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Sample records for abo incompatible caso

  1. ABO-incompatible kidney transplantation

    Schousboe, Karoline; Titlestad, Kjell; Baudier, Francois;

    2010-01-01

    INTRODUCTION: Kidney transplantation is the optimal treatment for many patients with end-stage renal disease (ESRD). Due to shortage of donor kidneys in Denmark, there is a need to expand the possibilities for donation. At the Odense University Hospital (OUH), we have introduced ABO-incompatible ......INTRODUCTION: Kidney transplantation is the optimal treatment for many patients with end-stage renal disease (ESRD). Due to shortage of donor kidneys in Denmark, there is a need to expand the possibilities for donation. At the Odense University Hospital (OUH), we have introduced ABO......-incompatible kidney transplantation. We used antigenspecific immunoadsorptions to remove blood group antibodies and anti-CD20 antibody (rituximab) to inhibit the antibody production. The aim of introducing the ABO-incompatible kidney transplantation at the OUH was to increase the rate of living donor kidney...

  2. ABO incompatibility hemolytic disease following exchange transfusion 96 newborn

    Khatami S.F

    2007-09-01

    Full Text Available Background: ABO incompatibility hemolytic disease of the newborn is a common cause of clinical jaundice and causes two-thirds of the hemolytic disease in newborns. This study was undertaken to determine the frequency of ABO incompatibility hemolytic disease and its complications in newborns undergoing exchange transfusion.Methods: This prospective and descriptive study was performed in jaundiced newborn infants during a three-year period. Inclusion criteria were: maternal blood type O, newborn blood type A or B, rising indirect hyperbilirubinemia in the first two days of life, positive immunohematologic test for newborns and exchange transfusion. Exclusion criteria were: incomplete information, other accompanying diseases that induce hyperbilirubinemia. All newborn infants received phototherapy before and after exchange transfusion. We did not use intravenous immunoglobulin, hemoxygenase inhibitor drugs and blood products before exchange transfusion.Results: Double-volume exchange transfusion via umbilical cord catheter was performed in 96 patients, 19 (20% of whom suffered from ABO incompatibility. Of these 19 newborns, two-thirds (13 were preterm infants. The minimum level of serum bilirubin was 10 mg/dl and the maximum serum bilirubin level was 35 mg/dl. In six patients (32% serum bilirubin levels were >25mg/dl. The most common blood group was type A for newborns. Immunohematologic tests were positive in 84% of the mothers. ABO incompatibility hemolytic disease was the fourth and second most common reasons for blood exchange transfusion in preterm and term infants, respectively. Laboratory complications were more common than clinical complications. The etiology of 48% of the alloimmunization and 42% of the hemolytic disease in these newborns was ABO incompatibility.Conclusions: Mothers with blood group O and newborns with blood group A or B with positive immunohematologic tests in first hours of life are at high risk for hemolytic disease

  3. ABO-INCOMPATIBLE PEDIATRIC LIVER TRANSPLANTATION: THE ANALYSIS OF WORLD EXPERIENCE

    I. E. Tsirulnikova

    2012-12-01

    Full Text Available In the review we present the world scientific literature data analysis of the results of ABO-incompatible liver transplantation in children and adults. Used previously and modern protocols of pre- and postoperative manage- ment of ABO-incompatible recipients from different transplant centers are described. While adult ABO-incom- patible liver transplantation is still linked with increased risk of immune complications, short and long-term results of ABO-incompatible liver transplantation in children are similar to those of ABO-compatible transplan- tation. We conclude that ABO-incompatible liver transplantation is reasonable in urgent cases for recipients of all age groups and for children without possible ABO-compatible living related donors. 

  4. Our first experiences in applying an original method for removal of ABO-isoagglutinins in ABO-incompatible kidney recipients

    Ignjatović Ljiljana

    2009-01-01

    Full Text Available Background/Aim. Due to improved methods for removal of ABO isoagglutinins and novel immunosuppressive protocols, short and long term outcome in blood group incompatible is similar to blood group compatible kidney transplantation. The aim of this study was to determine the efficacy of our original method for removal of ABO isoagglutinins from the blood in ABO-incompatible kidney allograft recipients. Method. Between 2006 and 2008 twelve patients were transplanted from ABO incompatible living donors. Titers of ABO isoagglutinins were 4-128 (IgG. Immunosuppressive therapy started 14 days before kidney transplantation with rituximab, followed by a triple therapy (prednisone + tacrolimus + mycophenolate mofetil and the first plasma exchange (PE procedure, in which one plasma volume was substituted with albumin and saline on day 7 before transplantation. For selective extracorporeal immunoadsorption, the removed plasma was mixed with donor blood type filtered red blood cells, centrifuged and the supernatant separated and preserved. In the next PE procedure, the removed plasma was replaced with immunoadsorbed plasma, and so on. Titers of ABO agglutinins, renal allograft function and survival were followed-up. Results. The pre-transplant treatment consisting of 1-5 PE procedures and immunosuppressive therapy resulted in target ABO agglutinins titers below 4. During a 10-24 month follow-up three patients had an early acute rejection, one patient acute rejection and hemolytic anemia, two patients surgical complications and one of them lost his graft. In the post-transplant period, the titers of ABO antibodies remained below 4. All the patients had stable kidney allograft function with mean serum creatinine ±SD of 129 ± 45 μmol/l at the end of the study. Conclusion. Our method for removal of ABO antibodies was effective in a limited series of patients and short-term follow-up.

  5. ABO Incompatible Liver Transplantation as a Bridge to Treat HELLP Syndrome

    Brad Butler

    2009-01-01

    Full Text Available The following is a case report of a primiparous woman who developed fulminant liver failure in the setting of HELLP syndrome complicated by hepatic rupture. It is unique in that a timely ABO compatible liver donor was unavailable, necessitating the transplantation of an ABO incompatible organ. Despite aggressive therapy, severe reperfusion injury and humoral rejection dictated retransplantation with an ABO compatible organ on postoperative day 15, resulting in rapid clinical recovery.

  6. Risk factor for ischemic-type biliary lesion after ABO-incompatible living donor liver transplantation

    Bang, Jun Bae; Kim, Bong-Wan; Kim, Young Bae; Wang, Hee-Jung; Lee, Hyun Yeong; Sim, Joohyun; Kim, Taegyu; Lee, Kyeong Lok; Hu, Xu-Guang; Mao, Wei

    2016-01-01

    AIM: To evaluate the risk factors for ischemic-type biliary lesion (ITBL) after ABO-incompatible (ABO-I) adult living donor liver transplantation (ALDLT). METHODS: Among 141 ALDLTs performed in our hospital between 2008 and 2014, 27 (19%) were ABO-I ALDLT and 114 were ABO-identical/compatible ALDLT. In this study, we extensively analyzed the clinico-pathological data of the 27 ABO-I recipients to determine the risk factors for ITBL after ABO-I ALDLT. All ABO-I ALDLT recipients underwent an identical B-cell depletion protocol with preoperative rituximab, plasma exchange (PE), and operative splenectomy. The median follow-up period after transplantation was 26 mo. The clinical outcomes of the 27 ABO-I ALDLT recipients were compared with those of 114 ABO-identical/compatible ALDLT recipients. RESULTS: ITBL occurred in four recipients (14.8%) between 45 and 112 d after ABO-I ALDLT. The overall survival rates were not different between ABO-I ALDLT and ABO-identical/compatible ALDLT (P = 0.303). Among the ABO-I ALDLT recipients, there was no difference between patients with ITBL and those without ITBL in terms of B-cell and T-cell count, serum isoagglutinin titers, number of PEs, operative time and transfusion, use of graft infusion therapy, or number of remnant B-cell follicles and plasma cells in the spleen. However, the perioperative NK cell counts in the blood of patients with ITBL were significantly higher than those in the patients without ITBL (P 150/μL and postoperative NK cell count > 120/μL were associated with greater relative risks (RR) for development of ITBL (RR = 20 and 14.3, respectively, P transplant recipient’s blood are associated with ITBL after ABO-I ALDLT. Further research is needed to elucidate the molecular mechanism of NK cell involvement in the development of ITBL.

  7. ABH-Glycan Microarray Characterizes ABO Subtype Antibodies: Fine Specificity of Immune Tolerance After ABO-Incompatible Transplantation.

    Jeyakanthan, M; Meloncelli, P J; Zou, L; Lowary, T L; Larsen, I; Maier, S; Tao, K; Rusch, J; Chinnock, R; Shaw, N; Burch, M; Beddows, K; Addonizio, L; Zuckerman, W; Pahl, E; Rutledge, J; Kanter, K R; Cairo, C W; Buriak, J M; Ross, D; Rebeyka, I; West, L J

    2016-05-01

    Organ transplantation from ABO blood group-incompatible (ABOi) donors requires accurate detection, effective removal and subsequent surveillance of antidonor antibodies. Because ABH antigen subtypes are expressed differently in various cells and organs, measurement of antibodies specific for the antigen subtypes in the graft is essential. Erythrocyte agglutination, the century-old assay used clinically, does not discriminate subtype-specific ABO antibodies and provides limited information on antibody isotypes. We designed and created an ABO-glycan microarray and demonstrated the precise assessment of both the presence and, importantly, the absence of donor-specific antibodies in an international study of pediatric heart transplant patients. Specific IgM, IgG, and IgA isotype antibodies to nonself ABH subtypes were detected in control participants and recipients of ABO-compatible transplants. Conversely, in children who received ABOi transplants, antibodies specific for A subtype II and/or B subtype II antigens-the only ABH antigen subtypes expressed in heart tissue-were absent, demonstrating the fine specificity of B cell tolerance to donor/graft blood group antigens. In contrast to the hemagglutination assay, the ABO-glycan microarray allows detailed characterization of donor-specific antibodies necessary for effective transplant management, representing a major step forward in precise ABO antibody detection. PMID:26602221

  8. Adult Living Donor Liver Transplantation with ABO-Incompatible Grafts: A German Single Center Experience

    Armin D. Goralczyk

    2009-01-01

    Full Text Available Adult living donor liver transplantations (ALDLTs across the ABO blood group barrier have been reported in Asia, North Americas, and Europe, but not yet in Germany. Several strategies have been established to overcome the detrimental effects that are attached with such a disparity between donor and host, but no gold standard has yet emerged. Here, we present the first experiences with three ABO-incompatible adult living donor liver transplantations in Germany applying different immunosuppressive strategies. Four patient-donor couples were considered for ABO-incompatible ALDLT. In these patients, resident ABO blood group antibodies (isoagglutinins were depleted by plasmapheresis or immunoadsorption and replenishment was inhibited by splenectomy and/or B-cell-targeted immunosuppression. Despite different treatments ALDLT could safely be performed in three patients and all patients had good initial graft function without signs for antibody-mediated rejection (AMR. Two patients had long-term graft survival with stable graft function. We thus propose the feasibility of ABO-incompatible ALDLT with these protocols and advocate further expansion of ABO incompatible ALDLT in multicenter trials to improve efficacy and safety.

  9. Late antibody-mediated rejection after ABO-incompatible kidney transplantation during Gram-negative sepsis

    2014-01-01

    Background The major challenge in ABO-incompatible transplantation is to minimize antibody-mediated rejection. Effective reduction of the anti-ABO blood group antibodies at the time of transplantation has made ABO-incompatible kidney transplantation a growing practice in our hospital and in centers worldwide. ABO antibodies result from contact with A- and B-like antigens in the intestines via nutrients and bacteria. We demonstrate a patient with fulminant antibody-mediated rejection late after ABO-incompatible kidney transplantation, whose anti-A antibody titers rose dramatically following Serratia marcescens sepsis. Case presentation A 58-year-old woman underwent an ABO-incompatible kidney transplantation for end-stage renal disease secondary to autosomal dominant polycystic kidney disease. It concerned a blood group A1 to O donation. Pre-desensitization titers were 64 for anti-blood group A IgM and 32 for anti-blood group A IgG titers. Desensitization treatment consisted of rituximab, tacrolimus, mycophenolate mofetil, corticosteroids, immunoadsorption and intravenous immunoglobulines. She was readmitted to our hospital 11 weeks after transplantation for S. marcescens urosepsis. Her anti-A IgM titer rose to >5000 and she developed a fulminant antibody-mediated rejection. We hypothesized that the (overwhelming) presence in the blood of S. marcescens stimulated anti-A antibody formation, as S. marcescens might share epitopes with blood group A antigen. Unfortunately we could not demonstrate interaction between blood group A and S. marcescens in incubation experiments. Conclusion Two features of this post-transplant course are remarkably different from other reports of acute rejection in ABO-incompatible kidney transplantation: first, the late occurrence 12 weeks after kidney transplantation and second, the very high anti-A IgM titers (>5000), suggesting recent boosting of anti-A antibody formation by S. marcescens. PMID:24517251

  10. Clinico-serologic co-relation in bi-directional ABO incompatible hemopoietic stem cell transplantation

    Sabita Basu

    2015-01-01

    Full Text Available Background: The ABO blood group system is of prime significance in red cell transfusion and organ transplantation. However, ABO compatibility is not critical in allogenic hemopoietic stem cell transplantation (HSCT and approximately 40-50% of hemopoietic stem cell transplants are ABO incompatible. This incompatibility may be major, minor or bi-directional. Though there are descriptions of transfusion practice and protocols in ABO incompatible HSCT, there are considerable variations and transfusion support in these patients can be very challenging. Aims: The immunohematologic observations in two cases of bi-directional ABO incompatible HSCT have been described, and clinico-serologic correlation has been attempted. Materials and Methods: In both cases, peripheral blood stem cell harvests were obtained using the Cobe spectra cell separator. Immunohematologic assessments in the donor and recipient were done as a part of pre HSCT evaluation. Both the standard tube technique and column agglutination method (Ortho Biovue Micro Bead System was used. Antibody screen was done by column agglutination method using three cell panel (Surgiscreen cells. Isoagglutinin titration was done by the master dilution method and standard validated techniques were used. Results: The pattern of laboratory findings in the two cases was different and so were the clinical outcomes. Although there was early engraftment in the first case, the second case developed pure red cell aplasia and this was well-reflected in the immunohematologic assessments. Conclusion: Immunohematologic assessment correlated well with the clinical picture and could be used to predict clinical outcome and onset of complications in ABO incompatible HSCT.

  11. Early post-transplant complications following ABO-incompatible kidney transplantation

    Naciri Bennani, Hamza; Abdulrahman, Zhyiar; Allal, Asma; Sallusto, Federico; Delarche, Antoine; Game, Xavier; Esposito, Laure; Doumerc, Nicolas; Debiol, Bénédicte; Kamar, Nassim; Rostaing, Lionel

    2016-01-01

    Background: Living-kidney transplantation is increasing because of the scarcity of kidneys from deceased donors and the increasing numbers of patients on waiting lists for a kidney transplant. Living-kidney transplantation is now associated with increased long-term patient- and allograft-survival rates. Objectives: The purpose of this retrospective study was to identify, in a cohort of 44 ABO-incompatible (ABOi) live-kidney transplant patients, the main complications that occurred within 6 months post-transplantation, and to compare these findings with those from 44 matched ABO-compatible (ABOc) live-kidney transplant patients who were also from our center. Patients and Methods: This single-center retrospective study assessed post-transplantation complications in 44 ABO-i versus 44 matched ABO-c patients. All patients were comparable at baseline except that ABO-i patients had greater immunological risks. Results: During the 6-month post-transplant period, more ABO-i patients presented with postoperative bleeds, thus requiring significantly more blood transfusions. Bleeds were associated with significantly lower values of fibrinogen, platelets, prothrombin time, and hemoglobin levels. Surgical complications, patient- and graft-survival rates, and kidney-function statuses were similar between both groups at 6 months post-transplantation. Conclusions: We conclude that impairment of hemostatic factors at pre-transplant explained the increased risk of a post-transplant bleed in ABO-i patients. PMID:27047806

  12. Micro gel column technique is fit for detecting mixed fields post ABO incompatible hematopoietic stem cell transplantation.

    Li, Min-Fang; Liu, Feng; Zhang, Min

    2015-04-01

    How to choose suitable serologic method for assessment of the actual stages of ABO chimera is more important to establish transfusion strategy for patients post-ABO incompatible hematopoietic stem cell transplantation. We reported ABO phenotypes of a patient post-ABO minor incompatible hematopoietic stem cell transplantation from 1+ weak agglutination by tube method was obviously reaffirmed to mixed fields with 4+ positive reaction by micro gel column card. Hence, blood bank technologists must continually work together with hematologist to establish appropriate transfusion strategy, and micro gel column technique can be more appropriate for detecting mixed fields during the whole period of transplantation. PMID:25578650

  13. Acute antibody-mediated rejection after ABO-incompatible kidney transplantation treated successfully with antigen-specific immunoadsorption

    Just, Søren Andreas; Marcussen, Niels; Sprogøe, Ulrik; Koefoed-Nielsen, Pernille; Bistrup, Claus

    2010-01-01

    ABO-incompatible kidney transplantation is possible after pre-treatment with rituximab, intravenous immunoglobulin and basiliximab combined with tacrolimus, mycophenolate mofetil and prednisolone. We report on the first patient treated with this protocol who developed acute antibody-mediated reje...... that ABO-incompatible kidney transplantation complicated by acute antibody-mediated rejection, caused by ABO antibodies, may successfully be treated with this regime.......ABO-incompatible kidney transplantation is possible after pre-treatment with rituximab, intravenous immunoglobulin and basiliximab combined with tacrolimus, mycophenolate mofetil and prednisolone. We report on the first patient treated with this protocol who developed acute antibody......-mediated rejection (Banff grade II with IgG deposits) caused by ABO antibodies (anti-B). Anti-rejection treatment with anti-B-specific immunoadsorption, intravenous immunoglobulin and methylprednisolone efficiently cleared deposited IgG from the kidney allograft and re-established normal kidney function. We suggest...

  14. Aplasia pura de serie roja post-trasplante alogeneico de células progenitoras hematopoyeticas ABO incompatible Pure red cell aplasia after ABO incompatible bone marrow transplantation

    E. Bulliorsky

    2002-12-01

    Full Text Available El trasplante alogeneico de células progenitoras hematopoyéticas (TCPH con incompatibilidad ABO entre el donante y el receptor puede en ocasiones asociarse a trastornos en la progenie eritroide desarrollada a partir de la médula ósea trasplantada, caracterizado por un funcionamiento tardío, inadecuado e incompleto de la misma. En este contexto, la aplasia pura de serie roja es la complicación más severa. Se han intentado tratamientos para la aplasia pura de serie roja post-TCPH con eritropoyetina o plasmaféresis, con relativo éxito. Algunos autores han informado también la utilización de globulina antilinfocitaria, asumiendo que dicha aplasia selectiva de la serie roja en la médula ósea trasplantada es mediada por un mecanismo inmune. En este trabajo se describe un paciente portador de una leucemia aguda en quien se realizó un TCPH alogeneico (ABO incompatible con su donante. Teniendo niveles bajos de aglutininas contra el grupo sanguíneo de la donante, desarrolló una aplasia pura de serie roja post - TCPH. La misma no mejoró con tratamiento con eritropoyetina o con un refuerzo de progenitores hematopoyéticos de sangre periférica de la misma donante (boost, resolviéndose totalmente luego de un tratamiento exitoso con globulina antilinfocitaria de origen equino.ABO incompatibility in allogeneic bone marrow transplantation may be associated with incomplete or delayed erythroid engraftment, being pure red cell aplasia (PRCA the most severe complication in this setting. Attempts for the treatment of PRCA have been made with erythropoietin or with plasmapheresis with relative success, and some authors have reported the reversibility of PRCA with antilymphocyte globulin (ALG or ATG, based on the assumption that PRCA might be immunologically mediated. We report herewith a patient with acute leukemia who developed post - BMT pure red cell aplasia. His sibling donor (sister was HLA identical and ABO incompatible, having low agglutinin

  15. ABO-incompatible kidney transplant recipients have a higher bleeding risk after antigen-specific immunoadsorption.

    de Weerd, Annelies E; van Agteren, Madelon; Leebeek, Frank W; Ijzermans, Jan N M; Weimar, Willem; Betjes, Michiel G H

    2015-01-01

    Pretransplant removal of antiblood group ABO antibodies is the cornerstone of all current ABO-incompatible (ABOi) transplantation programmes. In our protocol, plasmapheresis (PP) is performed with a plasmafilter followed by immunoadsorption (IA) of anti-ABO antibodies. The bleeding complications of this technique are not known. We analysed the data of all 65 consecutive ABOi kidney transplantations between March 2006 and October 2013 and compared these with matched 130 ABO-compatible (ABOc) kidney transplantations. Cases differed from controls in the pre-operative regimen, which included IA-PP and rituximab, tacrolimus, mycophenolate mofetil, prednisone and immunoglobulines. Data on platelet count, blood loss and red blood cell (EC) transfusions during 48 h postoperatively were collected. ABOi patients received EC transfusions more frequently than controls (29% vs. 12%, P = 0.005). Intra-operative blood loss was higher (544 vs. 355 ml, P < 0.005) and they experienced more major bleeding (≥3 EC within 24 h, 15% vs. 2%, P < 0.0005). Platelet count decreased by 28% after the pre-operative IA. In a multivariate model, only the number of pre-operative IAs was associated with the number of ECs given (OR per IA 1.9, P < 0.05). ABOi kidney transplant recipients have a high postoperative bleeding risk, correlating with the number of pre-operative IA sessions performed. PMID:25070762

  16. Different sensitivity of rituximab-treatment to B-cells between ABO-incompatible kidney and liver transplantation.

    Morimoto, Hiroshi; Ide, Kentaro; Tanaka, Yuka; Ishiyama, Kohei; Ohira, Masahiro; Tahara, Hiroyuki; Akita, Tomonori; Tanaka, Junko; Ohdan, Hideki

    2016-06-01

    A desensitization protocol with rituximab is currently widely used for kidney transplantation (KT) and liver transplantation (LT) across the ABO blood group-incompatible (ABO-I) barrier. However, it remains to be elucidated whether rituximab is equally effective for B-cell and T-cell immune responses in both KT and LT recipients. To clarify these effects of rituximab, we enrolled 46 KT and 77 LT recipients in this study. The proportion of peripheral blood B-cells was determined at the perioperative period. T-cell responses to allostimulation were evaluated by a mixed lymphocyte reaction (MLR) assay. One week after rituximab administration, peripheral B-cells became undetectable in ABO-I KT recipients but remained detectable in some of the ABO-I LT recipients; B-cells were undetectable in both groups by week 2. B-cells remained below the detection limit throughout the first year in the ABO-I KT recipients, whereas they reappeared in the periphery after 6months in the ABO-I LT recipients. There were no significant differences in alloreactive T-cell responses based on MLR analyses between ABO-I and ABO-compatible groups. This study indicates that rituximab has differing B-cell sensitivity between KT and LT recipients and a minimal effect on the alloreactive T-cell responses in KT and LT recipients. PMID:27085793

  17. Current trends in platelet transfusions practice: The role of ABO-RhD and human leukocyte antigen incompatibility

    Serena Valsami

    2015-01-01

    Full Text Available Platelet transfusions have contributed to the revolutionary modern treatment of hypoproliferative thrombocytopenia. Despite the long-term application of platelet transfusion in therapeutics, all aspects of their optimal use (i.e., in cases of ABO and/or Rh (D incompatibility have not been definitively determined yet. We reviewed the available data on transfusion practices and outcome in ABO and RhD incompatibility and platelet refractoriness due to anti-human leukocyte antigen (HLA antibodies. Transfusion of platelets with major ABO-incompatibility is related to reduced posttransfusion platelet (PLT count increments, compared to ABO-identical and minor, but still are equally effective in preventing clinical bleeding. ABO-minor incompatible transfusions pose the risk of an acute hemolytic reaction of the recipient that is not always related to high anti-A, B donor titers. ABO-identical PLT transfusion seems to be the most effective and safest therapeutic strategy. Exclusive ABO-identical platelet transfusion policy could be feasible, but alternative approaches could facilitate platelet inventory management. Transfusion of platelets from RhD positive donors to RhD negative patients is considered to be effective and safe though is associated with low rate of anti-D alloimmunization due to contaminating red blood cells. The prevention of D alloimmunization is recommended only for women of childbearing age. HLA alloimmunization is a major cause of platelet refractoriness. Managing patients with refractoriness with cross-matched or HLA-matched platelets is the current practice although data are still lacking for the efficacy of this practice in terms of clinical outcome. Leukoreduction contributes to the reduction of both HLA and anti-D alloimmunization.

  18. Current trends in platelet transfusions practice: The role of ABO-RhD and human leukocyte antigen incompatibility.

    Valsami, Serena; Dimitroulis, Dimitrios; Gialeraki, Argyri; Chimonidou, Maria; Politou, Marianna

    2015-01-01

    Platelet transfusions have contributed to the revolutionary modern treatment of hypoproliferative thrombocytopenia. Despite the long-term application of platelet transfusion in therapeutics, all aspects of their optimal use (i.e., in cases of ABO and/or Rh (D incompatibility) have not been definitively determined yet. We reviewed the available data on transfusion practices and outcome in ABO and RhD incompatibility and platelet refractoriness due to anti-human leukocyte antigen (HLA) antibodies. Transfusion of platelets with major ABO-incompatibility is related to reduced posttransfusion platelet (PLT) count increments, compared to ABO-identical and minor, but still are equally effective in preventing clinical bleeding. ABO-minor incompatible transfusions pose the risk of an acute hemolytic reaction of the recipient that is not always related to high anti-A, B donor titers. ABO-identical PLT transfusion seems to be the most effective and safest therapeutic strategy. Exclusive ABO-identical platelet transfusion policy could be feasible, but alternative approaches could facilitate platelet inventory management. Transfusion of platelets from RhD positive donors to RhD negative patients is considered to be effective and safe though is associated with low rate of anti-D alloimmunization due to contaminating red blood cells. The prevention of D alloimmunization is recommended only for women of childbearing age. HLA alloimmunization is a major cause of platelet refractoriness. Managing patients with refractoriness with cross-matched or HLA-matched platelets is the current practice although data are still lacking for the efficacy of this practice in terms of clinical outcome. Leukoreduction contributes to the reduction of both HLA and anti-D alloimmunization. PMID:26420927

  19. Economic Impacts of ABO-Incompatible Live Donor Kidney Transplantation: A National Study of Medicare-Insured Recipients.

    Axelrod, D; Segev, D L; Xiao, H; Schnitzler, M A; Brennan, D C; Dharnidharka, V R; Orandi, B J; Naik, A S; Randall, H; Tuttle-Newhall, J E; Lentine, K L

    2016-05-01

    The infrequent use of ABO-incompatible (ABOi) kidney transplantation in the United States may reflect concern about the costs of necessary preconditioning and posttransplant care. Medicare data for 26 500 live donor kidney transplant recipients (2000 to March 2011), including 271 ABOi and 62 A2-incompatible (A2i) recipients, were analyzed to assess the impact of pretransplant, transplant episode and 3-year posttransplant costs. The marginal costs of ABOi and A2i versus ABO-compatible (ABOc) transplants were quantified by multivariate linear regression including adjustment for recipient, donor and transplant factors. Compared with ABOc transplantation, patient survival (93.2% vs. 88.15%, p = 0.0009) and death-censored graft survival (85.4% vs. 76.1%, p spending (marginal costs compared to ABOc - year 1: $25 044; year 2: $10 496; year 3: $7307; p spending are easily justified by avoiding long-term dialysis and its associated morbidity and cost. PMID:26603690

  20. Antibody-mediated rejection after ABO-incompatible pediatric living donor liver transplantation for propionic acidemia: A case report.

    Honda, Masaki; Sakamoto, Seisuke; Sakamoto, Rieko; Matsumoto, Shirou; Irie, Tomoaki; Uchida, Koushi; Shimata, Keita; Kawabata, Seiichi; Isono, Kaori; Hayashida, Shintaro; Yamamoto, Hidekazu; Endo, Fumio; Inomata, Yukihiro

    2016-09-01

    We herein present the case of a four-yr-old boy with PA who developed AMR after ABO-incompatible LDLT despite undergoing B cell desensitization using rituximab. Although the CD19+ lymphocyte count decreased to 0.1% nine days after the administration of rituximab, he developed a high fever which was accompanied by arthralgia due to a streptococcal infection 13 days after rituximab prophylaxis. After the clearance of the infection, he underwent ABO-incompatible LDLT 36 days after the administration of rituximab. The CD19+ lymphocyte count just prior to LDLT was 1.2%. He developed AMR five days after LDLT, and the antidonor-type IgM and IgG antibody titers increased to 1:1024 and 1:1024, respectively. He was treated by plasma exchange, IVIG, steroid pulse therapy, and rituximab re-administration; however, his liver dysfunction continued. Despite intensive treatment, he died due to complicated abdominal hernia, acute renal failure, and ARDS. This case suggests that a streptococcal infection may induce the activation of innate immune responses; thus, additional desensitization therapy should be considered prior to ABO-incompatible LDLT if B cell reactivation is suspected. PMID:27436684

  1. The frequency of ABO blood group maternal-fetal incompatibility, maternal iso-agglutinins, and immune agglutinins quantitation in Osogbo, Osun State, South-West of Nigeria

    Oseni Bashiru

    2011-01-01

    Full Text Available Background : ABO incompatibility in maternal-fetal relationship has been shown to cause hemolytic disease of the newborn (HDNB; a survey which is not yet done in this locality. Aim: Frequency of ABO blood group maternal-fetal incompatibility, maternal iso-agglutinins, and immune agglutinins quantitation was carried out in Osogbo, Osun State, South-West of Nigeria. Settings and Designs : A total of 260 subjects comprising 130 postpartum mothers within the age range of 22-35 years having good obstetrics history and normal delivery, with their 130 neonate babies were used for the study. Materials and Methods : ABO cell and serum groupings were carried out on the subjects using standard antisera and cells with appropriate controls. Direct Coomb′s Test was carried out on neonate red cells. Antibody quantitation by double dilution on the maternal serum using red cells containing corresponding antigen to the antibody was determined. A titer, which is the reciprocal of the highest dilution showing agglutination by Indirect Coombs Test, was determined. Another batch of sera was pretreated with 2-mecarptoethanol before determining the titer. Statistical Analysis: The distribution study results obtained were compared in percentages, whereas the antibodies quantitation was expressed as titers using the mode of the titers for compariso-agglutininsn. Results and Conclusions : Thirty-eight percent (50 mothers were ABO incompatible with their babies, whereas 62% (80 mothers were compatible. The distribution of blood groups in the compatible population showed blood group O (45%; A (30%; B (20%; and AB (5%. Mothers O, A, and B carrying incompatible babies had a frequency of 24% each, whereas mothers AB had 28%. Serologist differences occur in maternal ABO antibodies of corresponding incompatible baby ABO antigens. A high incidence of ABO maternal-fetal incompatibility observed without detection of immune agglutinins is indicative of a rare incidence of HDNB due

  2. Atypical hemolytic uremic syndrome diagnosed four years after ABO-incompatible kidney transplantation.

    Kawaguchi, Keiko; Kawanishi, Kunio; Sato, Masayo; Itabashi, Mitsuyo; Fujii, Akiko; Kanetsuna, Yukiko; Huchinoue, Shouhei; Ohashi, Ryuji; Koike, Junki; Honda, Kazuho; Nagashima, Yoji; Nitta, Kosaku

    2015-07-01

    Atypical hemolytic uremic syndrome (aHUS) in allograft kidney transplantation is caused by various factors including rejection, infection, and immunosuppressive drugs. We present a case of a 32 year old woman with aHUS four years after an ABO-incompatible kidney transplantation from a living relative. The primary cause of end-stage renal disease was unknown; however, IgA nephropathy (IgAN) was suspected from her clinical course. She underwent pre-emptive kidney transplantation from her 60 year old mother. The allograft preserved good renal function [serum creatinine (sCr) level 110-130 μmol/L] until a sudden attack of abdominal pain four years after transplant, with acute renal failure (sCr level, 385.3 μmol/L), decreasing platelet count, and hemolytic anemia with schizocytes. On allograft biopsy, there was thrombotic microangiopathy in the glomeruli, with a cellular crescent formation and mesangial IgA and C3 deposition. Microvascular inflammation, such as glomerulitis, peritubular capillaritis, and arteriole endarteritis were also detected. A disintegrin-like and metalloproteinase with thrombospondin type 1 motifs 13 (ADAMTS13) did not decrease and Shiga toxin was not detected. Donor-specific antibodies or autoantibodies, including anti-neutrophil cytoplasmic antibody and anti-glomerular basement membrane (anti-GBM) antibody, were negative. The patient was diagnosed with aHUS and received three sessions of plasmapheresis and methylprednisolone pulse therapy, followed by oral methylprednisolone (0.25-0.5 mg/kg) instead of tacrolimus. She temporarily required hemodialysis (sCr level, 658.3 μmol/L). Thereafter, her sCr level improved to 284.5 μmol/L without dialysis therapy. This case is clinically considered as aHUS after kidney transplantation, associated with various factors, including rejection, glomerulonephritis, and toxicity from drugs such as tacrolimus. PMID:26031589

  3. Antigen-Specific versus Non-Antigen-Specific Immunoadsorption in ABO-Incompatible Renal Transplantation.

    Gerold Thölking

    Full Text Available ABO-incompatible (ABOi renal transplantation (RTx from living donors is an established procedure to expand the donor pool for patients with end stage renal disease. Immunoadsorption (IA is a standard procedure for the removal of preformed antibodies against the allograft. In this study, antigen-specific and non-antigen-specific IA in ABOi RTx were compared.10 patients underwent antigen-specific IA (Glycosorb group and 13 patients non-antigen-specific IA (Immunosorba group. The effects of both procedures regarding antibody reduction, number of treatments, complications, costs, as well as the allograft function and patient survival were compared between both groups.Although the IgG levels were reduced equally by both procedures (p=0.82, the reduction of the IgM level was more effective in the Glycosorb group (p=0.0172. Patients in both groups required a median number of 6 IA before ABOi RTx. Allograft function at one year after AB0i RTx was similar in both groups (estimated glomerular filtration rate: 66 vs. 64 ml/min/1.73m² respectively, with a death-censored graft survival of 90.0% and 92.3% respectively. Complication rates did not differ between procedures. Due to the reuse of non-antigen-specific Immunosorba columns, costs were considerably lower in this group; however, the use of the Immunosorba-based IA was less time-efficient.Considering upcoming alternatives as simultaneous performance of dialysis and IA or a possible reuse of Glycosorb columns, this might become less relevant in the future.

  4. Aplasia pura de serie roja post-trasplante alogeneico de células progenitoras hematopoyeticas ABO incompatible Pure red cell aplasia after ABO incompatible bone marrow transplantation

    E. Bulliorsky; C. Shanley; G. Stemmelin; J. Ceresetto; O. Rabinovich

    2002-01-01

    El trasplante alogeneico de células progenitoras hematopoyéticas (TCPH) con incompatibilidad ABO entre el donante y el receptor puede en ocasiones asociarse a trastornos en la progenie eritroide desarrollada a partir de la médula ósea trasplantada, caracterizado por un funcionamiento tardío, inadecuado e incompleto de la misma. En este contexto, la aplasia pura de serie roja es la complicación más severa. Se han intentado tratamientos para la aplasia pura de serie roja post-TCPH con eritropoy...

  5. Short-Term Outcomes of ABO-Incompatible Living Donor Kidney Transplantation With Uniform Protocol: Significance of Baseline Anti-ABO Titer.

    Lee, K W; Park, J B; Oh, D K; Na, B G; Choi, J Y; Cho, W T; Lee, S H; Park, H J; Cho, D; Huh, W S; Kim, S J

    2016-04-01

    Antibody-mediated rejection (AMR) is one of the major causes of poor outcomes in ABO-incompatible kidney transplantation (ABOi KT). Studies investigating AMR risk factors found that anti-ABO titer is a major issue. However, the significance of antibody titer has been debated. This retrospective study analyzed AMR risk factors in 59 patients who underwent ABOi KT between August 2010 and January 2015. We also analyzed AMR risk factors in recipients with high anti-ABO baseline titers (≥1:64 on dithiothreitol at 37°C phase or ≥1:256 on antihuman globulin phase). The 2-year patient survival rate was 95.8%, and the 2-year graft survival rate was 94.9%. Nine patients (15.3%) experienced clinical (6 of 59 [10.2%]) or subclinical (3 of 59 [5.1%]) AMR. One patient experienced graft loss from hyperacute rejection. AMR risk factor analysis revealed that baseline antibody titer was associated with incidence of AMR. In patients with high baseline titers, low doses of rituximab (200-mg single-dose), an antibody against CD20, was predictive for AMR. Six patients who received pretransplant intravenous immunoglobulin did not experience AMR even when they had high baseline antibody titers. Our results indicate that a high baseline antibody titer affected the incidence of AMR. ABOi KT candidates with high baseline titers need to undergo an intensified preconditioning protocol, including high-dose rituximab (375 mg/m(2)) and intravenous immunoglobulin. PMID:27234744

  6. Delayed hyperacute rejection in a patient who developed clostridium difficile infection after ABO-incompatible kidney transplantation

    Gerald S Lipshutz

    2010-11-01

    Full Text Available Gerald S Lipshutz1, Elaine F Reed2, Phuong-Chi Pham3, Jeffrey M Miller4, Jennifer S Singer5, Gabriel M Danovitch6, Alan H Wilkinson6, Dean W Wallace7, Suzanne McGuire6, Phuong-Truc Pham8, Phuong-Thu Pham61Department of Surgery, Kidney and Pancreas Transplant Program, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA; 2Department of Pathology and Laboratory Medicine-Immunogenetics, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA; 3Department of Medicine, Nephrology Division, UCLA-Olive View Medical Center, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA; 4Department of Medicine, Hematology Oncology Division, UCLA-Olive View Medical Center, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA; 5Department of Surgery and Urology, Kidney and Pancreas Transplant Program, 6Department of Medicine, Nephrology Division, Kidney and Pancreas Transplant Program, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA; 7Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA; 8Department of Science, Penn State University, Worthington-Scranton, Dunmore, PA, USAAbstract: Over the past decade ABO incompatible transplantation has emerged as an important potential source for increasing living kidney transplantation in selected transplant centers. Early reports suggest that patients who have elevated serum anti-blood group antibody titers (anti-A/B before transplantation and a rebound antibody production after antibody removal may be at high immunological risk. With currently available immune modulation protocols and immunosuppressive therapy, excellent short- and long-term patient and graft survival rates have been achieved even in those with high anti-A/B antibody titers before plasmapheresis or immunoadsorption. Nonetheless, acute infection with an organism possessing surface markers analogous to blood group antigens such as carbohydrate structures on

  7. Aplasia pura de serie roja post-trasplante alogeneico de células progenitoras hematopoyeticas ABO incompatible

    E. Bulliorsky

    2002-12-01

    Full Text Available El trasplante alogeneico de células progenitoras hematopoyéticas (TCPH con incompatibilidad ABO entre el donante y el receptor puede en ocasiones asociarse a trastornos en la progenie eritroide desarrollada a partir de la médula ósea trasplantada, caracterizado por un funcionamiento tardío, inadecuado e incompleto de la misma. En este contexto, la aplasia pura de serie roja es la complicación más severa. Se han intentado tratamientos para la aplasia pura de serie roja post-TCPH con eritropoyetina o plasmaféresis, con relativo éxito. Algunos autores han informado también la utilización de globulina antilinfocitaria, asumiendo que dicha aplasia selectiva de la serie roja en la médula ósea trasplantada es mediada por un mecanismo inmune. En este trabajo se describe un paciente portador de una leucemia aguda en quien se realizó un TCPH alogeneico (ABO incompatible con su donante. Teniendo niveles bajos de aglutininas contra el grupo sanguíneo de la donante, desarrolló una aplasia pura de serie roja post - TCPH. La misma no mejoró con tratamiento con eritropoyetina o con un refuerzo de progenitores hematopoyéticos de sangre periférica de la misma donante (boost, resolviéndose totalmente luego de un tratamiento exitoso con globulina antilinfocitaria de origen equino.

  8. A case of passenger lymphocyte syndrome following minor ABO incompatible renal transplantation

    Anju Dubey

    2014-01-01

    Full Text Available Immune hemolysis is one of the adverse effects that can occur following solid organ transplantation. Understanding the clinical settings and the various causes is necessary for prompt diagnosis and appropriate management. One such condition is passenger lymphocyte syndrome (PLS. This case report describes the case of a 27-year-old male renal allograft recipient of the B-positive blood group who received a kidney from an O-positive donor. Postoperatively, the patient showed declining hemoglobin (Hb level and was transfused with B-group packed RBCs (PRBCs, following which there was steep fall in Hb level. A request for PRBCs was sent to the blood bank and this time cross-match with B-group PRBCs showed incompatibility. The patient′s RBCs were found to be strongly DAT (direct anti-globulin test positive and the eluate showed the presence of anti-B with a titer of 32. Thus, diagnosis of probable PLS was made. The patient was managed with methylprednisolone, plasmapheresis and O-group PRBCs. Gradually his condition improved and was discharged in stable condition.

  9. Liver transplantation with deceased ABO-incompatible donors is life-saving but associated with increased risk of rejection and post-transplant complications.

    Thorsen, Trygve; Dahlgren, Ulrika S; Aandahl, Einar Martin; Grzyb, Krzysztof; Karlsen, Tom H; Boberg, Kirsten M; Rydberg, Lennart; Naper, Christian; Foss, Aksel; Bennet, William

    2015-07-01

    ABO-incompatible (ABOi) liver transplantation (LT) with deceased donor organs is performed occasionally when no ABO-compatible (ABOc) graft is available. From 1996 to 2011, 61 ABOi LTs were performed in Oslo and Gothenburg. Median patient age was 51 years (range 13-75); 33 patients were transplanted on urgent indications, 13 had malignancy-related indications, and eight received ABOi grafts for urgent retransplantations. Median donor age was 55 years (range 10-86). Forty-four patients received standard triple immunosuppression with steroids, tacrolimus, and mycophenolate mofetil, and forty-four patients received induction with IL-2 antagonist or anti-CD20 antibody. Median follow-up time was 29 months (range 0-200). The 1-, 3-, 5-, and 10-year Kaplan-Meier estimates of patient survival (PS) and graft survival (GS) were 85/71%, 79/57%, 75/55%, and 59/51%, respectively, compared to 90/87%, 84/79%, 79/73%, and 65/60% for all other LT recipients in the same period. The 1-, 3-, 5-, and 10-year GS for A2 grafts were 81%, 67%, 62%, and 57%, respectively. In conclusion, ABOi LT performed with non-A2 grafts is associated with inferior graft survival and increased risk of rejection, vascular and biliary complications. ABOi LT with A2 grafts is associated with acceptable graft survival and can be used safely in urgent cases. PMID:25736519

  10. Aplasia pura de serie roja post-trasplante alogeneico de células progenitoras hematopoyeticas ABO incompatible

    E. Bulliorsky; C. Shanley; G. Stemmelin; J. Ceresetto; O. Rabinovich

    2002-01-01

    El trasplante alogeneico de células progenitoras hematopoyéticas (TCPH) con incompatibilidad ABO entre el donante y el receptor puede en ocasiones asociarse a trastornos en la progenie eritroide desarrollada a partir de la médula ósea trasplantada, caracterizado por un funcionamiento tardío, inadecuado e incompleto de la misma. En este contexto, la aplasia pura de serie roja es la complicación más severa. Se han intentado tratamientos para la aplasia pura de serie roja post-TCPH con eritropoy...

  11. Frecuencias de grupos sanguíneos e incompatibilidades ABO y RhD, en La Paz, Baja California Sur, México Blood group ABO and RhD frequencies and incompatibilities in La Paz, Baja California Sur, Mexico

    Lorenzo del Peón-Hidalgo

    2002-09-01

    Full Text Available Objetivo. Determinar las frecuencias génicas y de fenotipo, y predecir el riesgo de incompatibilidad y aloinmunización materna en la población de La Paz, Baja California Sur, México. Material y métodos. Estudio descriptivo en el que se evaluaron 1 809 disponentes sanguíneos altruistas que acudieron en 1998 al Hospital General de Zona, del Instituto Mexicano del Seguro Social en La Paz, Baja California Sur, México, tipificados por aglutinación en tubo. Las frecuencias génicas fueron estimadas asumiendo condiciones de equilibrio, y las incompatibilidades y riesgo de aloinmunización de acuerdo con la literatura. Los datos fueron evaluados estadísticamente mediante ji². Resultados. La población reveló las siguientes frecuencias en porcentaje: O, 58.49; A, 31.40; B, 8.40; AB, 1.71; RhD, 95.36; y RhD negativo, 4.64 y las frecuencias génicas fueron i, 0.7648; I A, 0.1821; I B, 0.0519; D, 0.7845; d, 0.2155, respectivamente. Las incompatibilidades en parejas y maternofetal fueron 0.3023 y 0.1685 para ABO, 0.0442 y 0.0364 para RhD y 0.0134 y 0.0061 para incompatibilidad doble, respectivamente. La probabilidad de aloinmunización materna fue estimada en 0.0309. Conclusiones. En La Paz, los grupos O y RhD son los más abundantes, aunque las frecuencias están entre las más bajas en México, contrario a lo ocurrido para A y RhD negativo. La probabilidad de aloinmunización materna y las incompatibilidades son también elevadas. Los grupos ancestrales blanco, negro e indio interactuaron en la zona noroeste del país, determinando al migrar a Baja California Sur un mestizaje probablemente similar al resto de la zona noroeste.Objective. To determine genic and phenotypic frequencies and predict the risk of incompatibility and maternal alloimmunization in the population of La Paz. Material and Methods. This descriptive study evaluated 1809 voluntary blood donors attending in 1998 the Hospital General de Zona of Instituto Mexicano del Seguro Social

  12. ABO Uygunsuzluğuna Bağlı Neonatal Hiperbilirubinemi

    Takcı, Şahin; İnce, Deniz Anuk; Hendekçi, Ayşe; Eren, Nagehan

    2014-01-01

    Aim: ABO incompatibility is a common condition occurring in about 15-25% of all maternal/fetal pairs. The features of ABO incompatibility range from asymptomatic through to severe hemolysis with hyperbilirubinemia and anemia. The aim of this study is to assess the clinical course of ABO incompatibility and to evaluate the effect of blood groups on the severity of neonatal jaundice. Material and Methods: Neonates with ABO hemolytic disease of newborn were retrospectively studied. Risk fact...

  13. ABO血型不合肝移植治疗急危重症肝病患者的临床疗效分析%Analysis of the curative effect of ABO-incompatible liver transplantation in the treatment in patients with acute severe liver disease

    沈中阳; 邓永林; 郑虹; 潘澄; 张雅敏; 蒋文涛; 张建军; 高伟; 淮明生

    2014-01-01

    Objective To analyze and evaluate the clinical effect of ABO-incompatible liver transplantation in the treatment of acute severe liver disease.Methods A retrospective clinical study was conducted.The clinical data of 4 136 patients undergoing orthotopic liver transplantation in Organ Transplantation Center of Tianjin First Center Hospital from September 1999 to December 2013 were analyzed.The criteria of patients enrolled were as following:model for end-stage liver disease (MELD) score ≥ 20,the donor's and recipient's blood types were different,age 18-70 years,and undergone primary non-bypass orthotopic liver transplantation.According to the rate of compliance with the principles of blood transfusion,the cases were divided into two groups:ABO-compatible group (ABO-C group,n =41),ABO-incompatible group (ABO-I group,n =22).The patients in ABO-I group received basiliximab + methylprednisolone for immune induction therapy during operation,basiliximab + tacrolimus + mycophenolate + cortisol as quadruple immunosuppressive regimen after operation.They also received subcutaneous injection of low molecular heparin for anticoagulant therapy after operation,and oral warfarin or aspirin and clopidogrel bisulfate instead after 7 days.They also received routine alprostadil after operation.The remaining treatment was the same as that of ABO-C group.The clinical data,postoperative complications,rejection and survival rates of two groups were statistically analyzed.Results There were no significant differences in gender,age,MELD score,complicated with tumor,quality of donor liver,length of cold preservation of donor liver,duration of operation,and blood loss during operation between ABO-C and ABO-I groups.Number of splenectomy during operation was significantly higher in ABO-I group than that in ABO-C group (5 cases vs.1 case,x2=4.687,P=0.030).The 3-month,6-month,1-year,3-year and 5-year survival rates of ABO-C group were 89.5%,78.3%,72.5%,69.1% and 61.8

  14. Intravenous Immunoglobulin G Treatment in ABO Hemolytic Disease of the Newborn, is it Myth or Real?

    Beken, Serdar; Hirfanoglu, Ibrahim; Turkyilmaz, Canan; Altuntas, Nilgun; Unal, Sezin; Turan, Ozden; Onal, Esra; Ergenekon, Ebru; Koc, Esin; Atalay, Yildiz

    2012-01-01

    Intravenous Immunoglobulin G (IVIG) therapy has been used as a component of the treatment of hemolytic disease of the newborn. There is still no consensus on its use in ABO hemolytic disease of the newborn routinely. The aim of this study is to determine whether administration of IVIG to newborns with ABO incompatibility is necessary. One hundred and seventeen patients with ABO hemolytic disease and positive Coombs test were enrolled into the study. The subjects were healthy except jaundice. ...

  15. Blood ties: ABO is a trans-species polymorphism in primates

    Ségurel, Laure; Thompson, Emma E.; Flutre, Timothée; Lovstad, Jessica; Venkat, Aarti; Margulis, Susan W.; Moyse, Jill; Ross, Steve; Gamble, Kathryn; Sella, Guy; Ober, Carole; Przeworski, Molly

    2012-01-01

    The ABO histo-blood group, the critical determinant of transfusion incompatibility, was the first genetic polymorphism discovered in humans. Remarkably, ABO antigens are also polymorphic in many other primates, with the same two amino acid changes responsible for A and B specificity in all species sequenced to date. Whether this recurrence of A and B antigens is the result of an ancient polymorphism maintained across species or due to numerous, more recent instances of convergent evolution ha...

  16. The ABO blood group is a trans-species polymorphism in primates

    Ségurel, Laure; Thompson, Emma E.; Flutre, Timothée; Lovstad, Jessica; Venkat, Aarti; Margulis, Susan W.; Moyse, Jill; Ross, Steve; Gamble, Kathryn; Sella, Guy; Ober, Carole; Przeworski, Molly

    2012-01-01

    The ABO histo-blood group, the critical determinant of transfusion incompatibility, was the first genetic polymorphism discovered in humans. Remarkably, ABO antigens are also polymorphic in many other primates, with the same two amino acid changes responsible for A and B specificity in all species sequenced to date. Whether this recurrence of A and B antigens is the result of an ancient polymorphism maintained across species or due to numerous, more recent instances of convergent evolution ha...

  17. Incompatible Images

    Sassene, Michel J.; Hertzum, Morten

    is, however, based on a taken-for-granted image of asthmatics as, per se, striving to be symptom-free. This image is incompatible with interviewed asthmatics' day-to-day performances of their asthma, and renders invisible (a) that their asthma performances emphasize an economy of good passages and of...

  18. Role of Subgroup Incompatibility in Newborn Jaundice Requiring Exchange Transfusion

    Annagür, Ali; Altunhan, Hüseyin; Konak, Murat; Koç, Hasan; Örs, Rahmi

    2014-01-01

    We aimed to determine the role of exchange transfusion related complications, treatment, and etiology as well as subgroup incompatibility in patients subject to ET (exchange transfusion) due to newborn jaundice. 82 patients hospitalized due to newborn jaundice and exposed to exchange transfusion between August 2007 and August 2011 were retrospectively studied. Before ET mean total serum bilirubin was 29,2±9,83. The most frequent cause of ET was ABO incompatibility (31%) followed by...

  19. Neonatal BO Incompatibility Is Associated With a Positive Cord Blood Direct Antiglobulin Test in Infants of Black Ethnicity.

    Özgönenel, Bülent; Kukreja, Geetika; O'Malley, Barbara; Bluth, Martin H

    2015-11-01

    ABO hemolytic disease of the newborn occurs almost exclusively in infants of blood group A and B who are born to group O mothers. Positive Direct Antiglobulin Test (DAT) can identify those infants who are at risk of developing the ABO hemolytic disease. Earlier studies have suggested that BO incompatibility is associated with a positive DAT in black infants. In this study we sought to determine whether ABO incompatibility type could be associated with a higher rate of DAT positivity or clinical hemolytic disease. We reviewed the electronic medical records of all ABO-incompatible births over a 2-year period. There were 1537 ABO-incompatible births during the study period. DAT was more commonly positive among BO incompatible (21.5% in BO vs. 14.8% in AO, P=0.001) and black (18.8% in blacks vs. 10.8% in nonblacks, P=0.003) infants. DAT positivity was significantly associated with both severe hyperbilirubinemia (P=0.028) and hemolytic anemia (P<0.001). BO incompatibility was significantly associated with hemolytic anemia, but not severe hyperbilirubinemia, in the infants tested. PMID:26422285

  20. Effect of different proportions of mixed blood exchange transfusion on blood circulation in neonates with ABO incompatibility hemolytic disease%不同比例成分血对新生儿母子ABO血型不合溶血病换血治疗后内环境影响研究

    李军; 黄瑞文; 胡月圆; 肖艾青; 廖镇宇

    2011-01-01

    Objective To explore the effect of different proportions (the O type RBCs vs the AB type plasma)of mixed blood exchange transfusion on blood circulation in neonates with ABO incompatibility hemolytic disease. Methods From Jan. 2009 to Apr. 2010 in Hunan Children's Hospital,forty newborn infants with the disease, treated by exchange transfusion with different proportions of mixed blood (AB type plasma was mixed with 0 type red blood cell), were randomly divided into two groups: 16 patients in treatment group, who used 0 type red blood cells and AB type plasma with the proportion of 3 : 1 ( the 0 type RBCs 300 mL: the AB type plasma 100 mL) during blood transfusion; 24 patients in control group with the proportion of 2: 1. Both of the two groups received the double exchange transfusion in accordance with 150 ~ 180 mL/kg. After exchange, all the patients in controll group received 0 type RBCs transfusion with 15 ~ 20 mlAg (total volume was 60mL), while the other group did not. The other therapies in both of the two groups were the same.The indicators, which included the exchange rate of neonatal serum bilirubin, RBC, hemoglobin (Hb), hematocrit (Hct) , the elctrocyte and gas analysis, were monitored before and after the exchange transfusion. Results The total bilirubin of the two groups were lower after the exchange transfusion, and that the treatment group was even lower(P 0.05), while in the control group the difference was significant (P 0.05). Conclusion The exchange transfusion, using 0 type red blood cells and AB type plasma with the proportion of 3 : 1 inhemolytic disease, can not only decrease the level of bilirubin in blood rapidly but also avoid the anemia.The serum electrolytes and acid-base balance have no signifi-cant differences. It is worth promoting.%目的 探讨不同比例成分血对新生儿母子ABO血型不合溶血病(HDN)换血治疗后内环境的影响.方法 对2009年1月至2010年4月湖南省儿童医院40例HDN达到换血指征的

  1. Maximally incompatible quantum observables

    The existence of maximally incompatible quantum observables in the sense of a minimal joint measurability region is investigated. Employing the universal quantum cloning device it is argued that only infinite dimensional quantum systems can accommodate maximal incompatibility. It is then shown that two of the most common pairs of complementary observables (position and momentum; number and phase) are maximally incompatible.

  2. Maximally incompatible quantum observables

    Heinosaari, Teiko, E-mail: teiko.heinosaari@utu.fi [Turku Centre for Quantum Physics, Department of Physics and Astronomy, University of Turku, FI-20014 Turku (Finland); Schultz, Jussi, E-mail: jussi.schultz@gmail.com [Dipartimento di Matematica, Politecnico di Milano, Piazza Leonardo da Vinci 32, I-20133 Milano (Italy); Toigo, Alessandro, E-mail: alessandro.toigo@polimi.it [Dipartimento di Matematica, Politecnico di Milano, Piazza Leonardo da Vinci 32, I-20133 Milano (Italy); Istituto Nazionale di Fisica Nucleare, Sezione di Milano, Via Celoria 16, I-20133 Milano (Italy); Ziman, Mario, E-mail: ziman@savba.sk [RCQI, Institute of Physics, Slovak Academy of Sciences, Dúbravská cesta 9, 84511 Bratislava (Slovakia); Faculty of Informatics, Masaryk University, Botanická 68a, 60200 Brno (Czech Republic)

    2014-05-01

    The existence of maximally incompatible quantum observables in the sense of a minimal joint measurability region is investigated. Employing the universal quantum cloning device it is argued that only infinite dimensional quantum systems can accommodate maximal incompatibility. It is then shown that two of the most common pairs of complementary observables (position and momentum; number and phase) are maximally incompatible.

  3. ABO blood groups, rhesus factor and pemphigus

    Valikhani Mahin; Kavand Sima; Toosi Siavash; Kavand Golnaz; Ghiasi Maryam

    2007-01-01

    Background: Pemphigus is an autoimmune blistering disease of the skin and mucous membranes with significant mortality and morbidity. Genetic factors are known to be involved in pemphigus. Several studies have reproducibly shown significant associations of ABO blood groups with various autoimmune human diseases. Aim: To study the relationship between ABO and Rhesus (D) blood groups and pemphigus in Iranian patients. Materials and Methods: Data on age, sex, ABO and Rhesus blood type and cl...

  4. Incompatibility and Mental Fatigue

    Herzog, Thomas R.; Hayes, Lauren J.; Applin, Rebecca C.; Weatherly, Anna M.

    2011-01-01

    A straightforward prediction from attention restoration theory is that the level of incompatibility in a person's life should be positively correlated with that person's level of mental (or directed attention) fatigue. The authors tested this prediction by developing a new self-report measure of incompatibility in which they attempted to isolate…

  5. Incompatibility breaking quantum channels

    A typical bipartite quantum protocol, such as EPR-steering, relies on two quantum features, entanglement of states and incompatibility of measurements. Noise can delete both of these quantum features. In this work we study the behavior of incompatibility under noisy quantum channels. The starting point for our investigation is the observation that compatible measurements cannot become incompatible by the action of any channel. We focus our attention to channels which completely destroy the incompatibility of various relevant sets of measurements. We call such channels incompatibility breaking, in analogy to the concept of entanglement breaking channels. This notion is relevant especially for the understanding of noise-robustness of the local measurement resources for steering. (paper)

  6. ABO blood groups, rhesus factor and pemphigus

    Valikhani Mahin

    2007-01-01

    Full Text Available Background: Pemphigus is an autoimmune blistering disease of the skin and mucous membranes with significant mortality and morbidity. Genetic factors are known to be involved in pemphigus. Several studies have reproducibly shown significant associations of ABO blood groups with various autoimmune human diseases. Aim: To study the relationship between ABO and Rhesus (D blood groups and pemphigus in Iranian patients. Materials and Methods: Data on age, sex, ABO and Rhesus blood type and clinicopathological diagnosis of the patients with pemphigus were collected. A total of 573 patients with pemphigus were assessed for their association with ABO or Rhesus (D blood groups and compared with the normal population in the area. Results: The distribution of ABO and Rhesus blood groups in patients with pemphigus was similar to the normal local population in Iran. No relationship was found between ABO or Rhesus blood groups and the phenotype of pemphigus. Conclusion: It appears that there is no association between ABO or Rhesus (D blood groups and the frequency of pemphigus variants in comparison with normal population in Iran.

  7. PERCENTAGE OF SANGUIN GROUP (ABO, RH

    M.Mirdamadi

    1978-09-01

    Full Text Available The ABO and Rh Blood groups of 9753 pregnant women were determined in the Amin Maternity Hospital, Isfahan, Iran, from March 23, 1976 to July 11, 1977. Their ABO blood group frequencies were calculated as follows: 0=38.20%, A=30.67%, B=23.84% and AB=7.26%. Also, 90.02% of the women were Rh positive and the rest, namely, 9.98% were Rh negative.

  8. Enhanced agglutination reaction of ABO subgroups by gold nanoparticle solution: implication for identification of ABO subgroups.

    Ammaranond, P; Sriyarak, J; Saejong, S; Deesin, P; Seereemaspun, A; Rojanathanes, R

    2011-12-01

    Although the ABO blood group is the most significant in blood group system in human, other subgroups system is also important to be concerned in blood banking laboratory. ABO subgroups have weak antigen potency on red blood cell. In some cases, they could not been detected by cell grouping and serum grouping methods. This may lead to misinterpretation of ABO typing which will cause serious problems for transfusion and transplantation. Gold nanoparticle solution can increase the agglutination reaction of ABO typing. Thus far, the investigation of ABO blood group system has been performed using gold nanoparticle solution. Samples were tested comparing between with and without gold nanoparticle solution. After reading the agglutination reaction, supernatants were collected and measured at the optical density at 760 nm by spectrophotometer. The optical density of 2-5% cell suspension and monoclonal antibody was higher than in the tube of 2-5% cell suspension, monoclonal antibody and gold nanoparticle solution. By adding the gold nanoparticle solution, the agglutination reaction was increased ranging from 7.0-37.7% (median 15.0%) for ABO grouping system whereas 12.1-50.9% (median 23.4%) was observed in ABO subgroups. It could decrease the chance of misinterpretation by 33.3%. By using gold nanoparticle solution might be the alternative way for investigation of weak antigen potency on red blood cell. PMID:22416584

  9. ABO blood group and risk of cancer

    Vasan, Senthil K; Hwang, Jinseub; Rostgaard, Klaus;

    2016-01-01

    INTRODUCTION: The associations between ABO blood group and cancer risk have been studied repeatedly, but results have been variable. Consistent associations have only been reported for pancreatic and gastric cancers. MATERIALS AND METHODS: We estimated associations between different ABO blood...... groups and site-specific cancer risk in a large cohort of healthy blood donors from Sweden and Denmark. RESULTS: A total of 1.6 million donors were followed over 27 million person-years (20 million in Sweden and 7 million in Denmark). We observed 119,584 cancer cases. Blood groups A, AB and B were...... associated either with increased or decreased risk of cancer at 13 anatomical sites (p≤0.05), compared to blood group O. Consistent with assessment using a false discovery rate approach, significant associations with ABO blood group were observed for cancer of the pancreas, breast, and upper gastrointestinal...

  10. Plates with Incompatible Prestrain

    Bhattacharya, Kaushik; Lewicka, Marta; Schäffner, Mathias

    2016-01-01

    We study effective elastic behavior of the incompatibly prestrained thin plates, where the prestrain is independent of thickness and uniform through the plate’s thickness h. We model such plates as three-dimensional elastic bodies with a prescribed pointwise stress-free state characterized by a Riemannian metric G, and seek the limiting behavior as h→0. We first establish that when the energy per volume scales as the second power of h, the resulting Γ-limit is a Kirchhoff-type bending theory....

  11. ABO research in the modern era of genomics.

    Yamamoto, Fumiichiro; Cid, Emili; Yamamoto, Miyako; Blancher, Antoine

    2012-04-01

    Research on ABO has advanced significantly in recent years. A database was established to manage the sequence information of an increasing number of novel alleles. Genome sequencings have identified ABO orthologues and paralogues in various organisms and enhanced the knowledge on the evolution of the ABO and related genes. The most prominent advancements include clarification of the association between ABO and different disease processes. For instance, ABO status affects the infectivity of certain strains of Helicobacter pylori and Noroviruses as well as the sequestration and rosetting of red blood cells infected with Plasmodium falciparum. Genome-wide association studies have conclusively linked the ABO locus to pancreatic cancer, venous thromboembolism, and myocardial infarction in the presence of coronary atherosclerosis. These findings suggest ABO's important role in determining an individual's susceptibility to such diseases. Furthermore, our understanding of the structures of A and B transferases and their enzymology has been dramatically improved. ABO has also become a research subject in neurobiology and the preparation of artificial/universal blood and became a topic in the pseudoscience of "blood type diets." With such new progress, it has become evident that ABO is a critical player in the modern era of genomic medicine. This article provides the most up-to-date information regarding ABO genomics. PMID:21945157

  12. [Mistyping of ABO grouping by polyagglutination].

    Kato, T; Ishimaru, K; Sekiguchi, S

    1997-09-01

    Polyagglutination is a phenomenon that a sample of patient's red blood cells is agglutinated by most of normal human sera. In patients with bacterial infection or hematological diseases, red cells may become agglutinable due to an exposure of antigens (cryptoantigen) that are usually hidden as submembrane structures of normal red cells. Most of normal sera contain natural antibodies to the corresponding antigens. Polyagglutination can cause apparent discrepancies between ABO antigen and antibody tests with the patient's sample, resulting in mistyping of ABO grouping. In some cases, polyagglutination is observed only by the minor test of cross-matching without showing the discrepancies. Recently polyagglutination has been rarely seen probably because of increasing use of monoclonal antibodies and dispensing minor test for cross-matching. Because no obvious case of adverse reaction by polyagglutination through transfusion is recently reported, clinical significance of polyagglutination seems extremely low. PMID:9301304

  13. [A population-genetics approach to the problem of nonspecific biological resistance of the human body. III. The ABO and rhesus blood group systems of healthy and sick children and their mothers].

    Kurbatova, O L; Botvin'ev, O K; Altukhov, Iu P

    1984-04-01

    ABO and Rhesus blood types have been specified in 2047 diseased newborns, diseased infants and children who died before the age of one, as well as in their mothers. 527 healthy children and their mothers were investigated as a control group. A significant difference in the ABO phenotype frequencies has been revealed between: i) healthy and dead children, ii) mothers of diseased newborns and mothers of healthy children, iii) dead children and their mothers. The significant increase in the incidence of maternal Rhesus-negative phenotype, as compared with the control group, was shown in the groups of diseased newborns, diseased infants and dead children. In the same groups, mothers differ significantly from their children with respect to the frequency of Rhesus phenotypes. The incidence of Rhesus-incompatible mother-child pairs in the groups of diseased newborns, diseased infants and dead children was shown to be two times higher than the respective frequency in the control group and the expected frequency. A certain increase in the frequency of ABO-incompatible pairs was revealed in the groups of diseased newborns and dead children, but the difference, as compared to the control group, did not prove to be statistically significant. A hypothesis was advanced to the effect that the mother-child incompatibility for Rhesus and ABO antigens may result not only in fetal wastage and haemolytic disease of newborns, but also in the decrease of child's resistance to diseases of different origin. PMID:6427064

  14. Plates with Incompatible Prestrain

    Bhattacharya, Kaushik; Lewicka, Marta; Schäffner, Mathias

    2016-07-01

    We study effective elastic behavior of the incompatibly prestrained thin plates, where the prestrain is independent of thickness and uniform through the plate's thickness h. We model such plates as three-dimensional elastic bodies with a prescribed pointwise stress-free state characterized by a Riemannian metric G, and seek the limiting behavior as {h to 0}. We first establish that when the energy per volume scales as the second power of h, the resulting {Γ} -limit is a Kirchhoff-type bending theory. We then show the somewhat surprising result that there exist non-immersible metrics G for whom the infimum energy (per volume) scales smaller than h 2. This implies that the minimizing sequence of deformations carries nontrivial residual three-dimensional energy but it has zero bending energy as seen from the limit Kirchhoff theory perspective. Another implication is that other asymptotic scenarios are valid in appropriate smaller scaling regimes of energy. We characterize the metrics G with the above property, showing that the zero bending energy in the Kirchhoff limit occurs if and only if the Riemann curvatures R 1213, R 1223 and R 1212 of G vanish identically. We illustrate our findings with examples; of particular interest is an example where {G_{2 × 2}}, the two-dimensional restriction of G, is flat but the plate still exhibits the energy scaling of the Föppl-von Kármán type. Finally, we apply these results to a model of nematic glass, including a characterization of the condition when the metric is immersible, for {G = Id3 + γ n ⊗ n} given in terms of the inhomogeneous unit director field distribution { n in R^3}.

