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Sample records for absent calcemic activity

  1. Distinct Biochemical Activities of Eyes absent During Drosophila Eye Development

    Meng Jin; Graeme Mardon

    2016-01-01

    Eyes absent (Eya) is a highly conserved transcriptional coactivator and protein phosphatase that plays vital roles in multiple developmental processes from Drosophila to humans. Eya proteins contain a PST (Proline-Serine-Threonine)-rich transactivation domain, a threonine phosphatase motif (TPM), and a tyrosine protein phosphatase domain. Using a genomic rescue system, we find that the PST domain is essential for Eya activity and Dac expression, and the TPM is required for full Eya function. ...

  2. Distinct Biochemical Activities of Eyes absent During Drosophila Eye Development.

    Jin, Meng; Mardon, Graeme

    2016-01-01

    Eyes absent (Eya) is a highly conserved transcriptional coactivator and protein phosphatase that plays vital roles in multiple developmental processes from Drosophila to humans. Eya proteins contain a PST (Proline-Serine-Threonine)-rich transactivation domain, a threonine phosphatase motif (TPM), and a tyrosine protein phosphatase domain. Using a genomic rescue system, we find that the PST domain is essential for Eya activity and Dac expression, and the TPM is required for full Eya function. We also find that the threonine phosphatase activity plays only a minor role during Drosophila eye development and the primary function of the PST and TPM domains is transactivation that can be largely substituted by the heterologous activation domain VP16. Along with our previous results that the tyrosine phosphatase activity of Eya is dispensable for normal Eya function in eye formation, we demonstrate that a primary function of Eya during Drosophila eye development is as a transcriptional coactivator. Moreover, the PST/TPM and the threonine phosphatase activity are not required for in vitro interaction between retinal determination factors. Finally, this work is the first report of an Eya-Ey physical interaction. These findings are particularly important because they highlight the need for an in vivo approach that accurately dissects protein function. PMID:26980695

  3. Head Direction Cell Activity Is Absent in Mice without the Horizontal Semicircular Canals.

    Valerio, Stephane; Taube, Jeffrey S

    2016-01-20

    Head direction (HD) cells fire when an animal faces a particular direction in its environment, and they are thought to represent the neural correlate of the animal's perceived spatial orientation. Previous studies have shown that vestibular information is critical for generating the HD signal but have not delineated whether information from all three semicircular canals or just the horizontal canals, which are primarily sensitive to angular head rotation in the horizontal (yaw) plane, are critical for the signal. Here, we monitored cell activity in the anterodorsal thalamus (ADN), an area known to contain HD cells, in epstatic circler (Ecl) mice, which have a bilateral malformation of the horizontal (lateral) semicircular canals. Ecl mice and their littermates that did not express the mutation (controls) were implanted with recording electrodes in the ADN. Results confirm the important role the horizontal canals play in forming the HD signal. Although normal HD cell activity (Raleigh's r > 0.4) was recorded in control mice, no such activity was found in Ecl mice, although some cells had activity that was mildly modulated by HD (0.4 > r > 0.2). Importantly, we also observed activity in Ecl mice that was best characterized as bursty--a pattern of activity similar to an HD signal but without any preferred firing direction. These results suggest that the neural structure for the HD network remains intact in Ecl mice, but the absence of normal horizontal canals results in an inability to control the network properly and brings about an unstable HD signal. Significance statement: Cells in the anterior dorsal thalamic nucleus normally fire in relation to the animal's directional heading with respect to the environment--so-called head direction cells. To understand how these head direction cells generate their activity, we recorded single-unit activity from the anterior dorsal thalamus in transgenic mice that lack functional horizontal semicircular canals. We show that the

  4. CITIZENSHIPS INVISIBLE, ABSENT STATE

    Luis E. Ocampo Banda

    2008-08-01

    Full Text Available The mexican state lies increasingly absent in various sectors of society. The reality mute and today we are facing new ways of conducting politics, building new subjectivities for the market and for the consumer in replacement of citizen. The rise of organized crime and the appearance of new citizenships are elements that question the essence of state, political entity in nature today and every day more limited, absent and turning its back on citizenship, a citizenship invisible, forced and under construction that can not find to whom endorsement their claims.

  5. Absentéismes scolaires

    Becquemin, Michèle; Couronné, Julie; Mardon, Aurélia

    2014-01-01

    Cet article met en perspective les différentes expressions du manquement à l’obligation scolaire à travers le prisme d’un dispositif de rappel à la loi instauré au sein d’une inspection académique. Quel est le milieu social des élèves signalés absentéistes ? Quels arguments les jeunes et leurs parents avancent-ils face aux représentants de l’institution scolaire ? À partir de l’observation de 60 entretiens de rappels à la loi et de l’étude de 250 dossiers d’élèves signalés, les auteurs expose...

  6. The causal effect of vitamin D binding protein (DBP levels on calcemic and cardiometabolic diseases: a Mendelian randomization study.

    Aaron Leong

    2014-10-01

    Full Text Available BACKGROUND: Observational studies have shown that vitamin D binding protein (DBP levels, a key determinant of 25-hydroxy-vitamin D (25OHD levels, and 25OHD levels themselves both associate with risk of disease. If 25OHD levels have a causal influence on disease, and DBP lies in this causal pathway, then DBP levels should likewise be causally associated with disease. We undertook a Mendelian randomization study to determine whether DBP levels have causal effects on common calcemic and cardiometabolic disease. METHODS AND FINDINGS: We measured DBP and 25OHD levels in 2,254 individuals, followed for up to 10 y, in the Canadian Multicentre Osteoporosis Study (CaMos. Using the single nucleotide polymorphism rs2282679 as an instrumental variable, we applied Mendelian randomization methods to determine the causal effect of DBP on calcemic (osteoporosis and hyperparathyroidism and cardiometabolic diseases (hypertension, type 2 diabetes, coronary artery disease, and stroke and related traits, first in CaMos and then in large-scale genome-wide association study consortia. The effect allele was associated with an age- and sex-adjusted decrease in DBP level of 27.4 mg/l (95% CI 24.7, 30.0; n = 2,254. DBP had a strong observational and causal association with 25OHD levels (p = 3.2 × 10(-19. While DBP levels were observationally associated with calcium and body mass index (BMI, these associations were not supported by causal analyses. Despite well-powered sample sizes from consortia, there were no associations of rs2282679 with any other traits and diseases: fasting glucose (0.00 mmol/l [95% CI -0.01, 0.01]; p = 1.00; n = 46,186; fasting insulin (0.01 pmol/l [95% CI -0.00, 0.01,]; p = 0.22; n = 46,186; BMI (0.00 kg/m(2 [95% CI -0.01, 0.01]; p = 0.80; n = 127,587; bone mineral density (0.01 g/cm(2 [95% CI -0.01, 0.03]; p = 0.36; n = 32,961; mean arterial pressure (-0.06 mm Hg [95% CI -0.19, 0.07]; p = 0.36; n = 28,775; ischemic stroke (odds ratio [OR]  = 1

  7. NOVEL NON-CALCEMIC SECOSTEROIDS THAT ARE PRODUCED BY HUMAN EPIDERMAL KERATINOCYTES PROTECT AGAINST SOLAR RADIATION

    Slominski, Andrzej T.; Janjetovic, Zorica; Kim, Tae-Kang; Wasilewski, Piotr; Rosas, Sofia; Hanna, Sherie; Sayre, Robert M.; Dowdy, John C.; Li, Wei; Tuckey, Robert C.

    2015-01-01

    CYP11A1 hydroxylates the side chain of vitamin D3 (D3) in a sequential fashion [D3→20S(OH)D3→20,23(OH)2D3→ 17,20,23(OH)3D3], in an alternative to the classical pathway of activation [D3→25(OH)D3→1,25(OH)2D3]. The products/intermediates of the pathway can be further modified by the action of CYP27B1. The CYP11A1-derived products are biologically active with functions determined by the lineage of the target cells. This pathway can operate in epidermal keratinocytes. To further define the role o...

  8. Novel non-calcemic secosteroids that are produced by human epidermal keratinocytes protect against solar radiation.

    Slominski, Andrzej T; Janjetovic, Zorica; Kim, Tae-Kang; Wasilewski, Piotr; Rosas, Sofia; Hanna, Sherie; Sayre, Robert M; Dowdy, John C; Li, Wei; Tuckey, Robert C

    2015-04-01

    CYP11A1 hydroxylates the side chain of vitamin D3 (D3) in a sequential fashion [D3→20S(OH)D3→20,23(OH)2D3→17,20,23(OH)3D3], in an alternative to the classical pathway of activation [D3→25(OH)D3→1,25(OH)2D3]. The products/intermediates of the pathway can be further modified by the action of CYP27B1. The CYP11A1-derived products are biologically active with functions determined by the lineage of the target cells. This pathway can operate in epidermal keratinocytes. To further define the role of these novel secosteroids we tested them for protective effects against UVB-induced damage in human epidermal keratinocytes, melanocytes and HaCaT keratinocytes, cultured in vitro. The secosteroids attenuated ROS, H2O2 and NO production by UVB-irradiated keratinocytes and melanocytes, with an efficacy similar to 1,25(OH)2D3, while 25(OH)D3 had lower efficacy. These attenuations were also seen to some extent for the 20(OH)D3 precursor, 20S-hydroxy-7-dehydrocholesterol. These effects were accompanied by upregulation of genes encoding enzymes responsible for defense against oxidative stress. Using immunofluorescent staining we observed that the secosteroids reduced the generation cyclobutane pyrimidine dimers in response to UVB and enhanced expression of p53 phosphorylated at Ser-15, but not at Ser-46. Additional evidence for protection against DNA damage in cells exposed to UVB and treated with secosteroids was provided by the Comet assay where DNA fragmentation was markedly reduced by 20(OH)D3 and 20,23(OH)2D3. In conclusion, novel secosteroids that can be produced by the action of CYP11A1 in epidermal keratinocytes have protective effects against UVB radiation. This article is part of a special issue entitled '17th Vitamin D Workshop'. PMID:25617667

  9. Decrease in water activity due to fluid absent partial melting monitored with water content in biotite in the Western Adamello contact aureole (Italy)

    Siron, Guillaume; Baumgartner, Lukas; Bouvier, Anne-Sophie; Vennemann, Torsten

    2016-04-01

    interpret the concentration of OH- to be influenced by water activity within the rocks, and temperature. Our results confirm that oxy-biotite is a non-negligible component, but in the case of prograde metamorphism we do not interpret this as the consequence of a Ti-oxygen exchange only, but also as the consequence of a decrease in water activity due to partial melting. Bauer, K. K., & Vennemann, T. W. (2014). Analytical methods for the measurement of hydrogen isotope composition and water content in clay minerals by TC/EA. Chemical Geology, 363(C), 229-240. Cesare, B., Satish-Kumar, M., Cruciani, G., Pocker, S., & Nodari, L. (2008). Mineral chemistry of Ti-rich biotite from pegmatite and metapelitic granulites of the Kerala Khondalite Belt (southeast India): Petrology and further insight into titanium substitutions. American Mineralogist, 93(2-3), 327-338. Floess, D., & Baumgartner, L. (2013). Formation of garnet clusters during polyphase metamorphism. Terra Nova, 25(2), 144-150. Munoz, J. L. (1984). F-OH and Cl-OH Exchange in Micas with Applications to Hydrothermal Ore Deposits. Reviews in Mineralogy and Geochemistry, 13, 469-493.

  10. Omphalocele Major with Absent Lower Limb

    Kundal, Vijay Kumar; Gajdhar, Mufique; Kundal, Raksha; Ahmed, Reyaz; Agrawal, Leela Dhar

    2013-01-01

    A newborn delivered by Caesarian section presented with an absent anterior abdominal wall and visible bowel loops and liver. The defect was covered by a thin membrane. The patient had associated absent left lower limb and right foot fusion defect. The patient was haemodynamically stable; general condition was average. No genito-urinary abnormality was detected. The anal opening was present normally, and the patient passed meconium immediately after birth. A diagnosis of omphalocele major with...

  11. Omphalocele major with absent lower limb.

    Kundal, Vijay Kumar; Gajdhar, Mufique; Kundal, Raksha; Ahmed, Reyaz; Agrawal, Leela Dhar

    2015-02-01

    A newborn delivered by Caesarian section presented with an absent anterior abdominal wall and visible bowel loops and liver. The defect was covered by a thin membrane. The patient had associated absent left lower limb and right foot fusion defect. The patient was haemodynamically stable; general condition was average. No genito-urinary abnormality was detected. The anal opening was present normally, and the patient passed meconium immediately after birth. A diagnosis of omphalocele major with amelia was made. The patient was initially managed by topical application of povidone-iodine for eschar formation and epithelisation of the sac. The patient was discharged after 1 week with advice for regular follow-up. PMID:25829718

  12. Disneyland Dads, Disneyland Moms? How Nonresident Parents Spend Time with Absent Children.

    Stewart, Susan D.

    1999-01-01

    Examines gender differences in how nonresident parents spend time with their absent children. Results suggest that nonresident mothers and fathers exhibit a similar pattern of participation in activities with their absent children. Most nonresident parents either engage in only leisure activities with their children or have no contact. (Author/MKA)

  13. Absent Pulmonary Valve Syndrome with Left Hemitruncus

    Ashraf M. Aly

    2012-11-01

    Full Text Available Absent pulmonary valve syndrome (APVS is a rare congenital heart defect, usually associated with tetralogy of Fallot (TOF, although other associations have been described. A pregnant woman was referred to fetal echocardiography clinic from the Maternal Fetal Medicine department due to abnormal findings on routine antenatal ultrasound, showing a pulsatile cystic mass above the left atrium and a suspected TOF. A fetal echocardiogram confirmed the presence of TOF/APVS. The pulsatile cystic mass was the aneurysmally dilated main pulmonary artery. The exact origin of the left pulmonary artery (LPA was not clearly established prenatally. A postnatal echocardiogram of the neonate showed an abnormal origin of the LPA from the ascending aorta (hemitruncus. The neonate subsequently underwent surgical repair with a good outcome. We present a novel case of a TOF/APVS associated with an abnormal origin of the LPA from the ascending aorta.

  14. Genetic influences are virtually absent for trust.

    Paul A M Van Lange

    Full Text Available Over the past decades, numerous twin studies have revealed moderate to high heritability estimates for individual differences in a wide range of human traits, including cognitive ability, psychiatric disorders, and personality traits. Even factors that are generally believed to be environmental in nature have been shown to be under genetic control, albeit modest. Is such heritability also present in social traits that are conceptualized as causes and consequences of social interactions or in other ways strongly shaped by behavior of other people? Here we examine a population-based sample of 1,012 twins and relatives. We show that the genetic influence on generalized trust in other people (trust-in-others: h2 = 5%, ns, and beliefs regarding other people's trust in the self (trust-in-self: h2 = 13%, ns, is virtually absent. As test-retest reliability for both scales were found to be moderate or high (r = .76 and r = .53, respectively in an independent sample, we conclude that all variance in trust is likely to be accounted for by non-shared environmental influences. We show that, relative to cognitive abilities, psychiatric disorders, and classic personality variables, genetic influences are smaller for trust, and propose that experiences with or observations of the behavior of other people shape trust more strongly than other traits.

  15. OFF-LABEL USE OF PAMIDRONATE IN HYPER-CALCEMIC NEWBORN AFFECTED BY SUBCUTANEOUS FAT NECROSIS AFTER THERAPEUTIC HYPOTERMIA

    D. Rodriquez

    2012-08-01

    Full Text Available Background: Subcutaneous fat necrosis is a dermatological disorder with characteristic subcutaneous nodules or plaques. It has a benign nature and generally runs a self-limiting course, although can cause significant and dangerous complications, including hypercalcemia. Outbreak of hypercalcemia occurs even several months after hypothermic treatment in asymptomatic or symptomatic way. Irritability, lethargy, hypotonia, emesis, polyuria, polydipsia, constipation and failure to thrive are the most common symptoms of hypercalcemia while seizures, cardiac arrest and acute or chronic renal failure are the most serious manifestations. Chronic hypercalcemia is also associated with nephrocalcinosis and metastatic calcifications of skin, heart, lever and vessels. Low calcium and vitamine D formula preparation milk, diuretics, such as furosemide, and corticosteroids are first-line treatments. In some refractory case pamidronate is used. Pamidronate is a biphosphonate which can modulate bone turnover by leading hydroxyapatite crystals, and it can inhibit osteoclastic bone resorption. Observation: A male full-term infant weighing 2,300 grams was born after an emergency cesarean section due to fetal heart rate decelerations. His initial Apgar scores were 1 at 1 minute, 4 at 5 minutes and 8 at 15 minutes. Due to hypoxicischaemic encephalopathy (stage 1 of Sarnat classification, with abnormal voltage and paroxysmal activities at electroencephalogram,a whole-body therapeutic hypothermia was initiated before sixth hour of life. It was performed to a target rectal temperature of 33,5°C, for about 36 hours. In the third day of life, he developed characteristic subcutaneous fat necrosis skin lesions, such as firm, indurated plaques and red and violaceous nodules, on the upper back area and buttocks. One month after the hypotermic treatment the infant developed a rapid, asymptomatic increase of calcemia. First his milk was changed to a very low calcium and vitamin

  16. Excitatory and inhibitory learning with absent stimuli

    Wheeler, Daniel S.; Sherwood, Andrew; Holland, Peter C.

    2008-01-01

    Three experiments show that two associatively-activated stimulus representations may engage in excitatory or inhibitory learning, depending on their temporal relationship. Experiment 1a suggested that simultaneously-activated stimulus representations show evidence of inhibitory learning in an acquisition test. Experiment 1b showed similar evidence of inhibition in a summation test. Experiment 2 found that activation of two stimulus representations in a serial compound resulted in excitatory l...

  17. Absent right superior caval vein in situs solitus

    Lytzen, Rebekka; Sundberg, Karin; Vejlstrup, Niels

    2015-01-01

    Introduction In up to 0.07% of the general population, the right anterior cardinal vein obliterates and the left remains open, creating an absent right superior caval vein and a persistent left superior caval vein. Absent right superior caval vein is associated with additional congenital heart...... disease in about half the patients. We wished to study the consequences of absent right superior caval vein as an incidental finding on prenatal ultrasonic malformation screening. Material and methods This is a retrospective case series study of all foetuses diagnosed with absent right superior caval vein...... at the national referral hospital, Rigshospitalet, Denmark, from 2009 to 2012. RESULTS: In total, five cases of absent right superior caval vein were reviewed. No significant associated cardiac, extra-cardiac, or genetic anomalies were found. Postnatal echocardiographies confirmed the diagnosis and...

  18. Minimal absent words in four human genome assemblies.

    Garcia, Sara P; Pinho, Armando J

    2011-01-01

    Minimal absent words have been computed in genomes of organisms from all domains of life. Here, we aim to contribute to the catalogue of human genomic variation by investigating the variation in number and content of minimal absent words within a species, using four human genome assemblies. We compare the reference human genome GRCh37 assembly, the HuRef assembly of the genome of Craig Venter, the NA12878 assembly from cell line GM12878, and the YH assembly of the genome of a Han Chinese individual. We find the variation in number and content of minimal absent words between assemblies more significant for large and very large minimal absent words, where the biases of sequencing and assembly methodologies become more pronounced. Moreover, we find generally greater similarity between the human genome assemblies sequenced with capillary-based technologies (GRCh37 and HuRef) than between the human genome assemblies sequenced with massively parallel technologies (NA12878 and YH). Finally, as expected, we find the overall variation in number and content of minimal absent words within a species to be generally smaller than the variation between species. PMID:22220210

  19. Vampire Selfie: A Curious Case of an Absent Reflection

    Grossman, Joshua M.

    2014-01-01

    This article presents a puzzle for the optics section of an introductory course on reflections. A teacher could ask students to explain the phenomenon of the "vampire selfie" or the absent reflection. How could that be? What physics caused this curious phenomenon? The article explains light refraction and its effect on what we see and…

  20. 42 CFR 31.6 - Personnel absent without leave.

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Personnel absent without leave. 31.6 Section 31.6 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES MEDICAL CARE AND EXAMINATIONS MEDICAL CARE FOR CERTAIN PERSONNEL OF THE COAST GUARD, NATIONAL OCEAN SURVEY, PUBLIC...

  1. Nepotism absent in insect societies - or is it?

    Wenseleers, Tom

    2007-08-01

    Inclusive fitness theory, put forward by English biologist William Hamilton in 1964, is considered by many as the most important addition to the theory of natural selection since Darwin. One prediction of the theory is that animals should often show a tendency to nepotistically favour close relatives. Goodisman et al. (2007) test this theory for the first time using molecular methods in a vespine wasp, the eastern yellowjacket, Vespula maculifrons. Somewhat surprisingly, nepotism was found to be absent. This begs the question why nepotism is predicted by theory, yet in a growing list of species is shown to be absent. Is inclusive fitness theory in trouble? As we show, it is not: costs and constraints explain the general absence of queen rearing nepotism, and nepotism in insect societies in fact is well supported in the context of male rearing and manipulation of colony sex ratios. PMID:17651186

  2. PHACE syndrome and congenitally absent thyroid gland at MR imaging.

    Mamlouk, Mark D; Yu, John-Paul J; Asch, Sarah; Mathes, Erin F

    2016-01-01

    PHACE syndrome is a rare neurocutaneous disorder characterized by posterior fossa malformations, hemangiomas, arterial anomalies, cardiac defects, and abnormalities of the eye. Thyroid disorders associated with PHACE syndrome have been described, although there are limited reports of this rare occurrence. We report a case of PHACE syndrome with congenital hypothyroidism in an infant, for which absent thyroid gland was diagnosed at magnetic resonance imaging. PMID:26995578

  3. Absent posterior semicircular canal: HRCT feature of Waardenburg syndrome

    Sandeep M Mahajan; Manish Pithwa; Apeksha Chavan; Deepti Pimple

    2012-01-01

    Absence of posterior semicircular canal is a rare condition, having been reported with only a few syndromes such as Waardenburg syndrome (WS), Alagellie, CHARGE, and Goldenhaar syndromes. We report a 12- year old male with bilateral absent posterior semicircular canals. These phenotypic characteristics of the patient favored the diagnosis of WS. WS is a rare autosomal dominant condition characterized by sensorineural hearing loss, in conjunction with pigmentary abnormalities and defects of th...

  4. Thrombocytopenia with absent radius in a boy and his uncle.

    Schnur, R E; Eunpu, D L; Zackai, E H

    1987-09-01

    We report a boy and his maternal uncle who have Thrombocytopenia-Absent Radius (TAR) syndrome. The mother of the propositus is normal. A maternal aunt has mild radial hypoplasia, possibly representing partial expression of the syndrome. A review of the literature shows several pedigrees in which relatives other than sibs were affected with TAR. Thus, autosomal recessive inheritance may not account for all cases and alternate modes of transmission should be considered. PMID:3314504

  5. Validity of recall absent schoolchildren to free eye health projects

    Regina Noma; Regina de Souza Carvalho; Newton Kara-José

    2012-01-01

    PURPOSE: To analyze the results of recall absent schoolchildren to eye health projects. METHODS: Cross-sectional study. Visual screening was performed in schoolchildren attending 1st to 4th grades at public schools, from 7 to 10 years-old, to select and forward to complete ophthalmic evaluation. The projects were performed during weekends, at a public school, in the same municipality. Free transportation, food and eyeglasses were offered. A second opportunity of examination was offered to the...

  6. Validity of recall absent schoolchildren to free eye health projects

    Regina Noma

    2012-02-01

    Full Text Available PURPOSE: To analyze the results of recall absent schoolchildren to eye health projects. METHODS: Cross-sectional study. Visual screening was performed in schoolchildren attending 1st to 4th grades at public schools, from 7 to 10 years-old, to select and forward to complete ophthalmic evaluation. The projects were performed during weekends, at a public school, in the same municipality. Free transportation, food and eyeglasses were offered. A second opportunity of examination was offered to the students who were absent from the first call, with the same facilities. RESULTS: 51,509 schoolchildren had their vision tested, 14,651 (28.4% were referred for ophthalmic examination. Of these, 8,683 (59.3% attended the first call, 2,228 (37.3% attended the recall and 25.5% of parents did not take their children to ophthalmic examination. The need for eyeglasses for children who attended the examination was 23.8% and 32.0% in the first opportunity and recall, respectively. The recall increased the coverage in 15.2% (59.3% to 74.5%. CONCLUSION: An expressive number of parents (25.5% did not bring their children to be examined, even at a second opportunity of exam. The facilities offered: access, free examination, transportation and glasses. Children who were absent in the first opportunity and appeared at recall had a greater need for eyeglasses. Recall increased the coverage in 15.2% (59.3% to 74.5% and it is not recommended when financial resources are limited.

  7. Absent posterior semicircular canal: HRCT feature of Waardenburg syndrome

    Sandeep M Mahajan

    2012-01-01

    Full Text Available Absence of posterior semicircular canal is a rare condition, having been reported with only a few syndromes such as Waardenburg syndrome (WS, Alagellie, CHARGE, and Goldenhaar syndromes. We report a 12- year old male with bilateral absent posterior semicircular canals. These phenotypic characteristics of the patient favored the diagnosis of WS. WS is a rare autosomal dominant condition characterized by sensorineural hearing loss, in conjunction with pigmentary abnormalities and defects of the neural crest-derived tissues. The case is presented for its rarity and for its high resolution computed tomography (HRCT temporal bone findings so as to reach a precise diagnosis.

  8. Why are sexually selected weapons almost absent in females?

    Anders BERGLUND

    2013-01-01

    In sex role reversed species,predominantly females evolve sexually selected traits,such as ornaments and/or weapons.Female ornaments are common and their function well documented in many species,whether sex role reversed or not.However,sexually selected female weapons seem totally absent except for small wing spurs in three jacana species,present in both males and females.This poor female weaponry is in sharp contrast to the situation in species with conventional sex roles:males commonly have evolved sexually selected weapons as well as ornaments.At the same time,females in many taxa have naturally selected weapons,used in competition over resources or in predator defence.Why are sexually selected weapons then so rare,almost absent,in females? Here I briefly review weaponry in females and the function of these weapons,conclude that the near absence of sexually selected weapons begs an explanation,and suggest that costs of sexually selected weapons may exceed costs of ornaments.Females are more constrained when evolving sexually selected traits compared to males,at least compared to those males that do not provide direct benefits,as trait costs reduce a female's fecundity.I suggest that this constraining trade-off between trait and fecundity restricts females to evolve ornaments but rarely weapons.The same may apply to paternally investing males.Whether sexually selected weapons actually are more costly than sexually selected ornaments remains to be investigated.

  9. Absent without leave; a neuroenergetic theory of mind wandering

    PeterRKilleen

    2013-07-01

    Full Text Available Absent minded people are not under the control of task-relevant stimuli. According to the Neuroenergetics Theory of attention (NeT, this lack of control is often due to fatigue of the relevant processing units in the brain caused by insufficient resupply of the neuron’s preferred fuel, lactate, from nearby astrocytes. A simple drift model of information processing accounts for response-time statistics in a paradigm often used to study inattention, the Sustained Attention to Response Task (SART. It is suggested that errors and slowing in this fast-paced, response-engaging task may have little to due with inattention. Slower-paced and less response-demanding tasks give greater license for inattention—aka absent-mindedness, mind-wandering. The basic NeT is therefore extended with an ancillary model of attentional drift and recapture. This Markov model, called NEMA, assumes probability λ of lapses of attention from one second to the next, and probability α of drifting back to the attentional state. These parameters measure the strength of attraction back to the task (α, or away to competing mental states or action patterns (λ; their proportion determines the probability of the individual being inattentive at any point in time over the long run. Their values are affected by the fatigue of the brain units they traffic between. The deployment of the model is demonstrated with a data set involving paced responding.

  10. Words leaking from objects: thinking with absent photographs

    Ana Luisa Cruz

    2012-01-01

    Full Text Available The possibilities within notions of the object constitute a special area of interest in my research. As I have come to see it, the object is bounded by – and yet comes to alter- views of representation/re-presentation; it contributes towards academic thinking through its capacity for democratizing and bridging itself towards others – yet it has a history of failing in the exchange of everyday gestures with places seemingly remote from the academia. Although accused of resolution and impermeability, I admittedly cannot part with the word ‘object’. And this creates a tension in my work with photography, where I attempt to articulate a personal view of the photograph as something ultimately unfixed. In this view, writing and photography extend continuously and reciprocally into the virtual and the physical from gestures before the photograph and before the word. This text reflects on these tensions, drawing on notions of affect, potentiality and on ethics to discover traces of the other suggested in the physical, but also the imaginary surface of an object. Following Sherry Turkle’s notion of ‘object’ as evocative, in this text ‘the things I think with’ form narratives that reflect the absence of other(s, and the escaping capacity of absent objects in and out of words.

  11. Asymmetrical Sample Training Produces Asymmetrical Retention Functions in Feature-Present/Feature-Absent Matching in Pigeons

    Grant, Douglas S.; Blatz, Craig W.

    2004-01-01

    Pigeons were trained in a matching task in which samples involved presentation of a white line on a green background (feature-present) or on an otherwise dark key (feature-absent). After asymmetrical training in which one group was initially trained with the feature-present sample and another was initially trained with the feature-absent sample,…

  12. 42 CFR 410.175 - Alien absent from the United States.

    2010-10-01

    ... 42 Public Health 2 2010-10-01 2010-10-01 false Alien absent from the United States. 410.175... Alien absent from the United States. (a) Medicare does not pay Part B benefits for services furnished to... during the first full calendar month the alien is back in the United States....

  13. Overlying surficial deposits and absent areas for the Madison Limestone, Black Hills area, South Dakota.

    U.S. Geological Survey, Department of the Interior — This data set describes areas where the Madison Limestone is directly overlain by surficial deposits, as well as those areas where the Madison Limestone is absent...

  14. Mismatch negativity of sad syllables is absent in patients with major depressive disorder.

    Xiaomei Pang

    Full Text Available BACKGROUND: Major depressive disorder (MDD is an important and highly prevalent mental disorder characterized by anhedonia and a lack of interest in everyday activities. Additionally, patients with MDD appear to have deficits in various cognitive abilities. Although a number of studies investigating the central auditory processing of low-level sound features in patients with MDD have demonstrated that this population exhibits impairments in automatic processing, the influence of emotional voice processing has yet to be addressed. To explore the automatic processing of emotional prosodies in patients with MDD, we analyzed the ability to detect automatic changes using event-related potentials (ERPs. METHOD: This study included 18 patients with MDD and 22 age- and sex-matched healthy controls. Subjects were instructed to watch a silent movie but to ignore the afferent acoustic emotional prosodies presented to both ears while continuous electroencephalographic activity was synchronously recorded. Prosodies included meaningless syllables, such as "dada" spoken with happy, angry, sad, or neutral tones. The mean amplitudes of the ERPs elicited by emotional stimuli and the peak latency of the emotional differential waveforms were analyzed. RESULTS: The sad MMN was absent in patients with MDD, whereas the happy and angry MMN components were similar across groups. The abnormal sad emotional MMN component was not significantly correlated with the HRSD-17 and HAMA scores, respectively. CONCLUSION: The data indicate that patients with MDD are impaired in their ability to automatically process sad prosody, whereas their ability to process happy and angry prosodies remains normal. The dysfunctional sad emotion-related MMN in patients with MDD were not correlated with depression symptoms. The blunted MMN of sad prosodies could be considered a trait of MDD.

  15. Absent Does Not Equal Uninvolved: Predictors of Fathering in Teen Mother Families.

    Danziger, Sandra K.; Radin, Norma

    1990-01-01

    Examined effects of several predictors of father involvement in large sample of single-parent, teenage mother families (N=289). The results of telephone interviews indicated that work behavior of absent fathers had a significant direct effect on participation in childrearing and that minority mothers reported higher rates of paternal involvement.…

  16. Contextual Factors Affect Absent Reference Comprehension in 14-Month-Olds

    Ganea, Patricia A.

    2005-01-01

    How do infants come to understand references to absent objects? 14-month-old infants first learned a name for a novel toy, which was then placed out of view. The infants who listened to a story mentioning the nonvisible object, looked, pointed, and searched for it more often than did infants who heard a story using a different name. Their behavior…

  17. Partial melting of two amphibolites: contrasting experimental results under fluid-absent conditions

    Rushmer, Tracy

    1991-03-01

    A large portion of the lower continental crust may be amphibolitic in composition and without a free fluid phase. As a consequence, H2O-undersaturated or fluid-absent melting of amphibolites may be responsible for the formation of some granites and migmatites produced during major orogenic events. In an attempt to determine the systematics of melting under fluid-absent conditions, a series of piston-cylinder experiments was conducted on two natural amphibolites; one, a meta-alkali basalt (ABA) with a total water content of ˜ wt% contained in hornblende, and the other, a meta-island-arc tholeiite (IAT) which has ˜1 1.3 wt% water contained in hornblende, cummingtonite and biotite. The experimentally determined melting ranges of the two amphibolites showed that the solidus temperatures, and sta temperature interval over which amphibole was stable, were controlled by the amphibolites' different bulk compositions and their resulting metamorphic assemblages. The volume % of melt produced by melting of the two amphibolites were compared with estimated amounts, based on Burnham's (1979) water-melt solubility model and the fluid-absent melting model presented by Clemens and Vielzeuf (1987). The observed melt volumes were greater than estimated. As the water content of melt largely detemines the volume % of melt produced, independent measurements of the water-content of the glass formed during partial melting in the ABA were made by thermogravimetric analyses. The water content of the ABA glass is ˜2 wt%, which is less than the assumed “melt-water” content (water content of the melt) used in previous modeling of fluidabsent anatexis in mafic lithologies. As a consequence, more melt can be expected during fluid-absent partial melting of mafic lower crust, as is observed in the experiments. A modification of the Clemens and Vielzeuf (1987) fluid-absent melting model for mafic compositions has been made using the experimental data available on melting in basaltic systems

  18. Absent upper blind Pouch in a case of tracheo-esophageal fistula

    Man Mohan Harjai

    2015-01-01

    Full Text Available A common upper airway and digestive tract is a rare congenital anomaly that is usually fatal and its exact incidence is not known. It is a diagnostic challenge as it requires high index of suspicion. It should be considered in a neonate with respiratory distress in a non-vigorous baby requiring endotracheal intubation, which is difficult even in expert hand. We present a newborn with suspected tracheo-esophageal fistula that was diagnosed intraoperatively to have absent upper blind pouch of the esophagus and on autopsy found to have laryngeal atresia with absent vocal cords and a common aerodigestive tract continuing distally with trachea. The neonate was ventilated with endotracheal tube (ETT placement which in retrospect we came to know that it was in the esophagus. The neonate also had associated multiple congenital anomalies of VACTERL association. The importance of teamwork between neonatologist, pediatric surgeon, anesthesiologist, and radiologist is highlighted for diagnosis and management of such rare cases.

