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Sample records for aaps

  1. Tectonics wins AAP Award

    AGU's newest journal, Tectonics, won the 1983 award for excellence in journal design and production given by the Association of American Publishers, Inc. (AAP), in the eighth annual professional and scholarly publishing awards competition. Edited by John F. Dewey, the bimonthly journal is a joint publication of AGU and the European Geophysical Society. Paul E. Tapponnier is the European editor and B.C. Burchfiel is the North American editor. The journal is now in its third year of publication.

  2. AAP Updates Recommendations on Car Seats

    ... Size Email Print Share AAP Updates Recommendations on Car Seats Page Content Article Body Children should ride ... of approved car safety seats. Healthy Children Radio: Car Seat Safety Dennis Durbin, MD, FAAP, lead author ...

  3. Aminoalkylpyridines (AAPs), triazoline metabolite analogues, as anticonvulsants highly effective in the MES test.

    Kadaba, Pankaja K; Dixit, Trupti

    2003-10-01

    Elucidation of the metabolism and pharmacology of 1,2,3-triazolines (TRs) led to the identification of the triazoline pharmacophore and the evolution of the aminoalkylpyridines (AAPs). The AAPs have no activity in the scMet test but are highly effective in the MES seizure test by the oral route. The AAPs bind to the sigma(1) receptor with low affinity, but high selectivity. They impair Glu release to the same extent as the triazolines and afforded a high degree of protection in the kindled rat. They show no affinity for the NMDA/PCP receptor sites; thus the toxic side effects of NMDA antagonists are absent in the sigma selective AAPs. Variations of the heterocyclic unit, the alkyl chain and the amino group in the AAP leads, indicated that the 4-pyridyl substituent along with a methyl (alkyl) group, and a 4-C1, 3-C1 or 3,4-C1(2) substitution on the N-phenyl group, afforded the most active compounds. Amino group modification by acylation did not improve activity. The hydrazone compounds were the most active. Although the AAPs are very effective in the MES and the kindling models of epilepsy, they showed only low to moderate activity in protecting neuronal cells in stroke-induced cerebral ischemia. In the case of the TR compounds, even the least effective TR afforded 47% protection from neuronal injury. It is not known at this point, whether activity in both the MES and scMet tests, which would imply a role for both Glu and GABA, is a prerequisite for antiischemic activity. PMID:12871088

  4. Inadequacies with the ACOG and AAP statements on managing ethical conflict during the intrapartum period.

    Chervenak, Frank A; McCullough, Laurence B

    1991-01-01

    [The American College of Obstetricians and Gynecologists] takes the view that respect for the autonomy of the pregnant woman should be almost absolute and [the American Academy of Pediatrics] takes the view that respect for the autonomy of the pregnant woman should be limited or prima facie in character. ACOG's reading of this central bioethical principle admits of virtually no exceptions, while AAP's reading does allow exceptions....A complete account of obstetric ethics that would apply in clinical practice during the intrapartum period must take into account both negative and positive autonomy-based rights of the pregnant woman and beneficence-based obligations to the at-term fetus -- and to the pregnant woman, as well. Such an account will be, we believe, more clinically applicable than the accounts offered by ACOG and AAP. PMID:11642910

  5. Connecting Source with Sink: The Role of Arabidopsis AAP8 in Phloem Loading of Amino Acids.

    Santiago, James P; Tegeder, Mechthild

    2016-05-01

    Allocation of large amounts of nitrogen to developing organs occurs in the phloem and is essential for plant growth and seed development. In Arabidopsis (Arabidopsis thaliana) and many other plant species, amino acids represent the dominant nitrogen transport forms in the phloem, and they are mainly synthesized in photosynthetically active source leaves. Following their synthesis, a broad spectrum of the amino nitrogen is actively loaded into the phloem of leaf minor veins and transported within the phloem sap to sinks such as developing leaves, fruits, or seeds. Controlled regulation of the source-to-sink transport of amino acids has long been postulated; however, the molecular mechanism of amino acid phloem loading was still unknown. In this study, Arabidopsis AMINO ACID PERMEASE8 (AAP8) was shown to be expressed in the source leaf phloem and localized to the plasma membrane, suggesting its function in phloem loading. This was further supported by transport studies with aap8 mutants fed with radiolabeled amino acids and by leaf exudate analyses. In addition, biochemical and molecular analyses revealed alterations in leaf nitrogen pools and metabolism dependent on the developmental stage of the mutants. Decreased amino acid phloem loading and partitioning to sinks led to decreased silique and seed numbers, but seed protein levels were unchanged, demonstrating the importance of AAP8 function for sink development rather than seed quality. Overall, these results show that AAP8 plays an important role in source-to-sink partitioning of nitrogen and that its function affects source leaf physiology and seed yield. PMID:27016446

  6. SELECTIVE EVALUATION OF TWO URINARY ENZYMES (NAG AND AAP BEFORE AND AFTER UNILATERAL SHOCK WAVE LITHOTRIPSY

    M. R. Nikoobakht

    2006-09-01

    Full Text Available Biological effects extracorporeal shock wave lithotripsy (ESWL is not precisely known. We have evaluated two urinary enzymes activity N-acetyl-B-D-glucosamine (NAG and alanine amino peptidase (AAP before and after unilateral ESWL as markers for renal parenchymal damage. Forty eight patients with kidney stones (mean age 39 who had presented for the first time or at least one year after their previous lithotripsy underwent ESWL. Urinary specimens were collected before and after first, third and seventh days of lithotripsy and NAG, AAP were evaluated. These enzymes displayed the greatest activity 24 hours after ESWL with significant difference compared to the control group, (P < 0.05 versus 0.02. Elevation of urinary enzymes activity correlated with stone size particularly stones larger than 2 cm. These data suggest that there is some tubular and parenchymal damage induced by ESWL that needs time to get improved. The higher urinary enzyme activity in patients with larger stones ( > 2 cm is probably related to injury resulting from passage of smaller stones, produced after lithotripsy of a large stone, and it is suggested that these patients are treated with a safer procedure.

  7. Synergistic Radiation Protective Effect of Purified Auricularia auricular-judae Polysaccharide (AAP IV with Grape Seed Procyanidins

    Haina Bai

    2014-12-01

    Full Text Available The aim of this study was to investigate the synergistic antioxidant potential and protective effect of grape seed procyanidins (GSP in combination with Auricularia auricular-judae polysaccharides (AAP IV on radiation injury in splenocytes. Rat splenocyte irradiation resulted in significantly higher apoptosis rate, malondialdehyde (MDA (p < 0.005, reactive oxygen species (ROS (p < 0.01; cell viability, total superoxide dismutase (T-SOD (p < 0.01, catalase (CAT (p < 0.01, glutathione peroxidase (GSH-PX (p < 0.05, activity and glutathione (GSH (p < 0.01 levels were significantly reduced, compared with the control group. “GSP + AAP IV” treatment of rat splenocytes at doses of “GSP (0.3 μg/mL + AAP IV (50 μg/mL” displayed higher radioprotective and antioxidative effects than the administration of either GSP or AAP IV, as evident by lower levels of MDA (p < 0.001 concentration, as well as higher cell viability and T-SOD (p < 0.05, CAT (p < 0.005, GSH-PX (p < 0.01 and GSH content compared to the radiation group. In addition, in vivo studies have shown that “GSP + AAP IV” significantly ameliorated the decrease of spleen index (p < 0.005 and spleen GSH (p < 0.005 levels and significantly inhibited the increase of MDA (p < 0.005 levels of spleen with radiation-induced damage, compared with the non-treated group. The in vivo and in vitro results suggested that GSP and AAP IV have a synergistic protective effect against radiation-induced injury by improving the antioxidant and immunomodulation activities.

  8. Construction and analysis of an intergeneric fusion from Pigmentiphaga sp. strain AAP-1 and Pseudomonas sp. CTN-4 for degrading acetamiprid and chlorothalonil.

    Wang, Guangli; Zhu, Danfeng; Xiong, Minghua; Zhang, Hui; Liu, Yuan

    2016-07-01

    Pseudomonas sp. CTN-4 degrades chlorothalonil (CTN) but not acetamiprid (AAP), and Pigmentiphaga sp. strain AAP-1 degrades AAP but not CTN. A functional strain, AC, was constructed through protoplast fusion of two parental strains (Pseudomonas sp. CTN-4 and Pigmentiphaga sp. strain AAP-1) in order to simultaneously improve the degradation efficiency of AAP and CTN. Fusant-AC with eight transfers on plates containing two antibiotics and CTN was obtained. For the purpose of identifying and confirming the genetic relationship between fusant-AC and its parents, randomly amplified polymorphic DNA (RAPD), scanning electron microscopy (SEM), and 16S ribosomal DNA (rDNA) analysis were performed. In toto, RAPD fingerprint analysis produced 194 clear bands with 9 primers, which not only had bands in common with strains CTN-4 and AAP-1, but also had its own novel fusant-specific bands. The genetic similarity indices between fusant-AC and parental strains CTN-4 and AAP-1 were 0.40 and 0.69, respectively. The result of SEM indicated that the cell morphology of fusant-AC differed from both its parents. The fusant strain AC possesses a strong capability for AAP and CTN degradation. At AAP concentration (50-300 mg L(-1)), the degradation was achieved within 5 h. At the initial dose of 50 and 100 mg L(-1) CTN, the percentages reached 96 and 91 % over a 36-h incubation period. The present study indicates that the protoplast-fusion technique may have possible applications in environmental pollution control. PMID:27023810

  9. Newborn Male Circumcision with Parental Consent, as Stated in the AAP Circumcision Policy Statement, Is Both Legal and Ethical.

    Brady, Michael T

    2016-06-01

    Newborn male circumcision is a minor surgical procedure that has generated significant controversy. Accumulating evidence supports significant health benefits, most notably reductions in urinary tract infections, acquisition of HIV and a number of other sexually transmitted infections, penile cancer, phimosis, paraphimosis, balanitis and lichen sclerosis. While circumcision, like any surgical procedure, has risks for complications, they occur in less than 1 in 500 infants circumcised and most are minor and require minimal intervention. The CDC and the American Academy of Pediatrics (AAP) believe that health benefits of circumcision outweigh the risks. For this reason, the AAP believes that parents should be allowed to make the decision concerning circumcision of their male infants after receiving non-biased information on health risks and health benefits. PMID:27338601

  10. The possibility of building nuclear power plant free from severe accident risk: PWR NPP with advanced all passive safety cooling systems (AAP SCS)

    A complete set of advanced all passive safety cooling systems (AAP SCS) for PWR NPP, actuated by natural force has been put forward in the article. Here the natural force mainly means the fore, which created by change of pressure distribution in the first loop of PWR as a result of operational regime conversion from one to another, including occurrence of accident situation. Correspondent safety cooling system will be actuated naturally and then put it into passive operation after occurring some kind of accident, so accidental situation will be mitigated right after it's occurrence and core residual heat will be naturally moved from the active core to the ultimate heat sink. There is no need to rely on automatic control system, any active equipment and human actions in all working process of the AAP SCS, which can reduce the probability of severe accident to zero, so as to exclude the need of evacuation plan around AAP nuclear power plant and eliminate the public's concern and doubt about nuclear power safety. Implementation of the AAP SCS concept is only based on use of evolutionary measures and state-of-the-art technology. So at present time it can be used for design of new-type third generation PWR nuclear power plant without severe accident risk, and for modernization of existing second generation nuclear power plant. (authors)

  11. AMC12和AAP1卫星更名加入新天星座

    华宇星

    2007-01-01

    2007年3月5日,SES新天卫星公司宣布,两颗SES系列通信卫星AMC 12(该卫星的33个转发器原提供给SES Astra公司使用,故也命名为Astra 4A)司AAP1已转交该公司运营,这两颗卫星分别重新命名为NSS10和NSS11,其所有用户及相关的容量管理业务现由SES新天卫星公司负责。基于此,现在新天卫星星座由7颗通信卫星组成,完善了全球不同地区间的连通性,以及除欧洲和北美地区之外世界上其它地区的容量分配服务及区域互联。

  12. RESPONSE SURFACE METHODOLOGY APPLIED TO LACCASES ACTIVITIES EXHIBITED BY STENOTROPHOMONAS MALTOPHILIA AAP56 IN DIFFERENT GROWTH CONDITIONS

    Said Galai,

    2011-12-01

    Full Text Available Stenotrophomonas maltophilia AAP56, laccase-producing bacteria, growing under different conditions, exhibit laccase activity that is highly affected by some environmental factors. Response surface methodology (RSM was applied for the determination of laccase factor dependence using two substrates: ABTS (2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid and DMP (2,6 Dimethoxy-phenol. RSM was carried out with a 24 factorial design using four variables, namely, dye (0 to 0.1 mg mL-1, Cu in Med (0 to 400 µM, shaking (0 to 150 rpm, and CuSO4 in assay (0 to 0.2 mM. Significant correlation between the effects of these variables on R1 (ABTS oxidase activity and R2 (DMP oxidase activity responses was detected. Astonishing results showed differences between these two activities with respect to copper activity dependence. Anoxic conditions exhibited a significant ability to induce the enzyme. This bacterial laccase activity (produced under optimal conditions according to RSM was used to decolorize an azoic dye, Reactive Black 5 (RB5. It was efficient only in the presence of a redox mediator to degrade RB5 after 20 min of incubation time.

  13. Cloning and preliminary functional analysis of an amino acid permease gene GmAAP2-like 1 in soybean%大豆氨基酸透性酶基因GmAAP2-like 1的克隆及功能初探

    李美美; 夏铜梅; 王丹; 梅圆圆; 王宁宁

    2016-01-01

    Amino acid permeases play important roles in transferring amino acids between different parts of the plants; however, so far their functions have rarely been studied in soybean. In this research, an amino acid per-mease gene,GmAAP2-like 1, was seen to be up-regulated in primary leaves of hydroponically grown soybean (Glycine max) cultivar ‘Williams 82’ under nitrogen-deficiency treatment compared with nitrogen-sufficient control. The RT-PCR analysis further showed thatGmAAP2-like 1 was much more abundantly in flowers, leaves and stems of soybean while it showed very low expression in roots and seeds. During the cloning pro-cess, it was found that the vector carrying35S:GmAAP2-like 1 could be transformed intoEscherichia colistrain DH5αsuccessfully but was lethal toAgrobacterium tumerfaciens strain GV3101. As an alternative strategy, this gene was then cloned by fusion PCR with an intron inserted after the ifrst extron and the following transforma-tions turned out to be successful in bothE. coli andAgrobacterium strains mentioned above. Subsequently, the obtainedArabidopsis thalianatransgenic lines of35S:GmAAP2-like 1 with intron were veriifed by genomic PCR and semi-quantitative RT-PCR analysis, and the results showed that the intron was cut off correctly and the gene of interest was overexpressed in almost all the lines examined. Furthermore, it was shown that even though the growth of wild-type and transgenic seedlings were both inhibited after toxic glutamate analogues treatment in comparison to that of the mock control, the growth inhibition of transgenic lines was much more signiifcant, implying that GmAAP2-like 1 could possibly transport such acidic amino acid.%氨基酸透性酶(amino acid permeases)在植物不同器官的氨基酸转运过程中发挥着重要作用,但迄今为止,大豆中氨基酸透性酶相关的研究甚少。本文利用大豆初生叶水培系统对大豆(Glycine max)品种‘Williams 82’进行缺氮处理后,发现与

  14. Perfil Nutricional Associado ao Índice de Obesidade de Idosos do Centro de Saúde Sebastião Pinheiro Bastos, AAP-VR, Volta Redonda – RJ

    Jair Antonio de Carvalho

    2009-03-01

    Full Text Available Trabalho autorizado pelo do Comitê de Ética do (UBM - 13/04/2006, tem como objetivo avaliar o estado nutricional e índice de obesidade de idosos voluntários do Centro de Saúde Sebastião Pinheiro Bastos, AAP-VR, Volta Redonda – RJ - 2006, através do levantamento do índice de massa corporal (IMC, (OMS-1995 e da circunferência de abdome (CA, (Han e cols-1995. Foram entrevistados 1952 idosos, de ambos os sexos, maiores de 60 anos. Obteve-se os seguintes resultados para a variável (CA, risco para as doenças cardiovasculares: nas mulheres, 526 (52,97% apresentaram CA > 88 cm e 467 (47,02% CA < 88 cm (sem risco para as doenças cardiovasculares; nos homens, 460 (76,79% CA < 102cm (sem risco e 139 (23,21% > 102cm (com risco. Quanto ao IMC, foram identificados 949 idosos (59,62% com IMC > 24,99Kg/m2 (sobrepeso, 611 (38,37% com peso normal e 32 (2,01% com baixo peso. Comparando os dados obtidos com os do PNSN, (Tavares e Anjos - 1989: o baixo peso 2,01% é inferior ao do PNSN 6,75%; o peso normal 38,37% é menor do que o nacional que é de 53,95%; o sobrepeso 59,61% é superior ao do PNSN que é de 40,30%. O sobrepeso é muito alto, contrastando com o baixo peso e peso normal, sinalizando possível vulnerabilidade para as doenças degenerativas e suas comorbidades.

  15. The spectral library of the AAPS

    Davie, Matthew; Tinney, Chris; Wittenmyer, Robert

    2015-08-01

    The Anglo-Australian Planet Search program has been operating since 1997 and is still collecting high-resolution spectra of more than two hundred solar-like stars. Over forty thousand stellar spectra have been collected through an Iodine cell, and the radial velocity of the target of each spectrum determined.This project aims to reduce and calibrate the spectra and remove the interposed Iodine spectrum in order to recover the original stellar spectrum. The resultant library of spectra, in addition to being made available to the wider astronomical community, will be used to examine stellar activity, and to create high signal-to-noise composite spectra.We present initial results of the reduction process and indicate the quality of composite spectra that may be expected.

  16. What Made Them Successful: An Introspective Survey of AAPS Members

    Pamela A. Rudnicki, BS

    2015-03-01

    Conclusion: The authors believe that recognizing the relative importance of such factors, and their associated biases, is essential for the process of selecting and developing future successful plastic surgeons.

  17. USA välispoliitiliste hoiakute muutumine XXI sajandi alguses / Aap Neljas

    Neljas, Aap, 1967-

    2003-01-01

    USA poliitikas on olnud ilmne tendents jagada "rahulikes piirkondades" korrahoidmise koormat teiste suurriikidega ja säilitada vabad käed suuremate kriiside lahendamiseks, kus kaalul on otseselt USA rahvuslikud huvid (nt ligipääs naftale või juhtroll maailmas)

  18. AAPS-FDA workshop white paper : Microdialysis principles, application and regulatory perspectives

    Chaurasia, Chandra S.; Mueller, Markus; Bashaw, Edward D.; Benfeldt, Eva; Bolinder, Jan; Bullock, Ross; Bungay, Peter M.; DeLange, Elizabeth C. M.; Derendorf, Hartmut; Elmquist, William F.; Hammarlund-Udenaes, Margareta; Joukhadar, Christian; Kellogg, Dean L.; Lunte, Craig E.; Nordstrom, Carl Henrik; Rollema, Hans; Sawchuk, Ronald J.; Cheung, Belinda W. Y.; Shah, Vinod P.; Stahle, Lars; Ungerstedt, Urban; Welty, Devin F.; Yeo, Helen

    2007-01-01

    Many decisions in drug development and medical practice are based on measuring blood concentrations of endogenous and exogenous molecules. Yet most biochemical and pharmacological events take place in the tissues. Also, most drugs with few notable exceptions exert their effects not within the bloods

  19. AAPS and US FDA Crystal City VI workshop on bioanalytical method validation for biomarkers.

    Lowes, Steve; Ackermann, Bradley L

    2016-02-01

    Crystal City VI Workshop on Bioanalytical Method Validation of Biomarkers, Renaissance Baltimore Harborplace Hotel, Baltimore, MD, USA, 28-29 September 2015 The Crystal City VI workshop was organized by the American Association of Pharmaceutical Scientists in association with the US FDA to continue discussion on the bioanalysis of biomarkers. An outcome of the Crystal City V workshop, convened following release of the draft FDA Guidance for Industry on Bioanalytical Methods Validation in 2013 was the need to have further discussion on biomarker methods. Biomarkers ultimately became the sole focal point for Crystal City VI, a meeting attended by approximately 200 people and composed of industry scientists and regulators from around the world. The meeting format included several panel discussions to maximize the opportunity for dialogue among participants. Following an initial session on the general topic of biomarker assays and intended use, more focused sessions were held on chromatographic (LC-MS) and ligand-binding assays. In addition to participation by the drug development community, significant representation was present from clinical testing laboratories. The experience of this latter group, collectively identified as practitioners of CLIA (Clinical Laboratory Improvement Amendments), helped shape the discussion and takeaways from the meeting. While the need to operate within the framework of the current BMV guidance was clearly acknowledged, a general understanding that biomarker methods validation cannot be adequately depicted by current PK-centric guidelines emerged as a consensus from the meeting. This report is not intended to constitute the official proceedings from Crystal City VI, which is expected to be published in early 2016. PMID:26795584

  20. Cultural bias in the AAP's 2012 Technical Report and Policy Statement on male circumcision

    Frisch, Morten; Aigrain, Yves; Barauskas, Vidmantas;

    2013-01-01

    The American Academy of Pediatrics recently released its new Technical Report and Policy Statement on male circumcision, concluding that current evidence indicates that the health benefits of newborn male circumcision outweigh the risks. The technical report is based on the scrutiny of a large nu...

  1. De grijze aap: ouderen, communicatie en consumentengedrag vanuit het perspectief van de evolutie

    Sikkel, D.

    2010-01-01

    Lang geleden, voordat er mensen bestonden, bleven alle dieren vruchtbaar tot hun dood. De afgelopen vijf miljoen jaar hebben wij ons ontwikkeld tot de enige diersoort met een menopauze. Kennelijk was dit ergens goed voor; de natuur geeft deze onvruchtbare extra tijd van leven niet cadeau. Het nut van de vijftigplusser, en daarmee zijn natuurlijke gedrag, moet er dus zijn, maar het staat niet scherp op ons netvlies. Marketeers en communicatieprofessionals, die ook de oudere doelgroep in het ha...

  2. Aap, Noot, Mustafa...: Het effect van taalafstand en koloniaal verleden op leesprestaties

    Kooistra, J.P.; Ultee, W.C.; Pelzer, B.J.

    2008-01-01

    This study examines the effect of the distance between origin language and destination language on the reading skills of immigrant students, by building upon insights on the development of world languages, obtained from human population genetics. Lower-level research units are 1759 first-generation immigrants from PISA 2003, all aged fifteen or sixteen, and living in a country which has a Germanic national language. Higher-level research units are 13 destination countries and 46 origin countr...

  3. Aap, Noot, Mustafa…: Het effect van taalafstand en koloniaal verleden op leesprestaties

    Kooistra, Jan-Paul; Ultee, Wout; Pelzer, Ben

    2008-01-01

    Summary A is for apple, B is for Bushra: The influence of language distance and colonial experience on reading This study examines the effect of the distance between origin language and destination language on the reading skills of immigrant students, by building upon insights on the development of world languages, obtained from human population genetics. Lower-level research units are 1759 first-generation immigrants from PISA 2003, all aged fifteen or sixteen, and living in a country which ...

  4. Introduction: A welcome to the first special animal health issue of AAPS PharmSci

    Martinez, Marilyn; Soback, Stefan

    2002-01-01

    The goal of this special volume is to provide veterinary scientists with state-of-the art reviews in animal health and to inform human health scientists of the various challenges and collaborative opportunities associated with their animal health counterparts. The contributors are highly respected experts, providing invaluable insights into current issues and state-of-the-art advances within veterinary medicine.

  5. Emerging regenerative approaches for periodontal reconstruction: a systematic review from the AAP Regeneration Workshop.

    Lin, Zhao; Rios, Hector F; Cochran, David L

    2015-02-01

    More than 30 years have passed since the first successful application of regenerative therapy for treatment of periodontal diseases. Despite being feasible, periodontal regeneration still faces numerous challenges, and complete restoration of structure and function of the diseased periodontium is often considered an unpredictable task. This review highlights developing basic science and technologies for potential application to achieve reconstruction of the periodontium. A comprehensive search of the electronic bibliographic database PubMed was conducted to identify different emerging therapeutic approaches reported to influence either biologic pathways and/or tissues involved in periodontal regeneration. Each citation was assessed based on its abstract, and the full text of potentially eligible reports was retrieved. Based on the review of the full papers, their suitability for inclusion in this report was determined. In principle, only reports from scientifically well-designed studies that presented preclinical in vivo (animal studies) or clinical (human studies) evidence for successful periodontal regeneration were included. Hence, in vitro studies, namely those conducted in laboratories without any live animals, were excluded. In case of especially recent and relevant reviews with a narrow focus on specific regenerative approaches, they were identified as such, and thereby the option of referring to them to summarize the status of a specific approach, in addition to or instead of listing each separately, was preserved. Admittedly, the presence of subjectivity in the selection of studies to include in this overview cannot be excluded. However, it is believed that the contemporary approaches described in this review collectively represent the current efforts that have reported preclinical or clinical methods to successfully enhance regeneration of the periodontium. Today's challenges facing periodontal regenerative therapy continue to stimulate important research and clinical development, which, in turn, shapes the current concept of periodontal tissue engineering. Emerging technologies--such as stem cell therapy, bone anabolic agents, genetic approaches, and nanomaterials--also offer unique opportunities to enhance the predictability of current regenerative surgical approaches and inspire development of novel treatment strategies. PMID:25644297

  6. Particle size analysis: AAPS workshop report, cosponsored by the Food and Drug Administration and the United States Pharmacopeia

    Burgess, Diane J.; Duffy, Eric; Etzler, Frank; Hickey, Anthony J.

    2004-01-01

    The concepts of particle engineering and dosage form design have become dominant themes in pharmaceutical manufacturing. This trend is not simply a reflection of the development of new, more sophisticated manufacturing methods of particles or dispersed systems but also recognition of the importance of quality control even in more traditional manufacturing processes. However, the diversity of particle treatments, methods of particle size analysis, expression and interpretation of data, and pro...

  7. Sports Injury Prevention Tip Sheet

    ... Apply Advertise with AAP Advertising on AAP.org Advertising on AAP Journals & Publications AAP Mailing and eMail List Corporate Relationships Conflict of Interest and Industry Relations Sponsorships Corporate Friends of Children Fund Corporate Relationship ...

  8. Whole-genome sequence of a freshwater aerobic anoxygenic phototroph, Porphyrobacter sp. strain AAP82, isolated from the Huguangyan Maar Lake in Southern China

    Li, X.; Koblížek, Michal; Feng, F.; Li, Y.; Jian, J.; Zeng, Yonghui

    2013-01-01

    Roč. 1, č. 2 (2013). ISSN 2169-8287 R&D Projects: GA ČR GAP501/10/0221; GA MŠk(CZ) ED2.1.00/03.0110 Institutional support: RVO:61388971 Keywords : Porphyrobacter * Huguangyan Maar Lake Subject RIV: EE - Microbiology, Virology http://genomea.asm.org/content/1/2/e00072-13.full

  9. Draamateatris müüakse pähkleid, aga ükski ei taha osta / Priit Pedajas ; interv. Aap Egorov

    Pedajas, Priit, 1954-

    1999-01-01

    Eesti Draamateatris Madis Kõivu näidend "Kui me Moondsundi Vasseliga kreeka pähkleid kauplesime, siis ükski ei tahtnud osta", lavastaja Priit Pedajas, kunstnik Pille Jänes, osades Ita Ever, Andrus Vaarik, Ain Lutsepp, Rein Oja, Laine Mägi, Kersti Kreismann jt. Esietendus 9. juunil

  10. Talking to Kids and Teens About Social Media and Sexting

    ... Current Openings How to Apply Advertise with AAP Advertising on AAP.org Advertising on AAP Journals & Publications AAP Mailing and eMail ... on Facebook today?” “Any new chats recently?” “Anyone text you today?” Share a bit about your daily ...

  11. Interaction mechanism between 4-aminoantipyrine and the enzyme lysozyme

    4-Aminoantipyrine (AAP) is scarcely administered as a kind of analgesic drug because of the side effect. The residue of AAP in the environment poses a potential threat to human health. To evaluate the toxicity of AAP at the protein level, the effects of AAP on lysozyme were investigated using spectroscopic and molecular docking methods. Addition of AAP effectively quenched the intrinsic fluorescence of lysozyme. Static quenching of lysozyme by AAP revealed the formation of complex. After the inner filter effect was eliminated, the number of binding sites, the binding constant and the thermodynamic parameters were measured, and indicated that AAP can spontaneously bind with lysozyme through hydrophobic interactions with one binding site. Molecular docking results revealed that AAP bound into the enzyme active site and interacted with the Trp 62 and Trp 63 residues of lysozyme, which illustrated that the lysozyme activity was inhibited by AAP, in accordance with the results of the lysozyme activity experiment. Furthermore, the binding of AAP can result in demonstrable change of the conformation of lysozyme. This work is helpful for clarifying the molecular toxic mechanism of AAP in vivo. - Highlights: → This work established the binding mode of AAP with lysozyme in molecular level. → Mechanism was explored by multiple spectroscopic and molecular docking methods. → AAP can inhibit lysozyme activity and induce conformational changes in lysozyme.

  12. The interaction between 4-aminoantipyrine and bovine serum albumin: Multiple spectroscopic and molecular docking investigations

    4-Aminoantipyrine (AAP) is widely used in the pharmaceutical industry, in biochemical experiments and in environmental monitoring. AAP as an aromatic pollutant in the environment poses a great threat to human health. To evaluate the toxicity of AAP at the protein level, the effects of AAP on bovine serum albumin (BSA) were investigated by multiple spectroscopic techniques and molecular modeling. After the inner filter effect was eliminated, the experimental results showed that AAP effectively quenched the intrinsic fluorescence of BSA via static quenching. The number of binding sites, the binding constant, the thermodynamic parameters and binding subdomain were measured, and indicated that AAP could spontaneously bind with BSA on subdomain IIIA through electrostatic forces. Molecular docking results revealed that AAP interacted with the Glu 488 and Glu 502 residues of BSA. Furthermore, the conformation of BSA was demonstrably changed in the presence of AAP. The skeletal structure of BSA loosened, exposing internal hydrophobic aromatic ring amino acids and peptide strands to the solution.

  13. To Keep Teens Slim, Focus on Health Not Weight

    ... rather than worry about the number on the bathroom scale, the American Academy of Pediatrics (AAP) says. ... and take a look at their own lifestyle habits. The AAP recommended having family meals as often ...

  14. NCBI nr-aa BLAST: CBRC-CBRE-01-1547 [SEVENS

    Full Text Available CBRC-CBRE-01-1547 gb|AAP05763.1| notch-like transmembrane receptor LIN-12 [Caenorhabdit...is briggsae] gb|AAP05765.1| notch-like transmembrane receptor LIN-12 [Caenorhabditis briggsae] AAP05763.1 4e-83 60% ...

  15. NCBI nr-aa BLAST: CBRC-OANA-01-1968 [SEVENS

    Full Text Available CBRC-OANA-01-1968 gb|AAP13045.1| NADH dehydrogenase subunit 2 [Pseudochaenichthys georg...ianus] gb|AAP13046.1| NADH dehydrogenase subunit 2 [Pseudochaenichthys georgianus] AAP13045.1 0.17 23% ...

  16. NCBI nr-aa BLAST: CBRC-ATHA-05-0006 [SEVENS

    Full Text Available CBRC-ATHA-05-0006 gb|AAP45159.1| Plant viral-response family protein [Solanum bulboca...stanum] gb|AAP45189.1| Plant viral-response family protein [Solanum bulbocastanum] AAP45159.1 4e-13 23% ...

  17. 2013年美国儿科学会《儿童和青少年2型糖尿病诊治指南》解读%Interpretation of AAP Management of Tpye 2 Diabetes Mellitus in Children and Adolescents

    夏萍; 刘超

    2013-01-01

    For recent years,obesity and diabetes mellitus prevalence rates have been increasing year by year in children and adolescents.Treatment education and medication are not adequately available for the young patients.The first treatment guideline for children and adolescents has been published online in 2013 which provides the direction for practice.This article is comprehensive analysis of the part of guildeline,aiming to share with the issue-interested people.%近年来,儿童和青少年的肥胖与糖尿病发病率逐年增加,而多数对于T1DM及T2DM的教育与治疗药物并不适用于儿童和青少年患者.2013年,首个针对儿童与青少年新诊断T2DM管理指南在线发表,为临床诊疗提供了指导.本文对指南进行部分理解与说明,与同道共勉.

  18. Book Trade Research and Statistics. Prices of U.S. and Foreign Published Materials; Book Title Output and Average Prices: 2000 Final and 2001 Preliminary Figures; Book Sales Statistics, 2001: AAP Preliminary Estimates; U.S. Book Exports and Imports: 2001; Number of Book Outlets in the United States and Canada; Review Media Statistics.

    Sullivan, Sharon G.; Barr, Catherine; Grabois, Andrew

    2002-01-01

    Includes six articles that report on prices of U.S. and foreign published materials; book title output and average prices; book sales statistics; book exports and imports; book outlets in the U.S. and Canada; and review media statistics. (LRW)

  19. Arylazopyrazoles as Light-Responsive Molecular Switches in Cyclodextrin-Based Supramolecular Systems.

    Stricker, Lucas; Fritz, Eva-Corinna; Peterlechner, Martin; Doltsinis, Nikos L; Ravoo, Bart Jan

    2016-04-01

    A simple and high yield synthesis of water-soluble arylazopyrazoles (AAPs) featuring superior photophysical properties is reported. The introduction of a carboxylic acid allows the diverse functionalization of AAPs. Based on structural modifications of the switching unit the photophysical properties of the AAPs could be tuned to obtain molecular switches with favorable photostationary states. Furthermore, AAPs form stable and light-responsive host-guest complexes with β-cyclodextrin (β-CD). Our most efficient AAP shows binding affinities comparable to azobenzenes, but more effective switching and higher thermal stability of the Z-isomer. As a proof-of-principle, we investigated two CD-based supramolecular systems, containing either cyclodextrin vesicles (CDVs) or cyclodextrin-functionalized gold nanoparticles (CDAuNPs), which revealed excellent reversible, light-responsive aggregation and dispersion behavior. To conclude, AAPs have great potential to be incorporated as molecular switches in highly demanding and multivalent photoresponsive systems. PMID:26972671

  20. Temporal Changes and Altitudinal Distribution of Aerobic Anoxygenic Phototrophs in Mountain Lakes

    Čuperová, Zuzana; Holzer, Evelyn; Salka, Ivette; SOMMARUGA, RUBEN; Koblížek, Michal

    2013-01-01

    Aerobic anoxygenic phototrophs (AAPs) are bacteriochlorophyll a-containing microorganisms that use organic substrates for growth but can supplement their energy requirements with light. They have been reported from various marine and limnic environments; however, their ecology remains largely unknown. Here infrared epifluorescence microscopy was used to monitor temporal changes in AAPs in the alpine lake Gossenköllesee, located in the Tyrolean Alps, Austria. AAP abundance was low (103 cells m...

  1. Ambient air pollution associated with suppressed serologic responses to Pneumocystis jirovecii in a prospective cohort of HIV-infected patients with Pneumocystis pneumonia.

    Blount, R. J.; Djawe, K.; Daly, K. R.; Jarlsberg, L.G.; Fong, S.; Balmes, J; Miller, R F; Walzer, P. D.; Huang, L; International HIV-associated Opportunistic Pneumonias Study

    2013-01-01

    BACKGROUND: Ambient air pollution (AAP) may be associated with increased risk for Pneumocystis pneumonia (PCP). The mechanisms underlying this association remain uncertain. OBJECTIVES: To determine if real-life exposures to AAP are associated with suppressed IgM antibody responses to P. jirovecii in HIV-infected (HIV+) patients with active PCP, and to determine if AAP, mediated by suppressed serologic responses to Pneumocystis, is associated with adverse clinical outcomes. METHODS: We conduct...

  2. National trends in off-label use of atypical antipsychotics in children and adolescents in the United States.

    Sohn, Minji; Moga, Daniela C; Blumenschein, Karen; Talbert, Jeffery

    2016-06-01

    The objectives of the study were as follows: to examine the national trend of pediatric atypical antipsychotic (AAP) use in the United States; to identify primary mental disorders associated with AAPs; to estimate the strength of independent associations between patient/provider characteristics and AAP use. Data are from the National Ambulatory Medical Care Survey and the National Hospital Ambulatory Medical Care Survey. First, average AAP prescription rates among 4 and 18-year-old patients between 1993 and 2010 were estimated. Second, data from 2007 to 2010 were combined and analyzed to identify primary mental disorders related to AAP prescription. Third, a multivariate logistic regression model was developed having the presence of AAP prescription as the dependent variable and patient/provider characteristics as explanatory variables. Adjusted odds ratios (AORs) with associated 95% confidence intervals (CIs) were estimated. Outpatient visits including an AAP prescription among 4 to 18-year-old patients significantly increased between 1993 and 2010 in the United States, and over 65% of those visits did not have diagnoses for US Food and Drug Administration-approved AAP indications. During 2007 to 2010, the most common mental disorder was attention-deficit hyperactivity disorder, accounting for 24% of total pediatric AAP visits. Among visits with attention-deficit hyperactivity disorder diagnosis, those with Medicaid as payer (AOR 1.66, 95% CI 1.01-2.75), comorbid mental disorders (e.g., psychoses AOR 3.34, 95% CI 1.35-8.26), and multiple prescriptions (4 or more prescriptions AOR 4.48, 95% CI 2.08-9.64) were more likely to have an AAP prescription. The off-label use of AAPs in children and adolescents is prevalent in the United States. Our study raises questions about the potential misuse of AAPs in the population. PMID:27281081

  3. Secondhand smoke in combination with ambient air pollution exposure is associated with increased CpG methylation and decreased expression of IFN-gamma in T effector cells and Foxp3 in T regulatory cells in children

    Kohli, Arunima; Garcia, Marco A; Miller, Rachel L.; Maher, Christina; Humblet, Olivier; Hammond, S; Nadeau, Kari

    2012-01-01

    Abstract Background Secondhand smoke (SHS) and ambient air pollution (AAP) exposures have been associated with increased prevalence and severity of asthma and DNA modifications of immune cells. In the current study, we examined the association between SHS and AAP with DNA methylation and expression of interferon-gamma (IFN-γ) and forkhead box protein 3 (Foxp3) in T cell populations. Methods Subjects 7–18 years old were recruited from Fresno (high AAP; n ...

  4. Preparation and pharmaceutical evaluation of acetaminophen nano-fiber tablets: Application of a solvent-based electrospinning method for tableting.

    Hamori, Mami; Nagano, Kana; Kakimoto, Sayaka; Naruhashi, Kazumasa; Kiriyama, Akiko; Nishimura, Asako; Shibata, Nobuhito

    2016-03-01

    In this study, we developed nano-fiber-based tablets with acetaminophen (AAP; LogPow=0.51) for controlled-release delivery systems and evaluated in vitro drug dissolution and in vivo pharmacokinetics in rats. Nano-fibers made from methacrylic acid copolymer S (MAC; EUDRAGIT(®) S100) and containing AAP were prepared using a solvent-based electrospinning (ES) method. In vitro dissolution rate profiles of AAP showed tableting pressure-dependent decreases and pH-dependent increases. The results of tablet tracking by X-ray irradiation showed tablets based on MAC nano-fibers did not disintegrate in the upper intestinal lumen and had the properties of a long-term-acting tablet. In addition, the in vitro release profiles of AAP from nano-fiber tablets prepared by dissolving MAC with AAP (NFT), nano-fiber tablets prepared by adsorbing AAP to drug-free MAC nano-fibers (NFTadso), and tablets prepared by adsorbing half the amount of AAP to MAC nano-fibers containing the remaining amount of AAP (NFThalf) showed independent controlled-release aspects of AAP compared with physical mixture tablets (PMT). In vivo pharmacokinetic studies in rats after intraduodenal administration of 14mg/rat AAP in NFT, NFTadso, and NFThalf demonstrated that all these tablets based on MAC nano-fibers showed sustained-release profiles compared with PMT, and showed ultra-sustained release properties for AAP. These new tablets based on MAC nano-fibers did not disintegrate in the intestine in the lower pH region, and the tablets could regulate the release of AAP in a pH-dependent manner. The ES method is a useful technique to prepare nano-fibers and showed promising results as an oral delivery system for sustained-release regulation. PMID:26898420

  5. Characterization of a novel acylaminoacyl peptidase with hexameric structure and endopeptidase activity.

    Szeltner, Zoltán; Kiss, András L; Domokos, Klarissza; Harmat, Veronika; Náray-Szabó, Gábor; Polgár, László

    2009-08-01

    We have overexpressed in E. coli, purified and investigated the kinetic, thermodynamic and biophysical properties of an acylaminoacyl peptidase (AAP), from the thermophile Pyrococcus horikoshii (PhAAP). It was shown that the electrostatic environment of the catalytic site of PhAAP substantially influenced the pH dependence of the specificity rate constant (k(cat)/K(m)). However, 0.3 M NaCl, which depressed the electrostatic effects, simplified the complex pH-rate profile. The rate of formation of the enzyme-substrate complex (k(1)) was obtained from a non-linear Arrhenius plot. The lack of substrate leaving group effects indicated that k(1) is the rate determining step in the catalysis. DSC and CD measurements demonstrated that PhAAP displayed a stable structure in the catalytically competent pH range. It was shown that PhAAP is not just an acylaminoacyl peptidase, but it also has an endopeptidase activity and so differs from the mammalian AAPs. Size exclusion chromatography with PhAAP revealed a hexameric structure, which is unique among the known members of the prolyl oligopeptidase family that includes AAPs and suggests that its cellular function may be different from that of the dimeric AAP also found in the same organism. PMID:19303951

  6. Secondhand smoke in combination with ambient air pollution exposure is associated with increasedx CpG methylation and decreased expression of IFN-γ in T effector cells and Foxp3 in T regulatory cells in children

    Kohli Arunima; Garcia Marco A; Miller Rachel L; Maher Christina; Humblet Olivier; Hammond S; Nadeau Kari

    2012-01-01

    Abstract Background Secondhand smoke (SHS) and ambient air pollution (AAP) exposures have been associated with increased prevalence and severity of asthma and DNA modifications of immune cells. In the current study, we examined the association between SHS and AAP with DNA methylation and expression of interferon-gamma (IFN-γ) and forkhead box protein 3 (Foxp3) in T cell populations. Methods Subjects 7–18 years old were recruited from Fresno (high AAP; n = 62) and Stanford, CA (low AAP; n = 40...

  7. Chat, E-Mail, Web, and Chat Room Slang and Acronyms

    ... Shop AAP Find a Pediatrician Family Life Medical Home Health Insurance Pediatric Specialists Family Dynamics Adoption & Foster Care Communication & Discipline Types of Families Media Work & Play ...

  8. Cyberbullying

    ... Shop AAP Find a Pediatrician Family Life Medical Home Health Insurance Pediatric Specialists Family Dynamics Adoption & Foster Care Communication & Discipline Types of Families Media Work & Play ...

  9. Benefits of Limiting TV

    ... Shop AAP Find a Pediatrician Family Life Medical Home Health Insurance Pediatric Specialists Family Dynamics Adoption & Foster Care Communication & Discipline Types of Families Media Work & Play ...

  10. Chemoprotective effect of insulin-like growth factor I against acetaminophen-induced cell death in Chang liver cells via ERK1/2 activation

    The insulin-like growth factor (IGF) system and type-I IGF receptor (IGF-IR) signaling are involved in protecting against chemotherapeutic drug-induced cell death in human hepatoma cells. Acetaminophen (AAP) hepatotoxicity is the leading cause of liver failure, and the prevention of AAP-induced cell death has been the focus of many studies. We determined whether IGF-I could protect against AAP-induced cell death in Chang liver cells and investigated the protective mechanism. Based on the results of MTS assays, LDH release assays, Hoechst 33342 cell staining, and DNA fragmentation experiments, AAP induced cell death in a dose-dependent manner. According to Western blot analysis, treatment with AAP increased the level of poly(ADP-ribose) polymerase (PARP) fragments in cells compared with that in control cells; however, caspase-3, a critical signaling molecule in apoptosis, was not activated after AAP overdose. Moreover, combined treatment with AAP and IGF-I inhibited PARP cleavage, which was consistent with the ability of IGF-I to restore the level of glutathione (GSH) and cell viability in GSH and MTS assays, respectively. We investigated whether the protective effect of IGF-I against AAP cytotoxicity is related to the extracellular signal-related kinase ERK1/2, which is generally activated by mitogenic and proliferative stimuli such as growth factors. Compared with AAP treatment alone, IGF-I and AAP co-treatment increased ERK1/2 phosphorylation but inhibited PARP cleavage. Thus ERK1/2 activation is instrumental in the protective effect of IGF-I against AAP-induced cell death in Chang liver cells

  11. Increasing Availability to and Ascertaining Value of Asthma Action Plans in Schools through Use of Technology and Community Collaboration

    Hanson, Tabitha K.; Aleman, Martha; Hart, Lacey; Yawn, Barbara

    2013-01-01

    Background: Approximately 9% of school-aged children in the United States have asthma. Since 1997, the Asthma Action Plan (AAP) has been recommended as an asthma self-management tool for individuals with asthma. In the school setting, the use of the AAP has been primarily dependent on communication between the family and the school through a paper…

  12. Enzymuria in neonates receiving continuous intravenous infusion of gentamicin

    Colding, H; Brygge, K; Brendstrup, L;

    1992-01-01

    with non-treatment periods in the same newborn infant (33 infants). The same tendency applied to AAP. Newborn infants receiving continuous intravenous infusion of gentamicin were not found to be at greater risk of nephrotoxicity than those receiving intermittent gentamicin treatment, using NAG and AAP...

  13. Kinetic, Spectroscopic, and X-ray Crystallographic Characterization of the Functional E151H Aminopeptidase from Aeromonas proteolytica.

    Bzymek,K.; Moulin, A.; Swierczek, S.; Ringe, D.; Petsko, G.; Holz, R.

    2005-01-01

    Glutamate151 (E151) has been shown to be catalytically essential for the aminopeptidase from Vibrio proteolyticus (AAP). E151 acts as the general acid/base during the catalytic mechanism of peptide hydrolysis. However, a glutamate residue is not the only residue capable of functioning as a general acid/base during catalysis for dinuclear metallohydrolases. Recent crystallographic characterization of the D-aminopeptidase from Bacillus subtilis (DppA) revealed a histidine residue that resides in an identical position to E151 in AAP. Because the active-site ligands for DppA and AAP are identical, AAP has been used as a model enzyme to understand the mechanistic role of H115 in DppA. Substitution of E151 with histidine resulted in an active AAP enzyme exhibiting a k{sub cat} value of 2.0 min{sup -1}, which is over 2000 times slower than r AAP (4380 min{sup -1}). ITC experiments revealed that Zn{sup II} binds 330 and 3 times more weakly to E151H-AAP compared to r-AAP. UV-vis and EPR spectra of Co{sup II}-loaded E151H-AAP indicated that the first metal ion resides in a hexacoordinate/pentacoordinate equilibrium environment, whereas the second metal ion is six-coordinate. pH dependence of the kinetic parameters k{sub cat} and K{sub m} for the hydrolysis of L-leucine p-nitroanilide (L-pNA) revealed a change in an ionization constant in the enzyme-substrate complex from 5.3 in r-AAP to 6.4 in E151H-AAP, consistent with E151 in AAP being the active-site general acid/base. Proton inventory studies at pH 8.50 indicate the transfer of one proton in the rate-limiting step of the reaction. Moreover, the X-ray crystal structure of [ZnZn(E151H-AAP)] has been solved to 1.9 {angstrom} resolution, and alteration of E151 to histidine does not introduce any major conformational changes to the overall protein structure or the dinuclear Zn{sup II} active site. Therefore, a histidine residue can function as the general acid/base in hydrolysis reactions of peptides and, through analogy of

  14. Three-dimensional transesophageal echocardiography for descending aortic atheroma: a preliminary study.

    Hammoudi, Nadjib; Ihaddaden, Malek; Lang, Sylvie; Laveau, Florent; Ederhy, Stephane; Michel, Pierre-Louis; Alamowitch, Sonia; Cohen, Ariel

    2014-12-01

    Transesophageal echocardiography (TEE) is an efficient method for characterization of aortic atherosclerotic plaques (AAP). The aim of our study was to evaluate the feasibility and the additional contribution of three-dimensional (3D) TEE in the evaluation of AAPs in descending thoracic aorta. We studied 82 patients referred for TEE regardless of the indication. All patients underwent two-dimensional (2D) conventional acquisitions. A 3D TEE study was performed for all AAPs localized in the descending thoracic aorta. Thickness, degree of calcification, the presence of ulceration or mobile debris were compared for 2D and 3D modes. From 3D data, three types of AAPs were defined according to their morphological characteristics (surface and contours). Among 192 AAPs found on 2D acquisition, 189 (98.4 %) were also identified by 3D TEE. For AAP characterization, agreement was good between 2D TEE and 2D extracted from 3D with the multiplanar reconstruction mode: 83.6 % (k = 0.69) for thickness and 82.5 % (k = 0.72) for degree of calcification. All AAPs ulcerations (n = 13) and mobile debris (n = 3) seen in 2D were identified in 3D. 2D characteristics of the 3D AAPs' morphological types were different: type I plaques were thin and rarely calcified; type III plaques were thicker and often calcified; and type II presented intermediate characteristics. There was overlap among groups and the 3D morphology could not be predicted from 2D data. 3D TEE is a feasible method for the analysis of AAPs. In addition to conventional characterization, 3D TEE provides a new morphological approach to AAPs. PMID:25056253

  15. Asparaginase-associated pancreatitis in children with acute lymphoblastic leukaemia in the NOPHO ALL2008 protocol.

    Raja, Raheel A; Schmiegelow, Kjeld; Albertsen, Birgitte K; Prunsild, Kaie; Zeller, Bernward; Vaitkeviciene, Goda; Abrahamsson, Jonas; Heyman, Mats; Taskinen, Mervi; Harila-Saari, Arja; Kanerva, Jukka; Frandsen, Thomas L

    2014-04-01

    L-asparaginase is an important drug in the treatment of childhood acute lymphoblastic leukaemia (ALL). Treatment is associated with several toxicities, including acute pancreatitis. Clinical course, presentation, re-exposure to L-asparginase after pancreatitis and risk of recurrent pancreatitis within an asparaginase-intensive protocol has been poorly reported. Children (1-17 years) on the ongoing Nordic Society of Paediatric Haematology and Oncology (NOPHO) ALL2008 protocol with asparaginase-associated pancreatitis (AAP) diagnosed between 2008 and 2012 were identified through the online NOPHO ALL toxicity registry. NOPHO ALL2008 includes eight or 15 doses of intramuscular pegylated L-asparginase (PEG-asparaginase) 1000 iu/m(2) /dose at 2-6 weeks intervals, with a total of 30 weeks of exposure to PEG-asparaginase (clinicaltrials.gov no: NCT00819351). Of 786 children, 45 were diagnosed with AAP with a cumulative risk of AAP of 5·9%. AAP occurred after a median of five doses (range 1-13), and 11 d (median) from the latest administration of PEG-Asparaginase. Thirteen patients developed pseudocysts (30%) and 11 patients developed necrosis (25%). One patient died from pancreatitis. Twelve AAP patients were re-exposed to L-asparginase, two of whom developed mild AAP once more, after four and six doses respectively. In conclusion, re-exposure to PEG-asparaginase in ALL patients with mild AAP seems safe. PMID:24428625

  16. Monoclonal antibodies against accumulation-associated protein affect EPS biosynthesis and enhance bacterial accumulation of Staphylococcus epidermidis.

    Jian Hu

    Full Text Available Because there is no effective antibiotic to eradicate Staphylococcus epidermidis biofilm infections that lead to the failure of medical device implantations, the development of anti-biofilm vaccines is necessary. Biofilm formation by S. epidermidis requires accumulation-associated protein (Aap that contains sequence repeats known as G5 domains, which are responsible for the Zn(2+-dependent dimerization of Aap to mediate intercellular adhesion. Antibodies against Aap have been reported to inhibit biofilm accumulation. In the present study, three monoclonal antibodies (MAbs against the Aap C-terminal single B-repeat construct followed by the 79-aa half repeat (AapBrpt1.5 were generated. MAb(18B6 inhibited biofilm formation by S. epidermidis RP62A to 60% of the maximum, while MAb(25C11 and MAb(20B9 enhanced biofilm accumulation. All three MAbs aggregated the planktonic bacteria to form visible cell clusters. Epitope mapping revealed that the epitope of MAb(18B6, which recognizes an identical area within AapBrpt constructs from S. epidermidis RP62A, was not shared by MAb(25C11 and MAb(20B9. Furthermore, all three MAbs were found to affect both Aap expression and extracellular polymeric substance (EPS, including extracellular DNA and PIA biosynthesis in S. epidermidis and enhance the cell accumulation. These findings contribute to a better understanding of staphylococcal biofilm formation and will help to develop epitope-peptide vaccines against staphylococcal infections.

  17. Patterns in Abundance, Cell Size and Pigment Content of Aerobic Anoxygenic Phototrophic Bacteria along Environmental Gradients in Northern Lakes.

    Lisa Fauteux

    Full Text Available There is now evidence that aerobic anoxygenic phototrophic (AAP bacteria are widespread across aquatic systems, yet the factors that determine their abundance and activity are still not well understood, particularly in freshwaters. Here we describe the patterns in AAP abundance, cell size and pigment content across wide environmental gradients in 43 temperate and boreal lakes of Québec. AAP bacterial abundance varied from 1.51 to 5.49 x 105 cells mL-1, representing <1 to 37% of total bacterial abundance. AAP bacteria were present year-round, including the ice-cover period, but their abundance relative to total bacterial abundance was significantly lower in winter than in summer (2.6% and 7.7%, respectively. AAP bacterial cells were on average two-fold larger than the average bacterial cell size, thus AAP cells made a greater relative contribution to biomass than to abundance. Bacteriochlorophyll a (BChla concentration varied widely across lakes, and was not related to AAP bacterial abundance, suggesting a large intrinsic variability in the cellular pigment content. Absolute and relative AAP bacterial abundance increased with dissolved organic carbon (DOC, whereas cell-specific BChla content was negatively related to chlorophyll a (Chla. As a result, both the contribution of AAP bacteria to total prokaryotic abundance, and the cell-specific BChla pigment content were positively correlated with the DOC:Chla ratio, both peaking in highly colored, low-chlorophyll lakes. Our results suggest that photoheterotrophy might represent a significant ecological advantage in highly colored, low-chlorophyll lakes, where DOC pool is chemically and structurally more complex.

  18. Alarm Reduction Processing of Advanced Nuclear Power Plant Using Data Mining and Active Database Technologies

    The purpose of the Advanced Alarm Processing (AAP) is to extract only the most important and the most relevant data out of large amount of available information. It should be noted that the integrity of the knowledge base is the most critical in developing a reliable AAP. This paper proposes a new approach to an AAP by using Event-Condition-Action(ECA) rules that can be automatically triggered by an active database. Also this paper proposed a knowledge acquisition method using data mining techniques to obtain the integrity of the alarm knowledge

  19. Cow's milk - infants

    ... page: //medlineplus.gov/ency/article/002448.htm Cow's milk - infants To use the sharing features on this ... old, you should not feed your baby cow's milk, according to the American Academy of Pediatrics (AAP). ...

  20. Blonanserin Augmentation of Atypical Antipsychotics in Patients with Schizophrenia-Who Benefits from Blonanserin Augmentation?: An Open-Label, Prospective, Multicenter Study

    Woo, Young Sup; Park, Joo Eon; Kim, Do-Hoon; Sohn, Inki; Hwang, Tae-Yeon; Park, Young-Min; Jon, Duk-In; Jeong, Jong-Hyun

    2016-01-01

    Objective The purpose of this study was to investigate the efficacy and tolerability of atypical antipsychotics (AAPs) with augmentation by blonanserin in schizophrenic patients. Methods aA total of 100 patients with schizophrenia who were partially or completely unresponsive to treatment with an AAP were recruited in this 12-week, open-label, non-comparative, multicenter study. Blonanserin was added to their existing AAP regimen, which was maintained during the study period. Efficacy was primarily evaluated using the Positive and Negative Syndrome Scale (PANSS) at baseline and at weeks 2, 4, 8, and 12. Predictors for PANSS response (≥20% reduction) were investigated. Results The PANSS total score was significantly decreased at 12 weeks of blonanserin augmentation (-21.0±18.1, F=105.849, pschizophrenia who were partially or completely unresponsive to treatment with an AAP.

  1. Swearing

    ... Find a Pediatrician Newsletters Symptom Checker Apps E-Magazine Our Mission Our Mission Our Mission AAP in ... swearing may be just another symptom of a psychological or social disturbance. In this situation, talk to ...

  2. Signs of Overload

    ... Find a Pediatrician Newsletters Symptom Checker Apps E-Magazine Our Mission Our Mission Our Mission AAP in ... that it is undermining your child's physical or psychological well-being. Here are some clues that stress ...