  15. Naturally occurring anti M complicating ABO grouping

    Safoorah Khalid

    2011-01-01

    Full Text Available Anti M is considered a naturally occurring antibody that is usually active at temperatures below 37°C and is thus of no clinical significance. This antibody, if present in an individual, can lead to a discrepancy between forward and reverse ABO grouping and thus creates diagnostic difficulties for blood bank staff. We report a case of a 58-year-old lady who had an unexpected reaction in reverse grouping due to anti M that posed a problem for us in the interpretation of results of her blood group. We also reviewed the literature to find out the significance of such discrepancy in blood grouping.

  16. Naturally occurring anti M complicating ABO grouping.

    Khalid, Safoorah; Dantes, Roelyn; Varghese, Sunu; Al Hakawati, Imadeddin

    2011-01-01

    Anti M is considered a naturally occurring antibody that is usually active at temperatures below 37°C and is thus of no clinical significance. This antibody, if present in an individual, can lead to a discrepancy between forward and reverse ABO grouping and thus creates diagnostic difficulties for blood bank staff. We report a case of a 58-year-old lady who had an unexpected reaction in reverse grouping due to anti M that posed a problem for us in the interpretation of results of her blood group. We also reviewed the literature to find out the significance of such discrepancy in blood grouping. PMID:21393909

  17. Naturally occurring anti M complicating ABO grouping

    Safoorah Khalid; Roelyn Dantes; Sunu Varghese; Imadeddin Al Hakawati

    2011-01-01

    Anti M is considered a naturally occurring antibody that is usually active at temperatures below 37°C and is thus of no clinical significance. This antibody, if present in an individual, can lead to a discrepancy between forward and reverse ABO grouping and thus creates diagnostic difficulties for blood bank staff. We report a case of a 58-year-old lady who had an unexpected reaction in reverse grouping due to anti M that posed a problem for us in the interpretation of results of her blood gr...

  18. A Review of ABO3 Perovskite Photocatalysts for Water Splitting

    Zhang Hongjie; Chen Gang; Li Zhonghua; Liu Jiangwen

    2007-01-01

    Photocatalysts with perovskites for hydrogen production from aqueous solution were reviewed. Among the most of metal oxide photocatalysts, the family of ABO3 Perovskite-type oxide shows higher photocatalytie activity, especially alkaline earth titanate and alkali tantalate. Therein, sodium tantalate showed the highest activity for water splitting. The reasons for the high photocatalytic activity of ABO3 perovskties are considered to the diverse and flexible crystal structure. The photocatalytic activity of ABO3 perovskties can be improved by doping other element at A site, B site or O site and loading CO-catalysts such as NiO and Pt. In this paper, the mechanism of photocatalytic water splitting, the structure of ABO3 perovsktie, and Perovskite-type photocatalysts were reviewed.

  19. ABO blood group and breast cancer incidence and survival

    Gates, Margaret A.; Xu, Mousheng; Chen, Wendy Y.; Kraft, Peter; Hankinson, Susan E; Wolpin, Brian M.

    2012-01-01

    ABO blood type has been associated with risk and survival for several malignancies; however, data for an association with breast cancer are inconsistent. Our study population consisted of Nurses’ Health Study participants with self-reported serologic blood type and/or ABO genotype. Using Cox proportional hazards regression, we examined the association between serologic blood type and incident breast cancer among 67,697 women, including 3,107 cases. In addition, we examined the association wit...

  20. Significant association between ABO blood group and pancreatic cancer

    Julia; B; Greer; Mark; H; Yazer; Jay; S; Raval; M; Michael; Barmada; Randall; E; Brand; David; C; Whitcomb

    2010-01-01

    AIM:To evaluate whether the ABO blood group is related to pancreatic cancer risk in the general population of the United States.METHODS:Using the University of Pittsburgh's clinicalpancreatic cancer registry,the blood donor database from our local blood bank (Central Blood Bank),and the blood product recipient database from the regional transfusion service (Centralized Transfusion Service) in Pittsburgh,Pennsylvania,we identified 274 pancreatic cancer patients with previously determined serological ABO bloo...

  1. Results of exchange transfusions in newborns without blood group incompatibility

    Servet Yel

    2013-01-01

    Full Text Available Objective: Hyperbilirubinemia is a common problem ofneonatal period that has high morbidity and mortality.Blood exchange is the most effective and urgent treatmentmodality for very high bilirubin levels that can lead toneurotoxicity called as kernicterus. The aim of this studywas to compare 90 minutes exchange transfusion withthat of 120 minutes.Methods: This study was performed at Dicle UniversityMedical Faculty, Neonatal Unit between July 2007 andJune 2008. A total of 36 term newborn (38 - 42 gestationalweek without blood group incompatibility and withtotal serum bilirubin levels over 25 mg/dl were included.Newborns were randomly assigned in two groups eachof them comprise 18 babies as Group 1 underwent 90minute-exchange and Group 2 120 minute. Effectivenessand complications of exchange transfusion were recorded.Newborns with Rh, ABO or subgroup incompatibilities,prematurity or small for gestational age, septicemia,hypothyroidism, G6PD enzyme deficiency, intrauterineinfections, diabetic mother’s baby, hemolytic disease ormetabolic diseases were excluded.Results: There were no significant differences in thebody weight, gestational age, postnatal age, age of mother,total bilirubin and albumin levels, the number of bloodexchange, hospital stay days and complications betweentwo groups (p>0.05. However, mean phototherapy durationwas significantly shorter in 120 minutes transfusiongroup compared with 90 minutes group (p<0.001.Conclusion: Our results indicated that 90 minutes wassufficient for an effective exchange transfusion in severehyperbilirubinemic newborn infants. However longer exchangetransfusion durations may shorten the duration ofphototherapy.Key words: Indirect hyperbilirubinemia, exchange transfusion,newborns, outcome

  2. Bell inequalities and incompatible measurements

    Morgan, Peter

    2003-01-01

    Bell inequalities are a consequence of measurement incompatibility (not, as generally thought, of nonlocality). In classical terms, this is equivalent to contextuality -- measurement devices do have a significant effect. Contextual models are reasonable in classical physics, which always took the view that we ignore measurement devices whenever possible, but if that isn't good enough then we do have to model measurement devices. It is also argued that quantum theory should only be taken with ...

  3. ABO-Nonidentical Liver Transplantation in the United States.

    Lai, J C; Roberts, J P

    2016-08-01

    Under the United Network for Organ Sharing (UNOS) policy, deceased donor livers may be offered to ABO-nonidentical candidates at each given Model for End-Stage Liver Disease (MELD) score and to blood type B candidates at MELD ≥30. To evaluate ABO-nonidentical liver transplantation (LT) in the United States, we examined all adult LT non-status 1 candidates, recipients and deceased liver donors from 2013 to 2015. There were 34 920 LT candidates (47% type O, 38% type A, 12% type B, 3% type AB) and 10 479 deceased liver donors (47% type O, 38% type A, 12% type B, 3% type AB). ABO-nonidentical LT occurred in 2%, 3%, 20% and 36% of types O, A, B and AB recipients, respectively, which led to a net liver loss of 6% for type O and 2% for type A recipients but a net liver gain of 14% for type B and 55% for type AB recipients. The LT MELD scores of ABO-identical versus -nonidentical recipients were 29 versus 34 for type O, 29 versus 19 for type A, 25 versus 38 for type B, and 22 versus 28 for type AB (p liver supply for candidates with blood types B and AB but decreased supply for type O and A candidates. We urge refinement of UNOS policy surrounding ABO-nonidentical LT. PMID:26932134

  4. ABO Blood Group System and Gastric Cancer: A Case-Control Study and Meta-Analysis

    Yingyan Yu; Zhenggang Zhu; Jun Zhang; Min Yan; Bingya Liu; Jianian Zhang; Jun Ji; Zhiwei Wang; Lei Liu

    2012-01-01

    This study focuses on the association between the ABO blood group system and the risk of gastric cancer or Helicobacter pylori infection. The data for the ABO blood group was collected from 1045 cases of gastric cancer, whereby the patient underwent a gastrectomy in Ruijin Hospital, Shanghai. The information on the ABO blood group from 53,026 healthy blood donors was enrolled as control. We searched the Pubmed database on the relationship between ABO blood groups and gastric cancer risk for m...

  5. On Algebraic properties of ABO-Blood Type Inheritance Pattern

    Casas, J. M.; Ladra, M.; Omirov, B. A.; Turdibaev, R.

    2014-01-01

    We generate an algebra on blood phenotypes with multiplication based on human ABO-blood type inheritance pattern. We assume that during meiosis gametes are not chosen randomly. For this algebra we investigate its algebraic properties. Namely, the lattice of ideals and the associative enveloping algebra are described.

  6. Primate ABO Gene is under Weak Positive Selection

    Eliane Santos EVANOVICH

    2012-05-01

    Full Text Available ABO locus presents three main alleles: A, B and O. A and B encode glycosyltransferases that catalyze the addiction of an N-GalNac and D-galactose to a precursor substance (H substance, producing A and B antigens, while the O allele does not produce a functional protein. The presence of A and B antigens have been associated to resistance against infectious agents which could use them as attachment factors increasing the virulence of some parasitic agents. As these antigens are not restrict to humans, analyses them in others species, for instance non-human primates, may be crucial to understand the relationship between pathogens and ABO phenotypes. Despite of the relevance of this issue, in the last decade few studies have addressed, mainly in New World Monkeys (NWM, natural reservoir of tropical diseases in Amazon Region. In order to understand the evolution of the ABO system in the primates, it has been obtained the partial sequence of the most important exon of ABO gene (exon 7, in platyrrhini families: Atelidae, Pithecidae and Cebidae. Then, it has been compared the sequences obtained those present in the literature, and measured the selective pressure. The present results shown that residues 266 and 268 are also crucial to distinguish A and B phenotypes in the platyrrhines, such as in catarrhines, and the 266 codon is under positive selection, although the most site codons are under action of purifying selection.

  7. [ABO BLOOD GROUPS AS RISK FACTOR IN HELICOBACTER PYLORI INFECTION

    Gonzáles Flores, Pedro Alejandro; Díaz Ferrer, Javier Omar; Monge Salgado, Eduardo; Watanabe Varas T, Teresa

    2000-01-01

    TITLE: ABO blood groups as risk factor in Helicobacter pylori infection.OBJECTIVE: To asses the relation between ABO blood groups and Helicobacter pylori (Hp) infection. METHODS: The present is a case and control study. A study population of dyspeptic patients who underwent upper gastrointestinal endoscopy was selected. Four biopsies were taken from the antrum and the body of the stomach and blood group was typified. Patients with gastrectomy, gastric cancer, treated for Hp infection in the previous six months or without blood group typification were excluded. The population sample was found using EPIINFO 5.1 program. We called case to every patient with Hp (+) biopsy and control all with Hp (-) biopsy. The risk of the infection was calculated with the OR (Odds ratio) and the study sample was compared with the blood bank control group using the Chi-square test (pblood groups between the study population and the blood bank control. When we compared the ABO blood distribution between patients Hp (+) and Hp (-) we found significant differences for blood group O (p=0.004) and blood group A (p=0.03). Statistical analysis revealed an OR=2,22 for the blood group O and OR=0,5 for the blood group A.CONCLUSIONS: 1) The ABO blood group distribution is different in patients with Hp infection compared with those without Hp infection. 2) Blood group O would be a moderate risk factor for infection by Helicobacter pylori. PMID:12140571

  8. Genetic characterization of the ABO blood group in Neandertals

    Bertranpetit Jaume

    2008-12-01

    Full Text Available Abstract Background The high polymorphism rate in the human ABO blood group gene seems to be related to susceptibility to different pathogens. It has been estimated that all genetic variation underlying the human ABO alleles appeared along the human lineage, after the divergence from the chimpanzee lineage. A paleogenetic analysis of the ABO blood group gene in Neandertals allows us to directly test for the presence of the ABO alleles in these extinct humans. Results We have analysed two male Neandertals that were retrieved under controlled conditions at the El Sidron site in Asturias (Spain and that appeared to be almost free of modern human DNA contamination. We find a human specific diagnostic deletion for blood group O (O01 haplotype in both Neandertal individuals. Conclusion These results suggest that the genetic change responsible for the O blood group in humans predates the human and Neandertal divergence. A potential selective event associated with the emergence of the O allele may have therefore occurred after humans separated from their common ancestor with chimpanzees and before the human-Neandertal population divergence.

  9. Immobilization to prevent enzyme incompatibility with proteases

    Vossenberg, P.; Beeftink, H.H.; Cohen Stuart, M.A.; Tramper, J.

    2011-01-01

    Enzyme incompatibility is a problem in multi-enzyme processes that involve a non-specific protease, such as Alcalase. An example is the one-pot enzymatic synthesis of peptides catalyzed by a lipase and a protease. The incompatibility between lipase B from Candida antarctica (CalB) and Alcalase was s

  10. Delayed hyperacute rejection in a patient who developed clostridium difficile infection after ABO-incompatible kidney transplantation

    Lipshutz, Gerald S.; Reed, Elaine F; Phuong-Chi Pham; et al.

    2010-01-01

    Gerald S Lipshutz1, Elaine F Reed2, Phuong-Chi Pham3, Jeffrey M Miller4, Jennifer S Singer5, Gabriel M Danovitch6, Alan H Wilkinson6, Dean W Wallace7, Suzanne McGuire6, Phuong-Truc Pham8, Phuong-Thu Pham61Department of Surgery, Kidney and Pancreas Transplant Program, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA; 2Department of Pathology and Laboratory Medicine-Immunogenetics, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA; 3Department of Medicine, Nephrology Di...

  11. Robustness of incompatibility for quantum devices

    A robustness measure for incompatibility of quantum devices in the lines of the robustness of entanglement is proposed. The concept of general robustness measures is first introduced in general convex-geometric settings and these ideas are then applied to measure how incompatible a given pair of quantum devices is. The robustness of quantum incompatibility is calculated in three special cases: a pair of Fourier-coupled rank-1 sharp observables, a pair of decodable channels, where decodability means left-invertibility by a channel, and a pair consisting of a rank-1 sharp observable and a decodable channel. (paper)

  12. Ready-made allogeneic ABO-specific serum eye drops

    Harritshøj, Lene Holm; Nielsen, Connie; Ullum, Henrik;

    2014-01-01

    severe ocular surface disorders. METHODS: Serum was derived from 450 ml whole-blood donations from regular male blood donors, produced and tested according to good manufacturing practice and legislation regulating blood products in Denmark. Serum was diluted to 20% (v/v) with NaCl 0.9%, filtered, bottled......PURPOSE: To overcome problems and delays of the preparation of autologous serum eye drops, a production line of ABO-specific allogeneic serum eye drops from male blood donors was set up in a blood bank. Feasibility, clinical routine, safety and efficacy were evaluated in a cohort of patients with......, registered and stored at -30°C in the blood bank. Upon request, frozen ABO-identical serum drops in lots of 14 bottles could be provided immediately. Safety and efficacy were evaluated in 34 patients with severe ocular surface disease refractory to conventional medical therapy. Patients were treated six...

  13. Structural Insights into Polymorphic ABO Glycan Binding by Helicobacter pylori.

    Moonens, Kristof; Gideonsson, Pär; Subedi, Suresh; Bugaytsova, Jeanna; Romaõ, Ema; Mendez, Melissa; Nordén, Jenny; Fallah, Mahsa; Rakhimova, Lena; Shevtsova, Anna; Lahmann, Martina; Castaldo, Gaetano; Brännström, Kristoffer; Coppens, Fanny; Lo, Alvin W; Ny, Tor; Solnick, Jay V; Vandenbussche, Guy; Oscarson, Stefan; Hammarström, Lennart; Arnqvist, Anna; Berg, Douglas E; Muyldermans, Serge; Borén, Thomas; Remaut, Han

    2016-01-13

    The Helicobacter pylori adhesin BabA binds mucosal ABO/Le(b) blood group (bg) carbohydrates. BabA facilitates bacterial attachment to gastric surfaces, increasing strain virulence and forming a recognized risk factor for peptic ulcers and gastric cancer. High sequence variation causes BabA functional diversity, but the underlying structural-molecular determinants are unknown. We generated X-ray structures of representative BabA isoforms that reveal a polymorphic, three-pronged Le(b) binding site. Two diversity loops, DL1 and DL2, provide adaptive control to binding affinity, notably ABO versus O bg preference. H. pylori strains can switch bg preference with single DL1 amino acid substitutions, and can coexpress functionally divergent BabA isoforms. The anchor point for receptor binding is the embrace of an ABO fucose residue by a disulfide-clasped loop, which is inactivated by reduction. Treatment with the redox-active pharmaceutic N-acetylcysteine lowers gastric mucosal neutrophil infiltration in H. pylori-infected Le(b)-expressing mice, providing perspectives on possible H. pylori eradication therapies. PMID:26764597

  14. Distribution of ABO blood group in children with acute leukemias

    Meliha Sakić

    2012-12-01

    Full Text Available Introduction: This study is the fi rst study about the distribution ABO blood types at children with acute leukemia in Federation of Bosnia and Herzegovina. The aim of the study is to point out distribution of blood type groups at children with acute leukemia (ALMethods: The number of children in this study was the following: 145 children with acute leukemia and 27 of children with acute myeloblastic leukemia (AML. All of the children were treated at Hemato- Oncology Unitof Pediatric Clinic in Sarajevo, in the period January 2000 until December 2010. Age of children was between 1 month and 15 years.Results: The results showed that different blood types were registered in 93. 1% of children who got ill and treated from acute leukemia for the mentioned period. At 6. 9 % of children, none of the blood types wereregistered. It was noticed that 40.9 % children who have registered blood type O, 37% blood type A,16% blood type B and 6.5% blood type AB had AL, too. It has been observed that children with following bloodtypes had AML: O, 47.8%, A, 47.7% and AB, 30.4%.Conclusion: Signifi cance ABO types distribution was confi rmed for children with ALL, p<0, 05. The analysis of the distribution of ABO types based on gender showed that signifi cance was confi rmed at females with both ALL and AML (p<0.05.

  15. Is ABO blood group truly a risk factor for thrombosis and adverse outcomes?

    Zhou, Shan; Welsby, Ian

    2014-01-01

    ABO blood type is one of the most readily available laboratory tests, and serves as a vital determinant in blood transfusion and organ transplantation. The ABO antigens are expressed not only on red blood cell membranes, determining the compatibility of transfusion, but also on the surface of other human cells, including epithelium, platelet and vascular endothelium, therefore extending the research into other involvements of cardiovascular disease and postoperative outcomes. ABO blood group ...

  16. Prognostic value of ABO blood group in patients with surgically resected colon cancer

    X. Cao; Wen, Z-S; Sun, Y-J; Li, Y.; Zhang, L.; Han, Y-J

    2014-01-01

    Background: Previous studies supported a link between the ABO blood type and survival for several types of malignancies. Nonetheless, the relationship between ABO blood type and survival in colon cancer patients has not been rigorously evaluated. The goal of this retrospective analysis was to discern the correlations between ABO blood group and colon cancer survival. Methods: A total of 1555 colon cancer patients that underwent curative-intent surgery between October 1995 and June 2002 were e...

  17. Role of ABO Secretor Status in Mucosal Innate Immunity and H. pylori Infection

    Sara Lindén; Jafar Mahdavi; Cristina Semino-Mora; Cara Olsen; Ingemar Carlstedt; Thomas Borén; Andre Dubois

    2008-01-01

    Author Summary The common ABO blood group antigen system was described in the early 20th century. In addition, it has been known for 60 years that the majority of individuals also express the corresponding ABO antigens (carbohydrate identity tags) in their saliva, tears, milk, and mucus secretions in the digestive tract. To this date, however, the biological function of the ABO blood group antigens has remained an enigma. Here, we show that the great majority of Rhesus monkeys are of blood gr...

  18. Prevalence of Phenotypes and Genes of ABO and Rhesus (Rh) Blood Groups in Faisalabad, Pakistan

    Amjad Hameed; Wajahat Hussain; Janbaz Ahmed; Fazli-Rabbi; Javed Anver Qureshi

    2002-01-01

    The frequencies of ABO and Rhesus (D) blood groups and influence of gender were determined on prevalence of these blood groups. Blood group B was found to be the most frequent (38.004%). The frequency of blood group O was 28.755%, for group A 23.260% and for group AB 9.981%. In Rhesus blood grouping system the frequency of Rhesus (D) positive was 89.094%. Gene frequencies with respect to ABO and Rhesus (D) negative were different as for only ABO and ABO along with Rhesus (D) positive. The tre...

  19. Molecular genotyping of ABO blood groups in some population groups from India

    Sabita Ray

    2014-01-01

    Full Text Available Background & objectives: Indian population is characterized by the presence of various castes and tribal groups. Various genetic polymorphisms have been used to differentiate among these groups. Amongst these, the ABO blood group system has been extensively studied. There is no information on molecular genotyping of ABO blood groups from India. Therefore, the main objective of this study was to characterize the common A, B and O alleles by molecular analysis in some Indian population groups. Methods: One hundred samples from the mixed population from Mumbai, 101 samples from the Dhodia tribe and 100 samples from the Parsi community were included in this study. Initially, the samples were phenotyped by standard serologic techniques. PCR followed by single strand conformational polymorphsim (SSCP was used for molecular ABO genotyping. Samples showing atypical SSCP patterns were further analysed by DNA sequencing to characterize rare alleles. Results: Seven common ABO alleles with 19 different genotypes were found in the mixed population. The Dhodias showed 12 different ABO genotypes and the Parsis revealed 15 different ABO genotypes with six common ABO alleles identified in each of them. Two rare alleles were also identified. Interpretation & conclusions: This study reports the distribution of molecular genotypes of ABO alleles among some population groups from India. Considering the extremely heterogeneous nature of the Indian population, in terms of various genotype markers like blood groups, red cell enzymes, etc., many more ABO alleles are likely to be encountered.

  20. Personality traits of aggression-submissiveness and perfectionism associate with ABO blood groups through catecholamine activities.

    Hobgood, Donna K

    2011-08-01

    Personality trait research has shown associations with many genes, prominently those of the catecholamine metabolism such as dopamine beta hydroxylase (DBH), catechol-O-methyltransferase (COMT), and monoamine oxidase A (MAOA). Because DBH gene is in linkage disequilibrium with ABO gene, there is reason to think that other catecholamine genes using the same substrate as DBH may also have associations with ABO blood groups, and this paper demonstrates how this may be so. Reasons include similarities in hapmap population frequency distributions, similarities in illness risks between ABO blood groups and DBH activities as well as between ABO blood groups and COMT activities and between ABO blood groups and MAOA activities. If ABO blood groups can be demonstrated to associate with all these catecholamine genes, then the catecholamine personality trait research can be applied to ABO blood groups and tested for confirmation. ABO blood typing is widely available and affords ability to test this hypothesis and thus confirm the possible joint association of personality traits of aggression-submissiveness and perfectionism to catecholamine genes and to ABO blood groups. Clinical applications and implications are discussed. PMID:21601990

  1. 足月新生儿 ABO 溶血病伴高胆红素血症的临床分析%Clinical analysis of neonatal ABO hemolytic disease with hyperbilirubinemia in full-term infants

    韩雪芹; 唐建军; 夏斌

    2016-01-01

    Objective To analyze characteristics of clinical manifestations,laboratory test results,therapeutic measurements and early prognosis of neonatal ABO incompatibility hemolytic disease (simply as "ABO hemolytic disease")with hyperbilirubinemia in full-term infants,and provid directions of reasonable management of this disease.Methods A total of 331 cases of full-term infants who were confirmed neonatal ABO hemolytic disease with hyperbilirubinemia by laboratory tests,and hospitalized in department of neonatology,West China Second University Hospital,Sichuan University from January 2012 to December 2014 were chosen as study objects.Clinical manifestations,laboratory test results,therapeutic measurements and early prognosis of medical history of these 331 cases were analyzed,retrospectively.The study protocol was approved by the Ethical Review Board of Investigation in Human Being of West China Second University Hospital,Sichuan University.Informed consent was obtained from the parents of each participant.Results The admission mean age was (53.5± 36.2 ) h of these 331 cases of full-term neonatal ABO hemolytic disease with hyperbilirubinemia,48.0%(1 59/331)patients were boys,45.0%(149/331 )patients were born from the first pregnancy,59.2%(1 96/331)patients were cesarean delivery,71.6%(237/331)patients were mixed feeding,the mean jaundice noticed time was (29.7 ± 1 9.4)h after born.The admission mean total serum bilirubin (TSB)was (233.1 ± 90.9)μmol/L,the TSB concentration of 9.4% (31/331 ) patients were>342.0 μmol/L,the highest was 614.7 μmol/L,14.5%(48/331)patients were admitted to hospital with anemia,56.8%(188/331 )patients with type A blood,the rate of eluate positive for anti-A/B test was higher than that of direct antiglobulin test(DAT)(95.5% vs 29.0%),and the difference was statistically significant(χ2 =31 1.076,P =0.000).Mean phototherapy time was (80.1± 20.9)h,8.8%(29/331)patients accepted blood exchange-transfusions and blood transfusions,1 9

  2. The Classroom-Friendly ABO Blood Types Kit: Blood Agglutination Simulation

    Arnold, Savittree Rochanasmita; Kruatong, Tussatrin; Dahsah, Chanyah; Suwanjinda, Duongdearn

    2012-01-01

    The classroom-friendly ABO blood type kit was developed by combining advantages of modelling and a simulation laboratory to teach the topics of ABO blood types and blood transfusion. Teachers can easily simulate the agglutination reaction on a blood type testing plate in the classroom, and show the students how this reaction occurs by using the…

  3. ABO blood group antigens in oral mucosa. What is new?

    Dabelsteen, Erik

    2002-01-01

    Histo-blood group ABH (O) antigens are major alloantigens in humans. These antigens are widely distributed in human tissues and undergo changes in expression during cellular differentiation and malignant development. The ABH antigens have been characterized as terminal disaccharide determinants...... healing show similarly decreased expression of A/B antigens on migrating epithelial cells. Some studies suggest that the relationship between expression of blood group antigens and cell motility can be explained by different degrees of glycosylation of integrins. Changes in ABO expression in tumours have...

  4. Arctic Boreal Vulnerability Experiment (ABoVE) Science Cloud

    Duffy, D.; Schnase, J. L.; McInerney, M.; Webster, W. P.; Sinno, S.; Thompson, J. H.; Griffith, P. C.; Hoy, E.; Carroll, M.

    2014-12-01

    The effects of climate change are being revealed at alarming rates in the Arctic and Boreal regions of the planet. NASA's Terrestrial Ecology Program has launched a major field campaign to study these effects over the next 5 to 8 years. The Arctic Boreal Vulnerability Experiment (ABoVE) will challenge scientists to take measurements in the field, study remote observations, and even run models to better understand the impacts of a rapidly changing climate for areas of Alaska and western Canada. The NASA Center for Climate Simulation (NCCS) at the Goddard Space Flight Center (GSFC) has partnered with the Terrestrial Ecology Program to create a science cloud designed for this field campaign - the ABoVE Science Cloud. The cloud combines traditional high performance computing with emerging technologies to create an environment specifically designed for large-scale climate analytics. The ABoVE Science Cloud utilizes (1) virtualized high-speed InfiniBand networks, (2) a combination of high-performance file systems and object storage, and (3) virtual system environments tailored for data intensive, science applications. At the center of the architecture is a large object storage environment, much like a traditional high-performance file system, that supports data proximal processing using technologies like MapReduce on a Hadoop Distributed File System (HDFS). Surrounding the storage is a cloud of high performance compute resources with many processing cores and large memory coupled to the storage through an InfiniBand network. Virtual systems can be tailored to a specific scientist and provisioned on the compute resources with extremely high-speed network connectivity to the storage and to other virtual systems. In this talk, we will present the architectural components of the science cloud and examples of how it is being used to meet the needs of the ABoVE campaign. In our experience, the science cloud approach significantly lowers the barriers and risks to organizations

  5. abo-cross: Hydrogen broadening cross-section calculator

    Barklem, P. S.; Anstee, S. D.; O'Mara, B. J.

    2015-07-01

    Line broadening cross sections for the broadening of spectral lines by collisions with neutral hydrogen atoms have been tabulated by Anstee & O'Mara (1995), Barklem & O'Mara (1997) and Barklem, O'Mara & Ross (1998) for s-p, p-s, p-d, d-p, d-f and f-d transitions. abo-cross, written in Fortran, interpolates in these tabulations to make these data more accessible to the end user. This code can be incorporated into existing spectrum synthesis programs or used it in a stand-alone mode to compute line broadening cross sections for specific transitions.

  6. Protoplasmic Incompatibility in PODOSPORA ANSERINA: a Possible Function for Incompatibility Genes

    Boucherie, Hélian; Bernet, Jean

    1980-01-01

    The suppression of protoplasmic incompatibility resulting from nonallelic gene interactions has been obtained by the coupled effect of mutations in the modA and modB genes (Bernet 1971). Due to their female sterility, modA modB strains provide an experimental tool to determine whether or not the mod and incompatibility loci are involved in a function other than protoplasmic incompatibility. Present results show that modA modB female sterility is a nonautonomous trait since heterokaryotic myce...

  7. Detection of incompatible properties without erasure

    Nisticó, Giuseppe

    2004-01-01

    In this work we show that in double-slit experiment properties incompatible with Which Slit property can be detected without erasing the knowledge of which slit each particle passes through and without affecting the point of impact on the final screen. A systematic procedure to find these particular properties is provided. A thought experiment which realizes this detection is proposed.

  8. Incompatibilities analysis in the accredited laboratory

    D. Szewieczek

    2008-06-01

    Full Text Available Purpose of the presented paper aimed at motivating the necessity of the accreditation of research and standardising laboratories as factors deciding about the competitive advantage of those organisations on the European Union market.Design/methodology/approach used for the research has covered the analyses of results of internal and external audits conducted in one of Polish accredited laboratories and estimation of the incompatibilities occurred.Findings of the carried out research are as follows: number and character of incompatibilities, which are exposed during internal and external audits, reflect size of organisation, where the management system is implemented, phase of implementation as well as the time of functioning.Practical implications refers to any organisation which has quality management system implemented as well as to any accredited laboratory using internal audits as an element of continuous improvement and treating incompatibilities not as something disqualifying the investigated area, but as an supporting element. Originality/value of the presented paper belongs to the methodology comprising the usage of internal audits’ results - proved incompatibilities - as a tool for obtaining and assuring the confidence in the management system.

  9. Prognostic Impact of ABO Blood Group on the Survival in Patients with Ovarian Cancer

    Zhou, Juan; Yang, Li-Chao; He, Zhen-Yu; Li, Fang-Yan; Wu, San-Gang; Sun, Jia-yuan

    2015-01-01

    Purpose: The impact of ABO blood group on the survival of patients with ovarian cancer remains uncertain. The aim of this study was to evaluate the prognostic value of the ABO blood group in ovarian cancer patients. Methods: 256 ovarian cancer patients who received a cytoreductive surgery were retrospectively reviewed. The prognostic impact of the ABO blood group with respect to overall survival (OS) was analyzed. Results: The median follow-up time was 57 months and the 5-year OS was 70.1%. T...

  10. Incompatibility boundaries for properties of community partitions

    Browet, Arnaud; Sarlette, Alain

    2016-01-01

    We prove the incompatibility of certain desirable properties of community partition quality functions. Our results generalize the impossibility result of [Kleinberg 2003] by considering sets of weaker properties. In particular, we use an alternative notion to solve the central issue of the consistency property. (The latter means that modifying the graph in a way consistent with a partition should not have counterintuitive effects). Our results clearly show that community partition methods should not be expected to perfectly satisfy all ideally desired properties. We then proceed to show that this incompatibility no longer holds when slightly relaxed versions of the properties are considered, and we provide in fact examples of simple quality functions satisfying these relaxed properties. An experimental study of these quality functions shows a behavior comparable to established methods in some situations, but more debatable results in others. This suggests that defining a notion of good partition in communitie...

  11. MOLECULAR ABO PHENOTYPING IN CYNOMOLGUS MACAQUES USING REAL TIME QUANTITATIVE PCR (QPCR)

    Premasuthan, Amritha; Ng, Jillian; Kanthaswamy, Sreetharan; Trask, Jessica Satkoski; Houghton, Paul; Farkas, Tibor; Sestak, Karol; Smith, David Glenn

    2012-01-01

    Macaques are commonly used in biomedical research as animal models of human disease. The ABO phenotype of donors and recipients plays an important role in the success of transplantation and stem cell research of both human and macaque tissue. Traditional serological methods for ABO phenotyping can be time consuming, provide ambiguous results and/or require tissue that is unavailable or unsuitable. We developed a novel method to detect the A, B, and AB phenotypes of macaques using real-time qu...

  12. ABO Genotype, ‘Blood-Type’ Diet and Cardiometabolic Risk Factors

    Jingzhou Wang; Bibiana García-Bailo; Nielsen, Daiva E.; Ahmed El-Sohemy

    2014-01-01

    BACKGROUND: The 'Blood-Type' diet advises individuals to eat according to their ABO blood group to improve their health and decrease risk of chronic diseases such as cardiovascular disease. However, the association between blood type-based dietary patterns and health outcomes has not been examined. The objective of this study was to determine the association between 'blood-type' diets and biomarkers of cardiometabolic health and whether an individual's ABO genotype modifies any associations. ...

  13. DISTRIBUTION OF ABO BLOOD GROUP AND RH(D) FACTOR IN WESTERN RAJASTHAN

    DR. RAJSHREE BEHRA; DR. YOGI RAJ JOSHI

    2013-01-01

    India is a vast country with lot of diversity in race, religion & creed. The same diversity has been observed in geographical distribution of blood groups in population within country. The ABO & Rhesus (Rh) blood group system are the most prevalent & important for transfusion of blood & its component, organ transplant, genetic studies & in legal medicine study. This study presents data about the prevalence of ABO & Rhesus (Rh) blood groups amongst blood donors during a period of ten & half ye...

  14. ABO and Rh-D blood group frequency and distribution: a tertiary care hospital experience

    Pandu Rangarao Sanagapati; Sudhir Kumar Vujhini

    2015-01-01

    Background: This study was carried out to determine the frequency and distribution of ABO and Rhesus (Rh) blood groups in blood donors of tertiary care hospital-attached blood bank. Methods: This study was conducted among the blood donors of Hyderabad Telangana State (India), who donated blood during the period from July 2013 to December 2014. ABO and Rh blood grouping was performed by forward blood grouping (cell grouping) using test tube agglutination method using antisera A, antisera B...

  15. An integrative evolution theory of histo-blood group ABO and related genes.

    Yamamoto, Fumiichiro; Cid, Emili; Yamamoto, Miyako; Saitou, Naruya; Bertranpetit, Jaume; Blancher, Antoine

    2014-01-01

    The ABO system is one of the most important blood group systems in transfusion/transplantation medicine. However, the evolutionary significance of the ABO gene and its polymorphism remained unknown. We took an integrative approach to gain insights into the significance of the evolutionary process of ABO genes, including those related not only phylogenetically but also functionally. We experimentally created a code table correlating amino acid sequence motifs of the ABO gene-encoded glycosyltransferases with GalNAc (A)/galactose (B) specificity, and assigned A/B specificity to individual ABO genes from various species thus going beyond the simple sequence comparison. Together with genome information and phylogenetic analyses, this assignment revealed early appearance of A and B gene sequences in evolution and potentially non-allelic presence of both gene sequences in some animal species. We argue: Evolution may have suppressed the establishment of two independent, functional A and B genes in most vertebrates and promoted A/B conversion through amino acid substitutions and/or recombination; A/B allelism should have existed in common ancestors of primates; and bacterial ABO genes evolved through horizontal and vertical gene transmission into 2 separate groups encoding glycosyltransferases with distinct sugar specificities. PMID:25307962

  16. The Construal (In)compatibility Effect

    Yang, Xiaojing; Ringberg, Torsten; Mao, Huifang;

    2011-01-01

    This research examines how consumers with a creative mind-set are persuaded by advertising claims construed at different levels (i.e., abstract vs. concrete ad claims). Across four experiments, we show that consumers with a creative mindset are more persuaded by ad claims construed at a level...... incompatible with their mental construal, while ad claims construed at a level compatible with consumers' mental construal are more effective for those who possess a less creative mindset. We document that such differences in persuasion are driven by the fact that consumers with a creative (less creative) mind...

  17. The Incompatibility of Living Systems: Characterizing Growth-Induced Incompatibilities in Expanded Skin.

    Buganza Tepole, Adrian; Gart, Michael; Purnell, Chad A; Gosain, Arun K; Kuhl, Ellen

    2016-05-01

    Skin expansion is a common surgical technique to correct large cutaneous defects. Selecting a successful expansion protocol is solely based on the experience and personal preference of the operating surgeon. Skin expansion could be improved by predictive computational simulations. Towards this goal, we model skin expansion using the continuum framework of finite growth. This approach crucially relies on the concept of incompatible configurations. However, aside from the classical opening angle experiment, our current understanding of growth-induced incompatibilities remains rather vague. Here we visualize and characterize incompatibilities in living systems using skin expansion in a porcine model: We implanted and inflated two expanders, crescent, and spherical, and filled them to 225 cc throughout a period of 21 days. To quantify the residual strains developed during this period, we excised the expanded skin patches and subdivided them into smaller pieces. Skin growth averaged 1.17 times the original area for the spherical and 1.10 for the crescent expander, and displayed significant regional variations. When subdivided into smaller pieces, the grown skin patches retracted heterogeneously and confirmed the existence of incompatibilities. Understanding skin growth through mechanical stretch will allow surgeons to improve-and ultimately personalize-preoperative treatment planning in plastic and reconstructive surgery. PMID:26416721

  18. Mechanisms of self-incompatibility and unilateral incompatibility in diploid potato (Solanum tuberosum L.).

    Eijlander, R.

    1998-01-01

    In chapter 1 an overview is given of the major mechanisms operating in Angiosperms that prevent or limit the degree of inbreeding. The two major systems that function on the basis of interaction between pollen and stigma/style, are the sporophytic and the gametophytic self-incompatibility systems (S

  19. Relationship between ABO blood groups and carcinoma of esophagus and cardia in Chaoshan inhabitants of China

    Min Su; Shan-Ming Lu; Dong-Ping Tian; Hu Zhao; Xiao-Yun Li; DeRui Li; Zhi-Chao Zheng

    2001-01-01

    AIM To study the relationship between ABO blood groups and carcinoma of esophagus and cardia in Chaoshan inhebitants of China, which is a unique Littoral high-risk area of esophageal carcinoma in China. The poor communication and transportation in the psst has made Cheoshan a relatively closed area and kept its culture and costure of old China thousend years ago.``METHODS Data on age, sex, ABO blood type and X-rayor psthological diagnose of the pstients with carcinoma of esophagus or cardia were collected from the Tumor Hospital. First Affiliated Hospital, Second Affiliated Hospital of Shantou University Medical College; and the Central Hospital of Shantou and the Central Hospital of Jieyang. A total of 6685 pstients with esophageal carcinoma (EC) and 2 955 patients with cardiac cancer (CC) in Chaoshen district were retrospectively assessed for their association with ABO blood groups.``RESULTS The distribution of ABO blood groups in patients with EC or CC was similar to the norrnal local population in Chaoshen. However, blood group B in male patients with CC and in the pstients with carcinoma in the upper third esophagus was 2.3% and 4.7% higher than the corresponding controls. The relative risk B: O was 1. 1415 (P<0.05)and 1 .2696 (P<0.05), respectively. No relationship was found between ABO blood groups and tumor differentiation.``CONCLTUSION ABO blood group B is associated with the incidence of CC in male individuals and carcinona in the upper third esophagus. The distribution of ABO blood groups varies in the different geographical and ethnic groups. As a result, proper controls are very important for such studies.``

  20. Non-association between anti-Toxoplasma gondii antibodies and ABO blood group system

    ACF Rodrigues

    2011-01-01

    Full Text Available Toxoplasma gondii infects humans through the gastrointestinal tract (GIT, which elicits humoral immune response with specific antibodies. The expression of the ABO blood group glycoconjugates also occurs in this same system and may influence the human susceptibility of infection by T. gondii. The aim of the present study was to investigate the association between ABO blood group phenotypes and the presence of anti-T. gondii antibodies. Data - including age, results of serology tests for T. gondii infection and ABO blood group phenotypes - were assembled from the medical records of 1,006 pregnant women attended in the Base Hospital of the Medical School of São José do Rio Preto, Brazil, between 2001 and 2004. The chi-square test was used to compare the results with the level of significance set at 5%. Of the studied cases, 64.1% (645/1006 and 35.9% (391/1006 presented respectively positive and negative serology tests for anti-T. gondii antibodies. The mean age of those who tested positive was higher than those with negative serology tests (p = 0.0004. The frequencies of ABO blood group phenotypes were similar in those with and without anti-T. gondii antibodies (p = 0.35. In conclusion, the ABO blood group system is not associated with the presence or absence of anti-T. gondii antibodies.

  1. Plasmodial incompatibility in the myxomycetes: a review

    Haskins EF

    2012-03-01

    Full Text Available Two myxomycete phaneroplasmodia of the same species undergo somatic fusion only if they are phenotypically identical for a complex genetic incompatibility system. This system consists of a three tiered polygenic complex with dominant and recessive alleles. Thus, plasmodia must be phenotypically identical for approximately 16 loci in order to fuse (CC and Cc are phenotypically identical, but different from cc. The first level of the system (having a minimum of seven Fus loci controls membrane fusion, and it apparently prevents fusion unless the two plasmodia have identical membrane or slime sheath components. The second level (having a minimum of six Cz loci produces a rapid lysis of a small mixed region, of the two plasmodia, if membrane fusion has occurred. This lysis is directional in that it targets the recessive phenotype, and it is apparently triggered by some pre-formed substances when they come into contact with a different plasmodium. The third level (having a minimum of three Let loci comes into play if membrane fusion occurs and there is no rapid lysis of the mixed plasmodium. It produces a slow lethal reaction, which targets and degrades the nuclei of the recessive phenotype. This reaction occurs over a period of five to twenty hours and requires the synthesis of new RNA and proteins. Since, this complex system produces a minimum of 65,536 different incompatibility phenotypes, it is highly unlikely that any two phaneroplasmodia will undergo a successful fusion unless they are very closely related. Species with aphaneroplasmoida apparently have a similar system, but species with small protoplasmodia do not appear to undergo any type of plasmodial fusion.

  2. A STUDY OF DISTRIBUTION OF ABO AND RH BLOOD GROUPS SYSTEM AMONG BLOOD DONORS AT A TERTIARY CARE HOSPITAL

    Rajesh Kumar; Puja; Vivek Vikram; Amit; Shweeta; Sawke; Shalaka; Nilima

    2015-01-01

    Up till now about 400red cells antigen have been identified. The majority are inherited by Mendelian fashion. The ABO and Rh blood group system was first to be identified and is most important for blood transfusion purposes. OBJECTIVE: This study was conducted to determine the frequency of ABO and Rhesus (Rh) blood groups in a ter...

  3. Incompatible insect technique: incompatible males from a Ceratitis capitata genetic sexing strain

    Wolbachia are obligatory intracellular and maternally inherited bacteria that infect and spread through natural arthropod populations by inducing male-killing, feminization, parthenogenesis and, most commonly, unidirectional and bidirectional cytoplasmic incompatibility (CI). Cytoplasmic incompatibility can be used to control natural populations of insect pests, in a way analogous to the sterile insect technique (SIT), namely through the incompatible insect technique (IIT). For the successful application of the ITT (based on a unidirectional CI approach) against a target species, it is essential that only males are released, as the release of females would lead to fertile matings between the released males and the released females and the establishment of a Wolbachia-carrying field population. In the present study, we describe a Wolbachia-infected line of the VIENNA 8 genetic sexing strain of the Mediterranean fruit fly, Ceratitis capitata (Wiedermann) (Diptera: Tephritidae), that carries the selectable marker temperature sensitive lethal (tsl). We show that (1) transferred Wolbachia induce high levels of CI even after the temperature treatment required for the male-only production, and (2) the Wolbachia-infected genetic sexing C. capitata line can be used in cage population suppression experiments analogous to the SIT. We also discuss our results in a comparison between IIT and SIT, investigating whether irradiation and cytoplasmic factors can be combined toward the development of novel strategies for insect pest control. (author)

  4. Frequency of ABO/Rhesus Blood Groups in Patients with Diabetes Mellitus.

    Oner, Can; Dogan, Burcu; Telatar, Berrin; Celik Yagan, Canan Fidan; Oguz, Aytekin

    2016-01-01

    The correlation between ABO/Rh blood groups and diabetes mellitus is still controversial. The aim of this study was to determine the relationship between ABO/Rhesus blood groups and diabetes in Turkish population. This cross-sectional study was conducted in Istanbul Medeniyet University Göztepe Education and Training Hospital's Diabetes Units. The study group was composed of 421 patients with type-1 diabetes, 484 patients with type-2 diabetes and 432 controls. Blood samples were collected and tested for ABO/Rhesus blood groups. Data was analyzed by SPSS version 17.0. Asignificant association was found between blood groups and diabetes mellitus. The frequency of AB blood group was significantly higher in type-1 diabetics; and Ablood group was significantly higher in type-2 diabetics. Furthermore, Rh negativity were significantly more frequent in type-2 diabetics. PMID:26787039

  5. Evolution and Molecular Control of Hybrid Incompatibility in Plants

    Chen, Chen; E, Zhiguo; Lin, Hong-Xuan

    2016-01-01

    Postzygotic reproductive isolation (RI) plays an important role in speciation. According to the stage at which it functions and the symptoms it displays, postzygotic RI can be called hybrid inviability, hybrid weakness or necrosis, hybrid sterility, or hybrid breakdown. In this review, we summarized new findings about hybrid incompatibilities in plants, most of which are from studies on Arabidopsis and rice. Recent progress suggests that hybrid incompatibility is a by-product of co-evolution either with “parasitic” selfish elements in the genome or with invasive microbes in the natural environment. We discuss the environmental influences on the expression of hybrid incompatibility and the possible effects of environment-dependent hybrid incompatibility on sympatric speciation. We also discuss the role of domestication on the evolution of hybrid incompatibilities. PMID:27563306

  6. Genetic Analysis of ABO and Rh Blood Groups in Backward Caste Population of Uttar Pradesh, India

    Rai, Vandana; Kumar, Pradeep

    2011-01-01

    A series of glycoproteins and glycolipids on red blood cell surface constitute blood group antigens. These are AB, A, B and O in ABO blood group system and Rh in rhesus blood group system. A total of 1065 unrelated Backward Caste (OBC) individuals from Uttar Pradesh were studied for the phenotype and allele frequency distribution of ABO and Rh (D) blood groups. Total 1065 samples analyzed, phenotype B blood type has the highest frequency 36.81% (n=392), followed by O (32.68%; n=348), A (23.66...

  7. Abo and Rh Blood Groups Distribution in Hemophilia and Anti Hiv Positive Individuals

    D.D. FARHUD

    1987-07-01

    Full Text Available A group of Iranian patients suffering from Factor VIII deficiency (Hemophilia A and treated with contaminated coagulation factor (imported, became seropositive as determined by ELISA method. Sixty of these individuals, which were available, were studied for ABO distribution. The B blood group in anti HIV pos. individuals (13.33% shows a significant decrease in comparison with the total (1504 of factor VIII hemophilia (21.87%. Statistical analysis of ABO distribution in anti HIV Pos. compared with hemophilia A and the control group showed x2 values of 6.86(0.10 > p>0.05 and 10.21(0.02> P >0.01 respectively.

  8. ABO Blood Group Genotyping by Real-time PCR in Kazakh Population

    Pavel Tarlykov

    2014-12-01

    Full Text Available Introduction. ABO blood group genotyping is a new technology in hematology that helps prevent adverse transfusion reactions in patients. Identification of antigens on the surface of red blood cells is based on serology; however, genotyping employs a different strategy and is aimed directly at genes that determine the surface proteins. ABO blood group genotyping by real-time PCR has several crucial advantages over other PCR-based techniques, such as high rapidity and reliability of analysis. The purpose of this study was to examine nucleotide substitutions differences by blood types using a PCR-based method on Kazakh blood donors.Methods. The study was approved by the Ethics Committee of the National Center for Biotechnology. Venous blood samples from 369 healthy Kazakh blood donors, whose blood types had been determined by serological methods, were collected after obtaining informed consent. The phenotypes of the samples included blood group A (n = 99, B (n = 93, O (n = 132, and AB (n = 45. Genomic DNA was extracted using a salting-out method. PCR products of ABO gene were sequenced on an ABI 3730xl DNA analyzer (Applied Biosystems. The resulting nucleotide sequences were compared and aligned against reference sequence NM_020469.2. Real-time PCR analysis was performed on CFX96 Touch™ Real-Time PCR Detection System (BioRad.Results. Direct sequencing of ABO gene in 369 samples revealed that the vast majority of nucleotide substitutions that change the ABO phenotype were limited to exons 6 and 7 of the ABO gene at positions 261, 467, 657, 796, 803, 930 and 1,060. However, genotyping of only three of them (261, 796 and 803 resulted in identification of major ABO genotypes in the Kazakh population. As a result, TaqMan probe based real-time PCR assay for the specific detection of genotypes 261, 796 and 803 was developed. The assay did not take into account several other mutations that may affect the determination of blood group, because they have a

  9. On microcontinuum field theories: the Eshelby stress tensor and incompatibility conditions

    Lazar, Markus; Maugin, Gerard A.

    2015-01-01

    We investigate linear theories of incompatible micromorphic elasticity, incompatible microstretch elasticity, incompatible micropolar elasticity and the incompatible dilatation theory of elasticity (elasticity with voids). The incompatibility conditions and Bianchi identities are derived and discussed. The Eshelby stress tensor (static energy momentum) is calculated for such inhomogeneous media with microstructure. Its divergence gives the driving forces for dislocations, disclinations, point...

  10. Evolution of Wolbachia cytoplasmic incompatibility types.

    Dobson, Stephen L

    2004-10-01

    The success of obligate endosymbiotic Wolbachia infections in insects is due in part to cytoplasmic incompatibility (CI), whereby Wolbachia bacteria manipulate host reproduction to promote their invasion and persistence within insect populations. The observed diversity of CI types raises the question of what the evolutionary pathways are by which a new CI type can evolve from an ancestral type. Prior evolutionary models assume that Wolbachia exists within a host individual as a clonal infection. While endosymbiotic theory predicts a general trend toward clonality, Wolbachia provides an exception in which there is selection to maintain diversity. Here, evolutionary trajectories are discussed that assume that a novel Wolbachia variant will co-exist with the original infection type within a host individual as a superinfection. Relative to prior models, this assumption relaxes requirements and allows additional pathways for the evolution of novel CI types. In addition to describing changes in the Wolbachia infection frequency associated with the hypothesized evolutionary events, the predicted impact of novel CI variants on the host population is also described. This impact, resulting from discordant evolutionary interests of symbiont and host, is discussed as a possible cause of Wolbachia loss from the host population or host population extinction. The latter is also discussed as the basis for an applied strategy for the suppression of insect pest populations. Model predictions are discussed relative to a recently published Wolbachia genome sequence and prior characterization of CI in naturally and artificially infected insects. PMID:15562682

  11. Des loyautés incompatibles

    Daniel Bizeul

    2007-06-01

    Full Text Available Faire l’expérience directe des phénomènes, en participant aux activités des milieux concernés, représente un des moyens les plus naturels et les plus sûrs pour atteindre la complexité du monde social. C’est aussi un des moyens les plus perturbants pour le chercheur, souvent confronté aux interdits de contact entre des groupes dont les intérêts ou les principes de vie sont antagonistes. Établir des relations étroites avec des militants du Front national, certains d’entre eux « néo-nazis », certains vivant à quelques rues de chez soi, tout en étant « homo », avec pour proches amis des hommes de couleur, de plus entouré de personnes hostiles à ce parti, oblige ainsi à gérer des liens inconciliables. Un risque possible pour le chercheur est alors d’être soumis à des sentiments contradictoires et de mettre du temps pour aboutir à une analyse exempte de toute influence indue.Incompatible loyalties. Moral aspects of an immersion into the French National Front Party.Experiencing directly phenomenon while participating to activities of the concerned circles, represents one of the most natural and safer way to reach the complexity of the society. It is also one of the most disturbing means for the researcher, often confronted to interdicts of contacts between groups which interests or principles of life are antagonists. Creating close relationships with French National Front party activists, some of them “neo-nazis”, some of them living a few streets from your house, while being gay, with colored men as closed friends, surrounded by persons hostile to this party, thus forces to make opposed connections. A possible risk for the researcher can be to be divided between contradictory feelings and spending time finishing an analysis free of unwarranted influence.Lealtades incompatibles. Problemas morales derivados de los contactos con el « Frente nacional »Establecer un contacto directo con fenómenos de diferente

  12. A Laboratory Exercise to Determine Human ABO Blood Type by Noninvasive Methods

    Martin, Michael P.; Detzel, Stephen M.

    2008-01-01

    Analysis of single-nucleotide polymorphisms and their association with diseases and nondisease phenotypes is of growing importance in human biology studies. In this laboratory exercise, students determine the genetic basis for their ABO blood type; however, no blood is drawn. Students isolate genomic DNA from buccal mucosa cells that are present…

  13. Association of ABO Blood Group Phenotype and Allele Frequency with Chikungunya Fever

    Rujirojindakul, Pairaya; Chongsuvivatwong, Virasakdi; Limprasert, Pornprot

    2015-01-01

    Background. The objective of this study was to investigate the association of the ABO blood group phenotype and allele frequency with CHIK fever. Methods. A rural community survey in Southern Thailand was conducted in August and September 2010. A total of 506 villagers were enrolled. Cases were defined as individuals having anti-CHIK IgG by hemagglutination ≥1 : 10. Results. There were 314 cases (62.1%) with CHIK seropositivity. Females were less likely to have positive anti-CHIK IgG with odds ratio (OR) (95% CI) of 0.63 (0.43, 0.93). All samples tested were Rh positive. Distribution of CHIK seropositivity versus seronegativity (P value) in A, B, AB, and O blood groups was 80 versus 46 (0.003), 80 versus 48 (0.005), 24 versus 20 (0.55), and 130 versus 78 (<0.001), respectively. However, chi-square test between ABO and CHIK infection showed no statistical significance (P = 0.76). Comparison of the ABO blood group allele frequency between CHIK seropositivity and seronegativity was not statistically significant. Conclusion. This finding demonstrated no association of the ABO blood group phenotypes and allele frequencies with CHIK infection. PMID:25977691

  14. Clinical use of the ABO-Scoring Index: reliability and subtraction frequency.

    Lieber, William S; Carlson, Sean K; Baumrind, Sheldon; Poulton, Donald R

    2003-10-01

    This study tested the reliability and subtraction frequency of the study model-scoring system of the American Board of Orthodontists (ABO). We used a sample of 36 posttreatment study models that were selected randomly from six different orthodontic offices. Intrajudge and interjudge reliability was calculated using nonparametric statistics (Spearman rank coefficient, Wilcoxon, Kruskal-Wallis, and Mann-Whitney tests). We found differences ranging from 3 to 6 subtraction points (total score) for intrajudge scoring between two sessions. For overall total ABO score, the average correlation was .77. Intrajudge correlation was greatest for occlusal relationships and least for interproximal contacts. Interjudge correlation for ABO score averaged r = .85. Correlation was greatest for buccolingual inclination and least for overjet. The data show that some judges, on average, were much more lenient than others and that this resulted in a range of total scores between 19.7 and 27.5. Most of the deductions were found in the buccal segments and most were related to the second molars. We present these findings in the context of clinicians preparing for the ABO phase III examination and for orthodontists in their ongoing evaluation of clinical results. PMID:14580024

  15. Genetic and epigenetic alterations of the blood group ABO gene in oral squamous cell carcinoma

    Gao, Shan; Worm, Jesper; Guldberg, Per;

    2004-01-01

    Loss of histo-blood group A and B antigen expression is a frequent event in oral carcinomas and is associated with decreased activity of glycosyltransferases encoded by the ABO gene. We examined 30 oral squamous cell carcinomas for expression of A and B antigens and glycosyltransferases. We also...

  16. Thermodynamic incompatibility and complex formation in pectin/caseinate mixtures

    Rediguieri, Camila F; de Freitas, Osvaldo; Lettinga, Pavlik; Tuinier, Remco

    2007-01-01

    The instability of polysaccharide/protein mixtures occurs because of either thermodynamic incompatibility or complexation. We studied which instability mechanism dominated given the external conditions. Therefore the effect of temperature, pH, and biopolymer concentration on the phase separation of pectin/caseinate mixtures was investigated. At pH > 6, thermodynamic incompatibility with spinodal decomposition was observed in pectin/caseinate mixtures resulting in the formation of water-in-wat...

  17. Breaking Gaussian incompatibility on continuous variable quantum systems

    Heinosaari, Teiko, E-mail: teiko.heinosaari@utu.fi [Turku Centre for Quantum Physics, Department of Physics and Astronomy, University of Turku, FI-20014 Turku (Finland); Kiukas, Jukka, E-mail: jukka.kiukas@aber.ac.uk [Department of Mathematics, Aberystwyth University, Penglais, Aberystwyth, SY23 3BZ (United Kingdom); Schultz, Jussi, E-mail: jussi.schultz@gmail.com [Turku Centre for Quantum Physics, Department of Physics and Astronomy, University of Turku, FI-20014 Turku (Finland); Dipartimento di Matematica, Politecnico di Milano, Piazza Leonardo da Vinci 32, I-20133 Milano (Italy)

    2015-08-15

    We characterise Gaussian quantum channels that are Gaussian incompatibility breaking, that is, transform every set of Gaussian measurements into a set obtainable from a joint Gaussian observable via Gaussian postprocessing. Such channels represent local noise which renders measurements useless for Gaussian EPR-steering, providing the appropriate generalisation of entanglement breaking channels for this scenario. Understanding the structure of Gaussian incompatibility breaking channels contributes to the resource theory of noisy continuous variable quantum information protocols.

  18. Breaking Gaussian incompatibility on continuous variable quantum systems

    We characterise Gaussian quantum channels that are Gaussian incompatibility breaking, that is, transform every set of Gaussian measurements into a set obtainable from a joint Gaussian observable via Gaussian postprocessing. Such channels represent local noise which renders measurements useless for Gaussian EPR-steering, providing the appropriate generalisation of entanglement breaking channels for this scenario. Understanding the structure of Gaussian incompatibility breaking channels contributes to the resource theory of noisy continuous variable quantum information protocols

  19. Noise robustness of the incompatibility of quantum measurements

    Heinosaari, Teiko; Kiukas, Jukka; Reitzner, Daniel

    2015-08-01

    The existence of incompatible measurements is a fundamental phenomenon having no explanation in classical physics. Intuitively, one considers given measurements to be incompatible within a framework of a physical theory, if their simultaneous implementation on a single physical device is prohibited by the theory itself. In the mathematical language of quantum theory, measurements are described by POVMs (positive operator valued measures), and given POVMs are by definition incompatible if they cannot be obtained via coarse-graining from a single common POVM; this notion generalizes noncommutativity of projective measurements. In quantum theory, incompatibility can be regarded as a resource necessary for manifesting phenomena such as Clauser-Horne-Shimony-Holt (CHSH) Bell inequality violations or Einstein-Podolsky-Rosen (EPR) steering which do not have classical explanation. We define operational ways of quantifying this resource via the amount of added classical noise needed to render the measurements compatible, i.e., useless as a resource. In analogy to entanglement measures, we generalize this idea by introducing the concept of incompatibility measure, which is monotone in local operations. In this paper, we restrict our consideration to binary measurements, which are already sufficient to explicitly demonstrate nontrivial features of the theory. In particular, we construct a family of incompatibility monotones operationally quantifying violations of certain scaled versions of the CHSH Bell inequality, prove that they can be computed via a semidefinite program, and show how the noise-based quantities arise as special cases. We also determine maximal violations of the new inequalities, demonstrating how Tsirelson's bound appears as a special case. The resource aspect is further motivated by simple quantum protocols where our incompatibility monotones appear as relevant figures of merit.

  20. ABO and Rh Blood Type Relationship in Parents with more than One Disabled Child

    Mehrmohammadi, M

    2015-01-01

    Background Parental blood variables are one of the most important medical-biological causes of intellectual and physical-movement disabilities. The purpose of this study was to identify the relationship between parents’ blood variables (ABO and Rh blood type) and their relationship with frequency of intellectual and physical-movement disabilities in Isfahan province. Materials and Methods This was a descriptive-analytical study and 494 samples were selected from mothers with more than one dis...

  1. ABO blood type is associated with endometrial cancer risk in Chinese women

    Xu, Wang-hong; Zheng, Wei; Xiang, Yong-Bing; Shu, Xiao-Ou

    2011-01-01

    ABO blood type has been associated with risk of several malignancies. However, results are not consistent. In this population-based case-control study including 1204 incident endometrial cancer cases and 1212 population controls, we examined the association of self-reported Serologic blood type with endometrial cancer risk using a logistic regression model. Women with endometrial cancer were more likely to have blood type A. Compared to women with blood type O, the adjusted odds ratios for en...

  2. Research study of diversity of Rh and ABO blood groups in 110 psoriatic patients

    Valieghanie M

    1996-01-01

    Psoriasis is an common, chronic, recurrent, inflammatory disease of the skin, characterized by red scaling plaques on the skin surface. The morphology of psoriatic lesions allows classification of the different types of psoriasis that included plaque type, pustular type and Erythrodermic type. I have studied the relationship between distribution of RH and ABO blood groups in 110 psoriatic patients and compared with control normal blood groups. The result of this study was as follow: The rate ...

  3. Breast cancer incidence in Greek women in relation to ABO blood groups and Rh factor

    Stamatakos, Michael; Kontzoglou, Konstantinos; Safioleas, Panagiotis; Safioleas, Constnatinos; Manti, Christina; Safioleas, Michael

    2009-01-01

    Aim To investigate the correlation between breast cancer in Greek women and ABO blood groups. Material-methods In 166 female patients with breast cancer factors such as blood group, histological type, family history, presence or absence of nodal and/or distant metastases were examined. These patients had similar demographic, clinical, surgical, immunohistochemical, laboratory, and follow-up data and this group is representative of general population of women in Greece. Results The ductal type...

  4. Mortality and cancer in relation to ABO blood group phenotypes in the Golestan Cohort Study

    Etemadi, Arash; Kamangar, Farin; Islami, Farhad; Poustchi, Hossein; Pourshams, Akram; Brennan, Paul; Boffetta, Paolo; Malekzadeh, Reza; Dawsey, Sanford M.; Abnet, Christian C.; Emadi, Ashkan

    2015-01-01

    Background A few studies have shown an association between blood group alleles and vascular disease, including atherosclerosis, which is thought to be due to the higher level of von Willebrand factor in these individuals and the association of blood group locus variants with plasma lipid levels. No large population-based study has explored this association with overall and cause-specific mortality. Methods We aimed to study the association between ABO blood groups and overall and cause-specif...

  5. No Association of Phenotypic ABO Blood Group and Malaria during Pregnancy

    Boel, Machteld E.; Marcus J Rijken; Pimanpanarak, Mupawjay; Keereecharoen, Naw Lily; Proux, Stephane; Nosten, François; McGready, Rose

    2012-01-01

    In a few small studies an association between blood group O and placental malaria has been described. The relationship between blood group and malaria in pregnancy (Plasmodium vivax and Plasmodium falciparum) was analyzed in 1,468 women from three longitudinal cohort studies in which weekly malaria screening was done systematically during pregnancy. One-third of women (447 of 1,468) had at least one malaria infection in pregnancy. The ABO blood group phenotype was not associated with the spec...

  6. Land Use Effects on Soil Quality Indicators: A Case Study of Abo-Wonsho Southern Ethiopia

    Fantaw Yimer; Melku Dagnachew; Awdenegest Moges

    2013-01-01

    Soil quality assessment is valuable for evaluating agroecosystem sustainability, soil degradation, and identifying sustainable land management practices. This study compared soil quality within culturally protected forest areas and adjacent grassland, grazing land, and farmland in Abo-Wonsho, Southern Ethiopia. A total of 40 soil samples (4 land uses × 5 replications × 2 soil depth layers: 0 to 10 cm and 10 to 20 cm) were collected for analysis. Soil textural fractions (i.e., sand, silt, and ...

  7. Heterogeneity and diversity of ABO and Rh blood group genes in select Saudi Arabian populations.

    AlSuhaibani, E S; Kizilbash, N A; Malik, S

    2015-01-01

    In order to investigate the diversity of ABO and Rh blood group genes in the Saudi Arabian population, we assembled the phenotypic data of approximately 66,000 subjects from ten representative Saudi populations: Al-Khobar, Riyadh, Tabuk/Madina Al-Munawaara, Jeddah, Abha, South region, Sakaka, Domah, Al-Qurayat, and Sweer. The frequencies of p[A], q[B], and r[O] alleles at the ABO locus were observed to be 0.1688, 0.1242, and 0.7070, respectively, and the frequency of the D allele at the Rh locus was 0.7138. The heterozygosities at the ABO and Rh loci were 0.4563 and 0.4086, respectively, while the combined heterozygosity was 0.4324. Homogeneity tests revealed the population of Abha to be the most heterogeneous while that of Tabuk/Madina was found to be the least heterogeneous. Homogeneity was higher among the Northern populations while Southern populations demonstrated subdivisions and stratification. Gene diversity analyses yielded a total heterozygosity value of 0.4449. The coefficient of gene differentiation was 0.0090. Nei's genetic distance analyses showed that there was close affinity between the populations of Al-Khobar and Riyadh. The largest differences were observed between the populations of Sakaka and Domah. Furthermore, negative correlations were found between p[A] and r[O] alleles, and between q[B] and r[O] alleles at the ABO locus. Clinal analyses revealed that the r[O] allele showed an increasing trend from North-East to South-West, and conversely the q[B] allele exhibited a decreasing trend at these coordinates. These analyses present interesting aspects of the blood group allele distribution across the geography of Saudi Arabia. PMID:26214466

  8. Helicobacter pylori infection according to ABO blood group among blood donors in Kosovo

    Bukurije Zhubi; Zana Baruti-Gafurri; Ymer Mekaj; Mimoza Zhubi; Idriz Merovci; Iliriane Bunjaku; Valdete Topciu; Emine Devoli-Disha

    2011-01-01

    Introduction: Numerous studies have reported a high prevalence of Helicobacter pylori infection among healthy and non-healthy persons in different places. The Aim of the study is to investigate the seroprevalence of H. pylori infection among Kosovo’s Blood donor associated with ABO/Rhesus blood group.Methods: 671 blood donors are tested for H. pylori antibodies and results are classifi ed by way of donation, age, gender, blood groups and education level. Serum antibodies are analyzed by Enzym...

  9. Les mélanges de polymères incompatibles Incompatible Polymer Mixtures

    Serpe G.