  19. Fatal systemic air embolism in a neonate with absent aortic valve.

    Muneuchi, Jun; Kuraoka, Ayako; Ochiai, Yoshie; Nishibatake, Makoto; Sese, Akira; Joo, Kunitaka

    2011-08-01

    A 32-year-old pregnant woman was referred at 33 weeks' gestation for prenatal ultrasound demonstrating fetal hydrops due to absent aortic valve with free aortic valve insufficiency. Elective caesarian section at 34 week's gestation was performed. Surgical intervention was planned immediately after labor at which time mitral valve closure and atrial septostomy using cardiopulmonary bypass would be performed. However, before insertion of the cannula for cardiopulmonary bypass, a gush of air from the right atrium was noted. The surgical procedure was abandoned because systemic air embolism was suspected. The child died 2 h after birth. Autopsy showed absent aortic valve with closed foramen ovale and left-ventricular hypertrophy. Microscopic findings showed pulmonary and systemic lymphangiectasis, which caused the introduction of air into systemic venous system by way of lymphatic duct just after birth. PMID:21455752

  20. Pulmonary function testing in infants with tetralogy of Fallot and absent pulmonary valve syndrome

    2015-01-01

    Aim: Absent pulmonary valve syndrome (APVS) is found in 3-6% of patients with Tetralogy of Fallot (TOF). Along with findings of TOF, absence of pulmonary valve tissue results in aneurysmal dilatation of the main and branch pulmonary arteries compressing the trachea, main-stem, and intrapulmonary bronchi leading to obstructive airways disease. Our objective was to review pulmonary function tests (PFT) in TOF-APVS patients. Materials and Methods: Eight PFT were performed on five mechanically...

  1. PHACE(S) Syndrome With Absent Intracranial Internal Carotid Artery and Anomalous Circle of Willis.

    Winter, Pieta R; Itinteang, Tinte; Leadbitter, Philip; FitzJohn, Trevor; Tan, Swee T

    2015-06-01

    The authors present a case of PHACE(S) (posterior fossa malformations, hemangioma, arterial anomalies, cardiac defects, eye anomalies, and sternal cleft or supraumbilical raphe) syndrome with a right-sided segmental infantile hemangioma, and describe in detail, the associated absent ipsilateral intracranial internal carotid artery and anomalous Circle of Willis. Propranolol therapy led to accelerated, complete involution. Nadolol may reduce the theoretical risk of treating PHACE(S) patients with β-blockers. PMID:26080245

  2. Postprandial prolactin suppression appears absent in antipsychotic-treated male patients

    Coello, Klara; Broberg, Brian V; Bak, Nikolaj;

    2015-01-01

    subgroups based on the prolactinogenic liability of their antipsychotic treatment indicated 22 to 65% higher postprandial prolactin levels with high and intermediate prolactinogenic antipsychotics. DISCUSSION: A physiological postprandial suppression of serum prolactin appears absent in antipsychotic......-treated males. Marked variability in fasting prolactin levels may reflect individual variations in the diurnal cycle. Uniform acquisition procedures accounting for diurnal variation and food intake may enhance reliability of prolactin levels in antipsychotic-treated male patients....

  3. Surgical treatment of tetralogy of Fallot with absent pulmonary valve syndrome.

    Tanaka, Yuki; Miyamoto, Takashi; Naito, Yuji; Yoshitake, Shuichi

    2016-06-01

    The patient was a 3-month-old girl weighting 3.6 kg, diagnosed with tetralogy of Fallot and absent pulmonary valve syndrome. We surgically repaired the tetralogy of Fallot by patch closure of the ventricular septal defect, right ventricular outflow tract reconstruction using an expanded polytetrafluoroethylene monocusp patch with a bulging sinus, and removal of the bronchial obstruction by anterior translocation of the pulmonary artery using the Lecompte maneuver. PMID:25792545

  4. The bulk and the tail of minimal absent words in genome sequences

    Aurell, Erik; Innocenti, Nicolas; Zhou, Hai-Jun

    2016-04-01

    Minimal absent words (MAW) of a genomic sequence are subsequences that are absent themselves but the subwords of which are all present in the sequence. The characteristic distribution of genomic MAWs as a function of their length has been observed to be qualitatively similar for all living organisms, the bulk being rather short, and only relatively few being long. It has been an open issue whether the reason behind this phenomenon is statistical or reflects a biological mechanism, and what biological information is contained in absent words. In this work we demonstrate that the bulk can be described by a probabilistic model of sampling words from random sequences, while the tail of long MAWs is of biological origin. We introduce the concept of a core of a MAW, which are sequences present in the genome and closest to a given MAW. We show that in E. faecalis, E. coli and yeast the cores of the longest MAWs, which exist in two or more copies, are located in highly conserved regions the most prominent example being ribosomal RNAs. We also show that while the distribution of the cores of long MAWs is roughly uniform over these genomes on a coarse-grained level, on a more detailed level it is strongly enhanced in 3’ untranslated regions (UTRs) and, to a lesser extent, also in 5’ UTRs. This indicates that MAWs and associated MAW cores correspond to fine-tuned evolutionary relationships, and suggest that they can be more widely used as markers for genomic complexity.

  5. Abordagem multifatorial do absenteísmo por doença em trabalhadores de enfermagem

    Roberta Carolina Ferreira

    2012-04-01

    Full Text Available OBJETIVO: Analisar fatores associados ao absenteísmo por doença autorreferido em trabalhadores de enfermagem. MÉTODOS: Estudo transversal com 1.509 trabalhadores de três hospitais públicos no município do Rio de Janeiro, RJ, em 2006. O absenteísmo foi classificado em três níveis: nenhum dia, poucos dias (um a nove dias e muitos dias (> 10 dias, a partir da resposta a uma pergunta do questionário de avaliação do índice de capacidade para o trabalho. As análises de regressão logística levaram em conta um modelo conceitual com base em determinantes distais (condições socioeconômicas, de níveis intermediários I (características ocupacionais e II (características do estilo de vida, e proximais (doenças e condições de saúde. RESULTADOS: As frequências de absenteísmo por doença foram de 20,3% e 16,6% para poucos e muitos dias, respectivamente. Aqueles que referiram mais de um emprego, doenças osteomusculares e avaliaram sua saúde como ruim ou regular apresentaram chances mais elevadas de absenteísmo. Comparados aos enfermeiros, os auxiliares tiveram menor chance de referir poucos dias e os técnicos, maiores chances de apresentar muitos dias de ausência. Chances mais elevadas de referir muitos dias de ausência foram observadas entre os servidores públicos em relação aos contratados (OR = 3,12; IC95% 1,86;5,22 e entre os casados (OR = 1,73; IC95% 1,14;2,63 e separados, divorciados e viúvos (OR = 2,06; IC95% 1,27;3,35, comparados aos solteiros. CONCLUSÕES: Diferentes variáveis foram associadas às duas modalidades de absenteísmo, o que sugere sua determinação múltipla e complexa, relacionada a fatores de diversos níveis que não podem ser explicados apenas por problemas de saúde.

  6. Multi-slice computed tomography assessment of bronchial compression with absent pulmonary valve

    Zhong, Yu-Min; Sun, Ai-Min; Wang, Qian; Zhu, Ming; Qiu, Hai-Sheng [Shanghai Children' s Medical Center and Shanghai Jiao Tong University Medical School, Department of Radiology, Shanghai (China); Jaffe, Richard B. [Primary Children' s Medical Center, Department of Medical Imaging, Salt Lake City, UT (United States); Liu, Jin-Fen [Shanghai Children' s Medical Center, Department of Cardiothoracic Surgery, Shanghai (China); Gao, Wei [Shanghai Children' s Medical Center and Shanghai Jiao Tong University Medical School, Department of Cardiology, Shanghai (China); Berdon, Walter E. [Children' s Hospital of New York, Department of Radiology, New York, NY (United States)

    2014-07-15

    Absent pulmonary valve is a rare cardiovascular anomaly that can result in profound tracheobronchial compression. To demonstrate the advantage of multi-slice CT in diagnosing tracheobronchial compression, its severity as related to the adjacent dilated pulmonary arteries, and associated lung and cardiac lesions. We included children with absent pulmonary valve who were reviewed by multi-slice CT during a 17-year period. The number and locations of stenoses and lung lesions were noted and the severity of stenosis was categorized. The diameter of the pulmonary artery was measured and associated cardiac defects were demonstrated. Thirty-one children (14 girls and 17 boys) were included. Of these, 29 had ventricular septal defect and 2 had an intact ventricular septum. Twenty-nine children (94%) had tracheobronchial compression, judged to be mild in nine children (31%), moderate in 10 (34%) and severe in 10 (34%). The different locations of the stenosis (carina, main bronchi, lobar and segmental bronchi) were observed. And the number and location of lung lesions demonstrated that the right middle and left upper and lower lobes were often affected. The diameter of the pulmonary artery in these children was well above normal published values, and Spearman rank correlation analysis showed a correlation between the size of the pulmonary artery and the severity of the tracheobronchial stenosis. Nineteen children (61%) underwent surgery and 4 of these children had a multi-slice CT post-operative follow-up study. Absent pulmonary valve can cause significant morbidity and mortality in children. Multi-slice CT can accurately depict areas of tracheobronchial compression, associated lung lesions and cardiac defects, helping to direct the surgeon. (orig.)

  7. Multi-slice computed tomography assessment of bronchial compression with absent pulmonary valve

    Absent pulmonary valve is a rare cardiovascular anomaly that can result in profound tracheobronchial compression. To demonstrate the advantage of multi-slice CT in diagnosing tracheobronchial compression, its severity as related to the adjacent dilated pulmonary arteries, and associated lung and cardiac lesions. We included children with absent pulmonary valve who were reviewed by multi-slice CT during a 17-year period. The number and locations of stenoses and lung lesions were noted and the severity of stenosis was categorized. The diameter of the pulmonary artery was measured and associated cardiac defects were demonstrated. Thirty-one children (14 girls and 17 boys) were included. Of these, 29 had ventricular septal defect and 2 had an intact ventricular septum. Twenty-nine children (94%) had tracheobronchial compression, judged to be mild in nine children (31%), moderate in 10 (34%) and severe in 10 (34%). The different locations of the stenosis (carina, main bronchi, lobar and segmental bronchi) were observed. And the number and location of lung lesions demonstrated that the right middle and left upper and lower lobes were often affected. The diameter of the pulmonary artery in these children was well above normal published values, and Spearman rank correlation analysis showed a correlation between the size of the pulmonary artery and the severity of the tracheobronchial stenosis. Nineteen children (61%) underwent surgery and 4 of these children had a multi-slice CT post-operative follow-up study. Absent pulmonary valve can cause significant morbidity and mortality in children. Multi-slice CT can accurately depict areas of tracheobronchial compression, associated lung lesions and cardiac defects, helping to direct the surgeon. (orig.)

  8. Absent right superior caval vein (vena cava) with normal atrial arrangement.

    Choi, J. Y.; Anderson, R H; Macartney, F J

    1987-01-01

    Eight cases of absent right superior caval vein (vena cava) with normal atrial arrangement from the Great Ormond Street database were reviewed. In each case the right subclavian vein and jugular vein drained into a persistent left superior caval vein through a bridging vein. In six cases the left superior caval vein drained into the right atrium by way of the coronary sinus, and in two cases it drained into the left atrium directly because of the complete unroofing of the coronary sinus. The ...

  9. Perinatal outcome of growth restricted fetus with absent end diastolic umbilical blood flow: Case report

    Tasić Marija

    2010-01-01

    Full Text Available Introduction. Absent or reversed end-diastolic blood flow in the umbilical artery is usually associated with poor perinatal outcome and high perinatal mortality rate. Case report. We present the case of a pregnant woman with absent end-diastolic blood flow in the umbilical artery in the 27th week of pregnancy with initial restriction of fetal growth. All though it was more and more obvious that the fetal growth was hindered, the Doppler, cardiotocographic and biophysical parameters did not get any worse as the pregnancy developed. The full fetal maturation was reached after the intense monitoring of the fetal condition and the pregnancy was terminated in the 37lh week by elective Cesarean section. Conclusion. The basic purpose of prenatal fetal monitoring in the situation of hindered fetal growth with chronic hypoxia is to predict the phase of decompensation and to terminate pregnancy before it is developed. The major problem is in great individual variations at the moment of development of decompensation phase, so the major obstetric aim in the monitoring of the fetus hindered in growth is to determine the optimal time and way of delivery.

  10. Computerized axial tomography of the chest for visualization of ''absent'' pulmonary arteries

    To expand the search for central pulmonary arteries in six patients with absence of cardiac-pulmonary continuity, computerized axial tomography (CAT) of the chest was performed. The CAT scans were compared with previous arteriograms and pulmonary vein wedge angiograms. Three patients with type IV truncus arteriosus were studied, and none had a central, right or left pulmonary artery on CAT scan. However, two patients with tetralogy of Fallot with pulmonary atresia and a patent ductus arteriosus to the right lung demonstrated the presence of a left pulmonary artery. In addition, one child with truncus arteriosus with ''absent'' left pulmonary artery demonstrated a left pulmonary artery on the CAT scan. The CAT scan may therefore enhance our ability to search for disconnected pulmonary arteries in children with complex cyanotic congenital heart disease

  11. Overlying surficial deposits and absent areas for the Inyan Kara Formation in the Black Hills area, South Dakota

    U.S. Geological Survey, Department of the Interior — This data set describes areas where the Inyan Kara Group is directly overlain by surficial deposits, as well as those areas where the Inyan Kara Group is absent in...

  12. Genitopatellar syndrome: a new condition comprising absent patellae, scrotal hypoplasia, renal anomalies, facial dysmorphism, and mental retardation

    Cormier-Daire, V.; Chauvet, M.; Lyonnet, S; Briard, M.; Munnich, A; Le Merrer, M

    2000-01-01

    We report on the association of absent patellae, genital and renal anomalies, dysmorphic features, and mental retardation in seven children (six boys and one girl) belonging to five unrelated families. Flexion deformities of the knees and hips with club feet and absent patellae were consistently observed and scrotal hypoplasia and cryptorchidism were present in all boys (6/6). Dysmorphic features included a coarse face, a large nose with a high nasal bridge, and microcephaly. Other features i...

  13. Eukaryotic checkpoints are absent in the cell division cycle of Entamoeba histolytica

    Sulagna Banerjee; Suchismita Das; Anuradha Lohia

    2002-11-01

    Fidelity in transmission of genetic characters is ensured by the faithful duplication of the genome, followed by equal segregation of the genetic material in the progeny. Thus, alternation of DNA duplication (S-phase) and chromosome segregation during the M-phase are hallmarks of most well studied eukaryotes. Several rounds of genome reduplication before chromosome segregation upsets this cycle and leads to polyploidy. Polyploidy is often witnessed in cells prior to differentiation, in embryonic cells or in diseases such as cancer. Studies on the protozoan parasite, Entamoeba histolytica suggest that in its proliferative phase, this organism may accumulate polyploid cells. It has also been shown that although this organism contains sequence homologs of genes which are known to control the cell cycle of most eukaryotes, these genes may be structurally altered and their equivalent function yet to be demonstrated in amoeba. The available information suggests that surveillance mechanisms or ‘checkpoints’ which are known to regulate the eukaryotic cell cycle may be absent or altered in E. histolytica.

  14. Human hepatocytes express absent in melanoma 2 and respond to hepatitis B virus with interleukin-18 expression.

    Pan, Xingfei; Xu, Haixia; Zheng, Changlong; Li, Mei; Zou, Xiaofang; Cao, Hong; Xu, Qihuan

    2016-08-01

    Absent in melanoma 2 (AIM2) is a recently recognized cytoplasmic receptor which could sense cytoplasmic double-stranded DNA (dsDNA). After AIM2 detects the presence of parasitic nucleic acids (dsDNA) derived from invasive bacteria or viral genomes (for example, vaccinia virus and cytomegalovirus) within infected cells, AIM2 inflammasome could be formed. The formed AIM2 inflammasome could induce innate immune response and increase expressions of IL-1β and IL-18. Hepatitis B virus (HBV) is a hepatotropic, non-cytopathic double-stranded DNA virus. The immune response to viral antigens or virus is thought to be responsible for both liver damage and viral clearance in patients with HBV infection. However, there are no reports about whether AIM2 inflammasome exists in hepatocytes. In the present study, we investigated the presence and activity of AIM2 inflammasome in human hepatocytes. We found that AIM2 was expressed in cytoplasm of hepatocytes, and IL-18 expression was increased after AIM2 sensed HBV in hepatocytes in vitro. These results showed that AIM2 inflammasome was active in hepatocytes. We also found that hepatic AIM2 expression of chronic hepatitis B (CHB) patients was higher than that of controls. Hepatic AIM2 expression levels were positively correlated to the severity of liver inflammation. IL-18 is already considered to be associated with hepatic injury during HBV infection. In conclusion, we, therefore, believe that AIM2 inflammasome in hepatocytes might play an important role in the development and maintenance of HBV-related hepatitis. PMID:27094165

  15. The Concept of the Absent Curriculum: The Case of the Muslim Contribution and the English National Curriculum for History

    Wilkinson, Matthew L. N.

    2014-01-01

    This paper introduces the concept of the "absent curriculum" on the premise that the study of curriculum has been prone to privileging curricular presence to the exclusion of curricular absence. In order to address this imbalance and to articulate a theory of absence in the curriculum, the paper applies ideas derived from the philosophy…

  16. Molecular mechanism of male differentiation is conserved in the SRY-absent mammal, Tokudaia osimensis.

    Otake, Tomofumi; Kuroiwa, Asato

    2016-01-01

    The sex-determining gene SRY induces SOX9 expression in the testes of eutherian mammals via two pathways. SRY binds to testis-specific enhancer of Sox9 (TESCO) with SF1 to activate SOX9 transcription. SRY also up-regulates ER71 expression, and ER71 activates Sox9 transcription. After the initiation of testis differentiation, SOX9 enhances Amh expression by binding to its promoter with SF1. SOX8, SOX9 and SOX10, members of the SOXE gene family, also enhance the activities of the Amh promoter and TESCO. In this study, we investigated the regulation of these sexual differentiation genes in Tokudaia osimensis, which lacks a Y chromosome and the SRY gene. The activity of the AMH promoter was stimulated by SOXE genes and SF1. Mutant AMH promoters, with mutations in its SOX and SF1 binding sites, did not show significant activity by SOX9 and SF1. These results indicate that AMH expression was regulated by the binding of SOX9 and SF1. By contrast, SOXE genes could not enhance TESCO activity. These results indicate that TESCO enhancer activity was lost in this species. Furthermore, the activity of the SOX9 promoter was enhanced by ER71, indicating that ER71 may play an important role in the testis-specific expression of SOX9. PMID:27611740

  17. The plight of absent fathers caused by migrant work: Its traumatic impact on adolescent male children in Zimbabwe

    Ananias K. Nyanjaya

    2012-01-01

    Full Text Available This article revealed the degree of trauma experienced by male adolescents when their fathers are absent. The cost of this absence could not be balanced with the material benefits the children have enjoyed, for the benefits have been outweighed by the trauma that children experience in the absence of their fathers. The emotions and tears expressed during the research journey have revealed that material support cannot compensate for the love and presence children expect from their fathers. The deep hurt instilled in their hearts by the periods of absence angered them and led to traumatic experiences. The protracted period of living with only one primary caregiver has imprisoned them into the feminised environment, thereby robbing them of a male identity. Therefore, this article was devoted to creating a shepherding model of caring for boys whose fathers are absent.

  18. Large-scale remapping of visual cortex is absent in adult humans with macular degeneration.

    Baseler, Heidi A; Gouws, André; Haak, Koen V; Racey, Christopher; Crossland, Michael D; Tufail, Adnan; Rubin, Gary S; Cornelissen, Frans W; Morland, Antony B

    2011-05-01

    The occipital lobe contains retinotopic representations of the visual field. The representation of the central retina in early visual areas (V1-3) is found at the occipital pole. When the central retina is lesioned in both eyes by macular degeneration, this region of visual cortex at the occipital pole is accordingly deprived of input. However, even when such lesions occur in adulthood, some visually driven activity in and around the occipital pole can be observed. It has been suggested that this activity is a result of remapping of this area so that it now responds to inputs from intact, peripheral retina. We evaluated whether or not remapping of visual cortex underlies this activity. Our functional magnetic resonance imaging results provide no evidence of remapping, questioning the contemporary view that early visual areas of the adult human brain have the capacity to reorganize extensively. PMID:21441924

  19. Intralimb Coordination Patterns in Absent, Mild, and Severe Stages of Diabetic Neuropathy: Looking Beyond Kinematic Analysis of Gait Cycle

    Liu Chiao Yi; Cristina D Sartor; Francis Trombini Souza; Isabel C. N. Sacco

    2016-01-01

    Aim Diabetes Mellitus progressively leads to impairments in stability and joint motion and might affect coordination patterns, mainly due to neuropathy. This study aims to describe changes in intralimb joint coordination in healthy individuals and patients with absent, mild and, severe stages of neuropathy. Methods Forty-seven diabetic patients were classified into three groups of neuropathic severity by a fuzzy model: 18 without neuropathy (DIAB), 7 with mild neuropathy (MILD), and 22 with m...

  20. Late-onset primary hyperoxaluria type 1 in a Chinese individual with absent alanine:glyoxylate aminotransferase activity

    黃炳南; 唐美華; 麥肇嘉; 盧建宜; 黃煜; 黃矩民

    2004-01-01

    @@ Nephrolithiasis is a common clinical problem, and its cause is often classified as idiopathic. Primary hyperoxaluria, mostly type 1, constitutes one of the rare causes of recurrent nephrolithiasis, but its diagnosis is often missed or delayed. The exact prevalence of primary hyperoxaluria type 1 (PH1), therefore, has been unclear. The reported prevalence varies in different countries. No Chinese PH1 has ever been reported in the literature. We report a rare case of late-onset primary hyperoxaluria, which was diagnosed only after the development of end-stage renal failure. To our knowledge, this case is the first confirmed Chinese PH1.

  1. Degree Of Diminution In Vagal-Cardiac Activity Predicts Sudden Death In Familial Dysautonomia When Resting Tachycardia Is Absent

    Schlegel, T. T.; Marthol, H.; Bucchner, S.; Tutaj, M.; Berlin, D.; Axelrod, F. B.; Hilz, M. J.

    2004-01-01

    Patients with familial dysautonomia (FD) have an increased risk of sudden death, but sensitive and specific predictors of sudden death in FD are lacking. Methods. We recorded 10-min resting high-fidelity 12-lead ECGs in 14 FD patients and in 14 age/gender-matched healthy subjects and studied 25+ different heart rate variability (HRV) indices for their ability to predict sudden death in the FD patients. Indices studied included those from 4 "nonlinear" HRV techniques (detrended fluctuation analysis, approximate entropy, correlation dimension, and PoincarC analyses). The predictive value of PR, QRS, QTc and JTc intervals, QT dispersion (QTd), beat-to-beat QT and PR interval variability indices (QTVI and PRVI) and 12- lead high frequency QRS ECG (150-250 Hz) were also studied. FD patients and controls (C) differed (Pless than 0.0l) with respect to 20+ of the HRV indices (FD less than C) and with respect to QTVI and PRVI (FDBC) and HF QRS- related root mean squared voltages (FDBC) and reduced amplitude zone counts (FD less than C). They differed less with respect to PR intervals (FD less than C) and JTc intervals (FD greater than C) (P less than 0.05 for both) and did not differ at all with respect to QRS and QTc intervals and to QTd. Within 12 months after study, 2 of the 14 patients succumbed to sudden cardiac arrest. The best predictor of sudden death was the degree of diminution in HRV vagal-cardiac (parasympathetic) parameters such as RMSSD, the SDl of Poincare plots, and HF spectral power. Excluding the two FD patients who had resting tachycardia (HR greater than 100, which confounds traditional HRV analyses), the following criteria were independently 100% sensitive and 100% specific for predicting sudden death in the remaining 12 FD patients during spontaneous breathing: RMSSD less than 13 ms and/or PoincarC SD1 less than 9 ms. In FD patients without supine tachycardia, the degree of diminution in parasympathetic HRV parameters (by high-fidelity ECG) predicts incipient death.

  2. Mismatch Negativity of Sad Syllables Is Absent in Patients with Major Depressive Disorder

    Pang, Xiaomei; Xu, Jing; Chang, Yi; Tang, Di; Zheng, Ya; Liu, Yanhua; Sun, Yiming

    2014-01-01

    Background Major depressive disorder (MDD) is an important and highly prevalent mental disorder characterized by anhedonia and a lack of interest in everyday activities. Additionally, patients with MDD appear to have deficits in various cognitive abilities. Although a number of studies investigating the central auditory processing of low-level sound features in patients with MDD have demonstrated that this population exhibits impairments in automatic processing, the influence of emotional voi...

  3. Absent effect of zinc deficiency on the oxidative stress of erythrocytes in chronic uremic rats.

    Chen, Shu-Ming; Wang, Ching-Chu; Lin, Fanny; Young, Tze-Kong

    2002-03-31

    Both anemia and zinc deficiency are commonly observed in patients with chronic uremia. Oxidative stress of red blood cells (RBC) has been suggested to participate in the development of anemia in these patients with chronic uremia due to reduced life span of RBC. Whether zinc deficiency aggravates the effect of oxidative stress on RBC of chronic uremia is still not understood. We thus performed the study to determine the influence of zinc deficiency on the oxidative stress of RBC in uremic rats. Zinc deficiency was induced by long-term dietary zinc deficiency. Five-sixth nephrectomy (5/6 Nx) was used to produce chronic uremia. Experiment was carried out in the following five groups: normal control (NL), chronic uremia (Nx), chronic uremia + dietary zinc deficiency (Nx-D), Nx-D + zinc supplement (Nx-DZ) and Chronic uremia + pair-fed (Nx-PF). Osmotic fragility and lipid peroxidation of RBC were used to evaluate the oxidative stress of RBC. Five weeks after 5/6 nephrectomy (Nx), 5/6 Nx rats present a syndrome of uremia to elevate the levels of plasma creatinine and urea, and reduce the level of plasma zinc (1.12 +/- 0.08 vs 1.35 +/- 0.05 ug/ml). But they does not find to produce anemia and to increase osmotic fragility and lipid peroxidation in RBC. Dietary zinc deficiency in Nx-D group produced severe anorexia and reduced plasma zinc and selenium levels and the activity of RBC-GPX. Yet in Nx-D rats, osmotic fragility and susceptibility of lipid peroxidation in red cells did not increase, because of the increase of plasma copper level (1.85 +/- 0.3 vs 1.41 +/- 0.05 microg/ml) and RBC-SOD activity (1.95 +/- 0.27 vs 0.78 +/- 0.05 unit/g Hb). Zinc supplement in Nx-D rats (Nx-DZ group) recovered the appetite and normalized the levels of plasma zinc, copper and selenium. Food restriction in 5/6 Nx rats (Nx-PF group) decreased plasma copper level and increased osmotic fragility of RBC and elevated the susceptibility of lipid peroxidation after stressing RBC with H2O2 Because

  4. Absenteísmo-doença segundo autorrelato de servidores públicos municipais em Belo Horizonte

    Celeste de Souza Rodrigues

    2013-01-01

    Full Text Available O objetivo do estudo é analisar o perfil de um conjunto de servidores públicos municipais que relataram, por ocasião de um inquérito, absenteísmo-doença nos últimos 12 meses. Em 2009 obteve-se uma amostra de 5.646 (14% do universo de 38.304 servidores municipais de Belo Horizonte. Um questionário foi disponibilizado em sítio da Internet, cuja entrada dependia do consentimento do respondente. Regressão de Poisson foi utilizada para determinar os fatores associados. A prevalência do absenteísmo-doença foi maior entre os servidores do sexo feminino (RP 1,12 e diminuiu com o aumento das faixas etárias: 30-39 anos (RP 0,79; 40-49 anos (RP 0,72; e 50 anos ou mais (RP 0,68. Absenteísmo-doença mostrou-se associado às atividades ligadas aos grupos de fiscalização e vigilância (RP 1,46, serviços gerais (RP 1,22, informação, educação e cultura (RP 1,50 e saúde (RP 1,50. As comorbidades mais presentes foram LER/Dort (RP 1,19, lombalgia (RP 1,16, depressão/ ansiedade (RP 1,20 e percepção de cansaço mental (RP 1,12. O relato de uso de medicação para depressão/ansiedade se mostrou fator protetor (RP 0,82. Maior probabilidade de transtorno mental (RP 1,23 segundo o SRQ20, insatisfação com a capacidade de trabalho (RP 1,08, condições razoáveis (RP 1,21 e condições precárias de trabalho (RP 1,19 foram associadas ao desfecho. Programas de promoção da saúde no trabalho visando a prevenção de comorbidades musculoesqueléticas e queixas psicossociais atenuariam as situações que provavelmente estão na origem do absenteísmo-doença.

  5. Identification and Analysis of Genes Present in Leptospira interrogens serovar lai but Absent in L.biflexa serovar monvalerio

    Ping HE; Xiang-Yan ZHANG; Xiao-Kui GUO; Bao-Yu HU; Xiao-Tian HUANG; Yang YANG; Guo-Ping ZHAO

    2004-01-01

    Genes present in virulent bacterial strains but absent in avirulent close relatives can be of great biologic and clinical interest. This project aimed to identify strain specific DNA sequences of Leptospira interrogens serovar lai, which is absent in the saprophytic L. biflexa serovar monvalerio, via suppression subtractive hybridization with the former as the tester while the latter as the driver. The mixture of PCR amplified DNA fragments from two subtractive hybridization experiments were cloned into pMD 18-T vector and the positive clones were identified by dot blotting against the chromosome DNA of the two strains individually. After DNA sequencing and analysis, the distribution of these genomic fragment sequences in a panel of pathogenic and nonpathogenic leptospires was investigated employing dot blot analysis. Among the 188 positive clones randomly chosen, 24 contained the tester strain specific genomic regions, of which, 5were non-coding fragments while the others contained 23 distinct protein coding sequences. Besides 9 genes encoding functional proteins, 12 genes encode unknown proteins and the rest two genes encode proteins with recognizable domain structures, one for a putative leucine-rich repeats (LRR) family protein while the other as an outer-membrane protein. Our experiment results indicated that suppression subtractive hybridization is effective for screening specific DNA sequences between two leptospiral strains, and some of these sequences might be responsible for virulence determination. Further analysis of these DNA sequences will provide important information on the pathogenesis of Leptospira.

  6. Absent in melanoma 2 (AIM2) expressed in human dental pulp mediates IL-1β secretion in response to cytoplasmic DNA.

    Huang, Shuheng; Song, Zhi; Jiang, Lan; Chen, Lingling; Wang, Runfu; Qin, Wei; Liu, Pengcheng; Lin, Zhengmei

    2015-04-01

    The inflammasome has been determined to play an important role in inflammatory diseases in recent years. Absent in melanoma 2 (AIM2), an inflammasome that recognizes cytoplasmic DNA, has recently been identified as a critical regulator of immune responses. In this study, we explored whether AIM2 was expressed in human dental pulp and defined the role of AIM2 in regulating interleukin (IL)-1β secretion. We demonstrated that AIM2 was only detected in the odontoblast layer of healthy dental pulp, whereas strong expression was observed in inflamed dental pulp. Stimulation with interferon gamma (IFN-γ) and cytoplasmic DNA significantly activated the AIM2 inflammasome and increased IL-1β secretion in human dental pulp cells (HDPCs) in a time- and dose-dependent manner. Moreover, the knockdown of AIM2 downregulated both cleaved-caspase-1 expression and IL-1β release in HDPCs. These results suggest that AIM2 expressed in human dental pulp plays an important role in the immune defense by activating the inflammasome signaling pathway. PMID:24986444

  7. Absent cervical spine pedicle and associated congenital spinal abnormalities - a diagnostic trap in a setting of acute trauma: case report

    Congenital spinal abnormalities can easily be misdiagnosed on plain radiographs. Additional imaging is warranted in doubtful cases, especially in a setting of acute trauma. This patient presented at the emergency unit of our university hospital after a motor vehicle accident and was sent to our radiology department for imaging of the cervical spine. Initial clinical examination and plain radiographs of the cervical spine were performed but not conclusive. Additional CT of the neck helped establish the right diagnosis. CT as a three-dimensional imaging modality with the possibility of multiplanar reconstructions allows for the exact diagnosis and exclusion of acute traumatic lesions of the cervical spine, especially in cases of doubtful plain radiographs and when congenital spinal abnormalities like absent cervical spine pedicle with associated spina bifida may insinuate severe trauma

  8. Computerized axial tomography of the chest for visualization of ''absent'' pulmonary arteries

    Sondheimer, H.M. (Upstate Medical Center, Syracuse, NY); Oliphant, M.; Schneider, B.; Kavey, R.E.W.; Blackman, M.S.; Parker, F.B. Jr.

