two different pI ranges (4-7 and 6-9) and large-size two-dimensional gel electrophoresis. Results showed resolution of a total of 2776 protein spots. Of these, 29, including 19 upregulated and 10 downregulated proteins, were identified by electrospray ionization-mass spectrometry-mass spectrometry (ESI-MS/MS). Among these, the identities of cystatin C, Rho guanine nucleotide dissociation inhibitor, gelsolin, DEK protein, Raf kinase inhibitory protein, triose phosphate ...

2010-01-01

359

Macronutrient self-selection in Solea senegalensis fed macronutrient diets and challenged with dietary protein dilutions

British Library Electronic Table of Contents (United Kingdom)

Macronutrient self-selection and responses to dietary protein dilution were investigated in Senegalese sole, a flatfish species of interest for Mediterranean aquaculture. Eight groups of six sole were distributed among 75-l tanks and allowed to feed on three experimental diets composed of pure macronutrients: PC (75% crude protein and 25% carbohydrate), PF (75% crude protein and 25% crude fat) and PFC (10% crude protein, 45% crude fat and 45% carbohydrate). Choosing from the above diets, the sole selected a diet containing 68.0% crude protein, 15.7% crude fat and 16.3% carbohydrate. When the PF diet was diluted 50% with cellulose, the fish increased their feed intake to sustain the energy intake (5.0?kJ/kg BW/day). Their selection of fat remained unchanged, while carbohydrate selection sig...

2009-01-01

360

Ligand carrier protein genes expressed in larval chemosensory organs of Bombyx mori

British Library Electronic Table of Contents (United Kingdom)

Expressed sequence tags (ESTs) of the maxillary galea of the silkworm were analyzed to identify proteins involved in food selection systems. From the 1251 redundant genes of the ESTs, we identified 7 odorant-binding protein-like genes (bmObpL), 6 takeout-like genes (bmToL), and 6 chemosensory protein genes (bmCsp). Quantitative RT-PCR analysis indicated that bmObpL1, bmObpL2, bmObpL3, bmObpL5, bmToL1, bmToL3, and bmorCsp15 were predominantly expressed in the larval oral appendages, such as the maxilla, labrum, labium and antenna. Immunocytochemical analysis indicated that the proteins of bmObpL1, bmObpL3, and bmToL1 were localized in the gustatory chemosensilla on the maxillary galea and olfactory sensilla in the antenna. The proteins encoded by bmObpL1 and bmObpL3 were detected in the gus...

2011-01-01

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361

Human complement protein C99 is a calcium binding protein

Human complement protein C9 is shown to be a metalloprotein that binds 1 mol of Ca"2"+/mol of C9 with a dissociation constant of 3 #mu#m as measured by equilibrium dialysis. Incubation with EDTA removes the bound calcium, resulting in a apoprotein with decreased thermal stability. This loss in stability leads to aggregation and, therefore, to loss of hemolytic activity upon heating to a few degrees above the physiological temperature. Heat-induced aggregation of apoC9 can be prevented by salts that stabilize proteins according to the Hofmeister series of lyotropic ions, suggesting that the ion in native C9 may ligand with more than one structural element of domain of the protein. Ligand blotting indicates that the calcium binding site is located in the amino-terminal half of the protein. Removal of calcium by inclusion of EDTA in assay mixtures has no effect on the hemolytic activity of C9, and its ...

362

Amyloidosis

A single amino acid substitution results in a retinoblastoma protein defective in phosphorylation and oncoprotein binding

The subjects covered in this Symposium range through almost every clinical medical specialty. From an average of one paper in each of the past three Symposiums, the explosive interest in cerebral amyloidosis has led to the presentation of 12 papers on this subject in the present volume. The genetically predisposed familial amyloidotic processes, such as the polyneuropathies and familial Mediterranean fever have also stimulated extensive and intriguing investigations which have revealed the striking effect of a single amino acid substitution in transforming a normal protein into a lethal ''amyloidogenic'' one. This Symposium clearly depicts the advances since the first amyloid fibril protein was definitively identified and defined 14 years ago. Since all amyloid fibril proteins so far described are variants of normal proteins, attention to gene abnormalities now becomes a significant focus as well as the ...

1984-11-09

363

Vibrational Circular Dichroism: A developing spectroscopic technique for peptide and protein conformational studies

The authors have previously identified a small-cell lung cancer cell line (NCI-H209) that expresses an aberrant, underphosphorylated form of the retinoblastoma protein RB1. Molecular analysis of RB1 mRNA from this cell line revealed a single point mutation within exon 21 that resulted in a nonconservative amino acid substitution (cysteine to phenylalanine) at codon 706. Stable expression of this mutant RB1 cDNA in a human cell line lacking endogenous RB1 demonstrated that this amino acid change was sufficient to inhibit phosphorylation. In addition, this cysteine-to-phenylalanine substitution also resulted in loss of RB1 binding to the simian virus 40 large tumor and adenovirus E1A transforming proteins. These results confirm the importance of exon 21 coding sequences and suggest that the cysteine residue at codon 706 may play a role in achieving a specific protein conformation essential for ...

364

Fluoride induced changes in protein metabolism in the tissues of freshwater crab Barytelphusa guerini.

Electronic circular dichroism (ECD) of the n-#pi# and #pi# -#pi# transitions of the amide groups in the ultraviolet has become an indispensable tool for qualitative characterization of proteins in solution. Since the existence of such a spectrum (that is, of natural optical activity) comes from the three dimensional interaction of the chromophores in the molecule, CD is exquisitely sensitive to molecular conformation. However, in proteins, the amide transitions available in the near UV are limited in number and are broads and overlapping. Interactions among them yield information about the polymeric backbone, but since such amide electronic excitation are relatively delocalized, the resulting

1996-11-01

365

Cysteine-containing peptides having antioxidant properties

Exposure of the freshwater field crab Barytelphusa guerini to a sublethal concentration of NaF (30 mg litre(-1)) caused significant alterations in protein metabolism. After an exposure time of 15 days, the crab was found to have a marked depletion of total protein and free amino acid content. A significant elevation in transaminases, Aspartate aminotransferase (AAT) and Alanine aminotransferase (ALAT), and Glutamate dehydrogenase (GDH) activities was reflected in the free amino acid levels of the tissues. PMID:15092215

1990-01-01

366

Wnt/b-catenin signal pathway stabilizes APP intracellular domain (AICD) and promotes its transcriptional activity

The term "homology" or "homologous" means an amino acid similarity measured by the program, BLAST (Altschul et al (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:33 89 3402), and expressed as --(% identity n/n). In measuring homology between a peptide and a protein of greater size, homology is measured only in the corresponding region; that is, the protein is regarded as only having the same general length as the peptide, allowing for gaps and insertions.

2007-05-15

367

British Library Electronic Table of Contents (United Kingdom)

Amyloid precursor protein (APP), a key protein in pathogenesis of Alzheimer's disease (AD), is a type I transmembrane protein which can be cleaved by b- and g-secretase to release the amyloidogenic b-amyloid peptides (Ab) and the APP intracellular domain (AICD). While Ab has been widely believed to initiate pathogenic cascades culminating AD, the physiological functions and regulations of AICD remain elusive. In present study, endogenous AICD was demonstrated to be increased by canonical Wnt signal. Instead of due to g-secretase activity, enhanced AICD expression was found due to the increased protein stability by Wnt/b-catenin. b-Catenin was demonstrated to be an associating partner of AICD, capable of promoting AICD mediated transcriptional activity. Investigation by AICD mutants proved ...

2011-01-01

368

Tau inhibits tubulin oligomerization induced by prion protein

British Library Electronic Table of Contents (United Kingdom)

In previous studies we have demonstrated that prion protein (PrP) interacts with tubulin and disrupts microtubular cytoskeleton by inducing tubulin oligomerization. These observations may explain the molecular mechanism of toxicity of cytoplasmic PrP in transmissible spongiform encephalopathies (TSEs). Here, we check whether microtubule associated proteins (MAPs) that regulate microtubule stability, influence the PrP-induced oligomerization of tubulin. We show that tubulin preparations depleted of MAPs are more prone to oligomerization by PrP than those containing traces of MAPs. Tau protein, a major neuronal member of the MAPs family, reduces the effect of PrP. Importantly, phosphorylation of Tau abolishes its ability to affect the PrP-induced oligomerization of tubulin. We propose that t...

2011-01-01

369

Stochastic gene expression and its consequences

Site-Specific Methylation of the Promoter Alters Deoxyribonucleic Acid-Protein Interactions and Prevents ...

Gene expression is a fundamentally stochastic process, with randomness in transcription and translation leading to significant cell-to-cell variations in mRNA and protein levels. This variation...Full Text Available

2008-10-17

370

Refilling Intracellular Calcium Stores

... and R. L. Momparler. DNA methylation of retinoic acid receptor beta in breast cancer and possible therapeutic role of ... ...