    2006-11-01

    Full Text Available Les mélanges de polymères thermoplastiques font actuellement l'objet d'un effort considérable de recherche et de développement. Cette synthèse bibliographique concerne principalement les mélanges mécaniques de polymères incompatibles à l'état fondu. Ceux-ci ont une morphologie hétérogène qui dépend des conditions de mise en oeuvre autant que de la nature des constituants. De plus, pour ce type de matériaux, la nature de l'interface joue un rôle déterminant sur la formation de la morphologie hétérogène et donc sur les propriétés du produit final. D'autre part un problème important réside dans la stabilité des mélanges réalisés. L'ajout d'une faible quantité d'agent tensioactif de type copolymère séquencé, ou greffé, ou la modification chimique des constituants (greffage ou réticulation par exemple permet, dans certains cas d'améliorer très sensiblement les propriétés des mélanges en augmentant l'adhérence entre les phases immiscibles. L'ensemble de ces considérations permettent la réalisation industrielle de nouveaux matériaux polymères aux caractéristiques spécifiques. Thermoplastic polymer mixtures are currently the subject of considerable research and development efforts. This bibliographic synthesis deals mainly with melt blending process of incompatible polymers. These blends have a heterogeneous morphology that depends on the processing conditions as much as on the chemical structure of the componants. Likewise, for these materials the nature of the interface plays a decisive role on the formation of the heterogeneous morphology and hence on the properties on the end product. Furthermore, an important problem is to stabilize the morphology of the blends. Adding a small amount of surfactant of the sequenced of grafted copolymer type or, else, the chemical reaction of the constituents (e. g. grafting or branching during processing are ways in some cases of considerably improving the properties of

  10. DISTRIBUTION OF ABO BLOOD GROUP AND RH(D FACTOR IN WESTERN RAJASTHAN

    DR. RAJSHREE BEHRA

    2013-02-01

    Full Text Available India is a vast country with lot of diversity in race, religion & creed. The same diversity has been observed in geographical distribution of blood groups in population within country. The ABO & Rhesus (Rh blood group system are the most prevalent & important for transfusion of blood & its component, organ transplant, genetic studies & in legal medicine study. This study presents data about the prevalence of ABO & Rhesus (Rh blood groups amongst blood donors during a period of ten & half year (retrospective study from January 2002 to July 2012 conducted at Blood Bank of Mahatma Gandhi Hospital, Jodhpur associated with Dr. S. N. Medical College, Jodhpur (India. During the study period blood groups of 83631 donors were screened by antigen antibody agglutination method using commercially available antiseras which were validated at our Blood Bank. The study revealed that the commonest ABO blood group was B, followed by O, A and AB respectively. Rh negative were 8.25%. [Natl J of Med Res 2013; 3(1.000: 73-75

  11. Genetic Analysis of ABO and Rh Blood Groups in Backward Caste Population of Uttar Pradesh, India

    Vandana RAI

    2011-08-01

    Full Text Available A series of glycoproteins and glycolipids on red blood cell surface constitute blood group antigens. These are AB, A, B and O in ABO blood group system and Rh in rhesus blood group system. A total of 1065 unrelated Backward Caste (OBC individuals from Uttar Pradesh were studied for the phenotype and allele frequency distribution of ABO and Rh (D blood groups. Total 1065 samples analyzed, phenotype B blood type has the highest frequency 36.81% (n=392, followed by O (32.68%; n=348, A (23.66%; n=252 and AB (6.85%; n=73. The overall phenotypic frequencies of ABO blood groups were B>O>A>AB. The allelic frequencies of O, A, and B alleles were 0.5819, 0.1674 and 0.2506 respectively. Out of total 1065 samples, 1018 (95.59% samples were Rh-positive and 47 (4.41% were Rh-negative. Phenotypic frequency of Rh-negative in Koari, Yadav, Kurmi and Maurya samples were 0.99%, 4%, 1.4% and 7.6% respectively.

  12. Pancreatic cancer risk variant ABO rs505922 in patients with cholangiocarcinoma

    Marcin Krawczyk; Florentina Mihalache; Aksana H(o)blinger; Monica Acalovschi; Frank Lammert; Vincent Zimmer

    2011-01-01

    The aim of this study was to investigate an association between the development of cholangiocarcinoma (CCA) and the ABO variant rs505922 (known to increase pancreatic cancer risk) in a large cohort of European individuals with CCA. In total, 180 individuals with CCA and 350 CCA-free controls were included. The ABO variant rs505922 was genotyped using a polymerase chain reaction-based assay. Association between this single nucleotide polymorphism (SNP) and CCA was tested in contingency tables. Neither allele distributions nor association tests and regression analysis provided evidence for an increased risk of CCA among carriers of the ABO variant (all P > 0.05). Nevertheless, we documented a deviation from Hardy-Weinberg equilibrium in the entire CCA cohort (P = 0.028) and for patients with intrahepatic (P = 0.037) but not extrahepatic tumor localization (P > 0.05). The association tests did not provide evidence for a prominent role of the investigated SNP in the genetic risk of CCA. However, Hardy-Weinberg disequilibrium in the entire cohort and the intrahepatic CCA subgroup warrants future studies investigating a potential CCA risk modulation by individual blood groups.

  13. ABO blood groups and oral premalignancies: A clinical study in selected Indian population

    S Bhateja

    2014-01-01

    Full Text Available Background: The ABO blood group antigens are present on the surface of red blood cells and various epithelial cells. As the majority of human cancers are derived from epithelial cells, changes in blood group antigens constitute an important aspect of human cancers. The aim of the study was to establish clinical usefulness of ABO blood group as a predisposing factor in early diagnosis and management of patients with oral precancerous lesions/conditions. Materials and Methods: The study sample consisted of 50 control and 50 oral precancer (25 leukoplakia and 25 Oral Submucous Fibrosis confirmed by histopathologic examination. All samples were subjected to blood group testing and their prevalence was compared by Z-test using STATA version 8. Results: The "A" blood group was prevalent among the precancerous group. Significant differences on prevalences of blood groups were found (P < 0.05 between control versus leukoplakia and OSMF. Interestingly, 24% gutka chewers who had higher number of grades of dysplasia were falling in "A" blood group. Conclusion: Blood group type should be considered along with other risk factors to understand the individual patient′s risk and further studies in larger samples with inclusion of Rh factor is needed to elucidate the relationship with ABO blood group types.

  14. Distribution of ABO, Rh, MNSs, Kell and Duffy blood-group antigens in population of Vojvodina

    Vojvodić Svetlana

    2003-01-01

    Full Text Available Introduction Analysis of erythrocyte blood group antigen polymorphisms and genetic variability in population of Vojvodina was performed by investigating gene and genotype frequencies which determine antigens of ABO, Rh, MNSs, Kell and Duffy blood-group systems. Material and methods We investigated 350 unrelated persons from Vojvodina in regard to appurtenance of ABO, Rh, MNSs, Kell and Duffy blood-group systems. We calculated gene, genotype, phenotype frequencies and proportion significance test. Results and discussion Results of investigation revealed that gene and genotype frequencies of investigated blood-group systems are similar to corresponding data for majority of European populations, while statistically significant differences were established in inhabitants of geographically distant regions. Values of proportion significance test revealed statistically significant differences of genotype frequencies for ABO and MNSs blood-group antigens in populations of: Australian Aborigines, Chinese population, Arabians, Blacks, Eskimos, American Indians (Navaho and Pueblo and population of Papua New Guinea. Statistically significant differences of genotype frequencies were established in inhabitants of narrow geographical areas of Europe such as: Finland, Germany, Sweden, Albania, England and Netherlands. Conclusion Our results point to the fact that erythrocyte blood-groups have different frequencies in some parts of the world, and that there are great differences in frequencies of some blood-groups among inhabitants of various continents and races. Genetical peculiarity of the population of Vojvodina points to the fact that differences in blood-group frequencies are also present among inhabitants of narrow geographical areas.

  15. ABO blood groups in relation to breast carcinoma incidence and associated prognostic factors in Moroccan women.

    Zouine, S; Marnissi, F; Otmani, N; Bennani Othmani, M; El Wafi, M; Kojok, K; Zaid, Y; Tahiri Jouti, N; Habti, N

    2016-07-01

    The association between blood groups ABO and different types of diseases was established in several previous studies. Our aim was to seek the possible association between the ABO blood group and breast cancer-associated prognostic factors. The Chi-squared analytic test was used to compare phenotypic ABO distribution among Moroccan blood donors and 442 cases of women suffering from breast carcinoma with archived files in Maternity Ward of University Hospital C.H.U Ibn Rochd between 2008 and 2011. High incidence of breast carcinoma was observed in blood type B patients (p type B was associated with breast carcinomas overexpressing human epidermal growth factor receptor HER2 (p risk of cancer at age over 70 years (p type A was associated with high risk of cancer among women younger than 35 years old. Blood type A and AB were associated with high incidence of lymph node metastasis (p type and estrogen receptor-positive tumor. Patients with blood group A, B, and AB were more likely to develop aggressive breast carcinoma. Further follow-up studies are necessary to clarify the role of ABH antigens in the progression of breast carcinoma. PMID:27241035

  16. Association of ABO and Colton Blood Group Gene Polymorphisms With Hematological Traits Variation.

    Shahbazi, Shirin; Mashayekhi, Amir; Fatahi, Neda; Mahdavi, Mohammad-Reza

    2015-12-01

    Hematological parameters are appraised routinely to determine overall human health and to diagnose and monitor certain diseases. In GWASs, more than 30 loci carrying common deoxyribonucleic acid (DNA) polymorphisms have been identified related to hematological traits. In this study, we investigated the contribution of ABO rs2073823 along with AQP1 rs1049305 and rs10244884 polymorphisms in hematological traits variation in a cohort of Iranian healthy individuals.Genomic DNA was extracted from peripheral blood of 168 healthy volunteer. Genotyping was performed by ARMS-PCR or PCR-RFLP and confirmed by DNA sequencing. Complete blood analyses were conducted for the participants. Significant association was observed between AQP1 rs1049305 and the hematological traits including hemoglobin, hematocrit, and platelet count (P = 0.012, 0.008, and 0.011, respectively). The AQP1 rs10244884 status was also significantly linked to hemoglobin and hematocrit levels in the study cohort (P = 0.015 and 0.041, respectively). Furthermore, ABO rs2073823 polymorphism was identified as a hemoglobin and hematocrit levels modifier (both with P = 0.004).AQP1 and ABO variants appear to predict hemoglobin and hematocrit levels but not other erythrocyte phenotype parameters including red blood cell counts and red blood cell indices. PMID:26632894

  17. Detention and incompatibility of HIV patients in Italy

    Serena Dell'Isola

    2013-12-01

    Full Text Available In Italy, Law 231/99 and subsequent amendments standardize the conditions allowing or not a HIV positive inmate to remain in jail. Currently such clinical conditions are not automatically associated with the decline of preventive detention and the Court evaluates the incompatibility with detention on the basis of two additional and independent criteria. We have been observing the tendency by jailed HIV-positive patients to manipulate the disease state believing that the rules of incompatibility with the prison system are always applied. The management of HIV positive patients in jail involves significant sanitary and relational efforts, particularly for those suffering AIDS and/or with severe immunodeficiency.

  18. Relation between ABO blood groups and Helicobacter pylori infection in symptomatic patients

    Jaff MS

    2011-09-01

    Full Text Available Mohamad Salih Jaff Pathology Department, College of Medicine, Hawler Medical University (formerly Salahuddin University, Erbil, Kurdistan Region, Iraq Abstract: Epidemiological studies have demonstrated higher frequencies of the O blood group and the nonsecretor phenotype of ABH antigens among patients suffering from peptic ulcers. Since Helicobacter pylori has been established as the main etiological factor in this disease, controversies about the associations of the ABO and Lewis blood group phenotypes and secretor and nonsecretor phenotypes in relation to susceptibility towards infection by this bacillus have been presented. The aim of this study was to verify the frequencies of ABO and Rhesus (Rh blood groups in H. pylori seropositive symptomatic patients. The study included (n = 1108 patients with dyspepsia symptoms referred from an outpatient clinic in Erbil city for investigation. Age, sex, and residency were recorded as a routine laboratory framework. Patients underwent SD Bioline (Standard Diagnostics Inc, Kyonggi-do, South Korea and enzyme-linked immunosorbent assay serologic tests for H. pylori. ABO blood group phenotypes were determined by a standard hemagglutination test. Results showed that 64.8% of patients (n = 718/1108 were seropositive for H. pylori infection, and (35.2% (n = 390/1108 were seronegative. Of the seropositive patients, 40.8% (n = 293/718 were male and 59.2% (n = 425/718 were female; while of the seronegative patients, 46.7% (n = 182/390 were male and 53.3% (n = 208/390 were female. The mean age for seropositives and seronegatives was (38.0 ± 14.6 years and (37.6 ± 15.7 years respectively. The frequency of the ABO and Rh-positive (Rh+ blood groups among seropositive patients was (A = 32.0%, B = 19.5%, AB = 6.7%, O = 41.8%, and Rh+ = 92.5% and was (A = 32.3%, B = 28.2%, AB = 8.0%, O = 31.5%, and Rh+ = 92.5% in seronegatives. The results of this study suggest that ABO blood groups, age, and gender influence

  19. 26. Relationship between ABO Blood Groups and Carcinoma of Esophagus and Cardia in Chaoshan Littoral of China

    2001-01-01

    Abstract: Background. Chaoshan is the only littoral among the six high-risk areas of esophageal carcinoma (EC) in China and the relatively isolatal society for the inconvenient transport gives an opportunity to study the genetics of carcinoma of esophagus and cardia. Some reports had suggested that ABO blood groups were associated with tumors, but their relation remained controversial. Methods: The data of age, sex, ABO blood type and X-ray or pathological diagnosis of the patients with carcinoma of esophagus or cardia were collected from The tumor hospital, First affiliated hospital, Second affiliated hospital of Shantou University Medical College; the Center hospital of Shantou and the Center hospital of Jieyang. In this study, 6 685 patients with EC and 2 955 patients with cardiac cancer(CC) in Chaoshan district were retrospectively assessed the association with ABO blood groups. Results: The distribution of ABO blood groups in patients with EC or CC was similar to the normal local population in Chaoshan. However, there was 2.3% excess of blood group B in male patients with CC and 4.7% excess in the patients with carcinoma in the upper third esophagus, compared with the corresponding controls. The relative risk B:O was 1.1415 (P<0.05) and 1.2696 (P<0.05), respectively No relationship between ABO blood groups and tumor differentiation was found. Conclusion: ABO blood group B was associated with the incidence of CC in male individuals and carcinoma in the upper third esophagus. The distribution of ABO blood groups varied in the different geographical and ethnic groups, as a result, proper controls were very important for such study.

  20. MIXING OF INCOMPATIBLE MATERIALS IN WASTE TANKS TECHNICAL BASIS DOCUMENT

    This document presents onsite radiological, onsite toxicological, and offsite toxicological consequences, risk binning, and control decision results for the mixing of incompatible materials in waste tanks representative accident. Revision 4 updates the analysis to consider bulk chemical additions to single shell tanks (SSTs)

  1. Variance-based uncertainty relations for incompatible observables

    Chen, Bin; Cao, Ning-Ping; Fei, Shao-Ming; Long, Gui-Lu

    2016-06-01

    We formulate uncertainty relations for arbitrary finite number of incompatible observables. Based on the sum of variances of the observables, both Heisenberg-type and Schrödinger-type uncertainty relations are provided. These new lower bounds are stronger in most of the cases than the ones derived from some existing inequalities. Detailed examples are presented.

  2. Information complementarity: A new paradigm for decoding quantum incompatibility

    Zhu, Huangjun

    2015-09-01

    The existence of observables that are incompatible or not jointly measurable is a characteristic feature of quantum mechanics, which lies at the root of a number of nonclassical phenomena, such as uncertainty relations, wave—particle dual behavior, Bell-inequality violation, and contextuality. However, no intuitive criterion is available for determining the compatibility of even two (generalized) observables, despite the overarching importance of this problem and intensive efforts of many researchers. Here we introduce an information theoretic paradigm together with an intuitive geometric picture for decoding incompatible observables, starting from two simple ideas: Every observable can only provide limited information and information is monotonic under data processing. By virtue of quantum estimation theory, we introduce a family of universal criteria for detecting incompatible observables and a natural measure of incompatibility, which are applicable to arbitrary number of arbitrary observables. Based on this framework, we derive a family of universal measurement uncertainty relations, provide a simple information theoretic explanation of quantitative wave—particle duality, and offer new perspectives for understanding Bell nonlocality, contextuality, and quantum precision limit.

  3. ABO and Rh (D group distribution and gene frequency; the first multicentric study in India

    Amit Agrawal

    2014-01-01

    Full Text Available Background and Objectives: The study was undertaken with the objective to provide data on the ABO and Rh(D blood group distribution and gene frequency across India. Materials and Methods: A total of 10,000 healthy blood donors donating in blood banks situated in five different geographical regions of the country (North, South, East and Center were included in the study. ABO and Rh (D grouping was performed on all these samples. Data on the frequency of ABO and Rh(D blood groups was reported in simple numbers and percentages. Results: The study showed that O was the most common blood group (37.12% in the country closely followed by B at 32.26%, followed by A at 22.88% while AB was the least prevalent group at 7.74%. 94.61% of the donor population was Rh positive and the rest were Rh negative. Regional variations were observed in the distribution. Using the maximum likelihood method, the frequencies of the I A , I B and I O alleles were calculated and tested according to the Hardy Weinberg law of Equilibrium. The calculated gene frequencies are 0.1653 for I A (p, 0.2254 for I B (q and 0.6093 for I O (r. In Indian Population, O (r records the highest value followed by B (q and A (p; O > B > A. Conclusion: The study provides information about the relative distribution of various alleles in the Indian population both on a pan-India basis as well as region-wise. This vital information may be helpful in planning for future health challenges, particularly planning with regards to blood transfusion services.

  4. ABO Blood Group and Dementia Risk--A Scandinavian Record-Linkage Study.

    Senthil K Vasan

    Full Text Available Dementia includes a group of neuro-degenerative disorders characterized by varying degrees of cognitive impairment. Recent data indicates that blood group AB is associated with impaired cognition in elderly patients. To date there are no large-scale studies that have examined the relationship between ABO blood group and dementia-related disorders in detail.We used data from the SCANDAT2 database that contains information on over 1.6 million blood donors from 1968 in Sweden and 1981 from Denmark. The database was linked with health outcomes data from nationwide patient and cause of death registers to investigate the relationship between blood groups and risk of different types of dementia. The incident rate ratios were estimated using log-linear Poisson regression models.Among 1,598,294 donors followed over 24 million person-years of observation we ascertained 3,615 cases of Alzheimer's disease, 1,842 cases of vascular dementia, and 9,091 cases of unspecified dementia. Overall, our study showed no association between ABO blood group and risk of Alzheimer's disease, vascular dementia or unspecified dementia. This was also true when analyses were restricted to donors aged 70 years or older except for a slight, but significantly decreased risk of all dementia combined in subjects with blood group A (IRR, 0.93; 95% confidence interval [CI], 0.88-0.98, compared to those with blood group O.Our results provide no evidence that ABO blood group influences the risk of dementia.

  5. Distribution and clinal trends of the ABO and Rh genes in select Middle Eastern countries.

    AlSuhaibani, E S; Kizilbash, N A; Afshan, K; Malik, S

    2015-01-01

    An understanding of the ABO and Rh blood group systems is important for blood transfusions and is also pertinent due to their potential association with certain morbidities and susceptibilities to infections. To investigate the diversity and differentiation of the ABO and Rh loci in Middle Eastern populations, data from twelve representative Middle Eastern populations were analyzed. Six populations were in conformity with Hardy-Weinberg equilibrium at the ABO locus. The pooled heterozygosity at both loci was calculated to be highest in the sample from Jordan and lowest in Bahrain. Heterogeneity was pronounced in the Northern compared to the Southern Middle Eastern populations. Overall, the absolute gene diversity was 0.0046 and gene differentiation was calculated to be 0.0100. Genetic diversity of the studied loci across all populations (HT) was estimated to be 0.4594, while the diversity within the populations (HS) was 0.4548. Nei's genetic distance analyses revealed highest affinities between the populations of Kuwait and Qatar, Oman and Yemen, and between Qatar and the United Arab Emirates. These results were displayed through a UGPMA dendrogram and principal component analyses, which established clustering of certain populations. Clinal trends of the allelic systems were observed by generating contour maps that allow a detailed appreciation of the distributions of alleles across the geography of the Arabian Peninsula and the Middle East. Taken together, these analyses are helpful in understanding the differentiation of blood group loci and for designing prospective studies for establishing the associations of these loci with health variables in the populations studied. PMID:26400302

  6. Prevalence of hemoglobinopathy, ABO and rhesus blood groups in rural areas of West Bengal, India

    Bikash Mondal

    2012-01-01

    Full Text Available Background: Hemoglobinopathies are a group of inherited disorders of hemoglobin synthesis. It could be formed a fatal scenario in concern of lacking of actual information. Beside this, ABO and Rh blood grouping are also important matter in transfusion and forensic medicine and to reduce new born hemolytic disease (NHD. Materials and Methods: The spectrum and prevalence of various hemoglobinopathies, ABO and rhesus (Rh blood groups was screened among patients who visited B.S. Medical College and Hospital, Bankura, West Bengal, India. This study was carried out on 958 patients of different ages ranging from child to adults from January to June 2011. High-performance liquid chromatography (HPLC, complete blood count (CBC and hemagglutination technique were performed for the assessment of abnormal hemoglobin variants, ABO and Rh blood groups, respectively. Results: Results from this study had been shown that there was high prevalence of hemoglobinpathies (27.35% where β-thalassemia in heterozygous state occurred more frequent than other hemoglobinopathies. Out of 958 patients, 72.65% were HbAA and 27.35% were hemoglobinopathies individuals where 17.64% β-thalassemia heterozygous, 2.92% β-thalassemia homozygous, 3.86% HbAE, 1.15% HbAS trait, 1.25% HbE-β thalassemia trait and 0.52% HbS-β thalassemia trait were found. No incidence of HbSS, HbSC, HbCC, HbD and other variants of hemoglobinpathies were observed. The gene frequencies with respect to ABO systems had been shown as O > B > A > AB. Blood group O was the highest (35.8% and the least percentage distribution was blood group AB (6.68%. Rhesus positive (Rh+ were 97.7%, while the remaining was 2.3% Rhesus negative (Rh-. The frequencies of A + , B + , AB +, and O + blood groups were 22.44%, 33.61%, 6.58%, and 35.07%, respectively. Conclusions: Remarkable percentages of hemoglobinopathies were prevalent from the present study. An extensive screening of the population is needed to assess the

  7. XPS study of surface absorbed oxygen of ABO3 mixed oxides

    ZHAO Xin; YANG Qiuhua; CUI Jinjin

    2008-01-01

    Perovskite-type complex oxides ABO3 (A=Sr, La;B=Mn, Fe, Co) were prepared by citric acid method. The degradation of water-solubilized dyes was carried out using the mixed oxides as photocatalyst. The surface absorbed oxygen was analyzed using X-ray photoelectron spectroscopy (XPS). The results indicated that there was a relationship between the photocatalytic activity and the content of the surface absorbed oxygen. The higher the content of the surface absorbed oxygen was, the better the performance of the photocatalyst.

  8. ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging

    Toemsak Srikhirin; Armote Somboonkaew; Ratthasart Amarit; Boonsong Sutapun; Mongkol Kunakorn; Pimpun Kitpoka; Krisda Sudprasert; Patjaree Peungthum; Nongluck Houngkamhang; Apirom Vongsakulyanon

    2013-01-01

    In this study, readily available antibodies that are used in standard agglutination tests were evaluated for their use in ABO blood typing by a surface plasmon resonance imaging (SPR imaging) technique. Five groups of antibodies, including mixed clones of anti-A, anti-B, and anti-AB, and single clones of anti-A and anti-B, were used to construct the five-line detection arrays using a multichannel flow cell in the SPR imager. The red blood cell (RBC) samples were applied to a multichannel flow...

  9. The prevalence of transfusion transmitted infections in ABO blood groups and Rh type system

    Jitendra Singh Nigam; Savitri Singh; Viplesh Kaur; Sumit Giri; Ravi Prakash Kaushal

    2014-01-01

    Screening of blood and blood products is important to reduce the risk of transfusion transmitted infections (TTIs). The transfusion of unscreened or inadequately screened blood and blood products are the major source of TTIs. The aim of this paper is to find out the prevalence of TTIs in ABO blood groups and Rh type system. A total of 4128 blood donors were screened from January 2010 to April 2014. Serological tests were performed for hepatitis B surface antigen (HBsAg), anti hepatitis C viru...

  10. Frequencies of ABO Gene and Rh Blood Groups in Hamadan, Iran

    Hamid Pour-Jafari; Morteza Hashemzadeh Chaleshtari; Mohammad Reza Imani

    2011-01-01

    Background: Determining of gene frequencies of A, Band 0 genes from ABO blood group system and D and d genes from Rhesus system are objectives of the present study.

    <...

  11. Electron paramagnetic resonance of Cr3+ ions in ABO3 (A = Sc, Lu, In) diamagnetic crystals

    Vorotynov, A. M.; Ovchinnikov, S. G.; Rudenko, V. V.; Vorotynova, O. V.

    2016-04-01

    A magnetic resonance method is applied to the investigation of a number of isostructural diamagnetic compounds ABO3 (A = Sc, Lu, In) with small additions of Cr3+ ions ( S = 3/2) sufficient to observe single-ion spectra. It is shown that the resonance spectra for isolated Cr3+ ions can be described to a good accuracy by the ordinary axial spin Hamiltonian for 3 d ions in octahedral oxygen environment. The parameters of the spin Hamiltonian are determined. It is established that Cr3+ ions in these crystals are characterized by easy-axis-type anisotropy.

  12. Understanding the mechanisms of chemical incompatibility ammonium nitrate by molecular modeling

    Cagnina, Stefania

    2014-01-01

    Numerous chemical reactants tend to lead to undesired phenomena of incompatibility. In order to prevent the happening of those phenomena, a rapid and accurate identification of the incompatibilities is needed. Until now, experimental studies, which provide insightful, but limited information, were the only possible approach used for the study of incompatibilities. In this work a molecular modeling approach was used in order to complete and improve experimental results of incompatibility proce...

  13. Incompatible strategies in international mergers: the failed merger between Telia and Telenor

    Christine Benedichte Meyer; Ellen Altenborg

    2008-01-01

    The aim of this paper is to explore the problems of incompatible strategies in international mergers. Studying a failed merger between the two state-owned Scandinavian telecom corporations, we examine how the parties' strategies were incompatible. We find that the parties' strategies were incompatible in three distinctive areas, and study how the companies tried to resolve these incompatibilities. Owing to national governance structures established to protect national interests, the parties w...

  14. Covalent Fusion of layered Incompatible Gels in Immiscible Solvents

    Biswas, Santidan; Singh, Awaneesh; Matyjaszewski, Krzysztof; Balazs, Anna C.

    We carry out dissipative particle dynamics (DPD) simulations to model a two layered stackable gel where the gels are incompatible and are present in immiscible solvent. The bottom layer of the gel is created first and then a solution of new initiators, monomers and cross-linkers is introduced on top of it. These components then undergo polymerization and form the second gel layer. We study all possible combinations of free radical polymerization (FRP) and atom transfer radical polymerization (ATRP) mechanisms with the two layers of the gel. For example, the bottom layer gel is created via ATRP, whereas the top layer gel follows FRP. Our focus is to do a systematic study of all these combinations and find out the factors responsible for combining two incompatible gels in immiscible solvents.

  15. Cytomechanical properties of papaver pollen tubes are altered after self-incompatibility challenge

    Geitmann, A.; McConnaughey, W.; Lang-Pauluzzi, I.; Franklin-Tong, V.E.; Emons, A.M.C.

    2004-01-01

    Self-incompatibility (SI) in Papaver rhoeas triggers a ligand-mediated signal transduction cascade, resulting in the inhibition of incompatible pollen tube growth. Using a cytomechanical approach we have demonstrated that dramatic changes to the mechanical properties of incompatible pollen tubes are

  16. Distyly and heteromorphic self-incompatibility of Hedyotis pulcherrima (Rubiaceae)

    Xinxin Liu; Xiaoqin Wu; Dianxiang Zhang

    2012-01-01

    Heterostyly is a genetically controlled floral polymorphism, which includes both distyly and tristyly. We investigated morph ratio, floral and pollen morphology, and self-incompatibility of Hedyotispulcherrima. Overall, the natural population of H. pulcherrima was isoplethic, containing long-styled and short-styled morphs with an equilibrium 1:1 ratio. Long-styled and short-styled morph exhibited a precise reciprocal herkogamy, which was significantly correlated with corolla length. Stigma-lo...

  17. Incidence of intravenous drug incompatibilities in intensive care units

    Machotka, O.; Maňák, J.; Kuběna, Aleš Antonín; Vlček, J.

    2015-01-01

    Roč. 159, č. 4 (2015), s. 652-656. ISSN 1213-8118 Institutional support: RVO:67985556 Keywords : medical error * graph theory * graph coloring * drug administration * drug incompatibilities * applied combinatorics * decision theory * medical * medication safety * intensive care units Subject RIV: FN - Epidemiology, Contagious Diseases ; Clinical Immunology Impact factor: 1.200, year: 2014 http://library.utia.cas.cz/separaty/2014/E/kubena-0437509.pdf

  18. The different mechanisms of sporophytic self-incompatibility.

    Hiscock, Simon J; Tabah, David A

    2003-01-01

    Flowering plants have evolved a multitude of mechanisms to avoid self-fertilization and promote outbreeding. Self-incompatibility (SI) is by far the most common of these, and is found in ca. 60% of flowering plants. SI is a genetically controlled pollen-pistil recognition system that provides a barrier to fertilization by self and self-related pollen in hermaphrodite (usually co-sexual) flowering plants. Two genetically distinct forms of SI can be recognized: gametophytic SI (GSI) and sporoph...

  19. Hydroxypropyl Methylcellulose Acetate Succinate: Potential Drug–Excipient Incompatibility

    Dong, Zedong; Choi, Duk Soon

    2008-01-01

    The stability of hydroxypropyl methylcellulose acetate succinate (HPMC-AS) and its potential incompatibility with active pharmaceutical ingredients (API) carrying hydroxyl group(s) were investigated in this research. HPMC-AS may undergo hydrolysis under harsh processing conditions with the generation of succinic acid and acetic acid, which can form ester bond(s) with the hydroxyl group(s) in API. In this case, the hot-melt extrusion (HME) product prepared from HPMC-AS and our model compound (...

  20. How Incentive-Incompatible Deposit-Insurance Funds Fail

    Kane, Edward J.

    1989-01-01

    An incentive-incompatible deposit-insurance fund (IIDIF) is a scheme. Lot guaranteeing deposits at client institutions that deploys defective systems of information collection, client monitoring, and risk management. These defective systems encourage voluntary risk- taking by clients and by managers and politicians responsible for administering the fund. The paper focuses on how principal-agent conflicts and asymmetries in the distribution of information lead to myopic behavior by IIDIF manag...

  1. Comparative genetic organization of incompatibility group P degradative plasmids.

    Burlage, R S; Bemis, L A; Layton, A C; Sayler, G. S.; Larimer, F

    1990-01-01

    Plasmids that encode genes for the degradation of recalcitrant compounds are often examined only for characteristics of the degradative pathways and ignore regions that are necessary for plasmid replication, incompatibility, and conjugation. If these characteristics were known, then the mobility of the catabolic genes between species could be predicted and different catabolic pathways might be combined to alter substrate range. Two catabolic plasmids, pSS50 and pSS60, isolated from chlorobiph...

  2. On the Evolution of Genetic Incompatibility Systems. VI. a Three-Locus Modifier Model for the Origin of Gametophytic Self-Incompatibility

    Uyenoyama, M.K.

    1991-01-01

    Recent genetic analyses have demonstrated that self-incompatibility in flowering plants derives from the coordinated expression of a system of loci. To address the selective mechanisms through which a genetic system of this kind evolves, I present a three-locus model for the origin of gametophytic self-incompatibility. Conventional models assume that a single locus encodes all physiological effects associated with self-incompatibility and that the viability of offspring depends only on whethe...

  3. ABO blood type is associated with endometrial cancer risk in Chinese women

    Wang-Hong Xu; Wei Zheng; Yong-Bing Xiang; Xiao-Ou Shu

    2011-01-01

    ABO blood type has been associated with risk of several malignancies.However,results are not consistent.In this population-based case-control study including 1204 incident endometrial cancer cases and 1212 population controls,we examined the association of self-reported serologic blood type with endometrial cancer risk using a logistic regression model.Women with endometrial cancer were more likely to have blood type A.Compared to women with blood type O,the adjusted odds ratios for endometrial cancer were 1.00 [95% confidence interval (CI),0.79-1.28] for type B,1.24 (95% CI,0.90-1.69) for type AB,and 1.50 (95% CI,1.19-1.90) for type A.A significant dose-response relationship was observed for cancer risk and level of antigen A (P for trend =0.0003).The positive association of blood type A with cancer risk was observed regardless of menopausal status,body mass index,oral contraceptive use,or family cancer history.Our results suggest that ABO blood type may be involved in the development of endometrial cancer.

  4. ABO blood groups and Helicobacter pylori cagA infection: evidence of an association

    DE Mattos

    2010-01-01

    Full Text Available Diseases resulting from Helicobacter pylori infection appear to be dependent on a host of genetic traits and virulence factors possessed by this microorganism. This paper aimed to investigate the association between the ABO histo-blood groups and H. pylori cagA infections. Genomic DNA samples (n = 110 of gastric biopsies obtained from patients with endoscopic diagnosis of peptic ulcers (n = 25 and chronic active gastritis (n = 85 were analyzed by PCR using specific primers for the cagA gene. Of the samples, 66.4% (n = 73 tested positive and 33.6% (n = 37 negative for the gene. The cagA strain was predominant in peptic ulcers (n = 21; 84.0% compared with chronic active gastritis (n = 52; 61.2% (p = 0.05; OR 3.332; 95% CI: 1.050-10.576. Additionally, the cagA strain was prevalent in the type O blood (48/63; 76.2% compared with other ABO phenotypes (25/47; 53.2% (p = 0.01; OR 2.816; 95% CI: 1.246-6.364. These results suggest that H. pylori cagA infection is associated with the O blood group in Brazilian patients suffering from chronic active gastritis and peptic ulcers.

  5. Genetic of the ABO blood system and its link with the immune system A genética do sistema ABO e sua relação com o sistema imune

    Luiz C. de Mattos

    2004-03-01

    Full Text Available In the book "Eat Right For Your Type" the author Peter J. D'Adamo writes that the O blood type was the first blood type to appear in humans and affirms that the blood groups are the key to the immune system. Some recent phylogenetic network studies in humans and non-human primates implies that the A gene represents an ancient form of the ABO genes. Relationships between blood groups and infectious and noninfectious diseases and immunodeficiency abnormalities have also been reported in the literature. As D'Adamo's propositions seem to be in opposition with the current knowledge, we present in this paper some comments about the genetics and the evolution of the ABO blood group genes and some links between this blood system and the functioning of the immune system.Peter J. D'Adamo, autor do livro "Eat Right For Your Type", escreve que o grupo O representa o primeiro tipo sangüíneo que surgiu nos humanos e também afirma que os grupos sangüíneos constituem as bases do sistema imune. Recentes estudos filogenéticos realizados em primatas humanos e não humanos estabeleceram que o gene A representa a forma ancestral dos genes que ocupam o locus ABO. Associações entre os grupos sangüíneos ABO, doenças infecciosas, não infecciosas e imunodeficiências também foram relatadas. Diante das proposições do autor, as quais se opõem às informações resultantes de recentes estudos moleculares e filogenéticos, nossa intenção é apresentar algumas reflexões sobre a genética e a evolução dos genes do sistema ABO e as conexões deste sistema com o sistema imune.

  6. Assessing ABO/Rh Blood Group Frequency and Association with Asymptomatic Malaria among Blood Donors Attending Arba Minch Blood Bank, South Ethiopia.

    Alemu, Getaneh; Mama, Mohammedaman

    2016-01-01

    Background. Determination of the various ABO/Rh blood group distributions and their association with malaria infection has paramount importance in the context of transfusion medicine and malaria control. Methods. Facility based cross-sectional study was conducted from February to June, 2015, to assess ABO/Rh blood groups distribution and their association with asymptomatic malaria. A structured questionnaire was used to collect data. Blood grouping was done using monoclonal antibodies. Thin and thick blood films were examined for Plasmodium parasites. Data were analyzed using SPSS version 20.0. Results. A total of 416 blood donors participated with median age of 22 ± 0.29 (median ± standard error of the mean). Distribution of ABO phenotypes, in decreasing order, was O (175, 42.1%), A (136, 32.7%), B (87, 20.9%), and AB (18, 4.3%). Most of them were Rh+ (386, 92.8%). The overall malaria prevalence was 4.1% (17/416). ABO blood group is significantly associated with malaria infection (P = 0.022). High rate of parasitemia was seen in blood group O donors (6.899, P = 0.003) compared to those with other ABO blood groups. Conclusion. Blood groups O and AB phenotypes are the most and the least ABO blood groups, respectively. There is significant association between ABO blood group and asymptomatic malaria parasitemia. PMID:26925291

  7. Correlation between "ABO" blood group phenotypes and periodontal disease: Prevalence in south Kanara district, Karnataka state, India

    Gurpur Prakash Pai

    2012-01-01

    Full Text Available Background: The correlation between certain systemic diseases and ABO blood group is a well-documented fact. The association between periodontal disease and ABO blood group is not studied in relation to a specific geographic location. Here is a study conducted on a group of patients belonging to South Kanara district of Karnataka state. Materials and Methods: A total of 750 subjects aged between 30and 38 years belonging to South Kanara district were selected on random basis. The study subjects were segregated into healthy/mild gingivitis, moderate/severe gingivitis, and periodontitis group, based on Loe and Silness index and clinical attachment loss as criteria. The study group was further categorized and graded using Ramfjord′s periodontal disease index. Blood samples were collected to identify ABO blood group. Results: Prevalence of blood group O was more in South Kanara district, followed by blood groups B and A, and the least prevalent was AB. The percentage distribution of subjects with blood groups O and AB was more in healthy/mild gingivitis group (group I and moderate/severe gingivitis group (group II, while subjects with blood groups B and A were more in periodontitis group III. There was increased prevalence of subjects with blood groups O and AB with healthy periodontium, while subjects with blood groups B and A showed inclination toward diseased periodontium. Conclusion: There is a correlation existing between periodontal disease and ABO blood group in this geographic location. This association can be due to various blood group antigens acting as receptors for infectious agents associated with periodontal disease. This broad correlation between periodontal disease and ABO blood group also points toward susceptibility ofthe subjects with certain blood groups to periodontal disease.

  8. Is Quantum Mechanics Incompatible with Newton's First Law

    Rabinowitz, Mario

    2007-01-01

    Quantum mechanics (QM) clearly violates Newton's First Law of Motion (NFLM) in the quantum domain for one of the simplest problems, yielding an effect in a force-free region much like the Aharonov-Bohm effect. In addition, there is an incompatibility between the predictions of QM in the classical limit, and that of classical mechanics (CM) with respect to NFLM. A general argument is made that such a disparity may be found commonly for a wide variety of quantum predictions in the classical lim...

  9. Frequency of abo blood groups among the diabetes mellitus type 2 patients

    Objective: To study the frequency of ABO blood groups among diabetes mellitus type 2. Results: Comparison of blood groups frequency between the general population and diabetes type 2 patients was carried out in term of percentage. It was noticed that the values were 4.36, 17.15 and 7.34% higher for A, B and AB blood groups respectively in the diabetic patients. On the contrary, the value was 28.94% lower for the blood group O. Conclusion: Present study has supported the hypothesis that diabetes mellitus type 2 and blood groups are interrelated because of the broad genetic immunologic basis in both. It is concluded that the frequency of blood groups B and O is significantly higher and lower respectively in the diabetes mellitus type 2 patients as compared to the general population. (author)

  10. Blood groups polymorphism (ABO, Ss, Rhesus and Duffy) in the Arabic population of Beni Mellal

    The present study deals with anthropogenetic profile of the Arab speaking population of the Beni Mellal region which separates areas inhabited by Mid-Atlas Berbers from those inhabited by Soth-Morroccan Arabs. The study of blood groups ABO, Rhesus, Ss,and Duffy was conducted on 131 individuals. The result shows that this population has the highest frequencies of the FyO allele (0.860) and s allele (0.524) in comparison to all Arab and Berber populations of North Africa and the Middle East. However genetic distances estimated on the basis of these four markers reveal that the population of Beni Mellal and another in the Beni Hlal region are in the same sub-cluster with populations from the Middle East. This may be attributed to the Oriental Arab (Machrek) origin of these two Moroccan Arab populations. (author)

  11. Genetic Characterization of Human Populations: From ABO to a Genetic Map of the British People

    Bodmer, Walter

    2015-01-01

    From 1900, when Landsteiner first described the ABO blood groups, to the present, the methods used to characterize the genetics of human populations have undergone a remarkable development. Concomitantly, our understanding of the history and spread of human populations across the earth has become much more detailed. As has often been said, a better understanding of the genetic relationships among the peoples of the world is one of the best antidotes to racial prejudices. Such an understanding provides us with a fascinating, improved insight into our origins as well as with valuable information about population differences that are of medical relevance. The study of genetic polymorphisms has been essential to the analysis of the relationships between human populations. The evolution of methods used to study human polymorphisms and the resulting contributions to our understanding of human health and history is the subject of this Perspectives. PMID:25657345

  12. ABO Blood Group and Dementia Risk--A Scandinavian Record-Linkage Study

    Vasan, Senthil K; Rostgaard, Klaus; Ullum, Henrik;

    2015-01-01

    BACKGROUND: Dementia includes a group of neuro-degenerative disorders characterized by varying degrees of cognitive impairment. Recent data indicates that blood group AB is associated with impaired cognition in elderly patients. To date there are no large-scale studies that have examined...... the relationship between ABO blood group and dementia-related disorders in detail. METHODS: We used data from the SCANDAT2 database that contains information on over 1.6 million blood donors from 1968 in Sweden and 1981 from Denmark. The database was linked with health outcomes data from nationwide patient...... and cause of death registers to investigate the relationship between blood groups and risk of different types of dementia. The incident rate ratios were estimated using log-linear Poisson regression models. RESULTS: Among 1,598,294 donors followed over 24 million person-years of observation we ascertained 3...

  13. ABO and Rh-D blood group frequency and distribution: a tertiary care hospital experience

    Pandu Rangarao Sanagapati

    2015-08-01

    Results: Out of 1740 blood donors, 1702 (55.6% were male and 38 (44.4% were female. Majority of blood donors were in 21-40 years of age group. The most frequent blood group positions in the descending order are and lsquo;O', and lsquo;A', and lsquo;B' and and lsquo;AB' respectively. One group was and lsquo;Oh' (Bombay Phenotype. Conclusions: and lsquo;O' group is the most frequent position of ABO blood group system followed by and lsquo;A' group. Rh+ is the most frequent group than Rh- in the Rh system. Blood donations by females are very low. [Int J Res Med Sci 2015; 3(8.000: 2058-2061

  14. Darwin and the origin of interspecific genetic incompatibilities.

    Presgraves, Daven C

    2010-12-01

    Darwin's Origin of Species is often criticized for having little to say about speciation. The complaint focuses in particular on Darwin's supposed failure to explain the evolution of the sterility and inviability of interspecific hybrids. But in his chapter on hybridism, Darwin, working without genetics, got as close to the modern understanding of the evolution of hybrid sterility and inviability as might reasonably be expected. In particular, after surveying what was then known about interspecific crosses and the resulting hybrids, he established two facts that, while now taken for granted, were at the time radical. First, the sterility barriers between species are neither specially endowed by a creator nor directly favored by natural selection but rather evolve as incidental by-products of interspecific divergence. Second, the sterility of species hybrids results when their development is "disturbed by two organizations having been compounded into one." Bateson, Dobzhansky, and Muller later put Mendelian detail to Darwin's inference that the species-specific factors controlling development (i.e., genes) are sometimes incompatible. In this article, I highlight the major developments in our understanding of these interspecific genetic incompatibilities--from Darwin to Muller to modern theory--and review comparative, genetic, and molecular rules that characterize the evolution of hybrid sterility and inviability. PMID:21043780

  15. Proteomics analysis of compatibility and incompatibility in grafted cucumber seedlings.

    Xu, Qing; Guo, Shi-Rong; Li, Lin; An, Ya-Hong; Shu, Sheng; Sun, Jin

    2016-08-01

    Graft compatibility between rootstock and scion is the most important factor influencing the survival of grafted plants. In this study, we used two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS) to investigate differences in leaf proteomes of graft-compatible and graft-incompatible cucumber (Cucumis sativus L.)/pumpkin (Cucurbita L.) combinations. Cucumber seedlings were used as the scions and two pumpkin cultivars with strongly contrasting grafting compatibilities were used as the rootstocks. Non-grafted and self-grafted cucumber seedlings served as control groups. An average of approximately 500 detectable spots were observed on each 2-DE gel. A total of 50 proteins were differentially expressed in response to self-grafting, compatible-rootstock grafting, and incompatible-rootstock grafting and were all successfully identified by MALDI-TOF/TOF MS. The regulation of Calvin cycle, photosynthetic apparatus, glycolytic pathway, energy metabolism, protein biosynthesis and degradation, and reactive oxygen metabolism will probably contribute to intensify the biomass and photosynthetic capacity in graft-compatible combinations. The improved physiological and growth characteristics of compatible-rootstock grafting plants are the result of the higher expressions of proteins involved in photosynthesis, carbohydrate and energy metabolism, and protein metabolism. At the same time, the compatible-rootstock grafting regulation of stress defense, amino acid metabolism, and other metabolic functions also plays important roles in improvement of plant growth. PMID:27070289

  16. Determination of natural radioactive elements in Abo Zaabal, Egypt by means of gamma spectroscopy

    Highlights: ► We examined the radioactivity of different type samples from Abo Zaabaal Lake. ► We evaluated the natural nuclide gamma-ray activities and their annual dose rates. ► We evaluated the concentrations of 226Ra and its hazard indices. ► We assessed the absorbed dose in human. ► All results are within normal ranges. - Abstract: The natural nuclide gamma-ray activities and their respective annual effective dose rates, produced by 238U, 232Th, 40K and 226Ra, are determined for 10 different natural samples (soil–plant–water) from Abo Zaabaal Lake. This lake is located very close to the Egyptian reactors. The gamma spectra analysis indicates that the photo-gamma lines represent ten radioactive nuclides 234Th, 239Pu, 228Ac, 226Ra, 212Pb, 214Pb, 208Tl, 212Bi, 214Bi and 40K. These nuclides represent the daughters of the natural radioactive series 238U and 232Th with 40K. The mean activity concentration of 238U was found to be 6.57, 10.16 and 5.44 Bq kg−1 for (soil–plant–water); 8.46, 8.33 and 6.04 Bq kg−1 of 232Th, and 136.3, 216.8 and 119.2 Bq kg−1 of 40K respectively. The mean activity concentrations of 226Ra were obtained which help to evaluate the radiation hazard indices as radium equivalent, internal and external hazard indices. In addition, to assess the radiation risk to a biosystem, the annual effective dose rate, the absorbed dose in human and the absorbed dose outdoor are also evaluated.

  17. Paternity investigation among known false trios: ABO, Rh, MNSs, Kell, Duffy, Kidd, and HLA systems.

    Salaru, N N

    1993-11-01

    This paternity study was performed with trios in which the putative father was not the biological father (NBF), in order to evaluate adjustment of genetic markers employed to disclose non biological fathers for the population, and the biological meaning of likelihood of paternity in casework. All 923 generated trios had ABO, Rh, MNS, Kell and HLA systems tested; 372 of them also had Duffy and Kidd systems tested. The most powerful exclusion system was HLA, followed, in this order, by ABO, Rh, Duffy, MNSs, Kidd, and Kell. Taking into account the Indian/black/white historical miscegenation background in the population, an improvement in the performance of red blood cells as disclosers of non biological fathers could be achieved, if particular additional sera were used. In the group tested with seven different systems, direct exclusions were observed in 90.31%, and they were single system exclusions in 26.61%. In order to avoid the remote possibility of mutation, it is suggested that the number of used systems be increased. Indirect exclusions were verified in 8.87% and only 0.81% of NBF were not excluded at all. In this last group, probabilities of paternity were calculated and two values greater than 95% were obtained. To be able to accomplish the "visum et repertum" duty and to assist the court, the expert should equally emphasize: a) the probability of paternity of the alleged father and the possibility of finding an unexcluded NBF; b) the actual performance of systems used to uncover NBF, together with the probabilities of paternity of those who were not discovered; c) the previous referenced trend of probabilities of paternity of true and of non-biological fathers to cluster in distinct class intervals of likelihood of paternity. PMID:8263491

  18. Preliminary joint neutron time-of-flight and X-ray crystallographic study of human ABO(H) blood group A glycosyltransferase

    B. Schuman; Fisher, S. Z.; Kovalevsky, A.; Borisova, S. N.; Palcic, M. M.; Coates, L.; Langan, P.; Evans, S V

    2011-01-01

    Collection to 2.5 Å resolution of neutron diffraction data from crystals of the human ABO(H) blood group glycosyltransferase GTA, is presented with the preliminary joint refinement with the corresponding X-ray diffraction data.

  19. Graft Incompatibility Influence on Assimilating Pigments and Soluble Sugars Amount of some Pear (Pyrus sativa) Cultivars

    Gheorghii CIOBOTARI; Maria BRINZA; Aliona MORARIU; Gica GRADINARIU

    2010-01-01

    Graft incompatibility in fruit trees is one of the greatest obstacles in rootstocks and cultivars breeding. The mechanism in which incompatibility is expressed is not yet fully understood and several hypotheses have been advanced in an attempt to explain it. In many cases (pear on quince grafts, apricot on Prunus grafts), incompatibility is manifested by the breaking of the trees at the point of the union particularly when they have been growing for some years. Many reports focus on this prob...

  20. Drug incompatibilities in the adult intensive care unit of a university hospital

    Marsilio, Naiane Roveda; da Silva, Daiandy; Bueno, Denise

    2016-01-01

    Objectives This study sought to identify the physical and chemical incompatibilities among the drugs administered intravenously to patients admitted to an adult intensive care unit. We also aimed to establish pharmaceutical guidelines for administering incompatible drugs. Methods This cross-sectional, prospective, and quantitative study was conducted from July to September 2015. Drug incompatibilities were identified based on an analysis of the patient prescriptions available in the hospital ...

  1. Frequency of ABO and Rh (D) Blood Groups and Hemoglobin Threshold among Pregnant Women in Family Guidance Association, Mekelle Model Clinic, North Ethiopia.

    Megbaru Alemu; Guesh Abrha; Gessessew Bugssa; Kiros Tedla

    2014-01-01

    This study was aimed to determine the frequency of ABO and Rh blood group patterns and hemoglobin threshold among pregnant women screened at Family Guidance Association of Ethiopia, North Area Mekelle model clinic. Checklists were prepared to collect data from laboratory registration books of five years to undergo this retrospective study. Those registration books were reviewed for ABO blood group, Rh profiles and hemoglobin threshold of the pregnant women screened during the specified per...

  2. FREQUENCY OF ABO AND RHESUS (D) BLOOD GROUPS IN TRIBAL P OPULATION OF MADHYA PRADESH: A STUDY FROM TERTIARY CARE TEACHING HOSPITAL IN MADHYA PRADESH

    Ajit; Dolly

    2015-01-01

    BACKGROUND: ABO and Rh blood groups are most important blood groups in human beings. The frequency of four main blood group systems varies in population throughout the world and even in different parts of country. Objective if this study was to identify distribution o f ABO and Rh blood group system. MATERIALS AND METHODS: Blood samples from 10680 tribals were collected in Jhabua district of Madhya Pradesh during the month of June 2012. Among 10680 tribals, 5670 wer...

  3. Biochemical Models for S-Rnase-Based Self-Incompatibility

    Zhi-Hua Hua; Allison Fields; Teh-hui Kao

    2008-01-01

    S-RNase-based self-incompatibility (SI) is a genetically determined self/non-self-recognition process employed by many flowering plant species to prevent inbreeding and promote outcrosses.For the Plantaginaceae,Rosa-ceae and Solanaceae,it is now known that S-RNase and S-Iocu F-box(two multiple allelic genes at the S-locus)determine the female and male specificity,respectively,during SI interactions.However,how allelic products of these two genes interact inside pollen tubes to result in specific growth inhibition of self-pollen tubes remains to be investigated.Here,we review all the previously proposed biochemical models and discuss whether their predictions are consistent with all SI phenomena,including competitive jnteraction where SI breaks down in pollen that carries two different pollen 5-alleles.We also discuss these models in Iight of the recent findings of compartmentalization of S-RNases in both incompatible and compatible pollen tubes.Lastly,we summarize the results from our recent biochemical studies of PiSLF(Petunia inflata SLF)and S-RNase.and present a new model for the biochemical mechanism of SI in the Solanaceae.The tenet of this model is that a PiSLF preferentially interacts with its non-self S-RNases in the cytoplasm of a pollen tube to result in the assembly of an E3-like complex,which then mediates ubiquitination and degradation of non-self S-RNases through the ubiquitin-26S proteasome pathway.This model can explain all SI phenomena and,at the same time,has raised new questions for further study.

  4. PERCENTAGE DISTRIBUTION OF ABO AND RHESUS BLOOD GROUPS: A CONCRETE STUDY IN PRAKASAM DISTRICT OF ANDHRA PRADESH

    Bora Prasada

    2015-03-01

    Full Text Available BACKGROUND : ABO and Rh System are the major blood Groups widely distributed in the general population irrespective of their race, ethnicity and geographical distribution. The present study is conducted to identify the distribution of these commonest blood groups prevailing in the Prakasam District of Andhra Pradesh in comparison with related studies in India and abroad. About 2404 samples over a period of 4 months from November 2014 to February 2015, grouped for ABO and Rh - D System(s at Blood Bank, RIMS, Ongole, Prakasam district Andhra Pradesh. METHODS : Ongole is the capital of erstwhile Andhra Pradesh; it is very near to the present proposed capital of Andhra Pradesh. The present study was conducted in human male and female subjects comprising of 2404 attendees to the RIMS Medical College Blood Bank of Prakasam D istrict over a period of 4 months. All the standardized Operating Procedures (SOPs and Quality Control measures are taken for blood group determination including Donor Screening Questionnaire. Subjects with severe medical problems like Cardio respiratory, renal, hepatic and gastrointestinal illnesses were excluded from the study. RESULTS : In the present study in Prakasam district, the most common Blood Group in ABO and Rh system was found to be ‘O’ +ve. Next common in descending order were B +ve, A +ve, AB +ve, O - ve, A - ve, B - ve and AB - ve. In Prakasam District, the commonest Rh Blood type is Rh Positive (94.5%, less frequent is Rh negative (5.5%. In South India Rh Positive is 94.6%, Rh negative is 5.4%. In European Population Rh Positive is 85% and Rh negative is 15%. Of the total 60 people affected by diseases, 4 were affected by HIV, 40 by HBSAG and 16 by HCV. CONCLUSION : It is clearly evident that the most common Blood Group in ABO system is ‘O’. Least common Blood Group is AB. In Rh system D Positive is 94.5% in general population.

  5. FREQUENCY AND DISTRIBUTION OF ABO & RH BLOOD GROUP IN BILASPUR DISTRICT OF CHHATTISGARH STATE : A STUDY FROM MEDICAL COLLEGE HOSPITAL

    Bhanu Pratap; De, Archana; Watsala; Anil K; De, Shipra

    2015-01-01

    BACKGROUND : Approximate 30 blood group systems have discovered and more than 400 erythrocytes antigens are identified. Blood group ABO and Rh are most important among all other blood group systems in transfusion service practices. The frequency of four major blood gr oup s namely A, B, O, AB with Rh Positive and Negative varies in different population of the world and differ also in region and race wise. MATER...

  6. Sample Acquisition and Analytical Chemistry Challenges to Verifying Compliance to Aviators Breathing Oxygen (ABO) Purity Specification

    Graf, John

    2015-01-01

    NASA has been developing and testing two different types of oxygen separation systems. One type of oxygen separation system uses pressure swing technology, the other type uses a solid electrolyte electrochemical oxygen separation cell. Both development systems have been subjected to long term testing, and performance testing under a variety of environmental and operational conditions. Testing these two systems revealed that measuring the product purity of oxygen, and determining if an oxygen separation device meets Aviator's Breathing Oxygen (ABO) specifications is a subtle and sometimes difficult analytical chemistry job. Verifying product purity of cryogenically produced oxygen presents a different set of analytical chemistry challenges. This presentation will describe some of the sample acquisition and analytical chemistry challenges presented by verifying oxygen produced by an oxygen separator - and verifying oxygen produced by cryogenic separation processes. The primary contaminant that causes gas samples to fail to meet ABO requirements is water. The maximum amount of water vapor allowed is 7 ppmv. The principal challenge of verifying oxygen produced by an oxygen separator is that it is produced relatively slowly, and at comparatively low temperatures. A short term failure that occurs for just a few minutes in the course of a 1 week run could cause an entire tank to be rejected. Continuous monitoring of oxygen purity and water vapor could identify problems as soon as they occur. Long term oxygen separator tests were instrumented with an oxygen analyzer and with an hygrometer: a GE Moisture Monitor Series 35. This hygrometer uses an aluminum oxide sensor. The user's manual does not report this, but long term exposure to pure oxygen causes the aluminum oxide sensor head to bias dry. Oxygen product that exceeded the 7 ppm specification was improperly accepted, because the sensor had biased. The bias is permanent - exposure to air does not cause the sensor to

  7. Frequencies and ethnic distribution of ABO and Rh(D) blood groups in Mauritania: results of first nationwide study.

    Hamed, C T; Bollahi, M A; Abdelhamid, I; Med Mahmoud, M A; Ba, B; Ghaber, S; Habti, N; Houmeida, A

    2012-04-01

    There is no data available on the ABO/Rh(D) frequencies in the Mauritanian population. We retrospectively analysed records of a 5-year database that contained ABO/Rh phenotype and ethnic origin of 10 116 volunteers giving blood at the national blood transfusion centre to derive the frequencies of ABO/Rh(D) groups in the Mauritanian population. The two race categories in the country and their sub-ethnic groups: the Moors (whites and black) and the black Africans (Pulhars, Soninkes and Wolof) were included in this study. Globally, group O had the highest frequency (49.10%) followed by A (28.28%), B (18.56%) and AB (4.05%). This order more common in North African populations was found in four of the five ethnic groups composing our population. Allele frequencies were, respectively, 70.20%, 17.74% and 12.04% giving the same order of O > A > B. We observed no significant variation in these frequencies between the different ethnic groups. Rhesus study showed that with a percentage of 94.23% Rh(D) positive is by far the most prevalent, while Rh(D) negative is present only in 5.77% of the total population. This frequency distribution supports the mixed-race composition of the Mauritanian population. PMID:22128837

  8. DISTRIBUTION OF CLASSICAL ABO BLOOD GROUPS AMONG TYPE 2 DIABETES MELLITUS PATIENTS : AN ANALYSIS

    Rama Devi

    2015-04-01

    Full Text Available BACKGROUND: At present Diabetes Mellitus is a global phenomenon with the disease topping the list, comprising of about 32 million cases , India is in the forefront with 30% of the cases . The disease affects multiple organs and is a leading cause of much morbidity and mortality. Since it is a multi - factorial disease a major step would be to identify different associated factors, for an early diagnosis and prompt treatment. The ABO blood groups are often associated with several diseases, with one blood group more often seen with the patients of a particular disease. Our study will help to determine the frequency and distribution of blood groups in correlation with Diabetes Mellitus. MATERIAL & METHODS: This study was conducted in the Gandhi Medical College, Secunderabad, during a two year period. A random study involving every third diabetic patient was chosen and their blood group was determined. A total of 3 00 patients were selected with 150 male and 150 female patients. Another 300 volunteers who were not diabetics were chosen as controls and their blood groups were also determined. A pro - forma was given to both diabetics and controls which included the following variables : 1 . Demographic data 2. Blood grouping 3. Fasting and post prandial blood sugar. Following this, blood groups of both cohorts and controls were determined by antigen antibody agglutination method. Data analysis was do ne after data was entered into excel sheet and double checked for errors using SPSS Software RESULTS: Our a nalysis showed that O group was significantly more among diabetic patients when all patients were compared to control . ² there was a preponderance of blood group O among female diabetics and B among male diabetics. CONCLUSION: ABO blood groups have been determined in 300 diabetic patients and compared with the controls comprising of a series of 300 voluntary blood donors. When the results were analysed on the basis of sex, there was preponderance

  9. Streamflow, Infiltration, and Ground-Water Recharge at Abo Arroyo, New Mexico

    Stewart-Deaker, Amy E.; Stonestrom, David A.; Moore, Stephanie J.

    2007-01-01

    Abo Arroyo, an ephemeral tributary to the Rio Grande, rises in the largest upland catchment on the eastern side of the Middle Rio Grande Basin (MRGB). The 30-kilometer reach of channel between the mountain front and its confluence with the Rio Grande is incised into basin-fill sediments and separated from the regional water table by an unsaturated zone that reaches 120 meters thick. The MRGB portion of the arroyo is dry except for brief flows generated by runoff from the upland catchment. Though brief, ephemeral flows provide a substantial fraction of ground-water recharge in the southeastern portion of the MRGB. Previous estimates of average annual recharge from Abo Arroyo range from 1.3 to 21 million cubic meters. The current study examined the timing, location, and amount of channel infiltration using streamflow data and environmental tracers during a four-year period (water years 1997?2000). A streamflow-gaging station (?gage?) was installed in a bedrock-controlled reach near the catchment outlet to provide high-frequency data on runoff entering the basin. Streamflow at the gage, an approximate bound on potential tributary recharge to the basin, ranged from 0.8 to 15 million cubic meters per year. Storm-generated runoff produced about 98 percent of the flow in the wettest year and 80 percent of the flow in the driest year. Nearly all flows that enter the MRGB arise from monsoonal storms in July through October. A newly developed streambed temperature method indicated the presence and duration of ephemeral flows downstream of the gage. During the monsoon season, abrupt downward shifts in streambed temperatures and suppressed diurnal ranges provided generally clear indications of flow. Streambed temperatures during winter showed that snowmelt is also effective in generating channel infiltration. Controlled infiltration experiments in dry arroyo sediments indicated that most ephemeral flow is lost to seepage before reaching the Rio Grande. Streambed temperature

  10. Helicobacter pylori infection according to ABO blood group among blood donors in Kosovo

    Bukurije Zhubi

    2011-09-01

    Full Text Available Introduction: Numerous studies have reported a high prevalence of Helicobacter pylori infection among healthy and non-healthy persons in different places. The Aim of the study is to investigate the seroprevalence of H. pylori infection among Kosovo’s Blood donor associated with ABO/Rhesus blood group.Methods: 671 blood donors are tested for H. pylori antibodies and results are classifi ed by way of donation, age, gender, blood groups and education level. Serum antibodies are analyzed by Enzyme Linked Fluorescent Assay test for H. pylori IgG with Biomerieux HPY-VIDAS.Results: The frequency of IgG antibody for H. pylori among healthy blood donors is 56.9%, there is not found any difference between voluntary and non-voluntary blood donors (57.4% respectively 56.3%(OR=1.05; 95% CI 0.76 to 1.43; p=0.8. H pylori IgG antibodies positive are detected in 57.0 % ( 126 of 221 of women, compared with 56.9 % ( 256 of 450 of men(OR=0.99; 95% CI 0.72 to 1.38; p=0.96. Serpositive donors are older than seronegative ones (31.9 years, respectively 29.5 years, p=0.02. Mean value of IgG antibody of H. pylori is 3.61 with no significant difference between males and females (3.72 respectively 3.44; p=0.2. The seroprevalence of H. pylori infection is similar among blood groups: O (57.4%, A (56.2%, B (59.6%, AB (51.4%, RhD positive (56.7% and RhD negative (58.3%.Conclusions: The seropositivity of H. pylori is moderately higher in the non voluntary and familiar blood donors among the total Kosovo blood donors. There is not found a significant relationship between infection with H. pylori and ABO/Rhesus blood group among blood donors.

  11. 40 CFR 267.17 - What are the requirements for managing ignitable, reactive, or incompatible wastes?

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false What are the requirements for managing ignitable, reactive, or incompatible wastes? 267.17 Section 267.17 Protection of Environment ENVIRONMENTAL... requirements for managing ignitable, reactive, or incompatible wastes? (a) You must take precautions to...

  12. A preliminary survey into ways of overcoming self-incompatibility in theobroma cacao L

    Varying concentrations of NAA and GA3 (100-500 mg/l) were applied to the base of self-incompatible cocoa flowers with the view to delaying floral abscission and thus lengthening the time needed for the fusion of self-incompatible male and female gametes. NAA above 300 mg/l slightly delayed floral abscission but this did not help overcome the self-incompatibility whilst none of the GA3 concentrations used either delayed floral abscission or promoted self-compatibility. Similar concentrations of both hormones when applied to the stigma before selfing also did not help to overcome the self-incompatibility. Self-incompatibility was partially overcome by treating compatible pollen with 60Gy of gamma rays and mixing it with self-incompatible pollen. Compatible pollen treated at 60Gy and used alone to pollinate self-incompatible flowers resulted in 100 percent flat, non-viable cocoa beans whilst gamma treated pollen mixed with self-incompatible pollen produced about 30 percent fully formed and viable cocoa beans. This appears to suggest that the irradiated compatible pollen is acting as mentor pollen and promoting selfing. (author)

  13. Supervisee Incompatibility and Its Influence on Triadic Supervision: An Examination of Doctoral Student Supervisors' Perspectives

    Hein, Serge F.; Lawson, Gerard; Rodriguez, Christopher P.

    2011-01-01

    A qualitative study was conducted to explore supervisors' experiences of supervisee incompatibility in triadic supervision. In-depth interviews were completed with 9 doctoral student supervisors in a counselor education program, and a whole-text analysis generated 3 categories. Supervisee incompatibility took a wide variety of forms and negatively…

  14. 40 CFR Appendix V to Part 264 - Examples of Potentially Incompatible Waste

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Examples of Potentially Incompatible Waste V Appendix V to Part 264 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... DISPOSAL FACILITIES Pt. 264, App. V Appendix V to Part 264—Examples of Potentially Incompatible Waste...

  15. Within- and between-population variation for Wolbachia-induced reproductive incompatibility in a haplodiploid mite

    F. de Freitas Vala Salvador; A. Weeks; D. Claessen; J.A.J. Breeuwer; M.W. Sabelis

    2002-01-01

    Wolbachia pipientis is a bacterium that induces cytoplasmic incompatibility (CI), the phenomenon in which infected males are reproductively incompatible with uninfected females. CI spreads in a population of hosts because it reduces the fitness of uninfected females relative to infected females. CI

  16. Complex Self-Incompatibility Systems in Ranunculus acris L. and Beta vulgaris L

    Lundqvist, A.; Østerbye, U.; Larsen, K.; Linde-Laursen, Ib

    1973-01-01

    acris, and in Beta vulgaris there are at least four. The observations strongly support the theory of the incompatibility genes being ancient constituents of the breeding systems of the angiosperms. Most probably a complex type of incompatibility control was already present at the presumed common...

  17. Caso de miasis orbitaria

    Lyane Casas Pérez; Jorge G. Martín Martínez; Aime Noa Barrios

    2009-01-01

    Se presenta un caso de un paciente masculino de ochenta y tres años de edad, de raza blanca con antecedentes de la enfermedad del Parkinson con una miasis orbitaria derecha profunda producida por el gusano barrenador de la mosca Cochlimyia hominivorax. Se realizó un reporte de caso con el objetivo de describir el cuadro médico que presentaba el anciano. Presentó abundantes larvas en una zona necrótica en el párpado superior derecho, con fetidez y ninguna sensación en la zona que permitió real...