    1982-05-01

    To expand the search for central pulmonary arteries in six patients with absence of cardiac-pulmonary continuity, computerized axial tomography (CAT) of the chest was performed. The CAT scans were compared with previous arteriograms and pulmonary vein wedge angiograms. Three patients with type IV truncus arteriosus were studied, and none had a central, right or left pulmonary artery on CAT scan. However, two patients with tetralogy of Fallot with pulmonary atresia and a patent ductus arteriosus to the right lung demonstrated the presence of a left pulmonary artery. In addition, one child with truncus arteriosus with ''absent'' left pulmonary artery demonstrated a left pulmonary artery on the CAT scan. The CAT scan may therefore enhance our ability to search for disconnected pulmonary arteries in children with complex cyanotic congenital heart disease.

  9. De la gestion de l'absentéisme à la gestion de la santé dans une entreprise de cosmétiques

    Dumas, Marc

    2005-01-01

    L'absentéisme au travail constitue un phénomène social complexe et est considéré comme un problème crucial par les employeurs.L'audit de l'absentéisme maladie conduit par des chercheurs a été réalisé auprès d'une entreprise industrielle confrontée à une augmentation régulière de l'absentéisme maladie au cours des trois dernières années.L'audit a permis de dégager des causes à l'augmentation de l'absentéisme. Après analyse cette première étape a permis de proposer des remèdes et actions favori...

  10. The Depression Anxiety Stress Scales (DASS): detecting anxiety disorder and depression in employees absent from work because of mental health problems

    Nieuwenhuijsen, K; Boer; Verbeek, J.H.A.M.; Blonk, R.W.B.; van Dijk

    2003-01-01

    Aims: To (1) evaluate the psychometric properties and (2) examine the ability to detect cases with anxiety disorder and depression in a population of employees absent from work because of mental health problems.

  11. Parent-absent begging in the Brown-headed Cowbird (Molothrus ater): the role of short-term need and nestmate size

    Rivers, James W.

    2009-01-01

    Although it is well-established that nestlings of many altricial species beg when parents are away from the nest, we have a poor understanding of parent-absent begging in brood parasites, including the proximate factors that may influence begging frequency and intensity. In this study, I examined how parent-absent begging was influenced by competitive asymmetries between host and Brown-headed Cowbird (Molothrus ater) nestlings under disparate levels of short-term need. Food-deprived cowbird n...

  12. Absent in Melanoma 2 (AIM2) limits pro-inflammatory cytokine transcription in cardiomyocytes by inhibiting STAT1 phosphorylation.

    Furrer, Antonia; Hottiger, Michael O; Valaperti, Alan

    2016-06-01

    Interferon (IFN)-γ is highly upregulated during heart inflammation and enhances the production of pro-inflammatory cytokines. Absent in Melanoma 2 (AIM2) is an IFN-inducible protein implicated as a component of the inflammasome. Here we seek to determine the role of AIM2 during inflammation in cardiac cells. We found that the presence of AIM2, but not of the other inflammasome components Nod-like receptor (NLR) NLRP3 or NLRC4, specifically limited the transcription of the pro-inflammatory cytokines interleukin (IL)-6, IP-10, and tumor necrosis factor (TNF)-α in HL-1 mouse cardiomyocytes stimulated with IFN-γ and lipopolysaccharides (LPS). Similarly, AIM2 reduced pro-inflammatory cytokine transcription in primary mouse neonatal cardiomyocytes (MNC), but not in primary mouse neonatal cardiac fibroblasts (MNF). Interestingly, AIM2-dependent reduction of pro-inflammatory cytokines in cardiomyocytes was independent of Caspase-1. Mechanistically, AIM2 reduced pro-inflammatory cytokine transcription in cardiomyocytes by interacting with and inhibiting the phosphorylation of STAT1. In AIM2-depleted cardiomyocytes, increased STAT1 phosphorylation enhanced the NF-κB pathway by promoting NF-κB p65 phosphorylation and acetylation. These results show for the first time that AIM2 plays an important anti-inflammatory, yet inflammasome-independent function in cardiomyocytes. Our findings will help to further understand how the various heart cell types differently react to inflammatory stimuli. PMID:27148820

  13. Mandibuloacral dysplasia and LMNA A529V mutation in Turkish patients with severe skeletal changes and absent breast development.

    Ozer, Leyla; Unsal, Evrim; Aktuna, Suleyman; Baltaci, Volkan; Celikkol, Pelin; Akyigit, Fatma; Sen, Askin; Ayvaz, Ozge; Balci, Sevim

    2016-07-01

    Mandibuloacral dysplasia (MAD) is an autosomal recessive disorder characterized by acroosteolysis (resorption of terminal phalanges), skin changes (hyperpigmentation), clavicular hypoplasia, craniofascial anomalies, a hook nose and prominent eyes, delayed closures of the cranial sutures, lipodystrophy, alopecia, and skeletal anomalies. MAD patients are classified according to lipodystrophy patterns: type A and type B. The vast majority of MAD cases are caused by LMNA gene mutations. MAD patients with type A lipodystrophy (MADA) have been reported to have LMNA R527H, A529V, or A529T mutations. In this report, we describe two MADA patients with progressive skeletal changes, absent breast development, and cataract in addition to the classical MAD phenotype. Both patients were found to be homozygous for the Ala529Val mutation of the LMNA gene. Our female patient is the oldest MADA patient (59 years old) who has ever been reported with the LMNA mutation and also the LMNA Ala529Val mutation. This study is the second report on MADA patients with a homozygous Ala529Val mutation. PMID:27100822

  14. Intralimb Coordination Patterns in Absent, Mild, and Severe Stages of Diabetic Neuropathy: Looking Beyond Kinematic Analysis of Gait Cycle.

    Liu Chiao Yi

    Full Text Available Diabetes Mellitus progressively leads to impairments in stability and joint motion and might affect coordination patterns, mainly due to neuropathy. This study aims to describe changes in intralimb joint coordination in healthy individuals and patients with absent, mild and, severe stages of neuropathy.Forty-seven diabetic patients were classified into three groups of neuropathic severity by a fuzzy model: 18 without neuropathy (DIAB, 7 with mild neuropathy (MILD, and 22 with moderate to severe neuropathy (SVRE. Thirteen healthy subjects were included as controls (CTRL. Continuous relative phase (CRP was calculated at each instant of the gait cycle for each pair of lower limb joints. Analysis of Variance compared each frame of the CRP time series and its standard deviation among groups (α = 5%.For the ankle-hip CRP, the SVRE group presented increased variability at the propulsion phase and a distinct pattern at the propulsion and initial swing phases compared to the DIAB and CTRL groups. For the ankle-knee CRP, the 3 diabetic groups presented more anti-phase ratios than the CTRL group at the midstance, propulsion, and terminal swing phases, with decreased variability at the early stance phase. For the knee-hip CRP, the MILD group showed more in-phase ratio at the early stance and terminal swing phases and lower variability compared to all other groups. All diabetic groups were more in-phase at early the midstance phase (with lower variability than the control group.The low variability and coordination differences of the MILD group showed that gait coordination might be altered not only when frank evidence of neuropathy is present, but also when neuropathy is still incipient. The ankle-knee CRP at the initial swing phase showed distinct patterns for groups from all degrees of neuropathic severity and CTRLs. The ankle-hip CRP pattern distinguished the SVRE patients from other diabetic groups, particularly in the transitional phase from stance to

  15. Identification of novel direct targets of Drosophila Sine oculis and Eyes absent by integration of genome-wide data sets.

    Jin, Meng; Aibar, Sara; Ge, Zhongqi; Chen, Rui; Aerts, Stein; Mardon, Graeme

    2016-07-01

    Drosophila eye development is a complex process that involves many transcription factors (TFs) and interactions with their cofactors and targets. The TF Sine oculis (So) and its cofactor Eyes absent (Eya) are highly conserved and are both necessary and sufficient for eye development. Despite their many important roles during development, the direct targets of So are still largely unknown. Therefore the So-dependent regulatory network governing eye determination and differentiation is poorly understood. In this study, we intersected gene expression profiles of so or eya mutant eye tissue prepared from three different developmental stages and identified 1731 differentially expressed genes across the Drosophila genome. A combination of co-expression analyses and motif discovery identified a set of twelve putative direct So targets, including three known and nine novel targets. We also used our previous So ChIP-seq data to assess motif predictions for So and identified a canonical So binding motif. Finally, we performed in vivo enhancer reporter assays to test predicted enhancers from six candidate target genes and find that at least one enhancer from each gene is expressed in the developing eye disc and that their expression patterns overlap with that of So. We furthermore confirmed that the expression level of predicted direct So targets, for which antibodies are available, are reduced in so or eya post-mitotic knockout eye discs. In summary, we expand the set of putative So targets and show for the first time that the combined use of expression profiling of so with its cofactor eya is an effective method to identify novel So targets. Moreover, since So is highly conserved throughout the metazoa, our results provide the basis for future functional studies in a wide variety of organisms. PMID:27178668

  16. Rod-like microglia are restricted to eyes with laser-induced ocular hypertension but absent from the microglial changes in the contralateral untreated eye.

    Rosa de Hoz

    Full Text Available In the mouse model of unilateral laser-induced ocular hypertension (OHT the microglia in both the treated and the normotensive untreated contralateral eye have morphological signs of activation and up-regulation of MHC-II expression in comparison with naïve. In the brain, rod-like microglia align to less-injured neurons in an effort to limit damage. We investigate whether: i microglial activation is secondary to laser injury or to a higher IOP and; ii the presence of rod-like microglia is related to OHT. Three groups of mice were used: age-matched control (naïve, n=15; and two lasered: limbal (OHT, n=15; and non-draining portion of the sclera (scleral, n=3. In the lasered animals, treated eyes as well as contralateral eyes were analysed. Retinal whole-mounts were immunostained with antibodies against, Iba-1, NF-200, MHC-II, CD86, CD68 and Ym1. In the scleral group (normal ocular pressure no microglial signs of activation were found. Similarly to naïve eyes, OHT-eyes and their contralateral eyes had ramified microglia in the nerve-fibre layer related to the blood vessel. However, only eyes with OHT had rod-like microglia that aligned end-to-end, coupling to form trains of multiple cells running parallel to axons in the retinal surface. Rod-like microglia were CD68+ and were related to retinal ganglion cells (RGCs showing signs of degeneration (NF-200+RGCs. Although MHC-II expression was up-regulated in the microglia of the NFL both in OHT-eyes and their contralateral eyes, no expression of CD86 and Ym1 was detected in ramified or in rod-like microglia. After 15 days of unilateral lasering of the limbal and the non-draining portion of the sclera, activated microglia was restricted to OHT-eyes and their contralateral eyes. However, rod-like microglia were restricted to eyes with OHT and degenerated NF-200+RGCs and were absent from their contralateral eyes. Thus, rod-like microglia seem be related to the neurodegeneration associated with HTO.

  17. Finding the Two-Way Street: Women from Mother-Present/Father-Absent Homes and Their Ability to Make Close Female Friendships

    Marote, Melissa A.

    2011-01-01

    This heuristic study involving seven coresearchers, which included the author, explores the experiences of women from mother-present/father-absent homes and their ability to form and maintain close female friendships. The heuristic research model was chosen to provide the opportunity to conduct research in a very personalized, collaborative way…

  18. Drayer's syndrome of mental retardation, microcephaly, short stature and absent phalanges is caused by a recurrent deletion of chromosome 15(q26.2 -> qter)

    Rump, P.; Dijkhuizen, T.; Sikkema-Raddatz, B.; Lemmink, H. H.; Vos, Y. J.; Verheij, J. B. G. M.; van Ravenswaaij, C. M. A.

    2008-01-01

    We reevaluated a unique family with two sibs who had a presumed autosomal recessively inherited syndrome characterized by mental retardation, microcephaly, short stature and absent phalanges. This family was originally described by Drayer et al. in 1977. Using modern molecular techniques, we demonst

  19. The reproductive ecology of Medicago citrina (Font Quer) Greuter (Leguminosae): a bee-pollinated plant in Mediterranean islands where bees are absent

    Pérez Bañón, Celeste; Juan Gallardo, Ana Isabel; Petanidou, Theodora; Marcos García, María Ángeles; Crespo Villalba, Manuel Benito

    2003-01-01

    The reproductive ecology of Medicago citrina was studied in the Columbretes archipelago (Mediterranean Sea), where bees are absent. The flowers were self-compatible, homogamous, nectar producers, depended on large floral visitors for tripping and pollination. Several characteristics of the reproductive biology of M. citrina indicate an adaptation to an isolated environment characterised by scarcity of appropriate pollinators. As a result of this scarcity, fruit and seed set under natural cond...

  20. Absent otoacoustic emissions predict otitis media in young Aboriginal children: A birth cohort study in Aboriginal and non-Aboriginal children in an arid zone of Western Australia

    Stokes Annette; Finucane Janine; Elsbury Dimity; Jacoby Peter; Weeks Sharon; Lehmann Deborah; Monck Ruth; Coates Harvey

    2008-01-01

    Abstract Background Otitis media (OM) is the most common paediatric illness for which antibiotics are prescribed. In Australian Aboriginal children OM is frequently asymptomatic and starts at a younger age, is more common and more likely to result in hearing loss than in non-Aboriginal children. Absent transient evoked otoacoustic emissions (TEOAEs) may predict subsequent risk of OM. Methods 100 Aboriginal and 180 non-Aboriginal children in a semi-arid zone of Western Australia were followed ...

  1. Taxa de suspensão de cirurgia em um hospital universitário e os motivos de absenteísmo do paciente à cirurgia programada

    Paschoal Maria Lúcia Habib

    2006-01-01

    Full Text Available Os objetivos do estudo foram identificar a taxa de suspensão de cirurgia e verificar os motivos do absenteísmo do paciente à cirurgia programada. O trabalho foi realizado em um hospital universitário, voltado à assistência secundária, no município de São Paulo, no período de três meses e realizada entrevista com 60 pacientes que deixaram de comparecer à cirurgia. Os resultados mostraram taxa de suspensão de cirurgia de 19,91% e o absenteísmo de 54,30% das 186 suspensões de cirurgias. Foram encontrados 10 motivos para o absenteísmo do paciente que, agrupados, puderam ser atribuídos à condição institucional (53,33%, na maioria pela desinformação sobre a data do agendamento cirúrgico; condição clínica (28,33% decorrente de infecções de vias aéreas e outras doenças; condição social (10% pela falta de dinheiro e problemas familiares e condição pessoal (8,33% em conseqüência da desistência do paciente.

  2. Nain-e Havandi Andrographis paniculata present yesterday, absent today: a plenary review on underutilized herb of Iran's pharmaceutical plants.

    Valdiani, Alireza; Kadir, Mihdzar Abdul; Tan, Soon Guan; Talei, Daryush; Abdullah, Mohd Puad; Nikzad, Sonia

    2012-05-01

    Nain-e Havandi (Andrographis paniculata Nees.) (AP) is an annual herbaceous plant belonging to the family Acanthacea. Only a few species of Andrographis genus out of 28 are medicinally concerned of which AP is the most important. Knowledge about the arrival of AP to Iran is extremely lacking but most probably it has been imported from India. However, evidence implies the familiarity of Iran's folkloric medicine with this plant, but it has been disappeared from contemporary medicine for unknown reasons. Presence of active ingredients from diterpenoids group such as andrographolide, neoandrographolide and 14-deoxy-11,12-didehydroandrographolide has given incredible unique medicinal properties to the plant. Traditionally, Nain-e Havandi has been used in the role of a non-farm plant as a remedy for skin problems, flu, respiratory disease, and snakebite in East and Southeast Asia for centuries. Recently, it has been utilized as a treatment for HIV, hepatitis, diabetes, cancer and kidney disorders. Intensive cultivation of the herb started only in the past decade in countries such as China, India, Thailand, Indonesia, West Indies, Mauritius and to some extent, in Malaysia. Availability of different ecological zones in Iran complies with reestablishment of AP in tropical and temperate regions of the country. This is killing two birds with one stone, supporting the conservational and economic aspects. PMID:22198549

  3. Prevalence of dysmenorrhea in university students and its relation to school absenteism, physical exercise and use of medicines

    Janaina Mayer de Oliveira Nunes

    2014-05-01

    Full Text Available Objective: To determine the prevalence of dysmenorrhea in female university students and the frequency of school absenteeism, physical exercise and use of medicines to treat the syndrome. Methods: This is a cross-sectional study conducted with university students using a self-administered questionnaire containing sociodemographic, obstetrical, and gynecological data. The menstrual pain was assessed by the visual analogue scale (VAS. Frequency, percentage, mean, and standard deviation were used for descriptive data analysis. Results: The sample consisted of 130 women aged between 17 and 33 years (20.6 ± 2.7 years. One hundred and twenty four volunteers (95.4% complained of dysmenorrhea. Regarding the intensity, most of the volunteers felt moderate or severe menstrual cramps (51.6% and 36.3%, respectively. Sixty (48.4% participants reported school absenteeism due to menstrual pain and none of them experienced mild pain. Among the volunteers who had moderate or severe dysmenorrhea, only 24 (20.2% practiced physical exercise and most of them (79% needed medicines to treat this syndrome. Conclusion: There was a high prevalence of dysmenorrhea among the university students, and in most cases, the pains limit activities and accounts for school absenteeism. Most women with moderate to severe dysmenorrhea do not practice physical exercise and need to use medicines to treat this syndrome. doi:10.5020/18061230.2013.p381

  4. Absent/weak CD44 intensity and positive human papillomavirus (HPV) status in oropharyngeal squamous cell carcinoma indicates a very high survival

    Patients with human papillomavirus DNA positive (HPVDNA+) oropharyngeal squamous cell carcinoma (OSCC) have better clinical outcome than those with HPV DNA negative (HPVDNA−) OSCC upon intensive oncological treatment. All HPVDNA+ OSCC patients may not require intensive treatment, however, but before potentially deintensifying treatment, additional predictive markers are needed. Here, we examined HPV, p16INK4a, and CD44 in OSCC in correlation to clinical outcome. Pretreatment tumors from 290 OSCC patients, the majority not receiving chemotherapy, were analyzed for HPV DNA by Luminex and for p16INK4a and CD44 by immunohistochemistry. 225/290 (78%) tumors were HPVDNA+ and 211/290 (73%) overexpressed p16INK4a, which correlated to presence of HPV (P < 0.0001). Presence of HPV DNA, absent/weak CD44 intensity staining correlated to favorable 3-year disease-free survival (DFS) and overall survival (OS) by univariate and multivariate analysis, and likewise for p16INK4a by univariate analysis. Upon stratification for HPV, HPVDNA+ OSCC with absent/weak CD44 intensity presented the significantly best 3-year DFS and OS, with >95% 3-year DFS and OS. Furthermore, in HPVDNA+ OSCC, p16INK4a+ overexpression correlated to a favorable 3-year OS. In conclusion, patients with HPVDNA+ and absent/weak CD44 intensity OSCC presented the best survival and this marker combination could possibly be used for selecting patients for tailored deintensified treatment in prospective clinical trials. Absence of/weak CD44 or presence of human papillomavirus (HPV) DNA was shown as a favorable prognostic factors in tonsillar and tongue base cancer. Moreover, patients with the combination of absence of/weak CD44 and presence of HPV DNA presented a very favorable outcome. Therefore, we suggest that this marker combination could potentially be used to single out patients with a high survival that could benefit from a de-escalated oncological treatment

  5. Prevalência de absenteísmo entre trabalhadores do serviço público =Prevalence of absenteeism among public service workers

    Andrade, Tania Bof de et al.

    2008-01-01

    Objetivos: determinar a prevalência e as principais causas de absenteísmo entre os servidores públicos municipais de Vitória, Espírito Santo. Métodos: foi realizado um estudo transversal com uma amostra composta por 400 indivíduos os quais foram aleatoriamente selecionados entre os funcionários cadastrados no Departamento de Medicina do Trabalho da Prefeitura Municipal. A coleta de dados foi feita por meio de fichas padronizadas a partir das informações contidas nos prontuários dos partici...

  6. The α-proteobacteria Wolbachia pipientis protein disulfide machinery has a regulatory mechanism absent in γ-proteobacteria.

    Walden, Patricia M; Halili, Maria A; Archbold, Julia K; Lindahl, Fredrik; Fairlie, David P; Inaba, Kenji; Martin, Jennifer L

    2013-01-01

    The α-proteobacterium Wolbachia pipientis infects more than 65% of insect species worldwide and manipulates the host reproductive machinery to enable its own survival. It can live in mutualistic relationships with hosts that cause human disease, including mosquitoes that carry the Dengue virus. Like many other bacteria, Wolbachia contains disulfide bond forming (Dsb) proteins that introduce disulfide bonds into secreted effector proteins. The genome of the Wolbachia strain wMel encodes two DsbA-like proteins sharing just 21% sequence identity to each other, α-DsbA1 and α-DsbA2, and an integral membrane protein, α-DsbB. α-DsbA1 and α-DsbA2 both have a Cys-X-X-Cys active site that, by analogy with Escherichia coli DsbA, would need to be oxidized to the disulfide form to serve as a disulfide bond donor toward substrate proteins. Here we show that the integral membrane protein α-DsbB oxidizes α-DsbA1, but not α-DsbA2. The interaction between α-DsbA1 and α-DsbB is very specific, involving four essential cysteines located in the two periplasmic loops of α-DsbB. In the electron flow cascade, oxidation of α-DsbA1 by α-DsbB is initiated by an oxidizing quinone cofactor that interacts with the cysteine pair in the first periplasmic loop. Oxidizing power is transferred to the second cysteine pair, which directly interacts with α-DsbA1. This reaction is inhibited by a non-catalytic disulfide present in α-DsbA1, conserved in other α-proteobacterial DsbAs but not in γ-proteobacterial DsbAs. This is the first characterization of the integral membrane protein α-DsbB from Wolbachia and reveals that the non-catalytic cysteines of α-DsbA1 regulate the redox relay system in cooperation with α-DsbB. PMID:24282596

  7. The α-proteobacteria Wolbachia pipientis protein disulfide machinery has a regulatory mechanism absent in γ-proteobacteria.

    Patricia M Walden

    Full Text Available The α-proteobacterium Wolbachia pipientis infects more than 65% of insect species worldwide and manipulates the host reproductive machinery to enable its own survival. It can live in mutualistic relationships with hosts that cause human disease, including mosquitoes that carry the Dengue virus. Like many other bacteria, Wolbachia contains disulfide bond forming (Dsb proteins that introduce disulfide bonds into secreted effector proteins. The genome of the Wolbachia strain wMel encodes two DsbA-like proteins sharing just 21% sequence identity to each other, α-DsbA1 and α-DsbA2, and an integral membrane protein, α-DsbB. α-DsbA1 and α-DsbA2 both have a Cys-X-X-Cys active site that, by analogy with Escherichia coli DsbA, would need to be oxidized to the disulfide form to serve as a disulfide bond donor toward substrate proteins. Here we show that the integral membrane protein α-DsbB oxidizes α-DsbA1, but not α-DsbA2. The interaction between α-DsbA1 and α-DsbB is very specific, involving four essential cysteines located in the two periplasmic loops of α-DsbB. In the electron flow cascade, oxidation of α-DsbA1 by α-DsbB is initiated by an oxidizing quinone cofactor that interacts with the cysteine pair in the first periplasmic loop. Oxidizing power is transferred to the second cysteine pair, which directly interacts with α-DsbA1. This reaction is inhibited by a non-catalytic disulfide present in α-DsbA1, conserved in other α-proteobacterial DsbAs but not in γ-proteobacterial DsbAs. This is the first characterization of the integral membrane protein α-DsbB from Wolbachia and reveals that the non-catalytic cysteines of α-DsbA1 regulate the redox relay system in cooperation with α-DsbB.

  8. Crystallization and preliminary X-ray crystallographic investigations on a βγ-crystallin domain of absent in melanoma 1 (AIM1), a protein from Homo sapiens

    Aravind, Penmatsa; Rajini, Bheemreddy; Sharma, Yogendra; Sankaranarayanan, Rajan, E-mail: sankar@ccmb.res.in [Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007 (India)

    2006-03-01

    The crystallization and preliminary X-ray diffraction analysis of AIM1g1, a βγ-crystallin domain of absent in melanoma (AIM1) protein from H. sapiens, is reported. AIM1g1 is a single βγ-crystallin domain from the protein absent in melanoma 1 (AIM1), which appears to play a role in the suppression of melanomas. This domain is known to bind calcium and its structure would help in identifying calcium-coordinating sites in vertebrate crystallins, which have hitherto been believed to have lost this ability during evolution. Crystallization of this domain was performed by the hanging-drop vapour-diffusion method. Crystals diffracted to a maximum resolution of 1.86 Å and were found to belong to space group P6{sub 1} or P6{sub 5}, with unit-cell parameters a = b = 54.98, c = 59.73 Å. Solvent-content analysis indicated the presence of one monomer per asymmetric unit.

  9. An Amplitude-normalized Pseudo Well-log Construction Method and its Application on AVO Inversion in a Well-absent Marine Area

    Fan Chunyan

    2015-06-01

    Full Text Available AVO inversion is hard to be efficiently applied in unexploited fields due to the insufficiency of well information. For the sake of AVO inversion in a well-absent area, the most conventional method is to construct pseudo well-logs by defining seismic processing velocity as the P-velocity and computing S-velocity and density using empirical formulas, yet the resolution of the corresponding earth models and final inverted results could be extremely low, and a rough formula could destroy the inversion thoroughly. To overcome this problem, an amplitudenormalized pseudo well-log construction method that reconstructs pseudo well-logs in accordance with computed P-wave reflection amplitudes and nearby drilling data is proposed in this paper. It enhances the inversion resolution efficiently with respect to the real elastic parameter relationships, so that the corresponding AVO inversion results are reasonably improved. In summary, the proposed method is successfully applied in the AVO inversion of a well-absent marine area, and could be valuable in the early phase, particularly of the offshore hydrocarbon exploration.

  10. Crystallization and preliminary X-ray crystallographic investigations on a βγ-crystallin domain of absent in melanoma 1 (AIM1), a protein from Homo sapiens

    The crystallization and preliminary X-ray diffraction analysis of AIM1g1, a βγ-crystallin domain of absent in melanoma (AIM1) protein from H. sapiens, is reported. AIM1g1 is a single βγ-crystallin domain from the protein absent in melanoma 1 (AIM1), which appears to play a role in the suppression of melanomas. This domain is known to bind calcium and its structure would help in identifying calcium-coordinating sites in vertebrate crystallins, which have hitherto been believed to have lost this ability during evolution. Crystallization of this domain was performed by the hanging-drop vapour-diffusion method. Crystals diffracted to a maximum resolution of 1.86 Å and were found to belong to space group P61 or P65, with unit-cell parameters a = b = 54.98, c = 59.73 Å. Solvent-content analysis indicated the presence of one monomer per asymmetric unit

  11. Tooth formation - delayed or absent

    ... missing teeth they never developed. Cosmetic or orthodontic dentistry can correct this problem. ... Jenson HB, Stanton BF, eds. Nelson Textbook of Pediatrics. 19th ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap ...

  12. Tooth formation - delayed or absent

    ... are missing teeth they never developed. Cosmetic or orthodontic dentistry can correct this problem. Causes Specific diseases ... herein should not be used during any medical emergency or for the diagnosis or treatment of any ...

  13. The CT appearances of delayed amniotic fluid clearance from the lungs in an infant with absent pulmonary valve and congenital lobar emphysema

    Congenital lobar emphysema (CLE) is a cause of severe neonatal respiratory distress. Overexpansion of the affected pulmonary lobe in the fetus is due to narrowing of the airway, with a resultant 'ball-valve' effect. At birth, there may be delayed clearance of fetal lung fluid. Early chest radiographs show opacification of the hyperexpanded lobe. The CT findings in the immediate neonatal period have not been previously reported. We describe the imaging in a neonate with tetralogy of Fallot and absent pulmonary valve with secondary CLE. CT demonstrates the hyperexpanded lobe with initial thickening of the interlobular septa and alveolar ground glass attenuation, with subsequent clearing. This resorption of fetal lung fluid via the pulmonary interstitium should not be confused with interstitial lung disease. (orig.)

  14. The CT appearances of delayed amniotic fluid clearance from the lungs in an infant with absent pulmonary valve and congenital lobar emphysema

    Fink, A. Michelle [Royal Children' s Hospital, Department of Medical Imaging, Parkville, Victoria (Australia); University of Melbourne, Melbourne, Victoria (Australia); Edis, Brian [Royal Children' s Hospital, Department of Cardiology, Parkville, Victoria (Australia); Massie, John [University of Melbourne, Melbourne, Victoria (Australia); Royal Children' s Hospital, Department of Respiratory Medicine, Parkville, Victoria (Australia); Murdoch Children' s Research Institute, Melbourne, Victoria (Australia)

    2005-09-01

    Congenital lobar emphysema (CLE) is a cause of severe neonatal respiratory distress. Overexpansion of the affected pulmonary lobe in the fetus is due to narrowing of the airway, with a resultant 'ball-valve' effect. At birth, there may be delayed clearance of fetal lung fluid. Early chest radiographs show opacification of the hyperexpanded lobe. The CT findings in the immediate neonatal period have not been previously reported. We describe the imaging in a neonate with tetralogy of Fallot and absent pulmonary valve with secondary CLE. CT demonstrates the hyperexpanded lobe with initial thickening of the interlobular septa and alveolar ground glass attenuation, with subsequent clearing. This resorption of fetal lung fluid via the pulmonary interstitium should not be confused with interstitial lung disease. (orig.)

  15. Positive Family History, Infection, Low Absolute Lymphocyte Count (ALC) and Absent Thymic Shadow: Diagnostic Clues for all Molecular Forms of Severe Combined Immunodeficiency (SCID)

    McWilliams, Laurie M; Railey, Mary Dell; Buckley, Rebecca H

    2015-01-01

    Background Severe Combined Immunodeficiency (SCID) is a syndrome uniformly fatal during infancy unless recognized and treated successfully by bone marrow transplantation or gene therapy. Because SCID infants have no abnormal physical appearance, diagnosis is usually delayed unless newborn screening is performed. Objective In this study, we sought to evaluate the presenting features of all 172 SCID patients transplanted at this institution over the past 31 years. Methods We reviewed original charts from 172 consecutive classic SCID patients who received either T cell-depleted HLA-haploidentical (N=154) or HLA-identical (N=18) non-ablative related marrow transplants at Duke University Medical Center from 1982–2013. Results The mean age at presentation was 4.87 months. When there was a family history of early infant death or known SCID (63/172 or 37%), the mean presentation age was much earlier, 2.0 months compared to 6.6 months. Failure to thrive was common, with 84 patients (50%) having a weight less than the 5th percentile. The leading infections included oral moniliasis (43%), viral infections (61/172 35.5%) and Pneumocystis jiroveci (26%) pneumonia. The group mean ALC was 1454/cmm; 88% of the infants had an ALC less than 3000/cmm. Absent thymic shadow was seen in 92% of infants with electronic radiographic data available. An absence of T cell function was found in all patients. Conclusions SCID infants appear normal at birth but later present with failure to thrive and/or recurrent fungal, viral and bacterial infections. Low ALCs and absent thymic shadow on chest x-ray are key diagnostic clues. The absence of T cell function confirms the diagnosis. PMID:25824440

  16. Fatores de risco para absenteísmo com licença médica em trabalhadores da indústria de petróleo

    Nágila Soares Xavier Oenning

    2014-02-01

    Full Text Available OBJETIVO : Identificar fatores de risco para o absenteísmo com licença médica em trabalhadores de empresa de petróleo. MÉTODOS : Estudo caso-controle (120 casos e 656 controles aninhado a um estudo de coorte retrospectivo com todos os trabalhadores de uma empresa de petróleo na Região Norte-Nordeste do Brasil entre 2007 e 2009. A variável resposta utilizada para representar o absenteísmo com licença médica foi a incidência média de faltas com licenças médicas no período, definida pela razão entre o total de dias de licenças médicas e os dias potencialmente trabalháveis no período. Análise de regressão logística foi utilizada para investigar a associação entre incidência média de faltas > 5,0% no período e as variáveis sexo, cargo, idade, tempo de atuação, regime de trabalho, tabagismo, hipertensão arterial, índice de massa corporal, atividade física, risco coronariano, sono, glicemia, diabetes não controlado, doença do aparelho cardiovascular, digestivo, aparelho locomotor, neurológica, neoplasia, posturas forçadas no trabalho, satisfação com o trabalho, relacionamento com a chefia e atenção concentrada no trabalho. RESULTADOS : A incidência média de faltas com licenças médicas > 5,0% no período da coorte foi 15,5%. O modelo logístico revelou que trabalhadores com incidência média de faltas > 5,0% tiveram 2,6 vezes mais chance de ser do sexo feminino; 2,0 vezes mais chance de ser fumante; 1,8 vez mais chance de ser ex-fumante, 2,2 vezes mais chance de relatar sono anormal e 10,5 vezes mais chance de estarem insatisfeitos com o trabalho do que trabalhadores com incidência média de faltas ≤ 5,0% no período. CONCLUSÕES : Sexo feminino, ser fumante ou ex-fumante, estar insatisfeito com o trabalho e relatar sono anormal são bons preditores de absenteísmo ao trabalho por doença.

