The GntR-like protein NorG has been shown to affect Staphylococcus aureus genes involved in the resistance to quinolones and ?-lactams, such as those encoding the NorB and AbcA transporters. To identify the target genes regulated by NorG, we carried out transcriptional profiling assays using S. aureus RN6390 and its isogenic norG::cat mutant. Our data showed that NorG positively affected the transcription of global regulators mgrA, arlS, and sarZ. The three putative drug efflux pump genes most positively affected by NorG were the NorB efflux pump (5.1-fold), the MmpL-like protein SACOL2566 (5.2-fold), and the BcrA-like drug transporter SACOL2525 (5.7-fold). The S. aureus predicted MmpL protein showed 53% homology with the MmpL lipid transporter of Mycobacterium tuberculosis, and the putative SACOL2525 protein showed 87% homology with the bacitracin drug ...
ATP-binding cassette transporters (ABCtransporters) utilize the energy of ATP hydrolysis to translocate an unusually diverse set of substrates across cellular membranes. ABCA4, also known as...Full Text Available
The retina-specific ATP-binding cassette (ABC) transporter, ABCA4, is essential for transport of all-trans-retinal from the rod outer segment discs in the retina and is associated with...Full Text Available
The yeast phosphatidylinositol transfer protein (Sec14p) is required for biogenesis of Golgi-derived transport vesicles and cell viability, and this essential Sec14p requirement is abrogated by inactivation...Full Text Available
Signatures of natural selection occur throughout the human genome and can be detected at the sequence level. We have re-sequenced ABCE1, a host candidate gene essential for...Full Text Available
... Thursday: South Brisbane Sailing Club, Orleigh Park Hill End Terrace, West End. Friday: Booroodabin Bowls Club, 126 Breakfast Creek Road, Newstead. Related Photos Ros Capeness and Jeff Shearin: six months on (Emma Sykes - ABC Local) Map Fig Tree Pocket 4069 Subscribe/RSS Subscribe to ABC Brisbane videos Subscribe to all ABC Local videos Topics: floods, people Locations: brisbane-4000,...
... Thursday: South Brisbane Sailing Club, Orleigh Park Hill End Terrace, West End. Friday: Booroodabin Bowls Club, 126 Breakfast Creek Road, Newstead. Related Photos Six months on: Kerrin Quinn's Story (Emma Sykes - ABC Local) Map Fernvale 4306 Subscribe/RSS Subscribe to ABC Brisbane videos Subscribe to all ABC Local videos Topics: disasters-and-accidents, floods Locations: brisbane-4000, fernvale-4306 Print page ...
We present the draft genome sequence and its analysis for Fusobacterium nucleatum sub spp. vincentii (FNV), and compare that genome with F. nucleatum ATCC 25586 (FN). A total of 441 FNV open reading frames (ORFs) with no orthologs in FN have been identified. Of these, 118 ORFs have no known function and are unique to FNV, whereas 323 ORFs have functional orthologs in other organisms. In addition to the excretion of butyrate, H2S and ammonia-like FN, FNV has the additional capability to excrete lactate and aminobutyrate. Unlike FN, FNV is likely to incorporate galactopyranose, galacturonate, and sialic acid into its O-antigen. It appears to transport ferrous iron by an anaerobic ferrous transporter. Genes for eukaryotic type serine/threonine kinase and phosphatase, transpeptidase E-transglycosylase Pbp1A are found in FNV but not in FN. Unique ABCtransporters, cryptic phages, and three types of ...
The response of Desulfovibrio vulgaris Hildenborough (DvH),a sulphate-reducing bacterium, to nitrate stress was examined usingquantitative proteomic analysis. DvH was stressed with 105 m M sodiumnitrate(NaNO3), a level that caused a 50 percent inhibition in growth.The protein profile of stressed cells was compared with that of cellsgrown in the absence of nitrate using the iTRAQ peptide labellingstrategy and tandem liquid chromatography separation coupled with massspectrometry (quadrupoletime-of-flight) detection. A total of 737 uniqueproteins were identified by two or more peptides, representing 22 percentof the total DvH proteome and spanning every functional category. Theresults indicate that this was a mild stress, as proteins involved incentral metabolism and the sulphate reduction pathway were unperturbed.Proteins involved in the nitrate reduction pathway increased. Increasesseen in transport systems for proline, glycine^ betaineandglutamateindicate that the ...
In 2002 and again in 2003, an investigative journalist unit at ABC News transported a 6.8 kilogram metallic slug of depleted uranium (DU) via shipping container from Istanbul, Turkey to Brooklyn, NY and from Jakarta, Indonesia to Long Beach, CA. Targeted inspection of these shipping containers by Department of Homeland Security (DHS) personnel, included the use of gamma-ray imaging, portal monitors and hand-held radiation detectors, did not uncover the hidden DU. Monte Carlo analysis of the gamma-ray intensity and spectrum of a DU slug and one consisting of highly-enriched uranium (HEU) showed that DU was a proper surrogate for testing the ability of DHS to detect the illicit transport of HEU. Our analysis using MCNP-5 illustrated the ease of fully shielding an HEU sample to avoid detection. The assembly of an Improvised Nuclear Device (IND) -- a crude atomic bomb -- from sub-critical pieces of HEU metal was then examined ...
The uncoupling protein (UCP) is a proton/anion transporter found in the inner mitochondrial membrane of brown adipocyte. Although UCP has nor been detected in mitochondria from any other tissue, it shares structural and catalytic properties with several other mitochondrial carrier proteins. Although UCP was discovered only recently it is one of the most extensively studied mitochondrial carrier proteins.More recently, the mouse, rat, and human genes encoding for UCP have been isolated and sequenced. The availability of these various tools has led to several significant observations. UCP gene expression is strongly controlled at the level of transcription by signals that are activated after the stimulation of brown adipocytes by norepinephrine. The comparison of UCP gene with the genes encoding the adenine nucleotide translocator revealed the existence of structural and evolutionary homologies. Moreover, in humans the UCP gene and one form of ...
Ozsvath and Szabo show that there is a spectral sequence whose E^2 term is the reduced Khovanov homology of L, and which converges to the Heegaard Floer homology of the (orientation reversed) branched double cover of S^3 along L. We prove that the E^k term of this spectral sequence is an invariant of the link L when k>2. If L is a transverse link, then we show that Plamenevskaya's transverse invariant gives rise to a transverse invariant of L in each of these higher terms.
The homologous sequences observed for many calcium binding proteins such as parvalbumin, troponin C, the myosin light chains, and calmodulin has lead to the hypothesis that these proteins have homologous...Full Text Available
CDC7 is an essential gene required for DNA replication in Saccharomyces cerevisiae. Cdc7p homologs have recently been identified in vertebrates, but their role in DNA...Full Text Available
The polymorphic phase behavior of a homologous series of n-saturated 1,2-diacyl phosphatidylethanolamines was investigated by differential scanning calorimetry, 31P-nuclear magnetic resonance, and Fourier...Full Text Available
The term "homology" or "homologous" means an amino acid similarity measured by the program, BLAST (Altschul et al (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:33 89 3402), and expressed as --(% identity n/n). In measuring homology between a peptide and a protein of greater size, homology is measured only in the corresponding region; that is, the protein is regarded as only having the same general length as the peptide, allowing for gaps and insertions.
We iterate Manolescu's unoriented skein exact triangle in knot Floer homology with coefficients in the fraction field of the group ring (Z/2Z)[Z]. The result is a spectral sequence which converges to a stabilized version of delta-graded knot Floer homology. The (E_2,d_2) page of this spectral sequence is an algorithmically computable chain complex expressed in terms of spanning trees, and we show that there are no higher differentials. This gives the first combinatorial spanning tree model for knot Floer homology.
... However blaming vitamin supplements on deaths, or mortality is silly, as the studies are not exactly kosher. Vitamin C in 2,000mg is good for allergies, and niacin (b3) in 10,0000mg is good for depression. This is all examples on how vitamin supplements can help. I call it a security blanket, as food these days is not so good due vitamin and mineral damage due to light, or storage. By the time we eat the apple from the supermarket, there is minimal vitamin and ...
Given a double complex $X$ there are spectral sequences with the $E_2$ terms being either H$_I$ (H$_{II}(X))$ or H$_{II}($H$_I (X))$. But if $H_I(X)=H_{II}(X)=0$ both spectral sequences have all their terms 0. This can happen even though there is nonzero (co)homology of interest associated with $X$. This is frequently the case when dealing with Tate (co)homology. So in this situation the spectral sequences may not give any information about the (co)homology of interest. In this article we give a different way of constructing homology groups of $X$ when H$_I(X)=$H$_{II}(X)=0$. With this result we give a new and elementary proof of balance of Tate homology and cohomology.
Homology analyses of the protein sequences of chicken liver and rat mammary gland fatty acid synthases were carried out. The amino acid sequences of the chicken and rat enzymes are 67% identical. If conservative substitutions are allowed, 78% of the amino acids are matched. A region of low homologies exists between the functional domains, in particular around amino acid residues 1059-1264 of the chicken enzyme. Homologies between the active sites of chicken and rat and of chicken and yeast enzymes have been analyzed by an alignment method. A high degree of homology exists between the active sites of the chicken and rat enzymes. However, the chicken and yeast enzymes show a lower degree of homology. The DADPH-binding dinucleotide folds of the {beta}-ketoacyl reductase and the enoyl reductase sites were identified by comparison with a known consensus sequence for the DADP- and ...
For a word w in the braid group on n-strands, we denote by T_w the corresponding transverse braid in the rotational symmetric tight contact structure on S^3. We exhibit a map on link Floer homology which sends the transverse invariant associated to T_{ws_i} to that associated to T_w, where s_i is one of the standard generators of B_n. This gives rise to a "comultiplication" map on link Floer homology. We use this to generate infinitely many new examples of prime topological link types which are not transversely simple.
It has become increasingly clear that the standard nomenclature for many telencephalic and related brainstem structures of the avian brain is based on flawed once-held assumptions of homology...Full Text Available
1. A high degree of homology in the positions of tyrosine residues in glyceraldehyde 3-phosphate dehydrogenase from lobster and pig muscle, and from yeast, prompted an examination of the reactivity...Full Text Available
Epulopiscium sp. type B is an enormous intestinal symbiont of the surgeonfish Naso tonganus. Intracellular offspring production in Epulopiscium shares features with endospore formation. Here, we characterize the spoIIE homolog in Epulopiscium. The timing of spoIIE gene expression and presence of interacting partners suggest that the activation of ?(F) occurs early in Epulopiscium offspring development. PMID:21398534
We determine the structure of the total homology groups of exotic Springer fibers as affine Weyl group representations. As applications, we provide single top/socle property of standard modules in the exotic Deligne-Langlands correspondence (except for root of unity case), an analogue of Verma's theorem, the coincidence of analytic/geometric gradings in the $C ^{\\infty}$-realization of anti-spherical modules of graded Hecke algebras of type $\\mathsf{BC}$ with unequal parameters, among others.
α1-Fetoprotein transcription factor (FTF), also known as liver receptor homolog 1 (LRH-1) is highly expressed in liver and intestine, where it is implicated in the regulation...Full Text Available
Although thee are descriptions of a range of radioimmunoassays for human prolactin in various biological fluids, only one of these is an homologous assay using human prolactin as the reference standard and tracer as well and an anti-human prolactin antiserum (Sinha, Y.N., Selby, F.W.; Lewis, U.; and Vanderlaan, W.P., 1973, J. Clin. Endocr., Vol. 36, 509). A homologous radioimmunoassay using human putuitary prolactin has been developed. The separation method is based on the double antibody solid phase system. Cross reactivity with human growth hormone (GH), placental lactogen (HPL), the pituitary protein hormones and prolactins of various species were studied as were values found in normal subjects in basal conditions and after a TRH injection. (author).
Cyst nematodes are troublesome parasites that live on, and destroy, a range of important host vegetable plants. Damage caused by the potato cyst nematode has now been reported in over 50 countries. One approach to eliminating the problem is to stimulate early hatching of the nematodes, but key hatching stimuli are not naturally available in sufficient quantities to do so. Here, we report the first chemical synthesis of solanoeclepin A, the key hatch-stimulating substance for potato cyst nematode. The crucial steps in our synthesis are an intramolecular cyclization reaction for construction of the highly strained tricyclo[5.2.1.01,6]decane skeleton (DEF ring system) and an intramolecular Diels???Alder reaction of a furan derivative for the synthesis of the ABC carbon framework. The present ...
The purpose of this study was to compare cardiopulmonary fitness and endurance in 9-11-year-old children with DCD against a group of typically developing children in Taiwan. The Movement ABC test was used to evaluate the motor abilities of children. Forty-one participants (20 children with DCD and 21 children without DCD) were recruited for this study. The cardiopulmonary tests included the 800-m run test and the peak oxygen consumption (peak VO2) test using the Bruce treadmill protocol. No significant differences in age, body height, body weight, body mass index, and percentage of body fat between children with DCD and without DCD were found. However, there were significant differences in the cardiopulmonary endurance tests between children with DCD and without DCD. Children with DCD had ...
Pyogenic sacroiliitis is a rare entity in children. Diagnosis is often delayed because of its variable clinical presentation, low suspicion by the examining physician, and rare findings on radiographs. Delayed diagnosis, however, results in complications such as iliopsoas abscess. We report the case of a 12-year-old girl hospitalized with a 21-day history of fever, pain in the left iliac fossa, and flexion contracture of the hip. On examination, she had fever (38.9 ^oC), psoitis, localized tenderness at the left sacroiliac joint, and pain elicited by lateral compression of the pelvis. The abdominal examination was normal. The erythrocyte sedimentation rate was 130mm in the first hour, C-reactive protein was 186mg/l, and the white blood cell count was 18,400/mm^3, with 79% neutrophils. Urin...
Purpose of ReviewThe vertebrate cap’n’collar family transcription factor Nrf2 and its invertebrate homologs SKN-1 (in worms) and CncC (in flies) function...Full Text Available
DNA double-strand breaks (DSBs) and other lesions occur frequently during cell growth and in meiosis. These are often repaired by homologous recombination (HR). HR may result in the formation of DNA...Full Text Available
The LW blood group antigens reside on a 42-kDa erythrocyte membrane glycoprotein that was purified by immunoaffinity and partially sequenced. From this information, a specific PCR-amplified DNA fragment...Full Text Available
The technology of gene targeting through homologous recombination has been extremely useful for elucidating gene functions in mice. The application of this technology was thought impossible in the large...Full Text Available
The pharmacological effects of a series of fatty acid-choline esters have been studied on the isolated rabbit heart, the isolated guinea-pig ileum and the rat stomach. The effect changed with increasing...Full Text Available
Yeast Sir2 deacetylase is a component of the silent information regulator (SIR) complex encompassing Sir2/Sir3/Sir4. Sir2 is recruited to telomeres through Rap1, and this complex spreads into subtelomeric...Full Text Available
Acetaminophen (N-acetyl-para-aminophenol (APAP), paracetamol) is a commonly used analgesic and antipyretic agent. Although considered safe at therapeutic doses, accidental or intentional...Full Text Available
The standard nomenclature that has been used for many telencephalic and related brainstem structures in birds is based on flawed assumptions of homology to mammals. In particular, the outdated...Full Text Available
The effects of homologous plasma HDL and VHDL fractions on established atherosclerotic lesions were studied in cholesterol-fed rabbits. Atherosclerosis was induced by feeding the animals a 0.5% cholesterol-rich...Full Text Available
URA5 genes encode orotidine-5′-monophosphate pyrophosphorylase (OMPpase), an enzyme involved in pyrimidine biosynthesis. We cloned the Histoplasma capsulatum URA5...Full Text Available
Spectacular increases in the quantity of sequence data genome have facilitated major advances in eukaryotic comparative genomics. By exploiting homology with classical model organisms, this makes possible...Full Text Available
Some cases of autism spectrum disorder (ASD) have mutations in the lipid phosphatase, Pten (phosphatase and tensin homolog on chromosome 10). Tissue...Full Text Available
cdc18+ of Schizosaccharomyces pombe is a periodically expressed gene that is required for entry into S phase and for the coordination of S phase with mitosis. cdc18+ is related to the Saccharomyces...Full Text Available
The fungus Cryptococcus neoformans is a major cause of morbidity and mortality in patients with impaired CD4+ T cell function, particularly those with AIDS. To identify...Full Text Available
RNase BN, the Escherichia coli homolog of RNase Z, was previously shown to act as both a distributive exoribonuclease and an endoribonuclease on model RNA substrates and to be inhibited...Full Text Available
BackgroundGrapevine is subjected to numerous pests and diseases resulting in the use of phytochemicals in large quantities. The will to decrease the use of phytochemicals leads to...Full Text Available
A histidine kinase protein (Cph1) with sequence homology and spectral characteristics very similar to those of the plant phytochrome has been recently identified in the cyanobacterium Synechocystis...Full Text Available
A single large plasmid was isolated from multiplasmid-harboring strains Rhizobium leguminosarum 1001 and R. trifolii 5. These single plasmids, as well as the largest plasmid detectable in R. phaseoli...Full Text Available
The reaction of iodine with glyceraldehyde 3-phosphate dehydrogenase from Bacillus stearothermophilus was investigated. The active-site thiol group of the cysteine residue homologous with cysteine-149...Full Text Available
Juvenile hormone analog (JHA) insecticides are relatively nontoxic to vertebrates and offer effective control of certain insect pests. Recent reports of resistance in whiteflies and mosquitoes demonstrate...Full Text Available
The human immunodeficiency virus type 1 (HIV-1) packages its genomic RNA as a dimer of homologous RNA molecules that has to be selected among a multitude of cellular and viral RNAs. Interestingly, spliced...Full Text Available
Simian retroperitoneal fibromatosis (RF) is a vascular fibroproliferative neoplasm which has many morphological and histological similarities to human Kaposi's sarcoma (KS). Like epidemic KS in AIDS...Full Text Available
The plasmid pE194 (3.7 kilobases) is capable of integrating into the genome of the bacterial host Bacillus subtilis in the absence of the major homology-dependent RecE recombination system. Multiple...Full Text Available
BackgroundDNA repair is the general term for the collection of critical mechanisms which repair many forms of DNA damage such as methylation or ionizing radiation. DNA repair has...Full Text Available
Mitochondria must uptake some phospholipids from the endoplasmic reticulum (ER) for the biogenesis of their membranes. They convert one of these lipids, phosphatidylserine, to phosphatidylethanolamine,...Full Text Available
SUMMARYA gene eam in Clostridium difficile encodes a protein that is homologous to lysine 2,3-aminomutase (LAM) in many other species but does...Full Text Available
Evaluation of the protective efficacy of recombinant T-cell-reactive proteins of Coccidioides posadasii in a murine model of coccidioidomycosis has led to the discovery of potential...Full Text Available
In the Archaea only a handful of ribonucleases involved in RNA processing and degradation have been characterized. One potential group of archaeal ribonucleases are homologues of the bacterial RNase...Full Text Available
SummaryHuman skin expresses elements of the hypothalamo-pituitary-adrenal (HPA) axis including pro-opiomelanocortin (POMC), corticotropin releasing hormone (CRH), the CRH receptor-1...Full Text Available
Homologous to bacteriorhodopsin and even more to proteorhodopsin, xanthorhodopsin is a light-driven proton pump that, in addition to retinal, contains a noncovalently bound carotenoid with a function...Full Text Available
Different tombusviruses were able to support the replication of either homologous or heterologous defective interfering (DI) RNAs, and those infected plants usually developed typical attenuated symptoms....Full Text Available
We have cloned and characterized the entire DNA polymerase gene and flanking regions from Kaposi's sarcoma-associated herpesvirus (KSHV) and two closely related macaque homologs of KSHV, retroperitoneal...Full Text Available
Organisms belonging to the genus Streptomyces produce numerous important secondary metabolites and undergo a sophisticated morphological differentiation program. In many instances these...Full Text Available
The theory of zigzag persistence is a substantial extension of persistent homology, and its development has enabled the investigation of several unexplored avenues in the area of topological data analysis. In this paper, we discuss three applications of zigzag persistence: topological bootstrapping, parameter thresholding, and the comparison of witness complexes.
BmpA, BmpB, BmpC, and BmpD are homologous Borrelia burgdorferi lipoproteins of unknown functions, encoded by the bmp genes of paralogous chromosomal gene family 36....Full Text Available
A search for homologs of the Bacillus subtilis PhoP response regulator in the group A streptococcus (GAS) genome revealed three good candidates. Inactivation of one of these, recently...Full Text Available
Basic fibroblast growth factor (bFGF) exhibits specific binding to the extracellular matrix (ECM) produced by cultured endothelial cells. Binding was saturable as a function both of time and of concentration of {sup 125}I-bFGF. Scatchard analysis of FGF binding revealed the presence of about 1.5 x 10{sup 12} binding sites/mm{sup 2} ECM with an apparent k{sub D} of 610 nM. FGF binds to heparan sulfate (HS) in ECM as evidenced by (i) inhibition of binding in the presence of heparin or HS at 0.1-1 {mu}g/mL, but not by chondroitin sulfate, keratan sulfate, or hyaluronic acid at 10 {mu}g/mL, (ii) lack of binding to ECM pretreated with heparitinase, but not with chondroitinase ABC, and (iii) rapid release of up to 90% of ECM-bound FGF by exposure to heparin, HS, or heparitinase, but not to chondroitin sulfate, keratan sulfate, hyaluronic acid, or chondroitinase ABC. Oligosaccharides derived from depolymerized heparin, and as small as the ...
The authors characterized nine human actin genes that they isolated from a library of cloned human DNA. Measurements of the thermal stability of hybrids formed between each cloned actin gene and ..cap alpha..-, ..beta..-, and ..gamma..-actin mRNA demonstrated that only one of the clones is most homologous to sarcomeric actin mRNA, whereas the remaining eight clones are most homologous to cytoplasmic actin mRNA. By the following criteria they show that these nine clones represent nine different actin gene loci rather than different alleles or different parts of a single gene: (i) the restriction enzyme maps of the coding regions are dissimilar; (ii) each clone contains sufficient coding region to encode all or most of an entire actin gene; and (iii) each clone contains sequences homologous to both the 5' and 3' ends of the coding region of a cloned chicken ..beta..-actin cDNA. They conclude, therefore, that ...
The Drosophila hairy gene encodes a basic helix- loop-helix protein that functions in at least two steps during Drosophila development: (1) during embryogenesis, when it partakes in the establishment of segments, and (2) during the larval stage, when it functions negatively in determining the pattern of sensory bristles on the adult fly. In the rat, a structurally homologous gene (RHL) behaves as an immediate-early gene in its response to growth factors and can, like that in Drosophila, suppress neuronal differentiation events. Here, the authors report the genomic cloning of the human hairy gene homolog (HRY). The coding region of the gene is contained within four exons. The predicted amino acid sequence reveals only four amino acid differences between the human and rat genes. Analysis of the DNA sequence 5[prime] to the coding region reveals a putatitve untranslated exon. To increase the value of the HRY gene as a genetic marker and to assess ...
Enhancing transport sustainability is the password of national, European, and international policies. The development of innovative solutions is ranging from bio fuels to transport analysis models, materials, traction technologies, mobility management, freight villages.
This book describes one approach to building and operating biogas systems. The biogas systems include raw material preparation, digesters, separate gas storage tanks, use of the gas to run engines, and the use of the sludge as fertilizer. Chapters included are: (1) "Introduction"; (2) "Biogas Systems are Small Factories"; (3) "The Raw Materials of Biogas Digestion"; (4) "The Daily Operation of a Biogas Factory"; (5) "The Once a Year Cleaning of the Digester"; (6) "Tanks and Pipes: Storing and Moving Biogas"; (7) "The Factory's Products: Biogas"; (8) "The Factory's Products: Biofertilizer"; (9) "The ABCs of Safety"; and (10) "Conclusion: Profiting from an Appropriate Technology." Many diagrams are provided throughout this handbook. New ideas, composting, bioinsecticides, ferrocement, facts and figures, sources and resources, feasibility studies, problem solving, and vocabulary are presented in the appendices. (YP)
The authors have cloned and determined the entire nucleotide sequence of cDNAs corresponding to the putative {alpha} subunits of the human and rat mast cell high-affinity IgE receptors. Both human and rat cDNAs encode an NH{sub 2}-terminal signal peptide, two immunoglobulin-like extracellular domains (encoded by discrete exons), a hydrophobic transmembrane region, and a positively charged cytoplasmic tail. The human and rat {alpha} subunits share an overall homology with one another and the immunoglobulin gene family, suggesting that they arose from a common ancestral gene and continue to share structural homology with their ligands. In addition, the rat gene is transcribed into at least three distinct forms, each of which yields a somewhat different coding sequence.