  3. Masturbation

    ... Find a Pediatrician Newsletters Symptom Checker Apps E-Magazine Our Mission Our Mission Our Mission AAP in ... or public masturbation may indicate a more serious psychological or personal problem. It could be a sign ...

  4. Mental Health Care: Who's Who

    ... Find a Pediatrician Newsletters Symptom Checker Apps E-Magazine Our Mission Our Mission Our Mission AAP in ... used to measure a young person’s intellect and psychological health. All of the mental health counselors listed ...

  5. Rapid determination of total phenols in seawater by 4-aminoantipyrine colorimetry

    Kadam, A.N.; Bhangale, V.P.

    A rapid and efficient 4-aminoantipyrine (4-AAP) colorimetric method without any cleanup step to determine total phenols in seawater is described. Efficiency of the method for seawater using external addition of phenol concentrations with working...

  6. Spontaneous Ejaculations in an Adolescent With Olanzapine Use: Case Report.

    Yektaş, Çiğdem; Tufan, Ali Evren

    2016-01-01

    Olanzapine (OLZ) is a second-generation atypical antipsychotic (AAP) frequently used in acute and maintenance treatment of children and adolescents with psychotic disorders. Most commonly reported sexual problems caused by AAPs are decreases in libido or arousal, erectile dysfunctions, reduced/abnormal ejaculations, and problems in achieving or maintaining orgasm. Olanzapine is less commonly implicated for those adverse effects, which may reflect its more selective affinity to dopaminergic receptors or more transient effects on prolactin levels. Sexual dysfunction with AAPs, including OLZ, is reported predominantly in adult patients and adverse sexual effects in prepubertal/peripubertal patients with psychosis received scant attention. Studies and reports of sexual adverse effects of AAPs in this special population may benefit clinicians. Therefore, in this study, we report an adolescent male patient with psychosis who developed spontaneous ejaculations with OLZ and whose complaints remitted with change in treatment. PMID:26882319

  7. Circumcision

    Circumcision is a surgical procedure to remove the foreskin, the skin that covers the tip of the ... AAP), there are medical benefits and risks to circumcision. Possible benefits include a lower risk of urinary ...

  8. American Academy of Pediatrics

    ... of death. Study Urges Child Development Screening for Refugee Families Refugee children’s development is at risk due to the ... one-hour webinar. Health Topics Healthy Foster Care America AAP and its partners strive to improve the ...

  9. Positive recurrence of processes associated to crystal growth models.

    Andjel, A.; Menshikov, M. V.; Sisko, V.

    2006-01-01

    Comment: Published at http://dx.doi.org/10.1214/105051606000000079 in the Annals of Applied Probability (http://www.imstat.org/aap/) by the Institute of Mathematical Statistics (http://www.imstat.org)

  10. Normal Functioning Family

    ... Spread the Word Shop AAP Find a Pediatrician Family Life Medical Home Family Dynamics Adoption & Foster Care ... Español Text Size Email Print Share Normal Functioning Family Page Content Article Body Is there any way ...

  11. Family Disruptions

    ... Spread the Word Shop AAP Find a Pediatrician Family Life Medical Home Family Dynamics Adoption & Foster Care ... Life Listen Español Text Size Email Print Share Family Disruptions Page Content Article Body No matter how ...

  12. Roles within the Family

    ... Spread the Word Shop AAP Find a Pediatrician Family Life Medical Home Family Dynamics Adoption & Foster Care ... Text Size Email Print Share Roles Within the Family Page Content Article Body Families are not democracies. ...

  13. Family Arguments

    ... Spread the Word Shop AAP Find a Pediatrician Family Life Medical Home Family Dynamics Adoption & Foster Care ... Life Listen Español Text Size Email Print Share Family Arguments Page Content Article Body We seem to ...

  14. Hib Photos

    ... St. Paul, Minnesota Cerebrospinal fluid culture positive for Haemophilus influenzae , type b (Gram stain) www.vaccineinformation.org/photos/hib_aap001.jpg Copyright American Academy of Pediatrics Haemophilus influenzae type b. Cellulitis of the arm proven by blood culture ...

  15. Swimming Pool Safety

    ... Spread the Word Shop AAP Find a Pediatrician Safety & Prevention Immunizations All Around At Home At Play ... Español Text Size Email Print Share Swimming Pool Safety Page Content ​What is the best way to ...

  16. Feeding Vegetarian and Vegan Infants and Toddlers

    ... Men For Women For Seniors Feeding Vegetarian and Vegan Infants and Toddlers By Dayle Hayes, MS, RD ... of Pediatrics (AAP) agree: Well-planned vegetarian and vegan eating patterns are healthy for infants and toddlers. ...

  17. Tonsillitis

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a ... Print Share Tonsillitis Page Content Article Body My child has tonsillitis. Will he need to have his ...

  18. Competition and Cheating

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a ... value in these older peer relationships. If Your Child Cheats If you are confronted with a situation ...

  19. Movement and Coordination

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a Pediatrician ... Movement and Coordination Page Content Article Body At this age, your child will seem to be continually on the go— ...

  20. Drinks to Prevent Dehydration in a Vomiting Child

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a ... Share Drinks to Prevent Dehydration in a Vomiting Child Page Content Article Body For vomiting children, the ...

  1. Safety for Your Child: 6 Years

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a Pediatrician ... > Safety for Your Child: 6 Years Ages & Stages Listen Español Text Size ...

  2. Croup and Your Young Child

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a ... Size Email Print Share Croup and Your Young Child Page Content Article Body Croup is a co​ ...

  3. Chronic Nosebleeds: What to Do

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a ... What To Do Page Content Article Body My child gets a lot of nosebleeds. What should we ...

  4. Wings and Stings

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a ... See “Stinging Insects and Asthma” below.) Is Your Child at Risk? How do you know if you ...

  5. Friend or Foe?

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a Pediatrician ... Friend or Foe? Page Content Article Body Help your child navigate his social world by equipping him with ...

  6. Asthma Triggers and What to Do about Them

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a ... for exposure to these same things. Help Your Child Avoid Triggers While it is impossible to make ...

  7. Immunizations: Active vs. Passive

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP ... Active vs. Passive Page Content Article Body Pediatricians can protect your child by administering not only active immunizations , but sometimes ...

  8. Car Sickness

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a ... Share Car Sickness Page Content Article Body My child gets sick in the car quite often. How ...

  9. Preventing the Spread of Illness in Child Care or School

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a ... Print Share Preventing the Spread of Illness in Child Care or School Page Content Article Body In ...

  10. Treating Vomiting

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a ... vomits these fluids, notify your pediatrician. Modify Your Child's Diet For the first twenty-four hours or ...

  11. Child Care Provider's Guide to Safe Sleep

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a Pediatrician ... Children > Family Life > Work & Play > A Child Care Provider's Guide to Safe Sleep Family Life ...

  12. Eczema

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a ... Email Print Share Eczema: How to Help Your Child Avoid the Itch Page Content Article Body Eczema ...

  13. Iron Supplements Reduce Behavior Problems in Low Birth Weight Infants

    ... Content Social Media Toolkit Donate Now Corporate Relationships Advertise with AAP Help/Feedback Recent a a a print email share Facebook Twitter Iron Supplements Reduce Behavior Problems in Low ...

  14. Baltimore Eesti Selts - 75 / Vilve Ladon ; fotod: Fred Ise

    Ladon, Vilve

    2010-01-01

    Baltimore Eesti Majas tähistati 2. okt. 2010 Baltimore Eesti Seltsi aastapäeva. Eesti suursaatkonna esimene sekretär poliitilistes küsimustes Aap Neljas edastas president Toomas Hendrik Ilvese tervituse

  15. Social Development:: 2 Year Olds

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a Pediatrician ... Ages & Stages > Toddler > Social Development: 2 Year Olds Ages & Stages Listen Español Text ...

  16. Concerns Girls Have about Puberty

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a Pediatrician ... Content Article Body Girls have pubertal concerns and worries, too, including: Breast Development Some girls also have anxieties about breast development. ...

  17. Emotional Development: 2 Year Olds

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a Pediatrician ... & Stages > Toddler > Emotional Development: 2 Year Olds Ages & Stages Listen Español Text ...

  18. Cognitive Development: Two-Year-Old

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a Pediatrician ... & Stages > Toddler > Cognitive Development: Two-Year-Old Ages & Stages Listen Español Text ...

  19. Cognitive Development: One-Year-Old

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a Pediatrician ... & Stages > Toddler > Cognitive Development: One-Year-Old Ages & Stages Listen Español Text ...

  20. Normal Child Behavior

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a ... is "normal" depends upon the child's level of development, which can vary greatly among children of the ...

  1. Eyelid Problems

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a ... irregular shape (astigmatism), it will threaten normal vision development and must be corrected as early as possible. ...

  2. Emotional Development: 1 Year Olds

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a Pediatrician ... & Stages > Toddler > Emotional Development: 1 Year Olds Ages & Stages Listen Español Text ...

  3. Social Development: 1 Year Olds

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a Pediatrician ... Ages & Stages > Toddler > Social Development: 1 Year Olds Ages & Stages Listen Español Text ...

  4. Physical Development: What's Normal? What's Not?

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a Pediatrician ... > Puberty > Physical Development: What’s Normal? What’s Not? Ages & Stages Listen Español ...

  5. AcEST: DK953689 [AcEST

    Full Text Available TST39A01NGRL0018_H03 613 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0018_H03. 5' end seq ... e-32 tr|Q2AAC8|Q2AAC8_9ASTR Cysteine proteinase OS=Platycodon ... grandif... 142 1e-32 tr|A3AAP5|A3AAP5_ORYSJ Putati ...

  6. Management of acute gastroenteritis in healthy children in Lebanon - A national survey

    Nahida Rifai; Sawsan Mourad; Aouni Alameddine

    2010-01-01

    Background: Acute gastroenteritis remains a common condition among infants and children throughout the world. In 1996, The American Academy of Pediatrics (AAP) revised its recommendations for the treatment of infants and children with acute gastroenteritis. Aim: The purpose of this survey was to determine how closely current treatment among Lebanese pediatricians compares with the AAP recommendations and to determine the impact of such management on the healthcare system. Patients and Methods...

  7. Diet quality, nutrient intake, weight status, and feeding environments of girls meeting or exceeding the American Academy of Pediatrics recommendations for total dietary fat

    Lee, Y.; Birch, L. L.

    2002-01-01

    The American Academy of Pediatrics (AAP) recommends that children consume no more than 30% but no less than 20% of energy as dietary fat intake, and tills recommendation, is accompanied by suggestions that fat calories should be replaced by eating more grain products, fruits, vegetables, low fat datiy products, beans, lean meat, poultry, fish, and other protein rich foods. In comparing diets of girls meeting this AAP recommendation with girls who consumed diets higher in fat, we noted that gi...

  8. Gene : CBRC-OCUN-01-0570 [SEVENS

    Full Text Available asal 1 receptor 2 [Homo sapiens] sp|Q8NFZ6|VN1R2_HUMAN Vomeronasal type-1 receptor 2 (V1r-like receptor 2) (...hGPCR25) dbj|BAB89320.1| putative G-protein coupled receptor [Homo sapiens] gb|AAP85616.1| pheromone receptor [Homo sapie...ns] gb|AAP85617.1| pheromone receptor [Homo sapiens] 1e-11 34% gnl|U...VISISTTCLLSVFQAITISPMNSRWAESKPKLQRLLAFPVTKTDTAIGILSTCHDVLWFLLMLWASGSTVFILHCTGRGSNTFIRATSCQDAARDHSSPKNPIEHLGLSLLLAFQLPVPLFLSHKCCVCTGRKRQFSQFRRI ...

  9. Catalytically distinct states captured in a crystal lattice: the substrate-bound and scavenger states of acylaminoacyl peptidase and their implications for functionality.

    Menyhárd, Dóra Karancsiné; Orgován, Zoltán; Szeltner, Zoltán; Szamosi, Ilona; Harmat, Veronika

    2015-03-01

    Acylaminoacyl peptidase (AAP) is an oligopeptidase that only cleaves short peptides or protein segments. In the case of AAP from Aeropyrum pernix (ApAAP), previous studies have led to a model in which the clamshell-like opening and closing of the enzyme provides the means of substrate-size selection. The closed form of the enzyme is catalytically active, while opening deactivates the catalytic triad. The crystallographic results presented here show that the open form of ApAAP is indeed functionally disabled. The obtained crystal structures also reveal that the closed form is penetrable to small ligands: inhibitor added to the pre-formed crystal was able to reach the active site of the rigidified protein, which is only possible through the narrow channel of the propeller domain. Molecular-dynamics simulations investigating the structure of the complexes formed with longer peptide substrates showed that their binding within the large crevice of the closed form of ApAAP leaves the enzyme structure unperturbed; however, their accessing the binding site seems more probable when assisted by opening of the enzyme. Thus, the open form of ApAAP corresponds to a scavenger of possible substrates, the actual cleavage of which only takes place if the enzyme is able to re-close. PMID:25760596

  10. Ascorbic acid prevents acetaminophen-induced hepatotoxicity in mice by ameliorating glutathione recovery and autophagy.

    Kurahashi, Toshihiro; Lee, Jaeyong; Nabeshima, Atsunori; Homma, Takujiro; Kang, Eun Sil; Saito, Yuka; Yamada, Sohsuke; Nakayama, Toshiyuki; Yamada, Ken-Ichi; Miyata, Satoshi; Fujii, Junichi

    2016-08-15

    Aldehyde reductase (AKR1A) plays a role in the biosynthesis of ascorbic acid (AsA), and AKR1A-deficient mice produce about 10-15% of the AsA that is produced by wild-type mice. We found that acetaminophen (AAP) hepatotoxicity was aggravated in AKR1A-deficient mice. The pre-administration of AsA in the drinking water markedly ameliorated the AAP hepatotoxicity in the AKR1A-deficient mice. Treatment of the mice with AAP decreased both glutathione and AsA levels in the liver in the early phase after AAP administration, and an AsA deficiency delayed the recovery of the glutathione content in the healing phase. While in cysteine supply systems; a neutral amino acid transporter ASCT1, a cystine transporter xCT, enzymes for the transsulfuration pathway, and autophagy markers, were all elevated in the liver as the result of the AAP treatment, the AsA deficiency suppressed their induction. Thus, AsA appeared to exert a protective effect against AAP hepatotoxicity by ameliorating the supply of cysteine that is available for glutathione synthesis as a whole. Because some drugs produce reactive oxygen species, resulting in the consumption of glutathione during the metabolic process, the intake of sufficient amounts of AsA would be beneficial for protecting against the hepatic damage caused by such drugs. PMID:27288086

  11. CT and MR imaging in patients with localized acute abdominal pain

    Full text: Acute abdominal pain (AAP) is one of the most common causes for admissions to emergency departments. Clinical presentation, physical and laboratory examinations are often inconclusive and, therefore, imaging evaluation is required. Ultrasonography is efficient in the evaluation of gallbladder and gynecologic conditions and it is considered the first-line examination in many centres. Plain radiography and barium studies are often falsely normal or non-specific, especially in the most common conditions related with rightsided AAP and their use has significantly decreased. CT is a rapid and accurate imaging modality in the diagnosis of common (appendicitis, acute cholecystitis, biliary obstruction, duodenal ulcer perforation) or uncommon (mesenteric adenitis, liver abscess, cholangitis etc) conditions associated with right-sided AAP. CT may demonstrate a number of either specific or sensitive imaging findings for the confident diagnosis of diseases manifested with AAP. Moreover, CT has the advantage to suggest alternative diagnoses, if the suspected clinical diagnosis is unconfirmed. Different examination protocols and different strategies for the application of oral-rectal-intravenous contrast agents have been proposed to balance diagnostic accuracy to time effectiveness and radiation dose. MRI is suggested for AAP related to biliary abnormalities or to gynecologic diseases and it may be used as a complementary examination in pregnant patients. Imaging evaluation is an indispensable part in the diagnostic work up of most patients with AAP. CT has gained widespread acceptance, as offering more accurate and confident diagnosis and its use has changed management of the patients in 28-60% of the cases

  12. Involvement of mitochondria in acetaminophen-induced apoptosis and hepatic injury Roles of cytochrome c, Bax, Bid, and caspases

    The role of apoptosis in acetaminophen (AAP)-induced hepatic injury was investigated. Six hours after AAP administration to BALB/c mice, a significant loss of hepatic mitochondrial cytochrome c was observed that was similar in extent to the loss observed after in vivo activation of CD95 by antibody treatment. AAP-induced loss of mitochondrial cytochrome c coincided with the appearance in the cytosol of a fragment corresponding to truncated Bid (tBid). At the same time, tBid became detectable in the mitochondrial fraction, and concomitantly, Bax was found translocated to mitochondria. However, AAP failed to activate the execution caspases 3 and 7 as evidenced by a lack of procaspase processing and the absence of an increase in caspase-3-like activity. In contrast, the administration of the pan-inhibitor of caspases, benzyloxycarbonyl-Val-Ala-DL-Asp-fluoromethylketone (but not its analogue benzyloxycarbonyl-Phe-Ala-fluoromethylketone) prevented the development of liver injury by AAP and the appearance of apoptotic parenchymal cells. This correlated with the inhibition of the processing of Bid to tBid. The caspase inhibitor failed to prevent both the redistribution of Bax to the mitochondria and the loss of cytochrome c. In conclusion, apoptosis is an important causal event in the initiation of the hepatic injury inflicted by AAP. However, as suggested by the lack of activation of the main execution caspases, apoptosis is not properly executed and degenerates into necrosis

  13. Korean Medication Algorithm Project for Bipolar Disorder: third revision

    Woo YS

    2015-02-01

    Full Text Available Young Sup Woo,1 Jung Goo Lee,2,3 Jong-Hyun Jeong,1 Moon-Doo Kim,4 Inki Sohn,5 Se-Hoon Shim,6 Duk-In Jon,7 Jeong Seok Seo,8 Young-Chul Shin,9 Kyung Joon Min,10 Bo-Hyun Yoon,11 Won-Myong Bahk1 1Department of Psychiatry, College of Medicine, The Catholic University of Korea, Seoul, South Korea; 2Department of Psychiatry, Inje University Haeundae Paik Hospital, Busan, South Korea;3Paik Institute for Clinical Research, Inje Univeristy, Busan, South Korea; 4Department of Psychiatry, Jeju National University Hospital, Jeju, South Korea; 5Department of Psychiatry, Keyo Hospital, Keyo Medical Foundation, Uiwang, South Korea; 6Department of Psychiatry, Soonchunhyang University Cheonan Hospital, Soonchunhyang University, Cheonan, South Korea; 7Department of Psychiatry, Sacred Heart Hospital, Hallym University, Anyang, South Korea; 8Department of Psychiatry, School of Medicine, Konkuk University, Chungju, South Korea; 9Department of Psychiatry, Kangbuk Samsung Hospital, School of Medicine, Sungkyunkwan University, Seoul, South Korea; 10Department of Psychiatry, College of Medicine, Chung-Ang University, Seoul, South Korea; 11Department of Psychiatry, Naju National Hospital, Naju, South Korea Objective: To constitute the third revision of the guidelines for the treatment of bipolar disorder issued by the Korean Medication Algorithm Project for Bipolar Disorder (KMAP-BP 2014. Methods: A 56-item questionnaire was used to obtain the consensus of experts regarding pharmacological treatment strategies for the various phases of bipolar disorder and for special populations. The review committee included 110 Korean psychiatrists and 38 experts for child and adolescent psychiatry. Of the committee members, 64 general psychiatrists and 23 child and adolescent psychiatrists responded to the survey. Results: The treatment of choice (TOC for euphoric, mixed, and psychotic mania was the combination of a mood stabilizer (MS and an atypical antipsychotic (AAP; the TOC for

  14. Transformation of acetaminophen during water chlorination treatment: kinetics and transformation products identification.

    Cao, Fei; Zhang, Mengtao; Yuan, Shoujun; Feng, Jingwei; Wang, Qiquan; Wang, Wei; Hu, Zhenhu

    2016-06-01

    As a high-consumption drug in the world, acetaminophen (AAP) has been widely detected in natural waters and wastewaters. Its reactivity and the transformation products formed during chlorination may greatly threaten the safety of drinking water. The reaction kinetics of AAP during chlorination was investigated in this study. The results showed that the reaction kinetics could be well described with a kinetics model of -d[AAP]/dt = k app[AAP]t (0.63)[Cl2]t (1.37). The values of apparent rate constant (k app) were dependent on reaction temperature, ammonium, and pH. With the increase in reaction temperature from 5.0 ± 1.0 to 40.0 ± 1.0 °C, the removal efficiency of AAP increased from 60 to 100 %. When ammonium was present in the solution at 2.0 mg/L, the transformation of AAP was inhibited due to the rapid formation of chloramines. The maximum of k app was 0.58 × 10(2) M(-1) · min(-1) at pH 9.0, and the minimum was 0.27 M(-1) · min(-1) at pH 11.0. A low mineralization of AAP (about 7.2 %) with chlorination was observed through TOC analysis, implying the formation of plenty of transformation products during chlorination. The main transformation products, hydroquinone and two kinds of chlorinated compounds, monochlorinated acetaminophen and dichlorinated acetaminophen, were detected in gas chromatography-mass spectrometry analysis. PMID:26983813

  15. Diversity of cultivated and metabolically active aerobic anoxygenic phototrophic bacteria along an oligotrophic gradient in the Mediterranean Sea

    Jeanthon, C.; Boeuf, D.; Dahan, O.; Le Gall, F.; Garczarek, L.; Bendif, E. M.; Lehours, A.-C.

    2011-07-01

    Aerobic anoxygenic phototrophic (AAP) bacteria play significant roles in the bacterioplankton productivity and biogeochemical cycles of the surface ocean. In this study, we applied both cultivation and mRNA-based molecular methods to explore the diversity of AAP bacteria along an oligotrophic gradient in the Mediterranean Sea in early summer 2008. Colony-forming units obtained on three different agar media were screened for the production of bacteriochlorophyll-a (BChl-a), the light-harvesting pigment of AAP bacteria. BChl-a-containing colonies represented a low part of the cultivable fraction. In total, 54 AAP strains were isolated and the phylogenetic analyses based on their 16S rRNA and pufM genes showed that they were all affiliated to the Alphaproteobacteria. The most frequently isolated strains belonged to Citromicrobium bathyomarinum, and Erythrobacter and Roseovarius species. Most other isolates were related to species not reported to produce BChl-a and/or may represent novel taxa. Direct extraction of RNA from seawater samples enabled the analysis of the expression of pufM, the gene coding for the M subunit of the reaction centre complex of aerobic anoxygenic photosynthesis. Clone libraries of pufM gene transcripts revealed that most phylotypes were highly similar to sequences previously recovered from the Mediterranean Sea and a large majority (~94 %) was affiliated to the Gammaproteobacteria. The most abundantly detected phylotypes occurred in the western and eastern Mediterranean basins. However, some were exclusively detected in the eastern basin, reflecting the highest diversity of pufM transcripts observed in this ultra-oligotrophic region. To our knowledge, this is the first study to document extensively the diversity of AAP isolates and to unveil the active AAP community in an oligotrophic marine environment. By pointing out the discrepancies between culture-based and molecular methods, this study highlights the existing gaps in the understanding

  16. Picoplankton Bloom in Global South? A High Fraction of Aerobic Anoxygenic Phototrophic Bacteria in Metagenomes from a Coastal Bay (Arraial do Cabo--Brazil).

    Cuadrat, Rafael R C; Ferrera, Isabel; Grossart, Hans-Peter; Dávila, Alberto M R

    2016-02-01

    Marine habitats harbor a great diversity of microorganism from the three domains of life, only a small fraction of which can be cultivated. Metagenomic approaches are increasingly popular for addressing microbial diversity without culture, serving as sensitive and relatively unbiased methods for identifying and cataloging the diversity of nucleic acid sequences derived from organisms in environmental samples. Aerobic anoxygenic phototrophic bacteria (AAP) play important roles in carbon and energy cycling in aquatic systems. In oceans, those bacteria are widely distributed; however, their abundance and importance are still poorly understood. The aim of this study was to estimate abundance and diversity of AAPs in metagenomes from an upwelling affected coastal bay in Arraial do Cabo, Brazil, using in silico screening for the anoxygenic photosynthesis core genes. Metagenomes from the Global Ocean Sample Expedition (GOS) were screened for comparative purposes. AAPs were highly abundant in the free-living bacterial fraction from Arraial do Cabo: 23.88% of total bacterial cells, compared with 15% in the GOS dataset. Of the ten most AAP abundant samples from GOS, eight were collected close to the Equator where solar irradiation is high year-round. We were able to assign most retrieved sequences to phylo-groups, with a particularly high abundance of Roseobacter in Arraial do Cabo samples. The high abundance of AAP in this tropical bay may be related to the upwelling phenomenon and subsequent picoplankton bloom. These results suggest a link between upwelling and light abundance and demonstrate AAP even in oligotrophic tropical and subtropical environments. Longitudinal studies in the Arraial do Cabo region are warranted to understand the dynamics of AAP at different locations and seasons, and the ecological role of these unique bacteria for biogeochemical and energy cycling in the ocean. PMID:26871866

  17. Advanced Avionics and Processor Systems for a Flexible Space Exploration Architecture

    Keys, Andrew S.; Adams, James H.; Smith, Leigh M.; Johnson, Michael A.; Cressler, John D.

    2010-01-01

    The Advanced Avionics and Processor Systems (AAPS) project, formerly known as the Radiation Hardened Electronics for Space Environments (RHESE) project, endeavors to develop advanced avionic and processor technologies anticipated to be used by NASA s currently evolving space exploration architectures. The AAPS project is a part of the Exploration Technology Development Program, which funds an entire suite of technologies that are aimed at enabling NASA s ability to explore beyond low earth orbit. NASA s Marshall Space Flight Center (MSFC) manages the AAPS project. AAPS uses a broad-scoped approach to developing avionic and processor systems. Investment areas include advanced electronic designs and technologies capable of providing environmental hardness, reconfigurable computing techniques, software tools for radiation effects assessment, and radiation environment modeling tools. Near-term emphasis within the multiple AAPS tasks focuses on developing prototype components using semiconductor processes and materials (such as Silicon-Germanium (SiGe)) to enhance a device s tolerance to radiation events and low temperature environments. As the SiGe technology will culminate in a delivered prototype this fiscal year, the project emphasis shifts its focus to developing low-power, high efficiency total processor hardening techniques. In addition to processor development, the project endeavors to demonstrate techniques applicable to reconfigurable computing and partially reconfigurable Field Programmable Gate Arrays (FPGAs). This capability enables avionic architectures the ability to develop FPGA-based, radiation tolerant processor boards that can serve in multiple physical locations throughout the spacecraft and perform multiple functions during the course of the mission. The individual tasks that comprise AAPS are diverse, yet united in the common endeavor to develop electronics capable of operating within the harsh environment of space. Specifically, the AAPS tasks for

  18. Ultrasonic degradation of acetaminophen and naproxen in the presence of single-walled carbon nanotubes

    Im, Jong-Kwon; Heo, Jiyong; Boateng, Linkel K. [Department of Civil and Environmental Engineering, University of South Carolina, Columbia, SC 29208 (United States); Her, Namguk [Department of Chemistry and Environmental Sciences, Korea Army Academy at Young-Cheon, 135-1, Changhari, Kokyungmeon, Young-cheon, Gyeongbuk 770-849 (Korea, Republic of); Flora, Joseph R.V. [Department of Civil and Environmental Engineering, University of South Carolina, Columbia, SC 29208 (United States); Yoon, Jaekyung [Korea Institute of Energy Research, New and Renewable Energy Research Division, 71-2 Jang-Dong, Yuseong-Gu, Daejeon 305-343 (Korea, Republic of); Zoh, Kyung-Duk [Department of Environmental Health, School of Public Health, Seoul National University, Seoul 151-742 (Korea, Republic of); Yoon, Yeomin, E-mail: yoony@cec.sc.edu [Department of Civil and Environmental Engineering, University of South Carolina, Columbia, SC 29208 (United States)

    2013-06-15

    Highlights: • Sonodegradation of acetaminophen and naproxen was performed. • Degradation was enhanced with the dispersion of SWNTs under US irradiation. • Synergistic effect on the degradation PhACs was observed in US/SWNT process. • Significant removal of dissolved organic carbon was achieved. -- Abstract: Ultrasonic (US) and single-walled carbon nanotube (SWNT)-catalyzed ultrasonic (US/SWNT) degradation of a pharmaceutical (PhAC) mixture of acetaminophen (AAP) and naproxen (NPX) used as analgesics was carried out in water. In the absence of SWNTs, maximum degradations of AAP and NPX occurred at a high frequency (1000 kHz) and under acidic conditions (pH 3) and different solution temperatures (25 °C at 28 kHz and 35 °C at 1000 kHz) during US reactions. Rapid degradation of PhACs occurred within 10 min at 28 kHz (44.5% for AAP; 90.3% for NPX) and 1000 kHz (39.2% for AAP; 74.8% for NPX) at a SWNT concentration of 45 mg L{sup −1} under US/SWNT process, compared with 28 kHz (5.2% for AAP; 10.6% for NPX) and 1000 kHz (29.1% for AAP; 46.2% for NPX) under US process. Degradation was associated with the dispersion of SWNTs; small particles acted as nuclei during US reactions, enhancing the H{sub 2}O{sub 2} production yield. NPX removal was greater than AAP removal under all US-induced reaction and SWNT adsorption conditions, which is governed by the chemical properties of PhACs. Based on the results, the optimal treatment performance was observed at 28 kHz with 45 mg L{sup −1} SWNTs (US/SWNT) within 10 min.

  19. Ultrasonic degradation of acetaminophen and naproxen in the presence of single-walled carbon nanotubes

    Highlights: • Sonodegradation of acetaminophen and naproxen was performed. • Degradation was enhanced with the dispersion of SWNTs under US irradiation. • Synergistic effect on the degradation PhACs was observed in US/SWNT process. • Significant removal of dissolved organic carbon was achieved. -- Abstract: Ultrasonic (US) and single-walled carbon nanotube (SWNT)-catalyzed ultrasonic (US/SWNT) degradation of a pharmaceutical (PhAC) mixture of acetaminophen (AAP) and naproxen (NPX) used as analgesics was carried out in water. In the absence of SWNTs, maximum degradations of AAP and NPX occurred at a high frequency (1000 kHz) and under acidic conditions (pH 3) and different solution temperatures (25 °C at 28 kHz and 35 °C at 1000 kHz) during US reactions. Rapid degradation of PhACs occurred within 10 min at 28 kHz (44.5% for AAP; 90.3% for NPX) and 1000 kHz (39.2% for AAP; 74.8% for NPX) at a SWNT concentration of 45 mg L−1 under US/SWNT process, compared with 28 kHz (5.2% for AAP; 10.6% for NPX) and 1000 kHz (29.1% for AAP; 46.2% for NPX) under US process. Degradation was associated with the dispersion of SWNTs; small particles acted as nuclei during US reactions, enhancing the H2O2 production yield. NPX removal was greater than AAP removal under all US-induced reaction and SWNT adsorption conditions, which is governed by the chemical properties of PhACs. Based on the results, the optimal treatment performance was observed at 28 kHz with 45 mg L−1 SWNTs (US/SWNT) within 10 min

  20. Comparative validity of the Adult Attachment Interview and the Adult Attachment Projective.

    Jones-Mason, Karen; Allen, I Elaine; Hamilton, Steve; Weiss, Sandra J

    2015-01-01

    The Adult Attachment Interview (AAI) and the Adult Attachment Projective (AAP) purport to measure the same attachment classifications. The aims of this study were to determine the concurrent validity of the AAI and AAP, and to compare their ability to predict indicators of risk associated with socioeconomic status (SES), depression, and 5-HTTLPR genotype. Results indicated no agreement between AAI and AAP attachment classifications in a sample of late adolescents. As predicted, individuals classified as "unresolved" with regard to loss or trauma were significantly more likely to be of lower SES, have higher levels of depression, and have the 5-HTTLPR "ss" genotype than individuals with secure, preoccupied, or dismissing attachments. These associations, however, were only significant when attachment was classified with the AAI. Results suggest that the AAI and AAP measure different facets of attachment as a result of their unique methodologies and coding criteria. Further research is needed to support their comparability before investigators can assume that the AAP is a valid substitute for the AAI. PMID:26362584

  1. Influence of selected environmental factors on the abundance of aerobic anoxygenic phototrophs in peat-bog lakes.

    Lew, Sylwia; Lew, Marcin; Koblížek, Michal

    2016-07-01

    Aerobic anoxygenic phototrophs (AAPs) are photoheterotrophic prokaryotes that are widespread in many limnic and marine environments. So far, little is known about their distribution in peat-bog lakes. Seventeen peat-bog lakes were sampled during three summer seasons 2009, 2011, and 2012, and the vertical distribution of AAPs was determined by infrared epifluorescence microscopy. The analysis demonstrated that in the surface layers of the studied lakes, AAP abundance ranged from 0.3 to 12.04 × 10(5) cells mL(-1), which represents water column with minimum numbers in the anoxic bottom waters. We have shown that the AAP abundance was significantly positively correlated with the water pH, and the highest proportion of photoheterotrophs was found in peat-bog lakes with a pH between 6.7 and 7.6. Our results demonstrated an influence of water acidity on the abundance of AAPs, which may reflect a fundamental difference in the microbial composition between acidic and pH neutral peat-bog lakes. PMID:27032635

  2. Novel Spectrophotometric Method for Determination of Macro-paracetamol via Reaction with Bromine

    1999-01-01

    The reaction between Br2 and paracetamol(p-AAP) leads to the formation of a coloured product, which can be used for spectrophotometric determination of the p-AAP content in its pure form and in different pharmaceutical preparations with p-AAP. The stoichiometric composition of the reaction was found to be n(p-AAP)∶n(bromine)=1∶3. The effects of pH and time on the spectra of p-AAP-bromine redox reaction product were studied. The interference of different additives on the measured spectra of the obtained product was also studied. The results obtained by the present method were compared with those obtained by the standard method. The F- and t- test values were calculated for both of the applied procedures and they met a confidence level of 99%. The proposed procedure actually needs no separation of these drugs from their sources before analysis and was unaffected by interference of other phenolic compounds. The proposed method is simpler and faster than the repoeted ones.

  3. Ecology of aerobic anoxygenic phototrophic bacteria along an oligotrophic gradient in the Mediterranean Sea

    D. Lamy

    2011-01-01

    Full Text Available Aerobic anoxygenic phototrophic (AAP bacteria are photoheterotrophic prokaryotes able to use both light and organic substrates for energy production. They are widely distributed in coastal and oceanic environments and may contribute significantly to the carbon cycle in the upper ocean. To better understand questions regarding links between the ecology of these photoheterotrophic bacteria and the trophic status of water masses, we examined their horizontal and vertical distribution and the effects of nutrient additions on their growth along an oligotrophic gradient in the Mediterranean Sea. Concentrations of bacteriochlorophyll-a (BChl-a and AAP bacterial abundance decreased from the western to the eastern basins of the Mediterranean Sea and were linked with concentrations of chlorophyll-a, nutrient and dissolved organic carbon. Inorganic nutrient and glucose additions to surface seawater samples along the oligotrophic gradient revealed that AAP bacteria were nitrogen- and carbon-limited in the ultra-oligotrophic eastern basin. The intensity of the AAP bacterial growth response generally differed from that of the total bacterial growth response. BChl-a quota of AAP bacterial communities was significantly higher in the eastern basin than in the western basin, suggesting that reliance on phototrophy varied along the oligotrophic gradient and that nutrient and/or carbon limitation favors BChl-a synthesis.

  4. Ecology of aerobic anoxygenic phototrophic bacteria along an oligotrophic gradient in the Mediterranean Sea

    D. Lamy

    2011-04-01

    Full Text Available Aerobic anoxygenic phototrophic (AAP bacteria are photoheterotrophic prokaryotes able to use both light and organic substrates for energy production. They are widely distributed in coastal and oceanic environments and may contribute significantly to the carbon cycle in the upper ocean. To better understand questions regarding links between the ecology of these photoheterotrophic bacteria and the trophic status of water masses, we examined their horizontal and vertical distribution and the effects of nutrient additions on their growth along an oligotrophic gradient in the Mediterranean Sea. Concentrations of bacteriochlorophyll-a (BChl-a and AAP bacterial abundance decreased from the western to the eastern basin of the Mediterranean Sea and were linked with concentrations of chlorophyll-a, nutrient and dissolved organic carbon. Inorganic nutrient and glucose additions to surface seawater samples along the oligotrophic gradient revealed that AAP bacteria were nitrogen- and carbon-limited in the ultraoligotrophic eastern basin. The intensity of the AAP bacterial growth response generally differed from that of the total bacterial growth response. BChl-a quota of AAP bacterial communities was significantly higher in the eastern basin than in the western basin, suggesting that reliance on phototrophy varied along the oligotrophic gradient and that nutrient and/or carbon limitation favors BChl-a synthesis.

  5. Atypical antipsychotics in the treatment of early-onset schizophrenia

    Hrdlicka M

    2015-04-01

    Full Text Available Michal Hrdlicka, Iva Dudova Department of Child Psychiatry, Charles University Second Faculty of Medicine and University Hospital Motol, Prague, Czech Republic Abstract: Atypical antipsychotics (AAPs have been successfully used in early-onset schizophrenia (EOS. This review summarizes the randomized, double-blind, controlled studies of AAPs in EOS, including clozapine, risperidone, olanzapine, aripiprazole, paliperidone, quetiapine, and ziprasidone. No significant differences in efficacy between AAPs were found, with the exception of clozapine and ziprasidone. Clozapine demonstrated superior efficacy in treatment-resistant patients with EOS, whereas ziprasidone failed to demonstrate efficacy in the treatment of EOS. Our review also focuses on the onset of action and weight gain associated with AAPs. The data on onset of action of AAPs in pediatric psychiatry are scanty and inconsistent. Olanzapine appears to cause the most significant weight gain in patients with EOS, while ziprasidone and aripiprazole seem to cause the least. Keywords: early-onset schizophrenia, atypical antipsychotics, efficacy, onset of action, weight gain

  6. Effects of switching to aripiprazole from current atypical antipsychotics on subsyndromal symptoms and tolerability in patients with bipolar disorder.

    Woo, Young Sup; Bahk, Won-Myong; Park, Young-Min; Chung, Sangkeun; Yoon, Bo-Hyun; Won, Seunghee; Lee, Jeong Goo; Lee, Hwang-Bin; Kim, Won; Jeong, Jong-Hyun; Lee, Kwanghun; Kim, Moon-Doo

    2016-09-01

    We evaluated the effectiveness of aripiprazole among bipolar patients who had switched to this medication as a result of difficulty maintaining on their prestudy atypical antipsychotics (AAPs) because of subsyndromal mood symptoms or intolerance. This study included 77 bipolar patients who were in syndromal remission with an AAP as monotherapy or with an AAP combined with a mood stabilizer(s) who needed to switch from their present AAP because of subsyndromal symptoms or intolerance. At 24 weeks after switching to aripiprazole, the remission rates on the Montgomery-Åsberg Depression Rating Scale (MADRS) and on both the MADRS and the Young Mania Rating Scale were increased significantly in the full sample and in the inefficacy subgroup. In the inefficacy subgroup, the MADRS score change was significant during the 24 weeks of study. Total cholesterol and prolactin decreased significantly after switching to aripiprazole. The proportion of patients who had abnormal values for central obesity and hypercholesterolemia decreased significantly from baseline to week 24. These findings suggest that a change from the current AAP to aripiprazole was associated with improvement in subsyndromal mood symptoms and several lipid/metabolic or safety profile parameters in patients with bipolar disorder with tolerability concerns or subsyndromal mood symptoms. PMID:27487259

  7. Changes in Phosphorylation of Connexin43 in Rats during Acute Myocardial Hypoxia and Effects of Antiarrhythmic Peptide on the Phosphorylation

    WANG Rong; ZHANG Cuntai; RUAN Yanfei; LIU Nian; WANG Lin

    2007-01-01

    In order to confirm the hypothesis that during acute hypoxia, the antiarrhythmic peptide (AAP10) could improve conductance by changing the phosphorylation state of connexin43 (Cx43),isolated perfused rat hearts were randomly divided into three groups: control, hypoxia and AAP10 (n=9 in each group). The change in Cx43 phosphorylation was tested by Western-blot; the distribution of Cx43 was observed by confocal immunofluorescence microscopy. Western-blot analysis revealed that the expression of total Cx43 protein was significantly decreased during acute hypoxia,while nonphosphorylated Cx43 (NP-Cx43) was unchanged. AAP10 could increase the expression of total Cx43 protein, but had no effects on the NP-Cx43 protein. Immunofluorescence study showed that during acute hypoxia, both total Cx43 and NP-Cx43 proteins were greatly decreased, while AAP10 only increased the expression of total Cx43 protein, but had no effect of the NP-Cx43 protein expression. These findings suggested that the decrease of intercellular communication may be associated with the reduction of phosphorylated Cx43 (p-Cx43) and translocation of NP-Cx43 from the surface of gap junction into intracellular pools during acute hypoxia. AAP10 can improve intercelluar communication by enhancing phosphorylation of Cx43.

  8. Growth and characterization of ammonium acid phthalate single crystals

    Arunkumar, A.; Ramasamy, P.

    2013-04-01

    Ammonium acid phthalate (AAP) has been synthesized and single crystals were grown by slow evaporation solution growth technique. The unit cell parameters were confirmed by single crystal X-ray diffraction analysis and it belongs to orthorhombic system with the space group of Pcab. The high resolution X-ray diffraction studies revealed the crystalline perfection of the grown crystal. The various functional groups of AAP were identified by FT-IR and Raman spectral analyses. Thermal stability of the grown crystals was studied by TGA/DTA. The optical properties of the grown crystals were analyzed by UV-Vis-NIR and photoluminescence spectral studies. The mechanical property of the grown crystal was studied by Vickers microhardness measurement. The growth features of AAP were analyzed by chemical etching.

  9. Fabrication of multiwalled carbon nanotube-surfactant modified sensor for the direct determination of toxic drug 4-aminoantipyrine☆

    Jayant I. Gowda; Arunkumar T. Buddanavar; Sharanappa T. Nandibewoor

    2015-01-01

    A multi-walled carbon nanotube (MWCNT)-cetyltrimethylammonium bromide (CTAB) surfactant composite modified glassy carbon electrode (GCE) was developed as a novel system for the determination of 4-aminoantipyrine(AAP). The oxidation process was irreversible over the pH range studied and exhibited a diffusion controlled behavior. All experimental parameters were optimized. The combination of MWCNT-CTAB endows the biosensor with large surface area, good biological compatibility, electricity and stability, high selectivity and sensitivity. MWCNT-CTAB /GCE electrode gave a linear response for AAP from 5.0 × 10-9 to 4.0 × 10-8 M with a detection limit of 1.63 × 10-10 M. The modified electrode showed good selectivity against interfering species and also exhibited good reproducibility. The present electrochemical sensor based on the MWCNT-CTAB/GCE electrode was applied to the determination of AAP in real samples.

  10. Lawrence Berkeley Laboratory Affirmative Action Program. Revised

    NONE

    1995-06-01

    The Lawrence Berkeley Laboratory`s Affirmative Action Program (AAP) serves as a working document that describes current policies, practices, and results in the area of affirmative action. It represents the Laboratory`s framework for an affirmative approach to increasing the representation of people of color and women in segments of our work force where they have been underrepresented and taking action to increase the employment of persons with disabilities and special disabled and Vietnam era veterans. The AAP describes the hierarchy of responsibility for Laboratory affirmative action, the mechanisms that exist for full Laboratory participation in the AAP, the policies and procedures governing recruitment at all levels, the Laboratory`s plan for monitoring, reporting, and evaluating affirmative action progress, and a description of special affirmative action programs and plans the Laboratory has used and will use in its efforts to increase the representation and retention of groups historically underrepresented in our work force.

  11. Growth and nutritional status of children from dysfunctional families with alcohol addicted parents in Poland.

    Hanć, Tomasz; Czapla, Zbigniew; Szwed, Anita; Durda, Magdalena; Krotowska, Aleksandra; Cieślik, Joachim

    2015-07-01

    The study was aimed at assessment of impact of parents' alcohol addiction on growth and prevalence of underweight and overweight in their children. Two groups of subjects were compared: 80 children of alcohol addicted parents (ChAAP) aged from 7 to 14 years and reference group (RG) of 1000 children selected in terms of age and place of residence. Differences in z scores for height and Body Mass Index (BMI), prevalence of underweight and overweight were assessed. Families of ChAAP were characterized by: lower parents' education, higher unemployment rate, a greater number of children than in RG. The differences between ChAAP and RG in z scores for height (z scores: -0.54 vs. 0.45, t = -7.01, p bad living conditions, e.g.: chronic post-natal stress, or adverse events during fetal development. PMID:26042531

  12. Solar energy applications at Army ammunition plants

    Lowry, A. P.; Moy, S. M.

    1982-06-01

    The Army Ammunition Plants use significant quantities of fossil fuels. To reduce dependence on these scarce, costly, and non-renewable fuels, a study was conducted to investigate potential solar energy applications at the AAPs. Solar energy is a low-level energy source which is best applied to low temperature applications. It can be used at the AAPs to preheat boiler feedwater, provide hot air for dry-houses, provide domestic hot water and heat for administration buildings, and provide hot water for manufacturing processes such as metal cleaning, phosphating, and X-ray film processing. Use of the flat plate collectors, evacuated tube collectors, or solar ponds with the possible addition of a heat pump, offers reasonably economical means of applying solar technology to AAP needs.

  13. Pro-oxidant status and matrix metalloproteinases in apical lesions and gingival crevicular fluid as potential biomarkers for asymptomatic apical periodontitis and endodontic treatment response

    Dezerega Andrea

    2012-03-01

    Full Text Available Abstract Background Oxidative stress and matrix metalloproteinases -9 and -2 are involved in periodontal breakdown, whereas gingival crevicular fluid has been reported to reflect apical status. The aim of this study was to characterize oxidant balance and activity levels of MMP -2 and -9 in apical lesions and healthy periodontal ligament; and second, to determine whether potential changes in oxidant balance were reflected in gingival crevicular fluid from asymptomatic apical periodontitis (AAP-affected teeth at baseline and after endodontic treatment. Methods Patients with clinical diagnosis of AAP and healthy volunteers having indication of tooth extraction were recruited. Apical lesions and healthy periodontal ligaments, respectively, were homogenized or processed to obtain histological tissue sections. Matrix metalloproteinase -9 and -2 levels and/or activity were analyzed by Immunowestern blot, zymography and consecutive densitometric analysis, and their tissue localization was confirmed by immunohistochemistry. A second group of patients with AAP and indication of endodontic treatment was recruited. Gingival crevicular fluid was extracted from AAP-affected teeth at baseline, after endodontic treatment and healthy contralateral teeth. Total oxidant and antioxidant status were determined in homogenized tissue and GCF samples. Statistical analysis was performed using STATA v10 software with unpaired t test, Mann-Whitney test and Spearman's correlation. Results Activity of MMP-2 and MMP-9 along with oxidant status were higher in apical lesions (p Conclusions Apical lesions display an oxidant imbalance along with increased activity of matrix metalloproteinase-2 and -9 and might contribute to AAP progression. Oxidant imbalance can also be reflected in GCF from AAP-affected teeth and was restored to normal levels after conservative endodontic treatment. These mediators might be useful as potential biomarkers for chair-side complementary diagnostic

  14. Active appearance pyramids for object parametrisation and fitting.

    Zhang, Qiang; Bhalerao, Abhir; Dickenson, Edward; Hutchinson, Charles

    2016-08-01

    Object class representation is one of the key problems in various medical image analysis tasks. We propose a part-based parametric appearance model we refer to as an Active Appearance Pyramid (AAP). The parts are delineated by multi-scale Local Feature Pyramids (LFPs) for superior spatial specificity and distinctiveness. An AAP models the variability within a population with local translations of multi-scale parts and linear appearance variations of the assembly of the parts. It can fit and represent new instances by adjusting the shape and appearance parameters. The fitting process uses a two-step iterative strategy: local landmark searching followed by shape regularisation. We present a simultaneous local feature searching and appearance fitting algorithm based on the weighted Lucas and Kanade method. A shape regulariser is derived to calculate the maximum likelihood shape with respect to the prior and multiple landmark candidates from multi-scale LFPs, with a compact closed-form solution. We apply the 2D AAP on the modelling of variability in patients with lumbar spinal stenosis (LSS) and validate its performance on 200 studies consisting of routine axial and sagittal MRI scans. Intervertebral sagittal and parasagittal cross-sections are typically used for the diagnosis of LSS, we therefore build three AAPs on L3/4, L4/5 and L5/S1 axial cross-sections and three on parasagittal slices. Experiments show significant improvement in convergence range, robustness to local minima and segmentation precision compared with Constrained Local Models (CLMs), Active Shape Models (ASMs) and Active Appearance Models (AAMs), as well as superior performance in appearance reconstruction compared with AAMs. We also validate the performance on 3D CT volumes of hip joints from 38 studies. Compared to AAMs, AAPs achieve a higher segmentation and reconstruction precision. Moreover, AAPs have a significant improvement in efficiency, consuming about half the memory and less than 10% of

  15. Structural, thermal, morphological and biological studies of proton-transfer complexes formed from 4-aminoantipyrine with quinol and picric acid

    Adam, Abdel Majid A.

    2013-03-01

    4-Aminoantipyrine (4AAP) is widely used in the pharmaceutical industry, biochemical experiments and environmental monitoring. However, residual amounts of 4AAP in the environment may pose a threat to human health. To provide basic data that can be used to extract or eliminate 4AAP from the environment, the proton-transfer complexes of 4AAP with quinol (QL) and picric acid (PA) were synthesized and spectroscopically investigated. The interactions afforded two new proton-transfer salts named 1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-aminium-4-hydroxyphenolate and 1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-aminium-2,4,6-trinitrophenolate for QL and PA, respectively, via a 1:1 stoichiometry. Elemental analysis (CHN), electronic absorption, spectrophotometric titration, IR, Raman, 1H NMR and X-ray diffraction were used to characterize the new products. The thermal stability of the synthesized CT complexes was investigated using thermogravimetric (TG) analyses, and the morphology and particle size of these complexes were obtained from scanning electron microscopy (SEM). It was found that PA and 4AAP immediately formed a yellow precipitate with a remarkable sponge-like morphology and good thermal stability up to 180 °C. Finally, the biological activities of the newly synthesized CT complexes were tested for their antibacterial and antifungal activities. The results indicated that the [(4AAP)(QL)] complex exhibited strong antimicrobial activities against various bacterial and fungal strains compared with standard drugs.

  16. Precipitation estimation in mountainous terrain using multivariate geostatistics. Part II: isohyetal maps

    Hevesi, Joseph A.; Flint, Alan L.; Istok, Jonathan D.

    1992-01-01

    Values of average annual precipitation (AAP) may be important for hydrologic characterization of a potential high-level nuclear-waste repository site at Yucca Mountain, Nevada. Reliable measurements of AAP are sparse in the vicinity of Yucca Mountain, and estimates of AAP were needed for an isohyetal mapping over a 2600-square-mile watershed containing Yucca Mountain. Estimates were obtained with a multivariate geostatistical model developed using AAP and elevation data from a network of 42 precipitation stations in southern Nevada and southeastern California. An additional 1531 elevations were obtained to improve estimation accuracy. Isohyets representing estimates obtained using univariate geostatistics (kriging) defined a smooth and continuous surface. Isohyets representing estimates obtained using multivariate geostatistics (cokriging) defined an irregular surface that more accurately represented expected local orographic influences on AAP. Cokriging results included a maximum estimate within the study area of 335 mm at an elevation of 7400 ft, an average estimate of 157 mm for the study area, and an average estimate of 172 mm at eight locations in the vicinity of the potential repository site. Kriging estimates tended to be lower in comparison because the increased AAP expected for remote mountainous topography was not adequately represented by the available sample. Regression results between cokriging estimates and elevation were similar to regression results between measured AAP and elevation. The position of the cokriging 250-mm isohyet relative to the boundaries of pinyon pine and juniper woodlands provided indirect evidence of improved estimation accuracy because the cokriging result agreed well with investigations by others concerning the relationship between elevation, vegetation, and climate in the Great Basin. Calculated estimation variances were also mapped and compared to evaluate improvements in estimation accuracy. Cokriging estimation variances

  17. Efficiency and patient experience with propofol vsconventional sedation: A prospective study

    2016-01-01

    AIM To determine whether anaesthesiologistadministeredsedation with propofol (AAP) or endoscopist-administered conscious sedation (EAC) withfentanyl/midazolam shortens colonoscopy duration/totalroom time.METHODS: This is a prospective, non-randomized,comparative study that enrolled patients greater than18 years of age undergoing colonoscopy in a singleCanadian academic outpatient endoscopy unit over athree-month consecutive period. Colonoscopies in thisunit are performed both with AAP and EAC. Patientdemographics, procedure-related data and adverseevents were documented. Additionally, the level ofprocedure difficulty, and whether a staff endoscopist,trainee with assistance, or independent trainee, performedthe procedure were documented. A validatedmodified 4-question, 5-point Likert scale telephonesurvey was used to assess patient satisfaction withcolonoscopy. The telephone patient satisfaction surveywas conducted 24-72 h following the procedure.RESULTS: Two hundred and thirty patients were enrolled during the study period with 126 patients inthe AAP group and 104 patients in the EAC group.Mean procedure time was 18.3 ± 10.1 min in theAAP group and 14.7 ± 7.1 min in the EAC group (P =0.002). Mean total room time was 36.8 ± 13.7 with AAPand 30.1 ± 11 min with EAC (p 〈 0.001). Multivariateanalysis revealed the use of AAP (P = 0.002), residentparticipation (p 〈 0.001), diagnostic interventions (p= 0.033), therapeutic interventions (p 〈 0.001), lowerbody mass index (p = 0.008) and American Society ofAnaesthesiologist class (p = 0.016), to be predictorsof longer total room time. Patient age and genderwere not significant predictors. After excluding cases inwhich trainees were involved, there was no significantdifference in procedure time between the two groups (p= 0.941), however total room time was still prolongedin the AAP group (p = 0.019). The amount of painexperienced was lower with AAP (p = 0.02

  18. Advanced Avionics and Processor Systems for Space and Lunar Exploration

    Keys, Andrew S.; Adams, James H.; Ray, Robert E.; Johnson, Michael A.; Cressler, John D.

    2009-01-01

    NASA's newly named Advanced Avionics and Processor Systems (AAPS) project, formerly known as the Radiation Hardened Electronics for Space Environments (RHESE) project, endeavors to mature and develop the avionic and processor technologies required to fulfill NASA's goals for future space and lunar exploration. Over the past year, multiple advancements have been made within each of the individual AAPS technology development tasks that will facilitate the success of the Constellation program elements. This paper provides a brief review of the project's recent technology advancements, discusses their application to Constellation projects, and addresses the project's plans for the coming year.

  19. Fluorescence excitation spectrum and solvent-assisted conformational isomerization (SACI) of jet-cooled acetaminophen

    Sohn, Woon Yong; Kang, Jeong Seok; Lee, So Young; Kang, Hyuk

    2013-08-01

    Fluorescence excitation spectrum of jet-cooled acetaminophen was obtained. When AAP was expanded with a buffer gas containing 0.3-1.1% of water, absorption peaks of the less stable trans conformer was significantly reduced by solvent-assisted conformational isomerization (SACI), which is confirmed by a separately measured UV-UV hole burning spectroscopy. It is also confirmed by quantum mechanical calculation and RRKM calculation that it is energetically and kinetically possible to induce SACI in AAP with water. The SACI mechanism suggests a possible pathway that acetaminophen can adopt an active conformation in vivo, which is need for molecular recognition and drug activity.

  20. NCBI nr-aa BLAST: CBRC-TSYR-01-1170 [SEVENS

    Full Text Available CBRC-TSYR-01-1170 ref|NP_898745.1| putative cadmium resistance protein (CadA) [Rhod...ococcus erythropolis] gb|AAP74015.1| putative cadmium resistance protein (CadA) [Rhodococcus erythropolis] NP_898745.1 0.14 29% ...

  1. Study of the behaviour of various phenolic compounds in the 4-aminoantipyrine and ultraviolet-ratio spectrophotometric methods without and with distillation.

    Farino, J; Norwitz, G; Boyko, W J; Keliher, P N

    1981-09-01

    It is customary in industrial analysis in the determination of phenols by the 4-aminoantipyrine (4-AAP) and ultraviolet-ratio spectrophotometric methods to report the total of phenolic compounds as phenol. A study was therefore made of the behaviour of 36 representative phenolic compounds in the 4-AAP and UV-ratio methods, with and without distillation, to ascertain the apparent recoveries relative to that for phenol. The Fisher phenol analyser was used for the UV-ratio method, which depends upon the bathochromic shift (from about 270 to about 290 nm) usually obtained when the solution of the phenol is made alkaline. The apparent recoveries by the 4-AAP method both with and without distillation varied from 0 to 100%. The apparent recoveries by the UV-ratio method without distillation varied from 0 to 148%, and those with distillation varied from 0 to 110%. Sixteen of the compounds tested without distillation gave less than 10% recovery by the 4-AAP method and eleven gave less than 10% recovery by the UV-ratio method. The results after distillation indicated that several of the compounds did not distil completely. PMID:18962987

  2. Influence of the surface structure on the filtration performance of UV-modified PES membranes

    Kæselev, Bozena Alicja; Kingshott, P.; Jonsson, Gunnar Eigil

    2002-01-01

    Poly (ether sulfone) (PES) 50 kDa membranes were surface modified by irradiation with UV light (254 nm) in the presence of N-vinyl-2-pyrrolidine (NVP), 2-acrylamidoglycolic acid monohydrate (AAG) and 2-acrylamido-2-methyl-1-propanesulfonic acid (AAP). The surfaces of the modified membranes were c...