371

Proteomic Analysis of Global Changes in Protein Expression During Exposure of Gamma Radiation in Bacillus sp. HKG 112 Isolated from Saline Soil

Within the cardiac cell, the movements of calcium ions are tightly regulated by a number of regulatory proteins including pumps, and channels. The sarcoplasmic reticulum (SR) is in large part...Full Text Available

2010-01-01

372

Protein expression profile from the Mesocestoides corti (Platyhelminthes: Cestoda) larval stage

Full Text Available

373

Pressure Stabilization of Proteins from Extreme Thermophiles

English 2007 [1 p.] Brazil Laschuk, A. Silveira, PA da Bizarro, CV Carvalho,

2007-05-21

374

Possible Implications of Placental Expressions for Heat Shock Protein 70 and Lectin-like Oxidized Low Density Lipoprotein Receptor-1 on Postnatal Blood Pressure in Their Newborns

We describe the stabilization by pressure of enzymes, including a hydrogenase from Methanococcus jannaschii, an extremely thermophilic deep-sea methanogen. This is the first published...Full Text Available

1994-03-01

375

Plasticity in patterns of histone modifications and chromosomal proteins in Drosophila heterochromatin

Full Text Available

2007-09-01

376

Phylogenomic analyses of malaria parasites and evolution of their exported proteins

Eukaryotic genomes are packaged in two basic forms, euchromatin and heterochromatin. We have examined the composition and organization of Drosophila melanogaster heterochromatin in...Full Text Available

2011-02-01

377

Phosphorylation of Annexin A1 by TRPM7 Kinase: A Switch Regulating the Induction of an ?-Helix

BackgroundPlasmodium falciparum is the most malignant agent of human malaria. It belongs to the taxon Laverania, which includes other ape-infecting Plasmodium...Full Text Available

378

PUTATIVE CSF PROTEIN BIOMARKER CANDIDATES FOR AMNESTIC MILD COGNITIVE IMPAIRMENT

TRPM7 is an unusual bifunctional protein consisting of an α-kinase...Full Text Available

2011-03-29

379

PHENOTYPIC MODIFICATION OF ROACH (RUTILUS RUTILUS L.) INFECTED WITH LIGULA INTESTINALIS L. (CESTODA: PSEUDOPHYLLIDEA)

The identification of individuals at risk for Alzheimer's disease (AD) is essential for the timely administration of treatment approaches aimed at slowing the onset or progression of the disease....Full Text Available

2010-03-01

380

Ovine reference materials and assays for prion genetic testing

... growth hormone-like factor from plerocercoids of the tapeworm Spirometra mansonoides is a multifunctional protein. In Parasites and pathogens: ... ...

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381

BackgroundGenetic predisposition to scrapie in sheep is associated with several variations in the peptide sequence of the prion protein gene (PRNP). DNA-based tests...Full Text Available

382

Orphan GPCR research

Mutations in RNA Binding Protein Gene Cause Familial Dilated Cardiomyopathy

Orphan G protein-coupled receptors (GPCRs) are receptors lacking endogenous ligands. Found by molecular biological analyses, they became the roots of reverse pharmacology, in which receptors are attempted...Full Text Available

2008-03-01

383

Liver Carcinogenesis Unit

ObjectivesWe sought to identify a novel gene for dilated cardiomyopathy (DCM).BackgroundDCM is a heritable, genetically...Full Text Available

2009-09-01

384

Inhibition of heat shock protein 27 (HspB1) tumorigenic functions by peptide aptamers

The Liver Carcinogenesis Section uses the modern technologies of molecular biology, cell biology, protein chemistry and genetics to investigate the biochemical and genetic pathways involved in human liver cancer.

385

British Library Electronic Table of Contents (United Kingdom)

Human heat shock protein 27 (Hsp27, HspB1) is an anti-apoptotic protein characterized for its tumorigenic and metastatic properties, and now referenced as a major therapeutic target in many types of cancer. Hsp27 biochemical properties rely on a structural oligomeric and dynamic organization. Downregulation by small interfering RNA or inhibition with dominant-negative mutant have proven their efficiency to counteract the anti-apoptotic and protective properties of Hsp27. In this study, we report the isolation and characterization of Hsp27-targeted molecules interfering with its structural organization. Using the peptide aptamer (PA) strategy, we isolated PAs that specifically interact with Hsp27 and not with the other members of the small heat shock protein family. In mammalian cell cultur...

2011-01-01

386

Influence of KDEL on the Fate of Trimeric or Assembly-Defective Phaseolin

Identification of Contractile Vacuole Proteins in Trypanosoma cruzi

The tetrapeptide KDEL is commonly found at the C terminus of soluble proteins of the endoplasmic reticulum (ER), and it contributes to their localization by interacting with a receptor that recycles...Full Text Available

2001-05-01

387

Hydrophobic labelling of membrane-embedded proteins with lipophilic reagents

Contractile vacuole complexes are critical components of cell volume regulation and have been shown to have other functional roles in several free-living protists....Full Text Available

388

High-affinity lead binding proteins in rat kidney cytosol mediate cell-free nuclear translocation of lead

Hydrophobic labelling is frequently used in the study of membrane-inserted domains of intrinsic proteins. However, the published procedures, fail to incorporate sufficient radioactivity into membrane immunoglobulins of B lymhocytes to permit investigation of their subunit structures and associations with other proteins. In order to increase the specific radioactivity of ["1"2"5I]iodonaphtylazide ["1"2"5I]INA), an improved method for the synthesis of the reagent was developed. In addition, the optimal conditions for labelling B lymhpocytes with ["1"2"5I]INA and commercially available reagent 3-(trifluoromethyl)-3-(trifluoromethyl)-3-(3'-["1"2"5Iliodophenyl)diazirine (["1"2"5I]TID were isolated and analysed in detail by SDS-PAGE. The usefulness of the two reagents for the investigation of lipid-embedded domains of membrane proteins is discussed. (author). 28 refs.; 4 figs.

389

High-affinity lead binding proteins in rat kidney cytosol mediate cell-free nuclear translocation of lead

The PbII binding characteristics of the previously reported PbII binding proteins of rat kidney cytosol were investigated further. Saturation and Scatchard analysis of /sup 203/Pb binding in whole cytosol and in 40% saturated ammonium sulfate precipitated fractions disclosed a class of relatively high-affinity sites with an apparent Kd of approximately 50 nM and binding capacities of approximately 41 and 9 pmol/mg of protein, respectively. Two /sup 203/Pb binding proteins with approximate molecular masses of 63K and 11.5K daltons and a high molecular weight component (greater than 200K) were isolated by Sepharose-6B column chromatography. The time course of association of /sup 203/Pb with cytosol and the 63K protein showed maximum binding at 18 hr which was stable up to 25 hr at 4 degrees C. The approximate half-time dissociation rate (T 1/2) of specifically bound /sup 203/Pb to the 63K ...

1985-02-01

390

HDL in humans with cardiovascular disease exhibits a proteomic signature

The PbII binding characteristics of the previously reported PbII binding proteins of rat kidney cytosol were investigated further. Saturation and Scatchard analysis of "2"0"3Pb binding in whole cytosol and in 40% saturated ammonium sulfate precipitated fractions disclosed a class of relatively high-affinity sites with an apparent Kd of approximately 50 nM and binding capacities of approximately 41 and 9 pmol/mg of protein, respectively. Two "2"0"3Pb binding proteins with approximate molecular masses of 63K and 11.5K daltons and a high molecular weight component (greater than 200K) were isolated by Sepharose-6B column chromatography. The time course of association of "2"0"3Pb with cytosol and the 63K protein showed maximum binding at 18 hr which was stable up to 25 hr at 4 degrees C. The approximate half-time dissociation rate (T 1/2) of specifically bound "2"0"3Pb to the 63K protein ...

391

Favism, with special reference to Iran*

BackgroundAlterations in protein composition and oxidative damage of high-density lipoprotein (HDL) have been proposed to impair the cardioprotective properties of...Full Text Available

2010-07-04

392

Evaluation of the effect of Maytenus ilicifolia on the labelling of red blood cells and plasma proteins with Technetium-99m

Fava beans (Vicia fava) are cultivated rather widely in most countries of the Eastern Mediterranean area and provide a cheap but protein-rich food that can be eaten alone, in various...Full Text Available

1969-01-01

393

Evaluating concentration estimation errors in ELISA microarray experiments

English 1998 p. 196 Brazil Oliveira, JF Braga, ACS Santos, JS Bernardo Filho,

1998-05-23

394

Diagnostic Value of C-reactive Protein in Complicated Appendicitis

BackgroundEnzyme-linked immunosorbent assay (ELISA) is a standard immunoassay to estimate a protein's concentration in a sample. Deploying ELISA in a microarray format permits simultaneous...Full Text Available

395

Degradation of leucine zipper-positive isoform of MYPT1 may contribute to development of nitrate tolerance

PurposeEarly detection of appendicitis has increased due to development of computed tomography and ultrasonography, yet we are frequently meeting complicated appendicitis, including...Full Text Available

2011-06-01

396

British Library Electronic Table of Contents (United Kingdom)

Aims A depressed cGMP-dependent protein kinase (PKG) activity is implicated in nitrate tolerance. The present study determines whether the leucine zipper-positive (LZ+) isoform of myosin phosphatase target subunit 1 (MYPT1), a key target protein for PKG actions, is involved in the development of nitrate tolerance. Methods and results Nitrate tolerance in in vitro preparations was obtained by a 24 h incubation with nitroglycerin (NTG). Nitrate tolerance in in vivo preparations was obtained by subcutaneous injection of mice with NTG, and the aortas were used. Protein levels of total MYPT1, MYPT1 (LZ+), PP1Cd, myosin light chain (MLC), and phosphorylated MLC were determined by Western blot analysis. Isometric vessel tension was determined by an organ chamber technique. Protein levels of MYPT1...