The dipteran parasitoids Therobia leonidei and Homotrixa alleni (Tachinidae) use acoustic cues to locate their calling tettigoniid (Ensifera, Orthoptera) hosts. The sexually dimorphic tympanal organs of both fly species are located at the prosternum. For comparison a homologous chordotonal organ in the non-hearing fly Phormia regina, Meigen (Phoridae) is also described. The scolopidial sense organs of the ears have approximately 180 sensory cells in Th. leonidei and 250 cells in H. alleni. Interspecific analysis indicates that the cell number and arrangement might be genus specific in Tachinidae. The mononematic scolopidia, each with one sensory cell, are of different sizes and insert at the tympanal membrane. Large scolopidial units (diameter of sensory cells up to 50??m) extend longitudi...
This report presents a general investigation of the transport requirements associated with the construction and operation of conceptual fusion reactors. Projections of amounts of construction and operating materials requiring transportation are presented for several proposed designs. The material to be shipped is described along with the shipping containers that might be used, the transport modes and the expected impact of transporting these materials. Transportation of both radioactive and nonradioactive materials will be required. Most of these materials are routinely shipped by the transportation industry. Transportation requirements of a representative fusion reactor are also compared with Liquid Metal Fast Breeder Reactor (LMFBR) requirements.
This paper presents a supernetwork equilibrium model integrating supply chain networks with a transport network, namely, a supply chain-transport supernetwork equilibrium model. The model takes into account the behaviour of freight carriers and transport network users to endogenously determine the transport costs generated in the supply chain networks. The interaction between transport network and supply chain networks can also be examined. Results of the numerical tests reveal that the improvement of transport network could enhance the efficiency of supply chain networks. The paper makes contributions to modelling of supply chain networks as well as to that of transport networks.
c-Jun N-terminal kinase 2 (JNK2) isoforms are transcribed from the jnk2 gene and are highly homologous with jnk1 and jnk3 transcriptional products....Full Text Available
Brassica napus is an allotetraploid (AACC) formed from the fusion of two diploid progenitors, Brassica rapa (AA) and Brassica oleracea...Full Text Available
Lethal alleles of the Drosophila k43 gene result in small or missing imaginal discs, greatly reduced mitotic index, and fragmented and abnormally condensed chromosomes. A female-sterile...Full Text Available
Gastrin and its carboxyl-terminal homolog cholecystokinin (CCK) exert a variety of biological actions in the brain and gastrointestinal tract that are mediated in part through one or more G protein-coupled...Full Text Available
The YTH (YT521-B homology) domain was identified by sequence comparison and is found in 174 different proteins expressed in eukaryotes. It is characterized by 14 invariant residues within an α-helix/β-sheet...Full Text Available
RIN proteins serve as guanine nucleotide exchange factors for Rab5a. They are characterized by the presence of a RIN homology domain and a C-terminal Vps9 domain. Currently three family members have...Full Text Available
The nucleotide sequence of 16S rDNA from Euglena gracilis chloroplasts has been determined representing the first complete sequence of an algal chloroplast rRNA gene. The structural part of the 16S...Full Text Available
We show that the relation between D-branes and noncommutative tachyons leads very naturally to the relation between D-branes and K-theory. We also discuss some relations between D-branes and K-homology, provide a noncommutative generalization of the ABS construction, and give a simple physical interpretation of Bott periodicity. In addition, a framework for constructing Neveu--Schwarz fivebranes as noncommutative solitons is proposed.
Several new development trends in the processing of methanol to various chemical products are discussed. The production of acetic acid by carbonylation of methanol will increase. In the future, methanol is expected to represent the raw material in the production of lower alkenes. The synthesis of lower alcohols, of synthetic gas (homologation), and of aromatic hydrocarbons from methanol are in the research stage.
Two sequences with homology to a thioredoxin oligonucleotide probe were detected by Southern blot analysis of Anabaena sp. strain PCC 7120 genomic DNA. One of the sequences was shown to code for a protein...Full Text Available
The major core protein (p28) of MMC-1, an endogenous type C virus of the rhesus monkey (Macaca mulatta), was purified and subjected to structural and immunological analyses. The NH2-terminal amino acid...Full Text Available
Amyloid plaques are a characteristic feature in Alzheimer’s disease (AD). A novel non-toxic contrast agent is presented, Gd-DTPA-K6Aβ1–30, which is homologous to Aβ,...Full Text Available
We have examined homologous fragments of DNA cloned from two different tissues for changes in the dNA sequence which might be related to tissue specific gene expression. The 5' end of the chicken ovalbumin...Full Text Available
The Danish Ministry of Transportation has prepared a national plan of transportation, for the years until 2005. Official policy concerning land, maritime and air transport, personal and cargo transportation, investments and expenditures is outlined. Environmental impacts of energy consumption and air pollution abatement measures are considered. (EG)
The change of the transport coefficient due to the fusion energy source is studied. The scale invariance property of the reduced set of equations is investigated in the presence of the self-heating term due to the fusion reaction. The pressure gradient as well as the fusion power are the free energy sources that dictate the turbulent transport. It is shown that the burning transport coefficient can have a form with much wider variety, and that the transport property could be different owing to the self-heating by the fusion reactions. (author)
Alterations in mass transport patterns of low-density lipoproteins (LDL) and oxygen are known to cause atherosclerosis in larger arteries. We hypothesise that the species transport processes in coronary...Full Text Available
The transport of serine into tobacco (Nicotiana tabacum L. var. Xanthi) cells grown in liquid medium was studied. Serine transport was maximal below pH 4.0. A time-dependent stimulation...Full Text Available
Transport of negative ion beams through plasma is reviewed. The effect of space charge on beam stability and beam emittance is discussed. The approaches to the beam transport problem developed at Los Alamos, Berkeley, Oak Ridge and Culham Laboratory are intercompared. (AIP)
Nutrient transport affinities of nutrient-starved microbial populations were measured as initial slopes of plots of limiting-nutrient transport rates versus extracellular limiting-nutrient concentrations....Full Text Available
Citrate transport via CitS of Klebsiella pneumoniae has been shown to depend on the presence of Na+. This transport system has been expressed in Escherichia coli, and uptake of citrate in E. coli membrane...Full Text Available
To gain insight into ATP-binding cassette transporter A1 (ABCA1) function and its potential role in AD pathology, we analyzed the expression of the cholesterol transporter ABCA1...Full Text Available
Amino acid transport was studied in membrane vesicles of the thermophilic anaerobic bacterium Clostridium fervidus. Neutral, acidic, and basic as well as aromatic amino acids were transported at 40...Full Text Available
Many auxin responses are dependent on redistribution and/or polar transport of indoleacetic acid. Polar transport of auxin can be inhibited through the application of phytotropins such as 1-naphthylphthalamic...Full Text Available
The human zinc finger protein genes (ZFX/Y) were identified as a result of a systematic search for the testis-determining factor gene on the human Y chromosome. Although they play no direct role in sex determination, they are of particular interest because they are highly conserved among mammals, birds, and amphibians and because, in eutherian mammals at least, they have active alleles on both the X and the Y chromosomes outside the pseudoautosomal region. We used in situ hybridization to localize the homologues of the zinc finger protein gene to chromosome 1 of the Australian echidna and to an equivalent position on chromosomes 1 and 2 of the playtpus. The localization to platypus chromosome 1 was confirmed by Southern analysis of a Chinese hamster [times] platypus cell hybrid retaining most of platypus chromosome 1. This localization is consistent with the cytological homology of chromosome 1 between the two species. The zinc finger protein gene homologues were ...
In mammals and insects, paracellular blood barriers isolate the nervous system from the rest of the animal. Glia and accessory cells of the nervous system use pumps, channels, cotransporters, and exchangers collectively to maintain the extracellular ion environment and osmotic balance in the nervous system. At present, the molecular mechanisms that regulate this process remain unclear. In humans, loss of extracellular ion and volume regulation in the nervous system poses serious health threats. Drosophila is a model genetic organism with a proven track record for uncovering molecular mechanisms relevant to human health and disease. Here, we review what is known about extracellular ion and volume regulation in larval abdominal nerves, present some new data about the impact of neural activity on the extracellular environment, and relate the findings to mammalian systems. Homologies have been found at the level of morphology, physiology, molecular mechanisms, and ...
In a holomorphic family $(X_b)_{b\\in B}$ of non-K\\"ahlerian compact manifolds, the holomorphic curves representing a fixed 2-homology class do not form a proper family in general. The deep source of this fundamental difficulty in non-K\\"ahler geometry is the {\\it explosion of the area} phenomenon: the area of a curve $C_b\\subset X_b$ in a fixed 2-homology class can diverge as $b\\to b_0$. This phenomenon occurs frequently in the deformation theory of class VII surfaces. For instance it is well known that any minimal GSS surface $X_0$ is a degeneration of a 1-parameter family of simply blown up primary Hopf surfaces $(X_z)_{z\\in D\\setminus\\{0\\}}$, so one obtains non-proper families of exceptional divisors $E_z\\subset X_z$ whose area diverge as $z\\to 0$. Our main goal is to study in detail this non-properness phenomenon in the case of class VII surfaces. We will prove that, under certain technical assumptions, a lift $\\widetilde E_z$ ...
The chicken extracellular matrix glycoprotein ES/130 is necessary for epithelial-mesenchymal transformation in the developing hear and is also expressed in noncardiac chicken tissues such as limb and notochord. We have identified hES, the human homology of chicken ES/130. Fluorescence in situ hybridization analysis (FISH) localizes hES to human chromosome 20p11.2-p12. FISH analyses of individuals with 20p12 deletions and affected by Alagille syndrome exclude hES as a candidate gene for this disorder. Reverse transcriptase-polymerase chain reaction studies reveal that hES is expressed in both fetal and adult human tissues and that hES expression in the left ventricle is increased in the failing adult heart. Further studies will evaluate how hES mutations may relate to congenital human cardiac and skeletal anomalies as well as cardiac remodeling in the adult. 16 refs., 2 figs.
A mutation to tetracycline sensitivity in a resistant strain of Streptococcus pneumoniae was shown by several criteria to be due to a point mutation in the conjugative o(cat-tet) element found in the chromosomes of strains derived from BM6001, a clinical strain resistant to tetracycline and chloramphenicol. Strains carrying the mutation were transformed back to tetracycline resistance with the high efficiency of a point marker by donor deoxyribonucleic acids from its ancestral strain and from nine other clinical isolates of pneumococcus and by deoxyribonucleic acids from Group D Streptococcus faecalis and Group B Streptococcus agalactiae strains that also carry conjugative tet elements in their chromosomes. It was not transformed to resistance by tet plasmid deoxyribonucleic acids from either gram-negative or gram-positive species, except for one that carried transposon TN916, the conjugative tet element present in the chromosomes of some S. faecalis strains. The results showed that ...
Comparisons were made between various LH and FSH radioimmunoassays currently being used to measure avian hormones. The two LH assays were the homologous chicken system of Follett et al. (1972) and the turkey assay of Burke et al. (1979). These assays were also used in heterologous arrangement by interchanging the iodinated LH fractions and antisera. Five FSH assays were analyzed: two homologous chicken systems (Scanes et al., 1977; Sakai and Ishii, 1980) an assay based on mammalian materials (rat FSH and anti-ovine FSH antiserum, and one using labelled turkey FSH (Burke et al., 1979) with an anti-chicken FSH antiserum. The potencies of purified chicken and turkey gonadotrophin preparations and of a range of plasma samples from Japanese quail were measured in each assay. The two LH systems showed some degree of species specificity, such that chicken LH was more active than turkey LH in the chicken assay, whereas the reverse was true in the ...
This paper shows that a well designed transport system has an embedded exchange value by serving as a market for potential exchange between consumers. Under suitable conditions, one can improve the welfare of consumers in the system simply by allowing some exchange of goods between consumers during transportation without incurring additional transportation costs. We propose an explicit valuation formula to measure this exchange value for a given compatible transport system. This value is always nonnegative and bounded from above. Criteria based on transport structures, preferences and prices are provided to determine the existence of a positive exchange value. Finally, we study a new optimal transport problem with an objective taking into account of both transportation cost and exchange value.
BackgroundWith the exception of vertebrates, most organisms have plasma membrane associated ammonium transporters which primarily serve to import a source of nitrogen for nutritional...Full Text Available
The relative transport capabilities of the cells of the root periphery and cortex were investigated using a variety of experimental techniques. Brief (30 seconds to 1 minute) exposures with the penetrating...Full Text Available
... Marine transport and oil disposal by boats and Matadi pose threats in the region. When the water ... Marine transport and oil disposal by boats and Matadi pose threats in the region. When the water ... ...
We have isolated a genomic clone containing 'Lupinus luteus' 5S ribosomal RNA genes by screening with 5S rDNA probe clones that were hybridized previously with the initiator methionine tRNA preparation (contaminated) with traces of rRNA or its degradation products). The clone isolated contains ten repeat units of 342 bp with 119 bp fragment showing 100% homology to the 5S rRNA from yellow lupine. Sequence analysis indicates only point heterogeneities among the flanking regions of the genes. (author). 6 refs, 3 figs.
A short-chain neurotoxin Pseudechis australis a (toxin Pa a) was isolated from the venom of an Australian elapid snake Pseudechis australis (king brown snake) by sequential chromatography on CM-cellulose, Sephadex G-50 and CM-cellulose columns. Toxin Pa a has an LD50 (intravenous) value of 76 micrograms/kg body wt. in mice and consists of 62 amino acid residues. The amino acid sequence of Pa a shows considerable homology with those of short-chain neurotoxins of elapid snakes, especially of true sea snakes. PMID:4091794
Evaluation of grain boundary composition and structure in superplastically deformed AA5083-based alloys (Al-4.5Mg-1.6Mn-0.2Zr) was carried out in a field-emission gun transmission electron microscope (FEG-TEM). During superplastic deformation at high homologous temperatures materials undergo extensive grain boundary sliding (GBS) which creates a flow of defects in the near-boundary region. Recent literature has shown that the grain-boundary composition in Al-Mg alloys is not necessarily the same as the matrix, and that these differences can have an effect on GBS.
If (S,h) is an open book with disconnected binding then we can form a new open book (S',h') by capping off one of the boundary components of S with a disk. We define a U-equivariant map on Heegaard Floer homology which sends c^+(S',h') to c^+(S,h), and we discuss various applications. In particular, we compute the 3-dimensional invariant associated to any contact structure with non-vanishing contact invariant which is compatible with a genus one open book with periodic monodromy.
The tet repressor regulated expression of the Tn-10-encoded tetracycline resistance determinant in a tetracycline-dependent manner. In the absence of tetracycline, the tet repressor binds as a dimer to the 19-base-pair palindromic tet operator sequence. Amino acid homologies and genetic studies with trans-dominant mutants suggest that sequence-specific recognition of the tet operator involves the extensively studied helix-turn-helix motif. We have used the uracil-DNA glycosylase (UDG) footprinting systems to identify thymine contacts in the tet operator that are essential for the formation of tet repressor-operator complexes.
Four trypsin inhibitor homologs, the first known from Dendroaspis angusticeps venom, were characterized using a combination of gel filtration, cation exchange, reverse-phase liquid chromatography, Edman degradation and mass spectrometry. The four toxins comprise two 57 residue and two 59 residue isoforms. The long toxins possess a Lys-Gln N-terminal extension lacked by the short toxins. The only other structural difference is an Arg/His replacement at position 55. The long Arg55 variant is identical to trypsin inhibitor E from the venom of Dendroaspis polylepis. The name epsilon-dendrotoxin is suggested so as to follow the nomenclature of Benishin, C.G., Sorensen, R.G., Brown, W.E., Krueger, B.K., Blaustein, M.P., 1988. Four polypeptide components of green mamba venom selectively block certain potassium channels in rat brain synaptosomes. Mol. Pharmacol. 34, 152-159. Among snake venom protease inhibitors, the epsilon-dendrotoxins are structurally most like the ...
Homologous and heterologous arterial segments were implanted in Fisher rats subcutaneously for the purpose of examining the antibody titer of the recipients' serum after implantation by means of the immune-adherence hemagglutination method. The antibody titer after implantation both of homologous and heterologous grafts decreased to 1/8 by 2.0 million (M) rads irradiation of high voltage cathode rays. The results suggested that high voltage cathode ray irradiation was not enough for heterologous graft to suppress its tissue reaction. Homografts taken from dogs 3 or 6 hours after sacrifice were irradiated with 2.0 M rads and transplanted in canine carotid artery using the technic of end-to-end anastomosis. Angiograms 6 months after operation revealed excellent patency rate in all the grafts of 28 dogs. furthermore, findings of the grafts from 1 week to 5 years after operation on scanning and transmission electron microscopies were ...
Homologous and heterologous arterial segments were implanted in Fisher rats subcutaneously for the purpose of examining the antibody titer of the recipients' serum after implantation by means of the immune-adherence hemagglutination method. The antibody titer after implantation both of homologous and heterologous grafts decreased to 1/8 by 2.0 million (M) rads irradiation of high voltage cathode rays. The results suggested that high voltage cathode ray irradiation was not enough for heterologous graft to suppress its tissue reaction. Homografts taken from dogs 3 or 6 hours after sacrifice were irradiated with 2.0 M rads and transplanted in canine carotid artery using the technic of end-to-end anastomosis. Angiograms 6 months after operation revealed excellent patency rate in all the grafts of 28 dogs. furthermore, findings of the grafts from 1 week to 5 years after operation on scanning and transmission electron microscopies were evaluated. ...
The sequencing and detailed comparative functional analysis of genomes of a number of select botanical models open new doors into comparative genomics among the angiosperms, with potential benefits for improvement of many orphan crops that feed large populations. In this study, a set of simple sequence repeat (SSR) markers was developed by mining the expressed sequence tag (EST) database of sorghum. Among the SSR-containing sequences, only those sharing considerable homology with rice genomic sequences across the lengths of the 12 rice chromosomes were selected. Thus, 600 SSR-containing sorghum EST sequences (50 homologous sequences on each of the 12 rice chromosomes) were selected, with the intention of providing coverage for corresponding homologous regions of the sorghum genome. Primer pairs were designed and polymorphism detection ability was assessed using parental pairs of two existing sorghum mapping populations. ...
A method for the introduction of N-(/sup 3/H)acetyl groups into glycosaminoglycans is described. The procedure is based on (/sup 3/H)acetylation of N-unsubstituted hexosamine residues by treating the polysaccharides with (/sup 3/H)acetic anhydride. Preparations of heparin and heparin sulfate were found to contain significant numbers of N-unsubstituted hexosamine residues, as isolates. In contrast, such units could not be detected in chondroitin sulfate, dermatan sulfate, or hyaluronic acid. These polysaccharides were therefore subjected to partial N-deacetylation by reaction with hydrazine in the presence of hydrazine sulfate. After treatment with (/sup 3/H)acetic anhydride, the specific activities of the resulting labeled polysaccharide preparations ranged between 0.1 X 10/sup 6/ and 0.6 X 10/sup 6/ cpm /sup 3/H/..mu..g of uronic acid. The /sup 3/H-labeled polysaccharide preparations did not differ significantly from the corresponding unlabeled starting materials with regard to ...
BACKGROUND: Denys-Drash Syndrome (DDS) is an uncommon disorder that appears sporadically and in rare cases may be inherited as an autosomal dominant trait It manifests either at birth or within the first year of life and typically consists of the triad of congenital nephropathy, Wilms tumour and intersex disorder. CASE REPORT: A 10 year-old Caucasian girl was referred to the Dental Department, at Glasgow Royal Hospital for Sick Children by her Paediatric Nephrologist Consultant. The patient was being teased by her peers over her markedly discoloured teeth. The dental history revealed that the patient was a regular dental attendee from an early age. She was dentally anxious having only experienced dental treatment under general anaesthesia (GA) when she was 4 years old. Apparently her primary dentition also showed a generalised discolouration. TREATMENT: This consisted of multiple visits for diet analysis and tooth brushing instruction with the use of disclosing tablets. Plaque control ...
A method to deduce the 2 x 2 transport matrix for coupled heat and particle transport in Tokamaks is proposed. The method applies to perturbative experiments, and is based on a Fourier transform of the measured signals of temperature and density. By analyzing different linear combinations of temperature and density, the eigenvectors and eigenvalues of the transport matrix are determined. The method is tested for a number of illustrative cases using simulated data, and the sensitivity to noise on the signals is evaluated. (author).
Development of the engineering technology basis of pyrometallurgical reprocessing is a key issue for industrialization. For development of the transport technologies of molten salt and liquid cadmium at around 773 K, a salt transport test rig and a metal transport test rig were newly installed in an Ar glove box. Function of the salt transport test rig was confirmed with LiCl-KCl molten salt, and the transport behaviour of molten salt was found to follow that of water. The molten salt/liquid metal contactor for Ln/An separation was newly designed and installed. The test with a single-stage contactor was successful with simulated elements, and a three-stage contactor is now under development. A large-scale electro-refiner with a function to transport molten salt and liquid cadmium were newly designed, and to be installed for demonstration test with simulated ...
In drug development, Caco-2 cells are often employed to study the influence of membrane transporters on drug permeability. The aim of the current study was to characterize permeability and kinetic parameters of selected organic anionic compounds in Caco-2 cells, and to investigate whether the Caco-2 cell line may be used as an overall model to predict interactions on multiple membrane transporters in the intestine. Taurocholic acid (TCA) and estrone-3-sulfate (E(1) S) were used as model substrates. Possible inhibitors studied were TCA, E(1) S, taurolithocholic acid, fluvastatin, and glipizide. The effects of these compounds on initial uptake, apparent permeability, and intracellular end-point accumulations of the probe substrates were studied. Both interactions on apical and basolateral influx transporters were observed. These interactions were proposed to be mediated mainly by the apical sodium-dependent bile acid ...
The survey of radiation protection programmes for transport has been jointly performed by three scientific organisations I.P.S.N. (France), G.R.S. ( Germany), and N.R.P.B. (United kingdom) on behalf of the European Commission and the pertaining documentation summarises the findings and conclusions of the work that was undertaken with the principal objectives to provide guidance on the establishment, implementation and application of radiation protection programmes for the transport of radioactive materials by operators and the assessment and evaluation of such programmes by the competent authority and to review currently existing radiation protection programmes for the transport of radioactive materials. (N.C.)
Solenoid transport of high current, heavy ion beams is considered for several stages of a heavy ion fusion driver. In general this option is more efficient than magnetic quadrupole transport at sufficiently low kinetic energy and/or large e/m, and for this reason it has been employed in electron induction linacs. Ideally an ion beam would be transported in a state of Brillouin flow, i.e. cold in the transverse plane and spinning at one half the cyclotron frequency. The design of appropriate solenoids and the equilibrium and stability of transported ion beams are discussed. An outline of application to a fusion driver is also presented.
A stochastic-convective reactive (SCR) transport method is developed for one-dimensional steady transport in physically heterogeneous media with nonlinear degradation. The method is free of perturbation amplitude limitations and circumvents the difficulty of scale dependence of phenomenological parameters by avoiding volume-averaged specifications of diffusive/dispersive fluxes. The transport system is conceptualized as an ensemble of independent convective-reactive streamlines, each characterized by a randomized convective velocity (or travel time). Dispersive effects are treated as a component of the randomness in the streamline velocity ensemble, so no explicit expression for hydrodynamic dispersive flux is written in the streamline transport equation. The expected value of the transport over the stream tube ensemble is obtained as an average of solutions to the reactive ...