  17. Absenteeism at a Medium Size Philanthropic Hospital Absenteísmo em Hospital Filantrópico de Médio Porte

    Ilidia Terezinha Martelli Takahashi

    2011-06-01

    Full Text Available The absenteeism is a complex administrative problem for organizations. In hospitals, it becomes even more complicated as the patient care can not be interrupted. The term absenteeism - disease, refers to the time off work due to health problems. It is intended to analyze the absence of workers due to health problems is related to age, sex, length of employment at the institution, part work shift and functional category. This is a retrospective, descriptive and documentary study. Data was collected from medical certificates submitted in the period from 01 July 2007 to 30 June 2008 to the sector of human resources of a medium-sized philanthropic hospital. The analysis results show that the number of certificates is greater in females (90.80% and staff that work less time in the institution (61.19%. It was found that the number of certificates is higher in workers who make the rotation between morning and afternoon shifts. It was also noted that workers aged over 48 years, had lower number of certificates than the younger ones. The nursing technicians assistant were the professional categories that had presented more certificates (46.05%. The results identify several factors related to absenteeism and provide subsidies to strengthen actions to monitor the health of workers and also the implementation of actions aimed at improving the quality of life at work. O absenteísmo é um complexo problema administrativo para as organizações. Nos hospitais, torna-se ainda mais complicado, pois o cuidado ao cliente não pode ser interrompido. Entende-se por absenteísmo-doença, as faltas ao trabalho por problemas de saúde. Tem-se como objetivo analisar as faltas dos trabalhadores por causas de saúde relacionando-as com idade, sexo, tempo de trabalho na instituição, turno de trabalho e categoria funcional. Trata-se de um estudo retrospectivo, descritivo edocumental. Os dados foram coletados dos atestados de saúde apresentados no período de 01 julho de

  18. Familia y consumo de sustancias psicoactivas: una búsqueda de lo ausente Family and psychoactive substance use: A search of the absent

    María Nelcy Muñoz Astudillo

    2012-09-01

    Full Text Available Objetivo principal. Describir la relación que establecen jóvenes universitarios entre familia y consumo de sustancias psicoactivas. Metodología: Estudio de teoría fundamentada, realizado en el 2009 en Pereira, Risaralda, Colombia. 70 estudiantes universitarios participaron voluntariamente, conformaron nueve grupos focales. La información fue grabada y transcrita. Muestreo teórico. Codificación y categorización con Atlas.ti. Resultados principales: La teoría sustantiva "Familia y consumo de sustancias psicoactivas: Una búsqueda de lo ausente" se construyó con la integración de seis conceptos que permitieron develar lo ausente en las relaciones directas e indirectas. Directas: entorno familiar protector, ejercicio de autoridad, cumplimiento de tareas pedagógicas. Indirectas: conocimiento del joven y su contexto psicosocial, conocimiento de las sustancias, formación en derechos humanos. Conclusiones: Se plantean retos para el cuidado de las dinámicas familiares, en términos de seguimiento de tensiones y cadencias, buscando posibilidades de intervención desde sus propios escenarios cotidianos.Objective: describe the established relationship between university students and their families with consumption of psychoactive substance consumption (PSC. Methods: A grounded theory approach (GP model, made in 2009 in Pereira, Risaralda, Colombia. 70 university student volunteers subdivided in nine focus groups. The information was recorded and transcribed. Theoretical sampling with Atlas.ti coding and categorization. Results: The substantive theory "Family and psychoactive substance use: A search of the absent" was built on the integration of six concepts that allowed to reveal what is absent in the direct and indirect. Direct: protective family environment, exercise of authority and compliance with educational tasks. Hints: knowledge of the girl and her psychosocial context, knowledge of chemicals and human rights training. Conclusions: new

  19. Rendered invisible? The absent presence of egg providers in U.K. debates on the acceptability of research and therapy for mitochondrial disease.

    Haimes, Erica; Taylor, Ken

    2015-12-01

    Techniques for resolving some types of inherited mitochondrial diseases have recently been the subject of scientific research, ethical scrutiny, media coverage and regulatory initiatives in the UK. Building on research using eggs from a variety of providers, scientists hope to eradicate maternally transmitted mutations in mitochondrial DNA by transferring the nuclear DNA of a fertilised egg, created by an intending mother at risk of transmitting mitochondrial disease, and her male partner, into an enucleated egg provided by another woman. In this article we examine how egg providers for mitochondrial research and therapy have been represented in stakeholder debates. A systematic review of key documents and parliamentary debates shows that the balance of consideration tilts heavily towards therapeutic egg providers; research egg providers have been ignored and rendered invisible. However, mapping the various designations of therapeutic egg providers shows that their role is so heavily camouflaged that they have only an absent presence in discussions. We explore this puzzling ambivalence towards egg providers whose contributions are necessary to the success of current mitochondrial research and proposed therapies. We suggest that labels that diminish the contributions of egg providers serve certain governance objectives in managing possible future claims about, and by, therapeutic egg providers. We demonstrate that the social positioning of research egg providers is entangled within that of therapeutic egg providers which means that the former can also never receive their due recognition. This article contributes to the wider literature on the governance of new technological interventions. PMID:26712608

  20. Don't it make my brown eyes blue: co-witness misinformation about a target's appearance can impair target-absent line-up performance.

    Zajac, Rachel; Henderson, Nicola

    2009-04-01

    Misinformation from another witness has been shown to impair eyewitness reports, but little is known about how it may influence eyewitness identification. In Experiment 1, adult pairs comprising one participant and one experimental confederate viewed a video clip of a staged theft. Half of the participants were then misinformed by the confederate that the thief's accomplice had blue eyes (in fact, they were brown). Next, individual participants described the accomplice and completed a target-absent photographic line-up task comprising blue-eyed members. Misinformed participants were several times more likely than controls to describe the accomplice as having blue eyes, and twice as likely to identify someone from the line-up. In Experiment 2, when line-up members' eye colour was digitally altered from blue to brown, the line-up effect disappeared, suggesting that the increase in identifications in Experiment 1 was not a generalised increase in willingness to choose from the line-up. In Experiment 3, we discounted the possibility that discussion alone could account for the line-up misinformation effect, by subjecting all participants to co-witness discussion. PMID:19156565

  1. Double point modified analogs of vitamin d as potent activators of vitamin D receptor.

    Nadkarni, Sharmin; Chodynski, Michal; Corcoran, Aoife; Marcinkowska, Ewa; Brown, Geoffrey; Kutner, Andrzej

    2015-01-01

    Rational design, chemical synthesis, structural analysis, molecular modeling and biological evaluation are reviewed for all the double point modified vitamin D analogs that have been developed as potential therapeutics over the last several years. The idea of double modifications was based on the 3D structure of the ligand binding domain of the model of the vitamin D receptor. It was recently proved that structural modifications in the two remote parts of the vitamin D molecule might have additive biological effects resulting in an increased functional activity and lowered calcemic side effect. Recent in vivo experiments clearly demonstrated the potential use of these analogs in new therapeutic areas such as autoimmune and hyper-proliferative diseases, including cancer and the systemic treatment of psoriasis. Although some of these analogs are already approaching clinical trials, the molecular mechanism of action and their improved efficiency still remain to be fully understood. In this review the key steps of the convergent synthetic strategies that combine the modified A-ring and the CD-ring fragment carrying the altered side-chain are presented. The advantages of using the natural alicyclic and acyclic precursors are demonstrated as well as all the modern synthetic methodologies used for combining structural fragments. The results of molecular mechanics modeling are critically examined as well as the advantages and limitations of the use of the models of vitamin D proteins for the docking experiments and the design of new analogs. The potential use of advanced structural approaches, including high resolution X-ray crystallography, is discussed as to the prospect of providing a better understanding of the observed activity of modified analogs. Biological profiles in vitro and in vivo for groups of analogs are presented in a new tabular form to illustrate structure activity relationships. PMID:25483861

  2. Absent otoacoustic emissions predict otitis media in young Aboriginal children: A birth cohort study in Aboriginal and non-Aboriginal children in an arid zone of Western Australia

    Stokes Annette

    2008-08-01

    Full Text Available Abstract Background Otitis media (OM is the most common paediatric illness for which antibiotics are prescribed. In Australian Aboriginal children OM is frequently asymptomatic and starts at a younger age, is more common and more likely to result in hearing loss than in non-Aboriginal children. Absent transient evoked otoacoustic emissions (TEOAEs may predict subsequent risk of OM. Methods 100 Aboriginal and 180 non-Aboriginal children in a semi-arid zone of Western Australia were followed regularly from birth to age 2 years. Tympanometry was conducted at routine field follow-up from age 3 months. Routine clinical examination by an ENT specialist was to be done 3 times and hearing assessment by an audiologist twice. TEOAEs were measured at ages Results At routine ENT specialist clinics, OM was detected in 55% of 184 examinations in Aboriginal children and 26% of 392 examinations in non-Aboriginal children; peak prevalence was 72% at age 5–9 months in Aboriginal children and 40% at 10–14 months in non-Aboriginal children. Moderate-severe hearing loss was present in 32% of 47 Aboriginal children and 7% of 120 non-Aboriginal children aged 12 months or more. TEOAE responses were present in 90% (46/51 of Aboriginal children and 99% (120/121 of non-Aboriginal children aged Overall prevalence of type B tympanograms at field follow-up was 50% (n = 78 in Aboriginal children and 20% (n = 95 in non-Aboriginal children. Conclusion The burden of middle ear disease is high in all children, but particularly in Aboriginal children, one-third of whom suffer from moderate-severe hearing loss. In view of the frequently silent nature of OM, every opportunity must be taken to screen for OM. Measurement of TEOAEs at age 1–2 months to identify children at risk of developing OM should be evaluated in a routine health service setting.

  3. Lower-frequency event-related desynchronization: a signature of late mismatch responses to sounds, which is reduced or absent in children with specific language impairment

    Bishop, D V M; Hardiman, Mervyn J.; Barry, Johanna G.

    2010-01-01

    Poor discrimination of non-linguistic sounds has been implicated in language-learning problems in children, but research evidence has been inconsistent. This study included 32 participants with specific language impairment (SLI) and 32 typically-developing controls aged 7 to 16 years. Frequency discrimination thresholds were estimated in a task where participants had to distinguish a higher-frequency tone from a 1000 Hz tone. Neurophysiological responses were assessed in an oddball paradigm. Stimuli were either (a) 1030- or 1200-Hz pure tones (deviants) presented in a series of standard 1000-Hz tones, or (b) syllables: deviant [da] or [bi] in a series of standard [ba]. On the behavioural task, children (7 to 11-year-olds) had high thresholds, regardless of language status, but teenagers (12-16 yr) with SLI had higher thresholds than their controls. Conventional analysis of electrophysiological responses showed no difference between groups for the mismatch negativity (MMN), but the late discriminative negativity (LDN) was reduced in amplitude for smaller deviants in participants with SLI. Time-frequency analysis revealed that whereas the MMN reflected enhanced inter-trial coherence in the theta frequency band, the LDN corresponded to a period of event-related desynchronization extending across a wide low-frequency band including delta, theta and alpha. This manifested as a drop in power in those frequencies, which was marked in the controls but reduced or absent in children with SLI across all stimulus types. This provides compelling evidence for a low-level auditory perceptual impairment in SLI that affects a processing stage after initial detection of a sound change. PMID:21084613

  4. Epigenetic Silencing of Eyes Absent 4 Gene by Acute Myeloid Leukemia 1-Eight-twenty-one Oncoprotein Contributes to Leukemogenesis in t(8;21) Acute Myeloid Leukemia

    Huang, Sai; Jiang, Meng-Meng; Chen, Guo-Feng; Qian, Kun; Gao, Hong-Hao; Guan, Wei; Shi, Jin-Long; Liu, An-Qi; Liu, Jing; Wang, Bian-Hong; Li, Yong-Hui; Yu, Li

    2016-01-01

    Background: The acute myeloid leukemia 1 (AML1)-eight-twenty-one (ETO) fusion protein generated by the t(8;21)(q22;q22) translocation is considered to display a crucial role in leukemogenesis in AML. By focusing on the anti-leukemia effects of eyes absent 4 (EYA4) gene on AML cells, we investigated the biologic and molecular mechanism associated with AML1-ETO expressed in t(8;21) AML. Methods: Qualitative polymerase chain reaction (PCR), quantitative reverse transcription PCR (RT-PCR), and Western blotting analysis were used to observe the mRNA and protein expression levels of EYA4 in cell lines. Different plasmids (including mutant plasmids) of dual luciferase reporter vector were built to study the binding status of AML1-ETO to the promoter region of EYA4. Chromatin immunoprecipitation assay was used to study the epigenetic silencing mechanism of EYA4. Bisulfite sequencing was applied to detect the methylation status in EYA4 promoter region. The influence of EYA4 gene in the cell proliferation, apoptosis, and cell clone-forming ability was detected by the technique of Cell Counting Kit-8, flow cytometry, and clonogenic assay. Results: EYA4 gene was hypermethylated in AML1-ETO+ patients and its expression was down-regulated by 6-fold in Kasumi-1 and SKNO-1 cells, compared to HL-60 and SKNO-1-siA/E cells, respectively. We demonstrated that AML1-ETO triggered the epigenetic silencing of EYA4 gene by binding at AML1-binding sites and recruiting histone deacetylase 1 and DNA methyltransferases. Enhanced EYA4 expression levels inhibited cellular proliferation and suppressed cell colony formation in AML1-ETO+ cell lines. We also found EYA4 transfection increased apoptosis of Kasumi-1 and SKNO-1 cells by 1.6-fold and 1.4-fold compared to negative control, respectively. Conclusions: Our study identified EYA4 gene as targets for AML1-ETO and indicated it as a novel tumor suppressor gene. In addition, we provided evidence that EYA4 gene might be a novel therapeutic target

  5. Positive response to neoadjuvant cyclophosphamide and doxorubicin in topoisomerase II nonamplified/HER2/neu negative/polysomy 17 absent breast cancer patients

    Henry G Kaplan

    2010-08-01

    Full Text Available Henry G Kaplan1, Judith A Malmgren2,3, Mary Atwood1, Lynn C Goldstein41Swedish Cancer Institute at Swedish Medical Center, Seattle, Washington, USA; 2HealthStat Consulting Inc., Seattle, Washington, USA; 3School of Public Health and Community Medicine, Department of Epidemiology, University of Washington, Seattle, Washington, USA; 4PhenoPath Laboratories, Seattle, Washington, USAPurpose: Human epidermal growth factor receptor 2 (HER2/neu, topoisomerase II alpha (TOP2A, and polysomy 17 may predict tumor responsiveness to doxorubicin (DOX therapy.Methods: We identified neoadjuvant DOX/cyclophosphamide treated breast cancer patients in our registry from 1997 to 2008 with sufficient tissue for testing (n = 34. Fluorescence in situ hybridization (FISH testing was done on deparaffinized tissue sections pretreated using vendor’s standard protocol modification, and incubated with US Food and Drug Administration approved Abbott Diagnostics Vysis PathVysion™ probe set, including Spectrum-Green-conjugated probe to a-satellite DNA located at the centromere of chromosome 17 (17p11.1–q11.1 and a Spectrum-Orange-conjugated probe to the TOP2A gene. Morphometric analysis was performed using a MetaSystems image analysis system. Manual counting was performed on all samples in which autofluorescence and/or artifact prevented the counting of sufficient numbers of cells. A ratio >2.0 was considered positive for TOP2A amplification. Polysomy 17 (PS17 presence was defined as signals of ≥2.5. Outcomes were pathological complete response (pCR, partial response (PR, and nonresponse (NR.Results: Of 34 patients tested, one was TOP2A amplified (hormone receptor negative/HER2 ­negative, partial responder. The subset of TOP2A nonamplified, HER2 negative, and PS17 absent (n = 23 patients had treatment response: pCR = 2 (9%, PR = 14 (61%, and NR = 7 (30%. Including the two PS17 present and HER2-positive patients (n = 33, 76% of TOP2A nonamplified patients had pCR or PR

  6. Absent in Melanoma 2 (AIM2) is an important mediator of interferon-dependent and -independent HLA-DRA and HLA-DRB gene expression in colorectal cancers.

    Lee, J; Li, L; Gretz, N; Gebert, J; Dihlmann, S

    2012-03-01

    Absent in Melanoma 2 (AIM2) is a member of the HIN-200 family of hematopoietic, IFN-inducible, nuclear proteins, associated with both, infection defense and tumor pathology. Recently, AIM2 was found to act as a DNA sensor in innate immunity. In addition, we and others have previously demonstrated a high frequency of AIM2-alterations in microsatellite unstable (MSI-H) tumors. To further elucidate AIM2 function in colorectal tumors, we here addressed AIM2-responsive target genes by microarray based gene expression profiling of 22 244 human genes. A total of 111 transcripts were significantly upregulated, whereas 80 transcripts turned out to be significantly downregulated in HCT116 cells, constitutively expressing AIM2, compared with AIM2-negative cells. Among the upregulated genes that were validated by quantitative PCR and western blotting we recognized several interferon-stimulated genes (ISGs: IFIT1, IFIT2, IFIT3, IFI6, IRF7, ISG15, HLA-DRA, HLA-DRB, TLR3 and CIITA), as well as genes involved in intercellular adhesion and matrix remodeling. Expression of ISGs correlated with expression of AIM2 in 10 different IFN-γ treated colorectal cancer cell lines. Moreover, small interfering RNA-mediated knock-down of AIM2 resulted in reduced expression of HLA-DRA, HLA-DRB and CIITA in IFN-γ-treated cells. IFN-γ independent induction of HLA-DR genes and their encoded proteins was also demonstrated upon doxycyclin-regulated transient induction of AIM2. Luciferase reporter assays revealed induction of the HLA-DR promoter upon AIM2 transfection in different cell lines. STAT-signaling was not involved in IFN-γ independent induction of ISGs, arguing against participation of cytokines released in an autostimulating manner. Our data indicate that AIM2 mediates both IFN-γ dependent and independent induction of several ISGs, including genes encoding the major histocompatibility complex (MHC) class II antigens HLA-DR-α and -β. This suggests a novel role of the IFN/AIM2/ISG

  7. Skin vasoreactivity to insulin iontophoresis is reduced in elderly subjects and is absent in treated non-insulin-dependent diabetes patients.

    Rossi, Marco; Cupisti, Adamasco; Ricco, Roberto; Santoro, Gino; Pentimone, Ferdinando; Carpi, Angelo

    2004-12-01

    subjects and show that this effect is reduced by aging and is absent in treated NIDDM patients. The local skin vasodilatatory effect induced by insulin seems to involve mechanisms different from those underlying the skin postischemic hyperemia. PMID:15589063

  8. A Report to the President of the United States [from the] Intergovernmental Advisory Council on Education, National Networking Conference [on] The Absent Parent (Washington, D.C. May 9-10, 1988).

    Intergovernmental Advisory Council on Education (ED), Washington, DC.

    This report on child care and the Federal government's role in child care is based on the National Networking Conference on the Absent Parent which was held May 9-10, 1988 under the sponsorship of the President's Intergovernmental Advisory Council on Education (IACE). The report begins with 12 recommendations concerning day care and latchkey…

  9. Activities.

    Moody, Mally

    1992-01-01

    A series of four activities are presented to enhance students' abilities to appreciate and use trigonometry as a tool in problem solving. Activities cover problems applying the law of sines, the law of cosines, and matching equivalent trigonometric expressions. A teacher's guide, worksheets, and answers are provided. (MDH)

  10. Vector-averaged gravity-induced changes in cell signaling and vitamin D receptor activity in MG-63 cells are reversed by a 1,25-(OH)2D3 analog, EB1089

    Narayanan, R.; Smith, C. L.; Weigel, N. L.

    2002-01-01

    Skeletal unloading in an animal hindlimb suspension model and microgravity experienced by astronauts or as a result of prolonged bed rest causes site-specific losses in bone mineral density of 1%-2% per month. This is accompanied by reductions in circulating levels of 1,25-(OH)(2)D(3), the active metabolite of vitamin D. 1,25-(OH)(2)D(3), the ligand for the vitamin D receptor (VDR), is important for calcium absorption and plays a role in differentiation of osteoblasts and osteoclasts. To examine the responses of cells to activators of the VDR in a simulated microgravity environment, we used slow-turning lateral vessels (STLVs) in a rotating cell culture system. We found that, similar to cells grown in microgravity, MG-63 cells grown in the STLVs produce less osteocalcin, alkaline phosphatase, and collagen Ialpha1 mRNA and are less responsive to 1,25-(OH)(2)D(3). In addition, expression of VDR was reduced. Moreover, growth in the STLV caused activation of the stress-activated protein kinase pathway (SAPK), a kinase that inhibits VDR activity. In contrast, the 1,25-(OH)(2)D(3) analog, EB1089, was able to compensate for some of the STLV-associated responses by reducing SAPK activity, elevating VDR levels, and increasing expression of osteocalcin and alkaline phosphatase. These studies suggest that, not only does simulated microgravity reduce differentiation of MG-63 cells, but the activity of the VDR, an important regulator of bone metabolism, is reduced. Use of potent, less calcemic analogs of 1,25-(OH)(2)D(3) may aid in overcoming this defect. Copyright 2002 Elsevier Science Inc.

  11. Behavioral insensitivity to restraint stress, absent fear suppression of behavior and impaired spatial learning in transgenic rats with hippocampal neuropeptide Y overexpression

    Thorsell, A.; Michalkiewicz, M.; Dumont, Y.; Quirion, R.; Caberlotto, L.; Rimondini, R.; Mathé, A. A.; Heilig, M.

    2000-01-01

    Exogenous neuropeptide Y (NPY) reduces experimental anxiety in a wide range of animal models. The generation of an NPY-transgenic rat has provided a unique model to examine the role of endogenous NPY in control of stress and anxiety-related behaviors using paradigms previously used by pharmacological studies. Locomotor activity and baseline behavior on the elevated plus maze were normal in transgenic subjects. Two robust phenotypic traits were observed. (i) Transge...

  12. The Causal Effect of Vitamin D Binding Protein (DBP) Levels on Calcemic and Cardiometabolic Diseases: A Mendelian Randomization Study

    Leong, Aaron; Rehman, Waheed; Dastani, Zari; Greenwood, Celia; Timpson, Nicholas; Langsetmo, Lisa; Berger, Claudie; ,; Fu, Lei; Wong, Betty Y. L.; Malik, Suneil; Malik, Rainer; Hanley, David A; Cole, David E.C.; Goltzman, David

    2014-01-01

    Editors' Summary Background Vitamin D deficiency is an increasingly common public health concern. According to some estimates, more than a billion people worldwide may be vitamin D deficient. Indeed, many people living in the US and Europe (in particular, elderly people, breastfed infants, people with dark skin, and obese individuals) have serum (circulating) 25-hydroxy-vitamin D (25OHD) levels below 50 nmol/l, the threshold for vitamin D deficiency. Vitamin D helps the body absorb calcium, a...

  13. Shadows of an absent partner: ATP hydrolysis and phosphoenzyme turnover of the Spf1 (sensitivity to Pichia farinosa killer toxin) P5-ATPase.

    Corradi, Gerardo R; de Tezanos Pinto, Felicitas; Mazzitelli, Luciana R; Adamo, Hugo P

    2012-08-31

    The P5-ATPases are important components of eukaryotic cells. They have been shown to influence protein biogenesis, folding, and transport. The knowledge of their biochemical properties is, however, limited, and the transported ions are still unknown. We expressed in Saccharomyces cerevisiae the yeast Spf1 P5A-ATPase containing the GFP fused at the N-terminal end. The GFP-Spf1 protein was localized in the yeast endoplasmic reticulum. Purified preparations of GFP-Spf1 hydrolyzed ATP at a rate of ~0.3-1 μmol of P(i)/mg/min and formed a phosphoenzyme in a simple reaction medium containing no added metal ions except Mg(2+). No significant differences were found between the ATPase activity of GFP-Spf1 and recombinant Spf1. Omission of protease inhibitors from the purification buffers resulted in a high level of endogenous proteolysis at the C-terminal portion of the GFP-Spf1 molecule that abolished phosphoenzyme formation. The Mg(2+) dependence of the GFP-Spf1 ATPase was similar to that of other P-ATPases where Mg(2+) acts as a cofactor. The addition of Mn(2+) to the reaction medium decreased the ATPase activity. The enzyme manifested optimal activity at a near neutral pH. When chased by the addition of cold ATP, 90% of the phosphoenzyme remained stable after 5 s. In contrast, the phosphoenzyme rapidly decayed to less than 20% when chased for 3 s by the addition of ADP. The greater effect of ADP accelerating the disappearance of EP suggests that GFP-Spf1 accumulated the E1~P phosphoenzyme. This behavior may reflect a limiting countertransported substrate needed to promote turnover or a missing regulatory factor. PMID:22745129

  14. Absenteísmo na enfermagem: uma revisão integrativa Absentismo en enfermería: una revisión integrativa Absenteeism in nursing: an integrative review

    Michele Cristiene Nachtigall Barboza Martinato

    2010-03-01

    Full Text Available O absenteísmo na enfermagem é um problema de extrema preocupação, o qual interfere no cuidado ao cliente, sobrecarrega os demais integrantes da equipe e pode levar ao adoecimento. Objetiva-se neste estudo conhecer as produções científicas nacionais, resumos de artigos, publicados no período de 2003 a 2008, abrangendo o tema absenteísmo dos profissionais de enfermagem. Realizou-se uma busca on-line, nas bases de dados: LILACS, SciELO, BDENF e Portal CAPES. Da análise emergiram dois temas: Doenças que geram o absenteísmo nos profissionais de enfermagem; e Estratégias criadas para minimizar o absenteísmo. Concluiu-se que os profissionais de enfermagem são acometidos por inúmeros problemas de saúde, sendo uma das causas do absenteísmo. Destacaram-se as estratégias do dimensionamento de pessoal e as ações preventivas como modos de estabelecerem melhores condições de trabalho, minimizando o absenteísmo.El absentismo en la enfermería es un asunto de extrema preocupación, interferir con el cuidado del cliente, abruma a los otros miembros del equipo y puede conducir a la enfermedad. El objetivo de este estudio fue conocer la producción científica nacional, los resúmenes de artículos publicados desde 2003 hasta 2008, que abarca el tema de ausentismo de los profesionales de enfermería. Llevó a cabo una búsqueda en línea, en bases de datos: LILACS, SciELO, BDENF y el Portal de la CAPES. El análisis surgieron dos temas: Las enfermedades que generan en el ausentismo de los profesionales de enfermería, y las estrategias destinadas a reducir el absentismo. Llegó a la conclusión de que las enfermeras se ven afectados por numerosos problemas de salud, una de las causas de ausentismo. Destacó las estrategias para el diseño de las acciones personales y de prevención como la manera de establecer mejores condiciones de trabajo, reduciendo al mínimo el absentismo.Absenteeism in nursing staff is a matter of great concern. It

  15. Prevalência de dismenorreia em universitárias e sua relação com absenteísmo escolar, exercício físico e uso de medicamentos

    Janaina Mayer de Oliveira Nunes

    2013-09-01

    Full Text Available Objetivo: Verificar a prevalência de dismenorreia em universitárias e a frequência de absenteísmo escolar, prática de exercícios físicos e utilização de medicamentos para tratamento dessa síndrome. Métodos: Trata-se de um estudo transversal realizado com jovens universitárias por meio de um questionário autoaplicado contendo dados sociodemográficos, obstétricos e ginecológicos. Avaliou-se a dor menstrual através da escala visual analógica (EVA. Para análise descritiva dos dados, foram utilizadas frequência, percentagem, média e desvio padrão. Resultados: A amostra constou de 130 mulheres, com idade entre 17 e 33 anos (20,6±2,7 anos. Cento e vinte quatro voluntárias (95,4% queixaram-se de dismenorreia. Quanto à sua intensidade, a maioria sentia dor menstrual moderada ou grave (51,6% e 36,3%, respectivamente. Sessenta (48,4% participantes referiram absenteísmo escolar devido à dor menstrual; dessas, nenhuma tinha dor leve. Dentre as voluntárias que apresentavam dismenorreia moderada e grave, apenas 24 (20,2% praticavam exercício físico e a maioria delas (79% necessitava utilizar fármacos para tratar essa síndrome. Conclusão:A dismenorreia teve alta prevalência entre as universitárias avaliadas e na maior parte dos casos se apresentou com intensidade limitante, levando ao absenteísmo escolar. A maioria das mulheres com dismenorreia de intensidades moderada e grave não pratica exercício físico e necessita utilizar medicamentos para tratar essa síndrome.

  16. Recurring Candida albicans esophagitis in a HIV-infected patient undergoing long-Term antiretroviral therapy, and with absent-negligible immunodeficiency

    Roberto Manfredi

    2007-12-01

    Full Text Available A patient with HIV infection developed the first episode of AIDS-defining opportunism (severe Candida albicans esophagitis with an underlying CD4+ lymphocyte count of 1,025 cells/µL. After treatment with a highly active antiretroviral therapy (HAART, taken with insufficient compliance and leaving a residual viral load, our patient suffered from two relapses of esophageal candidiasis, which occurred three months and seven years later, when his CD4+ lymphocyte count was 930 and 439 cells/µL, respectively, and a viral load slightly above 10(4 copies/mL was still present. Also in the HAART era, Candida esophagitis remains one of the most common AIDS-defining diseases, but a presentation with a concurrent CD4+ count above 1,000 cells/µL remains a rare exception, as well as the two isolated, subsequent relapses, occurred with a CD4+ count ranging from 439 to 930 cells/µL, and a residual HIV viremia due to insufficient adherence to the prescribed HAART regimens. Our case report represents the opportunity to revisit the epidemiology and, especially, the pathogenesis of this opportunistic fungal complication in HIV-infected patients and in other subjects at risk, on the ground of an extensive literature review, and to explore possible alternative supporting factors other than the crude absolute CD4+ lymphocyte count, with emphasis on the possible role of a persisting HIV viremia, and other potential contributing factors. Clinicians engaged with immunocompromised patients and subjects with HIV disease, should be aware that a Candida esophagitis may occur and relapse also when the cell-mediated immunity, as measured by a simple CD4+ cell count, do not show relevant abnormalities.

  17. Do Dysphagic Patients with an Absent Pharyngeal Swallow Have a Shorter Survival than Dysphagic Patients with Pharyngeal Swallow? Prognostic Importance of a Therapeutic Videoradiographic Swallowing Study (TVSS)

    Buelow, M.; Olsson, R.; Ekberg, O. [Malmoe Univ. Hospital (Sweden). Dept. of Diagnostic Radiology

    2005-04-01

    therapeutic strategies were recommended compared to the WPS group. In the WPS group, diet modification was frequent. Several patients had different therapeutic strategies. At the end of the study, 8/40 patients (20%) in the APS group had recovered and regained the ability to elicit the pharyngeal stage of swallow. All eight had achieved active swallowing rehabilitation.