  3. Photoheterotrophic Microbes in the Arctic Ocean in Summer and Winter▿

    Cottrell, Matthew T.; Kirchman, David L.

    2009-01-01

    Photoheterotrophic microbes, which are capable of utilizing dissolved organic materials and harvesting light energy, include coccoid cyanobacteria (Synechococcus and Prochlorococcus), aerobic anoxygenic phototrophic (AAP) bacteria, and proteorhodopsin (PR)-containing bacteria. Our knowledge of photoheterotrophic microbes is largely incomplete, especially for high-latitude waters such as the Arctic Ocean, where photoheterotrophs may have special ecological relationships and distinct biogeochem...

  4. Unigene BLAST: CBRC-PMAR-01-0433 [SEVENS

    Full Text Available CBRC-PMAR-01-0433 gnl|UG|Pma#S35051446 PMAD-aap78e15.b1 Lamprey_WGS_pCMV-sport6 Petromyzon marin ... 1193 creatine kinase (EC 2.7.3.2) - African clawed frog ... (fragment), mRNA sequence /clone_end=3' /gb=DW0204 ...

  5. Unigene BLAST: CBRC-PMAR-01-0107 [SEVENS

    Full Text Available CBRC-PMAR-01-0107 gnl|UG|Pma#S35051349 PMAD-aap76d05.b1 Lamprey_WGS_pCMV-sport6 Petromyzon marin ... e protein precursor (clone X0286) - African clawed frog , mRNA sequence /clone_end=3' /gb=DW020364 /gi=8366 ...

  6. Enhancing School Asthma Action Plans: Qualitative Results from Southeast Minnesota Beacon Stakeholder Groups

    Egginton, Jason S.; Textor, Lauren; Knoebel, Erin; McWilliams, Deborah; Aleman, Marty; Yawn, Barbara

    2013-01-01

    Background: This study explores ways southeast Minnesota schools currently address asthma problems, identifies areas for improvement, and assesses the potential value of asthma action plans (AAPs) in schools. Methods: Focus groups were used to query stakeholder groups on asthma care in schools. Groups were held separately for elementary school…

  7. Recent evidence and potential mechanisms underlying weight gain and insulin resistance due to atypical antipsychotics

    Ana Maria Volpato

    2013-09-01

    Full Text Available Objective: Atypical antipsychotics (AAPs promote obesity and insulin resistance. In this regard, the main objective of this study was to present potential mechanisms and evidence concerning side effects of atypical antipsychotics in humans and rodents. Method: A systematic review of the literature was performed using the MEDLINE database. We checked the references of selected articles, review articles, and books on the subject. Results: This review provides consistent results concerning the side effects of olanzapine (OL and clozapine (CLZ, whereas we found conflicting results related to other AAPs. Most studies involving humans describe the effects on body weight, adiposity, lipid profile, and blood glucose levels. However, it seems difficult to identify an animal model replicating the wide range of changes observed in humans. Animal lineage, route of administration, dose, and duration of treatment should be carefully chosen for the replication of the findings in humans. Conclusions: Patients undergoing treatment with AAPs are at higher risk of developing adverse metabolic changes. This increased risk must be taken into account when making decisions about treatment. The influence of AAPs on multiple systems is certainly the cause of such effects. Specifically, muscarinic and histaminergic pathways seem to play important roles.

  8. A Novel Approach to Critical Congenital Heart Disease (CCHD Screening at Moderate Altitude

    Erin Lueth

    2016-07-01

    Full Text Available The American Academy of Pediatrics (AAP has endorsed Critical Congenital Heart Disease (CCHD screening using pulse oximetry nationwide, but, however, acknowledges that altitude may impact failure rates and alternative algorithms may be required at high altitudes. We therefore evaluated a modified screening protocol at an altitude of 6200 feet with the hypothesis that modifications could decrease failure rates. We evaluated 2001 well, newborn infants ≥35 weeks gestation using a modified protocol, which included a lower saturation cutoff for the first screen (85% instead of the AAP recommended 90% and an oxygen hood intervention between the first two screens. Using our modified screening algorithm, we found a 0.3% failure rate, which was similar to the 0.2% sea-level rate and statistically different from the 1.1% rate identified in a recent study at similar altitude. Had the AAP protocol been used, the failure rate would have increased to 0.8%, which is similar to prior reports near this altitude. Echocardiograms were performed on failing newborns with no CCHD identified. A Birth Defects Registry Database review demonstrated one newborn with CCHD was missed after meeting AAP passing criteria. Overall, this study demonstrates that an alternative algorithm can be implemented at moderate altitude with decreased failure rate and comparable false negative rate.

  9. Rundum artist-run space and its elusive form / Hanna Laura Kaljo, Mari-Leen Kiipli, Kulla Laas ... [jt.

    2015-01-01

    Rundum on 2013. a septembris Tallinnas loodud liikuv loominguline platvorm, mille eesmärgiks on suhestuda erinevate kohtadega, reageerida tühikutele Eesti loomemaastikus ning uurida sealjuures ka omaalgatuslike praktikate võimalikkust kohalikus kontekstis. Vestlusringis Rundumi algatajad Mari-Leen Kiipli, Kulla Laas, Aap Tepper, Mari Volens, Kristina Õllek

  10. Simultaneous Detection of Phenols and Anilines in Oilfield Waste Water

    Yuan Cunguang; Feng Chengwu

    1996-01-01

    @@ Phenols and aromatic anilines are monitored in many countries , because both of them pollute environment seriously. The methods of 4-AAP(4-Aminoantipyrine)photometric detection of volatile phenols and naphthalene -ethyl-diamine-azo photometric detection of anilines are recommended by the National Environmental Protection Bureau, China (NEPBC).

  11. Zirconyl acetaminophen phosphate: A nanoscaled analgetic with very high drug load.

    Heck, Joachim G; Feldmann, Claus

    2016-11-01

    Drug release belongs to the most challenging aspects of nanoparticles addressing molecular biology and medicine. Besides targeted delivery, obvious challenges are related to high drug load and continuous slow drug release. Based on our recently developed concept of inorganic-organic hybrid nanoparticles (IOH-NP), we here present [ZrO](2+)[AAP](2-) IOH-NPs containing the analgetic phosphate prodrug acetaminophen phosphate for drug release. [ZrO](2+)[AAP](2-) combines an uncomplex synthesis in water with a high prodrug load of 68wt.%. [ZrO](2+)[AAP](2-) nanoparticles exhibit a diameter of 37(11)nm and can be readily obtained as colloidally highly stable suspension in water. The chemical composition is studied in detail based on infrared spectroscopy, energy-dispersive X-ray analysis, thermogravimetry and elemental analysis. Moreover, the release of acetaminophen from [ZrO](2+)[AAP](2-) is studied by means of model experiments indicating the carbon content of the nanoparticles and, in alternative, the fluorescence of labeled nanoparticles. Both data show a continuous release of 80wt.% of the analgetic acetaminophen on a time scale up to 48h. PMID:27451036

  12. NCBI nr-aa BLAST: CBRC-MDOM-04-0142 [SEVENS

    Full Text Available CBRC-MDOM-04-0142 ref|YP_073290.1| NADH dehydrogenase subunit 5 [Pachypsylla venust...a] gb|AAP14655.1| NADH dehydrogenase subunit 5 [Pachypsylla venusta] YP_073290.1 0.039 25% ...

  13. Establishing a Book Publishing Curriculum.

    Ciofalo, Andrew

    Addressing the need to prepare college graduates for careers in book publishing, this report examines the necessity and structure of a book publishing curriculum at the undergraduate level at Loyola College in Maryland. A 1977 bulletin by the American Association of Publishers (AAP) cited a lack of awareness of publishing as a possible career, and…

  14. Korsten : tellimata tellised = Chimney : an outside job / Andres Aule

    Aule, Andres

    2012-01-01

    Aap Kaur Suvi kavandatud Tallinna linnainstallatsioonide festivali "LIFT11" installatsioonist "Korsten", mis pidi seisnema Tauno Kangro skulptuuri "Lõbus korstnapühkija" ümber ajutise telliskorstna ehitamises. Lift11 jättis selle installatsiooni ära, kuid ootamatult sai "Korsten" kodanikualgatusena 8. IX 2011 teoks

  15. Gene : CBRC-DYAK-06-0016 [SEVENS

    Full Text Available CBRC-DYAK-06-0016 Novel X A UNKNOWN TRFR_MOUSE 9e-14 35% pir||JC7978 proctolin receptor (CG6986) ... - fruit fly ... (Drosophila melanogaster) gb|AAP50844.1| peptide r ... LIAVVLMFIVCQLPWAIYLIVNQYMEIQVGTQVVAGNVCNLLASLHAASNFFLY CVLSDKYRKTVRELITGYRYRRRHARNNTSLYVPHTTTTLTQINGDHYGSN ...

  16. Gene : CBRC-DSIM-08-0021 [SEVENS

    Full Text Available CBRC-DSIM-08-0021 Novel UN A UNKNOWN OPRM_PANTR 8e-08 26% pir||JC7978 proctolin receptor (CG6986 ... ) - fruit fly ... (Drosophila melanogaster) gb|AAP50844.1| peptide r ... LIAVVLMFIVCQLPWAIYLIVNQYMEIQVGTQVVAGNVCNLLASLHAASNFFLY CVLSDKYRKTVRELITGYRYRRRHARNNTSLYVPHTTTTLTQINGDHYGSN ...

  17. Mechanisms of intramolecular communication in a hyperthermophilic acylaminoacyl peptidase

    Papaleo, Elena; Renzetti, Giulia; Tiberti, Matteo

    2012-01-01

    Protein dynamics and the underlying networks of intramolecular interactions and communicating residues within the three-dimensional (3D) structure are known to influence protein function and stability, as well as to modulate conformational changes and allostery. Acylaminoacyl peptidase (AAP) subf...

  18. Protein assay for heme oxygenase-1 (HO-1) induced by chemicals in HepG2 cells.

    Miyamoto, Yohei; Ohshida, Keiyu; Sasago, Kaori

    2009-12-01

    Levels of heme oxygenase-1 (HO-1), a stress response protein, were measured to examine oxidative stress induced by several chemicals in HepG2 cells with and without S9mix using an ELISA. CdCl(2), heme, and diclofenac sodium salt (diclofenac) were used as inducers of HO-1. Acetaminophen (AAP) and cyclophosphamide (CP) were used as oxidative stress inducers. Stannic mesoporphyrin (SnMP) was used as an inhibitor of HO activity. Cytotoxicity was determined, and HO-1 levels were measured in HepG2 cells exposed to chemicals other than CP with non-metabolic activation without S9mix, and to diclofenac, AAP and CP with metabolic activation with S9mix. HO-1 levels were increased by CdCl(2) (7.5 microM), heme (10, 100 microM), and stannic mesoporphyrin (SnMP) (10 microM), but were not changed by AAP, and were decreased by diclofenac. HO-1 levels were increased by diclofenac (300 microM), and CP (36 microM), but were unaffected by AAP because of low sensitivity in HepG2 cells. The induction of HO-1 expression was first observed in cultured HepG2 cells treated with CP under conditions involving metabolic activation. These results showed the measurement of HO-1 protein levels in this system is useful when assessing oxidative stress as a tool for detecting drug toxicity. PMID:19952508

  19. NCBI nr-aa BLAST: CBRC-DNOV-01-2484 [SEVENS

    Full Text Available CBRC-DNOV-01-2484 ref|YP_006355.1| ATP synthase F0 subunit 6 [Siphonodentalium loba...tum] gb|AAP91676.1| ATP synthase F0 subunit 6 [Siphonodentalium lobatum] YP_006355.1 5e-04 29% ...

  20. Gay and Lesbian Parents

    ... may benefit from meeting other children who have gay or lesbian parents. You might find a local group of families, or your children might be interested in joining an e-mail list or finding a pen pal. Civil marriage. The AAP supports civil marriage for all parents. ...

  1. Vacuum mechatronic laser alignment system on the Nova laser

    The experiments conducted on NOVA are done to investigate inertially confined laser fusion reactions. To this end, the ten beams of the laser are aligned to within 30mm. The target chamber employs a vacuum mechatronic based reticle/target positioning system to accomplish this. It is a five degree-of-freedom chamber resident system, known as the Alignment Aids Positioner or AAP. The AAP aids in beam and diagnostic alignment by accurately positioning a reticle at target chamber center to with 7mm. The AAP system increases target positioning and alignment flexibility and accuracy through the use of a computer controlled multi degree-of-freedom stage assembly. This device uses microstepping DC stepper motors with encoders to achieve closed loop control in a 10-6 torr vacuum. The AAP has two positioning regimes to move the alignment reticle and do beam alignment. One is course positioning in the Y-Z plane that moves a high resolution stage assembly to target chamber center. The other regime is high resolution movement in the X,Y,Z and q directions. 5 refs., 9 figs

  2. NCBI nr-aa BLAST: CBRC-TGUT-17-0007 [SEVENS

    Full Text Available CBRC-TGUT-17-0007 ref|NP_846391.1| cytochrome c oxidase, subunit I [Bacillus anthracis str. Ames ... ome c oxidase, subunit i [Bacillus anthracis str. 'Ames ... Ancestor'] gb|AAP27877.1| cytochrome c oxidase, su ... bunit I [Bacillus anthracis str. Ames ] gb|AAT33275.1| cytochrome c oxidase, subunit I [B ...

  3. NCBI nr-aa BLAST: CBRC-CREM-01-1294 [SEVENS

    Full Text Available CBRC-CREM-01-1294 ref|NP_847439.1| major facilitator family transporter [Bacillus anthracis str. ... Ames ] ref|YP_021916.2| major facilitator family transpo ... rter [Bacillus anthracis str. 'Ames ... Ancestor'] gb|AAP28925.1| major facilitator family ...

  4. NCBI nr-aa BLAST: CBRC-DYAK-04-0025 [SEVENS

    Full Text Available CBRC-DYAK-04-0025 ref|NP_834435.1| Spore germination protein IA [Bacillus cereus AT...CC 14579] gb|AAP11636.1| Spore germination protein IA [Bacillus cereus ATCC 14579] NP_834435.1 0.044 23% ...

  5. 75 FR 33321 - Federal Property Suitable as Facilities To Assist the Homeless

    2010-06-11

    ...: Underutilized Reasons: Secured Area Tennessee Bldgs. ZZ001, ZZ002 Milan AAP Milan TN 38358 Landholding Agency...: Secured Area District of Columbia Bldg. 51 Fort McNair Washington, DC Landholding Agency: Army Property Number: 21201020005 Status: Unutilized Reasons: Secured Area and Extensive deterioration Georgia...

  6. 75 FR 70941 - Federal Property Suitable as Facilities To Assist the Homeless

    2010-11-19

    ..., 7006, 7051, 7202, 7814, 8064, MM001 Reasons: Secured Area 4 Bldgs. Milan AAP Gibson TN 38358...: Unutilized Directions: 100, 338, 343, 385, 411, 412, 413, 486, 489, 490, 491, 493, 5006 Reasons: Secured Area...: 21201040004 Status: Unutilized Reasons: Secured Area Hawaii Bldg. 1000 Wheeler Army Airfield Wahiawa HI...

  7. 78 FR 71635 - Federal Property Suitable as Facilities To Assist the Homeless

    2013-11-29

    ...: Secured Area Tennessee 4 Buildings Milan AAP Milan TN 38358 Landholding Agency: Army Property Number.... ft.; storage; lead paint; dropping; secured area; contact Agriculture for more info. Imperial Dam....; stored targets; 66+ yrs.-old; extensive exterior/interior wood damage; asbestos/lead; secured...

  8. NCBI nr-aa BLAST: CBRC-DNOV-01-1344 [SEVENS

    Full Text Available CBRC-DNOV-01-1344 ref|YP_223898.1| putative tape measure protein [Lactobacillus pha...ge phiJL-1] gb|AAP74525.1| putative tape measure protein [Lactobacillus plantarum bacteriophage phiJL-1] YP_223898.1 0.015 22% ...

  9. Gene : CBRC-CINT-01-0007 [SEVENS

    Full Text Available CBRC-CINT-01-0007 Novel UN A UNKNOWN SSR2_HUMAN 9e-33 29% gb|AAP91736.1| kappa opioid receptor-l ... 0894 BW180279 Nori Satoh unpublished cDNA library, heart ... Ciona intestinalis cDNA clone rciht018k05 3', mRNA ...

  10. Gene : CBRC-CINT-01-0071 [SEVENS

    Full Text Available CBRC-CINT-01-0071 Novel UN A UNKNOWN SSR5_MOUSE 2e-31 30% gb|AAP91736.1| kappa opioid receptor-l ... 0894 BW180279 Nori Satoh unpublished cDNA library, heart ... Ciona intestinalis cDNA clone rciht018k05 3', mRNA ...

  11. Eesti paviljon "Koda" = Estonian pavilion "Koda"

    2015-01-01

    Eesti paviljon "Koda" Hollandi maailmanäitusel "Floriade". Arhitektuurivõistlus 2011. Arhitektid Joel Kopli, Koit Ojaliiv (Kuu Arhitektid), konstruktorid Alar Hammer, Marek Suursalu (Projekt 363). Lauad Kristel Jakobson (Haka Disain), toolid Aap Piho (Maast Furniture), valgustid Margus Triibmann (Keha3)

  12. Well-Child-Care - A Check-Up for Success

    ... a Pediatrician Family Life Medical Home Health Insurance Pediatric Specialists Family Dynamics Media Work & Play Getting Involved in Your Community Healthy Children > Family Life > Medical Home > AAP Schedule of Well-Child Care Visits Family Life Listen Español Text Size Email ...

  13. When to Call Your Pediatrician

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a ... to Call Your Pediatrician Page Content Article Body Is it OK to call my child's pediatrician even if I'm not sure my ...

  14. Phosphorus forms and bioavailability of lake sediments in the middle and lower reaches of Yangtze River

    ZHU; Guangwei; QIN; Boqiang; ZHANG; Lu

    2006-01-01

    Forms of phosphorus in sediments from 25 lakes in the middle and lower reaches of Yangtze River were analyzed by the sequential extraction procedure. Contents and spatial distrubution of algal available phosphorus (AAP) in sediments of Lake Taihu, the third largest freshwater lake of China, were also studied. Relationships between phosphorus forms in sediment and macrophytes coverage in sample sites, as well as phosphorus forms in sediments and chlorophyal contents in lake water were discussed. Exchangeable form of phosphorus (Ex-P) in surface sediments was significantly positive correlative to total phosphorus (TP), dissolved total phosphorus (DTP) and soluble reactive phosphorus (SRP) contents in the lake water. Bioavailable phosphorus (Bio-P) contents in sediments from macrophytes dominant sites were significantly lower than that in no macrophyte sites. In Lake Taihu, Ex-P content in top 3 cm sediment was highest.However, content of ferric fraction phosphorus (Fe-P) was highest in 4-10 cm. Bioavalilble phosphorus (Bio-P) contents in surface sediments positively correlated to Chlorophyll a contents in water of Lake Taihu with significant difference. Therefore, contents of Bio-P and AAP could be acted as the indicators of risks of internal release of phosphorus in the shallow lakes. It was estimated that there were 268.6 ton AAP in top 1 cm sediments in Lake Taihu. Sediment suspension caused by strong wind-induced wave disturbance could carry plenty of AAP into water in large shallow lakes like Lake Taihu.

  15. Toddler Growth and Development

    ... in Action Medical Editor & Editorial Advisory Board Sponsors Sponsorship Opporunities Spread the Word Shop AAP Find a ... Body Your child is advancing from infancy toward and into the preschool years. During this time, his or her physical growth and motor development will slow, but you can expect to see ...

  16. Bemisia tabaci (Homoptera: Aleyrodidae) and Indian cassava mosaic virus transmission

    Bemisia tabaci (Gennadius) adults from colonies reared on cassava or sweet potato plants were studied to determine their ability to transmit Indian cassava mosaic virus (ICMV) (Geminiviridae: Begomovirus) from cassava to cassava. Virus acquisition access (feeding) periods (AAP) of 48 h on ICMV-infec...

  17. 40 CFR Appendix A to Part 419 - Processes Included in the Determination of BAT Effluent Limitations for Total Chromium...

    2010-07-01

    ... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Processes Included in the Determination of BAT Effluent Limitations for Total Chromium, Hexavalent Chromium, and Phenolic Compounds (4AAP) A...—Processes Included in the Determination of BAT Effluent Limitations for Total Chromium, Hexavalent...

  18. 75 FR 16188 - Work Reserved for Performance by Federal Government Employees

    2010-03-31

    ... 92-1. 57 FR 45096 (September 30, 1992). Most public commenters expressed general satisfaction with....gov/comp/aap/documents/Chapter6.pdf . The Panel concluded, among other things, that `` here is a need... governmental functions and criteria for identifying critical functions. See 74 FR 25775 (May 29, 2009) for...

  19. EST Table: BP124138 [KAIKOcDNA[Archive

    Full Text Available BP124138 epV31947 10/09/28 100 %/171 aa gb|ACN86367.1| troponin T transcript variant B [Bombyx m ... 43 %/179 aa F53A9.10b#CE34878#WBGene00006587#locus:tnt - 2#status:Confirmed#UniProt:Q7Z071#protein_id:AAP6 ...

  20. EST Table: BP123228 [KAIKOcDNA[Archive

    Full Text Available BP123228 epV30814 10/09/28 84 %/191 aa gb|ACN86367.1| troponin T transcript variant B [Bombyx ma ... 37 %/199 aa F53A9.10b#CE34878#WBGene00006587#locus:tnt - 2#status:Confirmed#UniProt:Q7Z071#protein_id:AAP6 ...

  1. EST Table: BP123162 [KAIKOcDNA[Archive

    Full Text Available BP123162 epV30654 10/09/28 99 %/215 aa gb|ACN86367.1| troponin T transcript variant B [Bombyx ma ... 43 %/188 aa F53A9.10b#CE34878#WBGene00006587#locus:tnt - 2#status:Confirmed#UniProt:Q7Z071#protein_id:AAP6 ...

  2. EST Table: BB983523 [KAIKOcDNA[Archive

    Full Text Available BB983523 ovS3021F10r 10/09/28 100 %/181 aa gb|ACN86367.1| troponin T transcript variant B [Bomby ... 44 %/181 aa F53A9.10b#CE34878#WBGene00006587#locus:tnt - 2#status:Confirmed#UniProt:Q7Z071#protein_id:AAP6 ...

  3. EST Table: BP123798 [KAIKOcDNA[Archive

    Full Text Available BP123798 epV31491 10/09/28 81 %/238 aa gb|ACN86367.1| troponin T transcript variant B [Bombyx ma ... 44 %/181 aa F53A9.10b#CE34878#WBGene00006587#locus:tnt - 2#status:Confirmed#UniProt:Q7Z071#protein_id:AAP6 ...

  4. EST Table: BB983162 [KAIKOcDNA[Archive

    Full Text Available BB983162 ovS3016G07r 10/09/28 100 %/185 aa gb|ACN86367.1| troponin T transcript variant B [Bomby ... 43 %/188 aa F53A9.10b#CE34878#WBGene00006587#locus:tnt - 2#status:Confirmed#UniProt:Q7Z071#protein_id:AAP6 ...

  5. EST Table: BB983418 [KAIKOcDNA[Archive

    Full Text Available BB983418 ovS3020D10r 10/09/28 87 %/217 aa gb|ACN86367.1| troponin T transcript variant B [Bombyx ... 44 %/181 aa F53A9.10b#CE34878#WBGene00006587#locus:tnt - 2#status:Confirmed#UniProt:Q7Z071#protein_id:AAP6 ...

  6. EST Table: BB982235 [KAIKOcDNA[Archive

    Full Text Available BB982235 ovS3003H01r 10/09/28 100 %/166 aa gb|ACN86367.1| troponin T transcript variant B [Bomby ... 48 %/152 aa F53A9.10b#CE34878#WBGene00006587#locus:tnt - 2#status:Confirmed#UniProt:Q7Z071#protein_id:AAP6 ...

  7. EST Table: BP122714 [KAIKOcDNA[Archive

    Full Text Available BP122714 epV30144 10/09/28 100 %/158 aa gb|ACN86367.1| troponin T transcript variant B [Bombyx m ... 42 %/166 aa F53A9.10b#CE34878#WBGene00006587#locus:tnt - 2#status:Confirmed#UniProt:Q7Z071#protein_id:AAP6 ...

  8. EST Table: FS866570 [KAIKOcDNA[Archive

    Full Text Available FS866570 E_FL_fner_31I04_R_0 10/09/28 100 %/218 aa gb|ACN86367.1| troponin T transcript variant ... 44 %/181 aa F53A9.10b#CE34878#WBGene00006587#locus:tnt - 2#status:Confirmed#UniProt:Q7Z071#protein_id:AAP6 ...

  9. EST Table: NM_001046756 [KAIKOcDNA[Archive

    Full Text Available NM_001046756 LOC692913 10/09/29 86 %/309 aa ref|NP_001040221.1| troponin T [Bombyx mori] gb|ABD3 ... 44 %/181 aa F53A9.10b#CE34878#WBGene00006587#locus:tnt - 2#status:Confirmed#UniProt:Q7Z071#protein_id:AAP6 ...

  10. NCBI nr-aa BLAST: CBRC-MLUC-01-0132 [SEVENS

    Full Text Available CBRC-MLUC-01-0132 gb|AAP36253.1| Homo sapiens MAP /microtubule affinity-regulating kinase 2 [synt ... hetic construct] gb|AAX29163.1| MAP /microtubule affinity-regulating kinase 2 [syntheti ... c construct] gb|AAX29164.1| MAP /microtubule affinity-regulating kinase 2 [syntheti ...

  11. NCBI nr-aa BLAST: CBRC-MLUC-01-0105 [SEVENS

    Full Text Available CBRC-MLUC-01-0105 gb|AAP36253.1| Homo sapiens MAP /microtubule affinity-regulating kinase 2 [synt ... hetic construct] gb|AAX29163.1| MAP /microtubule affinity-regulating kinase 2 [syntheti ... c construct] gb|AAX29164.1| MAP /microtubule affinity-regulating kinase 2 [syntheti ...

  12. NCBI nr-aa BLAST: CBRC-MLUC-01-0105 [SEVENS

    Full Text Available CBRC-MLUC-01-0105 gb|AAP36006.1| MAP /microtubule affinity-regulating kinase 2 [Homo sapiens] gb| ... AAX32569.1| MAP /microtubule affinity-regulating kinase 2 [syntheti ... c construct] gb|AAX32570.1| MAP /microtubule affinity-regulating kinase 2 [syntheti ...

  13. NCBI nr-aa BLAST: CBRC-MLUC-01-0358 [SEVENS

    Full Text Available CBRC-MLUC-01-0358 gb|AAP36006.1| MAP /microtubule affinity-regulating kinase 2 [Homo sapiens] gb| ... AAX32569.1| MAP /microtubule affinity-regulating kinase 2 [syntheti ... c construct] gb|AAX32570.1| MAP /microtubule affinity-regulating kinase 2 [syntheti ...

  14. NCBI nr-aa BLAST: CBRC-MLUC-01-0358 [SEVENS

    Full Text Available CBRC-MLUC-01-0358 gb|AAP36253.1| Homo sapiens MAP /microtubule affinity-regulating kinase 2 [synt ... hetic construct] gb|AAX29163.1| MAP /microtubule affinity-regulating kinase 2 [syntheti ... c construct] gb|AAX29164.1| MAP /microtubule affinity-regulating kinase 2 [syntheti ...

  15. Using Digital Materials in Online Courses: A Cautionary Tale of Georgia State University

    Talab, Rosemary

    2008-01-01

    On April 15, 2008, a lawsuit was filed against Georgia State University by Cambridge University Press, Oxford University Press, and Sage, supported by the American University Presses (AAP). The complaint asserted ""pervasive, flagrant, and ongoing" unauthorized distribution of copyrighted materials...through its electronic course reserves service,…

  16. Diversity of cultivated and metabolically active aerobic anoxygenic phototrophic bacteria along an oligotrophic gradient in the Mediterranean Sea

    C. Jeanthon

    2011-05-01

    Full Text Available Aerobic anoxygenic phototrophic (AAP bacteria play significant roles in the bacterioplankton productivity and biogeochemical cycles of the surface ocean. In this study, we applied both cultivation and mRNA-based molecular methods to explore the diversity of AAP bacteria along an oligotrophic gradient in the Mediterranean Sea in early summer 2008. Colony-forming units obtained on three different agar media were screened for the production of bacteriochlorophyll-a (BChl-a, the light-harvesting pigment of AAP bacteria. BChl-a-containing colonies represented a low part of the cultivable fraction. In total, 52 AAP strains were isolated and the phylogenetic analyses based on their 16S rRNA and pufM genes showed that they were all affiliated to the Alphaproteobacteria. The most frequently isolated strains belonged to Citromicrobium bathyomarinum, and Erythrobacter and Roseovarius species. Most other isolates were related to species not reported to produce BChl-a and/or may represent novel taxa. Direct extraction of RNA from seawater samples enabled the analysis of the expression of pufM, the gene coding for the M subunit of the reaction centre complex of aerobic anoxygenic photosynthesis. Clone libraries of pufM gene transcripts revealed that most phylotypes were highly similar to sequences previously recovered from the Mediterranean Sea and a large majority (~94% was affiliated with the Gammaproteobacteria. The most abundantly detected phylotypes occurred in the western and eastern Mediterranean basins. However, some were exclusively detected in the eastern basin, reflecting the highest diversity of pufM transcripts observed in this ultra-oligotrophic region. To our knowledge, this is the first study to document extensively the diversity of AAP isolates and to unveil the active AAP community in an oligotrophic marine environment. By pointing out the

  17. Diversity of cultivated and metabolically active aerobic anoxygenic phototrophic bacteria along an oligotrophic gradient in the Mediterranean Sea

    C. Jeanthon

    2011-07-01

    Full Text Available Aerobic anoxygenic phototrophic (AAP bacteria play significant roles in the bacterioplankton productivity and biogeochemical cycles of the surface ocean. In this study, we applied both cultivation and mRNA-based molecular methods to explore the diversity of AAP bacteria along an oligotrophic gradient in the Mediterranean Sea in early summer 2008. Colony-forming units obtained on three different agar media were screened for the production of bacteriochlorophyll-a (BChl-a, the light-harvesting pigment of AAP bacteria. BChl-a-containing colonies represented a low part of the cultivable fraction. In total, 54 AAP strains were isolated and the phylogenetic analyses based on their 16S rRNA and pufM genes showed that they were all affiliated to the Alphaproteobacteria. The most frequently isolated strains belonged to Citromicrobium bathyomarinum, and Erythrobacter and Roseovarius species. Most other isolates were related to species not reported to produce BChl-a and/or may represent novel taxa. Direct extraction of RNA from seawater samples enabled the analysis of the expression of pufM, the gene coding for the M subunit of the reaction centre complex of aerobic anoxygenic photosynthesis. Clone libraries of pufM gene transcripts revealed that most phylotypes were highly similar to sequences previously recovered from the Mediterranean Sea and a large majority (~94 % was affiliated to the Gammaproteobacteria. The most abundantly detected phylotypes occurred in the western and eastern Mediterranean basins. However, some were exclusively detected in the eastern basin, reflecting the highest diversity of pufM transcripts observed in this ultra-oligotrophic region. To our knowledge, this is the first study to document extensively the diversity of AAP isolates and to unveil the active AAP community in an oligotrophic marine environment. By pointing out the discrepancies

  18. Premature adrenarche: etiology, clinical findings, and consequences.

    Voutilainen, Raimo; Jääskeläinen, Jarmo

    2015-01-01

    Adrenarche means the morphological and functional change of the adrenal cortex leading to increasing production of adrenal androgen precursors (AAPs) in mid childhood, typically at around 5-8 years of age in humans. The AAPs dehydroepiandrosterone (DHEA) and its sulfate conjugate (DHEAS) are the best serum markers of adrenal androgen (AA) secretion and adrenarche. Normal ACTH secretion and action are needed for adrenarche, but additional inherent and exogenous factors regulate AA secretion. Inter-individual variation in the timing of adrenarche and serum concentrations of DHEA(S) in adolescence and adulthood are remarkable. Premature adrenarche (PA) is defined as the appearance of clinical signs of androgen action (pubic/axillary hair, adult type body odor, oily skin or hair, comedones, acne, accelerated statural growth) before the age of 8 years in girls or 9 years in boys associated with AAP concentrations high for the prepubertal chronological age. To accept the diagnosis of PA, central puberty, adrenocortical and gonadal sex hormone secreting tumors, congenital adrenal hyperplasia, and exogenous source of androgens need to be excluded. The individually variable peripheral conversion of circulating AAPs to biologically more active androgens (testosterone, dihydrotestosterone) and the androgen receptor activity in the target tissues are as important as the circulating AAP concentrations as determinants of androgen action. PA has gained much attention during the last decades, as it has been associated with small birth size, the metabolic and polycystic ovarian syndrome (PCOS), and thus with an increased risk for type 2 diabetes and cardiovascular diseases in later life. The aim of this review is to describe the known hormonal changes and their possible regulators in on-time and premature adrenarche, and the clinical features and possible later health problems associating with PA. PMID:24923732

  19. Diverse arrangement of photosynthetic gene clusters in aerobic anoxygenic phototrophic bacteria.

    Qiang Zheng

    Full Text Available BACKGROUND: Aerobic anoxygenic photototrophic (AAP bacteria represent an important group of marine microorganisms inhabiting the euphotic zone of the ocean. They harvest light using bacteriochlorophyll (BChl a and are thought to be important players in carbon cycling in the ocean. METHODOLOGY/PRINCIPAL FINDINGS: Aerobic anoxygenic phototrophic (AAP bacteria represent an important part of marine microbial communities. Their photosynthetic apparatus is encoded by a number of genes organized in a so-called photosynthetic gene cluster (PGC. In this study, the organization of PGCs was analyzed in ten AAP species belonging to the orders Rhodobacterales, Sphingomonadales and the NOR5/OM60 clade. Sphingomonadales contained comparatively smaller PGCs with an approximately size of 39 kb whereas the average size of PGCs in Rhodobacterales and NOR5/OM60 clade was about 45 kb. The distribution of four arrangements, based on the permutation and combination of the two conserved regions bchFNBHLM-LhaA-puhABC and crtF-bchCXYZ, does not correspond to the phylogenetic affiliation of individual AAP bacterial species. While PGCs of all analyzed species contained the same set of genes for bacteriochlorophyll synthesis and assembly of photosynthetic centers, they differed largely in the carotenoid biosynthetic genes. Spheroidenone, spirilloxanthin, and zeaxanthin biosynthetic pathways were found in each clade respectively. All of the carotenoid biosynthetic genes were found in the PGCs of Rhodobacterales, however Sphingomonadales and NOR5/OM60 strains contained some of the carotenoid biosynthetic pathway genes outside of the PGC. CONCLUSIONS/SIGNIFICANCE: Our investigations shed light on the evolution and functional implications in PGCs of marine aerobic anoxygenic phototrophs, and support the notion that AAP are a heterogenous physiological group phylogenetically scattered among Proteobacteria.

  20. Metabolic Disturbances Independent of Body Mass in Patients with Schizophrenia Taking Atypical Antipsychotics

    Lee, Jong Il

    2015-01-01

    Objective Atypical antipsychotic (AAP) treatment is associated with weight gain and metabolic disturbances such as dyslipidemia and dysglycemia. The metabolic disturbances are usually considered to develop secondary to weight gain. We performed the comparison of metabolic disturbances of three AAP group with different risk of metabolic side effect after adjusting for body mass to investigate whether any metabolic disturbances develop independently from body mass index (BMI). Methods This cross-sectional study included 174 subjects with schizophrenia who were on 1) monotherapy with clozapine (CL), olanzapine (OL), or quetiapine (QT) (n=61), 2) monotherapy with risperidone (RSP) (n=89), or 3) monotherapy with aripiprizole (ARP), or ziprasidone (ZPS) (n=24) more than 1 year. Association between the prevalence of metabolic disturbances and groups were analysed using logistic regression after adjusting confounding variables including BMI. Analysese of covariance were used to compare the AAP groups in terms of the levels of metabolic parameters. Results There were significant differences among groups in terms of the prevalence of hypertriglyceridemia (p=0.015), low HDL-cholesterol (p=0.017), and hyperglycemia (p=0.022) after adjusting for BMI. Triglyceride level (p=0.014) and the ratio of triglyceride to HDL-cholesterol (p=0.004) were significantly different among groups after adjusting for BMI. Conclusion In conclusion, metabolic disturbances are significantly different in AAP groups even after adjusting BMI. AAPs may have direct effect on metabolic parameters. Blood lipid and glucose levels should be monitored regularly regardless of whether patients tend to gain weight. PMID:25866526

  1. The Effect of Secretory Factors of Adipose-Derived Stem Cells on Human Keratinocytes

    Soo-Wan Nam

    2012-01-01

    Full Text Available The beneficial effects of adipose-derived stem cell conditioned medium (ADSC-CM on skin regeneration have been reported. Although the mechanism of how ADSC-CM promotes skin regeneration is unclear, ADSC-CM contained various growth factors and it is an excellent raw material for skin treatment. ADSC-CM produced in a hypoxia condition of ADSC—in other words, Advanced Adipose-Derived Stem cell Protein Extract (AAPE—has great merits for skin regeneration. In this study, human primary keratinocytes (HKs, which play fundamental roles in skin tissue, was used to examine how AAPE affects HK. HK proliferation was significantly higher in the experimental group (1.22 μg/mL than in the control group. DNA gene chip demonstrated that AAPE in keratinocytes (p < 0.05 notably affected expression of 290 identified transcripts, which were associated with cell proliferation, cycle and migration. More keratinocyte wound healing and migration was shown in the experimental group (1.22 μg/mL. AAPE treatment significantly stimulated stress fiber formation, which was linked to the RhoA-ROCK pathway. We identified 48 protein spots in 2-D gel analysis and selected proteins were divided into 64% collagen components and 30% non-collagen components as shown by the MALDI-TOF analysis. Antibody array results contained growth factor/cytokine such as HGF, FGF-1, G-CSF, GM-CSF, IL-6, VEGF, and TGF-β3 differing from that shown by 2-D analysis. Conclusion: AAPE activates HK proliferation and migration. These results highlight the potential of the topical application of AAPE in the treatment of skin regeneration.

  2. Pathophysiological role of the acute inflammatory response during acetaminophen hepatotoxicity

    Neutrophils are recruited into the liver after acetaminophen (AAP) overdose but the pathophysiological relevance of this acute inflammatory response remains unclear. To address this question, we compared the time course of liver injury, hepatic neutrophil accumulation and inflammatory gene mRNA expression for up to 24 h after treatment with 300 mg/kg AAP in C3Heb/FeJ and C57BL/6 mice. Although there was no relevant difference in liver injury (assessed by the increase of plasma alanine aminotransferase activities and the areas of necrosis), the number of neutrophils and the expression of several pro-inflammatory genes (e.g., tumor necrosis factor-α, interleukin-1β and macrophage inflammatory protein-2) was higher in C3Heb/FeJ than in C57BL/6 mice. In contrast, the expression of the anti-inflammatory genes interleukin-10 and heme oxygenase-1 was higher in C57BL/6 mice. Despite substantial hepatic neutrophil accumulation, none of the liver sections from both strains stained positive for hypochlorite-modified proteins, a specific marker for a neutrophil-induced oxidant stress. In addition, treatment with the NADPH oxidase inhibitors diphenyleneiodonium chloride or apocynin or the anti-neutrophil antibody Gr-1 did not protect against AAP hepatotoxicity. Furthermore, although intercellular adhesion molecule-1 (ICAM-1) was previously shown to be important for neutrophil extravasation and tissue injury in several models, ICAM-1-deficient mice were not protected against AAP-mediated liver injury. Together, these data do not support the hypothesis that neutrophils aggravate liver injury induced by AAP overdose

  3. Secondhand smoke in combination with ambient air pollution exposure is associated with increasedx CpG methylation and decreased expression of IFN-γ in T effector cells and Foxp3 in T regulatory cells in children

    Kohli Arunima

    2012-09-01

    Full Text Available Abstract Background Secondhand smoke (SHS and ambient air pollution (AAP exposures have been associated with increased prevalence and severity of asthma and DNA modifications of immune cells. In the current study, we examined the association between SHS and AAP with DNA methylation and expression of interferon-gamma (IFN-γ and forkhead box protein 3 (Foxp3 in T cell populations. Methods Subjects 7–18 years old were recruited from Fresno (high AAP; n = 62 and Stanford, CA (low AAP; n = 40 and divided into SHS-exposed (Fresno: n = 31, Stanford: n = 6 and non-SHS-exposed (nSHS; Fresno: n = 31, Stanford: n = 34 groups. T cells purified from peripheral blood were assessed for levels of DNA methylation and expression of IFN-γ (in effector T cells or Foxp3 (in regulatory T cells. Results Analysis showed a significant increase in mean % CpG methylation of IFN-γ and Foxp3 associated with SHS exposure (IFN-γ: FSHS 62.10%, FnSHS 41.29%, p p Foxp3: FSHS 74.60%, FnSHS 54.44%, p p IFN-γ: FSHS 0.75, FnSHS 1.52, p p Foxp3: FSHS 0.75, FnSHS 3.29, p p IFN-γ: FSHS vs. SSHS, p p Foxp3: FSHS vs. SSHS, p p IFN-γ: FSHS vs. SSHS, p p Foxp3: FSHS vs. SSHS, p p IFN-γ: p = 0.15; Foxp3: p = 0.27, nor was Foxp3 expression (p = 0.08; IFN-γ expression was significantly decreased in AAP-only subjects (p  Conclusions Exposures to SHS and AAP are associated with significant hypermethylation and decreased expression of IFN-γ in Teffs and Foxp3 in Tregs. Relative contributions of each exposure to DNA modification and asthma pathogenesis warrant further investigation.

  4. Au@Ag/Au nanoparticles assembled with activatable aptamer probes as smart ``nano-doctors'' for image-guided cancer thermotherapy

    Shi, Hui; Ye, Xiaosheng; He, Xiaoxiao; Wang, Kemin; Cui, Wensi; He, Dinggeng; Li, Duo; Jia, Xuekun

    2014-07-01

    Although nanomaterial-based theranostics have increased positive expectations from cancer treatment, it remains challenging to develop in vivo ``nano-doctors'' that provide high-contrast image-guided site-specific therapy. Here we designed an activatable theranostic nanoprobe (ATNP) via self-assembly of activatable aptamer probes (AAPs) on Au@Ag/Au nanoparticles (NPs). As both quenchers and heaters, novel Au@Ag/Au NPs were prepared, showing excellent fluorescence quenching and more effective near-infrared photothermal therapy than Au nanorods. The AAP comprised a thiolated aptamer and a fluorophore-labeled complementary DNA; thus, the ATNP with quenched fluorescence in the free state could realize signal activation through target binding-induced conformational change of the AAP, and then achieve on-demand treatment under image-guided irradiation. By using S6 aptamer as the model, in vitro and in vivo studies of A549 lung cancer verified that the ATNP greatly improved imaging contrast and specific destruction, suggesting a robust and versatile theranostic strategy for personalized medicine in future.Although nanomaterial-based theranostics have increased positive expectations from cancer treatment, it remains challenging to develop in vivo ``nano-doctors'' that provide high-contrast image-guided site-specific therapy. Here we designed an activatable theranostic nanoprobe (ATNP) via self-assembly of activatable aptamer probes (AAPs) on Au@Ag/Au nanoparticles (NPs). As both quenchers and heaters, novel Au@Ag/Au NPs were prepared, showing excellent fluorescence quenching and more effective near-infrared photothermal therapy than Au nanorods. The AAP comprised a thiolated aptamer and a fluorophore-labeled complementary DNA; thus, the ATNP with quenched fluorescence in the free state could realize signal activation through target binding-induced conformational change of the AAP, and then achieve on-demand treatment under image-guided irradiation. By using S6 aptamer as

  5. Adverse Effects and Toxicity of the Atypical Antipsychotics: What is Important for the Pediatric Emergency Medicine Practitioner

    Rasimas, J.J.; Liebelt, Erica L.

    2012-01-01

    Medications are being used with greater frequency to address pediatric mental health problems, and in recent years atypical antipsychotic (AAP) prescriptions have increased more than any other class. Acute care practitioners must be aware of the pharmacology of AAPs and the conditions, on- and off-label, for which they are prescribed. This involves identifying and managing side effects that manifest both mentally and physically. Although “atypicality” confers a lower risk of movement side effects compared to conventional agents, children are more sensitive than adults to extrapyramidal reactions. Like adults, they also may present with toxic sedation, confusion, cardiovascular dysfunction, and metabolic derangements. Evaluation and management of these toxicities requires an index of suspicion, a careful symptom and medication history, physical examination, and targeted interventions. This review is designed to orient the emergency practitioner to the challenging task of recognizing and treating adverse effects related to acute and chronic atypical antipsychotic exposure in children. PMID:23471213

  6. Asymmetric dimethylarginine in somatically healthy schizophrenia patients treated with atypical antipsychotics

    Jørgensen, Anders; Knorr, Ulla Benedichte Søsted; Soendergaard, Mia Greisen;

    2015-01-01

    BACKGROUND: Schizophrenia is associated with increased cardiovascular morbidity and mortality. Asymmetric dimethylarginine (ADMA), an endogenous inhibitor of the nitric oxide synthase, and the L-arginine:ADMA ratio are markers of endothelial dysfunction that predict mortality and adverse outcome in...... a range of cardiovascular disorders. Increased ADMA levels may also lead to increased oxidative stress. We hypothesized that ADMA and the L-arginine:ADMA ratio are increased in somatically healthy schizophrenia patients treated with atypical antipsychotics (AAP), and that the ADMA and the L......-arginine: ADMA ratio are positively correlated to measures of oxidative stress. METHODS: We included 40 schizophrenia patients treated with AAP, but without somatic disease or drug abuse, and 40 healthy controls. Plasma concentrations of ADMA and L-arginine were determined by high-performance liquid...

  7. Surveying Turkish high school and university student attitudes and approaches to physics problem solving

    Balta, Nuri; Singh, Chandralekha

    2016-01-01

    Student attitudes and approaches to problem solving can impact how well they learn physics. Prior research in the US using a validated Attitude and Approaches to Problem Solving (AAPS) survey suggests that there are major differences between students in introductory physics and astronomy courses and physics experts in terms of their attitudes and approaches to physics problem solving. Here we discuss the validation, administration and analysis of data for the Turkish version of the AAPS survey for high school and university students in Turkey. After the validation and administration of the Turkish version of the survey, the analysis of the data was conducted by grouping the data by grade level, school type, and gender. While there are no statistically significant differences between the averages of various groups on the survey, overall, the university students in Turkey were more expert-like than vocational high school students. On an item by item basis, there are statistically differences between the average...

  8. Surveying college introductory physics students' attitudes and approaches to problem solving

    Mason, Andrew

    2016-01-01

    Students' attitudes and approaches to problem solving in physics can greatly impact their actual problem solving practices and also influence their motivation to learn and ultimately the development of expertise. We developed and validated an attitudes and approaches to problem solving (AAPS) survey and administered it to students in the introductory physics courses in a typical large research university in the US. Here, we discuss the development and validation of the survey and analysis of the student responses to the survey questions in introductory physics courses. The introductory physics students' responses to the survey questions were also compared with those of physics faculty members and physics PhD students. We find that introductory students are in general less expert-like than the physics faculty members and PhD students. Moreover, on some AAPS survey questions, the responses of students and faculty have unexpected trends. Those trends were interpreted via in

  9. Cadmium-induced oxidative stress in potato tuber

    Andrzej Stroiński

    2014-02-01

    Full Text Available Short-term treatment of tuber discs of potato (Solanum tuberosum L. with cadmium chloride elevated the concentration of active oxygen species (.O-2, H202 and activated the antioxidative system. Two cultivars, Bintje and Bzura, susceptible and tolerant, respectively, to cadmium were examined. In more tolerant, control tissues the activity of ascorbic acid peroxidase (AAP and catalase (CAT was higher than in the sensitive ones. During first hours of stress, the inhibition of superoxide dismutase (SOD, CAT and AAP was observed and it comes from inactivation of enzymes by cadmium ions. A subsequent activity increase of the enzymes aroused earlier in tolerant tissues. It seems therefore, that tolerant tissues possess a more efficient antioxidative system.

  10. Anorexia and Attachment: Dysregulated Defense and Pathological Mourning

    elisa edelvecchio

    2014-10-01

    Full Text Available The role of Defensive exclusion (Deactivation and Segregated Systems in the development of early relationships and related to subsequent manifestations of symptoms of eating disorders was assessed using the Adult Attachment Projective Picture System (AAP. Fifty-one DSM-IV diagnosed women with anorexia participated in the study. Anorexic patients were primarily classified as dismissing or unresolved. Quantitative and qualitative analyses of defensive exclusion were carried out. Results showed potential benefits of using the AAP defense exclusion coding system, in addition to the main attachment classifications, in order to better understand the developmental issues involved in anorexia. Discussion concerned the processes, such as pathological mourning, that may underlie the associations between dismissing and unresolved attachment and anorexia. Implications for developmental research and clinical nosology are discussed.

  11. Quantitative Enzymatic and Immunologic Histophotometry of Diseased Human Kid-Ney Tissues Using Tv-Camera and Computer Assisted Image Processing Systems.

    Heinert, G.; Mondorf, W.

    1982-11-01

    High speed image processing was used to analyse morphologic and metabolic characteristics of clinically relevant kidney tissue alterations.Qualitative computer-assisted histophotometry was performed to measure alterations in levels of the enzymes alkaline phosphatase (Ap),alanine aminopeptidase (AAP),g-glutamyltranspepti-dase (GGTP) and A-glucuronidase (B-G1) and AAP and GGTP immunologically determined in prepared renal and cancer tissue sections. A "Mioro-Videomat 2" image analysis system with a "Tessovar" macroscope,a computer-assisted "Axiomat" photomicroscope and an "Interactive Image Analysis System (IBAS)" were employed for analysing changes in enzyme activities determined by changes in absorbance or transmission.Diseased kidney as well as renal neoplastic tissues could be distinguished by significantly (wilcoxon test,phuman kidney tissues.This image analysis techniques might be of potential use in diagnostic and prognostic evaluation of renal cancer and diseased kidney tissues.

  12. From porous gold nanocups to porous nanospheres and solid particles - A new synthetic approach

    Ihsan, Ayesha

    2015-05-01

    We report a versatile approach for the synthesis of porous gold nanocups, porous gold nanospheres and solid gold nanoparticles. Gold nanocups are formed by the slow reduction of gold salt (HAuCl4{dot operator}3H2O) using aminoantipyrene (AAP) as a reducing agent. Adding polyvinylpyrrolidone (PVP) to the gold salt followed by reduction with AAP resulted in the formation of porous gold nanospheres. Microwave irradiation of both of these porous gold particles resulted in the formation of slightly smaller but solid gold particles. All these nanoparticles are thoroughly characterized by UV-visible spectroscopy, scanning electron microscopy (SEM), high resolution transmission electron microscopy (HR-TEM) and bright-field tomography. Due to the larger size, porous nature, low density and higher surface area, these nanomaterials may have interesting applications in catalysis, drug delivery, phototherapy and sensing.

  13. Esiplaanil heli / Harri Slip

    Slip, Harri

    2015-01-01

    Kõrvaklapid hinnaga alla 200 €: AKG Y50BT, Audio-Technica, Beyerdynamic Custom Street, Bose SoundTrue around-ear II, Creative Aurvana Gold, Denon AH-MM200, Focal Spirit One S, Grado SR 80e, JBL Everest V300, Klipsch Reference On Ear, Panasonic RP-HD10, Philips SHB8850NC, Sennheiser HD 25, SMS Audio On-Ear Wired Sport, Sony MDR-100AAP

  14. Use of cough and cold preparations during breastfeeding.

    Mitchell, J L

    1999-12-01

    Adverse reactions in infants from maternal drug ingestion depend largely on the amount of milk consumed by the infant, timing of breastfeeding in relation to dosing, dose of the medication, dosing interval, and duration of therapy. When taking medications, breastfeeding mothers should be instructed to take their medication after breastfeeding, at the lowest effective dose and for the shortest duration. Overall, there are few data from human studies on the use of antihistamines, decongestants, and cough products during breastfeeding. Studies of pseudoephedrine, triprolidine, and loratadine in humans conclude that low levels of each drug would reach a breastfed infant. Since triprolidine and pseudoephedrine are also considered compatible with breastfeeding by the AAP, these 2 drugs should be the first-line choices. Codeine is considered compatible with breastfeeding by the AAP, and would be an acceptable choice for short-term use as a cough suppressant. It is important to note that many of the liquid cough and cold products contain alcohol. In addition, many of the combination products are a mixture of an antihistamine and a decongestant and may also contain aspirin, acetaminophen, ibuprofen, or caffeine. It is preferable for nursing mothers to only take medications that are necessary and to avoid such combination products. The AAP considers alcohol, acetaminophen, ibuprofen, and caffeine compatible with breastfeeding. Aspirin has been associated with significant negative effects on some nursing infants, and the AAP recommends giving aspirin to nursing mothers with caution. Mothers taking cough and cold products should watch for adverse events in their breastfed infants. Infants may experience paradoxical central nervous stimulation from antihistamines and irritability and insomnia from decongestants. PMID:10776186

  15. NCBI nr-aa BLAST: CBRC-FRUB-02-0117 [SEVENS

    Full Text Available CBRC-FRUB-02-0117 ref|NP_001075589.1| blood-brain barrier large neutral amino acid ...mall subunit 1 (L-type amino acid transporter 1) (4F2 light chain) (4F2 LC) (4F2LC) (LAT1 light chain) gb|AAP47189.1| blood-brain bar...rier large neutral amino acid transporter light chain [Oryctolagus cuniculus] NP_001075589.1 7e-70 61% ...

  16. Investigation of Antioxidant and Antimicrobial Potential of Chloroform and Petroleum Ether Extracts of Selected Medicinal Plants of Bangladesh

    Zulfiker, A. H. M.; P P Roy; Momin, M. A. M.; Khan, M.S.; Bulbul, I. J.; T. Ahmed; Rana, M. S.

    2013-01-01

    Aim: To investigate the antioxidant and antimicrobial potential of Chloroform and Pet ether extracts of Manilkara zapota (MZCE, MZPE), Polyalthia longifolia (PLCE, PLPE), Abroma augusta (AACE, AAPE) Ficus hispida (FHCE, FHPE), Vitex negundo (VNCE, VNPE) plants. Study Design: In vitro antioxidant and antimicrobial study. Place and Duration of Study: Department of Pharmacy, School of Science & Engineering, Southeast University, Banani, Dhaka between June 2011 and March 2012. Methodology: In...

  17. Aortic Arch Atherosclerosis in Ischaemic Stroke of Unknown Origin Affects Prognosis

    Abe, Arata; Harada-Abe, Mina; Ueda, Masayuki; Katano, Takehiro; Nakajima, Masataka; Muraga, Kanako; Suda, Satoshi; Nishiyama, Yasuhiro; Okubo, Seiji; Mishina, Masahiro; Katsura, Ken-ichiro; Katayama, Yasuo

    2014-01-01

    Background Cerebral infarction of unknown origin at admission accounts for half of all cerebral infarction cases in some institutions. However, the factors associated with cerebral infarction prognosis have not been sufficiently examined. Here, we investigated whether aortic arch plaques (AAPs) on transoesophageal echocardiography (TOE) were associated with the prognosis of cerebral infarction of unknown origin at admission. Methods Of 571 patients who were hospitalised between June 2009 and ...

  18. Impact of Utilizing Pharmacy Students as Workforce for Hawai‘i Asthma Friendly Pharmacy Project

    Ma, Carolyn S; Nett, Blythe; Kishaba, Gregg; Gomez, Lara

    2015-01-01

    A partnership was formed between the University of Hawai‘i at Hilo Daniel K. Inouye College of Pharmacy (DKICP) and the Department of Health to carry out the Hawai‘i Asthma Friendly Pharmacy Project (HAFPP), which utilizes pharmacy students as a workforce to administer Asthma Control Tests™ (ACT), and provide Asthma Action Plans (AAP) and inhaler technique education. Evaluation of data from a pilot project in 2008 with first and second year students prompted more intensive training in therape...