  18. Seroprevalence of Helicobacter pyloriin school-aged Chinese in Taipei City and relationship between ABO blood groups

    Tzee-Chung Wu; Liang-Kung Chen; Shinn-Jang Hwang

    2003-01-01

    AIM: To explore the seropositive rate of antibodies against H. pylori(anti-HP) in Taipei City and to compare the relationship of ABO blood groups and H. pylori infection.METHODS:In 1993, high school students in Shih-Lin District were randomly selected for blood samplings by their registration number at school. In addition, similar procedures were performed on the well-children clinics of Taipei Veterans General Hospital. Besides, randomly selected sera from the adults who took the physical examination were recruited for evaluation. Informed consents were obtained from all the subjects before blood samplings and parents were simultaneously informed for those who were younger than 18-year-old. Blood tests for anti-HP and ABO blood groupings were performed by enzyme-linked immunosorbent assay.Chi square tests were used for the comparisons between seroprevalence of H. pylori and ABO blood groups.RESULTS: Totally, 685 subjects were recruited (260 children aged 1-14 years, 425 high school students aged 15-18 years)were evaluated, and another 88 adult healthy volunteers were studied as well for comparison. The age-specific seropositive rate of anti-HP was 1.3 % at age 1-5 years,7.7 % at age 6-10 years, and 11.5 % at age 11-14 years.The seroprevalence of H. pylori infection was abruptly increased in young adolescence: 18.6 % at age 15 years,28.1% at age 16 years, 32.4 % at age 17 years and 41.0%at age 18 years, respectively. In the 425 high school students,ABO blood groupings were performed, which disclosed 48.5 % (206/425) of blood group O, 24 % (102/425) of blood group A, 21.8 % (93/425) of blood group B and 5.6 %(24/425) of blood group AB. In comparison of the subjects with blood group O and the other blood groups, no statistical significance could be identified in the seroprevalence of H. pylori(P=0.99).CONCLUSION: The seroprevalence of H. pylori infection in Taipei City in adults is similar to the developed countries,and the abrupt increase of H. pylori during high

  19. A STUDY TO FIND CORRELATION BETWEEN DERMATOGLYPHIC PATTERNS AND ABO BLOOD GROUPS

    Usha Verma

    2015-09-01

    Full Text Available Background: Dermatoglyphics, the study of fingerprints are constant and individualistic. It has been found useful in forensic medicine and identification purpose. It is useful in medical diagnosis of genetically inherited diseases and in detection of crimes. Objectives: The present study was conducted to correlate between digital dermatoglyphics patterns in ABO, Rh blood groups and to evaluate their significance. Methods: A total of 200 first year MBBS students of Pt. B.D. Sharma PGIMS, Rohtak with known blood groups from age group 17-22 yrs were included in the study. Fingerprints were obtained by Ink method. Parameters studied were arches, whorls, loops. Results: Majority of the subjects (43.5% in the study were of blood group A followed by blood group O, A and AB of whom 94.5% were Rh-positive. The general distribution of pattern of finger print showed high frequency (51.87% of loops followed by whorls and arches. Almost same order was noticed in both Rh-positive and Rh-negative individuals or A, B, AB and O blood groups, except blood group O-ve which showed more whorls. Conclusion: There is an association between distribution of finger print pattern and blood groups.

  20. STUDY OF SEX, AGE AND BLOOD GROUPS (ABO, Rh DISTRIBUTIONS IN THALASSEM IA PATIENTS IN IRAN

    DD Farhud; H Sadighi ; M.R. Mohammad Hassani ; A. Samavat; R. Zakizadeh ; Z. Yazdani

    1996-08-01

    Full Text Available Thalassemias, because of climatic, geographic and ecological conditions, are the most common among the genetically endemic in Iran, especially m provinces adjacent to the Caspian Sea and the Persian Gulf. There are over 14,000 cases of thalassemia major reported in Iran. Data, collected by the Iranian Ministry of Health, and analyzed at the Department of Human Genetics, School of Public Health, Tehran University of Medical Sciences, showed relative distribution of 3194 patients referring for the iron chelating drug, disferal, in 24 provinces in Iran. 3304 cases were studied for sex and age groups. Higher percentages and sex ratios were observed in each age group and further clarified as the age increased. 3386 cases were considered for ABO and Rh blood groups. Significant high incidence of group 0 (41.228% was followed by groups A (29.090% and B (23.2 13%, and group AB with the lowest (6.467%. A significant low incidence of Rh negative was also observed (6.852%.

  1. Early plasmapheresis and rituximab for acute humoral rejection after ABO-compatible liver transplantation

    Nassim Kamar; Laurence Lavayssière; Fabrice Muscari; Janick Selves; Céline Guilbeau-Frugier; Isabelle Cardeau; Laure Esposito; Olivier Cointault; Marie Béatrice Nogier; Jean Marie Peron; Philippe Otal; Marylise Fort; Lionel Rostaing

    2009-01-01

    Acute humoral rejection (AHR) is uncommon after ABOcompatible liver transplantation. Herein, we report two cases of AHR treated with plasmapheresis and rituximab in two ABO-compatible liver-transplant patients with preformed anti-human leukocyte antigen donor-specific antibodies. Patient 1 experienced a biopsy-proven AHR at day 10 post-transplant. She was treated by steroid pulses, and OKT3. Because of persisting signs of biopsy-proven AHR at day 26, she was treated by plasmapheresis and rituximab. Liver enzyme levels did not improve, and she died on day 41. Patient 2 experienced a biopsy-proven AHR on day 10 post-transplant. She was treated by steroid pulses, plasmapheresis, and rituximab.Liver enzymes returned to within normal range 18 dafter diagnosis. Liver biopsies, at 3 and 9 mo post-transplant,showed complete resolution of AHR. We conclude that plasmapheresis should be started as soon as AHR is diagnosed, and be associated with a B-cell depleting agent. Rituximab may be considered as a first-line therapy.

  2. ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging

    Toemsak Srikhirin

    2013-09-01

    Full Text Available In this study, readily available antibodies that are used in standard agglutination tests were evaluated for their use in ABO blood typing by a surface plasmon resonance imaging (SPR imaging technique. Five groups of antibodies, including mixed clones of anti-A, anti-B, and anti-AB, and single clones of anti-A and anti-B, were used to construct the five-line detection arrays using a multichannel flow cell in the SPR imager. The red blood cell (RBC samples were applied to a multichannel flow cell that was orthogonal to the detection line arrays for blood group typing. We found that the blood samples were correctly grouped in less than 12 min by the SPR imaging technique, and the results were consistent with those of the standard agglutination technique for all 60 samples. We found that mixed clones of antibodies provided 33%–68% greater change in the SPR signal than the single-clone antibodies. Applying the SPR imaging technique using readily available antibodies may reduce the costs of the antibodies, shorten the measurement time, and increase the throughput.

  3. The prevalence of transfusion transmitted infections in ABO blood groups and Rh type system

    Jitendra Singh Nigam

    2014-12-01

    Full Text Available Screening of blood and blood products is important to reduce the risk of transfusion transmitted infections (TTIs. The transfusion of unscreened or inadequately screened blood and blood products are the major source of TTIs. The aim of this paper is to find out the prevalence of TTIs in ABO blood groups and Rh type system. A total of 4128 blood donors were screened from January 2010 to April 2014. Serological tests were performed for hepatitis B surface antigen (HBsAg, anti hepatitis C virus (Anti-HCV, anti HIV-1 and 2, venereal disease research laboratory test (VDRL and malaria parasite (MP antigen. In seroreactive donors, HBsAg, Anti-HCV, VDRL, MP antigen and anti HIV were positive in 40 cases, 26 cases, 19 cases, 6 cases and 2 cases, respectively. Highest percentage of HBsAg, Anti HCV, VDRL, MP antigen and anti HIV was observed in blood group A negative (2/50, O negative (1/66, B negative (1/91, AB positive (2/377 blood group respectively. In the present study, the total number of Rh-negative donors is lower when compared to Rh-positive blood donors, but Rh-negative blood donors show higher percentages of seroreactivity for TTIs. Larger scale studies at molecular level are required to improve the knowledge of this aspect.

  4. ABO blood grouping from hard and soft tissues of teeth by modified absorption-elution technique

    B K Ramnarayan

    2013-01-01

    Full Text Available Background: Teeth have always been known as stable tissue that can be preserved both physically and chemically for long periods of time. Blood group substances have been known to be present in both the hard and soft tissues of the teeth. Objectives: This study aimed at detection of ABO blood group substances from soft and hard tissues of teeth and also to evaluate the reliability of teeth stored for a relatively long period as a source of blood group substances by absorption-elution technique with some modifications. Results: Blood group obtained from the teeth was compared with those obtained from the blood sample. Pulp showed a very large correlation in both fresh and long-standing teeth though it decreased slightly in the latter. Hard tissue showed a large correlation in both the groups indicating that hard tissue is quite reliable to detect blood group and that there is no much difference in the reliability in both the groups. However, combining pulp and hard tissue, correlation is moderate. Correlation of blood grouping with the age, sex, and jaw distribution was carried out. Conclusion: Blood group identification from hard and soft tissues of teeth aids in the identification of an individual.

  5. Genetic incompatibility dampens hybrid fertility more than hybrid viability: yeast as a case study.

    Meibo Xu

    Full Text Available Genetic incompatibility is believed to be the major cause of postzygotic reproductive isolation. Despite huge efforts seeking for speciation-related incompatibilities in the past several decades, a general understanding of how genetic incompatibility evolves in affecting hybrid fitness is not available, primarily due to the fact that the number of known incompatibilities is small. Instead of further mapping specific incompatible genes, in this paper we aimed to know the overall effects of incompatibility on fertility and viability, the two aspects of fitness, by examining 89 gametes produced by yeast S. cerevisiae-S. paradoxus F1 hybrids. Homozygous F2 hybrids formed by autodiploidization of F1 gametes were subject to tests for growth rate and sporulation efficiency. We observed much stronger defects in sporulation than in clonal growth for every single F2 hybrid strain, indicating that genetic incompatibility affects hybrid fertility more than hybrid viability in yeast. We related this finding in part to the fast-evolving nature of meiosis-related genes, and proposed that the generally low expression levels of these genes might be a cause of the observation.

  6. A Method for Prediction of Graft Incompatibility in Sweet Cherry

    S. Filiz GUCLU

    2012-05-01

    Full Text Available This study was carried out on 1-year old trees of ‘0900 Ziraat’ variety grafted onto ‘Kuş kirazi’, ‘Kara idris’, ‘Sari idris’, ‘MaxMa 14’, ‘MaxMa 60’ and ‘Gisela 5’ in order to determine their compatibility. For this purpose, total peroxidase activity was determined by spectrophotomery assay. Taking ground tissue samples were planned three times as before the grafting (beginning, and then 8 and 12 months after grafting. Total peroxidase activities of rootstocks before grafting varied between 10.80 ΔAg.min. (‘Kuş kirazi’ and 7.83 ΔAg.min. (‘Kara idris’ and were found to be statistically important. The peroxidase activity of ‘0900 Ziraat’ was 11.07ΔAg.min. and the closest value occurred in Prunus avium rootstock. The most different values occurred in rootstocks of ‘Kara idris’ and ‘Gisela 5’. The results showed that peroxidase activity increased in rootstock and graft scion. This increase had higher values in heterogenetic combinations especially in ‘0900 Ziraat’/‘Gisela 5’ and ‘0900 Ziraat’/‘Kara idris’ grafts. Peroxidase activity was decreased at the 12th month for all combinations. The highest value was obtained from ‘0900 Ziraat’/‘MaxMa 14’ combinaion with 29.17 ΔAg.min. while lowest one was ‘0900 Ziraat’/‘Kuş kirazi’ with 17.39. The findings showed that peroxidase activity could be used as a parameter in early determination of graft incompatibility.

  7. Arrow physicians: are economics and medicine philosophically incompatible?

    Tsang, Sandro

    2015-06-01

    Economics is en route to its further expansion in medicine, but many in the medical community remain unconvinced that its impact will be positive. Thus, a philosophical enquiry into the compatibility of economics and medicine is necessary to resolve the disagreements. The fundamental mission of medicine obliges physicians to practise science and compassion to serve the patient's best interests. Conventional (neoclassical) economics assumes that individuals are self-interested and that competitive markets will emerge optimal states. Economics is seemingly incompatible with the emphasis of putting patients' interests first. This idea is refuted by Professor Kenneth Arrow's health economics seminal paper. Arrow emphasizes that medical practice involves agency, knowledge, trust and professionalism, and physician-patient relation critically affects care quality. The term Arrow Physician is used to mean a humanistic carer who has a concern for the patient and acts on the best available evidence with health equity in mind. To make this practice sustainable, implementing appropriate motivations, constitutions and institutions to enable altruistic agency is critical. There is substantial evidence that polycentric governance can encourage building trust and reciprocity, so as to avoid depletion of communal resources. This paper proposes building trusting institutions through granting altruistic physicians adequate autonomy to direct resources based on patients' technical needs. It also summarizes the philosophy bases of medicine and economics. It, therefore, contributes to developing a shared language to facilitate intellectual dialogues, and will encourage trans-disciplinary research into medical practice. This should lead to medicine being reoriented to care for whole persons again. PMID:25850973

  8. Histo-blood group ABO antigen in oral potentially malignant lesions and squamous cell carcinoma--genotypic and phenotypic characterization

    Gao, Shan; Bennett, Erik Paul; Reibel, Jesper;

    2004-01-01

    Loss of histo-blood group A/B antigens is frequent in oral cancer. It is unclear whether this alteration is due to loss of the chromosomal region encoding the genes. The aim was to investigate genotypic alterations in the ABO locus in oral potentially malignant lesions and carcinomas. Seventy...... and 3/24 cases with mild and moderate dysplasia by genotyping analysis. O allele loss was found in 10 cases involving all four groups. In patients with heterozygous genotypes, A/B allelic loss by genotyping analysis was always followed by loss of A/B antigen expression by IHC staining. Loss of A...

  9. Linkage disequilibrium between incompatibility locus region genes in the plant Arabidopsis lyrata

    Hagenblad, Jenny; Bechsgaard, Jesper Smærup; Charlesworth, Deborah

    2006-01-01

    We have studied diversity in Arabidopsis lyrata of sequences orthologous to the ARK3 gene of A. thaliana. Our main goal was to test for recombination in the S-locus region. In A. thaliana, the single-copy ARK3 gene is closely linked to the non-functional copies of the self-incompatibility loci, and...... the ortholog in A. lyrata (a self-incompatible species) is in the homologous genome region and is known as Aly8. It is thus of interest to test whether Aly8 sequence diversity is elevated due to close linkage to the highly polymorphic incompatibility locus, as is theoretically predicted. However, Aly8...... linked to the incompatibility locus, one being a pseudogene. We determined the phase of multiple haplotypes in families of plants from Icelandic and other populations. Different Aly8 sequence types are associated with different SRK alleles, while haplotypes with the same SRK sequences tend to have the...

  10. Spectrum and Outcome Analysis of Marked Neonatal Hyperbilirubinemia with Blood Group Incompatibility

    Yi-Hao Weng

    2009-08-01

    Full Text Available Background: Blood group mismatch between a mother and newborn carries a substantialrisk for neonatal hyperbilirubinemia and kernicterus. In the current study, weinvestigate the spectrum and outcome of marked neonatal hyperbilirubinemiawith blood group incompatibility.Methods: We retrospectively assessed a cohort of 413 neonates with peak total serumbilirubin (TSB values ≥ 20 mg/dL between 1995 and 2007. Those with agestational age 7%, a hemoglobin value < 13 g/dL and apeak TSB at age < 3 days old than the controls (p < 0.01. Furthermore, kernicterus was more common in neonates with blood group incompatibility(9.8% than in the controls (0.0% (p < 0.01.Conclusions: This survey depicts the clinical profiles of babies with marked neonatalhyperbilirubinemia with blood group incompatibility. Neonates with bloodgroup incompatibility often develop early-onset, hemolysis-mediated hyperbilirubinemia. Our findings show they are at great risk of kernicterus.

  11. Expression of self-incompatibility ribonucleases of Antirrhinum in Escherichia coli

    2000-01-01

    Self-incompatibility is an intraspecific reproductive barrier to prevent self-fertilization in the flowering plants.In many species,self-incompatibility is controlled by a single S locus with multiple alleles.So far,the only gene known in the S locus of the Solanaceae,Scrophulariaceae and Rosaceae encodes a class of ribonucleases,called self-incompatibility ribonucleases (S RNases),which have been shown to mediate stylar expression of self-incompatible reaction.As the first step to investigate their three-dimensional structure,we successfully expressed three biologically active S RNases of Antirrihnum (S2,S4 and S5) in Escherichia coli (E.coli).Their functional expressions caused no detrimental effect on host bacteria growth and provided a basis for a large scale preparation of S RNase proteins.Possible reasons for non-lethality of S RNases on E.coli are discussed.

  12. Against the Quantitative-Qualitative Incompatibility Thesis or Dogmas Die Hard.

    Howe, Kenneth R.

    1988-01-01

    Employs a pragmatic philosophical perspective to argue that there is no incompatibility at either the level of practice or that of epistemology between qualitative and quantitative methods of educational research. (FMW)

  13. Tuberculose ocular: relato de casos

    Costa Daniella Socci da; Silva Rodrigo Tavares Schueler e; Klejnberg Tatina; Japiassu Ricardo; Turchetti Remo; Moraes Jr. Haroldo Vieira de

    2003-01-01

    Os autores relatam dois casos de tuberculose ocular presumida com comprometimento coroidiano. No primeiro caso, um paciente de 43 anos, HIV positivo, com tuberculose pulmonar miliar e tuberculoma cerebral, apresenta um granuloma coroidiano único na região macular do olho esquerdo. No segundo caso, uma paciente de 12 anos, HIV negativa, com tuberculose pulmonar apresenta coroidite multifocal e comprometimento do segmento anterior do olho esquerdo. Ambos os pacientes evoluíram favoravelmente co...

  14. Multilocus PCR Assays Elucidate Vegetative Incompatibility Gene Profiles of Cryphonectria parasitica in the United States

    Short, Dylan P. G.; Double, Mark; Nuss, Donald L.; Stauder, Cameron M.; MACDONALD, WILLIAM; Kasson, Matthew T.

    2015-01-01

    Chestnut blight is a devastating disease of Castanea spp. Mycoviruses that reduce virulence (hypovirulence) of the causative agent, Cryphonectria parasitica, can be used to manage chestnut blight. However, vegetative incompatibility (vic) barriers that restrict anastomosis-mediated virus transmission hamper hypovirulence efficacy. In order to effectively determine the vegetative incompatibility genetic structure of C. parasitica field populations, we have designed PCR primer sets that selecti...

  15. Evolution Controversy: A Phenomenon Prompted by the Incompatibility between Science and Religious Beliefs

    Paz-y-Miño-C, Guillermo; Espinosa, Avelina

    2015-01-01

    The incompatibility between science and the belief in supernatural causation helps us understand why people do not accept evolution. Belief disrupts, distorts, delays, or stops (3Ds + S) the acceptance of scientific evidence. Here we examine the evolution controversy under three predictions of the incompatibility hypothesis. First, chronological-conflict-and-accommodation, which explains the historical re-emergence of antagonism between evolution and religion when advances in science continue...

  16. Contrasting levels of variability between cytoplasmic genomes and incompatibility types in the mosquito Culex pipiens.

    Guillemaud, T; Pasteur, N; Rousset, F

    1997-02-22

    Reproductive incompatibilities called cytoplasmic incompatibilities are known to affect a large number of arthropod species and are mediated by Wolbachia, a maternally transmitted microorganism. The crossing relationships between strains of potential hosts define their incompatibility types and it is generally assumed that differences between strains of Wolbachia induce different crossing types. Among all the described host species, the mosquito, Culex pipiens, displays the greatest variability of cytoplasmic incompatibility crossing types. We analysed mitochondrial and bacterial DNA variability in Culex pipiens in order to investigate some possible causes of incompatibility crossing type variability. We sequenced fragments of the ftsZ gene, and the A + T-rich control region of the mtDNA. We also sequenced the second subunit of the mitochondrial cytochrome oxidase (COII) gene, in Culex pipiens and a closely related species, C. torrentium, in order to verify the usefulness of the A + T-rich region for the present purposes. No variability was found in the Wolbachia ftsZ gene fragment, and very limited variation of the mitochondrial marker whatever the compatibility type or the origin of the host. A low variability was found in the A + T-rich region and comparison of divergence of the A + T-rich region and COII gene between C. pipiens and C. torrentium did not reveal any special constraints affecting this region. In contrast to observations in other host species, variability of incompatibility crossing types is not due to multiple infections by distantly related Wolbachia strains. PMID:9061971

  17. Expression and inheritance of sporophytic self-incompatibility in synthetic allohexaploid Senecio cambrensis (Asteraceae).

    Brennan, Adrian C; Hiscock, Simon J

    2010-04-01

    Allopolyploid speciation is common in plants and is frequently associated with shifts from outcrossing, for example self-incompatibility, to inbreeding (i.e. selfing). Senecio cambrensis is a recently evolved allohexaploid species that formed following hybridization between diploid self-incompatible S. squalidus and tetraploid self-compatible S. vulgaris. Studies of reproduction in wild populations of S. cambrensis have concluded that it is self-compatible. Here, we investigated self-compatibility in synthetic lines of S. cambrensis generated via hybridization and colchicine-induced polyploidization and wild S. cambrensis using controlled crossing experiments. Synthetic F(1)S. cambrensis individuals were all self-compatible but, in F(2) and later generations, self-incompatible individuals were identified at frequencies of 6.7-9.2%. Self-incompatibility was also detected in wild sampled individuals at a frequency of 12.2%. The mechanism and genetics of self-incompatibility were tested in synthetic S. cambrensis and found to be similar to those of its paternal parent S. squalidus (i.e. sporophytic). These results show, for the first time, that functional sporophytic self-incompatibility can be inherited and expressed in allopolyploids as early as the second (F(2)) generation. Wild S. cambrensis should therefore be considered as possessing a mixed mating system with the potential for evolution towards either inbreeding or outcrossing. PMID:19895670

  18. Posttreatment and retention outcomes with and without periodontally accelerated osteogenic orthodontics assessed using ABO objective grading system

    Donald J Ferguson

    2016-01-01

    Full Text Available Background: The posttreatment and retention outcomes following nonextraction orthodontic therapy, with and without corticotomy, were assessed using the American Board of Orthodontists objective grading system (OGS. Purpose: The purpose was to determine if the course of retention was any different following alveolar decortication and augmentation bone grafting, i.e., periodontally accelerated osteogenic orthodontics (PAOO. Materials and Methods: Study casts and panoramic radiographs of patients with and without PAOO (28 subjects each were selected on the basis of the following: (1 comprehensive nonextraction orthodontic treatment using straight wire edgewise appliances for Class I crowding, (2 availability of immediate posttreatment records and retention records at least 1 year post de-bracketing, and (3 use of Hawley removable retainers with similar wearing instructions. Results: Independent and paired t-test statistical testing revealed the following: (1 Posttreatment orthodontic outcomes were the same, with or without corticotomy. (2 During retention, 5 of 8 ABO grading criteria improved for the sample without corticotomy, and 6 of 8 ABO grading criteria improved for the group with corticotomy. (3 Retention outcome scores were lower (better for alignment and marginal ridges in the corticotomy-facilitated group. (4 The total score was significantly lower (better for the corticotomy group at retention and the increment of total score change decreased (improved significantly more during retention following corticotomy. Conclusions: The retention phase was more favorable following corticotomy because the amount of OGS total score change demonstrated a significantly improved retention outcome following PAOO therapy.

  19. Screening for neonatal hyperbilirubinaemia and ABO alloimmunization at the time of testing for phenylketonuria and congenital hypothyreosis.

    Meberg, A; Johansen, K B

    1998-12-01

    In a population-based study including 2463 infants, serum bilirubin measurements were added to the neonatal screening programme for phenylketonuria and congenital hypothyreosis. This screening programme detected 11/17 (65%) of infants with serum bilirubin levels >350 micromol 1(-1), of whom 7 (3 per 1000) were readmitted from home (6 treated with phototherapy). A total of 139 infants (5.6%) received phototherapy. Maternal blood type O occurred significantly more often in term infants treated (30/54; 55.6%) compared with preterm infants treated (32/85; 37.6%) and with blood type O occurrence in the total population of mothers (906/2426; 37.3%) (p constellations mother O/infant A or B showed a sensitivity of 64%, specificity 65%, positive predictive value 12% and a negative predictive value of 96% for the requirement of phototherapy for the whole material. Exchange transfusion was not required in any of the infants. No infant developed bilirubin encephalopathy (kemicterus). Adding bilirubin to a neonatal screening programme detects some cases with unexpectedly high bilirubin levels in need of intervention. Routine ABO blood typing of pregnant women, ABO cord blood typing and Coombs' test in infants of mothers with blood type O cannot be recommended because of low positive predictive value for the requirement of intervention (phototherapy) by these tests. PMID:9894828

  20. ABO blood types and cancer risk—A cohort study of 339,432 subjects in Taiwan

    Sun, Wenjie; Wen, Chi-Pang; Lin, Jie; Wen, Christopher; Pu, Xia; Huang, Maosheng; Tsai, Min Kuang; Tsao, Chwen Keng; Wu, Xifeng; Chow, Wong-Ho

    2016-01-01

    Background The associations of laboratory-based ABO phenotypes with cancer risks and mortality have not been systematically determined. Methods The study subjects were 339,432 healthy individuals with laboratory-based blood types from a Taiwan cohort. Results Compared to blood type O, blood type A was significantly associated with an elevated risk of stomach cancer incidence (Hazard Ratio [HR], 1.38 [95% CI, 1.11–1.72]) and mortality (HR, 1.38 [95% CI, 1.02–1.86]) compared with blood type O, after adjusting for age, sex, education, smoking, alcohol drinking, physical activity, and body mass index. Non-O blood types were associated with an elevated risk of pancreatic cancer, with blood type B reaching statistical significance for incidence (HR, 1.59 [95% CI, 1.02–2.48]) and mortality (HR, 1.63 [95% CI, 1.02–2.60]). In contrast, kidney cancer risk was inversely associated with blood type AB (HR, 0.41 [95% CI, 0.18–0.93]) compared to type O. Conclusion Cancer risks vary in people with different ABO blood types, with elevated risks of stomach cancer associated with blood type A and pancreatic cancer associated with non-O blood types (A, B, and AB). PMID:25600007

  1. A STUDY OF DISTRIBUTION OF ABO AND RH BLOOD GROUPS SYSTEM AMONG BLOOD DONORS AT A TERTIARY CARE HOSPITAL

    Rajesh Kumar

    2015-02-01

    Full Text Available Up till now about 400red cells antigen have been identified. The majority are inherited by Mendelian fashion. The ABO and Rh blood group system was first to be identified and is most important for blood transfusion purposes. OBJECTIVE: This study was conducted to determine the frequency of ABO and Rhesus (Rh blood groups in a tertiary care teaching hospital in India. MATERIALS AND METHODS: A retrospective data based study was conducted at blood bank , Chirayu Medical College and Hospital, Bhopal, Madhya Pradesh, India over a period of four years. RESULTS: Study includes a record of 3188 (28.54% voluntary and 7982 (71.46% replacement donors attending blood bank from February 2011 to January 2015. Out of 11170, 10723(95.998% were male and 447(4.002% female donors. The most common blood group was found to be B in 4013 (35.927% donors followed by O in 3462 (30.994% donors , an in 2516 (22.524% donors and AB in 1179 (10.555% donors. Out of these, 10659(95.425% donors were Rh - positive while 511 ( 4.575 % were Rh - negative.

  2. Relationship between ABO blood groups and malaria with clinical outcome in rural area of South India

    Gayathri B.N.

    2013-05-01

    Full Text Available Background A number of studies have shown that susceptibility to several infectious diseases is related to the patient’s blood group. Although the relationship between blood group and susceptibility to malaria has been studied by several researchers, the results have been contradictory. Since malaria has re-emerged as a major problem in India during the past few years, it would be useful to know whether there is any relationship between blood group and infection. Objectives The study will be undertaken to correlate the blood groups and clinical presentations in malaria patients and to understand the differential host susceptibility in malaria. Method Over a period of 4 years malaria positive samples identified by peripheral smear (thin and thick smears will be evaluated in this study. Haemoglobin, total leucocyte count, differential leucocyte count and platelet count of each patient done on an automated cell counter will be retrieved from the data. Blood group was determined by forward and reverse method. The demographic details of the patients and clinical details were obtained from case records of the patients. Malarial species and the severity of clinical course were correlated with blood groups Results A total of 205 patients were included in the study, of which 123 cases were positive for plasmodium falciparum and 78 cases were positive for P. vivax infection and 4 patients had mixed infection. The results of blood groups showed 33 -‘A’ group, 84 -‘B’ group, 70 -‘O’ group and 18 were ‘AB’ group. When the clinical courses between different groups were compared using the following parameters for severe infection- a parasitic load of > 10/1000 RBCs, severe anemia with haemoglobin 101o F and the other organ involvement, it was observed that there was no significant relationship between ABO blood group and malaria in our population, this could be due to various demographic reasons. Conclusions The present study indicate that

  3. Erythrocyte-bound apolipoprotein B in relation to atherosclerosis, serum lipids and ABO blood group.

    Boudewijn Klop

    Full Text Available INTRODUCTION: Erythrocytes carry apolipoprotein B on their membrane, but the determining factors of erythrocyte-bound apolipoprotein B (ery-apoB are unknown. We aimed to explore the determinants of ery-apoB to gain more insight into potential mechanisms. METHODS: Subjects with and without CVD were included (N = 398. Ery-apoB was measured on fresh whole blood samples using flow cytometry. Subjects with ery-apoB levels ≤ 0.20 a.u. were considered deficient. Carotid intima media thickness (CIMT was determined as a measure of (subclinical atherosclerosis. RESULTS: Mean ery-apoB value was 23.2% lower in subjects with increased CIMT (0.80 ± 0.09 mm, N = 140 compared to subjects with a normal CIMT (0.57 ± 0.08 mm, N = 258 (P = 0.007, adjusted P<0.001. CIMT and ery-apoB were inversely correlated (Spearman's r: -0.116, P = 0.021. A total of 55 subjects (13.6% were considered ery-apoB deficient, which was associated with a medical history of CVD (OR: 1.86, 95% CI 1.04-3.33; adjusted OR: 1.55; 95% CI 0.85-2.82. Discontinuation of statins in 54 subjects did not influence ery-apoB values despite a 58.4% increase in serum apolipoprotein B. Subjects with blood group O had significantly higher ery-apoB values (1.56 ± 0.94 a.u. when compared to subjects with blood group A (0.89 ± 1.15 a.u, blood group B (0.73 ± 0.1.12 a.u. or blood group AB (0.69 ± 0.69 a.u. (P-ANOVA = 0.002. CONCLUSION: Absence or very low values of ery-apoB are associated with clinical and subclinical atherosclerosis. While serum apolipoprotein B is not associated with ery-apoB, the ABO blood group seems to be a significant determinant.

  4. Whole genome association mapping by incompatibilities and local perfect phylogenies

    Besenbacher Søren

    2006-10-01

    Full Text Available Abstract Background With current technology, vast amounts of data can be cheaply and efficiently produced in association studies, and to prevent data analysis to become the bottleneck of studies, fast and efficient analysis methods that scale to such data set sizes must be developed. Results We present a fast method for accurate localisation of disease causing variants in high density case-control association mapping experiments with large numbers of cases and controls. The method searches for significant clustering of case chromosomes in the "perfect" phylogenetic tree defined by the largest region around each marker that is compatible with a single phylogenetic tree. This perfect phylogenetic tree is treated as a decision tree for determining disease status, and scored by its accuracy as a decision tree. The rationale for this is that the perfect phylogeny near a disease affecting mutation should provide more information about the affected/unaffected classification than random trees. If regions of compatibility contain few markers, due to e.g. large marker spacing, the algorithm can allow the inclusion of incompatibility markers in order to enlarge the regions prior to estimating their phylogeny. Haplotype data and phased genotype data can be analysed. The power and efficiency of the method is investigated on 1 simulated genotype data under different models of disease determination 2 artificial data sets created from the HapMap ressource, and 3 data sets used for testing of other methods in order to compare with these. Our method has the same accuracy as single marker association (SMA in the simplest case of a single disease causing mutation and a constant recombination rate. However, when it comes to more complex scenarios of mutation heterogeneity and more complex haplotype structure such as found in the HapMap data our method outperforms SMA as well as other fast, data mining approaches such as HapMiner and Haplotype Pattern Mining (HPM

  5. Tres casos de odontomas

    Salvador Javier Santos Medina

    2015-03-01

    Full Text Available Los odontomas son los tumores odontogénicos más comunes, casi siempre se diagnostican accidentalmente, en exámenes radiográficos; pueden sospecharse al retardar el brote dentario o producir maloclusiones. Se clasifican en odontomas compuestos y complejos, ambos constituidos por estructuras dentales como: esmalte, dentina, cemento y tejido pulpar. Su eliminación quirúrgica y la biopsia es el tratamiento de elección. El propósito de este trabajo es reportar tres casos de pacientes atendidos en el Hospital Pediátrico de Las Tunas, todos remitidos previamente por el ortodoncista. El primero, tipo compuesto, en un escolar de 9 años de edad, masculino, asociado a un 31 retenido; el segundo caso se trata de un gran odontoma complejo en una escolar de 11 años de edad, femenina, que produjo interferencia en el brote del 11 y el tercero compuesto; en un escolar de 10 años, masculino, en la línea media entre 11 y 21, con producción de diastema y brote del 12 por palatino. De ellos uno fue descubierto por un examen radiográfico de rutina y los otros dos sospechados por la maloclusión secundaria; todos se trataron quirúrgicamente. Estos casos demuestran la frecuencia de estos tumores en la cavidad bucal y la importancia de la realización de exámenes clínicos y radiográficos para su detección y tratamiento precoz, así como la importancia de la interrelación entre el ortodoncista y el maxilofacial en la resolución satisfactoria de esta entidad

  6. Mexcode (caso de estudio)

    Gabriel Arturo García Gómez; Holmer José Reyes Sotelo

    2000-01-01

    DESCRIPCION: El caso plantea la reactivación de un proyecto que se presentó en el año 1995 como alternativa para el desarrollo del Centro del Valle y mejora de la Eficiencia en el manejo del Comercio Exterior de la Cuenca Pacífica. El Proyecto se revivió a raíz de la necesidad de la Compañía patrocinadora de mejorar sus Flujos de Caja proyectados y la reciente autorización para su funcionamiento. El proyecto es el popularmente denominado "Puerto Seco" de la ciudad de Guadalajara de Buga, inte...

  7. Tres casos de odontomas

    Salvador Javier Santos Medina; Haideé Tamara Díaz Méndez; Giselle Ávila Chiong

    2015-01-01

    Los odontomas son los tumores odontogénicos más comunes, casi siempre se diagnostican accidentalmente, en exámenes radiográficos; pueden sospecharse al retardar el brote dentario o producir maloclusiones. Se clasifican en odontomas compuestos y complejos, ambos constituidos por estructuras dentales como: esmalte, dentina, cemento y tejido pulpar. Su eliminación quirúrgica y la biopsia es el tratamiento de elección. El propósito de este trabajo es reportar tres casos de pacientes atendidos en ...

  8. um estudo de caso

    Costa, Cátia Filipa Pereira da

    2011-01-01

    Dissertação apresentada à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Psicologia Jurídica As situações de abuso sexual de crianças nas quais o perpetrador pertence ao sexo feminino obtiveram ao longo dos últimos anos um acrescido reconhecimento por parte da comunidade científica, evidenciado pelo significativo incremento das investigações no âmbito desta temática consistindo na sua maioria estudos de caso. Um conjunto de particularidades encont...

  9. Functional divergence caused by ancient positive selection of a Drosophila hybrid incompatibility locus.

    Daniel A Barbash

    2004-06-01

    Full Text Available Interspecific hybrid lethality and sterility are a consequence of divergent evolution between species and serve to maintain the discrete identities of species. The evolution of hybrid incompatibilities has been described in widely accepted models by Dobzhansky and Muller where lineage-specific functional divergence is the essential characteristic of hybrid incompatibility genes. Experimentally tractable models are required to identify and test candidate hybrid incompatibility genes. Several Drosophila melanogaster genes involved in hybrid incompatibility have been identified but none has yet been shown to have functionally diverged in accordance with the Dobzhansky-Muller model. By introducing transgenic copies of the X-linked Hybrid male rescue (Hmr gene into D. melanogaster from its sibling species D. simulans and D. mauritiana, we demonstrate that Hmr has functionally diverged to cause F1 hybrid incompatibility between these species. Consistent with the Dobzhansky-Muller model, we find that Hmr has diverged extensively in the D. melanogaster lineage, but we also find extensive divergence in the sibling-species lineage. Together, these findings implicate over 13% of the amino acids encoded by Hmr as candidates for causing hybrid incompatibility. The exceptional level of divergence at Hmr cannot be explained by neutral processes because we use phylogenetic methods and population genetic analyses to show that the elevated amino-acid divergence in both lineages is due to positive selection in the distant past-at least one million generations ago. Our findings suggest that multiple substitutions driven by natural selection may be a general phenomenon required to generate hybrid incompatibility alleles.

  10. Variation at ABO histo-blood group and FUT loci and diffuse and intestinal gastric cancer risk in a European population

    Duell, Eric J; Bonet, Catalina; Muñoz, Xavier;

    2015-01-01

    to GC risk in a European population. The authors conducted an investigation of 32 variants at ABO and FUT1-7 loci and GC risk in a case-control study of 365 cases and 1,284 controls nested within the EPIC cohort (the EPIC-Eurgast study). Four variants (including rs505922) in ABO, and allelic blood group...... A (AO+AA, odds ratio=1.84, 95%CI=1.20-2.80) were associated with diffuse-type GC; however, conditional models with other ABO variants indicated that the associations were largely due to allelic blood group A. One variant in FUT5 was also associated with diffuse-type GC, and four variants (and haplotypes...... the role of ABO and FUT alleles in H. pylori infection and subtype-specific gastric carcinogenesis. What's New? Blood type A indicates a higher risk of gastric cancer, but why? This study examined the relationship between blood group genes and cancer. The authors investigated 32 variants among not only...

  11. Comparison of ABO and Rh-D Blood Group Systems between the Garo Tribal People of Mymensingh and General People of Dhaka City

    Ruhul Amin

    2011-01-01

    Full Text Available Background: The distribution pattern of ABO and Rh-D blood group in our country including the tribal people is not fully established as elaborated and large scale studies have not been carried out on it. Therefore this study was designed to observe the distribution pattern of ABO and Rh-D blood groups among the Garo tribes of Mymensingh and general people of Dhaka city. Objectives: To determine and to compare the distribution pattern of ABO and Rh-D blood groups among the Garo tribal people of Mymensingh and general people of Dhaka city and to compare this distribution between this two groups. Materials and Methods: This observational study was conducted in the Department of Physiology, Dhaka Medical College, Dhaka from July 2008 to June 2009. After proper ethical consideration total 900 Garo people of Mymensingh and 784 general people of Dhaka city were included in this study. The Garo localities and the general people of Dhaka city were selected by systematic random sampling. ABO and Rh-D blood groups were determined by the antigen antibody agglutination test of slide method. Chi square statistical analyses were done to compare the results of ABO blood group systems between the Garo people and general people of Dhaka city. Results: This study revealed that there are significant variations in the distribution of ABO and Rh-D blood groups between the Garo tribal people of Mymensingh and the general people of Dhaka city. In this study it was observed that blood group ‘A’ was apparently predominant in Garo population, while blood group ‘B’ was predominant in general population (p<0.001, blood group ‘AB’ and ‘O’ were almost similar in both groups. Rh typing of the participants reveals that majorities of both groups were Rh positive. Rh negative persons are rare in both populations, but it is extremely rare in the Garo population (0.9%. Conclusion: From the findings of the present study it can be concluded that distribution of ABO

  12. Carlo Caso (1940 - 2007)

    Leonardo Rossi

    Carlo Caso (1940 - 2007) Our friend and colleague Carlo Caso passed away on July 7th, after several months of courageous fight against cancer. Carlo spent most of his scientific career at CERN, taking an active part in the experimental programme of the laboratory. His long and fruitful involvement in particle physics started in the sixties, in the Genoa group led by G. Tomasini. He then made several experiments using the CERN liquid hydrogen bubble chambers -first the 2000HBC and later BEBC- to study various facets of the production and decay of meson and baryon resonances. He later made his own group and joined the NA27 Collaboration to exploit the EHS Spectrometer with a rapid cycling bubble chamber as vertex detector. Amongst their many achievements, they were the first to measure, with excellent precision, the lifetime of the charmed D mesons. At the start of the LEP era, Carlo and his group moved to the DELPHI experiment, participating in the construction and running of the HPC electromagnetic c...

  13. ABO (histo) blood group phenotype development and human reproduction as they relate to ancestral IgM formation: A hypothesis.

    Arend, Peter

    2016-01-01

    The formation of a histo (blood) group) ABO phenotype and the exclusion of an autoreactive IgM or isoagglutinin activity arise apparently in identical glycosylation of complementary domains on cell surfaces and plasma proteins. The fundamental O-glycan emptiness of the circulating IgM, which during the neonatal amino acid sequencing of the variable regions is exerting germline-specific O-GalNAc glycan-reactive serine/threonine residues that in the plasma of the adult human blood group O individuals apparently remain associated with the open glycosidic sites on the ABOH convertible red cell surface, must raise suggestions on a transient expression of developmental glycans, which have been "lost" over the course of maturation. In fact, while the mammalian non-somatic, embryogenic stem cell (ESC)- germ cell (GC) transformation is characterized by a transient and genetically as-yet-undefined trans-species-functional O-GalNAc glycan expression, in the C57BL/10 mouse such expression was potentially identified in growth-dependent, blood group A-like GalNAc glycan-bearing, ovarian glycolipids complementary with the syngeneic anti-A reactive IgM, which does not appear in early ovariectomized animals. This non-somatically encoded, polyreactive, ancestral IgM molecule has not undergone clonal selection and does primarily not differentiate between self and non-self and might, due to amino acid hydroxyl groups, highly suggest substrate competition with subsequent O-glycosylations in ongoing ESC-GC transformations and affecting GC maturation. However, the membrane-bound somatic N/O-glycotransferases, which initiate, after formation of the zygote, the complex construction of the human ABO phenotypes in the trans cisternae of the Golgi apparatus, are associated and/or completed with soluble enzyme versions exerting identical specificities in plasma and likely competing vice versa by glycosylation of neonatal IgM amino acids, where they suggest to accomplish the clearance of anti

  14. Overcoming interspecific incompatibility in the cross Brassica campestris ssp. japonica x Brassica oleracea var. botrytis using irradiated mentor pollen page

    The cross B. campestris ssp. japonica x B. oleracea var. botrytis fails due to incompatibility barrier at the stigma. To realize this cross, irradiated compatible pollen (mentor pollen) was used before the incompatible pollination. The presence of mentor pollen stimulated the incompatible pollen to germinate and effect fertilization and seed set. One hybrid was thus obtained. Most of the seeds were inviable. Of the 5 plants raised one was a hybrid and 4 resembled the female parent. 1 tab., 7 refs

  15. Genetic evidence that two independent S-loci control RNase-based self-incompatibility in diploid strawberry

    Bošković, Radovan I.; Sargent, Daniel J.; Tobutt, Kenneth R.

    2009-01-01

    The self-incompatibility mechanism that reduces inbreeding in many plants of the Rosaceae is attributed to a multi-allelic S locus which, in the Prunoideae and Maloideae subfamilies, comprises two complementary genes, a stylar-expressed S-RNase and a pollen-expressed SFB. To elucidate incompatibility in the subfamily Rosoideae, stylar-specific RNases and self-(in)compatibility status were analysed in various diploid strawberries, especially Fragaria nubicola and F. viridis, both self-incompat...

  16. Evaluation of physicochemical incompatibilities during parenteral drug administration in a paediatric intensive care unit.

    Gikic, M; Di Paolo, E R; Pannatier, A; Cotting, J

    2000-06-01

    Patients in paediatric intensive care units (PICU) often receive numerous medications by the parenteral route. Frequently two or more drugs are delivered simultaneously through the same line and the risk of physicochemical incompatibilities is thus important. The objectives of this study were 1) to identify prospectively the combinations of injectable drugs administered in the PICU of our university hospital and 2) to analyze them according to information found in the literature. The data were collected by a pharmacist over a 30-day period and classified in three categories: compatible, incompatible and undocumented. Nineteen patients were included in the study with a median age of 3.2 years. The mean number (+/- SD) of injectable drugs per patient and per day was 6.5 (+/- 2.8), for a total of 26 drugs and 7 solutes. 64 combinations of drugs were observed with 2 (31.3%), 3 (45.3%), 4 (10.9%) or 5 (12.5%) drugs. 81 drug-drug and 94 drug-solute combinations were recorded. Among these, 151 (86.3%) were compatible, 6 (3.4%) incompatible and 18 (10.3%) undocumented. The incompatibilities included furosemide (Lasix), a drug in alkaline solution and Vamina-Glucose, a total parenteral nutrition solution. No clinical consequences resulting from drug incompatibilities were shown in this study. We suggest that in vitro compatibility tests on standard drug combinations, as well as a training program for nurses on drug incompatibility problems would sensitively increase the security of parenteral drug administration. PMID:11028261

  17. Natural variation of heterokaryon incompatibility gene het-c in Podospora anserina reveals diversifying selection.

    Bastiaans, Eric; Debets, Alfons J M; Aanen, Duur K; van Diepeningen, Anne D; Saupe, Sven J; Paoletti, Mathieu

    2014-04-01

    In filamentous fungi, allorecognition takes the form of heterokaryon incompatibility, a cell death reaction triggered when genetically distinct hyphae fuse. Heterokaryon incompatibility is controlled by specific loci termed het-loci. In this article, we analyzed the natural variation in one such fungal allorecognition determinant, the het-c heterokaryon incompatibility locus of the filamentous ascomycete Podospora anserina. The het-c locus determines an allogenic incompatibility reaction together with two unlinked loci termed het-d and het-e. Each het-c allele is incompatible with a specific subset of the het-d and het-e alleles. We analyzed variability at the het-c locus in a population of 110 individuals, and in additional isolates from various localities. We identified a total of 11 het-c alleles, which define 7 distinct incompatibility specificity classes in combination with the known het-d and het-e alleles. We found that the het-c allorecognition gene of P. anserina is under diversifying selection. We find a highly unequal allele distribution of het-c in the population, which contrasts with the more balanced distribution of functional groups of het-c based on their allorecognition function. One explanation for the observed het-c diversity in the population is its function in allorecognition. However, alleles that are most efficient in allorecognition are rare. An alternative and not exclusive explanation for the observed diversity is that het-c is involved in pathogen recognition. In Arabidopsis thaliana, a homolog of het-c is a pathogen effector target, supporting this hypothesis. We hypothesize that the het-c diversity in P. anserina results from both its functions in pathogen-defense, and allorecognition. PMID:24448643

  18. Presence of nucleotide substitutions in the ABO promoter in individuals with phenotypes A3 and B3.

    Isa, K; Yamamuro, Y; Ogasawara, K; Yabe, R; Ogiyama, Y; Ito, S; Takahashi, Y; Kominato, Y; Sano, R; Uchikawa, M

    2016-04-01

    Recently, the involvement of mutation and deletion of transcription regulatory elements in the Bm , Am , A3 and B3 phenotypes has been reported. In the present study, we carried out genetic analysis of individuals with A3 and B3 using peptide nucleic acid-clamping PCR to exclude amplification of O alleles. Two single-point mutations, -76G>C and -68G>T, were found in the ABO promoter on the A-allele in three A3 individuals and on the B allele in a B3 individual, respectively. Transient transfection of luciferase reporter plasmids carrying the same mutations into K562 cells revealed decreased luciferase activity in comparison with that carrying the wild-type promoter. These observations suggest that the mutations downregulate the promoter activity, leading to reduction in A- or B-antigen expression on red blood cells in individuals with the A3 and B3 phenotypes. PMID:26529276

  19. Distinguishing early-acting inbreeding depression from late-acting ovar-ian self-incompatibility

    Yiqi Hao; Xinfeng Zhao

    2011-01-01

    Reduced seed yields after self-pollination are generally thought to be induced by early-acting in-breeding depression and self-incompatibility. Early-acting inbreeding depression occurs strictly post-zygoti- cally, and leads to the abortion of progeny that are homozygous for deleterious recessive alleles at an early stage of seed maturation. Late-acting ovarian self-incompatibility, on the other hand, may be either pre- or post-zygotic, and usually only one locus is responsible for the reject...

  20. Passenger lymphocyte syndrome in ABO and Rhesus D minor mismatched liver and kidney transplantation: A prospective analysis.

    ElAnsary, Mervat; Hanna, Mariam Onsy F; Saadi, Gamal; ElShazly, Mostafa; Fadel, Fatina I; Ahmed, Hanan AbdElAziz; Aziz, Amr Mostafa; ElSharnouby, Amal; Kandeel, Mona MohiElDin T

    2015-06-01

    The increasing demand for solid organs has necessitated the use of ABO and Rhesus (Rh) D minor mismatched transplants. The passenger lymphocyte syndrome (PLS) occurs when donor lymphocytes produce antibodies that react with host red blood cell (RBC) antigens and result in hemolysis. Our aim was to evaluate prospectively the role of PLS in post transplant anemia and hemolysis in ABO and RhD minor mismatched recipients of liver and kidney grafts and to study the association of PLS with donor lymphocyte microchimerism. We examined 11 liver and 10 kidney recipients at Day +15 for anemia, markers of hemolysis, direct antiglobulin test and eluates, and serum RBC antibodies. Microchimerism was determined in peripheral blood lymphocytes by genotyping of simple sequence length polymorphisms encoding short tandem repeats. Immune hemolytic anemia and anti-recipient RBC antibodies were observed in 2 out of 11 liver (18.2%) and 2 out of 10 kidney (20%) transplants. RBC antibody specificity reflected the donor to recipient transplant, with anti-blood group B antibodies identified in 2 cases of O to B and 1 case of A to AB transplants while anti-D antibodies were detected in 1 case of RhD-negative to RhD-positive transplant. Donor microchimerism was found in only 1 patient. In conclusion, passenger lymphocyte mediated hemolysis is frequent in minor mismatched liver and kidney transplantation. Recognizing PLS as a potential cause of post transplant anemia may allow for early diagnosis and management to decrease the morbidity and mortality in some patients. PMID:25842056

  1. Simultaneous forward and reverse ABO blood group typing using a paper-based device and barcode-like interpretation.

    Songjaroen, Temsiri; Laiwattanapaisal, Wanida

    2016-05-19

    A new platform of a paper-based analytical device (PAD) for simultaneous forward and reverse ABO blood group typing has been reported. This platform can overcome the discrepancy results as influenced by the individual haematocrit. The test and the control of non-haemagglutination on each channel were performed in parallel. The PAD was fabricated by printing six parallel channels with wax onto Whatman No. 4 filter paper. An LF1 blood separation membrane was used for the separation of plasma from whole blood for reverse grouping. The blood group was identified by haemagglutination of the corresponding antigen-antibody. For forward grouping, Anti-A, -B and -A,B were treated on the test line of PAD, and inactivated Anti-A, -B and -A,B were immobilized on the control line. For reverse grouping, 30% standard A-cells, B- and O- were added to the test channel after plasma separation, and O-cells were used as a control. Then, 0.9% normal saline (NSS) containing 1% Tween-20 was bi-functionally used for dilution of the blood sample and elution of the non-agglutinated RBCs within the channels. The distance of agglutinated RBCs in each test line was compared with the distance of non-agglutinated RBCs in the parallel control line. The forward and reverse patterns of blood groups A, B, AB and O were a barcode-like chart in which the results can be visually analysed. The PAD has excellent reproducibility when 10 replications of the A, B, AB or O blood groups were performed. The results of both forward and reverse grouping were highly correlated with conventional methods compared with the slide method and tube method, respectively (n = 76). Thus, this ABO typing PAD holds great potential for future applications in blood typing point-of-care testing. PMID:27126791

  2. ABO血型鉴定不一致原因分析及解决方法%ABO Blood Group Identification ;not Consistent Cause Analysis

    万会林

    2013-01-01

    目的:分析ABO血型鉴定不一致原因分析及解决方法方法:应用ABO血型鉴定原理即根据红细胞表面有无A抗原和(或)B抗原,将血型分为A型、B型、AB型及O型4种,可利用红细胞的凝集实验,通过正反定型准确鉴定ABO血型。结果:笔者就几年血型不一致的原因进行分析,并加以分别探讨。结论:正确鉴定ABO血型是输血的关键之一,误输异型血可使受血者发生严重的输血反应,危及病人生命安全。对于ABO血型鉴定不一致的,无法判断的特殊血型标本,可以针对其原因,采取多种方法正确鉴定ABO血型。%objective:To analyse ABO blood group identifica-tion not consistent cause analysis and solution method:the applica-tion of ABO blood group identification principle according to red blood cells that have A surface antigen and (or) B antigens, will be di-vided into A blood type, type B, type AB and type O four, can use of red blood cells agglutinate experiment, through the positive and neg-ative stereotypes accurate identification ABO blood group. Results:the author is not consistent with a few years blood type analysis of the causes, and to study. Conclusion:correctly identify the ABO blood group is one of the keys to a blood transfusion, abnormal blood can make false input by the blood transfusion reactions happen serious, endanger patient safety. For the ABO blood group appraisal does not agree, can't judge blood type specimens of the special, can't judge of the special specimens, can according to the cause and take DuoZhong method correctly identify the ABO blood group.

  3. Identification of differentially expressed genes involved in self-incompatibility in Theobroma cacao L.

    Increasing yield, quality and disease resistance are important objectives for cacao breeding programs. However, self-incompatibility (SI) often restricts progress, as crosses between certain cacao germplasm accessions and breeding lines are only partially successful. Various events are involved in t...

  4. Graft Incompatibility Influence on Assimilating Pigments and Soluble Sugars Amount of some Pear (Pyrus sativa Cultivars

    Gheorghii CIOBOTARI

    2010-06-01

    Full Text Available Graft incompatibility in fruit trees is one of the greatest obstacles in rootstocks and cultivars breeding. The mechanism in which incompatibility is expressed is not yet fully understood and several hypotheses have been advanced in an attempt to explain it. In many cases (pear on quince grafts, apricot on Prunus grafts, incompatibility is manifested by the breaking of the trees at the point of the union particularly when they have been growing for some years. Many reports focus on this problem in order to understand the mechanisms of graft development. These reports refer to both cytological and biochemical responses occurring at an early phase in response to grafting, as well as to the consequences of these events on the future graft response. In this experiment, we tried to highlight how affinity between scion and rootstock can influence the photosynthetic apparatus and carbohydrates synthesis. The results showed that grafting affinity has an influence on total assimilating pigments content. Thus, on the pear cultivars grafted on an incompatible rootstock (cultivars/Cydonia oblonga the total pigments content ratio (reported to the ungrafted rootstock ranged between 0.58 and 0.69. However, the combinations had a ratio ranging between 0.79 and 0.98. Nevertheless, the assimilating pigments ratio reduction had no influence on photosynthetic rate. The soluble sugars amount was close in both variants (cultivars/Cydonia oblonga and cultivars/Pyrus sativa.

  5. Incompatibility of water soluble contrast media and intravascular pharmacologic agents: experimental study

    With the development of low osmolar and nonionic contrast media in clinical practice, radiologists have enjoyed increased clinical application along with other advantages such as improvement of patient comfort and safety. Recently, radiologists have introduced many intravascular pharmacologic agents to improve diagnostic quality and patient safety. Shortly after the introduction of these agents, however, it was observed that a precipitate results when a certain pharmacologic agent is mixed with a low osmolar contrast media. These observations have prompted testing of several other drugs used for incompatibility with contrast media. To verify these reports and to investigate other medications not previously tested, the authors analyzed mixtures of contrast agents and medications in vitro and observed them for visible precipitates after operating the high-speed centrifuge. The results were as fallows: The contrast media that produced incompatibilities with some pharmacologic agents were ioxaglate, diatrizote, and iothalamate in the order of frequency. The contrast media that produced no precipitate were iopromide and ioxithalamate. The pharmacologic agents that produced precipitate with some contrast media were Papaverine, Benadryl, Protamine, Cimetidine, Regitin, and Gentamicin. Therefore, we recommend that caution should be taken to recognize incompatibilities and avoid them when intravascular pharmacologic agents of any kind may be incompatible

  6. Inhibiting Self-Pollen: Self-Incompatibility in Papaver Involves Integration of Several Signaling Events

    2007-01-01

    Cellular responses rely on signal perception and integration. A nice example of this is self incompatibility (SI), which is an important mechanism to prevent inbreeding. It prevents self-fertilization by using a highly discriminatory cellular recognition and rejection mechanism. Most S1 systems are genetically specified by the S-locus, which has a pollen and a pistil S-component. A receptor-ligand interaction is used by Papaver rhoeas to control SI. S proteins encoded by the pistil part of the S-locus interact with incompatible pollen to achieve rapid inhibition of tip growth. The incompatible SI interaction triggers a Ca2+ -dependent signaling cascade. A number of SI-specific events are triggered in incompatible pollen, including rapid depolymerization of the actin cytoskeleton; phosphorylation of soluble inorganic pyrophosphatases (SPPases), Prp26.1; activation of a mitogen activated protein kinase, p56; programmed cell death (PCD) involving a caspase-3-like activlty. These events contribute to prevent self-fertilization. We are attempting to establish the functional signiflcance of these events, and their possible involvement in integrating a coordinated signaling response. Here we describe the identification of these components shown to be involved in SI, together with recent progress in identifying links between some of them. These data constitute the first steps in elucidating how SI signaling is integrated.

  7. 40 CFR Appendix V to Part 265 - Examples of Potentially Incompatible Waste

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Examples of Potentially Incompatible Waste V Appendix V to Part 265 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED..., STORAGE, AND DISPOSAL FACILITIES Pt. 265, App. V Appendix V to Part 265—Examples of...

  8. A co-modelling method for solving incompatibilities during co-design of mechatronic devices

    Ni, Yunyun; Broenink, Jan F.

    2014-01-01

    The design process of mechatronic devices, which involves experts from different disciplines working together, has limited time and resource constraints. These experts normally have their own domain-specific designing methods and tools, which can lead to incompatibilities when one needs to work toge

  9. Male and female roles in crosses of Aspergillus nidulans as revealed by vegetatively incompatible parents

    Bruggeman, I.M.; Debets, A.J.M.; Swart, K.; Hoekstra, R.F.

    2003-01-01

    To resolve the role of male and female nuclei and mitochondria in cleistothecium formation in the model organism Aspergillus nidulans, we analysed the genetic constituents of cleistothecia, from crosses between vegetatively compatible and incompatible parents. We used markers that enabled us to dete

  10. How to produce quantum entanglement for ascertaining incompatible properties in double-slit experiments

    Nisticò, Giuseppe

    2010-01-01

    Double-slit experiment very well lends itself in describing the problem of measuring simultaneously incompatible properties. In such a context, we theoretically design an ideal experiment for spin-7/2 particles, able to produce the entanglement which makes possible the detection.

  11. Simultaneous non-disturbing detection of incompatible properties in double-slit experiment

    Nistico', Giuseppe; Sestito, Angela

    2003-01-01

    We exploit the notion of which-slit detector introduced by Englert, Scully and Walther (ESW), to show that two incompatible properties can be detected together for each particle hitting the screen, without disturbing the center-of-mass motion of the particle.

  12. On the Incompatibility of Standard Quantum Mechanics and the de Broglie-Bohm Theory

    Ghose, Partha

    2001-01-01

    It is shown that the de Broglie-Bohm quantum theory of multi-particle systems is incompatible with the standard quantum theory of such systems unless the former is ergodic. A realistic experiment is suggested to distinguish between the two theories.

  13. Ending tolerance as a solution to incompatibility: The Danish ‘crisis of multiculturalism’

    Hervik, Peter

    2012-01-01

    group discussion in Denmark on the integration of Muslims into Danish society. This idea of incompatibility between native Danes and Muslim ‘newcomers’ has become a salient feature of what is termed ‘value-based journalism’ and ‘value-based politics’ in the last decade. This article traces the origin...

  14. Incompatibility between Zircaloy-2 and Inconel X-750 during Temperature Transients

    Warren, M. R.; Rørbo, Kaj; Adolph, Eivind

    1975-01-01

    The incompatibility of Zircaloy-2 and Inconel X-750 has been investigated between 1000°C and 1200°C (1200°C being the currently allowable maximum temperature in the acceptance criteria for ECCS for water reactors). It has been found for the temperatures of 1000°C and 1200°C that oxide thicknesses...

  15. The effect of Wolbachia-induced cytoplasmic incompatibility on host population size in natural and manipulated systems.

    Dobson, Stephen L.; Fox, Charles W; Jiggins, Francis M

    2002-01-01

    Obligate, intracellular bacteria of the genus Wolbachia often behave as reproductive parasites by manipulating host reproduction to enhance their vertical transmission. One of these reproductive manipulations, cytoplasmic incompatibility, causes a reduction in egg-hatch rate in crosses between individuals with differing infections. Applied strategies based upon cytoplasmic incompatibility have been proposed for both the suppression and replacement of host populations. As Wolbachia infections ...

  16. Distribution of von Willebrand factor levels in young women with and without bleeding symptoms: influence of ABO blood group and promoter haplotypes

    Lethagen, S.; Hillarp, A.; Ekholm, C.;

    2008-01-01

    . It was the objective of the present study to evaluate the distribution of VWF levels in young females with or without bleeding symptoms in this population, and the influence of ABO blood group and promoter haplotypes on VWF levels and to identify a possible increased prevalence of VWD in females with bleeding symptoms.......4%) (p = 0.017). Blood group O was found in 14/18 girls with low VWF:RCo. There was a highly significant correlation between VWF:RCo and blood group O and non-O genotypes. Two common VWF promoter haplotypes did not contribute to the VWF:RCo variation. VWF levels did not correlate with time during...... menstrual cycle, or the use of oral contraceptives. No case fulfilled the diagnostic criteria for VWD. In conclusion, low VWF:RCo was significantly more frequent in females with bleeding symptoms. However, we found no case fulfilling strict diagnostic criteria for VWD. The ABO blood group was a strong...

  17. Naturally occurring incompatibilities between different Culex pipiens pallens populations as the basis of potential mosquito control measures.

    Lin Chen

    Full Text Available BACKGROUND: Vector-borne diseases remain a threat to public health, especially in tropical countries. The incompatible insect technique has been explored as a potential control strategy for several important insect vectors. However, this strategy has not been tested in Culex pipiens pallens, the most prevalent mosquito species in China. Previous works used introgression to generate new strains that matched the genetic backgrounds of target populations while harboring a new Wolbachia endosymbiont, resulting in mating competitiveness and cytoplasmic incompatibility. The generation of these incompatible insects is often time-consuming, and the long-term stability of the newly created insect-Wolbachia symbiosis is uncertain. Considering the wide distribution of Cx. pipiens pallens and hence possible isolation of different populations, we sought to test for incompatibilities between natural populations and the possibility of exploiting these incompatibilities as a control strategy. METHODOLOGY/PRINCIPAL FINDINGS: Three field populations were collected from three geographic locations in eastern China. Reciprocal cross results showed that bi-directional patterns of incompatibility existed between some populations. Mating competition experiments indicated that incompatible males could compete with cognate males in mating with females, leading to reduced overall fecundity. F1 offspring from incompatible crosses maintained their maternal crossing types. All three populations tested positive for Wolbachia. Removal of Wolbachia by tetracycline rendered matings between these populations fully compatible. CONCLUSIONS/SIGNIFICANCE: Our findings indicate that naturally occurring patterns of cytoplasmic incompatibility between Cx. pipiens pallens populations can be the basis of a control strategy for this important vector species. The observed incompatibilities are caused by Wolbachia. More tests including field trials are warranted to evaluate the feasibility

  18. ABO histo-blood groups and Rh systems in relation to malignant tumors of the digestive tract in Bosnia and Herzegovina

    Jovanović-Ćupić Snežana; Stamenković Gorana; Blagojević Jelena; Vanis N.; Stanojević B.; Berberović Lj.

    2008-01-01

    The distribution of ABO blood groups and the Rhesus factor was analyzed in 279 patients who suffered from malignant tumors of the digestive system. Patients were registered retrospectively in the Gastroenterohepatology Clinic, Clinical Center, University of Sarajevo over a discontinuous period of 88 months. From the results obtained, it was concluded that: (a) men became ill from gastric cancer significantly more frequently than women; (b) the frequency of liver carcinoma was three times high...

  19. Distribution of ABO and rhesus (D blood group antigens among blood donors at a tertiary care teaching hospital blood bank in south India

    Suresh B

    2015-04-01

    Full Text Available Background: The ABO and Rhesus (Rh blood group systems are important for transfusion of blood and its components, organ transplantation, genetic studies and in medico-legal issues. Despite the long list of several other blood groups discovered so far, the knowledge and distribution of ABO and Rh-D blood group are essential for effective management of blood bank inventory. Methods: We retrospectively studied the distribution of ABO and Rh blood group antigens in donors presenting to our tertiary care teaching hospital blood bank in south India during the period January 2007 to August 2014. Blood group was determined by commercially available standard monoclonal antisera by test tube agglutination technique. Results: A total of 49,110 donor samples were tested during the study period for ABO grouping and Rh-D typing. Out of these 96.9% were males. The frequency of O, B, A, AB and Bombay blood groups were 41.7%, 32.2% 20%, 6.1% and 0.03% respectively. Rh (D positive and negative blood groups were seen in 92.8% and 7.2% respectively. The allele frequencies of the I A , IB and IO alleles were 0.1398, 0.2148 and 0.6454 respectively. In case of Rh-D group, the calculated gene frequencies for ID and Id were 0.7321 and 0.2679 respectively. Conclusion: Knowledge of blood group systems as documented in the present study helps in efficient management of blood bank and transfusion services in emergencies.

  20. Trans-National Genetic Distance and Genetic Identity of Barak Valley Hindus en Route the Journey of Mankind from Africa for ABO Gene

    Supriyo CHAKRABORTY

    2011-08-01

    Full Text Available The present study aimed at estimating the genetic distance and genetic identity between Barak Valley Hindus and other twenty four nations for ABO blood group gene along the route of historic journey of mankind from Africa as proposed by Stephen Oppenheimer to gain insights on the evolutionary relationship and genetic closeness of the Hindus with other nations. Barak Valley Zone, located in southern part of Assam state in North East India, has inhabited the major endogamous group, the Hindus, for several centuries. Over the last few decades, they have maintained distinct culture and life style. This study used ABO gene frequency data of these populations to estimate Nei�s standard genetic distance and genetic identity of population genetics between Barak Valley Hindus and other nations. The historic journey of mankind commenced from Africa about 200,000 years ago (www.bradshawfoundation.com. Genetic distance estimate ranged from 0.07 to 5.18%. Barak Valley Hindus (BVH showed relatively low genetic distance for ABO gene with the populations of Saudi Arabia (0.07%, India (0.13%, Borneo (0.40%, Russia (0.59%, Central Asia (0.60%, Siberia (0.60%, South China (0.71% and Sri Lanka (0.93% suggesting high genetic identity and possible evolutionary relationship of BVH during migration with these nations. But the BVH showed highest genetic distance with Australia (5.18% followed by Norway (4.13%, Sudan (3.89% and Sweden (3.60% indicating low genetic identity of BVH with these nations. Migration was not the key determining factor in changing the ABO gene frequency in human populations.

  1. Distribution of ABO and Rh-D blood groups in the Benin area of Niger-Delta: Implication for regional blood transfusion

    Enosolease Mathew

    2008-01-01

    Full Text Available ABO and Rhesus (Rh blood group antigens are hereditary characters and are useful in population genetic studies, in resolving medico-legal issues and more importantly in compatibility test in blood transfusion practice. Data on frequency distribution of ABO and Rh-D in Niger-Delta region of Nigeria are not available; hence we made an attempt to retrospectively analyze the records on the blood donors, transfusion recipients and patients attending antenatal care or some other medical interventions. Over a twenty-year period between 1986 and 2005, a total of 160,431 blood samples were grouped for ABO and Rh-D at the blood bank of the University of Benin Teaching Hospital, Benin City, Nigeria. Blood group distribution among these samples showed phenotypes A, B, AB and O as 23.72%, 20.09%, 2.97% and 53.22%, respectively. The Rh-D negative phenotype was found among 6.01% of the samples tested.

  2. Servicemembers' Group Life Insurance--Veterans' Group Life Insurance regulation update--ABO, VGLI application, SGLI 2-year disability extension. Final rule.