  18. Study on the absenteeism of nursing professionals in a psychiatric center in Manaus, Brazil Estudio sobre ausentismo en profesionales de enfermería del centro psiquiátrico de Manaus, Brasil Estudo do absenteísmo dos profissionais de enfermagem de um centro psiquiátrico em Manaus, Brasil

    Sandra Greice Becker

    2008-02-01

    Full Text Available This quantitative study was performed to research the rate of absenteeism of nursing professionals in a psychiatric center in Manaus, from January/2004 to January/2005, in the Human Resources sector of the institution. In this period, the records of workers who were absent from their professional activities for at least one day were checked. Results showed that there were 415 absence reports in the nursing team during the studied period, by 74.29% of nursing professionals. The average rate of absenteeism is 2.79. The main reason reported for absenteeism was illness. These data suggest further studies are needed in order to improve professionals' health, allowing for better quality of life, and, consequently, for providing better health care to Single Health System users.Con el objetivo de investigar sobre el índice de ausentismo en trabajadores de enfermería del Centro Psiquiátrico de Manaus, se realizó una investigación cuantitativa durante el periodo de enero de 2004 a enero del 2005 en el sector de recursos humanos de la institución en mención. Durante este periodo se buscó a través de consulta documental, registros de los trabajadores que tuvieron faltas de por lo menos 1 día en sus actividades laborales. Los resultados mostraron que durante el periodo de la investigación, hubo 415 registros de faltas por parte del equipo de enfermería, totalizando 74,29%. El índice del ausentismo profesional fue en promedio 2.79. Se observó que el principal motivo del ausentismo fue por enfermedad. Este hecho resalta la necesidad de mejorar la salud del trabajador, favoreciendo a una mejor calidad de vida en el trabajo y en consecuencia una mejor atención a la salud del usuario a través del SUS.Com o objetivo de investigar o índice de absenteísmo dos trabalhadores de enfermagem de um Centro Psiquiátrico em Manaus, realizou-se esta pesquisa quantitativa, no período de janeiro de 2004 a janeiro de 2005, no setor de recursos humanos da

  19. Fatores relacionados ao absenteísmo por doença em profissionais de enfermagem Factors related to sickness absenteeism among nursing personnel

    Ricardo José dos Reis

    2003-10-01

    Full Text Available OBJETIVO: Analisar os afastamentos de curta duração de profissionais de enfermagem de um hospital universitário. MÉTODOS: A população estudada foi de 965 profissionais de enfermagem de um hospital universitário e que estavam em atividade em 1º de janeiro de 2000.Foram analisados afastamentos do trabalho desse grupo por até 30 dias em um ano civil. Foi feita a descrição dos afastamentos por doença (agregados em um ano, da demanda pelo serviço e dos diagnósticos. Por meio de análise multivariável foi estimado o risco relativo utilizando a distribuição de erro binomial negativa. RESULTADOS: Cerca de 65% dos trabalhadores geraram 1.988 consultas, das quais 68,6% resultaram em afastamento do trabalho. Os grupos com maior demanda foram técnicos de enfermagem, mulheres e estatutários (OR=1,61; 1,47; 1,53 respectivamente. Os diagnósticos mais freqüentes foram os relacionados ao aparelho respiratório. Para afastamentos, não foram encontradas diferenças para gênero e idade. Pelo menos um afastamento foi concedido a 57,6% da população, o que corresponde a 87,8% dos trabalhadores atendidos. Estes geraram um total de 1.364 afastamentos, 1,41 por trabalhador e 5.279 dias perdidos. A análise multivariável, ao considerar separadamente os sexos, mostrou efeito apenas do vínculo empregatício (RR=1,45 e RR=2,43 para mulheres e homens. CONCLUSÕES: Existe relação entre afastamento e vínculo empregatício. Faz-se necessário incluir outras variáveis, como tempo na empresa, turno e carga reprodutiva, em futuras pesquisas.OBJECTIVE: To analyze short-term leaves among the healthcare staff of a university hospital. METHODS: Study population included 965 nursing professionals active on 1 January 2000. Leaves up to 30 days in one year were analyzed. Sickness leaves (one-year aggregate, service demand, and diagnoses were described. Relative risk was estimated through multivariate analysis, using negative binomial distribution. RESULTS

  20. Classical conditioned responses to absent tones

    Häusler Udo; Jürgens Uwe; Bangert Marc; Altenmüller Eckart

    2006-01-01

    Abstract Background Recent evidence for a tight coupling of sensorimotor processes in trained musicians led to the question of whether this coupling extends to preattentively mediated reflexes; particularly, whether a classically conditioned response in one of the domains (auditory) is generalized to another (tactile/motor) on the basis of a prior association in a second-order Pavlovian paradigm. An eyeblink conditioning procedure was performed in 17 pianists, serving as a model for overlearn...

  1. Ras activation by SOS

    Iversen, Lars; Tu, Hsiung-Lin; Lin, Wan-Chen;

    2014-01-01

    SOS molecules catalyzing nucleotide exchange in H-Ras. Single-molecule kinetic traces revealed that SOS samples a broad distribution of turnover rates through stochastic fluctuations between distinct, long-lived (more than 100 seconds), functional states. The expected allosteric activation of SOS by...... Ras-guanosine triphosphate (GTP) was conspicuously absent in the mean rate. However, fluctuations into highly active states were modulated by Ras-GTP. This reveals a mechanism in which functional output may be determined by the dynamical spectrum of rates sampled by a small number of enzymes, rather...

  2. Dor dentária como motivo de absenteísmo em uma população de trabalhadores Dental pain as the motive for absenteeism in a sample of workers

    Maria Helena Monteiro de Barros Miotto

    2012-05-01

    Full Text Available O objetivo foi analisar a prevalência da dor dentária e a ocorrência de absenteísmo provocado por dor de dente e as possíveis associações com características sociodemográficas. Um estudo analítico de corte transversal foi realizado em uma amostra aleatória de 170 sujeitos de um universo de 545 funcionários da Prefeitura Municipal de Venda Nova do Imigrante (ES. Para a coleta de dados foi utilizado um roteiro de 27 itens aplicado por uma servidora municipal. Para comparação dos percentuais entre dor dentária e absenteísmo com os fatores sociodemográficos foi utilizado o teste exato de Fisher. A prevalência de dor dentária entre os trabalhadores foi de 43% e a de absenteísmo por dor dentária foi de 23,4%. Funcionários com grau de escolaridade até ensino médio incompleto relataram uma chance duas vezes maior de dor de dente (OR = 2,144, IC95% = 1,141;4,030 . Trabalhadores do sexo masculino (OR = 5,714, IC95% = 1,773;18,416, de escolaridade até ensino médio incompleto (OR = 4,875, IC95% = 1,405;16,909 e renda familiar até dois salários mínimos (OR= 3,613, IC95% = 0,931;14,021 apresentaram maior prevalência de absenteísmo associada à dor de dente. A prevalência de dor encontrada demonstra a necessidade de políticas de promoção de saúde bucal para melhorar a qualidade de vida dos trabalhadores.The aim of this study was to analyze the prevalence of dental pain, absenteeism induced by dental pain, and the association with socio-demographic characteristics. The research had a cross-sectional design using a random sample of 170 municipal employees from the City of Venda Nova do Imigrante, Espírito Santo state, Brazil, selected from a universe of 545 individuals. Data was collected using a 27-item questionnaire applied by a municipal worker. The comparison of dental pain and prevalence of absenteeism with socio-demographic and functional characteristics was verified using Fisher's Exact test. The research project was

  3. Perfil do absenteísmo em um banco estatal em Minas Gerais: análise no período de 1998 a 2003 Absenteeism profile in a state bank in Minas Gerais between 1998 a 2003

    Luiz Sérgio Silva

    2008-12-01

    Full Text Available O objetivo dessa pesquisa foi estudar o perfil do absenteísmo em uma empresa bancária estatal e estimar a prevalência de afastamentos pelas doenças que mais acometeram seus trabalhadores. Foi realizado um estudo transversal, descritivo e quantitativo abordando absenteísmo e prevalência de causas de afastamento em um banco estatal no estado de Minas Gerais, no período de 1998 a 2003. Os índices de absenteísmo apresentaram distribuição heterogênea, estando em queda no período estudado, exceto a taxa de freqüência, devido a modificações nos números que compõem seus numeradores e denominadores. As prevalências das doenças osteomusculares e dos distúrbios mentais e comportamentais foram 33,25 e 22,21 afastamentos por 1.000 trabalhadores, respectivamente. Houve predomínio de afastamentos de trabalhadores do sexo feminino, com idade entre 40 e 49 anos de idade, com tempo de empresa superior a 21 anos e com funções com menores valores de remuneração. O estudo indicou que as doenças osteomusculares e do tecido conjuntivo que antes predominavam na empresa estão em queda. Houve também ascensão dos distúrbios mentais e comportamentais, indicando possível mudança no perfil de adoecimento. Mais estudos são necessários para a explicação dos resultados observados.The purpose of this paper was to study the profile of absenteeism in a state bank and to establish the more frequent causes for sick leaves among the members of the staff. A cross-cut, descriptive and quantitative study was developed for approaching absenteeism and its most frequent causes in a state bank in the state of Minas Gerais between 1998 and 2003. The absenteeism rates were homogenous, with a decrease during the period of the study, except for the frequency rates, as a result of alterations in their numerators and denominators. The prevalence of musculoskeletal diseases and mental disorders was of 33,25 and 22,21 leaves per 1,000 workers respectively

  4. Development of Family Adversity Scale for Parent-absent Children in Rural China%留守儿童家庭处境不利问卷的编制

    范兴华; 方晓义; 陈锋菊

    2011-01-01

    目的:编制适用于留守儿童的家庭处境不利问卷.方法:基于文献分析和访谈结果,形成问卷的70个初始项目.以1085名农村儿童为被试,通过因素分析、相关分析和方差分析,形成由51个项目构成的最终问卷.结果:最终问卷由缺少父母关爱、家庭气氛冷清、物质生活差、生活照顾不周、应对能力差、学习管理不善、监管不力、缺少沟通、放任、监护人心情差、命令强迫、严厉惩罚等12个维度构成;模型的拟合指数为:x2/df=2.131,NFI=0.960,RFI=0.956,IFI=0.978,TLI=0.976,CFI=0.978,RMSEA=0.046;各维度与总体幸福感、积极情感呈负相关,与消极情感、抑郁、孤独、问题行为呈正相关;不同监护类型留守儿童在各维度上的得分显著高于父母监护的一般儿童;各维度的Cron-bach α系数在0.504-0.871之间,重测信度在0.614-0.815之间.结论:自编问卷的信效度基本达到测量学要求.%Objective: To develop a Family Adversity Inventory for Parent-absent Children in Chinese rural area. Methods: 72 original items were developed based on literature analysis and interviews. The inventory was then administered to 1085 rural children from Hunan Province. Factor analysis, correlation analysis and variance analysis were conducted. Finally, 51 items were left. Results: EFA result indicated there were disadvantages in parental care, family atmosphere, material life, life care, family ability to cope with events, learning management, supervision, communication, permission, mood of primary guardian, command and coercion, harsh punishment in parent-absent children family. The fit index was as follows: tfdf=2.l31, NFI=0.960, RFI=0.956, IFI=0.978, TLI=0.976, CFI=0.978, RMSEA=0.046. 12 dimensions of family adversity were negatively correlated with total well-being, positive feeling and positively correlated to negative feeling, depression, loneliness and problem behavior. Parent-absent children in different care

  5. Abordagem multifatorial do absenteísmo por doença em trabalhadores de enfermagem Abordaje multifactorial del ausentismo por enfermedad en trabajadores de enfermería A multifactorial approach to sickness absenteeism among nursing staff

    Roberta Carolina Ferreira

    2012-04-01

    Full Text Available OBJETIVO: Analisar fatores associados ao absenteísmo por doença autorreferido em trabalhadores de enfermagem. MÉTODOS: Estudo transversal com 1.509 trabalhadores de três hospitais públicos no município do Rio de Janeiro, RJ, em 2006. O absenteísmo foi classificado em três níveis: nenhum dia, poucos dias (um a nove dias e muitos dias (> 10 dias, a partir da resposta a uma pergunta do questionário de avaliação do índice de capacidade para o trabalho. As análises de regressão logística levaram em conta um modelo conceitual com base em determinantes distais (condições socioeconômicas, de níveis intermediários I (características ocupacionais e II (características do estilo de vida, e proximais (doenças e condições de saúde. RESULTADOS: As frequências de absenteísmo por doença foram de 20,3% e 16,6% para poucos e muitos dias, respectivamente. Aqueles que referiram mais de um emprego, doenças osteomusculares e avaliaram sua saúde como ruim ou regular apresentaram chances mais elevadas de absenteísmo. Comparados aos enfermeiros, os auxiliares tiveram menor chance de referir poucos dias e os técnicos, maiores chances de apresentar muitos dias de ausência. Chances mais elevadas de referir muitos dias de ausência foram observadas entre os servidores públicos em relação aos contratados (OR = 3,12; IC95% 1,86;5,22 e entre os casados (OR = 1,73; IC95% 1,14;2,63 e separados, divorciados e viúvos (OR = 2,06; IC95% 1,27;3,35, comparados aos solteiros. CONCLUSÕES: Diferentes variáveis foram associadas às duas modalidades de absenteísmo, o que sugere sua determinação múltipla e complexa, relacionada a fatores de diversos níveis que não podem ser explicados apenas por problemas de saúde.OBJETIVO: Analizar factores asociados al ausentismo por enfermedad auto referida en trabajadores de enfermería. MÉTODOS: Estudio transversal con 1.509 trabajadores de tres hospitales públicos en Rio de Janeiro, Sureste de Brasil

  6. 1α,25(OH)2-3-Epi-Vitamin D3, a Natural Physiological Metabolite of Vitamin D3: Its Synthesis, Biological Activity and Crystal Structure with Its Receptor

    Molnár, Ferdinand; Sigüeiro, Rita; Sato, Yoshiteru; Araujo, Clarisse; Schuster, Inge; Antony, Pierre; Peluso, Jean; Muller, Christian; Mouriño, Antonio; Moras, Dino; Rochel, Natacha

    2011-01-01

    Background The 1α,25-dihydroxy-3-epi-vitamin-D3 (1α,25(OH)2-3-epi-D3), a natural metabolite of the seco-steroid vitamin D3, exerts its biological activity through binding to its cognate vitamin D nuclear receptor (VDR), a ligand dependent transcription regulator. In vivo action of 1α,25(OH)2-3-epi-D3 is tissue-specific and exhibits lowest calcemic effect compared to that induced by 1α,25(OH)2D3. To further unveil the structural mechanism and structure-activity relationships of 1α,25(OH)2-3-epi-D3 and its receptor complex, we characterized some of its in vitro biological properties and solved its crystal structure complexed with human VDR ligand-binding domain (LBD). Methodology/Principal Findings In the present study, we report the more effective synthesis with fewer steps that provides higher yield of the 3-epimer of the 1α,25(OH)2D3. We solved the crystal structure of its complex with the human VDR-LBD and found that this natural metabolite displays specific adaptation of the ligand-binding pocket, as the 3-epimer maintains the number of hydrogen bonds by an alternative water-mediated interaction to compensate the abolished interaction with Ser278. In addition, the biological activity of the 1α,25(OH)2-3-epi-D3 in primary human keratinocytes and biochemical properties are comparable to 1α,25(OH)2D3. Conclusions/Significance The physiological role of this pathway as the specific biological action of the 3-epimer remains unclear. However, its high metabolic stability together with its significant biologic activity makes this natural metabolite an interesting ligand for clinical applications. Our new findings contribute to a better understanding at molecular level how natural metabolites of 1α,25(OH)2D3 lead to significant activity in biological systems and we conclude that the C3-epimerization pathway produces an active metabolite with similar biochemical and biological properties to those of the 1α,25(OH)2D3. PMID:21483824

  7. 1α,25(OH2-3-epi-vitamin D3, a natural physiological metabolite of vitamin D3: its synthesis, biological activity and crystal structure with its receptor.

    Ferdinand Molnár

    Full Text Available BACKGROUND: The 1α,25-dihydroxy-3-epi-vitamin-D3 (1α,25(OH2-3-epi-D3, a natural metabolite of the seco-steroid vitamin D3, exerts its biological activity through binding to its cognate vitamin D nuclear receptor (VDR, a ligand dependent transcription regulator. In vivo action of 1α,25(OH2-3-epi-D3 is tissue-specific and exhibits lowest calcemic effect compared to that induced by 1α,25(OH2D3. To further unveil the structural mechanism and structure-activity relationships of 1α,25(OH2-3-epi-D3 and its receptor complex, we characterized some of its in vitro biological properties and solved its crystal structure complexed with human VDR ligand-binding domain (LBD. METHODOLOGY/PRINCIPAL FINDINGS: In the present study, we report the more effective synthesis with fewer steps that provides higher yield of the 3-epimer of the 1α,25(OH2D3. We solved the crystal structure of its complex with the human VDR-LBD and found that this natural metabolite displays specific adaptation of the ligand-binding pocket, as the 3-epimer maintains the number of hydrogen bonds by an alternative water-mediated interaction to compensate the abolished interaction with Ser278. In addition, the biological activity of the 1α,25(OH2-3-epi-D3 in primary human keratinocytes and biochemical properties are comparable to 1α,25(OH2D3. CONCLUSIONS/SIGNIFICANCE: The physiological role of this pathway as the specific biological action of the 3-epimer remains unclear. However, its high metabolic stability together with its significant biologic activity makes this natural metabolite an interesting ligand for clinical applications. Our new findings contribute to a better understanding at molecular level how natural metabolites of 1α,25(OH2D3 lead to significant activity in biological systems and we conclude that the C3-epimerization pathway produces an active metabolite with similar biochemical and biological properties to those of the 1α,25(OH2D3.

  8. Cortical activity in the left and right hemispheres during language-related brain functions

    Lassen, N A; Larsen, B

    1980-01-01

    The blood flow to a given brain region increases as the level of neural activity is augmented. Hence mapping of variations in regional cerebral blood flow affords a means of imaging the activity of various brain regions during various types of brain work. The paper summarizes the patterns of...... auditory feedback (absent in his case) is not a prerequisite for speaking....

  9. ROLE OF ACTIVE ERP PARADIGMS IN AWARENESS DETECTION IN NON RESPONSIVE PATIENTS

    Lugo, Zulay; Lesenfants, Damien; Lehembre, Remy; Laureys, Steven; Noirhomme, Quentin

    2012-01-01

    The role of active vs. passive ERP paradigms in disorders of consciousness is assessed in this case study of a LIS patient. Results show that despite absent P3 in a passive auditory task, the patient displayed significant differences in the active task. This study shows the importance of using a large battery of tests when assessing DOC patients.

  10. A Homozygous [Cys25]PTH(1-84) Mutation That Impairs PTH/PTHrP Receptor Activation Defines a Novel Form of Hypoparathyroidism.

    Lee, Sihoon; Mannstadt, Michael; Guo, Jun; Kim, Seul Min; Yi, Hyon-Seung; Khatri, Ashok; Dean, Thomas; Okazaki, Makoto; Gardella, Thomas J; Jüppner, Harald

    2015-10-01

    Hypocalcemia and hyperphosphatemia are encountered in idiopathic hypoparathyroidism (IHP) and pseudohypoparathyroidism type Ib (PHP1B). In contrast to PHP1B, which is caused by resistance toward parathyroid hormone (PTH), the genetic defects leading to IHP impair production of this important regulator of mineral ion homeostasis. So far, only five PTH mutations were shown to cause IHP, each of which is located in the hormone's pre-pro leader segment and thus impair hormone secretion. In three siblings affected by IHP, we now identified a homozygous arginine-to-cysteine mutation at position 25 (R25C) of the mature PTH(1-84) polypeptide; heterozygous family members are healthy. Depending on the assay used for evaluating these patients, plasma PTH levels were either low or profoundly elevated, thus leading to ambiguities regarding the underlying diagnosis, namely IHP or PHP1B. Consistent with increased PTH levels, recombinant [Cys25]PTH(1-84) and wild-type PTH(1-84) were secreted equally well by transfected COS-7 cells. However, synthetic [Cys25]PTH(1-34) was found to have a lower binding affinity for the PTH receptor type-1 (PTH1R) than PTH(1-34) and consequently a lower efficiency for stimulating cAMP formation in cells expressing this receptor. Consistent with these in vitro findings, long-term infusion of [Cys25]PTH(1-34) resulted only in minimal calcemic and phosphaturic responses, despite readily detectable levels of [Cys25]PTH(1-34) in plasma. The mineral ion abnormalities observed in the three IHP patients are thus most likely caused by the inherited homozygous missense PTH mutation, which reduces bioactivity of the secreted hormone. Based on these findings, screening for PTH(1-84) mutations should be considered when clinical and laboratory findings are consistent with PHP1B, but GNAS methylation changes have been excluded. Differentiating between IHP and PHP1B has considerable implications for genetic counseling, therapy, and long-term outcome because

  11. Thermally active TRPV1 tonically drives central spontaneous glutamate release

    Shoudai, Kiyomitsu; Peters, James H.; McDougall, Stuart J.; Fawley, Jessica A.; Andresen, Michael C.

    2010-01-01

    Central synapses spontaneously release neurotransmitter at low rates. In brainstem, cranial visceral afferent terminals in caudal solitary tract nucleus (NTS) display pronounced activity-dependent asynchronous release of glutamate and this extra release depends on TRPV1 receptors (TRPV1+). Asynchronous release is absent for afferents lacking TRPV1 (TRPV1-) and resting EPSC frequency was greater in TRPV1+. Here, we studied this basal activity difference by assessing thermal sensitivity of spon...

  12. Problemas de saúde responsáveis pelo absenteísmo de trabalhadores de enfermagem de um hospital universitário - DOI: 10.4025/actascihealthsci.v25i2.2232 Health problems responsible for the nursing staff disease-absenteeism in a university hospital - DOI: 10.4025/actascihealthsci.v25i2.2232

    Doris Marli Petry Paulo da Silva

    2003-04-01

    Full Text Available Entende-se por absenteísmo-doença as faltas do trabalhador devidas a problemas de saúde diagnosticados para efeito de licença-saúde. Com o objetivo de identificar os problemas de saúde responsáveis pelo absenteísmo-doença em trabalhadores de enfermagem de um hospital universitário, foi realizado um levantamento retrospectivo das faltas (494 justificadas por licenças-saúde, equivalentes a 1.491 dias de trabalho perdidos em um ano, calculados os índices de absenteísmo-doença e identificadas suas causas. A Unidade de Pediatria apresentou o maior índice de freqüência (If=0,35; a maior porcentagem de tempo perdido (Tp=4,19% ocorreu no Pronto Atendimento, entre auxiliares de Enfermagem, enquanto os enfermeiros apresentaram maiores índices nas UTIs, If=0,17 e Tp=3,93%. Os problemas de saúde foram relacionados aos sistemas respiratório, geniturinário, digestivo e osteomuscular, aos órgãos dos sentidos e ao aparelho reprodutor feminino. Concluímos que os índices de absenteísmo-doença apresentam-se elevados. As causas do adoecimento dos trabalhadores apresentam interface com inadequadas condições de trabalho e fatores de risco presentes no cotidiano da enfermagem.Disease-absenteeism is the frequent absence from work due to some illness. A retrospective survey of the sick-leave (494 absences during a year was performed. Aiming to identify health problems responsible for the nursing staff disease-absenteeism of a university hospital. Both frequency index and percentage of lost time were calculated, and the health problems were identified. The results showed that the highest frequency index occurred in the Pediatric Unit (Fi=0.35 and the highest percentage of lost time due to absence occurred in the Emergency Unit (Lt=4.19% among the Nursing Auxiliaries; the nurses showed the highest frequency in the Intensive Care Unit, Fi=0.17 and Lt=3.93%. Health problems of nursing staff were related to the respiratory and genito

  13. DETERMINATION OF PHYTOCHEMICALS AND ANTIOXIDANT ACTIVITY OF ACORUS CALAMUS RHIZOME

    B L Nanda

    2014-01-01

    Acetone extract of Acorus calamus rhizome was assessed for phytochemical components qualitatively and quantitatively and for its antioxidant activity. The results revealed that the rhizome extract contained alkaloids, carbohydrates, flavonoids, tannins, anthocyanins and phenols. Steroids and saponins were absent in the extract. Phenolics, flavonoids and proanthocyanidins were found to be more in Acorus calamus rhizome extract from quantitative analysis followed by total sugars and tannins res...

  14. The future of vitamin D analogs

    Carlien eLeyssens; Lieve eVerlinden; Annemieke eVerstuyf

    2014-01-01

    The active form of vitamin D3, 1,25-dihydroxyvitamin D3, is a major regulator of bone and calcium homeostasis. In addition, this hormone also inhibits the proliferation and stimulates the differentiation of normal as well as malignant cells. Supraphysiological doses of 1,25-dihydroxyvitamin D3 are required to reduce cancer cell proliferation. However, these doses will lead in vivo to calcemic side effects such as hypercalcemia and hypercalciuria. During the last 25 years, many structural anal...

  15. Evidence that active demethylation mechanisms maintain the genome of carcinoma in situ cells hypomethylated in the adult testis

    Kristensen, D G; Nielsen, J E; Jørgensen, Anne;

    2014-01-01

    absent. Both maintenance and de novo methyltransferases were detected in CIS cells.Conclusion:The data are consistent with the presence of an active DNA de-methylation pathway in CIS cells. The hypomethylated genome of CIS cells may contribute to phenotypic plasticity and invasive capabilities of this...

  16. Why is Dawsonite Absent in CO2 Charged Reservoirs?

    Growth of the sodium-aluminium-hydroxy carbonate dawsonite (NaAl(OH)2CO3) after charging saline aquifers with CO2 has been assumed in a plethora of numerical simulations at different mineralogies, aqueous solutions, pressures and temperatures. It appears however that dawsonite is less abundant than expected in natural CO2 storage analogues if we take into account the thermodynamic stability alone. We have mapped the thermodynamic stability of dawsonite relative to mineral phases like albite, kaolinite and analcime from 37 to 200 C and performed closed-system batch kinetic simulations using a new kinetic expression including a nucleation term based on classical nucleation theory, and a growth term that was based on BCF growth theory. Using this rate equation, we have performed a sensitivity study on dawsonite growth on mineralogy, temperature, CO2 pressure, nucleation rate and its dependencies on temperature and affinity, and on the dawsonite precipitation rate coefficient. Simulations with dawsonite growth disabled showed that the maximum over-saturation reached for dawsonite for seawater-like solutions never exceeded 3-4 times over-saturation. The positive effect on dawsonite growth of increasing the CO2 pressure was mostly neutralized by higher acidity. Decreasing the precipitation rate coefficient by 5 orders of magnitude had a limited effect on the onset of significant growth, but the amount of dawsonite formed at the end of the 1 000 years simulated time was only 37% below the high-rate case. Reducing the nucleation rates had similar effects leading to postponed dawsonite growth. Finally, based on thermodynamic considerations and numerical simulations, we suggest that the potential of dawsonite growth is limited to a medium-temperature window framed by a high thermodynamic stability relative to competing mineral phases at low temperatures, but with rapidly diminishing nucleation and growth rates at lower temperatures constrained by energy barriers. (authors)

  17. Pituitary and ovarian function in women with congenitally absent uterus.

    Ylikorkala, O; Viinikka, L

    1979-01-01

    Pituitary-ovarian function was studied in 2 women with congenital absence of the uterus and the vagina. Daily serum levels of luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin, estradiol-17beta, and progesterone were measured by radioimmunoassay for 1 month. Gonadotropins and ovarian steroids showed normal cyclic variations in both patients. Prolactin was persistently elevated in one of them, who also demonstrated 2 abnormally high testosterone values among 6 determinations. The pituitary gland was stimulated by intravenous leasing hormone (LRH-TRY). Gonadotropin and prolactin responses to LRH-TRH were normal. The lifelong absence of the uterus does not alter the function of the pituitary-ovarian axis. The relation of hyperprolactinemia to this syndrome must be ascertained in a greater number of patients. PMID:104214

  18. Genetics Home Reference: thrombocytopenia-absent radius syndrome

    ... Hemorrhages can damage the brain and lead to intellectual disability. Affected children who survive this period and do not have damaging hemorrhages in the brain usually have a normal life expectancy and normal intellectual development. TAR syndrome is unusual among similar malformations ...

  19. Emphysematous pyelonephritis presenting as a sonologically absent kidney

    Chirag Patel

    2015-01-01

    Full Text Available Emphysematous pyelonephritis is an acute necrotizing infection of the kidney caused by gas-producing organisms. It is being increasingly recognized as an underlying cause of sepsis. We present a case of emphysematous pyelonephritis where the affected kidney was filled with extensive gas. This made the detection of the kidney difficult by ultrasound. Also, this case presents the dilemma of managing a septic patient medically and the risks entailed in surgical intervention in such cases. Most often, these patients have underlying diabetes mellitus with underlying co-morbidities, making indicated surgical management difficult as illustrated by our patient.

  20. Bioaerosol exposure to personnel in a clinical environment absent patients.

    Heimbuch, Brian K; Wallace, William H; Balzli, Charles L; Laning, Michelle L; Harnish, Delbert A; Wander, Joseph D

    2016-01-01

    Nosocomial infections pose a significant and escalating threat to both patients and healthcare workers (HCWs). By their nature, hospitals induce antibiotic resistance in virulent and commensal strains, leading to increasingly severe hospital-acquired infections. This study measured environmental exposure experienced by domestic staff cleaning vacated patient rooms of a community hospital to bacteria in ambient bioaerosols. While they cleaned the room, participants wore an N95 filtering facepiece respirator (FFR), from which coupons were cut and bacteria were extracted, cultured and enumerated. Extrapolation to the full area of the respirator yielded measured exposures of 0.2-1.4 × 10(4) colony-forming units/hour, of which ∼97% collected on the front layer of the N95, suggesting a possible role for minimal respiratory protection in nonpatient environments. Random resistance testing of 1.6% of the isolates showed that ∼70% of both Gram-positive and Gram-negative organisms exhibited resistance to oxacillin and ∼9% of the Gram-positives displayed resistance to vancomycin. These data provide an estimate for mask bioaerosol loading that can be used in risk modeling and to refine strategies for reuse of FFRs during critical shortages. PMID:26375496

  1. Absent posterior tibial artery in a case with clubfoot

    Bursali, Aysegul; Yurdoglu, Cihangir

    2004-01-01

    Absence of the posterior tibial artery is extremely rare. Complete absence of the posterior tibial artery associated with clubfoot was first reported in 1991. As far as we concern, our case is the second. For this reason we built a discussion over our case and related literature. We discuss that a vascular factor may contribute to the etiology of clubfoot and the importance of vascular malformations are emphasized in aspect of surgical dissection.

  2. Nepotism absent in insect societies - or is it?

    Wenseleers, Tom

    2007-01-01

    Inclusive fitness theory, put forward by English biologist William Hamilton in 1964, is considered by many as the most important addition to the theory of natural selection since Darwin. One prediction of the theory is that animals should often show a tendency to nepotistically favour close relatives. Goodisman et al. (2007) test this theory for the first time using molecular methods in a vespine wasp, the eastern yellowjacket, Vespula maculifrons. Somewhat surprisingly, nepotism was found to...

  3. 17. The Absent Writer in The Tragic Muse

    Valtat-Comet, Nelly

    2013-01-01

    The reading I would like to propose here will focus on one particular element of The Tragic Muse that is not foregrounded but implied, and yet informs much of the novel’s development—an aspect that belongs more to the fantastic than the realistic vein in James’s inspiration, by which I mean the overriding absence of a genius of letters and of an ideal text. A large proportion of Book I in The Tragic Muse is devoted to long and rather sophisticated Oxbridge conversations between, mostly, Nick ...

  4. Absent alternatives and insider interests in postcrisis financial reform

    D. Mügge; B. Stellinga

    2010-01-01

    The credit crisis that began in the summer of 2007 has fundamentally challenged much financial regulation and the political institutions that produced it. Measured against the criticisms that have been brought forth against previous financial governance, the extent of governments’ overall reform amb

  5. Integrating Statistics with a Microbiology Laboratory Activity

    William Lorowitz

    2009-12-01

    Full Text Available Statistics is an important tool for microbiologists but is virtually absent from undergraduate laboratory activities. The variables in a stringent protocol, the antibiotic disk diffusion assay described by the National Committee for Clinical Laboratory Standards, were examined by the authors as a means for introducing hypothesis testing and the application of elementary statistical tools. After several experiments, a lab activity was developed where students examine the effect of cell concentration on antibiotic activity and analyze data with the t test. They also collect data independently from the same samples and compare their measurements using analysis of variance (ANOVA. The outcome of the activity, including an assessment tool, indicated that students learned the appropriate use of the t test and ANOVA, gained an appreciation for standardized protocols, and enjoyed the experience.