  19. Droplet Microfluidic Technique for the Study of Fermentation

    Krzysztof Churski; Artur Ruszczak; Slawomir Jakiela; Piotr Garstecki

    2015-01-01

    We demonstrate a technique that uses microdroplets for culturing and selecting bacterial cultures in a model biotechnological application. We propose an assay for determination of ethanol concentration that provides increased dynamic range and is compatible with droplet microfluidic screening technologies. The assay comprises two enzymes—alcohol oxidase (AOX) and horseradish peroxidase (HRP)—and a colorimetric readout system of phenol-4-sulfonic acid (PSA) and 4-aminoantipyrine (4-AAP). The m...

  20. Historical Overview of Transitional Feeding Recommendations and Vegetable Feeding Practices for Infants and Young Children

    Kleinman, Ronald E.; Coletta, Frances A.

    2016-01-01

    Although recommendations for introducing solid foods to infants and young children have changed significantly since the beginning of the 20th century, vegetable consumption recommendations have always been an important part of the child-feeding repertoire. In 1958, the first report of the American Academy of Pediatrics (AAP) Committee on Nutrition stated that developmental maturity of the gut and neuromuscular system, growth rate, and activity level were good indicators for determining when t...

  1. Enteroaggregative Escherichia coli O78:H10, the cause of an outbreak of urinary tract infection

    Olesen, Bente; Scheutz, Flemming; Andersen, Rebecca L; Menard, Megan; Boisen, Nadia; Johnston, Brian; Hansen, Dennis S; Krogfelt, Karen; Nataro, James P; Johnson, James R

    2012-01-01

    by molecular methods as enteroaggregative E. coli (EAEC) (P <0.001). Genes present in >90% of outbreak isolates included fimH (type 1 fimbriae; ubiquitous in E. coli); fyuA, traT, and iutA (associated with extraintestinal pathogenic E. coli [ExPEC]); and sat, pic, aatA, aggR, aggA, ORF61, aaiC, aap...

  2. AcEST: DK962271 [AcEST

    Full Text Available TST39A01NGRL0013_G17 641 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0013_G17. 5' end seq ... 810 Query: 460 VVPTGGIPQPKEKLSPLGTIAAAPWGSALILPISYSYI CMMGSQGLTDASKLAILNANYM 639 V+PTGGIP+P E+ SPLGTI+AAP ... WGSALILPISY+YI ... MMGS GLTDASK+AILNANYM Sbjct: 811 VIPTGGIPEP-EQTSPL ... GTISAAPWGSALILPISYTYI AMMGSGGLTDASKIAILNANYM 869 Score = 38.1 bits (87), ... 822 Query: 460 VVPTGGIPQPKEKLSPLGTIAAAPWGSALILPISYSYI CMMGSQGLTDASKLAILNANYM 639 VVPTGGIP P E PLG+I+AAPWG ...

  3. AcEST: DK958768 [AcEST

    Full Text Available TST39A01NGRL0002_O02 684 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0002_O02. 5' end seq ... IGVKKHLAPFLPSHPVVPTGGIPQPKEKLSPLGTIAAAPWGSALILPISYSYI CMMGSQG 181 IGVK+HLAPFLPSHPV+PTGGIP+P E+ SPLGTI+AAP ... WGSALILPISY+YI ... MMGS G Sbjct: 796 IGVKQHLAPFLPSHPVIPTGGIPEP-EQTSPL ... GTISAAPWGSALILPISYTYI AMMGSGG 854 Query: 182 LTDASKLAILNANYMAKRLENHYPVLFR ... IGVKKHLAPFLPSHPVVPTGGIPQPKEKLSPLGTIAAAPWGSALILPISYSYI CMMGSQG 181 IGVKKHLAP+LPSHPVVPTGGIP P +K PLG I+AAPW ...

  4. Chapter Oral Health Advocates: A Nationwide Model for Pediatrician Peer Education and Advocacy about Oral Health

    Lewis, Charlotte W.; Lauren Barone; Rocio B. Quinonez; Suzanne Boulter; Mouradian, Wendy E.

    2013-01-01

    Objective. (1) To describe an innovative program training US pediatricians to be Chapter Oral Health Advocates (COHAs). (2) To provide insight into COHAs’ experiences disseminating oral health knowledge to fellow pediatricians. Patients and Methods. Interviews with 40 COHAs who responded to an email request, from a total of 64 (62% response). Transcripts were analyzed for common themes about COHA activities, facilitators, and barriers. Results. COHAs reported positive experiences at the AAP ...

  5. The cauliflower mosaic virus (CaMV) 35S promoter sequence alters the level and patterns of activity of adjacent tissue- and organ-specific gene promoters.

    Zheng, Xuelian; Deng, Wei; Luo, Keming; Duan, Hui; Chen, Yongqin; McAvoy, Richard; Song, Shuiqing; Pei, Yan; Li, Yi

    2007-08-01

    Here we report the effect of the 35S promoter sequence on activities of the tissue- and organ-specific gene promoters in tobacco plants. In the absence of the 35S promoter sequence the AAP2 promoter is active only in vascular tissues as indicated by expression of the AAP2:GUS gene. With the 35S promoter sequence in the same T-plasmid, transgenic plants exhibit twofold to fivefold increase in AAP2 promoter activity and the promoter becomes active in all tissue types. Transgenic plants hosting the ovary-specific AGL5:iaaM gene (iaaM coding an auxin biosynthetic gene) showed a wild-type phenotype except production of seedless fruits, whereas plants hosting the AGL5:iaaM gene along with the 35S promoter sequence showed drastic morphological alterations. RT-PCR analysis confirms that the phenotype was caused by activation of the AGL5:iaaM gene in non-ovary organs including roots, stems and flowers. When the pollen-, ovule- and early embryo-specific PAB5:barnase gene (barnase coding a RNase gene) was transformed, the presence of 35S promoter sequence drastically reduced transformation efficiencies. However, the transformation efficiencies were restored in the absence of 35S promoter, indicating that the 35S promoter might activate the expression of PAB5:barnase in non-reproductive organs such as calli and shoot primordia. Furthermore, if the 35S promoter sequence was replaced with the NOS promoter sequence, no alteration in AAP2, AGL5 or PAB5 promoter activities was observed. Our results demonstrate that the 35S promoter sequence can convert an adjacent tissue- and organ-specific gene promoter into a globally active promoter. PMID:17340093

  6. Modeling the Potential Impact of the 2014 American Academy of Pediatrics Respiratory Syncytial Virus Prophylaxis Guidance on Preterm Infant RSV Outcomes

    McLaurin, Kimmie K.; Chatterjee, Archana; Makari, Doris

    2015-01-01

    Introduction The American Academy of Pediatrics (AAP) Committee on Infectious Diseases issued updated guidance on respiratory syncytial virus (RSV) prophylaxis in 2014. This report models the potential impact of the new guidance on RSV outcomes in preterm infants 29–34 weeks’ gestational age (wGA) without chronic lung disease in the United States. Methods The number of preterm infants was estimated using 2012 natality data. Palivizumab utilization prior to the 2014 guidance update was estimat...

  7. Distribution of free-living and particle-attached aerobic anoxygenic phototrophic bacteria in marine environments

    Lami, R.; Čuperová, Zuzana; Ras, J.; Lebaron, P.; Koblížek, Michal

    2009-01-01

    Roč. 55, č. 1 (2009), s. 31-38. ISSN 0948-3055 R&D Projects: GA ČR GA206/07/0241; GA AV ČR 1QS500200570 Institutional research plan: CEZ:AV0Z50200510 Keywords : AAP bacteria * photoheterotrophy * free-living bacteria Subject RIV: EE - Microbiology, Virology Impact factor: 1.743, year: 2009

  8. Meeting Report: Metabolites in Safety Testing (MIST) Symposium—Safety Assessment of Human Metabolites: What’s REALLY Necessary to Ascertain Exposure Coverage in Safety Tests?

    GAO Hongying; Jacobs, Abigail; White, Ronald E.; Booth, Brian P.; Obach, R Scott

    2013-01-01

    In the 2012 AAPS metabolites in safety testing (MIST) symposium held in Chicago, IL, USA, on October 15, 2012, regulatory experts and industrial scientists joined together to discuss their perspectives and strategies in addressing contemporary MIST recommendations (FDA 2008, International Conference on Harmonization (ICH) M3(R2), ICH M(R2) Q&A). Overall, these regulatory guidances indicate that metabolites identified in human plasma should circulate at similar or greater concentrations in at ...

  9. The myADHDportal.com Improvement Program: An innovative quality improvement intervention for improving the quality of ADHD care among community-based pediatricians

    Epstein, Jeffery N.; Langberg, Joshua M.; Lichtenstein, Philip K.; Kolb, Rebecca; Simon, John O

    2013-01-01

    Though the American Academy of Pediatrics has developed and disseminated clear evidence-based guidelines for ADHD care, community-based pediatricians often have difficulty implementing these guidelines. New strategies are needed to improve the quality of care received by children with ADHD and to improve utilization of the AAP consensus guidelines by pediatricians. An evidence-based quality improvement intervention has been developed that effectively improves the quality of ADHD care delivere...

  10. Gene : CBRC-OCUN-01-1579 [SEVENS

    Full Text Available CBRC-OCUN-01-1579 pseudo Novel UN B Odorant/olfactory and gustatory rec eptors O51A7_HUMAN 1e-48 ... 52% gb|AAP71064.1| olfactory rec eptor Olfr575 [Mus musculus] 5e-54 59% gnl|UG|Ocu#S ... mRNA for protein phosphatase-1 catalytic subunit (EC ... 3.1.3.-) /cds=p(241,1176) /gb=Y00701 /gi=1676 /ug= ...

  11. EST Table: FY746730 [KAIKOcDNA[Archive

    Full Text Available FY746730 E_FL_famL_31G04_F_0 11/11/04 99 %/310 aa gb|ACN86367.1| troponin T transcript variant B ... 44 %/181 aa F53A9.10b#CE34878#WBGene00006587#locus:tnt - 2#status:Confirmed#UniProt:Q7Z071#protein_id:AAP6 ...

  12. EST Table: FY761643 [KAIKOcDNA[Archive

    Full Text Available FY761643 E_FL_famL_42G23_R_0 11/11/04 99 %/280 aa gb|ACN86367.1| troponin T transcript variant B ... 44 %/181 aa F53A9.10b#CE34878#WBGene00006587#locus:tnt - 2#status:Confirmed#UniProt:Q7Z071#protein_id:AAP6 ...

  13. EST Table: FS775378 [KAIKOcDNA[Archive

    Full Text Available FS775378 E_FL_fcaL_07N14_R_0 10/09/28 98 %/163 aa gb|ACN86367.1| troponin T transcript variant B ... 50 %/121 aa F53A9.10b#CE34878#WBGene00006587#locus:tnt - 2#status:Confirmed#UniProt:Q7Z071#protein_id:AAP6 ...

  14. EST Table: FS778531 [KAIKOcDNA[Archive

    Full Text Available FS778531 E_FL_fcaL_17P07_R_0 10/09/28 97 %/167 aa gb|ACN86367.1| troponin T transcript variant B ... 51 %/123 aa F53A9.10b#CE34878#WBGene00006587#locus:tnt - 2#status:Confirmed#UniProt:Q7Z071#protein_id:AAP6 ...

  15. One-step in situ synthesis of polyamide microcapsules with inorganic payload and their transformation into responsive thermoplastic composite materials

    Dencheva, Nadya Vasileva; Denchev, Z.; Lanceros-Méndez, S.; Ezquerra Sanz, T.

    2016-01-01

    Well-dispersed loads of finely powdered metals, metal oxides, several carbon allotropes or nanoclays are incorporated into highly porous polyamide 6 microcapsules in controllable amounts via an original one-step in situ fabrication technique. It is based on activated anionic polymerization (AAP) of ε-caprolactam in a hydrocarbon solvent performed in the presence of the respective micro- or nanosized loads. The forming microcapsules with typical diameters of 25-50 µm entrap up ...

  16. Barriers to Pediatricians’ Adherence to American Academy of Pediatrics Oral Health Referral Guidelines: North Carolina General Dentists’ Opinions

    Long, C. Marshall; Quinonez, Rocio B.; Rozier, R. Gary; Kranz, Ashley M.; Lee, Jessica Y.

    2015-01-01

    Purpose The purposes of this study were to: (1) assess knowledge, attitudes, and behaviors of North Carolina general dentists (GDs) regarding American Academy of Pediatrics (AAP) dental referral guidelines; and (2) determine factors that influence pediatricians’ ability to comply with AAP guidelines. Methods One thousand GDs were surveyed to determine barriers toward acceptance of physician referrals of infants and toddlers. The primary outcome using ordered logistic regression was GDs’ acceptance of children described in five case scenarios, with different levels of risk and oral health status. Results GDs believed pediatricians should refer patients at risk for caries to a dentist. While 61 to 75 percent of GDs were willing to accept low caries risk referrals of infants and toddlers, only 35 percent would accept referrals when caries was present. Predictors of referral acceptance were correct knowledge about AAP guidelines (OR=2.0, 95%CI=1.2-3.3), confidence in pro- viding preventive care to infants and toddlers (OR=2.6, 95%CI=1.3-4.9), and agreement that parents see importance in dental referrals (OR=2.1, 95% CI=1.2-3.6). Conclusions This study identified factors influencing acceptance of pediatrician referrals for the age one dental visit among North Carolina GDs and highlighted challenges pediatricians face in referring young children for dental care. PMID:25197996

  17. SIDS and other sleep-related infant deaths: expansion of recommendations for a safe infant sleeping environment.

    Moon, Rachel Y

    2011-11-01

    Despite a major decrease in the incidence of sudden infant death syndrome (SIDS) since the American Academy of Pediatrics (AAP) released its recommendation in 1992 that infants be placed for sleep in a nonprone position, this decline has plateaued in recent years. Concurrently, other causes of sudden unexpected infant death that occur during sleep (sleep-related deaths), including suffocation, asphyxia, and entrapment, and ill-defined or unspecified causes of death have increased in incidence, particularly since the AAP published its last statement on SIDS in 2005. It has become increasingly important to address these other causes of sleep-related infant death. Many of the modifiable and nonmodifiable risk factors for SIDS and suffocation are strikingly similar. The AAP, therefore, is expanding its recommendations from focusing only on SIDS to focusing on a safe sleep environment that can reduce the risk of all sleep-related infant deaths, including SIDS. The recommendations described in this policy statement include supine positioning, use of a firm sleep surface, breastfeeding, room-sharing without bed-sharing, routine immunizations, consideration of using a pacifier, and avoidance of soft bedding, overheating, and exposure to tobacco smoke, alcohol, and illicit drugs. The rationale for these recommendations is discussed in detail in the accompanying "Technical Report--SIDS and Other Sleep-Related Infant Deaths: Expansion of Recommendations for a Safe Infant Sleeping Environment," which is included in this issue of Pediatrics (www.pediatrics.org/cgi/content/full/128/5/e1341). PMID:22007004

  18. Promoting psychosocial adaptation of youths in residential care through animal-assisted psychotherapy.

    Balluerka, Nekane; Muela, Alexander; Amiano, Nora; Caldentey, Miguel A

    2015-12-01

    The goal of this study was to examine the influence of animal-assisted psychotherapy (AAP) on the psychosocial adaptation of a group of adolescents in residential care who had suffered traumatic childhood experiences and who presented with mental health problems. This study recruited 63 youths (mean age=15.27, SD=1.63) who were divided into two groups: a treatment group of 39 youths (19 female and 20 male; mean age=15.03, SD=0.51) and a control group of 24 (five female and 19 male; mean age=15.67, SD=1.63). The youths who underwent the AAP program had higher school adjustment in comparison to their peers who did not receive treatment. Their hyperactive behavior decreased, and they showed better social skills, more leadership, and fewer attention problems. They also showed a more positive attitude toward their teachers in comparison to controls. No differences were observed in other variables associated with clinical symptoms or personal adjustment. These results suggest that AAP can be effective with teenagers who have suffered childhood traumas and who present with problems of psychosocial adaptation. PMID:26443670

  19. Serial Ultrasound Monitoring for Early Recognition of Asparaginase Associated Pancreatitis in Children With Acute Lymphoblastic Leukemia

    Raja, Raheel Altaf; Schmiegelow, K.; Henriksen, Birthe Merete;

    2015-01-01

    BACKGROUND: Acute lymphoblastic leukemia (ALL) is the most common cancer in children and L-asparaginase is an essential component of the treatment. Cessation of L-asparaginase decreases event free survival. Acute pancreatitis is the toxicity that most commonly results in cessation of L-asparagina......BACKGROUND: Acute lymphoblastic leukemia (ALL) is the most common cancer in children and L-asparaginase is an essential component of the treatment. Cessation of L-asparaginase decreases event free survival. Acute pancreatitis is the toxicity that most commonly results in cessation of L...... protocol, with PEG-asparaginase of 2 or 6 week intervals, for 30 weeks had their pancreas monitored using serial ultrasound in order to detect early signs of inflammation. RESULTS: Nineteen of 31 eligible patients were included. Three of the included patients developed AAP. None of the patients, including...... the three patients that developed AAP, had signs of inflammatory edema or pancreas enzymes above three times the upper normal limit prior to AAP. CONCLUSION: We found no signs of inflammatory edema within the pancreas on ultrasound during treatment with PEG-asparginase in our cohort prior to...

  20. Health information technology and the medical home.

    2011-05-01

    The American Academy of Pediatrics (AAP) supports development and universal implementation of a comprehensive electronic infrastructure to support pediatric information functions of the medical home. These functions include (1) timely and continuous management and tracking of health data and services over a patient's lifetime for all providers, patients, families, and guardians, (2) comprehensive organization and secure transfer of health data during patient-care transitions between providers, institutions, and practices, (3) establishment and maintenance of central coordination of a patient's health information among multiple repositories (including personal health records and information exchanges), (4) translation of evidence into actionable clinical decision support, and (5) reuse of archived clinical data for continuous quality improvement. The AAP supports universal, secure, and vendor-neutral portability of health information for all patients contained within the medical home across all care settings (ambulatory practices, inpatient settings, emergency departments, pharmacies, consultants, support service providers, and therapists) for multiple purposes including direct care, personal health records, public health, and registries. The AAP also supports financial incentives that promote the development of information tools that meet the needs of pediatric workflows and that appropriately recognize the added value of medical homes to pediatric care. PMID:21518710

  1. Medical Versus Nonmedical Immunization Exemptions for Child Care and School Attendance.

    2016-09-01

    Routine childhood immunizations against infectious diseases are an integral part of our public health infrastructure. They provide direct protection to the immunized individual and indirect protection to children and adults unable to be immunized via the effect of community immunity. All 50 states, the District of Columbia, and Puerto Rico have regulations requiring proof of immunization for child care and school attendance as a public health strategy to protect children in these settings and to secondarily serve as a mechanism to promote timely immunization of children by their caregivers. Although all states and the District of Columbia have mechanisms to exempt school attendees from specific immunization requirements for medical reasons, the majority also have a heterogeneous collection of regulations and laws that allow nonmedical exemptions from childhood immunizations otherwise required for child care and school attendance. The American Academy of Pediatrics (AAP) supports regulations and laws requiring certification of immunization to attend child care and school as a sound means of providing a safe environment for attendees and employees of these settings. The AAP also supports medically indicated exemptions to specific immunizations as determined for each individual child. The AAP views nonmedical exemptions to school-required immunizations as inappropriate for individual, public health, and ethical reasons and advocates for their elimination. PMID:27573087

  2. Novel parvoviruses in reptiles and genome sequence of a lizard parvovirus shed light on Dependoparvovirus genus evolution.

    Pénzes, Judit J; Pham, Hanh T; Benkö, Mária; Tijssen, Peter

    2015-09-01

    Here, we report the detection and partial genome characterization of two novel reptilian parvoviruses derived from a short-tailed pygmy chameleon (Rampholeon brevicaudatus) and a corn snake (Pantherophis guttatus) along with the complete genome analysis of the first lizard parvovirus, obtained from four bearded dragons (Pogona vitticeps). Both homology searches and phylogenetic tree reconstructions demonstrated that all are members of the genus Dependoparvovirus. Even though most dependoparvoviruses replicate efficiently only in co-infections with large DNA viruses, no such agents could be detected in one of the bearded dragon samples, hence the possibility of autonomous replication was explored. The alternative ORF encoding the full assembly activating protein (AAP), typical for the genus, could be obtained from reptilian parvoviruses for the first time, with a structure that appears to be more ancient than that of avian and mammalian parvoviruses. All three viruses were found to harbour short introns as previously observed for snake adeno-associated virus, shorter than that of any non-reptilian dependoparvovirus. According to the phylogenetic calculations based on full non-structural protein (Rep) and AAP sequences, the monophyletic cluster of reptilian parvoviruses seems to be the most basal out of all lineages of genus Dependoparvovirus. The suspected ability for autonomous replication, results of phylogenetic tree reconstruction, intron lengths and the structure of the AAP suggested that a single Squamata origin instead of the earlier assumed diapsid (common avian-reptilian) origin is more likely for the genus Dependoparvovirus of the family Parvoviridae. PMID:26067293

  3. Oxytocin enhances the experience of attachment security

    Buchheim, Anna; Heinrichs, Markus; George, Carol; Pokorny, Dan; Koops, Eva; Henningsen, Peter; O’Connor, Mary-Frances; Gundel, Harald

    2011-01-01

    Summary Repeated interactions between infant and caregiver result in either secure or insecure relationship attachment patterns, and insecure attachment may affect individual emotion-regulation and health. Given that oxytocin enhances social approach behavior in animals and humans, we hypothesized that oxytocin might also promote the experience of attachment security in humans. Within a 3-week interval 26 healthy male students classified with an insecure attachment pattern were invited twice to an experimental session. Within each session, a single dose of oxytocin or placebo was administered, using a double-blind, placebo-controlled within-subject design. In both conditions, subjects completed an attachment task based on the Adult Attachment Projective Picture System (AAP). Thirty-two AAP picture system presentations depicted attachment-related events (e.g. illness, solitude, separation, loss), and were each accompanied by four prototypical phrases representing one secure and three insecure attachment categories. In the oxytocin condition, a significant proportion of these insecure subjects (N = 18; 69%) changed their rankings of “secure attachment” phrases towards the more appropriate for the AAP picture presentation, and the same subjects decreased in overall rating of the “insecure attachment” phrases. In particular, there was a significant decrease in the number of subjects ranking the pictures with “insecure-preoccupied” phrases from the placebo to the oxytocin condition. We find that a single dose of intranasally administered oxytocin is sufficient to induce a significant increase in the experience of attachment security in adults classified previously as insecure. PMID:19457618

  4. Experience in diagnosis and treatment of asparaginase-associated pancreatitis in children%门冬酰胺酶相关胰腺炎的诊治体会

    陈再生; 李健

    2014-01-01

    Objective To analyze the clinical characteristics and the course of diagnosis and therapy of PEG-asparaginase associated pancreatitis (AAP)in childhood,and to reveal the pathophysiology of AAP,enhance the ability of diagnosis and treament.Method Data of 13 cases with AAP in childhood seen from March 2011 to March 2014 were analyzed with regard to clinical manifestations,laboratory findings,imaging feature and treatment.Result AAP was found in 12 of acute lymphoblastic leukemia (ALL) and 1 of non-Hodgkin's lymphoma (NHL),8 were boys and 5 were girls,with a mean age 6 years.In 12 cases AAP occurred during the induction-remission treatment,in 1 case during the maintenance-intensification phase.AAP occurred after a median of two doses,and 9 d (median) from the latest administration of PEG-asparaginase.The major manifestations of AAP was abdominal pain (11/13).At the time of AAP diagnosis during the first 48 hours the median peak serum amylase and serum lipase levels were 559 U/L (range 118 -1 585,upper normal limit:125) and 934 U/L (range 221-1 673,upper normal limit:300).Three cases with serum amylase and serum lipase levels above 3 times upper normal limit were repeatedly complicated with pancreatic pseudocyst; 11 patients had abnormal CT imaging,8 cases revealed effusion around the pancreas,and 4 cases had pseudocyst.Therapy with ulinastatin,octreotide acetate,glucocorticoid could relieve abdominal pain significantly.Three cases underwent abdominal puncture drainage and 5 cases fulfilled nasojejunal nutrition therapy.Nine of them were cured,4 developed pseudocyst,in 2 AAP vanished gradually and 2 died with pseudocyst.Conclusion The major manifestations of AAP were abdominal pain,but sometimes apparent and sometimes latent.Condition of acute pancreatitis may exacerbate rapidly and easily.Early identification had significance.Pancreatic pseudocyst suggested a poor prognosis.%目的 分析门冬酰胺酶相关胰腺炎(AAP)的临床特征和诊治经过.方法 收集

  5. Acquisition, Replication and Inoculation of Candidatus Liberibacter asiaticus following Various Acquisition Periods on Huanglongbing-Infected Citrus by Nymphs and Adults of the Asian Citrus Psyllid.

    El-Desouky Ammar

    Full Text Available The Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae, is the primary vector of Candidatus Liberibacter asiaticus (Las implicated as causative agent of citrus huanglongbing (citrus greening, currently the most serious citrus disease worldwide. Las is transmitted by D. citri in a persistent-circulative manner, but the question of replication of this bacterium in its psyllid vector has not been resolved. Thus, we studied the effects of the acquisition access period (AAP by nymphs and adults of D. citri on Las acquisition, multiplication and inoculation/transmission. D. citri nymphs or adults (previously non-exposed to Las were caged on Las-infected citrus plants for an AAP of 1, 7 or 14 days. These 'Las-exposed' psyllids were then transferred weekly to healthy citrus or orange jasmine plants, and sampled via quantitative polymerase chain reaction (qPCR analysis 1-42 days post-first access to diseased plants (padp; all tested nymphs became adults 7-14 days padp. Our results indicate that following 1 or 7 day AAP as nymphs 49-59% of Las-exposed psyllids became Las-infected (qPCR-positive, whereas only 8-29% of the psyllids were infected following 1-14 day AAP as adults. Q-PCR analysis also indicated that Las titer in the Las-exposed psyllids (relative to that of the psyllid S20 ribosomal protein gene was: 1 significantly higher, and increasing at a faster rate, following Las acquisition as nymphs compared to that following Las acquisition as adults; 2 higher as post-acquisition time of psyllids on healthy plants increased reaching a peak at 14-28 days padp for nymphs and 21-35 days padp for adults, with Las titer decreasing or fluctuating after that; 3 higher with longer AAP on infected plants, especially with acquisition as adults. Our results strongly suggest that Las multiplies in both nymphs and adults of D. citri but attains much higher levels in a shorter period of time post-acquisition when acquired by nymphs than when acquired by

  6. Precipitation estimation in mountainous terrain using multivariate geostatistics. Part I: structural analysis

    Hevesi, Joseph A.; Istok, Jonathan D.; Flint, Alan L.

    1992-01-01

    Values of average annual precipitation (AAP) are desired for hydrologic studies within a watershed containing Yucca Mountain, Nevada, a potential site for a high-level nuclear-waste repository. Reliable values of AAP are not yet available for most areas within this watershed because of a sparsity of precipitation measurements and the need to obtain measurements over a sufficient length of time. To estimate AAP over the entire watershed, historical precipitation data and station elevations were obtained from a network of 62 stations in southern Nevada and southeastern California. Multivariate geostatistics (cokriging) was selected as an estimation method because of a significant (p = 0.05) correlation of r = .75 between the natural log of AAP and station elevation. A sample direct variogram for the transformed variable, TAAP = ln [(AAP) 1000], was fitted with an isotropic, spherical model defined by a small nugget value of 5000, a range of 190 000 ft, and a sill value equal to the sample variance of 163 151. Elevations for 1531 additional locations were obtained from topographic maps to improve the accuracy of cokriged estimates. A sample direct variogram for elevation was fitted with an isotropic model consisting of a nugget value of 5500 and three nested transition structures: a Gaussian structure with a range of 61 000 ft, a spherical structure with a range of 70 000 ft, and a quasi-stationary, linear structure. The use of an isotropic, stationary model for elevation was considered valid within a sliding-neighborhood radius of 120 000 ft. The problem of fitting a positive-definite, nonlinear model of coregionalization to an inconsistent sample cross variogram for TAAP and elevation was solved by a modified use of the Cauchy-Schwarz inequality. A selected cross-variogram model consisted of two nested structures: a Gaussian structure with a range of 61 000 ft and a spherical structure with a range of 190 000 ft. Cross validation was used for model selection and for

  7. Ambient air pollution associated with suppressed serologic responses to Pneumocystis jirovecii in a prospective cohort of HIV-infected patients with Pneumocystis pneumonia.

    Robert J Blount

    Full Text Available Ambient air pollution (AAP may be associated with increased risk for Pneumocystis pneumonia (PCP. The mechanisms underlying this association remain uncertain.To determine if real-life exposures to AAP are associated with suppressed IgM antibody responses to P. jirovecii in HIV-infected (HIV+ patients with active PCP, and to determine if AAP, mediated by suppressed serologic responses to Pneumocystis, is associated with adverse clinical outcomes.We conducted a prospective cohort study in HIV+ patients residing in San Francisco and admitted to San Francisco General Hospital with microscopically confirmed PCP. Our AAP predictors were ambient air concentrations of particulate matter of < 10 µm in diameter (PM10 and < 2.5 µm in diameter (PM2.5, nitrogen dioxide (NO2, ozone (O3, and sulfur dioxide (SO2 measured immediately prior to hospital admission and 2 weeks prior to admission. Our primary outcomes were the IgM serologic responses to four recombinant P. jirovecii major surface glycoprotein (Msg constructs: MsgC1, MsgC3, MsgC8, and MsgC9.Elevated PM10 and NO2 exposures immediately prior to and two weeks prior to hospital admission were associated with decreased IgM antibody responses to P. jirovecii Msg. For exposures immediately prior to admission, every 10 µg/m(3 increase in PM10 was associated with a 25 to 35% decrease in IgM responses to Msg (statistically significant for all the Msg constructs, and every 10 ppb increase in NO2 was associated with a 19-45% decrease in IgM responses to Msg (statistically significant for MsgC8 and MsgC9. Similar findings were seen with exposures two weeks prior to admission, but for fewer of the Msg constructs.Real life exposures to PM10 and NO2 were associated with suppressed IgM responses to P. jirovecii Msg in HIV+ patients admitted with PCP, suggesting a mechanism of immunotoxicity by which AAP increases host susceptibility to pulmonary infection.

  8. Korean Medication Algorithm for Bipolar Disorder 2014: comparisons with other treatment guidelines

    Jeong JH

    2015-06-01

    Full Text Available Jong-Hyun Jeong,1 Jeong Goo Lee,2,3 Moon-Doo Kim,4 Inki Sohn,5 Se-Hoon Shim,6 Hee Ryung Wang,1 Young Sup Woo,1 Duk-In Jon,7 Jeong Seok Seo,8 Young-Chul Shin,9 Kyung Joon Min,10 Bo-Hyun Yoon,11 Won-Myong Bahk1 1Department of Psychiatry, College of Medicine, The Catholic University of Korea, Seoul, 2Department of Psychiatry, Haeundae Paik Hospital, College of Medicine, Paik Institute for Clinical Research, Inje University, 3Department of Health Science and Technology, Graduate School of Inje University, Busan, 4Department of Psychiatry, Jeju National University Hospital, Jeju, 5Department of Psychiatry, Keyo Hospital, Keyo Medical Foundation, Uiwang, 6Department of Psychiatry, Soonchunhyang University Cheonan Hospital, College of Medicine, Soonchunhyang University, Cheonan, 7Department of Psychiatry, Sacred Heart Hospital, College of Medicine, Hallym University, Anyang, 8Department of Psychiatry, Konkuk University Chungju Hospital, School of Medicine, Konkuk University, Chungju, 9Department of Psychiatry, Kangbuk Samsung Hospital, School of Medicine, Sungkyunkwan University, Seoul, 10Department of Psychiatry, Chung-Ang University Hospital, College of Medicine, Chung-Ang University, Seoul, 11Department of Psychiatry, Naju National Hospital, Naju, Korea Abstract: Our goal was to compare the recommendations of the Korean Medication Algorithm Project for Bipolar Disorder 2014 (KMAP-BP 2014 with other recently published guidelines for the treatment of bipolar disorder. We reviewed a total of four recently published global treatment guidelines and compared each treatment recommendation of the KMAP-BP 2014 with those in other guidelines. For the initial treatment of mania, there were no significant differences across treatment guidelines. All recommended mood stabilizer (MS or atypical antipsychotic (AAP monotherapy or the combination of an MS with an AAP as a first-line treatment strategy for mania. However, the KMAP-BP 2014 did not prefer monotherapy

  9. Recent and Past Musical Activity Predicts Cognitive Aging Variability: Direct Comparison with Leisure Activities

    Brenda eHanna-Pladdy

    2012-07-01

    Full Text Available Studies evaluating the impact of modifiable lifestyle factors on cognition offer potential insights into sources of cognitive aging variability. Recently, we reported an association between extent of musical instrumental practice throughout the life span (greater than 10 years on preserved cognitive functioning in advanced age . These findings raise the question of whether there are training-induced brain changes in musicians that can transfer to nonmusical cognitive abilities to allow for compensation of age-related cognitive declines. However, because of the relationship between engagement in lifestyle activities and preserved cognition, it remains unclear whether these findings are specifically driven by musical training or the types of individuals likely to engage in greater activities in general. The current study examined the type of leisure activity (musical versus other as well as the timing of engagement (age of acquisition, past versus recent in predictive models of successful cognitive aging. Seventy age and education matched older musicians (> 10 years and nonmusicians (ages 59-80 were evaluated on neuropsychological tests and life-style activities (AAP. Partition analyses were conducted on significant cognitive measures to explain performance variance in musicians. Musicians scored higher on tests of phonemic fluency, verbal immediate recall, judgment of line orientation (JLO, and Letter Number Sequencing (LNS, but not the AAP. The first partition analysis revealed education best predicted JLO in musicians, followed by recent musical engagement which offset low education. In the second partition analysis, early age of musical acquisition (< 9 years predicted enhanced LNS in musicians, while analyses for AAP, verbal recall and fluency were not predictive. Recent and past musical activity, but not leisure activity, predicted variability across verbal and visuospatial domains in aging. Early musical acquisition predicted auditory

  10. The acylaminoacyl peptidase from Aeropyrum pernix K1 thought to be an exopeptidase displays endopeptidase activity.

    Kiss, András L; Hornung, Balázs; Rádi, Krisztina; Gengeliczki, Zsolt; Sztáray, Bálint; Juhász, Tünde; Szeltner, Zoltán; Harmat, Veronika; Polgár, László

    2007-04-27

    Mammalian acylaminoacyl peptidase, a member of the prolyl oligopeptidase family of serine peptidases, is an exopeptidase, which removes acylated amino acid residues from the N terminus of oligopeptides. We have investigated the kinetics and inhibitor binding of the orthologous acylaminoacyl peptidase from the thermophile Aeropyrum pernix K1 (ApAAP). Complex pH-rate profiles were found with charged substrates, indicating a strong electrostatic effect in the surroundings of the active site. Unexpectedly, we have found that oligopeptides can be hydrolysed beyond the N-terminal peptide bond, demonstrating that ApAAP exhibits endopeptidase activity. It was thought that the enzyme is specific for hydrophobic amino acids, in particular phenylalanine, in accord with the non-polar S1 subsite of ApAAP. However, cleavage after an Ala residue contradicted this notion and demonstrated that P1 residues of different nature may bind to the S1 subsite depending on the remaining peptide residues. The crystal structures of the complexes formed between the enzyme and product-like inhibitors identified the oxyanion-binding site unambiguously and demonstrated that the phenylalanine ring of the P1 peptide residue assumes a position different from that established in a previous study, using 4-nitrophenylphosphate. We have found that the substrate-binding site extends beyond the S2 subsite, being capable of binding peptides with a longer N terminus. The S2 subsite displays a non-polar character, which is unique among the enzymes of this family. The S3 site was identified as a hydrophobic region that does not form hydrogen bonds with the inhibitor P3 residue. The enzyme-inhibitor complexes revealed that, upon ligand-binding, the S1 subsite undergoes significant conformational changes, demonstrating the plasticity of the specificity site. PMID:17350041

  11. Effects of Matrix Composition on Detection Threshold Estimates for Methyl Anthranilate and 2-Aminoacetophenone

    Demetra M. Perry

    2016-05-01

    Full Text Available Conceptually, a detection threshold represents the lowest concentration at which an individual or a group of individuals can reliably perceive a given stimulus, with a commonly used operational definition of 50% performance above chance. Estimated detection thresholds (DTs, however, are often reported in the literature with little attention given to the matrix in which the stimuli were evaluated. Here, we highlight the influence of matrix effects on DTs for two odor-active compounds commonly found in Vitis Labrusca wines. Differences in orthonasal DTs for methyl anthranilate (MA and 2-aminoacetophenone (2AAP in water, a model wine system, and wine were demonstrated using a within-subject design and forced choice (i.e., criterion free psychophysical methods. Six sample triads, each containing two blanks and one spiked sample, were presented to participants with the instructions to choose the “different” sample, and this was repeated in different matrices (water, model wine, and wine. The estimated DTs for both compounds were significantly lower in water versus the model wine system and wine. This finding recapitulates the strong need to carefully consider the nature of the delivery matrix when determining and comparing threshold estimates across studies. Additionally, data from prior reports have suggested DTs for MA and 2AAP may differ by two orders of magnitude in spite of their structural similarity. We failed to confirm this difference here: although 2AAP thresholds were somewhat lower than MA thresholds, differences were much smaller than what had been suggested previously. This, again, emphasizes the need to make comparisons within the same individuals, using appropriate methods with sufficient numbers of participants.

  12. Apodized-aperture pixel design to increase high-frequency DQE and reduce noise aliasing in x-ray detectors

    Ismailova, Elina; Karim, Karim; Cunningham, Ian A.

    2015-03-01

    The detective quantum efficiency (DQE) of an x-ray detector, expressed as a function of spatial frequency, describes the ability to produce high-quality images relative to an ideal detector. While the DQE normally decreases substantially with increasing frequency, we describe an approach that can be used to improve the DQE response by increasing the DQE at high spatial frequencies. The approach makes use of an apodized-aperture pixel (AAP) design that requires use of a high-resolution x-ray converter such as selenium coupled to a sensor array with very small physical sensor elements, such as CMOS sensors. While sensors with elements of 10 - 25 μm are too small for most practical applications in medical radiography, we describe how larger image pixels of a practical size can be synthesized to provide a better DQE than simple binning or using physical pixels of the same size. A theoretical cascaded-systems analysis shows the DQE at the image sampling cut-off frequency can be improved by up to a factor of 2.5x. The AAP approach was validated experimentally using a CMOS/CsI-based detector having 0.05-mm sensor elements. Using AAP images with 0.2-mm pixels, the high-frequency DQE value was increased from 0.2 to 0.4 compared to simple 4x4 binning. It is concluded that ultra-high-resolution sensors can be used to optimize the high-frequency performance of x-ray detectors and make substantial improvements in image quality for visualization of small stuctures and fine image detail in comparison to current imaging systems.

  13. Promoting the well-being of children whose parents are gay or lesbian.

    2013-04-01

    To promote optimal health and well-being of all children, the American Academy of Pediatrics (AAP) supports access for all children to (1) civil marriage rights for their parents and (2) willing and capable foster and adoptive parents, regardless of the parents' sexual orientation. The AAP has always been an advocate for, and has developed policies to support, the optimal physical, mental, and social health and well-being of all infants, children, adolescents, and young adults. In so doing, the AAP has supported families in all their diversity, because the family has always been the basic social unit in which children develop the supporting and nurturing relationships with adults that they need to thrive. Children may be born to, adopted by, or cared for temporarily by married couples, nonmarried couples, single parents, grandparents, or legal guardians, and any of these may be heterosexual, gay or lesbian, or of another orientation. Children need secure and enduring relationships with committed and nurturing adults to enhance their life experiences for optimal social-emotional and cognitive development. Scientific evidence affirms that children have similar developmental and emotional needs and receive similar parenting whether they are raised by parents of the same or different genders. If a child has 2 living and capable parents who choose to create a permanent bond by way of civil marriage, it is in the best interests of their child(ren) that legal and social institutions allow and support them to do so, irrespective of their sexual orientation. If 2 parents are not available to the child, adoption or foster parenting remain acceptable options to provide a loving home for a child and should be available without regard to the sexual orientation of the parent(s). PMID:23519941

  14. Primary care providers' knowledge, practices, and perceived barriers to the treatment and prevention of childhood obesity.

    Spivack, Jordan G; Swietlik, Maggie; Alessandrini, Evaline; Faith, Myles S

    2010-07-01

    This study evaluated primary care providers' (PCPs, pediatricians, and nurse practitioners) knowledge, current practices, and perceived barriers to childhood obesity prevention and treatment, with an emphasis on first-year well-child care visits. A questionnaire was distributed to 192 PCPs in the primary care network at The Children's Hospital of Philadelphia (CHOP) addressing (i) knowledge of obesity and American Academy of Pediatrics (AAP) guidelines, (ii) anticipatory guidance practices at well visits regarding nutrition and exercise, and (iii) perceived barriers to childhood obesity treatment and prevention. Eighty pediatricians and seven nurse practitioners responded, and a minority correctly identified the definition (26%) and prevalence (9%) of childhood overweight and AAP guidelines for exercise (39%) and juice consumption (44%). Most PCPs (81%) spent 11-20 min per well visit during the first 2 years, and 79% discussed diet, nutrition, and exercise for > or =3 min. Although >95% of PCPs discussed juice, fruits and vegetables, sippy cups, and finger foods during the first year, over 35% never discussed fast food, TV, or candy, and 55% never discussed exercise. Few rated current resources as adequate to treat or prevent childhood obesity. Over 90% rated the following barriers for obesity prevention and treatment as important or very important: parent is not motivated, child is not motivated, parents are overweight, families often have fast food, watch too much TV, and do not get enough exercise. In conclusion, there is much room to improve PCPs' knowledge of obesity and AAP guidelines. Although PCPs rate fast-food consumption, TV viewing, and lack of exercise as important treatment barriers, many never discussed these topics during the first year. PMID:19910934

  15. Detección de genes de virulencia del patotipo enteroagregativo en cepas de Escherichia coli aisladas de fuentes de agua subterránea de la provincia del Chaco, Argentina

    Liliana S Lösch

    2015-06-01

    Full Text Available En la provincia del Chaco, el agua subterránea representa una fuente alternativa, y muchas veces única, para el consumo humano; esta es utilizada en el 14 % de los hogares. A pesar de que se reconoce el riesgo de la exposición al agua contaminada, la prevalencia de los diferentes patotipos de Escherichia coli en ambientes acuáticos no ha sido bien caracterizada. E. coli enteroagregativo (ECEA es un patógeno emergente cuya importancia en la salud pública mundial se incrementó y quedó claramente establecida en los últimos años. El objetivo del presente trabajo fue detectar la presencia de ECEA típico mediante el reconocimiento de los factores de virulencia aap, AA probe y aggR por reacción en cadena de la polimerasa, en fuentes de agua subterráneas de la provincia del Chaco. Se identificó E. coli en 36 (38,7 % de las 93 muestras estudiadas, provenientes de diferentes localidades. De esos 36 aislamientos, se identificaron 6 (16,7 % portadores de los genes de ECEA, lo que representa una prevalencia del 6,4 % considerando las 93 fuentes de agua subterránea estudiadas. De esos 6 aislamientos, 3 eran portadores del gen aap, 2 del gen AA probe y uno de la combinación aggR/aap. El presente trabajo representa el primer aporte en el estudio de la presencia y distribución de genes de virulencia de ECEA en fuentes de agua subterránea de la región.

  16. [Diagnostic problems and prognostic factors in prostate cancer].

    Tarján, Miklós

    2016-03-01

    We aimed to refine the methodology for discriminating ductal (DAP) and acinar adenocarcinomas (AAP) of the prostate preoperatively with a high degree of accuracy, and confirm that prostate carcinoma of ductal origin is a more aggressive subtype. Moreover, we intended to evaluate the clinical utility of transrectal ultrasound-guided systematic sextant or octant biopsies in prediction of extracapsular extension (ECE) at radical prostatectomy. A blinded retrospective analysis of 3-dimensional histology specimens from 110 consecutive radical prostatectomy (RP) cases operated between 2000 and 2006 was carried out (average follow-up: 5.1 years). The samples were also analyzed for 9 different biomarkers. We performed a retrospective analysis of 84 cases of patients who underwent transrectal ultrasound-guided systematic sextant (in 60 cases) or octant (in 24 cases) biopsy. The presence of ECE was correlated to the number of positive biopsies on each side of the prostate by chi-square analysis. Sensitivity, specificity, positive and negative predictive values were calculated for both positive (two or three positive biopsies per side) and negative (no or only one positive biopsy per side) test results. The number of positive cores was thereafter combined with two other parameters: prostate-specific antigen (PSA) and Gleason score. 3-dimensional and conventional histology classified 97 cases of AAP and 13 cases of DAP. DAP cases had a significantly greater frequency of pT3a and more advanced cancers (p20 mm tumor focus (p=0.0020), highgrade PIN (p=0.0079), Gleason score ≥7 (p10 ng/ml vs. ≤10 ng/ml and more than one positive cores vs. 0 or 1 positives) identified patients at high or low risk of ECE, respectively. On the extremes, with only lowrisk parameters none of the 10 patients, while 77% of those with high-risk group had ECE at RP. Both 3-dimensional histology and the three selected biomarkers can accurately distinguish DAP from AAP. This discriminatory ability offers

  17. Asparaginase-associated pancreatitis in children with acute lymphoblastic leukaemia in the NOPHO ALL2008 protocol

    Raja, Raheel A; Schmiegelow, Kjeld; Albertsen, BK;

    2014-01-01

    L-asparaginase is an important drug in the treatment of childhood acute lymphoblastic leukaemia (ALL). Treatment is associated with several toxicities, including acute pancreatitis. Clinical course, presentation, re-exposure to L-asparginase after pancreatitis and risk of recurrent pancreatitis...... within an asparaginase-intensive protocol has been poorly reported. Children (1-17 years) on the ongoing Nordic Society of Paediatric Haematology and Oncology (NOPHO) ALL2008 protocol with asparaginase-associated pancreatitis (AAP) diagnosed between 2008 and 2012 were identified through the online NOPHO...

  18. DOI简介

    2014-01-01

    <正>DOI(Digital Object Identifier),即文献数字对象识别号,为美国出版商协会(The Association of American Publishers,AAP)于1994年建立;1997年成为数字资源命名的一项标准。DOI能让学术文章永久寻址,实现文献永续存在。《厦门大学法律评论》从2013年起,通过台湾元照出版公司提供的平台,正式加入DOI

  19. Uus kuum Eesti disain / Silvia Pärmann, Maris Takk

    Pärmann, Silvia

    2015-01-01

    Karl Tauli disainitud taburet "Ämblu", Aap Piho puidust ümmargune laud, Marit Ilisoni tekkmantlid kollektsioonist "Longing for Sleep/Magada tahaks", Narma vaibakollektsioon "OPEN 2015/16" (disainerid Monika Järg, Kaidi Ploomipuu), Annike Laigo vaibakollektsioon "XX", Kairi Katmanni vaip "Storytellers", Valhalla Factory kollektsiooni "Daydreamers" ripptool, Raili Keivi betoonist ja portselanist nõudekollektsioon, Tarmo Luisu valgusti "Kassett", Stella Soomlaisi käekotid, Karin Kallase ja Erik Pasti (Stuudio Nahk) disainitud jalatsid, käekotid ja ehted, Kuula + Jylhä jalatsikollektsioon, Mokoko (disainer Mari Maripuu) nahast aksessuaarid

  20. Proceso de sensibilización y empoderamiento local frente al uso sostenible del suelo en la microcuenca El Limón, cuencas altas Sabana Yegua, República Dominicana

    Maldané Cuello Espinosa

    2011-01-01

    Este artículo trata el desarrollo de un proceso de sensibilización y empoderamiento por parte de los productores y productoras agrícolas de la microcuenca El Limón, dentro del complejo de cuencas que alimentan a la presa Sabana Yegua, en República Dominicana. La metodología implementada en el proceso se basa en un enfoque participativo local, que a su vez está basado en el modelo de Acción Ambiental Positiva (AAP), el cual incluye los elementos de habilidades y procedimientos, conceptos, empo...

  1. PDF for Healthcare and Child Health Data Forms.

    Zuckerman, Alan E; Schneider, Joseph H; Miller, Ken

    2008-01-01

    PDF-H is a new best practices standard that uses XFA forms and embedded JavaScript to combine PDF forms with XML data. Preliminary experience with AAP child health forms shows that the combination of PDF with XML is a more effective method to visualize familiar data on paper and the web than the traditional use of XML and XSLT. Both PDF-H and HL7 Clinical Document Architecture can co-exist using the same data for different display formats. PMID:18999257

  2. NCBI nr-aa BLAST: CBRC-PTRO-06-0019 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 0.0 99% ... ...CBRC-PTRO-06-0019 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  3. NCBI nr-aa BLAST: CBRC-TNIG-12-0002 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 2e-95 46% ... ...CBRC-TNIG-12-0002 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  4. NCBI nr-aa BLAST: CBRC-VPAC-01-1477 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 0.0 84% ... ...CBRC-VPAC-01-1477 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  5. NCBI nr-aa BLAST: CBRC-TBEL-01-2493 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 1e-116 90% ... ...CBRC-TBEL-01-2493 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  6. NCBI nr-aa BLAST: CBRC-CJAC-01-0928 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 0.0 91% ... ...CBRC-CJAC-01-0928 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  7. NCBI nr-aa BLAST: CBRC-STRI-01-2861 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 0.0 88% ... ...CBRC-STRI-01-2861 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  8. NCBI nr-aa BLAST: CBRC-MMUR-01-1599 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 0.0 89% ... ...CBRC-MMUR-01-1599 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  9. NCBI nr-aa BLAST: CBRC-FRUB-02-0029 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 1e-93 52% ... ...CBRC-FRUB-02-0029 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  10. NCBI nr-aa BLAST: CBRC-CFAM-03-0005 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 0.0 83% ... ...CBRC-CFAM-03-0005 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  11. NCBI nr-aa BLAST: CBRC-DNOV-01-2809 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 0.0 85% ... ...CBRC-DNOV-01-2809 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  12. NCBI nr-aa BLAST: CBRC-TTRU-01-0295 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 0.0 86% ... ...CBRC-TTRU-01-0295 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  13. NCBI nr-aa BLAST: CBRC-OLAT-09-0012 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 8e-93 50% ... ...CBRC-OLAT-09-0012 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  14. NCBI nr-aa BLAST: CBRC-HSAP-05-0018 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 0.0 99% ... ...CBRC-HSAP-05-0018 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  15. NCBI nr-aa BLAST: CBRC-ETEL-01-0940 [SEVENS

    Full Text Available CBRC-ETEL-01-0940 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  16. NCBI nr-aa BLAST: CBRC-HSAP-04-0027 [SEVENS

    Full Text Available CBRC-HSAP-04-0027 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  17. NCBI nr-aa BLAST: CBRC-OLAT-18-0070 [SEVENS

    Full Text Available CBRC-OLAT-18-0070 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  18. NCBI nr-aa BLAST: CBRC-TNIG-20-0000 [SEVENS

    Full Text Available CBRC-TNIG-20-0000 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  19. NCBI nr-aa BLAST: CBRC-FRUB-02-0257 [SEVENS

    Full Text Available CBRC-FRUB-02-0257 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  20. NCBI nr-aa BLAST: CBRC-CPOR-01-1990 [SEVENS

    Full Text Available CBRC-CPOR-01-1990 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  1. NCBI nr-aa BLAST: CBRC-CBRE-01-0701 [SEVENS

    Full Text Available CBRC-CBRE-01-0701 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  2. NCBI nr-aa BLAST: CBRC-TGUT-06-0015 [SEVENS

    Full Text Available CBRC-TGUT-06-0015 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  3. NCBI nr-aa BLAST: CBRC-ACAR-01-0438 [SEVENS

    Full Text Available CBRC-ACAR-01-0438 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  4. NCBI nr-aa BLAST: CBRC-CBRE-01-1053 [SEVENS

    Full Text Available CBRC-CBRE-01-1053 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  5. NCBI nr-aa BLAST: CBRC-XTRO-01-2768 [SEVENS

    Full Text Available CBRC-XTRO-01-2768 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  6. NCBI nr-aa BLAST: CBRC-PTRO-05-0018 [SEVENS

    Full Text Available CBRC-PTRO-05-0018 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  7. NCBI nr-aa BLAST: CBRC-DRER-07-0072 [SEVENS

    Full Text Available CBRC-DRER-07-0072 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  8. NCBI nr-aa BLAST: CBRC-MMUS-05-0020 [SEVENS

    Full Text Available CBRC-MMUS-05-0020 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  9. NCBI nr-aa BLAST: CBRC-BTAU-01-2281 [SEVENS

    Full Text Available CBRC-BTAU-01-2281 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  10. NCBI nr-aa BLAST: CBRC-PABE-05-0014 [SEVENS

    Full Text Available CBRC-PABE-05-0014 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  11. NCBI nr-aa BLAST: CBRC-ETEL-01-0465 [SEVENS

    Full Text Available CBRC-ETEL-01-0465 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  12. NCBI nr-aa BLAST: CBRC-DRER-01-0042 [SEVENS

    Full Text Available CBRC-DRER-01-0042 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  13. NCBI nr-aa BLAST: CBRC-RMAC-05-0007 [SEVENS

    Full Text Available CBRC-RMAC-05-0007 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  14. NCBI nr-aa BLAST: CBRC-CJAC-01-1653 [SEVENS

    Full Text Available CBRC-CJAC-01-1653 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  15. NCBI nr-aa BLAST: CBRC-GGAL-04-0036 [SEVENS

    Full Text Available CBRC-GGAL-04-0036 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  16. NCBI nr-aa BLAST: CBRC-GACU-16-0038 [SEVENS

    Full Text Available CBRC-GACU-16-0038 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  17. NCBI nr-aa BLAST: CBRC-LAFR-01-0624 [SEVENS

    Full Text Available CBRC-LAFR-01-0624 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  18. NCBI nr-aa BLAST: CBRC-CINT-01-0111 [SEVENS

    Full Text Available CBRC-CINT-01-0111 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  19. NCBI nr-aa BLAST: CBRC-CBRE-01-1028 [SEVENS

    Full Text Available CBRC-CBRE-01-1028 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  20. NCBI nr-aa BLAST: CBRC-ETEL-01-1373 [SEVENS

    Full Text Available CBRC-ETEL-01-1373 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  1. NCBI nr-aa BLAST: CBRC-OCUN-01-1620 [SEVENS

    Full Text Available CBRC-OCUN-01-1620 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  2. NCBI nr-aa BLAST: CBRC-FCAT-01-1015 [SEVENS

    Full Text Available CBRC-FCAT-01-1015 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  3. NCBI nr-aa BLAST: CBRC-CINT-01-0193 [SEVENS

    Full Text Available CBRC-CINT-01-0193 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  4. NCBI nr-aa BLAST: CBRC-CFAM-03-0027 [SEVENS

    Full Text Available CBRC-CFAM-03-0027 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  5. NCBI nr-aa BLAST: CBRC-GACU-07-0011 [SEVENS

    Full Text Available CBRC-GACU-07-0011 ref|NP_000721.1| cholecystokinin A receptor [Homo sapiens] sp|P32238|CCKAR_HUMAN Chole...cystokinin type A receptor (CCK-A receptor) (CCK-AR) (Cholecystokinin-1 receptor) (CCK1-R) gb|AAA35659.1| chole...cystokinin A receptor gb|AAA02819.1| cholecystokinin A receptor gb|AAA91123.1| chole...cystokinin type A receptor dbj|BAA90879.1| cholecystokinin type-A receptor [Homo ...sapiens] gb|AAP84362.1| cholecystokinin A receptor [Homo sapiens] gb|AAH74987.1| Cholecystokinin A receptor

  6. Study on preparation and antigenicity activity of glycosylation products derived from whey protein and auricularia auricula polysaccharide%黑木耳多糖-乳清蛋白复合物的制备及其抗原性的研究

    齐晓彦; 李春; 张微; 刘宁

    2012-01-01

    Protein and polysaccharide covalent complex through Maillard could form protein-polysaccharide conjugations which showed excellent properties.And the conjugates of whey protein and auricularia auricula polysaccharides(AAP)were studied by means of the dry-heating glycosylated reaction.It was shown that after the whey protein and Auricularia auricula polysaccharides(AAP)of different mass ratio reacting for different time,the antigenicity of β-LG and α-LA were estimated by indirect competition ELISA.The results indicated that the glycolsylation of whey protein could reduce the antigenicity of β-LG and α-LA.The optimum reaction condition were WPI and AAP(1:1 weight ratio)for 24 hours could reduce the antigenicity of whey protein effectively,The antigenicity of bovine milk β-LG and α-LA was reduced by conjugation with AAP,about 75.7% and 25% respectively.%蛋白质和多糖在控制条件下通过美拉德反应会发生一定程度的共价复合,能显示更优越的性能。采用黑木耳多糖作为糖基供体,用糖基化的手段与牛乳中乳清蛋白结合形成木耳多糖-乳清蛋白复合物,并在现有的条件下探索不同质量比与不同反应时间对糖基化进程的影响,采用间接竞争ELISA法测定复合物中β-乳球蛋白和α-乳白蛋白抗原性的影响。结果表明,乳清蛋白与黑木耳多糖质量比为1:1,反应时间为24h,是糖基化反应最佳条件并且能有效减低乳清蛋白抗原性,其中β-乳球蛋白抗原性降低率为75.7%,α-乳白蛋白抗原性降低率为25%。

  7. Dicty_cDB: CHI193 [Dicty_cDB

    Full Text Available 4237_196( AP004237 |pid:none) Proteus vulgaris plasmid Rts1 DN... 35 2.6 AJ698720_112( AJ698720 |pid:none) Yersinia pestis... ltkfqcirlylllkimnmsngnmh*itrisfsliiy*l*levsiqpmtvlnvrivh*ysh liyqifqnlisliqstmiqvtkldt*ksrvien*vieh*lvhvvvr...vqlmnihqlvlklv iv*ilekmenl Frame B: *fsfti*q***lfkmgnllshtygvpsiisiiiivsssifliilkkdkqkyinnqinnqi nnqivpeisnn...5 11 L36897 |L36897.1 Saccharomyces cerevisiae mitochondrion oxi3 gene, aapI gene, oli2 gene, ORF4, replication of origin (ori...dwytikgsknigrfrrnvqss*styy*y *pssnvfdctcy*k**i*ameicik*qgyhfh**sidcnwkfqsnl*qs*m*esyinihi *ftrysrisflsfnll*sk*qs*ilkslg**kikl*si

  8. Gene : CBRC-PVAM-01-0168 [SEVENS

    Full Text Available CBRC-PVAM-01-0168 UN B Odorant/olfactory and gustatory receptors OR6B1_HUMAN 1e-56 ...90% ref|NP_667275.1| olfactory receptor 449 [Mus musculus] gb|AAL60686.1| olfactory receptor MOR103-1 [Mus m...usculus] gb|AAP70959.1| olfactory receptor Olfr449 [Mus musculus] gb|EDL13462.1| olfactory receptor 449 [Mus... musculus] gb|AAI38167.1| Olfactory receptor 449 [Mus musculus] 5e-60 98% MSIAELV

  9. Gene : CBRC-CPOR-01-0842 [SEVENS

    Full Text Available CBRC-CPOR-01-0842 Novel UN B Odorant/olfactory and gustatory receptors OLF3_MOUSE 5...e-72 76% ref|NP_996786.1| olfactory receptor 3 [Mus musculus] sp|Q60879|OLF3_MOUSE Olfactory receptor 3 (Olfactory receptor... 136-14) (Odorant receptor K10) gb|AAL61134.1| olfactory receptor MOR136-14 [Mus musculus] gb|AAP70883.1| olfactor...y receptor Olfr3 [Mus musculus] emb|CAM16514.1| olfactory receptor... 3 [Mus musculus] gb|EDL08687.1| olfactory receptor 3 [Mus musculus] 6e-71 76% MRQSLRVKLVAMSWVIACACALL

  10. Technology assessment of RDX production

    Coburn, M.D. [New Mexico Inst. of Mining and Technology, Socorro, NM (United States)

    1995-04-01

    The known processes for producing RDX were assessed with the goal of identifying the process that would generate the least waste and pollution. It was concluded that the Bachman process employed at Holston AAP is the most economical process for producing RDX and that it probably produces less waste than any other process. It was generally agreed that the entire Holston operation is a very clean one that complies with all federal and state emission standards. In addition, a number of opportunities in which Holston could reduce their wastes were identified. Preliminary assessments of waste and pollution profiles for alternate materials, with emphasis on dual-use materials, were performed.