    2014-07-31

    This document amends the Department of Veterans Affairs (VA) insurance regulations concerning Servicemembers' Group Life Insurance (SGLI) to reflect the statutory provisions of the Veterans' Benefits Act of 2010, which became law on October 13, 2010, and resulted in the need for amendments to change the SGLI Disability Extension period from 1 year to 2 years in duration; provide SGLI Traumatic Injury Protection (TSGLI) retroactive coverage effective from October 7, 2001, for all qualifying injuries regardless of the geographic location and military operation in which the injuries were incurred; and remove the SGLI and Veterans' Group Life Insurance (VGLI) Accelerated Benefits Option (ABO) discount rate. This rule also clarifies that ``initial premium'' refers to ``initial Veterans' Group Life Insurance premium,'' updates the current address of the Office of Servicemembers' Group Life Insurance (OSGLI), managed by Prudential Insurance Company of America, to reflect where the ABO application is mailed for processing, and corrects the OSGLI phone number. Finally, this rule removes the ABO application form from the regulation, and it corrects and clarifies language concerning the VGLI application period that was inadvertently incorrectly modified in a prior amendment of the regulations. PMID:25122940

  3. Neuroblastomicose: registro de três casos

    Ehrenfried O. Wittig

    1968-03-01

    Full Text Available São relatados três casos de neuroblastomicose de sintomatologia variada (urna forma tumoral cerebelar, urna forma abscedante troncular e cerebral e urna forma granulomatosa difusa. No caso da forma tumoral cerebelar havia associação com cisticercose cerebral. Alterações medulares não foram evidenciadas no único caso em que foi possível examinar êste setor do sistema nervoso central (caso 2.

  4. y casos de estudios

    Esteban Valenzuela Van Treek

    2006-01-01

    latinoamericanas, adentrándose en las problemáticas de la más relevantes, así como reflexionar sobre el presente de ellas, tomando para esto algunos casos de estudio.. “En el caso de las áreas metropolitanas, la concentración y el crecimiento de las grandes ciudades con fenómenos de nuevas barriadas, de municipios limítrofes y de ciudades dormitorios, dentro o fuera del término de la gran ciudad, demandan soluciones administrativas de conjunto que superen los esquemas municipales tradicionales” (Barrero, 1993. Esto lleva a definir el problema de estudio de como la aglomeración de población en áreas superiores a las ciudades tradicionales en América Latina, constituyen formas diferentes a los planos nacionales y municipales, por lo que urge crear condiciones para el bienestar de sus ciudadanos. Es necesario para esto, el comprender el sistema político de manera dinámica y variable, el cual esta íntimamente ligado a la constitución a la deriva estructural de las sociedades, pero sobretodo al gobierno de la ciudad, elemento fundante de la civilización occidental en la cual estamos situados.

  5. MEXCODE* CASO DE ESTUDIO

    Gabriel Arturo García Gómez

    2000-01-01

    Full Text Available El caso plantea la reactivación de un proyecto que se presentó en el año 1995 como alternativa para el desarrollo del centro del Valle y mejora de la eficiencia en el manejo del comercio exterior de la Cuenca Pacífica. El proyecto se revivió a raíz de la necesidad de la compañía patrocinadora de mejorar sus flujos de caja proyectados y la reciente autorización para su funcionamiento. El proyecto es el popularmente denominado “Puerto Seco” de la ciudad de Guadalajara de Buga, internamente nombrado Mexcode. Los estudios iniciales estaban encaminados a que el proyecto fuera manejado por el municipio de Buga, sin embargo, posteriormente se vio la necesidad de buscar que pasara a manos de la empresa privada. El municipio, después de analizar las propuestas de varios inversionistas de la región en las cuales se evaluaba la ubicación dentro de la zona de los predios de sus empresas, su área, proximidad a la línea férrea y a la doble calzada Buga-Tuluá (sitio de paso del 80% de la carga de importación y exportación por Buenaventura y evaluar aspectos relativos a su trayectoria empresarial y compromiso con el desarrollo de la región, decidió otorgarle el proyecto al Grupo Empresarial Agri-Supplies S.A.

  6. Localized graft incompatibility in pear/quince (Pyrus communis/Cydonia oblonga) combinations: multivariate analysis of histological data from 5-month-old grafts.

    Ermel, F. F.; Kervella, J.; Catesson, A. M.; Poëssel, J. L.

    1999-08-01

    To characterize the structural events associated with incompatibility of graft development, we conducted a histological study of compatible and incompatible pear/pear and pear/quince grafts that had been grown for five months in a greenhouse. Multivariate analysis of histological data describing the structure of the graft union allowed us to discriminate between compatible and incompatible combinations before either macroscopic examination or qualitative microscopic examination differences between graft combinations became evident. The histological variables responsible for the discrimination between incompatible and compatible unions were related to three typical symptoms of graft incompatibility: bark discontinuity, which was the main feature; cambial dysfunction; and accumulation of starch in the scion. Little cell necrosis was observed at the interface of incompatible grafts at the 5-month stage of graft development. Multivariate analysis of histological data provides a new tool for studying early structural events resulting from the graft incompatibility response and for diagnosing early graft incompatibility. PMID:12651320

  7. Ictericia e incompatibilidad por grupo Kell: caso clínico.

    Miguel Zúñiga

    2010-08-01

    Full Text Available Dentro de las patologías más frecuentes que afectan al recién nacido se encuentra la ictericia. Sus etiologías son diversas y conforman una larga lista que varía desde causas infecciosas, metabólicas, hematológicas, inmunológicas, entre otras. Sin embargo, cuando se presenta durante las primeras horas, las causas más frecuentes corresponden a incompatibilidades de grupo sanguíneo y de ellas, la incompatibilidad Rh y ABO suelen ser más frecuentes. La incompatibilidad por grupo Kell, en cambio, es un trastorno mucho menos frecuente. Se presenta en un recién nacido con ictericia secundaria a una anemia hemolítica y que posee anticuerpos relacionados con el grupo sanguíneo Kell. Su evolución puede ser compleja y severa, requiriendo en algunos casos manejo en cuidados intensivos y apoyo importante de hemoderivados. Todo lo anterior hace importante conocer esta patología, de manera de poder identificarla y actuar a tiempo en su tratamiento.

  8. Cytoplasmic Incompatibility as a Means of Controlling Culex pipiens quinquefasciatus Mosquito in the Islands of the South-Western Indian Ocean

    Atyame, Célestine M.; Nicole Pasteur; Emilie Dumas; Pablo Tortosa; Michaël Luciano Tantely; Nicolas Pocquet; Séverine Licciardi; Ambicadutt Bheecarry; Betty Zumbo; Mylène Weill; Olivier Duron

    2011-01-01

    The use of the bacterium Wolbachia is an attractive alternative method to control vector populations. In mosquitoes, as in members of the Culex pipiens complex, Wolbachia induces a form of embryonic lethality called cytoplasmic incompatibility, a sperm-egg incompatibility occurring when infected males mate either with uninfected females or with females infected with incompatible Wolbachia strain(s). Here we explore the feasibility of the Incompatible Insect Technique (IIT), a species-specific...

  9. SELF INCOMPATIBILITY MECHANISMS IN THE CROCUS SATIVUS AGGREGATE (IRIDACEAE: A PRELIMINARY INVESTIGATION

    R. ZANIER

    2000-01-01

    Full Text Available Two molecular mechanisms responsible for SI (Self-Incompatibility in dicotyledons were tested in the C. sativus L. aggregate. RNase and peroxidase activity assays were carried out on crude extract from un-, self- and cross-pollinated styles of C. sativus (male-sterile, C. thomasii Ten. (outfertile and C. cartwrightianus Herb (out-fertile. Results on RNase activity indicate that in the Crocus species studied the rejection mechanism of SI is not based on stylar RNase. Data on peroxidase activity indicate a relationship between pollen tube presence in the style and stylar peroxidase activity. Stylar peroxidase activity increase is related to pollen tube presence but does not stop tube growth. Compatible and incompatible pollen tubes grow along the style and their discrimination occurs in another region of the gynoecium.

  10. Partition-associated incompatibility caused by random assortment of pure plasmid clusters

    Ebersbach, Gitte; Sherratt, David J; Gerdes, Kenn;

    2005-01-01

    Summary Bacterial plasmids and chromosomes encode centromere-like partition loci that actively segregate DNA before cell division. The molecular mechanism behind DNA segregation in bacteria is largely unknown. Here we analyse the mechanism of partition-associated incompatibility for plasmid pB171......, a phenotype associated with all known plasmid-encoded centromere loci. An R1 plasmid carrying par2 from plasmid pB171 was destabilized by the presence of an F plasmid carrying parC1, parC2 or the entire par2 locus of pB171. Strikingly, cytological double-labelling experiments revealed no evidence of long......-lived pairing of plasmids. Instead, pure R1 and F foci were positioned along the length of the cell, and in a random order. Thus, our results raise the possibility that partition-mediated plasmid incompatibility is not caused by pairing of heterologous plasmids but instead by random positioning of pure plasmid...

  11. Physiological and Cytological Similarities between Disease Resistance and Cellular Incompatibility Responses.

    Teasdale, J; Daniels, D; Davis, W C; Eddy, R; Hadwiger, L A

    1974-11-01

    Excised pea pods responded similarly to both the invasion of plant pathogenic fungi and the presence of bean tissue, bean pollen, and mouse tumor cells by synthesizing pisatin and by developing a characteristic yellow-green fluorescence. Both responses were dependent on RNA and protein synthesis. Conversely, the foreign pollen and incompatible fungi were sensitive to the pea pod tissue and were subject to abnormal development.The induction of pisatin and the yellow-green fluorescence development were mediated by multiple compounds of varying sizes released by fungi or mouse tumor cells. The incompatibility between a bean pathogen, Fusarium solani f. sp. phaseoli, and pea pod tissue was hypothesized to occur as a result of the cross contamination of such inducing compounds. PMID:16658953

  12. Study of incompatibility of ammonium nitrate and its mechanism of decomposition by theoretical approach

    Cagnina, Stefania; ROTUREAU, Patricia; Adamo, Carlo

    2013-01-01

    The dramatic accident involving ammonium nitrate (AN) that took place at Toulouse in September 2001 has once again focused attention to the complex hazards pertaining to this chemical. Despite the significant efforts made to increase AN safety over the whole supply chain, we are still facing insufficient knowledge of the actual mechanisms of ammonium nitrate decomposition that may take place in abnormal situations. The paper proposes a theoretical study of chemical incompatibilities of ammoni...

  13. Interactions and incompatibilities of pharmaceutical excipients with active pharmaceutical ingredients: a comprehensive review.

    Bharate, Sonali S.; Bharate, Sandip B.; Bajaj, Amrita N.

    2010-01-01

    Studies of active drug/excipient compatibility represent an important phase in the preformulation stage of the development of all dosage forms. The potential physical and chemical interactions between drugs and excipients can affect the chemical nature, the stability and bioavailability of drugs and, consequently, their therapeutic efficacy and safety. The present review covers the literaturereports of interaction and incompatibilities of commonly used pharmaceutical excipients with different...

  14. Morphological Characteristics and Somatic Incompatibility of Ganoderma from Infected Oil Palm from Three Inland Estates

    Latiffah, Z.

    2005-01-01

    Full Text Available The morphological characteristics of Ganoderma basidiomata from infected oil palms from three inland estates showed some variations, but all fall within the description of G. boninense, based on Steyaert s classification system (1967, 1975. Pairings of G. boninense isolates from the same estate showed that there was somatic incompatibility among the isolates which indicated that the isolates were distinct individuals and not clones of single genotypes.

  15. Cryptic self-incompatibility and distyly in Hedyotis acutangula Champ. (Rubiaceae).

    Wu, X; Li, A; Zhang, D

    2010-05-01

    Distyly, floral polymorphism frequently associated with reciprocal herkogamy, self- and intramorph incompatibility and secondary dimorphism, constitutes an important sexual system in the Rubiaceae. Here we report an unusual kind of distyly associated with self- and/or intramorph compatibility in a perennial herb, Hedyotis acutangula. Floral morphology, ancillary dimorphisms and compatibility of the two morphs were studied. H. acutangula did not exhibit precise reciprocal herkogamy, but this did not affect the equality of floral morphs in the population, as usually found in distylous plants. Both pin and thrum pollen retained relatively high viability for 8 h. The pollen to ovule ratio was 72.5 in pin flowers and 54.4 in thrum flowers. Pistils of pin flowers remained receptive for longer than those of thrum flowers. No apparent difference in the germination rate of pin and thrum pollen grains was observed when cultured in vitro, although growth of thrum pollen tubes was much faster than that of pin pollen tubes. Artificial pollination revealed that pollen tube growth in legitimate intermorph crosses was faster than in either intramorph crosses or self-pollination, suggesting the occurrence of cryptic self-incompatibility in this species. Cryptic self-incompatibility functioned differently in the two morphs, with pollen tube growth rates after legitimate and illegitimate pollination much more highly differentiated in pin flowers than in thrum flowers. No fruit was produced in emasculated netted flowers, suggesting the absence of apomixis. Our results indicate that H. acutangula is distylous, with a cryptic self-incompatibility breeding system. PMID:20522185

  16. The Construal (In)compatibility Effect: The Moderating Role of a Creative Mind-Set

    Xiaojing Yang; Torsten Ringberg; Huifang Mao; Laura A. Peracchio

    2011-01-01

    This research examines how consumers with a creative mind-set are persuaded by advertising claims construed at different levels (i.e., abstract vs. concrete ad claims). Across four experiments, we show that consumers with a creative mind-set are more persuaded by ad claims construed at a level incompatible with their mental construal, while ad claims construed at a level compatible with consumers’ mental construal are more effective for those who possess a less creative mind-set. We document ...

  17. Errors caused by incompatible wind and buoyancy forcing in the ocean general circulation models.

    Kuo, Yu-Heng

    1992-01-01

    Approved for public release; distribution is unlimited The Geophysical Fluid Dynamics Laboratory Modular Ocean Model (GFDL MOM) is used to investigate the model difference between compatible and incompatible surface wind and buoyancy forcing. The atmosphere is a physical system in which surface wind and temperature fields are related, however in most ocean numerical models, the wind stress and buoyancy forcing are usually specified separately, i.e., no constraint between the...

  18. Street-weighted interpolation techniques for demographic count estimation in incompatible zone systems

    Michael Reibel; Michael E Bufalino

    2005-01-01

    Data processing for the spatial analysis of small-area social, demographic, and economic data often requires the combination of data spatially aggregated to two or more incompatible zone systems in a region, such as a set of enumeration districts that changes over time. Such situations can be addressed by areal interpolation -- the transfer of data between zonal systems according to spatial algorithms. The authors test a technique of areal interpolation using geographic information systems (G...

  19. Molecular genetics, physiology and biology of self-incompatibility in Brassicaceae

    Watanabe, Masao; Suwabe, Keita; Suzuki, Go

    2012-01-01

    Self-incompatibility (SI) is defined as the inability to produce zygotes after self-pollination in a fertile hermaphrodite plant, which has stamens and pistils in the same flower. This structural organization of the hermaphrodite flower increases the risk of self-pollination, leading to low genetic diversity. To avoid this problem plants have established several pollination systems, among which the most elegant system is surely SI. The SI trait can be observed in Brassica crops, including cab...

  20. Effects of pollen reward removal on fecundity in a self-incompatible hermaphrodite plant.

    Duffy, K J; Johnson, S D

    2011-05-01

    The pollen of hermaphrodite plants is often utilised by flower-visiting animals. While pollen production has obvious benefits for plant male fitness, its consequences for plant female fitness, especially in self-incompatible hermaphrodite species, are less certain. Pollen production could either enhance seed production though increased pollinator attraction, or reduce it if ovules are discounted by deposition of self pollen, as can occur in species with late-acting self-incompatibility. To test the effects of pollen reward provision on female fitness, we artificially emasculated flowers in two populations of the succulent Aloe maculata (Asphodelaceae), which has a late-acting self-incompatibility system, over the course of its flowering period. Flowers of this species are visited by sunbirds (for nectar) and native bees (for pollen and nectar). We measured floral visitation rates, floral rejection rates, pollen deposition on stigmas and fruit and seed set in both emasculated and non-emasculated plants. We found that flowers of emasculated plants suffered reduced visitation and increased rejection (arrival without visitation) by bees, but not by sunbirds; had fewer pollen grains deposited on stigmas and showed an overall decrease in fruit set and seed set. Rates of seed abortion were, however, greatly reduced in emasculated flowers. This study shows that pollen rewards can be important for seed set, even in self-incompatible plants, which have been assumed to rely on nectar rewards for pollinator attraction. Seed abortion was, however, increased by pollen production, a result that highlights the complexity of selection on pollen production in hermaphrodite flowers. PMID:21489109

  1. Ascertaining complementary and incompatible quantum properties by means of double-slit experiments

    The famous two-slits experiment is used to theoretically introduce the problem of detecting both which-slit (WS) property and another quantum property incompatible with the WS one, together with the measurement of the (complementary) position of the final impact point. General conditions for the existence of solutions are singled out, and a family of solutions is concretely found. Moreover, we theoretically design an ideal experiment which realizes this non-trivial detection

  2. OFFSITE RADIOLOGICAL CONSEQUENCE CALCULATION FOR THE BOUNDING MIXING OF INCOMPATIBLE MATERIALS ACCIDENT

    This document quantifies the offsite radiological consequence of the bounding mixing of incompatible materials accident for comparison with the 25 rem Evaluation Guideline established in Appendix A of DOE-STD-3009. The bounding accident is an inadvertent addition of acid to a waste tank. The calculated offsite dose does not challenge the Evaluation Guideline. Revision 4 updates the analysis to consider bulk chemical additions to single shell tanks (SSTs)

  3. Crossability and evaluation of incompatibility barriers in crosses between Capsicum species

    Kellen Coutinho Martins; Telma Nair Santana Pereira; Sérgio Alessandro Machado Souza; Rosana Rodrigues; Antônio Teixeira do Amaral Junior

    2015-01-01

    The objectives of this study were to estimate the crossability rate in combinations of and assess the occurrence of incompatibility barriers between Capsicum species. The species C. annuum, C. chinense, C. frutescens, C. baccatum var. pendulum, and C. baccatum var. baccatum were crossed and the number of hand-pollinations and of resulting fruits and plants was registered. The resulting hybrids were evaluated for their pollen viability and in the crosses that produced no hybrids, the in vivo g...

  4. Linear viscoelasticity of incompatible polymer blends in the melt in relation with interfacial properties

    Graebling, D.; Muller, R.; Palierne, J.

    1993-01-01

    A quite general characteristic of the rheology of incompatible polymer blends in the melt is their highly elastic behaviour at low fiequencies, corresponding to long-time relaxation processes. For emulsions of Newtonian liquids, this property can be readily connected to interfacial tension α : in a macroscopic flow, suspended droplets of radius R are subjected on the one hand to a viscous drag related to the viscosity µm of the matrix liquid and tending to deform their shape, and on the other...

  5. Phase separation of weakly incompatible polymer blends confined in isolated droplets

    Müller-Buschbaum, P; Cubitt, R

    2002-01-01

    Phase separation of the weakly incompatible blend system polystyrene and polyparamethylstyrene confined in isolated droplets is investigated. The droplet geometry imposes a two-dimensional spatial restriction. With specular, diffuse and grazing incidence small-angle neutron scattering the surface topography as well as the chemical morphology inside the droplets is determined. Due to the differences in surface tension, a core-shell-like structure characterized by one most prominent length scale inside the droplets is installed. (orig.)

  6. Quantitative proteomics and transcriptomics of potato in response to Phytophthora infestans in compatible and incompatible interactions

    Ali, Ashfaq; Alexandersson, Erik; Sandin, Marianne; Resjö, Svante; Lenman, Marit; Hedley, Pete; Levander, Fredrik; Andreasson, Erik

    2014-01-01

    Background In order to get global molecular understanding of one of the most important crop diseases worldwide, we investigated compatible and incompatible interactions between Phytophthora infestans and potato (Solanum tuberosum). We used the two most field-resistant potato clones under Swedish growing conditions, which have the greatest known local diversity of P. infestans populations, and a reference compatible cultivar. Results Quantitative label-free proteomics of 51 apoplastic secretom...

  7. Declarations of incompatibility under the ECHR Act 2003 : a workable transplant?

    De Londras, Fiona

    2014-01-01

    Ireland’s European Convention on Human Rights (ECHR) Act 2003 has not had a very significant impact on domestic rights-related jurisprudence. This is, perhaps, not entirely surprising given that the Irish Constitution—Bunreacht na hÉireann—protects fundamental rights and that incompatibility with constitutional rights is fatal to legislation, with superior courts being constitutionally empowered to strike such law down. Thus, rather than a political constitution, Ireland has a ‘legal’ constit...

  8. Wolbachia-induced cytoplasmic incompatibility as a means for insect pest population control

    Zabalou, Sofia; Riegler, Markus; Theodorakopoulou, Marianna; STAUFFER, CHRISTIAN; Savakis, Charalambos; Bourtzis,Kostas

    2004-01-01

    Biological control is the purposeful introduction of parasites, predators, and pathogens to reduce or suppress pest populations. Wolbachia are inherited bacteria of arthropods that have recently attracted attention for their potential as new biocontrol agents. Wolbachia manipulate host reproduction by using several strategies, one of which is cytoplasmic incompatibility (CI) [Stouthamer, R., Breeuwer, J. A. J. & Hurst, G. D. D. (1999) Annu. Rev. Microbiol. 53, 71–102]. We established Wolbachi...

  9. Galaxy Evolution by the Incompatibility between Dark Matter and Baryonic Matter

    Ding-Yu Chung

    2014-01-01

    The paper derives the galaxy evolution by the non-interacting (incompatibility) between dark matter and baryonic matter in terms of the short-range separation between dark matter and baryonic matter, so dark matter cannot contact baryonic matter. In the conventional CDM (cold dark matter) model, dark matter and baryonic matter are interactive (compatible), so dark matter can contact baryonic matter. However, the conventional CDM model fails to account for the failure to...

  10. Non-ABO blood group systems phenotyping in non-human primates for blood banking laboratory and xenotransplantation.

    Ramis, G; Martínez-Alarcon, L; Quereda, J J; Mrowiec, A; Funes, C; Ríos, A; Ramírez, P; Muñoz, A; Majado, M J

    2013-04-01

    Some biomedical research procedures, such as organ xenotransplantation, usually require intensive hemotherapy. Knowledge of the whole phenotype of blood donor and graft could be useful in the field of xenotransplantation. Human and simian-type categories of blood groups have been established and they can be tested by standard methods used for human blood grouping. The aim of this work was to study the incidence of non-ABO blood group systems in different species of non-human primates, which are employed in biomedical research. The phenotype of Rh, Lewis, Kidd, Kell, MNSs, Lutheran, P and Duffy antigens was investigated in olive baboon (n = 48), chacma baboon (n = 9), Guinea baboon (n = 14), Rhesus macaque (n = 38) and squirrel monkey (n = 30) by using commercial microtyping cards. Kell, Lutheran, Kidd and Duffy antigens have been detected in all species, Rh in squirrel monkey, MNSs in rhesus macaque and squirrel monkey, and Lewis in baboon and rhesus macaque. There were differences in frequency and haemagglutination scores between species regardless of their gender and age. The main differences were found in squirrel monkey when compared with baboons and macaques. This typing system provides a tool to assess the presence of antigens in animals used for experimental procedures, such as xenotransplantation and xenotransfusion. PMID:23563364

  11. STUDY OF SEX RATIO, ABO AND Rh BLOOD GROUPS DISTRIBUTION IN SOME HAEMATOLOGICAL AND LYMPHATIC DISEASES IN IRAN

    D.D. Farhud

    1995-08-01

    Full Text Available Associations of some hematological and lymphatic diseases with sex and ABO and Rh blood groups were studied in 2579 patients, compared with a control group of 126332 individuals, by the use of clinical as well as laboratory findings, in Tehran. Highly significant increase of male/female ratio is shown in acute myelogenous leukemia, acute lymphocytic leukemia, chronic lymphocytic leukemia, aplastic anaemia and paroxismal nocturnal haematuria. Idiopathic thrombocy’topoenic purpura shows a decrease of this ration. In chronic myelocytic leukemia and acute lymphocytic leukemia, group 0 has the highest incidence, followed by groups A and B, and group AB has the lowest incidence. Between various diseases, the highest frequency of blood group A was went observed in Hodgkin’s disease, and the lowest in NHL. Group B had highest frequency within PNH patients and the lowest in NHL. Group 0 had the highest In AML patients there is a significant decrease of Rh negative patient 17.15%. The highest incidence of Rh negative was among the SA patients (18.18% and the lowest in MM (3.70%.

  12. Genome-wide association study identifies variants in the ABO locus associated with susceptibility to pancreatic cancer

    Amundadottir, Laufey; Kraft, Peter; Stolzenberg-Solomon, Rachael Z.; Fuchs, Charles S.; Petersen, Gloria M.; Arslan, Alan A.; Bueno-de-Mesquita, H. Bas; Gross, Myron; Helzlsouer, Kathy; Jacobs, Eric J.; LaCroix, Andrea; Zheng, Wei; Albanes, Demetrius; Bamlet, William; Berg, Christine D.; Berrino, Franco; Bingham, Sheila; Buring, Julie E.; Bracci, Paige M.; Canzian, Federico; Clavel-Chapelon, Françoise; Clipp, Sandra; Cotterchio, Michelle; de Andrade, Mariza; Duell, Eric J.; Fox, John W.; Gallinger, Steven; Gaziano, J. Michael; Giovannucci, Edward L.; Goggins, Michael; González, Carlos A.; Hallmans, Göran; Hankinson, Susan E.; Hassan, Manal; Holly, Elizabeth A.; Hunter, David J.; Hutchinson, Amy; Jackson, Rebecca; Jacobs, Kevin B.; Jenab, Mazda; Kaaks, Rudolf; Klein, Alison P.; Kooperberg, Charles; Kurtz, Robert C.; Li, Donghui; Lynch, Shannon M.; Mandelson, Margaret; McWilliams, Robert R.; Mendelsohn, Julie B.; Michaud, Dominique S.; Olson, Sara H.; Overvad, Kim; Patel, Alpa V.; Peeters, Petra H.M.; Rajkovic, Aleksandar; Riboli, Elio; Risch, Harvey A.; Shu, Xiao-Ou; Thomas, Gilles; Tobias, Geoffrey S.; Trichopoulos, Dimitrios; Van Den Eeden, Stephen K.; Virtamo, Jarmo; Wactawski-Wende, Jean; Wolpin, Brian M.; Yu, Herbert; Yu, Kai; Zeleniuch-Jacquotte, Anne; Chanock, Stephen J.; Hartge, Patricia; Hoover, Robert N.

    2010-01-01

    We conducted a two-stage genome-wide association study (GWAS) of pancreatic cancer, a cancer with one of the poorest survival rates worldwide. Initially, we genotyped 558,542 single nucleotide polymorphisms in 1,896 incident cases and 1,939 controls drawn from twelve prospective cohorts plus one hospital-based case-control study. In a combined analysis adjusted for study, sex, ancestry and five principal components that included an additional 2,457 cases and 2,654 controls from eight case-control studies, we identified an association between a locus on 9q34 and pancreatic cancer marked by the single nucleotide polymorphism, rs505922 (combined P=5.37 × 10-8; multiplicative per-allele odds ratio (OR) 1.20; 95% CI 1.12-1.28). This SNP maps to the first intron of the ABO blood group gene. Our results are consistent with earlier epidemiologic evidence suggesting that people with blood group O may have a lower risk of pancreatic cancer than those with groups A or B. PMID:19648918

  13. Curing of plasmid pXO1 from Bacillus anthracis using plasmid incompatibility.

    Xiankai Liu

    Full Text Available The large plasmid pXO1 encoding the anthrax toxin is important for the virulence of Bacillus anthracis. It is essential to cure pXO1 from B. anthracis to evaluate its role in the pathogenesis of anthrax infection. Because conventional methods for curing plasmids (e.g., curing agents or growth at elevated temperatures can induce mutations in the host chromosomal DNA, we developed a specific and reliable method to eliminate pXO1 from B. anthracis using plasmid incompatibility. Three putative replication origins of pXO1 were inserted into a temperature-sensitive plasmid to generate three incompatible plasmids. One of the three plasmids successfully eliminated the large plasmid pXO1 from B. anthracis vaccine strain A16R and wild type strain A16. These findings provided additional information about the replication/partitioning of pXO1 and demonstrated that introducing a small incompatible plasmid can generate plasmid-cured strains of B. anthracis without inducing spontaneous mutations in the host chromosome.

  14. Risk estimation of HNA-3 incompatibility and alloimmunization in Thai populations.

    Nathalang, Oytip; Intharanut, Kamphon; Siriphanthong, Kanokpol; Nathalang, Siriporn; Leetrakool, Nipapan

    2015-01-01

    Severe transfusion-related acute lung injury (TRALI) is often due to antibodies in blood components directed against human neutrophil antigen (HNA)-3a. This study aimed to report the genotype frequencies of the HNA-3 system and to estimate the potential risk of HNA-3 incompatibility and alloimmunization in two Thai populations. Eight hundred DNA samples obtained from 500 unrelated healthy blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok and 300 samples from the Blood Bank, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand were included. HNA-3 genotyping was performed using an in-house polymerase chain reaction with sequence-specific primer (PCR-SSP) technique. The observed frequencies of the HNA-3a/3a, HNA-3a/3b, and HNA-3b/3b genotypes were 0.528, 0.380, and 0.092 in central Thais and 0.600, 0.350, and 0.050 in northern Thais, respectively. The frequencies were used to estimate HNA-3 incompatibility and risk of HNA-3a alloimmunization. The HNA-3 incompatibility in central Thais (33.28%) was higher than northern Thais (28.75%), corresponding to a significantly higher probability of HNA-3a alloimmunization (Ptransfusion. PMID:25608003

  15. Rh Incompatibility

    ... Blood and Marrow Stem Cell Transplant Heart Failure Hemolytic Anemia Send a link to NHLBI to someone by ... baby's red blood cells. This can lead to hemolytic anemia (HEE-moh-lit-ick uh-NEE-me-uh) ...

  16. Phoenix : Complex Adaptive System of Systems (CASoS) engineering version 1.0.

    Moore, Thomas W.; Quach, Tu-Thach; Detry, Richard Joseph; Conrad, Stephen Hamilton; Kelic, Andjelka; Starks, Shirley J.; Beyeler, Walter Eugene; Brodsky, Nancy S.; Verzi, Stephen J.; Brown, Theresa Jean; Glass, Robert John, Jr.; Sunderland, Daniel J.; Mitchell, Michael David; Ames, Arlo Leroy; Maffitt, S. Louise; Finley, Patrick D.; Russell, Eric Dean; Zagonel, Aldo A.; Reedy, Geoffrey E.; Mitchell, Roger A.; Corbet, Thomas Frank, Jr.; Linebarger, John Michael

    2011-08-01

    Complex Adaptive Systems of Systems, or CASoS, are vastly complex ecological, sociological, economic and/or technical systems which we must understand to design a secure future for the nation and the world. Perturbations/disruptions in CASoS have the potential for far-reaching effects due to pervasive interdependencies and attendant vulnerabilities to cascades in associated systems. Phoenix was initiated to address this high-impact problem space as engineers. Our overarching goals are maximizing security, maximizing health, and minimizing risk. We design interventions, or problem solutions, that influence CASoS to achieve specific aspirations. Through application to real-world problems, Phoenix is evolving the principles and discipline of CASoS Engineering while growing a community of practice and the CASoS engineers to populate it. Both grounded in reality and working to extend our understanding and control of that reality, Phoenix is at the same time a solution within a CASoS and a CASoS itself.

  17. Complex Adaptive System of Systems (CASoS) Engineering Applications. Version 1.0.

    Linebarger, John Michael; Maffitt, S. Louise (New Mexico Institute of Mining and Technology, Albuquerque, NM); Glass, Robert John, Jr.; Beyeler, Walter Eugene; Brown, Theresa Jean; Ames, Arlo Leroy

    2011-10-01

    Complex Adaptive Systems of Systems, or CASoS, are vastly complex eco-socio-economic-technical systems which we must understand to design a secure future for the nation and the world. Perturbations/disruptions in CASoS have the potential for far-reaching effects due to highly-saturated interdependencies and allied vulnerabilities to cascades in associated systems. The Phoenix initiative approaches this high-impact problem space as engineers, devising interventions (problem solutions) that influence CASoS to achieve specific aspirations. CASoS embody the world's biggest problems and greatest opportunities: applications to real world problems are the driving force of our effort. We are developing engineering theory and practice together to create a discipline that is grounded in reality, extends our understanding of how CASoS behave, and allows us to better control those behaviors. Through application to real-world problems, Phoenix is evolving CASoS Engineering principles while growing a community of practice and the CASoS engineers to populate it.

  18. Complex Adaptive Systems of Systems (CASoS) engineering and foundations for global design.

    Brodsky, Nancy S.; Finley, Patrick D.; Beyeler, Walter Eugene; Brown, Theresa Jean; Linebarger, John Michael; Moore, Thomas W.; Glass, Robert John, Jr.; Maffitt, S. Louise; Mitchell, Michael David; Ames, Arlo Leroy

    2012-01-01

    Complex Adaptive Systems of Systems, or CASoS, are vastly complex ecological, sociological, economic and/or technical systems which must be recognized and reckoned with to design a secure future for the nation and the world. Design within CASoS requires the fostering of a new discipline, CASoS Engineering, and the building of capability to support it. Towards this primary objective, we created the Phoenix Pilot as a crucible from which systemization of the new discipline could emerge. Using a wide range of applications, Phoenix has begun building both theoretical foundations and capability for: the integration of Applications to continuously build common understanding and capability; a Framework for defining problems, designing and testing solutions, and actualizing these solutions within the CASoS of interest; and an engineering Environment required for 'the doing' of CASoS Engineering. In a secondary objective, we applied CASoS Engineering principles to begin to build a foundation for design in context of Global CASoS

  19. Cytological aspects of incompatible and compatible interactions between rice, wheat and the blast pathogen Pyricularia oryzae

    Leonardo Araujo

    2016-04-01

    Full Text Available ABSTRACT Blast, caused by the fungus Pyricularia oryzae, is an important disease affecting rice and wheat yield worldwide. This study investigated the cytological aspects of incompatible (non-host resistance and compatible (host resistance rice- (R_Po and wheat- (W_Po Pyricularia oryzae isolate interactions. Inoculations of rice and wheat with the R_Po and W_Po isolates of P. oryzae, respectively, were expected to be compatible interactions (host resistance, whereas inoculations of rice and wheat with the W_Po and R_Po isolates of P. oryzae, respectively, were considered to be incompatible interactions (non-host resistance. For the compatible interactions (rice-R_Po and wheat-W_Po, fungal hyphae penetrated and colonized the epidermal cells and also invaded many neighboring cells. By contrast, in the case of the incompatible interactions (rice-W_Po and wheat-R_Po, fungal hyphae were not able to penetrate nor colonize the epidermal cells, but when penetration did occur, the hyphae were restricted to the first-invaded epidermal cell. The frequency of appressorial sites exhibiting infection hyphae within the epidermal cell underlying an appressorium was greater in the case of the compatible interactions. By contrast, unsuccessful penetrations with cytoplasmic granulation occurred with high frequency in the incompatible wheat-R_Po and rice-W_Po interactions and the number of necrotic epidermal cells underlying the appressorium was low for the rice-W_Po interaction as well as for the wheat- R_Po interaction, where no symptoms of necrosis were exhibited. However, the opposite was observed for the compatible interactions. The present study presents cytological features associated with incompatible and compatible rice- and wheat-P. oryzae interactions that may be useful to studies involving variability, coevolution, diagnosis, and regulation of quarantine or even in a rice or wheat breeding program whose aim is to transfer genes involved in non

  20. Mutability of the self-incompatibility locus and identification of the S-bearing chromosome in Nicotiana alata

    γ rays, X rays, fast neutrons and ethyl methanesulfonate (EMS) were used for inducing mutations at the self-incompatibility locus of Nicotiana alata. Chronic gamma irradiation and EMS treatment neither induced self-compatability mutations nor led to changes from one S allele to another. X rays and fast neutrons induced many self-compatibility mutations, but did not generate new self-incompatibility alleles. (Auth.)

  1. A low diversity, seasonal tropical landscape dominated by conifers and peltasperms: Early Permian Abo Formation, New Mexico

    DiMichele, W.A.; Chaney, D.S.; Nelson, W.J.; Lucas, S.G.; Looy, C.V.; Quick, K.; Jun, W.

    2007-01-01

    Walchian conifers (Walchia piniformis Sternberg, 1825) and peltasperms similar to Supaia thinnfeldioides White and cf. Supaia anomala White dominate floodplain deposits of a narrow stratigraphic interval of the middle Abo Formation, Lower Permian of central New Mexico. The plant fossils occur in thinly bedded units up to two meters thick, consisting of coarse siltstone to very fine sandstone with clay partings. Bedding is primarily tabular, thin, and bears rare ripple marks and trough cross beds. Bedding surfaces display mud cracks, raindrop imprints, horizontal and vertical burrows of invertebrates, and footprints of terrestrial vertebrates. These features indicate intermittent and generally unchannelized stream flow, with repeated exposure to air. Channels appear to have cannibalized one another on a slowly subsiding coastal plain. Conifers are dominant at three collecting sites and at three others Supaia dominates. Although each of these genera occurs in assemblages dominated by the other, there are no truly co-dominant assemblages. This pattern suggests alternative explanations. Landscapes could have consisted of a small-scale vegetational patchwork dominated almost monospecifically in any one patch, meaning that these plants could have coexisted across the landscape. On the other hand, conifer and supaioid dominance could have been temporally distinct, occurring during different episodes of sedimentation; although in the field there are no noticeable sedimentological differences between conifer-dominated and Supaia-dominated channel deposits, they may represent slightly different climatic regimes. The considerable morphological differences between conifers and Supaia suggest that the floristic patterns are not a taphonomic effect of the loss of a significant part of the original biodiversity. In general, the climate under which this vegetation developed appears to have been relatively warm and arid, based on the geology (pervasive red color [oxidation

  2. FREQUENCY AND DISTRIBUTION OF ABO & RH BLOOD GROUP IN BILASPUR DISTRICT OF CHHATTISGARH STATE : A STUDY FROM MEDICAL COLLEGE HOSPITAL

    Bhanu Pratap

    2015-07-01

    Full Text Available BACKGROUND : Approximate 30 blood group systems have discovered and more than 400 erythrocytes antigens are identified. Blood group ABO and Rh are most important among all other blood group systems in transfusion service practices. The frequency of four major blood gr oup s namely A, B, O, AB with Rh Positive and Negative varies in different population of the world and differ also in region and race wise. MATERIAL AND METHOD : This 5 years retrospective study was conducted at Blood Bank of a Medical college Hospital of Bi laspur in Northern Chhattisgarh, catering the 1/3 population of state. Data were collected from the Blood Bank Grouping record from the period of January 2010 to December 2014. Blood group of blood donors and patients were determined by Monoclonal Anti Ser a by slide agglutinations tests. Rare case and difficult case were examined by test tube agglutination method and Matrix Gel System of Tulip. RESULT AND CONCLUSIO N: 31973 subjects were examined for blood group during observation period, Out of these 31092( 97.25% were male and 881 (2.75% were female. The frequency of blood group B in these populations was 11007 (34.42% (33.36% Rh Positive and 1.06% Rh Negative Followed by O were 10864 (33.97% (33.33% Rh Positive and 0.64% Rh Negative, A was 9113 (28.50 % (27.99 % Rh Positive and 0.51% Rh Negative and AB was 989 (3.11% (3.01% Rh Positive and 0.1% Rh Negative. Rhesus group Rh Positive were 31242 (97.7 % and Rh Negative were 731 (2.3 %.

  3. The Study of ABO Groups and Rh Factor in Active and Non-active Carriers of Hepatitis B Virus

    Haydeh Alaoddolehei et al.

    2007-01-01

    Full Text Available During past eight decades, many studies have been performed to determine relationship between infectious diseases and blood groups. Interaction of microorganisms and RBC membrane is probably because of antigenic similarity, adherence through specific receptors or demodulation of antibody response (1. The first known relationship between blood groups and infectious diseases was seen in Plasmodium vivax. It is believed that sensitivity to HIV infection is related to blood groups and Rh factor (2. Hepatitis is a general word which caused by many factors such as DNA virus named HBV (hepatitis B virus. Several serologic determinants [eg. Glycoprotein surface antigen (HBsAg, viral peptide antigen (HBeAg, antibody against viral nucleoprotein (HBcAb] and PCR lead to recognition of HBV (3. A number of individuals with chronic infection (presence of HBsAg are divided to active and onactive groups. All cases are positive in HBcAb and negative in HBsAb. Active cases are recognized by detection of HBeAg and HBV-DNA, some clinical symptoms and elevated laboratory tests (ALT and AST (4. This study was performed based on presence of ABO and Rh antigens on other cells (5 which could be used as receptors for viruses. All individuals infected with HBV in the past or present time who were referred to a clinic were enrolled to this study. Fifty-five patients (10 female and 45 male were active and 182 (64 female and118 male were non-active out of 237 person with HBsAg. The blood samples were collected and ABO and Rh typing was done by Blood Filtration and Investigation Co (Palayesh and Pajohesh of blood company. Lot. No: MAbA09 antiserum. Data were analyzed by SPSS software using ||2 test. This study showed that active individuals have A (18.2%, B (18.2%, O (58.2%, AB (5.4%, Rh positive (96.4% and Rh negative (3.6% and nonactive individuals have A (26.9%, B (25.3%, O (41.2%, AB (6.6%, Rh positive (98.4% and Rh negative (1.6% (Table 1. These findings revealed that

  4. FREQUENCY OF ABO AND RHESUS (D BLOOD GROUPS IN TRIBAL P OPULATION OF MADHYA PRADESH: A STUDY FROM TERTIARY CARE TEACHING HOSPITAL IN MADHYA PRADESH

    Ajit

    2015-04-01

    Full Text Available BACKGROUND: ABO and Rh blood groups are most important blood groups in human beings. The frequency of four main blood group systems varies in population throughout the world and even in different parts of country. Objective if this study was to identify distribution o f ABO and Rh blood group system. MATERIALS AND METHODS: Blood samples from 10680 tribals were collected in Jhabua district of Madhya Pradesh during the month of June 2012. Among 10680 tribals, 5670 were male. Blood groups were done in tribals belonging to Bhil, Bhilala & Katthiwar tribes. For the blood grouping of the patients, 5cc of clotted blood was collected & transported to Department Pathology, Peoples College of medical sciences, Bhopal . RESULTS : A total 10680 samples were analyzed, out of which 5670 (53% samples were of male. The frequency of blood group B in our population was 36 . 9 %; n= 3950 (35 . 37% B Rh positive and 1 . 61% B Rh negative and frequency of blood group B remain highest in our study group . The frequency of blood group O in our populat ion was 31 . 8%; n=3400 (30 . 43% O Rh positive and 1 . 4% O Rh negative followed by blood group A was 24 . 3%; n=2600 (23 . 15% A Rh positive and 1 . 18% A Rh negative and blood group AB was 6 . 8%; n=730 (6 . 63% AB Rh positive and 0 . 2% AB Rh negative The overall phe notypic frequencies of ABO blood groups were B>O>A>AB . Rh (D positive were 95 . 59%; n=10210 and Rh (D negative were 4 . 41%; n=470 . DISCUSSION: B positive blood group is significantly high in our population. Every transfusion center should have a record of frequency of blood group system in their population. It helps in inventory management. Knowledge of blood group distribution is important for clinical studies, for reliable geographical information and for forensic studies in the population.

  5. Adding perches for cross-pollination ensures the reproduction of a self-incompatible orchid.

    Zhong-Jian Liu

    Full Text Available BACKGROUND: Outcrossing is known to carry genetic advantages in comparison with inbreeding. In many cases, flowering plants develop a self-incompatibility mechanism, along with a floral component adaptation mechanism, to avoid self-pollination and to promote outbreeding. Orchids commonly have a lip in their flower that functions as the a visiting plate for insect pollinators. Aside from the lip, however, many species (including Coelogyne rigida have sheaths around the axis of inflorescence. The function of these sheaths remains unknown, and has long been a puzzle to researchers. METHODOLOGY/PRINCIPAL FINDINGS: We investigated the function of these sheaths in relation to the lip and the pollinators, as well as their role in the modes of pollination and reproduction of Coelogyne rigida in 30 flowering populations of orchids in the limestone area of Southeast Yunnan, China. We found that self-incompatible C. rigida developed specialized bird perches around the basal axis of inflorescence to attract sunbirds and to complement their behavioral tendency to change foraging locations frequently. This self-incompatibility mechanism operates separately from the floral component adaptation mechanism. This mechanism thus prevents bees from repeatedly visiting the floral lip of the same plant which, in turn, results in autogamy. In this way, instead of preventing autogamy, C. rigida responds to these negative effects through a highly efficient cross-pollination method that successfully transfers pollen to different plants. CONCLUSIONS: The proposed method ensures reproductive success, while offsetting the infertile self-pollination by insects, thereby reducing mating costs and addressing the lack of cross-pollination. The adaptation provides a novel and striking example of structural adaptation that promotes cross-pollination in angiosperms.

  6. ABO histo-blood groups and Rh systems in relation to malignant tumors of the digestive tract in Bosnia and Herzegovina

    Jovanović-Ćupić Snežana

    2008-01-01

    Full Text Available The distribution of ABO blood groups and the Rhesus factor was analyzed in 279 patients who suffered from malignant tumors of the digestive system. Patients were registered retrospectively in the Gastroenterohepatology Clinic, Clinical Center, University of Sarajevo over a discontinuous period of 88 months. From the results obtained, it was concluded that: (a men became ill from gastric cancer significantly more frequently than women; (b the frequency of liver carcinoma was three times higher than the global frequency and the frequency neighboring ethnic groups; and (c patients with blood group B and patients with RhD(- exhibited a significantly higher proportion of disease.

  7. The study of Allelic Frequency of ABO and Rh D Blood Group among the Banjara Population of Akola District, Maharashtra, India

    Aravind Chavhan

    2012-12-01

    Full Text Available The distribution of ABO blood groups and Rh (D factor has been studied in the Banjara population. In the present study O, A, B, and AB blood group percentages of Banjaras of Akola district of Maharashtra are recorded as 27.64%, 22.91%, 37.45% and 12% respectively and the Rh negative incidences recorded as 02.55%. The allelic frequencies of O, A, B and AB groups in the combined data of same community found to be 0.5196, 0.2880, and 0.1924 respectively and Rh (D positive as 0.8405.

  8. A variant in the ABO gene explains the variation in soluble E-selectin levels-results from dense genotyping in two independent populations.

    Mahir Karakas

    Full Text Available BACKGROUND: Elevated soluble (s E-selectin levels have been associated with various cardiovascular diseases. Recently, genetic variants in the ABO blood group have been related to E-selectin levels in a small cohort of patients with type 1 diabetes. We evaluated whether this association is reproducible in two large samples of Caucasians. METHODOLOGY/ PRINCIPAL FINDINGS: Data of the present study was drawn from the population-based MONICA/KORA Augsburg study (n = 1,482 and the patients-based LURIC study (n = 1,546. A high-density genotyping array (50K IBC Chip containing single-nucleotide polymorphisms (SNPs from E-selectin candidate genes selected on known biology of E-selectin metabolism, mouse genetic studies, and human genetic association studies, was used for genotyping. Linear regression analyses with adjustment for age and sex (and survey in KORA were applied to assess associations between gene variants and sE-selectin concentrations. A number of 12 SNPs (in KORA and 13 SNPs (in LURIC, all from the ABO blood group gene, were significantly associated with the log-transformed concentration of E-selectin. The strongest association was observed for rs651007 with a change of log-transformed sE-selectin per one copy of the minor allele of -0.37 ng/ml (p = 1.87×10(-103 in KORA and -0.35 ng/ml (p = 5.11×10(-84 in LURIC. Inclusion of rs651007 increased the explained sE-selectin variance by 0.256 in KORA and 0.213 in LURIC. All SNPs had minor allele frequencies above 20% showing a substantial gene variation. CONCLUSIONS/ SIGNIFICANCE: Our findings in two independent samples indicate that the genetic variants at the ABO locus affect sE-selectin levels. Since distinct genome-wide association studies linked the ABO gene with myocardial infarction (MI in the presence of coronary atherosclerosis and with coronary artery disease, these findings may not only enhance our understanding of adhesion molecule biology, but may also provide a focus for several

  9. La tesis del caso especial

    Alexy, Robert

    1999-11-01

    Full Text Available Not available

    A partir de la comprensión de la tesis del caso especial como un intento por dar cuenta de la doble vertiente de la argumentación jurídica (su carácter autoritativo, institucional o real, por un lado, y su dimensión libre, discursiva o ideal, por otro Alexy se propone defender esta tesis frente a las críticas tomadas en consideración o realizadas por Jürgen Habermas en su libro Facticidad y validez. En primer lugar, Alexy defiende su tesis frente a la crítica que sostiene que las limitaciones que existen en los procedimientos judiciales excluirían su comprensión en términos de teoría del discurso. En segundo lugar, el autor hace hincapié en que la tesis del caso especial que él defiende implica que la argumentación jurídica ha de entenderse como un caso especial del discurso práctico general y no meramente del discurso moral tal y como es definido por Habermas. Y por último, defiende la tesis del caso especial contra la objeción de que los argumentos prácticos generales cambiarían su carácter al pasar a formar parte del Derecho; para ello propugna concebir de manera procedimental la integración de los argumentos prácticos generales en el contexto jurídico.

  10. Clinical Questions of Tissue Incompatibility after Allogenic Bone-Marrow Transplantation

    This paper describes the results of studies concerning tissue incompatibility in the transplantation of allogenic bone marrow into patients suffering from hypoplastic and aplastic anaemia. Factual data are presented on the extent to which the immune activity and capacity for immunological response of recipients is preserved. Attention is mainly directed to the characteristics of the spectrum showing serological activity of the anti-leucocyte antibodies, which depends on the type of sensitization. These data point to the need for differential use of haemotherapeutic agents and are also of some importance in the selection of bone-marrow donors. (author)

  11. Wolbachia symbiont infections induce strong cytoplasmic incompatibility in the tsetse fly Glossina morsitans.

    Uzma Alam

    2011-12-01

    Full Text Available Tsetse flies are vectors of the protozoan parasite African trypanosomes, which cause sleeping sickness disease in humans and nagana in livestock. Although there are no effective vaccines and efficacious drugs against this parasite, vector reduction methods have been successful in curbing the disease, especially for nagana. Potential vector control methods that do not involve use of chemicals is a genetic modification approach where flies engineered to be parasite resistant are allowed to replace their susceptible natural counterparts, and Sterile Insect technique (SIT where males sterilized by chemical means are released to suppress female fecundity. The success of genetic modification approaches requires identification of strong drive systems to spread the desirable traits and the efficacy of SIT can be enhanced by identification of natural mating incompatibility. One such drive mechanism results from the cytoplasmic incompatibility (CI phenomenon induced by the symbiont Wolbachia. CI can also be used to induce natural mating incompatibility between release males and natural populations. Although Wolbachia infections have been reported in tsetse, it has been a challenge to understand their functional biology as attempts to cure tsetse of Wolbachia infections by antibiotic treatment damages the obligate mutualistic symbiont (Wigglesworthia, without which the flies are sterile. Here, we developed aposymbiotic (symbiont-free and fertile tsetse lines by dietary provisioning of tetracycline supplemented blood meals with yeast extract, which rescues Wigglesworthia-induced sterility. Our results reveal that Wolbachia infections confer strong CI during embryogenesis in Wolbachia-free (Gmm(Apo females when mated with Wolbachia-infected (Gmm(Wt males. These results are the first demonstration of the biological significance of Wolbachia infections in tsetse. Furthermore, when incorporated into a mathematical model, our results confirm that Wolbachia can

  12. Enterococcus faecalis hemolysin-bacteriocin plasmids belong to the same incompatibility group.

    Colmar, I; Horaud, T

    1987-01-01

    Plasmid pair coexistence was studied both among nine Enterococcus faecalis hemolysin-bacteriocin (Hly-Bcn) plasmids, including pJH2, pAD1, pAM gamma 1, and pIP964, and between pIP964 and five R plasmids. Some of the Hly-Bcn plasmids used were derivatives encoding resistance to erythromycin or tetracycline. The Hly-Bcn plasmids were incompatible with each other; 40 to 100% displacement was observed bilaterally for eight pairs and unilaterally for one pair. In contrast, pIP964 stably coexisted ...

  13. Interallelic and intergenic incompatibilities of the Prdm9 (Hst1 gene in mouse hybrid sterility.

    Petr Flachs

    Full Text Available The Dobzhansky-Muller model of incompatibilities explains reproductive isolation between species by incorrect epistatic interactions. Although the mechanisms of speciation are of great interest, no incompatibility has been characterized at the gene level in mammals. The Hybrid sterility 1 gene (Hst1 participates in the arrest of meiosis in F(1 males of certain strains from two Mus musculus subspecies, e.g., PWD from M. m. musculus and C57BL/6J (henceforth B6 from M. m. domesticus. Hst1 has been identified as a meiotic PR-domain gene (Prdm9 encoding histone 3 methyltransferase in the male offspring of PWD females and B6 males, (PWD×B6F(1. To characterize the incompatibilities underlying hybrid sterility, we phenotyped reproductive and meiotic markers in males with altered copy numbers of Prdm9. A partial rescue of fertility was observed upon removal of the B6 allele of Prdm9 from the azoospermic (PWD×B6F(1 hybrids, whereas removing one of the two Prdm9 copies in PWD or B6 background had no effect on male reproduction. Incompatibility(ies not involving Prdm9(B6 also acts in the (PWD×B6F(1 hybrids, since the correction of hybrid sterility by Prdm9(B6 deletion was not complete. Additions and subtractions of Prdm9 copies, as well as allelic replacements, improved meiotic progression and fecundity also in the progeny-producing reciprocal (B6×PWDF(1 males. Moreover, an increased dosage of Prdm9 and reciprocal cross enhanced fertility of other sperm-carrying male hybrids, (PWD×B6-C3H.Prdm9F(1, harboring another Prdm9 allele of M. m. domesticus origin. The levels of Prdm9 mRNA isoforms were similar in the prepubertal testes of all types of F(1 hybrids of PWD with B6 and B6-C3H.Prdm9 despite their different prospective fertility, but decreased to 53% after removal of Prdm9(B6. Therefore, the Prdm9(B6 allele probably takes part in posttranscriptional dominant-negative hybrid interaction(s absent in the parental strains.

  14. Wolbachia symbiont infections induce strong cytoplasmic incompatibility in the tsetse fly Glossina morsitans.

    Alam, Uzma; Medlock, Jan; Brelsfoard, Corey; Pais, Roshan; Lohs, Claudia; Balmand, Séverine; Carnogursky, Jozef; Heddi, Abdelaziz; Takac, Peter; Galvani, Alison; Aksoy, Serap

    2011-12-01

    Tsetse flies are vectors of the protozoan parasite African trypanosomes, which cause sleeping sickness disease in humans and nagana in livestock. Although there are no effective vaccines and efficacious drugs against this parasite, vector reduction methods have been successful in curbing the disease, especially for nagana. Potential vector control methods that do not involve use of chemicals is a genetic modification approach where flies engineered to be parasite resistant are allowed to replace their susceptible natural counterparts, and Sterile Insect technique (SIT) where males sterilized by chemical means are released to suppress female fecundity. The success of genetic modification approaches requires identification of strong drive systems to spread the desirable traits and the efficacy of SIT can be enhanced by identification of natural mating incompatibility. One such drive mechanism results from the cytoplasmic incompatibility (CI) phenomenon induced by the symbiont Wolbachia. CI can also be used to induce natural mating incompatibility between release males and natural populations. Although Wolbachia infections have been reported in tsetse, it has been a challenge to understand their functional biology as attempts to cure tsetse of Wolbachia infections by antibiotic treatment damages the obligate mutualistic symbiont (Wigglesworthia), without which the flies are sterile. Here, we developed aposymbiotic (symbiont-free) and fertile tsetse lines by dietary provisioning of tetracycline supplemented blood meals with yeast extract, which rescues Wigglesworthia-induced sterility. Our results reveal that Wolbachia infections confer strong CI during embryogenesis in Wolbachia-free (Gmm(Apo)) females when mated with Wolbachia-infected (Gmm(Wt)) males. These results are the first demonstration of the biological significance of Wolbachia infections in tsetse. Furthermore, when incorporated into a mathematical model, our results confirm that Wolbachia can be used

  15. Interallelic and intergenic incompatibilities of the Prdm9 (Hst1) gene in mouse hybrid sterility

    Flachs, Petr; Mihola, Ondřej; Šimeček, Petr; Gregorová, Soňa; Schimenti, J.C.; Matsui, Y.; Baudat, F.; de Massy, B.; Piálek, Jaroslav; Forejt, Jiří; Trachtulec, Zdeněk

    2012-01-01

    Roč. 8, č. 11 (2012), e1003044. ISSN 1553-7404 R&D Projects: GA MŠk(CZ) 1M0520; GA ČR GAP305/10/1931; GA ČR GA206/08/0640; GA ČR(CZ) GPP305/11/P630 Institutional research plan: CEZ:AV0Z50520514 Institutional support: RVO:68081766 ; RVO:68378050 Keywords : hybrid sterility * meiosis * incompatibility Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 8.517, year: 2012

  16. Incompatibility of the observer-based vacuum with canonical quantum field theory

    Three new stationary coordinate systems in flat space-time, the last of six possible types, are described. The vacuum state found by canonical quantization of the scalar field in two of these systems is identical to that of Rindler coordinates, yet detectors at rest in these systems are excited in different amounts. A definition of the vacuum based on these detectors is, therefore, incompatible with canonical quantum field theory. The vacuum of the third system is identical to the Minkowski coordinate vacuum; thus, there are only two different vacua in flat space-time

  17. Foco epidémico de sirenomelia en Cali, Colombia. Informe de 4 casos en el Hospital Universitario del Valle en 54 días

    Armicson Felipe Solano; Wilmar Saldarriaga; Carolina Isaza; Pierpaolo Mastroiacovo; Castilla, Eduardo E.

    2006-01-01

    La sirenomelia es una malformación congénita rara que se caracteriza por fusión de los miembros inferiores, asociada con malformaciones viscerales que pueden ser incompatibles con la vida. La etiología se desconoce. Se informa la prevalencia entre 1 y 2 en 100,000 nacimientos. Se presentan 4 casos de sirenomelia en niños recién nacidos en el Hospital Universitario del Valle de Cali, Colombia entre el 9 de diciembre de 2004 y el 2 de febrero de 2005, lo que correspondería a una prevalencia de ...

  18. Analysis of a plant complex resistance gene locus underlying immune-related hybrid incompatibility and its occurrence in nature.

    Alcázar, Rubén; von Reth, Marcel; Bautor, Jaqueline; Chae, Eunyoung; Weigel, Detlef; Koornneef, Maarten; Parker, Jane E

    2014-12-01

    Mechanisms underlying speciation in plants include detrimental (incompatible) genetic interactions between parental alleles that incur a fitness cost in hybrids. We reported on recessive hybrid incompatibility between an Arabidopsis thaliana strain from Poland, Landsberg erecta (Ler), and many Central Asian A. thaliana strains. The incompatible interaction is determined by a polymorphic cluster of Toll/interleukin-1 receptor-nucleotide binding-leucine rich repeat (TNL) RPP1 (Recognition of Peronospora parasitica1)-like genes in Ler and alleles of the receptor-like kinase Strubbelig Receptor Family 3 (SRF3) in Central Asian strains Kas-2 or Kond, causing temperature-dependent autoimmunity and loss of growth and reproductive fitness. Here, we genetically dissected the RPP1-like Ler locus to determine contributions of individual RPP1-like Ler (R1-R8) genes to the incompatibility. In a neutral background, expression of most RPP1-like Ler genes, except R3, has no effect on growth or pathogen resistance. Incompatibility involves increased R3 expression and engineered R3 overexpression in a neutral background induces dwarfism and sterility. However, no individual RPP1-like Ler gene is sufficient for incompatibility between Ler and Kas-2 or Kond, suggesting that co-action of at least two RPP1-like members underlies this epistatic interaction. We find that the RPP1-like Ler haplotype is frequent and occurs with other Ler RPP1-like alleles in a local population in Gorzów Wielkopolski (Poland). Only Gorzów individuals carrying the RPP1-like Ler haplotype are incompatible with Kas-2 and Kond, whereas other RPP1-like alleles in the population are compatible. Therefore, the RPP1-like Ler haplotype has been maintained in genetically different individuals at a single site, allowing exploration of forces shaping the evolution of RPP1-like genes at local and regional population scales. PMID:25503786

  19. Maintenance of self-incompatibility in peripheral populations of a circumboreal woodland subshrub.

    Zhang, Ai-Qin; Xiong, Ying-Ze; Huang, Shuang-Quan

    2014-01-01

    Compared with self-incompatible (SI) species, species that shift to self-compatibility (SC) are more likely to colonize a new habitat. Self-incompatibility and fruit-set failure have been widely reported in European populations of Linnaea borealis (twinflower), whereas at the eastern margin of its North American distribution it showed potential SC. We investigated the breeding system of L. borealis in northwestern China, the eastern margin of the species' distribution in Eurasia. Pollinators, breeding system and pollen limitation were examined in a nature reserve with thousands of L. borealis individuals. To investigate whether fruit set was limited by mating opportunity, we compared fruit set in high-, medium- and low-density patches of L. borealis. To examine whether clonal reproduction resulted in higher fruit-set failure, we compared fruit set among different sizes of clonal ramets. Flies contributed most pollinator visits in the studied population. It was strictly SI and natural fruit set depended on insect visits. Patch density comparisons showed that L. borealis was not pollen limited in low-density patches that had significantly fewer flowers. However, it produced significantly fewer fruits per flower when clonal ramet size increased, suggesting that the high failure of fruit set in larger clones with more flowers may be caused by geitonogamy. Generalist pollinators and clonal reproduction may help L. borealis to colonize in marginal areas without the transition of the breeding system from SI to SC, but experiencing fruit-set failure resulting from geitonogamy within clones. PMID:25336338

  20. Evidence for the long-term maintenance of a rare self-incompatibility system in Oleaceae.

    Vernet, Philippe; Lepercq, Pierre; Billiard, Sylvain; Bourceaux, Angélique; Lepart, Jacques; Dommée, Bertrand; Saumitou-Laprade, Pierre

    2016-06-01

    A rare homomorphic diallelic self-incompatibility (DSI) system discovered in Phillyrea angustifolia (family Oleaceae, subtribe Oleinae) can promote the transition from hermaphroditism to androdioecy. If widespread and stable in Oleaceae, DSI may explain the exceptionally high rate of androdioecious species reported in this plant family. Here, we set out to determine whether DSI occurs in another Oleaceae lineage. We tested for DSI in subtribe Fraxininae, a lineage that diverged from subtribe Oleinae c. 40 million yr ago. We explored the compatibility relationships in Fraxinus ornus using 81 hermaphrodites and 25 males from one natural stand and two naturalized populations using intra- and interspecific stigma tests performed on F. ornus and P. angustifolia testers. We uncovered a DSI system with hermaphrodites belonging to one of two self-incompatibility (SI) groups and males compatible with both groups, making for a truly androdioecious reproductive system. The two human-founded populations contained only one of the two SI groups. Our results provide evidence for the evolutionary persistence of DSI. We discuss how its stability over time may have affected transitions to other sexual systems, such as dioecy. PMID:26833140

  1. Differentiation and functional connection of vascular elements in compatible and incompatible pear/quince internode micrografts.

    Espen, Luca; Cocucci, Maurizio; Sacchi, Gian Attilio

    2005-11-01

    Micrografts of internodes excised from in vitro grown pear plants (Pyrus communis L. cv. 'Bosc' (B) and cv. 'Butirra Hardy' (BH)) and quince (Cydonia oblonga Mill. East Malling clone C (EMC)), were cultured aseptically to test the effectiveness of their functional vascular reconnection in relation to incompatibility-compatibility relationships that these genotypes exhibit in the field. The incompatible heterograft (B/EMC) showed a marked delay in internode cohesion compared with the autografts (both B/B and BH/BH) and the compatible heterograft (BH/EMC). Even when fused, the translocation of [14C]-sorbitol from upper to lower internode was lower in B/EMC micrografts than in the other combinations. Epifluorescence studies performed with carboxyfluorescin, a specific phloem probe, indicated that the limited translocation was caused by a delay in the establishment of functional phloem continuity between the two internodes. In the B/EMC combination, new differentiated tracheary elements (TE) in the parenchyma tissue at the graft interface between the two internodes were not detected until 30 days after grafting, whereas in the BH/EMC heterograft and both autografts, new xylem connections appeared to cross the interface 20 days after grafting. Immunohistochemical detection (terminal nick-end labeling assay) of the number of cells undergoing nuclear DNA fragmentation at the graft interface confirmed that the limited and delayed TE differentiation in B/EMC heterografts was associated with a decrease in the activity of programmed cell death processes involved in the differentiation of TE. PMID:16105809

  2. Successful treatment of severe rhesus D-incompatible pregnancy with repeated double-filtration plasmapheresis.

    Kamei, Koichi; Yamaguchi, Koushi; Sato, Mai; Ogura, Masao; Ito, Shuichi; Okada, Tomomi; Wada, Seiji; Sago, Haruhiko

    2015-10-01

    Fetal anemia is caused by Rhesus (RhD) sensitization as a result of RhD incompatibility during pregnancy. The severe form of this disease can cause hydrops fetalis leading to intrauterine death. We experienced a highly sensitized 39-year-old woman with B Rh-negative blood. She had a history of three induced abortions and experienced perinatal death associated with hydrops fetalis. During the pregnancy prior to her most recent one, she was treated with double-filtration plasmapheresis (DFPP), high dose γ-globulin and intrauterine fetal blood transfusion (IUT). For her most recent pregnancy, we performed only weekly or fortnightly DFPP from 13 weeks until delivery. Anti-D antibody titer was maintained between 32 and 256 without any signs of fetal anemia. IUT was not required at any stage of the pregnancy. No adverse events were observed. She successfully delivered a healthy male infant weighing 2,289 g by Cesarean section at 35 weeks. Repeated DFPP may be an effective and safe strategy to reduce antibody titers in highly sensitized women with RhD-incompatible pregnancy, avoiding the need for IUT. PMID:25413689

  3. On the genetic architecture of cytoplasmic incompatibility: inference from phenotypic data.

    Nor, Igor; Engelstädter, Jan; Duron, Olivier; Reuter, Max; Sagot, Marie-France; Charlat, Sylvain

    2013-07-01

    Numerous insects carry intracellular bacteria that manipulate the insects' reproduction and thus facilitate their own spread. Cytoplasmic incompatibility (CI) is a common form of such manipulation, where a (currently uncharacterized) bacterial modification of male sperm induces the early death of embryos unless the fertilized eggs carry the same bacteria, inherited from the mother. The death of uninfected embryos provides an indirect selective advantage to infected ones, thus enabling the spread of the bacteria. Here we use and expand recently developed algorithms to infer the genetic architecture underlying the complex incompatibility data from the mosquito Culex pipiens. We show that CI requires more genetic determinants than previously believed and that quantitative variation in gene products potentially contributes to the observed CI patterns. In line with population genetic theory of CI, our analysis suggests that toxin factors (those inducing embryo death) are present in fewer copies in the bacterial genomes than antitoxin factors (those ensuring that infected embryos survive). In combination with comparative genomics, our approach will provide helpful guidance to identify the genetic basis of CI and more generally of other toxin/antitoxin systems that can be conceptualized under the same framework. PMID:23778233

  4. Smallness of the number of incompatibility loci can facilitate parapatric speciation.

    Yamaguchi, Ryo; Iwasa, Yoh

    2016-09-21

    We studied the time to speciation by geographic isolation for a species living on two islands connected by infrequent migration. Assumptions were that incompatibility was controlled by a finite number of quantitative loci, and individuals differing in loci of more than some threshold fraction do not mix genetically with each other. We also assumed sexual haploid species, each population being nearly monomorphic, and free recombination between loci for within-population processes. The genetic distance (defined as the fraction of loci differing between populations) followed stochastic processes, which were analyzed by means of stochastic differential equations, diffusion equations, and individual-based simulations. The distance increases by the accumulation of novel mutations but decreases by migration and hybridization. It may converge to a quasi-equilibrium around which it fluctuates thereafter. If the threshold fraction of speciation is controlled, the smallness of the number of incompatibility loci enhanced the magnitude of fluctuation around the quasi-equilibrium and shortened the time to speciation considerably. Novel species were created by mutation accumulation and repeated infrequent migration, and the rate of species creation was the fastest for an intermediate rate of migration. A smaller number of loci increased the optimal migration rate and the species creation rate. PMID:26582724

  5. Wolbachia age-sex-specific density in Aedes albopictus: a host evolutionary response to cytoplasmic incompatibility?

    Pablo Tortosa

    Full Text Available BACKGROUND: Wolbachia bacteria have invaded many arthropod species by inducing Cytoplasmic Incompatibility (CI. These symbionts represent fascinating objects of study for evolutionary biologists, but also powerful potential biocontrol agents. Here, we assess the density dynamics of Wolbachia infections in males and females of the mosquito Aedes albopitcus, an important vector of human pathogens, and interpret the results within an evolutionary framework. METHODOLOGY/PRINCIPAL FINDINGS: Wolbachia densities were measured in natural populations and in age controlled mosquitoes using quantitative PCR. We show that the density dynamics of the wAlbA Wolbachia strain infecting Aedes albopictus drastically differ between males and females, with a very rapid decay of infection in males only. CONCLUSIONS/SIGNIFICANCE: Theory predicts that Wolbachia and its hosts should cooperate to improve the transmission of infection to offspring, because only infected eggs are protected from the effects of CI. However, incompatible matings effectively lower the fertility of infected males, so that selection acting on the host genome should tend to reduce the expression of CI in males, for example, by reducing infection density in males before sexual maturation. The rapid decay of one Wolbachia infection in Aedes albopictus males, but not in females, is consistent with this prediction. We suggest that the commonly observed reduction in CI intensity with male age reflects a similar evolutionary process. Our results also highlight the importance of monitoring infection density dynamics in both males and females to assess the efficiency of Wolbachia-based control strategies.