  6. Factors associated with absenteeism-illness in rural workers in a timber company Factores asociados al absentismo-enfermedad de los trabajadores rurales de una empresa forestal Fatores associados ao absenteísmo-doença dos trabalhadores rurais de uma empresa florestal

    Mariana Roberta Lopes Simões

    2012-08-01

    Full Text Available The monitoring of absenteeism-illness has revealed its high prevalence, and a strong relationship with work. This study aimed to analyze the factors associated with absenteeism-illness among the rural workers in a timber company in Minas Gerais, Brazil. It is an analytical cross-sectional study, carried out among 883 workers. The medical certificates issued in the company over one year were surveyed. For the analysis, use was made of descriptive statistics and bi- and multivariable analyses. The strength of association was measured by the odds ratio (OR with help from logistic regression (pEl acompañamiento del absentismo-enfermedad ha revelado altas prevalencias y fuerte relación con el trabajo. Se objetivó analizar los factores asociados al absentismo-enfermedad de los trabajadores rurales de una empresa forestal en Minas Gerais- Brasil. Se trata de un estudio transversal, analítico, realizado con 883 trabajadores. Fueron levantados los testificados médicos durante un año. Se utilizó para análisis estadístico descriptivo, análisis bi y multivariadas. La fuerza de asociación fue medida por el odds ratio (OR con auxilio de la regresión logística (pO acompanhamento do absenteísmo-doença tem revelado altas prevalências e forte relação com o trabalho. Objetivou-se analisar os fatores associados ao absenteísmo-doença dos trabalhadores rurais de uma empresa florestal em Minas Gerais, Brasil. Trata-se de estudo transversal, analítico, realizado com 883 trabalhadores. Foram levantados os atestados médicos desses trabalhadores, durante um ano. Utilizaram-se, para análise estatística descritiva, análises bi e multivariadas. A força de associação foi medida pelo odds ratio (OR com auxílio da regressão logística (p<0,05. Foi encontrada prevalência de 54,0% de atestados na população. A análise bivariada revelou associação entre a função (ajudante florestal (OR=13,1, marceneiro (OR=15 e operador de motosserra (OR=39

  7. Absenteísmo-doença entre profissionais de enfermagem da rede básica do SUS Campinas Sick leaves among nursing professionals in basic healthcare centers of the Brazilian National Unified Healthcare System - SUS - in the city of Campinas

    Gilson Gehring Junior

    2007-09-01

    Full Text Available O objetivo deste trabalho é analisar índices de absenteísmo-doença entre profissionais de enfermagem de 16 Unidades Básicas de Saúde do Município de Campinas durante o ano de 2002. Foram obtidos junto à seção de Recursos Humanos Descentralizados dados de assiduidade de 311 trabalhadores de enfermagem de Centros de Saúde dos Distrito Norte e Leste do Município de Campinas, e calculados os índices de freqüência, gravidade e proporção de tempo perdido. As variáveis disponibilizadas foram tempo de trabalho na função, categoria funcional, sexo e carga horária semanal. Dados de pesquisas realizadas sobre absenteísmo com pessoal de hospital e do ramo industrial e empresarial foram utilizados para a análise e comparação com o presente estudo. A função de técnico ou auxiliar de enfermagem representou a maioria da população (82,6%, a jornada de trabalho de 36 horas semanais foi realizada por 90,6% dos pesquisados e 93,5% eram do sexo feminino. O índice de freqüência de absenteísmo foi de 2,88 afastamentos iniciados por empregado/ ano, o de gravidade foi de 12,27 dias perdidos por empregado/ano, e a proporção de tempo perdido foi de 5,56% ao ano. Os enfermeiros tiveram índice de freqüência menor que o de auxiliares e técnicos. Os índices encontrados neste trabalho foram maiores do que a maioria dos estudos realizados com profissionais de hospitais. A fim de minimizar o fenômeno como reflexo do impacto das condições desfavoráveis para a saúde do trabalhador, propõe-se diminuir da exposição às cargas de trabalho e melhorar a política de saúde do trabalhador para os servidores da categoria.This report aims to analyze sick leave rates among healthcare professionals from 16 Basic Healthcare Units of the Brazilian National Unified Healthcare System (SUS in the Municipality of Campinas during 2002. Data on worked days and sick leaves of 311 nurses and nursing aides working at Healthcare Centers of the North and

  8. Absenteísmo relacionado à doenças entre membros da equipe de enfermagem de um hospital escola Absentismo debido a las enfermedades entre los miembros del equipo de enfermería en un hospital de enseñanza Disease-related absenteeism among nursing team members in a teaching hospital

    Fernanda Marques da Costa

    2009-02-01

    Full Text Available O artigo apresenta resultados da pesquisa que identificou causas do absenteísmo relacionado a doenças, justificado por atestado ou licença médica, entre os funcionários da enfermagem de um hospital público de Montes Claros - Minas Gerais. Trata-se de uma pesquisa descritivo-exploratória. Os resultados indicam que os principais motivos de afastamento estão relacionados a problemas osteo-musculares e a enfermidades do aparelho respiratório; principalmente entre auxiliares de enfermagem do sexo feminino, casadas, com idade entre 25 e 45 anos, do turno diurno e com mais de um vínculo empregatício. Conclui-se pela necessidade de se desenvolver ações de prevenção e controle do absenteísmo como estratégia para a qualidade de vida dos trabalhadores da enfermagem.El artículo presenta los resultados de la investigación que hay identificado las causas del absentismo debido a enfermedades, las cuales tenían justificativa médica, entre los trabajadores de enfermería de un hospital publico de Montes Claros, MG, Brasil. Tratase de una investigación descriptiva-exploratoria. Los resultados indican que las principales razones del absentismo están relacionados a problemas osteomusculares e a enfermedades respiratorias, principalmente entre los auxiliares de enfermería casadas del sexo femenino y que trabajan en más de un empleo, con edad entre los 25 y 45 años, y que trabajan en el periodo del día. Se concluye que hay una necesidad de desarrollo de acciones de prevención para el control del absentismo como una estrategia para la calidad de vida de los trabajadores de enfermería.The article presents results of the research that identified to causes of disease-related absenteeism, justified by medical license, among nursing employees of a public hospital in Montes Claros, MG, Brazil. This is a descriptive-exploratory study. Results indicate that the main reasons for absenteeism are related to musculoskeletal problems and to respiratory

  9. Using a non-radioisotopic, quantitative TRAP-based me thod detecting telomerase activities in human hepatoma cells

    2000-01-01

    A non-radioisotopic, quantitative TRAP-based telom erase activity assay was established mainly by using SYBR Green-I staining instead of radioisotope. Comparing with conventional radioisotope based method, it was better in reproducibility and accuracy. Using this method, we found telomerase activities were absent in normal human liver cells, while detected in all of four human hepatoma cell lines (BEL-7404, SMMC-7721, QGY-7903 and HCCM) without significant differences.

  10. Median nerve SEP after a high medullary lesion: preserved N18 and absent P14 components (case report PESS do nervo mediano após lesão bulbar alta: componente N18 preservado e P14 ausente. Relato de caso

    G. M. MANZANO

    1999-06-01

    Full Text Available Median nerve SEPs recorded from a patient with a high medullary lesion are described. The lesion involved the anteromedial and anterolateral right upper third of the medulla, as documented by MRI. Forty one days after the lesion, left median nerve SEP showed preserved N18 and absent P14 and N20 components; stimulation of the right median nerve evoked normal responses. These findings agree with the proposition that low medullary levels are involved in the generation of the N18 component of the median nerve SEP.Descrevemos os potenciais evocados somatossensitivos obtidos por estimulação dos nervos medianos em um paciente apresentando uma lesão localizada, envolvendo as porções anteromedial e anterolateral do terço superior do bulbo, documentada por ressonância nuclear magnética. Quarenta e um dias após o estabelecimento da lesão os potenciais evocados por estimulação do nervo mediano esquerdo evidenciaram ausência dos componentes P14 e N20 e preservação do componente N18; após estimulação do nervo mediano direito as respostas apresentaram-se normais. Estes achados estão de acordo com a sugestão de que as porções baixas do bulbo estão envolvidas na geração do componente N18.

  11. Research progresses of heat shock protein 10 and human males absent on the first in diagnosing and treating ednometrial carcinoma of uterus%热休克蛋白10和人组蛋白乙酰化酶在子宫内膜癌诊治中的研究现状

    张娜; 张林燕

    2016-01-01

    子宫内膜癌是严重威胁妇女健康的恶性肿瘤之一.近年来,肿瘤标志物在子宫内膜癌的诊断、病情监测及评估预后等方面的研究不断深入.已有研究表明,人组蛋白乙酰化酶(hMOF)和热休克蛋白10(HSP10)与子宫内膜癌的发生、发展及其生物学行为有着密切的关系.本文就子宫内膜癌中hMOF和HSP10的临床应用进行综述.%Ednometrial carcinoma of uterus (EMC) is one of the malignant tumors threatening women's health seriously.In recent years,the researches of tumor makers in diagnosing EMC,monitoring condition and assessing prognosis have been investigated further.It has been shown that human males absent on the first (hMOF) and (heat shock protein 10,HSP10) are closely correlated with occurrence,development and biological behaviors of EMC.This paper reviewed the clinical applications of hMOF and HSP10 in EMC.

  12. Phosphoproteins and the activation of the neutrophil respiratory burst oxidase

    The respiratory burst oxidase is a neutrophil enzyme that converts oxygen to O2-. It is dormant in resting cells but is activated when the cells are exposed to phorbol myristate acetate (PMA). PMA also induces the incorporation of 32P into certain neutrophil proteins. To determine whether phosphorylation of these proteins is related to oxidase activation, protein phosphorylation was studied in patients with chronic granulomatous disease (GCD), a group of inherited conditions in which oxidase activity is missing. In normals, neutrophil activation by PMA is associated with the phosphorylation inter alia of 48K proteins at pI 7.3 and 7.8. There is also inconstant phosphorylation of a 48K protein at pI 6.8. In 4 patients with X-linked chronic granulomatous disease (CGD), phosphorylation of pp48/6.8 and pp48/7.3 was absent, while in autosomal recessive CGD, phosphorylation of all 3 of these proteins was absent in 3 patients and significantly diminished in a fourth. These results suggest that the phosphorylation of these proteins is related to the activation of the respiratory burst oxidase. By peptide mapping, these 3 proteins appear to consist of a single peptide species whose pI variability may be due to post-translational modification. The only phosphoamino acid found in pp48/7.3 was phosphoserine

  13. Phosphoproteins and the activation of the neutrophil respiratory burst oxidase

    Okamura, N.; Curnutte, J.T.; Babior, B.M.

    1987-05-01

    The respiratory burst oxidase is a neutrophil enzyme that converts oxygen to O/sub 2//sup -/. It is dormant in resting cells but is activated when the cells are exposed to phorbol myristate acetate (PMA). PMA also induces the incorporation of /sup 32/P into certain neutrophil proteins. To determine whether phosphorylation of these proteins is related to oxidase activation, protein phosphorylation was studied in patients with chronic granulomatous disease (GCD), a group of inherited conditions in which oxidase activity is missing. In normals, neutrophil activation by PMA is associated with the phosphorylation inter alia of 48K proteins at pI 7.3 and 7.8. There is also inconstant phosphorylation of a 48K protein at pI 6.8. In 4 patients with X-linked chronic granulomatous disease (CGD), phosphorylation of pp48/6.8 and pp48/7.3 was absent, while in autosomal recessive CGD, phosphorylation of all 3 of these proteins was absent in 3 patients and significantly diminished in a fourth. These results suggest that the phosphorylation of these proteins is related to the activation of the respiratory burst oxidase. By peptide mapping, these 3 proteins appear to consist of a single peptide species whose pI variability may be due to post-translational modification. The only phosphoamino acid found in pp48/7.3 was phosphoserine.

  14. Source distribution of neuromagnetic slow wave activity in schizophrenic and depressive patients

    Wienbruch, Christian; Moratti, Stephan; Elbert, Thomas; Vogel, Ulrike; Fehr, Thorsten; Kissler, Johanna; Schiller, August; Rockstroh, Brigitte

    2003-01-01

    Objective: Focal slow waves in the delta and theta frequency range frequently appear in psychopathological conditions. Due to their focal nature they can be localized by dipole modeling. We previously reported regional clustering of slow waves in temporal and parietal cortex of schizophrenic patients whereas such activity is largely absent in normals. Here we examine, to what extent distribution of slow wave generators differentiates schizophrenic from depressive syndromes. Methods: The regio...

  15. A novel DNA joining activity catalyzed by T4 DNA ligase.

    Western, L M; Rose, S. J.

    1991-01-01

    The use of T4 and E. coli DNA ligases in genetic engineering technology is usually associated with nick-closing activity in double stranded DNA or ligation of 'sticky-ends' to produce recombinant DNA molecules. We describe in this communication the ability of T4 DNA ligase to catalyze intramolecular loop formation between annealed oligodeoxyribonucleotides wherein Watson-Crick base pairing is absent on one side of the ligation site. Enzyme concentration, loop size, substrate specificity, and ...

  16. In vitro synergistic activity between bismuth subcitrate and various antimicrobial agents against Campylobacter pyloridis (C. pylori).

    Van Caekenberghe, D L; Breyssens, J

    1987-01-01

    The in vitro interactions between bismuth subcitrate and a variety of antimicrobial agents against 12 Campylobacter pyloridis (C. pylori) isolates were studied by the agar dilution checkerboard technique. The combination of bismuth subcitrate with the older quinolone, oxolinic acid, produced synergistic activity against all strains. This observation, however, could not be extended to the (aryl) fluoroquinolones, norfloxacin, ofloxacin, and difloxacin, since synergy was rare or absent when bis...

  17. Odor-mediated taste learning requires dorsal hippocampus, but not basolateral amygdala activity

    Wheeler, Daniel S.; Chang, Stephen E.; Holland, Peter C

    2012-01-01

    Mediated learning is a unique cognitive phenomenon in which mental representations of physically absent stimuli enter into associations with directly-activated representations of physically present stimuli. Three experiments investigated the functional physiology of mediated learning involving the use of odor-taste associations. In Experiments 1a and 1b, basolateral amygdala lesions failed to attenuate mediated taste aversion learning. In Experiment 2, dorsal hippocampus inactivation impaired...

  18. Phytochemical analysis of stevia and stachys on contents biological active substances

    B. Sarsenbaev; V. Мursaliyeva; N. Sultanova; L. Мамоnov; B. Ussenbekov

    2012-01-01

    Results of phytochemical analysis of stevia and stachys are showed up. It is revealed that tubers of stachys consist big amount amino acides, carbohydrates, glycosidated triterpenoides, small amount of alkaloids, flavanoides, too little organic acides. Tannins are absent. Acropetal distribution of glycosides was in stevia. Young leaves of stevia and fresh tubers of stachys are perspective raw material for biological active supplements (BAS), special products and food.

  19. Initial Free Recall Data Characterized and Explained By Activation Theory of Short Term Memory

    Tarnow, Dr. Eugen

    2011-01-01

    The initial recall distribution in a free recall experiment is shown to be predictably different from the overall free recall distribution including an offset which can cause the least remembered items to be almost completely absent from the first recall. Using the overall free recall distribution as input and a single parameter describing the probability of simultaneous reactivated items per number of items in the presented list, activation theory not only qualitatively but quantitatively d...

  20. Modelo Matemático para Predição da Acidose Metabólica no Nascimento em Gestações com Diástole Zero ou Reversa Mathematical Model to Predict Metabolic Acidosis at Birth in Pregnancies with Absent or Reversed End-Diastolic Velocity

    Roseli Mieko Yamamoto Nomura

    2002-05-01

    Full Text Available Objetivos: elaborar modelo matemático baseado em parâmetros da cardiotocografia (CTG anteparto de repouso para predição da acidose metabólica no nascimento, caracterizada por valores do excesso de bases (BE inferiores a -10 mEq/L, em gestações com diagnóstico de diástole zero (DZ ou reversa (DR na dopplervelocimetria das artérias umbilicais. Métodos: foi estudada a última cardiotocografia de 127 gestantes de alto risco com diagnóstico de DZ ou DR, pela análise visual do traçado. Os parâmetros da CTG estudados foram a variabilidade da FCF, acelerações transitórias, desacelerações e padrão pseudo-sinusoidal. Entre as características da gravidez foram analisadas a idade gestacional, o intervalo em dias entre o diagnóstico da DZ ou DR e o parto. O modelo de regressão logística foi aplicado na determinação do melhor modelo matemático para predição da acidose. Resultados: a acidose metabólica ocorreu em 51 casos (40,2%. O modelo matemático para predição da acidose contemplou os seguintes parâmetros: intervalo em dias entre o diagnóstico da DZ ou DR e o parto (X1, idade gestacional em semanas (X2, variabilidade da FCF Purpose: to develop a mathematical model based on the fetal heart rate (FHR monitoring to predict metabolic acidosis at birth (base excess < -10 mEq/L, in pregnancies with absent or reversed end-diastolic velocity (AREDV in the umbilical arteries. Methods: the last FHR tracing of 127 AREDV cases was studied by visual analysis. The analyzed parameters included: gestational age, interval between AREDV diagnosis and delivery, FHR variability, FHR accelerations, decelerations, and sinusoidal- like pattern. Multivariate logistic regression was applied to find the best mathematical model to predict acidosis. Results: metabolic acidosis at birth occurred in 51 cases (40.2%. The model included the parameters: interval between AREDV diagnosis and delivery (X1, gestational age in weeks (X2, FHR variability <5

  1. Fatores Prognósticos para o Óbito Perinatal em Gestações com Diástole Zero ou Reversa na Dopplervelocimetria das Artérias Umbilicais Prognostic Parameters for Perinatal Death in Pregnancies with Absent or Reversed End-Diastolic Flow Velocity in the Umbilical Arteries

    Roseli Mieko Yamamoto

    2000-01-01

    Full Text Available Objetivos: estudar os fatores prognósticos para o óbito perinatal em gestações com diagnóstico de diástole zero (DZ ou reversa (DR na dopplervelocimetria das artérias umbilicais. Métodos: foram analisadas retrospectivamente 204 gestantes com DZ ou DR, sendo realizados os exames de cardiotocografia, perfil biofísico fetal, índice do líquido amniótico e dopplervelocimetria do ducto venoso e das artérias umbilicais, uterinas, aorta e cerebral média. Em 170 casos foi aplicado o modelo de regressão logística para determinar a variável com melhor acurácia na predição do óbito perinatal. Resultados: a mortalidade foi de 28 casos de óbito fetal (13,7% e 45 de óbito pós-natal (22,1%. Houve correlação significativa entre os óbitos e as variáveis analisadas. A proporção de óbitos no grupo com recém-nascidos de peso inferior a 1.000 g foi de 74,7% e no grupo com idade gestacional inferior a 31 semanas, de 66,3%. Na regressão logística, o peso do recém-nascido foi a melhor variável capaz de predizer o óbito perinatal (pPurpose: to study the prognostic parameters for perinatal death in pregnancies with absent or reversed end-diastolic flow velocity on umbilical artery dopplervelocimetry. Methods: two hundred and four pregnancies were retrospectively reviewed. The methods used were cardiotocography, fetal biophysical profile, amniotic fluid index and dopplervelocimetry of ductus venosus, fetal aorta, middle cerebral artery, umbilical arteries and uterine artery. The logistic regression model was applied to one hundred and seventy cases in order to determine the most accurate variable for predicting perinatal death. Results: the mortality rates were: 28 cases of intrauterine fetal death (13.7% and 45 neonatal deaths (22.1%. A statistically significant correlation was found between death and the studied variables. The perinatal death rate in the group with birth weight below 1,000 g was 74.7%, and in the group with

  2. Resveratrol inhibits Trypanosoma cruzi arginine kinase and exerts a trypanocidal activity.

    Valera Vera, Edward A; Sayé, Melisa; Reigada, Chantal; Damasceno, Flávia S; Silber, Ariel M; Miranda, Mariana R; Pereira, Claudio A

    2016-06-01

    Arginine kinase catalyzes the reversible transphosphorylation between ADP and phosphoarginine which plays a critical role in the maintenance of cellular energy homeostasis. Arginine kinase from the protozoan parasite Trypanosoma cruzi, the etiologic agent of Chagas disease, meets the requirements to be considered as a potential therapeutic target for rational drug design including being absent in its mammalian hosts. In this study a group of polyphenolic compounds was evaluated as potential inhibitors of arginine kinase using molecular docking techniques. Among the analyzed compounds with the lowest free binding energy to the arginine kinase active site (market price; and (3) has as a well-defined target enzyme which is absent in the mammalian host, it is a promising compound as a trypanocidal drug for Chagas disease. PMID:26976067

  3. Activation of Coagulation by Administration of Recombinant Factor VIIa Elicits Interleukin 6 (IL-6) and IL-8 Release in Healthy Human Subjects

    de Jonge, Evert; Friederich, Philip W.; Vlasuk, George P.; Rote, William E.; Vroom, Margaretha B.; Levi, Marcel; van der Poll, Tom

    2003-01-01

    The activation of coagulation has been shown to contribute to proinflammatory responses in animal and in vitro experiments. Here we report that the activation of coagulation in healthy human subjects by the administration of recombinant factor VIIa also elicits a small but significant increase in the concentrations of interleukin 6 (IL-6) and IL-8 in plasma. This increase was absent when the subjects were pretreated with recombinant nematode anticoagulant protein c2, the inhibitor of tissue factor-factor VIIa. PMID:12738659

  4. Activation of Coagulation by Administration of Recombinant Factor VIIa Elicits Interleukin 6 (IL-6) and IL-8 Release in Healthy Human Subjects

    de Jonge, Evert; Friederich, Philip W.; Vlasuk, George P.; Rote, William E.; Vroom, Margaretha B.; Levi, Marcel; van der Poll, Tom

    2003-01-01

    The activation of coagulation has been shown to contribute to proinflammatory responses in animal and in vitro experiments. Here we report that the activation of coagulation in healthy human subjects by the administration of recombinant factor VIIa also elicits a small but significant increase in the concentrations of interleukin 6 (IL-6) and IL-8 in plasma. This increase was absent when the subjects were pretreated with recombinant nematode anticoagulant protein c2, the inhibitor of tissue f...

  5. Zoo visitors' understanding of terms denoting research activity.

    Carson, Lloyd

    2014-07-01

    Zoos have increasingly sought to justify their existence by reference to a scientific role particularly in the domains of animal welfare and conservation. Given recent initiatives by the UK government to foster public engagement with science, it is timely to investigate public attitudes towards primary research activity by zoos. This study reports the views of 83 visitors to Edinburgh Zoo. Within certain items in a structured interview noun terms denoting research activity were manipulated ("research" versus "studies") as was their qualification (adjective "scientific" present or absent before the noun term). "Research" was associated with a restricted and negative perception of investigatory activity. This effect was intensified when the noun term was preceded by "scientific". It is concluded that there is a continuing need to challenge public perceptions, particularly of the phrase "scientific research"; that in the meantime zoos should perhaps exercise caution when using it in relation to their activities. PMID:25414921

  6. Cardiotocografia em gestações com diástole zero ou reversa nas artérias umbilicais: análise dos resultados perinatais Cardiotocography in pregnancies with absent or reversed end-diastolic velocity in the umbilical arteries: analisys of perinatal outcome

    Roseli Mieko Yamamoto Nomura

    2003-01-01

    Full Text Available OBJETIVOS: Estudar, em gestações com diástole zero (DZ ou reversa (DR, dopplervelocimetria das artérias umbilicais os resultados perinatais de acordo com os parâmetros cardiotocográficos. MÉTODOS: Entre 1993 e 2000, foram selecionadas 127 gestantes com DZ ou DR. A classificação adotada para a cardiotocografia foi ativo (normal, hipoativo (suspeito e inativo (alterado Os parâmetros analisados foram: variabilidade da FCF, acelerações transitórias, DIP II, DIP umbilical, desaceleração prolongada e padrão pseudo sinusoidal. RESULTADOS: Houve associação (pOBJECTIVES: To study the correlation between cardiotocography parameters and perinatal outcome in pregnancies with absent or reversed end-diastolic velocity (AERDV in the umbilical arteries. METHODS: One hundred and twenty-seven cases presenting with AREDV followed between 1993 and 2000 were selected for analysis. The last cardiotocographic tracing performed on the day of delivery was reviewed and the following parameters were considered: magnitude of long-term variability, presence or absence of acceleration, late deceleration, variable deceleration, prolonged deceleration, pseudo sinusoidal pattern and the classification in normal, suspected and abnormal tracing. RESULTS: A significant (p<0.05 association was found between abnormal tracing and acidemia at birth (pH inferior to 7.20 with 71.9%, first minute Apgar score inferior to seven (73.4%, newborn intubation at delivery (64.1%, and early neonatal death (20.3%. The absence of acceleration was associated (p<0.05 to acidemia at birth (58.8%, first minute Apgar score inferior to seven (67.7%, newborn intubation at delivery (51% and neonatal death (29.4%. Low FHR variability (<5bpm was associated to (p<0.05: acidemia at birth (88.5%, newborn intubation at delivery (69.2%, early neonatal death (34.6% and neonatal death (42.3%. Late decelerations were significantly (p<0,05 related to acidemia at birth.(78.2%. The severe variable

  7. 基于SIWAREX FTA称重技术的烟箱缺条检测及剔除系统设计%Design of Carton Box Absent Packets Detecting and Sorting out System Based on the Weighing Technology of SIWAREX FTA

    张华军

    2012-01-01

    为防止因卷烟包装过程中装封箱机烟箱缺条质量事故给烟草企业造成不良影响,通过分析烟箱缺条的原因,比较现有的烟箱缺条检测方法,设计了一种基于物联网平台的分牌号的集成化称重式烟箱缺条检测及剔除系统.采用条形码技术、西门子S7 300 PLC和SIWAREX FTA称重模块等技术,改进了传统称重式系统的漏检、误检等缺陷,烟箱缺条检出率提高至98%,误检率则降低至5‰以内.该系统可有效减少烟箱缺条事故发生,并对产品质量实现了追踪、追溯,也为烟箱缺条质量事故认定提供了真实、准确的证据.%To solve the problem of quality accident of shortage of carton box at filling and sealing machines in cigarette making and packing workshop,trace the process and product quality,a carton box absent packets detecting and sorting out system with weighing technology of SIWAREX FTA is designed in the paper. After analyzing the reason and compare the solution,Barcode,Siemens S7 300 PLC,SIWAREX FTA and weighing modules are used in the system to build an integrated weighing system which implements the quick measure on line to improve the traditional weigh solution with the disadvantage of leak and mistake detecting. This system can effectively reduce the accident of shortage of carton box and offer the true evidence for the cogni-zance of the accident. The quality of the product can also be tracked and traced.

  8. Physical activity

    Physical activity -- which includes an active lifestyle and routine exercise -- plus eating well, is the best way to ... shortness of breath when you are active Build physical activity into your regular routine Simple lifestyle changes can ...

  9. Coincident helminth infection modulates systemic inflammation and immune activation in active pulmonary tuberculosis.

    Parakkal Jovvian George

    Full Text Available Helminth infections are known to modulate innate and adaptive immune responses in active and latent tuberculosis (TB. However, the role of helminth infections in modulating responses associated with inflammation and immune activation (reflecting disease activity and/or severity in TB is not known.We measured markers of inflammation and immune activation in active pulmonary TB individuals (ATB with co-incidental Strongyloides stercoralis (Ss infection. These included systemic levels of acute phase proteins, matrix metalloproteinases and their endogenous inhibitors and immune activation markers. As a control, we measured the systemic levels of the same molecules in TB-uninfected individuals (NTB with or without Ss infection.Our data confirm that ATB is associated with elevated levels of the various measured molecules when compared to those seen in NTB. Our data also reveal that co-incident Ss infection in ATB individuals is associated with significantly decreased circulating levels of acute phase proteins, matrix metalloproteinases, tissue inhibitors of matrix metalloproteinases as well as the systemic immune activation markers, sCD14 and sCD163. These changes are specific to ATB since they are absent in NTB individuals with Ss infection.Our data therefore reveal a profound effect of Ss infection on the markers associated with TB disease activity and severity and indicate that co-incidental helminth infections might dampen the severity of TB disease.

  10. Antioxidant and Antiproliferative Activities of the Essential Oils from Thymbra capitata and Thymus Species Grown in Portugal

    Maria Graça Miguel; Custódia Gago; Maria Dulce Antunes; Cristina Megías; Isabel Cortés-Giraldo; Javier Vioque; A. Sofia Lima; A. Cristina Figueiredo

    2015-01-01

    Copyright © 2015 Maria Graça Miguel et al. The antioxidant and antiproliferative activities of the essential oils from Thymbra capitata and Thymus species grown in Portugal were evaluated. Thymbra and Thymus essential oils were grouped into two clusters: Cluster I in which carvacrol, thymol, p-cymene, α-terpineol, and γ-terpinene dominated and Cluster II in which thymol and carvacrol were absent and the main constituent was linalool. The ability for scavenging ABTS·+ and peroxyl free radicals...

  11. Absenteísmo-doença, modelo demanda-controle e suporte social: um estudo caso-controle aninhado em uma coorte de trabalhadores de hospitais, Santa Catarina, Brasil Sickness-absenteeism, job demand-control model, and social support: a case-control study nested in a cohort of hospital workers, Santa Catarina, Brazil

    Kleber dos Santos

    2011-12-01

    Full Text Available O objetivo do estudo foi identificar a contribuição do Modelo Demanda-Controle (MDC e do suporte social para duração do absenteísmo-doença segundo Licenças para Tratamento de Saúde (LTS nos trabalhadores de hospitais (servidores públicos da Secretaria de Estado da Saúde de Santa Catarina (SES/SC, Brasil. Entre as secretarias deste estado, a SES/SC tem a segunda maior frequência de LTS, com destaque nos hospitais. Este é um estudo caso-controle aninhado em uma coorte de trabalhadores de hospitais da SES/SC, com acompanhamento entre 01/07/2008 e 30/06/2009. Os casos foram aqueles que apresentaram LTS nesse período, os critérios de pareamento foram sexo, faixa etária e setor de trabalho. O desfecho foi o total de dias de LTS, as variáveis de exposição foram MDC e o suporte social do supervisor e do colega. Utilizou-se dados primários e secundários, nas análises a regressão Zero-inflado Binomial Negativa, e as variáveis socioeconômicas e ocupacionais nos ajustes. O banco de dados para as análises teve 425 (144 casos; 281 controles participantes. O baixo suporte do colega aumentou a expectativa de dias de LTS em 2,04 (IC95%: 1,05-3,93, unido ao trabalho de baixa exigência aumentou 2,68 (IC95%: 1,37-5,27 e ao de alta exigência (iso-strain 78% (IC95%: 1,02-3,12 mais do que o MDC com alto suporte do colega. Este estudo mostrou a importância do suporte do colega sobre a duração absenteísmo-doença, e auxiliou conjeturar que a variável tempo nos trabalhadores de hospital leva a adaptação às exigências das tarefas e a monotonia. Intervenções no relacionamento entre os trabalhadores provavelmente diminuirão a duração de LTS.The aim of the study was to identify the Job Demand-Control (JDC and social support contribution for sickness-absenteeism duration of Health Treatment Licences (HTL in hospital workers (civil servants of the Santa Catarina State Department of Health of (SES/SC, Brazil. These health workers have

  12. Inflammasome activation in decompensated liver cirrhosis

    2016-01-01

    Inflammation participates in the pathogenesis of many liver diseases, including liver cirrhosis. Certaininflammatory citokines, such as interleukin (IL)-1β andIL-18, are produced after the activation of a multiproteincomplex known as the inflammasome. Activation of theinflammasome has been documented in several liverdiseases, but its role in the development and progressionof liver cirrhosis or the complications associated withthis disease is still largely unknown. We have recentlystudied the impact of the inflammasome in the sterileinflammatory response that takes place in the asciticfluid of patients with decompensated cirrhosis, providingevidence that activation of the absent in melanoma2 (AIM2) inflammasome is an important response inthese patients. Ascitic fluid-derived macrophages wereable to mount a very robust AIM2-mediated responseeven in the absence of a priming signal, which is usuallyrequired for the full activation of all the inflammasomes.In addition, high level of inflammasome activation inthese patients was associated with a higher degree ofliver disease and an increased incidence of spontaneousbacterial peritonitis. These results may help explain theexacerbated inflammatory response that usually occursin patients with decompensated cirrhosis in the absenceof detectable infections. Thus, inflammasomes shouldbe considered as possible therapeutic targets in sterileinflammatory complications in patients with cirrhosis.

  13. Presença ausente e ausência presente do Estado na produção do espaço para o turismo no Vale do Ribeira paulista Présence absente et absence présente de l’État dans la production de l’espace touristique dans la vallée du Ribeira, São Paulo

    Carolina Todesco

    2010-07-01

    desenvolvimento do ecoturismo na região, em certa medida, dependa do nível de organização social de cada município ou comunidade e de suas capacidades em formular projetos para a captação de recursos públicos, ou do interesse de organizações exógenas em implantar projetos na região. Período, portanto, em que temos no Vale do Ribeira uma “ausência presente do Estado”.La région de la vallée du Ribeira, situé dans le sud de l’État de São Paulo, possède 20% des vestiges de la Forêt Atlantique du Brésil et a le plus bas indices de développement humain de l’État de plus grande densité technico-scientifique-informationnel et de capital du pays. L’actuelle configuration socio-spatiale de cette région est le produit d’un processus historique marqué par l’action et l’inaction de l’État, qui joue son rôle dans la production de l’espace de différentes manières, en fonction du mouvement de l’histoire. Lors de l’examen des politiques publiques pour le développement régional concernant la vallée, des années 60 aux début des années 80, on observe une action de l’Etat centralisée et inefficace, sans participation de la société civile dans le processus de production de ses politiques, une période que nous qualifions de «présence absente de l’État dans la vallée du Ribeira“. Depuis les années 80, un certain nombre de facteurs ont conduit la région à des problèmes et des défis nouveaux. La politique de démocratisation, l’avancée du néolibéralisme, la préoccupation croissante au sujet des questions d’environnement, le nombre croissant d’ONGs et de la décentralisation des politiques publiques amènent l’État à une nouvelle façon de jouer son rôle dans la production de l’espace. Dans ce contexte, il y a un net changement d’orientation des actions ciblées de l’État à la vallée du Ribeira, principalement influencé par les ONG environnementales, qui fonctionnent maintenant activement dans l

  14. Absenteísmo - doença na equipe de enfermagem: relação com a taxa de ocupação Ausentismo - enfermedad en el equipo de enfermería: relación con la tasa de ocupación Absenteeism - disease in the nursing staff: relationship with the occupation tax

    Tania Regina Sancinetti

    2009-12-01

    ón, sugeriendo que los profesionales se ausentaron por enfermedad después de haberen sido sometidos a ritmos mayores de trabajoThis is a qualitative descriptive, transversal study aiming to analyze the amount and causes of sick leave of nursing professionals and its relationship with the occupation tax of the hospitalization units in a teaching hospital. The methodology was divided into two phases: demographic characterization of professionals and identification and analysis of absences regarding the amount and type of sick leaves, medical diagnosis and its relationship with the occupation tax of the Hospital. The nursing professionals presented the greatest amount of sick leaves. Diseases of the osteomuscular system and of the connective tissue represented 4,957 days (41.5% of absences and mental and behavioral disorders 3,393 days (28.4%. The monthly percentage of sick licenses was inversely proportional to the occupation tax, suggesting that professionals were absent due to diseases after being submitted to greater work load.