The interfacial area transport equation dynamically models two-phase flow regime transitions and predicts continuous changes of the interfacial area concentration along the flow field. It replaces the flow regime-dependent correlations for the interfacial area concentration in thermal-hydraulic system analysis. In the present study, detailed formulation of the interfacial area transport equation is presented along with its evaluation results based on the detailed benchmark experiments. In view of model evaluation, the equation is simplified into one-dimensional steady state one-group interfacial area transport equation. The prediction made by model agrees well with the experimental data obtained in round pipes of various diameters. The framework for the two-group transport equation and the necessary constitutive relations are also presented in view of bubble transport of various ...
Kawasaki Steel started the construction of an automatic steel strip coil transportation system in the 1980's. Presently, most of the in-plant transportation of hot-rolled or cold-rolled coils are automated. For establishing the automatic transportation, there were developed various technologies, such as, the optimum handling technology using artificial intelligence, the technology of preventing additional piling of coils on existing coils by using an ultrasonic wave and a laser beam. Through the improvements of these technologies, a full-automated transportation system has been achieved and the system has contributed substantially toward product quality assurance and cost reduction. This paper gives the changes in the automation technology of coil transportation in the automation of No. 3 finishing hot rolling mill, now having the latest automatic system, in Chiba Works. ...
We study contact structures compatible with genus one open book decompositions with one boundary component. Any monodromy for such an open book can be written as a product of Dehn twists around dual non-separating curves in the once-punctured torus. Given such a product, we supply an algorithm to determine whether the corresponding contact structure is tight or overtwisted when the monodromy is pseudo-Anosov. We rely on Ozsv{\\'a}th-Szab{\\'o} Heegaard Floer homology in our construction and, in particular, we completely identify the $L$-spaces with genus one, one boundary component, pseudo-Anosov open book decompositions. Lastly, we reveal a new infinite family of hyperbolic three-manifolds with no co-orientable taut foliations, extending the family discovered in \\cite{RSS}.
We address the deviations of the scaling relations of elliptical galaxies from the expectations based on the virial theorem and homology, including the "tilt" of the "fundamental plane" and the steep decline of density with mass. We show that such tilts result from dissipative major mergers once the gas fraction available for dissipation declines with progenitor mass, and derive the scaling properties of the progenitors. We use hydrodynamical simulations to quantify the effects of major mergers with different gas fractions on the structural properties of galaxies. The tilts are driven by the differential shrinkage of the effective stellar radius as a function of dissipation in the merger, while the correlated smaller enhancements in internal velocity and stellar mass keep the slope of the velocity-stellar mass relation near V \\pr M_*^{1/4}. The progenitors match a straightforward model of disc formation in LCDM haloes. Their total to stellar mass ratio within the ...
We introduce a weak order ideal property that suffices for establishing the Evans-Griffith Syzygy Theorem. We study this weak order ideal property in settings that allow for comparison between homological algebra over a local ring R versus a hypersurface ring R =R/(x^n). Consequently we solve some relevant cases of the Evans-Griffith syzygy conjecture over local rings of unramified mixed characteristic p, with the case of syzygies of prime ideals of Cohen-Macaulay local rings of unramified mixed characteristic being noted. We reduce the remaining considerations to modules annihilated by p^s, s>0, that have finite projective dimension over a hypersurface ring.
Fifteen quantitative bone scintigraphies were performed in an adolescent girl during the follow-up of a femoral osteogenic sarcoma treated by chemotherapy and massive allograft. Three hours after injection of the radiopharmaceutical (7.4 MBq/kg of 99mTc-MDP) bone activity was measured in the inferior limbs at several regions of interest centered on the hips, femurs (proximal, middle, distal) and proximal tibias. The variations of relative bone activities A/S (ratio of corresponding counting rates between two homologous regions in the affected A and in the healthy S limb) and of absolute bone activities (expressed in counts/pixel-second) are interpreted as a function of times during treatment. The quantitative results are discussed with regard to main phenomena influencing bone activity in this particular clinical case: bone growth, chemotherapy and neo-osteogenesis in allograft.
Each of the three cysteine residues in the Escherichia coli RecA protein was replaced with a number of other amino acids. To do this, each cysteine codon was first converted to a chain-terminating amber codon by oligonucleotide-directed mutagenesis. These amber mutants were then either assayed for function in different suppressor strains or reverted by a second round of mutagenesis with oligonucleotides that had random sequences at the amber codon. Thirty-three different amino acid substitutions were obtained. Mutants were tested for three functions of RecA: survival following UV irradiation, homologous recombination, and induction of the SOS response. It was found that although none of the cysteines is essential for activity, mutations at each of these positions can affect one or more of the activities of RecA, depending on the particular amino acid substitution. In addition, the cysteine at position 116 appears to be involved in the RecA-promoted cleavage of the ...
Complex I (NADH:ubiquinone oxidoreductase) is the largest enzyme of the mitochondrial respiratory chain. Compared to its bacterial counterpart which encompasses 14-17 subunits, mitochondrial complex I has almost tripled its subunit composition during evolution of eukaryotes, by recruitment of so-called accessory subunits, part of them being specific to distinct evolutionary lineages. The increasing availability of numerous broadly sampled eukaryotic genomes now enables the reconstruction of the evolutionary history of this large protein complex. Here, a combination of profile-based sequence comparisons and basic structural properties analyses at the protein level enabled to pinpoint homology relationships between complex I subunits from fungi, mammals or green plants, previously identified...
The response regulator protein is a core element of two-component signaling pathway. In this study, we investigated functions of BRRG-1 of Botrytis cinerea, a gene that encodes a putative response regulator protein, which is homologous to Rrg-1 in Neurospora crassa. The BRRG-1 gene deletion mutant ?Brrg1-62 was unable to produce conidia. The mutant showed increased sensitivity to osmotic stress mediated by NaCl and KCl, and to oxidative stress generated by H2O2. Additionally, the mutant was more sensitive to the fungicides iprodione, fludioxonil, and triadimefon than the parental strain. Western-blot analysis showed that the Bos-2 protein, the putative downstream component of Brrg-1, was not phosphorylated in the ?Brrg1-62. Real-time polymerase chain reaction assays showed that expression ...
The similarity in the genetic regulation of arthropod and vertebrate appendage formation has been interpreted as the product of a plesiomorphic gene network that was primitively involved in bilaterian appendage development and co-opted to build appendages (in modern phyla) that are not historically related as structures. Data from lophotrochozoans are needed to clarify the pervasiveness of plesiomorphic appendage-forming mechanisms. We assayed the expression of three arthropod and vertebrate limb gene orthologs, Distal-less (Dll), dachshund (dac), and optomotor blind (omb), in direct-developing juveniles of the polychaete Neanthes arenaceodentata. Parapodial Dll expression marks pre-morphogenetic notopodia and neuropodia, becoming restricted to the bases of notopodial cirri and to ventral ...
The dependence was determined of the decontamination factor ratio found in the presence of a carrier and in a carrier-free system on carrier concentration at different concentrations of a complexing agent in a solution. Bearing balls were used as contamination materials while a mixture of "1"5"2Eu and "1"5"4Eu isotopes was used as a contaminant, citric acid in a concentration of 5x10"-"3 to 1x10"-"1 mol/dm"3 as a complexing agent, and Eu, lanthanum and aluminium at a concentration range of 1x10"-"5 to 1x10"-"2 mol/dm"3 as carriers. While no increase in the decontamination factor was found for aluminium, a considerable increase was observed in the isotopic and homologic carriers and the concentration dependence of the carrier reached the maximum. An equation was derived explaining the effect by the isotope exchange between the contaminant and the carrier and by the reaction between the carrier and the complexing agent. (J.F.).
Withania somnifera (L.) Dunal (Family, Solanaceae), commonly known as Ashwagandha is one of the most valuable medicinal plants synthesizing large number of pharmacologically active secondary metabolites known as withanolides. Though the plant has been well characterized in terms of phytochemical profiles as well as pharmaceutical activities, not much is known about the genes responsible for biosynthesis of these compounds. In this study, we have characterized a gene encoding farnesyl diphosphate synthase (FPPS; EC 2.5.1.10), a key enzyme in the pathway of biosynthesis of isoprenoids, from W. somnifera. The full-length cDNA of Withania somnifera FPPS (WsFPPS) of 1,253?bps encodes a polypeptide of 343 amino acids. The amino acid sequence homology and phylogenetic analysis suggest that WsFPPS...
Abstract NorUDCA (24 norursodeoxycholic acid), the C23 homolog of ursodeoxycholic acid (UDCA), showed remarkable therapeutic effects in cholestatic Mdr2 (Abcb4) (multidrug resistance protein 2/ATP binding cassette b4) knockout mice with sclerosing/fibrosing cholangitis. In contrast to UDCA, norUDCA is inefficiently conjugated in human and rodent liver, and conjugation has been discussed as a key step for the anticholestatic action of UDCA in cholestasis. We compared the choleretic, anticholestatic, and antiapoptotic properties of unconjugated and taurine conjugated UDCA (C24) and norUDCA (C23) in isolated perfused rat liver (IPRL) and in natrium/taurocholate cotransporting polypeptide (Ntcp) transfected human hepatoma (HepG2) cells. Taurolithocholic acid (TLCA) was used to induce a predomi...
Excessive physical activity plays an important role in the progression of anorexia nervosa (AN) by accelerating weight loss during dietary restriction. To search for mechanisms underlying this trait, a panel of mouse chromosome substitution strains derived from C57BL/6J and A/J strains was exposed to a scheduled feeding paradigm and to voluntary running wheel (RW) access. Here, we showed that A/J chromosomes 4, 12 and 13 contribute to the development of a disrupted RW activity in response to daily restricted feeding. This pattern is characterized by intense RW activity during the habitual rest phase and leads to accelerated body weight loss. Regions on mouse chromosomes 4, 12 and 13 display homology with regions on human chromosomes linked with anxiety and obsessionality in AN cohorts. The...
To study interactions between plants and plant-parasitic nematodes, several omics studies have nowadays become extremely useful. Since most data available so far is derived from sedentary nematodes, we decided to improve the knowledge on migratory nematodes by studying the transcriptome of the nematode Pratylenchus coffeae through generating expressed sequence tags (ESTs) on a 454 sequencing platform. In this manuscript we present the generation, assembly and annotation of over 325,000 reads from P. coffeae. After assembling these reads, 56,325 contigs and singletons with an average length of 353bp were selected for further analyses. Homology searches revealed that 25% of these sequences had significant matches to the Swiss-prot/trEMBL database and 29% had significant matches in nematode E...
Amphiphysin is a protein concentrated in neuronal synapses and peripherally associated with neurotransmitter vesicles. It is expressed in many neurons of the central and peripheral nervous systems, in the adrenal medulla, in the anterior and posterior pituitary, in cell lines of the endocrine pancreas, and in spermatocytes. Its subcellular localization and tissue distribution indicate a potential involvement in mechanisms of regulated exocytosis. A role in the dynamic organization of the membrane-associated cytoskeleton is suggested by structural homology to the products of two yeast genes, RVS161 and RVS167, whose mutation results in an abnormal actin distribution, disturbs budding morphology, and impairs cell entry into stationary phase. Limited stretches of sequence similarity, including an SH3 domain, are also shared with other actin-binding proteins. Amphiphysin is the dominant autoantigen in paraneoplastic Stiff-Man syndrome, a neurological autoimmune ...
Uridine phosphorylase (UPP) catalyzes the reversible conversion of uridine to uracil and ribose-1-phosphate and plays an important pharmacological role in activating fluoropyrimidine nucleoside chemotherapeutic agents such as 5-fluorouracil and capecitabine. Most vertebrate animals, including humans, possess two homologs of this enzyme (UPP1 & UPP2), of which UPP1 has been more thoroughly studied and is better characterized. Here, we report two crystallographic structures of human UPP2 (hUPP2) in distinctly active and inactive conformations. These structures reveal that a conditional intramolecular disulfide bridge can form within the protein that dislocates a critical phosphate-coordinating arginine residue (R100) away from the active site, disabling the enzyme. In vitro activity measurem...
A carrier-free radioiodinated phenylazide derivative of forskolin, 3-iodo-4-azidophenethylamido-7-O-succinyl-deacetyl-forskolin (({sup 125}I)IAPS-forskolin), has been shown to be a highly selective photoaffinity probe for the human erythrocyte glucose transported and the glucose transport proteins found in several mammalian tissues and cultured cells where the glucose transport protein is present at a low concentration. The photoincorporation of ({sup 125}I)IAPS-forskolin into these glucose transporters was blocked by D- (but not L-) glucose, cytochalasin B, and forskolin. In addition to labeling the mammalian glucose transport proteins, ({sup 125}I)IAPS-forskolin also labeled the L-arabinose transporter from E. coli. In muscle and adipose tissues, glucose transport is markedly increased in response to insulin. ({sup 125}I)IAPS-forskolin was ...
A carrier-free radioiodinated phenylazide derivative of forskolin, 3-iodo-4-azidophenethylamido-7-O-succinyl-deacetyl-forskolin (["1"2"5I]IAPS-forskolin), has been shown to be a highly selective photoaffinity probe for the human erythrocyte glucose transported and the glucose transport proteins found in several mammalian tissues and cultured cells where the glucose transport protein is present at a low concentration. The photoincorporation of ["1"2"5I]IAPS-forskolin into these glucose transporters was blocked by D- (but not L-) glucose, cytochalasin B, and forskolin. In addition to labeling the mammalian glucose transport proteins, ["1"2"5I]IAPS-forskolin also labeled the L-arabinose transporter from E. coli. In muscle and adipose tissues, glucose transport is markedly increased in response to insulin. ["1"2"5I]IAPS-forskolin was shown to ...
BackgroundThe equal headway instability phenomenon is pervasive in public transport systems. This instability is characterized by an aggregation of vehicles that causes inefficient...Full Text Available
The Transportation Energy Data Book: Edition 12 is a statistical compendium prepared and published by Oak Ridge National Laboratory under contract with the Office of Transportation Technologies in the Department of Energy. Designed for use as a desk-top reference, the data book represents an assembly and display of statistics and information that characterize transportation activity, and presents data on other factors that influence transportation energy use. The purpose of this document is to present relevant statistical data in the form of tables and graphs. Each of the major transportation modes--highway, air, water, rail, pipeline--is treated in separate chapters or sections. Chapter 1 compares US transportation data with data from seven other countries. Aggregate energy use and energy supply data for all modes are presented in Chapter 2. The highway mode, ...
We have investigated the transport of tritiated indole-3-acetic acid (IAA) in intact, red light-grown maize (Zea mays) coleoptiles during gravitropic induction and the subsequent development...Full Text Available
The transport of arginine into isolated barley (Hordeum vulgare L.) mesophyll vacuoles was investigated. In the absence of ATP, arginine uptake was saturable with a Km...Full Text Available
1. The short duration cerebrovascular perfusion method for measuring permeability of the blood-brain barrier has been adapted to measuring transport into the retina. 2. The method has been characterized...Full Text Available
Three flocks raised for broiler or roaster performance tests were studied to determine the incidence and sources of salmonellae during the growing period, transport and processing and to relate these...Full Text Available
Irritable bowel syndrome is a frequent gastrointestinal disorder of unknown etiology. The serotonin transporter regulates the intensity and duration of serotonin signaling in the gut and is,...Full Text Available
Amino acid transport in membrane vesicles of Bacillus stearothermophilus was studied. A relatively high concentration of sodium ions is needed for uptake of L-alanine (Kt = 1.0 mM) and L-leucine (Kt...Full Text Available
The length polymorphism of the serotonin (5-HT) transporter gene promoter region has been implicated in altered 5-HT function and, in turn, neuropsychiatric illnesses, such as anxiety and depression....Full Text Available
The rate-limiting step in the transit of dietary fat across the intestinal absorptive cell is its exit from the endoplasmic reticulum (ER) in a specialized ER-to-Golgi transport vesicle, the prechylomicron...Full Text Available
The DUR3 gene, which encodes a component required for active transport of urea in Saccharomyces cerevisiae, has been isolated, and its sequence has been determined. The deduced DUR3 protein profile...Full Text Available
We investigated the effect of irradiation on the kinetic characteristics of amino acid and glucose transport, and the effect on the activity of the cell membrane-bound enzyme 5'-nucleotidase and on the receptor-mediated stimulation of cyclic adenosine monophosphate synthesis by prostaglandin E1. Irradiation inhibited the sodium-dependent amino acid transport by a reduced binding of the amino acid to the transport unit. The transport of glucose, which appeared to be a sodium-independent process, was temporarily stimulated by increased maximal velocity of the transport. No effect was found on the binding to the transport unit. Irradiation increased the 5'-nucleotidase activity and decreased the prostaglandin E1-stimulated cyclic adenosine monophosphate synthesis 48 h after exposure to 20 Gy. It is concluded that irradiation decreases ...
Geological clay formations are investigated for use as final underground deposit for heat producing nuclear waste. Special kinds of clay (e.g. bentonite) can also be used for the construction of geotechnical barriers. For the long time safety prognosis of the nuclear waste repositories the development of geochemical transport models is indispensable. The transport of aqueous solutions in clay is a complex process. The three-layer-minerals bentonite and illite swell by the adsorption of water, if the volume is restricted a high swelling pressure develops. The excellent barrier effect of natural clay formations and geotechnical clay barriers is based on the high swelling pressure and the high adsorption capacity for radionuclides and other pollutants. The two-layer-mineral kaolinite has no swelling capacity. In contrast to sandy layers a special geochemical transport potential exists in clay besides the well known matrix ...
Three homeologous genes encoding a sucrose (Suc) transporter (SUT) in hexaploid wheat (Triticum aestivum), TaSUT1A, 1B, and 1D, were...Full Text Available
A rapid filtration technique has been used to measure at room temperature the kinetics of ADP and ATP transport in rat heart mitochondria in the millisecond time range. Transport was stopped by cessation of the nucleotide supply, without the use of a transport inhibitor, thus avoiding any quenching delay. The kinetics of ({sup 14}C)ADP transport in energized mitochondria were apparently monophasic. The rate of transport of ({sup 14}C)ATP in energized mitochondria was 5-10 times lower than that of ({sup 14}C)ADP. Upon uncoupling, the rate of ({sup 14}C)ATP uptake was enhanced, and that of ({sup 14}C)ADP uptake was decreased. However, the two rates did not equalize, indicating that transport was not exclusively electrogenic. Transport of ({sup 14}C)ADP and ({sup 14}C)ATP by resting mitochondria followed biphasic kinetics. Depletion of ...
We studied multi-joint coordination during tasks of transporting real and imaginary objects with two arms. One of the arms was unexpectedly arrested in one third of trials performed. In the...Full Text Available
This study addresses the mechanism of transport of the H2-receptor antagonist, cimetidine, by the human placenta. A 4-h recycling perfusion of a single placental cotyledon of normal, term, human placenta...Full Text Available
Hereditary hypouricemia may result from mutations in the renal tubular uric acid transporter URAT1. Whether mutation of other uric acid transporters produces a similar phenotype is unknown. We studied...Full Text Available
We analyze transport through conical channels due to the difference in particle concentration on the two sides of the membrane. Because of the detailed balance, fluxes of non-interacting particles...Full Text Available
The interfacial area transport equation dynamically models the two-phase flow regime transitions and predicts continuous change of the interfacial area concentration along the flow field. Hence, when employed in the numerical thermal-hydraulic system analysis codes, it eliminates artificial bifurcations stemming from the use of the static flow regime transition criteria. Accounting for the substantial differences in the transport phenomena of various sizes of bubbles, the two-group interfacial area transport equations have been developed. The group 1 equation describes the transport of small-dispersed bubbles that are either distorted or spherical in shapes, and the group 2 equation describes the transport of large cap, slug or churn-turbulent bubbles. The source and sink terms in the right-hand-side of the transport equations have been established by ...
ObjectivesThe expected result of the proposed research will be a complete technique for taking the decision maker through the complex process of determining what type types of flexibly delivered public transport service - if any - suits their requirements, and what service characteristics are best adapted to the constraints of their operating environment. To summarise, the objectives are to:~%~ Investigate the various factors which influence the development of an appropriate public transport system for ar [continued...]DescriptionCurrently, many statutory authorities and public transport operators are experimenting with or considering flexibly delivered public transport systems, now generally referred to as Demand Responsive Transport (DRT) services, mainly with a view to improving social inclusion in rural and urban areas that are difficult to cover by conventional public ...
A balanced application of chemiosmosis to plant mitochondria is presented. Electron transport carriers are sequenced to achieve proton translocation across the inner membrane. The processes of proton translocation, use, and release are integrated to yield a description of the effective proton gradient available for metabolite transport and ATP synthesis. 27 references, 6 figures.
Amino acid transport was studied in membranes of the peptidolytic, thermophilic, anaerobic bacterium Clostridium fervidus. Uptake of the negatively charged amino acid L-glutamate, the neutral amino...Full Text Available
In the safety assessment of a potential repository for spent nuclear fuel, it is important to assess the consequences of a hypothetical leak of radionuclides through the seabed and into a waterborne transport phase. Radionuclides adsorbed to sediment particles may be transported great distances through the processes of sedimentation and resuspension. This study investigates the transport patterns of sediment particles of two different sizes, released in the Forsmark and Laxemar area. The results show that the closed waters around Forsmark to a higher degree makes the particles stay in the area close to the release points
... operation, experimental data reduction, and application of PES results to studies related to estuarine and coastal sediment transport problems are ...
The radiative transport problem is cast in integral form using a transport kernel. The transport kernel has an explicit representation in terms of a Feynman Path Integral over all paths between selected points in a volume. This representation is setup in detail. Numerical evaluation of this Path Integral is formulated with a Frenet-Serret based procedure for generating valid random paths, and with a numerical evaluation of the weight for each valid path. Very early sanity checks of a numerical implementation are reported. Approaches to optimization are identified. (authors)
A low power polychromatic beam of microwaves is used to diagnose the behavior of turbulent fluctuations in the core of the JT-60U tokamak during the evolution of the internal transport barrier. A continuous reduction in the size of turbulent structures is observed concomitant with the reduction of the density scale length during the evolution of the internal transport barrier. The density correlation length decreases to the order of the ion gyroradius, in contrast to the much longer scale lengths observed earlier in the discharge, while the density fluctuation level remain similar to the level before transport barrier formation.
The requirements for transporting high-current, negative-ion beams are presented with particular emphasis on applications involving negative-hydrogen-ion beams. In addition to the usual matching and steering problems, particular attention must be paid to beam emittance growth in the transport system. Depending on the application, a number of approaches have been developed using both magnetic and electric lenses. I discuss the design considerations for transporting and matching these beams to radiofrequency quadrupole accelerators, and present a survey of the various types of beamlines now used for negative-ion beams.
The requirements for transporting high-current, negative-ion beams are presented with particular emphasis on applications involving negative-hydrogen-ion beams. In addition to the usual matching and steering problems, particular attention must be paid to beam emittance growth in the transport system. Depending on the application, a number of approaches have been developed using both magnetic and electric lenses. I discuss the design considerations for transporting and matching these beams to radio-frequency quadrupole accelerators, and present a survey of the various types of beamlines now used for negative-ion beams.
The requirements for transporting high-current, negative-ion beams are presented with particular emphasis on applications involving negative-hydrogen-ion beams. In addition to the usual matching and steering problems, particular attention must be paid to beam emittance growth in the transport system. Depending on the application, a number of approaches have been developed using both magnetic and electric lenses. I discuss the design considerations for transporting and matching these beams to radiofrequency quadrupole accelerators, and present a survey of the various types of beamlines now used for negative-ion beams.
... POVs) overseas when space was available. ... information management system launched in 2004. ... Personally Procured Transportation (PPT) system ...
Information that describes the use of the Otto cycle engines in transportation is summarized. The transportation modes discussed in this report include the following: automobiles, light trucks, heavy trucks, marine, recreational vehicles, motorcycles, buses, aircraft, and snowmobiles. These modes account for nearly 100% of the gasoline and LPG consumed in transportation engines. The information provided on each of these modes includes descriptions of the average energy conversion efficiency of the engine, the capital stock, the amount of energy used, and the activity level as measured in ton-miles. Estimates are provided for the years 1980 and 2000.