  6. Foco epidémico de sirenomelia en Cali, Colombia. Informe de 4 casos en el Hospital Universitario del Valle en 54 días

    Armicson Felipe Solano

    2006-09-01

    Full Text Available La sirenomelia es una malformación congénita rara que se caracteriza por fusión de los miembros inferiores, asociada con malformaciones viscerales que pueden ser incompatibles con la vida. La etiología se desconoce. Se informa la prevalencia entre 1 y 2 en 100,000 nacimientos. Se presentan 4 casos de sirenomelia en niños recién nacidos en el Hospital Universitario del Valle de Cali, Colombia entre el 9 de diciembre de 2004 y el 2 de febrero de 2005, lo que correspondería a una prevalencia de 3.5 casos por 1000 nacimientos para ese período, que serían 175 veces más de lo informado en la literatura.

  7. Frequency of ABO and Rh (D Blood Groups and Hemoglobin Threshold among Pregnant Women in Family Guidance Association, Mekelle Model Clinic, North Ethiopia.

    Megbaru Alemu

    2014-12-01

    Full Text Available This study was aimed to determine the frequency of ABO and Rh blood group patterns and hemoglobin threshold among pregnant women screened at Family Guidance Association of Ethiopia, North Area Mekelle model clinic. Checklists were prepared to collect data from laboratory registration books of five years to undergo this retrospective study. Those registration books were reviewed for ABO blood group, Rh profiles and hemoglobin threshold of the pregnant women screened during the specified period. A total of 5987 pregnant women have been found to be screened and the predominant phenotype in this study was O (41.5% followed by phenotype A (28%, Phenotype B (25% and phenotype AB (5.5%. Regarding Rh (D system, 91.2% (5458/5987 women were positive for rhesus D antigen while the remaining 8.8% (529/5987 women were negative for the antigen. Blood grouping studies should be done in every region of the nation so that national transfusion policies can be drafted and supplying blood to the needy patients during emergency will be easier.

  8. Novel association of ABO histo-blood group antigen with soluble ICAM-1: results of a genome-wide association study of 6,578 women.

    Guillaume Paré

    2008-07-01

    Full Text Available While circulating levels of soluble Intercellular Adhesion Molecule 1 (sICAM-1 have been associated with diverse conditions including myocardial infarction, stroke, malaria, and diabetes, comprehensive analysis of the common genetic determinants of sICAM-1 is not available. In a genome-wide association study conducted among 6,578 participants in the Women's Genome Health Study, we find that three SNPs at the ICAM1 (19p13.2 locus (rs1799969, rs5498 and rs281437 are non-redundantly associated with plasma sICAM-1 concentrations at a genome-wide significance level (P<5x10(-8, thus extending prior results from linkage and candidate gene studies. We also find that a single SNP (rs507666, P = 5.1x10(-29 at the ABO (9q34.2 locus is highly correlated with sICAM-1 concentrations. The novel association at the ABO locus provides evidence for a previously unknown regulatory role of histo-blood group antigens in inflammatory adhesion processes.

  9. Preliminary joint neutron time-of-flight and X-ray crystallographic study of human ABO(H) blood group A glycosyltransferase

    Collection to 2.5 Å resolution of neutron diffraction data from crystals of the human ABO(H) blood group glycosyltransferase GTA, is presented with the preliminary joint refinement with the corresponding X-ray diffraction data. The biosyntheses of oligosaccharides and glycoconjugates are conducted by glycosyltransferases. These extraordinarily diverse and widespread enzymes catalyze the formation of glycosidic bonds through the transfer of a monosaccharide from a donor molecule to an acceptor molecule, with the stereochemistry about the anomeric carbon being either inverted or retained. Human ABO(H) blood group A α-1,3-N-acetylgalactosaminyltransferase (GTA) generates the corresponding antigen by the transfer of N-acetylgalactosamine from UDP-GalNAc to the blood group H antigen. To understand better how specific active-site-residue protons and hydrogen-bonding patterns affect substrate recognition and catalysis, neutron diffraction studies were initiated at the Protein Crystallography Station (PCS) at Los Alamos Neutron Science Center (LANSCE). A large single crystal was subjected to H/D exchange prior to data collection and time-of-flight neutron diffraction data were collected to 2.5 Å resolution at the PCS to ∼85% overall completeness, with complementary X-ray diffraction data collected from a crystal from the same drop and extending to 1.85 Å resolution. Here, the first successful neutron data collection from a glycosyltransferase is reported

  10. Centauros Mensajeros - Casos de estudio

    Bolívar Ramírez, Jorge Enrique

    2012-01-01

    El estudio del caso de la empresa Centaurus Mensajeros es presentado en un documento estructurado en tres partes, donde primero se expone la historia y las características del proyecto; luego se presenta su entorno empresarial; y finalmente, se muestra un ejemplo de emprendimiento a través de la historia de vida del empresario y fundador de esta organización, así como su gestión, cualidades como empresario y lucha por mantener la empresa de Centaurus Mensajeros como una de las más reconocidas...

  11. La tesis del caso especial

    Alexy, Robert

    1999-01-01

    Not available

    A partir de la comprensión de la tesis del caso especial como un intento por dar cuenta de la doble vertiente de la argumentación jurídica (su carácter autoritativo, institucional o real, por un lado, y su dimensión libre, discursiva o ideal, por otro) Alexy se propone defender esta tesis frente a las críticas tomadas en consideración o realizadas por Jürgen Habermas en su libro Facticidad y validez. En primer lugar, Alexy defiende su tesis ...

  12. Incompatibility behavior of a symbiotic plasmid pMH7653Rb in Mesorhizobium huakuii 7653R

    2010-01-01

    Mesorhizobium huakuii strain 7653R harbored two indigenous plasmids named pMH7653Ra and pMH7653Rb.The larger plasmid pMH7653Rb (symbiotic plasmid) was transferred to M.huakuii HN308SR harboring three plasmids: pMHHN308a,pMHHN308b and pMHHN308c,and HN3015SR harboring three plasmids: pMHHN3015a,pMHHN3015b and pMHHN3015c by tri-parent mating.Two stable indigenous plasmids,pMHHN308b and pMHHN308c of HN308SR,were co-eliminated due to the introduction of pMH7653Rb,and the transconjugant was named HN308SRN14.The results implied that pMH7653Rb and pMHHN308b,pMHHN308c were incompatible and might have been ascribed to the same incompatible group.The plasmid profiles of transconjugant HN3015SRN14 showed that the second largest plasmid pMHHN3015b of HN3015SR was cured due to the introduction of pMH7653Rb.The results also implied that pMH7653Rb and pMHHN3015b were incompatible.Results from plant nodulation tests showed that pMH7653Rb could only maintain the nodulation ability in transconjugant HN308SRN14 and its nodule number was more than that of wild strain HN308SR,but could not replace the nitrogen fixation effect of pMHHN308b and pMHHN308c.The plasmid cured mutant HN308SRN14D harboring only pMHHN308a formed null nodules that demonstrated pMHHN308a was relevant to nodulation ability.HN3015SRN14 harboring pMH7653Rb,pMHHN3015a and pMHHN3015c formed null nodules while HN3015SRN14D containing pMHHN3015a and pMHHN3015c lost the nodulation ability.The plasmid replication repC-like gene sequences were detected by a polymerase chain reaction from 7653R,HN308,HN3015,HN308SRN14 and HN3015SRN14.The repC gene sequence similarities of the strains tested attained 99%.

  13. PollenCALC: Software for estimation of pollen compatibility of self-incompatible allo- and autotetraploid species

    Aguirre, Andrea A; Wollenweber, Bernd; Frei, Ursula K;

    2012-01-01

    Background Self-incompatibility (SI) is a biological mechanism to avoid inbreeding in allogamous plants. In grasses, this mechanism is controlled by a two-locus system (S-Z). Calculation of male and female gamete frequencies is complex for tetraploid species. We are not aware of any software...... available for predicting pollen haplotype frequencies and pollen compatibility in tetraploid species. Results PollenCALC is a software tool written in C++ programming language that can predict pollen compatibility percentages for polyploid species with a two-locus (S, Z) self-incompatibility system. The...

  14. lon Incompatibility Associated with Mutations Causing SOS Induction: Null uvrD Alleles Induce an SOS Response in Escherichia coli

    SaiSree, L.; Reddy, Manjula; Gowrishankar, J

    2000-01-01

    The uvrD gene in Escherichia coli encodes a 720-amino-acid 3′-5′ DNA helicase which, although nonessential for viability, is required for methyl-directed mismatch repair and nucleotide excision repair and furthermore is believed to participate in recombination and DNA replication. We have shown in this study that null mutations in uvrD are incompatible with lon, the incompatibility being a consequence of the chronic induction of SOS in uvrD strains and the resultant accumulation of the cell s...

  15. Trouble with the Lorentz law of force: Incompatibility with special relativity and momentum conservation

    Mansuripur, Masud

    2012-01-01

    The Lorentz law of force is the fifth pillar of classical electrodynamics, the other four being Maxwell's macroscopic equations. The Lorentz law is the universal expression of the force exerted by electromagnetic fields on a volume containing a distribution of electrical charges and currents. If electric and magnetic dipoles also happen to be present in a material medium, they are traditionally treated by expressing the corresponding polarization and magnetization distributions in terms of bound-charge and bound-current densities, which are subsequently added to free-charge and free-current densities, respectively. In this way, Maxwell's macroscopic equations are reduced to his microscopic equations, and the Lorentz law is expected to provide a precise expression of the electromagnetic force density on material bodies at all points in space and time. This paper presents incontrovertible theoretical evidence of the incompatibility of the Lorentz law with the fundamental tenets of special relativity. We argue t...

  16. Protecting a quantum state from environmental noise by an incompatible finite-time measurement

    Brasil, Carlos Alexandre; Napolitano, Reginaldo de Jesus

    2011-01-01

    We show that measurements of finite duration performed on an open two-state system can protect the initial state from a phase-noisy environment, provided the measured observable does not commute with the perturbing interaction. When the measured observable commutes with the environmental interaction, the finite-duration measurement accelerates the rate of decoherence induced by the phase noise. For the description of the measurement of an observable that is incompatible with the interaction between system and environment, we have found an approximate analytical expression, valid at zero temperature and weak coupling with the measuring device. We have tested the validity of the analytical predictions against an exact numerical approach, based on the superoperator-splitting method, that confirms the protection of the initial state of the system. When the coupling between the system and the measuring apparatus increases beyond the range of validity of the analytical approximation, the initial state is still prot...

  17. The Beta Problem: The Incompatibility of X-ray and Sunyaev-Zeldovich Model Fitting

    Burns, Jack O.; Hallman, E.; Motl, P.; Norman, M.

    2006-12-01

    We describe an analysis of a large sample of numerically simulated clusters which demonstrates the effects of using X-ray fitted beta-model parameters with Sunyaev-Zeldovich effect (SZE) data. There is a fundamental incompatibility between beta-model fits to X-ray surface brightness profiles and those done with SZE profiles. Since observational SZE radial profiles are in short supply, the X-ray parameters are often used in SZE analysis. We show that this leads to biased estimates of the integrated Compton y-parameter inside r500 and the value of the Hubble constant calculated from clusters. We suggest a simple scaling of the X-ray beta-model parameters which brings these calculated quantities into close agreement with the true values.

  18. Female employment and fertility in Peninsular Malaysia: the maternal role incompatibility hypothesis reconsidered.

    Mason, K O; Palan, V T

    1981-11-01

    Multivariate analysis of the 1974 Malaysian Fertility and Family Survey tests the hypothesis that an inverse relationship between women's work and fertility occurs only when there are serious conflicts between working and caring for children. The results are only partly consistent with the hypothesis and suggest that normative conflicts between working and mothering affect the employment-fertility relationship in Malaysia more than spacio-temporal conflicts do. The lack of consistent evidence for the hypothesis, as well as some conceptual problems, lead us to propose an alternative framework for understanding variation in the employment-fertility relationship, both in Malaysia and elsewhere. This framework incorporates ideas from the role incompatibility hypothesis but views the employment-fertility relationship as dependent not just on role conflicts but more generally on the structure of the household's socioeconomic opportunities. PMID:7308536

  19. LEFT LOBE LIVER TRANSPLANTATION FROM AB0-INCOMPATIBLE LIVING DONOR WITH SITUS INVERSUS

    S. V. Gautier

    2014-12-01

    Full Text Available Situs inversus is a rare congenital abnormality that affects approximately 0.005% of all live births. Traditionally, this condition is considered as a contraindication for liver donation, primarily due to the peculiarities of the vascular anatomy and the diffi culties in graft placement in the abdominal cavity. Review of the world literature testifi es to fi ve cases of use of the whole liver from deceased donor with situs inversus in adult recipients, and to just one case of inverted right lobe transplantation from living donor to 53-year-old man. Thus, transplantation of an inverted left liver lobe from a living related donor in pediatric patients was performed for the fi rst time. The article presents a successful experience of liver transplantation in child with tyrosinemia type 1 from AB0-incompatible living donor with situs inversus.

  20. Flowering time and incompatibility groups: Cultivar combination in commercial sweet cherry (Prunus avium L. orchards

    Radičević Sanja

    2011-01-01

    Full Text Available The paper presents results of eight-year study (1999-2006 of flowering phenophase in 21 introduced sweet cherry cultivars grown under the agro-environmental conditions of West Serbia. Flowering time, as well as progress and abundance of flowering were studied, and classification of the studied cultivars according to flowering time was derived. On the basis of mean several-year overlap in phenophase of full flowering and on the grounds of so far known data on classification of these cultivars among incompatibility groups, we have offered a recommendation for their cultivation in orchards whereby the most effective pollination and fertilization can be ensured as well as good fruit-set and satisfactory fruit yields.

  1. Using Web Services Standards for Dealing with Complexities of Multiple Incompatible Applications

    Ghartey Nana Gyankumah

    2012-10-01

    Full Text Available Organizations’ dependence on custom enterprise software and web applications from independent software developers and software companies create a lot of problems such as integration, interoperability, security, and system maintenance. This paper seeks to provide a better approach by using Web services standards in dealing with the complexity of multiple incompatible applications that were written in different programming languages on multiple computers and also making it possible for organizations to add a new layer of abstraction that is open, standards-based, and easy to integrate with any new or existing system. The combination of Service Oriented Architecture and Web services will be used to provide a rapid integration solution that will quickly and easily align Information Technology investments and corporate strategies by focusing on shared data and reusable services rather than proprietary integration products.

  2. Proteomics changes during the incompatible interaction between cowpea and Colletotrichum gloeosporioides (Penz.) Penz and Sacc.

    Moura, Hudson Fernando N; Vasconcelos, Ilka M; Souza, Carlos Eduardo A; Silva, Fredy D A; Moreno, Frederico B M B; Lobo, Marina D P; Monteiro-Moreira, Ana C O; Moura, Arlindo A; Costa, José H; Oliveira, José Tadeu A

    2014-03-01

    Anthracnose represents an important disease of cowpea [Vigna unguiculata L. (Walp.)] caused by the hemibiothrophic fungus Colletotrichum gloeosporioides that drastically reduces cowpea field production. In this study we investigated some biochemical aspects underlying the incompatible interaction between a resistant cowpea genotype and C. gloeosporioides using a proteomic approach. Analyses of two-dimensional gel electrophoresis patterns and protein identification indicate C. gloeosporioides infection-dependent cowpea leaf proteome changes associated with metabolism, photosynthesis, response to stress, oxidative burst and scavenging, defense signaling, and pathogenesis-related proteins. Moreover the C. gloeosporioides responsive proteins interaction network in cowpea revealed the interconnected modulation of key cellular processes involving particularly antioxidants proteins, photosynthetic apparatus forming proteins and proteins of the energetic metabolism that interact with each other suggesting that their expression changes are also important for resistance of cowpea to C. gloeosporioides. PMID:24467908

  3. The incompatibility between local hidden variable theories and the fundamental conservation laws

    C S Unnikrishnan

    2005-09-01

    I discuss in detail the result that the Bell's inequalities derived in the context of local hidden variable theories for discrete quantized observables can be satisfied only if a fundamental conservation law is violated on the average. This result shows that such theories are physically nonviable, and makes the demarcating criteria of the Bell's inequalities redundant. I show that a unique correlation function can be derived from the validity of the conservation law alone and this coincides with the quantum mechanical correlation function. Thus, any theory with a different correlation function, like any local hidden variable theory, is incompatible with the fundamental conservation laws and space-time symmetries. The results are discussed in the context of two-particle singlet and triplet states, GHZ states, and two-particle double slit interferometry. Some observations on quantum entropy, entanglement, and nonlocality are also discussed.

  4. Wolbachia-induced cytoplasmic incompatibility as a means for insect pest population control

    Zabalou, Sofia; Riegler, Markus; Theodorakopoulou, Marianna; Stauffer, Christian; Savakis, Charalambos; Bourtzis, Kostas

    2004-01-01

    Biological control is the purposeful introduction of parasites, predators, and pathogens to reduce or suppress pest populations. Wolbachia are inherited bacteria of arthropods that have recently attracted attention for their potential as new biocontrol agents. Wolbachia manipulate host reproduction by using several strategies, one of which is cytoplasmic incompatibility (CI) [Stouthamer, R., Breeuwer, J. A. J. & Hurst, G. D. D. (1999) Annu. Rev. Microbiol. 53, 71–102]. We established Wolbachia-infected lines of the medfly Ceratitis capitata using the infected cherry fruit fly Rhagoletis cerasi as donor. Wolbachia induced complete CI in the novel host. Laboratory cage populations were completely suppressed by single releases of infected males, suggesting that Wolbachia-induced CI could be used as a novel environmentally friendly tool for the control of medfly populations. The results also encourage the introduction of Wolbachia into pest and vector species of economic and hygenic relevance to suppress or modify natural populations. PMID:15469918

  5. Un caso de psicosis infantil

    Andrea Obando Cuellar

    2012-01-01

    Full Text Available Se expondrá el caso de Samuel un niño de 8 años de edad hospitalizado aparentemente por un cuadrode ansiedad, que en su evolución, y acorde a la evaluación clínica, expone claramente la sintomatología de una psicosis infantil. El proceso de evaluación indica cuadro psicótico infantil, presencia de fobias arcaicas, terrores sin nombre y desestructuración de la organización lingüística. Su sintomatología refiere manifestaciones somáticas, una sensación constante de amenazas externas a su juicio de realidad; y sensaciones de despersonalización y desbordamientos de su pensamiento. El mundo de las ideas sobrepasa al mundo de los afectos. Se presenta un infante matizado por fantasías de aniquilamiento y desafíos constantes de la aceptación de la realidad. Sobre el caso de Samuel, cabe mencionar que durante el proceso de hospitalización y recursos, los padres no concretarola asistencia terapéutica, ni la posibilidad de brindar un espacio analítico para la comprensión de la lógica y significado del evento traumático y la fantasía desorganizadora de la pérdida paterna. Nos preguntamos cuál era el significante de Samuel.

  6. Importancia de la autopsia fetal en casos de abortos espontáneos.

    Tanya Kitova

    2014-08-01

    Full Text Available Objetivos: Subrayar la necesidad y la importancia de las investigaciones fetopatológicas en casos de abortos espontáneos.Material y métodología: En Bulgaria y Vietnam se ha llevado a cabo la investigación fetopatológica de dos fetos obtenidos en casos de abortos espontáneos entre la decimonona y la vigésimoprimera semana de gestación. Se ha aplicado la autopsia clásica de fetos, conservados a base de disolución de formaldehído en agua en una proporción de 40% en peso, acompañada por examen externo, análisis biométrico y disección in situ. Se ha obtenido material fotográfico de los hallazgos patológicos y material para el examen microscópico.Resultados: En el primer feto la malformación se ha diagnosticadf como un caso de cranio-rachischisis totalis, asociada con anomalías faciales, anomalías de las extremidades, anomalías del aparato excretor, el aparato reprodutor y el sistema digestivo. En el segundo feto se ha diagnosticado anencefalia (acrania asociada con anomalías faciales, anomalías de las extremidades y de los órganos internos.Discusión: En ambos casos se han descubierto defectos del tubo neural (DTN relacionados con las grandes anomalías, incompatibles con la vida, que tienen una frecuencia de entre 0.5 a 6 casos en cada 1000 partos. La presente investigación comprueba que el aborto espontáneo y las causas que lo provocan no han de ser menospreciados. El estudio pone énfasis en la importancia de la autopsia en la medicina fetal y su inapreciable valor para la perspectiva del próximo embarazo

  7. Non-Random Mating, Parent-of-Origin, and Maternal-Fetal Incompatibility Effects in Schizophrenia

    Kim, Yunjung; Ripke, Stephan; Kirov, George; Sklar, Pamela; Purcell, Shaun; Owen, Michael; O’Donovan, Michael C.; Sullivan, Patrick F.

    2014-01-01

    Although the association of common genetic variation in the extended MHC region with schizophrenia is the most significant yet discovered, the MHC region is one of the more complex regions of the human genome, with unusually high gene density and long-range linkage disequilibrium. The statistical test on which the MHC association is based is a relatively simple, additive model which uses logistic regression of SNP genotypes to predict case-control status. However, it is plausible that more complex models underlie this association. Using a well-characterized sample of trios, we evaluated more complex models by looking for evidence for: (a) non-random mating for HLA alleles, schizophrenia risk profiles, and ancestry; (b) parent-of-origin effects for HLA alleles; and (c) maternal-fetal genotype incompatibility in the HLA. We found no evidence for non-random mating in the parents of individuals with schizophrenia in terms of MHC genotypes or schizophrenia risk profile scores. However, there was evidence of non-random mating that appeared mostly to be driven by ancestry. We did not detect over-transmission of HLA alleles to affected offspring via the general TDT test (without regard to parent of origin) or preferential transmission via paternal or maternal inheritance. We evaluated the hypothesis that maternal-fetal HLA incompatibility may increase risk for schizophrenia using eight classical HLA loci. The most significant alleles were in HLA-B, HLA-C, HLA-DQB1, and HLA-DRB1 but none was significant after accounting for multiple comparisons. We did not find evidence to support more complex models of gene action, but statistical power may have been limiting. PMID:23177929

  8. Convergent evolution at the gametophytic self-incompatibility system in Malus and Prunus.

    Aguiar, Bruno; Vieira, Jorge; Cunha, Ana E; Fonseca, Nuno A; Iezzoni, Amy; van Nocker, Steve; Vieira, Cristina P

    2015-01-01

    S-RNase-based gametophytic self-incompatibility (GSI) has evolved once before the split of the Asteridae and Rosidae. This conclusion is based on the phylogenetic history of the S-RNase that determines pistil specificity. In Rosaceae, molecular characterizations of Prunus species, and species from the tribe Pyreae (i.e., Malus, Pyrus, Sorbus) revealed different numbers of genes determining S-pollen specificity. In Prunus only one pistil and pollen gene determine GSI, while in Pyreae there is one pistil but multiple pollen genes, implying different specificity recognition mechanisms. It is thus conceivable that within Rosaceae the genes involved in GSI in the two lineages are not orthologous but possibly paralogous. To address this hypothesis we characterised the S-RNase lineage and S-pollen lineage genes present in the genomes of five Rosaceae species from three genera: M. × domestica (apple, self-incompatible (SI); tribe Pyreae), P. persica (peach, self-compatible (SC); Amygdaleae), P. mume (mei, SI; Amygdaleae), Fragaria vesca (strawberry, SC; Potentilleae), and F. nipponica (mori-ichigo, SI; Potentilleae). Phylogenetic analyses revealed that the Malus and Prunus S-RNase and S-pollen genes belong to distinct gene lineages, and that only Prunus S-RNase and SFB-lineage genes are present in Fragaria. Thus, S-RNase based GSI system of Malus evolved independently from the ancestral system of Rosaceae. Using expression patterns based on RNA-seq data, the ancestral S-RNase lineage gene is inferred to be expressed in pistils only, while the ancestral S-pollen lineage gene is inferred to be expressed in tissues other than pollen. PMID:25993016

  9. Integration of irradiation with cytoplasmic incompatibility to facilitate a lymphatic filariasis vector elimination approach

    Dobson Stephen L

    2009-08-01

    Full Text Available Abstract Background Mass drug administration (MDA is the emphasis of an ongoing global lymphatic filariasis (LF elimination program by the World Health Organization, in which the entire 'at risk' human population is treated annually with anti-filarial drugs. However, there is evidence that the MDA strategy may not be equally appropriate in all areas of LF transmission, leading to calls for the augmentation of MDA with anti-vector interventions. One potential augmentative intervention is the elimination of vectors via repeated inundative releases of male mosquitoes made cytoplasmically incompatible via an infection with Wolbachia bacteria. However, with a reduction in the vector population size, there is the risk that an accidental female release would permit the establishment of the incompatible Wolbachia infection type, resulting in population replacement instead of population elimination. To avoid the release of fertile females, we propose the exposure of release individuals to low doses of radiation to sterilize any accidentally released females, reducing the risk of population replacement. Results Aedes polynesiensis pupae of differing ages were irradiated to determine a radiation dose that results in sterility but that does not affect the survival and competitiveness of males. Laboratory assays demonstrate that males irradiated at a female sterilizing dosage of 40 Gy are equally competitive with un-irradiated males. No effect of irradiation on the ability of Wolbachia to affect egg hatch was observed. Conclusion An irradiation dose of 40 Gy is sufficient to cause female sterility, but has no observed negative effect on male fitness. The results support further development of this approach as a preventative measure against accidental population replacement.

  10. Population genetics of self-incompatibility in a clade of relict cliff-dwelling plant species

    Silva, Jose L.; Brennan, Adrian C.; Mejías, José A.

    2016-01-01

    The mating systems of species in small or fragmented populations impact upon their persistence. Small self-incompatible (SI) populations risk losing S allele diversity, responsible for the SI response, by drift thereby limiting mate availability and leading to population decline or SI system breakdown. But populations of relict and/or endemic species have resisted these demographic conditions over long periods suggesting their mating systems have adapted. To address a lack of empirical data on this topic, we studied the SI systems of three relict cliff-dwelling species of Sonchus section Pustulati (Asteraceae): S. masguindalii, S. fragilis and S. pustulatus in the western Mediterranean region. We performed controlled pollinations within and between individuals to measure index of SI (ISI) expression and identify S alleles in multiple population samples. Sonchus masguindalii and S. pustulatus showed strong SI (ISI = 0.6–1.0) compared to S. fragilis (ISI = 0.1–0.7). Just five S alleles were estimated for Spanish S. pustulatus and a moderate 11-15 S alleles for Moroccan S. pustulatus and S. fragilis, respectively. The fact that autonomous fruit set was generally improved by active self-pollination in self-compatible S. fragilis suggests that individuals with weak SI can show a wide range of outcrossing levels dependent on the degree of self or outcross pollen that pollinators bear. We conclude that frequent S allele dominance interactions that mask the incompatibility interactions of recessive S alleles leading to higher mate availability and partial breakdown of SI leading to mixed mating, both contribute to reproductive resilience in this group. PMID:27154621

  11. Characteristics of the ion beam exposure for overcoming the cross incompatibility

    In wild species, there are valuable genes controlling resistance to disease, insect and environmental stress. The introduction of such genes from wild species to cultivar is extremely useful in plant breeding. However, it is very difficult or impossible to obtain an interspecific hybrid in a wide cross, because of the strong cross-incompatibility. We have been searching for an effective procedure of interspecific hybridization in Nicotiana. Because N. gossei Domin (wild species) has been reported to be resistant to more disease and insects than other Nicotiana species, many attempts to hybridize it with N. tabacum L. (cultivar) have been made. In N. gossei x N. tabacum, it is easy to get hybrid seeds with conventional cross, but hybrid plants can not survive, On the other hand, in N. tabacum x N. gossei, few seeds with viability can be obtained. In this species combination, we had obtained the viable hybrid plant by means of the cross with the gamma-rays-irradiated pollen at the rate of 3.7 x 10-5. Ion beams have higher LET (Linear Energy Transfer), in comparison with that of gamma-rays, and can be controlled the range in the target material. Accordingly, a lot of energy can be deposited on the focused point of material exposed to ion beams. The cross (N. gossei x N. tabacum) with pollen exposed to ion beams, e. g., he, C and Ne ions, produced the viable hybrid plants at the rate 1 x 10-2, which was much higher than that of gamma-rays. Morphological characters in some of hybrid plants were intermediate between parents and uniform, suggesting that they had no mutation induced by the ion beam exposure. Conclusively, it is said that ion beams are more effective for overcoming the cross incompatibility than radiations with low LET. (author)

  12. Complex Adaptive Systems of Systems (CASOS) engineering environment.

    Detry, Richard Joseph; Linebarger, John Michael; Finley, Patrick D.; Maffitt, S. Louise; Glass, Robert John, Jr.; Beyeler, Walter Eugene; Ames, Arlo Leroy

    2012-02-01

    Complex Adaptive Systems of Systems, or CASoS, are vastly complex physical-socio-technical systems which we must understand to design a secure future for the nation. The Phoenix initiative implements CASoS Engineering principles combining the bottom up Complex Systems and Complex Adaptive Systems view with the top down Systems Engineering and System-of-Systems view. CASoS Engineering theory and practice must be conducted together to develop a discipline that is grounded in reality, extends our understanding of how CASoS behave and allows us to better control the outcomes. The pull of applications (real world problems) is critical to this effort, as is the articulation of a CASoS Engineering Framework that grounds an engineering approach in the theory of complex adaptive systems of systems. Successful application of the CASoS Engineering Framework requires modeling, simulation and analysis (MS and A) capabilities and the cultivation of a CASoS Engineering Community of Practice through knowledge sharing and facilitation. The CASoS Engineering Environment, itself a complex adaptive system of systems, constitutes the two platforms that provide these capabilities.

  13. JEMs and incompatible occupational coding systems: Effect of manual and automatic recoding of job codes on exposure assignment

    Koeman, T.; Offermans, N.S.M.; Christopher-De Vries, Y.; Slottje, P.; Brandt, P.A. van den; Goldbohm, R.A.; Kromhout, H.; Vermeulen, R.

    2013-01-01

    Background: In epidemiological studies, occupational exposure estimates are often assigned through linkage of job histories to job-exposure matrices (JEMs). However, available JEMs may have a coding system incompatible with the coding system used to code the job histories, necessitating a translatio

  14. Dual Perspectives on Theory in Clinical Practice: Practice Makes Perfect: The Incompatibility of Practicing Speech and Meaningful Communication.

    Kamhi, Alan G.

    2000-01-01

    This article uses a case study to suggest that some children view speech-language therapy as a separate situation for learning practicing new sounds and language forms whereas the purpose of talking outside of therapy is meaningful communication. Clinical implications of this potential incompatibility between practicing speech and communicating…

  15. Hybrid incompatibilities in the parasitic wasp genus Nasonia : Negative effects of hemizygosity and the identification of transmission ratio distortion loci

    Koevoets, T.; Niehuis, O.; van de Zande, L.; Beukeboom, L. W.

    2012-01-01

    The occurrence of hybrid incompatibilities forms an important stage during the evolution of reproductive isolation. In early stages of speciation, males and females often respond differently to hybridization. Haldane's rule states that the heterogametic sex suffers more from hybridization than the h

  16. The effect of Wolbachia-induced cytoplasmic incompatibility on host population size in natural and manipulated systems.

    Dobson, Stephen L; Fox, Charles W; Jiggins, Francis M

    2002-03-01

    Obligate, intracellular bacteria of the genus Wolbachia often behave as reproductive parasites by manipulating host reproduction to enhance their vertical transmission. One of these reproductive manipulations, cytoplasmic incompatibility, causes a reduction in egg-hatch rate in crosses between individuals with differing infections. Applied strategies based upon cytoplasmic incompatibility have been proposed for both the suppression and replacement of host populations. As Wolbachia infections occur within a broad range of invertebrates, these strategies are potentially applicable to a variety of medically and economically important insects. Here, we examine the interaction between Wolbachia infection frequency and host population size. We use a model to describe natural invasions of Wolbachia infections, artificial releases of infected hosts and releases of sterile males, as part of a traditional sterile insect technique programme. Model simulations demonstrate the importance of understanding the reproductive rate and intraspecific competition type of the targeted population, showing that releases of sterile or incompatible individuals may cause an undesired increase in the adult number. In addition, the model suggests a novel applied strategy that employs Wolbachia infections to suppress host populations. Releases of Wolbachia-infected hosts can be used to sustain artificially an unstable coexistence of multiple incompatible infections within a host population, allowing the host population size to be reduced, maintained at low levels, or eliminated. PMID:11886634

  17. Wolbachia density and cytoplasmic incompatibility in Aedes albopictus: concerns with using artificial Wolbachia infection as a vector suppression tool.

    Calvitti, Maurizio; Marini, Francesca; Desiderio, Angiola; Puggioli, Arianna; Moretti, Riccardo

    2015-01-01

    The mosquito Aedes albopictusi is a competent vector of harmful human pathogens, including viruses causing dengue and chikungunya. Cytoplasmic incompatibility (CI) induced by endosymbiotic Wolbachia can be used to produce functionally sterile males that can be released in the field as a suppression tool against this mosquito. Because the available sexing methods are not efficient enough to avoid unintentional release of a few transinfected females, we assessed the CI pattern in crosses between wPip Wolbachia-transinfected (ARwP) females and wild-type males of Ae. albopictus in this study. Quantitative polymerase chain reaction was used to monitor the titer of the Wolbachia strains that naturally infect Ae. albopictus, that is, wAlbA and wAlbB, in age-controlled males and females. Data were coupled with incompatibility level detected when the above-mentioned males were crossed with ARwP females. Wolbachia infection titer was also monitored in samples of wild caught males. Incompatibility level was positively correlated only with wAlbA density. Crosses between wild-type males having very low wAlbA density (incompatible insect technique. Various alternative strategies have been discussed to prevent this risk and to exploit Wolbachia as a tool to control Ae. albopictus. PMID:25812130

  18. Bonding at Compatible and Incompatible Amorphous Interfaces of Polystyrene and Poly(Methyl Methacrylate) Below the Glass Transition Temperature

    Boiko, Yuri M.; Lyngaae-Jørgensen, Jørgen

    2004-01-01

    in regard to those at compatible PS-PS and PMMA-PMMA interfaces. The values of strength for the incompatible PS-PMMA and compatible PMMA-PMMA interfaces were found to be close, both being smaller by a factor of 2 to 3 than the values of or for the PS-PS interface developed after healing at the same...

  19. Wine intake, ABO phenotype, and risk of ischemic heart disease and all-cause mortality: the Copenhagen Male Study-a 16-year follow-up

    Suadicani, P.; Hein, H.O.; Gyntelberg, F.

    2008-01-01

    were ABO phenotypes, alcohol intake (wine, beer, and spirits), tobacco smoking history, leisure-time, physical activity, social class, and age. During 16 years, 1985-1986 to end of 2001, 197 subjects (6.5%) died due to IHD, and 1,204 (39.8%) from all causes. Among non-O phenotypes (A, B, and AB......) significantly fewer men who died due to IHD were wine consumers, 43.9% versus 55.7%, P ... analysis, the hazard ratio (HR) (95% confidence limit) for men drinking up to 8 beverages/wk was 0.5 (0.3-1.02), and among men consuming > 8 beverages/wk (the highest quintile) the HR was 0.3 (0.2-0.8). P wine intake with IHD mortality was slightly...

  20. Comparative frequency and allelic distribution of ABO and Rh (D blood groups of major tribal communities of southern Bangladesh with general population and their determinants

    Gourab Dewan

    2015-04-01

    Results: Collectively all tribal had distinct ABO phenotypic frequency (B > A > O > AB which is different from Bengali population (O > B > A > AB. Tripura’s showed a unique pattern of A > B > AB > O. Overall tribal had higher frequency of B and AB group (P  B > A in all study groups except Tripura (A > O > B. Rhesus negative group was very uncommon in study groups. Data among tribal suggest their common origin as well as drift from original population due to possible founder effect among Tripura’s. Despite being malaria endemic zone the protective group O was less (P < 0.0001 among native tribal (23% and high among migrating Bengali (34%.

  1. Strain effects on the spin polarized electron gas in ABO3/SrTiO3 (A = Pr, Nd and B = Al, Ga) heterostructures

    Nazir, Safdar

    2013-04-11

    The spin polarized two dimensional electron gas in the correlated ABO3/SrTiO3 (A = Pr, Nd and B = Al, Ga) heterostructures is investigated by ab-initio calculations using density functional theory. Structural relaxation shows a strong buckling at and near the TiO2 terminated n-type interface (IFs) due to significant TiO6 octahedral distortions. We find in all cases, metallic states in a very narrow region of the SrTiO3, in agreement with experimental results. We demonstrate that the interface magnetism strongly reacts to the magnitude of the lattice strain. The orbital occupations and, hence, the charge carrier density change systematically as a function of the lattice mismatch between the component materials.

  2. Genetic and mechanistic evaluation for the mixed-field agglutination in B3 blood type with IVS3+5G>A ABO gene mutation.

    Ding-Ping Chen

    Full Text Available BACKGROUND: The ABO blood type B(3 is the most common B subtype in the Chinese population with a frequency of 1/900. Although IVS3+5G>A (rs55852701 mutation of B gene has been shown to associate with the development of B(3 blood type, genetic and mechanistic evaluation for the unique mixed-field agglutination phenotype has not yet been completely addressed. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we analyzed 16 cases of confirmed B(3 individuals and found that IVS3+5G>A attributes to all cases of B(3. RT-PCR analyses revealed the presence of at least 7 types of aberrant B(3 splicing transcripts with most of the transcripts causing early termination and producing non-functional protein during translation. The splicing transcript without exon 3 that was predicted to generate functional B(3 glycosyltransferase lacking 19 amino acids at the N-terminal segment constituted only 0.9% of the splicing transcripts. Expression of the B(3 cDNA with exon 3 deletion in the K562 erythroleukemia cells revealed that the B(3 glycosyltransferase had only 40% of B(1 activity in converting H antigen to B antigen. Notably, the typical mixed-field agglutination of B(3-RBCs can be mimicked by adding anti-B antibody to the K562-B(3 cells. CONCLUSIONS/SIGNIFICANCE: This study thereby demonstrates that both aberrant splicing of B transcripts and the reduced B(3 glycosyltransferase activity contribute to weak B expression and the mixed-field agglutination of B(3, adding to the complexity for the regulatory mechanisms of ABO gene expression.

  3. Linking self-incompatibility, dichogamy, and flowering synchrony in two Euphorbia species: alternative mechanisms for avoiding self-fertilization?

    Eduardo Narbona

    Full Text Available BACKGROUND: Plant species have several mechanisms to avoid selfing such as dichogamy or a self-incompatibility response. Dichogamy in a single flower may reduce autogamy but, to avoid geitonogamy, plants must show flowering synchronization among all their flowers (i.e. synchronous dichogamy. It is hypothesized that one species would not simultaneously show synchronous dichogamy and self-incompatibility because they are redundant mechanisms to reduce selfing; however, this has not been accurately assessed. METHODOLOGY/PRINCIPAL FINDINGS: This expectation was tested over two years in two natural populations of the closely related Mediterranean spurges Euphorbia boetica and E. nicaeensis, which completely avoid autogamy by protogyny at the cyathia level. Both spurges showed a high population synchrony (Z<79, and their inflorescences flower synchronously. In E. nicaeensis, there was no overlap among the cyathia in anthesis of successive inflorescence levels and the overlap between sexual phases of cyathia of the same inflorescence level was uncommon (4-16%. In contrast, E. boetica showed a high overlap among consecutive inflorescence levels (74-93% and between sexual phases of cyathia of the same inflorescence level (48-80%. The flowering pattern of both spurges was consistent in the two populations and over the two successive years. A hand-pollination experiment demonstrated that E. nicaeensis was strictly self-compatible whereas E. boetica was partially self-incompatible. CONCLUSIONS/SIGNIFICANCE: We propose that the complex pattern of synchronized protogyny in E. nicaeensis prevents geitonogamous crosses and, consequently, avoids selfing and inbreeding depression. In E. boetica, a high probability of geitonogamous crosses may occur but, alternatively, this plant escapes selfing through a self-incompatibility response. We posit that synchronous dichogamy and physiological self-incompatibility do not co-occur in the same species because each

  4. Complejo agnatia holoprosencefalia: informe de caso

    Fernando Suárez-Obando

    2007-09-01

    Full Text Available Se presenta un caso de complejo agnatia holoprosencefalia y se realiza una revisión de la literatura, en relación con la compleja etiología genética y embriológica de este conjunto de malformaciones mayores de la cara y el sistema nervioso central. Se trata del primer caso que se informa en la literatura colombiana.

  5. Interactions and incompatibilities of pharmaceutical excipients with active pharmaceutical ingredients: a comprehensive review.

    Sonali S. Bharate

    2010-12-01

    Full Text Available Studies of active drug/excipient compatibility represent an important phase in the preformulation stage of the development of all dosage forms. The potential physical and chemical interactions between drugs and excipients can affect the chemical nature, the stability and bioavailability of drugs and, consequently, their therapeutic efficacy and safety. The present review covers the literaturereports of interaction and incompatibilities of commonly used pharmaceutical excipients with different active pharmaceutical ingredients in solid dosage forms. Examples of active drug/excipientinteractions, such as transacylation, the Maillard browning reaction, acid base reactions and physical changes are discussed for different active pharmaceutical ingredients belonging to different therapeuticcategories viz antiviral, anti-inflammatory, antidiabetic, antihypertensive, anti-convulsant, antibiotic,bronchodialator, antimalarial, antiemetic, antiamoebic, antipsychotic, antidepressant, anticancer, anticoagulant and sedative/hypnotic drugs and vitamins. Once the solid-state reactions of a pharmaceutical system are understood, the necessary steps can be taken to avoid reactivity and improve the stability of drug substances and products.

  6. Dislocations via incompatibilities in phase-field models of microstructure evolution

    Gröger, R.; Marchand, B.; Lookman, T.

    2016-08-01

    We develop a phase-field model that describes the elastic distortion of a ferroelastic material with cubic anisotropy due to an arbitrary dislocation network and a uniform external load. The dislocation network is characterized using the Nye tensor and enters the formulation via a set of incompatibility constraints for the internal strain field. The long-range elastic response of the material is obtained by minimization of the free energy that accounts for higher-order terms of the order parameters and symmetry-adapted strain gradients. The influence of dislocations on the microstructure is studied using a static equilibrium analysis of a material without dislocations and with a random array of parallel edge dislocations. A minimal continuum dislocation dynamics is then used to investigate the simultaneous evolution of the network of geometrically necessary dislocations and the internal strain field. The model developed here is directly applicable to single-phase cubic crystals with an arbitrary degree of anisotropy as well as to ferroelastic materials undergoing temperature-driven cubic-to-tetragonal phase transitions.

  7. Docetaxel epimerization in silicone films: a case of drug excipient incompatibility.

    Mohsin, Shaikh; Arellano, Ian Harvey; Choudhury, Namita Roy; Garg, Sanjay

    2014-10-01

    Docetaxel (DTX) is an anti-cancer compound derived from 10-deacetyl baccatin III which is indicated for treatment of breast, lung, prostate, gastro-esophageal, and head and neck cancers. Epimerization of DTX at the C-7 hydroxyl position has intrigued chemists and has been implicated in loss of potency, as well as in the development of resistance in tumour cells. For localized controlled delivery of this agent, silicone films were prepared from a commercially available silicone kit. High levels of epimeric degradants were unexpectedly found in the in vitro release media. Herein, we discuss this anomalous DTX degradation to epimeric impurities, and discuss the possible reasons for degradation. Systematic stability studies were performed on the release media and the silicone kit components. It was found that release media and tin-based catalyst present in the silicone kit could be responsible for the epimeric conversion. This unusual case of chemical incompatibility can affect product performance and can even lead to development of resistance in tumour cells towards DTX. PMID:24574150

  8. Incompatibility studies by high performance thin-layer chromatography: In case of curcumin

    Alankar Shrivastava

    2013-01-01

    Full Text Available Curcumin[1,7-bis (4-hydroxy-3-methoxy-phenyl hepta-1, 6-diene-3, 5-dione] is one of the component present in the turmeric. Curcumin has been in use for its medicinal benefits since centuries and its therapeutic potential is continuously explored through various researchers throughout the world. To investigate the interaction of curcumin with commonly used excipients such as microcrystalline cellulose, starch, colloidal silica, talc, ascorbic acid, lactose, ethyl cellulose (EC, sodium carboxymethylcellulose (Na-CMC, hydroxyl propyl methyl cellulose and magnesium stearate. High performance thin-layer chromatography (HPTLC is commonly used technique for the determination of phytoconstituents, but its application in incompatibility studies is still not investigated. Thus, we initiated our study with HPTLC followed by Fourier transform infrared and differential scanning calorimetry. Since interaction of curcumin with ascorbic acid, EC, Na-CMC and Mg-stearate confirmed by all three techniques these four excipients should be avoided during the formulation development of curcumin. The presented study also establishes HPTLC′s usefulness in such interaction studies.

  9. Heterosis of Double Low Self-incompatibility in Oilseed Rape (Brassica napus L.)

    SHEN Jin-xiong; FU Ting-dong; YANG Guang-sheng

    2002-01-01

    66 F1 hybrids, produced by 3 double low self-incompatible lines and 22 varieties with a North Carolina Ⅱ (NCⅡ) crossing design, were tested for their heterosis in Wuhan, China during two growing seasons from 1999- 2001. The results showed that significant differences were found between F1s and their parents for yield per plant and seed oil content. Mid-parent heterosis of these two characters ranged from 5.50%-64.11% and from 1.55% -7.44% respectively. Heterosis for seed yield per plant was greater than that of seed oil content. For yield components, heterosis of total number of siliques per plant was the highest, followed by seed number per silique and 1 000 seeds weight. Significant genotype-by-year interaction was found for seed yield per plant. Results from correlation and combining ability analysis indicated that parental effects on its F1 hybrid depended on characters, seed yield per plant was affected by both additive and non-additive effects, and seed oil content was affected mainly by additive effect. When designing hybrid programme, parents might be selected by GCAs and variances of SCAs of parents for the characters affected by both additive and non-additive effects, and by the sum of GCAs of female and male parents for the characters mainly affected by additive effects.

  10. Development of Cuscuta species on a partially incompatible host: induction of xylem transfer cells.

    Christensen, Nynne M; Dörr, Inge; Hansen, M; van der Kooij, T A W; Schulz, A

    2003-03-01

    The growth of dodders, Cuscuta reflexa and Cuscuta japonica, on the partially incompatible host poinsettia ( Euphorbia pulcherrima) is studied. Poinsettia responds by bark growths to the formation of the dodder haustoria and prevents dodder from obtaining normal growth. The growth instead becomes extremely branched, coral-like, and dodder lacks the ability to form haustoria. After a period of coral-like growth, long shoots sprout, resembling the normal growth. These long shoots mark an ending phase for dodder, which dies shortly after without having flowered. During the coral-like growth phase, dodder develops transfer cells in the parenchyma cells bordering the vessels of the xylem in the shoot. The transfer cells have not been observed when dodder is grown on the compatible host Pelargonium zonale. A coral-like growth phase has also been observed at the establishing phase when dodder is grown in vitro on agar; later a more normal growth form takes over. In this coral phase, xylem transfer cells are also developed. The fluorochromes carboxyfluorescein and Texas Red were loaded into the host in the phloem and xylem, respectively, and detection of these fluorochromes in the dodder stem indicated that a functional haustorial contact developed for both vascular systems. The results show that Cuscutaspp. have the genetic ability to develop xylem transfer cells and use this in response to developmental stress. PMID:12664277

  11. Hybrid Incompatibilities, Local Adaptation, and the Genomic Distribution of Natural Introgression between Species.

    Muirhead, Christina A; Presgraves, Daven C

    2016-02-01

    Under allopatric speciation, geographic barriers eliminate gene flow between eventual species at all loci in the genome simultaneously. There is increasing evidence, however, that speciation can be complex, with some loci experiencing gene flow during speciation or during bouts of secondary contact. In taxa with heteromorphic sex chromosomes-birds, butterflies, mammals, and Drosophila-the X (or Z) chromosome generally shows reduced levels of gene flow compared to autosomes. To investigate why, we develop population genetic models of secondary contact and gene flow at a neutral locus that is genetically linked to selected loci involved in hybrid incompatibilities and/or local adaptation. Using models that assume weak migration and strong selection, we compare gene flow at X-linked versus autosomal neutral loci as a function of linkage, dominance, sex-specific selection, and sex-specific recombination. For most cases, gene flow at neutral loci on the X is reduced relative to autosomes, as the greater efficacy of hemizygous selection in XY hybrids reduces the opportunity for neutral migrant alleles to escape their genetically linked, locally disfavored alleles via recombination. There are some circumstances, however, involving sex-limited selection and sex-limited recombination that allow neutral loci on the X to introgress more readily than those on autosomes. PMID:26807751

  12. Crossability and evaluation of incompatibility barriers in crosses between Capsicum species

    Kellen Coutinho Martins

    2015-08-01

    Full Text Available The objectives of this study were to estimate the crossability rate in combinations of and assess the occurrence of incompatibility barriers between Capsicum species. The species C. annuum, C. chinense, C. frutescens, C. baccatum var. pendulum, and C. baccatum var. baccatum were crossed and the number of hand-pollinations and of resulting fruits and plants was registered. The resulting hybrids were evaluated for their pollen viability and in the crosses that produced no hybrids, the in vivo germination of pollen grains was assessed. Some crosses generated hybrids, while others produced no fruit or, despite generating fruits, no plants grew from them. The crossability rate between species of the complex C. annuum and C. baccatum ranged from 2.2% to 3.7%, and was 14.6% between species of the complex C. annuum. A pre-fertilization barrier was observed in the non-germination of pollen grains, as well as post-fertilization barriers consisting of embryo death, lack of vigor and hybrid sterility

  13. Reproductive isolation is mediated by pollen incompatibility in sympatric populations of two Arnebia species.

    Wang, Lin-Lin; Zhang, Chan; Tian, Bin; Sun, Xu-Dong; Guo, Wen; Zhang, Ting-Feng; Yang, Yong-Ping; Duan, Yuan-Wen

    2015-12-01

    To explore uncertain aspects of the processes that maintain species boundaries, we evaluated contributions of pre- and postpollination reproductive isolation mechanisms in sympatric populations of Arnebia guttata and A. szechenyi. For this, we investigated their phylogenetic relationships, traits, microenvironments, pollinator visits, action of natural selection on floral traits, and the outcome of hand pollination between the two species. Phylogenetic analysis indicates that A. szechenyi is a derived species that could be closely related to A. guttata, and both could be diploid species. Arnebia guttata flowers have larger parts than A. szechenyi flowers, but smaller nectar guides. Soil supporting A. szechenyi had higher water contents than soil supporting neighboring populations of A. guttata (in accordance with their geographical distributions). The pollinators shared by the two species preferred A. szechenyi flowers, but interspecific visitations were frequent. We found evidence of conflicting selection pressures on floral tube length, flower diameter and nectar guide size mediated via male fitness, and on flower diameter and floral tube diameter via female fitness. Hand-pollination experiments indicate complete pollen incompatibility between the two species. Our results suggest that postpollination prezygotic mechanisms are largely responsible for reproductive isolation of sympatric populations of the two Arnebia species. PMID:26811758

  14. Vegetative and generative maintenance of self-incompatibility in six accessions of German chamomile.

    Faehnrich, Bettina; Wagner, Sarah; Franz, Chlodwig

    2016-06-01

    Self-incompatible (SI) plants are able to form ideal mother lines for hybrid crossing in hermaphroditic plants, assuring fertilization from the desired father line. To find out suitable ways to maintain SI was the aim of this study. Among 220 plants of German chamomile (Matricaria recutita (L.) Rauschert) within six accessions SI-genotypes were selected. SI was determined as staying seedless in three flower heads per plant. Initial SI-plants formed the basic paternal generation (P1) of i) maintaining the same genotypes over six months and repeating seed set analysis (P2) and of ii) conducting crossings in three versions (SI × SI, SI × NSI (not SI evaluated plants) and NSI × SI), thereby producing the F1 population. F1 exhibited 78% SI and P2 62% SI, indicating a higher environmental than genetic influence on SI. But heritability, calculated from the results of SI × SI crossings, showed high values (h(2) = 0.71). Within generative propagation, the influence of generation/crossing version was highly significant (p = 0.001) and the cultivar 'Degumille' explored the highest value of SI (86%) after SI × NSI crossings. Therefore, the intra-cultivar combination of 'Degumille' SI mother plants crossed with NSI father plants can be recommended as the most promising version to maintain SI in chamomile. PMID:27436956

  15. Bell-Boole Inequality: Nonlocality or Probabilistic Incompatibility of Random Variables?

    Andrei Khrennikov

    2008-03-01

    Full Text Available The main aim of this report is to inform the quantum information community about investigations on the problem of probabilistic compatibility of a family of random variables: a possibility to realize such a family on the basis of a single probability measure (to construct a single Kolmogorov probability space. These investigations were started hundred of years ago by J. Boole (who invented Boolean algebras. The complete solution of the problem was obtained by Soviet mathematician Vorobjev in 60th. Surprisingly probabilists and statisticians obtained inequalities for probabilities and correlations among which one can find the famous Bell’s inequality and its generalizations. Such inequalities appeared simply as constraints for probabilistic compatibility. In this framework one can not see a priori any link to such problems as nonlocality and “death of reality” which are typically linked to Bell’s type inequalities in physical literature. We analyze the difference between positions of mathematicians and quantum physicists. In particular, we found that one of the most reasonable explanations of probabilistic incompatibility is mixing in Bell’s type inequalities statistical data from a number of experiments performed under different experimental contexts.

  16. Endocarditis infecciosa activa: 152 casos

    Lucía R. Kazelian

    2012-04-01

    Full Text Available La endocarditis infecciosa es una enfermedad de baja incidencia que en las últimas décadas mostró modificaciones respecto de su presentación, posibilidad diagnóstica y tratamiento. A pesar de estos avances, la mortalidad hospitalaria sigue siendo muy elevada. Nuestro objetivo fue analizar las características de los pacientes con endocarditis infecciosa activa y su relación con la mortalidad hospitalaria a lo largo de 16 años. Se realizó un registro prospectivo entre 1994 y 2010 de pacientes ingresados con endocarditis. Se analizaron características clínicas, evolución y tratamiento y se registraron los eventos intrahospitalarios. Ingresaron 152 pacientes, 64.5% varones, edad 45 ± 16 años, las causas más frecuentes de cardiopatía de base fueron: congénita 32 (21% y reumática 20 (13.2%. Los motivos de internación fueron síndrome febril 116 (76.3% e insuficiencia cardíaca 61 (40.1%. Se identificó el agente infeccioso en 106 (69.7% de los casos, el más frecuente fue Streptococcus viridans. El ecocardiograma mostró vegetaciones en 123 (80.9% de los pacientes y 88 (57.8% presentaron complicaciones durante su internación, siendo la más frecuente la insuficiencia cardíaca. Se indicó tratamiento quirúrgico en 96 (63.1% de los casos, fundamentalmente por insuficiencia cardíaca en 66. La mortalidad hospitalaria global fue 46 (30.2%. El desarrollo de complicaciones en la internación, la indicación de cirugía y la presencia de insuficiencia cardíaca refractaria al tratamiento fueron predictores independientes de mortalidad hospitalaria, mientras que la presencia de vegetaciones resultó un predictor independiente de mejor supervivencia. La identificación temprana de estos predictores descriptos podría ayudar a mejorar los resultados.

  17. HLA-DP and bone marrow transplantation: DP-incompatibility and severe acute graft versus host disease

    Ødum, Niels; Platz, P; Jakobsen, B K;

    1987-01-01

    Thirteen recipients of HLA-haploidentical, DR compatible bone marrow (BM) and the corresponding BM donors were HLA-DP typed using primed lymphocyte typing (PLT). Severe acute GVHD (greater than or equal to grade 2) developed within 3 months after BM-transplantation in all of eight recipients of DP...... incompatible BM, but in none of five recipients of DP-compatible BM. This difference was highly significant (p less than 0.001, Fisher's exact test). Moreover, severe acute GVHD was significantly increased in recipients of haploidentical, DR compatible, but DP incompatible BM as compared to severe acute GVHD...... in 88 recipients of HLA-identical BM (p less than 0.0001). In contrast, there was no difference in acute GVHD between recipients of haploidentical, DR and DP compatible BM and recipients of HLA-identical BM. The data presented here provide strong evidence for the first time that HLA-DP antigens play...

  18. Distinct effects of pollinator dependence and self-incompatibility on pollen limitation in South African biodiversity hotspots.

    Rodger, James G; Ellis, Allan G

    2016-06-01

    Global synthesis indicates that limitation of plant fecundity by pollen receipt (pollen limitation) is positively related to regional plant diversity and is higher for self-incompatible than self-compatible species. While self-incompatible species are always dependent on pollinating agents, self-compatible species may be pollinator-dependent or autofertile. This should cause variation in pollen limitation among self-compatible species, with lower pollen limitation in autofertile species because they do not depend on pollinators. We hypothesized that the intensity of pollen limitation in self-incompatible compared with pollinator-dependent self-compatible species should depend on whether pollen limitation is determined more by quantity than quality of pollen received. We compared pollen limitation between these three groups using a dataset of 70 biotically pollinated species from biodiverse regions of South Africa. Comparison with a global dataset indicated that pollen limitation in the South African biodiversity hotspots was generally comparable to other regions, despite expectations of higher pollen limitation based on the global plant diversity-pollen limitation relationship. Pollen limitation was lowest for autofertile species, as expected. It was also higher for pollinator-dependent self-compatible species than self-incompatible species, consistent with increased pollen-quality limitation in the former group due to negative consequences of pollinator-mediated self-pollination. However, there was a higher frequency of plants with zygomorphic flowers, which were also more pollen-limited, among pollinator-dependent self-compatible species. Thus, we could not attribute this difference in pollen limitation exclusively to a difference in pollen quality. Nevertheless, our results indicate that comparative studies should control for both pollinator dependence and self-incompatiblity when evaluating effects of other factors on pollen limitation. PMID:27277954

  19. Molecular responses of Lotus japonicus to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica

    Hiraoka, Yukihiro; Ueda, Hiroaki; Sugimoto, Yukihiro

    2008-01-01

    Lotus japonicus genes responsive to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica were isolated by using the suppression subtractive hybridization (SSH) strategy. O. aegyptiaca and S. hermonthica parasitism specifically induced the expression of genes involved in jasmonic acid (JA) biosynthesis and phytoalexin biosynthesis, respectively. Nodulation-related genes were almost exclusively found among the Orobanche-induced genes. Tempora...

  20. Newspaper and personal life of journalist women in eastern Spain: the professional aspects of the incompatibility with family life

    Papí Gálvez, Natalia; Feliu García, Emilio; Frau Llinares, María José (coord.)

    2005-01-01

    According to IFJ and UNESCO the principal obstacles of the journalism practice are: the patriarchy model of the medias and the family demands. This paper aims to explore the professional aspects of incompatibility with a family life on journalism. It focus on the newspapers established in eastern Spain. The results of this article is based on two set of information: statistics and documentary revision (Census- 2001, Surveys of CIS) and interviews in depth to journalist women. The work fie...

  1. Wolbachia Density and Cytoplasmic Incompatibility in Aedes albopictus: Concerns with Using Artificial Wolbachia Infection as a Vector Suppression Tool

    Maurizio Calvitti; Francesca Marini; Angiola Desiderio; Arianna Puggioli; Riccardo Moretti

    2015-01-01

    The mosquito Aedes albopictusi is a competent vector of harmful human pathogens, including viruses causing dengue and chikungunya. Cytoplasmic incompatibility (CI) induced by endosymbiotic Wolbachia can be used to produce functionally sterile males that can be released in the field as a suppression tool against this mosquito. Because the available sexing methods are not efficient enough to avoid unintentional release of a few transinfected females, we assessed the CI pattern in crosses betwee...

  2. Pyrosequencing-Based Transcriptome Analysis of the Asian Rice Gall Midge Reveals Differential Response during Compatible and Incompatible Interaction

    Jagadish S. Bentur; Suresh Nair; Archana Tomar; Javaregowda Nagaraju; Deepak Kumar Sinha

    2012-01-01

    The Asian rice gall midge (Orseolia oryzae) is a major pest responsible for immense loss in rice productivity. Currently, very little knowledge exists with regard to this insect at the molecular level. The present study was initiated with the aim of developing molecular resources as well as identifying alterations at the transcriptome level in the gall midge maggots that are in a compatible (SH) or in an incompatible interaction (RH) with their rice host. Roche 454 pyrosequencing strategy was...

  3. Necessity of Combining Mutually Incompatible Perspectives in the Construction of a Global View Quantum Probability and Signal Analysis

    Aerts, S; Schroeck, F E; Aerts, Sven; Aerts, Diederik; Schroeck, Franklin E.

    2005-01-01

    The scientific fields of quantum mechanics and signal-analysis originated within different settings, aimed at different goals and started from different scientific paradigms. Yet the development of the two subjects has become increasingly intertwined. We argue that these similarities are rooted in the fact that both fields of scientific inquiry had to deal with finding a single description for a phenomenon that yields complete information about itself only when we consider mutually incompatible accounts of that phenomenon.

  4. Transcription of ColE1Ap mbeC induced by conjugative plasmids from twelve different incompatibility groups.

    Selvaratnam, S; Gealt, M A

    1993-01-01

    Although nonconjugative mobilizable plasmids require helping functions of conjugative plasmids in order to be mobilized into recipients, at least some genes from the nonconjugative plasmids may be induced to assist in the DNA transfer process. Conjugative plasmids from 12 different incompatibility groups mobilized the nonconjugative plasmid ColE1Ap between Escherichia coli strains. Introduction of any of the conjugative plasmids into the ColE1Ap-containing strain resulted in an induction of m...

  5. Transcriptome analysis reveals self-incompatibility in the tea plant (Camellia sinensis) might be under gametophytic control

    Zhang, Cheng-Cai; Wang, Li-Yuan; Wei, Kang; Wu, Li-Yun; Li, Hai-Lin; Zhang, Fen; Cheng, Hao; Ni, De-Jiang

    2016-01-01

    Background Self-incompatibility (SI) is under genetic control and prevents inbreeding depression in angiosperms. SI mechanisms are quite complicated and still poorly understood in many plants. Tea (Camellia sinensis L.) belonging to the family of Theaceae, exhibits high levels of SI and high heterozygosity. Uncovering the molecular basis of SI of the tea plant may enhance breeding and simplify genomics research for the whole family. Results The growth of pollen tubes following selfing and cro...

  6. Incest versus abstinence: reproductive trade-offs between mate limitation and progeny fitness in a self-incompatible invasive plant

    Pierson, Jennifer C; Swain, Stephen M; Young, Andrew G

    2013-01-01

    Plant mating systems represent an evolutionary and ecological trade-off between reproductive assurance through selfing and maximizing progeny fitness through outbreeding. However, many plants with sporophytic self-incompatibility systems exhibit dominance interactions at the S-locus that allow biparental inbreeding, thereby facilitating mating between individuals that share alleles at the S-locus. We investigated this trade-off by estimating mate availability and biparental inbreeding depress...

  7. Lack of Association between ABO, PPAP2B, ADAMST7, PIK3CG, and EDNRA and Carotid Intima-Media Thickness, Carotid Plaques, and Cardiovascular Disease in Patients with Rheumatoid Arthritis

    Raquel López-Mejías; Fernanda Genre; Mercedes García-Bermúdez; Begoña Ubilla; Santos Castañeda; Javier Llorca; Carlos González-Juanatey; Alfonso Corrales; Miranda-Filloy, José A.; Trinitario Pina; Carmen Gómez-Vaquero; Luis Rodríguez-Rodríguez; Benjamín Fernández-Gutiérrez; Alejandro Balsa; Dora Pascual-Salcedo

    2014-01-01

    Introduction. Rheumatoid arthritis (RA) is a polygenic disease associated with accelerated atherosclerosis and increased cardiovascular (CV) mortality. Recent studies have identified the ABO rs579459, PPAP2B rs17114036, and ADAMTS7 rs3825807 polymorphisms as genetic variants associated with coronary artery disease and the PIK3CG rs17398575 and EDNRA rs1878406 polymorphisms as the most significant signals related to the presence of carotid plaque in nonrheumatic Caucasian individuals. Accordin...

  8. Genetic diversity of hemoglobinopathies, G6PD deficiency, and ABO and Rhesus blood groups in two isolates of a primitive Kharia Tribe in Sundargarh District of Northwestern Orissa, India

    Balgir, R. S.

    2010-01-01

    Tribal communities constitute about 8.2% of the total population of India. Their health needs are even larger than elsewhere in India; this study investigates the genetic diversity in relation to hemoglobinopathies, G6PD deficiency and, ABO and Rhesus (D) blood groups in two sects, i.e. Dudh (converted Christian) and Dhelki (Hinduised) Kharia, a primitive tribe in Sundargarh district of Orissa in Central-Eastern India. A randomized screening of 767 Kharia tribals (377 males and 390 females) b...

  9. Prognostic role ofthe ABO blood types inChinese patients withcuratively resected non-small cell lung cancer:a retrospective analysis of1601 cases ata single cancer center

    NingLi; SiYuWang; MiaoXu; ChaoFengLi; WeiOu; BaoXiaoWang; SongLiangZhang; PengFeiXu; ChengYuan; QunAiHuang

    2015-01-01

    Background:A positive association between the ABO blood types and survival has been suggested in several malig‑nancies. The aim of this study was to assess the role of the ABO blood types in predicting the prognosis of Chinese patients with curatively resected non‑small cell lung cancer (NSCLC). Methods:We retrospectively analyzed 1601 consecutive Chinese patients who underwent curative surgery for NSCLC between January 1, 2005 and December 31, 2009. The relationship between the ABO blood types and survival was investigated. In addition, univariate and multivariate analyses were performed. Results:Group 1 (patients with the blood type O or B) had signiifcantly prolonged overall survival (OS) compared with group 2 (patients with the blood type A or AB), with a median OS of 74.9months versus 61.5months [hazard ratio (HR) 0.83; 95% conifdence interval (CI) 0.72–0.96;P=0.015]. Additionally, group 1 had signiifcantly longer dis‑ease‑free survival (DFS; HR 0.86; 95% CI 0.76–0.98;P=0.022) and locoregional relapse‑free survival (LRFS; HR 0.79; 95%CI 0.64–0.98;P=0.024) than group 2. The association was not signiifcantly modiifed by other risk factors for NSCLC, including smoking status, pathologic tumor‑node‑metastasis stage, pT category, pN category, and chemotherapy. Conclusions:There is an association between the ABO blood types and the survival of Chinese patients with resected NSCLC. Patients with the blood type O or B had signiifcantly prolonged OS, DFS, and LRFS compared with those with the blood type A or AB.