  15. PLASMINOGEN ACTIVATOR OF YERSINIA PESTIS

    V. V. Evseeva

    2015-01-01

    Full Text Available Plague has been the cause of three pandemics and has led to the death of millions of people. Plague is a typical zoonosis caused by Yersinia pestis that circulates in populations of wild rodents inhabiting natural plague foci on all continents except for Australia. Transmission of plague is provided by flea bites. Circulation of Y. pestis in natural plague foci is supported by a numerous of pathogenicity factors. This review explores one of them, plasminogen activator Pla. This protein is one of representatives of omptins, a family of enterobacterial outer membrane proteases that are responsible for colonization of specific organs or even infection generalization as a result of successful overcoming of the host innate immunity. The review reflects the history of its discovery and studying of its genetic control, biosynthesis, isolation and purification, physicochemical properties. Highly purified preparations of plasminogen activator are deficient in enzymatic activities but renaturation in the presence of Y. pestis lipooligosaccharide restores enzymatic properties of Pla. This pathogenicity factor is absent in representatives of the most ancient phylogenetic group of the plague pathogen, bv. caucasica, while the ancestor of other groups of Y. pestis subsp. microtus obtained in result of horizontal transfer Pla isoform with characteristics similar to properties of omptins from the less virulent enterobacteria. After that in the course of microevolution the “classic” isoform of Pla with increased protease activity was selected that is typical of all highly virulent for humans strains of Y. pestis subsp. pestis. The “classic” isoform of Pla Y. pestis is functionally similar to mammalian plasminogen activators transforming plasminogen into plasmin with the help of limited proteolysis. Pla protease activating plasminogen and also degrading the main plasmin inhibitor — α2-antiplasmin and, respectively, determining Y. pestis ability to lyse

  16. Physical activity

    ... this page: //medlineplus.gov/ency/article/001941.htm Physical activity To use the sharing features on this page, please enable JavaScript. Physical activity -- which includes an active lifestyle and routine exercise -- ...

  17. An fMRI study of neuronal activation in schizophrenia patients with and without previous cannabis use

    Else-Marie eLøberg

    2012-10-01

    Full Text Available Previous studies have mostly shown positive effects of cannabis use on cognition in patients with schizophrenia, which could reflect lower neurocognitive vulnerability. There are however no studies comparing whether such cognitive differences have neuronal correlates. Thus, the aim of the present study was to compare whether patients with previous cannabis use differ in brain activation from patients who has never used cannabis. The patients groups were compared on the ability to up-regulate an effort mode network during a cognitive task and down-regulate activation in the same network during a task-absent condition. Task-present and task-absent brain activation was measured by functional magnetic resonance neuroimaging (fMRI. Twenty-six patients with a DSM-IV and ICD-10 diagnosis of schizophrenia were grouped into a previous cannabis user group and a no-cannabis group. An auditory dichotic listening task with instructions of attention focus on either the right or left ear stimulus was used to tap verbal processing, attention and cognitive control, calculated as an aggregate score. When comparing the two groups, there were remaining activations in the task-present condition for the cannabis group, not seen in the no-cannabis group, while there was remaining activation in the task-absent condition for the no-cannabis group, not seen in the cannabis group. Thus, the patients with previous cannabis use showed increased activation in an effort mode network and decreased activation in the default mode network as compared to the no-cannabis group. It is concluded that the present study show some differences in brain activation to a cognitively challenging task between previous cannabis and no-cannabis schizophrenia patients.

  18. A presença ausente do racial: discursos políticos e pedagógicos sobre História, "Portugal" e (pós-colonialismo The absent presence of racial: political and pedagogical discourses on History, 'Portugal' and (postcolonialism

    Marta Araújo

    2013-03-01

    in/visibility. The racial, being considered as a result from the presence of the "other" from the colony, is therefore naturally irrelevant to the formation of the national-metropolitan space and, more generally, to the notion of "Europeanness". From this analysis, we illustrate the depoliticization of racism through the naturalization of colonialism and the reification of the 'immigrant imaginary'; accordingly, racism is understood as something that happens to "others" - ethno-racially marked - but that does not relate to what "we" are, a "we" that, actually, is never questioned. This paper is divided in three sections: (i we examine the consolidation of the absent presence of the 'racial' considering how a cycle of silence and consensus has been consolidated by the very initiatives to combat racism - marked by anti-racialism - by global organizations such as UNESCO. We further examine how the dissolution of the 'racial' is being produced by the association between racism and immigration in the contemporary European context, both in politics and in faculty; (ii we present our empirical work and analysis of contemporary history textbooks in Portugal. We show that debates about history and, more specifically, about the teaching of colonialism and slavery have emptied its political relevance and evaded the 'racial' in the understanding of so-called Atlantic slavery, since the fifteenth to the nineteenth centuries, (iii finally, we conclude with an analysis of the contours of what we call the "(post colonial consensus" in Portugal, within a broader context of the emptying of the plausibility of a vocabulary that takes into account 'racial'/racism in contemporary societies.

  19. Long head of the triceps muscle transfer for active elbow flexion in arthrogryposis.

    Gogola, Gloria R; Ezaki, Marybeth; Oishi, Scott N; Gharbaoui, Idris; Bennett, James B

    2010-06-01

    Arthrogryposis is a condition characterized by symmetric, nonprogressive joint contractures and weak or absent musculature that is present at birth. The amyoplasia form is the most common, and in this group, the elbow is frequently involved, typically in an extension contracture bilaterally. Active elbow flexion is weak or absent, but active extension is spared. This elbow dysfunction poses a significant disability for affected children. Sensation and cognitive development is normal in children with arthrogryposis, and as a group they demonstrate a remarkable degree of adaptability to their deformities. The goal of any treatment is to facilitate the child's functional independence. This article describes the surgical technique of transfer of the long head of the triceps into the proximal ulna to provide active elbow flexion in children with arthrogryposis. The goal of the procedure is to reliably achieve antigravity active flexion while preserving active extension. It has the advantages of technical simplicity and minimal donor site morbidity. By adding this procedure to the existing options for treating this challenging condition, a surgeon is better able to tailor intervention to an individual child's strength and available donor muscles. PMID:20526167

  20. The regulation of l-asparaginase activity in rats and mice. Effects of normal and malignant growth, of sex and of dietary changes

    Bonetti, E.; Abbondanza, Ada; Corte, E. Della; Stirpe, F.

    1969-01-01

    1. The activity of l-asparaginase was very low in the liver of newborn rats and mice, and increased within a few days of birth. 2. In rats, but not in mice, the enzyme activity was higher in females than in males, was enhanced by administration of oestradiol, and was decreased by gonadectomy. 3. The enzyme activity decreased in mice starved or fed on a low-protein diet; in rats it was enhanced by starvation, by feeding them on a high-protein diet, or by administration of l-asparagine. 4. The asparaginase activity was decreased in regenerating liver, and was almost absent in the Morris hepatoma 5123. PMID:4311065

  1. Compound heterozygosity with a novel S222N GALT mutation leads to atypical galactosemia with loss of GALT activity in erythrocytes but little evidence of clinical disease

    Benjamin Cocanougher; Umut Aypar; Amber McDonald; Linda Hasadsri; Bennett, Michael J; Edward Highsmith, W.; Kristin D'Aco

    2015-01-01

    Galactosemia is an inborn error of galactose metabolism caused by mutations in the GALT gene. Though early detection and galactose restriction prevent severe liver disease, affected individuals have persistently elevated biomarkers and often neuro-developmental symptoms. We present a teenage compound heterozygote for a known pathogenic mutation (H132Q) and a novel variant of unknown significance (S222N), with nearly absent erythrocyte GALT enzyme activity but normal biomarkers and only mild a...

  2. Features for voice activity detection: a comparative analysis

    Graf, Simon; Herbig, Tobias; Buck, Markus; Schmidt, Gerhard

    2015-12-01

    In many speech signal processing applications, voice activity detection (VAD) plays an essential role for separating an audio stream into time intervals that contain speech activity and time intervals where speech is absent. Many features that reflect the presence of speech were introduced in literature. However, to our knowledge, no extensive comparison has been provided yet. In this article, we therefore present a structured overview of several established VAD features that target at different properties of speech. We categorize the features with respect to properties that are exploited, such as power, harmonicity, or modulation, and evaluate the performance of some dedicated features. The importance of temporal context is discussed in relation to latency restrictions imposed by different applications. Our analyses allow for selecting promising VAD features and finding a reasonable trade-off between performance and complexity.

  3. Triclosan antagonizes fluconazole activity against Candida albicans.

    Higgins, J

    2012-01-01

    Triclosan is a broad-spectrum antimicrobial compound commonly used in oral hygiene products. Investigation of its activity against Candida albicans showed that triclosan was fungicidal at concentrations of 16 mg\\/L. However, at subinhibitory concentrations (0.5-2 mg\\/L), triclosan antagonized the activity of fluconazole. Although triclosan induced CDR1 expression in C. albicans, antagonism was still observed in cdr1Δ and cdr2Δ strains. Triclosan did not affect fluconazole uptake or alter total membrane sterol content, but did induce the expression of FAS1 and FAS2, indicating that its mode of action may involve inhibition of fatty acid synthesis, as it does in prokaryotes. However, FAS2 mutants did not exhibit increased susceptibility to triclosan, and overexpression of both FAS1 and FAS2 alleles did not alter triclosan susceptibility. Unexpectedly, the antagonistic effect was specific for C. albicans under hypha-inducing conditions and was absent in the non-filamentous efg1Δ strain. This antagonism may be due to the membranotropic activity of triclosan and the unique composition of hyphal membranes.

  4. Get Active

    ... feeling the benefits of getting active, such as sleeping better or getting toned. Here are 2 ways to add more activity to your life. Be active for longer each time. If you are walking 3 days a week for 30 minutes, try ...

  5. A decaying factor accounts for contained activity in neuronal networks with no need of hierarchical or modular organization

    Amancio, Diego R; Costa, Luciano da F

    2012-01-01

    The mechanisms responsible for contention of activity in systems represented by networks are crucial in various phenomena, as in diseases such as epilepsy that affects the neuronal networks, and for information dissemination in social networks. The first models to account for contained activity included triggering and inhibition processes, but they cannot be applied to social networks where inhibition is clearly absent. A recent model showed that contained activity can be achieved with no need of inhibition processes provided that the network is subdivided in modules (communities). In this paper, we introduce a new concept inspired in the Hebbian theory through which activity contention is reached by incorporating a dynamics based on a decaying activity in a random walk mechanism preferential to the node activity. Upon selecting the decay coefficient within a proper range, we observed sustained activity in all the networks tested, viz. random, Barabasi-Albert and geographical networks. The generality of this ...

  6. DCP1 forms asymmetric trimers to assemble into active mRNA decapping complexes in metazoa

    Tritschler, Felix; Braun, Joerg E.; Motz, Carina; Igreja, Catia; Haas, Gabrielle; Truffault, Vincent; Izaurralde, Elisa; Weichenrieder, Oliver

    2009-01-01

    DCP1 stimulates the decapping enzyme DCP2, which removes the mRNA 5′ cap structure committing mRNAs to degradation. In multicellular eukaryotes, DCP1-DCP2 interaction is stabilized by additional proteins, including EDC4. However, most information on DCP2 activation stems from studies in S. cerevisiae, which lacks EDC4. Furthermore, DCP1 orthologs from multicellular eukaryotes have a C-terminal extension, absent in fungi. Here, we show that in metazoa, a conserved DCP1 C-terminal domain drives...

  7. Structure-activity relationships of constrained phenylethylamine ligands for the serotonin 5-ht2 receptors

    Isberg, Vignir; Paine, James; Leth-Petersen, Sebastian;

    2013-01-01

    class. Conformationally constrained phenethylamine analogs have demonstrated that for optimal activity the free lone pair electrons of the 2-oxygen must be oriented syn and the 5-oxygen lone pairs anti relative to the ethylamine moiety. Also the ethyl linker has been constrained providing information...... showed that the 1,2-heterocyclized compounds can be accommodated in the binding site. Conformational analysis showed that 11 can only bind in a higher-energy conformation, which would explain its absent or low affinity. The amine and 2-oxygen interactions with D3.32 and S3.36, respectively, can form but...

  8. Two major medicinal honeys have different mechanisms of bactericidal activity.

    Paulus H S Kwakman

    Full Text Available Honey is increasingly valued for its antibacterial activity, but knowledge regarding the mechanism of action is still incomplete. We assessed the bactericidal activity and mechanism of action of Revamil® source (RS honey and manuka honey, the sources of two major medical-grade honeys. RS honey killed Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa within 2 hours, whereas manuka honey had such rapid activity only against B. subtilis. After 24 hours of incubation, both honeys killed all tested bacteria, including methicillin-resistant Staphylococcus aureus, but manuka honey retained activity up to higher dilutions than RS honey. Bee defensin-1 and H₂O₂ were the major factors involved in rapid bactericidal activity of RS honey. These factors were absent in manuka honey, but this honey contained 44-fold higher concentrations of methylglyoxal than RS honey. Methylglyoxal was a major bactericidal factor in manuka honey, but after neutralization of this compound manuka honey retained bactericidal activity due to several unknown factors. RS and manuka honey have highly distinct compositions of bactericidal factors, resulting in large differences in bactericidal activity.

  9. Activation analysis

    The neutron activation analysis, which appears to be in limits for further advance, is the most suitable for providing information on the principal as well as the microcomponents in any sample of solid form. Then, instrumental activation analysis is capable of determination of far many elements in various samples. Principally on the neutron activation analysis, the following are described in literature survey from 1982 to middle 1984: bibliography, review, data collection, etc.; problems in spectral analysis and measurement; activation analysis with neutrons; charged particle and photo-nucleus reactions; chemical separation, isotopic dilution activation analysis; molecular activation analysis; standard materials; life and its relation samples; environmental, food, court trial and archaeological samples; space and earth sciences. (Mori, K.)

  10. Enhanced removal of detergent and recovery of enzymatic activity following sodium dodecyl sulfate-polyacrylamide gel electrophoresis: UUse of casein in gel wash buffer

    The inclusion of 1% casein or bovine serum albumin in buffer used to reactivate enzymes subjected to sodium dodecyl sulfate (SDS)-polyacrylamide electrophoresis resulted in accelerated removal of SDS and restoration of nuclease and beta-galactosidase enzyme activities. Nuclease and beta-galactosidase activities which are absent from gels after longer wash procedures are detectable with this technique. Enzyme activity in gels prepared with SDS which contained inhibitory contaminants was partially restored by the casein wash procedure. The threshold of detection of two-dimensionally separated deoxyribonuclease I using the casein wash procedure was 1 picogram

  11. Why is Dawsonite Absent in CO2 Charged Reservoirs? Pourquoi la dawsonite est-elle absente des réservoirs chargés en CO2 ?

    Hellevang H.

    2011-03-01

    Full Text Available Growth of the sodiumaluminium-hydroxy carbonate dawsonite (NaAl(OH2CO3 after charging saline aquifers with CO2 has been assumed in a plethora of numerical simulations at different mineralogies, aqueous solutions, pressures and temperatures. It appears however that dawsonite is less abundant than expected in natural CO2 storage analogues if we take into account the thermodynamic stability alone. We have mapped the thermodynamic stability of dawsonite relative to mineral phases like albite, kaolinite and analcime from 37° to 200°C and performed closed-system batch kinetic simulations using a new kinetic expression including a nucleation term based on classical nucleation theory, and a growth term that was based on BCF growth theory. Using this rate equation, we have performed a sensitivity study on dawsonite growth on mineralogy, temperature, CO2 pressure, nucleation rate and its dependencies on temperature and affinity, and on the dawsonite precipitation rate coefficient. Simulations with dawsonite growth disabled showed that the maximum oversaturation reached for dawsonite for seawater-like solutions never exceeded 3-4 times oversaturation. The positive effect on dawsonite growth of increasing the CO2 pressure was mostly neutralized by higher acidity. Decreasing the precipitation rate coefficient by 5 orders of magnitude had a limited effect on the onset of significant growth, but the amount of dawsonite formed at the end of the 1 000 years simulated time was only 37% below the high-rate case. Reducing the nucleation rates had similar effects leading to postponed dawsonite growth. Finally, based on thermodynamic considerations and numerical simulations, we suggest that the potential of dawsonite growth is limited to a medium-temperature window framed by a high thermodynamic stability relative to competing mineral phases at low temperatures, but with rapidly diminishing nucleation and growth rates at lower temperatures constrained by energy barriers. La possibilité pour la dawsonite – un hydroxy-carbonate de sodium et d’aluminium (NaAl(OH2CO3 – de précipiter dans les aquifères salins dès lors que l’on y injecte du CO2 a été suggérée dans maintes simulations menées sur différentes compositions (minéraux et solution aqueuse et dans des conditions de pression et de température variées. Pourtant, sur le strict plan de la stabilité thermodynamique, la dawsonite paraît moins communément répandue que l’on pourrait s’y attendre dans les analogues naturels de stockages de CO2. On a cartographié la stabilité thermodynamique de la dawsonite par rapport à des phases minérales comme l’albite, la kaolinite et l’analcime, entre 37 et 200 °C, puis on a simulé numériquement des évolutions minérales en système fermé à l’aide d’un nouveau formalisme cinétique pour la précipitation, formalisme qui inclut (1 un terme de nucléation, basé sur la théorie classique de la nucléation, et (2 un terme de croissance, dérivant de la théorie BCF de la croissance. Utilisant cette équation de vitesse, on a réalisé une étude de sensibilité pour examiner comment la croissance de la dawsonite varie avec la composition minérale, la température, la pression partielle de CO2, la vitesse de nucléation – et les variations de cette dernière avec la température et l’affinité chimique –, et enfin la constante de vitesse adoptée pour la loi de précipitation de la dawsonite. Lorsqu’on empêche la dawsonite de précipiter, le rapport de sur-saturation ne dépasse jamais 3 ou 4 pour des solutions de type eau de mer. La propension accrue à précipiter si la pression partielle de CO2 augmente est contrebalancée par l’effet d’acidification de la solution. Diminuer jusqu’à 5 ordres de grandeur la vitesse de précipitation n’a qu’un effet limité sur le démarrage d’une croissance significative, et au bout de 1 000 ans de simulation la quantité de dawsonite formée est de 37 % ce qu’elle est dans le régime de précipitation maximale. Réduire le taux de nucléation a le même effet de retarder la croissance du minéral. Au bout du compte, sur la base de considérations thermodynamiques et au vu des simulations présentées, on suggère que le potentiel de croissance de la dawsonite est restreint à une fenêtre de températures médianes. À basse température, la stabilité relativement grande de la dawsonite vis-à-vis des minéraux en compétition est contrecarrée par la forte diminution des vitesses de nucléation et de croissance, ce qui reflète une puissante barrière énergétique.

  12. Active ratchets

    Angelani, L.; Costanzo, A.; Di Leonardo, R.

    2011-12-01

    We analyze self-propelling organisms, or active particles, in a periodic asymmetric potential. Unlike standard ratchet effect for Brownian particles requiring external forcing, in the case of active particles asymmetric potential alone produces a net drift speed (active ratchet effect). By using theoretical models and numerical simulations we demonstrate the emergence of the rectification process in the presence of an asymmetric piecewise periodic potential. The broken spatial symmetry (external potential) and time symmetry (active particles) are sufficient ingredients to sustain unidirectional transport. Our findings open the way to new mechanisms to move in directional manner motile organisms by using external periodic static fields.

  13. Physical Activity

    Andersen, Lars Bo; Anderssen, Sigmund Alfred; Wisløff, Ulrik;

    2014-01-01

    Andersen LB, Anderssen SA, Wisløff U, Hellénius M-L, Fogelholm M, Ekelund U. (Expert Group) Nordic Nutrition Recommendations 2012. Integrating nutrition and physical activity. Chapter: Physical Activity p. 195-217.Nordic Counsil of Ministers.......Andersen LB, Anderssen SA, Wisløff U, Hellénius M-L, Fogelholm M, Ekelund U. (Expert Group) Nordic Nutrition Recommendations 2012. Integrating nutrition and physical activity. Chapter: Physical Activity p. 195-217.Nordic Counsil of Ministers....

  14. Lack of on-going adaptations in the soleus muscle activity during walking in patients affected by large-fiber neuropathy

    Nazarena, Mazzaro; Grey, Michael James; Sinkjær, Thomas;

    2005-01-01

    The aim of this study was to investigate the contribution of feedback from large-diameter sensory fibers to the adaptation of soleus muscle activity after small ankle trajectory modifications during human walking. Small-amplitude and slow-velocity ankle dorsiflexion enhancements and reductions were...... applied during the stance phase of the gait cycle to mimic the normal variability of the ankle trajectory during walking. Patients with demyelination of large sensory fibers (Charcot-Marie-Tooth type 1A and antibodies to myelin-associated glycoprotein neuropathy) and age-matched controls participated in...... this study. The patients had absent light-touch sense in the toes and feet and absent quadriceps and Achilles tendon reflexes, indicating functional loss of large sensory fibers. Moreover, their soleus stretch reflex response consisted of a single electromyographic (EMG) burst with delayed onset and...

  15. The Receptor for Advanced Glycation End Products Activates the AIM2 Inflammasome in Acute Pancreatitis.

    Kang, Rui; Chen, Ruochan; Xie, Min; Cao, Lizhi; Lotze, Michael T; Tang, Daolin; Zeh, Herbert J

    2016-05-15

    Severe acute pancreatitis (AP) is responsible for significant human morbidity and mortality worldwide. Currently, no specific treatments for AP exist, primarily due to the lack of a mechanistic understanding of sterile inflammation and the resultant multisystem organ dysfunction, the pathologic response of AP linked to early death. In this study, we demonstrate that the class III major histocompatibility region III receptor for advanced glycation end products (RAGE) contributes to AP by modulating inflammasome activation in macrophages. RAGE mediated nucleosome-induced absent in melanoma 2 (but not NLRP3) inflammasome activation by modulating dsRNA-dependent protein kinase phosphorylation in macrophages. Pharmacological and genetic inhibition of the RAGE-dsRNA-dependent protein kinase pathway attenuated the release of inflammasome-dependent exosomal leaderless cytokines (e.g., IL-1β and high-mobility group box 1) in vitro. RAGE or absent in melanoma 2 depletion in mice limited tissue injury, reduced systemic inflammation, and protected against AP induced by l-arginine or cerulein in experimental animal models. These findings define a novel role for RAGE in the propagation of the innate immune response with activation of the nucleosome-mediated inflammasome and will help guide future development of therapeutic strategies to treat AP. PMID:27045109

  16. Activity report

    The Department of Physics and Measurement Technology, Biology and Chemistry (IFM) presents every year a progress report containing a brief description of activities in research and education within the department. The report is intended as an information for colleagues and institutions. The present report contains activities for the academic year July 1989 to June 1990

  17. Indoor Activities

    ... so you can do some lifting while you watch TV. Walk around the house when you talk on the phone. Make an extra trip up and down the stairs when you do the laundry. Download the Tip Sheet Indoor Activities (PDF, 739.53 KB) You Might Also Like Sun Safety Have Fun. Be Active with Your Dog! ...

  18. [Relation between parameters of efferent activity and limb position during fictive scratching in the decerebrate cat].

    Shimanskiĭ, Iu P; Baev, K V

    1986-01-01

    The influence of tonic afferent inflow conditioned by ipsilateral hindlimb position on the spinal generator of scratching efferent activity parameters was investigated in immobilized decerebrated cats. When the afferent inflow was absent (after the hindlimb deafferentation) strong correlation between the motor activity parameters of successive "scratch" cycles was observed. In case of the intact afferentation this correlation was somewhat weaker and decreased with shifting the hindlimb from the aimed position to overaimed or deflecting backward. The discovered statistically significant correlations between the parameters of different motor activity phases and their dependence on the hindlimb position are organized in such a way that they can provide stability of the aimed position of the hindlimb carrying out oscillatory movements. According to the parameters of the efferent activity and their correlation type, the hindlimb deafferentation is most similar to the aimed position of the hindlimb. PMID:3774066

  19. PRELIMINARY PHYTOCHEMICAL INVESTIGATION AND ANTHELMINTIC ACTIVITY OF ACACIA SUMA (ROXB BARKS

    Acharyya Suman

    2011-01-01

    Full Text Available The present work was conducted to investigate the preliminary phytochemical studies and anthelmintic activities on the bark of Acacia suma (Roxb. Family- Fabaceae against adult Indian earthworms, Pheretima posthuma. Various concentrations (5-25 mg/ml of each extract along with the reference samples (Piperazine citrate, Albendazole were subjected for anthelmintic activity study. The qualitative test revealed that the petroleum ether extracts contained only terpenoids but chloroform and hydroalcoholic (Methanol 70% v/v extracts exhibited the presence of carbohydrates, alkaloids, glycosides, flavonoids, tannins and saponins but amino acids and steroids were absent. All the extracts showed anthelmintic activity when compared with petroleum ether and chloroform extracts. The anthelmintic activity of hydroalcoholic extract was comparable with reference drugs.

  20. Emergent smectic order in simple active particle models

    Romanczuk, Pawel; Chaté, Hugues; Chen, Leiming; Ngo, Sandrine; Toner, John

    2016-06-01

    Novel ‘smectic-P’ behavior, in which self-propelled particles form rows and move on average along them, occurs generically within the orientationally ordered phase of simple models that we simulate. Both apolar (head–tail symmetric) and polar (head–tail asymmetric) models with aligning and repulsive interactions exhibit slow algebraic decay of smectic order with system size up to some finite length scale, after which faster decay occurs. In the apolar case, this scale is that of an undulation instability of the rows. In the polar case, this instability is absent, but traveling fluctuations disrupt the rows in large systems and motion and smectic order may spontaneously globally rotate. These observations agree with a new hydrodynamic theory which we present here. Variants of our models also exhibit active smectic ‘A’ and ‘C’ order, with motion orthogonal and oblique to the layers respectively.

  1. Activated Charcoal

    ... reduce intestinal gas (flatulence), lower cholesterol levels, prevent hangover, and treat bile flow problems (cholestasis) during pregnancy. ... pregnancy, according to some early research reports. Preventing hangover. Activated charcoal is included in some hangover remedies, ...

  2. Active Galaxies

    Kilerci Eser, Ece

    Galaxy formation and evolution is one of the main research themes of modern astronomy. Active galaxies such as Active Galactic Nuclei (AGN) and Ultraluminous Infrared Galaxies (ULIRGs) are important evolutionary stages of galaxies. The ULIRG stage is mostly associated with galaxy mergers and...... interactions. During the interactions of gas-rich galaxies, the gas inflows towards the centers of the galaxies and can trigger both star formation and AGN activity. The ULIRG stage includes rapid star formation activity and fast black hole growth that is enshrouded by dust. Once the AGN emission is...... one is related to the mass estimates of supermassive black holes (SMBHs). Mass estimates of SMBHs are important to understand the formation and evolution of SMBHs and their host galaxies. Black hole masses in Type 1 AGN are measured with the reverberation mapping (RM) technique. Reverberation mapping...

  3. Instilling activism in trustees.

    Stern, A R

    1980-01-01

    One of the great challenges in America today is the stewardship of its not-for-profit institutions. While the profit motive may be absent in the running of such organizations, the demand for thoughtful budgeting, cost accounting, and financial skills still exists. Voluntary community hospitals in particular--given the economic realities of the day--need leadership, reciprocal education, and future planning. Speaking from his own experience, the author presents an activist's perspective on hospital boards; but service to other types of not-for-profit institutions involves similar demands for change. PMID:10245408

  4. Relevance of rhodopsin studies for GPCR activation.

    Deupi, Xavier

    2014-05-01

    Rhodopsin, the dim-light photoreceptor present in the rod cells of the retina, is both a retinal-binding protein and a G protein-coupled receptor (GPCR). Due to this conjunction, it benefits from an arsenal of spectroscopy techniques that can be used for its characterization, while being a model system for the important family of Class A (also referred to as "rhodopsin-like") GPCRs. For instance, rhodopsin has been a crucial player in the field of GPCR structural biology. Until 2007, it was the only GPCR for which a high-resolution crystal structure was available, so all structure-activity analyses on GPCRs, from structure-based drug discovery to studies of structural changes upon activation, were based on rhodopsin. At present, about a third of currently available GPCR structures are still from rhodopsin. In this review, I show some examples of how these structures can still be used to gain insight into general aspects of GPCR activation. First, the analysis of the third intracellular loop in rhodopsin structures allows us to gain an understanding of the structural and dynamic properties of this region, which is absent (due to protein engineering or poor electron density) in most of the currently available GPCR structures. Second, a detailed analysis of the structure of the transmembrane domains in inactive, intermediate and active rhodopsin structures allows us to detect early conformational changes in the process of ligand-induced GPCR activation. Finally, the analysis of a conserved ligand-activated transmission switch in the transmembrane bundle of GPCRs in the context of the rhodopsin activation cycle, allows us to suggest that the structures of many of the currently available agonist-bound GPCRs may correspond to intermediate active states. While the focus in GPCR structural biology is inevitably moving away from rhodopsin, in other aspects rhodopsin is still at the forefront. For instance, the first studies of the structural basis of disease mutants in

  5. Active colloids

    Aranson, Igor S.

    2013-01-01

    A colloidal suspension is a heterogeneous fluid containing solid microscopic particles. Colloids play an important role in our everyday life, from food and pharmaceutical industries to medicine and nanotechnology. It is useful to distinguish two major classes of colloidal suspensions: equilibrium and active, i.e., maintained out of thermodynamic equilibrium by external electric or magnetic fields, light, chemical reactions, or hydrodynamic shear flow. While the properties of equilibrium colloidal suspensions are fairly well understood, active colloids pose a formidable challenge, and the research is in its early exploratory stage. One of the most remarkable properties of active colloids is the possibility of dynamic self-assembly, a natural tendency of simple building blocks to organize into complex functional architectures. Examples range from tunable, self-healing colloidal crystals and membranes to self-assembled microswimmers and robots. Active colloidal suspensions may exhibit material properties not present in their equilibrium counterparts, e.g., reduced viscosity and enhanced self-diffusivity, etc. This study surveys the most recent developments in the physics of active colloids, both in synthetic and living systems, with the aim of elucidation of the fundamental physical mechanisms governing self-assembly and collective behavior.

  6. Physics activities

    As we move into the 21st Century, nuclear technology is on the verge of rejuvenation in advanced Member States and of expansion in developing Member States. The principal responsibilities of the IAEA are transferring technologies, co-ordinating scientific research, managing specialized projects and maintaining analytical quality control. The IAEA physics activities provide assistance with nuclear instrumentation, promote more effective utilization of research reactors and accelerators, and facilitate global co-operation in nuclear fusion research. These activities will help Member States improve their standards of living through the benefits of nuclear technology. This booklet presents a brief profile on the physics activities and involvement in these fields of the Physics Section, IAEA

  7. Regulatory activities

    This publication, compiled in 8 chapters, presents the regulatory system developed by the Nuclear Regulatory Authority (NRA) of the Argentine Republic. The following activities and developed topics in this document describe: the evolution of the nuclear regulatory activity in Argentina; the Argentine regulatory system; the nuclear regulatory laws and standards; the inspection and safeguards of nuclear facilities; the emergency systems; the environmental systems; the environmental monitoring; the analysis laboratories on physical and biological dosimetry, prenatal irradiation, internal irradiation, radiation measurements, detection techniques on nuclear testing, medical program on radiation protection; the institutional relations with national and international organization; the training courses and meeting; the technical information

  8. DNA methylation in porcine preimplantation embryos developed in vivo or produced by in vitro fertilization, parthenogenetic activation and somatic cell nuclear transfer

    Deshmukh, Rahul Shahaji; Østrup, Olga; Østrup, Esben;

    2011-01-01

    vitro fertilized (IVF), somatic cell nuclear transfer (SCNT) and parthenogenetically activated (PA) embryos were evaluated for DNA methylation quantification at different developmental stages. Fertilized (IV and IVF) one-cell stages lacked a substantial active demethylation of the paternal genome....... Embryos produced under in vitro conditions had higher levels of DNA methylation than IV. A lineage-specific DNA methylation (hypermethylation of the inner cell mass and hypomethylation of the trophectoderm) was observed in porcine IV late blastocysts, but was absent in PA- and SCNT-derived blastocysts...

  9. DNA methylation in porcine preimplantation embryos developed in-vivo or produced by in-vitro fertilization, parthenogenetic activation and somatic cell nuclear transfer

    Deshmukh, Rahul Shahaji; Østrup, Olga; Østrup, Esben;

    2011-01-01

    vitro fertilized (IVF), somatic cell nuclear transfer (SCNT) and parthenogenetically activated (PA) embryos were evaluated for DNA methylation quantification at different developmental stages. Fertilized (IV and IVF) one-cell stages lacked a substantial active demethylation of the paternal genome....... Embryos produced under in vitro conditions had higher levels of DNA methylation than IV. A lineage-specific DNA methylation (hypermethylation of the inner cell mass and hypomethylation of the trophectoderm) was observed in porcine IV late blastocysts, but was absent in PA- and SCNT-derived blastocysts...

  10. Hydrogen Peroxide Stimulates the Ca2+-activated Big-Conductance K Channels (BK) Through cGMP Signaling Pathway in Cultured Human Endothelial Cells

    Dong, De-Li; Yue, Peng; Yang, Bao-Feng; Wang, Wen-Hui

    2008-01-01

    We used the whole cell patch-clamp technique to examine the effect of hydrogen peroxide (H2O2) on the Ca2+-activated BK channels in human endothelial cells. We confirmed the previous finding that a 200 pS BK channel activity was detected when the cell membrane potential was clamped at 50 mV. Application of H2O2 or adding glucose oxidase (GO) stimulated BK channels. The stimulatory effect of H2O2 and GO was absent in cells treated with ebselen, a scavenger of reactive oxygen species (ROS). To ...