  11. Uus tootedisain = New product design / Monika Järg

    Järg, Monika, 1975-

    2012-01-01

    Tutvustatakse: mööblisari Smart (Tarmo Luisk, 2011) ja Slice (Tarmo Luisk, 2008), rattahoidja Typo (Tarmo Luisk) ja Tulip Fan Fan (Margus Triibmann, 2011), iste Seat (Monika Järg), mööbliseeria Maast: tool CHO1 ja laud TBO1 (Tõnis Kalve, Aap Piho, 2011), padjad KO! (Kärt Ojavee, 2011), vaibakollektsioon Unlimited (Kaidi Ploomipuu, Monika Järg, 2011), vann Aquator CLAUDE 170 (2011), büroomööbli seeria Metod (Iseasi, 2012), rippvalgusti Aero (Tõnis Vellama, 2011), tool Must ja nagi Z (Toivo Raidmets, 2008-2012)

  12. The Aminopeptidase Inhibitor CHR-2863 Is an Orally Bioavailable Inhibitor of Murine Malaria

    Skinner-Adams, Tina S.; Peatey, Christopher L.; Anderson, Karen; Trenholme, Katharine R.; Krige, David; Christopher L. Brown; Stack, Colin; Nsangou, Desire M. M.; Mathews, Rency T.; Thivierge, Karine; Dalton, John P; GARDINER, DONALD L.

    2012-01-01

    Malaria remains a significant risk in many areas of the world, with resistance to the current antimalarial pharmacopeia an ever-increasing problem. The M1 alanine aminopeptidase (PfM1AAP) and M17 leucine aminopeptidase (PfM17LAP) are believed to play a role in the terminal stages of digestion of host hemoglobin and thereby generate a pool of free amino acids that are essential for parasite growth and development. Here, we show that an orally bioavailable aminopeptidase inhibitor, CHR-2863, is...

  13. Safety and Efficacy Assessment of Flow Redistribution by Occlusion of Intrahepatic Vessels Prior to Radioembolization in the Treatment of Liver Tumors

    We evaluated the feasibility, safety, and efficacy of radioembolization (administered from one or two vascular points) after the redistribution of arterial blood flow in the liver in patients with hepatic neoplasms and arterial anatomic peculiarities (AAP). Twenty-four patients with liver neoplasms and AAP (graded according to Michel's classification) were included in the study. During pretreatment angiographic planning, all extrahepatic vessels that could feed the tumor were embolized and the intrahepatic vessels occluded in order to redistribute blood flow. The distribution of microspheres was initially assessed by using technetium-99m-labeled macroaggregated albumin (99mTc-MAA) from one of two vascular points before the administration of yttrium-90 (90Y)-radiolabeled resin microspheres. Perfusion of lesions situated in the redistributed segments (L-RS) and nonredistributed segments (L-NRS) were compared by assessing the distribution of 99mTc-MAA by SPECT/CT. Perfusion was graded as normal, reduced, or absent. 90Y resin microspheres were then injected from the same arterial sites as 99mTc-MAA and the tumor response recorded 3 months later. The tumor response in L-RS was compared with that in L-NRS and graded as better, similar, or worse. Among 11 patients with type I AAP in whom mainly vessels in segments I-III or IV were occluded, perfusion of L-RS was graded as similar (n = 7) or reduced (n = 4). Among the remaining 13 patients with AAP types III (n = 3), V (n = 4), VIII (n = 3), and others (n = 3) in which aberrant arteries were occluded, perfusion of L-RS was graded as similar (n = 9), reduced (n = 3), or absent (n = 1). Overall, 99mTc-MAA was present in the L-RS of 95.8% patients and the distribution of 99mTc-MAA in L-RS and L-NRS were graded as similar in 66.6% of patients. Compared with lesions in the L-NRS, tumor response in L-RS was similar in 23 cases and worse in 1 case. No complications were recorded after the administration of 90Y resin microspheres

  14. NCBI nr-aa BLAST: CBRC-OPRI-01-0352 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 1e-162 71% ... ...CBRC-OPRI-01-0352 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  15. NCBI nr-aa BLAST: CBRC-BTAU-01-1497 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 0.0 79% ... ...CBRC-BTAU-01-1497 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  16. NCBI nr-aa BLAST: CBRC-GGOR-01-0277 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 0.0 86% ... ...CBRC-GGOR-01-0277 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  17. NCBI nr-aa BLAST: CBRC-GACU-13-0014 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 1e-93 51% ... ...CBRC-GACU-13-0014 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  18. NCBI nr-aa BLAST: CBRC-ETEL-01-0080 [SEVENS

    Full Text Available gulation factor II (thrombin) receptor [Homo sapiens] gb|AAX32147.1| coagulation fa...ctor II receptor [synthetic construct] gb|ABM86025.1| coagulation factor II (thrombin) receptor [synthetic c...onstruct] gb|ABW03766.1| coagulation factor II (thrombin) receptor [synthetic construct] AAH02464.1 1e-166 75% ... ...CBRC-ETEL-01-0080 gb|AAH02464.1| Coagulation factor II (thrombin) receptor [Homo sapiens] gb|AAP35943.1| coa

  19. Gene : CBRC-MMUS-07-0515 [SEVENS

    Full Text Available CBRC-MMUS-07-0515 7 A Odorant/olfactory and gustatory receptors O52N5_HUMAN 1e-159 ...88% ref|NP_667254.1| olfactory receptor 669 [Mus musculus] gb|AAL60708.1| olfactory receptor MOR34-6 [Mus musculus...] gb|AAP71140.1| olfactory receptor Olfr669 [Mus musculus] gb|AAI04301.1| Olfactory receptor 669 [Mus musculus...] gb|EDL16746.1| olfactory receptor 669 [Mus musculus] 0.0 100% gnl|UG|Mm#S26988998 Mus musculus

  20. Gene : CBRC-MMUS-02-0242 [SEVENS

    Full Text Available CBRC-MMUS-02-0242 2 A Odorant/olfactory and gustatory receptors OR4P4_HUMAN 1e-117 ...66% ref|NP_667034.1| olfactory receptor 1184 [Mus musculus] gb|AAL60933.1| olfactory receptor MOR225-3 [Mus musculus...] gb|AAP71595.1| olfactory receptor Olfr1184 [Mus musculus] emb|CAM17508.1| olfactory receptor 1184 [Mus musculus...L27434.1| olfactory receptor 1184 [Mus musculus] 0.0 100% gnl|UG|Mm#S9745121 Mus musculus olfactory receptor

  1. Gene : CBRC-MMUS-10-0061 [SEVENS

    Full Text Available CBRC-MMUS-10-0061 10 A Odorant/olfactory and gustatory receptors OR7AH_HUMAN 1e-121... 71% ref|NP_667251.1| olfactory receptor 1351 [Mus musculus] gb|AAL60711.1| olfactory receptor MOR139-4 [Mus musculus...] gb|AAP71735.1| olfactory receptor Olfr1351 [Mus musculus] gb|AAI04113.1| Olfactory receptor 1351 [Mus musculus...] gb|EDL31709.1| mCG52449 [Mus musculus] 1e-180 100% gnl|UG|Mm#S26988969 Mus musculus

  2. Gene : CBRC-MMUS-11-0101 [SEVENS

    Full Text Available CBRC-MMUS-11-0101 11 A Odorant/olfactory and gustatory receptors OR3A4_HUMAN 1e-139... 83% gb|AAP70925.1| olfactory receptor Olfr399 [Mus musculus] emb|CAI24631.1| olfactory receptor 399 [Mus musculus...] gb|AAI20847.1| Olfactory receptor 399 [Mus musculus] gb|AAI20821.1| Olfactory receptor 399 [Mus musculus...] gb|EDL12753.1| olfactory receptor 399 [Mus musculus] 1e-177 100% gnl|UG|Mm#S34285683 Mus musculus

  3. Gene : CBRC-MMUS-11-0114 [SEVENS

    Full Text Available CBRC-MMUS-11-0114 11 A Odorant/olfactory and gustatory receptors OR3A1_HUMAN 1e-155... 84% ref|NP_666918.1| olfactory receptor 410 [Mus musculus] gb|AAL61051.1| olfactory receptor MOR255-5 [Mus musculus...] gb|AAP70932.1| olfactory receptor Olfr410 [Mus musculus] emb|CAI24527.1| olfactory receptor 410 [Mus musculus...] gb|EDL12768.1| mCG52814 [Mus musculus] 1e-180 100% gnl|UG|Mm#S9745013 Mus musculus

  4. Gene : CBRC-MMUS-07-0414 [SEVENS

    Full Text Available CBRC-MMUS-07-0414 7 A Odorant/olfactory and gustatory receptors O51A7_HUMAN 1e-133 ...76% ref|NP_667321.1| olfactory receptor 570 [Mus musculus] gb|AAL60639.1| olfactory receptor MOR8-3 [Mus musculus...] gb|AAP71060.1| olfactory receptor Olfr570 [Mus musculus] gb|AAI16967.1| Olfactory receptor 570 [Mus musculus...] gb|EDL16635.1| olfactory receptor 570 [Mus musculus] 1e-175 100% gnl|UG|Mm#S33850657 Mus mus

  5. Gene : CBRC-MMUS-09-0098 [SEVENS

    Full Text Available CBRC-MMUS-09-0098 9 A Odorant/olfactory and gustatory receptors OL147_MOUSE 1e-126 ...72% ref|NP_667015.1| olfactory receptor 905 [Mus musculus] gb|AAL60953.1| olfactory receptor MOR167-1 [Mus musculus...] gb|AAP71380.1| olfactory receptor Olfr905 [Mus musculus] gb|EDL25460.1| mCG53365 [Mus musculus] gb|...AAI50477.1| Olfactory receptor 905 [Mus musculus] 1e-173 98% gnl|UG|Mm#S39545474 Mus musculus

  6. Gene : CBRC-MMUS-11-0110 [SEVENS

    Full Text Available CBRC-MMUS-11-0110 pseudo Novel 11 A Odorant/olfactory and gustatory receptors OR1A1..._PANTR 2e-69 53% gb|AAP70929.1| olfactory receptor Olfr43 [Mus musculus] emb|CAI24518.1| olfactory receptor 43 [Mus musculus...] gb|AAI25465.1| Olfactory receptor 43 [Mus musculus] gb|AAI25469.1| Olfactory receptor 43 [Mus musculus...] gb|EDL12760.1| mCG1036248 [Mus musculus] 1e-81 61% gnl|UG|Mm#S35209285 Mus musculus

  7. Gene : CBRC-MMUS-19-0084 [SEVENS

    Full Text Available CBRC-MMUS-19-0084 19 A Odorant/olfactory and gustatory receptors OR9I1_HUMAN 1e-148... 82% ref|NP_667008.1| olfactory receptor 1502 [Mus musculus] gb|AAL60960.1| olfactory receptor MOR211-1 [Mus musculus...] gb|AAP71851.1| olfactory receptor Olfr1502 [Mus musculus] gb|AAI06811.1| Olfactory receptor 1502 [Mus musculus...] gb|EDL41523.1| mCG55155 [Mus musculus] 1e-180 100% gnl|UG|Mm#S27582255 Mus musculus

  8. Determination of Mercury (II Ion on Aryl Amide-Type Podand-Modified Glassy Carbon Electrode

    Sevgi Güney

    2011-01-01

    Full Text Available A new voltammetric sensor based on an aryl amide type podand, 1,8-bis(o-amidophenoxy-3,6-dioxaoctane, (AAP modified glassy carbon electrode, was described for the determination of trace level of mercury (II ion by cyclic voltammetry (CV and differential pulse voltammetry (DPV. A well-defined anodic peak corresponding to the oxidation of mercury on proposed electrode was obtained at 0.2 V versus Ag/AgCl reference electrode. The effect of experimental parameters on differential voltammetric peak currents was investigated in acetate buffer solution of pH 7.0 containing 1 × 10−1 mol L−1 NaCl. Mercury (II ion was preconcentrated at the modified electrode by forming complex with AAP under proper conditions and then reduced on the surface of the electrode. Interferences of Cu2+, Pb2+, Fe3+, Cd2+, and Zn2+ ions were also studied at two different concentration ratios with respect to mercury (II ions. The modified electrode was applied to the determination of mercury (II ions in seawater sample.

  9. Effects of polychlorinated biphenyls and lipemia on serum analytes

    Steinberg, K.K.; Freni-Titulaer, L.W.J.; Rogers, T.N.; Burse, V.W.; Mueller, P.W.; Stehr, P.A.; Miller, D.T.; Steele, G.

    1986-01-01

    Twelve serum analytes (triglycerides, cholesterol, total and conjugated bilirubin, high-density-lipoprotein cholesterol (HDL-C) alkaline phosphatase (AP), gammaglutamyl transferase (GGT), aspartate aminotransferase (AST), alanine aminotransferase (ALT), ..beta..-glucoronidase (..beta..-glu), alanine aminopeptidase (AAP), and 5'-nucleotidase (5'nuc)) were measured to investigate their correlation with exposure to polychlorinated biphenyls (PCBs) and 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT). The relationship between serum lipids, lipophilic toxicants, and the analytes was also evaluated. The ..beta..-glue, 5'nuc, triglycerides, cholesterol, and total bilirubin correlated positively and significantly with log concentrations of serum total PCBs and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE), a metabolite of DDT. The more highly chlorinated PCBs had significant, positive correlations with several serum analytes, but the less chlorinated PCBs correlated significantly and negatively only with HDL-cholesterol. Triglyercide- and cholesterol-rich lipoproteins were added to serum to determine the effects of lipids on these assays. Several were spuriously elevated. AP and ..beta..-glu were not affected by lipoprotein addition with the methods used in this study. AAP was increased significantly only at triglyceide concentrations exceeding 400 mg/dl.

  10. Biofilm characteristics of Staphylococcus epidermidis isolates associated with device-related meningitis.

    Stevens, Niall T

    2009-07-01

    Staphylococcus epidermidis biofilm causes device-related meningitis in neurosurgical patients. This study assessed the contribution of polysaccharide and protein to the development of a strong biofilm-positive phenotype in four S. epidermidis isolates associated with probable device-related meningitis, under varying environmental conditions. RT-PCR analysis of the intercellular adhesion operon (icaADBC) and assessment of polysaccharide intercellular adhesin (PIA) production indicated a correlation between increased icaA transcription and PIA production in ica(+) isolates grown in medium with 4 % ethanol and 4 % NaCl. Treatment of biofilm with sodium metaperiodate caused dispersion of adhered cells (P <0.0001), indicating involvement of PIA. Transcriptional levels of protein factors revealed that atlE transcription levels were similar in all isolates, whilst aap levels were variable, with induction being seen in two isolates following growth in the presence of alcohol or salt. Transcription of agr did not influence protein expression and RNAIII transcription varied among the strains. Although aap transcription was induced, the treatment of biofilm with proteinase K did not always disperse the biofilm. Our data suggest that, among the three ica(+) S. epidermidis isolates clinically associated with meningitis that were studied, PIA contributed to the strong biofilm-positive phenotype, whereas protein factors appeared to have a secondary role.

  11. Adult Attachment, Social Adjustment, and Well-Being in Drug-Addicted Inpatients.

    Delvecchio, Elisa; Di Riso, Daniela; Lis, Adriana; Salcuni, Silvia

    2016-04-01

    In recent years, attachment studies have gathered overwhelming evidence for a relation between insecure attachment and drug addiction. The existing literature predominantly addresses attachment styles and little attention is given to attachment-pattern-oriented studies. The current study explored how attachment, social adjustment, and well-being interact in 40 (28 men, 12 women; ages 20-52 years, M = 32.3, SD = 9.4) inpatients with drug addiction. The Adult Attachment Projective Picture System (AAP), the Social Adjustment Scale-Self-report (SAS-SR), and the General Health Questionnaire-28 (GHQ-28) were administered. Descriptive statistics were computed as well as differences between patterns of attachment in all variables were measured. None of the inpatients showed a secure attachment pattern: 7 scored as dismissing (18%), 5 preoccupied (12%) and 28 unresolved (70%). AAP stories were mainly connected with themes of danger, lack of protection, and helplessness. Inpatients classified as unresolved reported significantly higher maladjustment on the SAS-SR and GHQ-28 than those with resolved attachment patterns. Implications for clinicians and researchers are presented. PMID:27154381

  12. The activities of six exo-and endopeptidases in the substantia nigra, neostriatum, and cortex of the rat brain.

    Gallegos, M E; Zannatha, M M; Osornio, E G; Sánchez, A S; Posadas del rio, F A

    1999-12-01

    We determined the enzymatic activity and crude subcellular distribution of four exopeptidases: Dipeptidylaminopeptidase IV (DAP-IV), Alanyl aminopeptidase (AAP), Prolyl aminopeptidase (PAP) and gamma-Glutamyl transpeptidase (gammaGTP), and two endopeptidases: Postproline endopeptidase (PEP) and "Trypsin-like" peptidase ("T-L" P) in pars compacta (SNPC) and pars reticulata (SNPR) of substantia nigra, caudate-putamen (CAU) and cerebral cortex (CC) of the rat brain. We found: 1) DAP-IV activity is comparatively higher in SNPC and it is equally distributed in the postmitochondrial precipitate (PR) and supernatant (SN) fractions of SNPC, CAU and CC but higher in the SN from SNPR. 2) CC shows the highest activity of AAP and its activity is mainly located in the SN from all areas. 3) The activity of PAP is comparatively higher in SNPC and it is exclusively located in the SN from all areas. 4) gammaGTP activity is similar in all areas but its predominance is in the SN for SNPC and SNPR, and in the PR for CAU and CC. 5) CAU has higher PEP activity (higher in the PR) than CC (higher in the SN); no activity is detected in the substantia nigra. 6) The activity of a "Trypsin-like" peptidase is the highest in SNPC and SNPR; this activity have some predominance in the SN and higher predominance in the same fraction from CAU and CC. PMID:10591406

  13. A novel x-ray detector design with higher DQE and reduced aliasing: Theoretical analysis of x-ray reabsoprtion in detector converter material

    Nano, Tomi; Escartin, Terenz; Karim, Karim S.; Cunningham, Ian A.

    2016-03-01

    The ability to improve visualization of structural information in digital radiography without increasing radiation exposures requires improved image quality across all spatial frequencies, especially at high frequencies. The detective quantum efficiency (DQE) as a function of spatial frequency quantifies image quality given by an x-ray detector. We present a method of increasing DQE at high spatial frequencies by improving the modulation transfer function (MTF) and reducing noise aliasing. The Apodized Aperature Pixel (AAP) design uses a detector with micro-elements to synthesize desired pixels and provide higher DQE than conventional detector designs. A cascaded system analysis (CSA) that incorporates x-ray interactions is used for comparison of the theoretical MTF, noise power spectrum (NPS), and DQE. Signal and noise transfer through the converter material is shown to consist of correlated an uncorrelated terms. The AAP design was shown to improve the DQE of both material types that have predominantly correlated transfer (such as CsI) and predominantly uncorrelated transfer (such as Se). Improvement in the MTF by 50% and the DQE by 100% at the sampling cut-off frequency is obtained when uncorrelated transfer is prevalent through the converter material. Optimizing high-frequency DQE results in improved image contrast and visualization of small structures and fine-detail.

  14. 美国儿科学会胎儿和新生儿委员会《新生儿低血糖症筛查和后续管理指南(2011年版)》解读%Interpretation of Practical Guide and Algorithm for the Screening and Subsequent Management of Neonatal Hypoglycemia formulated by the American Academy of Pediatrics Committee on Fetus and Newborn

    陈昌辉; 李茂军; 吴青; 石伟

    2011-01-01

    美国儿科学会新生儿专业委员会制定了2011年版新生儿低血糖症筛查和后续管理指南(简称指南).除高危新生儿外,指南不推荐常规监测血糖.2011年3月指南由Adamkin DH及其同事在《Pediatrics》发表,内容包括哪些新生儿需要筛查、何时筛查、实验室资料、临床体征和管理.%The American Academy of Pediatrics (AAP) Committee on Fetus and Newborn has waged an opinion on neonatal hypoglycemia and produced a Practical Guide and Algorithm for the Screening and Subsequent Management of Neonatal Hypoglycemia (hereinafter, "guideline") . The guidelines recommend against routine glucose monitoring except for high-risk infants. The AAP guideline, reported in the March issue of Pediatrics by David H. Adamkin, MD, and colleagues, includes which infants to screen, when to screen, laboratory data, clinical signs, and management

  15. Effects of large river dam regulation on bacterioplankton community structure.

    Ruiz-González, Clara; Proia, Lorenzo; Ferrera, Isabel; Gasol, Josep M; Sabater, Sergi

    2013-05-01

    Large rivers are commonly regulated by damming, yet the effects of such disruption on prokaryotic communities have seldom been studied. We describe the effects of the three large reservoirs of the Ebro River (NE Iberian Peninsula) on bacterioplankton assemblages by comparing several sites located before and after the impoundments on three occasions. We monitored the abundances of several bacterial phylotypes identified by rRNA gene probing, and those of two functional groups (picocyanobacteria and aerobic anoxygenic phototrophic bacteria-AAPs). Much greater numbers of particles colonized by bacteria were found in upstream waters than downstream sites. Picocyanobacteria were found in negligible numbers at most sites, whereas AAPs constituted up to 14% of total prokaryotes, but there was no clear effect of reservoirs on the spatial dynamics of these two groups. Instead, damming caused a pronounced decline in Betaproteobacteria, Gammaproteobacteria and Bacteroidetes from upstream to downstream sites, whereas Alphaproteobacteria and Actinobacteria significantly increased after the reservoirs. Redundancy analysis revealed that conductivity, temperature and dissolved inorganic nitrogen were the environmental predictors that best explained the observed variability in bacterial community composition. Our data show that impoundments exerted significant impacts on bacterial riverine assemblages and call attention to the unforeseen ecological consequences of river regulation. PMID:23278359

  16. Spatial Variation of the Relationship between PM2.5 Concentrations and Meteorological Parameters in China

    Gang Lin

    2015-01-01

    Full Text Available Epidemiological studies around the world have reported that fine particulate matter (PM2.5 is closely associated with human health. The distribution of PM2.5 concentrations is influenced by multiple geographic and socioeconomic factors. Using a remote-sensing-derived PM2.5 dataset, this paper explores the relationship between PM2.5 concentrations and meteorological parameters and their spatial variance in China for the period 2001–2010. The spatial variations of the relationships between the annual average PM2.5, the annual average precipitation (AAP, and the annual average temperature (AAT were evaluated using the Geographically Weighted Regression (GWR model. The results indicated that PM2.5 had a strong and stable correlation with meteorological parameters. In particular, PM2.5 had a negative correlation with precipitation and a positive correlation with temperature. In addition, the relationship between the variables changed over space, and the strong negative correlation between PM2.5 and the AAP mainly appeared in the warm temperate semihumid region and northern subtropical humid region in 2001 and 2010, with some localized differences. The strong positive correlation between the PM2.5 and the AAT mainly occurred in the mid-temperate semiarid region, the humid, semihumid, and semiarid warm temperate regions, and the northern subtropical humid region in 2001 and 2010.

  17. Measuring diagnostic accuracy of imaging parameters in pelvic lipomatosis

    Zhang, Yudong, E-mail: pku_zyd06@163.com [Department of Radiology, Peking University First Hospital, Beijing (China); Wu, Shiliang, E-mail: wushiliangjsh@263.net [Department of Urology, Peking University First Hospital, Beijing (China); Xi, Zhijun, E-mail: xizhijun@hsc.pku.edu.cn [Department of Urology, Peking University First Hospital, Beijing (China); Wang, Xiaoying, E-mail: cjr.wangxiaoying@vip.163.com [Department of Radiology, Peking University First Hospital, Beijing (China); Jiang, Xuexiang, E-mail: cjr.jxx@vip.163.com [Department of Radiology, Peking University First Hospital, Beijing (China)

    2012-11-15

    Objectives: To study whether the individual radiological findings can help predict diagnosis of pelvic lipomatosis (PL) or, specifically appreciate its progression. Methods: Data from 32 clinically proven cases of PL and 25 controls were collected. Two reviewers were recruited for a blinded evaluation, image features were recorded in terms of: (1) bladder shape; (2) bladder-rectosigmoid morphological indexes including ratio of superior-inferior to anterior-posterior length of bladder (SI/AP), angle between anterior and posterior wall (AAP), relative length of posterior urethra (rLPU), angle between bladder and seminal vesicle (ABS) and rectosigmoid morphological index (RMI); (3) secondary complications. Results were evaluated by an unpaired t test and ROC analysis. Results: The sensitivity and specificity were 40.6% and 100% for pear and banana-shaped bladder, 62.5% and 100% for SI/AP, 40.6% and 100% for AAP, 62.5% and 100% for ABS, 78.1% and 72% for rLPU, 59.4% and 96% for RMI, respectively. These radiological findings partially correlated with the severity of disease weighted by hydronephrosis and treatment grade. Image analysis demonstrated high prevalence of glandular cystitis (100%) and hydronephrosis (73.4%). Conclusion: We conclude that PL is a progressive disease involving multiple pelvic organs with high prevalence of intractable cystitis and hydronephrosis. The imaging characteristics can help predict diagnosis and, specifically appreciate progression.

  18. Organic Phosphorus in Shallow Lake Sediments in Middle and Lower Reaches of the Yangtze River Area in China

    JIN Xiang-Can; WANG Sheng-Rui; CHU Jian-Zhou; WU Feng-Chang

    2008-01-01

    Thirteen sediment core samples (0-10 cm) were taken from the seven lakes in the middle and lower reaches of the Yangtze River to determine the contents and distributions of organic phosphorus (P) fractions in the sediments of the shallow lakes in the area.The organic P fractions in the sediments were in the order of moderately labile organic P (MLOP) > moderately resistant organic P (MROP) > highly resistant organic P (HROP) > labile organic P (LOP),with average proportional ratios of 13.2:2.8:1.3:1.0.LOP,MLOP,and MROP were significantly related to the contents of total organic carbon (TOC),water-soluble P (WSP),algal-available P (AAP),NaHCO3-extractable P (Olsen-P),total P (TP),organic P (OP),and inorganic P (IP).However,HROP was significantly related to OP and weakly correlated with TOC,WSP,AAP,Olsen-P,TP or IP.This suggested that organic P,especially LOP and MLOP in sediments,deserved even greater attention than IP in regards to lake eutrophication.In terms of organic P,sediments were more hazardous than soils in lake eutrophication.Although OP concentrations were higher in moderately polluted sediment than those in heavily polluted sediment,LOP and MLOP were higher in the heavily polluted sediment,which indicated that heavily polluted sediment was more hazardous than moderately polluted sediment in lake eutrophication.

  19. Antioxidant activity in selected Slovenian organic and conventional crops

    Manca KNAP

    2015-12-01

    Full Text Available The demand for organically produced food is increasing. There is widespread belief that organic food is substantially healthier and safer than conventional food. According to literature organic food is free of phytopharmaceutical residues, contain less nitrates and more antioxidants. The aim of the present study was to verify if there are any differences in the antioxidant activity between selected Slovenian organic and conventional crops. Method of DPPH (2,2-diphenyl-1-picryhydrazyl was used to determine the antioxidant activity of 16 samples from organic and conventional farms. The same varieties of crops were analysed. DPPH method was employed to measure the antioxidant activity of polar antioxidants (AAp and antioxidant activity of fraction in ethyl acetate soluble antioxidants (EA AA. Descriptive statistics and variance analysis were used to describe differences between farming systems. Estimated differences between interactions for the same crop and different farming practice were mostly not statistically significant except for the AAp for basil and beetroot. Higher statistically significant values were estimated for conventional crops. For the EA AA in broccoli, cucumber, rocket and cherry statistically significant higher values were estimated for organic production.

  20. Enhanced stability of multilayer graphene-supported catalysts for polymer electrolyte membrane fuel cell cathodes

    Marinkas, A.; Hempelmann, R.; Heinzel, A.; Peinecke, V.; Radev, I.; Natter, H.

    2015-11-01

    One of the biggest challenges in the field of polymer electrolyte membrane fuel cells (PEMFC) is to enhance the lifetime and the long-term stability of PEMFC electrodes, especially of cathodes, furthermore, to reduce their platinum loading, which could lead to a cost reduction for efficient PEMFCs. These demands could be achieved with a new catalyst support architecture consisting of a composite of carbon structures with significant different morphologies. A highly porous cathode catalyst support layer is prepared by addition of various carbon types (carbon black particles, multi-walled carbon nanotubes (MWCNT)) to multilayer graphene (MLG). The reported optimized cathodes shows extremely high durability and similar performance to commercial standard cathodes but with 89% lower Pt loading. The accelerated aging protocol (AAP) on the membrane electrode assemblies (MEA) shows that the presence of MLG increases drastically the durability and the Pt-extended electrochemical surface area (ECSA). In fact, after the AAP slightly enhanced performance can be observed for the MLG-containing cathodes instead of a performance loss, which is typical for the commercial carbon-based cathodes. Furthermore, the presence of MLG drastically decreases the ECSA loss rate. The MLG-containing cathodes show up to 6.8 times higher mass-normalized Pt-extended ECSA compared to the commercial standard systems.

  1. Carotenoid charge transfer states and their role in energy transfer processes in LH1-RC complexes from aerobic anoxygenic phototrophs.

    Šlouf, Václav; Fuciman, Marcel; Dulebo, Alexander; Kaftan, David; Koblížek, Michal; Frank, Harry A; Polívka, Tomáš

    2013-09-26

    Light-harvesting complexes ensure necessary flow of excitation energy into photosynthetic reaction centers. In the present work, transient absorption measurements were performed on LH1-RC complexes isolated from two aerobic anoxygenic phototrophs (AAPs), Roseobacter sp. COL2P containing the carotenoid spheroidenone, and Erythrobacter sp. NAP1 which contains the carotenoids zeaxanthin and bacteriorubixanthinal. We show that the spectroscopic data from the LH1-RC complex of Roseobacter sp. COL2P are very similar to those previously reported for Rhodobacter sphaeroides, including the transient absorption spectrum originating from the intramolecular charge-transfer (ICT) state of spheroidenone. Although the ICT state is also populated in LH1-RC complexes of Erythrobacter sp. NAP1, its appearance is probably related to the polarity of the bacteriorubixanthinal environment rather than to the specific configuration of the carotenoid, which we hypothesize is responsible for populating the ICT state of spheroidenone in LH1-RC of Roseobacter sp. COL2P. The population of the ICT state enables efficient S1/ICT-to-bacteriochlorophyll (BChl) energy transfer which would otherwise be largely inhibited for spheroidenone and bacteriorubixanthinal due to their low energy S1 states. In addition, the triplet states of these carotenoids appear well-tuned for efficient quenching of singlet oxygen or BChl-a triplets, which is of vital importance for oxygen-dependent organisms such as AAPs. PMID:23130956

  2. Formas agudas de periodontitis Acute conditions of periodontal disease

    L. Pérez-Salcedo

    2008-04-01

    Full Text Available La clasificación de las Enfermedades Periodontales ha cambiado en las últimas décadas. En la clasificación la AAP de 1989 la periodontitis necrotizante ocupaba el cuarto lugar. En el Workshop Europeo de 1993 la periodontitis necrotizante aparece en el grupo de los descriptores primarios. Según el Internacional Workshop for a Classification of Periodontal Diseases and Conditions 1999 en el que se revisó y se modificó la clasificación de las patologías periodontales, las enfermedades periodontales necrotizantes ocupan el punto cinco, diferenciándose entre Gingivitis Necrotizante y Periodontitis Necrotizante. Y se añade en la clasificación el grupo de abscesos periodontales. En este artículo de revisión vamos a profundizar acerca de las formas agudas de periodontitis.The Periodontal Diseases classification had changed in the last decades. In AAP classification of 1989 the necrotize was in the 4th position. In the European Workshop was in the group of primary descriptors. According to the International Workshop for a Classification of Periodontal Diseases and Conditions 1999, review and modificated the classification of periodontal pathologies, the periodontal necrotize diseases are in the 5th position, distinguishing between Necrotize Gingivitis and Necrotize Periodontitis. And Peridontal Abscesses was add to the classification. In this paper we are going to review about the acute forms of Periodontal Diseases.

  3. Design and characterization of an acid-activated antimicrobial peptide.

    Li, Lina; He, Jian; Eckert, Randal; Yarbrough, Daniel; Lux, Renate; Anderson, Maxwell; Shi, Wenyuan

    2010-01-01

    Dental caries is a microbial biofilm infection in which the metabolic activities of plaque bacteria result in a dramatic pH decrease and shift the demineralization/remineralization equilibrium on the tooth surface towards demineralization. In addition to causing a net loss in tooth minerals, creation of an acidic environment favors growth of acid-enduring and acid-generating species, which causes further reduction in the plaque pH. In this study, we developed a prototype antimicrobial peptide capable of achieving high activity exclusively at low environmental pH to target bacterial species like Streptococcus mutans that produce acid and thrive under the low pH conditions detrimental for tooth integrity. The features of clavanin A, a naturally occurring peptide rich in histidine and phenylalanine residues with pH-dependent antimicrobial activity, served as a design basis for these prototype 'acid-activated peptides' (AAPs). Employing the major cariogenic species S. mutans as a model system, the two AAPs characterized in this study exhibited a striking pH-dependent antimicrobial activity, which correlated well with the calculated charge distribution. This type of peptide represents a potential new way to combat dental caries. PMID:19878192

  4. Transmission of Pantoea ananatis and P. agglomerans, causal agents of center rot of onion (Allium cepa), by onion thrips (Thrips tabaci) through feces.

    Dutta, B; Barman, A K; Srinivasan, R; Avci, U; Ullman, D E; Langston, D B; Gitaitis, R D

    2014-08-01

    Frankliniella fusca, the tobacco thrips, has been shown to acquire and transmit Pantoea ananatis, one of the causal agents of the center rot of onion. Although Thrips tabaci, the onion thrips, is a common pest of onions, its role as a vector of P. ananatis has been unknown. The bacterium, P. agglomerans, is also associated with the center rot of onion, but its transmission by thrips has not been previously investigated. In this study, we investigated the relationship of T. tabaci with P. ananatis and P. agglomerans. Surface-sterilized T. tabaci were provided with various acquisition access periods (AAP) on onion leaves inoculated with either P. ananatis or P. agglomerans. A positive exponential relationship was observed between thrips AAP duration and P. ananatis (R² = 0.967; P = 0.023) or P. agglomerans acquisition (R² = 0.958; P = 0.017). Transmission experiments conducted with T. tabaci adults indicated that 70% of the seedlings developed center rot symptoms 15 days after inoculation. Immunofluorescence microscopy with antibodies specific to P. ananatis revealed that the bacterium was localized only in the gut of T. tabaci adults. Mechanical inoculation of onion seedlings with fecal rinsates alone produced center rot but not with salivary secretions. Together these results suggested that T. tabaci could efficiently transmit P. ananatis and P. agglomerans. PMID:24548212

  5. Attachment disorganization in different clinical groups: What underpins unresolved attachment?

    Juen Florian

    2013-01-01

    Full Text Available This paper summarizes findings and clinical implications of research on attachment disorganization in diverse clinical groups. Disorganized/unresolved attachment is overrepresented in these groups compared to healthy control participants, but disorder specific characteristics of this attachment pattern are still poorly understood. The focus of this study was to explore defensive processes in participants whose narratives were classified as disorganized/unresolved using the Adult Attachment Projective Picture System (AAP. Besides the predominance of disorganized attachment, clinical participants demonstrated more “segregated system material” especially in stories representing aloneness and more “Personal Experience material” compared to healthy individuals. Within the disorganized/ unresolved clinical individuals, BPD and PTSD patients showed the highest proportion of attachment disorganization and were less able to use other attachment-related defenses to maintain organized. Furthermore, PTSD patients were emotionally overwhelmed by the projective attachment scenes compared to the other clinical groups as indexed by an incapacity to complete sections of the AAP. BPD and addicted patients were characterized by a high degree of self-other boundary confusion. Depressive and schizophrenic patients showed a high overall defensive intensity to remain organized.

  6. Herpesviruses and breast milk

    C. Pietrasanta

    2014-06-01

    Full Text Available Breast milk has always been the best source of nourishment for newborns. However, breast milk can carry a risk of infection, as it can be contaminated with bacterial or viral pathogens. This paper reviews the risk of acquisition of varicella-zoster virus (VZV and cytomegalovirus (CMV, herpesviruses frequently detected in breastfeeding mothers, via breast milk, focusing on the clinical consequences of this transmission and the possible strategies for preventing it. Maternal VZV infections are conditions during which breastfeeding may be temporarily contraindicated, but expressed breast milk should always be given to the infant. CMV infection acquired through breast milk rarely causes disease in healthy term newborns; an increased risk of CMV disease has been documented in preterm infants. However, the American Academy of Pediatrics (AAP does not regard maternal CMV seropositivity as a contraindication to breastfeeding; according to the AAP, in newborns weighing less than 1500 g, the decision should be taken after weighing the benefits of breast milk against the risk of transmission of infection. The real efficacy of the different methods of inactivating CMV in breast milk should be compared in controlled clinical trials, rigorously examining the negative consequences that each of these methods can have on the immunological and nutritional properties of the milk itself, with a view to establish the best risk-benefit ratio of these strategies before they are recommended for use in clinical practice.

  7. Accelerated automatic phase shift method for DG anti-islanding%分布式发电反孤岛控制的自动加速相移方法

    张榴晨; Mary Kaye; Chris Diduch; 尹俊

    2014-01-01

    分布式发电反孤岛技术的最新研究进展表明,相移法能有效用于具有逆变器的分布式发电系统反孤岛保护。介绍滑模频率偏移(SMS)、主动频率偏移(AFD)和带正反馈的主动频率偏移(AFDPF)。自动相移法(APS)是一种改进的 SMS法,减少了 SMS的测区盲区(NDZ)。但稳定孤岛效应的频率确定困难,而且 APS运行慢,并可能在某些特定条件下失效。基于目前存在的难题,该文提出一种改进的自动加速相移算法(AAPS),矫正孤岛效应下的附加相位漂移,评价总电压相位漂移对电压周期的影响。该算法在孤岛发生时,会引起快速的相移,但在电网互联时只会引起极小的相位。孤岛发生时,该算法通过逆变器相移正反馈实现。仿真和实验结果证明了 AAPS算法的有效性。先进的 AAPS已经被应用于风能和太阳能系统的商业并网逆变器中,并符合 UL/CSA认证标准要求。各种条件下的认证测试结果表明 APPS算法可以在极短的时间内断开逆变器。%Recent developments in anti-islanding techniques have demonstrated that phase shift methods are very effective for anti-islanding protection in inverter-based distributed generation (DG)systems.Slide-mode frequency shift (SMS),active frequency drift (AFD),and active fre-quency drift with positive feedback (AFDPF)are presented in this paper.The automatic phase shift (APS)method is a modified SMS method that reduces the Non-Detection Zone (NDZ)of the SMS technique.However,it is difficult to determine a stable islanding frequency,and the APS algorithm may act slowly or fail under certain load conditions.An improved accelerated au-tomatic phase shift (AAPS)algorithm is proposed in this paper to regulate the additional phase shift when an island condition is hypothesized and to evaluate the impact of the total phase shift on the voltage period .The new algorithm causes a quick phase shift when an island occurs

  8. Management of acute gastroenteritis in healthy children in Lebanon - A national survey

    Nahida Rifai

    2010-11-01

    Full Text Available Background: Acute gastroenteritis remains a common condition among infants and children throughout the world. In 1996, The American Academy of Pediatrics (AAP revised its recommendations for the treatment of infants and children with acute gastroenteritis. Aim: The purpose of this survey was to determine how closely current treatment among Lebanese pediatricians compares with the AAP recommendations and to determine the impact of such management on the healthcare system. Patients and Methods: The outline of the study was based on a telephone questionnaire that addressed the management of healthy infants and children below five years of age with acute gastroenteritis complicated by mild to moderate dehydration. In addition, the costs of medical treatment and requested laboratory studies were calculated. Results: A total of 238 pediatricians completed the questionnaire. Most pediatricians prescribed Oral Rehydration Solutions (ORS for rehydration (92.4%, advised breastfeeding during acute gastroenteritis (81.5%, and avoided parenteral rehydration for mild to moderate dehydration (89.1%. In addition to ORS, oral fluids such as soda, juices, and rice water were allowed for rehydration by 43.7% of pediatricians. Thirty-one percent of pediatricians delayed re-feeding for more than 6 hours after initiation of rehydration. Only 32.8% of pediatricians kept their patients on regular full-strength formulas, and only 21.8% permitted full-calorie meals for their patients. 75.4% of pediatricians did not order any laboratory studies in cases of mild dehydration and 50.4% did not order any laboratory studies for moderate dehydration. Stool analysis and culture were ordered by almost half of the pediatricians surveyed. Seventy-seven percent prescribed anti-emetics, 61% prescribed probiotics, 26.3% prescribed antibiotics systematically and local antiseptic agents, 16.9% prescribed zinc supplements, and 11% percent prescribed antidiarrheal agents. Conclusion

  9. Management of acute gastroenteritis in healthy children in Lebanon - A national survey

    Aouni Alameddine

    2010-01-01

    Full Text Available Background: Acute gastroenteritis remains a common condition among infants and children throughout the world. In 1996, The American Academy of Pediatrics (AAP revised its recommendations for the treatment of infants and children with acute gastroenteritis. Aim: The purpose of this survey was to determine how closely current treatment among Lebanese pediatricians compares with the AAP recommendations and to determine the impact of such management on the healthcare system. Patients and Methods: The outline of the study was based on a telephone questionnaire that addressed the management of healthy infants and children below five years of age with acute gastroenteritis complicated by mild to moderate dehydration. In addition, the costs of medical treatment and requested laboratory studies were calculated. Results: A total of 238 pediatricians completed the questionnaire. Most pediatricians prescribed Oral Rehydration Solutions (ORS for rehydration (92.4%, advised breastfeeding during acute gastroenteritis (81.5%, and avoided parenteral rehydration for mild to moderate dehydration (89.1%. In addition to ORS, oral fluids such as soda, juices, and rice water were allowed for rehydration by 43.7% of pediatricians. Thirty-one percent of pediatricians delayed re-feeding for more than 6 hours after initiation of rehydration. Only 32.8% of pediatricians kept their patients on regular full-strength formulas, and only 21.8% permitted full-calorie meals for their patients. 75.4% of pediatricians did not order any laboratory studies in cases of mild dehydration and 50.4% did not order any laboratory studies for moderate dehydration. Stool analysis and culture were ordered by almost half of the pediatricians surveyed. Seventy-seven percent prescribed anti-emetics, 61% prescribed probiotics, 26.3% prescribed antibiotics systematically and local antiseptic agents, 16.9% prescribed zinc supplements, and 11% percent prescribed antidiarrheal agents. Conclusion

  10. Time Ephemeris and Relativistic Scaling of Ephemerides

    Fukushima, Toshio

    2009-05-01

    Time ephemeris is the location-independent part of the transformation formula relating two time coordinates such as TCB and TCG. It is computed from the corresponding (space) ephemerides providing the relative motion of two spatial coordinate origins associated such as the motion of geocenter relative to the solar system barycenter. The time ephemerides are inevitablly needed in conducting a precise four-dimensional coordinate transformation among various spacetime coodrinate systems such as the GCRS and BCRS. Also, by means of the time average operation, it is useful in determining the information on scale conversion between the pair of coordinate systems, especially scale conversion factors such as LC. In 1995, we presented the first numerically-integrated time ephemeris, TE245, from JPL's planetary ephemeris DE245 (Fukushima 1995, A&Ap, 294, 895-906). Four years later, we updated it to TE405 associated with DE405 (Irwin and Fukushima 1999, A&Ap, 348, 642-652). The former gave an estimate of LC, the scale conversion factor between TCB and TCG, as 1.4808268457(10) x 10-8. Meanwhile the latter renewed it as 1.48082686741(200) x 10-8. Another four years later, by using a precise technique of time avarage, we improved the estimate as 1.4808268559(6) x 10-8 (Harada and Fukushima 2003, AJ, 126, 2557-2561). The main reasons of these uncertainties are the truncation effect in time average and the uncertainty of asteroids' perturbation. The former is a natural limitation caused by the finite length of numerical planetary ephemerides and the latter is due to the uncertainty of masses of some heavy asteroids. In the talk, we review the post-Newtonian formulas to integrate time ephemerides as well as some practical details on their numerical integration. Also, we explain two kinds of techniques of time average. One is a semi-numerical approach as explained in 1991 A&Ap article and the other is purely numerical as given in 2003 AJ paper.

  11. Value of the 14C-aminophenazone respiratory test: Inhibition of the demethylation function of the liver by cimetidine

    In 15 patients with healthy liver and peptic ulcer the demethylation capacity of the liver was determined by the 14C-aminophenazone breathing test before and during treatment with the H2 antagonist cimetidine (daily dose 1.0 g) measuring the expired 14CO2 (DPM/mmol CO2/70 kg body weight) before and 1 hour after intake. Influenced by cimetidine the mean 14CO2 value of 724.7 DPM (S.D. 127.7) decreased to 404.1 DPM (S.D. 153.1). The enzyme activities (ALAT, ASAT, AP, AAP, GGT, CHE) and the concentrations of serum bilirubin and serum albumin remained unchanged and did not correlate mutually and with the 14CO2 data. The aminophenazone test proved to be suitable technique for the recognition of disturbances of the MFO system. (author)

  12. A self-compartmentalizing hexamer serine protease from Pyrococcus horikoshii: substrate selection achieved through multimerization.

    Menyhárd, Dóra K; Kiss-Szemán, Anna; Tichy-Rács, Éva; Hornung, Balázs; Rádi, Krisztina; Szeltner, Zoltán; Domokos, Klarissza; Szamosi, Ilona; Náray-Szabó, Gábor; Polgár, László; Harmat, Veronika

    2013-06-14

    Oligopeptidases impose a size limitation on their substrates, the mechanism of which has long been under debate. Here we present the structure of a hexameric serine protease, an oligopeptidase from Pyrococcus horikoshii (PhAAP), revealing a complex, self-compartmentalized inner space, where substrates may access the monomer active sites passing through a double-gated "check-in" system, first passing through a pore on the hexamer surface and then turning to enter through an even smaller opening at the monomers' domain interface. This substrate screening strategy is unique within the family. We found that among oligopeptidases, a residue of the catalytic apparatus is positioned near an amylogenic β-edge, which needs to be protected to prevent aggregation, and we found that different oligopeptidases use different strategies to achieve such an end. We propose that self-assembly within the family results in characteristically different substrate selection mechanisms coupled to different multimerization states. PMID:23632025

  13. A Self-compartmentalizing Hexamer Serine Protease from Pyrococcus Horikoshii

    Menyhárd, Dóra K.; Kiss-Szemán, Anna; Tichy-Rács, Éva; Hornung, Balázs; Rádi, Krisztina; Szeltner, Zoltán; Domokos, Klarissza; Szamosi, Ilona; Náray-Szabó, Gábor; Polgár, László; Harmat, Veronika

    2013-01-01

    Oligopeptidases impose a size limitation on their substrates, the mechanism of which has long been under debate. Here we present the structure of a hexameric serine protease, an oligopeptidase from Pyrococcus horikoshii (PhAAP), revealing a complex, self-compartmentalized inner space, where substrates may access the monomer active sites passing through a double-gated “check-in” system, first passing through a pore on the hexamer surface and then turning to enter through an even smaller opening at the monomers' domain interface. This substrate screening strategy is unique within the family. We found that among oligopeptidases, a residue of the catalytic apparatus is positioned near an amylogenic β-edge, which needs to be protected to prevent aggregation, and we found that different oligopeptidases use different strategies to achieve such an end. We propose that self-assembly within the family results in characteristically different substrate selection mechanisms coupled to different multimerization states. PMID:23632025

  14. 何谓肠出血性大肠杆菌O104:H4及防治%What is enterohemorrhagic Escherichia coli O104:H4 and how to prevent

    任成山; 赵晓晏

    2011-01-01

    @@ 所谓肠出血性大肠杆菌O104:H4(enterohemorrhagic E.coli,O104:H4),是由德国分离的一种新菌珠O104:H4型大肠杆菌,含有志贺毒素2(vtx2a)的基因,没有(enterohemorrhagic Escherichia coli,EHEC)溶血素、志贺毒素1、肠致病性大肠埃希菌(enteropathogenic Escherichia coli)基因毒力岛基因;但含有肠集聚性粘附大肠杆菌(enteroaggragtive Escherichia coli)(第5类致泻性大肠杆菌)毒力质粒上的3个基因:aatA、aggR、aap[1].

  15. DOI发展现状及其在学术出版中的意义

    杨小平

    2008-01-01

    @@ 1 DOI简介 DOI(Digital Object Identifier),数字对象唯一标志符.DOI系统是美国出版商协会(AmericanAssociate of Publishing,AAP)1994年成立的非赢利性组织国际DOI基金会(International DOI Fun-dation,IDF)在美国国家创新研究组织(The Cor-poration for National Research Institute,CNRI)的配合下,专门研究设计出来的一种既能保护知识产权又能保障版权所有者商业利益的系统[1].

  16. DOI简介

    2014-01-01

    <正>DOI(Digital Object Identifier),即文献数字对象识别号,为美国出版商协会(The Association of American Publishers,AAP)于1994年建立,1997年成为数字资源命名的一项标准。DOI能让学术文章永久寻址,实现文献永续存在。《厦门大学法律评论》从2013年起,通过台湾元照出版公司提供的平台,正式加入DOI系统,所有本刊刊发的文章将正式获得全球唯一且永续的"身份证号"。

  17. DOI简介

    2013-01-01

    <正>DOI(Digital Object Identifier),即文献数字对象识别号,为美国出版商协会(The Association of American Publishers,AAP)于1994年建立;1997年成为数字资源命名的一项标准。DOI能让学术文章永久寻址,实现文献永续存在。《厦门大学法律评论》从2013年起,通过台湾元照出版公司提供的平台,正式加入DOI系统,所有本刊刊发的文章将正式获得全球唯一且永续的"身份证号"。

  18. The antiarrhythmic peptide analog rotigaptide (ZP123) stimulates gap junction intercellular communication in human osteoblasts and prevents decrease in femoral trabecular bone strength in ovariectomized rats

    Jørgensen, Niklas Rye; Teilmann, Stefan Cuoni; Henriksen, Zanne;

    2005-01-01

    this study was to examine whether antiarrhythmic peptides, which are small peptides increasing gap junctional conductivity, show specific binding to osteoblasts and investigate the effect of the stable analog rotigaptide (ZP123) on gap junctional intercellular communication in vitro and on bone mass...... and strength in vivo. Cell coupling and calcium signaling were assessed in vitro on human, primary, osteoblastic cells. In vivo effects of rotigaptide on bone strength and density were determined 4 wk after ovariectomy in rats treated with either vehicle, sc injection twice daily (300 nmol per......]AAP10 could not be shown. Studies of the effects of rotigaptide on propagation of intercellular calcium waves and cell-to-cell coupling demonstrated that 10 nM rotigaptide produced a small increase in intercellular communication during physiological conditions (+4.5+/-1.6% vs. vehicle; P...

  19. 印度把握“极端之地”的未来——ZeeTV:营销“印度象”的王牌

    刘嘉偌; 高远(摄影)

    2007-01-01

    著名电视人拉杰特-沙马认为,类似于AapKiAdalat(电视政论节目)这样的高质量节目,以及通过技术把精彩娱乐带到消费者面前,可谓Zee电视台成功的两大至要。他还坚持,这家电视台不仅是商业意义上的成功,也直接推动了印度的改革开放,“让印度人民了解了外部世界,也让世界了解印度。某种程度上,可以说是营销印度的先锋。”

  20. Gene : CBRC-GGOR-01-1364 [SEVENS

    Full Text Available bradykinin receptor B1 [Homo sapiens] sp|P46663|BKRB1_HUMAN RecName: Full=B1 bradykinin receptor; AltName: ...Full=BK-1 receptor; Short=B1R emb|CAB45650.1| bradykinin B1 receptor [Homo sapiens] dbj|BAC06112.1| seven tr...ansmembrane helix receptor [Homo sapiens] gb|AAH34705.1| Bradykinin receptor B1 [Homo sapi...ens] gb|AAP32296.1| bradykinin receptor B1 [Homo sapiens] gb|EAW81632.1| bradykinin receptor B1 [Homo sapi...1 [synthetic construct] dbj|BAF84659.1| unnamed protein product [Homo sapiens] 1e

  1. Atrial fibrillation in rats induced by rapid transesophageal atrial pacing during brief episodes of asphyxia: A new in vivo model

    Haugan, K.; Lam, Henrik Rye; Knudsen, C. B.;

    2004-01-01

    anesthetized male Sprague-Dawley rats. AF was reproducibly induced in 81% of the rats. The presence of AF was associated with an increased heart rate, and a decreased blood pressure. Treatment with amiodarone, D,L-sotalol, flecainide, and propranolol all reduced duration of AF, whereas verapamil treatment was...... associated with a marked profibrillatory effect. Increasing gap junction intracellular communication using the antiarrhythmic peptide analogue AAP 10 did not affect AF duration. Basal plasma level of epinephrine and norepinephrine were increased 5- to 20-fold relative to values reported by others, but...... unchanged following 35 seconds of asphyxia. The results from our study demonstrate that the rat model shares several clinical key characteristics with human AF: (1) hemodynamic response to AF; (2) increased autonomic tone; (3) antiarrhythmic effects of clinically used drugs; (4) profibrillatory effect of...