2010-01-01

397

Crystal structure of outer surface protein C (OspC) from the Lyme disease spirochete, Borrelia burgdorferi

Comparative study of functional properties of commercial and membrane processed yellow pea protein isolates

English 21 Jan 2001 [10 p.] United Kingdom Kumaran, D Eswaramoorthy,

2001-01-21

398

British Library Electronic Table of Contents (United Kingdom)

Functional properties of commercial and membrane processed pea protein isolates (PPI) prepared from yellow peas were investigated. Four protein isolates were prepared from yellow pea flour using water and KCl extractions at 25^oC followed by ultrafiltration and diafiltration (UF and DF) at pHs of 7.5 and 7.5 or 6 respectively. Following assessment of compositional attributes; solubility, foaming, flow and dynamic rheology, emulsification ability and heat-induced textural and rheological properties of prepared PPIs and a commercially available PPI were tested and compared. Membrane purification of proteins resulted in 28% to 68% reduction in phytic acid and enhanced, comparatively, the tested functional properties. Solubility of membrane processed PPIs, at all tested pHs, was superior and t...

2011-01-01

399

Clonidine, octopaminergic receptor agonist, reduces protein feeding in the blow fly, Phormia regina (Meigen)

British Library Electronic Table of Contents (United Kingdom)

Results in this study are consistent with those of Murdock and his colleagues who clearly demonstrated that clonidine, an agonist of octopaminergic receptors in some insects, significantly increases sucrose feeding. Their studies, however, did not examine the effect of clonidine on protein feeding. Injection of a 20mg/ml/fly dose of clonidine significantly reduces protein feeding in both sexes of Phormia regina, instead of stimulating feeding as is observed with carbohydrate feeding. The manner in which the flies are fed prior to starvation and the method of testing influences the amounts of diet consumed. It is proposed that the biogenic amines influence the state of hunger (i.e., protein versus carbohydrates) while other chemicals and neural mechanisms (i.e., such as sulfakinins and stre...

2007-01-01

400

Carbohydrate-specified endocytosis: localization of ligand in the lysosomal compartment.

BzpF is a CREB-like transcription factor that regulates spore maturation and stability in Dictyostelium

Carbohydrate-directed endocytosis is mediated by a receptor, the hepatic binding protein; it is responsible for the clearance of galactose-terminated glycoproteins from the circulation. This process...Full Text Available

1981-11-01

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401

British Library Electronic Table of Contents (United Kingdom)

The cAMP response element-binding protein (CREB) is a highly conserved transcription factor that integrates signaling through the cAMP-dependent protein kinase A (PKA) in many eukaryotes. PKA plays a critical role in Dictyostelium development but no CREB homologue has been identified in this system. Here we show that Dictyostelium utilizes a CREB-like protein, BzpF, to integrate PKA signaling during late development. bzpF^- mutants produce compromised spores, which are extremely unstable and germination defective. Previously, we have found that BzpF binds the canonical CRE motif in vitro. In this paper, we determined the DNA binding specificity of BzpF using protein binding microarray (PBM) and showed that the motif with the highest specificity is a CRE-like sequence. BzpF is necessary to ...

2011-01-01

402

Avoidance of hydrolyzed casein by mice

Association and expression study of synapsin III and schizophrenia

When casein, a milk protein, is hydrolyzed, it renders human foods that contain it (e.g., hypoallergenic infant formula, cheeses) distasteful to many people. This rejection...Full Text Available

2008-01-28

403

Application of laser fluorimetry for determining the influence of a single amino-acid substitution on the individual photophysical parameters of a fluorescent form of a fluorescent protein mRFP1

The synapsin III gene, SYN3, which belongs to the family of synaptic vesicle-associated proteins, has been implicated in the modulation of neurotransmitter...Full Text Available

2009-11-20

404

Alpha-toxin of Staphylococcus aureus.

Individual photophysical parameters of the chromophore of a fluorescent protein mRFP1 and its two mutants (amino-acid substitution at position 66 - mRFP1/ Q66C and mRFP1/Q66S proteins) are determined. For this purpose, apart from conventional methods of fluorimetry and spectrophotometry, nonlinear laser fluorimetry is used. It is shown that the individual extinction coefficients of the chromophore of proteins correlate (correlation coefficient above 0.9) with the volume of the substituted amino-acid residue at position 66 (similar to the positions of the absorption, fluorescence excitation and emission maxima). (laser biology and medicine)

2009-03-31

405

Adducted proteins for identification of endogenous electrophiles.

Alpha-toxin, the major cytotoxic agent elaborated by Staphylococcus aureus, was the first bacterial exotoxin to be identified as a pore former. The protein is secreted as a single-chain, water-soluble...Full Text Available

1991-12-01

406

AEC syndrome - Genetics Home Reference

Chemically reactive compounds in tissues can be monitored through their products of reaction with biomacromolecules. For the purpose of in vivo dose monitoring, hemoglobin (Hb) has been preferred to...Full Text Available

1993-03-01

407

A comprehensive assessment of N-terminal signal peptides prediction methods

What genes are related to AEC syndrome? AEC syndrome is caused by mutations in the TP63 gene. This gene provides instructions for making a protein known as p63, which plays an...

2011-10-15

408

0100127 - Liquid nitrogen dewar for protein crystal growth - NASA

BackgroundAmino-terminal signal peptides (SPs) are short regions that guide the targeting of secretory proteins to the correct subcellular compartments in the cell. They are cleaved...Full Text Available

409

Yeast ribosomal protein L1 is required for the stability of newly synthesized 5S rRNA and the assembly of 60S ribosomal subunits.

Gaseous Nitrogen Dewar apparatus developed by Dr. Alex McPherson of the University of California, Irvine for use aboard Mir and the International Space ...

410

Uteroglobin gene expression in the rabbit uterus throughout gestation and in the fetal lung. Relationship between uteroglobin and eicosanoid levels in the developing fetal lung.

Ribosomal protein L1 from Saccharomyces cerevisiae binds 5S rRNA and can be released from intact 60S ribosomal subunits as an L1-5S ribonucleoprotein (RNP) particle. To understand the nature of the...Full Text Available

1993-05-01

411

The molybdenum iron-sulphur protein from Desulfovibrio gigas as a form of aldehyde oxidase.

Uteroglobin (UG) gene encodes a cytokine-like, multifunctional, antiinflammatory protein, with potent phospholipase A2-inhibitory activity. It has been suggested that during implantation this protein...Full Text Available

1995-07-01

412

The human U1-70K snRNP protein: cDNA cloning, chromosomal localization, expression, alternative splicing and RNA-binding.

The molybdenum iron-sulphur protein originally isolated from Desulfovibrio gigas by Moura, Xavier, Bruschi, Le Gall, Hall & Cammack [(1976) Biochem. Biophys. Res. Commun. 72, 782-789] has been...Full Text Available

1987-05-01

413

The holographic principle and the language of genes

We have isolated and sequenced cDNA clones encoding the human U1-70K snRNP protein, and have mapped this locus (U1AP1) to human chromosome 19. The gene produces two size classes of RNA, a major 1.7-kb...Full Text Available

1987-12-23

414

The direct interaction between ASH2, a Drosophila trithorax group protein, and SKTL, a nuclear phosphatidylinositol 4-phosphate 5-kinase, implies a role for phosphatidylinositol 4,5-bisphosphate in maintaining transcriptionally active chromatin.

We show that the holographic principle in quantum gravity imposes a strong constraint on life. The degrees of freedom of an organism can be estimated according to the theory of Boolean networks, which is constrained by the entropy bound. Hence we can explain the languages in protein sequences or in DNA sequences. The overall evolution of biological complexity can be illustrated. And some general properties of protein length distributions can be explained by a linguistic mechanism.

2008-01-01

415

The Membrane Bound LRR Lipoprotein Slr, and the Cell Wall-Anchored M1 Protein from Streptococcus pyogenes Both Interact with Type I Collagen

The products of trithorax group (trxG) genes maintain active transcription of many important developmental regulatory genes, including homeotic genes. Several trxG proteins have been shown to act in...Full Text Available

2004-07-01

416

The Function of Hypoxia-Inducible Factor (HIF) Is Independent of the Endoplasmic Reticulum Protein OS-9

Streptococcus pyogenes is an important human pathogen and surface structures allow it to adhere to, colonize and invade the human host. Proteins containing leucine rich repeats (LRR)...Full Text Available

417

Temporal patterns of protein phosphorylation after angiotensin II, A23187 and/or 12-O-tetradecanoylphorbol 13-acetate in adrenal glomerulosa cells.