  11. Dynamic changes in cytosolic ATP levels in cultured glutamatergic neurons during NMDA-induced synaptic activity supported by glucose or lactate

    Lange, Sofie Cecilie; Winkler, Ulrike; Andresen, Lars;

    2015-01-01

    biosensor Ateam1.03YEMK. While inducing synaptic activity by subjecting cultured neurons to two 30 s pulses of NMDA (30 µM) with a 4 min interval, changes in relative ATP levels were measured in the presence of lactate (1 mM), glucose (2.5 mM) or the combination of the two. ATP levels reversibly declined...... following NMDA-induced neurotransmission activity, as indicated by a reversible 10-20 % decrease in the response of the biosensor. The responses were absent when the NMDA receptor antagonist memantine was present. In the presence of lactate alone, the ATP response dropped significantly more than in the...

  12. Clusters of activated microglia in normal-appearing white matter show signs of innate immune activation

    van Horssen Jack

    2012-07-01

    Full Text Available Abstract Background In brain tissues from multiple sclerosis (MS patients, clusters of activated HLA-DR-expressing microglia, also referred to as preactive lesions, are located throughout the normal-appearing white matter. The aim of this study was to gain more insight into the frequency, distribution and cellular architecture of preactive lesions using a large cohort of well-characterized MS brain samples. Methods Here, we document the frequency of preactive lesions and their association with distinct white matter lesions in a cohort of 21 MS patients. Immunohistochemistry was used to gain further insight into the cellular and molecular composition of preactive lesions. Results Preactive lesions were observed in a majority of MS patients (67% irrespective of disease duration, gender or subtype of disease. Microglial clusters were predominantly observed in the vicinity of active demyelinating lesions and are not associated with T cell infiltrates, axonal alterations, activated astrocytes or blood–brain barrier disruption. Microglia in preactive lesions consistently express interleukin-10 and TNF-α, but not interleukin-4, whereas matrix metalloproteases-2 and −9 are virtually absent in microglial nodules. Interestingly, key subunits of the free-radical-generating enzyme NADPH oxidase-2 were abundantly expressed in microglial clusters. Conclusions The high frequency of preactive lesions suggests that it is unlikely that most of them will progress into full-blown demyelinating lesions. Preactive lesions are not associated with blood–brain barrier disruption, suggesting that an intrinsic trigger of innate immune activation, rather than extrinsic factors crossing a damaged blood–brain barrier, induces the formation of clusters of activated microglia.

  13. P-glycoprotein ATPase activity requires lipids to activate a switch at the first transmission interface.

    Loo, Tip W; Clarke, David M

    2016-04-01

    P-glycoprotein (P-gp) is an ABC (ATP-Binding Cassette) drug pump. A common feature of ABC proteins is that they are organized into two wings. Each wing contains a transmembrane domain (TMD) and a nucleotide-binding domain (NBD). Drug substrates and ATP bind at the interface between the TMDs and NBDs, respectively. Drug transport involves ATP-dependent conformational changes between inward- (open, NBDs far apart) and outward-facing (closed, NBDs close together) conformations. P-gps crystallized in the presence of detergent show an open structure. Human P-gp is inactive in detergent but basal ATPase activity is restored upon addition of lipids. The lipids might cause closure of the wings to bring the NBDs close together to allow ATP hydrolysis. We show however, that cross-linking the wings together did not activate ATPase activity when lipids were absent suggesting that lipids may induce other structural changes required for ATPase activity. We then tested the effect of lipids on disulfide cross-linking of mutants at the first transmission interface between intracellular loop 4 (TMD2) and NBD1. Mutants L443C/S909C and L443C/R905C but not G471C/S909C and V472C/S909C were cross-linked with oxidant when in membranes. The mutants were then purified and cross-linked with or without lipids. Mutants G471C/S909C and V472C/S909C cross-linked only in the absence of lipids whereas mutants L443C/S909C and L443C/R905C were cross-linked only in the presence of lipids. The results suggest that lipids activate a switch at the first transmission interface and that the structure of P-gp is different in detergents and lipids. PMID:26944019

  14. A RabGAP protein and BEACH Family proteins regulate contractile vacuole formation and activity and chemotaxis in Dictyostelium

    Du, Fei

    2007-01-01

    The contractile vacuole (CV) system is the osmoregulatory organelle of free-living amoebae and protozoa. I present data showing that the RabGAP RabGAP1 acts as a switch for discharging the CVs into the extracellular medium in Dictyostelium. rabgap1 null (rabgap1⁻) cells have highly enlarged CVs whose structure and activity are aberrant. In rabgap1- cells, the dynamic fusion of the CV with the plasma membrane is absent and the discharge of CV content is inefficient. RabGAP1 localizes to the CV...

  15. Activated Sludge.

    Saunders, F. Michael

    1978-01-01

    Presents the 1978 literature review of wastewater treatment. This review covers: (1) activated sludge process; (2) process control; (3) oxygen uptake and transfer; (4) phosphorus removal; (5) nitrification; (6) industrial wastewater; and (7) aerobic digestion. A list of 136 references is also presented. (HM)

  16. Active house

    Eriksen, Kurt Emil; Olesen, Gitte Gylling Hammershøj

    2010-01-01

    Formålet med dette abstrakt er at illustrere, at huse kan være konstrueret til at basere sig udelukkende på vedvarende energikilder og samtidig være CO2-neutrale og producere mere energi end de forbruger. Active House Visionen undersøger disse muligheder i otte demonstration huse i fem forskellige...

  17. Activation of the Canonical Wnt Signaling Pathway Induces Cementum Regeneration.

    Han, Pingping; Ivanovski, Saso; Crawford, Ross; Xiao, Yin

    2015-07-01

    Canonical Wnt signaling is important in tooth development but it is unclear whether it can induce cementogenesis and promote the regeneration of periodontal tissues lost because of disease. Therefore, the aim of this study is to investigate the influence of canonical Wnt signaling enhancers on human periodontal ligament cell (hPDLCs) cementogenic differentiation in vitro and cementum repair in a rat periodontal defect model. Canonical Wnt signaling was induced by (1) local injection of lithium chloride; (2) local injection of sclerostin antibody; and (3) local injection of a lentiviral construct overexpressing β-catenin. The results showed that the local activation of canonical Wnt signaling resulted in significant new cellular cementum deposition and the formation of well-organized periodontal ligament fibers, which was absent in the control group. In vitro experiments using hPDLCs showed that the Wnt signaling pathway activators significantly increased mineralization, alkaline phosphatase (ALP) activity, and gene and protein expression of the bone and cementum markers osteocalcin (OCN), osteopontin (OPN), cementum protein 1 (CEMP1), and cementum attachment protein (CAP). Our results show that the activation of the canonical Wnt signaling pathway can induce in vivo cementum regeneration and in vitro cementogenic differentiation of hPDLCs. PMID:25556853

  18. Digital Activism

    Skovgaard Jørgensen, Emma; Debiteh Dimma Nielsen, Cecilie

    2015-01-01

    This paper examines the phenomenon of digital activism an how it challenges political systems in transition to democracy. As theoretical framework we will use concepts within sociology and political science as analytical tools to our case studies of China and Egypt, in order to find out which role the different concepts play in each case and how these interrelate with one and other. The project was analyzed from a structuralist interpretive approach, viewing social reality as a practice ...

  19. Activity report

    Yu, S W

    2008-08-11

    This report is aimed to show the author's activities to support the LDRD. The title is 'Investigation of the Double-C Behavior in the Pu-Ga Time-Temperature-Transformation Diagram' The sections are: (1) Sample Holder Test; (2) Calculation of x-ray diffraction patterns; (3) Literature search and preparing publications; (4) Tasks Required for APS Experiments; and (5) Communications.

  20. Hepatoprotective Activity.

    2016-01-01

    The liver performs a vital role in metabolism, secretion, storage, and detoxification of endogenous and exogenous substances. Oxidative stress and free radicals enhance the severity of hepatic damage, which can be overcome by the antioxidant mechanism. Plant extracts can be the best source of such antioxidants and mediate hepatoprotective activity. In this chapter, high-dose paracetamol-induced hepatotoxicity in rat model is discussed with explanations of biochemical and histopathological studies. PMID:26939279

  1. MD-2 determinants of nickel and cobalt-mediated activation of human TLR4.

    Alja Oblak

    Full Text Available Recent findings unexpectedly revealed that human TLR4 can be directly activated by nickel ions. This activation is due to the coordination of nickel by a cluster of histidine residues on the ectodomain of human TLR4, which is absent in most other species. We aimed to elucidate the role of MD-2 in the molecular mechanism of TLR4/MD-2 activation by nickel, as nickel binding site on TLR4 is remote from MD-2, which directly binds the endotoxin as the main pathological activator of TLR4. We identified MD-2 and TLR4 mutants which abolished TLR4/MD-2 receptor activation by endotoxin but could nevertheless be significantly activated by nickel, which acts in synergy with LPS. Human TLR4/MD-2 was also activated by cobalt ions, while copper and cadmium were toxic in the tested concentration range. Activation of TLR4 by cobalt required MD-2 and was abolished by human TLR4 mutations of histidine residues at positions 456 and 458. We demonstrated that activation of TLR4 by nickel and cobalt ions can trigger both the MyD88-dependent and the -independent pathway. Based on our results we propose that predominantly hydrophobic interactions between MD-2 and TLR4 contribute to the stabilization of the TLR4/MD-2/metal ion complex in a conformation that enables activation.

  2. Dietary supplementation with high doses of regular vitamin D3 safely reduces diabetes incidence in NOD mice when given early and long term.

    Takiishi, Tatiana; Ding, Lei; Baeke, Femke; Spagnuolo, Isabella; Sebastiani, Guido; Laureys, Jos; Verstuyf, Annemieke; Carmeliet, Geert; Dotta, Francesco; Van Belle, Tom L; Gysemans, Conny A; Mathieu, Chantal

    2014-06-01

    High doses of the active form of vitamin D3, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], prevent diabetes in the NOD mouse but also elicit unwanted calcemic side effects. Because immune cells themselves can convert vitamin D3 into 1,25(OH)2D3 locally, we hypothesized that dietary vitamin D3 can also prevent disease. Thus, we evaluated whether dietary administration of high doses of regular vitamin D3 (800 IU/day) during different periods of life (pregnancy and lactation, early life [3-14 weeks of age], or lifelong [3-35 weeks of age]) safely prevents diabetes in NOD mice. We found that only lifelong treatment raised serum 25-hydroxyvitamin D3 from 173 nmol/L in controls to 290 nmol/L, without inducing signs of calcemic or bone toxicity, and significantly reduced diabetes development in both male and female NOD mice. This diabetes protection by vitamin D3 correlated with preserved pancreatic insulin content and improved insulitis scores. Moreover, vitamin D3 treatment decreased interferon-γ-positive CD8(+) T cells and increased CD4(+)(CD25(+))FoxP3(+) T cells in pancreatic draining lymph nodes. In conclusion, this study shows for the first time that high doses of regular dietary vitamin D3 can safely prevent diabetes in NOD mice when administered lifelong, although caution is warranted with regards to administering equivalently high doses in humans. PMID:24550187

  3. Interposition of the gallbladder - or the absent common hepatic duct and cystic duct

    Interposition of the gallbladder is a rare anomaly, but its diagnosis is important since it represents a surgically correctable cause of jaundice. The patients present with jaundice, abdominal pain and sometimes an enlarged gallbladder. Radiological diagnosis may be difficult since the condition may be mistaken for a choledochal cyst, hydrops of the gallbladder or Caroli's disease. The ultrasound, cholangiogram and surgical findings of dilated intrahepatic ducts adjacent to a normal or enlarged gallbaldder with no dilatation of the common bile duct are presented in two children with this condition. (orig.)

  4. Interposition of the gallbladder - or the absent common hepatic duct and cystic duct

    Stringer, D.A.; Daneman, A.; Dobranowski, J.; Ein, S.H.; Filler, R.M.; Roberts, E.A.

    1987-02-01

    Interposition of the gallbladder is a rare anomaly, but its diagnosis is important since it represents a surgically correctable cause of jaundice. The patients present with jaundice, abdominal pain and sometimes an enlarged gallbladder. Radiological diagnosis may be difficult since the condition may be mistaken for a choledochal cyst, hydrops of the gallbladder or Caroli's disease. The ultrasound, cholangiogram and surgical findings of dilated intrahepatic ducts adjacent to a normal or enlarged gallbaldder with no dilatation of the common bile duct are presented in two children with this condition.

  5. Absent bystanders and cognitive dissonance: a comment on Timmins & de Vries.

    Paley, John

    2015-04-01

    Timmins & de Vries are more sympathetic to my editorial than other critics, but they take issue with the details. They doubt whether the bystander phenomenon applies to Mid Staffs nurses; they believe that cognitive dissonance is a better explanation of why nurses fail to behave compassionately; and they think that I am 'perhaps a bit rash' to conclude that 'teaching compassion may be fruitless'. In this comment, I discuss all three points. I suggest that the bystander phenomenon is irrelevant; that Timmins & de Vries give an incomplete account of cognitive dissonance; and that it isn't rash to propose that educating nurses 'for compassion' is a red herring. Additionally, I comment on the idea that I wish to mount a 'defence of healthcare staff'. PMID:25549986

  6. Acute audiovestibular deficit with complete ocular tilt reaction and absent VEMPs.

    Goto, Fumiyuki; Ban, Yumiko; Tsutumi, Tomoko

    2011-07-01

    A patient presenting with vertical diplopia along with ocular tilt reaction (OTR) due to peripheral vestibular dysfunction is a rare occurrence. OTR is an eye-head postural reaction consisting of head tilt, skew deviation, conjugated eye cyclotorsion, and alteration of vertical perception, and is thought to occur with central lesions, mainly brainstem lesions. Here, we report a case of a patient who was suffering from left acute peripheral cochleovestibular loss. He had profound deafness and absence of caloric response on the left side. No central lesion was observed on magnetic resonance images. Neuro-ophthalmological examination showed OTR consisting of head tilt, skew deviation with left hypotropia, excyclotorsion, and tilt of the static visual vertical directed to the left side. Both utricular and saccular dysfunctions were identified by the absence of cervical vestibular evoked myogenic potential (cVEMP) and ocular VEMP (oVEMP) on the left side. Within a month, the OTR disappeared completely probably because of vestibular compensation. This is the first report to state that the peripheral otolith dysfunction causing reversible vertical diplopia was identified by objective examinations (VEMP). PMID:21431953

  7. On eigenvalues and eigenfunctions absent in the actual solid state theory

    In this letter new, closed and compact analytic expressions for the evaluation of resonant energies, resonant bound-states, eigenvalues and eigenfunctions for both scattering and bounded n-cell systems are reported. It is shown that for (scattering and bounded) 1-D systems the eigenfunctions Ψμ,ν(z) are simple and well defined functions of the Chebyshev polynomials of the second kind Un, and the energy eigenvalues Eμ,ν (in the μ-th band) are determined by the zeros of these polynomials. New insights on the energy gap and the localization effect induced by phase coherence are shown. (author)

  8. The Earth Is Flat when Personally Significant Experiences with the Sphericity of the Earth Are Absent

    Carbon, Claus-Christian

    2010-01-01

    Participants with personal and without personal experiences with the Earth as a sphere estimated large-scale distances between six cities located on different continents. Cognitive distances were submitted to a specific multidimensional scaling algorithm in the 3D Euclidean space with the constraint that all cities had to lie on the same sphere. A…

  9. Will You Miss Me When I Am Gone? The Economic Consequences of Absent Parents

    Marianne E. Page; Ann Huff Stevens

    2002-01-01

    This paper examines the effects of family structure on the economic resources available to children, using family fixed-effects to control for unobservable characteristics of the family. The effects of divorce on the income and consumption of children born to two-parent households, and the effects of marriage on children born into single-parent households are both considered. In the long-run (six or more years after the most recent divorce) family income falls by 40 to 45% after divorce, and ...

  10. Daddy's Gone to Colorado: Male-Staffed Child Care for Father-Absent Boys.

    Brody, Steve

    1978-01-01

    The article presents the goals, methods, and case examples of The Nutury, a predominantly male-staffed child care center serving single-parent children. The primary goal is to provide consistent relationships with men for children without a male model in their home. Clinical observations reveal positive life-styles and attitudes. (LPG)

  11. Overlying surficial deposits and absent areas for Minnekahta Limestone in the Black Hills area, South Dakota

    U.S. Geological Survey, Department of the Interior — This data set describes areas where the Minnekahta Limestone is directly overlain by surficial deposits, as well as those areas where the Minnekahta Limestone is...

  12. The denial of death: A three-decade long case of absent grief

    Susan Solomon

    2014-01-01

    Full Text Available Bereavement reactions are associated with numerous physical and mental complications. Atypical bereavement reactions have been described but their place in the classificatory system has not been established. We present the case of an eighty-year-old woman who came with the belief that her deceased son was alive. We discuss the diagnostic dilemma she posed and conclude that it may be difficult to differentiate atypical bereavement from other psychiatric illnesses.

  13. Experiences of young adult women with emotionally absent fathers / Emené Peyper

    Peyper, Emené

    2013-01-01

    For many years the focus of research on child well-being and development has been primarily on the dynamics of the mother-child relationship. The mother was seen as the more influential parent as she spends more time with the children. The father’s role in the development process was thus undermined. Fortunately research on the father’s involvement in a child’s life has improved over the past thirty years. Where previously fathers were mostly perceived as the breadwinners and p...

  14. Music and Dissociation : Experiences Without Valence? 'Observing' Self and 'Absent' Self

    Herbert, Ruth

    2013-01-01

    Empirical studies of music listening in everyday life frequently frame individuals' experience of music primarily in terms of emotion and mood. Yet emotions - at least as represented by categorical and dimensional models of emotion - do not account for the totality of subjective experience. This is particularly apparent in the case of a range of so-called 'alternate' or 'altered' states of consciousness including 'flow', aesthetic and spiritual expe-riences. Some researchers have responded by...

  15. IMAGINING THE ABSENT PARTNER - INTIMACY AND IMAGINATION IN LONG-DISTANCE RELATIONSHIPS

    Iveta Jurkane-Hobein

    2015-01-01

    Full Text Available The dominant discourse on relationships in modernity argues for the importance of intimacy, including the intimacy of bodies, for the relationship to be continuous. This raises the question as to how couples that cannot meet face-to-face on a regular basis due to geographical distance maintain intimacy during repetitious non-co-presence. In this article, intimacy is seen as a relational quality that is created and maintained by individuals themselves through practices of intimacy (Jamieson, 2011. The study aims to analyse practices of intimacy in long-distance relationships (LDRs that enable long-distance couples to make their relationship continuous beyond face-to-face encounters. The study is based on 19 in-depth interviews with indi Shrani viduals in Latvia with LDR experience, and argues that the intimacy practices in LDRs trigger imagination. Imagination, in its turn, enables practicing four dimensions of intimacy: embodied, emotional, daily and imagined.

  16. IMAGINING THE ABSENT PARTNER - INTIMACY AND IMAGINATION IN LONG-DISTANCE RELATIONSHIPS

    Iveta Jurkane-Hobein

    2015-01-01

    The dominant discourse on relationships in modernity argues for the importance of intimacy, including the intimacy of bodies, for the relationship to be continuous. This raises the question as to how couples that cannot meet face-to-face on a regular basis due to geographical distance maintain intimacy during repetitious non-co-presence. In this article, intimacy is seen as a relational quality that is created and maintained by individuals themselves through practices of intimacy (Jamieson, 2...

  17. Evidence for cell fusion is absent in vascular lesions associated with pulmonary arterial hypertension

    Majka, S. M.; M. Skokan; Wheeler, L; Harral, J.; Gladson, S.; Burnham, E.; J. E. Loyd; Stenmark, K R; Varella-Garcia, M; West, J.

    2008-01-01

    Pulmonary arterial hypertension (PAH) is a fatal disease associated with severe remodeling of the large and small pulmonary arteries. Increased accumulation of inflammatory cells and apoptosis-resistant cells are contributing factors. Proliferative apoptosis-resistant cells expressing CD133 are increased in the circulation of PAH patients. Circulating cells can contribute to tissue repair via cell fusion and heterokaryon formation. We therefore hypothesized that in the presence of increased l...

  18. Face to face with the absent Buddha : The formation of Buddhist Aniconic art

    Karlsson, Klemens

    2000-01-01

    Early art in Buddhist cultic sites was characterized by the absence of anthropomorphicimages of the Buddha. The Buddha was instead represented by different signs, like awheel, a tree, a seat and footprints. This study emphasizes the transformation this artunderwent from simple signs to carefully made aniconic compositions representing theBuddha in a narrative context. Buddhist aniconic art has been explained by a prohibition against images of theBuddha or by a doctrine that made it inappropri...

  19. Meagre escapees: consequences of a local-absent farmed species in the W-Mediterranean

    Pablo Arechavala-Lopez

    2015-11-01

    Full Text Available Meagre (Argyrosomus regius Asso, 1810 is a large sciaenid considered an emerging species in the European aquaculture, especially for W-Mediterranean countries. Meagre production has grown rapidly in recent years, being Spain, Turkey, Italy and France are the main meagre producer countries in the world, but it is also cultivated on small scale in other Mediterranean countries, such as Malta, Croatia or Morocco. However, farmed meagre might escape from net-pens of coastal floating facilities leading to economic losses for farmers, but also causing environmental problems on coastal ecosystems. The geographic distribution and genetic fragmentation of meagre native populations suggest that farmed meagre should be considered nowadays as “local-absent” fish in most of the Mediterranean regions where it is being farmed. Therefore, the potential risks of escaped meagre might be higher than those reported for native species, although often unpredictable. Meagre escapees might spread diseases and pathogens to nearby farms or local fish populations, competing for natural resources (food and habitat and predating on other species. Consequently, the interactions of escaped fish with wild populations might have also indirect socio-economic effects on local fisheries and consumers. Nevertheless, escape events from meagre aquaculture and they derived environmental and socio-economic interactions have been scarcely reported up today, and therefore, further studies are highly needed to understand and evaluate the potential consequences of what is being farmed in the Mediterranean.

  20. How Absent Negativity Relates to Affect and Motivation: An Integrative Relief Model

    Roland eDeutsch; Kevin eSmith; Robert eKordts-Freudinger; Regina eReichardt

    2015-01-01

    The present paper concerns the motivational underpinnings and behavioral correlates of the prevention or stopping of negative stimulation – a situation referred to as relief. Relief is of great theoretical and applied interest. Theoretically, it is tied to theories linking affect, emotion and motivational systems (Carver & Scheier, 1990; Gray & McNaughton, 2000; Higgins, 1997; Lang, Bradley, & Cuthbert, 1990). Importantly, these theories make different predictions regarding the association be...

  1. How absent negativity relates to affect and motivation: an integrative relief model.

    Deutsch, Roland; Smith, Kevin J M; Kordts-Freudinger, Robert; Reichardt, Regina

    2015-01-01

    The present paper concerns the motivational underpinnings and behavioral correlates of the prevention or stopping of negative stimulation - a situation referred to as relief. Relief is of great theoretical and applied interest. Theoretically, it is tied to theories linking affect, emotion, and motivational systems. Importantly, these theories make different predictions regarding the association between relief and motivational systems. Moreover, relief is a prototypical antecedent of counterfactual emotions, which involve specific cognitive processes compared to factual or mere anticipatory emotions. Practically, relief may be an important motivator of addictive and phobic behaviors, self destructive behaviors, and social influence. In the present paper, we will first provide a review of conflicting conceptualizations of relief. We will then present an integrative relief model (IRMO) that aims at resolving existing theoretical conflicts. We then review evidence relevant to distinctive predictions regarding the moderating role of various procedural features of relief situations. We conclude that our integrated model results in a better understanding of existing evidence on the affective and motivational underpinnings of relief, but that further evidence is needed to come to a more comprehensive evaluation of the viability of IRMO. PMID:25806008

  2. How Absent Negativity Relates to Affect and Motivation: An Integrative Relief Model

    Roland eDeutsch

    2015-03-01

    Full Text Available The present paper concerns the motivational underpinnings and behavioral correlates of the prevention or stopping of negative stimulation – a situation referred to as relief. Relief is of great theoretical and applied interest. Theoretically, it is tied to theories linking affect, emotion and motivational systems (Carver & Scheier, 1990; Gray & McNaughton, 2000; Higgins, 1997; Lang, Bradley, & Cuthbert, 1990. Importantly, these theories make different predictions regarding the association between relief and motivational systems. Moreover, relief is a prototypical antecedent of counterfactual emotions, which involve specific cognitive processes compared to factual or mere anticipatory emotions. Practically, relief may be an important motivator of addictive and phobic behaviors (Mowrer, 1951; Ostafin & Brooks, 2011, self destructive behaviors (Favazza, 1998; Franklin, Lee, Hanna, & Prinstein, 2013, and social influence (Dolinski & Nawrat, 1998. In the present paper, we will first provide a review of conflicting conceptualizations of relief. We will then present an integrative relief model (IRMO that aims at resolving existing theoretical conflicts. We then review evidence relevant to distinctive predictions regarding the moderating role of various procedural features of relief situations. We conclude that our integrated model results in a better understanding of existing evidence on the affective and motivational underpinnings of relief, but that further evidence is needed to come to a more comprehensive evaluation of the viability of IRMO.

  3. How absent negativity relates to affect and motivation: an integrative relief model

    Deutsch, Roland; Smith, Kevin J. M.; Kordts-Freudinger, Robert; Reichardt, Regina

    2015-01-01

    The present paper concerns the motivational underpinnings and behavioral correlates of the prevention or stopping of negative stimulation – a situation referred to as relief. Relief is of great theoretical and applied interest. Theoretically, it is tied to theories linking affect, emotion, and motivational systems. Importantly, these theories make different predictions regarding the association between relief and motivational systems. Moreover, relief is a prototypical antecedent of counterfa...

  4. Pendrin and NIS antibodies are absent in healthy individuals and are rare in autoimmune thyroid disease

    Brix, Thomas H; Hegedüs, Laszlo; Weetman, Anthony P;

    2014-01-01

    OBJECTIVE: Antibodies against thyroglobulin, thyroid peroxidase and the TSH receptor are accepted as pathophysiological and diagnostic biomarkers in autoimmune thyroid disease (AITD). In contrast, the prevalence, aetiology and clinical relevance of autoantibodies against the human sodium-iodine s......OBJECTIVE: Antibodies against thyroglobulin, thyroid peroxidase and the TSH receptor are accepted as pathophysiological and diagnostic biomarkers in autoimmune thyroid disease (AITD). In contrast, the prevalence, aetiology and clinical relevance of autoantibodies against the human sodium......-iodine symporter (NISAb) and pendrin (PenAb) remain unclear. The objectives of the study were to investigate the presence of NISAb and PenAb in Danish twins, with and without AITD, to study whether the published variations in NISAb and PenAb frequencies were related to differences in methodology or study...... populations, and to evaluate whether the presence of NISAb or PenAb most likely results from genetic or nongenetic factors. METHODS: Sera from 93 patients with AITD and 230 healthy controls were evaluated for NISAb and PenAb using radioligand binding assays (RBA). RESULTS: Patients with AITD had a higher...

  5. Absent cavum septum pellucidum: a review with emphasis on associated commissural abnormalities

    Sundarakumar, Dinesh K.; Farley, Sarah A.; Nixon, Jason N. [Seattle Children' s Hospital, Department of Radiology, Seattle, WA (United States); Smith, Crysela M. [The University of Texas Health Science Center at San Antonio, Department of Radiology, San Antonio, TX (United States); Maravilla, Kenneth R.; Dighe, Manjiri K. [University of Washington, Department of Radiology, Seattle, WA (United States)

    2015-07-15

    The cavum septum pellucidum (CSP) is an important fetal midline forebrain landmark, and its absence often signifies additional underlying malformations. Frequently detected by prenatal sonography, absence of the CSP requires further imaging with pre- or postnatal MRI to characterize the accompanying abnormalities. This article reviews the developmental anatomy of the CSP and the pivotal role of commissurization in normal development. An understanding of the patterns of commissural abnormalities associated with absence of the CSP can lead to improved characterization of the underlying spectrum of pathology. (orig.)

  6. Evidence that urocortin is absent from neurons of the Edinger-Westphal nucleus in pigeons

    Cavani J.A.

    2003-01-01

    Full Text Available The Edinger-Westphal nucleus (EWN is a central preganglionic parasympathetic cell group that gives rise to cholinergic input to the ciliary ganglion, thereby regulating several neurovegetative ocular functions. Recently, the supposed presence of the neuropeptide urocortin (UCN has been reported in EWN neurons in rodent brain. The purpose of the present study was to examine the distribution of UCN in avian brain and to investigate by immunohistochemical analysis the possible use of this substance as an EWN marker in a non-mammalian class of vertebrates. Brain tissue of pigeons was incubated with a specific antibody against UCN and the results showed labeling of many small neurons, forming a double wing in the dorsal mesodiencephalic transition area. Their size and shape, however, differed from those of EWN neurons, and they were preferentially located rostral to the EWN. Double-label experiments employing an antibody against the enzyme choline acetyltransferase (ChAT showed that UCN is not localized to the cholinergic cells of the EWN and confirmed the rostral distributionof UCN never overlapping the ChAT+ EWN cells. Taken together, these results suggest that, at least in pigeons, the UCN+ population does not belong to the traditionally defined EWN.

  7. Exaggerated object affordance and absent automatic inhibition in alien hand syndrome

    Jennifer McBride, Petroc Sumner, Stephen R. Jackson, Nin Bajaj, & Masud Husain

    2013-01-01

    Patients with alien hand syndrome (AHS) experience making apparently deliberate and purposeful movements with their hand against their will. However, the mechanisms contributing to these involuntary actions remain poorly understood. Here, we describe two experimental investigations in a patient with corticobasal syndrome (CBS) with alien hand behaviour in her right hand. First, we show that responses with the alien hand are made significantly more quickly to images of objects which afford an ...

  8. Immunological effects of low dose radiation. Absent or minor effects of Chernobyl fallout in Norway?

    Reitan, J.B.; Bergan, T.D.; Strand, P. [Statens Straalevern, Oesteraas (Norway); Melbye, O.J. [Rikshospitalet, Oslo (Norway)

    1998-01-01

    In this pilot study of those Norwegian individuals most heavily exposed to the Chernobyl Fallout, immunological parameters generally stayed within normal limits. However, some parameter, apparently within the assumed normal range did, in fact correlate to the estimated individual dose as assessed by wholebody counting of radiocaesium content. The small possible effects revealed in this study may represent real biological effects, but do not necessarily represent a health detriment. 43 refs., 4 figs., 6 tabs.

  9. Heavy-light, absent-present: rethinking the 'weight' of imprisonment.

    Crewe, Ben; Liebling, Alison; Hulley, Susie

    2014-09-01

    Since King and McDermott (1995), following Downes (1988), defined the psychological oppressiveness of incarceration in terms of 'weight', little has been written about the 'weight of imprisonment'. None the less, it is generally assumed that prisons that are 'light' are preferable to those that are 'heavy' - in part because of an assumption among many penologists that power, and its application, is dangerous and antagonistic. This article does not dispute that 'heavy' prisons are undesirable. Its argument is that there can also be dangers if prisons are excessively light. Many of these dangers are linked to the under-use of power. The tone and quality of prison life depends on the combined effects of institutional weight with the 'absence' or 'presence' of staff power. Drawing on prisoners' descriptions of their experiences in public and private sector prisons, and their assessments of important aspects of their quality of life, the article outlines what these concepts mean in practice. The authors develop a four-quadrant framework for conceptualizeng penal legitimacy and the experience of penal authority. PMID:25251137

  10. Adaptive associations between social cognition and emotion regulation are absent in schizophrenia and bipolar disorder

    Jesseca Elise Rowland

    2013-01-01

    Full Text Available Schizophrenia (SZ and bipolar disorder (BD are associated with impairments in facial emotion perception and Theory of Mind (ToM. These social cognitive skills deficits may be related to a reduced capacity to effectively regulate one’s own emotions according to the social context. We therefore set out to examine the relationship between social cognitive abilities and the use of cognitive strategies for regulating negative emotion in SZ and BD. Participants were 56 SZ, 33 BD, and 58 healthy controls (HC who completed the Ekman 60-faces test of facial emotion recognition; a sub-set of these participants also completed The Awareness of Social Inference Test (TASIT and the Cognitive Emotion Regulation Questionnaire (CERQ. SZ participants demonstrated impairments in emotion perception on both the Ekman and the TASIT Emotion Evaluation tests relative to BD and HC. While both SZ and BD patients showed ToM deficits (i.e., perception of sarcasm and lie compared to HC, SZ patients demonstrated significantly greater ToM impairment compared to BD. There were also distinct patterns of cognitive strategies used to regulate emotion in both patient groups: those with SZ were more likely to engage in catastrophising and rumination, while BD subjects were more likely to blame themselves and were less likely to engage in positive reappraisal, relative to HC. In addition, those with SZ were more likely to blame others compared to BD. Associations between social cognition and affect regulation were revealed for HC only: TASIT performance was negatively associated with more frequent use of rumination, catastrophising and blaming others, such that more frequent use of maladaptive cognitive emotion regulation strategies was associated with poor social cognitive performance. These associations were not present in either patient group. However, both SZ and BD patients demonstrated poor ToM performance and aberrant use of emotion regulation strategies consistent with previous studies.