A brief review is presented of transport in the boundary region of magnetized plasmas by blob-like filaments. Such structures have enhanced levels of particles and heat, are elongated along the magnetic field lines and are localized in the drift plane across the field. The motion of an isolated blob structure is described in some detail and the contribution of such filaments to turbulence-driven transport are discussed. Results are presented from numerical simulations and probe measurements in tokamak plasmas. An interpretation is given of the measured dependence of particle density and transport on experimental control parameters in the scrape-off layer. (author)
The analysis of plasma density oscillations and ExB rotation of U-3M torsatron plasma was performed by UHR correlation reflectometry during the transport barrier formation. The connections between these characteristics and the phenomenon of inner and edge transport barrier formation were determined experimentally at the different values of HF power and plasma density.
The effect of open pit mine motor transport with ICE on the air basin is examined using the example of shale mines and open pits of the production association ''Estonslanets''. Efficient areas of use of different types of open pit mine motor transport, diesel-trolley tracks and electric cars are determined based on technical-economic and ecologic feasibility.
The transport of Rn through activated charcoal was modelled for a charcoal canister used for surface emanation measurements. Derived Rn concentration distributions in full and half charcoal charges were compared with measured Rn profiles. The distributions were also compared with an empirical expression for the measured profiles. Close agreement was observed between the measured, empirically generated and derived profiles. The influence of temperature and humidity on Rn accumulation and transport is also discussed.
Within the EU-Project VOTALP (Vertical Ozone Transports in the Alps), we have shown that deep alpine valleys like the Mesolcina Valley very efficiently transport air out of the polluted valley up to altitudes between 2000 and near 4000 m asl (above sea level). Pollutants emitted in these valleys are very efficiently transported up to high altitudes. (author) 2 figs., 1 tab., 2 refs.
The effect of 10 wk endurance swim training on 3-O-methylglucose (3-MG) uptake (at 40 mM 3-MG) in skeletal muscle was studied in the perfused rat hindquarter. Training resulted in an increase of approximately 33% for maximum insulin-stimulated 3-MG transport in fast-twitch red fibers and an increase of approximately 33% for contraction-stimulated transport in slow-twitch red fibers compared with nonexercised sedentary muscle. A fully additive effect of insulin and contractions was observed both in trained and untrained muscle. Compared with transport in control rats subjected to an almost exhaustive single exercise session the day before experiment both maximum insulin- and contraction-stimulated transport rates were increased in all muscle types in trained rats. Accordingly, the increased glucose transport capacity in trained muscle was not due to a residual effect of the last ...
The Lawrence Livermore National Laboratory (LLNL) is establishing a local chapter of the University of California Energy Institute (UCEI). In order to most effectively contribute to the Institute, LLNL sponsored a workshop on energy and environmental issues in transportation. This workshop took place in Livermore on August 10 and brought together researchers from throughout the UC systems in order to establish a joint LLNL-UC research program in transportation, with a focus on energy and environmental impacts.
An interpretive theory of trace impurity transport in tokamaks is presented. It is based on an earlier phenomenological approach developed by the authors for describing tokamak plasma transport experiments. The present model is used to explain disparate phenomena observed in ALCATOR C and DITE in a unified phenomenological framework. Predictive numerical calculations are presented for DITE conditions. If experimentally confirmed, the model could be used to correlate plasma properties with trace impurity behaviour. (orig.)
This report highlights four main points. (1) A residue substitution in phosphoribulokinase of Synechocystis PCC 6803 renders the mutant light-sensitive. The authors isolated a light-sensitive mutant (BRLS) of the photosynthetic cyanobacterium Synechocystis 6803 that does not survive exposure to bright light; 70% of BRLS cells die upon exposure to light of > 3000 lux for 2 hr. (2) Excitation energy transfer from phycocyanin to chlorophyll in an apcA-defective mutant of Synechocystis sp. PCC 6803. A greenish mutant of the normally bule-green cyanobacterium Synechocystis sp. PC 6803, designated UV6p, was isolated and characterized. UV6p possesses functional photosystems I and II but lacks normal light harvesting phycobilisomes because allophycocyanin is absent and core-specific linker proteins are almost entirely absent. (3) Deletion of the psbG1 gene of the cyanobacterium Synechocystis sp. PCC 6803 leads to the activation of the cryptic psbG2 gene. The genes psbG1 and psbG2 in ...
Jittery (ji) is a recessive mouse mutation on Chromosome 10 characterized by progressive ataxic gait, dystonic movements, spontaneus seizures, and death by dehydration/starvation before fertility. Recently, a viable neurological recessive mutation, hesitant, was discovered. It is characterized by hesitant, uncoordinated movements, exaggerated stepping of the hind limbs, and reduced fertility in males. In a complementation test and by genetic mapping we have shown here that hesitant and jittery are allelic. Using several large intersubspecific backcrosses and intercrosses we have genetically mapped ji near the marker Amh and microsatellite markers D10Mit7, D10Mit21, and D10Mit23. The linked region of mouse Chromosome 10 is homologous to human 19p13.3, to which several human ataxia loci have recently been mapped. By excluding genes that map to human 21q22.3 (Pfkl) and 12q23 (Nfyb), we conclude that jittery is not likely to be a genetic mouse model for human ...
Metagenomics projects based on shotgun sequencing of populations of micro-organisms yield insight into protein families. We used sequence similarity clustering to explore proteins with a comprehensive dataset consisting of sequences from available databases together with 6.12 million proteins predicted from an assembly of 7.7 million Global Ocean Sampling (GOS) sequences. The GOS dataset covers nearly all known prokaryotic protein families. A total of 3,995 medium- and large-sized clusters consisting of only GOS sequences are identified, out of which 1,700 have no detectable homology to known families. The GOS-only clusters contain a higher than expected proportion of sequences of viral origin, thus reflecting a poor sampling of viral diversity until now. Protein domain distributions in the GOS dataset and current protein databases show distinct biases. Several protein domains that were previously categorized as kingdom specific are shown to have GOS examples in ...
Purpose : To detect differentially expressed genes in the patients with uterine cervical cancer during the radiation therapy. Materials and Methods : In patients with biopsy proven uterine cervical cancer, we took a tumor tissue just before radiation therapy and at 40 minutes after external irradiation of 1.8 Gy. Total RNAs isolated from non-irradiated and irradiated tumor tissue samples were analyzed using the differential-display reverse transcription-polymerase chain reaction (DDRT-PCR). Complementary DNA (cDNA) fragments corresponding to differentially expressed messenger RNAs(mRNAs) were eluted, and cloned. The differential expression of the corresponding mRNAs was confirmed by reverse northern blot. Differentially expressed cDNA bands were sequenced. Nucleotide sequence data were analyzed in the Gene Bank and EMBL databases via the BLAST network server to identify homologies to known genes or cDNA fragments. Expression pattern of down-regulated clone was ...
A cDNA library was constructed using RNA isolated from the livers of chickens which had been treated with zinc. This library was screened with a RNA probe complementary to mouse metallothionein-I (MT), and eight chicken MT cDNA clones were obtained. All of the cDNA clones contained nucleotide sequences homologous to regions of the longest (375 bp) cDNA clone. The latter contained an open reading frame of 189 bp, and the deduced amino acid sequence indicates a protein of 63 amino acids of which 20 are cysteine residues. Amino acid composition and partial amino acid sequence analyses of purified chicken MT protein agreed with the amino acid composition and sequence deduced from the cloned cDNA. Amino acid sequence comparison establish that chicken MT shares extensive homology with mammalian MTs. Southern blot analysis of chicken DNA indicates that the chicken MT gene is not a part of a large family of related sequences, but rather is likely to be ...
Screening of a human placenta lambdagt11 library has led to the isolation of the cDNA for the human ..beta../sub 1/-adrenergic receptor (..beta../sub 1/AR). Used as the probe was the human genomic clone termed G-21. This clone, which contains an intronless gene for a putative receptor, was previously isolated by virtue of its cross hybridization with the human ..beta../sub 2/-adrenergic receptor (..beta../sub 2/AR). The 2.4-kilobase cDNA for the human ..beta../sub 1/AR encodes a protein of 477 amino acid residues that is 69% homologous with the avian ..beta..AR but only 54% homologous with the human ..beta../sub 2/AR. This suggests that the avian gene encoding ..beta..AR and the human gene encoding ..beta../sub 1/AR evolved from a common ancestral gene. RNA blot analysis indicates a message of 2.5 kilobases in rat tissues, with a pattern of tissue distribution consistent with ..beta../sub 1/AR binding. This pattern is quite distinct from the ...
The DNA sequence motif ATTTGCAT (octamer) or its inverse complement has been identified as an evolutionarily conserved element in the promoter region of immunoglobulin genes. Two major DNA-binding proteins that bind in a sequence-specific manner to the octamer DNA sequence have been identified in mammalian species--a ubiquitously expressed protein (Oct-1) and a lymphoid-specific protein (Oct-2). During characterization of the promoter region of the chicken immunoglobulin light chain gene, the authors identified two homologous octamer-binding proteins in chicken B cells. when the cloning of the human gene for Oct-2 revealed it to be a member of a distinct family of homeobox genes, they sought to determine if the human Oct-2 cDNA could be used to identify homologous chicken homeobox genes. Using a human Oct-2 homeobox-specific DNA probe, they were able to identify 6-10 homeobox-containing genes in the chicken genome, demonstrating that the ...
Exogenous adenosine 5{prime}-triphosphate (ATP) mobilized intracellular calcium in human carcinoma A43l cells and in Swiss 3T3 and 3T6 mouse fibroblasts by increasing inositol trisphosphate similar to well down growth factors (platelet-derived growth factor (PDGF), epidermal growth factor (EGF), bradykinin (BK), serum). Calcium mobilization was examined by video imaging of fura-2 fluorescence is single cells, following the radioactive isotope {sup 45}Ca, and monitoring the decrease in fluorescence of cells loaded with chlortetracycline. Uridine 5{prime}-triphosphate, but not other nucleotides, mimicked ATP. Single-cell analysis revealed synchronous responses in 10 sec to ATP, BK or serum, while PDGF (3T3) and EGF (A431) produced slower signals with significant cell-to-cell variation. PDGF desensitized 3T3 cells to ATP and BK added 100 sec later but ATP or BK did not desensitized to PDGF. Homologous desensitization was seen with all agonists. Heterologous ...
Seed dormancy is an adaptive mechanism and an important agronomic trait. Temperature during seed development strongly affects seed dormancy in wheat (Triticum aestivum) with lower temperatures producing higher levels of seed dormancy. To identify genes important for seed dormancy, we used a wheat microarray to analyze gene expression in embryos from mature seeds grown at lower and higher temperatures. We found that a wheat homolog of MOTHER OF FT AND TFL1 (MFT) was upregulated after physiological maturity in dormant seeds grown at the lower temperature. In situ hybridization analysis indicated that MFT was exclusively expressed in the scutellum and coleorhiza. Mapping analysis showed that MFT on chromosome 3A (MFT-3A) colocalized with the seed dormancy quantitative trait locus (QTL) QPhs.ocs-3A.1. MFT-3A expression levels in a dormant cultivar used for the detection of the QTL were higher after physiological maturity; this increased expression correlated with a ...
In order to evaluate postclosure off-site doses from low-level radioactive waste disposal facilities, a computer code was developed to simulate the radionuclide released from waste form, transport through vadose zone and transport in the saturated zone. This paper describes the methodology used to model these process. The radionuclide released from the waste is calculated using a model based on first order kinetics and the transport through porous media was determined using semi-analytical solution of the mass transport equation, considering the limiting case of unidirectional convective transport with three-dimensional dispersion in an isotropic medium. The results obtained in this work were compared with other codes, showing good agreement. (author)
The objective of this research is to replace the current flow regime dependent interfacial area correlations in the thermal-hydraulic system analysis codes with an interfacial area transport equation, which dynamically models two-phase flow regime transitions. The interfacial area transport equation applicable to bubbly flows has been developed based on mechanistic modeling of bubble interaction. Detailed experiments have been performed for bubbly conditions in 2.54, 5.08 and 10.16-cm ID pipes to benchmark the model. This new approach predicts the continuous changes of the interfacial area and will eliminate artificial bifurcations stemming from the use of the static flow regime transition criteria. This paper presents the preliminary results of incorporating the one-group transport equation into the USNRC consolidated code, currently under development. The framework for the two-group transport equation ...
This volume contains papers delivered at a technical conference of the American Society of Civil Engineers held on June 25-27, 1991 in Seattle, Washington. The conference dealt with the physical aspects of sediment processes in the coastal environment. The papers are presented under three general themes: fundamentals of coastal sediment transport, coastal geologic processes, and engineering applications. The topics of the role of long waves in coastal sediment processes and cross-shore transport are highlighted. Examples of the topics covered include: sediment transport; beach and nearshore sedimentation; shoreline and beach profile changes; longshore and cross-shore transport; storm erosion; inlet sediment processes; gravel transport; coastal structure and sediment interaction; and dredging.
This paper examines the feasibility of returning coal mine wastes to the mined-out areas, as encouraged by PL 95-87, the ''Surface Mining Control and Reclamation Act of 1977'' (SMCRA). Reviews a National Academy of Sciences report which identified alternative backfilling methods. Presents diagram of mechanical backfilling with gravity and mechanical transport, and tables of existing underground mine waste disposal methods and a summary of technical feasibility of alternative disposal systems. Evaluated systems include gravity disposal with mechanical surface transport; gravity disposal with mechanical in-mine transport; complete mechanical disposal; mechanical disposal with gravity surface to underground transport; direct hydraulic disposal; hydraulic disposal with natural head; hydraulic disposal with artificial head; pneumatic disposal with mechanical surface ...
The transport-relevant basic data recorded within the framework of a federation-wide transport data survey made in 1986 are the best-founded source at present for determining the transport volume of other radioactive materials in different economic areas of the Federal Republic of Germany. Due to the high recording rates, a sufficiently complete survey can be provided in particular with regard to the transport volume (e.g.transports/consignments/cargos, transport distance) - with the exception of transport by ship - for the ranges of application in reserach, medicine and technology. However, as regards the radiologically relevant basic data and transport modalities, the information supplied by the transporting agencies is incomplete. A table gives a comprehensive survey of type and scope of the ...
Greenhouse gas emissions from international maritime transport are exempt from liabilities under the Kyoto Protocol. Research into quantifying these emissions is ongoing, and influences policy proposals to reduce emissions. This paper presents a cargo-based analysis of fuel consumption and greenhouse gas emissions from New Zealand's international maritime transport of goods. Maritime transport moves 99.5% (by mass) of New Zealand's internationally traded products. It is estimated that 73% of visiting vessels' activity can be directly attributed to the movement of goods in and out of New Zealand. A cargo-based methodology was used to estimate that the international maritime transport of New Zealand's imports and exports consumed 2.5 million tonnes (Mt; 2.6 billion litres) of fuel during the year 2007, which generated 7.7 Mt of carbon dioxide (CO_2) emissions. Double-counting of emissions would occur if a ...
The present conference on U.S. space transportation systems development discusses opportunities for aerospace students in prospective military, civil, industrial, and scientific programs, current strategic conceptualization and program planning for future U.S. space transportation, the DOD space transportation plan, NASA space transportation plans, medium launch vehicle and commercial space launch services, the capabilities and availability of foreign launch vehicles, and the role of commercial space launch systems. Also discussed are available upper stage systems, future space transportation needs for space science and applications, the trajectory analysis of a low lift/drag-aeroassisted orbit transfer vehicle, possible replacements for the Space Shuttle, LEO to GEO with combined electric/beamed-microwave power from earth, the National Aerospace Plane, laser propulsion to earth ...
The microbial transport simulator (MTS) is a three-dimensional, three-phase, multiple-component numerical model that permits the study of the transport of microorganisms and nutrients in porous media. Microbial parameters incorporated into MTS include: Microbial growth and decay, microbial deposition, chemotaxis, diffusion, convective dispersion, tumbling, and nutrient consumption. Governing equations for microbial and nutrient transport are coupled with continuity and flow equations under conditions appropriate for a black oil reservoir. The model`s mathematical formulations and preparation procedures of data files for conducting simulations using MTS are described. A general background of microbial transport simulation is given in Section I and the governing equations, mechanisms, and numerical solutions of MTS are given in Section II. Explanations for preparing an input data file with reservoir and ...
In the Fourth Dutch National Environmental Outlook (NMP, abbreviated in Dutch) of the National Institute of Public Health and the Environment (VROM), published in July 1997, possible future developments in the traffic and transport sector in the Netherlands are described for the period 1995-2020 and also evaluates present Dutch policies. NMP-4 also offers possible solutions for expected environmental problems. This report serves as a background document for the traffic and transport sector. With a view to present Dutch policies, the main conclusions drawn from the Outlook are that: (1) the policy targets for car and lorry use for 2010 will not be met, (2) the target for CO2 emissions from road transport for 2010 will not be met, (3) the NOx emission target for 2010 will be met for cars, but not for trucks, (4) the VOC emission target for 2010 will be met for cars, but not for trucks, and (5) the noise targets for road ...
More than 30 environmental concerns were analyzed for the transportation of coal by rail, roads (trucks), high voltage transmission lines (that is, from mine-mouth generating plants to distribution networks), coal slurry pipelines, and barges. The following criteria were used to identify these problems: (1) real physical environmetal impacts for which control technologies must be developed, or regulation made effective where control technologies presently exist; (2) the level of impact is uncertain, although the potential impact may be moderate to high; (3) the concerns identified by the first two criteria are specific to or exacerbated by coal transportation. Generic transportation problems are not included. The significant environmental problems identified as a result of this study are: (1) rail transport - community traffic disruption and human health, safety, and habitat destruction; (2) coal haul ...
The Transportation Legislative Database (TLDB) is an on-line information service containing detailed information on legislation and regulations regarding the transportation of radioactive materials in the United States. The system is dedicated to serving the legislative and regulatory information needs of the US Department of Energy and other federal agencies; state, tribal, and local governments; the hazardous materials transportation industry; and interested members of the general public. In addition to the on-line information service, quarterly and annual Legal Developments Reports are produced using information from the TLDB. These reports summarize important changes in federal and state legislation, regulations, administrative agency rulings, and judicial decisions over the reporting period. Information on significant legal developments at the tribal and local levels is also included on an as-available basis. ...
The cellular transport systems and the transport kinetics of ["1"2"3I]IMT uptake into non-malignant extracranial cells were characterized for the first time. Human fibroblasts were chosen as non-malignant extracranial cells as they are found ubiquitous in the body. ["1"2"3I]IMT is exclusively transported into fibroblasts via the sodium independent system L. An apparent Michaelis constant K_m=116.2#+-#18.9 #mu#M and a maximum transport velocity V_m_a_x=191.6#+-#13.9 pmol * (10"6 cells)"-"1 * min"-"1 were calculated for the sodium-independent transport. These results were compared with those determined in two malignantly transformed extracranial cell lines, the human Ewing's sarcoma cell lines VH-64 and CADO-ES-1.
Volume VII of the documentation for the Phase I Data Analysis Task performed in support of the current Regional Flow Model, Transport Model, and Risk Assessment for the Nevada Test Site Underground Test Area Subproject contains the tritium transport model documentation. Because of the size and complexity of the model area, a considerable quantity of data was collected and analyzed in support of the modeling efforts. The data analysis task was consequently broken into eight subtasks, and descriptions of each subtask's activities are contained in one of the eight volumes that comprise the Phase I Data Analysis Documentation.
The paper describes transportation cost of the nuclear off-peak power for a hydrogen production based on water electrolysis in Japan. The power could be obtainable by substituting hydropower and/or fossil fueled power supplying peak and middle demands with nuclear power. The transportation cost of the off-peak power was evaluated to be 1.42 yen/kWh when an electrolyser receives the off-peak power from a 6kV distribution wire. Marked reduction of the cost was caused by the increase of the capacity factor. (author)
The properties of a helium film on the surface of solid parahydrogen are investigated. It is shown that wetting of the solid hydrogen by the liquid helium occurs. The transport velocities along the He II film on the solid parahydrogen surface are measured in broad temperature, film height, and level difference ranges. It is shown that the transport velocity in this case has the least value as compared with its value on other substrates. The thickness of the helium-saturated film is determined on the solid hydrogen surface on the basis of the data obtained, and the value is in good agreement with the results of a computation performed within the framework of the Frenkel' theory.
A compact and energy independent solar-powered refrigeration unit was to be developed to store and transport small quantities of high-quality commodities needing refrigeration. This unit comprises the following matched components: thermo-electrical refrigerator unit, solar generator and transport equipment. The solar generator is a specially developed panel with 36 cells. This unit may best be used in human medicine, veterinary medicine, environmental protection analysis, laboratories and leisure time activities. (BWI)
Additional analyses and investigations were conducted to further define transportation system concepts that will be needed for the developmental and operational phases of an SPS program. To accomplish these objectives, transportation systems such as the Shuttle and its derivatives were identified new heavy lift launch vehicle (HLLV) concepts, cargo and personnel orbital transfer vehicles (EOTV and POTV), and intraorbit transfer vehicle (IOTV) concepts were evaluated and, to a limited degree, the program implications of their operations and costs were assessed. The results of these analyses were integrated into other elements of the overall SPS concept definition studies.
Theoretical analysis of the electric field bifurcation is made for the LHD plasma. For given shapes of plasma profiles, a region of bifurcation is obtained in a space of the plasma parameters. In this region of plasma parameters, the electric field domain interface is predicted to appear in the plasma column. The reduction of turbulent transport is expected to occur in the vicinity of the interface, inducing a internal transport barrier. Within this simple model, the plasma with internal barriers is predicted to be realized for the parameters of T{sub e}(0) {approx} 2 keV and n(0) {approx_equal} 10{sup 18} m{sup -3}. (author)
The Systems Design Study (SDS) identified technologies available for the remediation of low-level and transuranic waste stored at the Radioactive Waste Management Complex's Subsurface Disposal Area at the Idaho National Engineering Laboratory. The SDS study intentionally omitted the costs of transportation and disposal of the processed waste and the cost of decommissioning the processing facility. This report provides a follow-on analysis of the SDS to explore the basis for life-cycle cost segments of transportation, disposal, and facility decommissioning; to determine the sensitivity of the cost segments; and to quantify the life-cycle costs of the 10 ex situ concepts of the Systems Design Study.
The Systems Design Study (SDS) identified technologies available for the remediation of low-level and transuranic waste stored at the Radioactive Waste Management Complex`s Subsurface Disposal Area at the Idaho National Engineering Laboratory. The SDS study intentionally omitted the costs of transportation and disposal of the processed waste and the cost of decommissioning the processing facility. This report provides a follow-on analysis of the SDS to explore the basis for life-cycle cost segments of transportation, disposal, and facility decommissioning; to determine the sensitivity of the cost segments; and to quantify the life-cycle costs of the 10 ex situ concepts of the Systems Design Study.
With major D&D projects ongoing or being planned, and also with the daily management of radwaste from nuclear facilities, the potential role of transport packagings has often been overlooked: here will one rely essentially on drums, there several local waste processing units are built, elsewhere decommissioned facilities are cut in small bits to fit into small containers by far less efficient. The present paper proposes to illustrate how integrating a transport system from the start may influence operational choices of waste management.