The protein “amplified in osteosarcoma-9” (OS-9) has been shown previously to interact with the prolyl hydroxylases PHD2 and PHD3. These enzymes initiate oxygen-dependent degradation...Full Text Available

418

Synthesis and cloning of the genes of antisense peptides of human calcitonin and miniproinsulin

The temporal patterns of protein phosphorylation in the adrenal glomerulosa cell were analysed by two-dimensional electrophoresis after stimulation with 10 nM-angiotensin II or various agents [10 nM-12-O-tetradecanoylphorbol...Full Text Available

1986-09-15

419

Structural features underlying selective inhibition of protein kinase CK2 by ATP site-directed tetrabromo-2-benzotriazole

With the aim of an experimental check on the validity of the theory of molecular recognition, the authors have carried out the chemical-enzymatic synthesis and cloning of the gene of human calcitonin and also of the genes of antisense polypeptides to human calcitonin and miniproinsulin. It has been shown that recombinant plasmids obtained on the basis of these synthetic genes are capable of ensuring the biosynthesis of the given polypeptides in E. coli cells as hybrid proteins with the IgG-binding domain of staphylococcal protein A.

1994-07-20

420

Structural Chemistry of Human SET Domain Protein Methyltransferases

Two novel crystal structures of Zea mays protein kinase CK2α catalytic subunit, one in complex with the specific inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) and another...Full Text Available

2001-11-01

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421

Sequence analysis of two alleles reveals that intra-and intergenic recombination played a role in the evolution of the radish fertility restorer (Rfo)

There are about fifty SET domain protein methyltransferases (PMTs) in the human genome, that transfer a methyl group from S-adenosyl-L-methionine (SAM) to substrate lysines on histone tails or other peptides. A number of structures in complex with cofactor, substrate, or inhibitors revealed the mechanisms of substrate recognition, methylation state specificity, and chemical inhibition. Based on these structures, we review the structural chemistry of SET domain PMTs, and propose general concepts towards the development of selective inhibitors.

2011-08-22

422

SAS1 and SAS2, GTP-binding protein genes in Dictyostelium discoideum with sequence similarities to essential genes in Saccharomyces cerevisiae.

BackgroundLand plant genomes contain multiple members of a eukaryote-specific gene family encoding proteins with pentatricopeptide repeat (PPR) motifs. Some PPR proteins were shown...Full Text Available

423

Roles of the TNF receptor-associated factor (TRAF)2/3 binding site in differential B cell signaling by CD40 and its viral oncogenic mimic, latent membrane protein 1 (LMP1)

We have identified two novel, very closely related genes, SAS1 and SAS2, from Dictyostelium discoideum. These encode small, approximately 20-kilodaton proteins with amino acid sequences thought to be...Full Text Available

1990-05-01

424

Repetitive satellite-like sequences are present within or upstream from 3 avian protein-coding genes.

The Epstein-Barr virus protein, LMP1, is a functional mimic of the cellular receptor CD40, but signals to B lymphocytes in an amplified and sustained manner compared to CD40. LMP1 contributes...Full Text Available

2009-09-01

425

Relative Rates of Transcapillary Movement of Free Thyroxine, Protein-Bound Thyroxine, Thyroxine-Binding Proteins, and Albumin

Peculiar DNA sequences made up by the tandem repetition of a 5 bp unit have been identified within or upstream from three avian protein-coding genes. One sequence is located within an intron of the...Full Text Available

1983-03-11

426

Reducing Rice Seed Storage Protein Accumulation Leads to Changes in Nutrient Quality and Storage Organelle Formation¹^[W]^[OA]

The rate of appearance of labeled thyroxine (T4) and albumin in lymph from various areas after simultaneous i.v. injection of the labeled substances in conscious ambulatory sheep has been used to estimate...Full Text Available

1974-07-01

427

RecA protein acts at the initiation of stable DNA replication in rnh mutants of Escherichia coli K-12.

Rice (Oryza sativa) seed storage proteins (SSPs) are synthesized and deposited in storage organelles in the endosperm during seed maturation as a nitrogen source for germinating seedlings....Full Text Available

2010-12-01

428

Protein oxidation markers in women with and without gestational diabetes mellitus: A possible relation with paraoxonase activity.

Escherichia coli rnh mutants lacking RNase H activity are capable of recA+-dependent DNA replication in the absence of concomitant protein synthesis (stable DNA replication). In rnh dnaA::Tn10 and rnh...Full Text Available

1985-08-01

429

Protein dynamics. Vibrational coupling, spectral broadening mechanisms, and anharmonicity effects in carbonmonoxy heme proteins studied by the temperature dependence of the Soret band lineshape.

AIMS: To clarify the levels of protein oxidation markers such as protein carbonyl (PCO), protein hydroperoxides (P-OOH), advanced oxidation protein products (AOPP) and nitrotyrosine (NT), as well as antioxidative enzymes such as paraoxonase (PON-1) in women with and without gestational diabetes mellitus (GDM). METHODS: The study was conducted on 23 women with GDM and 22 women without GDM. The levels of the P-OOH, AOPP, and PON-1 were determined by colorimetric methods; whereas NT and PCO levels were measured by ELISA. RESULTS: The concentrations of protein oxidation markers were significantly increased and PON1 activity was significantly decreased in GDM group compared to those of normal pregnant women. The control group showed a significant negative correlation between PON-1 and PCO (r=-0.451, p=0.027); whereas in GDM group, there was a significant positive correlation between ...

2011-08-30

430

Primary structure of streptococcal Pep M5 protein: Absence of extensive sequence repeats

In this work we study the temperature dependence of the Soret band lineshape of the carbonmonoxy derivatives of sperm whale myoglobin, human hemoglobin, and its isolated alpha and beta subunits. To...Full Text Available

1992-08-01

431

Position-Dependent Electrostatic Protection against Protein Aggregation

Extensive sequence repeats have been observed in a biologically active fragment of type 24 streptococcal M protein, namely Pep M24 [Beachey, E. H., Sayer, J. M. & Kang, A. H. (1978) Proc....Full Text Available

1983-09-01

432

British Library Electronic Table of Contents (United Kingdom)

Proteins with a high propensity to aggregate can be largely prevented from doing so with surprisingly small changes to their primary structure. By using a combination of rational design and quantitative measurements of aggregation rates, we show that adding a single charge in specific "gatekeeper" regions is sufficient to change the timescale for amyloid fibril growth from minutes to weeks, thereby dramatically reducing the efficiency of this process.

2009-01-01

433

Polyunsaturated Fatty Acids Selectively Suppress Sterol Regulatory Element-binding Protein-1 through Proteolytic Processing and Autoloop Regulatory Circuit*

Phosphorylation of ?6-Tubulin by Protein Kinase C? Activates Motility of Human Breast Cells*

Sterol regulatory element-binding protein (SREBP)-1 is a key transcription factor for the regulation of lipogenic enzyme genes in the liver. Polyunsaturated fatty acids (PUFA) selectively suppress hepatic...Full Text Available

2010-04-09

434

Outcome predictability of biomarkers of protein-energy wasting and inflammation in moderate and advanced chronic kidney disease¹²³⁴

Engineered overexpression of protein kinase Cα (PKCα) was previously shown to endow nonmotile MCF-10A human breast cells with aggressive motility. A traceable mutant of PKCα...Full Text Available

2009-06-26

435

On the Origins of the Weak Folding Cooperativity of a Designed ??? Ultrafast Protein FSD-1

Background: Markers of protein-energy wasting (PEW) and inflammation are common in chronic kidney disease (CKD) and are among the strongest predictors of mortality in dialysis patients.Objective:...Full Text Available

2009-08-01

436

FSD-1, a designed small ultrafast folder with a ββα fold, has been actively studied in the last few years as a model system for studying protein folding mechanisms and for testing...Full Text Available

2010-11-01

437

Mutations Affecting Motifs of Unknown Function in the Central Domain of Nitrogen Regulatory Protein C

BackgroundUsing a transcriptional profiling approach, we recently identified myeloid-related protein-8/14 (MRP-8/14) to be expressed by platelets during acute MI....Full Text Available

2008-01-01

438

Mice With a Deletion in the Gene for CCAAT/Enhancer-Binding Protein ? are Protected Against Diet-Induced Obesity

The positive control function of the bacterial enhancer-binding protein NtrC resides in its central domain, which is highly conserved among activators of ς⁵⁴ holoenzyme. Previous...Full Text Available

1999-09-01

439

Method for detecting and diagnosing disease caused by pathological protein aggregation

The CCAAT/enhancer-binding protein β (C/EBPβ) is required for adipocyte differentiation and maturation. We have studied the role of the transcription factor, C/EBPβ,...Full Text Available

2007-01-01

440

Kes1p shares homology with human oxysterol binding protein and participates in a novel regulatory pathway for yeast Golgi-derived transport vesicle biogenesis.

A method is provided for detecting pathological macromolecules in a patient. The method is comprised of the following: obtaining body fluid from the patient; pretreating the body fluid; subjecting the pretreated body fluid to size-exclusion chromatography to create an excluded fluid; and analyzing the excluded fluid to detect macromolecules having a predetermined molecular weight. The method also allows for comparing elution spectra with reference spectra of suspect pathologic proteins.