  2. Alergia alimentar: atualização prática do ponto de vista gastroenterológico Food allergy: a practical update from the gastroenterological viewpoint

    Cristina Targa Ferreira

    2007-02-01

    Full Text Available OBJETIVOS: Apresentar uma revisão atualizada e crítica sobre alergias alimentares, focando principalmente em tratamento e prevenção. FONTES DOS DADOS: Revisão da literatura publicada obtida através do banco de dados MEDLINE, sendo selecionados os mais atuais e representativos do tema (2000-2006. A pesquisa incluiu os sites da European Society of Pediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN e American Academy of Pediatrics (AAP. SÍNTESE DOS DADOS: A prevalência de doenças alérgicas tem aumentado nas últimas décadas e alergia alimentar parece fazer parte desse aumento. Alergia alimentar é muito mais comum em pediatria e apresenta impacto médico, financeiro e social significativos em crianças menores e suas famílias. Tratamento e prevenção da alergia alimentar são desafios maiores do ponto de vista da saúde pública e para as comunidades médica e científica. Há muita informação incorreta e condutas médicas discutíveis nessa área. Apresentamos e discutimos as recomendações publicadas pelos Comitês de Nutrição da ESPGHAN juntamente com a Sociedade Européia Pediátrica de Alergologia e Imunologia Clínica (ESPACI e AAP. CONCLUSÃO: Excesso de diagnósticos de alergia alimentar é bastante prevalente. Há necessidade de uniformização de definições e procedimentos diagnósticos. O objetivo primário do manejo deve ser o de instituir medidas efetivas de prevenção das alergias alimentares. Há necessidade de métodos precisos para confirmar ou excluir o diagnóstico. Os pacientes necessitam tratamento apropriado através da eliminação de alimentos que causam sintomas, ao mesmo tempo evitando os efeitos adversos nutricionais e o custo de dietas inadequadas.OBJECTIVE: To present an up-to-date and critical review regarding food allergies, focusing mainly on treatment and prevention. SOURCES: Review of published literature searched on MEDLINE database; those data which were the most up-to-date and

  3. Exploring teacher influence on the lives of students from diverse elementary schools in a rural Midwestern community

    Knoell, Christopher M.; Crow, Sherry R.

    2013-01-01

    Įvadas. Praktikai ir mokslininkai mokinių pažangumo ir adekvataus ankstyvo progreso (AAP) srityje ieško strategijų, kaip palaikyti tinkamą mokinių akademinę pažangą. Viena iš sričių, kurioje praktikai galėtų gauti daugiau informacijos, – mokytojų ir mokinių santykiai skirtingo tipo mokyklose ir situacijose arba tais atvejais, kai mokinių gebėjimai yra skirtingi. Tikslas. Šio tyrimo tikslas – palyginti, kokį poveikį mokytojai turi skurdžiai ir turtingai gyvenančių vaikų mokyklų penktos klasės ...

  4. An Arginine Deprivation Response Pathway Is Induced in Leishmania during Macrophage Invasion.

    Adele Goldman-Pinkovich

    2016-04-01

    Full Text Available Amino acid sensing is an intracellular function that supports nutrient homeostasis, largely through controlled release of amino acids from lysosomal pools. The intracellular pathogen Leishmania resides and proliferates within human macrophage phagolysosomes. Here we describe a new pathway in Leishmania that specifically senses the extracellular levels of arginine, an amino acid that is essential for the parasite. During infection, the macrophage arginine pool is depleted due to its use to produce metabolites (NO and polyamines that constitute part of the host defense response and its suppression, respectively. We found that parasites respond to this shortage of arginine by up-regulating expression and activity of the Leishmania arginine transporter (LdAAP3, as well as several other transporters. Our analysis indicates the parasite monitors arginine levels in the environment rather than the intracellular pools. Phosphoproteomics and genetic analysis indicates that the arginine-deprivation response is mediated through a mitogen-activated protein kinase-2-dependent signaling cascade.

  5. An Arginine Deprivation Response Pathway Is Induced in Leishmania during Macrophage Invasion.

    Goldman-Pinkovich, Adele; Balno, Caitlin; Strasser, Rona; Zeituni-Molad, Michal; Bendelak, Keren; Rentsch, Doris; Ephros, Moshe; Wiese, Martin; Jardim, Armando; Myler, Peter J; Zilberstein, Dan

    2016-04-01

    Amino acid sensing is an intracellular function that supports nutrient homeostasis, largely through controlled release of amino acids from lysosomal pools. The intracellular pathogen Leishmania resides and proliferates within human macrophage phagolysosomes. Here we describe a new pathway in Leishmania that specifically senses the extracellular levels of arginine, an amino acid that is essential for the parasite. During infection, the macrophage arginine pool is depleted due to its use to produce metabolites (NO and polyamines) that constitute part of the host defense response and its suppression, respectively. We found that parasites respond to this shortage of arginine by up-regulating expression and activity of the Leishmania arginine transporter (LdAAP3), as well as several other transporters. Our analysis indicates the parasite monitors arginine levels in the environment rather than the intracellular pools. Phosphoproteomics and genetic analysis indicates that the arginine-deprivation response is mediated through a mitogen-activated protein kinase-2-dependent signaling cascade. PMID:27043018

  6. Gene : CBRC-MMUR-01-1104 [SEVENS

    Full Text Available CBRC-MMUR-01-1104 Novel UN B Odorant/olfactory and gustatory receptors OL502_MOUSE ...4e-41 82% ref|NP_666950.1| olfactory receptor 502 [Mus musculus] sp|Q8VG09|OL502_MOUSE RecName: Full=Olfactory receptor... 502; AltName: Full=Olfactory receptor 204-8 gb|AAL61019.1| olfactory receptor MOR204-8 [Mus musc...ulus] gb|AAP71001.1| olfactory receptor Olfr502 [Mus musculus] gb|AAI20679.1| Olfactory receptor... 502 [Mus musculus] gb|AAI19236.1| Olfactory receptor 502 [Mus musculus] gb|EDL16902.1| olfactory receptor

  7. Gene : CBRC-MMUR-01-0252 [SEVENS

    Full Text Available CBRC-MMUR-01-0252 Novel UN B Odorant/olfactory and gustatory receptors O1009_MOUSE ...3e-52 90% ref|NP_666783.1| olfactory receptor 1009 [Mus musculus] sp|Q8VFK1|O1009_MOUSE RecName: Full=Olfactory receptor... 1009; AltName: Full=Olfactory receptor 175-3 gb|AAL61188.1| olfactory receptor MOR175-3 [Mus mu...sculus] gb|AAP71461.1| olfactory receptor Olfr1009 [Mus musculus] emb|CAM16172.1| olfactory receptor... 1009 [Mus musculus] gb|EDL27334.1| olfactory receptor 1009 [Mus musculus] gb|AAI48239.1| Olfactory receptor

  8. Gene : CBRC-CPOR-01-0265 [SEVENS

    Full Text Available CBRC-CPOR-01-0265 Novel UN B Odorant/olfactory and gustatory receptors OL187_MOUSE ...2e-52 57% ref|NP_666434.1| olfactory receptor 187 [Mus musculus] sp|Q8VEX6|OL187_MOUSE Olfactory receptor 187 (Olfactory receptor... 183-8) gb|AAL61434.1| olfactory receptor MOR183-8 [Mus musculus] gb|AAP70777.1| olfactory receptor... Olfr187 [Mus musculus] gb|AAI19307.1| Olfactory receptor 187 [Mus mu...sculus] gb|AAI19305.1| Olfactory receptor 187 [Mus musculus] gb|EDK98203.1| olfactory receptor 187 [Mus musc

  9. Gene : CBRC-HSAP-19-0100 [SEVENS

    Full Text Available iens] dbj|BAA95414.1| G protein-coupled receptor C5L2 [Homo sapiens] gb|AAP23197.1| C5A anap...23872 /ug=Hs.534412 /len=1287 0.0 100% C5a-des-Arg anaphylatoxin Gi/o,Gq/11 MGNDSVSYEYGDYSDLSDRPVDCLDGACLAIDPLRVAP...CBRC-HSAP-19-0100 19 A Chemokines and chemotactic factors receptors C5ARL_HUMAN 0.0... 100% ref|NP_060955.1| G protein-coupled receptor 77 [Homo sapiens] sp|Q9P296|C5ARL_HUMAN C5a anaphylatoxin ...chemotactic receptor C5L2 (G-protein coupled receptor 77) gb|AAK12640.1|AF317655_1 G protein-coupled receptor [Homo sap

  10. Gene : CBRC-HSAP-14-0064 [SEVENS

    Full Text Available iens] emb|CAA60109.1| B2-bradykinin receptor gene [Homo sapiens] gb|AAP...CBRC-HSAP-14-0064 14 A Bradykinin receptors NONE BKRB2_HUMAN 0.0 100% ref|NP_000614....1| bradykinin receptor B2 [Homo sapiens] sp|P30411|BKRB2_HUMAN B2 bradykinin receptor (BK-2 receptor) (B2R)... gb|AAK56376.1|AF378542_1 bradykinin receptor B2 [Homo sapiens] emb|CAA60108.1| B-2 bradykinin receptor gene [Homo sap...32297.1| bradykinin receptor B2 [Homo sapiens] gb|AAH74895.1| Bradykinin receptor B2 [Homo sapiens] gb|AAH74

  11. NCBI nr-aa BLAST: CBRC-PMAR-01-0052 [SEVENS

    Full Text Available CBRC-PMAR-01-0052 ref|NP_843235.1| spore germination protein GerLA [Bacillus anthra...cis str. Ames] ref|YP_017339.1| spore germination protein gerla [Bacillus anthracis str. 'Ames Ancestor'] ref|YP_026952.1| spore germ...ination protein GerLA [Bacillus anthracis str. Sterne] gb|AAP24721.1| spore germination... protein GerLA [Bacillus anthracis str. Ames] gb|AAT29814.1| spore germination... protein GerLA [Bacillus anthracis str. 'Ames Ancestor'] gb|AAT53003.1| spore germination protein GerLA [Bacillus anthracis str. Sterne] NP_843235.1 0.45 25% ...

  12. NCBI nr-aa BLAST: CBRC-GGOR-01-0564 [SEVENS

    Full Text Available ; AltName: CD_antigen=CD66c; Flags: Precursor gb|AAA59907.1| non-specific cross reacting antigen [Homo sapiens...molecule 6 (non-specific cross reacting antigen) [Homo sapiens] gb|AAP88776.1| carcinoembryonic antigen-rela...ted cell adhesion molecule 6 (non-specific cross reacting antigen) [Homo sapiens] gb|AAX32506.1| carcinoembryonic antigen...CBRC-GGOR-01-0564 sp|P40199|CEAM6_HUMAN RecName: Full=Carcinoembryonic antigen-rela...ted cell adhesion molecule 6; AltName: Full=Normal cross-reacting antigen; AltName: Full=Non-specific crossreacting antigen

  13. Virulence factors profiles and ESBL production in Escherichia coli causing bacteremia in Peruvian children.

    Palma, Noemí; Gomes, Cláudia; Riveros, Maribel; García, Wilfredo; Martínez-Puchol, Sandra; Ruiz-Roldán, Lidia; Mateu, Judit; García, Coralith; Jacobs, Jan; Ochoa, Theresa J; Ruiz, Joaquim

    2016-09-01

    The presence of 25 virulence genes (VGs), genetic phylogroups, quinolone-resistance and Extended Spectrum β-lactamase (ESBL)-production was assessed in 65 Escherichia coli isolates from blood cultures in children Quinolone-susceptible (22 isolates - 33.8%) and ESBL-negative (31 isolates - 47.7%) isolates carried more VGs that their respective counterparts (5.7 vs. 4.7 and 5.3 vs. 4.4 respectively); the frequency of the fyuA, aat, aap, and hly genes significantly differed between quinolone-resistant and quinolone-susceptible isolates. Neonatal sepsis isolates tended to be more quinolone-resistant (P = 0.0697) and ESBL-producers (P = 0.0776). Early-onset neonatal sepsis isolates possessed a high number of VGs (5.2 VGs), especially in neonates of ≤1 day (5.9 VGs). PMID:27345125

  14. Asociación entre niveles de TNF-α en fluido crevicular gingival de dientes con periodontitis apical asintomática Levels of TNF-α increase in gingival crevicular fluid of teeth with asymptomatic apical periodontitis

    M Garrido Flores

    2011-12-01

    resorption, a hallmark of asymptomatic apical periodontitis (AAP. Gingival crevicular fluid (GCF has a great potential as a source of factors associated with osteoclastic activity. The aim of this study was to determine the levels of TNF- a in GCF of teeth with PAA and contralateral healthy controls. Methods: A total of 14 patients with clinical diagnosis of AAP were enrolled from the Clinic of Endodontics, Faculty of Dentistry, Universidad de Chile during 2009 and 30-second GCF samples were obtained with paper strips from AAP teeth and contralateral healthy controls. Total protein concentration and TNF- a levels were determined through bisciconitic acid method and ELISA assay, respectively. Paired t test and StataV11 software were used for statistical analysis. Results: Levels of TNF- a were significantly higher in GCF from teeth with AAP than controls when standardized by either 30s of sampling and total protein content. Conclusions: The present study provides preliminary data supporting that TNF- a levels in GCF reflect periapical status. Screening of TNF- a levels in GCF might represent a useful side-diagnostic tool to the monitoring of the apical status.

  15. Gene : CBRC-HSAP-12-0004 [SEVENS

    Full Text Available 100% ref|NP_004045.1| complement component 3a receptor 1 [Homo sapiens] sp|Q16581|C3AR_HUMAN C3a anaphylatox...in chemotactic receptor (C3a-R) (C3AR) emb|CAA97504.1| C3a anaphylatoxin receptor [Homo sapiens] gb|AAH20742....1| Complement component 3a receptor 1 [Homo sapiens] dbj|BAC06088.1| seven trans...membrane helix receptor [Homo sapiens] gb|AAP23198.1| complement component 3a receptor 1 [Homo sapiens] gb|A...AR13862.1| complement component 3a receptor 1 [Homo sapiens] gb|EAW88640.1| complement component 3a receptor

  16. Gene : CBRC-MMUS-04-0019 [SEVENS

    Full Text Available CBRC-MMUS-04-0019 4 A Odorant/olfactory and gustatory receptors O13J1_HUMAN 1e-145 ...82% ref|NP_062359.1| olfactory receptor 71 [Mus musculus] emb|CAB55597.1| olfactory receptor [Mus musculus] ...emb|CAB96152.1| olfactory receptor [Mus musculus] gb|AAL60904.1| olfactory receptor MOR262-4 [Mus musculus] ...gb|AAP70821.1| olfactory receptor Olfr71 [Mus musculus] emb|CAM17056.1| olfactory receptor 71 [Mus mus...culus] gb|AAI41883.1| Olfactory receptor 71 [Mus musculus] gb|AAI41893.1| Olfactory receptor 71 [Mus musculus

  17. Gene : CBRC-MMUS-09-0177 [SEVENS

    Full Text Available CBRC-MMUS-09-0177 9 A Odorant/olfactory and gustatory receptors OR6X1_HUMAN 1e-153 ...85% ref|NP_666826.1| olfactory receptor 986 [Mus musculus] gb|AAL61145.1| olfactory receptor MOR104-3 [Mus musculus...] gb|AAP71447.1| olfactory receptor Olfr986 [Mus musculus] gb|AAI16769.1| Olfactory receptor 986 [Mus musculus...] gb|AAI16771.1| Olfactory receptor 986 [Mus musculus] 1e-176 100% gnl|UG|Mm#S33850932 Mus mus...culus olfactory receptor 986, mRNA (cDNA clone MGC:151147 IMAGE:40126089), complete cd

  18. Gene : CBRC-MMUS-02-0321 [SEVENS

    Full Text Available CBRC-MMUS-02-0321 2 A Odorant/olfactory and gustatory receptors OR4B1_HUMAN 1e-145 ...82% ref|NP_667196.1| olfactory receptor 1270 [Mus musculus] gb|AAL60766.1| olfactory receptor MOR227-1 [Mus musculus...] gb|AAP71663.1| olfactory receptor Olfr1270 [Mus musculus] gb|AAI19114.1| Olfactory receptor 1270 [Mus musculus...] gb|AAI19116.1| Olfactory receptor 1270 [Mus musculus] emb|CAM17969....1| olfactory receptor 1270 [Mus musculus] gb|EDL27478.1| olfactory receptor 1270 [Mus musculus] 1e-170 100% gnl|UG|Mm#S34327486 Mus

  19. Gene : CBRC-MMUS-02-0365 [SEVENS

    Full Text Available CBRC-MMUS-02-0365 2 A Odorant/olfactory and gustatory receptors OR4KE_HUMAN 1e-104 ...59% ref|NP_667098.1| olfactory receptor 1301 [Mus musculus] gb|AAL60864.1| olfactory receptor MOR248-5 [Mus musculus...] gb|AAP71692.1| olfactory receptor Olfr1301 [Mus musculus] emb|CAM27187.1| olfactory receptor 1301 [Mus musculus...] gb|EDL27820.1| olfactory receptor 1301 [Mus musculus] 1e-177 100% gnl|UG|Mm#S9745172 Mus... musculus olfactory receptor 1301 (Olfr1301), mRNA /cds=p(1,939) /gb=NM_146887 /gi=221

  20. Gene : CBRC-MMUS-17-0163 [SEVENS

    Full Text Available CBRC-MMUS-17-0163 17 A Odorant/olfactory and gustatory receptors OR2BB_HUMAN 1e-101... 57% ref|NP_667042.1| olfactory receptor 133 [Mus musculus] gb|AAL60925.1| olfactory receptor MOR256-6 [Mus musculus...] gb|AAP71256.1| olfactory receptor Olfr133 [Mus musculus] gb|AAQ77061.1| olfactory receptor 133 [Mus musculus...] gb|AAI32058.1| Olfactory receptor 133 [Mus musculus] gb|AAI32060.1| O...lfactory receptor 133 [Mus musculus] gb|EDL23374.1| mCG64027 [Mus musculus] 1e-179 100% gnl|UG|Mm#S36660534 Mus musculus

  1. Gene : CBRC-MMUS-11-0013 [SEVENS

    Full Text Available CBRC-MMUS-11-0013 11 A Odorant/olfactory and gustatory receptors O2T27_HUMAN 1e-110... 60% ref|NP_667088.1| olfactory receptor 1395 [Mus musculus] gb|AAL60876.1| olfactory receptor MOR277-1 [Mus musculus...] gb|AAP71777.1| olfactory receptor Olfr1395 [Mus musculus] emb|CAM46261.1| olfactory receptor 1395 [Mus musculus...] gb|EDL33774.1| olfactory receptor 1395 [Mus musculus] 1e-180 100% gnl|UG|Mm#S9745164 Mus... musculus olfactory receptor 1395 (Olfr1395), mRNA /cds=p(1,954) /gb=NM_146877 /gi=22

  2. The impact of compulsory cycle helmet legislation on cyclist head injuries in New South Wales, Australia: a rejoinder.

    Rissel, Chris

    2012-03-01

    This paper challenges the conclusion of a recent paper by Walter et al. (Accident Analysis and Prevention 2011, doi:10.1016/j.aap.2011.05.029) reporting that despite numerous data limitations repealing the helmet legislation in Australia could not be justified. This conclusion is not warranted because of the limited time period used in their analysis and the lack of data beyond a few years before the introduction of legislation, the failure to adequately account for the effect of the phasing in of the legislation, the effect of the marked reduction in child cyclists, and the non-comparability of the pedestrian and cycling injuries and related lack of consideration of the severity of head injuries. The extent to which helmet legislation deters people from cycling is discussed. PMID:22269491

  3. Surveying Turkish high school and university students' attitudes and approaches to physics problem solving

    Balta, Nuri; Mason, Andrew J.; Singh, Chandralekha

    2016-06-01

    Students' attitudes and approaches to physics problem solving can impact how well they learn physics and how successful they are in solving physics problems. Prior research in the U.S. using a validated Attitude and Approaches to Problem Solving (AAPS) survey suggests that there are major differences between students in introductory physics and astronomy courses and physics experts in terms of their attitudes and approaches to physics problem solving. Here we discuss the validation, administration, and analysis of data for the Turkish version of the AAPS survey for high school and university students in Turkey. After the validation and administration of the Turkish version of the survey, the analysis of the data was conducted by grouping the data by grade level, school type, and gender. While there are no statistically significant differences between the averages of various groups on the survey, overall, the university students in Turkey were more expertlike than vocational high school students. On an item by item basis, there are statistically differences between the averages of the groups on many items. For example, on average, the university students demonstrated less expertlike attitudes about the role of equations and formulas in problem solving, in solving difficult problems, and in knowing when the solution is not correct, whereas they displayed more expertlike attitudes and approaches on items related to metacognition in physics problem solving. A principal component analysis on the data yields item clusters into which the student responses on various survey items can be grouped. A comparison of the responses of the Turkish and American university students enrolled in algebra-based introductory physics courses shows that on more than half of the items, the responses of these two groups were statistically significantly different, with the U.S. students on average responding to the items in a more expertlike manner.

  4. MANAGEMENT OF SIMPLE FEBRILE SEIZURES; A STUDY CONDUCTED IN IMAM HOSPITAL, OROMIEH

    N. Pashapour MD

    2009-01-01

    Full Text Available ObjectiveWhatever the health field, compliance with the recommended practice guidelines or parameters for diagnosis by specialists and expert health professionals benefits the patients. This study was conducted to determine the whether or not these guidelines or parameters are applied to the evaluation of children with first simple febrile convulsion (SFC in a regional teaching hospital.Materials & MethodsIn a prospective study conducted on children with SFC, admitted in the Pediatric ward of Imam hospital, Oromieh, records of investigations ordered between Jan 2003 and Dec 2004 for children diagnosed with SFC were collected. Practice parameters of the American Academy of Pediatrics (AAP were employed as diagnostic standards. Applied practices were compared with AAP recommended practice parameters. Investigations performed included lumbar puncture, complete blood count, CRP, ESR, blood glucose, serum calcium, electrolytes, renal function tests, urinalysis, urine culture, and blood culture, chest X-ray, EEG and CT scan.ResultsTwo hundred and fifty-one consecutive cases, aged 6-60 months, were evaluated. Complete blood count, blood glucose, serum calcium, serum electrolytes, renal function tests, urinalysis, urine culture, and blood culture were tested in all cases (100%. Lumbar puncture, chest X-ray, EEG and CT brain scan had been performed in 10%, 24%, 1.4% and 0.65% of cases, respectively. The mean number of routine investigations was twelve.ConclusionCompared to recommended practice guidelines the results of this study highlighted that children with first SFC had more often than necessary investigation. These, not only resulted in significant expense, they proved to be of little diagnostic value. Compliance with a centrally organized national program would significantly help to improve SFC evaluation.Keywords: Febrile convulsion, Simple fébrile seizure, Lumbar puncture, Convulsion, Fever

  5. Lo studio FAST: FAringotonsillite STreptococcica in età pediatrica Impatto farmacoeconomico delle Linee Guida dell’American Academy of Pediatrics sulla pratica clinica di 600 pediatri italiani

    G. L. Colombo

    2002-06-01

    Full Text Available The American Academy of Pediatrics (AAP has recently published its new guidelines for the treatment of pediatric tonsillopharyngitis; a diagnostic test for the detection of streptococci is recommended, followed by a treatment with penicillins (10 days or cephalosporins (5 days in case of positivity, and by no treatment otherwise. The success of a guideline, in general, depends on its diffusion in practice and on its adaptability to different local settings. The aim of the present study (FAST was the evaluation of the clinical and economical impact of the application of the AAP guidelines in the Italian reality. The study was conducted with the cooperation of 600 pediatricians, uniformly distributed on the Italian territory, and involved 3072 patients. The pediatricians were left free to decide whether to follow the guidelines or not. The results of the analysis indicate that only some 20% of the pediatricians adhered to the suggested protocol; the total cost (calculated in the perspective of the National Health System, with a time horizon of about 3 months per patient, nonetheless, resulted inferior in the group treated according to the proposed protocol (50,45 • vs. 53,30 •. Furthermore, a lower incidence of complications occurred in these patients. Among the “off-protocol” behaviors, two showed particularly evident misuse of health resources: inappropriate treatment after test positivity (59,15 •/patient, and treatment after test negativity (14% of the total population. Another interesting, although not surprising, result of this study was the evidence that penicillins are as effective as cephalosporins in the short-term, but significantly inferior in terms of complications, positivity for streptococci at the control test, and complications detected at follow-up.

  6. Acute hepatotoxicity: a predictive model based on focused illumina microarrays.

    Zidek, Nadine; Hellmann, Juergen; Kramer, Peter-Juergen; Hewitt, Philip G

    2007-09-01

    Drug-induced hepatotoxicity is a major issue for drug development, and toxicogenomics has the potential to predict toxicity during early toxicity screening. A bead-based Illumina oligonucleotide microarray containing 550 liver specific genes has been developed. We have established a predictive screening system for acute hepatotoxicity by analyzing differential gene expression profiles of well-known hepatotoxic and nonhepatotoxic compounds. Low and high doses of tetracycline, carbon tetrachloride (CCL4), 1-naphthylisothiocyanate (ANIT), erythromycin estolate, acetaminophen (AAP), or chloroform as hepatotoxicants, clofibrate, theophylline, naloxone, estradiol, quinidine, or dexamethasone as nonhepatotoxic compounds, were administered as a single dose to male Sprague-Dawley rats. After 6, 24, and 72 h, livers were taken for histopathological evaluation and for analysis of gene expression alterations. All hepatotoxic compounds tested generated individual gene expression profiles. Based on leave-one-out cross-validation analysis, gene expression profiling allowed the accurate discrimination of all model compounds, 24 h after high dose treatment. Even during the regeneration phase, 72 h after treatment, CCL4, ANIT, and AAP were predicted to be hepatotoxic, and only these three compounds showed histopathological changes at this time. Furthermore, we identified 64 potential marker genes responsible for class prediction, which reflected typical hepatotoxicity responses. These genes and pathways, commonly deregulated by hepatotoxicants, may be indicative of the early characterization of hepatotoxicity and possibly predictive of later hepatotoxicity onset. Two unknown test compounds were used for prevalidating the screening test system, with both being correctly predicted. We conclude that focused gene microarrays are sufficient to classify compounds with respect to toxicity prediction. PMID:17522070

  7. Experimental Study on the Ride Comfort of a Crawler Power Chassis Scale Model Based on the Similitude Theory

    ZHAO Jianzhu; WANG Fengchen; YU Bin; TONG Pengcheng; CHEN Kuifu

    2015-01-01

    The ride comfort experimental assessment of crawler off-road vehicle is relatively overlooked, and is expensive and difficult to execute with higher and higher ride comfort performance requirements. To trade off between precise and cost, an experimental method based on the similitude theory is proposed. Under the guidance of the similitude theory, a 1:5 crawler power chassis scale model equipped with a kind of variable stiffness suspension system is used. The power spectrum density(PSD), the root mean square(RMS) of weighed acceleration, peak factor, average absorbed power(AAP) and vibration dose value(VDV) are selected as ride comfort evaluation indexes, and tests results are transformed via similarity indexes to predict the performance of full-scale power chassis. PSD shows that the low-order natural frequency of the vertical natural frequency(z axis) is 1.1 Hz, and the RMS, AAP and VDV values indicate the ride comfort performance of this kind of power chassis is between the“A little uncomfortable”and“Rather uncomfortable”. From the results, low-order vertical natural frequency, obtalned by PSD, validates that the similarity relationship between two models is satisfied, and 1:5 scale model used in experiment meets the similarity relationship with the full-scale model; consequently, the ride comfort prophase evaluation with the 1:5 scale model is feasible. The attempt of applying the similitude theory to crawler vehicle ride comfort test study decreases the cost and improves the test feasibility with sufficient test precise.

  8. Synthesis and properties of double terminated acrylamide-propyl polysiloxane modified polyacrylate mini-emulsion%双端丙烯酰胺基丙基聚硅氧烷改性聚丙烯酸酯细乳液的合成及性能研究

    孙段冰; 吴明华

    2014-01-01

    A sil icone-modified polyacrylate miniemulsion was prepared by double terminated acryl⁃amide-propyl polysiloxane (diAAp-PDMS) and acrylate monomers through mini-emulsion polymerization meth⁃od. The structure of the modified polyacrylate was characterized by FTIR, the particle size of the latex and the hydrophobic properties, mechanical properties and thermal stability of the polymer were measured and com⁃pared with the unmodified polyacrylate. The results showed that the average diameter of modified emulsion was about 86 nm with narrow distribution; the hydrophobic properties, mechanical properties and thermal sta⁃bility of the modified polyacrylate film were improved.%以双端丙烯酰胺基丙基聚硅氧烷(diAAp-PDMS)为改性单体,采用细乳液聚合法改性聚丙烯酸酯乳液,采用红外表征改性聚合物的结构,测定改性乳液的粒径、胶膜的耐水性能、力学性能和耐热稳定性能,并与未改性聚丙烯酸酯乳液进行比较。结果表明:改性后乳液平均粒径在86 nm左右,分布较窄;改性胶膜的耐水性能、力学性能及耐热稳定性能均得到改善。

  9. Screening the Medicines for Malaria Venture "Malaria Box" against the Plasmodium falciparum aminopeptidases, M1, M17 and M18.

    Alessandro Paiardini

    Full Text Available Malaria is a parasitic disease that remains a global health burden. The ability of the parasite to rapidly develop resistance to therapeutics drives an urgent need for the delivery of new drugs. The Medicines for Malaria Venture have compounds known for their antimalarial activity, but not necessarily the molecular targets. In this study, we assess the ability of the "MMV 400" compounds to inhibit the activity of three metalloaminopeptidases from Plasmodium falciparum, PfA-M1, PfA-M17 and PfM18 AAP. We have developed a multiplex assay system to allow rapid primary screening of compounds against all three metalloaminopeptidases, followed by detailed analysis of promising compounds. Our results show that there were no PfM18AAP inhibitors, whereas two moderate inhibitors of the neutral aminopeptidases PfA-M1 and PfA-M17 were identified. Further investigation through structure-activity relationship studies and molecular docking suggest that these compounds are competitive inhibitors with novel binding mechanisms, acting through either non-classical zinc coordination or independently of zinc binding altogether. Although it is unlikely that inhibition of PfA-M1 and/or PfA-M17 is the primary mechanism responsible for the antiplasmodial activity reported for these compounds, their detailed characterization, as presented in this work, pave the way for their further optimization as a novel class of dual PfA-M1/PfA-M17 inhibitors utilising non-classical zinc binding groups.

  10. The Influence of Spatial Structures of Mimetic Peroxidases and Medium Microheterogenous for Enzyme Catalytic Reaction%过氧化物模拟酶空间结构及介质微环境对酶催化反应的影响

    黄应平; 罗光富; 罗勇军; 蔡汝秀

    2005-01-01

    在水溶液及胶束介质中分别研究了血红蛋白(hemoglobin, Hb),β-环糊精-氯化血红素(β-CD-hemin)和氯化血红素(hemin)作为过氧化物模拟酶对隐性亮绿(recessive brilliant green, RBG)-H2O2(或溶解氧)体系的催化特性.在Tween 80存在下,通过测定米氏常数Km和Vmax,比较了酶催化活性的大小,结果表明催化活性Hb>β-CD-hemin>hemin.分别研究了辣根过氧化物酶(horseradish peroxidase, HRP),β-CD-hemin,hemin和四羧基苯基锰卟啉(MnTCPP)对4-氨基安替比林(4-AAP)-2,3,4-三氯苯酚(2, 3, 4-trichlorophenol, TCP)体系的催化显色作用,酶及模拟酶催化活性比较为HRP>β-CD-hemin>hemin>MnTCPP.根据实验结果,讨论了胶束介质对RGB-溶解氧催化体系的作用方式以及4-AAP-TCP酶催化体系的Michaeli-Menten方程,并从不同空间结构(结合部位)模拟过氧化物酶催化性能的角度讨论了结合部位在酶催化反应中的有效作用.

  11. The phosphorus content of fluvial suspended sediment in three lowland groundwater-dominated catchments

    Ballantine, Deborah J.; Walling, Desmond E.; Collins, Adrian L.; Leeks, Graham J. L.

    2008-07-01

    SummaryThis paper reports an investigation of the phosphorus (P) content of fluvial suspended sediment samples collected from three lowland groundwater-dominated agricultural catchments in the UK. In-stream trap samplers were installed at a total of 21 locations in the catchments of the Rivers Frome and Piddle in Dorset and in the Upper Tern in Shropshire, UK. Time-integrated suspended sediment samples ( n = 187) were collected at regular intervals over a period of 22 months and analysed for total phosphorus (TP), inorganic phosphorus (IP), organic phosphorus (OP) and algal available phosphorus (AAP). TP concentrations varied between sampling sites in the Rivers Frome and Piddle, allowing key P inputs to be identified, while fractionation of P assisted in identifying the nature of these inputs. There was also significant variation in both the TP concentration and the concentration of individual fractions between the Frome and Piddle catchments and the Upper Tern. These contrasts were attributed to the differing underlying geologies, since the Frome and Piddle are underlain predominantly by chalk, whilst the Upper Tern is underlain by sandstone, and also to the different soil types present. The TP content of suspended sediment collected from the Frome catchment showed a statistically significant relationship with specific surface area, but this relationship was not found for the remaining catchments. Temporal variation in P concentrations at both the seasonal and event scale was also investigated. Seasonal variations were noted for TP concentrations and for the concentrations of IP, OP and AAP in all the study catchments, but no consistent seasonal patterns were discernible. Maximum and minimum concentrations of the individual fractions occurred during different months in each of the study catchments, suggesting that different controls operated in the individual catchments. Short-term temporal variations in TP concentrations were documented for two high flow events

  12. A clinical case study of a psychoanalytic psychotherapy monitored with functional neuroimaging

    Buchheim, Anna; Labek, Karin; Walter, Steffen; Viviani, Roberto

    2013-01-01

    This case study describes 1 year of the psychoanalytic psychotherapy using clinical data, a standardized instrument of the psychotherapeutic process (Psychotherapy process Q-Set, PQS), and functional neuroimaging (fMRI). A female dysthymic patient with narcissistic traits was assessed at monthly intervals (12 sessions). In the fMRI scans, which took place immediately after therapy hours, the patient looked at pictures of attachment-relevant scenes (from the Adult Attachment Projective Picture System, AAP) divided into two groups: those accompanied by a neutral description, and those accompanied by a description tailored to core conflicts of the patient as assessed in the AAP. Clinically, this patient presented defense mechanisms that influenced the relationship with the therapist and that was characterized by fluctuations of mood that lasted whole days, following a pattern that remained stable during the year of the study. The two modes of functioning associated with the mood shifts strongly affected the interaction with the therapist, whose quality varied accordingly (“easy” and “difficult” hours). The PQS analysis showed the association of “easy” hours with the topic of the involvement in significant relationships and of “difficult hours” with self-distancing, a defensive maneuver common in narcissistic personality structures. In the fMRI data, the modes of functioning visible in the therapy hours were significantly associated with modulation of the signal elicited by personalized attachment-related scenes in the posterior cingulate (p = 0.017 cluster-level, whole-volume corrected). This region has been associated in previous studies to self-distancing from negatively valenced pictures presented during the scan. The present study may provide evidence of the possible involvement of this brain area in spontaneously enacted self-distancing defensive strategies, which may be of relevance in resistant reactions in the course of a psychoanalytic

  13. A clinical case study of a psychoanalytic psychotherapy monitored with functional neuroimaging

    Anna eBuchheim

    2013-10-01

    Full Text Available This case study describes one year of the psychoanalytic psychotherapy using clinical data, a standardized instrument of the psychotherapeutic process (Psychotherapy process Q-Set, PQS, and functional neuroimaging (fMRI. A female dysthymic patient with narcissistic traits was assessed at monthly intervals (12 sessions. In the fMRI scans, which took place immediately after therapy hours, the patient looked at pictures of attachment-relevant scenes (from the Adult Attachment Projective Picture System, AAP divided into two groups: those accompanied by a neutral description, and those accompanied by a description tailored to core conflicts of the patient as assessed in the AAP.Clinically, this patient presented defense mechanisms that influenced the relationship with the therapist and that was characterized by fluctuations of mood that lasted whole days, following a pattern that remained stable during the year of the study. The two modes of functioning associated with the mood shifts strongly affected the interaction with the therapist, whose quality varied accordingly (‘easy’ and ‘difficult’ hours. The PQS analysis showed the association of 'easy' hours with the topic of the involvement in significant relationships and of 'difficult hours' with self-distancing, a defensive manoeuvre common in narcissistic personality structures. In the fMRI data, the modes of functioning visible in the therapy hours were significantly associated with modulation of the signal elicited by personalized attachment-related scenes in the posterior cingulate (p=0.017 cluster-level, whole-volume corrected. This region has been associated in previous studies to self-distancing from negatively valenced pictures presented during the scan.The present study may provide evidence of the possible involvement of this brain area in spontaneously enacted self-distancing defensive strategies, which may be of relevance in resistant patient reactions in the course of a

  14. A clinical case study of a psychoanalytic psychotherapy monitored with functional neuroimaging.

    Buchheim, Anna; Labek, Karin; Walter, Steffen; Viviani, Roberto

    2013-01-01

    This case study describes 1 year of the psychoanalytic psychotherapy using clinical data, a standardized instrument of the psychotherapeutic process (Psychotherapy process Q-Set, PQS), and functional neuroimaging (fMRI). A female dysthymic patient with narcissistic traits was assessed at monthly intervals (12 sessions). In the fMRI scans, which took place immediately after therapy hours, the patient looked at pictures of attachment-relevant scenes (from the Adult Attachment Projective Picture System, AAP) divided into two groups: those accompanied by a neutral description, and those accompanied by a description tailored to core conflicts of the patient as assessed in the AAP. Clinically, this patient presented defense mechanisms that influenced the relationship with the therapist and that was characterized by fluctuations of mood that lasted whole days, following a pattern that remained stable during the year of the study. The two modes of functioning associated with the mood shifts strongly affected the interaction with the therapist, whose quality varied accordingly ("easy" and "difficult" hours). The PQS analysis showed the association of "easy" hours with the topic of the involvement in significant relationships and of "difficult hours" with self-distancing, a defensive maneuver common in narcissistic personality structures. In the fMRI data, the modes of functioning visible in the therapy hours were significantly associated with modulation of the signal elicited by personalized attachment-related scenes in the posterior cingulate (p = 0.017 cluster-level, whole-volume corrected). This region has been associated in previous studies to self-distancing from negatively valenced pictures presented during the scan. The present study may provide evidence of the possible involvement of this brain area in spontaneously enacted self-distancing defensive strategies, which may be of relevance in resistant reactions in the course of a psychoanalytic psychotherapy. PMID

  15. Plasma 25-hydroxyvitamin D concentrations and periodontal disease in postmenopausal women

    Millen, Amy E.; Hovey, Kathleen M.; LaMonte, Michael J.; Swanson, Mya; Andrews, Christopher A.; Kluczynski, Melissa A.; Genco, Robert J.; Wactawski-Wende, Jean

    2013-01-01

    Background Vitamin D has anti-inflammatory and anti-microbial properties that, together with its influence on bone health, may confer periodontal benefit. Methods We investigated cross-sectional associations (1997–2000) between plasma 25-hydroxyvitamin D concentrations [25(OH)D] and periodontal measure among 920 postmenopausal women. Chronic measures of disease were defined based on: 1) alveolar crestal height (ACH) measures from intraoral radiographs and tooth loss, and the 2) Center for Disease Control and Prevention (CDC)/American Academy of Periodontology (AAP) criteria using measures of clinical attachment level (CAL) and probing pocket depth (PD). Acute oral inflammation was assessed by the % of gingival sites that bled upon assessment with a probe. Logistic regression was used to estimate the odds ratios (OR) and 95% confidence intervals (CIs) for periodontal disease among participants with adequate ([25(OH)D]≥50 nmol/L) compared to deficient/inadequate ([25(OH)D]<50 nmol/L) vitamin D status adjusted for age, dental visit frequency, and body mass index. Results No association was observed between vitamin D status and periodontal disease defined by ACH and tooth loss (adjusted OR=0.96, 95% CI: 0.68–1.35). In contrast, women with adequate compared to deficient/inadequate vitamin D status had a 33% lower odds (95% CI: 5%–53%) of periodontal disease defined using the CDC/AAP definition and a 42% lower odds (95% CI: 21%-58%) of having ≥50% of gingival sites that bled. Conclusion Vitamin D status was inversely associated with gingival bleeding, an acute measure of oral health and inflammation and inversely associated with clinical categories of chronic periodontal disease that incorporated PD, an indicator of oral inflammation. However, vitamin D was not associated with chronic periodontal disease based on measures of ACH in combination with tooth loss. PMID:23259413

  16. 113例小儿骶尾部畸胎瘤的病理探讨%Sacrocoecygeal Teratomas in Infants and Children A Clinical and Pathological Study of 110 Cases

    张顺利; 于佩良; 乔柏生

    1989-01-01

    本文报道113例小儿骶尾部畸胎瘤的回顾性研究,强调组织学类型与临床有关参数及预后的联系,提出新生儿或婴儿Ⅱ级以内未成熟畸胎瘤一般应视为良性,伴有先天性畸形惠儿的肿瘤一般为良性.骶尾部畸胎瘤可能有两种演变类型.%The histopathological study of 113 cases of sacrococcygeal teratoma in infants and children shows the correlation between the histological typing and the clinical course.A tumor was detected within 3 months after their birth,and only 3.6% were malignant.Of those discovered at the age of 3 months or beyond.68.1% were malignant.Teratomas of 18 inrants (16%)associated with other anomalies were benign,wherens,in 95 children without associated anomalies 23% were malignant.Mature teratomas (75.5%) was found to be of AAP Type Ⅰ-Ⅳ.Total exsecsion was performed in two-thirds of them with a mortality of 10%.Immature te ratoma (8%) usually appeared externally (AAP Type Ⅰ).Postoperatirely.all five chiidren were living and well in a follow-up 9f 4 years,Most of malignant teratoma(19.5%) belonged to TypeⅢ and Type Ⅳ,and 20 of 22malignancies were yolk sac tumors.Follow up was carried out in 10 of them and all died on an average 4.5 months postoperatively.Immature teratomas in newborns and infants should always be considered as benign clinically and complete excision would be sufficient,but foliow up is still necessary.

  17. Myocardial impulse propagation is impaired in right ventricular tissue of Zucker Diabetic Fatty (ZDF rats

    Olsen Kristine Boisen

    2013-01-01

    Full Text Available Abstract Background Diabetes increases the risk of cardiovascular complications including arrhythmias, but the underlying mechanisms remain to be established. Decreased conduction velocity (CV, which is an independent risk factor for re-entry arrhythmias, is present in models with streptozotocin (STZ induced type 1 diabetes. Whether CV is also disturbed in models of type 2 diabetes is currently unknown. Methods We used Zucker Diabetic Fatty (ZDF rats, as a model of type 2 diabetes, and their lean controls Zucker Diabetic Lean (ZDL rats to investigate CV and its response to the anti-arrhythmic peptide analogue AAP10. Gap junction remodeling was examined by immunofluorescence and western blotting. Cardiac histomorphometry was examined by Masson`s Trichrome staining and intracellular lipid accumulation was analyzed by Bodipy staining. Results CV was significantly slower in ZDF rats (56±1.9 cm/s compared to non-diabetic controls (ZDL, 66±1.6 cm/s, but AAP10 did not affect CV in either group. The total amount of Connexin43 (C×43 was identical between ZDF and ZDL rats, but the amount of lateralized C×43 was significantly increased in ZDF rats (42±12 % compared to ZDL rats (30±8%, p Conclusion CV is reduced in type 2 diabetic ZDF rats. The CV disturbance may be partly explained by increased lateralization of C×43, but other factors are likely also involved. Our data indicates that lipotoxicity potentially may play a role in development of conduction disturbances and arrhythmias in type 2 diabetes.

  18. A response to Mimics of child abuse: Can choking explain abusive head trauma? [35 (2015) 33-37].

    Galaznik, John G

    2016-04-01

    In the recently published article in this journal, "Mimics of Child Abuse: Can Choking Explain Abusive Head Trauma?",(1) the author chose to revisit a discussion prompted by a case report from 5 years ago which was inappropriate in his opinion. He went further to suggest that bringing an unvalidated mechanism of injury into the legal setting "obstructs justice", is a "further victimization of the child", and is a "travesty of justice".(1) Given the "Shaken Baby Syndrome: Rotational Cranial Injuries" has always been only an unvalidated hypothesis lacking experimental confirmation, the exploring of alternative injury mechanisms should be entirely appropriate. In 2010, the post publication discussion ended with a challenge to the American Academy of Pediatrics Committee on Child Abuse and Neglect (AAP COCAN) to either support the pure shaking mechanism with quality EBMS or eliminate any positive support for it from any official policy statement until the exact nature of each injury that pure abusive shaking has the potential to cause is clearly defined and supported with quality experimental research.(4) Since this is an area of acknowledged controversy by the AAP, it is appropriate to examine the evidence based experimental literature that has emerged over the last five years that is relevant to the abusive shaking hypothesis and the hypothesis of any primary brain-lethal hypoxic event leading to the findings of retinal hemorrhages, extra-axial bleeding, and brain injury when an infant presents to medical attention after an Acute/Apparent Life Threatening Event. In that light, this review was undertaken. PMID:26828828

  19. The Circle Of Security Parenting and parental conflict: a single case study

    ChiaraPazzagli

    2014-08-01

    Full Text Available The Circle of Security Parenting (COS-P is an early attachment based intervention that can be used with groups, dyads, and individuals. Created in the USA and now used in many countries, COS-P is a visually based approach that demonstrates its central principles through videos of parent/child interactions. The core purpose of the COS-P is to provide an opportunity for caregivers to reflect on their child’s needs and on the challenges each parent faces in meeting those needs. Even though there is a wide range of clinical settings in which child/parent attachment is an important component of assessment there is limited empirical data on when and how attachment based interventions are appropriate for specific clinical profiles and contexts. The aim of this paper is to present a clinical application of COS-P in order to explore and reflect on some specific therapeutic tasks where it works and on some clinical indicators and contexts appropriate for its application. A single case study of a father, “M.” (43 yrs. old in conflict for the custody of his 5yrs old daughter is reported. The Adult Attachment Projective Picture System (AAP, the Parenting Stress Index, the Strengths and Difficulties Questionnaire, and the Parental Alliance Measure, were administered pre- and post-intervention. The clinical significance analysis method revealed that numerous changes occurred in the father. The AAP showed improvements in the level of agency of self. M. made gains in his capacity to use internal resources and to increase his agency of self. M. was classified as recovered in his perception of the child’s functioning and as improved in his parenting stress and parenting alliance with the mother. Considerations on specific contexts and clinical indicators for the application of COS-P are proposed.

  20. Inmunodetección de metaloproteinasas de matriz extracelular (MMPs-2, -9, -13 y -14 en lesiones apicales asociadas con periodontitis apical asintomática Immunodetection of matrix metalloproteinases (MMPs-2, -9, -13 and -14 in periapical lesions associated with asymptomatic apical periodontitis

    V Mundi Burgos

    2011-04-01

    Full Text Available La periodontitis apical asintomática (PAa es una patología infecciosa caracterizada por destrucción ósea perirradicular asociada a un proceso inflamatorio crónico y producción de mediadores inflamatorios, entre los cuales se encuentran las metaloproteinasas de matriz extracelular (MMPs. Entre éstas, las MMPs-13, -14, -2 y -9, son producidas por el tejido óseo y degradan sinérgicamente el colágeno tipo I, principal componente de los tejidos periodontales, y gelatina, producto de la degradación y desnaturación del colágeno. El objetivo de este estudio fue determinar el patrón de expresión de las MMPs-2, -9, -13 y -14 en granulomas periapicales (GPAs, quistes radiculares inflamatorios (QRIs y ligamento periodontal sano (LS. Materiales y Métodos: Se seleccionaron 12 pacientes con diagnóstico clínico de PAa e indicación de exodoncia a partir de los cuales se obtuvieron biopsias de lesiones periapicales (LPAs. Como controles, se seleccionaron 7 individuos con indicación de exodoncia de premolares por ortodoncia, obteniéndose biopsias de LS. Se efectuó el diagnóstico anátomo-patológico de los especímenes y se caracterizó la expresión de las MMPs en estudio mediante inmunohistoquímica. Resultados: Las MMPs en estudio sólo se detectaron en GPAs y QRIs, y se inmunolocalizaron principalmente en el infiltrado inflamatorio de éstos. Adicionalmente, la MMP-2 se identificó en fibroblastos del tejido conectivo. Conclusiones: MMPs-2, -9, -13 y -14 se expresan predominantemente en el infiltrado inflamatorio de las LPAs y no en LS, y por tanto se sugiere la participación de estos mediadores en la patogénesis de la PAa.Asymptomatic apical periodontitis (aAP is an infectious disease characterized by perirradicular bone destruction associated with chronic inflammation and release of inflammatory mediators, such as matrix metalloproteinases (MMPs. MMPs-13, -14 and -2, -9 are bone-expressed enzymes that can synergistically degrade

  1. Acometimento pulmonar na doença de Behçet: uma boa experiência com o uso de imunossupressores Pulmonary involvement in Behcet's disease: a positive single-center experience with the use of immunosuppressive therapy

    Alfredo Nicodemos Cruz Santana

    2008-06-01

    Full Text Available OBJETIVO: A doença de Behçet (DB representa uma patologia sistêmica, cuja sobrevida se relaciona com a presença de acometimento pulmonar. Entretanto, sugere-se que pacientes com diferentes tratamentos podem apresentar diferentes prognósticos. O objetivo deste estudo foi avaliar a evolução clínica e tomográfica, bem como a sobrevida deste pacientes com acometimento pulmonar relacionado à DB acompanhados em nosso serviço. MÉTODOS: Uma análise retrospectiva de nossa experiência com pacientes com acometimento pulmonar relaionado a DB acompanhados de 1 de Janeiro de 1988 a 30 de Abril de 2006. Os dados clínicos, radiológicos, terapêuticos e de sobrevida foram obtidos dos prontuários médicos. RESULTADOS: Foram identificados 9 pacientes, com idade média de 34 ± 11,5 anos, sendo 7 deles do sexo masculino. Os achados radiológicos foram aneurisma de artéria pulmonar (AAP em 8 pacientes, embolia pulmonar em 3 (resultando em uma incidencia de 5,11 casos/100 paciente-anos, hemorragia alveolar em 1 e hipertensão pulmonar em 1 de 9 doentes. O tratamento consistiu-se de prednisona mais clorambucil (ou ciclofosfamida ou micofenolato de mofetil em todos os 9 pacientes, com resolução total ou parcial dos AAP. O paciente com AAP e hipertensão pulmonar também recebeu sildenafil e warfarina, com boa resposta clínica e tomográfica. A sobrevida de nossos pacientes foi de 88,8% em 3 e 5 anos, com acompanhamento médio de 6,52 anos. CONCLUSÕES: Pacientes com acometimento pulmonar relacionado à DB podem apresentar boa sobrevida com tratamento imunossupressor, e a DB deve ser lembrada como uma possível causa de hipertensão pulmonar e hemorragia alveolar.OBJECTIVE: Behcet's syndrome, or Behcet's disease (BD, is a multisystem pathology, and survival is related to pulmonary involvement. However, it appears that different treatments correlate with different prognoses. The aim of this study was to evaluate clinical and tomographic evolution, as

  2. Biofilm synthesis and its relationship with genetic characteristics in clinical methicillin-resistant staphylococci

    Nikolaos Giormezis

    2015-09-01

    Full Text Available Staphylococcus aureus can cause a broad range of infections, including skin infections, pneumonia and bacteraemia. Coagulase-negative staphylococci (CNS, mainly S. epidermidis, have also emerged as important pathogens, especially in immunocompromised patients or those with prosthetic devices, such as intravascular catheters or biomaterials. Of great importance in the initiation of these infections is the ability of staphylococci to adhere to various surfaces, such as host tissues and prosthetic devices and to form biofilm. The staphylococcal adhesins are encoded by a number of genes such as fnbA (S. aureus fibronectin binding protein A, sasG (S. aureus surface protein G, aap (S. epidermidis accumulation associated protein, bhp (Bap homologue protein and fbe (fibrinogen binding protein epidermidis. In this study, 106 methicillin-resistant S. aureus (MRSA, 145 methicillin-resistant S. epidermidis (MRSE and 70 non-epidermidis methicillin-resistant CNS (MR-CNS; 58 S. haemolyticus, 10 S. hominis and two S. lugdunensis were compared in terms of biofilm formation, antimicrobial resistance, clonal distribution and adhesin genes carriage. Isolates were classified into pulsotypes by PFGE and assigned to sequence types by MLST. In total, 121/321 isolates (37.7% produced biofilm and 219 (68.2% carried ica operon. The majority was multidrug resistant (94.7% and carried one or more adhesin genes. MRSE and all other MR-CNS prevailed in biofilm formation (P < 0.001 and antimicrobial resistance (P < 0.05 as compared to MRSA. MRSE also prevailed in ica carriage compared to the other methicillin-resistant staphylococci (P ≤ 0.007 Among MRSE, isolates from bacteraemias prevailed in biofilm formation (P = 0.031, whereas, strains from prosthetic device-associated infections carried more frequently aap (P = 0.003. Even though PFGE showed genetic diversity among MRSE, MLST revealed three major clones (ST2, ST5, ST16. MRSA isolates were less diverse, with five PFGE

  3. Evaluación del screening de perfil lipídico propuesto por la Academia Americana de Pediatría Usefulness of the American Academy of Pediatrics lipid screening

    Kim-Lan Alliu M

    2012-06-01

    Full Text Available Objetivo: Determinar si los criterios de screening adoptados por la American Academy of Pediatrics para pesquisar alteraciones de perfil lipídico son útiles en población consultante del Centro Asistencial Norte de Antofagasta. Pacientes y Método: 61 pacientes entre 2 y 14 años fueron sometidos a screening de perfil lipídico (historia familiar de enfermedad cardiovascular, hipercolesterolemia parental, sobrepeso, hipertensión arterial, tabaquismo, consumo elevado de grasas para analizar si se relacionaban con los resultados obtenidos del laboratorio. El diagnóstico se realizó considerando los valores de colesterol total, HDL, LDL y triglicéridos. El diseño correspondió a un estudio analítico de corte transversal. Para analizar los grupos se utilizó el /-test y la prueba de Fisher con α = 0,05. Resultados: Sólo hubo diferencia significativa en el antecedente de hipercolesterolemia parental (p = 0,013 con sensibilidad del 31% y especificidad de 63%, observándose un riesgo de 6 veces más posibilidades de tener dislipidemia en los hijos de padres con hipercolesterolemia (OR con intervalo de confianza al 95% de 6,75 (1,18 - 68,51. Conclusión: En la muestra, según los criterios de screening, sólo el antecedente de hipercolesterolemia de los padres resultó valioso para determinar si los pacientes tenían más riesgo de presentar dislipidemia.Objective: To determine if the screening adopted by the American Academy of Pediatrics to search for alterations in lipid profile is useful in the population of Centro Asistencial Norte of Antofagasta. Patients and Method: 61 patients 2 to 14 years of age were screened using the AAP lipid profile (family history of cardiovascular disease, hypercholesterolemia of parents, presence of overweight, arterial hypertension, tobacco addiction, consumption of fats to evaluate its relationship to laboratory results (total cholesterol, HDL, LDL and triglycerides. Ain analytical study of cross section was

  4. An assessment on epitope prediction methods for protozoa genomes

    Resende Daniela M

    2012-11-01

    Full Text Available Abstract Background Epitope prediction using computational methods represents one of the most promising approaches to vaccine development. Reduction of time, cost, and the availability of completely sequenced genomes are key points and highly motivating regarding the use of reverse vaccinology. Parasites of genus Leishmania are widely spread and they are the etiologic agents of leishmaniasis. Currently, there is no efficient vaccine against this pathogen and the drug treatment is highly toxic. The lack of sufficiently large datasets of experimentally validated parasites epitopes represents a serious limitation, especially for trypanomatids genomes. In this work we highlight the predictive performances of several algorithms that were evaluated through the development of a MySQL database built with the purpose of: a evaluating individual algorithms prediction performances and their combination for CD8+ T cell epitopes, B-cell epitopes and subcellular localization by means of AUC (Area Under Curve performance and a threshold dependent method that employs a confusion matrix; b integrating data from experimentally validated and in silico predicted epitopes; and c integrating the subcellular localization predictions and experimental data. NetCTL, NetMHC, BepiPred, BCPred12, and AAP12 algorithms were used for in silico epitope prediction and WoLF PSORT, Sigcleave and TargetP for in silico subcellular localization prediction against trypanosomatid genomes. Results A database-driven epitope prediction method was developed with built-in functions that were capable of: a removing experimental data redundancy; b parsing algorithms predictions and storage experimental validated and predict data; and c evaluating algorithm performances. Results show that a better performance is achieved when the combined prediction is considered. This is particularly true for B cell epitope predictors, where the combined prediction of AAP12 and BCPred12 reached an AUC value

  5. Assessment of Pain Management in Pediatric Emergency Department in Mashhad -Iran

    Ahmadshah Farhat

    2013-12-01

    Full Text Available Introduction: Pain may be described as a sensation of hurt or strong discomfort and is the body's way of sending message to the brain that an injury has occurred. Pain medicines block these messages or reduce their effect on the brain. Accurate administration of analgesia have a long –lasting effect on children whole experience of medical care and affects parents' and children's future reaction to pediatrics emergency departments. The purpose of this study was to evaluate pain management on children in our emergency department. Materials and Methods: In this study we evaluated the relief of pain and anxiety on 100 children who referred to our pediatric Emergency Department (ED in Imam Reza Hospital- Mashhad .The patients were assessed based on the American Academy of Pediatrics (AAP recommendations about pain.  Results: Patients were gone under IV Line 97%, Intubation 5% and Lumbar Puncture 28%. Training had been provided to 70% participants in the Emergency Department. Nonpharmacologic stress reduction was used in 35% of cases. Family presence was allowed only in 5%. Prehospital pain controlling was began on 20% of patients and continued in ED on 40%. At the time of discharge 40% prescribed analgesics. Sedation and pain prophylaxis was provided for 10% of patients undergoing painful procedures in ED.  Conclusion: According to results, pain management in our Pediatric Emergency Department was inadequate. Physicians and prehospital EMS providers should be justified about the importance of pain relieving and trained how to use all available analgesic and sedative options.