Computer software for risks assessment of transportation of important freight has been developed. It incorporates models of transport accidents, including terrorist attacks. These models use, among the others, unput data of cartographic character. Geographical information system technology and electronic maps of an area are involved as an instrument for handling this kind of data. Fuzzy set theory methods as well as standard methods of probability theory have been used for quantitative risk assessment. Fuzzy algebraic operations and their computer realisation are discussed. One preliminary example of risk assessment is described. (authors)
The validity is given to the newly proposed two {delta}f method for neoclassical transport calculation, which can be solve the drift kinetic equation considering effects of steep plasma gradients, large radial electric field, finite banana width, and an orbit topology near the axis. The new method is applied to the study of ion transport with steep plasma gradients. It is found that the ion thermal diffusivity decreases as the scale length of density gradient decreases, while the ion particle flux due to ion-ion self collisions increases with increasing gradient. (author)
The dual-media approach to contaminant transport modeling has emerged over the years as a clear improvement over the conventional Fickian advection-dispersion, or single-medium model, in an increasing number of settings. Recent application to the MADE-2 tracer test at Columbus Air Force Base has extended interest to field-scale plume migration in unconsolidated sediments. Subsequent application to tritium migration from the SRS F- and H-area seepage basins within this study also demonstrated improved transport predictions.
A versatile method for estimating the characteristics of radon transport in soil is developed. The method allows the following characteristics to be estimated: depth distribution function of the soil gas radon concentration, equilibrium radon concentration in the soil air, depth at which the radon concentration reaches its equilibrium value, radon flux density from the Earth's surface, and convective radon transport velocity. The method is based on soil gas radon concentration measurements and is appropriate in the case of relatively uniform geology. (orig.)
The presence of endothelin (ET)-like immunoreactivity and the cardiovascular effects of mammalian ET-1 in fish have been reported. To identify ET-related peptides in fish, we screened the cDNA library of the salmon (Oncorhynchus keta) stomach by means of rapid amplification of cDNA ends, and we cloned cDNAs encoding an ET-related peptide. The salmon ET-related sequence of 21 amino acids is identical to the trout ET-1 peptide recently purified from kidney specimens of Oncorhynchus mykiss. The deduced amino acid sequence of salmon pre-proET-1 (PPET-1) comprises 244 amino acids, including a putative signal sequence and mature ET-1, as well as big ET-1 and ET-1-like sequences. This precursor, the first reported PPET-1 sequence for Salmoniformes, Teleostei, has low homology with the sequences of human, mouse, frog (Xenopus laevis), and zebrafish (Danio rerio) PPET-1 (26%, 29%, 24%, and 39%, respectively). PMID:16740985
Directed evolution of proteins depends on the production of molecular diversity by random mutagenesis. While a number of methods have been developed for introducing this diversity, the best ways to sample it are not always clear. Here we used simple statistics to analyse completeness and diversity in randomized libraries generated by oligonucleotide-directed mutagenesis, error-prone polymerase chain reaction (epPCR) and in vitro recombination of highly homologous sequences. For oligonucleotide-directed mutagenesis, we derive equations to estimate how complete a given library is expected to be and also to predict the size of library required to give a fixed probability of being 100% complete. We describe the statistical bases for computer programs which estimate the number of distinct variants represented in epPCR and shuffled libraries, dubbed PEDEL and DRIVeR, respectively. These programs allow the user to calculate (rather than guess) the diversity represented in ...
Escherichia coli spheroplast protein y (EcSpy) is a small periplasmic protein that is homologous with CpxP, an inhibitor of the extracytoplasmic stress response. Stress conditions such as spheroplast formation induce the expression of Spy via the Cpx or the Bae two-component systems in E. coli, though the function of Spy is unknown. Here, we report the crystal structure of EcSpy, which reveals a long kinked hairpin-like structure of four ?-helices that form an antiparallel dimer. The dimer contains a curved oval shape with a highly positively charged concave surface that may function as a ligand binding site. Sequence analysis reveals that Spy is highly conserved over the Enterobacteriaceae family. Notably, three conserved regions that contain identical residues and two LTxxQ motifs are placed at the horizontal end of the dimer structure, stabilizing the overall fold. CpxP also contains the conserved sequence motifs and has a predicted secondary structure similar ...
Abstract SLC9A9 (solute carrier family 9, member 9, also known as Na+/H+ exchanger member (NHE9)) is a membrane protein that regulates the luminal pH of the recycling endosome, an essential organelle for synaptic transmission and plasticity. SLC9A9 has been implicated in human attention deficit hyperactivity disorder (ADHD) and in rat studies of hyperactivity. We examined the SLC9A9 gene sequence and expression profile in prefrontal cortex, dorsal striatum and hippocampus in two genetic rat models of ADHD. We report two mutations in a rat model of inattentive ADHD, the WKY/NCrl rat, which affect the interaction of SLC9A9 with calcineurin homologous protein (CHP). We observed an age-dependent abnormal expression of SLC9A9 in brains of this inattentive model and in the Spontaneous Hypertensi...
The fluorescent behaviour and the photodynamic effect was studied in native and structurally modified lysozyme and ..cap alpha..-lactalbumin. The Tyr residues in lysozyme and ..cap alpha..-lactalbumin show different sensitivities to the photodynamic effect. The effect is zero in the case of Tyr from native lysozyme. In contrast, the Tyr residues in ..cap alpha..-lactalbumin are susceptible to photooxidation, which indicates a greater degree of exposure to the solvent. The three His residues of ..cap alpha..-lactalbumin have different degrees of exposure and show two different kinetics of photooxidation whereas the His residue of lysozyme is photooxidized with a single kinetic. Two photooxidation kinetics were obtained for the Trp residues of both native proteins, an indication that in both cases there are Trp residues that are differently exposed to the solvent. The wavelengths of maximum fluorescent emission of the Trp residues were different for the two proteins, an effect which can ...
Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. Recent cloning of the murine and porcine radixin cDNAs revealed a protein highly homologous to ezrin and moesin. The authors have cloned and sequenced the human radixin cDNA and found the predicted amino acid sequence for the human protein to be nearly identical to those predicted for radixin in the two other species. By Southern analyses of Chinese hamster x human somatic cell hybrid DNA and of PCR products derived from hybrids, the coding gene (RDX) was mapped to 11q. Fluorescence chromosomal in situ hybridization with a cDNA plasmid further localized this gene to band 11q23. However, PCR amplification with [open quotes]radixin-specific[close quotes] primers on the hybrid DNA panel yielded an additional, very similar DNA sequence that was further characterized by direct sequencing of PCR products. This sequence represents a truncated version and the respective locus ...
Goosecoid is a homeobox gene first isolated from a Xenopus dorsal lip cDNA library. Homologous genes have been isolated from mouse, zebrafish, and chick. In all species examined, the gene is expressed and plays an important role during the process of gastrulation in early embryonic development. The authors report here the cloning of the human goosecoid (GSC) from a genomic library and the sequence of its encoded protein. The genomic organization and protein sequence of the human gene are highly conserved with respect to those of its Xenopus and mouse counterparts: all three genes consist of three exons, with conserved exon-intron boundaries. The sequence of the homeo-domain is 100% conserved in most vertebrates. Using somatic cell hybrid and chromosomal in situ hybridization, the gene was mapped to chromosome 14q32.1. 30 refs., 3 figs., 2 tabs.
In this article we describe the identification of endophytic bacteria belonging to three groups isolated from shoot tip cultures of banana cv. Grand Naine in a recent study (Thomas et?al. 2008) based on partial 16S rRNA gene sequence homology analysis. The first group included banana stocks that displayed obvious colony growth on MS based tissue culture medium during the first in?vitro passage. The second group constituted stocks that were tissue index-negative for cultivable bacteria initially but turned index-positive after a few to several (4?8) in?vitro passages while the third group formed one sub-stock that turned index-positive after about 18 passages. The organisms belonged to about 20 different genera comprising of ?, ?, ?-proteobacteria, Gram-positive firmicutes and actinobacteri...
1. Gamma globulin metabolism and distribution were studied employing rabbit gamma globulin (RGG)I(131) 24 times in 13 control rabbits. Similar studies were performed before and during the ananmestic response in 4 rabbits previously sensitized with a polyvalent pneumococcal vaccine. 2. During the ananmestic response, gamma-globulin levels increased from 1.0 to 6.0 gm/100 ml, and the gamma-globulin pool increased from 0.7 to 4.7 gm/kg. There was no change in the intravascular-extravascular partition of gamma globulin. 3. Gamma globulin degradation increased from 0.06 to 0.33 gm/kg/day during the 28 days of the immunization period while gamma globulin synthesis increased even further to average 0.47 gm/kg/day. Following the attainment of elevated gamma globulin levels the fractional rate of RGG-I(131) turnover increased from 8.0 to 12.5 per cent/day. 4. No differences were noted in the metabolism of homologous or autologous gamma globulin regardless of the allotypic ...
This report describes the fiscal 1998 result on development of genome informatics technology. As comparative analysis technique of genes, the combination of electrophoresis and PCR was used. For improvement of the throughput and reproducibility of the technique, module- shuffling primers were used, and the multi(96)-arrayed capillary fragment analyzer was devised. The system detecting SNPs rapidly was also developed successfully. As analysis technology of DNA sequence by use of triple- stranded DNA formation, study was made on construction of long cDNA libraries, selective subtraction of specific sequences from libraries, and the basic technology of homologous cloning. Study was also made on each reaction step of IGCR technique for fast analysis, and specifications of a fluorescence transfer monitor. As modeling technique of genetic sequence information, the simulation model was developed for gene expression regulatory networks during muscle differentiation, and ...
A tk{sup +/-} mouse embryonic stem (ES) cell line, designated 1G2, has been created in which one allele of the thymidine kinase (tk) gene was inactivated by targeted homologous recombination. This line is an analog of the mouse lymphoma tk{sup +/-} L5178Y cell line, which is used widely to assess the mutagenicity of chemical agents. Treatment of 1G2 cells with the alkylating agent N-ethyl-N-nitrosourea (ENU) resulted in a dose-related increase in tribluorothymidine-resistant colonies. Mutant frequencies of 152 and 296 per 10{sup 6} cells were determined for 0.1 and 0.3 mg/ml doses of ENU, compared with a spontaneous mutant frequency of 15 per 10{sup 6} cells. The data indicate that tk{sup +/-} 1G2 ES cells may be useful for the creation of a transgenic mouse model for assessing in vivo mutation using an endogenous autosomal gene. 45 refs., 2 figs., 1 tab.
The classical phenomenon of position-effect variegation (PEV) is the mosaic expression that occurs when a chromosomal rearrangements moves a euchromatic gene near heterochromatin. A striking feature of this phenomenon is that genes far away from the junction with heterochromatin can be affected, as if the heterochromatic state {open_quotes}spreads.{close_quotes} We have investigated classical PEV of a Drosophila brown transgene affected by a heterochromatic junction {approximately} 60 kb away. PEV was enhanced when the transgene was locally duplicated using P transposase. Successive rounds of P transpose mutagenesis and phenotypic selection produced a series of PEV alleles with differences in phenotype that depended on transgene copy number and orientation. As for other examples of classical PEV, nearby heterochromatin was required for gene silencing. Modifications of classical PEV by alterations at a single site are unexpected, and these observations contradict models for spreading ...
We report the isolation of cDNA clones for the mouse {alpha}7 neuronal nicotinic acetylcholine receptor subunit (gene symbol Acra7), the only nicotinic receptor subunit known to bind a-bungarotoxin in mammalian brain. This gene may have relevance to nicotine sensitivity and to some electrophysiologic findings in schizophrenia. The mouse {alpha}7 subunit gene encodes a protein of 502 amino acids with substantial identity to the rat (99.6%), human (92.8%), and chicken (87.5%) amino acid sequences. The {alpha}7 gene was mapped to mouse chromosome 7 near the p locus with the following gene order from proximal to distal: Myod1-3.5 {+-}1.7 cM-Gas2-0.9 cM {+-} 0.9 cM-D7Mit70-1.8 {+-} 1.2 cM- Acra7-4.4 {+-}1.0 cM-Hras1-ps11/Igf1r/Snrp2a. The human gene was confirmed to map to the homologous region of human chromosome 15q13-q14. 26 refs., 3 figs.
Regulation of transcription initiation by RNA polymerase II requires TFIID, a multisubunit complex composed of the TATA binding protein (TBP) and at least seven tightly associated factors (TAFs). Some TAFs act as direct targets or coactivators for promoter-specific activators while others serve as interfaces for TAF-TAF interactions. Here, we report the molecular cloning, expression and characterization of Drosophila dTAFII60 and its human homolog, hTAFII70. Recombinant TAFII60/70 binds weakly to TBP and tightly to the largest subunit of TFIID, TAFII250. In the presence of TAFII60/70, TBP and TAFII250, a stable ternary complex is formed. Both the human and Drosophila proteins directly interact with another TFIID subunit, dTAFII40. Our findings reveal that Drosophila TAFII60 and human TAFII70 share a high degree of structural similarity and that their interactions with other subunits of TFIID are conserved.Images
The heterokaryotic and vegetative diploid phases of Magnaporthe grisea, a fungal pathogen of grasses, have been characterized. Hyphal tip cells and conidia (vegetative spores) taken from these heterokaryons are auxotrophs with phenotypes identical to one or the other of the parents. M. grisea heterokaryons have completely septate hyphae with a single nucleus per cell. Heterokaryons have been utilized for complementation and dominance testing of mutations that affect nutritional characteristics of the fungus. Heterokaryons growing on minimal medium spontaneously give rise to fast-growing sectors that have the genetic properties expected of unstable heterozygous diploids. In fast-growing sectors, most hyphal tip cells are unstable prototrophs. The conidia collected from fast-growing sectors include stable and unstable prototrophs, as well as auxotrophs that exhibit a wide range of phenotypes, including many recombinant classes. Genetic linkage in meiosis has been detected between two ...
Aims: In this study we investigated whether attenuation of endoplasmic reticulum stress (ER stress) could protect HepG2 cells from free fatty acid (FFA)-induced apoptosis. Main methods: Human liver cell line HepG2 cells were exposed to Sodium Palmitate (Pa) or Sodium Oleate (Ol). Apoptosis and ER stress of HepG2 cells were analyzed with flow cytometry, real-time RT-PCR and Western Blotting. An expression plasmid encoding for the ER chaperone immunoglobulin heavy chain-binding protein (Bip) was transfected into HepG2 cells to attenuate ER stress. Small interfering RNA siCHOP was used to knockdown the expression of C/EBP Homologous Protein (CHOP) in HepG2. Key findings: Pa led to cytotoxicity and apoptosis in HepG2 cells in a dose-dependent pattern and also induced ER stress indicated by inc...
Attention deficit disorder (ADHD) is a complex biobehavioral phenotype which affects up to 8% of the general population and often impairs social, academic, and job performance. Its origins are heterogeneous, but a significant genetic component is suggested by family and twin studies. The murine strain, coloboma, displays a spontaneously hyperactive phenotype that is responsive to dextroamphetamine and has been proposed as a genetic model for ADHD. Coloboma is a semi-dominant mutation that is caused by a hemizygous deletion of the SNAP-25 and other genes on mouse chromosome 2q. To test the possibility that the human homolog of the mouse coloboma gene(s) could be responsible for ADHD, we have carried out linkage studies with polymorphic markers in the region syntenic to coloboma (20p11-p12). Five families in which the pattern of inheritance of ADHD appears to be autosomal dominant were studied. Segregation analysis of the traits studied suggested that the best ...
Without using any moduli, sheaves, stacks, nor any analytic, nor category-type arguments, we exhibit an analogue to Geometric Langlands Theory in an entirely model-independent, non-perturbative,purely smooth topological context in Artin Presentation Theory. A basic initial feature is that AP Theory, as a whole, is already, ab initio, a universal canonical 2D sigma-model, targeting smooth, compact, simply-connected 4-manifolds with a connected boundary, and its topological Planckian quantum starting point, as well as its cone-like, infinitely-generated at each stage, graded group of homology-preserving, but topology-changing transitions/interactions, exhibit the most general qualitative S-duality. We first point out the numerous mathematically rigorous, model-free, (i.e., intrinsic), topological AP analogues with the heuristic Kapustin-Witten version of Geometric Langlands theory, as well as the crucial differences between the two theories. The latter have to exist ...
Ion neoclassical transport with finite orbit width dynamics is calculated over whole poloidal cross section by using accurate {delta}f method which employs an improved like-particle collision operator and an accurate weighting scheme to solve drift kinetic equation. Ion thermal transport near magnetic axis shows a great reduction from its conventional neoclassical level due to non-standard orbit topology, like that of previous {delta}f simulation. On other hand, the direct particle loss from confinement region may strongly increase ion energy transport near the edge. It is found that ion parallel flow near the axis is also largely reduced due to non-standard orbit topology. In the presence of steep density gradient, ion thermal conductivity is significantly reduced, and an ion particle flux is driven by self-collision alone. (author)
Indoor air exposures to gaseous contaminants originating in soil can cause large human health risks. To predict and control these exposures, the mechanisms that affect vapor transport in near-surface soils need to be understood. In particular, radon exposure is a concern since average indoor radon concentrations lead to much higher risks than are generally accepted for exposure to other environmental contaminants. This dissertation examines an important component of the indoor radon problem: the impacts of wind on soil-gas and radon transport and entry into buildings. The research includes experimental and modeling studies of wind`s interactions with a building`s superstructure and the resulting soil-gas and radon flows in the surrounding soil. In addition to exploring the effects of steady winds, a novel modeling technique is developed to examine the impacts of fluctuating winds on soil-gas and radon transport.
We have fabricated nanometer-spaced electrodes on electron-transparent silicon nitride membranes. A thin Cr/Au layer is evaporated on the backside of the membrane which serves as a gate electrode. Using these devices, we have performed three-terminal electron transport measurements on gold nano-particles at liquid helium temperature. Coulomb Blockade features have been observed and the capacitance to the gate has been extracted. After transport measurements, the Cr/Au back gate is removed and the devices are inspected with a transmission-electron microscope (TEM). TEM inspection reveals the presence of a few nano-particles in the nanogap, which is in agreement with the transport measurements. In addition, the nano-particle size as observed by TEM coincides with the one estimated from the gate capacitance value.
... Chem. Res., in press, 2006. 8. Smith, BL, Bruno, TJ, Advanced distillation curve measurement with a model predictive temperature controller. Int. ...
The utilization of thermal fluctuations or Johnson/Nyquist noise as a spectroscopic method to determine transport properties in conductors or semiconductors is developed. The autocorrelation function is obtained from power spectral density measurements thus enabling electronic transport property calculation through the Green-Kubo formalism. This experimental approach is distinct from traditional numerical methods such as molecular dynamics simulations, which have been used to extract the autocorrelation function and directly related physics only. This work reports multi-transport property measurements consisting of the electronic relaxation time, resistivity, mobility, diffusion coefficient, electronic contribution to thermal conductivity and Lorenz number from experimental data. Double va...
SummaryMammalian iron homeostasis is regulated by the interaction of the liver-produced peptide hepcidin and its receptor, the iron transporter ferroportin. Hepcidin binds to...Full Text Available
HLW disposal is one of the largest issue to utilize Nuclear power safely. In the past study, the concept, which buffer materials and Overpacked waste were transported into underground respectively, have shown. The concept of pre-assembled engineered barrier has advantage to simplify the logistics and emplacement procedure, however there are difficulties to support heavy weight of pre-assembled package by equipment under the condition of little clearance between tunnel and package. In this study, Combination of air bearing and two degree-of-freedom wheels were suggested for transportation, and air jack was suggested for unloading and emplacement system. Also, whole system for transportation and emplacement procedure was designed, and Scale model test was examined to evaluate the feasibility of these concept and functions. (author)
"Report of the Ad Hoc Subpanel on Reusable Launch Vehicle Technology. ... The author comments that the United State's space transportation system is ...
Abstract Many solute carrier transporters that interact with anticancer agents and contribute to their pharmacokinetics have been shown to be differentially upregulated in cancer cells as a result of adaptive response to altered nutritional requirements. This review focuses on pathophysiological function of membrane transporters responsible for the influx of physiological substances including oligopeptides, amino acids, and organic cations and anions, and summarizes the recent knowledge regarding mechanisms in their gene expressions. Broad substrate specificity of enhanced oligopeptide H+/peptide cotransporter 1 activity in cancer cells is useful for tumor tissue-specific delivery of chemotherapeutic agents and positron emission tomography diagnostic probes. Amino acid transporters such as...
The oviduct is an exquisitely designed organ that functions in picking-up ovulated oocytes, transporting gametes in opposite directions to the site of fertilization, providing a suitable environment...Full Text Available
For coupled quantum wires and dots, tunneling effects and coherent transport for quantum computing are being studied. In 2D systems, electron-hole bilayers for exciton...
... rivers of the world, the Zaire is not an efficient transportation link because of many rapids and falls to negotiate between Kinshasa and the port city of Matadi. ...
Phospholemman (PLM), a member of the FXYD family of regulators of ion transport, is a major sarcolemmal substrate for protein kinases A and C in cardiac and skeletal muscle. In the heart, PLM...Full Text Available
The majority of Brazilian big cities served by conventional railroad systems with routes starting in the center of the URBIS, destinate them the long distances transport of passengers and cargo. Actually, these systems are being transformed to become commuter trains which join a great capacity of transportation to a much lower costs of implantation than subway ones. The most important characteristic of this train, when talking about energy consumption, is the possibility of using different kinds of explosion engines from three cycles: Diesel, mixed (Diesel + Natural Gas), and Otto moved by natural gas, besides the traditional electric traction. The use of natural gas in Otto cycle engines shows itself competitive from the point of view of investments, operational costs and environmental preservation. (author). 10 refs., 1 fig
o If retaining CSRS/FERS, transferred employee will be credited with accumulated sick leave o Be reimbursed for travel, transportation, relocation costs ...
D-11. The Contractor shall pack and mark all hardware deliverable under this ... Handling, and Transportation, and/or MIL-STD-2073-1 and MIL-STD 2073-2, ...
The Bantam System Technology Project is one element of the Advanced Space Transportation Program -- a NASA initiative to reduce the cost of space launch and ...
Iron, zinc, copper and manganese are essential metals for cellular enzyme functions while cadmium, mercury and the metalloid arsenic lack any biological function. Both, essential metals, at high concentrations, and non-essential metals and metalloids are extremely reactive and toxic. Therefore, plants have acquired specialized mechanisms to sense, transport and maintain essential metals within physiological concentrations and to detoxify non-essential metals and metalloids. This review focuses on the recent identification of transporters that sequester cadmium and arsenic in vacuoles and the mechanisms mediating the partitioning of these metal(loid)s between roots and shoots. We further discuss recent models of phloem-mediated long-distance transport, seed accumulation of Cd and As and rec...
Nov 21, 2003... for the European Space Agency's Automated Transport Vehicle, a new, uncrewed station cargo vehicle targeted for launch late next year. ...
Congestive heart failure (CHF) is a leading cause of morbidity and mortality. CHF is marked by atrial and ventricular enlargement and reduced cardiac contractility, as well as an association...Full Text Available
... from a single location. The growing cities of Matadi and Boma and maritime transportation, and the potential ... known only from the Lower Congo River, between Matadi and Boma. (click...
of different transportation fuels (e.g., gasoline, diesel fuel, aviation gasoline, jet fuel, residual fuel oil). Subsequent calculations are performed to estimate the share...
activities, including the combustion of diesel fuel in heavy-duty vehicles and jet fuel in aircrafts. Current details on emissions from the transportation and other...
... non- emissive guidance and collision alert, automated transport in dynamic environments, and assembly and inspection. Foveal Vision, Autonomous Agents, ...
This article discusses three largely unrecognized aspects related to fluid movement in ocular tissues; namely, a) the dynamic changes in water permeability observed in corneal and conjunctival...Full Text Available
cellulosic biofuel volumes for consumption as transportation fuel, heating oil, or jet fuel in the U.S. in 2011. We also evaluated the production and import potential for...
and used to reduce or replace petroleum-based transportation fuel, heating oil or jet fuel). In addition, this rule includes a new regulatory provision establishing a...
The symposium reviewed the use of electronics in mining today. Subjects covered include control systems; remote sensing; telemetry; data transmission; microprocessors and transportation systems. 15 papers have been abstracted separately.
This paper has two key objectives. Firstly, using the findings from case study research undertaken in Colombo (Sri Lanka), Faisalabad (Pakistan) and Dar es Salaam (Tanzania), the paper seeks to demonstrate the importance of an appropriate regulatory framework and effective mechanisms of enforcement for sustainable urban transport systems in developing countries. Secondly, the paper highlights the critical importance of communication and co-ordination between stakeholders (defined here as transport users, providers and regulators) if regulation is to be effective. The views of poor and disadvantaged passenger groups-women, children, the elderly and disabled-are used in the paper to illustrate the importance of transport systems to their livelihoods, such as work, education, health and socia...