2000-05-16

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

441

Involvement of stress-activated protein kinase and p38 mitogen-activated protein kinase in mIgM-induced apoptosis of human B lymphocytes

The yeast phosphatidylinositol transfer protein (Sec14p) is required for biogenesis of Golgi-derived transport vesicles and cell viability, and this essential Sec14p requirement is abrogated by inactivation...Full Text Available

1996-12-02

442

Immunohistochemical localization of low density lipoprotein receptor-related protein 1 and ?₂-Macroglobulin in retinal and choroidal tissue of proliferative retinopathies

Despite intensive efforts, the intracellular signaling pathways that mediate apoptosis remain unclear. The human B lymphoma cell line, B104, possesses characteristics that make it an attractive model...Full Text Available

1996-11-26

443

Hyperphosphorylation of the Rotavirus NSP5 Protein Is Independent of Serine 67 or NSP2, and the Intrinsic Insolubility of NSP5 Is Regulated by Cellular Phosphatases

The immunolocalization of the low density lipoprotein receptor-related protein 1 (LRP1) and its ligand alpha 2-Macroglobulin (α₂M) was examined in tissues from human donor...Full Text Available

2010-08-01

444

HupUV proteins of Rhodobacter capsulatus can bind H2: evidence from the H-D exchange reaction.

The NSP5 protein is required for viroplasm formation during rotavirus infection and is hyperphosphorylated into 32- to 35-kDa isoforms. Earlier studies reported that NSP5 is not hyperphosphorylated...Full Text Available

2006-02-01

445

High throughput protein fold identification by using experimental constraints derived from intramolecular cross-links and mass spectrometry

The H-D exchange reaction has been measured with the D2-H2O system, for Rhodobacter capsulatus JP91, which lacks the hupSL-encoded hydrogenase, and R. capsulatus BSE16, which lacks the HupUV proteins....Full Text Available

1997-01-01

446

Hepatitis C Virus RNA Replication Requires a Conserved Structural Motif within the Transmembrane Domain of the NS5B RNA-Dependent RNA Polymerase ?

We have used intramolecular cross-linking, MS, and sequence threading to rapidly identify the fold of a model protein, bovine basic fibroblast growth factor (FGF)-2. Its tertiary structure was probed...Full Text Available

2000-05-23

447

Helper T-Cell Epitopes Encoded by the Babesia bigemina rap-1 Gene Family in the Constant and Variant Domains Are Conserved among Parasite Strains

Hepatitis C virus (HCV) nonstructural protein 5B (NS5B), the viral RNA-dependent RNA polymerase (RdRp), is a tail-anchored protein with a highly conserved C-terminal transmembrane domain (TMD) that...Full Text Available

2010-11-01

448

External reflection absorption infrared spectroscopy study of lung surfactant proteins SP-B and SP-C in phospholipid monolayers at the air/water interface.

Among important candidates for babesial vaccines are apical complex proteins, including rhoptry-associated protein 1 (RAP-1) from Babesia bovis and B. bigemina, which...Full Text Available

1998-04-01

449

Exploiting the beneficial effects of PPO on the utilisation of protein and lipids in grazed forages

The interactions of the hydrophobic pulmonary surfactant proteins SP-B and SP-C with 1,2-dipalmitoylphosphatidylcholine in mixed, spread monolayer films have been studied in situ at the air/water interface...Full Text Available

1995-12-01

450

Environmental Research Database

DescriptionThe plant enzyme polyphenol oxidase (PPO) has been demonstrated to reduce both proteolysis and lipolysis during the ensiling of red clover. The purpose of this work is to explore the potential for exploiting this beneficial trait in other dietary regimes for grazing ruminants. These studies will focus on the development of new strategies for forage management and livestock feeding by increasing the efficiency of feed protein-N utilisation and reducing saturated fatty acid production by ruminan [continued...

2007-01-31

451

Evaporator condensates: continuous bioprocessing of simulated sulfite condensates to produce single cell protein. [Candida utilis

Ebola Virus VP40-Induced Particle Formation and Association with the Lipid Bilayer

The continuous bioprocessing of simulated steam-stripped sulfite pulp mill evaporator condensates using Candida utilis to produce single cell protein has been studied. At 32 degrees C, processing can be conducted at space velocities of up to 0.43 hr/sup -1/ with over 90% removal of acetic acid present at an initial concentration of 2 g/liter. (Refs. 16).

1982-11-01

452

Differential Changes in Heat Shock Protein-, Lipoarabinomannan-, and Purified Protein Derivative-Specific Immunoglobulin G1 and G2 Isotype Responses during Bovine Mycobacterium avium subsp. paratuberculosis Infection

Viral protein 40 (VP40) of Ebola virus appears equivalent to matrix proteins of other viruses, yet little is known about its role in the viral life cycle. To elucidate the functions of VP40, we investigated...Full Text Available

2001-06-01

453

Determination by enzyme-linked immunosorbent assay of immunoglobulin G (IgG), IgM, and IgA to Brucella melitensis major outer membrane proteins and whole-cell heat-killed antigens in sera of patients with brucellosis.

Bovine paratuberculosis is caused by infection of young calves with Mycobacterium avium subsp. paratuberculosis. In some of the chronically infected cows the long asymptomatic...Full Text Available

2001-03-01

454

Decreased serum protein binding of diazepam and its major metabolite in the neonate during the first postnatal week relate to increased free fatty acid levels.

An enzyme-linked immunosorbent assay was used to compare Brucella melitensis major outer membrane proteins (MOMP) and whole-cell heat-killed antigens (HK) in measuring antibrucella immunoglobulin G...Full Text Available

1989-08-01

455

Decreased binding of drugs and dyes to plasma proteins from rats with acute renal failure: effects of ureter ligation and intramuscular injection of glycerol.

The protein binding of diazepam (D) and its major active metabolite N-desmethyl diazepam (DD) was investigated in vitro in the serum of 14 mothers at birth, 21 foetuses at birth, in 100 neonates between...Full Text Available

1984-01-01

456

1 The decreased binding of drugs and dyes to plasma proteins from male and female rats with acute renal failure has been investigated using equilibrium dialysis at 37 degrees C. 2 Acute renal failure...Full Text Available

1979-06-01

457

DNA repair genes

DIMINISHED DEGRADATION OF MYELIN BASIC PROTEIN BY ANTI-SULFATIDE ANTIBODY AND INTERFERON-? IN MYELIN FROM GLIA MATURATION FACTOR-DEFICIENT MICE

Fission yeast S. pombe is assumed to be a good model for cloning of human DNA repair genes, because human gene is normally expressed in S. pombe and has a very similar protein sequence to yeast protein. We have tried to elucidate the DNA repair mechanisms of S. pombe as a model system for those of mammals. (J.P.N.)

1995-12-01

458

Crystal structures of the N-terminal kinase domain of human RSK1 bound to three different ligands: Implications for the design of RSK1 specific inhibitors

In this study we show the effect of anti-sulfatide (RmAb) antibodies and inflammatory cytokines, TNF-α and IFN-γ in inducing myelin basic protein (MBP) degradation in myelin...Full Text Available

2007-06-01

459

Characterization of the major phosphoprotein and its kinase on the surface of the rat adipocyte

The p90 ribosomal S6 kinases (RSKs) also known as MAPKAP-Ks are serine/threonine protein kinases that are activated by ERK or PDK1 and act as downstream effectors of mitogen-activated protein kinase...Full Text Available

2007-12-01

460

Binding the Mammalian High Mobility Group Protein AT-hook 2 to AT-Rich Deoxyoligonucleotides: Enthalpy-Entropy Compensation

Intact rat fat cell exposed to 12.5 ..mu..M (..gamma..-32P)ATP incorporate label into specific proteins within minutes. By solubilizing the reaction mixture with SDS which bypasses the subcellular fractionation steps, the labeled proteins can be identified in autoradiographs of SDS-PAGE gels. The most prominently labeled protein has an M/sub r/ of 42,000. Localization of this component to the cell surface can be made on the basis of inhibition of phosphorylation by addition of a protein derived from the rat brain with protein kinase inhibitory property, susceptibility of the phosphorylated protein to the tryptic digestion, inhibition of phosphorylation of this protein after brief exposure to melittin. To rule out the possibility that the cell surface protein might be a mitochondrial contaminant from broken cells, /sup ...

1986-12-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

461

Basis for the Specificity and Activation of the Serpin Protein Z-dependent Proteinase Inhibitor (ZPI) as an Inhibitor of Membrane-associated Factor Xa*

HMGA2 is a DNA minor-groove binding protein. We previously demonstrated that HMGA2 binds to AT-rich DNA with very high binding affinity where the binding of HMGA2 to poly(dA-dT)₂ is enthalpy-driven...Full Text Available

2009-05-20

462

Association between glucokinase regulatory protein (GCKR) and apolipoprotein A5 (APOA5) gene polymorphisms and triacylglycerol concentrations in fasting, postprandial, and fenofibrate-treated states¹²³

The serpin ZPI is a protein Z (PZ)-dependent specific inhibitor of membrane-associated factor Xa (fXa) despite having an unfavorable P1 Tyr. PZ accelerates the inhibition reaction ∼2000-fold...Full Text Available

2010-06-25

463

Assembly of 60S ribosomal subunits is perturbed in temperature-sensitive yeast mutants defective in ribosomal protein L16.