  6. The Antioxidant Profiles, Lysosomal and Membrane Enzymes Activity in Patients with Acute Pancreatitis

    Halina Milnerowicz

    2014-01-01

    Full Text Available Oxidative stress and inflammatory mediators, such as IL-6, play an important role in the pathophysiology of acute pancreatitis. The study was aimed to assess the degree of the pro/antioxidative imbalance and estimate which antioxidant plays a role in the maintenance of pro/antioxidative balance during acute pancreatitis. The study was investigated in the blood of 32 patients with acute pancreatitis and 37 healthy subjects. IL-6 concentration as early marker of inflammation was determinated. The intensity of oxidative stress was assessed by TBARS concentration. To investigate antioxidative status, the GPx and Cu/Zn SOD activities and the levels of GSH, MT, SH groups, and TRAP were measured. The concentrations of Cu and Zn as ions participating in the maintenance of antioxidant enzymes stability and playing a role in the course of disease were determinated. The activities of GGT, AAP, NAG, and β-GD as markers of tissue damage were also measured. An increase in IL-6 concentration, which correlated with Ranson criteria, and an increase in GPx activity, levels of MT, TBARS, or GGT, and NAG activities in patients group compared to healthy subjects were demonstrated. A decrease in GSH level in patients group compared to control group was noted. The studies suggest that GPx/GSH and MT play the role of the first line of defence against oxidative stress and pro/antioxidant imbalance in the course of acute pancreatitis.

  7. Comparative evaluation of CO2 and iopromide as contrast agents for renal angiography in dogs

    The aim of this study was to compare the renal injury and the quality of images of iodinated contrast (iopromide) and CO2 using digital subtraction angiography in dogs. 17 dogs were randomly assigned to receive iodinated contrast (iopromide, n=5) or CO2 (n=12) using 6F pigtail catheter, which was introduced via femoral approach to perform aortogram. Serum creatinine (S.Cr.) and urinary enzymes such as N-acetyl-â-Dglucosaminidase (NAG), alanine aminopeptidase (AAP), gamma glutamyl transferase (GGT) were measured before and 0 and 48 hr after DSA to assess renal injury. PaCO2 was measured before and immediately after the procedure. There was no change in S.Cr. in both groups; however, significantly more enzymuria was seen in iodinated contrast. Images obtained with iodinated contrast agents were superior to those with CO2. However, CO2 is good alternative contrast agent in high risk individuals to reduce the risk of renal injury and quality of images is able to delineate the aorta and main renal artery

  8. A literature review of the relationship between breastfeeding and early childhood caries

    Narjes Amiri TehraniZadeh

    2012-01-01

    Full Text Available Background and Aims: Improper feeding is one of the most important etiologic factors in early childhood caries (ECC. In some clinical trials, breast feeding (BF is mentioned as a causing factor in ECC. Results in this topic are different or even controversial. It is vital that all general advices given out are consistent with general health education messages. The aim of this study was to conduct a systematic review on the relationship between breast feeding and early childhood caries. Materials and Methods: Articles, guidelines, related books, and specific databases such as SID, PubMed, Scirus, Elsevier, American Association of Pediatric Dentistry (AAPD, American Association of Pediatrics (AAP and American Dental Association (ADA were searched for related topics cited since 1990. Early childhood caries definition, breast feeding and other variables such as sample size, study location and study design were analyzed.Results: According to data analysis, there was a lack of methodological consistency and inconsistent definitions of ECC and BF in the literature making it difficult to draw conclusions. Results about relationship between prolonged BF and ECC were inconclusive. There was a positive relationship between nocturnal BF and ECC. A history of BF did not have any effect on the caries incidence.Conclusion: Because of the role of breast feeding in children's health, it is important to inform the parents about the possibility of dental caries due to nocturnal breast feeding.

  9. Coherent measures of the impact of co-authors in peer review journals and in proceedings publications

    Ausloos, Marcel

    2015-01-01

    This paper focuses on the coauthor effect in different types of publications, usually not equally respected in measuring research impact. {\\it A priori} unexpected relationships are found between the total coauthor core value, $m_a$, of a leading investigator (LI), and the related values for their publications in either peer review journals ($j$) or in proceedings ($p$). A surprisingly linear relationship is found: $ m_a^{(j)} + 0.4\\;m_a^{(p)} = m_a^{(jp)} $. Furthermore, another relationship is found concerning the measure of the total number of citations, $A_a$, i.e. the surface of the citation size-rank histogram up to $m_a$. Another linear relationship exists : $A_a^{(j)} + 1.36\\; A_a^{(p)} = A_a^{(jp)} $. These empirical findings coefficients (0.4 and 1.36) are supported by considerations based on an empirical power law found between the number of joint publications of an author and the rank of a coauthor. Moreover, a simple power law relationship is found between $m_a$ and the number ($r_M$) of coauthor...

  10. Antibiotic Stewardship: Reassessment of Guidelines for Management of Neonatal Sepsis

    Cotten, C. Michael

    2016-01-01

    Since publication of the first Guidelines to Prevent Perinatal Group B Strep (GBS) disease in 1996, the incidence and mortality from early onset sepsis (EOS), and particularly GBS, the leading cause of EOS, has drastically decreased. In 2010, the Centers for Disease Control (CDC) provided updated Guidelines for the prevention of perinatal Group B streptococcal disease. In 2012, the AAP Committee on Fetus and Newborn (COFN) provided a Clinical Report that provided a thorough review of EOS and voiced overall support of the 2010 CDC Guidelines. In addition, the COFN authors suggested an approach different from the 2010 CDC Guidelines for at-risk asymptomatic infants. The COFN also ventured into the uncertain territory of recommending longer duration of empirical antibiotic treatment for asymptomatic infants with negative cultures, but abnormal CBC and/or CRP values. With the current focus on antibiotic stewardship, the 2012 COFN Clinical Report algorithms evoked questions from the Neonatology community. The COFN has recently responded with modified recommendations for empirical antibiotic duration and explanations for the recommendations for use of antibiotics in subgroups of infants for whom the CDC did not recommend starting antibiotics. Our goal in this article is to review the 2010 CDC and 2012 COFN guidelines and COFN's recently published guideline modifications and discuss mechanisms that may reduce the number of term and near term infants to be started on antibiotics. PMID:25678005

  11. Quantification of histone modifications by parallel-reaction monitoring: a method validation.

    Sowers, James L; Mirfattah, Barsam; Xu, Pei; Tang, Hui; Park, In Young; Walker, Cheryl; Wu, Ping; Laezza, Fernanda; Sowers, Lawrence C; Zhang, Kangling

    2015-10-01

    Abnormal epigenetic reprogramming is one of the major causes leading to irregular gene expression and regulatory pathway perturbations, in the cells, resulting in unhealthy cell development or diseases. Accurate measurements of these changes of epigenetic modifications, especially the complex histone modifications, are very important, and the methods for these measurements are not trivial. By following our previous introduction of PRM to targeting histone modifications (Tang, H.; Fang, H.; Yin, E.; Brasier, A. R.; Sowers, L. C.; Zhang, K. Multiplexed parallel reaction monitoring targeting histone modifications on the QExactive mass spectrometer. Anal. Chem. 2014, 86 (11), 5526-34), herein we validated this method by varying the protein/trypsin ratios via serial dilutions. Our data demonstrated that PRM with SILAC histones as the internal standards allowed reproducible measurements of histone H3/H4 acetylation and methylation in the samples whose histone contents differ at least one-order of magnitude. The method was further validated by histones isolated from histone H3 K36 trimethyltransferase SETD2 knockout mouse embryonic fibroblasts (MEF) cells. Furthermore, histone acetylation and methylation in human neural stem cells (hNSC) treated with ascorbic acid phosphate (AAP) were measured by this method, revealing that H3 K36 trimethylation was significantly down-regulated by 6 days of treatment with vitamin C. PMID:26356480

  12. 微乳液/离子液体协同增敏分光光度法测定水中苯酚%Determination of Phenol by Synergism Sensitizing of Triton X-1O0 Microemulsion/Ionic Liquids Spectroscopic Method

    姜蓉蓉; 朱霞石

    2011-01-01

    @@ 1引言 人体摄入含有一定量苯酚的饮用水后,会出现急性中毒症状,可引起头疼、贫血及神经系统障碍.目前,测定苯酚的方法有电化学法[1]、色谱法[2]和光谱法等.国家规定的环境监测分析方法是4-氨基安替比林(4-AAP)光度法,基于K3Fe(CN)6氧化苯酚生成的氧化物与4-AAP络合显色[3].但是此法测定灵敏度不高.%The sensitizing effect of Triton X-100 microemulsion/ionic liquids (ILs) on the determination of phenol by spectroscopic method was developed. The sensitizing mechanism and various factors influencing adsorption spectra were discussed. Under the optimum conditions, the linear equation was A=0.1986c (mg/L)- 0.023 (correlation coefficient r=0.9988). The linear range and detection limit were 0.12-20. 00 mg/L and 12.0 μg/L, respectively. The proposed method was applied to the determination of phenol in water samples with satisfactory results.

  13. New copper(II) complexes with dopamine hydrochloride and vanillymandelic acid: Spectroscopic and thermal characterization

    Mohamed, Gehad G.; Nour El-Dien, F. A.; El-Nahas, R. G.

    2011-10-01

    The dopamine derivatives participate in the regulation of wide variety of physiological functions in the human body and in medication life. Increase and/or decrease in the concentration of dopamine in human body reflect an indication for diseases such as Schizophrenia and/or Parkinson diseases. The Cu(II) chelates with coupled products of dopamine hydrochloride (DO.HCl) and vanillymandelic acid (VMA) with 4-aminoantipyrine (4-AAP) are prepared and characterized. Different physico-chemical techniques namely IR, magnetic and UV-vis spectra are used to investigate the structure of these chelates. Cu(II) forms 1:1 (Cu:DO) and 1:2 (Cu:VMA) chelates. DO behave as a uninegative tridentate ligand in binding to the Cu(II) ion while VMA behaves as a uninegative bidentate ligand. IR spectra show that the DO is coordinated to the Cu(II) ion in a tridentate manner with ONO donor sites of the phenolic- OH, -NH and carbonyl- O, while VMA is coordinated with OO donor sites of the phenolic- OH and -NH. Magnetic moment measurements reveal the presence of Cu(II) chelates in octahedral and square planar geometries with DO and VMA, respectively. The thermal decomposition of Cu(II) complexes is studied using thermogravimetric (TG) and differential thermal analysis (DTA) techniques. The activation thermodynamic parameters, such as, energy of activation, enthalpy, entropy and free energy change of the complexes are evaluated and the relative thermal stability of the complexes are discussed.

  14. A child's nightmare. Mum comes and comforts her child. Attachment evaluation as a guide in the assessment and treatment in a clinical case study

    Silvia eSalcuni

    2014-08-01

    Full Text Available There is a gap between proposed theoretical attachment theory frameworks, measures of attachment in the assessment phase and their relationship with changes in outcome after a psychodynamic oriented psychotherapy. Based on a clinical case study of a young woman with Panic Attack Disorder, this paper examined psychotherapy outcome findings comparing initial and post-treatment assessments, according to the mental functioning in S and M-axis of the Psychodynamic Diagnostic Manual (PDM. Treatment planning and post-treatment changes were described with the main aim to illustrate from a clinical point of view why a psycho-dynamic approach, with specific attention to an attachment theory stance, was considered the treatment of choice for this patient. The Symptom Check List 90 Revised (SCL-90-R and the Shedler-Westen Assessment Procedure (SWAP–200 were administered to detect patient’s symptomatic perception and clinician’s diagnostic points of view, respectively; the Adult Attachment Interview (AAI and the Adult Attachment Projective Picture System (AAP were also administered as to pay attention to patient’s unconscious internal organization and changes in defense processes. A qualitative description of how the treatment unfolded was included. Findings highlight the important contribution of attachment theory in a 22-month psychodynamic psychotherapy framework, promoting resolution of patient’s symptoms and adjustment.

  15. "A child's nightmare. Mum comes and comforts her child." Attachment evaluation as a guide in the assessment and treatment in a clinical case study.

    Salcuni, Silvia; Di Riso, Daniela; Lis, Adriana

    2014-01-01

    There is a gap between proposed theoretical attachment theory frameworks, measures of attachment in the assessment phase and their relationship with changes in outcome after a psychodynamic oriented psychotherapy. Based on a clinical case study of a young woman with Panic Attack Disorder, this paper examined psychotherapy outcome findings comparing initial and post-treatment assessments, according to the mental functioning in S and M-axis of the psychodynamic diagnostic manual. Treatment planning and post-treatment changes were described with the main aim to illustrate from a clinical point of view why a psycho-dynamic approach, with specific attention to an "attachment theory stance," was considered the treatment of choice for this patient. The Symptom Check List 90 Revised (SCL-90-R) and the Shedler-Westen Assessment Procedure (SWAP-200) were administered to detect patient's symptomatic perception and clinician's diagnostic points of view, respectively; the Adult Attachment Interview and the Adult Attachment Projective Picture System (AAP) were also administered as to pay attention to patient's unconscious internal organization and changes in defense processes. A qualitative description of how the treatment unfolded was included. Findings highlight the important contribution of attachment theory in a 22-month psychodynamic psychotherapy framework, promoting resolution of patient's symptoms and adjustment. PMID:25191293

  16. Defining Patient Centric Pharmaceutical Drug Product Design.

    Stegemann, Sven; Ternik, Robert L; Onder, Graziano; Khan, Mansoor A; van Riet-Nales, Diana A

    2016-09-01

    The term "patient centered," "patient centric," or "patient centricity" is increasingly used in the scientific literature in a wide variety of contexts. Generally, patient centric medicines are recognized as an essential contributor to healthy aging and the overall patient's quality of life and life expectancy. Besides the selection of the appropriate type of drug substance and strength for a particular indication in a particular patient, due attention must be paid that the pharmaceutical drug product design is also adequately addressing the particular patient's needs, i.e., assuring adequate patient adherence and the anticipate drug safety and effectiveness. Relevant pharmaceutical design aspects may e.g., involve the selection of the route of administration, the tablet size and shape, the ease of opening the package, the ability to read the user instruction, or the ability to follow the recommended (in-use) storage conditions. Currently, a harmonized definition on patient centric drug development/design has not yet been established. To stimulate scientific research and discussions and the consistent interpretation of test results, it is essential that such a definition is established. We have developed a first draft definition through various rounds of discussions within an interdisciplinary AAPS focus group of experts. This publication summarizes the outcomes and is intended to stimulate further discussions with all stakeholders towards a common definition of patient centric pharmaceutical drug product design that is useable across all disciplines involved. PMID:27317470

  17. The role of both parents’ attachment pattern in understanding childhood obesity

    ClaudiaMazzeschi

    2014-07-01

    Full Text Available Within the research area on the determinants of childhood obesity, a relatively new approach is the use of attachment theory to explore the mechanisms underlying children’s obesity risk, especially considered as emotion regulation strategies in parent-child relationship. Few are the empirical researches that have addressed this issue. The empirical investigations have used self-report measures to assess adult attachment. In attachment studies, the use of interview methods and/or performance-based instruments is advised to evaluate the entire range of possible adult attachment patterns and comprehensively explain the emotional strategies, correlates and consequences of individual differences in attachment system functioning. The aim of this study was to explore the extent to which both parents’ attachment patterns serve as self-regulative mechanisms related to childhood overweight/obesity by the Adult Attachment Projective Picture System (AAP in a sample of 44 mothers and fathers of children referred for obesity. Insecure attachment was found as a risk factor both for mothers’ and fathers’. Also unresolved/disorganization was found to play a significant role in childhood obesity. The role of father’s attachment was explored and findings suggested considering it in etiology and treatment of childhood obesity.

  18. Chapter Oral Health Advocates: A Nationwide Model for Pediatrician Peer Education and Advocacy about Oral Health.

    Lewis, Charlotte W; Barone, Lauren; Quinonez, Rocio B; Boulter, Suzanne; Mouradian, Wendy E

    2013-01-01

    Objective. (1) To describe an innovative program training US pediatricians to be Chapter Oral Health Advocates (COHAs). (2) To provide insight into COHAs' experiences disseminating oral health knowledge to fellow pediatricians. Patients and Methods. Interviews with 40 COHAs who responded to an email request, from a total of 64 (62% response). Transcripts were analyzed for common themes about COHA activities, facilitators, and barriers. Results. COHAs reported positive experiences at the AAP oral health training program. A subset of academic COHAs focused on legislative activity and another on resident education about oral health. Residents had an easier time adopting oral health activities while practicing pediatricians cited time constraints. COHAs provided insights into policy, barriers, and facilitators for incorporating oral health into practice. Conclusions. This report identifies factors influencing pediatricians' adoption of oral health care into practice. COHAs reported successes in training peers on integrating oral health into pediatric practice, identified opportunities and challenges to oral health implementation in primary care, and reported issues about the state of children's oral health in their communities. With ongoing support, the COHA program has a potential to improve access to preventive oral health services in the Medical Home and to increase referrals to a Dental Home. PMID:24228032

  19. Mesomorphic Behavior of Symmetrical and Unsymmetrical Azomethines with Two Imine Groups

    Patrice Rannou

    2009-02-01

    Full Text Available Seven symmetrical azomethines with two imine groups (HC=N were synthesized by condensation of the benzene-1,4-dicarboxaldehydewith five amines (first group: A1-A5 and of the 2,5-thiophenedicarboxaldehyde with two amines (second group: AT1-AT2. Additionally, two unsymmetrical azomethines were obtained by a two step condensation of benzene-1,4-dicarboxaldehydewith pyren-1-amine(1st step (abbreviated hereinafter as AP1 and then AP1 was reacted with4-dodecylaniline or 4-hexadecylaniline (2nd step (third group: AP1A-AP1B. Liquid crystalline properties of the azomethines were studied by differential scanning calorimetry (DSC, polarizing optical microscopy (POM and UV-vis spectroscopy in the function of temperature [UV-vis(T]. The Wide-Angle X-ray Diffraction (WAXD technique was used to probe the structural properties of the azomethines. Mesomorphic behavior was observed for symmetrical and unsymmetrical azomethines, obtained from the benzene-1,4-dicarboxaldehyde and symmetrical ones prepared from 2,5-thiophenedicarboxaldehyde and different amineshaving aliphatic chains. Based on the POM and DSC measurements the following mesophases were detected: nematic, smectic A, smectic C, smectic F (I, smectic G (J.

  20. Isolation and characterization of peptidoglycan recognition protein 1 from antler base of sika deer (Cervus nippon).

    Jiang, Wei; Yin, Yongguang; Zhou, Yajun; He, Guidan; Qi, Yue

    2014-03-01

    Peptidoglycan recognition proteins (PGRPs) are secreted innate immunity pattern recognition molecules. In this study, a new peptidoglycan recognition protein 1 named cnPGRP1 was isolated from an antler base of sika deer Cervus nippon. The antler base antimicrobial proteins (AAP) were subjected to consecutive chromatographic methods connected to Sephadex G-25 gel filtration column (CM) anion-exchange column, and RP-HPLC. The molecular weight of cnPGRP1 was 17.2 kDa under SDS-PAGE, and peptide mass fingerprint analysis by MALDI-TOF-MS as peptidoglycan recognition protein 1 matched to Dasypus novemcinctus. The matched amino acids sequences were RLYEIIQKWPHYRA. Both Gram-positive and Gram-negative bacteria can be killed by cnPGRP1 in the 50-250 μg/mL range through in vitro. Furthermore, cnPGRP1 has been found to bind Gram-positive bacteria, Gram-negative bacteria, and even fungus. PMID:24360898

  1. An artificial neural network application to weekly electrical peak load forecasting

    Artificial neural network (Ann) techniques have been applied to various subjects in the electrical power system area. In this paper, aneural network approach is applied to the weekly load forecast problem. Two neural network (Nn) models for prediction of the seven-day ahead peak load of an electric utility in the region have been developed. The develop ent was based on a nonlinear autoregressive (Ar) time series modeling using simple Backpropation N Ns architecture. These NNmodels were trained using two weeks data window for the most recent daily peak loads. The first model treated the load at weekdays and weekends separately. The second model treated the peak load at weekdays and weekends together with the introduction of indicator variable ms for identifying weekdays from weekends and holidays. The generated forecasts from the two developed models showed that the second model gives more accurate forecasts for the tested utility data. The average absolute percent error (AAPE) for the generated forecasts using this model was 2%. (authors). 18 refs., 5 figs., 2 tabs

  2. HOMOPARENTALIDADE: DA ABORDAGEM CIENTÍFICA AOS NORMATIVOS LEGAIS EM PORTUGAL

    Paula Alexandra Xavier

    2015-04-01

    Full Text Available A homoparentalidade é uma temática que ativa opiniões e tomadas de posição nos vários setores da sociedade que, por sua vez, podem influenciar a postura de cada profissional que trabalha na área das responsabilidades parentais. Neste artigo pretende-se analisar as convergências e dissonâncias entre as disposições da Ordem Jurídica portuguesa e os princípios emergentes da investigação científica adotados por associações como a American Psychological Association (APA e a American Academy of Pediatrics (AAP na área da homoparentalidade. Pretende-se, ainda, fazer uma análise crítica dos estudos reportados pela literatura, ponderando possíveis implicações para a investigação e para as práticas profissionais.

  3. Spectrophotometric Assay of Phenylephrine Hydrochloride Using 4-Aminoantipyrine and Copper (II

    Theia'a N. Al-Sabha

    2010-06-01

    Full Text Available A new spectrophotometric method is proposed for determination of phenylephrine hydrochloride. The method is based on the coupling of 4-aminoantipyrine (4-AAP with phenylephrine hydrochloride (PEH to give a new ligand that reacts with copper (II in the presence of sodium tetraborate buffer solution of pH 9.00 at 50 °C to give an intense red colored chelate having maximum absorption at 480 nm. The optimization of the experimental conditions is described. The method has been used for the determination of 2.0–50.0 μg/ml of PEH. The molar absorptivity is 5.34×103 L.mol.-1cm.-1 and the accuracy of the method is achieved by the value of average recovery (101.28 % and the precision is supported by relative standard deviation (RSD=1.25 % values. The results of the method was compared with those of the standard method. The interference of excipients was studied. The mechanism of the chemical reaction has been proposed. The proposed method was successfully applied for the determination of the PEH in pharmaceutical syrup formulations.

  4. TOPSAR data focusing based on azimuth scaling preprocessing

    Xu, Wei; Huang, Pingping; Deng, Yunkai

    2011-07-01

    Both Doppler spectral aliasing and azimuth output time folding simultaneously exist in TOPSAR (Terrain Observation by Progressive Scans) raw data. Resampling in both Doppler frequency and azimuth time domain can resolve the azimuth aliasing problem but with the seriously increased computational complexity and memory consumption. According to the special characteristics of TOPSAR raw data support in the slow time/frequency domain (TFD), the presented azimuth scaling preprocessing step is introduced to not only resolve the Doppler spectral aliasing problem but also reduce the increased azimuth samples. Furthermore, the correction of sawtoothed azimuth antenna pattern (AAP) becomes easy to be implemented. The following conventional stripmap processor can be adopted to focus the residual TOPSAR raw data but with the result of azimuth aliased TOPSAR image. The mosaic approach, which has been presented to unfold azimuth aliased ScanSAR image, is exploited to resolve the problem of azimuth output folding in TOPSAR mode. Simulation results and pulse response parameters are given to validate the presented imaging approach.

  5. Genome-wide survey and expression analysis of the amino acid transporter superfamily in potato (Solanum tuberosum L.).

    Ma, Haoli; Cao, Xiaoli; Shi, Shandang; Li, Silu; Gao, Junpeng; Ma, Yuling; Zhao, Qin; Chen, Qin

    2016-10-01

    Amino acid transporters (AATs) are integral membrane proteins responsible for the transmembrane transport of amino acids and play important roles in various physiological processes of plants. However, there has not yet been a genome-wide overview of the StAAT gene family to date and only StAAP1 has been previously studied in potato. In this paper, a total of 72 StAATs were identified using a series of bioinformatics searches and classified into 12 subfamilies based on their phylogenetic relationship with known Arabidopsis and rice AATs. Chromosomal localization revealed their distribution on all 12 chromosomes. Nearly one-third of StAAT genes (23 of 72) were derived from gene duplication, among which tandem duplication made the greatest contribution to the expansion of the StAAT family. Motif analysis showed that the same subfamily had similar conserved motifs in both numbers and varieties. Moreover, high-throughput sequencing data was used to analyze the expression patterns of StAAT genes and was verified by quantitative real-time RT-PCR. The expression of StAAT genes exhibited both abundant and tissue-specific expression patterns, which might be connected to their functional roles in long- and short-distance transport. This study provided a comprehensive survey of the StAAT gene family, and could serve as a theoretical foundation for the further functional identification and utilization of family members. PMID:27289266

  6. C2A2 Project - CO2 Capture by Advances Amines process

    This publication presents the operation principles and the obtained results for a research demonstrator developed in Le Havre by EDF and Alstom for CO2 capture by post-combustion. The implemented technology, developed by Alstom and DOX Chemical is named Advanced Amines Processes (AAP). This process comprises the use of solvent and a specific process scheme (the Advanced Flow Scheme or AFS). The smoke treatment chain of the installation is described, and the valorisation of combustion by-products and of smoke processing operations is indicated. The capacities of the installation are given. Systems aimed at increasing the solvent lifetime are described, and some operational parameters are indicated. Various aspects related to the demonstrator design, construction and operation are discussed. Results obtained during tests between October 2013 and March 2014 are given and discussed in terms of quantity of captured CO2, of energy performance, of solvent management and consumption, of emissions, of corrosion, of exploitation organisation, and of instrumentation verification and data quality

  7. Electrosynthesis of polyaniline–mutilwalled carbon nanotube nanocomposite films in the presence of sodium dodecyl sulfate for glucose biosensing

    Polyaniline–mutilwalled carbon nanotube (PANi–MWCNT) nanocomposites were electropolymerized in the presence of sodium dodecyl sulfate (SDS) onto interdigitated platinum-film planar microelectrodes (IDμE). The MWCNTs were first dispersed in SDS solution then mixed with aniline and H2SO4. This mixture was used to electro-synthesize PANi–MWCNT films with potentiostatic method at E = + 0.90 V (versus SCE). The PANi–MWCNT films were characterized by cyclic voltammetry (CV) and scanning electron microscopy (SEM). The results show that the PANi–MWCNT films have a high electroactivity, and a porous and branched structure that can increase the specific surface area for biosensing application. In this work the PANi–MWCNT films were applied for covalent immobilization of glucose oxidase (GOx) via glutaraldehyde agent. The GOx/PANi–MWCNT/IDμE was studied using cyclic voltammetric and chronoamperometric techniques. The effect of several interferences, such as ascorbic acid (AA), uric acid (UA), and acetaminophen (AAP) on the glucosensing at +0.6 V (versus SCE) is not significant. The time required to reach 95% of the maximum steady-state current was less than 5 s. A linear range of the calibration curve for the glucose concentration lies between 1 and 12 mM which is a suitable level in the human body. (paper)

  8. Retrospective review to determine the utility of follow-up skeletal surveys in child abuse evaluations when the initial skeletal survey is normal

    Kachelmeyer Andrea

    2011-09-01

    Full Text Available Abstract Objective The AAP recommends that a follow-up skeletal survey be obtained for all children Methods A retrospective review of radiology records from September 1, 1998 - January 31, 2007 was conducted. Suspected victims of child abuse who were Results Forty-seven children had a negative initial skeletal survey and were included for analysis. The mean age was 6.9 months (SD 5.7; the mean number of days between skeletal surveys was 18.7 (SD 10.1 Four children (8.5% had signs of healing bone trauma on a follow-up skeletal survey. Three of these children (75% had healing rib fractures and one child had a healing proximal humerus fracture. The findings on the follow-up skeletal survey yielded forensically important information in all 4 cases and strengthened the diagnosis of non-accidental trauma. Conclusion 8.5 percent of children with negative initial skeletal surveys had forensically important findings on follow-up skeletal survey that increased the certainty of the diagnosis of non-accidental trauma. A follow-up skeletal survey can be useful even when the initial skeletal survey is negative.

  9. Application of Mn(Ⅱ) as a Mimetic Enzyme of Horseradish Peroxidase

    Ai Xia HAN; Li Hong NIU; Rui CHANG; Fu Shi ZHANG

    2005-01-01

    In this study, Mn( Ⅱ ) as a mimetic enzyme of horseradish peroxidase (HRP) was applied to the determination of hydrogen peroxide (H2O2). The method introduced in this paper is based on Mn(Ⅱ)'s catalytic effect on the oxidation of 4-aminoantipyrine(4-AAP) with modified Trinder's reagent N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3, 5-dimethoxyaniline(DAOS) by H2O2.By coupling this mimetic catalytic reaction with the catalytic reaction of glucose oxidase (GOD),glucose can be detected. Under optimum conditions, the calibration graphs for the determination of H2O2 and glucose are in the range of 1.0×10-3-1.0×10-1 mol/L and 1.0×10-3-14×10-3 mol/L respectively. The detection limit is 5.9×10-4 mol/L for H2O2 and is 9.2×10-4 mol/L for glucose.The feasibility of Mn ( Ⅱ ) as a HRP mimetic enzyme in practical clinical analysis has been proven in the determination of glucose in human serum. So far, Mn ( Ⅱ ) is the simplest and the most inexpensive mimetic enzyme.

  10. The effect of maleinized linseed oil (MLO) on mechanical performance of poly(lactic acid)-thermoplastic starch (PLA-TPS) blends.

    Ferri, J M; Garcia-Garcia, D; Sánchez-Nacher, L; Fenollar, O; Balart, R

    2016-08-20

    In this work, poly(lactic acid), PLA and thermoplastic starch, TPS blends (with a fixed content of 30wt.% TPS) were prepared by melt extrusion process to increase the low ductile properties of PLA. The TPS used contains an aliphatic/aromatic biodegradable polyester (AAPE) that provides good resistance to aging and moisture. This blend provides slightly improved ductile properties with an increase in elongation at break of 21.5% but phase separation is observed due to the lack of strong interactions between the two polymers. Small amounts of maleinized linseed oil (MLO) can positively contribute to improve the ductile properties of these blends by a combined plasticizing-compatibilizing effect. The elongation at break increases over 160% with the only addition of 6phr MLO. One of the evidence of the plasticizing-compatibilizing effect provided by MLO is the change in the glass transition temperature (Tg) with a decrease of about 10°C. Field emission scanning electron microscopy (FESEM) of PLA-TPS blends with varying amounts of maleinized linseed oil also suggests an increase in compatibility. PMID:27178909

  11. Neural Response during the Activation of the Attachment System in Patients with Borderline Personality Disorder: An fMRI Study

    Buchheim, Anna; Erk, Susanne; George, Carol; Kächele, Horst; Martius, Philipp; Pokorny, Dan; Spitzer, Manfred; Walter, Henrik

    2016-01-01

    Individuals with borderline personality disorder (BPD) are characterized by emotional instability, impaired emotion regulation and unresolved attachment patterns associated with abusive childhood experiences. We investigated the neural response during the activation of the attachment system in BPD patients compared to healthy controls using functional magnetic resonance imaging (fMRI). Eleven female patients with BPD without posttraumatic stress disorder (PTSD) and 17 healthy female controls matched for age and education were telling stories in the scanner in response to the Adult Attachment Projective Picture System (AAP), an eight-picture set assessment of adult attachment. The picture set includes theoretically-derived attachment scenes, such as separation, death, threat and potential abuse. The picture presentation order is designed to gradually increase the activation of the attachment system. Each picture stimulus was presented for 2 min. Analyses examine group differences in attachment classifications and neural activation patterns over the course of the task. Unresolved attachment was associated with increasing amygdala activation over the course of the attachment task in patients as well as controls. Unresolved controls, but not patients, showed activation in the right dorsolateral prefrontal cortex (DLPFC) and the rostral cingulate zone (RCZ). We interpret this as a neural signature of BPD patients’ inability to exert top-down control under conditions of attachment distress. These findings point to possible neural mechanisms for underlying affective dysregulation in BPD in the context of attachment trauma and fear. PMID:27531977

  12. Spectrophotometric assay of phenylephrine hydrochloride using 4-aminoantipyrine and copper (II)

    A new spectrophotometric method is proposed for determination of phenylephrine hydrochloride. The method is based on the coupling of 4-aminoantipyrine (4-AAP) with phenylephrine hydrochloride (PEH) to give a new ligand that reacts with copper (II) in the presence of sodium tetraborate buffer solution of pH 9.00 at 50 deg. C to give an intense red colored chelate having maximum absorption at 480 nm. The optimization of the experimental conditions is described. The method has been used for the determination of 2.0 - 50.0 mu g/ml of PEH. The molar absorptivity is 5.34 X 103 L. mol/sup -1/cm /sup- 1/ and the accuracy of the method is achieved by the value of average recovery (101.28 %) and the precision is supported by relative standard deviation (RSD=1.25 %) values. The results of the method was compared with those of the standard method. The interference of excipients was studied. The mechanism of the chemical reaction has been proposed. The proposed method was successfully applied for the determination of the PEH in pharmaceutical syrup formulations. (author)

  13. Chapter Oral Health Advocates: A Nationwide Model for Pediatrician Peer Education and Advocacy about Oral Health

    Charlotte W. Lewis

    2013-01-01

    Full Text Available Objective. (1 To describe an innovative program training US pediatricians to be Chapter Oral Health Advocates (COHAs. (2 To provide insight into COHAs’ experiences disseminating oral health knowledge to fellow pediatricians. Patients and Methods. Interviews with 40 COHAs who responded to an email request, from a total of 64 (62% response. Transcripts were analyzed for common themes about COHA activities, facilitators, and barriers. Results. COHAs reported positive experiences at the AAP oral health training program. A subset of academic COHAs focused on legislative activity and another on resident education about oral health. Residents had an easier time adopting oral health activities while practicing pediatricians cited time constraints. COHAs provided insights into policy, barriers, and facilitators for incorporating oral health into practice. Conclusions. This report identifies factors influencing pediatricians’ adoption of oral health care into practice. COHAs reported successes in training peers on integrating oral health into pediatric practice, identified opportunities and challenges to oral health implementation in primary care, and reported issues about the state of children’s oral health in their communities. With ongoing support, the COHA program has a potential to improve access to preventive oral health services in the Medical Home and to increase referrals to a Dental Home.

  14. Palivizumab use in preterm neonates.

    Kingston, S

    2012-01-31

    Respiratory syncytial virus (RSV) is the leading cause of bronchiolitis in infants. Palivizumab is an immunoprophylactic agent for RSV prevention in preterm infants and those with neonatal chronic lung disease. This study examines its use across neonatal units in Ireland. A questionnaire was administered to one Consultant Neonatologist or Paediatrician in each of the 20 maternity centres in Ireland about their guidelines for Palivizumab administration. There is variation in administration of Palivizumab with little consistency found between protocols reported in terms of age and presence of chronic lung disease. Ten centres have in house protocols, 3 centres use the American Academy of Paediatrics (AAP) guidelines, 2 centres prefer the UK Joint Committee on Vaccination and Immunisation (JCVI) guidelines and 3 centres do not have a set protocol. Four participants felt its use has impacted on hospital admissions and 61% believe its use is cost effective. The budgetary implication for immunoprophylaxis with Palivizumab in Ireland is estimated at 1.5 to 2 million euros annually. Given current pharmacoeconomic constraints there is a need to implement a national protocol on RSV immunoprophylaxis.

  15. Palivizumab use in preterm neonates.

    Kingston, S

    2010-05-01

    Respiratory syncytial virus (RSV) is the leading cause of bronchiolitis in infants. Palivizumab is an immunoprophylactic agent for RSV prevention in preterm infants and those with neonatal chronic lung disease. This study examines its use across neonatal units in Ireland. A questionnaire was administered to one Consultant Neonatologist or Paediatrician in each of the 20 maternity centres in Ireland about their guidelines for Palivizumab administration. There is variation in administration of Palivizumab with little consistency found between protocols reported in terms of age and presence of chronic lung disease. Ten centres have in house protocols, 3 centres use the American Academy of Paediatrics (AAP) guidelines, 2 centres prefer the UK Joint Committee on Vaccination and Immunisation (JCVI) guidelines and 3 centres do not have a set protocol. Four participants felt its use has impacted on hospital admissions and 61% believe its use is cost effective. The budgetary implication for immunoprophylaxis with Palivizumab in Ireland is estimated at 1.5 to 2 million euros annually. Given current pharmacoeconomic constraints there is a need to implement a national protocol on RSV immunoprophylaxis.

  16. Evolution of proteolytic and physico-chemical characteristics of Norwegian dry-cured ham during its processing.

    Petrova, Inna; Tolstorebrov, Ignat; Mora, Leticia; Toldrá, Fidel; Eikevik, Trygve Magne

    2016-11-01

    Proteolytic activity and physico-chemical characteristics were studied for Norwegian dry-cured ham at four different times of processing: raw hams, post-salted hams (3 months of processing), hams selected in the middle of the production (12 months of processing) and hams at the end of the processing (24 months). Cathepsin H activity decreased until negligible values after 3 months of processing, whereas cathepsins B and B+L were inactive at 12 months. AAP was the most active aminopeptidase whereas RAP and MAP were active just during the first 12 months of processing. Proteolysis index reached a value of 4.56±1.03 % with non-significant differences between 12 and 24 months of ripening. Peptide identification by LC-MS/MS was done and two peptides (GVEEPPKGHKGNKK and QAISNNKDQGSY) showing a linear response with the time of processing were found. Unfreezable water content and glass transition temperature were investigated using differential scanning calorimetry (DSC) technique with non-significant differences in the temperature of glass transition for 12 and 24 months of processing. PMID:27371871

  17. Breastfeeding: What are the Barriers? Why Women Struggle to Achieve Their Goals.

    Sriraman, Natasha K; Kellams, Ann

    2016-07-01

    Despite recognized health benefits for both mothers and infants, significant disparities still exist in the rates of breastfeeding in the United States. Major organizations representing the health of women and children (including the Centers for Disease Control and Prevention [CDC], American Academy of Pediatrics [AAP], American Congress of Obstetrics and Gynecology [ACOG], American Academy of Family Physicians [AAFP], United Nations International Children's Emergency Fund (UNICEF), the World Health Organization [WHO], and the United States Public Health Service [PHS]) recommend exclusive breastfeeding, but statistics show that although many women initiate breastfeeding, few meet the recommended goals for duration and exclusivity. This article reviews the evidence related to barriers (prenatal, medical, societal, hospital, and sociocultural) that many mothers face, and explore the known barriers and the impact they have on a woman's ability to breastfeed her infant. Strategies will be discussed to address (and potentially overcome) some of the most common barriers women face along with a list of resources that can be useful in this effort. Gaps in care and areas that need further research will be noted. This article is targeted toward physicians and other healthcare providers who work with women and who can assist with and advocate for the removal of barriers and thereby improve the health of women and children by increasing the rates of breastfeeding initiation, duration, and exclusivity in the United States. PMID:27111125

  18. A proposal to prevent severe neonatal hyperbilirubinemia and kernicterus.

    Bhutani, V K; Johnson, L

    2009-02-01

    To address systems failures and promote a safer management of newborn jaundice, we propose an 'aviation safety standard' for newborn health-care services during the first week after birth. Systems failure in newborn jaundice management has been characterized by lapses in concern, loss of continuity, and delays in care by multiple providers at multiple sites. Components for a six-step national strategy to prevent severe neonatal hyperbilirubinemia and possibly kernicterus are being implemented as delineated in the 2004 AAP guidelines. The clinical guidelines for safer and evidence-based practice have been characterized by both healthcare and societal communities. Professional and community organizations are optimizing outreach resources and facilitating institutionalization of these practices. Nationwide implementation at individual birthing hospitals concurrent with surveillance feedback needs to be initiated. Implementation of a 'six-sigma' approach, as proposed to the current Center for Disease Control and Prevention-initiated partnership (health-care providers, public health and community advocates) may be achieved through collaboration with state and national agencies. PMID:19177062

  19. Neonatal hearing screening program in Ahvaz, Southern Iran

    Marzieh Amiri

    2014-01-01

    Full Text Available Background and Aim: American Academy of Pediatrics (AAP established standards for neonatal hearing screening programs in 1999. The main purpose of this study was to achieve exact statistical data of this program in Ahvaz, Iran, and to compare these results with the standards of American Academy of Pediatrics.Methods: In this cross-sectional descriptive study, the data of neonatal hearing program in Ahvaz west health center from 2008 to 2011 were reviewed. Data were analysed by descriptive statistics.Results: From 25073 neonates, 25 had hearing loss. The false positive and referral ratio to the diagnosis stage were 2.6 and 0.48 percent, respectively. 92 percent of hard of hearing infants were diagnosed before three months of life.Conclusion: In comparison with the standards of American Academy of Pediatrics, all of the items, except two, were included completely or relatively complete. But, more efforts must be done to achieve the whole standards which are available.

  20. Estudo da interação de um begomovírus isolado de tomateiro com a mosca branca Studies of the interaction of a new begomovirus isolate from tomato with the whitefly

    Carmem D. G. Santos

    2003-12-01

    Full Text Available No estudo da interação de um begomovírus isolado de tomateiro (Lycopersicon esculentum em Anápolis-GO, denominado GO-ANPL (taxonomicamente relacionado ao Tomato rugose mosaic virus, ToRMV com o vetor Bemisia tabaci biótipo B, determinaram-se o período de acesso de aquisição do vírus (PAA, o período de acesso de inoculação (PAI, o período de latência (PL, a sua retenção e transmissão à progênie. Nos experimentos empregaram-se plantas de tomateiro 'Santa Clara' e cinco insetos por planta. Constatou-se um PAA mínimo de 15 min com o qual 6% dos tomateiros foram infetados. Este percentual aumentou para 65% quando o PAA foi estendido para 24 h. O PAI mínimo foi de 30 min registrando-se 18% de infecção, o qual foi elevado para 67% com 24 h de PAI. Observou-se o término do PL 16 h após o vetor adquirir o vírus. Na detecção do GO-ANPL no vetor via PCR, testes com mais de 2.500 espécimens constataram o vírus em ninfas desenvolvidas em tomateiro infetado, em adultos com diferentes PAAs, mas não em ovos de fêmeas avirulíferas ovipositados em planta infetada. Observou-se a passagem transestadial em 100% dos adultos testados que transmitiram o vírus em 33% dos casos. Evidenciou-se a transmissão à progênie pela detecção do vírus em ovos, ninfas e adultos provenientes de fêmeas virulíferas, contudo, a transmissão do vírus pelos adultos não foi observada. Os resultados obtidos indicam que a interação vírus-vetor é estabelecida desde a fase inicial do desenvolvimento do inseto e podem ser considerados relevantes para os estudos epidemiológicos dos begomovírus associados ao biótipo B de B. tabaci no país.A begomovirus named GO-ANPL (taxonomically related to the Tomato rugose mosaic virus, ToRMV, obtained from tomato (Lycopesricon esculentum plants in Anápolis, state of Goiás, was used to study virus/vector (Bemisia tabaci biotype B interaction. The acquisition access period (AAP, the inoculation access period

  1. Activation of the attachment system and mentalization in depressive and healthy individuals: An experimental control study

    Fizke Ella

    2013-01-01

    Full Text Available From a developmental and clinical point of view attachment theory and mentalization are closely connected and have become increasingly important to understand the origins of psychopathological development. However, very little is known about how exactly different inner working models of attachment are related to diverse mentalizing abilities and this is particularly true for adult populations - healthy as well as clinical populations. In the present study we investigated this relation with a sample of inpatients diagnosed with depression and a sample of healthy individuals. In an experimental setting the attachment system was activated using the Adult Attachment Projective Picture System (AAP. Mentalization was assessed during activation and in comparison to a control condition using a modified version of the Reading the Mind in the Eyes Test (RMET. We expected that an activation of the attachment system i diminishes the capacity to take another’s perspective in individuals with unresolved state of mind, ii has no impact in individuals with secure attachment representation and iii is dependent of clinical status in individuals with insecure (but organized working models of attachment. Overall, these hypotheses were confirmed. However, the impact of clinical status on mentalization in insecure attachment has to be further explored. We summarize that attachment state of mind has a mediating influence on mentalization basically in such situations where the attachment system is activated.

  2. Newborn Hearing Screening: Experiences of Different Countries

    Aurélio, Fernanda Soares

    2010-09-01

    Full Text Available Introdução: A Triagem Auditiva Neonatal (TAN tem sido implementada em diversos países. Objetivo: O propósito deste estudo foi conhecer e relatar as experiências de diferentes países com a TAN. Método:Realizou-se busca nas bases de dados MEDLINE, BIREME, SCIELO, LILACS, PUBMED, GOOGLE SCHOLAR, Science Direct, periódicos CAPES e nos sites do Grupo de Apoio a Triagem Auditiva Neonatal Universal (GATANU, Joint Committee on Infant Hearing, International Association of Logopedics and Phoniatrics e American Academy of Pediatrics (AAP, além da pesquisa em anais de eventos científicos e livros texto, quando necessário. Os descritores usados foram: triagem auditiva neonatal, triagem auditiva neonatal universal, newborn hearing screening e universal newborn hearing screening. Síntese dos dados: Constatou-se que os programas de TAN vêm sendo realizados em aproximadamente 55 países com eficácia e sucesso. O procedimento predominante é a avaliação de Emissões Otoacústicas Evocadas (EOAEs. Conclusão: São crescentes os esforços pela melhoria da qualidade dos programas, universalização da TAN e elaboração de legislação pertinente.

  3. Assessment of active play, inactivity and perceived barriers in an inner city neighborhood.

    Kottyan, Gregg; Kottyan, Leah; Edwards, Nicholas M; Unaka, Ndidi I

    2014-06-01

    Avondale, a disadvantaged neighborhood in Cincinnati, lags behind on a number of indicators of child well-being. Childhood obesity has become increasingly prevalent, as one-third of Avondale's kindergarteners are obese or overweight. The study objective was to determine perceptions of the quantity of and obstacles to childhood physical activity in the Avondale community. Caregivers of children from two elementary schools were surveyed to assess their child's physical activity and barriers to being active. Three hundred and forty surveys were returned out of 1,047 for a response rate of 32%. On school days, 41% of caregivers reported that their children spent more than 2 h watching television, playing video games, or spending time on the computer. While over half of respondents reported that their children get more than 2 h of physical activity on school days, 14% of children were reported to be physically active less than 1 h per day. Caregivers identified violence, cost of extracurricular activities, and lack of organized activities as barriers to their child's physical activity. The overwhelming majority of caregivers expressed interest in a program to make local playgrounds safer. In conclusion, children in Avondale are not participating in enough physical activity and are exposed to more screen time than is recommended by the AAP. Safety concerns were identified as a critical barrier to address in future advocacy efforts in this community. This project represents an important step toward increasing the physical activity of children in Avondale and engaging the local community. PMID:24306236

  4. Identification and characterization of a resistance gene analog (RGA from the Caricaceae Dumort family Identificação e caracterização de um análogo de gene de resistência (AGR da família de Caricaceae Dumort

    Paulo de Paiva Rosa Amaral

    2006-12-01

    Full Text Available The majority of cloned resistance (R genes characterized so far contain a nucleotide-binding site (NBS and a leucine-rich repeat (LRR domain, where highly conserved motifs are found. Resistance genes analogs (RGAs are genetic markers obtained by a PCR-based strategy using degenerated oligonucleotide primers drawn from these highly conserved "motifs". This strategy has the advantage of the high degree of structural and amino acid sequence conservation that is observed in R genes. The objective of the present study was to search for RGAs in Carica papaya L. and Vasconcellea cauliflora Jacq. A. DC. Out of three combinations of primers tested, only one resulted in amplification. The amplified product was cloned in pCR2.1TOPO and than sequenced using M13 forward and reverse primers. Forty-eight clones were sequenced from each species. The 96 sequences generated for each species were cleaned of vector sequences and clustered using CAP3 assembler. From the GENEBANK, one RGA was identified in C. papaya showing a BlastX e-value of 2x10-61 to the gb|AAP45165.1| putative disease resistant protein RGA3 (Solanum bulbocastanum. To the extent of our knowledge this is the first report of a RGA in the Caricaceae Dumort family. Preliminary structural studies were performed to further characterize this putative NBS-LRR type protein. Efforts to search for other RGAs in papaya should continue, mostly to provide basis for the development of transgenic papaya with resistance to diseases.A maioria dos genes de resistência (R clonados e caracterizados até o momento contém domínios NBS (nucleotide binding site e LRR (leucine-rich repeat. Dentro destes domínios, encontram-se "motifs" altamente conservados. Análogos de genes de resistência (RGAs são marcadores genéticos obtidos por uma estratégia, baseada em PCR, que usa primers degenerados desenhados a partir desses "motifs" altamente conservados dos genes R. Esta estratégia possui a vantagem do elevado grau de

  5. Developments of foreign researches on screen time affecting teenager physical health in recent 10 years%近10年屏幕时间影响青少年体质健康的国外研究进展

    王乐; 张业安; 王磊

    2016-01-01

    基于Web of Science科学引文库(SCI)和社会科学引文库(SSCI),对近10年(2005—2014年)屏幕时间与青少年体质健康相关的英文文献进行综述。同时,参考美国儿科学会(AAP)关于青少年体质健康的相关规定及其演变历程,分析国外关于屏幕时间影响青少年体质健康(肥胖、体育活动参与、相关疾病)的研究进展。文献综述发现:屏幕时间与青少年肥胖的关系,已得到国外研究者的广泛重视和多方面的实证研究;国外相关研究多采用实验的定量研究方法,研究设计较严谨科学,特别是多变量的研究设计值得国内研究者借鉴;在扩展屏幕时间概念外延的基础上,深入研究新媒介传播对青少年体质健康的影响成为该领域未来的研究趋势。%Based on Science Citation Index (SCI) and Social Science Citation Index (SSCI) on the Web of Science, the authors gave an overview of screen time vs. teenager physical health related English literature published in re-cent 10 years (2005-2014), and in the mean time, analyzed developments of foreign researches on screen time af-fecting teenager physical health (obesity, physical activity participation, related diseases) by referring to teenager physical health related stipulations established by American Academy of Pediatrics (AAP) and their course of evo-lution. The literature overview indicates the followings: the relationship between screen time and teenager obesity had been widely valued and empirically studied in multiple aspects by foreign researchers; most of related foreign researches used experimental quantitative research method, their research designs were precise and scientific, espe-cially, their multivariable research designs were worthy of reference for domestic researchers; in-depth study of the affects of new media communication on teenager physical health on the basis of expanding the extension of the concept of screen time is becoming a

  6. Wood anatomy of Mollinedia glabra (Spreng. Perkins (Monimiaceae in two Restinga Vegetation Formations at Rio das Ostras, RJ, Brazil

    Fernanda da S. Novaes

    2010-12-01

    Full Text Available This paper aimed to characterize the anatomical structure of the wood of specimens of Mollinedia glabra (Spreng. Perkins growing in two contiguous formations of restinga vegetation at Praia Virgem, in the municipality of Rio das Ostras, RJ. Both the Open Palmae (OPS and the Sandy Strip Closed Shrub (SSCS formations are found in coastal regions that receive between 1,100 and 1,300 mm of rainfall per year. Sapwood samples were collected in both formations. Typical anatomical features for this species include: solitary vessels, radial multiples or clusters elements, that are circular to angular in outline, 5-15 barred scalarifor perforation plates, ood parenchya scanty, septate fiber-tracheids, and wide multiseriate rays with prismatic crystals. Statistical analyses indicated a significant increase in the frequency of vessel elements and an increase in fiber-tracheid diameters in OPS individuals. These characteristics are considered structural adaptations to increased water needs caused by a greater exposure to sunlight. Continuous pruning may be responsible for the tyloses observed in OPS plants. The greater lengths and higher frequencies of the rays in SSCS trees ay be due to the greater diaeters of their branches. ur results suggest that . glabra develops structural adaptations to the restinga micro-environmental variations during its development.Este trabalho objetiva caracterizar a estrutura anatômica do lenho de Mollinedia glabra, ocorrente em duas formações vegetais contíguas na restinga da Praia Virgem, município de Rio das Ostras, RJ. Essas formações Arbustiva Aberta de Pal-mae (AAP e Arbustiva Fechada do Cordão Arenoso (AFCA estão sobre cordão arenoso e recebem precipitações anuais de 1.100-1.300 mm. Foram obtidas amostras do lenho das duas forações. São características anatôicas gerais da espécie: elementos de vasos solitários, em arranjos radiais ou cachos, de seção de circular a angular; placa de perfuração es

  7. Expression of aggregative adherence to hela cells by Escherichia coli strains isolated from sick horses Expressão de aderência agregativa em células HeLa por amostras de E. coli isoladas de eqüinos doentes

    Ana Maria Alvim Liberatore

    2007-03-01

    Full Text Available The virulence attributes of 56 Escherichia coli strains isolated from sick horses (secretions of uterine cervices; gastrointestinal and lung fragments of necropsy; diarrheic feces, and tracheal washings was examined by determining their adherence pattern to HeLa cells and searching for the presence of virulence genes of the various E. coli pathotypes. Two non-adherent strains presented astA, which encodes the enteroaggregative E. coli heat-stable toxin. Twenty-seven strains (48.2% adhered to HeLa cells, 21 (77.8% of which presented the aggregative adherence pattern (AA that characterize the Enteroaggregative E. coli pathotype (EAEC. Nine of the strains presenting AA were isolated from secretions of uterine cervix, including one carrying virulence genes of the EAEC pathotype (aggR,aap,irp2, and pic. This is the first description of the AA phenotype amongst E. coli strains from sick horses. Such strains should be further evaluated regarding their potential role in the pathogenesis of diverse equine diseases and as reservoirs of human infections.Características de virulência de 56 amostras de Escherichia coli isoladas de eqüinos doentes (secreção de colo uterino, fragmentos de necrópsia do trato gastrointestinal e de pulmões, fezes diarréicas e lavado traqueal foram examinadas para determinar o padrão de aderência em células HeLa e pesquisar a presença de genes de virulência de vários patotipos de E. coli. Duas amostras não aderentes apresentaram astA, gene que codifica a toxina termo-estável de E. coli enteroagregativa. Das vinte e sete amostras (48,2% que aderiram a células HeLa, 21 (77,8% apresentaram o padrão de aderência agregativa (AA que caracteriza o patotipo de E. coli Enteroagregativa (EAEC. Nove destas amostras que apresentaram AA foram isoladas de secreção de colo uterino, incluindo uma que apresentava genes de virulência de patotipos de EAEC (aggR,aap,irp2 e pic. Esta é a primeira descrição do fenótipo AA em

  8. Periodontitis predicts elevated C-reactive protein levels in chronic kidney disease.

    Ioannidou, E; Swede, H; Dongari-Bagtzoglou, A

    2011-12-01

    Based on the existing evidence supporting a state of chronic inflammation in chronic kidney disease (CKD), we hypothesized that periodontal infection may affect the systemic inflammatory status of a nationally representative CKD population as measured by serum C-reactive protein (CRP). We examined this hypothesis using the National Health and Nutrition Examination Survey 1988-1994 (NHANES III) dataset including 2303 individuals. We followed the American Academy of Periodontology (AAP)/Centers for Disease Control and Prevention (CDC) case definition for periodontitis. We used a cutoff point of 30% sites with (PD) ≥ 5 mm and (CAL) ≥ 4 mm to define generalized periodontitis cases. We estimated glomerular filtration rate based on cystatin C levels using the relevant equation. Urinary albumin-to-creatinine ratio was calculated in milligrams per gram with a cutoff point of 30 mg/g. CKD was defined based on eGFR < 60 mL/min/1.73 m(2) and albuminuria ≥ 30 mg/g. Periodontitis was found in 427 (12.3%) individuals. Of individuals with periodontitis, 41.8% had serum CRP higher than 0.3 mg/dL compared with 27.1% of non-periodontitis and 53.1% of edentulous individuals (p = 0.001 for all comparisons). When the extent of periodontitis was used as one of the independent variables, the parsimonious model showed a strong independent association between extent of periodontitis and serum CRP levels (OR = 2.0, CI95% = 1.2-3.6). PMID:21940520

  9. MANAGEMENT OF SIMPLE FEBRILE SEIZURES; A STUDY CONDUCTED IN IMAM HOSPITAL, OROMIEH

    N. Pashapour

    2007-02-01

    Full Text Available ObjectiveWhatever the health field, compliance with the recommended practiceguidelines or parameters for diagnosis by specialists and expert healthprofessionals benefits the patients. This study was conducted to determinethe whether or not these guidelines or parameters are applied to theevaluation of children with first simple febrile convulsion (SFC in a regionalteaching hospital.Materials & MethodsIn a prospective study conducted on children with SFC, admitted in thePediatric ward of Imam hospital, Oromieh, records of investigations orderedbetween Jan 2003 and Dec 2004 for children diagnosed with SFC werecollected. Practice parameters of the American Academy of Pediatrics (AAPwere employed as diagnostic standards. Applied practices were comparedwith AAP recommended practice parameters. Investigations performedincluded lumbar puncture, complete blood count, CRP, ESR, blood glucose,serum calcium, electrolytes, renal function tests, urinalysis, urine culture,and blood culture, chest X-ray, EEG and CT scan.ResultsTwo hundred and fifty-one consecutive cases, aged 6-60 months, wereevaluated. Complete blood count, blood glucose, serum calcium, serumelectrolytes, renal function tests, urinalysis, urine culture, and blood culturewere tested in all cases (100%. Lumbar puncture, chest X-ray, EEG and CTbrain scan had been performed in 10%, 24%, 1.4% and 0.65% of cases,respectively. The mean number of routine investigations was twelve.ConclusionCompared to recommended practice guidelines the results of this studyhighlighted that children with first SFC had more often than necessaryinvestigation. These, not only resulted in significant expense, they provedto be of little diagnostic value. Compliance with a centrally organizednational program would significantly help to improve SFC evaluation.