Many methods have been proposed for the determination of the corrosion current density from polarization curves; the two most commonly used ones are the three-point method and the curve-fitting method. The basis of these methods is a polarization equation that was derived for the complete absence of mass-transport effect or considers the cathodic partial reaction to be under complete mass-transport control. The mixed-control case has been much less investigated, even though this case may quite frequently reflect practical situations. A completely generalized polarization equation is proposed that can be used as a basis for curve-fitting data evaluation under any conditions. This equation was used to determine the error caused by the neglect of mass-transport effect in conventional data evaluation.
The interfacial area transport equation dynamically models the two-phase flow regime transitions and predicts continuous change of the interfacial area concentration along the flow field. Hence, when employed in the numerical thermal-hydraulic system analysis codes, it eliminates artificial bifurcations stemming from the use of the static flow regime transition criteria. Accounting for the substantial differences in the transport phenomena of various sizes of bubbles, the two-group interfacial area transport equations have been developed. The group 1 equation describes the transport of small-dispersed bubbles that are either distorted or spherical in shapes, and the group 2 equation describes the transport of large cap, slug or churn-turbulent bubbles. The source and sink terms in the right-hand-side of the transport equations have been established by ...
Diffusion in the extracellular space (ECS) of the brain is constrained by the volume fraction and the tortuosity and a modified diffusion equation represents the transport behavior of many molecules...Full Text Available
Understanding the processes responsible for coastal change is important for managing our coastal resources, both natural and economic. The current scientific understanding of coastal sediment transport and geology suggests that examining coastal processes at regional scales can lead to significant insight into how the coastal zone evolves. To better identify the significant processes affecting our coastlines and how those processes create coastal change we developed a Coupled Ocean-Atmosphere-Wave-Sediment Transport (COAWST) Modeling System, which is comprised of the Model Coupling Toolkit to exchange data fields between the ocean model ROMS, the atmosphere model WRF, the wave model SWAN, and the sediment capabilities of the Community Sediment Transport Model. This formulation builds upon ...
... that avenue, there would be a disruption in supply, the general ... Transportation Command is responsible not only for supplying warfighters in direct ...
Figure 23 presented detailed layout of the cooling system designed for the Mach 6 hypersonic- transport. The distribution system consisted of thin-wall ...
Computer software for risk assessment of transportation of important freight has been developed. It incorporates models of transport accidents, including terrorist attacks. These models use, among the others, input data of cartographic character. Geographic information system technology and electronic maps of a geographic area are involved as an instrument for handling this kind of data. Fuzzy set theory methods as well as standard methods of probability theory have been used for quantitative risk assessment. Fuzzy algebraic operations and their computer realization are discussed. Risk assessment for one particular route of railway transportation is given as an example. (author)
A study was conducted to determine the impacts of climate change on the transportation sector in a regional context. The purpose was to analyze potential climate change impacts on demand for freight transportation in Atlantic Canada through its association with other sectors of the regional economy. Freight transportation is designed to meet transportation demands of commodity producers who must deliver their goods to final users. Since it is a by-product of profit maximization and cost minimization by producers of all types of commodities in various sectors of the economy, this study modelled climate change impacts as productivity shocks in relevant sectors of the economy. A computerized simulation was then used to determine how the consequences of these shocks influence the demand for freight transportation. This was accomplished by collecting climate related data in Atlantic ...
Abstract Remediation of ground water containing anionic contaminants presents a great challenge. Because of its low cost, surfactant-modified zeolite (SMZ) has been studied for >10 years for potential uses as permeable barrier materials to remove anionic contaminants from water. In this study, zeolite aggregates with particle size of 3.4 to 4.8 mm were modified by hexadecyltrimethylammonium (HDTMA) bromide, a cationic surfactant, to a surfactant loading level of 80 mmol/kg and a concurrent counterion bromide loading level of 34 mmol/kg. While no retardation of chromate transport was observed for unmodified columns, a retardation factor of 60 was found for chromate transport through the SMZ columns. Slow but persistent desorption of HDTMA occurred throughout the chromate transport experimen...
Substances cross the blood-brain barrier (BBB) by a variety of mechanisms. These include transmembrane diffusion, saturable transporters, adsorptive endocytosis, and the extracellular pathways. Here,...Full Text Available
our exploration of space, in a manner with improved safety. ... a new Space Transportation System. ... Columbia launches as STS-107 on January 16, 2003. ...
... is known from the Lower Congo River basin (Matadi), from Pool Malebo (Stanley Pool) and from the ... Marine transport and oil disposal by boats and Matadi pose threats in the region. When the water ... ...
This is a short version of a scenario study concerning the possibilities to reach an Environmentally Sustainable Transport system in Sweden in a perspective of 30 years. The aim of the scenario study has been to describe one of several possible paths from today`s transport system to an environmentally adopted one. However, this does not imply that the task is to predict how such a transformation can be accomplished. The aim is rather to illustrate what such transformation require in the form of political decisions. The transformation of the transport system in to an environmentally adopted one, is primarily treated as a political problem, and a political perspective has accordingly been chosen for the study. In this English version of the scenario, the carbon dioxide problem is used to illuminate the many conflicts in goals and other problem that will attend an environmental adoption of the Swedish ...
The ASFIT (Anisotropic Source-Flux Iteration Technique) code was first developed in India about 1970 to solve the radiation transport equation represented in the form of coupled integral equations separating the spatial and energy-angular transmissions. The ASFIT code uses a nodal structure in the wavelength [lambda] domain in Compton units (CU) rather than in the energy domain. The ASFIT-VARI code is the latest version available from the Radiation Shielding Information Center. It incorporates variable dimensioning and has been adapted for use on MS-DOS personal computers using the Ryan-McFarland or Microsoft Version 5.0 FORTRAN compilers. While earlier versions used point cross sections (well suited for gamma-ray transport), the present version also allows multigroup cross sections for neutron and coupled neutron-gamma-ray transport.
A 3-D hydrodynamic dispersion model for tracer transport is developed and implemented into the TOUGH2 EOS3 (T2R3D) module. The model formulation incorporates a full dispersion tensor, based on a 3-D velocity field with a 3-D, irregular grid in a heterogeneous geological system. Two different weighting schemes are proposed for spatial average of 3-D velocity fields and concentration gradients to evaluate the mass flux by dispersion and diffusion of a tracer or a radionuclide. This new module of the TOUGH2 code is designed to simulate processes of tracer/radionuclide transport using an irregular, 3-D integral finite difference grid in non-isothermal, three-dimensional, multiphase, porous/fractured subsurface systems. The numerical method for this transport module is based on the integral finite difference scheme, as in the TOUGH2 code. The major assumptions of the tracer transport module are: (a) a tracer ...
...Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION QUALIFICATION AND CERTIFICATION OF LOCOMOTIVE ENGINEERS Dispute Resolution Procedures ยง 240.405 Processing qualification review petitions. (a)...
ABSTRACT: BACKGROUND: The capacity to maintain internal ion homeostasis amidst changing conditions is particularly important for teleost fishes whose reproductive cycle is dependent upon movement from freshwater to seawater. Although the physiology of seawater osmoregulation in mitochondria-rich cells of fish gill epithelium is well understood, less is known about the underlying causes of inter- and intraspecific variation in salinity tolerance. We used a genome-scan approach in Arctic charr (Salvelinus alpinus) to map quantitative trait loci (QTL) correlated with variation in four salinity tolerance performance traits and six body size traits. Comparative genomics approaches allowed us to infer whether allelic variation at candidate gene loci (e.g., ATP1alpha1b, NKCC1, CFTR, and cldn10e) could have underlain observed variation. RESULTS: Combined parental analyses yielded genome-wide significant QTL on linkage groups 8, 14 and 20 for salinity tolerance performance traits, and 1, 19, 20 ...
Full text of publication follows:In the current thermal-hydraulic system analysis codes using the two-fluid model, the empirical correlations that are based on the two-phase flow regimes and regime transition criteria are being employed as closure relations for the interfacial transfer terms. Due to its inherent shortcomings, however, such static correlations are inaccurate and present serious problems in the numerical analysis. In view of this, a new dynamic approach employing the interfacial area transport equation has been studied. The interfacial area transport equation dynamically models the two-phase flow regime transitions and predicts continuous change of the interfacial area concentration along the flow field. Hence, when employed in the thermal-hydraulic system analysis codes, it eliminates artificial bifurcations stemming from the use of the static flow regime transition criteria. Therefore, the interfacial area ...
This report describes the results of a large project conducted in a corporation between Statistics Denmark and the Danish National Environmental Research Institute. The main objective of the project has been to analyse the possibilities of prescribing the development in the Danish freight transport in a more appropriate and precise way than it is done by existing models. A secondary objective of the project was to develop a model based on the findings of the analysis. The intention was to be able to describe all areas of freight transport. The analysis has proven it impossible to improve the existing calculations in some areas of transport. Hence, the project has been narrowed down to focus exclusively on road freight transport. The developed model distinguishes itself from existing models by a much higher level of detail in the calculations. This enables the model to describe the structural relations ...
In order to reduce the potential for severe liquid pipeline accidents, the U.S. Materials Transportation Bureau (MTB) proposes to require a hydrostatic test on all onshore pipelines carrying highly volatile liquids which have not been previously tested to at least 1.25 times their maximum operating pressure for at least 24 hr. Comments should be received by the MTB by 2/15/79. Late filed comments will be considered as far as practicable.
We solve an R -linear problem for a multiple-connected circular domain in a class of doubly periodic functions in analytic form by a method of functional equations. This problem models transport properties of two-dimensional composite materials made from a collection of disks embedded in an otherwise uniform host.
We solve an R -linear problem for a multiple-connected circular domain in a class of doubly periodic functions in analytic form by a method of functional equations. This problem models transport properties of two-dimensional composite materials made from a collection of disks embedded in an otherwise uniform host.
By the year 2000, the Postal Service aims to reduce its diesel transport by around 15000 vehicles. The advisory service, Detecon, is reviewing alternatives for postal deliveries as part of the ''Post 2000'' initiative. One such system is an electric-powered vehicle with a zinc-air battery from the Israeli firm Electric Fluid Limited (EFL). (orig.)
The variation of indole-3-acetic acid (IAA) transport along Lupinus albus L. hypocotyls was studied using decapitated seedlings and excised sections. To confirm that the mobile species...Full Text Available
An explicite PN solution of the multi-dimensional homogeneous neutron transport equation is given by expanding the angular flux into a series of geometry-independent spherical harmonics operators. An algorithm is developed for representing the spherical harmonic operators in orthogonal curvilinear coordinates. The general formulae are applied to two-dimensional spherical geometry; detailed P3 formulae are given. (orig.).
The purpose of the work reported in this paper was to measure the radionuclide buildup in primary heat transport system cell No. 3 at the Fast Flux Test Facility (FFTF) and to compare the results with predicted values from a model based on experimental studies and experience at similar reactors. The information obtained is used for maintenance planning and to enhance ability to assess radionuclide buildup in the future at FFTF and in other reactors.
This project (a) demonstrated specific dopamine transporter (DAT) uptake in vivo and metabolic stability of a radiolabelled cycloplentadieny rhenium compound in rats and baboons, (b) showed that cyclopentadieny tricarbonyl rhenium and technetium compounds conjugated tropanel could be made by metal transfer with ferrocenes; and (c) explored new methods of synthesizing these compounds under mild conditions.
Numerical errors of PALLAS calculation due to spatial mesh sizes are examined for a typical deep penetration shielding problem of isotropic incident fission neutrons penetrating a 200-cm-thick water slab. The exponential approximation for the source spatial distribution to solve the transport equation based on the direct integration method is verified to be effective for radiation transport in attenuating medium, while the linear approximation employed in the old PALLAS code is found to produce large errors for calculations with large mesh sizes.
In this study, we investigate an inter-industrial and inter-regional recycling system for industrial waste by the cement industry in Japan. We develop a linear programming model that represents cement production processes and waste transportation of all cement factories in Japan. We simulate cost and CO{sub 2}-minimizing systems. The result implies that making waste transportation more efficient in cost is an effective means for CO{sub 2} reduction. (author)
Microfluidic systems and methods are disclosed which are adapted to transport and lyse cellular components of a test sample for analysis. The disclosed microfluidic systems and methods, which employ an electric field to rupture the cell membrane, cause unusually rapid lysis, thereby minimizing continued cellular activity and resulting in greater accuracy of analysis of cell processes.
This paper reports a recent feasibility study of a scheme to transport thermal coal as a coal-water slurry by pipeline from Coalspur, Alberta to the west coast of Canada. The base case is for the transport of 10.2 million tons of coal annually, and an expanded case of 18.2 million tons is also considered. Coal would be drawn from 5 mines within a 16 km radius of Coalspur. Several different terminal layouts were considered.
The multidrug efflux transporter AcrAB-TolC is known to pump out a diverse range of antibiotics, including β-lactams. However, the kinetic constants of the efflux process, needed for the quantitative...Full Text Available
The membrane transport of the two stable lithium isotopes, {sup 6}Li and {sup 7}Li, by erythrocytes has been studied using a dual channel atomic absorption spectroscopic technique. {sup 6}Li appears to be taken up preferentially to {sup 7}Li, in the ratio of 10 to 40%, depending on the concentration of total lithium and on the lithium isotopic ratio in the external medium.
A modified version of the osmotic shock technique was used to investigate HCO3− and OH−transport in the alga Chara corallina. Cell...Full Text Available
This paper examines the technological, socio-economic and environmental feasibility of a proposal to strengthen the Italian commercial coastal shipping system as an alternative to bolstering existing road transport facilities in order to meet the requirements of increased trade in the new European free market. This prospect is of special importance to Italy due to its long coastlines. The principal elements taken into account are: investment capital, labour and energy requirements. The study first examines the supply and demand dynamics of the European transport system. Here, attention is given to energy consumption. This examination is followed by a review of the European situation with regard to normatives governing navigation and customs duties. Road, rail and maritime transport costs are compared, and an assessment is made of the economic feasibility of an integrated-means system of transport ...
The effects of purified Helminthosporium maydis T (HmT) toxin on active Ca/sup 2 +/ transport into isolated mitochondria and microsomal vesicles were compared for a susceptible (T) and a resistant (N) strain of corn (Zea mays). ATP, malate, NADH, or succinate could drive /sup 45/Ca/sup 2 +/ transport into mitochondria of corn roots. Ca/sup 2 +/ uptake was dependent on the proton electrochemical gradient generated by the redox substrates or the reversible ATP synthetase, as oligomycin inhibited ATP-driven CA/sup 2 +/ uptake while KCN inhibited transport driven by the redox substrates. Purified native HmT toxin completely inhibited Ca/sup 2 +/ transport into T mitochondria at 5 to 10 nanograms per milliliter while transport into N mitochondria was decreased slightly by 100 nanograms per milliliter toxin. Malate-driven Ca/sup 2 +/ transport in T mitochondria was ...
This report describes the 2005 version of the Fluka particle transport code. The first part introduces the basic notions, describes the modular structure of the system, and contains an installation and beginner's guide. The second part complements this initial information with details about the various components of Fluka and how to use them. It concludes with a detailed history and bibliography.
The effect of a number of dietary and synthetic chelating agents on the intestinal absorption of lead has been examined using the everted sac preparation and the tracer isotope /sup 203/Pb. All the agents examined increased lead transport into the serosal compartment. An explanation of the way in which these agents modify the model to describe lead transport is proposed.
The objective of this paper is to present a theoretical evaluation of soil and house related factors that may affect the transport of radon from the soil into houses. A two-dimensional mathematical model was used to simulate the diffusive and convective transport of radon into the house through idealized openings in the understructure. With the help of model predictions we tried to find out whether diffusion or convection predominates and under which circumstances. Radon transport through cracks in the house understructure is influenced mainly by the soil permeability, radon concentration at the soil-crack interface, the total area of cracks and the pressure difference across cracks. Because of its large range of variability, the soil permeability appears to have the greatest effect on the radon transport through cracks. At permeabilities below 1x10"-"1"2 m"2 diffusive transport ...
In research of nuclear medicine and development of radiopharmaceuticals, it is necessary to do data analysis and know transportation behaviors of the radionuclide in tissues or organs concerned. Based on polynomial fitting method, we developed the software INDFIT 1.0 programmed by Microsoft Visual Basic 6.0 (VB 6) for the data analysis. Experimental calculations showed that the software was of practical use
Recent experiments have explored the use of a free-electron laser (FEL) as a buncher for a microwave two-beam accelerator, and the subsequent driving of a standing-wave rf output cavity. Here the authors present a deeper analysis of the longitudinal dynamics of the electron bunches as they are transported from the end of the FEL and through the output cavity. In particular, the authors examine the effect of the transport region and cavity aperture to filter the bunched portion of the beam.
The effect of finite gyroradius on the current diffusive ballooning mode is examined. Starting from the reduced MHD equations including turbulent transports, coupling with drift motion and finite gyroradius effect of ions, we derive a ballooning mode equation with complex transport coefficients. The eigenfrequency, saturation level and thermal diffusivity are evaluated numerically from the marginal stability condition. Preliminary results of their parameter dependence is presented. (author)
A new nonlinear S{sub n} transport differencing scheme for slab geometry is presented that is fourth order accurate for small meshes and is strictly positive. The new scheme has been coded into the existing ONELD code and tested. Numerical results to demonstrate the accuracy and positivity of this new scheme are presented.
The bioenergetic capacity of skeletal muscle in a 17-year-old patient with a severe defect in complex III of the electron transport chain has been examined by 31P NMR measurements of the molar ratio...Full Text Available
Ammonia borane (AB) and sodium borohydride (SBH) are candidate materials for on-board hydrogen storage that can be dehydrogenated upon demand. The rheological properties of the dehydrogenated by-products are important to quantify their removal and transportability from the hydrogen storage system. This paper presents visco-elastic property (elastic stiffness and viscous damping) measurements of the spent fuels obtained from AB hydrolysis, hydrothermolysis and thermolysis; and SBH hydrolysis. Smaller stiffness and larger mobility (or smaller viscous damping) indicate better transportability of the spent fuel. In addition, flow property (dynamic angle of repose and avalanching time) measurements for the hydrolysis spent fuels of AB and SBH are also presented. Comparing with the SBH hydrolysis spent fuel, the AB hydrolysis spent fuel had a lower stiffness and larger mobility, as well as lower angles of dynamic repose and avalanche power peaks, ...
Several series of pyrimidine nucleosides were evaluated as part of a larger program to develop non-invasive brain imaging agents. The interaction of these antitumor/antiviral nucleosides with an NBMPR-sensitive murine erythroctye nucleoside transporter was evaluated by determining their inhibitory effect (K_i) on zero-trans influx of thymidine. Within each series of compounds, which had F, Cl, Br or I as halogen substituents, an increase in size of the halogen atom or a decrease in electronegativity decreased affinity for the transporter. Partition coefficients (P) of these pyrimidine nucleosides were measured to determine their potential to diffuse across the blood-brain-barrier (BBB). Most of the pyrimidine nucleosides had lower P values (log P < 0.9), and were considered to be poor candidates for simple diffusion across the BBB, although an active BBB transport mechanism for some nucleosides could be operative. For a ...
The transport properties of a 'thin' polycrystalline diamond film are analyzed after the sample exposure to 8.06-keV x-ray radiation. Structure and morphology of the as-grown film have been evaluated by Raman, x-ray diffraction, and scanning electron microscopy techniques. The transport properties have been investigated by measuring dark current-voltage characteristics in the temperature range of 60 to 360 K. Ohmic transport has been evidenced on the as-grown film up to 1.16x10"5 V/cm. After irradiation, nonlinear contributions to the dark current have been evidenced and related to field-assisted thermal ionization of traps. Below 200 K, hopping mechanisms have been observed. Correlations have been found among x-ray irradiation, density of traps involved in the transport processes, and the nonhomogeneous nature of the sample. A simple model of the grain boundary structure is proposed to explain the ...
The NRL Long Pulse Induction Linac is being employed in a Free Electron Laser (FEL) experiment. The authors present results of beam transport and focusing experiments as well as measurements of the output radiation generated by various magnetic wigglers. The electron gun of the accelerator presently has a 17-cmdiam. cold cathode which is located in a nearly zero magnetic field (B /SUB z/ less than or equal to 5 G). The gun voltage is flat to within approx. = + or - 5% for 1.5 #mu#sec with this graphite brush cathode. The beam is focused by a series of solenoidal coils as it propagates through the 4-m-long accelerator."2 A solenoidal field which can be varied from 1-10 kG confines the beam in the FEL interaction region. Previous experiments were limited by poor beam transport, focusing, and matching into the relatively large solenoidal field in the FEL region. By smoothing the axial magnetic field profile in the accelerator and making a more ...
Different concrete waste packages have been designed by Electricite de France (EDF) for the long-term storage of radioactive Low Level Waste (LLW). Their main function is to confine radionuclides from the biosphere for three hundred years in a near-surface disposal. According to the transport regulations, a Type-B package is needed for some waste like water filters. The water filters from EDF nuclear power plants are encapsulated in mortar and placed in a concrete container. Transport regulations for these containers have required the development of a methodology for safety assessment. The reference scenario of container degradation during transport considers a 9 m drop and a 800"oC fire for 30 min. First, the different chemical and physical processes involved in the containment of radionuclides are analysed. In particular, the radionuclide transport mechanisms in cement-based materials have been ...
Development of the engineering technology basis of pyrometallurgical reprocessing is a key issue for industrialization. For development of the transport technologies of molten salt and liquid cadmium at around 500 deg. C, a salt transport test rig and a metal transport test rig were installed in Ar glove box. Function of centrifugal pump and 1/2'' declined tubing were confirmed with LiCl- KCl molten salt. The transport behavior of molten salt was found to follow that of water. Function of centrifugal pump, vacuum sucking and 1/2'' declined tubing were confirmed with liquid Cd. With employing the transport technologies, industrialization applicable electro-refiner was newly designed and engineering-scale model was fabricated in Ar glove box. The electro-refiner has semi-continuous liquid Cd cathode instead of conventional one used in small-scale tests. With using actinide-simulating ...
A numerical model of multiphase air-water flow and contaminant transport in the unsaturated zone is presented. The multiphase flow equations are solved using the two-pressure, mixed form of the equations with a modified Picard linearization of the equations and a finite element spatial approximation. A volatile contaminant is assumed to be transported in either phase, or in both phases simultaneously. The contaminant partitions between phases with an equilibrium distribution given by Henry`s Law or via kinetic mass transfer. The transport equations are solved using a Galerkin finite element method with reduced integration to lump the resultant matrices. The numerical model is applied to published experimental studies to examine the behavior of the air phase and associated contaminant movement under water infiltration. The model is also used to evaluate a hypothetical design for a low-level radioactive waste disposal ...
A plan to diffuse instructional modules for the preparation of transportation professionals is presented, based on research and demonstration projects sponsored by the Urban Mass Transportation Administration (UMTA). The diffusion/adoption plan involves: developing instructional modules; disseminating the modules to professors teaching transportation courses at the college level; promoting the use and adoption of the modules; and evaluating and revising the plan. Transportation educators teach in a variety of disciplines (e.g., engineering, public administration, urban planning, and technology). Eight design and evaluation criteria for the instructional modules are listed, along with the procedures used by West Virginia University in developing the five instructional modules. The diffusion strategy involves repackaging UMTA research findings, change agents, and the use of information channels (e.g, ...
The coal transportation industry has played an integral role in determining coal markets, particularly in recent years, as the industry has evolved from cost-based pricing to market-based pricing. Aggressive pricing by Western rail carriers has caused extensive changes in coal marketing patterns in the Midwest. Multi-modal barge deliveries of Western and Appalachian coals have also played a significant, though lesser, role in changing coal markets. Eastern rail carriers have generally lagged in their aggressive pursuit of new coal markets and, to date, few changes in coal markets have been realised. The historic dominant role of transportation in shaping markets for coal will continue into the future as the utility industry contends with the effects of CAAA compliance and deregulation and as the coal industry contends with regional coal displacements and major imbalances in supply and demand. The dominance of rail ...