  10. Comparative analysis of transcriptomic and hormonal responses to compatible and incompatible plant-virus interactions that lead to cell death.

    Pacheco, Remedios; García-Marcos, Alberto; Manzano, Aranzazu; de Lacoba, Mario García; Camañes, Gemma; García-Agustín, Pilar; Díaz-Ruíz, José Ramón; Tenllado, Francisco

    2012-05-01

    Hypersensitive response-related programmed cell death (PCD) has been extensively analyzed in various plant-virus interactions. However, little is known about the changes in gene expression and phytohormone levels associated with cell death caused by compatible viruses. The synergistic interaction of Potato virus X (PVX) with a number of Potyvirus spp. results in increased symptoms that lead to systemic necrosis (SN) in Nicotiana benthamiana. Here, we show that SN induced by a PVX recombinant virus expressing a potyviral helper component-proteinase (HC-Pro) gene is associated with PCD. We have also compared transcriptomic and hormonal changes that occur in response to a compatible synergistic virus interaction that leads to SN, a systemic incompatible interaction conferred by the Tobacco mosaic virus-resistance gene N, and a PCD response conditioned by depletion of proteasome function. Our analysis indicates that the SN response clusters with the incompatible response by the similarity of their overall gene expression profiles. However, the expression profiles of both defense-related genes and hormone-responsive genes, and also the relative accumulation of several hormones in response to SN, relate more closely to the response to depletion of proteasome function than to that elicited by the incompatible interaction. This suggests a potential contribution of proteasome dysfunction to the increased pathogenicity observed in PVX-Potyvirus mixed infections. Furthermore, silencing of coronatine insensitive 1, a gene involved in jasmonate perception, in N. benthamiana accelerated cell death induced by PVX expressing HC-Pro. PMID:22273391

  11. Inbreeding depression in Solanum carolinense (Solanaceae, a species with a plastic self-incompatibility response

    Keser Lidewij H

    2008-01-01

    Full Text Available Abstract Background Solanum carolinense (horsenettle is a highly successful weed with a gametophytic self-incompatibility (SI system. Previous studies reveal that the strength of SI in S. carolinense is a plastic trait, associated with particular S-alleles. The importance of this variation in self-fertility on the ability of horsenettle to found and establish new populations will depend, to a large extent, on the magnitude of inbreeding depression. We performed a series of greenhouse and field experiments to determine the magnitude of inbreeding depression in S. carolinense, whether inbreeding depression varies by family, and whether the estimates of inbreeding depression vary under field and greenhouse conditions. We performed a series of controlled self- and cross-pollinations on 16 genets collected from a large population in Pennsylvania to obtain progeny with different levels of inbreeding. We grew the selfed and outcrossed progeny in the greenhouse and under field conditions and recorded various measures of growth and reproductive output. Results In the greenhouse study we found (1 a reduction in flower, fruit and seed production per fruit in inbred (selfed progeny when compared to outbred (outcrossed progeny; (2 a reduction in growth of resprouts obtained from rhizome cuttings of selfed progeny; and (3 an increase in the ability to self-fertilize in the selfed progeny. In the field, we found that (1 outcrossed progeny produced more leaves than their selfed siblings; (2 herbivory seems to add little to inbreeding depression; and (3 outcrossed plants grew faster and were able to set more fruits than selfed plants. Conclusion Solanum carolinense experiences low levels of inbreeding depression under greenhouse conditions and slightly more inbreeding depression under our field conditions. The combined effects of low levels of inbreeding depression and plasticity in the strength of SI suggest that the production of selfed progeny may play an

  12. Explanatory Pluralism and the (Dis)Unity of Science: The Argument from Incompatible Counterfactual Consequences.

    Gijsbers, Victor

    2016-01-01

    What is the relationship between different sciences or research approaches that deal with the same phenomena, for instance, with the phenomena of the human mind? Answers to this question range from a monist perspective according to which one of these approaches is privileged over the others, through an integrationist perspective according to which they must strive to form a unity greater than the sum of its parts, to an isolationist perspective according to which each of them has its own autonomous sphere of validity. In order to assess these perspectives in this article, I discuss the debates about the unity of science and about explanatory pluralism. The most pressing issue turns out to be the choice between the integrative and the isolationist perspective: the question is whether the integrative tendencies in science should be fully indulged in or whether they should be held in check by acknowledging that a certain amount of isolation is necessary. I argue that the issue can be further distilled into the question of whether two true explanations of the same fact can ever fail to be combinable into one single explanation. I show that this can indeed be the case, namely, when the explanations have incompatible counterfactual consequences, something that is often the case when we try to combine explanations from different sciences or research approaches. These approaches thus embody perspectives on the world that are to a certain extent autonomous. This leads to the conclusion that although interdisciplinarity may have many advantages, we should not take the project of integration too far. At the end of the day, the different research approaches with their different perspectives and insights must remain precisely that: different and somewhat disunified. PMID:27014099

  13. A Genome-wide hybrid incompatibility landscape between Caenorhabditis briggsae and C. nigoni.

    Yu Bi

    2015-02-01

    Full Text Available Systematic characterization of ẖybrid incompatibility (HI between related species remains the key to understanding speciation. The genetic basis of HI has been intensively studied in Drosophila species, but remains largely unknown in other species, including nematodes, which is mainly due to the lack of a sister species with which C. elegans can mate and produce viable progeny. The recent discovery of a C. briggsae sister species, C. nigoni, has opened up the possibility of dissecting the genetic basis of HI in nematode species. However, the paucity of dominant and visible marker prevents the efficient mapping of HI loci between the two species. To elucidate the genetic basis of speciation in nematode species, we first generated 96 chromosomally integrated GFP markers in the C. briggsae genome and mapped them into the defined locations by PCR and Next-Generation Sequencing (NGS. Aided by the marker, we backcrossed the GFP-associated C. briggsae genomic fragments into C. nigoni for at least 15 generations and produced 111 independent introgressions. The introgression fragments cover most of the C. briggsae genome. We finally dissected the patterns of HI by scoring the embryonic lethality, larval arrest, sex ratio and male sterility for each introgression line, through which we identified pervasive HI loci and produced a genome-wide landscape of HI between the two nematode species, the first of its type for any non-Drosophila species. The HI data not only provided insights into the genetic basis of speciation, but also established a framework for the possible cloning of HI loci between the two nematode species. Furthermore, the data on hybrids confirmed Haldane's rule and suggested the presence of a large X effect in terms of fertility between the two species. Importantly, this work opens a new avenue for studying speciation genetics between nematode species and allows parallel comparison of the HI with that in Drosophila and other species.

  14. Secondary evolution of a self-incompatibility locus in the Brassicaceae genus Leavenworthia.

    Sier-Ching Chantha

    Full Text Available Self-incompatibility (SI is the flowering plant reproductive system in which self pollen tube growth is inhibited, thereby preventing self-fertilization. SI has evolved independently in several different flowering plant lineages. In all Brassicaceae species in which the molecular basis of SI has been investigated in detail, the product of the S-locus receptor kinase (SRK gene functions as receptor in the initial step of the self pollen-rejection pathway, while that of the S-locus cysteine-rich (SCR gene functions as ligand. Here we examine the hypothesis that the S locus in the Brassicaceae genus Leavenworthia is paralogous with the S locus previously characterized in other members of the family. We also test the hypothesis that self-compatibility in this group is based on disruption of the pollen ligand-producing gene. Sequence analysis of the S-locus genes in Leavenworthia, phylogeny of S alleles, gene expression patterns, and comparative genomics analyses provide support for both hypotheses. Of special interest are two genes located in a non-S locus genomic region of Arabidopsis lyrata that exhibit domain structures, sequences, and phylogenetic histories similar to those of the S-locus genes in Leavenworthia, and that also share synteny with these genes. These A. lyrata genes resemble those comprising the A. lyrata S locus, but they do not function in self-recognition. Moreover, they appear to belong to a lineage that diverged from the ancestral Brassicaceae S-locus genes before allelic diversification at the S locus. We hypothesize that there has been neo-functionalization of these S-locus-like genes in the Leavenworthia lineage, resulting in evolution of a separate ligand-receptor system of SI. Our results also provide support for theoretical models that predict that the least constrained pathway to the evolution of self-compatibility is one involving loss of pollen gene function.

  15. Deep venous thrombosis: The valve cusp hypoxia thesis and its incompatibility with modern orthodoxy.

    Malone, P Colm; Agutter, Paul S

    2016-01-01

    The valve cusp hypoxia thesis (VCHT) of the aetiology of deep venous thrombosis (DVT) was adumbrated in this journal in 1977 and fully articulated in 2008, the original hypothesis having been strongly corroborated by experiments published in 1981 and 1984. It presents a unitary account of the pathogenesis of venous thrombosis and embolism that is rooted in the pathophysiological tradition of Hunter, Virchow, Lister, Welch and Aschoff, a tradition traceable back to Harvey. In this paper we summarise the thesis in its mature form, consider its compatibility with recent advances in the DVT field, and ask why it has not yet been assimilated into the mainstream literature, which during the past half century has been dominated by a haematology-orientated 'consensus model'. We identify and discuss seven ways in which the VCHT is incompatible with these mainstream beliefs about the aetiology of venous thrombosis, drawing attention to: (1) the spurious nature of 'Virchow's triad'; (2) the crucial differences between 'venous thrombus' and 'clot'; the facts that (3) venous thrombi form in the valve pockets (VVPs), (4) DVT is not a primarily haematological condition, (5) the so-called 'thrombophilias' are not thrombogenic per se; (6) the conflict between the single unitary aetiology of DVT and the tacit assumption that the condition is 'multicausal'; (7) the inability of anticoagulants to prevent the initiation of venous thrombogenesis, though they do prevent the growth of thrombi to clinically significant size. In discussing point (7), we show that the VCHT indicates new approaches to mechanical prophylaxis against DVT. These approaches are then formulated as experimentally testable hypotheses, and we suggest methods for testing them preclinically using animal trials. PMID:26804599

  16. Wolbachia-induced cytoplasmic incompatibility is associated with decreased Hira expression in male Drosophila.

    Ya Zheng

    Full Text Available BACKGROUND: Wolbachia are obligate endosymbiotic bacteria that infect numerous species of arthropods and nematodes. Wolbachia can induce several reproductive phenotypes in their insect hosts including feminization, male-killing, parthenogenesis and cytoplasmic incompatibility (CI. CI is the most common phenotype and occurs when Wolbachia-infected males mate with uninfected females resulting in no or very low numbers of viable offspring. However, matings between males and females infected with the same strain of Wolbachia result in viable progeny. Despite substantial scientific effort, the molecular mechanisms underlying CI are currently unknown. METHODOLOGY/PRINCIPAL FINDINGS: Gene expression studies were undertaken in Drosophila melanogaster and D. simulans which display differential levels of CI using quantitative RT-PCR. We show that Hira expression is correlated with the induction of CI and occurs in a sex-specific manner. Hira expression is significantly lower in males which induce strong CI when compared to males inducing no CI or Wolbachia-uninfected males. A reduction in Hira expression is also observed in 1-day-old males that induce stronger CI compared to 5-day-old males that induce weak or no CI. In addition, Hira mutated D. melanogaster males mated to uninfected females result in significantly decreased hatch rates comparing with uninfected crosses. Interestingly, wMel-infected females may rescue the hatch rates. An obvious CI phenotype with chromatin bridges are observed in the early embryo resulting from Hira mutant fertilization, which strongly mimics the defects associated with CI. CONCLUSIONS/SIGNIFICANCE: Our results suggest Wolbachia-induced CI in Drosophila occurs due to a reduction in Hira expression in Wolbachia-infected males leading to detrimental effects on sperm fertility resulting in embryo lethality. These results may help determine the underlying mechanism of CI and provide further insight in to the important role

  17. Further insight into reproductive incompatibility between putative cryptic species of the Bemisia tabaci whitefly complex.

    Qin, Li; Pan, Li-Long; Liu, Shu-Sheng

    2016-04-01

    The whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), with its global distribution and extensive genetic diversity, is now known to be a complex of over 35 cryptic species. However, a satisfactory resolution of the systematics of this species complex is yet to be achieved. Here, we designed experiments to examine reproductive compatibility among species with different levels of mitochondrial cytochrome oxidase I (mtCOI) divergence. The data show that putative species with mtCOI divergence of >8% between them consistently exhibited complete reproductive isolation. However, two of the putative species, Asia II 9 and Asia II 3, with mtCOI divergence of 4.47% between them, exhibited near complete reproductive compatibility in one direction of their cross, and partial reproductive compatibility in the other direction. Together with some recent reports on this topic from the literature, our data indicates that, while divergence in the mtCOI sequences provides a valid molecular marker for species delimitation in most clades, more genetic markers and more sophisticated molecular phylogeny will be required to achieve adequate delimitation of all species in this whitefly complex. While many attempts have been made to examine the reproductive compatibility among genetic groups of the B. tabaci complex, our study represents the first effort to conduct crossing experiments with putative species that were chosen with considerations of their genetic divergence. In light of the new data, we discuss the best strategy and protocols to conduct further molecular phylogenetic analysis and crossing trials, in order to reveal the overall pattern of reproductive incompatibility among species of this whitefly complex. PMID:27001484

  18. Vasculitis por Propiltiouracilo: reporte de un caso

    Donato A. Salas-Segura; Juan I Padilla-Cuadra; Juliana Salas-Segura

    2002-01-01

    Se reporta el caso clínico de una paciente femenina de 43 años que presentó dos complicaciones secundarias al uso de propiltiouracilo: vasculitis y agranulocitosis.La vasculitis asociada con el tratamiento antitiroideo es una entidad clínica bien documentada, pero rara, y de un mecanismo patogénico no claro aún. Hay pocos casos reportados de asociación entre la ingesta de propiltiouracilo, la aparición de anticuerpos anticitoplasmáticos antineutrófilos y vasculitis ANCA positiva. Este es prob...

  19. Vasculitis por Propiltiouracilo: reporte de un caso

    Donato A. Salas-Segura

    2002-06-01

    Full Text Available Se reporta el caso clínico de una paciente femenina de 43 años que presentó dos complicaciones secundarias al uso de propiltiouracilo: vasculitis y agranulocitosis.La vasculitis asociada con el tratamiento antitiroideo es una entidad clínica bien documentada, pero rara, y de un mecanismo patogénico no claro aún. Hay pocos casos reportados de asociación entre la ingesta de propiltiouracilo, la aparición de anticuerpos anticitoplasmáticos antineutrófilos y vasculitis ANCA positiva. Este es probablemente uno de ellos.

  20. Estudo de caso: neuropatia auditiva Auditory neuropathy: a study case

    Viviane M. Parra

    2003-03-01

    Full Text Available Atualmente, podemos complementar os resultados encontrados na audiometria convencional com os obtidos nos exames eletrofisiológicos, o que nos permite diagnosticar não apenas uma perda auditiva periférica, mas também diferenciar uma perda auditiva coclear da neural, ou central. Isto nos conduziu a um grupo novo de pacientes que apresentava alteração na sincronia neural com função normal das células ciliadas externas. Esta patologia é conhecida como Neuropatia Auditiva. O objetivo deste estudo é descrever as características audiológicas de um paciente com Neuropatia Auditiva atendido no Centro de Docência e Pesquisa em Fonoaudiologia da Faculdade de Medicina da Universidade de São Paulo (FMUSP, correlacionando os achados com os encontrados na literatura. O caso descrito é de um indivíduo de 24 anos de idade, com o diagnóstico de Neuropatia Auditiva, atendido no ano de 2001 no Centro de Docência e Pesquisa em Fonoaudiologia da FMUSP. Foram realizados anamnese, audiometria tonal e vocal, medidas de imitância acústica, Emissões Otoacústicas (EOAs e Audiometria de Tronco Encefálico (ABR. Observamos no caso estudado a incompatibilidade de resultados entre audiometria tonal e testes de inteligibilidade de fala, com os exames objetivos, EOAs e ABR, onde observou-se perda auditiva com importante alteração nos testes de inteligibilidade de fala, EOAs presentes e ABR anormal. Estes dados sugerem função coclear normal e alteração da sincronia neural.Nowadays, in our clinic, we can complement the conventional audiometry results with electrophysiologic exams. Not only does allow us to diagnose a peripheral hearing loss but also to differentiate cochlear, neural or central hearing loss. It led us to a new group of patients that presented changes in neural synchrony, with normal functions of the outer ear hair cells. This pathology is known as Auditory Neuropathy. Our study purpose is describe the audiologist characteristic of a

  1. Graft incompatibility in plants: Metabolic changes during formation and establishment of the rootstock/scion union with emphasis on Prunus species

    Felipe Gainza

    2015-08-01

    Full Text Available Commercial fruit trees are usually formed by the combination of a rootstock and a scion to broaden the adaptability of scion cultivars to soil and climatic conditions, facilitate agricultural management, and/or increase productivity. In the different cultivated species of the genus Prunus, rootstocks having a wide range of uses are scarce, because of rootstock/ scion graft incompatibilities that prevent the establishment of a strong and lasting functional union. Graft incompatibility is a problem in cherry, almond, and apricot than in peach or plum. In general, closely related cultivars and species tend to be compatible, but taxonomically distant plants often manifest incompatibility. This review will focus on the knowledge currently available on the metabolic response during the formation and establishment of the stock/scion graft union in order to help the effort for identify future metabolic markers to be used in breeding programs. The physiological, metabolic and molecular mechanisms that cause incompatibility remain unclear and several hypotheses have been proposed to explain it, mostly based on herbaceous species. Few studies are available to explain incompatibility in woody plants. Various phenolic compounds are known to affect cell division, development and differentiation at the graft union. Flavonol (catechins and proanthocyanidins concentrations increase shortly after grafting and, as a result of the stress induced during the healing response, vacuolar membrane disruption occurs resulting in the escape of phenols from the vacuole into the cytoplasmic matrix, causing dysfunctions in the growth of certain tissues (xylem and phloem, interference with the synthesis of lignin or inducing hormonal imbalances. All these abnormalities result in mechanical weakening of the union, which may manifest during the first year after grafting (translocated incompatibility or may appear several years later (localized incompatibility, leading to major

  2. The incompatible interaction between Phytophthora parasitica var. nicotianae race 0 and tobacco is suppressed in transgenic plants expressing antisense lipoxygenase sequences

    Rancé, Iann; Fournier, Joëlle; Esquerré-Tugayé, Marie-Thérèse

    1998-01-01

    Nicotiana tabacum 46-8 cultivar displays an incompatible interaction with race 0 of Phytophthora parasitica var. nicotianae (Ppn), a fungal pathogen of most tobacco cultivars. At the plant level, incompatibility is characterized by the induction of lipoxygenase (LOX, EC = 1.13.11.12) activity and localized hypersensitive cell death before defense gene activation. To evaluate the involvement of LOX in the onset of plant defense, tobacco 46-8 plants were genetically engineered using full-length...

  3. Comparative transcriptome analysis of stylar canal cells identifies novel candidate genes implicated in the self-incompatibility response of Citrus clementina

    Caruso Marco; Merelo Paz; Distefano Gaetano; La Malfa Stefano; Lo Piero Angela; Tadeo Francisco R; Talon Manuel; Gentile Alessandra

    2012-01-01

    Abstract Background Reproductive biology in citrus is still poorly understood. Although in recent years several efforts have been made to study pollen-pistil interaction and self-incompatibility, little information is available about the molecular mechanisms regulating these processes. Here we report the identification of candidate genes involved in pollen-pistil interaction and self-incompatibility in clementine (Citrus clementina Hort. ex Tan.). These genes have been identified comparing th...

  4. S-LOCUS EARLY FLOWERING 3 Is Exclusively Present in the Genomes of Short-Styled Buckwheat Plants that Exhibit Heteromorphic Self-Incompatibility

    Yasuo Yasui; Masashi Mori; Jotaro Aii; Tomoko Abe; Daiki Matsumoto; Shingo Sato; Yoriko Hayashi; Ohmi Ohnishi; Tatsuya Ota

    2012-01-01

    The different forms of flowers in a species have attracted the attention of many evolutionary biologists, including Charles Darwin. In Fagopyrum esculentum (common buckwheat), the occurrence of dimorphic flowers, namely short-styled and long-styled flowers, is associated with a type of self-incompatibility (SI) called heteromorphic SI. The floral morphology and intra-morph incompatibility are both determined by a single genetic locus named the S-locus. Plants with short-styled flowers are het...

  5. S-LOCUS EARLY FLOWERING 3 Is Exclusively Present in the Genomes of Short-Styled Buckwheat Plants that Exhibit Heteromorphic Self-Incompatibility.

    Yasui, Yasuo; Mori, Masashi; Aii, Jotaro; Abe, Tomoko; Matsumoto, Daiki; Sato, Shingo; Hayashi, Yoriko; Ohnishi, Ohmi; Ota, Tatsuya

    2012-01-01

    The different forms of flowers in a species have attracted the attention of many evolutionary biologists, including Charles Darwin. In Fagopyrum esculentum (common buckwheat), the occurrence of dimorphic flowers, namely short-styled and long-styled flowers, is associated with a type of self-incompatibility (SI) called heteromorphic SI. The floral morphology and intra-morph incompatibility are both determined by a single genetic locus named the S-locus. Plants with short-styled flowers are het...

  6. On the incompatibilities of interaction scales and processes with focus on the work of adhesion.

    Rosenholm, Jarl B

    2016-08-01

    The mutual compatibility of Hamaker constants, solubility parameters or cohesive energy densities (CED) and surface/interface tensions are evaluated. It is shown that the partial contributions (dispersive, Lifshitz-van der Waals, dipolar induction, dipolar orientation, polar, acid, base and hydrogen bond) to Hamaker constants, solubility parameters or cohesive energy densities and surface/interface tensions are mutually inconsistent. The published reference data for a single set of liquids is moreover shown to be exceedingly scattered; making the parallel use of these scales challenging. Reference processes designed for bringing two and three phases into mutual contact are conflicting. The two-phase processes within Hamaker and exchange energy density (EED) frameworks agree, but the three-phase models differ. As a free-standing parameter the EED is however comparable. The two-phase adhesion process is shown to be incompatible with the other contact processes and the three-phase adhesion process is opposite to them. One reason for this controversy is the different averaging of interfacial properties. While interfacial Hamaker constants and solubility parameters or cohesive energy densities are geometric averages of corresponding intervening phase properties, this practice is replaced by the work of adhesion being geometrically averaged as works of cohesion. As a result, there exist three conflicting models for the adhesion process: the Dupré work of adhesion, the Girifalco-Good geometric averaged works of cohesion and Fowkes reduced interfacial or interphasial tension process. None of these agree with the commonly accepted standard Hamaker contact processes and they should be replaced with the compatible extended work of adhesion process originally suggested by Dupré. The models offered for the conversion of Hamaker constants and solubility parameters or cohesive energy densities to surface tensions involve conversion factors and equilibrium distances between

  7. Case report: diffuse splenic metastasis of occult breast cancer with incompatible blood group antigenic determinants.

    Baranyay, Ferenc

    2009-01-01

    Cancer cells with immunogenic properties having altered protein glycosilation, modified blood group substances have been widely studied [Kannagi R, Miyake M, Zenita KM, Itai S, Hiraiwa N, Shigeta K, et al. Cancer-associated carbohydrate antigens: modified blood group substances and oncodevelopmental antigens on tumor cells. Gann Monogr Cancer Res 1988; 34: p. 15-28; Hakomori S. Antigen structure and genetic basis of histo-blood groups A, B and O their changes associated with human cancer. Biochem Biophys Acta 1999; 1473: p. 247-266; Brooks SA, Carter TM, Royle L, Harvey DJ, Fry SA, Kinch C, et al. Altered glycosilation of proteins in cancer: what is the potential for new anti-tumour strategies. Anticancer Agents Med Chem 2008; 8: p. 2-21]. In the study reported here, a 78-year-old female patient was admitted to the hospital with circulatory failure. At autopsy, the spleen (weight: 420 g) was extremely firm with a diffusely blackberry-colored cut surface. There were no signs of carcinomatous process at autopsy. By histology, the spleen showed diffuse metastatic carcinomatous infiltration. Using immunohistochemistry, an antibody to breast carcinoma antigen (BioGenex) labelled metastatic cells of the spleen and bone marrow. The patient was blood group O. Labelling for binding of lectins with and without blood group antigen specificity and monoclonal antibodies was carried out. The B blood group specific Banderiaea simplicifolia agglutinin I and an anti-B blood group monoclonal antibody labelled all the metastatic cells of spleen and bone marrow intensely. There was no detection of blood group A antigen by either binding of Dolichos biflorus agglutinin or anti-blood group A monoclonal antibodies. These observations raise the possibility that the detected incompatible B blood group antigen determinants on the metastatic cells were immunogenic. The surviving carcinoma cells may have found a place of refuge from immune surveillance in the spleen and in the bone marrow

  8. PK7300全自动血型分析仪测定ABO血型抗体效价的初步研究%A preliminary study on ABO blood type antibody titration by PK7300 automated blood typing analyzer

    石绍川; 刘东; 李军; 田耘博; 陈鹏

    2014-01-01

    目的:探索使用PK7300全自动血型分析仪测定ABO血型抗体效价的方法,探讨献血者血型检测中与ABO血型抗体效价相关的影响因素及注意事项。方法仪器微板法测定抽样标本的ABO血型抗体效价;仪器微板法及手工试管法测定各血型混合血浆的ABO血型抗体效价;统计分析该中心2013年5~7月检测出的ABO血型抗体效价降低的情况。结果A型献血者与B型献血者的ABO血型抗体效价比较,差异无统计学意义(P>0.05),O型献血者的ABO血型抗体效价明显高于A型或B型献血者(P<0.05);手工试管法的灵敏度明显优于仪器微板法,而手工试管法二的灵敏度又明显优于手工试管法一(P<0.05);ABO血型抗体效价降低的A型献血者明显多于B型或O型(均P<0.01),ABO血型抗体效价降低的男性献血者明显多于女性(P<0.01)。结论仪器微板法测定ABO血型抗体效价操作简便、结果判读客观、重复性好,1∶1~1∶80的稀释比例范围能满足绝大多数标本的测定需要,采用梯度稀释有助于减小测定误差;血型、性别、年龄、亚型等因素可能与献血者ABO血型抗体效价降低相关。%Objective To explore the detection method of ABO blood type antibody titration by the PK7300 automated blood typing analyzer and to investigate the ABO blood type antibody titer‐related influencing factors and the matter needing attentions in blood typing test for blood donors .Methods The ABO blood type antibody titers of the samples were measured by the analyzer microplate method;the ABO blood type antibody titer of each mixed plas‐ma was respectively measured by the analyzer microplate method and by the manual tube method;the decrease situa‐tion of ABO blood type antibody titers detected in our center from May to July 2013 were statistically analyzed .Re‐sults There was no statistically significant difference in the ABO

  9. Different Types of the Coagulation Disorders in Hamadan and A Comparison of the ABO & Rh Blood Group Distribution in the Patients and the Control Group

    H. Pour-Jafari

    2003-10-01

    Full Text Available Patients with hemophilia and thalassemia are two groups of hereditary disorders that, periodically, use blood and blood substances. The aims of the present study are determination of the frequencies of different types of coagulation disorders, and a comparison of the frequencies of ABO & Rh phenotypes among affected individuals with the control group (blood donors. The subject group were all affected persons were referred to hemophilia and thalassemia centers in Hamadan. Primary information was collected from their files, then were classified and analyzed. The control group were blood donors that were registered in Hamadan blood bank. Results showed that the different types of coagulation disorders in affected individuals referred to the Hemophilia and thalassemia centers during 1998 were Hemophilia A (61.96%, Hemophilia B (20.86%, Von Willbrand (5.52%, Platelet deficiency (4.91%, Factor XIII deficiency (3.68% and Factor VII deficiency (3.07%. In general, results showed that in population with Hemophilia A, frequencies of A and O blood types were statistically different with the blood donors. The lowest rate of negative Rh, was in the population with thalassemia. The sex ratio of the affected persons was also different with control group.

  10. Seroprevalence of Helicobacter pylori in dyspeptic patients and its relationship with HIV infection, ABO blood groups and life style in a university hospital, Northwest Ethiopia

    Feleke Moges; Afework Kassu; Getahun Mengistu; Solomon Adugna; Berhanu Andualem; Takeshi Nishikawa; Fusao Ota

    2006-01-01

    AIM: To determine the prevalence of Helicobacter pylori (H pylori) among dyspeptic patients and to assess the relationship between H pylori infection, blood group, HIV infection and life style of the patients.METHODS: In a hospital-based cross-sectional study,patients attending Outpatient Department of University of Gondar Hospital were enrolled. Socio-demographic information was collected using questionnaires. Serum was analyzed for anti-H pylori IgG antibodies using a commercial kit. HIV serostatus was determined by enzyme-linked immunosorbent assay (ELISA). Blood grouping was performed by slide agglutination tests.RESULTS: A total of 215 dyspeptic patients were included in the study. One hundred and sixteen patients (54%) were females and 99 (46%) were males. Anti-H pylori IgG antibodies were detected in sera of 184 (85.6%) patients. The prevalence was significantly higher in patients aged 50 years and above. Twenty point five percent of the patients were found to be seropositive for HIV. No significant association was found between sex,ABO blood groups, consumption of spicy diets, socioeconomic status and seropositivity for H pylori. However,alcohol consumption was significantly associated with H pylori serology.CONCLUSION: The prevalence of H pylori infection is associated with a history of alcohol intake and older age.The effect of different diet, alcohol and socioeconomic status as risk factors for H pylori infection needs further study.

  11. HIPOTIROIDISMO CANINO, PRESENTACION DE UN CASO ATIPICO

    Juan Carlos Ballut P; Marcelo Mieres L.

    2004-01-01

    Este trabajo describe las características clínicas de un caso de hipotiroidismo atípico, catalogado como de presentaciónoculta, caracterizado por signos relacionados con miopatía, neuropatía, deterioro del estado mental, sangrado

  12. HIPOTIROIDISMO CANINO, PRESENTACION DE UN CASO ATIPICO

    Juan Carlos Ballut P

    2004-12-01

    Full Text Available Este trabajo describe las características clínicas de un caso de hipotiroidismo atípico, catalogado como de presentaciónoculta, caracterizado por signos relacionados con miopatía, neuropatía, deterioro del estado mental, sangrado

  13. Incompatibilidad gametofítica en las razas mexicanas de maíz Gametophytic incompatibility in Mexican maize breeds

    José Miguel Padilla García

    2012-06-01

    Full Text Available La incompatibilidad esporofítica y gametofítica evitan la autofecundación o la fecundación cruzada, y es una forma de aislamiento reproductivo. Los genes de incompatibilidad-cruzada en maíz de los loci ga1 y tcb1, son causantes del reconocimiento o rechazo entre el estigma y el polen, y de ellos depende la presencia o ausencia de grano en la mazorca del maíz. El presente estudio tuvo como objetivo detectar alelos de incompatibilidad-cruzada de ga1 y tcb1 en colecciones representativas de grupos raciales de maíz de México. El trabajo se realizó en el Centro Universitario de Ciencias Biológicas y Agropecuarias de la Universidad de Guadalajara, ubicado en Zapopan, Jalisco, México durante los ciclos agrícolas de primavera-verano 2007 a 2009. Se evaluaron, en condiciones de temporal, 184 accesiones de razas mexicanas de maíz con base en porcentaje de llenado de grano de mazorca en sus cruzas con probadores macho recesivos (sin factores de incompatibilidad y probadores hembra dominantes (con factores de incompatibilidad. En el análisis de varianza hubo diferencias altamente significativas tanto entre razas como entre accesiones dentro de razas para el locus ga1. Con relación al locus tcb1 sólo se encontraron diferencias altamente significativas entre razas. El alelo Ga1-S, responsable de la incompatibilidad no-recíproca, se detectó 53.8% de las accesiones, mientras que el alelo neutro Ga1-m y el alelo recesivo ga1 se identificaron 55.43 y 15.76%, respectivamente. El alelo Tcb1-S fue detectado en muy bajas proporciones, concentrado principalmente en las razas de Elotes-Occidentales y Maíz-Dulce.Sporophytic and gametophytic incompatibility avoids self-fecundation or cross-fertilization, and it is a form of reproductive isolation. The cross-incompatibility genes in maize from loci ga1 and tcb1, cause recognition or reject between stigma and pollen, and from them depends presence or absence of grain in maize ear. The aim of this study

  14. CASO AlbAm S.A.

    Ricardo Goulart Serra

    2015-12-01

    Full Text Available RESUMO Os analistas financeiros da AlbAm, uma empresa do setor de autopeças, devem avaliar duas opções de aquisições (Cardblue e Garciadores. O objetivo do caso é servir de oportunidade para os alunos vivenciarem uma decisão de investimentos por meio do lucro econômico. Uma análise superficial certamente levará a decisão equivocada. Levando-se todos os aspectos do caso em consideração, chegar-se-á a uma solução que pode surpreender alguns alunos, uma vez que a empresa melhor gerida não será a melhor oportunidade de investimento. Também se pode aproveitar o caso para discutir a superficialidade da adoção do EBITDA como métrica de gestão comparativamente a uma métrica de valor agregado (lucro econômico - a melhor solução também não é aquela que gera o maior aumento em EBITDA. O caso pode ser usado em disciplinas de finanças (finanças corporativas ou avaliação de empresas em cursos de graduação, pós-graduação ou especialização. Todas as empresas e pessoas relatadas neste caso são fictícias.

  15. In vivo studies of the long-term 51Cr red cell survival of serologically incompatible red cell units

    The long-term survival of serologically incompatible red cell units was measured in five patients with antibodies to high-frequency antigens. Initially, the survival of 1 ml of 51Cr-labeled incompatible red cells was measured over 1 hour. After demonstrating that the 1-hour survival times were successful (greater than 70%), each patient then received 5 ml of the same 51Cr-labeled red cells followed by the transfusion of the remainder of the red cell unit. The long-term T 1/2Cr survival for each case was patient 1 (anti-McCa), 15 days; patient 2 (anti-JMH), 12 days; patient 3 (anti-Kna), 31 days; patient 4 (anti-McCa), 12 days; and patient 5 (anti-Hya), 14 days. Each antibody tested in an in vitro homologous macrophage assay showed less than 5 percent phagocytosis. Anti-JMH was the only antibody to react with IgG subclass antisera and was determined to be IgG4. The macrophage assay, IgG subclass testing, and short-term (1 hour, 1 ml) 51Cr survival studies all indicated that the short-term survival was good. However, only the measurement of long-term survival with transfused units of serologically incompatible red cells was able to determine the actual survival, and clinical significance of the alloantibodies. Determining the actual long-term survival by the method described here can be of importance for patients requiring chronic red cell transfusion

  16. Different levels of hyphal self-incompatibility modulate interconnectedness of mycorrhizal networks in three arbuscular mycorrhizal fungi within the Glomeraceae.

    Pepe, Alessandra; Giovannetti, Manuela; Sbrana, Cristiana

    2016-05-01

    Arbuscular mycorrhizal fungi (AMF) live in symbiosis with most plant species and produce underground extraradical hyphal networks functional in the uptake and translocation of mineral nutrients from the soil to host plants. This work investigated whether fungal genotype can affect patterns of interconnections and structural traits of extraradical mycelium (ERM), by comparing three Glomeraceae species growing in symbiosis with five plant hosts. An isolate of Funneliformis coronatus consistently showed low ability to form interconnected ERM and self-incompatibility that represented up to 21 % of hyphal contacts. The frequency of post-fusion self-incompatible interactions, never detected before in AMF extraradical networks, was 8.9 %. In F. coronatus ERM, the percentage of hyphal contacts leading to perfect hyphal fusions was 1.2-7.7, while it ranged from 25.8-48 to 35.6-53.6 in Rhizophagus intraradices and Funneliformis mosseae, respectively. Low interconnectedness of F. coronatus ERM resulted also from a very high number of non-interacting contacts (83.2 %). Such findings show that AMF genotypes in Glomeraceae can differ significantly in anastomosis behaviour and that ERM interconnectedness is modulated by the fungal symbiont, as F. coronatus consistently formed poorly interconnected networks when growing in symbiosis with five different host plants and in the asymbiotic stage. Structural traits, such as extent, density and hyphal self-compatibility/incompatibility, may represent key factors for the differential performance of AMF, by affecting fungal absorbing surface and foraging ability and thus nutrient flow from soil to host roots. PMID:26630971

  17. Wolbachia density and cytoplasmic incompatibility in Aedes albopictus: concerns with using artificial Wolbachia infection as a vector suppression tool.

    Maurizio Calvitti

    Full Text Available The mosquito Aedes albopictusi is a competent vector of harmful human pathogens, including viruses causing dengue and chikungunya. Cytoplasmic incompatibility (CI induced by endosymbiotic Wolbachia can be used to produce functionally sterile males that can be released in the field as a suppression tool against this mosquito. Because the available sexing methods are not efficient enough to avoid unintentional release of a few transinfected females, we assessed the CI pattern in crosses between wPip Wolbachia-transinfected (ARwP females and wild-type males of Ae. albopictus in this study. Quantitative polymerase chain reaction was used to monitor the titer of the Wolbachia strains that naturally infect Ae. albopictus, that is, wAlbA and wAlbB, in age-controlled males and females. Data were coupled with incompatibility level detected when the above-mentioned males were crossed with ARwP females. Wolbachia infection titer was also monitored in samples of wild caught males. Incompatibility level was positively correlated only with wAlbA density. Crosses between wild-type males having very low wAlbA density (<0.001 wAlbA/actin copy numbers and ARwP females were partially fertile (CIcorr = 68.06 ± 6.20. Individuals with low wAlbA titer were frequently found among sampled wild males (30%-50% depending on the site and period. ARwP males can be as considered as a very promising tool for suppressing Ae. albopictus. However, crosses between wild males having low wAlbA density and ARwP females may be partially fertile. In the case of local establishment of the transinfected mosquito line, this occurrence may favor the replacement of the wild-type mosquitoes with the ARwP line, thus reducing the long-term efficacy of incompatible insect technique. Various alternative strategies have been discussed to prevent this risk and to exploit Wolbachia as a tool to control Ae. albopictus.

  18. Molecular characterization of five new S alleles associated with self-incompatibility in local Spanish almond cultivars

    Kodad, Ossama; Sánchez, A.; Saibo, N.; M. M. Oliveira; Socias i Company, Rafel

    2011-01-01

    Almond is a highly heterozygous species with a high number of S-alleles controlling its gametophytic self-incompatibility system (GSI). In this work we have analysed Spanish local almond cultivars for S-RNase allele diversity. By cloning and sequencing five new S-RNase alleles were identified: S31 (804 bp) in 'Pou de Felanitx' and 'Totsol', S32 (855 bp) in 'Taiatona', S33 (1165 bp) in 'Pou d'Establiments' and 'Muel', S34 (1663 bp) in 'Pané-Barquets', and S35 (1658 bp) in 'Planeta de les Garri...

  19. Reproductive Incompatibility Involving Senegalese Aedes aegypti (L) Is Associated with Chromosome Rearrangements

    Dickson, Laura B.; Sharakhova, Maria V.; Timoshevskiy, Vladimir A.; Fleming, Karen L.; Caspary, Alex; Sylla, Massamba; Black, William C.

    2016-01-01

    was used to identify AT-rich regions, chromomycin A3 following pretreatment with barium hydroxide stained for GC-rich regions and stained the ribosomal RNA locus and YOYO-1 was used to test for differential staining. Chromosome patterns in SenAae strains revealed by these three stains differed from those in IB12. For FISH, 40 BAC clones previously physically mapped on Aaa chromosomes were used to test for chromosome rearrangements in SenAae relative to IB12. Differences in the order of markers identified two chromosomal rearrangements between IB12 and SenAae strains. The first rearrangement involves two overlapping pericentric (containing the centromere) inversions in chromosome 3 or an insertion of a large fragment into the 3q arm. The second rearrangement is close to the centromere on the p arm of chromosome 2. Linkage analysis of the SDL and the white-eye locus identified a likely chromosomal rearrangement on chromosome 1. The reproductive incompatibility observed within SenAae and between SenAae and Aaa may be generally associated with chromosome rearrangements on all three chromosomes and specifically caused by pericentric inversions on chromosomes 2 and 3. PMID:27105225

  20. Reproductive Incompatibility Involving Senegalese Aedes aegypti (L) Is Associated with Chromosome Rearrangements.

    Dickson, Laura B; Sharakhova, Maria V; Timoshevskiy, Vladimir A; Fleming, Karen L; Caspary, Alex; Sylla, Massamba; Black, William C

    2016-04-01

    used to identify AT-rich regions, chromomycin A3 following pretreatment with barium hydroxide stained for GC-rich regions and stained the ribosomal RNA locus and YOYO-1 was used to test for differential staining. Chromosome patterns in SenAae strains revealed by these three stains differed from those in IB12. For FISH, 40 BAC clones previously physically mapped on Aaa chromosomes were used to test for chromosome rearrangements in SenAae relative to IB12. Differences in the order of markers identified two chromosomal rearrangements between IB12 and SenAae strains. The first rearrangement involves two overlapping pericentric (containing the centromere) inversions in chromosome 3 or an insertion of a large fragment into the 3q arm. The second rearrangement is close to the centromere on the p arm of chromosome 2. Linkage analysis of the SDL and the white-eye locus identified a likely chromosomal rearrangement on chromosome 1. The reproductive incompatibility observed within SenAae and between SenAae and Aaa may be generally associated with chromosome rearrangements on all three chromosomes and specifically caused by pericentric inversions on chromosomes 2 and 3. PMID:27105225

  1. Women’s rights: from bad to worse? Assessing the evolution of incompatible reservations to the CEDAW Convention

    Marijke de Pauw

    2013-07-01

    Full Text Available The Convention on the Elimination of All Forms of Discrimination against Women is the most important international human rights instrument for the protection of women's rights worldwide. It is, however, also one of the main UN human rights treaties to which the largest number of reservations has been made. As far-reaching reservations to human rights treaties are detrimental to the effective protection of human rights, this gave rise to the debate on the need to apply a reservations system to such treaties that allows for the efficient protection of their integrity. More specifically, it resulted in the severability approach and treaty bodies claiming their competence to assess reservations' compatibility. This article aims to contribute to the academic research on reservations to the CEDAW Convention, by studying the evolution of reservation-making to this human rights treaty from a comparative perspective from its entry into force until the present. Through this analysis, the article aims to identify trends and shifts in this practice over time, allowing for a more detailed assessment of the increasing number of incompatible reservations, the Committee's progress towards a more active approach on reservations, and State's increasing willingness to object to reservations they find incompatible.

  2. BARTERING FOR A COMPATIBLE KIDNEY USING YOUR INCOMPATIBLE, LIVE KIDNEY DONOR: LEGAL AND ETHICAL ISSUES RELATED TO KIDNEY CHAINS.

    Tenenbaum, Evelyn M

    2016-01-01

    Kidney chains are a recent and novel method of increasing the number of available kidneys for transplantation and have the potential to save thousands of lives. However, because they are novel, kidney chains do not fit neatly within existing legal and ethicalframeworks, raising potential barriers to their full implementation. Kidney chains are an extension of paired kidney donation, which began in the United States in 2000. Paired kidney donations allow kidney patients with willing, but incompatible, donors to swap donors to increase the number of donor/recipient pairs and consequently, the number of transplants. More recently, transplant centers have been using non-simultaneous, extended, altruistic donor ("NEAD") kidney chains--which consist of a sequence of donations by incompatible donors--to further expand the number of donations. This Article fully explains paired kidney donation and kidney chains and focuses on whether NEAD chains are more coercive than traditional kidney donation to a family member or close friend and whether NEAD chains violate the National Organ Transplant Act's prohibition on the transfer of organs for valuable consideration. PMID:27263265

  3. The influence of incompatibility and dielectric contrast on the electric field-induced orientation of lamellar block copolymers

    We investigate the influence of incompatibility and dielectric contrast on the reorientation kinetics of concentrated solutions of lamellar block copolymers in the presence of an external DC electric field. We study solutions of AC diblock copolymer and ABC triblock terpolymers. The inclusion of a short, polar middle block B is used to tailor both the degree of incompatibility and the dielectric contrast between the two majority phases. In situ synchrotron radiation small-angle X-ray scattering is used to monitor the reorientation process. For the AC diblock copolymer sample only weak electric field induced reorientation could be achieved, following a very slow kinetics, whereas for the ABC triblock terpolymer, reorientation is observed above a threshold value of 0.3 kV/mm. The orientation kinetics is well described by a single exponential with characteristic time constants varying between a few seconds and several minutes depending on the polymer concentration and the electric field strength. We identify a narrow concentration window, in which the interplay between chain mobility and gain in free energy in the electric field allows the preparation of highly anisotropic bulk polymer samples by exposure to an electric field. The results are compared to free energy calculations revealing a distinct difference in the driving force for reorientation as a consequence of an increase in dielectric contrast and chain mobility upon introduction of the B middle block.

  4. The population genetics of sporophytic self-incompatibility in three hybridizing senecio (asteraceae) species with contrasting population histories.

    Brennan, Adrian C; Harris, Stephen A; Hiscock, Simon J

    2013-05-01

    Hybridization generates evolutionary novelty and spreads adaptive variation. By promoting outcrossing, plant self-incompatibility (SI) systems also favor interspecific hybridization because the S locus is under strong negative frequency-dependent balancing selection. This study investigates the SI mating systems of three hybridizing Senecio species with contrasting population histories. Senecio aethnensis and S. chrysanthemifolius native to Sicily, form a hybrid zone at intermediate altitudes on Mount Etna, and their neo-homoploid hybrid species, S. squalidus, has colonized disturbed urban habitats in the UK during the last 150 years. We show that all three species express sporophytic SI (SSI), where pollen incompatibility is controlled by the diploid parental genome, and that SSI is inherited and functions normally in hybrids. Large-scale crossing studies of wild sampled populations allowed direct comparison of SSI between species and found that the main impacts of colonization in S. squalidus compared to Sicilian Senecio was a reduced number of S alleles, increased S allele frequencies, and increased interpopulation S allele sharing. In general, many S alleles were shared between species and the S locus showed reduced intra- and interspecific population genetic structure compared to molecular genetic markers, indicative of enhanced effective gene flow due to balancing selection. PMID:23617913

  5. Incest versus abstinence: reproductive trade-offs between mate limitation and progeny fitness in a self-incompatible invasive plant.

    Pierson, Jennifer C; Swain, Stephen M; Young, Andrew G

    2013-12-01

    Plant mating systems represent an evolutionary and ecological trade-off between reproductive assurance through selfing and maximizing progeny fitness through outbreeding. However, many plants with sporophytic self-incompatibility systems exhibit dominance interactions at the S-locus that allow biparental inbreeding, thereby facilitating mating between individuals that share alleles at the S-locus. We investigated this trade-off by estimating mate availability and biparental inbreeding depression in wild radish from five different populations across Australia. We found dominance interactions among S-alleles increased mate availability relative to estimates based on individuals that did not share S-alleles. Twelve of the sixteen fitness variables were significantly reduced by inbreeding. For all the three life-history phases evaluated, self-fertilized offspring suffered a greater than 50% reduction in fitness, while full-sib and half-sib offspring suffered a less than 50% reduction in fitness. Theory indicates that fitness costs greater than 50% can result in an evolutionary trajectory toward a stable state of self-incompatibility (SI). This study suggests that dominance interactions at the S-locus provide a possible third stable state between SI and SC where biparental inbreeding increases mate availability with relatively minor fitness costs. This strategy allows weeds to establish in new environments while maintaining a functional SI system. PMID:24455137

  6. PollenCALC: Software for estimation of pollen compatibility of self-incompatible allo- and autotetraploid species

    Aguirre Andrea

    2012-06-01

    Full Text Available Abstract Background Self-incompatibility (SI is a biological mechanism to avoid inbreeding in allogamous plants. In grasses, this mechanism is controlled by a two-locus system (S-Z. Calculation of male and female gamete frequencies is complex for tetraploid species. We are not aware of any software available for predicting pollen haplotype frequencies and pollen compatibility in tetraploid species. Results PollenCALC is a software tool written in C++ programming language that can predict pollen compatibility percentages for polyploid species with a two-locus (S, Z self-incompatibility system. The program predicts pollen genotypes and frequencies based on defined meiotic parameters for allo- or autotetraploid species with a gametophytic S-Z SI system. These predictions can be used to obtain expected values for for diploid and for (allo- or autotetraploidy SI grasses. Conclusion The information provided by this calculator can be used to predict compatibility of pair-crosses in plant breeding applications, to analyze segregation distortion for S and Z genes, as well as linked markers in mapping populations, hypothesis testing of the number of S and Z alleles in a pair cross, and the underlying genetic model.

  7. ABO血型与男性精液参数的关系研究%Study on the relationship between ABO blood types and male semen parameters

    张欣宗; 吴红军; 姚康寿

    2011-01-01

    Objective To investigate the relationship between ABO blood types and male semen parameters.Methods From Sep of 2007 to Nov of 2009, 1793 semen samples collected from 1793 donors of Zhejiang Human Sperm Bank were divided into blood type A group(537), blood type B group(518), blood type O group(586) and blood type AB group(152) based on donors' blood type. Sperm morphology and semen characteristics were assessed according to the World Health Organization criteria. Results The total motility of sperm in blood type A group(53.32%±l5.15%)was significantly lower than that in blood type B group and blood type O group(55.17%±15.13%, 55.89%±14.33%)(P <0.05), and slightly lower than that in blood type AB group(54.22%±12.88%)(P>0.05).No significant differences in semen volume(ml), sperm concentration (106 per ml) and sperm morphology (normal forms, %) were found among the four groups (P>0.05). Conclusion There were no differences in semen volume(ml), sperm concentration (106 per ml) and sperm morphology (normal forms, %) among different ABO blood donors. But the total motility of sperm of blood type A donors was lower than that of other blood type donors, which might be related to the characteristics of blood type A donors such as introvert, depression and extreme behavior.%目的 了解ABO血型与男性精液参数的关系.方法 1793例研究对象均来自浙江省人类精子库2007年9月至2009年11月的供精志愿者.根据ABO血型分组,其中A型537例,B型518例,O型586例,AB型152例.所有供精者均留取精液标本进行精液常规分析及精子形态学分析.结果 四组之间精液量、精子浓度、形态正常精子百分率等参数差异均无统计学意义;但A型供精者的精子活动率(53.32±15.15)%明显低于B型的(55.17±15.13)%及0型供精者(55.89±14.33)%,差异有统计学意义(P0.05).结论 不同血型男性的精液量、精子浓度、形态正常精子百分率没有差别,但A型血男性

  8. Comparative Study of Nei�s D with other Genetic Distance Measures between Barak Valley Muslims and other Nations for ABO Locus

    Supriyo CHAKRABORTY

    2012-02-01

    Full Text Available Quantification of the genetic distance between populations is essential in many genetic research programs. Several formulae were proposed for the estimation of genetic distance between populations using gene frequency data. But the selection of a suitable measure for estimating genetic distance between real-world human populations is a very difficult task despite the widely used measure Nei�s D. The present study was undertaken to estimate the genetic distance between Barak Valley Muslims (BVM and other twenty-four nations using seven different measures with ABO blood group gene frequency data for comparative analysis and to estimate the correlation coefficients between distance measures and to work out the linear regression equations. Seven genetic distance measures namely Nei�s D, Nei�s Nm, La, Nei�s Da, Dc, Re and Nei�s Ne were estimated between BVM and other 24 nations enroute the journey of mankind from Africa that commenced about 200,000 years ago (www.bradshawfoundation.com. Correlation coefficients between Nei�s D with other measures were estimated to find out which other genetic distance measures were closely related to Nei�s D. Nei�s D showed highly significant (p=0.01 positive correlation with Cavalli-Sforza and Edwards chord distance Dc (0.90, Reynolds Re (0.90, Nei�s Da (0.74 and Nei�s Ne (0.63 but negative correlation with Nei�s Nm and La. Linear regression equations of Nei�s D with other distance measures were estimated as Da = -0.80 + 1.34D, Dc = 1.91 + 4.44D, Re = -0.51 + 0.24D and Ne = -7.60 + 1.30D.

  9. The O-Linked Glycome and Blood Group Antigens ABO on Mucin-Type Glycoproteins in Mucinous and Serous Epithelial Ovarian Tumors.

    Varvara Vitiazeva

    Full Text Available Mucins are heavily O-glycosylated proteins where the glycosylation has been shown to play an important role in cancer. Normal epithelial ovarian cells do not express secreted mucins, but their abnormal expression has previously been described in epithelial ovarian cancer and may relate to tumor formation and progression. The cyst fluids were shown to be a rich source for acidic glycoproteins. The study of these proteins can potentially lead to the identification of more effective biomarkers for ovarian cancer.In this study, we analyzed the expression of the MUC5AC and the O-glycosylation of acidic glycoproteins secreted into ovarian cyst fluids. The samples were obtained from patients with serous and mucinous ovarian tumors of different stages (benign, borderline, malignant and grades. The O-linked oligosaccharides were released and analyzed by negative-ion graphitized carbon Liquid Chromatography (LC coupled to Electrospray Ionization tandem Mass Spectrometry (ESI-MSn. The LC-ESI-MSn of the oligosaccharides from ovarian cyst fluids displayed differences in expression of fucose containing structures such as blood group ABO antigens and Lewis-type epitopes.The obtained data showed that serous and mucinous benign adenomas, mucinous low malignant potential carcinomas (LMPs, borderline and mucinous low-grade carcinomas have a high level of blood groups and Lewis type epitopes. In contrast, this type of fucosylated structures were low abundant in the high-grade mucinous carcinomas or in serous carcinomas. In addition, the ovarian tumors that showed a high level of expression of blood group antigens also revealed a strong reactivity towards the MUC5AC antibody. To visualize the differences between serous and mucinous ovarian tumors based on the O-glycosylation, a hierarchical cluster analysis was performed using mass spectrometry average compositions (MSAC.Mucinous benign and LMPs along with mucinous low-grade carcinomas appear to be different from

  10. Identification of the het-r vegetative incompatibility gene of Podospora anserina as a member of the fast evolving HNWD gene family.

    Chevanne, Damien; Bastiaans, Eric; Debets, Alfons; Saupe, Sven J; Clavé, Corinne; Paoletti, Mathieu

    2009-02-01

    In fungi, vegetative incompatibility is a conspecific non-self recognition mechanism that restricts formation of viable heterokaryons when incompatible alleles of specific het loci interact. In Podospora anserina, three non-allelic incompatibility systems have been genetically defined involving interactions between het-c and het-d, het-c and het-e, het-r and het-v. het-d and het-e are paralogues belonging to the HNWD gene family that encode proteins of the STAND class. HET-D and HET-E proteins comprise an N-terminal HET effector domain, a central GTP binding site and a C-terminal WD repeat domain constituted of tandem repeats of highly conserved WD40 repeat units that define the specificity of alleles during incompatibility. The WD40 repeat units of the members of this HNWD family are undergoing concerted evolution. By combining genetic analysis and gain of function experiments, we demonstrate that an additional member of this family, HNWD2, corresponds to the het-r non-allelic incompatibility gene. As for het-d and het-e, allele specificity at the het-r locus is determined by the WD repeat domain. Natural isolates show allelic variation for het-r. PMID:19137300

  11. Detection of self-incompatible oilseed rape plants (Brassica napus L. based on molecular markers for identification of the class I S haplotype

    Lenka Havlícková

    2014-09-01

    Full Text Available The selection of desirable genotypes with recessive characteristics, such as self-incompatible plants, is often difficult or even impossible and represents a crucial barrier in accelerating the breeding process. Molecular approaches and selection based on molecular markers can allow breeders to overcome this limitation. The use of self-incompatibility is an alternative in hybrid breeding of oilseed rape. Unfortunately, stable self-incompatibility is recessive and phenotype-based selection is very difficult and time-consuming. The development of reliable molecular markers for detecting desirable plants with functional self-incompatible genes is of great importance for breeders and allows selection at early stages of plant growth. Because most of these reliable molecular markers are based on discrimination of class I S-locus genes that are present in self-compatible plants, there is a need to use an internal control in order to detect possible PCR inhibition that gives false results during genotyping. In this study, 269 double haploid F2 oilseed rape plants obtained by microspore embryogenesis were used to verify the applicability of an improved PCR assay based on the detection of the class I SLG gene along with an internal control. Comparative analysis of the PCR genotyping results vs. S phenotype analysis confirmed the applicability of this molecular approach in hybrid breeding programs. This approach allows accurate detection of self-incompatible plants via a different amplification profile.

  12. Dosimetric properties of CaSO4: Dy thermoluminescent material

    The new ICRP regulations about radiological protection allows the different groups to study new high sensitivity thermoluminescent materials for dosimetric applications (personal and environmental). Different concentrations of Dy as dopant in the thermoluminescent material CaSO4 have been studied. The concentration usually encountered in bibliography for the CaSO4 is 0.1%. Our group have chosen the 1% Dy concentration due to its high sensitivity, the ratio for the CaSO4: Dy (1%) over the CaSO4: Dy (0.1%) is close to 3. The results obtained for the habitual set of tests (homogeneity, reproducibility, detection threshold, energy response and fading) reveals CaSO4: Dy (1%) as an excellent dosimetric material. We also present the good behavior of the CaSO4: Dy (0.1%) as a material for a retrospective dosimetry. (Author)

  13. Paralisia periodica tireotoxica: registro de um caso

    Athos Scherer Torres

    1979-06-01

    Full Text Available É relatado um caso de paralisia periódica tireotóxica. Os autores chamam a atenção para o fato de não haver registro de publicações semelhantes na literatura brasileira nos 10 últimos anos. São abordados diversos aspectos do diagnóstico diferencial com outros tipos de paralisia periódica.

  14. Analysis on irregular antibody inducing unconformity of forward and reverse ABO blood typing%不规则抗体导致ABO正反定型不符处理探讨

    樊凤艳; 王洪波; 魏少平; 魏利召; 刘晓莉; 祁术元

    2012-01-01

    Objective To investigate the factors of inducing the unconformity of forward and reverse ABO blood typing caused by irregular antibody. Methods The results of serological tests of blood typing in 12 cases were analyzed, and the factors of irregular antibody inducing the unconformity of forward and reverse ABO blood typing were tested for irregular antibody screening and identification and corresponding blood typing determination. Reverse ABO blood typing were repeated with red blood cells which corresponding antigens were negative. Results The amounts of alloantibodies among 12 patients were 4 anti-M,l anti-N, 1 anti-M combined anti-N,l anti- Lea, 1 anti- P1 ,1 anti-I,1 anti-D, 1 anti-E and 1 anti-A1. The result of repeated reverse blood typing with corresponding antigens being negative blood cells matched forward blood typing. Conclusions Samples with unconformity of forward and reverse ABO blood typing caused by irregular antibody can be identified correctly.%目的 探讨不规则抗体导致ABO正反定型不符原因,并提出解决方法.方法 总结分析12例不规则抗体导致ABO正反定型不符血型血清学试验结果,通过不规则抗体筛查、不规则抗体鉴定、相应血型检测明确不规则抗体导致ABO正反定型不符确切原因,用相应抗原阴性红细胞重新进行ABO血型反定型.结果 12例标本中抗-M 4例,抗-N 1例,抗-M合并抗-N 1例,抗-Lea 1例,抗-P1 1例,抗-I 1例,抗-D 1例,抗-E 1例,抗-A1 1例.用相应抗原阴性红细胞重新进行ABO血型反定型,正反定型结果均相符.结论 不规则抗体是导致ABO正反定型不符的常见原因,经过适当处理可以正确鉴定ABO血型.

  15. Meta analysis of the effect of immunoglobulin infusion on neonatal isoimmune hemolytic disease caused by blood group incompatibility%新生儿血型不合溶血病丙种球蛋白治疗安全性和有效性的Meta分析

    李志华; 王瑾; 陈超

    2010-01-01

    immunoglobulin infusion on neonatal isoimmune hemolytic disease by meta analysis.Method All randomized controlled trials on the effect of immunoglobulin infusion on neonatal Rh and ABO incompatible hemolytic disease obtained by searching MEDLINE,Cochrane Library,EMBASE,CNKI and CBM were included.Meta analysis was done by Review Manager 4.2software.Results Six trials with totally 456 neonates were included.There were 109 infants with Rh blood group incompatible hemolysis in 4 studies and 347 infants with ABO blood group incompatible hemolysis in 4 studies.There was no significant difference in gestational age,weight and sex between the immunoglobulin infusion and control groups.Compared with those neonates treated with only phototherapy,the infants treated with immunoglobulin and phototherapy had shorter duration of phototherapy(weighted mean difference,WMD - 15.42,95% CI - 29.00 to - 1.85),less chance to be given exchange transfusion(RR 0.25,95% GI 0.17 to 0.39)and shorter duration of hospitalization(WMD - 25.44,95% GI - 36.93 to- 13.94).While intravenous immunoglobulin could not decrease the maximum serum bilirubin level(WMD -29.91,95%CI -78.24 to 18.42).There was no significant difference in the incidence of late anemiabetween the two groups.No adverse reaction was found in neonates who received immunoglobulin.Conclusions The results of this meta analysis support that the intravenous immunoglobulin had some therapeutic effect on neonatal isoimmune hemolytic disease.The infants who received immunoglobulin had shorter duration of phototherapy and less chance to be given exchange transfusion.Well designed,double blind and randomized controlled trials with large sample size and long-term follow-up are needed for further evaluation of the efficacy and safety of the immunoglobulin therapy.

  16. CARCINOMA HEPATOCELULAR CANINO: REPORTE DE UN CASO

    Edwin Buriticá G

    2009-08-01

    Full Text Available Objetivo Presentar el caso de un paciente canino, con enfermedad hepática, asociada a carcinoma hepatocelular. Materiales y métodos Fue estudiado el caso de un paciente canino de raza Pitt bull de 10 años de edad, al que se le encontraron múltiples nódulos distribuidos en todo el parénquima hepático, con diagnostico histopatológico de carcinoma hepatocelular. Resultados Se expone el caso clínico de un paciente canino de raza Pitt bull, que es presentado a consulta externa por presentar decaimiento y distensión abdominal, una vez realizado el examen físico sistemático se evidenció hepatomegalia y un crecimiento anormal hacia la región del mesogastrio derecho. Los exámenes paraclinicos e histopatológicos demostraron la presencia de enfermedad hepática asociada a carcinoma hepatocelular. Conclusiones El carcinoma hepatocelular es una entidad de rara presentación, clínicamente cursa con signos muy similares a otras alteraciones tumorales de compromiso hepático, su diagnostico suele ser histopatológico.

  17. Monocephalus dipygus EN CACHARROS: REPORTE DE DOS CASOS

    Teresa Oviedo S

    2008-12-01

    Full Text Available Se describen dos casos de duplicación posterior congénita en cachorros. Esta malformación también conocida como Monocephalus dipygus, hace referencia a individuos dobles, caracterizados por tener cabeza y tórax fusionados en uno solo, duplicidad simétrica de pelvis y miembros posteriores. El primer caso, presentó cuatro miembros anteriores, cuatro miembros posteriores y palatosquisis. El segundo caso, presentó dos miembros anteriores y cuatro miembros posteriores. En general, ambos casos pueden ser relacionados como gemelos fusionados simétricos.

  18. Rodilla flotante: Revisión retrospectiva de 24 casos

    Ruiz Arranz, José Luis; Queipo de LLano Temboury, Alfonso; Godino Izquierdo, M.

    2001-01-01

    Se realiza una evaluación retrospectiva de 24 pacientes tratados de fracturas ipsilaterales de fémur y tibia. Solo en 7 (29%) casos esta lesión no se acompañaba de otras lesiones asociadas. Los resultados se han evaluado según la escala modificada de Thorensen y cols. (1985). El tipo de fractura fue muy polimorfo. Se realizó tratamiento definitivo de ambos huesos en la urgencia en 5 casos, en 7 casos se hizo tratamiento definitivo en tibia y provisional del fémur, y en 12 casos el tratamiento...

  19. Actinomicosis abdominal. Presentación de tres casos

    Adolfo Olvera-Reynada; Marco Antonio Calzada-Ramos; Xavier Espinoza-Guerrero; Cuauhtémoc Molotla-Xolalpa; Pedro de Jesús Cervantes-Miramontes

    2005-01-01

    Objetivo: informar tres casos de actinomicosis abdominal, uno que se presenta como tumor abdominal y dos que se intervienen de urgencia por abdomen agudo con diagnóstico de apendicitis aguda complicada. Casos clínicos: Caso 1. Mujer de 36 años con masa abdominal en fosa iliaca izquierda dependiente de colon, observada en la tomografía axial computarizada de abdomen. Se llevó a cabo resección del tumor. Casos 2 y 3. Mujeres de 37 y 39 años, respectivamente, que acudieron a urgencias por dolor ...

  20. MIASIS CAVITARIA: REPORTE DE UN CASO MIASIS CAVITARIA: REPORTE DE CASO CAVITARY MYIASIS: CASE REPORT

    CESAR GONZÁLEZ A; JUAN CARLOS SALAMANCA G; VICTOR OLANO M; CARLOS E PÉREZ

    2008-01-01

    La miasis es una enfermedad producida en tejidos o en cavidades corporales por la infestación de larvas de algunas especies de dípteros; su clasificación se hace según el sitio anatómico de infestación o de acuerdo al ciclo vital de la especie. En Colombia, la mayoría de los casos reportados de miasis cavitaria son debidos a Cochiliomyia hominivorax. Se reporta el caso de una paciente desnutrida con perforación del septo nasal, a quien al realizarle endoscopia nasal se le extraen once larvas ...

  1. Efetividade da Terapia Manual Ortopédica num Caso de Cervicalgia – Estudo de caso

    Silva, Mafalda Carreteiro e

    2015-01-01

    Introdução: Existem diversas causas e/ou estruturas responsáveis pela dor cervical, pelo que o sucesso do tratamento depende do diagnóstico diferencial e da escolha do tratamento mais adequado a cada situação. Objetivo: Descrever um plano de intervenção em fisioterapia num caso clínico de cervicalgia com base na terapia manual e exercícios terapêuticos. Métodos: Este estudo de caso refere-se a uma utente do sexo feminino, 47 anos, que foi encaminhada aos serviços de fisioterapia após um acide...

  2. Mantle in the Manihiki Plateau source with ultra-depleted incompatible element abundances but FOZO-like isotopic signature

    Golowin, R.; Hoernle, K.; Portnyagin, M.; Hauff, F.; Gurenko, A.; Garbe-Schoenberg, C. D.; Werner, R.