Background: Hypertriglyceridemia is a risk factor for cardiovascular disease. Variation in the apolipoprotein A5 (APOA5) and glucokinase regulatory protein (GCKR)...Full Text Available

2009-01-01

464

Amino acid sequences that determine the nuclear localization of yeast histone 2B.

Temperature-sensitive mutants defective in 60S ribosomal subunit protein L16 of Saccharomyces cerevisiae were isolated through hydroxylamine mutagenesis of the RPL16B gene and plasmid shuffling. Two...Full Text Available

1991-11-01

465

Affinity labelling of ribosomes from the livers of different vertebrates by 2-nitro-4-azidobenzoyl-Phe-tRNA

Histone-beta-galactosidase protein fusions were used to identify the domain of yeast histone 2B, which targets this protein to the nucleus. Amino acids 28 to 33 in H2B were required for nuclear localization...Full Text Available

1987-11-01

466

A region in the cytosolic domain of the epidermal growth factor receptor antithetically regulates the stimulatory and inhibitory guanine nucleotide-binding regulatory proteins of adenylyl cyclase.

Ribosomal protein L 10 from the livers of trout, hen, and rat was found to be the main target for 2-nitro-4-azidobenzoyl-Phe-tRNA in affinity labelling experiments. Therefore, despite somewhat different electrophoretic mobilities, this protein seems to be involved in the organization of the peptidyl transferase centre in ribosomes of various vertebrates. (author).

467

5'-Azido-[3,6-3H2]-1-napthylphthalamic acid, a photoactivatable probe for naphthylphthalamic acid receptor proteins from higher plants: identification of a 23-kDa protein from maize coleoptile plasma membranes.

Epidermal growth factor (EGF) stimulates adenylyl cyclase in the heart via activation of the stimulatory GTP-binding protein Gs. Therefore, employing peptides corresponding to regions in the cytosolic...Full Text Available

1995-03-14

468

Unusual properties of crocodilian ovomacroglobulin shown in its methylamine treatment and sulfhydryl titration

1-Naphthylphthalamic acid (NPA) is a specific inhibitor of polar auxin transport that blocks carrier-mediated auxin efflux from plant cells. To allow identification of the NPA receptor thought to be...Full Text Available

1992-01-15

469

Trans-activation of the JC virus late promoter by the tat protein of type 1 human immunodeficiency virus in glial cells

The inhibitory activity of chicken and crocodilian ovomacroglobulins against trypsin was measured before and after their incubation with methylamine. The result for crocodilian ovomacroglobulin showed that methylamine treatment destroyed half of its activity, in unique contrast to human alpha 2-macroglobulin and chicken ovomacroglobulin for which methylamine either destroys the inhibitory activity of the former completely or does not affect that of the latter at all. Free sulfhydryl groups of chicken and crocodilian ovomacroglobulins were titrated with 5,5'-dithiobis(2-nitrobenzoic acid) before and after incubation with trypsin. Prior to the incubation with trypsin the chicken and crocodilian proteins respectively had 0 and 1 titratable sulfhydryl per molecule of Mr 720,000. After treatment with trypsin the crocodilian protein had 3.5-4 titratable sulfhydryls, whereas there were no titratable sulfhydryls in the chicken ...

1986-02-01

470

Studies on metabolism of directly labeled {sup 99}MTc-antibody in mice

Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central nervous system caused by the JC virus (JCV), a human papovavirus. PML is a relatively rare disease seen predominantly in immunocompromised individuals and is a frequent complication observed in AIDS patients. The significantly higher incidence of PML in AIDS patients than in other immunosuppressive disorders has suggested that the presence of human immunodeficiency virus type 1 (HIV-1) in the brain may directly or indirectly contribute to the pathogenesis of this disease. In the present study the authors have examined the expression of the JCV genome in both glial and non-glial cells in the presence of HIV-1 regulatory proteins. They find that the HIV-1-encoded trans-regulatory protein tat increases the basal activity of the JCV late promoter, JCV{sub L}, in glial cells. They conclude that the presence of the HIV-1-encoded tat protein ...

1990-05-01

471

Proteomic analysis of the shistosome tegument and its surface membranes

The elucidation on the metabolic products of the {sup 99}mTc-antibody conjugates may provide insights and approaches that would reduce the undesirable deposition of radioactive species in normal tissue. In this investigation, the radiolabeled species in blood, urine, bile and extracts of liver and kidney obtained at different times after the injection of a model antibody, {sup 99}mTc, into mice were analyzed with various chromatographic methods. Ninety-nine to 100% of the radioactivity in serum was associated with intact Mab 170. The radioactivity in liver homogenate extract was strictly protein-bound to either intact Mab or low molecular weight species (LMW). In kidney extracts, the majority of the radioactivity was protein bound {sup 99}mTc, with less than 8% of the activity being non- protein bound . Multiple {sup 99}mTc -containing protein and non-protein species were found in ...

1996-12-01

472

Scientific Electronic Library Online (English)

Abstract in english The tegument surface of the adult schistosome, bounded by a normal plasma membrane overlain by a secreted membranocalyx, holds the key to understanding how schistosomes evade host immune responses. Recent advances in mass spectrometry (MS), and the sequencing of the Schistosoma mansoni transcriptome/genome, have facilitated schistosome proteomics. We detached the tegument from the worm body and enriched its surface membranes by differential extraction, before subjecting t (more) he preparation to liquid chromatography-based proteomics to identify its constituents. The most exposed proteins on live worms were labelled with impearmeant biotinylation reagents, and we also developed methods to isolate the membranocalyx for analysis. We identified transporters for sugars, amino acids, inorganic ions and water, which confirm the importance of the tegument plasma membrane in nutrient acquisition and solute balance. Enzymes, including phosphohydrolases, ...

2006-10-01

473

Proteins of the kidney microvillar membrane

Photochemical reactivity of the homologous proteins. cap alpha. -lactalbumin and lysozyme

Two methods were used to label pig kidney microvillar membrane proteins from the luminal and cytoplasmic surfaces of closed membrane vesicles. The first method was lactoperoxidase-catalysed radioiodination. Lactoperoxidase and glucose oxidase were positioned inside or outside the vesicles, iodination being initiated by adding glucose and "1"2"5I. After electrophoresis of the proteins, asymmetric labelling patterns on radioautographs were observed. However the major disadvantage of this method was the high degree of intramembrane labelling of the fatty acid chains of membrane lipids. The second method overcame this disadvantage. A new hydophilic photoreagent, 3,5-di("1"2"5I)iodo-4-azidobenzenesulphonate, was transported by a Na"+-dependent system into microvillar vesicles, thus permitting labelling from either side of the membrane when the vesicles were photolysed. The activity of several microvillar peptidases survived the labelling reaction ...

474

HSP70, the earliest-induced gene in the zebrafish retina during optic nerve regeneration: Its role in cell survival

The fluorescent behaviour and the photodynamic effect was studied in native and structurally modified lysozyme and ..cap alpha..-lactalbumin. The Tyr residues in lysozyme and ..cap alpha..-lactalbumin show different sensitivities to the photodynamic effect. The effect is zero in the case of Tyr from native lysozyme. In contrast, the Tyr residues in ..cap alpha..-lactalbumin are susceptible to photooxidation, which indicates a greater degree of exposure to the solvent. The three His residues of ..cap alpha..-lactalbumin have different degrees of exposure and show two different kinetics of photooxidation whereas the His residue of lysozyme is photooxidized with a single kinetic. Two photooxidation kinetics were obtained for the Trp residues of both native proteins, an indication that in both cases there are Trp residues that are differently exposed to the solvent. The wavelengths of maximum fluorescent emission of the Trp residues were different for the two ...

1985-04-01

475

British Library Electronic Table of Contents (United Kingdom)

Fish retinal ganglion cells (RGCs) can survive and regrow their axons after optic nerve injury. Injured RGCs express anti-apoptotic proteins, such as Bcl-2, after nerve injury; however, upstream effectors of this anti-apoptotic protein are not yet fully understood. Heat shock proteins (HSPs) play a crucial role in cell survival against various stress conditions. In this study, we focused on HSP70 expression in the zebrafish retina after optic nerve injury. HSP70 mRNA and protein levels increased rapidly 2.3-fold in RGCs by 1-6 h after injury and returned to control levels by 1-3 days. HSP70 transcription is regulated by heat shock factor 1 (HSF1). HSF1 mRNA and phosphorylated-HSF1 protein rapidly increased by 2.2-fold in RGCs 0.5-6 h after injury. Intraocular injection of HSP inhibitor I s...