On July 18, 2001, a freight train carrying hazardous (non-nuclear) materials derailed and caught fire while passing through the Howard Street railroad tunnel in downtown Baltimore, Maryland. The United States Nuclear Regulatory Commission (USNRC), one of the agencies responsible for ensuring the safe transportation of radioactive materials in the United States, undertook an investigation of the train derailment and fire to determine the possible regulatory implications of this particular event for the transportation of spent nuclear fuel by railroad. Shortly after the accident occurred, the USNRC met with the National Transportation Safety Board (NTSB), the U.S. agency responsible for determining the cause of transportation accidents, to discuss the details of the accident and the ensuing fire. Following these discussions, the USNRC assembled a team of experts from the National Institute of Standards ...
This report documents a one-dimensional numerical model, SAMFT1D, developed to simulate single-phase and multiphase fluid flow and solute transport in variably saturated porous media. The formulation of the governing equations and the numerical procedures used in the code for single-phase and multiphase flow and transport are presented. The code is constructed to handle single-phase as well as two or three-phase flow conditions using two integrated sets of computational modules. The fully implicit scheme is used in the code for both single-phase and multiphase flow simulations. Either the Crank-Nicholson scheme or the fully implicit scheme may be used in the transport simulation. The single-phase modules employ the Galerkin and upstream weighted residual finite element techniques to model flow and transport of water (aqueous phase) containing dissolved single-species contaminants concurrently or ...
Durability of 28 days compressive strength concrete of 20 to 120 MPa has been studied. The ability of concrete to transport aggressive agents has been determined for four properties: the air permeability, the chloride diffusivity, the water absorption and the carbonation. A chloride migration test for high and very high strength concrete (HSC and VHSC) has been built. The relationship between transport properties and the compressive strength after one and 28 days of humid curing has always the same shape: transport decreases when strength increases. However, transport properties often vary in the ordinary concrete field. Beyond, the domain is much more limited. The relationship between transport properties and strength valid for ordinary concrete can not be simply extrapolated for HSC and VHSC. To determine the part of microcracking of HSC and VHSC, concrete behaviour stored in two ...
Volume V of the documentation for the Phase I Data Analysis Task performed in support of the current Regional Flow Model, Transport Model, and Risk Assessment for the Nevada Test Site Underground Test Area Subproject contains the transport parameter and source term data. Because of the size and complexity of the model area, a considerable quantity of data was collected and analyzed in support of the modeling efforts. The data analysis task was consequently broken into eight subtasks, and descriptions of each subtask's activities are contained in one of the eight volumes that comprise the Phase I Data Analysis Documentation.
Single-crystalline PbTe nanowires were synthesized using the chemical vapor transport method. They consisted of rock-salt structure PbTe nanocrystals uniformly grown in the [100] direction. We fabricated field-effect transistors using a single PbTe nanowire, providing evidence for its intrinsic n-type semiconductor characteristics. The values of the carrier mobility and concentration were estimated to be 0.83 cm"2 V"-"1 s"-"1 and 8.8 x 10"1"7 cm"-"3, respectively. The Seebeck coefficients (-72 ?V K"-"1) of individual nanowires were measured to show their n-type carrier-dominated thermoelectric transport properties.
Quark Gluon Plasma transport coefficients for heavy quarks and quark-antiquark pairs are computed through an extension of the results obtained for a hot QED plasma by describing the heavy-quark propagation in the eikonal approximation and by weighting the gauge field configurations with the Hard Thermal Loop effective action. It is shown that such a model allows to correctly reproduce, at leading logarithmic accuracy, the results obtained by other independent approaches. The results are then inserted into a relativistic Langevin equation allowing to follow the evolution of the heavy-quark momentum spectra. Our numerical findings are also compared with the ones obtained in a strongly-coupled scenario, namely with the transport coefficients predicted (though with some limitations and ambiguities) by the AdS/CFT correspondence.
Peculiarities of Kurchatov Institute WWR-2 and TR research reactors spent fuel treating and transportation for radiochemical processing are stated. Spent fuels were performed as fuel assemblies of different forms and containing similar fuel elements: EhK-10 with 10% enrichment UO2-Mg fuel kernels or S-36 with 36% enrichment U-Al alloys. Spent fuel storage conditions are described. Features of developed procedures for identification of fuel assemblies by type of fuel elements are given. Transport package TUK-19 for loading and transportation of spent fuel for processing was chosen. Details of spent fuel loading in TUK-19 that is conducted by personnel under protective sheet of water in special reclaim volume are described
For a soft-contact-lens (SCL) wearer, corneal health and comfort are strongly influenced by water transport through the polymeric materials used in lens fabrication. In particular, evaporative water loss at the anterior lens surface is a potential cause of contact-lens dehydration and of post-lens tear-film depletion, which in turn, may lead to discomfort, dryness syndrome, and/or lens adhesion.We present a solution-diffusion model for transport of water through soft-contact-lens materials to mimic evaporative dehydration from a contact lens during blinking and to access possible SCL adhesion to the corneal surface under a variety of environmental conditions (e.g., wind speed and relative humidity). To describe the water-transport process, we use an extended version of the Maxwell-Stefan m...
Analyses of pressure vessel damage resulting from neutron irradiation have primarily relied on two-dimensional transport calculations and a spatial-synthesis methodology to accommodate three-dimensional effects in the results of two two-dimensional calculations. In this paper, the authors report on calculations made on the Pool Critical Assembly (PCA) Benchmark, Configuration 12/13, using the three-dimensional, continuous energy Monte Carlo transport code, MCNP, and the three-dimensional, multigroup, diffusion accelerated discrete ordinates transport code THREEDANT. Neutron fluxes and activation rates as determined from these two calculations are compared to each other and to experimental results in the literature. The authors also draw some conclusions on the value of 3D calculations on the interpretation of experimental results.
The human erythrocyte (RBC) Rh blood group system consists of a complex of distinct integral membrane polypeptides with physical properties common to the aminophospholipid transporter responsible for the transbilayer movement of phosphatidylserine (PS) in RBC. To assess the involvement of Rh polypeptides in PS translocation, the aminophospholipid translocase was labeled with a photoactivatable PS analogue, "1"2"5I-azido-PS, and with an inhibitor of PS transport, "1"2"5I-labeled 2-(2-pyridyldithio)ethylamine. The ability of monoclonal Rh antibodies to immunoprecipitate the labeled transporter was determined. Immunoprecipitated Rh polypeptides were found to be labeled with the aminophospholipid translocase markers, suggesting that Rh proteins are involved in the transbilayer movement of PS.
Well over 90 percent of all motorized transportation is fueled by products of petroleum, but the end of such widespread use of oil may be in sight. Among alternative fuels for land transportation, electricity appears to be the most promising, in part because it can be renewably produced and readily transmitted. The inadequacy and high cost of on-board storage of electrical energy present major challenges to widespread deployment of electric traction, and may do so for many years. On-board generation of electrical energy also presents challenges. The third means of powering electric traction is to provide for connection to the electricity grid while in motion. Grid-connected vehicles (GCVs) are an established feature of many cities and of much inter-city transportation, chiefly as electric ...
Reactive chemical transport models developed over the past decade have generally relied on the assumption that local thermodynamic equilibrium is achieved at all times between aqueous species in a given system. Consequently, homogeneous aqueous systems characterized by a number of kinetically slow reactions, particularly problems involving organic species, cannot be satisfactorily modeled. In this study, we present a prototype computer model, KINETRAN, which is designed to handle kinetically-controlled homogeneous reactions in the aqueous phase, along with the transport of the various species involved, through geologic media. 31 refs., 53 figs., 10 tabs.
One of the major challenges facing the proposed high-energy linear e^+e^ colliders is the preservation of the extremely small vertical emittance from the damping rings to the interaction point (IP). This emittance must be transported through bunch compression sections, the main linac and finally through the beam delivery system to the IP. Historically, the beam dynamics issues of each subsystem have been studied quasi-independently, with the beam conditions and tolerances being specified at the boundaries. As part of the recent International Linear Collider Technical Review Committee, new simulation tools have been developed to simulate the beam transport through the integrated system, including static and dynamic errors, stabilization systems, and tuning algorithms.
Background, aim, and scope Semi-trailers with load boxes are the most important mode of grain transport by land in the world. Load boxes can be produced with different materials such as: wood, steel, and synthetic material. They are responsible for effectiveness retention and quality of grains during the transport. Among the main aspects to be considered and valued when selecting materials for load boxes are the final mass of the semi-trailer, loss of grains, and mechanical properties. Environmental performance is another important aspect to be taken into account for developing and selecting new materials in this kind of application. This study presents a comparative environmental evaluation of load boxes built from two different materials (a wood panel and a three-layer synthetic (TLS) pa...
The objective of this study is to describe the complete transport chain of CO2 between capture and storage including a ship transport. This last one is composed by the following steps:Shore terminal including the liquefaction, temporary storage and CO2 loading. Ship with a capacity of 30,000m^3. On or off shore terminal including an unloading system, temporary storage and export towards the final storage. Between all the possible thermodynamic states, the liquid one is most relevant two options are compared in the study (-50^oC, 7bar) and (-30^oC, 15bar). The ship has an autonomy of 6 days, is able to cover 1000km with a cargo of 2.5Mt/year. Several scenarios are studied varying the geographical position of the CO2 source, the number of harbours and the way the CO2 is finally stored. Depen...
Since the 1950s a transport revolution has occurred comparable to events in the late nineteenth century when sailing ships were replaced by steam vessels. Freight rates for bulk products have decreased 65-70% due to improved maritime technology, and formerly separate markets for bulk products have been unified globally. It is suggested that the low freight rates since the early 1980s will level out in future. Price peaks caused by political and military crises like the Korean war, the Suez crisis, OPEC I and OPEC II also seem to be of diminishing importance over time. The world market has become much less sensitive to external shocks due to alternative sources of supply and alternative transport routes. Examples are given from the coal, grain and iron ore international market. 62 refs., 24 figs., 4 tabs.
The solution of the governing steady transport equations for momentum, heat and mass transfer in flowing fluids can be very difficult. These difficulties arise from the nonlinear, coupled, nonsymmetric nature of the system of algebraic equations that results from spatial discretization of the PDEs. In this manuscript the authors focus on evaluating a proposed nonlinear solution method based on an inexact Newton method with backtracking. In this context they use a particular spatial discretization based on a pressure stabilized Petrov-Galerkin finite element formulation of the low Mach number Navier-Stokes equations with heat and mass transport. The discussion considers computational efficiency, robustness and some implementation issues related to the proposed nonlinear solution scheme. Computational results are presented for several challenging CFD benchmark problems as well as two large scale 3D flow simulations.
The solid particle movement in a rotary drum plays an important role in drying processes. The solid distribution in the drum affects the amount of contact surface between the solid and the gas. The retention time of solids influences the time particles can stay in contact with the gas in order to transfer heat and mass. Any heat and mass transfer model for a solid particle dryer must be able to predict solid flowrate and solid hold-up. There have been several reports in the literature regarding the modeling aspects of solid transport in dryers. If the model is developed for model-based control, it must be simple and yet represent dynamics of the system accurately. This paper addresses solid motion modeling and the effects of different variables involved in solid transport phenomena.
A controlled field pilot has been developed in Bozeman, Montana, USA, to study near surface CO2 transport and detection technologies. A slotted horizontal well divided into six zones was installed in the shallow subsurface. The scale and CO2 release rates were chosen to be relevant to developing monitoring strategies for geological carbon storage. The field site was characterized before injection, and CO2 transport and concentrations in saturated soil and the vadose zone were modeled. Controlled releases of CO2 from the horizontal well were performed in the summers of 2007 and 2008, and collaborators from six national labs, three universities, and the U.S. Geological Survey investigated movement of CO2 through the soil, water, plants, and air with a wide range of near surface detection techniques. An overview of these results will be presented.
Experiences in hazardous materials transportation show that purely strategic or planning approaches have considerable shortcomings and that real-time management must be addressed. The commercialization of advanced location and communications technology provides the basis for real-time guidance. Considering the possibilities this new technology offers, a real-time routing model is proposed. It is based on the premises that a human dispatcher must assess the impacts of sudden events on safety and transportation costs and evaluate safe and cost-effective routing alternatives. Due to high uncertainty and time constraints in this real-time environment, risks and transportation costs are expressed as preferences on an ordinal scale. The model has been integrated into a decision support system and assessed in an experimental setting. This ordinal preference model turned out to be superior to a utility approach due to its ...
A new numerical technique called the convolution-based particle tracking (CBPT) method is developed to simulate resident or flux-averaged solute concentrations in groundwater models. The method is valid for steady-state flow and linear transport processes such as sorption with a linear sorption isotherm and first-order decay. The CBPT method uses particle tracking to take advantage of the ability of particle-based approaches to maintain sharp fronts for advection-dominated transport problems common in groundwater modeling and because of the flexibility of the random walk method to simulate a wide range of possible forms of the dispersion tensor. Furthermore, the algorithm for carrying out the convolution and superposition calculation from particle tracking results is very efficient. We sho...
This paper presents results from a research case study that examined the distribution of travel time of origin-destination (OD) pairs on a transportation network under incident conditions. Using a transportation simulation dynamic traffic assignment (DTA) model, incident on a transportation network is executed under normal conditions, incident conditions without traveler information availability, and incident conditions assuming that users had perfect knowledge of the incident conditions and could select paths to avoid the incident location. The results suggest that incidents have a different impact on different OD pairs. The results confirm that an effective traveler information system has the potential to ease the impacts of incident conditions network wide. Yet it is also important to n...
Managing service quality is vital to retain customer satisfaction and augment revenues for any business organization. Often it is difficult to assess service quality due to lack of quantifiable measures and limited data. In this paper, we present a hybrid approach based on SERVQUAL and fuzzy TOPSIS for evaluating service quality of urban transportation systems. The proposed approach consists of three steps. The first step involves development of a SERVQUAL based questionnaire to collect data for measuring transportation service quality. The participants provide linguistic assessments to rate the service quality criteria and the alternatives. In step 2, the linguistic ratings are combined through fuzzy TOPSIS to generate an overall performance score for each alternative. The alternative wit...
The effect of TP (triosephosphates:glyceraldehyde-3 phosphate, GAP, +dihydroxyacetone phosphate, DHAP) on respiration, phosphorylation and matrix ATP/ADP ratios of isolated oat mesophyll mitochondria...Full Text Available
Meteorological and air-quality data, as well as surface tracer concentration values, were collected during 1990 to assess the impact of Navajo Generating Station (NGS) emissions on Grand Canyon National Park (GCNP) air quality. These data have been used in the present investigation to determine between direct and indirect transport routes taken by the NGS plume to produce measured high-tracer concentration events at GCNP. Results show that complex-terrain features affect local wind-flow patterns during winter in the Grand Canyon area. Local channelling, decoupled canyon winds, and slope and valley flows dominate in the region when synoptic systems are weak. Direct NGS plume transport to GCNP occurs with northeasterly plume-height winds, while indirect transport to the park is caused by wind direction shifts associated with passing synoptic systems. Calculated polluted airmass positions along the modeled streak lines match ...
During germination and early growth of castor bean (Ricinus communis), all cellular constituents of the endosperm are eventually transferred to the growing embryo. The present results...Full Text Available
Plastics debris in the marine environment, including resin pellets, fragments and microscopic plastic fragments, contain organic contaminants, including polychlorinated biphenyls (PCBs), polycyclic...Full Text Available
Strain F, a recently isolated ruminal bacterium, grew rapidly with glutamate or glutamine as an energy source in the presence but not the absence of Na. Monensin, a Na+/H+ antiporter, completely inhibited...Full Text Available
The Anglo-Danish programme of research on materials for advanced vehicular transport and other storage batteries is about to enter its fifth year. Here, the results of the work to date on solid electrolytes, electrode materials and cell design are reviewed and discussed as a systematic programme of cell testing gets underway.
High temperature thermochemistry of thoria-urania fuel for Advanced Heavy Water Reactor was investigated. Oxygen potential development within the matrix and distribution behaviors of the fission products (fps) in different phases were worked out with the help of thermodynamic and transport properties of the fps as well as fission generated oxygen and the detailed balance of the elements. Some of the necessary data for different properties were generated in this laboratory while others were taken from literatures. Noting the behavior of poor transports of gases and volatile species in the thoria rich fuel (thoria-3 mol% urania), the evaluation shows that the fuel will generally bear higher oxygen potential right from early stage of burnup, and Mo will play vital role to buffer the potential through the formation of its oxygen rich chemical states. The problems related to the poor transport and larger retention of fission ...
An overall hypothesis for benzene-induced leukemia is proposed. Key components of the hypothesis include a) activation of benzene in the liver to phenolic metabolites; b) transport of these metabolites...Full Text Available
The electronic band structure, transport properties, and lattice dynamics in AuX_2 (X = Al, Ga and In) under high pressure have been extensively studied with full potential linearized augmented plane wave and pseudopotential plane wave methods. The theoretical results for the electronic band structure and Fermi surface reveal pressure-induced electronic topological transitions (ETTs) in AuGa_2 and AuIn_2, while they are absent in AuAl_2, in excellent agreement with the experimental observations. Moreover, calculations of the transport properties at different pressures reveal subtle changes in the band structure close to the Fermi surface of the three intermetallic compounds. It is clear that the anomalies in transport properties are due to ETTs. Interestingly, a pressure-induced soft transverse acoustic (TA) phonon mode is identified only in AuGa_2. The TA phonon instability at the Brillouin zone boundary L point might be ...
The ratio of particle velocity to translation speed exceeds 1 for 98% of .... The westward movement of the meandering jet and eddy system transports nutrient- rich ...... J. Hydrometeor., 4, 1044-1066. Gedney, N., P. M. Cox, H. Douville, ...
The RADionuclide Transport, Removal, And Dose (RAD-TRAD) code is designed for U.S. Nuclear Regulatory Commission (USNRC) use to calculate the radiological consequences to the off-site population and to control room operators following a design-basis accident at light water reactor (LWR) power plants. This code utilizes updated reactor accident source terms published in draft NUREG-1465. The code will track the transport of radionuclides as they are released from the reactor pressure vessel, travel through the primary containment and other buildings, and are released to the environment. As the radioactive material is transported through the primary containment and other buildings, credit for several removal mechanisms may be taken, including sprays, suppression pools, overlying pools, filters, and natural deposition. Simple models are available for these different removal mechanisms that use, as input, information about the ...
The RADionuclide Transport, Removal, And Dose (RADTRAD) code is designed for US Nuclear Regulatory Commission (USNRC) use to calculate the radiological consequences to the offsite population and to control room operators following a design-basis accident at Light Water Reactor (LWR) power plants. This code utilizes updated reactor accident source terms published in draft NUREG-1465, ``Accident Source Terms for Light-Water Nuclear Power Plants.`` The code will track the transport of radionuclides as they are released from the reactor pressure vessel, travel through the primary containment and other buildings, and are released to the environment. As the radioactive material is transported through the primary containment and other buildings, credit for several removal mechanisms may be taken including sprays, suppression pools, overlying pools, filters, and natural deposition. Simple models are available for these different ...
BackgroundThe pregnane X receptor (PXR; NR1I2), a member of the nuclear receptor superfamily, regulates the expression of metabolic enzymes and transporters involved...Full Text Available
The Nuclear Regulatory Commission (NRC), working with the National Institute of Standards and Technology (NIST), Pacific Northwest National Laboratory (PNNL), and the National Transportation Safety Board (NTSB), performed analyses to predict the response of various spent fuel transportation cask designs when exposed to a fire similar to that which occurred in the Howard Street railroad tunnel in downtown Baltimore, Maryland on July 18, 2001. The thermal performance of three different spent fuel cask designs (HOLTEC HI-STAR 100, TransNuclear TN-68, and NAC-LWT) was evaluated with the ANSYSR and COBRA-SFS analysis codes, utilizing boundary conditions for the tunnel fire obtained using NIST's Fire Dynamics Simulator (FDS) code. NRC Staff evaluated the potential for a release of radioactive material from each of the three transportation casks analyzed for the Baltimore tunnel fire scenario. The results of these analyses are ...
SummaryThe life span of C. elegans can be increased via reduced function of the mitochondria; however, the extent to which mitochondrial alteration in a single,...Full Text Available
Cultural monitoring was used to study the incidence and sources of salmonellae in a 4160 bird broiler flock during the growing period, transport and processing in a commercial plant. No salmonellae...Full Text Available
Photosynthetic (Ps) electron transport pathways often contain multiple electron carriers with overlapping functions. Here we focus on two c-type cytochromes (cyt) in facultative phototrophic...Full Text Available
The erosion phenomena of coastal regions in Italy and in the world is remarkable from the environmental point of view. Are showed activities of mechanical defense and numerical models to solve complex problem.
It has been predicted that geometrically similar animals would swim at the same speed with stroke frequency scaling with mass−1/3. In the present study, morphological and behavioural...Full Text Available
The staff of the Spent Fuel Project Office at the U.S. Nuclear Regulatory Commission undertook the investigation and thermal analysis of the Baltimore tunnel fire event. This event occurred in the Howard Street tunnel, in Baltimore, Maryland, on July 18, 2001. The staff was tasked with assessing the consequences of this event on the transportation of spent nuclear fuel. This paper describes the staff's coordination with the following government and laboratory organizations: the National Transportation Safety Board (NTSB), to determine the details of the train derailment and fire; the National Institute of Standards and Technology (NIST), to quantify the thermal conditions within the tunnel; the Center for Nuclear Waste Regulatory Analysis (CNWRA), to validate the NIST evaluations, and the Pacific Northwest National Laboratory (PNNL), to assist in the thermal analysis. The results of the staff's review and analysis efforts are ...
Accumulation of certain nonmetabolizable sugar-phosphates (including α-methyl glucoside-6-phosphate) in Escherichia coli is growth inhibitory and elicits the glucose-phosphate...Full Text Available
Apolipoprotein (apo) A-IV is a polymorphic, intestinally derived apolipoprotein that is genetically linked to and similar in structure to apoA-I, the major apolipoprotein in high density lipoproteins...Full Text Available
This paper discusses the new quality assurance regulations of the Nuclear Regulatory Commission (NRC) for packaging and transportation of radioactive materials. These regulations became effective on October 18, 1977. Background information concerning these regulations and packaging and transportation history is included. The quality assurance program is described with indications of how it is composed of general (administrative) provisions which must meet the 18 quality assurance criteria and be approved by the NRC; specific provisions which appear in the DOT and NRC regulations and in the individual package design approval; and other specific procedures which are not required by regulations but which are necessary for the proper control of quality. The quality assurance program is to be developed using a graded approach for the application of pertinent criteria and optimizing the required degree of safety and control efforts involved in ...
Eukaryotic cells can massively transport their own cytoplasmic contents into a lytic compartment, the vacuole/lysosome, for recycling through a conserved system called autophagy. The key process in...Full Text Available
Technologies for materials, processes, and manufacturing that will provide safe, .... advanced energy conversion techniques, and pulsed inductive and ... duration antimatter storage and transportation, and methods for utilizing ...... EVA systems can be found in the EVA Technology Roadmap of the EVA Project Plan. ...
Combining in situ Rutherford backscattering and electrical transport measurements on low-temperature hydrogen-implanted amorphous Pd/sub 80/Si/sub 20/ films, we have studied the correlation between the hydrogen content and the resistivity.