    2014-12-01

    The ~120Ma Manihiki Plateau basement consists of high-Ti tholeiitic basalts with EM-I type isotopic signatures, similar to the Singgalo basalts at Ontong Java, and low-Ti tholeiitic basalts with FOZO (Kwaimbaita/Kroenke) to HIMU-type isotopic compositions, similar to late stage volcanism on Hikurangi and Manihiki Plateaus (Hoernle et al. 2010; Timm et al. 2011). The low-Ti basalts have affinities to boninites and have been interpreted to be derived from residual mantle wedge mantle (Ingle et al. 2007). New major, volatile and trace element and radiogenic isotope data have been generated from fresh low-Ti glass samples recovered during R/V Sonne cruises SO193 and SO225. The low-Ti samples have distinctly lower Ti/V ratios compared to lavas from Ontong Java Plateau (Kwaimbaita-Kroenke and Singgalo), but similar to boninitic rocks. Glasses and melt inclusions in olivine have low volatile contents (0.12-0.25 wt% H2O). Olivine chemistry points to derivation from peridotite source. Therefore we interpret the low-Ti lavas to have formed through melting of dry and depleted peridotite at high temperatures, consistent with Timm et al (2011). The low-Ti group is characterized by U-shaped trace element patterns. The glass samples form linear mixing arrays on radiogenic isotope diagrams, pointing to the involvement of two components: 1) a component ultra-depleted in highly incompatible elements (UDC) but with intermediate Pb, Sr and Nd isotopic compositions, being similar to Kwaimbaita/Kroenke lavas from Ontong Java, and 2) an enriched component with HIMU-type incompatible element and isotopic characteristics, similar to late-stage volcanism on Manihiki, Hikurangi and Ontong Java (e.g. Hoernle et al. 2010). The ultra-depleted, FOZO-like mantle component could represent second stage melting of FOZO type mantle or re-melting of young recycled oceanic lithosphere within the plume head. Enrichment with HIMU type melts is required to explain the enrichment in the most incompatible

  3. Incompatible Trace Elements in Olivine: Using Sc, Y and V as Temperature and Redox Monitors in Basaltic Magmas

    Mallmann, G.; O'Neill, H. S.

    2012-12-01

    Olivine is the dominant constituent phase of the Earth's upper mantle and the first silicate mineral to crystallize from primitive basaltic melts on cooling following decompression. The physical and chemical properties of olivine have, therefore, been of great interest to geochemists and geophysicists. Yet, olivine is so poor in incompatible trace elements (phosphorus being the exception) that it features in much geochemical modeling merely as an inert dilutant. Consequently, our understanding of incompatible trace-element partitioning between olivine and silicate melt has lagged behind that of phases such as pyroxenes or garnet, which control bulk crystal/melt partitioning behavior during mantle melting. Advances in trace-element microanalysis, particularly LA-ICP-MS, have now placed the determination of incompatible elements in natural olivines within reach, and recent studies have shown that mantle and magmatic olivines can preserve complex intracrystalline distributions of these elements. The combined major and trace element compositions of phenocrystal olivines could, therefore, provide unique and detailed insights into magmatic evolution. The course of evolution of basaltic magmas depends substantially on their redox state, hence oxygen fugacity, but there is increasing evidence that this intensive thermodynamic variable may be less well understood in basalts than commonly supposed. The redox state of terrestrial basalts has to a large extent been inferred from the Fe3+/Fe2+ ratios of their quenched glasses. However, this quantity appears to be significantly affected during late and post-eruptive processes in magmatic systems (e.g. by degassing, charge-transfer reactions of redox-variable species, and alteration), so that the degree to which the Fe3+/Fe2+ ratios preserved in basaltic glasses reflect the oxidation state of the magma at high temperature is unclear. The equilibrium partitioning relations preserved in olivine phenocrysts in basalts are, in

  4. Cytoplasmic incompatibility as a means of controlling Culex pipiens quinquefasciatus mosquito in the islands of the south-western Indian Ocean.

    Atyame, Célestine M; Pasteur, Nicole; Dumas, Emilie; Tortosa, Pablo; Tantely, Michaël Luciano; Pocquet, Nicolas; Licciardi, Séverine; Bheecarry, Ambicadutt; Zumbo, Betty; Weill, Mylène; Duron, Olivier

    2011-12-01

    The use of the bacterium Wolbachia is an attractive alternative method to control vector populations. In mosquitoes, as in members of the Culex pipiens complex, Wolbachia induces a form of embryonic lethality called cytoplasmic incompatibility, a sperm-egg incompatibility occurring when infected males mate either with uninfected females or with females infected with incompatible Wolbachia strain(s). Here we explore the feasibility of the Incompatible Insect Technique (IIT), a species-specific control approach in which field females are sterilized by inundative releases of incompatible males. We show that the Wolbachia wPip(Is) strain, naturally infecting Cx. p. pipiens mosquitoes from Turkey, is a good candidate to control Cx. p. quinquefasciatus populations on four islands of the south-western Indian Ocean (La Réunion, Mauritius, Grande Glorieuse and Mayotte). The wPip(Is) strain was introduced into the nuclear background of Cx. p. quinquefasciatus mosquitoes from La Réunion, leading to the LR[wPip(Is)] line. Total embryonic lethality was observed in crosses between LR[wPip(Is)] males and all tested field females from the four islands. Interestingly, most crosses involving LR[wPip(Is)] females and field males were also incompatible, which is expected to reduce the impact of any accidental release of LR[wPip(Is)] females. Cage experiments demonstrate that LR[wPip(Is)] males are equally competitive with La Réunion males resulting in demographic crash when LR[wPip(Is)] males were introduced into La Réunion laboratory cages. These results, together with the geographic isolation of the four south-western Indian Ocean islands and their limited land area, support the feasibility of an IIT program using LR[wPip(Is)] males and stimulate the implementation of field tests for a Cx. p. quinquefasciatus control strategy on these islands. PMID:22206033

  5. Cytoplasmic incompatibility as a means of controlling Culex pipiens quinquefasciatus mosquito in the islands of the south-western Indian Ocean.

    Célestine M Atyame

    2011-12-01

    Full Text Available The use of the bacterium Wolbachia is an attractive alternative method to control vector populations. In mosquitoes, as in members of the Culex pipiens complex, Wolbachia induces a form of embryonic lethality called cytoplasmic incompatibility, a sperm-egg incompatibility occurring when infected males mate either with uninfected females or with females infected with incompatible Wolbachia strain(s. Here we explore the feasibility of the Incompatible Insect Technique (IIT, a species-specific control approach in which field females are sterilized by inundative releases of incompatible males. We show that the Wolbachia wPip(Is strain, naturally infecting Cx. p. pipiens mosquitoes from Turkey, is a good candidate to control Cx. p. quinquefasciatus populations on four islands of the south-western Indian Ocean (La Réunion, Mauritius, Grande Glorieuse and Mayotte. The wPip(Is strain was introduced into the nuclear background of Cx. p. quinquefasciatus mosquitoes from La Réunion, leading to the LR[wPip(Is] line. Total embryonic lethality was observed in crosses between LR[wPip(Is] males and all tested field females from the four islands. Interestingly, most crosses involving LR[wPip(Is] females and field males were also incompatible, which is expected to reduce the impact of any accidental release of LR[wPip(Is] females. Cage experiments demonstrate that LR[wPip(Is] males are equally competitive with La Réunion males resulting in demographic crash when LR[wPip(Is] males were introduced into La Réunion laboratory cages. These results, together with the geographic isolation of the four south-western Indian Ocean islands and their limited land area, support the feasibility of an IIT program using LR[wPip(Is] males and stimulate the implementation of field tests for a Cx. p. quinquefasciatus control strategy on these islands.

  6. Magma dynamics at the base of an evolving mafic magma chamber: Incompatible element evidence from the Partridge River intrusion, Duluth Complex, Minnesota, USA

    Chalokwu, Christopher I.; Ariskin, Alexei A.; Koptev-Dvornikov, Evgeny V.

    1996-12-01

    A characteristic feature of the Partridge River intrusion of the Keweenawan Duluth Complex is the approximately fivefold to ninefold increase in the concentrations of incompatible elements in the lower zone compared with cumulates stratigraphically higher. The concentrations of incompatible elements decrease from the lower zone upward to steady state values, which is ascribed to variations in the proportions of trapped liquid rather than variable degrees of fractional crystallization of a single parental magma. The calculated average composition of trapped liquid using our algorithm is similar to typical Keweenawan low-alumina, high Tisbnd P basalts associated with the Duluth Complex but is different from the leading edge ferrodioritic liquid quenched in the chilled margin of the intrusion. This difference suggests that the chilled margin does not represent the original (parental) magma composition from which the whole intrusion solidified, and that the enrichment of incompatible elements may be related to the local flotation of magmatic suspensions. To test the latter hypothesis numerically, we have used heat-mass transfer models, assuming a sheet-like magma chamber, to calculate the parameters of the model that best reproduce the observed distribution of incompatible elements in a mush zone at the base of the Partridge River intrusion. The results indicate that a mush zone enriched in the incompatible elements is produced if the velocity of movement of the lower solidification front into the magma body was less than the floating velocity of the bulk crystal mush. The dynamic parameters that best reproduce the observed distribution of incompatible elements include a magma emplacement pressure of 2 kbar, critical crystallinities of 50-68% in the mush zone from which the liquid is being expelled, and an emplacement temperature of ˜ 1160°C for the initial magma.

  7. Histology and Ultrastructure of Incompatible Combination Between Puccinia striiformis and Wheat Cultivars with Low Reaction Type Resistance

    KANG Zhen-sheng; WANG Yao; HUANG Li-li; WEI Guo-rong; ZHAO Jie

    2003-01-01

    The incompatible combinations between races of Puccinia striiformis and wheat cuitivars withlow reaction type resistance were examined by means of fluorescent microscopy, differential interference con-trast microscopy and electron microscopy. The incompatible combinations consisted of wheat cultivar Niuzhuteplus CY28, Hybrid46 plus CY29 and Tianxuan882 plus CY29, while the compatible combination was wheatcultivar Huixianhong plus CY28. The observation revealed a striking difference in the fungal development andthe host responses between susceptible and resistant wheat cuitivars following infection by the pathogen. Themain histological manifestation of the pathogen development in the resistant wheat cultivars include inhibitionof hyphal growth, delay of hyphal branching and colony formation, decrease of formation of haustorial mothercells and haustoria, and occurrence of host cell necrosis. The observation by electron microscopy demonstratedthat a series of abnormal changes occurred in intercellular hyphae, haustorial mother cells and haustoria dur-ing pathogen development in the resistant wheat cultivars. The cytoplasm became more electron-dense and vac-uoles in the cytoplasm increased in number and size. The cell walls of hyphae, haustorial mother cells werethickened irregularly. The organeiles were disorderly distributed in the cytoplasm and the haustorial mothercells and haustoria lost their physiological function. In the final stage of the pathogen development, the inter-cellular hyphae, haustorial mother cells and haustoria became necrotic and collapsed. The structural defensereactions such as formation of cell wall apposition, collar and encasement of haustorium were essentially morepronounced in the infected wheat leaves of the resistant cultivars than in the susceptible one. In addition, therelationship between the host resistance expression and the histological and cytological features occurred in theincompatible combination was discussed in this paper.

  8. Self-pollination and parthenocarpic ability in developing ovaries of self-incompatible Clementine mandarins (Citrus clementina).

    Mesejo, Carlos; Yuste, Roberto; Martínez-Fuentes, Amparo; Reig, Carmina; Iglesias, Domingo J; Primo-Millo, Eduardo; Agustí, Manuel

    2013-05-01

    This study aimed to determine if self-pollination is needed to trigger facultative parthenocarpy in self-incompatible Clementine mandarins (Citrus clementina Hort. ex Tan.). 'Marisol' and 'Clemenules' mandarins were selected, and self-pollinated and un-pollinated flowers from both cultivars were used for comparison. These mandarins are always seedless after self-pollination and show high and low ability to develop substantial parthenocarpic fruits, respectively. The time-course for pollen grain germination, tube growth and ovule abortion was analyzed as well as that for carbohydrates, active gibberellins (GA1 and GA4 ), auxin (IAA) and abscisic acid (ABA) content in the ovary. 'Clemenules' showed higher pollen grain germination, but pollen tube development was arrested in the upper style 9 days after pollination in both cultivars. Self-pollination did not stimulate parthenocarpy, whereas both un-pollinated and self-pollinated ovaries set fruit regardless of the cultivar. On the other hand, 'Marisol' un-pollinated flowers showed greater parthenocarpic ovary growth than 'Clemenules' un-pollinated flowers, i.e. higher ovule abortion rate (+21%), higher fruit set (+44%) and higher fruit weight (+50%). Further, the greater parthenocarpic ability of 'Marisol' paralleled higher levels of GA1 in the ovary (+34% at anthesis). 'Marisol' ovary also showed higher hexoses and starch mobilization, but lower ABA levels (-64% at anthesis). Self-pollination did not modify carbohydrates or GA content in the ovary compared to un-pollination. Results indicate that parthenocarpy in the Clementine mandarin is pollination-independent with its ability to set depending on the ovary hormone levels. These findings suggest that parthenocarpy in fertile self-incompatible mandarins is constitutively regulated. PMID:23002897

  9. Pollen–pistil interactions and self-incompatibility in the Asteraceae: new insights from studies of Senecio squalidus (Oxford ragwort)

    Allen, Alexandra M.; Thorogood, Christopher J.; Hegarty, Matthew J.; Lexer, Christian; Hiscock, Simon J.

    2011-01-01

    Background Pollen–pistil interactions are an essential prelude to fertilization in angiosperms and determine compatibility/incompatibility. Pollen–pistil interactions have been studied at a molecular and cellular level in relatively few families. Self-incompatibility (SI) is the best understood pollen–pistil interaction at a molecular level where three different molecular mechanisms have been identified in just five families. Here we review studies of pollen–pistil interactions and SI in the Asteraceae, an important family that has been relatively understudied in these areas of reproductive biology. Scope We begin by describing the historical literature which first identified sporophytic SI (SSI) in species of Asteraceae, the SI system later identified and characterized at a molecular level in the Brassicaceae. Early structural and cytological studies in these two families suggested that pollen–pistil interactions and SSI were similar, if not the same. Recent cellular and molecular studies in Senecio squalidus (Oxford ragwort) have challenged this belief by revealing that despite sharing the same genetic system of SSI, the Brassicaceae and Asteraceae molecular mechanisms are different. Key cellular differences have also been highlighted in pollen–stigma interactions, which may arise as a consequence of the Asteraceae possessing a ‘semi-dry’ stigma, rather than the ‘dry’ stigma typical of the Brassicaceae. The review concludes with a summary of recent transcriptomic analyses aimed at identifying proteins regulating pollen–pistil interactions and SI in S. squalidus, and by implication the Asteraceae. The Senecio pistil transcriptome contains many novel pistil-specific genes, but also pistil-specific genes previously shown to play a role in pollen–pistil interactions in other species. Conclusions Studies in S. squalidus have shown that stigma structure and the molecular mechanism of SSI in the Asteraceae and Brassicaceae are different. The

  10. Cis-by-Trans regulatory divergence causes the asymmetric lethal effects of an ancestral hybrid incompatibility gene.

    Shamoni Maheshwari

    Full Text Available The Dobzhansky and Muller (D-M model explains the evolution of hybrid incompatibility (HI through the interaction between lineage-specific derived alleles at two or more loci. In agreement with the expectation that HI results from functional divergence, many protein-coding genes that contribute to incompatibilities between species show signatures of adaptive evolution, including Lhr, which encodes a heterochromatin protein whose amino acid sequence has diverged extensively between Drosophila melanogaster and D. simulans by natural selection. The lethality of D. melanogaster/D. simulans F1 hybrid sons is rescued by removing D. simulans Lhr, but not D. melanogaster Lhr, suggesting that the lethal effect results from adaptive evolution in the D. simulans lineage. It has been proposed that adaptive protein divergence in Lhr reflects antagonistic coevolution with species-specific heterochromatin sequences and that defects in LHR protein localization cause hybrid lethality. Here we present surprising results that are inconsistent with this coding-sequence-based model. Using Lhr transgenes expressed under native conditions, we find no evidence that LHR localization differs between D. melanogaster and D. simulans, nor do we find evidence that it mislocalizes in their interspecific hybrids. Rather, we demonstrate that Lhr orthologs are differentially expressed in the hybrid background, with the levels of D. simulans Lhr double that of D. melanogaster Lhr. We further show that this asymmetric expression is caused by cis-by-trans regulatory divergence of Lhr. Therefore, the non-equivalent hybrid lethal effects of Lhr orthologs can be explained by asymmetric expression of a molecular function that is shared by both orthologs and thus was presumably inherited from the ancestral allele of Lhr. We present a model whereby hybrid lethality occurs by the interaction between evolutionarily ancestral and derived alleles.

  11. Lack of Association between ABO, PPAP2B, ADAMST7, PIK3CG, and EDNRA and Carotid Intima-Media Thickness, Carotid Plaques, and Cardiovascular Disease in Patients with Rheumatoid Arthritis

    Raquel López-Mejías

    2014-01-01

    Full Text Available Introduction. Rheumatoid arthritis (RA is a polygenic disease associated with accelerated atherosclerosis and increased cardiovascular (CV mortality. Recent studies have identified the ABO rs579459, PPAP2B rs17114036, and ADAMTS7 rs3825807 polymorphisms as genetic variants associated with coronary artery disease and the PIK3CG rs17398575 and EDNRA rs1878406 polymorphisms as the most significant signals related to the presence of carotid plaque in nonrheumatic Caucasian individuals. Accordingly, we evaluated the potential relationship between these 5 polymorphisms and subclinical atherosclerosis (assessed by carotid intima-media thickness (cIMT and presence/absence of carotid plaques and CV disease in RA. Material and Methods. 2140 Spanish RA patients were genotyped for the 5 polymorphisms by TaqMan assays. Subclinical atherosclerosis was evaluated in 620 of these patients by carotid ultrasonography technology. Results. No statistically significant differences were found when each polymorphism was assessed according to cIMT values and presence/absence of carotid plaques in RA, after adjusting the results for potential confounders. Moreover, no significant differences were obtained when RA patients were stratified according to the presence/absence of CV disease after adjusting for potential confounders. Conclusion. Our results do not confirm association between ABO rs579459, PPAP2B rs17114036, ADAMTS7 rs3825807, PIK3CG rs17398575, and EDNRA rs1878406 and subclinical atherosclerosis and CV disease in RA.

  12. Un debate metodológico entre dos aproximaciones al estudio de caso: Caso de conciencia frente a estudio de caso histórico-humanista

    Fernández Cano, Antonio; Lara Moreno, Teresa; de Dios Melgarejo Jaldo, Juan; Bueno Sánchez, Angel

    2002-01-01

    Not available

    Este estudio considera dos aproximaciones a un método de investigación tan complejo como el estudio de caso. Por un lado, el antiguo y seminal enfoque del caso de conciencia, se considera con profundidad. Por otro, también desarrolla conceptual y metodológicamente una de las diversas acepciones actuales al estudio de caso, la histórico-humanista según propuso Caro Baroja. El objetivo central de este trabajo podría ser presentar la aproximación histórico-humani...

  13. Frecuencias de grupos sanguíneos e incompatibilidades ABO y RhD, en La Paz, Baja California Sur, México

    del Peón-Hidalgo Lorenzo

    2002-01-01

    Full Text Available Objetivo. Determinar las frecuencias génicas y de fenotipo, y predecir el riesgo de incompatibilidad y aloinmunización materna en la población de La Paz, Baja California Sur, México. Material y métodos. Estudio descriptivo en el que se evaluaron 1 809 disponentes sanguíneos altruistas que acudieron en 1998 al Hospital General de Zona, del Instituto Mexicano del Seguro Social en La Paz, Baja California Sur, México, tipificados por aglutinación en tubo. Las frecuencias génicas fueron estimadas asumiendo condiciones de equilibrio, y las incompatibilidades y riesgo de aloinmunización de acuerdo con la literatura. Los datos fueron evaluados estadísticamente mediante ji². Resultados. La población reveló las siguientes frecuencias en porcentaje: O, 58.49; A, 31.40; B, 8.40; AB, 1.71; RhD, 95.36; y RhD negativo, 4.64 y las frecuencias génicas fueron i, 0.7648; I A, 0.1821; I B, 0.0519; D, 0.7845; d, 0.2155, respectivamente. Las incompatibilidades en parejas y maternofetal fueron 0.3023 y 0.1685 para ABO, 0.0442 y 0.0364 para RhD y 0.0134 y 0.0061 para incompatibilidad doble, respectivamente. La probabilidad de aloinmunización materna fue estimada en 0.0309. Conclusiones. En La Paz, los grupos O y RhD son los más abundantes, aunque las frecuencias están entre las más bajas en México, contrario a lo ocurrido para A y RhD negativo. La probabilidad de aloinmunización materna y las incompatibilidades son también elevadas. Los grupos ancestrales blanco, negro e indio interactuaron en la zona noroeste del país, determinando al migrar a Baja California Sur un mestizaje probablemente similar al resto de la zona noroeste.

  14. Comprometimento medular por linfoma tipo Burkitt: relato de um caso

    Murillo Côrtes Drummond

    1985-06-01

    Full Text Available Registro de caso de linfoma tipo Burkitt com comprometimento intrarraqueano em criança de três anos de idade. Considerações sobre esse tipo de tumor são feitas em função do caso observado e de dados da literatura.

  15. Qualidade da energia eléctrica : casos reais

    Afonso, João L.; Sousa, Tiago N.; Pregitzer, Ricardo G.; Júlio S Martins

    2005-01-01

    Este artigo relata e analisa alguns exemplos de casos reais de problemas de qualidade de energia eléctrica observados em diferentes instalações eléctricas. São ainda apontadas soluções para cada um dos casos.

  16. Leiomioma perianal. Reporte de un caso

    Yorlenis C Uzcátegui R

    2014-09-01

    Full Text Available Los leiomiomas perianales son tumoraciones derivadas de células mesenquimales distribuidas por todo el cuerpo. Histológicamente se han clasificado en tres subtipos: vascular, no vascular y epiteloide. El subtipo vascular puede ser sólido, cavernoso o venoso. Los leiomiomas son raros en adultos, con incidencia de 0,1 %, generalmente se presentan entre la cuarta a sexta década de la vida con predominio en mujeres en caso de ser benignos. La forma de presentación más frecuente es un nódulo palpable, próximo al ano e indoloro. El diagnóstico se realiza según criterios histológicos e inmunohistoquímicos, además son útiles la ecografía endoanal y RMN. La exéresis es la primera opción terapéutica. Se presenta caso de paciente femenino de 41 años de edad, quien consulta por presentar nódulo palpable perianal derecho, con dolor a la palpación, de ocho meses de evolución, con valores normales de marcadores tumorales, el ultrasonido revela a nivel perianal derecho imagen sólida, hipocrómica (con tejido lineal fibroso, mide 4.82x 6.7x 6.87cm, la RMN muestra lesión ocupante de espacio en la misma ubicación, Doppler Color sin vascularización. Se efectuó exéresis de leiomioma en fosa isquiorectal derecha de 4x6 cm. El estudio anatomopatológico reportó leiomioma perianal subtipo epitelial. En el presente estudio se reporta un caso muy poco frecuente de leiomioma perianal doloroso, exponiendo las características clínicas e histológicas del mismo

  17. Babesiose cerebral em bovinos: 20 casos

    Rodrigues Aline

    2005-01-01

    Full Text Available Num estudo retrospectivo de 1.071 necropsias de bovinos, foram encontrados 20 casos de babesiose cerebral confirmados pelo exame de esfregaços do córtex telencefálico. A maioria desses casos ocorreu no verão, em bovinos adultos de raças européias ou suas cruzas. Além dos sinais clínicos neurológicos, observou-se hemoglobinúria, anorexia, febre, taquicardia, taquipnéia e queda na produção de leite. O hemograma realizado em cinco bovinos afetados revelou acentuada anemia regenerativa; em média, 20% dos eritrócitos estavam parasitados por Babesia bovis. Na necropsia de todos os casos, a substância cinzenta dos córtices telencefálico e cerebelar e dos núcleos da base era consistente e caracteristicamente róseo-cereja. Essa coloração devia-se ao seqüestro de eritrócitos nos capilares encefálicos. Nesses eritrócitos, podiam-se observar, tanto em esfregaços do córtex como em preparações histológicas do encéfalo, microorganismos com morfologia compatível com B. bovis. Outros achados de necropsia incluíam hemoglobinúria, rins vermelho-escuros, hepatoesplenomegalia, fígado vermelho-alaranjado, icterícia, hemorragias epicárdicas e endocárdicas e edema e congestão dos linfonodos mesentéricos.

  18. Pielite enfisematosa unilateral: relato de caso

    Eduardo Gonçalves

    2013-02-01

    Full Text Available Relata-se um caso de paciente de 22 anos de idade, gênero feminino, que foi admitida no pronto-socorro com lombalgia aguda, disúria, vômitos e febre (38,5ºC. Os achados de exames de urina e tomografia computadorizada demonstraram infecção no trato urinário associada a gás no sistema coletor, configurando pielite enfisematosa unilateral por Gram-negativo. O presente relato enfatiza a ocorrência deste agravo como complicação de infecção no trato urinário.

  19. Caso Clínico en Lectoescritura

    Conde González, María

    2015-01-01

    Este trabajo, intenta plasmar el inicio, el desarrollo y los resultados obtenidos de un caso clínico en concreto, el de una persona adulta con discapacidad intelectual leve, en el que llevamos a cabo una intervención logopédica centrada en le lectura y escritura dándole un uso más funcional a su vida diaria. Después de una observación detallada y minuciosa, se efectúa una evaluación, realizada con diferentes pruebas tales como el Revelsal Test, Pro-lec y Pro-esc. Y para finalizar, se desar...

  20. Hidatidosis muscular primaria: reporte de un caso

    Inés Natividad; Juan Carlos Ferrufino; Alcides García; Ciro Maguiña; César Ramírez

    2009-01-01

    Se presenta el caso de una mujer saludable de 28 años con un tumor en la pierna dependiente de tejidos blandos, no tiene antecedentes epidemiológicos de viajes fuera de Lima Metropolitana, sin compromiso pulmonar, hepático o sistémico y con serología negativa para Equinococcus granulossus antes, durante y después de la extirpación de la lesión. En una primera oportunidad se realiza la extracción del tumor y no se sospecha de hidatidosis por lo que no se le dio el manejo adecuado, 16 semanas d...

  1. Las invaginaciones dentarias. Estudio de 127 casos

    Miguel, A.; Macorra García, José Carlos de la

    1993-01-01

    El dens invaginatus o dens in dente es un trastorno del desarrollo producido por la invaginación del epitelio dentario. Se realiza una revisión bibliográfica sobre este defecto dentario y se estudian 127 casos recogidos de la literatura desde 1949 a 1990. Se concluye que la anomalía se diagnostica preferentemente en jóvenes, que es el doble de frecuente en hombres que en mujeres, que no siempre provoca clínica, que aunque puede aparecer en cualquier diente la pieza que se afecta con más...

  2. Estudo molecular de casos de tuberculose recorrente

    Alves, Adriana Judite Resenda

    2008-01-01

    A tuberculose é uma doença infecto-contagiosa, cujo agente etiológico é o Mycobacterium tuberculosis, que tem permanecido, até aos dias de hoje, como um sério problema mundial de saúde pública. Depois de ter sido considerada sob controlo, a tuberculose ressurgiu de uma forma bastante preocupante, na medida em que o número de casos da doença aumentou drasticamente devido à co-infecção com o Vírus da Imunodeficiência Humana (VIH). Outros factores, também importantes, como o aparecimento de esti...

  3. Anorexia nervosa: un estudio de casos

    Lillyana Zusman Tinman

    2013-01-01

    La Anorexia Nervosa es un trastorno de alimentación que se define (etimológicamente) como una "pérdida nerviosa del apetito". Se caracteriza por la actitud consciente, voluntaria y rotunda de los sujetos  de tener un exceso de peso que intentan modificar por vía de la inanición. A partir del estudio de casos, se propone la distinción entre una Anorexia Nervosa Estructural -aquella en la que predomina el conflicto intrapsíquico primario y arcaico, y que manifiesta una conducta aislada y retraí...

  4. Granuloma esquistossomotico medular: registro de um caso

    Lamartine Corrêa de Moraes Junior

    1984-09-01

    Full Text Available Os autores relatam um caso de granuloma esquistossomótico de localização medular cujo diagnóstico inicial, foi de tumor intramedular sem caracterização nosológica. O resultado e diagnóstico, após exame histológico da peça cirúrgica, bem como aspectos diagnósticos através de exames complementares e características endêmicas do processo são discutidos.

  5. Mixofibrossarcoma: relato de caso Mixofibrossarcoma: case report

    Walquíria Lima Tupinambá; Renata Almeida Schettini; Januário de Souza Júnior; Antonio Pedro Mendes Schettini; Carlos Alberto Chirano Rodrigues; Flaviano da Silva Oliveira

    2011-01-01

    O mixofibrossarcoma, previamente conhecido como histiocitoma fibroso maligno, variante mixoide, é um tumor raro, de origem mesenquimal, composto por células fusiformes e estroma mixoide. Acomete mais idosos, envolvendo extremidades inferiores e estendendo-se, em sua maior parte, até a derme e o subcutâneo. Apresenta altas taxas de recorrência e para seu diagnóstico é fundamental a realização de uma biópsia profunda. Relataremos o caso de um mixofibrossarcoma de alto grau, caracterizado por le...

  6. Hidrocefalia multiloculada: relato de dois casos

    Marilisa M. Guerreiro

    1988-09-01

    Full Text Available Hidrocefalia multiloculada é conceituada como quadro clínico-patológico que consiste de ventrículos aumentados e loculados e de cavidades porencefálicas paraventriculares. Apresentamos dois casos de hidrocefalia multiloculada, um provavelmente conseqüente a processo infeccioso do sistema nervoso central e outro em decorrência de malformação congênita. Aspectos tomográficos compatíveis ao diagnóstico são ressaltados. A etiopatogenia, o tratamento e o prognóstico são discutidos em face da literatura compulsada.

  7. Apoyo conductual positivo: estudio de un caso

    Redondo Redondo, Patricia

    2015-01-01

    Este trabajo se ha realizado con el propósito de investigar el programa de Apoyo Conductual Positivo (APC). Específicamente en este trabajo presentamos el estudio de un caso, un alumno con trastorno del espectro autista escolarizado en un centro de educación especial. Para ello se ha realizado una revisión bibliográfica de la discapacidad, se ha conceptualizado la discapacidad intelectual y los trastornos del espectro autista, se ha repasado brevemente los autores más representativos de la mo...

  8. Filariosis humana en la selva peruana: reporte de tres casos

    María Beltrán; Gabriela Cancrini; Guillermo Reátegui; Raúl Melgar; Carlos Ayllón; María del Carmen Garaycochea; Rosalina Reyes; Lammie, Patrick J

    2008-01-01

    Se presenta tres casos de filariosis de pacientes varones procedentes de la selva peruana (Junín, San Martín y Pucallpa). Un caso presentó filarias en el globo ocular y frotís sanguíneo, que según morfología, serología y biología molecular se determinó como un posible caso de filariosis zoonótica por Onchocerca spp. Los otros dos casos fueron causados por Dirofilaria spp. uno presentó un nódulo en el pómulo y sensación de movilidad en la zona y fue diagnosticado por serología y el último caso...

  9. In the Eyes of the Beholder: Understanding and Resolving Incompatible Ideologies and Languages in US Environmental and Cultural Laws in Relationship to Navajo Sacred Lands

    Milholland, Sharon

    2010-01-01

    In this article, the author raises a few examples of incompatible concepts and languages in US federal environmental and cultural laws affecting the management of indigenous sacred lands. She explains these examples by describing the management of a selection of Navajo (Dine) sacred places and elsewhere. Through fundamental concepts rooted in…

  10. Pig BMSCs Transfected with Human TFPI Combat Species Incompatibility and Regulate the Human TF Pathway in Vitro and in a Rodent Model

    Hongchen Ji

    2015-05-01

    Full Text Available Background: The activation of tissue factor (TF is one of the major reasons for coagulation dysregulation after pig-to-primate xenotransplantation. Tissue factor pathway inhibitor (TFPI is the most important inhibitor of TF. Studies have demonstrated species incompatibility between pig TFPI and human TF. Methods: A pig-to-macaque heterotopic auxiliary liver transplantation model was established to determine the origin of activated TF. Chimeric proteins of human and pig TFPI were constructed to assess the role of Kunitz domains in species incompatibility. Immortalised pig bone marrow mesenchymal stem cells transfected with human TFPI were tested for their ability to inhibit clotting in vitro. Results: TF from recipient was activated early after liver xenotransplantation. Pig TFPI Kunitz domain 2 bound human FXa, but Kunitz domain 1 did not effectively inhibit human TF/FVIIa. Immortalised pig bone marrow mesenchymal cells (BMSCs transfected with human TFPI showed a prolonged recalcification time in vitro and in a rodent model. Conclusion: Recipient TF is relevant to dysregulated coagulation after xenotransplantation. Kunitz domain 1 plays the most important role in species incompatibility between pig TFPI and human TF, and clotting can be inhibited by human TFPI-transfected pig BMSCs. Our study shows a possible way to resolve the incompatibility of pig TFPI.

  11. Comparative transcriptome analysis of stylar canal cells identifies novel candidate genes implicated in the self-incompatibility response of Citrus clementina

    Caruso Marco

    2012-02-01

    Full Text Available Abstract Background Reproductive biology in citrus is still poorly understood. Although in recent years several efforts have been made to study pollen-pistil interaction and self-incompatibility, little information is available about the molecular mechanisms regulating these processes. Here we report the identification of candidate genes involved in pollen-pistil interaction and self-incompatibility in clementine (Citrus clementina Hort. ex Tan.. These genes have been identified comparing the transcriptomes of laser-microdissected stylar canal cells (SCC isolated from two genotypes differing for self-incompatibility response ('Comune', a self-incompatible cultivar and 'Monreal', a self- compatible mutation of 'Comune'. Results The transcriptome profiling of SCC indicated that the differential regulation of few specific, mostly uncharacterized transcripts is associated with the breakdown of self-incompatibility in 'Monreal'. Among them, a novel F-box gene showed a drastic up-regulation both in laser microdissected stylar canal cells and in self-pollinated whole styles with stigmas of 'Comune' in concomitance with the arrest of pollen tube growth. Moreover, we identify a non-characterized gene family as closely associated to the self-incompatibility genetic program activated in 'Comune'. Three different aspartic-acid rich (Asp-rich protein genes, located in tandem in the clementine genome, were over-represented in the transcriptome of 'Comune'. These genes are tightly linked to a DELLA gene, previously found to be up-regulated in the self-incompatible genotype during pollen-pistil interaction. Conclusion The highly specific transcriptome survey of the stylar canal cells identified novel genes which have not been previously associated with self-pollen rejection in citrus and in other plant species. Bioinformatic and transcriptional analyses suggested that the mutation leading to self-compatibility in 'Monreal' affected the expression of non

  12. Vasculitis por Propiltiouracilo: reporte de un caso

    Donato A. Salas-Segura

    2002-06-01

    Full Text Available Se reporta el caso clínico de una paciente femenina de 43 años que presentó dos complicaciones secundarias al uso de propiltiouracilo: vasculitis y agranulocitosis.La vasculitis asociada con el tratamiento antitiroideo es una entidad clínica bien documentada, pero rara, y de un mecanismo patogénico no claro aún. Hay pocos casos reportados de asociación entre la ingesta de propiltiouracilo, la aparición de anticuerpos anticitoplasmáticos antineutrófilos y vasculitis ANCA positiva. Este es probablemente uno de ellos.We report a case of 43-year-old woman who developed two complications associated with the use of propylthiouracil: vasculitis and agranulocytosis. Vasculitis associated with antithyroid therapy is a rare well-documented clinical entity with a pathogenic mechanism not clear yet. There a few published reports of an association between treatment with propylthiouracil, and the occurrence of ANCA positivity and ANCA-associated vasculitis. This is probably one more.

  13. Sarcoma sinovial anorretal: relato de caso

    Hernán Augusto Centurión Sobral

    2006-03-01

    Full Text Available Os sarcomas são neoplasias que se originam das células mesenquimais primitivas, sendo raros na região anorretal. O objetivo é relatar um caso de sarcoma sinovial anorretal, neoplasia extremamente rara nesta localização. É descrito o caso de uma paciente de 77 anos que apresentava nodulação anal dolorosa e sangrante às evacuações, associada a puxo, tenesmo e perda ponderal. A lesão foi biopsiada e o estudo imunohistoquímico evidenciou sarcoma sinovial anorretal. A paciente foi submetida a amputação abdomino-perineal do reto, encontra-se assintomática, sem sinais de recidiva e em seguimento ambulatorial.Originated from mesenchymal cells, the sarcoma is rare in the anorectal area. The authors report a case of anorectal sinovial sarcoma, extremely rare in this location with no previous reports on literature. It's described a case of a 77 years old patient presenting an anal tumor, associated to pain, bleeding, tenesmus and weight loss. A synovial sarcoma was diagnosed after biopsy and imunohistochemical study. The pacient was submitted to a Miles procedure being assymptomatic and without any signs of disease in the last seven months.

  14. Cistos de Tarlov: relato de quatro casos

    Sá Márcia Cristina da Paixão Rodrigues Miranda de

    2004-01-01

    Full Text Available São relatados quatro casos de cistos perineurais sacrais, conhecidos por cistos de Tarlov, e é feita revisão da literatura. A partir de amostra de 88 pacientes com queixas de radiculopatia em membros inferiores, e/ ou dor lombar ou sacral que foram submetidos à ressonância magnética de coluna lombossacra quatro (4.5% tiveram o diagnóstico de cisto de Tarlov. O diagnóstico foi feito através de ressonância magnética de coluna vertebral lombossacra. Os quatro casos foram submetidos a laminectomia sacral. Todos evoluíram assintomáticos sem défices motores ou sensitivos. Os cistos de Tarlov podem apresentar manifestações clínicas semelhantes às provocadas por hérnia discal lombossacra embora, nem sempre sejam citados nos diagnósticos diferenciais. O tratamento clínico é basicamente fisioterápico. O tratamento cirúrgico visa sanar o efeito compressivo em estruturas ósseas e/ ou nervosas através de punção do cisto ou retirada dele através de laminectomia sacral.

  15. Interspecific crossing and genetic mapping reveal intrinsic genomic incompatibility between two Senecio species that form a hybrid zone on Mount Etna, Sicily.

    Brennan, A C; Hiscock, S J; Abbott, R J

    2014-09-01

    Studies of hybridizing species can reveal much about the genetic basis and maintenance of species divergence in the face of gene flow. Here we report a genetic segregation and linkage analysis conducted on F2 progeny of a reciprocal cross between Senecio aethnensis and S. chrysanthemifolius that form a hybrid zone on Mount Etna, Sicily, aimed at determining the genetic basis of intrinsic hybrid barriers between them. Significant transmission ratio distortion (TRD) was detected at 34 (∼27%) of 127 marker loci located in nine distinct clusters across seven of the ten linkage groups detected, indicating genomic incompatibility between the species. TRD at these loci could not be attributed entirely to post-zygotic selective loss of F2 individuals that failed to germinate or flower (16.7%). At four loci tests indicated that pre-zygotic events, such as meiotic drive in F1 parents or gametophytic selection, contributed to TRD. Additional tests revealed that cytonuclear incompatibility contributed to TRD at five loci, Bateson-Dobzhansky-Muller (BDM) incompatibilities involving epistatic interactions between loci contributed to TRD at four loci, and underdominance (heterozygote disadvantage) was a possible cause of TRD at one locus. Major chromosomal rearrangements were probably not a cause of interspecific incompatibility at the scale that could be examined with current map marker density. Intrinsic genomic incompatibility between S. aethnensis and S. chrysanthemifolius revealed by TRD across multiple genomic regions in early-generation hybrids is likely to impact the genetic structure of the natural hybrid zone on Mount Etna by limiting introgression and promoting divergence across the genome. PMID:24595365

  16. DYNAMICS OF INSULIN-LIKE GROWTH FACTOR-1 (IGF-1 IN CHILDREN AFTER AB0-INCOMPATIBLE LIVER TRANSPLANTATION

    O.P. Shevchenko

    2014-01-01

    Full Text Available It is shown that liver transplantation (LT from donor with incompatible blood type (AB0i may be effective and safe, but the impact of such operation upon the various systems of the body has not been investigated yet. Insulinlike growth factor-1 (IGF-1 is synthesized in the liver and mediates the action of growth hormone. The level of IGF-1 is a marker of the processes of cell proliferation and tissue regeneration. Aim. To evaluate levels of IGF-1 in children-recipients with liver transplant from AB0i (incompatible and AB0c (compatible donors.Materials and methods. 140 children aged 3 to 36 (19,5 ± 16,5 months with congenital diseases of the hepatobiliar system, 58 of them boys, were surveyed. All patients underwent transplantation of left lateral liver sector from living related donors: 111 children were transplanted with fragment of the liver from AB0c donors, 29 – from AB0i donors; in 10 children with AB0i liver before and/or after LT operation anti-group antibodies (anti-A/B were revealed. The concentration of IGF-1 was determined by ELISA using specifi c kits (Immunodiagnostic System, USA in samples of blood plasma, which were received up to a month and a year after a liver transplant.Results. Average level of IGF-1 21,0 ± 29,5 μg/l in patients before LT was signifi cantly lower than in healthy children (52,2 ± 26,3 μg/l, p < 0,001 and did not vary in children, having received later a piece of liver from a compatible (AB0c donor and from donor AB0i (23,5 ± 30,9 and 21,2 ± 23,2 μg/l respectively, p = 0,70. In patients with anti-A/B prior to surgery average level of IGF-1 was not different from that of the patients without antibodies (32,6 ± 27,6 and 22,3 ± 29,6 μg/l respectively, p = 0,4. One month after LT level of IGF-1 has increased both in the general group, and in patients with AB0c and AV0i liver (92,1 ± 77,8 and 131,2 ± 106,7 μg/l respectively, p = 0,09. The level of IGF-1 was not varied in the group with antibodies

  17. A study on Rh incompatibility and frequency of weak D among blood donors and patients at a tertiary care referral teaching hospital in Tirupati, Andhra Pradesh

    Deepthi Krishna G

    2015-10-01

    Full Text Available Background: Rh D antigen is the next most important after ABO antigens in the field of transfusion medicine. Weak D refers to reduced expression of D antigen on the red blood cell that requires an extended testing with indirect antiglobulin test (IAT to get detected. Clinical importance of weak D arises when labelling the donor and patient, as the donor is labelled as D positive, patient as D negative. Methods: In our center all blood donor and patient samples are tested for ABO and Rh D by conventional tube technique using two anti-D reagents; anti-D immunoglobulin M (IgM monoclonal and a blend of anti-D IgM and immunoglobulin G (IgG. The blood samples which were negative for agglutination by immediate spin method were further tested for weak-D using IgG anti-D in the IAT phase with low ionic strength solution (LISS/Coombs’gel card. Results: A total of 46,654 blood samples were tested (22,326 donors and 24,328 patients during the period January 2012 to August 2014. Among these 43,771 (93.82% were Rh D positive and remaining 2,883 (6.18% were Rh-D negative. A total of 30 individuals (16 donors and 14 patients were weak D positive constituting 1.04% of Rh-D negatives and 0.06% of total individuals screened. Conclusions: This study shows the prevalence of weak D antigen in our population who are representative of Rayalaseema region of Andhra Pradesh. It also stresses the need to identify individuals with variant D (rather than weak or partial D and to inform them about their status as donor and recipient of blood/organ.

  18. Politica de gratuidad: un estudio de caso

    Everly Castellar Paternina

    2015-01-01

    Full Text Available Este artículo presenta los resultados de una investigación con diseño de estudio de caso de corte cualitativo, cuyo propósito fue analizar, en el marco de la política pública en Colombia, los alcances de la implementación de la gratuidad en una Institución educativa (IE oficial en Barranquilla en el periodo 2008-2012. En la metodología se combinan técnicas cualitativas, como la entrevista semiestructurada, y técnicas cuantitativas como un cuestionario “Ad hoc”. Se hizo un análisis documental y de contenido de los documentos expedidos por el Ministerio de Educación Nacional y la IE. Se aplicó la técnica del análisis de regresión simple para revisar el comportamiento de las variables independientes en este caso gratuidad y las variables dependientes como son cobertura, matrícula y deserción que permitiera realizar un contraste de los alcances y efectos de la gratuidad en la IE objeto de estudio. Por último, se realizó una triangulación con el fin de unir las diferentes técnicas para que nos arrojaran los resultados de esta investigación que en este caso indicaron que: i. La gratuidad se aplica conforme a los lineamientos del decreto 4807 de 2011; ii. Los lineamientos para la implementación y el seguimiento a la gratuidad no se comunican a los padres de familia; iii. Los docentes no tienen claridad sobre la destinación específica de los rubros de calidad; iv. La deserción no tiene una relación directa con la política de gratuidad y la gratuidad no tiene relación directa con la reducción de la tasa de deserción; v. La IE debe mejorar el sistema de comunicación con los padres; vi. La gratuidad está más relacionada con el acceso que con la calidad de los aprendizajes.

  19. Ataxia espinocerebelar tipo 6: relato de caso

    Bianca Simone Zeigelboim

    2014-10-01

    Full Text Available O objetivo deste estudo foi verificar as alterações vestibulococleares observadas em um caso de ataxia espinocerebelar tipo 6. O caso foi encaminhado do Hospital de Clínicas para o Laboratório de Otoneurologia de uma Instituição de Ensino e foi submetido aos seguintes procedimentos: anamnese, inspeção otológica, avaliações audiológica e vestibular. O caso retrata uma paciente com diagnóstico genético de ataxia espinocerebelar tipo 6, do sexo feminino, com 57 anos de idade, que referiu desequilíbrio à marcha com tendência a queda para a esquerda, disartria e disfonia. Na avaliação audiológica apresentou configuração audiométrica descendente a partir da frequência de 4kHz e curva timpanométrica do tipo "A" com presença dos reflexos estapedianos bilateralmente. No exame vestibular observou-se na pesquisa da vertigem posicional presença de nistagmo vertical inferior e oblíquo, espontâneo e semiespontâneo múltiplo com características centrais (ausência de latência, paroxismo, fatigabilidade e vertigem, nistagmooptocinético abolido e hiporreflexia à prova calórica. Constataram-se alterações labirínticas que indicaram afecção do sistema vestibular central evidenciando-se a importância dessa avaliação. A existência da possível relação entre os achados com os sintomas vestibulares apresentados pela paciente apontou a relevância do exame labiríntico neste tipo de ataxia uma vez que a presença do nistagmo vertical inferior demonstrou ser frequente neste tipo de patologia.

  20. Manual de casos administrativos para el sector agrario

    Sánchez Zapata, Blanca Eugenia

    2006-01-01

    El trabajo es una aplicación del "método de casos", dentro del cual se desarrollan seis de ellos, complementados con la teoría e información pertinente. El método de casos, es una herramienta de docencia – aprendizaje ampliamente conocida y utilizada en varias universidades para la formación de administradores y otras profesiones. Para los casos del presente trabajo, se seleccionaron los temas teóricos que muestran la mayor diferencia metodológica en su aplicación a las empresas agrarias con ...

  1. Carcinoma de pele no segmento craniofacial: Relato de 3 casos

    Iana Lara Pereira Maia; Joaquim José Silva; Hafiza Gonçalves Alexandrino Regino; Khadija Neide Alexandrino Regino; Liana Barbosa e Sila; Sônia Araújo Rogério Nunes

    2014-01-01

    O câncer de pele é a neoplasia mais frequente no Brasil. O tipo histológico mais comum é o carcinoma basocelular (CBC), 70% dos casos, seguido pelo carcinoma espinocelular (CEC) e pelo melanoma. Relatamos três carcinomas de pele no segmento crânio facial, evidenciando as abordagens cirúrgicas realizadas. No caso 1, apresenta-se um CBC em hemiface submetido à exérese com retalho V-Y. No caso 2, destaca-se um CEC em couro cabeludo submetido à exérese com retalhos de rotação e da vizinhança. No ...

  2. UMA TEORÍA CAUSAL PARA LOS CASOS FREGE

    ABEL WAJNERMAN PAZ

    2015-01-01

    Fodor ha argumentado a favor de un par de tesis que pueden caracterizarse como constituyendo un dilema: Por un lado, si adoptamos una teoría funcional para los conceptos explicamos semánticamente los casos Frege pero caemos en el holismo semántico. Por otro lado, si adoptamos una teoría causal/informacional evitamos el holismo pero no explicamos los casos Frege semánticamente. Fodor (por ej, 1994, 1998 y 2008) intenta evitar la segunda parte del dilema argumentando que los casos de Frege pued...

  3. Evolutionary divergence of the plant elicitor peptides (Peps) and their receptors: interfamily incompatibility of perception but compatibility of downstream signalling

    Lori, M.

    2015-05-22

    Plant elicitor peptides (Peps) are potent inducers of pattern-triggered immunity and amplify the immune response against diverse pathogens. Peps have been discovered and studied extensively in Arabidopsis and only recently orthologs in maize were also identified and characterized in more detail. Here, the presence of PROPEPs, the Pep precursors, and PEPRs, the Pep receptors, was investigated within the plant kingdom. PROPEPs and PEPRs were identified in most sequenced species of the angiosperms. The conservation and compatibility of the Pep-PEPR-system was analysed by using plants of two distantly related dicot families, Brassicaceae and Solanaceae, and a representative family of monocot plants, the Poaceae. All three plant families contain important crop plants, including maize, rice, tomato, potato, and canola. Peps were not recognized by species outside of their plant family of origin, apparently because of a divergence of the Pep sequences. Three family-specific Pep motifs were defined and the integration of such a motif into the Pep sequence of an unrelated Pep enabled its perception. Transient transformation of Nicotiana benthamiana with the coding sequences of the AtPEPR1 and ZmPEPR1a led to the recognition of Pep peptides of Brassicaceae or Poaceae origin, respectively, and to the proper activation of downstream signalling. It was concluded that signalling machinery downstream of the PEPRs is highly conserved whereas the leucine-rich repeat domains of the PEPRs co-evolved with the Peps, leading to distinct motifs and, with it, interfamily incompatibility.

  4. Incompatibility between the principle of the constancy of the speed of light and the Lorentz contraction in the GPS Experiment

    Sato, M

    2007-01-01

    Incompatibility between the principle of the constancy of the speed of light and the Lorentz contraction in the global positioning system (GPS) is discussed. The GPS works precisely in the earth-centered locally inertial (ECI) coordinate system on the condition that the speed of light c is assumed to be constant regardless of the inertial motion of the GPS satellites and the earth. The inertial system of the earth travels not only in the solar system at the velocity 30 km/s but also in the cosmic microwave background (CMB) at the velocity 700 km/s. The deviation on the car navigation system by the Lorentz contraction of 700 km/s is maximally estimated as 54 m. However, such a large deviation is not observed: that is, the Lorentz contraction is not observed in the gravitational field of the earth. If there is a Lorentz contraction, the GPS cannot work so precisely. The GPS satellites are in the gravitational field of the earth, therefore the system should be interpreted by the theory of general relativity as w...

  5. H-Y Antigen Incompatibility Not Associated with Adverse Immunologic Graft Outcomes: Deceased Donor Pair Analysis of the OPTN Database

    Douglas Scott Keith

    2011-01-01

    Full Text Available Background. H-Y antigen incompatibility adversely impacts bone marrow transplants however, the relevance of these antigens in kidney transplantation is uncertain. Three previous retrospective studies of kidney transplant databases have produced conflicting results. Methods. This study analyzed the Organ Procurement and Transplantation Network database between 1997 and 2009 using male deceased donor kidney transplant pairs in which the recipient genders were discordant. Death censored graft survival at six months, five, and ten years, treated acute rejection at six months and one year, and rates of graft failure by cause were the primary endpoints analyzed. Results. Death censored graft survival at six months was significantly worse for female recipients. Analysis of the causes of graft failure at six months revealed that the difference in death censored graft survival was due primarily to nonimmunologic graft failures. The adjusted and unadjusted death censored graft survivals at five and ten years were similar between the two genders as were the rates of immunologic graft failure. No difference in the rates of treated acute rejection at six months and one year was seen between the two genders. Conclusions. Male donor to female recipient discordance had no discernable effect on immunologically mediated kidney graft outcomes in the era of modern immunosuppression.

  6. Molecular and quantitative signatures of biparental inbreeding depression in the self-incompatible tree species Prunus avium.

    Jolivet, C; Rogge, M; Degen, B

    2013-05-01

    Genetic diversity strongly influences populations' adaptability to changing environments and therefore survival. Sustainable forest management practices have multiple roles including conservation of genetic resources and timber production. In this study, we aimed at better understanding the variation in genetic diversity among adult and offspring individuals, and the effects of mating system on offspring survival and growth in wild cherry, Prunus avium. We analysed adult trees and open pollinated seed-families from three stands in Germany at eight microsatellite loci and one incompatibility system locus and conducted paternity analyses. Seed viability testing and seed sowing in a nursery allowed further testing for the effects of pollen donor diversity and genetic similarity between mates on the offspring performance at the seed and seedling stages. Our results were contrasting across stands. Loss of genetic diversity from adult to seedling stages and positive effect of mate diversity on offspring performance occurred in one stand only, whereas biparental inbreeding depression and significant decrease in fixation index from adults to seedlings was detected in two stands. We discussed the effects of stand genetic diversity on the magnitude of biparental inbreeding depression at several life-stages and its consequences on the management of genetic resources in P. avium. PMID:23211795

  7. Pyrosequencing-Based Transcriptome Analysis of the Asian Rice Gall Midge Reveals Differential Response during Compatible and Incompatible Interaction

    Jagadish S. Bentur

    2012-10-01

    Full Text Available The Asian rice gall midge (Orseolia oryzae is a major pest responsible for immense loss in rice productivity. Currently, very little knowledge exists with regard to this insect at the molecular level. The present study was initiated with the aim of developing molecular resources as well as identifying alterations at the transcriptome level in the gall midge maggots that are in a compatible (SH or in an incompatible interaction (RH with their rice host. Roche 454 pyrosequencing strategy was used to develop both transcriptomics and genomics resources that led to the identification of 79,028 and 85,395 EST sequences from gall midge biotype 4 (GMB4 maggots feeding on a susceptible and resistant rice variety, TN1 (SH and Suraksha (RH, respectively. Comparative transcriptome analysis of the maggots in SH and RH revealed over-representation of transcripts from proteolysis and protein phosphorylation in maggots from RH. In contrast, over-representation of transcripts for translation, regulation of transcription and transcripts involved in electron transport chain were observed in maggots from SH. This investigation, besides unveiling various mechanisms underlying insect-plant interactions, will also lead to a better understanding of strategies adopted by insects in general, and the Asian rice gall midge in particular, to overcome host defense.

  8. Abscesso cerebelar por Nocardia: relato de caso

    Paulo H.P. Aguiar

    1995-06-01

    Full Text Available A nocardiose do sistema nervoso central tem alcançado importante destaque no diagnóstico diferencial de patologias tumorais nos últimos anos pelo aumento da incidência de pacientes imunodeficiêntes(pacientes submetidos a quimioterapia, transplante de órgãos ou com a síndrome de imunodeficiência adquirida - SIDA. Os autores descrevem o caso de um paciente com SIDA que desenvolveu nocardiose cerebelar na sua forma tumoral. O paciente foi submetido a craniectomia de fossa posterior para diagnóstico e tratamento. Os achados de neuroimagem e patológicos bem como a conduta cirúrgica são discutidos com base em revisão da literatura.

  9. Hidatidosis muscular primaria: reporte de un caso

    Inés Natividad

    2009-01-01

    Full Text Available Se presenta el caso de una mujer saludable de 28 años con un tumor en la pierna dependiente de tejidos blandos, no tiene antecedentes epidemiológicos de viajes fuera de Lima Metropolitana, sin compromiso pulmonar, hepático o sistémico y con serología negativa para Equinococcus granulossus antes, durante y después de la extirpación de la lesión. En una primera oportunidad se realiza la extracción del tumor y no se sospecha de hidatidosis por lo que no se le dio el manejo adecuado, 16 semanas después regresa por recidiva, donde con un diagnóstico específico se realiza la intervención quirúrgica y el tratamiento con albendazol, seis meses de seguimiento después, la paciente no presentó recidiva.

  10. Miastenia grave distal: relato de caso

    Scola Rosana Herminia; Iwamoto Fábio Massaiti; Mainardi Maraya Annaai Michels; Della-Coletta Marcus Vinicius; Carvalho Gisah; Zavala Jorge Augusto; Werneck Lineu Cesar

    2003-01-01

    Relatamos o caso de uma mulher de 30 anos com quadro de fraqueza muscular nos membros inferiores com predomínio distal com início há 7 anos. Na evolução apresentou fraqueza muscular nos membros superiores. O exame físico mostrava nervos cranianos sem alterações, hipotrofia bilateral de quadriceps e interósseos dos pés, redução da força muscular mais intensa em tibiais anteriores e interósseos dorsais dos pés e reflexos tendinosos globalmente hipoativos. Foi realizado teste de estimulação repe...

  11. Caso para diagnóstico

    Souza Fernanda H. M. de; Werner Betina; Cavalin Leila Cristina; Sato Maurício Shigueru; Serafini Sérgio Z.

    2003-01-01

    Pseudoxantoma elástico perfurante periumbilical é distúrbio adquirido do tecido elástico, que acomete mulheres multíparas. Apresenta-se um caso dessa condição em mulher de 74 anos (G10, P10) mostrando placa amarelada de aspecto reticular com áreas constituídas por pápulas eritêmato-ceratósicas. A histologia demonstra derme reticular com fibras conjuntivas basofílicas e irregulares, além de área de hiperplasia epidérmica, com eliminação de fibras elásticas através de canal repleto de ceratina....

  12. Caso para diagnóstico

    Souza Fernanda H. M. de

    2006-01-01

    Full Text Available Pseudoxantoma elástico perfurante periumbilical é distúrbio adquirido do tecido elástico, que acomete mulheres multíparas. Apresenta-se um caso dessa condição em mulher de 74 anos (G10, P10 mostrando placa amarelada de aspecto reticular com áreas constituídas por pápulas eritêmato-ceratósicas. A histologia demonstra derme reticular com fibras conjuntivas basofílicas e irregulares, além de área de hiperplasia epidérmica, com eliminação de fibras elásticas através de canal repleto de ceratina.

  13. Tremor da escrita: relato de caso

    Denise Hack Nicaretta

    1994-03-01

    Full Text Available O tremor da escrita é distúrbio precipitado por atividade motora específica, geralmente a escrita. Analisamos este caso sob o ponto de vista clínico e terapêutico. O paciente apresentava tremor ao escrever tomando sua letra ilegível; sem qualquer outra alteração neurológica. Não havia antecedentes familiares, metabólicos, endócrinos, iatrogênicos, tóxicos ou traumáticos. No manuseio terapêutico não ocorreu resposta satisfatória ao propranolol, sendo discreta à primidona. A introdução de anticolinérgicos (tri-hexifenidil evidenciou certa melhora na sintomatologia, com redução do tremor no momento da escrita.

  14. Nuevas narrativas informativas: el caso de Storify

    Bella Palomo

    2014-05-01

    Full Text Available El artículo analiza la integración del contenido “pro-am” en los cibermedios españoles, entendido como un nuevo género periodístico potencialmente multimedia que combina material elaborado por periodistas y ciudadanos, y se centra en el caso de la herramienta Storify. Además de aportar el primer censo de medios con cuentas oficiales en la aplicación, la investigación recoge las reflexiones de algunos de sus autores, y describe un panorama de amplios contrastes, en el que destaca el uso irregular y superficial de este tipo de narrativa transmedia.

  15. Analysis of correlation between schizophrenia disease and the distribution of ABO blood type%分析精神分裂症与ABO血型分布的相关性

    李含模

    2015-01-01

    目的 研究精神分裂症中ABO血型与临床分型、家族史等相关因素的关系.方法 对240例符合CCMD-3精神分裂症诊断标准的精神分裂症患者性别、ABO血型、临床分型、家族史、起病年龄进行统计分析.结果 精神分裂症患者ABO血型分布比例与中国人血型分布比较差异无统计学意义(P>0.05),O型频率高于A型;性别、家族史、临床分型及起病年龄在ABO血型分布上比较差异无统计学意义(P>0.05);精神分裂症家族史、临床分型及起病年龄在性别上比较差异无统计学意义(P>0.05);精神分裂症临床分型、起病年龄在家族史上比较差异无统计学意义(P>0.05).结论 精神分裂症血型分布频率O型高于A型.精神分裂症患者在性别、临床分型、起病年龄方面无显著的血型及家族史差异.%Objective To study the relationships between ABO blood type and related factors such as Clinical classiifcation, family history in schizophrenia.Methods 240 patients with a CCMD-3 diagnosis of schizophrenia were collected and we Performed statistical analysis on their sex ABO blood type Clinical classification family history age on set.Results There was no signiifcant difference between the schizophrenic and chinese in ABO blood type distribution proportion (P>0.05), blood type O more often than blood type A. sex,Clinical classiifcation,family history and age on set have no differences in ABO blood type distribution proportion (P>0.05). family history of schizophrenia,Clinical classiifcation and age on set have no differences in sex (P>0.05). Clinical classiifcation of schizophrenia age on set have no differences in family history (P>0.05).Conclusion There are more blood type O than blood type A in schizophrenia. sex,Clinical classiifcation,family history and age on set have no differences in blood type among schizophrenic.

  16. 178 cases of ABO hemolytic disease newborn serological testing and its significance%ABO溶血病新生儿178例血清学检测及其意义

    李明强; 杨晓明

    2015-01-01

    目的:探讨ABO溶血病血清检测的临床应用价值。方法选择我院2013年1月~2015年4月收治的178例ABO溶血病患儿的临床资料,采用玻片法对ABO血型正定型检测,采用微柱凝腔免疫检测进行溶血三项检测。包括直接抗入球蛋白实验、红细胞抗体释放试验、血清游离抗体实验及母体血清IgG抗A或抗B体滴度。结果本组母婴血型为A-O的为68例,占比38.2%;B-O型110例,占比61.8%。对患儿血清三项检测发现,直接抗入球蛋白试验中,阳性患儿占比20.2%,血清游离抗体试验,阳性患儿占比40.4%,红细胞抗体释放试验,阳性患儿占比33.1%。全部患儿母亲血清IgG抗A或抗B体滴度均大于1︰64。全部患儿在给予对症处理后,全部痊愈出院。结论采用溶血三项检测ABO溶血症对于提高早期诊断率具有重要的意义,有利于给予患儿早期治疗干预,改善预后。%Objective To investigate the ABO hemolytic disease sera of clinical value.MethodsClinical data in our hospital from January 2013 to April 2015 were treated 178 cases of ABO hemolytic disease in children, the use of slide method to detect positive stereotypes of ABO blood type, the use of micro-column coagulation chamber immunoassay hemolysis three testing. Including the direct anti-globulin test, release test red cell antibodies, antibody test and maternal serum free serum IgG anti-A or anti-B titers.Results The maternal blood as AO for 68 cases, accounting for 38.2%; BO type 110 cases, accounting for 61.8%. Serum levels of three detected, directed against the globulin test positive children accounted for 20.2%, serum free antibody test positive children accounted for 40.4%, red blood cell antibody release test positive patients accounted for 33.1% . The mothers of children with serum IgG anti-A or anti-B titers greater than 1︰64. All patients in the symptomatic treatment, all cured.ConclusionABO hemolytic disease hemolytic

  17. Hipomelanose de Ito - relato de um caso

    Adriana S. de Almeida

    2001-02-01

    Full Text Available Objetivo: Os autores têm como objetivo relatar um caso de hipomelanose de Ito (HI, uma síndrome neurocutânea rara, com alterações neurológicas e cromossômicas associadas ao comprometimento cutâneo e pneumonias de repetição. Relato do caso: Este relato é referente a um paciente masculino, 1 ano e 11 meses, internado no Hospital Universitário São Vicente de Paulo por broncopneumonia bilateral. Ao exame foram observadas máculas hipocrômicas na pele compatíveis com HI, além de atraso do desenvolvimento neuropsicomotor. O paciente foi submetido à biópsia incisional de pele das lesões do abdômen, eletroencefalograma, ressonância magnética e estudo citogenético. Resultados: Os exames histológico e imunoistoquímico evidenciaram ausência de melanina e diminuição de melanócitos em áreas focais da epiderme, respectivamente. O eletroencefalograma apresentou disfunção córtico-subcortical difusa. A ressonância magnética do encéfalo foi compatível com cisto aracnoídeo em região temporal. O cariótipo evidenciou mosaicismo cromossômico com uma linhagem normal (46,XY e uma linhagem celular que apresentava deleção intersticial nas bandas 22.2 - 24.2 do braço longo do cromossomo 10 (25%. Conclusões: Os autores, com o presente estudo, destacam a importância das lesões de pele na definição etiológica das desordens neuropediátricas.

  18. Larva migrans cutanea: reporte de cuatro casos

    Melvis Vivian Pérez Ruch

    2014-01-01

    Full Text Available Introducción: la larva migrans cutánea es una parasitosis que constituye una zoonosis frecuente en zonas tropicales, producida por la penetración y desplazamiento a través de la piel de larvas de nemátodos, como Ancylostoma brazilensis y Ancylostoma caninum. El cuadro clínico es característico y se diagnostica mediante la observación macroscópica de las lesiones serpiginosas debajo de la piel. Los antecedentes están dados por contacto con tierra o arena contaminadas como ocurre en niños que juegan en esos lugares. Objetivo: reportar los hallazgos de esta parasitosis en infantes pertenecientes a dos áreas de salud del municipio Camagüey, durante el período 2010 - 2013. Caso Clínico: en el laboratorio de Microbiología de la policlínica de especialidades pediátricas, se diagnosticaron cuatro niños con larva migrans cutánea de 1, 2, 7 y 11 años de edad, el primero con localización perineal, el segundo, en la espalda, el tercero en glúteos, miembros inferiores y superiores y el cuarto en región glútea y vulvar. En los cuatro casos las lesiones fueron características y se observó el trayecto ondulado del parásito a través de la piel. Los cuatros pacientes evolucionaron satisfactoriamente con tratamiento antiparásito. Conclusiones: dada la molestia, e irritabilidad que ocasiona esta parasitosis, así como la posible evolución tórpida de la misma es importante que en las áreas de 218 salud se tenga en cuenta para su oportuno diagnóstico y tratamiento satisfactorio en bien de los pacientes afectados.

  19. Argyria - Case report Argiria - Relato de caso

    André Lencastre

    2013-06-01

    Full Text Available A 70-year-old male rural worker was referred to our clinic with widespread grey pigmentation of the skin and nails. The condition had been asymptomatic for its entire duration (5 years. He reported past intranasal application of 10% Silver Vitellinate. A skin biopsy was performed and histology corroborated the clinical diagnosis of Argyria. This case represents a currently rare dermatological curiosity. Although silver colloids and salts have been withdrawn and/or banned by some drug surveillance agencies, they continue to be freely sold and unregulated as food supplements and as ingredients in alternative medicines, thereby risking the emergence of new cases of silver poisoning.Um homem de 70 anos, trabalhador rural, foi referenciado à nossa consulta por dermatose assintomática, com 5 anos de evolução, caracterizada pela pigmentação acinzentada generalizada da pele, mais evidente em áreas fotoexpostas, e das lâminas ungueais. Relatava no passado o uso prolongado de Vitelinato de Prata a 10%, por via nasal. Foi efetuado exame histológico de biopsia cutânea que corroborou o diagnóstico clínico de Argiria. O caso representa uma curiosidade dermatológica, atualmente rara. Apesar de abandonados e/ou proibidos por algumas instituições de farmacovigilância, a prata coloidal e sais de prata continuam a ser comercializados como suplementos alimentares, como parte de medicinas alternativas e sem regulação, podendo fazer ressurgir os casos associados à toxicidade pela prata.

  20. Carcinoma de pele no segmento craniofacial: Relato de 3 casos

    Iana Lara Pereira Maia

    2014-09-01

    Full Text Available O câncer de pele é a neoplasia mais frequente no Brasil. O tipo histológico mais comum é o carcinoma basocelular (CBC, 70% dos casos, seguido pelo carcinoma espinocelular (CEC e pelo melanoma. Relatamos três carcinomas de pele no segmento crânio facial, evidenciando as abordagens cirúrgicas realizadas. No caso 1, apresenta-se um CBC em hemiface submetido à exérese com retalho V-Y. No caso 2, destaca-se um CEC em couro cabeludo submetido à exérese com retalhos de rotação e da vizinhança. No caso 3, relata-se um CBC na região pré-auricular e no dorso nasal submetido à exérese com retalho de Limberg e de Rieger.