2011-01-01

476

Expression of ribosomal genes in pea cotyledons at the initial stages of germination

Effects of intersegmental transfers on target location by proteins

The time of appearance of newly synthesized rRNAs and ribosomal proteins (r-proteins) in the ribosomes of pea cotyledons (Pisum sativum L.) during germination was investigated. The ribosomal fraction was isolated and analyzed according to the method of germination of the embryo in the presence of labeled precursors or after pulse labeling of the embryos at different stages of germination. For the identification of newly synthesized rRNAs in the ribosomes we estimated the relative stability of labeled RNAs to the action of RNase, the sedimentation rate, the ability to be methylated in vivo in the presence of (/sup 14/C)CH/sub 3/-methionine, and the localization in the subunits of dissociated ribosomes. The presence of newly synthesized r-proteins in the ribosomes was judged on the basis of the electrophoretic similarity in SDS-disc electrophoresis of labeled polypeptides of purified ribosome preparations and of genuine ...

1986-01-20

477

Aggregation of silica nanoparticles directed by adsorption of lysozyme.

We study a model for a protein searching for a target, using facilitated diffusion, on a DNA molecule confined in a finite volume. The model includes three distinct pathways for facilitated diffusion: (a) sliding - in which the protein diffuses along the contour of the DNA (b) jumping - where the protein travels between two sites along the DNA by three-dimensional diffusion, and finally (c) intersegmental transfer - which allows the protein to move from one site to another by transiently binding both at the same time. The typical search time is calculated using scaling arguments which are verified numerically. Our results suggest that the inclusion of intersegmental transfer (i) decreases the search time considerably (ii) makes the search time much more robust to variations in the parameters of the model and (iii) that the optimal search time occurs in a regime very different than that found for models ...

2008-01-01

478

Development of detection methods for irradiated foods; development of immunological identification of irradiated foods

The interaction of the globular protein lysozyme with silica nanoparticles of diameter 20 nm was studied in a pH range between the isoelectric points (IEPs) of silica and the protein (pH 3-11). The adsorption affinity and capacity of lysozyme on the silica particles is increasing progressively with pH, and the adsorbed protein induces bridging aggregation of the silica particles. Structural properties of the aggregates were studied as a function of pH at a fixed protein-to-silica concentration ratio which corresponds to a surface concentration of protein well below a complete monolayer in the complete-binding regime at pH > 6. Sedimentation studies indicate the presence of compact aggregates at pH 4-6 and a loose flocculated network at pH 7-9, followed by a sharp decrease of aggregate size near the IEP of lysozyme. The structure of the bridged silica aggregates was studied by ...

2011-07-20

479

The lowland forest tree community in Malinau, Kalimantan (Indonesian Borneo): results from a one-hectare plot

Enzyme-linked Immunosorbent assay systems for the identification of irradiated egg, pork and chicken was developed. Eggs were irradiated in their shells to 0.5{approx}7kGy. Pork was irradiated to 0.5{approx}3kGy and chicken irradiated to 0.5kGy{approx}5kGy. The most sensitive proteins to irradiation were screened by SDS-PAGE and purified. Ovalbumin from egg, salt soluble protein(p) from pork, and salt soluble protein(c) from chicken showed the most sensitivity to irradiation. To investigate for a practical use in identifying of irradiated egg, pork and chicken, competitive ELISA was performed. The binding activity of ovalbumin to anti-ovalbumin IgG was reduced in a dose-dependent manner by irradiating up to 7kGy, and considerably lowered after irradiating at 7kGy. The concentration of 50% inhibition of ovalbumin to IgG was increased to 1.5(0.5kGy){approx}3.7(7kGy) times in an dose-dependent relationship. The binding ...

2002-04-01

480

British Library Electronic Table of Contents (United Kingdom)

Background: While Borneo's forests are globally recognised for their diverse vegetation many regions remain uncharacterised. Aims: We examine the tree community in one hectare of lowland (hill) forest near the Malinau River. Methods: We objectively sited a 1-ha plot in primary forest. All stems over 30 cm girth were measured and identified. Results: Stem basal area was typical for Asian rain forests, but the numbers of stems (759) and species (205) were high. The most abundant species were Gluta wallichii, Cleistanthus bakonensis and Lithocarpus cantleyanus, while those contributing most to basal area were Shorea venulosa, Dipterocarpus lowii and Calophyllum lowii. Dipterocarpaceae was the dominant family amongst the largest stems and contributes a third of stand basal area (11.5 m2). Thir...

2010-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

481

The development of fast breeder reactors

The Path to Metallicity: Synthesis of CNO Elements inStandard BBN

Modern civilisation is based on substantial utilisation of energy. Rapid industrial development and improvement of living standards in India require energy planners to adequately forecast the energy demand and take appropriate measures in advance. However, the development and establishment of new technology is a slow process, sometimes extending over decades. Hence, energy options based on new technologies need to be planned for much in advance making allowance for uncertainties and delays. Fast Breeder Reactor (FBR) technology is an advanced energy option promising abundant and economic supply of power. Research and development work on FBRs has been conducted at the Indira Gandhi Centre of Atomic Research (IGC) since 1971. The international trends in FBR development are highlighted in this discussion and an overview of some of the research activities at IGC is presented. (author). 8 refs., 7 tab s.

482

Studies on Cercariae from Kuwait Bay. X. Description and surface topography of Cercaria kuwaitae X sp. n. (Digenea: Renicolidae).

We perform an analysis of the production of elements with mass number A {ge} 12 in a standard Big Bang Nucleosynthesis scenario. The goal is to provide a more accurate estimate of the very low and yet poorly explored abundance of such elements, relevant for the pristine Population III stars. We examine the synthesis channels for these elements in a critically revised and updated version of the Wagoner-Kawano code, as well as in a further enlarged version including four additional nuclides and a significantly extended nuclear network. Our results show no major discrepancies with the ones obtained using a smaller nuclear network. The robustness of the standard predictions--the early generation of star developed in a metal-free environment--is confirmed.

2007-02-12

483

Source and mobility of minor and trace elements in a volcanic aquifer system: Mt. Vulture (southern Italy)

A new renicolid cercaria, Cercaria kuwaitae X sp. n., from the prosobranch gastropod Cerithium scabridum from Kuwait Bay is described. The new cercaria is nonstyleted gymnocephalous with voluminous Y-shaped excretory vesicle, flame cell formula 2[(3 + 3 + 3) + (3 + 3 + 3)] = 36, and without caudal fins. Surface topography of the new cercaria is studied by scanning electron microscopy. Domed papillae are abundant on the anterior and lateral aspects of the body, ciliated papillae are sparse dorsally. The ventral sucker bears nine domed papillae. Domed papillae form two circles around the oral sucker with three circles of spines in between. This is the first renicolid cercaria to be recorded in a gastropod from the Arabian Gulf region. PMID:9789783

1997-12-01

484

British Library Electronic Table of Contents (United Kingdom)

In this paper we provide a geochemical investigation on 34 groundwater samples in the Mt. Vulture volcanic aquifer representing one of the most important groundwater resources of the southern Italy pumped for drinking and irrigation supply. The present study includes the first data on the abundance and mobility of minor and trace elements and the thermodynamic considerations on water-rock interaction processes in order to evaluate the conditions of alkali basalt weathering by waters enriched in magma-derived CO2. The results highlight the occurrence of two hydrofacies: bicarbonate alkaline-earth and alkaline waters deriving from low-temperature leaching of volcanic rocks of Mt. Vulture, and bicarbonate-sulfate-alkaline waters (high-salinity waters) related to prolonged water circulation in...

2011-01-01

485

Response of native ungulates to drought in semi arid Kenyan rangeland

British Library Electronic Table of Contents (United Kingdom)

Abstract The distribution and abundance of native ungulates were measured on commercially managed, semi arid rangeland in central Kenya over a 3 year period that encompassed severe drought and above average rainfall. Native ungulate biomass density averaged 5282 kg km 2 over the study and was dominated by elephant (Loxodonta africana), impala (Aepyceros melampus) and dik dik (Madoqua kirkii). Biomass density of domestic cattle (Bos taurus) averaged 2280 kg km 2 during the study. Responses of native ungulates to severe drought were variable. Impala densities were similar to or greater than densities for similar habitat in protected areas, and varied from 12 to 16 km 2 during and following the drought to 24 29 km 2 following above average rainfall. Dik dik densities were also greater than de...

2010-01-01

486

Prospects for the direct detection of the cosmic neutrino background

Persistent halogenated compounds in two typical marine aquaculture zones of South China

The existence of a cosmic neutrino background - the analogue of the cosmic microwave background - is a fundamental prediction of standard big bang cosmology. Up to now, the observational evidence for its existence is rather indirect and rests entirely on cosmological observations of, e.g., the light elemental abundances, the anisotropies in the cosmic microwave background, and the large scale distribution of matter. Here, we review more direct, weak interaction based detection techniques for the cosmic neutrino background in the present epoch and in our local neighbourhood. We show that, with current technology, all proposals are still off by some orders of magnitude in sensitivity to lead to a guaranteed detection of the relic neutrinos. The most promising laboratory search, based on neutrino capture on beta decaying nuclei, may be done in future experiments designed to measure the neutrino mass through decay kinematics. (orig.)