Sucrose and mannitol are major photosynthetic products and translocates in celery. Assimilate partitioning and transport were studied by pulse-labeling leaves with /sup 14/CO/sub 2/ followed by different length chases in ambient air. After a 2 h chase in the light there was more /sup 14/C in sucrose than mannitol in source leaves and their petioles. In contrast after a 2 h dark chase leaves contained more /sup 14/C in mannitol than sucrose but petioles had more /sup 14/C in sucrose than mannitol. After a 15 h chase (6 h light; 9 h dark) labeled sucrose was higher in source petiole vascular bundles than in adjacent parenchyma tissue but label in glucose and fructose was higher in the parenchyma tissue. After the 15 h chase most of the /sup 14/C remaining in developing sink leaves and their petioles was in mannitol. Although in the light mannitol:sucrose ratios are the same in leaf and petiole tissues, in the dark sucrose is initially the major translocate with ...
... the provisions of NASA Handbook (NHB) 6000.1, Requirements for Packaging, Handling, and Transportation, and/or MIL-STD-2073-1 and MIL-STD-2073-2, ...
The Aer protein in Escherichia coli is a membrane-bound, FAD-containing aerotaxis and energy sensor that putatively monitors the redox state of the electron transport system. Binding...Full Text Available
May 1, 2011 ... Onboard Systems Record Unique Videos of Space Missions ... Corporation, An artist's rendering of LCROSS launching toward the Moon .... Station (ISS) as part of the Commercial Orbital Transportation Services program. ...
D-galactose, a monosaccharide rapidly phosphorylated within liver cells, is irreversibly removed from the portal circulation. We have studied the kinetic relations between the hepatic cell entry process...Full Text Available
In this study we use the multiple indicator dilution technique to outline the kinetic mechanisms underlying the uptake of rubidium, a cation which, in the steady state, is concentrated by hepatic parenchymal...Full Text Available
This report discusses the following topics on superconductivity: nonlinearities in hard superconductors such as surface impedance of a type II superconductimg half space and harmonic generation and intermodulation due to alternating transport currents; and nonlinearities in superconducting weak links such as harmonic generation by a long Josephson Junction in a superconducting slab.
at 4:30 P.M. November 30, 2006 - NTSB Sends Investigators to Metro Accident in Alexandria, Virginia November 27, 2006 - (SB-06-67) John Clark Assumes New Scientific Post at...
The polarity of biological mediums controls a host of physiological processes such as digestion, signaling, transportation, metabolism, and excretion. With the recent widespread use of near-infrared...Full Text Available
Klebsiella oxytoca can use nitrate and nitrite as sole nitrogen sources. The enzymes required for nitrate and nitrite assimilation are encoded by the nasFEDCBA operon....Full Text Available
considers a wide range of pathways to advance the nation's current capabilities. The present ... opment of new energy sources, aging infrastruc- ture and ... and manufacturing TA strategic roadmap as brief- ..... pellant storage and transport. ...
The mechanism of cytokinesis has been difficult to define because of the short duration and the temporal-spatial dynamics involved in the formation, activation, force production, and disappearance of...Full Text Available
Endosomal sorting complexes required for transport (ESCRTs) are heteromeric protein complexes required for multivesicular body (MVB) morphogenesis. ESCRTs I, II, III and III-associated are ubiquitous...Full Text Available
Oct 18, 2009... jumps mostly from C-130 and C-141 Air Force transports and Army UH-1H Huey helicopters with the "All American," 82nd Airborne Division. ...
In the presence of electrochemical energy, several branched-chain neutral and acidic amino acids were found to accumulate in membrane vesicles of Bacillus stearothermophilus. The membrane vesicles contained...Full Text Available
1 to the operator of a waste collective system by an agreement or contract. The conditions for the ...Fees of the waste collective system are subject to the supervision of the authority responsible for market surveillance. ...The operator of waste collection system collects wastes from producers and holder and transports these to a collection
Serial studies of iron transport in patients on maintenance dialysis showed normal or raised values in almost all subjects and a transient increase soon after the start of dialysis in three. These...Full Text Available
Simulations with a regional chemical transport model show that anthropogenic emissions of volatile organic compounds and nitrogen oxides (NOx = NO + NO2) lead to a dramatic diurnal...Full Text Available
Background and AimsXylem vessels containing gases (embolized) must be refilled with water if they are to resume transport of water through the plant, so refilling is of great importance...Full Text Available
A detailed molecular characterization of nuclear mRNA export will require an in vitro system, allowing a biochemical reconstitution of transport. To this end, an mRNA export assay has...Full Text Available
BackgroundMethamphetamine (METH), an addictive psycho-stimulant drug with euphoric effect is known to cause neurotoxicity due to oxidative stress, dopamine accumulation and glial...Full Text Available
Over 200 mutations in the retina specific member of the ATP-binding cassette transporter super-family (ABCA4) have been associated with a diverse group of human retinal diseases....Full Text Available
In 1983, the first modern urban transit bus powered by an alternative transportation fuel took to the roads in the USA. A little more than a decade later, about 2.5% of all USA buses are powered by alternative transportation fuels and about 20% of the new bus orders for delivery by 1998 will be powered by alternative fuels, mostly by natural gas. There are over 2,200 natural gas buses in operation or on order, plus about 600 additional buses powered by other alternative fuels. When these orders are completed, alternative fuel buses will constitute over 4.4% of the urban bus fleet in the USA. The widespread use of natural gas buses in the USA provides an important transition pathway for hydrogen vehicles because of the similarities between distribution, refueling and onboard energy storage technologies for the two fuels. Projects demonstrating advanced hybrid-electric buses powered by natural gas or hydrogen and projects demonstrating hydrogen ...
BACKGROUND: Although the importance of lymphatic function is well recognized, the lack of real-time imaging modalities limits our understanding of its role in many diseases. In a phase...Full Text Available
The major neuropathological correlate of cerebral palsy in premature infants is periventricular leukomalacia (PVL), a disorder of the immature cerebral white matter. Cerebral ischemia leading...Full Text Available
This book contains papers relating to the 1990 Future Transportation Technology Conference and Exposition. Included are the following papers; Examination of rich mixtures operation of a dual fuel engine, A hybrid natural gas vehicles, Performance analysis of a spark ignited engine with ETBE as a blending agent.
The publication presents the authentic texts of the: (1) Ordinance on road transport of hazardous materials (GGVS) with the ADR, as of 1993, skeleton ordinance, annexes A and B, reasons underlying the 4th ordinance amending the GGVS, directives for implementation, RS 002, instructions for accident management, RS-006, design approval standards for packaging materials and IBC-R002. (2) Ordinance on exemptions under the GGVS (GGAV). (3) Guiding principles for the training of vehicle drivers. (4) Catalogue of monetary fines under the GGVS, BKatV. (5) Draft version of catalogue of on-the-spot cautionary fines. (6) List of materials. (7) Technical rules TR IBC 003, non-electrical equipment, TRS 003, TRS 004, TRS 005, TRS 006. (HP).
PurposeTo investigate the effects of laser photocoagulation (LP)-induced ocular hypertension (OHT) on the survival and retrograde axonal transport of retinal ganglion cells (RGC),...Full Text Available
Cultural monitoring was used to determine the incidence and sources of salmonellae in a 4160-bird broiler flock raised on litter in 32 pens. Twenty-five of the pens remained apparently free of salmonellae...Full Text Available
Field campaigns were conducted in 1981 and 1982/83 on the Dutch coast near Egmond. Measurements were made of surface elevations, water velocities and sediment concentrations in 3 to 8 surf zone locations and 2 to 5 offshore locations simultaneously. A des...
This study assesses the feasibility of removing the FFTF reactor vessel from its current location in the reactor cavity inside the Containment vessel to a transporter for relocation to a burial pit in the 200 Area.
Sandia was presented with an opportunity in 1993 to design containers for the long term storage and transport of fissile material. This program was undertaken at the direction of the US Department of Energy and in cooperation with Lawrence Livermore National Laboratory and Los Alamos National Laboratory which were tasked with developing the internal fixturing for the contents. The hardware is being supplied by Allied Signal Federal Manufacturing and Technologies, and the packaging will occur at Mason and Hangar Corporation`s Pantex Plant. The unique challenge was to design a container that could be sealed with the fissile material contents; and, anytime during the next 50 years, the container could be transported with only the need for the pre-shipment leak test. This required not only a rigorous design capable of meeting the long term storage and transportation requirements, but also resulted in development of a ...
A comparison of chlorophyll-a fluorescence in brown algae (Macrocystis integrifolia, Fucus vesiculosis), green algae (Scenedesmus obliquus, Ulva sp.)...Full Text Available
... ions. MATERIALS AND METHODS Cell Culture Culture of NT2/D1 cells was carried out as described previously (18, 19). NT2 cells were plated at a density of 2. ... ...
Based on in vitro rat and human hepatocyte uptake studies showing inhibition of warfarin uptake in the presence of the non-specific organic anion transporting polypeptide (OATP)...Full Text Available
The effects of events which occur prior to slaughter, such as loading, transport, and holding at an abattoir, on the prevalence of Campylobacter species, including Campylobacter...Full Text Available
SpoIIIE is an FtsK-related protein that transports the forespore chromosome across the Bacillus subtilis sporulation septum. We use membrane photobleaching and protoplast assays to...Full Text Available
The plant-parasitic nematode Heterodera schachtii stimulates plant root cells to form syncytial feeding structures which synthesize all nutrients required for successful nematode development....Full Text Available
BackgroundDiurnal fluctuation of bile acid (BA) concentrations in the enterohepatic system of mammals has been known for a long time. Recently, BAs have been recognized as signaling...Full Text Available
This report describes accomplishment of simulations of Pyrochemical Process Operation by using virtual engineering models. The pyrochemical process using molten salt electrorefining would introduce new technologies for new fuels of particle oxide, particle nitride and metallic fuels. This system is a batch treatment system of reprocessing and re-fabrication, which transports products of solid form from a process to next process. As a results, this system needs automated transport system for process operations by robotics. In this study, a simulation code system has been prepared, which provides virtual engineering environment to evaluate the pyrochemical process operation of a batch treatment system using handling robots. And the simulation study has been conducted to evaluate the required system functions, which are the function of handling robots, the interactions between robot and process equipment, and the time schedule of process, in the ...
Details of ion transporting abnormalities in cystic fibrosis airway epithelium are now known. The central hypothesis, that excessive drying of the airway surfaces is a primary event that leads to all...Full Text Available
ATP-binding cassette A3 (ABCA3) is a lipid transport protein required for synthesis and storage of pulmonary surfactant in type II cells in the alveoli. Abca3 was conditionally deleted...Full Text Available
Pesticide chemicals are an important component of modern agriculture. Through their use, plants and animals are exposed to pesticides directly and indirectly from transport through soil, water, and...Full Text Available
Phosphorus is an essential mineral that maintains cellular energy and mineralizes the skeleton. Because complex actions of ion transporters and regulatory hormones regulate serum phosphorus concentrations,...Full Text Available
The development of modern techniques for the cooling and the manipulation of atoms in recent years, and the possibility to create Bose-Einstein condensates and degenerate Fermi gases and to load them into regular optical lattices or disordered optical potentials, has evoked new interest for the disorder-induced localization of ultra-cold atoms. This work studies the transport properties of matter waves in disordered optical potentials, which are also known as speckle potentials. The effect of correlated disorder on localization is first studied numerically in the framework of the Anderson model. The relevant transport parameters in the configuration average over many different realizations of the speckle potential are then determined analytically, using self-consistent diagrammatic perturbation techniques. This allows to make predictions for a possible experimental observation of coherent transport phenomena for cold atoms ...
Feb 15, 2008 ... hydrological properties (e.g., hydrometeor budgets, cloud ice/water, transports ... cloud fraction, mass and particle size. In combination, these .... anomaly in the LS is not simply due isentropic movement of water vapor relative ...
Cytokinins are distributed through the vascular system and trigger responses of target cells via receptor-mediated signal transduction. Perception and transduction of the signal can occur at the plasma...Full Text Available
3-["1"2"3I]Iodo-L-#alpha#-methyl tyrosine (["1"2"3I]IMT) scintigraphy of extracranial malignant tumors has been described, but little is known about the transport systems involved in ["1"2"3I]IMT uptake into extracranial tumor cells. Here, the precise kinetics of ["1"2"3I]IMT transport into human Ewing's sarcoma cells (VH-64) was determined. The apparent Michaelis constant was of high affinity value (K_m=41.7#+-#3.9 #mu#M) and maximum transport velocity amounted to V_m_a_x=20.7#+-#0.6 nmol#centre dot#mg protein"-"1#centre dot#10 min"-"1. Inhibition experiments revealed the predominance of ["1"2"3I]IMT uptake via sodium-independent system L.
The treatment of brain disorders is one of the greatest challenges in drug delivery because of a variety of main barriers in effective drug transport and maintaining therapeutic concentrations in the...Full Text Available
DYF-13, originally identified in C. elegans within a collection of dye-filling chemosensory mutants, is one of several proteins that have been classified as putatively involved...Full Text Available
A bedload trap in the riverbed provided direct quantitative measurement of debris-transport rate in the East Fork River, Wyoming, a basin of 466 km2 drainage area. Traction load moves only...Full Text Available
Recent studies suggest that calcium influx via L-type calcium channels is necessary for psychostimulant-induced behavioral sensitization. In addition, chronic amphetamine upregulates subtype...Full Text Available
Indole-3-acetyl-myo-inositol occurs in both the kernel and vegetative shoot of germinating Zea mays seedlings. The effect of a gravitational stimulus on the transport...Full Text Available
Background and aims: Serotonin (5-hydroxtryptamine, 5-HT) is an important factor in gut function, playing key roles in intestinal peristalsis and secretion, and in sensory...Full Text Available
The Federal Highway Administration (FHWA) has put a high priority on the use of existing dynamic message signs (DMS) to provide travel time estimates to the public. The Oregon Department of Transportation (ODOT) has three DMS in the Portland metropolitan ...
Antisense radiopharmaceuticals could be used to image gene expression in the brain in vivo, should these polar molecules be made transportable through the blood–brain barrier....Full Text Available
The search for alternative fuels and new fuel resources is a top priority for Turkey, as is the case in the majority of countries throughout the world. The fuel policies pursued by governmental or civil authorities are of key importance in the success of alternative fuel use, especially for widespread and efficient use. Following the 1973 petroleum crisis, many users in Turkey, especially in transportation sector, searched for alternative fuels and forms of transportation. Gasoline engines were replaced with diesel engines between the mid-1970s and mid-1980s. In addition, natural gas was introduced to the Turkish market for heating in the early 1990s. Liquid petroleum gas was put into use in the mid-1990s, and bio-diesel was introduced into the market for transportation in 2003. However, after long periods of indifference governmental action, guidance and fuel policies were so weak that they did not make sense. ...
Albumin is a multifunctional transport protein that binds a wide variety of endogenous substances and drugs. Insulins with affinity for albumin were engineered by acylation of the epsilon-amino group...Full Text Available
Acquired resistance to the action of insulin to stimulate glucose transport in skeletal muscle is associated with obesity and promotes the development of type 2 diabetes. In skeletal muscle,...Full Text Available
The endoplasmic reticulum (ER) is an essential cellular compartment responsible for Ca2+ sequestration, signaling, protein translation, folding as well as transport. Several acute...Full Text Available
The report contains an overall frame for transformation of knowledge and experience from risk analysis to emergency education. An accident model has been developed to describe the emergency situation. A key concept of this model is uncontrolled flow of energy (UFOE), essential elements are the state, location and movement of the energy (and mass). A UFOE can be considered as the driving force of an accident, e.g., an explosion, a fire, a release of heavy gases. As long as the energy is confined, i.e. the location and movement of the energy are under control, the situation is safe, but loss of confinement will create a hazardous situation that may develop into an accident. A domain model has been developed for representing accident and emergency scenarios occurring in society. The domain model uses three main categories: status, context and objectives. A domain is a group of activities with allied goals and elements and ten specific domains have been investigated: process plant, ...
Biological membranes are lamellar structures composed of two leaflets capable of supporting different mechanical stresses. Stress differences between leaflets were generated during micromechanical experiments...Full Text Available
BackgroundSLC19A3 (solute carrier family 19, member 3) is a thiamin transporter with 12 transmembrane domains. Homozygous or compound heterozygous mutations in SLC19A3 cause...Full Text Available
In the cyanobacterium Anabaena sp. strain PCC 7120, open reading frames (ORFs) alr3026, alr3027, and all3028 encode a tripartite ATP-independent...Full Text Available
...Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION QUALIFICATION AND CERTIFICATION OF LOCOMOTIVE ENGINEERS Component Elements of the Certification Process ยง 240.109 General criteria for eligibility based on...
...Acquisition Regulations System 3 2010-10-01...Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF...assumption of risk for transportation by conveyance on...Missiles, and Space Launch Vehicles,...
On July 18, 2001, a freight train carrying hazardous (non-nuclear) materials derailed and caught fire while passing through the Howard Street railroad tunnel in downtown Baltimore, Maryland. The United States Nuclear Regulatory Commission (USNRC), one of the agencies responsible for ensuring the safe transportation of radioactive materials in the United States, undertook an investigation of the train derailment and fire to determine the possible regulatory implications of this particular event for the transportation of spent nuclear fuel by railroad. Shortly after the accident occurred, the USNRC met with the National Transportation Safety Board (NTSB, the U.S. agency responsible for determining the cause of transportation accidents), to discuss the details of the accident and the ensuing fire. Following these discussions, the USNRC assembled a team of experts from the National Institute of Standards ...
An algorithm is proposed which treats the ECH generated potential in a self-consistent way, by extending the Monte-Carlo Fokker-Planck method used by Murakami [S. Murakami et al., Proc. 17th IAEA Fusion Energy Conference, Yokohama, 1998 (International Atomic Energy Agency, Vienna, in press), paper CN-69/TH2/1]. The additional physics is expected to influence the transport of both thermal and suprathermal electrons in a helical toroidal system. (author)
The product of several advanced physical coal cleaning processes is a dry, ultrafine coal (DUC), in the order of 10 microns mean mass diameter. To utilize this fuel commercially, cost-effective, environmentally safe systems must be provided for the storage, transport, and handling of this finely divided form of fuel. The objective of the project described herein is the development of total logistics systems for DUC, including experimental verification of key features. The systems to be developed will provide for safe, economic, and environmentally protective storage and delivery of DUC for residential, commercial, and industrial uses. 20 figs.
Impurity effects on the photon-assisted spin-polarized transport through armchair carbon nanotubes connected with ferromagnetic leads are investigated theoretically. The impurity induces one resonant state whose position depends on the impurity strength, which can break the electron-hole symmetry. Whether the impurity suppresses or enhances the spin-coherent current depends on the nanotube length. When the microwave fields are applied on the nanotube, additional small side peaks caused by the photon-assisted tunneling are found. With increasing the impurity strength, one new current peak appears under the influence of both the microwave fields and the impurity.
This paper presents the objective, scope, and status of the U.S. Department of Energy's Materials and Structures Program to develop a data base on mechanical properties of structural materials for out-of-core structures and components for LMFBRs. Information on the development of a reference data base on materials for the reactor system, reactor enclosure system, primary heat transport system, intermediate heat transport system, and steam generator system is included. In addition, the development of the data and analyses to account for the effects of temperature and stress, as well as water/steam, sodium, and radiation environments, is described. Plans for the development of alternative materials for future out-of-core applications are presented. (author).
The performance of a high temperature ion source coupled to a helium gas-jet transport system for an efficient mass separation of neutron-rich alkaline earth and lanthanide isotopes is reported and the results of overall efficiency measurements using different cluster materials in the gas-jet are given. A fast, microprocessor controlled tape transport system for ..gamma..-spectroscopic studies on short-lived isotopes is described. Some results on the decay of 3.8sub(-s) /sup 152/Pr are presented. (orig.).
The performance of a high temperature ion source coupled to a helium gas-jet transport system for an efficient mass separation of neutron-rich alkaline earth and lanthanide isotopes is reported and the results of overall efficiency measurements using different cluster materials in the gas-jet are given. A fast, microprocessor controlled tape transport system for ..gamma..-spectroscopic studies on short-lived isotopes is described. Some results on the decay of 3.8sub(-s) /sup 152/Pr are presented.
We study diffusion of Cosmic Rays (CRs) in turbulent magnetic fields using test particle simulations. Electromagnetic fields are produced in direct numerical MHD simulations of turbulence and used as an input for particle tracing, particle feedback on turbulence being ignored. Statistical transport coefficients from the test particle runs are compared with earlier analytical predictions. We find qualitative correspondence between them in various aspects of CR diffusion. In the incompressible case, that we consider in this paper, the dominant scattering mechanism occurs to be the non-resonant mirror interactions with the slow-mode perturbations. Perpendicular transport roughly agrees with being produced by magnetic field wandering.
The validation of the forecast model for early emergency response to nuclear accidents is evaluated by trace tests in atmosphere in Daya Bay nuclear power site. The simulation experiment of the Daya Bay nuclear power site shows that the particle spreading image and the time-integrated concentration distribution given by plume concentration prediction model can perform the variation of pathway of the pollutant transport, as well as the effects of topography on transport and diffusion of pollutants. The simulation of five trace tests in field shows that 59.1% of ratios between predicted results and observed results are within the range of 10, and 41% of ratios are within the range of 5 approximately. (authors)
The author briefly discusses definition of terms, gives an introduction to measurement techniques and describes the characteristics of various low-frequency fields and their causes using typical examples: natural electric fields (thunderstroms), natural magnetic fields, technical electric constant fields (urban transportation, households), static magnetic fields (urban transportation, nuclear magnetic resonance imaging), technical electric alternating fields (high-voltage transmission lines, households), and magnetic alternating fields (high-voltage transmission lines). The author discusses both occupational exposure and that of the general public while underpinning his statements by numerous tables, measurement diagrams and charts. (Uhe).
Multi-machine experimental observations indicate resilience in the temperature profiles at low pedestal temperatures,whereas at high pedestal temperatures the profile stiffness seems to disappear.The change of the profile behavior impacts the energy confinement,basically due to a strong non-linear dependence of the energy transport on the pedestal temperature together with different critical conditions for the onset of turbulence in the ions and electrons.This possible explanation for the different observations is based on the assumption that both ion and electron energy transport is governed by turbulence which sets in at a critical temperature gradient as well as on a significant energy equipartition between electrons and ions.
We have designed a new nucleobase, benzodeazaadenine (BDA) that has a stronger charge transport ability than guanine and is not destroyed during charge transport process. By incorporating this new nucleobase into DNA, we demonstrated a protocol for real DNA nano-wire that is far superior to natural DNA. We also demonstrated that the selectivity for the interaction of Mn(II) ion with guanine N7 in G runs is a HOMO-controlled process, and as a consequence, the selectivity for G-metal ion interactions obtained by 15N-NMR studies would directly reflect the HOMO distribution of G-containing sequences in B-DNA. PMID:12903077
The purpose of this project is to integrate a variety of geographic information systems capabilities and telecommunication technologies for potential use in geographic network and visualization applications. The specific technical goals of the project were to design, develop, and simulate the components of an audio/visual geographic communications system to aid future real-time monitoring, mapping and managing of transport vehicles. The system components of this feasibility study are collectively referred to as a Geographic Visualization and Communications System (GVCS). State-of-the-art techniques will be used and developed to allow both the vehicle operator and network manager to monitor the location and surrounding environment of a transport vehicle during shipment.
An analysis of the current state of track-laying operations in open-cut mines is given and their labor intensive nature is noted. The lag in the technological development of mechanization equipment for track laying and repair operations is noted. Results are given from developments by the Scientific Research Institute of Open-Pit Mining Operations in the field of mobile transportation technology and promising modular route design. Also examined are the problems of improving the routing bases in the industry. The absence of factories for manufacturing transportation technology and equipment is reflected most evidently in the technical and cost characteristics of coal production by an open-cut method and requires immediate solution.
In this short communication, we discuss the energy consumption trends in the Malaysian road transport sector, with a special emphasis on the energy losses due to vehicle aerodynamic drag on highways. The recent trends of energy consumption in the Malaysian road transport sector are reviewed. It is evidently shown that the aerodynamic losses represented exceed 1.2MTOE annually since 2002. A novel concept of vertical-axis wind turbine (VAWT) farms for harvesting aerodynamic energy losses on Malaysian highways is preliminarily proposed. The novel concept aims at providing a sustainable and green energy source for the lighting of the highway network in the country.