2009-01-15

487

British Library Electronic Table of Contents (United Kingdom)

Samples of two seawater farmed fish (crimson snapper (Lutjanus erythopterus) and snubnose pompano (Trachinotus blochii)), water, air, sediment, fish feed, macroalgae and phytoplankton were collected from two estuarine bays (Daya Bay and Hailing Bay) in South China. The concentrations of persistent halogenated compounds (PHCs) including polybrominated diphenyl ethers (PBDEs), organochlorine pesticides and polychlorinated biphenyls (PCBs) varied widely with the different sample matrices under investigation. The compositional patterns in fish, fish feed, macroalgae and phytoplankton, as well as the good correlations between the abundances of p,pprime-DDT and BDE-209 and their metabolites (i.e., p,pprime-DDD and p,pprime-DDE for p,pprime-DDT and BDE-47 for BDE-209) in fish indicated the occurr...

2011-01-01

488

Measurement of Lake Roosevelt biota in relation to reservoir operations. Appendices 1991

Lugworm exclusion experiment: Responses by deposit feeding worms to biogenic habitat transformations

This report consists of appendices A-F containing the biological data which were collected from Lake Roosevelt, Washington. The data are to be used in the design of a computer model that would predict biological responses of reservoir operations as part of the System Operation Review program. Major components of the model included: Quantification of impacts to phytoplankton, zooplanktons, benthic invertebrates, and fish caused by reservoir drawdowns and low water retention times; quantification of number, distribution, and use of fish food organisms in the reservoir by season; determination of seasonal growth of fish species as related to reservoir operations, prey abundance and utilization; and quantification of entrainment levels of zooplankton and fish as related to reservoir operations and water retention times.

1995-11-05

489

British Library Electronic Table of Contents (United Kingdom)

On six 400 m2 plots over 3 years, we excluded the sessile subduction and conveyer-belt feeding polychaete Arenicola marina which generates a pit-and-mound topography at the sediment surface from intertidal sands near the island of Sylt, Germany. This experiment was used to test whether other abundant deposit feeding polychaetes (the discretely motile and surface feeding ragworm Nereis diversicolor and the subsurface-feeding, motile orbiniid polychaete Scoloplos cf. armiger) benefit from competitive release. Ragworms took advantage from the absence of lugworms. Presumably they responded to a more stable and nutritious surface layer at lugworm exclusion plots (relief from inhibitive bioturbation). Contrary to this, S. cf. armiger was negatively affected by the exclusion of A. marina. It may ...

2006-01-01

490

Fungi of the house dust in Riyadh, Saudi Arabia.

Feeding ecology of Xenoturbella bocki (phylum Xenoturbellida) revealed by genetic barcoding

30 species belonging to 18 genera were isolated from floor dust of 30 homes in Riyadh. Out of them 16 species and 10 genera were isolated from dust of air conditioners of the same homes. The most common genera in floor dust were Aspergillus, Penicillium and Cladosporium. Aspergillus repens, A. amstelodami, A. versicolor, A. fumigatus, Penicillium purpurogenum, P. crustosum, Cladosporium cladosporoides and C. herbarum were frequently isolated. The most abundant genera in air conditioner dust were Aspergillus and Penicillium. Aspergillus fumigatus, A. ochraceus, A. terreus, Penicillium oxalicium and P. crustosum were most frequent species. PMID:2330766

1990-01-01

491

British Library Electronic Table of Contents (United Kingdom)

Abstract The benthic marine worm Xenoturbella is frequently contaminated with molluscan DNA, which had earlier caused confusion resulting in a suggested bivalve relationship. In order to find the source of the contaminant, we have used molluscan sequences derived from Xenoturbella and compared them to barcodes obtained from several individuals of the nonmicroscopic molluscs sharing the same environment as Xenoturbella. Using cytochrome oxidase 1, we found the contaminating sequences to be 98% similar to the bivalve Ennucula tenuis. Using the highly variable D1-D2 region of the large ribosomal subunit in Xenoturbella, we found three distinct species of contaminating molluscs, one of which is 99% similar to the bivalve Abra nitida, one of the most abundant bivalves in the Gullmarsfjord where...

2008-01-01

492

Extragalactic Planetary Nebulae: Observational Challenges & Future Prospects

Dense and diffuse gas in dynamically active clouds

The study of extragalactic planetary nebulae (EPN) is a rapidly expanding field. The advent of powerful new instrumentation such as the PN spectrograph has led to an avalanche of new EPN discoveries both within and between galaxies. We now have thousands of EPN detections in a heterogeneous selection of nearby galaxies and their local environments, dwarfing the combined galactic detection efforts of the last century. Key scientific motivations driving this rapid growth in EPN research and discovery have been the use of the PNLF as a standard candle, as dynamical tracers of their host galaxies and dark matter and as probes of Galactic evolution. This is coupled with the basic utility of PN as laboratories of nebula physics and the consequent comparison with theory where population differences, abundance variations and star formation history within and between stellar systems informs both stellar and galactic evolution. Here we pose some of the burning questions, ...

2004-01-01

493

Comparison of stomach contents and condition of two catfish species living at ambient temperatures and in a heated discharge

We investigate the chemical and observational implications of repetitive transient dense core formation in molecular clouds. We allow a transient density fluctuation to form and disperse over a period of 1 Myr, tracing its chemical evolution. We then allow the same gas immediately to undergo further such formation and dispersion cycles. The chemistry of the dense gas in subsequent cycles is similar to that of the first, and a limit cycle is reached quickly (2 - 3 cycles). Enhancement of hydrocarbon abundances during a specific period of evolution is the strongest indicator of previous dynamical history. The molecular content of the diffuse background gas in the molecular cloud is expected to be strongly enhanced by the core formation and dispersion process. Such enhancement may remain for as long as 0.5 Myr. The frequency of repetitive core formation should strongly determine the level of background molecular enhancement. We also convolve the emission from a ...

2006-01-01

494

Collection and characterization of yellow endosperm sorghums from West Africa for biofortification

The lower condition factors for two catfish species in water heated by effluents from APCo's Glen Lyn, Virginia plant as compared to control areas probably is not due to lack of food as evidenced by macroinvertebrate sampling and the fact that only 4.3 percent of the catfish stomachs were empty. Results suggested that young-of-the-year channel catfish were nonselectively eating food organisms present at both control and heated stations. The difference between stomach contents of flatheat catfish from the heated site and fauna available at that site indicated that fish were either moving out of the heated area to feed or were selectively feeding on organisms (particularly mayflies) which were in the substrate or drifted into the heated area. Stomach contents of the two species and distribution and abundance of the macroinvertebrate benthos community are detailed.

495

British Library Electronic Table of Contents (United Kingdom)

Sorghum is a good candidate crop for breeding to increase provitamin A, i.e., biofortification. Yellow endosperm sorghums contain carotenoids, including precursors of vitamin A, and sorghum is a major staple crop in areas of Asia and Africa where vitamin A deficiency is prevalent. Our objective was to collect and characterize yellow endosperm sorghums as a potential new source of genetic diversity to increase provitamin A content. A set of 164 landraces were collected from southern Niger and northern Nigeria. The most important use of these cultivars was as food. The endosperm exhibited a significant variation in yellow intensity. Lutein, zeaxanthin and ?-carotene were the most abundant carotenoids in the ten landraces with the most intense yellow color. Cluster analysis, principal coordin...

2009-01-01

496

Coir pith-a biosorbent for removal of cesium from aqueous solutions

Chitosan-silica hybrid-coated open tubular column for hydrophilic interaction capillary electrochromatography

Coir pith (CP), an abundantly available lignocellulosic agricultural waste from the local coir industry has been found to be an effective biosorbent for removal of metal ions from aqueous solutions. Transition metal hexacyanoferrates, in particular nickel hexacyano ferrate (NiHCF) also referred to as Prussian blue analogue (PBA), is known to be highly selective for removal of cesium from aqueous solutions. A novel method for the synthesis of PBA inside the porous matrix of coir pith and use of this composite material for the sorption of cesium is described in this paper. Equilibrium experiments showed that the sorption capacity of coir pith nearly doubled due to the modification. Owing to its low cost and rapid sorption kinetics, this hybrid material seems to be one of the most promising compounds for the recovery of cesium from liquid nuclear wastes. (author)

2007-01-04

497

British Library Electronic Table of Contents (United Kingdom)

Abstract A novel and convenient protocol for the preparation of an open-tubular column coated with chitosan-silica hybrid using chitosan and silane-coupling agent (-glycidoxy-propyltrimethoxysilane) was developed for CEC, in which, chitosan was covalently bonded to the inner wall of a fused-silica capillary using -glycidoxy-propyltrimethoxysilane as a cross-linking agent. The stationary phase was hydrophilic due to the chitosan-silica hybrid with abundant amine and hydroxyl functional groups. The chromatographic characteristics of the column were evaluated by the separation of some organic acids and inorganic anions. The column showed good selectivity for nucleotides, aromatic acids, and inorganic anions. The mechanism for the separation of these compounds was primarily based on the hydrop...

2011-01-01

498

Bioremediation of petroleum-contaminated soil on Kwajalein Island: Microbiological characterization and biotreatability studies