  10. Quality-by-design: are we there yet?

    Cook, Jack; Cruañes, Maria T; Gupta, Manish; Riley, Steve; Crison, John

    2014-02-01

    In 2012, the Quality-by-Design and Product Performance Focus Group of AAPS conducted a survey to assess the state of adoption and perception of Quality-by-Design (QbD). Responses from 149 anonymous individuals from industry-including consultants-(88%), academia (7%), and regulatory body (4%), were collected. A majority of respondents (54% to 76%) reported high frequency of utilization of several tools and most QbD elements outlined by International Conference on Harmonization Q8, with design of experiments, risk assessment, and the quality target product profile ranked as the top three. Over two thirds of respondents agreed that the benefits of QbD included both the positive impact it can have on the patient (78%), as well as on internal processes such as knowledge management (85%), decision making (79%), and lean manufacture (71%). However, more than 50% from industry were neutral about or disagreed with QbD leading to a better return on investment. This suggests that, despite the recognized scientific, manufacture, and patient-related benefits, there is not yet a clearly articulated business case for QbD available. There was a difference of opinion between industry and regulatory agency respondents as to whether a QbD-based submission resulted in increased efficiency of review. These contrasting views reinforce the idea that QbD implementation can benefit from further dialog between industry and regulatory authorities. A majority of respondents from academia indicated that QbD has influenced their research. In total, the results indicate the broad adoption of QbD but also suggest we are yet in a journey and that the process of gathering all experience and metrics required for connecting and demonstrating QbD benefits to all stakeholders is still in progress. PMID:24218058

  11. Conserved residues Asp16 and Pro24 of TnaC-tRNAPro participate in tryptophan induction of Tna operon expression.

    Cruz-Vera, Luis R; Yanofsky, Charles

    2008-07-01

    In Escherichia coli, interactions between the nascent TnaC-tRNA(Pro) peptidyl-tRNA and the translating ribosome create a tryptophan binding site in the ribosome where bound tryptophan inhibits TnaC-tRNA(Pro) cleavage. This inhibition delays ribosome release, thereby inhibiting Rho factor binding and action, resulting in increased tna operon transcription. Replacing Trp12 of TnaC with any other amino acid residue was previously shown to prevent tryptophan binding and induction of tna operon expression. Genome-wide comparisons of TnaC amino acid sequences identify Asp16 and Pro24, as well as Trp12, as highly conserved TnaC residues. Replacing these residues with other residues was previously shown to influence tryptophan induction of tna operon expression. In this study, in vitro analyses were performed to examine the potential roles of Asp16 and Pro24 in tna operon induction. Replacing Asp16 or Pro24 of TnaC of E. coli with other amino acids established that these residues are essential for free tryptophan binding and inhibition of TnaC-tRNA(Pro) cleavage at the peptidyl transferase center. Asp16 and Pro24 are in fact located in spatial positions corresponding to critical residues of AAP, another ribosome regulatory peptide. Sparsomycin-methylation protection studies further suggested that segments of 23S RNA were arranged differently in ribosomes bearing TnaCs with either the Asp16Ala or the Pro24Ala change. Thus, features of the amino acid sequence of TnaC of the nascent TnaC-tRNA(Pro) peptidyl-tRNA, in addition to the presence of Trp12, are necessary for the nascent peptide to create a tryptophan binding/inhibition site in the translating ribosome. PMID:18424524

  12. Synthesis, Structure and Characterization of New Metal Complexes of Schiff Bases Derived from Isatin N-Benzylisatin and 4-Aminoantipyrine

    *A. J. Abdulghani

    2011-09-01

    Full Text Available The two Schiff bases (z-3-(1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-ylimino indolin-2-one (LIH, and (z-1-benzyl-3-(1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazole-4-yliminoindolin-2-one (LII have been prepared by condensation reaction of isatin and 1-benzyl isatin with 4-aminoantipyrine in dioxane and their structures were characterized by CHN analysis, NMR, IR and mass spectroscopy. A new Hg-metallated biSchiff base ((E-3-(1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-ylimino-2-oxoindolin-1-yl((E-3-(2-methyl-5-oxo-1-phenyl-2,5-dihydro-1H-pyrazol-3-ylimino-2-oxoindolin-1-ylmercury (Hg(LI2 was synthesized by condensation reaction of bis(2,3-dioxoindolin-1-yl mercury(II (Hg(isatin2 with 4-aminoantipyrine(4-AAP. Reaction of LIH with Pt(II and Cu(II complexes in basic medium led to binding of the metal ions at N-1 atom of isatin ring while reaction of LIH and LII with metal salts in neutral medium resulted in the formation of metal complexes where the metal ion is coordinated to carbonyl and azomethine groups of the two ligands. The metal complexes were characterized and their structures were suggested depending on CHNM and thermal analysis, FTIR and electronic spectra, magnetic susceptibility as well as conductivity measurements. The structures of the Ni(II, Pt(II and Hg(II complexes were further supported by NMR spectroscopy.

  13. Study on the drug-resistant and the molecular mechanism of fluoroqninolones resistant Neisseria gonorrhoeae%淋球菌的耐药性分析及其耐氟喹诺酮的分子机制研究

    栾玉泉; 王国富; 吴利先

    2009-01-01

    目的 检测108株临床分离淋球菌(NG)对9种药物的敏感性,并检测喹诺酮类耐药淋球菌对环丙沙星的MIC以及gyrA和parC基因突变情况.方法 用KB法检测淋球菌对9种药物的敏感性,用E-test法检测耐药菌株对环丙沙星的MIC,用PCR和DNA测序及序列比较检测基因突变.结果 淋球菌对大观霉素最敏感,其次为头孢曲松、头孢他啶、阿米卡星、四环素、红霉素和青霉素,对喹诺酮类药物高度耐药.环丙沙星的MIC范围在0.008-32μg/ml之间,10株淋球菌喹诺酮类耐药中有6株存在gyrA的Ser91→Phe突变的同时还存在gyrA的Asp95→Gly和/或parC的Aap86/Asn、Ser87→Arg/Ile,有4株只有gyrA基因Ser91→Phe的突变.结论 在淋球菌治疗中可首选大观霉素,其次为头孢曲松和头孢他啶.gyrA和parC基因突变与淋球菌耐喹诺酮类药物密切相关.

  14. Identification and characterization of CaApe2--a neutral arginine/alanine/leucine-specific metallo-aminopeptidase from Candida albicans.

    Klinke, Thomas; Rump, Andreas; Pönisch, Roman; Schellenberger, Wolfgang; Müller, Eva-Christina; Otto, Albrecht; Klimm, Wolfgang; Kriegel, Thomas M

    2008-09-01

    The proteolytic potential of the pathogenic fungus Candida albicans was evaluated by the identification and functional characterization of a peptidolytic enzyme isolated from the cell wall of the microorganism. Determination of basic structural and kinetic data identified a neutral arginine/alanine/leucine-specific metallo-aminopeptidase of unknown function termed CaApe2, which is encoded by ORF CaO19.5197 (GenBank RefSeq XM_705313). Mass spectrometric tryptic peptide analysis and N-terminal protein sequencing revealed serine-88 to represent the N-terminus of CaApe2. Taking into account the results of DNA and protein sequence analysis including inspection of the genomic region upstream of ORF CaO19.5197, the gene CaAPE2 is likely to consist of two exons linked by a phase-2 intron with exons 1 and 2 encoding a signal peptide and the amino acids 88-954 of ORF CaO19.5197, respectively. The isolated CaApe2 protein shares an equally high similarity with the gene products ScAap1 and ScApe2, suggesting duplication of a phylogenetically ancient precursor gene in Saccharomyces cerevisiae. The observed failure to cleave human type-I and type-IV collagen in vitro challenges a direct role that secreted CaApe2 might play in the degradation of extracellular matrix components during host colonization, but does not exclude per se a contribution of the aminopeptidase to the pathogenicity of C. albicans. PMID:18637841

  15. Cloning and characterization of a xylanase, KRICT PX1 from the strain Paenibacillus sp. HPL-001.

    Hwang, In Taek; Lim, Hee Kyung; Song, Ha Young; Cho, Soo Jin; Chang, Jong-San; Park, No-Joong

    2010-01-01

    The KRICT PX1 gene (GB: FJ380951) consisting of 996bp encoding a protein of 332 amino acids (38.1kDa) from the recently isolated Paenibacillus sp. strain HPL-001 (KCTC11365BP) has been cloned and expressed in Escherichia coli. The xylanase KRICT PX1 showed high activity on birchwood xylan, and was active over a pH range of 5.0 to 11.0, with two optima at pH 5.5 and 9.5 at 50 degrees C with K(m) value of 5.35 and 3.23, respectively. The xylanase activity was not affected by most salts, such as NaCl, LiCl, KCl, NH(4)Cl, CaCl(2), MgCl(2), MnCl(2), and CsCl(2) at 1mM, but affected by CuSO(4), ZnSO(4), and FeCl(3). One mM of EDTA, 2-mercaptoethanol, and PMSF did not affect the xylanase activity. TLC analysis of the catalyzed products after reaction with birchwood xylan revealed that xylobiose was the major product with smaller amounts of xylotriose and xylose. A similarity analysis of the amino acids in KRICT PX1 resulted 72% identity with xylanase from Geobacillus stearothermophilus (GB: ZP_03040360), 70% identity with intracellular xylanase from an uncultured bacterium (GB: AAP51133), 68% identity with endo-1-4-xylanse from Paenibacillus sp. (GB: ZP_02847150). In addition, the amino acid alignment of KRICT PX1 with glycosyl hydralase (GH) family 10 xylanases revealed a high degree of homology in highly conserved regions including the catalytic sites, and this was confirmed through PROSITE scan. These results imply that KRICT PX1 is a new xylanase gene, and this alkaline xylanase belongs to GH family 10. PMID:20493247

  16. Combining Areal and Point Data in Geostatistical Interpolation: Applications to Soil Science and Medical Geography.

    Goovaerts, Pierre

    2010-07-01

    A common issue in spatial interpolation is the combination of data measured over different spatial supports. For example, information available for mapping disease risk typically includes point data (e.g. patients' and controls' residence) and aggregated data (e.g. socio-demographic and economic attributes recorded at the census track level). Similarly, soil measurements at discrete locations in the field are often supplemented with choropleth maps (e.g. soil or geological maps) that model the spatial distribution of soil attributes as the juxtaposition of polygons (areas) with constant values. This paper presents a general formulation of kriging that allows the combination of both point and areal data through the use of area-to-area, area-to-point, and point-to-point covariances in the kriging system. The procedure is illustrated using two data sets: (1) geological map and heavy metal concentrations recorded in the topsoil of the Swiss Jura, and (2) incidence rates of late-stage breast cancer diagnosis per census tract and location of patient residences for three counties in Michigan. In the second case, the kriging system includes an error variance term derived according to the binomial distribution to account for varying degree of reliability of incidence rates depending on the total number of cases recorded in those tracts. Except under the binomial kriging framework, area-and-point (AAP) kriging ensures the coherence of the prediction so that the average of interpolated values within each mapping unit is equal to the original areal datum. The relationships between binomial kriging, Poisson kriging, and indicator kriging are discussed under different scenarios for the population size and spatial support. Sensitivity analysis demonstrates the smaller smoothing and greater prediction accuracy of the new procedure over ordinary and traditional residual kriging based on the assumption that the local mean is constant within each mapping unit. PMID:21132098

  17. IR, UV-Vis, magnetic and thermal characterization of chelates of some catecholamines and 4-aminoantipyrine with Fe(III) and Cu(II)

    Mohamed, Gehad G.; Zayed, M. A.; El-Dien, F. A. Nour; El-Nahas, Reham G.

    2004-07-01

    The dopamine derivatives participate in the regulation of wide variety of physiological functions in the human body and in medication life. Increase and/or decrease in the concentration of dopamine in human body reflect an indication for diseases such as Schizophrenia and/or Parkinson diseases. α-Methyldopa (α-MD) in tablets is used in medication of hypertension. The Fe(III) and Cu(II) chelates with coupled products of adrenaline hydrogen tartarate (AHT), levodopa (LD), α-MD and carbidopa (CD) with 4-aminoantipyrine (4-AAP) are prepared and characterized. Different physico-chemical methods like IR, magnetic and UV-Vis spectra are used to investigate the structure of these chelates. Fe(III) form 1:2 (M:catecholamines) chelates while Cu(II) form 1:1 chelates. Catecholamines behave as a bidentate mono- or dibasic ligands in binding to the metal ions. IR spectra show that the catecholamines are coordinated to the metal ions in a bidentate manner with O,O donor sites of the phenolic - OH. Magnetic moment measurements reveal the presence of Fe(III) chelates in octahedral geometry while the Cu(II) chelates are square planar. The thermal decomposition of Fe(III) and Cu(II) complexes is studied using thermogravimetric (TGA) and differential thermal analysis (DTA) techniques. The water molecules are removed in the first step followed immediately by decomposition of the ligand molecules. The activation thermodynamic parameters, such as, energy of activation, enthalpy, entropy and free energy change of the complexes are evaluated and the relative thermal stability of the complexes are discussed.

  18. Cortical spreading depression: An enigma

    Miura, R. M.; Huang, H.; Wylie, J. J.

    2007-08-01

    The brain is a complex organ with active components composed largely of neurons, glial cells, and blood vessels. There exists an enormous experimental and theoretical literature on the mechanisms involved in the functioning of the brain, but we still do not have a good understanding of how it works on a gross mechanistic level. In general, the brain maintains a homeostatic state with relatively small ion concentration changes, the major ions being sodium, potassium, and chloride. Calcium ions are present in smaller quantities but still play an important role in many phenomena. Cortical spreading depression (CSD for short) was discovered over 60 years ago by A.A.P. Leão, a Brazilian physiologist doing his doctoral research on epilepsy at Harvard University, “Spreading depression of activity in the cerebral cortex," J. Neurophysiol., 7 (1944), pp. 359-390. Cortical spreading depression is characterized by massive changes in ionic concentrations and slow nonlinear chemical waves, with speeds on the order of mm/min, in the cortex of different brain structures in various experimental animals. In humans, CSD is associated with migraine with aura, where a light scintillation in the visual field propagates, then disappears, and is followed by a sustained headache. To date, CSD remains an enigma, and further detailed experimental and theoretical investigations are needed to develop a comprehensive picture of the diverse mechanisms involved in producing CSD. A number of mechanisms have been hypothesized to be important for CSD wave propagation. In this paper, we briefly describe several characteristics of CSD wave propagation, and examine some of the mechanisms that are believed to be important, including ion diffusion, membrane ionic currents, osmotic effects, spatial buffering, neurotransmitter substances, gap junctions, metabolic pumps, and synaptic connections. Continuum models of CSD, consisting of coupled nonlinear diffusion equations for the ion concentrations, and

  19. Sensitive and selective determining ascorbic acid and activity of alkaline phosphatase based on electrochemiluminescence of dual-stabilizers-capped CdSe quantum dots in carbon nanotube-nafion composite.

    Ma, Xiaolong; Zhang, Xin; Guo, Xinli; Kang, Qi; Shen, Dazhong; Zou, Guizheng

    2016-07-01

    Sensitive and selective determining bio-related molecule and enzyme play an important role in designing novel procedure for biological sensing and clinical diagnosis. Herein, we found that dual-stabilizers-capped CdSe quantum dots (QDs) in composite film of multi-walled carbon nanotubes (CNTs) and Nafion, displaying eye-visible monochromatic electrochemiluminescence (ECL) with fwhm of 37nm, which offers promising ECL signal for detecting ascorbic acid (AA) as well as the activity of alkaline phosphatase (ALP) in biological samples. It was also shown that the dual-stabilizers-capped CdSe QDs can preserve their highly passivated surface states with prolonged lifetime of excited states in Nafion mixtures, and facilitate electron-transfer ability of Nafion film along with CNTs. Compared with the QDs/GCE, the ECL intensity is enhanced 1.8 times and triggering potential shifted to lower energy by 0.12V on the CdSe-CNTs-Nafion/GCE. The ECL quenching degree increases with increasing concentration of AA in the range of 0.01-30nM with a limit of detection (LOD) of 5pM. The activity of ALP was determined indirectly according to the concentration of AA, generated in the hydrolysis reaction of l-ascorbic acid 2-phosphate sesquimagnesium (AA-P) in the presence of ALP as a catalyst, with an LOD of 1μU/L. The proposed strategy is favorable for developing simple ECL sensor or device with high sensitivity, spectral resolution and less electrochemical interference. PMID:27154663

  20. Immobilization of phosphorus from water and sediment using zirconium-modified zeolites.

    Yang, Mengjuan; Lin, Jianwei; Zhan, Yanhui; Zhu, Zhiliang; Zhang, Honghua

    2015-03-01

    Adding sorbents to sediments has been suggested as an effective technology for contaminated sediment remediation. In this study, a zirconium-modified zeolite (ZrMZ) was prepared, characterized, and used as a sediment amendment to control phosphorus (P) release from eutrophic lake sediments. The efficiency of ZrMZ in immobilizing P from water and sediments was investigated through a series of experiments. The phosphate adsorption capacity for ZrMZ decreased with increasing water pH. The adsorption of phosphate on ZrMZ followed a pseudo-second-order kinetic model. The equilibrium adsorption data of phosphate on ZrMZ could be well described by the Langmuir isotherm model with a maximum monolayer adsorption capacity of 10.2 mg P/g at pH 7 and 25 °C. Sequential extraction of P from the phosphate-adsorbed ZrMZ suggested that most of P bound by ZrMZ existed as the NaOH extractable P (NaOH-P) and residual P (Res-P) and was unlikely to be released under natural pH and reducing conditions. The addition of ZrMZ into sediments reduced the inorganic P activity in the sediments by transforming bicarbonate-dithionite extractable P (BD-P) to NaOH-P and Res-P. The contents of bioavailable P such as water-soluble P (WS-P), NaHCO3 extractable P (Olsen-P), and algal available P (AAP) in sediments reduced after the sediments were mixed with ZrMZ, making P in the sediments more stable. The addition of ZrMZ into sediments significantly reduced the releasing flux of P from the sediments to the water column under different conditions. Results of this study indicate that the ZrMZ is a promising sediment amendment for controlling the internal P loading of lake sediments. PMID:25253056

  1. Quantitative determination of sulfisoxazole and its three N-acetylated metabolites using HPLC-MS/MS, and the saturable pharmacokinetics of sulfisoxazole in mice.

    Oh, Kyungsoo; Baek, Moon-Chang; Kang, Wonku

    2016-09-10

    Sulfisoxazole (SFX) is still used in combination with trimethoprim in cattle despite adverse drug reactions (e.g., urolithiasis). Recently, SFX is known to be a promising repositioned drug candidate for pulmonary hypertension and cancer. We developed a simultaneous determination method of SFX and its N-acetylated metabolites (N(1)-acetyl SFX, N1AS; N(4)-acetyl SFX, N4AS; diacetyl SFX, DAS) using HPLC-MS/MS for the first time, and examined the pharmacokinetics of SFX in mice. N1AS and DAS were converted rapidly to SFX and N4AS, respectively, in mouse plasma. The time courses of plasma SFX and N4AS concentrations were well-characterised following the oral administration of SFX to mice. The absorption, metabolism, and/or excretion of SFX given at >700mg/kg may be saturable, and in contrast to humans and rats, the extent of systemic exposure of mice to N4AS was much greater than that of SFX. Interestingly, the acetyl groups at both N1- and N4-positions were degraded during the ionisation required to generate precursor ions. In additional experiments the carboxyl group of N-acetyl-5-aminosalicylic acid (NA5AS) was lost instead of the acetyl group during the ionisation, and acetaminophen (AAP) appeared. As the acetyl and carboxyl groups of some substances can be degraded during ionisation in the mass spectrometer, caution is appropriate when it is sought to simultaneously quantify similar structures containing these moieties; chromatographic separation is essential. PMID:27454084

  2. Integration of small RNAs, degradome and transcriptome sequencing in hyperaccumulator Sedum alfredii uncovers a complex regulatory network and provides insights into cadmium phytoremediation.

    Han, Xiaojiao; Yin, Hengfu; Song, Xixi; Zhang, Yunxing; Liu, Mingying; Sang, Jiang; Jiang, Jing; Li, Jihong; Zhuo, Renying

    2016-06-01

    The hyperaccumulating ecotype of Sedum alfredii Hance is a cadmium (Cd)/zinc/lead co-hyperaccumulating species of Crassulaceae. It is a promising phytoremediation candidate accumulating substantial heavy metal ions without obvious signs of poisoning. However, few studies have focused on the regulatory roles of miRNAs and their targets in the hyperaccumulating ecotype of S. alfredii. Here, we combined analyses of the transcriptomics, sRNAs and the degradome to generate a comprehensive resource focused on identifying key regulatory miRNA-target circuits under Cd stress. A total of 87 721 unigenes and 356 miRNAs were identified by deep sequencing, and 79 miRNAs were differentially expressed under Cd stress. Furthermore, 754 target genes of 194 miRNAs were validated by degradome sequencing. A gene ontology (GO) enrichment analysis of differential miRNA targets revealed that auxin, redox-related secondary metabolism and metal transport pathways responded to Cd stress. An integrated analysis uncovered 39 pairs of miRNA targets that displayed negatively correlated expression profiles. Ten miRNA-target pairs also exhibited negative correlations according to a real-time quantitative PCR analysis. Moreover, a coexpression regulatory network was constructed based on profiles of differentially expressed genes. Two hub genes, ARF4 (auxin response factor 4) and AAP3 (amino acid permease 3), which might play central roles in the regulation of Cd-responsive genes, were uncovered. These results suggest that comprehensive analyses of the transcriptomics, sRNAs and the degradome provided a useful platform for investigating Cd hyperaccumulation in S. alfredii, and may provide new insights into the genetic engineering of phytoremediation. PMID:26801211

  3. An investigation of the diversity of strains of enteroaggregative Escherichia coli isolated from cases associated with a large multi-pathogen foodborne outbreak in the UK.

    Timothy J Dallman

    Full Text Available Following a large outbreak of foodborne gastrointestinal (GI disease, a multiplex PCR approach was used retrospectively to investigate faecal specimens from 88 of the 413 reported cases. Gene targets from a range of bacterial GI pathogens were detected, including Salmonella species, Shigella species and Shiga toxin-producing Escherichia coli, with the majority (75% of faecal specimens being PCR positive for aggR associated with the Enteroaggregative E. coli (EAEC group. The 20 isolates of EAEC recovered from the outbreak specimens exhibited a range of serotypes, the most frequent being O104:H4 and O131:H27. None of the EAEC isolates had the Shiga toxin (stx genes. Multilocus sequence typing and single nucleotide polymorphism analysis of the core genome confirmed the diverse phylogeny of the strains. The analysis also revealed a close phylogenetic relationship between the EAEC O104:H4 strains in this outbreak and the strain of E. coli O104:H4 associated with a large outbreak of haemolytic ureamic syndrome in Germany in 2011. Further analysis of the EAEC plasmids, encoding the key enteroaggregative virulence genes, showed diversity with respect to FIB/FII type, gene content and genomic architecture. Known EAEC virulence genes, such as aggR, aat and aap, were present in all but one of the strains. A variety of fimbrial genes were observed, including genes encoding all five known fimbrial types, AAF/1 to AAF/V. The AAI operon was present in its entirety in 15 of the EAEC strains, absent in three and present, but incomplete, in two isolates. EAEC is known to be a diverse pathotype and this study demonstrates that a high level of diversity in strains recovered from cases associated with a single outbreak. Although the EAEC in this study did not carry the stx genes, this outbreak provides further evidence of the pathogenic potential of the EAEC O104:H4 serotype.

  4. Oxidant/antioxidant status during foal heat in Arab mares and their relation to ovarian hormones

    Amal M AboEl-Maaty; Fawzia YH Shata; Manal B-E Mahmoud; Faten I Gabr

    2012-01-01

    Objective:To study the role of some oxidative stress biomarkers in conception during foal heat. Methods:Ultrasound scanning was used to confirm the presence of mature ovulating follicle (>30mm) in mares exhibited foal heat on days7 to9 postpartum.Daily blood samples were collected from parturition till foal heat to measure equine lysozymes, nitric oxide(NO), catalase (CAT), total proteins and globulins.Lipid peroxide(MDA), reduced glutathione(GSH), ascorbic acid(AA) and zinc were measured during foal heat expression form day7 to10 post foaling. Results:Lysozymes were high on days1 and9 postpartum but low level was recorded on day 6.NO, total proteins and globulin peaked on days5 and6 but low level was recorded on day9. HighMDA(P<0.03), zinc(P<0.05) levels were observed on days8 and9.HighGSH was observed on day9.AA(P<0.03) andCAT levels were low on days8 and9.Non conceived mares had high MDA, low zinc,AA,CAT andGSH.Conclusions:Mares during the foal heat are subjected to oxidative stress on days8 and9 as expressed by lowAA, zinc, andGSH and highMDA and mares with sufficient antioxidant capacity can overcome this stress and conceive.Some mares need to be supplied during foal heat with antioxidants to improve their immunity and overcome oxidant/antioxidant imbalance to conceive.This study recommends the use of ascorbic acid and zinc as supplements during foal heat.

  5. Enzymatic Determination of Glucose by Optical-Fiber Sensor Sequential Injection Renewable Surface Spectrophotometry

    2006-01-01

    On the basis of oxidative decoloration of bromopyrogallol red(BPR) with H2O2, catalyzed by horseradish peroxidase(HRP), and the sequential injection renewable surface technique(SI-RST), a highly sensitive optical-fiber sensor spectrophotometric method for the enzymatic determination of hydrogen peroxide was proposed. By coupling with a glucose oxidase(GOD) -catalyzed reaction, the method was used to determine glucose in human serum. The considerations in system and flow cell design, and factors that influence the determination performance are discussed. With 100 μL of sample loaded and 0. 6 mg of bead trapped, the linear response range from 5.0 × 10-8 to 5. 2 × 10-6mol/L BPR with a detection limit(3σ) of 2. 5 × 10 -8 mol/L BPR, and a precision of 1.1% RSD(n = 11) and a throughput of a 80 samples per hour can be achieved. Under the conditions of a 8. 7 × 10-6 mol/L BPR substrate,0.04 unit/mL HRP, 600 s reaction time and a reaction temperature of 37 ℃, the linear response range for H2O2 was from 5.0 × 10-8 to 7.0 × 10-6 mol/L with a detection limit(3σ) of 1.0 × 10-8 mol/L and a precision of 3.7% RSD(n=11). The linear response range by coupling with a GOD-catalyzed reaction was from 1.0 ×10-7 to 1.0 × 10-5mol/L. The method was directly applied to determine glucose in human serum. Glucose contents obtained by the proposed procedure were compared with those obtained by using the phenol-4-AAP method, the error was found to be less than 3%.

  6. [Acute pyelonephritis in the neonatal period] [Article in Italian] • Pielonefrite acuta in epoca neonatale

    Giovanni Ottonello

    2014-01-01

    Full Text Available Urinary tract infections (UTIs represent one of the most common pathological conditions in children. It is estimated that even in the countries with the most advanced health care programmes, such as the United States and Europe, UTIs represent the most common serious infections in pediatrics. In the earliest stages of life, UTIs represent the most common febrile illness, although the non specific clinical presentation often leads to diagnostic underestimation. Neonatal UTIs surely represent a special case among pediatric UTIs and need specific considerations and separate treatment with respect to later age: it is no coincidence that the main national and international guidelines for the diagnosis and treatment of UTIs, such as those issued by the American Academy of Pediatrics (AAP and by the Working Group of the Italian Society of Pediatric Nephrology, exclude from their indications the newborn and the infant under two months of age. Low gestational age, low birth weight, association with malformative uropathies are considered specific risk factors. Breastfeeding has a protective effect. E. coli is the major responsible of UTI in term newborns and infants, followed by K. pneumoniae, Proteus spp., E. cloacae. Finally, Candida spp. is very important in hospitalized children. Treatment includes the combination of ampicillin plus an aminoglycoside (frequently gentamicin. The article shows the main aspects of the urinary tract infections in the neonatal period and a review of the most recent literature on the subject.Articoli Selezionati del “3° Convegno Pediatrico del Medio Campidano” · Guspini · 25 Maggio 2013 Guest Editor: Roberto Antonucci

  7. Coherent measures of the impact of co-authors in peer review journals and in proceedings publications

    Ausloos, Marcel

    2015-11-01

    This paper focuses on the coauthor effect in different types of publications, usually not equally respected in measuring research impact. A priori unexpected relationships are found between the total coauthor core value, ma, of a leading investigator (LI), and the related values for their publications in either peer review journals (j) or in proceedings (p). A surprisingly linear relationship is found: ma(j) + 0.4 ma(p) = ma(jp) . Furthermore, another relationship is found concerning the measure of the total number of citations, Aa, i.e. the surface of the citation size-rank histogram up to ma. Another linear relationship exists : Aa(j) + 1.36 Aa(p) = Aa(jp) . These empirical findings coefficients (0.4 and 1.36) are supported by considerations based on an empirical power law found between the number of joint publications of an author and the rank of a coauthor. Moreover, a simple power law relationship is found between ma and the number (rM) of coauthors of an LI: ma ≃ rMμ ; the power law exponent μ depends on the type (j or p) of publications. These simple relations, at this time limited to publications in physics, imply that coauthors are a "more positive measure" of a principal investigator role, in both types of scientific outputs, than the Hirsch index could indicate. Therefore, to scorn upon co-authors in publications, in particular in proceedings, is incorrect. On the contrary, the findings suggest an immediate test of coherence of scientific authorship in scientific policy processes.

  8. The Development and Predictive Research of the "Anti-social Abnormal Personality Scale"%反社会变态人格倾向测验的编制与预测性分析

    王伟; 张石磊; 关慕桢; 李红政; 刘旭峰

    2011-01-01

    the discussion of 22 experts and the validity and the reliability were tested. As items or questions are the basis of a questionnaire or a scale. We first analyzed some key literature and listed 6 high specific behavior sources d anti-social personality: (1) From parents' bad behaviors to mischievous tricks; (2)From being spoiled, to lording it over and finally to attacking; (3)From a one- poor to dual-poor student and playing truant in groups; (4) From pursuit of adventure to running away from home and violating the rights of others;(5)From being discriminated against or abused to taking revenge and attacking, and (6)From being indulged in the Internet and imitating violence to putting into practice. Based on these 6 specific behavior sources, we got the final version d the anti- social abnormal personality scale (AAPS), which was composed of 168 items. In the second step, the prediction of AAPS was evaluated by four group subjects. The results showed that AAPS had a three-dimension structure with Deviate (Dev), Impulsive (Imp) and Antisocial (Ant). The internal consistency of 3 clinical subscales was assessed with Cronbach's alpha. The a's were . 84 for Dev, . 88 for Imp and. 80 for Ant respectively. The test-retest coefficients all exceeded. 74 with an interval of 30 days. The total accuracy rate of discrimination was 85.7 % and the classification results indicated that the discriminant function was more stable. Based on the practical predictive validity tests, the accuracy rate of discrimination for candidates and the average predictive coincidence rate of soldiers being followed for one year reached up to 72.55 % and 98.12 % respectively. The conclusion can be drawn based on the above data that AAPS accords with the behavioral characteristics of the target group, has good validity and reliability and the be used as an effective tool to evaluate whether youth candidates have the tendency of anti-social personality

  9. A process for developing community consensus regarding the diagnosis and management of attention-deficit/hyperactivity disorder.

    Foy, Jane Meschan; Earls, Marian F

    2005-01-01

    There remain large discrepancies between pediatricians' practice patterns and the American Academy of Pediatrics (AAP) guidelines for the assessment and treatment of children with attention-deficit/hyperactivity disorder (ADHD). Several studies raise additional concerns about access to ADHD treatment for girls, blacks, and poorer individuals. Barriers may occur at multiple levels, including identification and referral by school personnel, parents' help-seeking behavior, diagnosis by the medical provider, treatment decisions, and acceptance of treatment. Such findings confirm the importance of establishing appropriate mechanisms to ensure that children of both genders and all socioeconomic, racial, and ethnic groups receive appropriate assessment and treatment. Publication of the AAP ADHD toolkit provides resources to assist with implementing the ADHD guidelines in clinical practice. These resources address a number of the barriers to office implementation, including unfamiliarity with Diagnostic and Statistical Manual of Mental Disorders criteria, difficulty identifying comorbidities, and inadequate knowledge of effective coding practices. Also crucial to the success of improved processes within clinical practice is community collaboration in care, particularly collaboration with the educational system. Such collaboration addresses other barriers to good care, such as pressures from parents and schools to prescribe stimulants, cultural biases that may prevent schools from assessing children for ADHD or may prevent families from seeking health care, and inconsistencies in recognition and referral among schools in the same system. Collaboration may also create efficiencies in collection of data and school-physician communications, thereby decreasing physicians' non-face-to-face (and thus nonreimbursable) elements of care. This article describes a process used in Guilford County, North Carolina, to develop a consensus among health care providers, educators, and child

  10. Angiotensin II-induced Akt activation through the epidermal growth factor receptor in vascular smooth muscle cells is mediated by phospholipid metabolites derived by activation of phospholipase D.

    Li, Fang; Malik, Kafait U

    2005-03-01

    Angiotensin II (Ang II) activates cytosolic Ca(2+)-dependent phospholipase A(2) (cPLA(2)), phospholipase D (PLD), p38 mitogen-activated protein kinase (MAPK), epidermal growth factor receptor (EGFR) and Akt in vascular smooth muscle cells (VSMC). This study was conducted to investigate the relationship between Akt activation by Ang II and other signaling molecules in rat VSMC. Ang II-induced Akt phosphorylation was significantly reduced by the PLD inhibitor 1-butanol, but not by its inactive analog 2-butanol, and by brefeldin A, an inhibitor of the PLD cofactor ADP-ribosylation factor, and in cells infected with retrovirus containing PLD(2) siRNA or transfected with PLD(2) antisense but not control LacZ or sense oligonucleotide. Diacylglycerol kinase inhibitor II diminished Ang II-induced and diC8-phosphatidic acid (PA)-increased Akt phosphorylation, suggesting that PLD-dependent Akt activation is mediated by PA. Ang II-induced EGFR phosphorylation was inhibited by 1-butanol and PLD(2) siRNA and also by cPLA(2) siRNA. In addition, the inhibitor of arachidonic acid (AA) metabolism 5,8,11,14-eicosatetraynoic acid (ETYA) reduced both Ang II- and AA-induced EGFR transactivation. Furthermore, ETYA, cPLA(2) antisense, and cPLA(2) siRNA attenuated Ang II-elicited PLD activation. p38 MAPK inhibitor SB202190 [4-(4-flurophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)1H-imidazole] reduced PLD activity and EGFR and Akt phosphorylation elicited by Ang II. Pyrrolidine-1, a cPLA(2) inhibitor, and cPLA(2) siRNA decreased p38 MAPK activity. These data indicate that Ang II-stimulated Akt activity is mediated by cPLA(2)-dependent, p38 MAPK regulated PLD(2) activation and EGFR transactivation. We propose the following scheme of the sequence of events leading to activation of Akt in VSMC by Ang II: Ang II-->cPLA(2)-->AA-->p38 MAPK-->PLD(2)-->PA-->EGFR-->Akt. PMID:15525798

  11. Defensive nature of Sargassum polycystum (Brown alga)against acetaminophen-induced toxic hepatitis in rats: Role of drug metabolizing microsomal enzyme system, tumor necrosis factor-α and fate of liver cell structural integrity

    H Balaji raghavendran; A Sathivel; T Devaki

    2006-01-01

    AIM: To assess the defensive nature of Sargassum polycystum (S. Polycystum) (Brown alga) against acetaminophen (AAP)-induced changes in drug metabolizing microsomal enzyme system, tumor necrosis factor (TNF-α)and fine structural features of the liver during toxic hepatitis in rats.METHODS: Male albino Wistar strain rats used for the study were randomly categorized into 4 groups. Group Ⅰ consisted of normal control rats fed with standard diet.Group Ⅱ rats were administered with acetaminophen (800 mg/kg body weight, intraperitoneally). Group Ⅲ rats were pre-treated with S. Polycystum extract alone.Group Ⅳ rats were orally pre-treated with S. Polycystum extract (200 mg/kg body weight for 21 d) prior to acetaminophen induction (800 mg/kg body weight,intraperitoneally). Serum separated and liver was excised and microsomal fraction was isolated for assaying cytochrome P450, NADPH Cyt P450 reductase and b5.Serum TNF-α was detected using ELISA. Fine structural features of liver were examined by transmission electron microscopy.RESULTS: Rats intoxicated with acetaminophen showed considerable impairment in the activities of drug metabolizing microsomal enzymes, such as cytochrome P450, NADPH Cyt P450 reductase and b5 when compared with the control rats. The rats intoxicated with acetaminophen also significantly triggered serum TNF-α when compared with the control rats. These severe alterations in the drug metabolizing enzymes were appreciably prevented in the rats pretreated with S. Polycystum. The rats pretreated with S. Polycystum showed considerable inhibition in the elevation of TNF-α compared to the rats intoxicated with acetaminophen. The electron microscopic observation showed considerable loss of structural integrity of the endoplasmic reticulum, lipid infiltration and ballooning of mitochondria in the acetaminophen-intoxicated rats,whereas the rats treated with S. Polycystum showed considerable protection against acetaminophen-induced alterations in

  12. Improved method for linear B-cell epitope prediction using antigen's primary sequence.

    Harinder Singh

    Full Text Available One of the major challenges in designing a peptide-based vaccine is the identification of antigenic regions in an antigen that can stimulate B-cell's response, also called B-cell epitopes. In the past, several methods have been developed for the prediction of conformational and linear (or continuous B-cell epitopes. However, the existing methods for predicting linear B-cell epitopes are far from perfection. In this study, an attempt has been made to develop an improved method for predicting linear B-cell epitopes. We have retrieved experimentally validated B-cell epitopes as well as non B-cell epitopes from Immune Epitope Database and derived two types of datasets called Lbtope_Variable and Lbtope_Fixed length datasets. The Lbtope_Variable dataset contains 14876 B-cell epitope and 23321 non-epitopes of variable length where as Lbtope_Fixed length dataset contains 12063 B-cell epitopes and 20589 non-epitopes of fixed length. We also evaluated the performance of models on above datasets after removing highly identical peptides from the datasets. In addition, we have derived third dataset Lbtope_Confirm having 1042 epitopes and 1795 non-epitopes where each epitope or non-epitope has been experimentally validated in at least two studies. A number of models have been developed to discriminate epitopes and non-epitopes using different machine-learning techniques like Support Vector Machine, and K-Nearest Neighbor. We achieved accuracy from ∼54% to 86% using diverse s features like binary profile, dipeptide composition, AAP (amino acid pair profile. In this study, for the first time experimentally validated non B-cell epitopes have been used for developing method for predicting linear B-cell epitopes. In previous studies, random peptides have been used as non B-cell epitopes. In order to provide service to scientific community, a web server LBtope has been developed for predicting and designing B-cell epitopes (http://crdd.osdd.net/raghava/lbtope/.

  13. Geoethics: what can we learn from existing bio-, ecological, and engineering ethics codes?

    Kieffer, Susan W.; Palka, John

    2014-05-01

    Many scientific disciplines are concerned about ethics, and codes of ethics for these professions exist, generally through the professional scientific societies such as the American Geophysical Union (AGU), American Geological Institute (AGI), American Association of Petroleum Engineers (AAPE), National Society of Professional Engineers (NSPE), Ecological Society of America (ESA), and many others worldwide. These vary considerably in depth and specificity. In this poster, we review existing codes with the goal of extracting fundamentals that should/can be broadly applied to all geo-disciplines. Most of these codes elucidate a set of principles that cover practical issues such as avoiding conflict of interest, avoiding plagiarism, not permitting illegitimate use of intellectual products, enhancing the prestige of the profession, acknowledging an obligation to perform services only in areas of competence, issuing public statements only in an objective manner, holding paramount the welfare of the public, and in general conducting oneself honorably, responsibly, and lawfully. It is striking that, given that the work of these societies and their members is relevant to the future of the earth, few discuss in any detail ethical obligations regarding our relation to the planet itself. The AGU code, for example, only states that "Members have an ethical obligation to weigh the societal benefits of their research against the costs and risks to human and animal welfare and impacts on the environment and society." The NSPE and AGI codes go somewhat further: "Engineers are encouraged to adhere to the principles of sustainable development in order to protect the environment for future generations," and "Geoscientists should strive to protect our natural environment. They should understand and anticipate the environmental consequences of their work and should disclose the consequences of recommended actions. They should acknowledge that resource extraction and use are necessary

  14. La actividad neotectónica en la sierra Baja de San Marcos - Cruz del Eje, flanco occidental de las Sierras Pampeanas Orientales

    A Massabie

    2003-12-01

    Full Text Available El área pedemontana de San Marcos Sierra-Cruz del Eje constituye, en la provincia de Córdoba, Argentina, una comarca clave para el análisis neotectónico en las Sierras Pampeanas Orientales. En esta región se han realizado estudios centrados en la unidad morfoestructural sierra Baja de San Marcos donde, aplicando un control múltiple de datos estructurales, geomorfológicos, sedimentológicos, paleontológicos y radimétricos, se ha determinado temporal y espacialmente la evolución de la actividad neotectónica del fallamiento andino. Se presentan evidencias sobre la reactivación cuaternaria tardía de la Falla Sierra Baja de San Marcos, conspicua falla inversa de vergencia occidental con componentes de desplazamiento de rumbo y de inclinación, enlazando los episodios tectónicos con las modificaciones del paisaje. Estas modificaciones son expresadas por los depósitos de endicamiento del río Quilpo, por los niveles aterrazados, vinculados a los cauces actuales de los ríos de la Costa (San Marcos y Quilpo, y por los cambios en su desarrollo de la red de avenamiento principal de la región. La edad 14C corregida de 2.560±120 aap, obtenida para los sedimentos del endicamiento permite acotar un primer episodio de actividad, Holoceno tardío, de la Falla Sierra Baja de San Marcos. Las observaciones efectuadas sobre la misma estructura, en una trinchera excavada para su investigación, confirman su extensión hacia el noroeste, dan evidencias sobre una segunda etapa de movimientos en el Holoceno tardío y determinan sus características estructurales comunes a otras fallas con actividad neotectónica estudiadas en las Sierras Pampeanas Orientales, las cuales responden a una mecánica transpresiva.

  15. H-index is important for postural control for people with impaired foot sole sensation.

    Shuqi Zhang

    Full Text Available People with Peripheral Neuropathy (PN, especially those with impaired sensory inputs through the small-afferent fiber (type II afferent fibers reflex loop (SAF, might depend more on the large-afferent fiber (type I afferent fibers reflex loop (LAF for postural control.To examine whether the function of the LAF reflex loop, reflected by the H-reflex and ankle joint proprioception, influences postural control when the SAF reflex loop is impaired, as indicated by reduced foot sole cutaneous sensation.Thirteen participants (8 women, 5 men diagnosed with PN and 12 age-matched controls (7 women, 5 men completed the testing protocol. Measures of interest included the H-index, active (AAP and passive (PAP ankle proprioception, plantar pressure sensitivity (PPS, average sway velocity (VAVG and area (A95 during 30 seconds eyes-closed standing, 6-minute walk distance (6MWD and timed up-and-go duration (TUG.Statistically significant group-dependent regression was observed between VAVG and H-index. Compared to the control group, the PN group demonstrated reduced PPS (2.0 ± 1.9 vs. 4.2 ± 1.2, P < .05 and H-index (63.6 ± 10.9 vs. 76.4 ± 16.0, P < .05, greater VAVG (3.5 ± 2.1 vs. 1.6 ± 0.6 cm/s, P < .05 and A95 (10.0 ± 10.1 vs. 2.5 ± 1.5 cm2, P < .05, shorter 6MWD (442.2 ± 93.0 vs. 525.3 ± 68.2 m, P < .05, and longer TUG (9.4 ± 1.6 vs. 6.5 ± 1.3 s, P < .05. Within the PN group, but not the control group, the H-index was correlated with VAVG (r = -.56, P < .05. Moreover, within the PN group only, PAP scores were correlated with 6MWD (r = -.68, P < .05 and TUG (r = -.59, P < .05 performance. No other statistically significant group difference, correlation or group-dependent regression was observed.VAVG, 6MWD, and TUG correlated with LAF reflex loop function observed among those with impaired functioning of the SAF reflex loop. This observation suggests that the LAF reflex loop may be critical to the control of balance in those individuals suffering

  16. Neonatal Intensive Care Unit Nurses’ Levels of Information Regarding Therapeutic Positions to be Applied to Preterm Newborns

    Diler Aydın

    2015-04-01

    Full Text Available Introduction: In order to provide optimal functionality of all the systems in the developmental care in the first days when they experience a great amount of stress in the neonatal intensive care unit (NICU, preterm newborns should be laid down in rather special positions. The basic aims of position management in the neonatal intensive care unit (NICU are to alleviate the pain/ache caused by the treatment/care interventions, minimise the effects of stressors resulting from the environmental factors and facilitate their coping with stress by preserving their postures. This study was planned to assess neonatal intensive care unit nurses’ levels of information regarding therapeutic positions to be applied to preterm newborns. Materials and Methods: The study was carried out with 52 neonatal nurses who worked in NICU in the city of Şanlıurfa and who accepted to participate in the study between September and December 2012 and also is a cross-sectional and descriptive study. Results: While 57.7% of the nurses who participated in the study stated that the fetus was in physiological flexion position in intrauterine period, only 11.5% of them stated that prone, supine and sidelying position are the therapeutic positions that could be applied to preterm babies in the NICU. As for the benefits of creating the correct position for the preterm babies in NICU the nurses stated that it increased the baby’s conditions for dealing with stress (32.7% and made external line-orientation easier (28.8%. As for the damages of positioning the preterm incorrectly, they stated that it caused motor and behavioural disorders (32.7% and permanent posture disorders (26.9%. 44.2% of the Nurses stated that during their stay in the intensive care units babies should be in the prone position as recommended by the American Paediatrics Academy (AAP and 25% of them stated that they should be in the supine position to prevent the risk of sudden infant mortality syndrome after

  17. 参加水中氨氮、挥发酚检测能力验证的体会%A Proficiency Test Report of Ammonia and Volatile Phenol in Water

    莫建芳; 黄闽燕; 陈一军

    2011-01-01

    [目的] 通过参加水中氨氮、挥发酚的检测能力验证,进一步总结经验,提高水质检测水平.[方法] 采用(GB/T5750.5-2006)纳氏试剂分光光度法测定氨氮、(GB/T5750.4-2006)氨基安替吡啉三氯甲烷萃取分光光度法测定挥发酚.以Z比分值来评价和判定检测结果.[结果] 本实验室氨氮测定数据实验室间Z比分值(ZB)为0.35,实验室内Z比分值(ZW)为0.22,挥发酚测定数据ZB值为-1.12,ZW值为0.54,|Z|均≤2,二项能力验证结果均为满意.[结论] 在本次水中氨氮、挥发酚能力验证活动中,通过采用标准样品进行实验室内部质量控制,严格按方法要求配制试剂和操作,用超纯水仪处理实验用水,控制空白值等关键点,可以获得满意结果.%[ Objective ] To further improve the water testing capability through a proficiency test of ammonia and volatile phenol in water. [ Methods ] The level of ammonia was tested by a Nessler spectrophotometry and the level of phenol was tested by a 4-AAP-chloroform extraction spectrophotometry. Z-score was used to evaluateand predicate test results. [ Results ] In the ammonia test, the Z-score of tests among laboratories( ZB )was 0.35 and the Z-score of tests within the same laboratory( ZW )was 0.22; in the phenol test, ZB was -1.12 and ZW was 0.54. It indicated that both tests met the proficiency requirement( both | Z | ≤2 ).[ Conclusion ] The proficiency tests suggestion couple of key issues in laboratory management, such as internal quality control procedure, reagents' preparation and operation, purified water and blank controls.

  18. A study of violent attitude on violent offenders%服刑人员暴力型罪犯的暴力态度研究

    关慕桢; 苗丹民; 李晓京; 田步忍; 张宝平; 段卫杰; 田京; 闫虎; 刘旭峰

    2010-01-01

    Objective To explore the structure of violent attitude and the predictive character of the passionate criminal and recidivist. Methods This study used FMMU-Abnormal Personality Risk Factors Inventory (FMMU-APRI) for measuring explicit violent attitude of the passionate criminal and recidivist in prison; while used the picture Attitude Activation paradigm (AAP) for assessing implicit violent attitude. Results The AAP results showed that parts of passionate criminal and recidivist had reversed priming effect, according to whether reversed, there were divided into four types: no-reversal passionate criminals, reversal passionate criminals, no-reversal recidivists and reversal recidivists, VIO scores of no-reversal passionate criminals and recidivists recidivist were significantly higher than the norm, as reversal passionate criminals and recidivists had no significance; in noreversal passionate criminals, compatible response rate was 1.104, incompatible response rate was 1.053; in reversal passionate criminals, compatible response rate was 1.042, incompatible response rate was 0.997; in no-reversal recidivists, compatible response rate was 1.059, incompatible response rate was 1.097; in reversal recidivists , compatible response rate was 1.039, incompatible response rate was 0.998, as each group had strong priming effect, and each group had a dissociation of implicit and explicit violent attitude. Conclusion The results suggest that offenders who have committed a crime in the same category also had a different violent attitude; and integrating indirect methods with direct methods would predict a crime more accurately.%目的 探讨激情犯和暴力型累惯犯的暴力态度的特点及预测特征.方法 试验采用FMMU变态人格危险性评估问卷测量在押激情犯和暴力型累惯犯的外显暴力态度;采用图片态度启动模式测量其内隐暴力态度.结果 结果显示部分罪犯出现了内隐态度的反转效应,据此分为激情犯未

  19. Ant Colony Algorithm based on the ASRank and MMAS for the Aircraft Assigning Problem%基于ASRank和MMAS的蚁群算法求解飞机指派问题

    张涛; 胡佳研; 李福娟; 张玥杰

    2012-01-01

    本文将航班串的飞机指派问题归结为车辆路径问题,考虑连续航班串之间衔接时间、衔接机场的约束、每架飞机的总飞行时间约束,建立了带有飞行时间约束的车辆路径问题的混合整数规划模型.构造了蚁群系统算法,引入基于排序的蚂蚁系统和最大最小蚂蚁系统算法的信息素更新策略.选取某航空公司7组初始航班串集合进行测试,并对算法中的重要参数进行了分析.实验结果表明,本文设计的模型和算法可以有效地减少连续航班串之间的总衔接时间,在可接受的计算时间内获得满意解.%The domestic airlines are relatively small in comparison with the international airlines. Airlines used to make the flight plans using simple and rough methods. The competition among the airlines became stronger with the expansion of airlines, and the opening of the air transportation market. Therefore, flight-planning management becomes more important. Aircraft assigning problem ( AAP) is to assign planes to the proper flight reasonably in order to make full use of the fleet resources. Good flight planning can not only ensure the safety and punctuality of flights, but also improve the utilization rale of fleets and decrease the cost of operation and maintenance so as to maximize the economic benefits of the airlines.The theoretical research on the flight-assigning problem is in its infancy, and most of the research just considers the next day aircraft assignment. However, in practice the airline engineering personnel generally makes aircraft plans every week. Although conducting aircraft assignment daily simplifies the problem and greatly reduces the constraints involved, the assignment method can hardly capture the actual demand. Based on the process of flight planning of the domestic airlines, we transform the aircraft assignment problem into vehicle routing problem (VRP) and study the weekly flight assignment problem. This problem is a kind

  20. Anestesia endovenosa en perros mediante el uso de propofol en dosis única, premedicado con acepromazina-atropina y xilazina-atropina Intravenous anaesthesia in dogs using a single dose of propofol premedicated with atropine _ acepromazine or atropine _ xylazine

    J. THIBAUT

    2002-01-01

    Full Text Available Se efectuó la valoración de la acción anestésica del propofol en perros, mediante administración endovenosa en dosis única, con dos protocolos de premedicación: atropina - acepromacina, y atropina - xilacina. Se trabajó con 20 perros mestizos de uno a seis años de edad, clínicamente sanos, divididos al azar en dos grupos de 10 cada uno. Al primer grupo, de 16.2±1.63 kg, se administró acepromacina en dosis de 1.5 mg/kg i.m. , al grupo 2, de 11.9±1.71 kg, se le aplicó xilacina en dosis de 3 mg/kg i.m. A ambos grupos se inyectó atropina en dosis de 0.1 mg/kg s.c. Se administraron 10 min. más tarde 5 mg/kg de propofol en la vena femoral, luego se evaluó el efecto del propofol sobre las variables: período de latencia, duración de la anestesia quirúrgica , período de recuperación anestésica, frecuencia respiratoria, frecuencia cardíaca, presión arterial y temperatura corporal. Además se determinaron las reacciones adversas. Los resultados difirieron significativamente entre los dos grupos. La inducción anestésica fue de 0.45 + 0.03 min. en el primero (A.A.P y 0.26 + 0.03 min. en el segundo (X.A.P. La duración de la anestesia quirúrgica fue de 12.3 + 1.89 min y 25.2 + 1.78 min y el período de recuperación de 4.5 + 0.63 min y 10,1 + 0,98 min en el grupo 1 y 2, respectivamente. Las variables fisiológicas en ambos grupos se mantuvieron sin modificaciones estadísticamente significativas durante el transcurso de la anestesia quirúrgica; la frecuencia respiratoria tuvo un valor inicial promedio de 14.3 + 2.45 y 13.0 + 1.54 ciclos/min. en el grupo 1 y 2, respectivamente. La frecuencia cardíaca fue de 175 + 11.81 lat/min en el grupo 1 y 148.4 + 9.04 lat/min en el grupo 2. La presión arterial promedio fue de 102.6 + 5.69 y 111.8 + 10.43 mm Hg para el grupo 1 y 2 respectivamente y la temperatura corporal en el grupo 1 fue de 38.5 + 0.17 y en el grupo 2 de 38.7 + 0.20 ºC. Las reacciones adversas se presentaron al inicio de la

  1. 布地奈德/福莫特罗吸入对哮喘患者血清基质金属蛋白酶-2、9水平的影响及疗效观察%Influence and curative effect of inhalation of formoterol/budesonide on matrix metalloproteinase of plasma-2 and 9 for asthmatic patients

    卢艳萍; 张天翼

    2014-01-01

    Objective To discuss the influence and curative effect observation of inhalation of formoterol/budesonide on matrix metalloproteinase of plasma(MMP)-2 and 9 for asthmatic patients (AAPs).Methods According to the digital table,68 cases of AAPs in acute stage of attack were randomly divided into observation group and control group.The patients in two groups were given routine medical treatments like anti-infection,antispasmodic and relieving asthma,relieving coμgh and reducing sputum and etc.In addition,the patients in observation group were given the inhalation of formoterol/budesonide with 4.5μg/160μg,two times daily for 2 weeks.Except for formoterol/budesonide,the medical treatments given to patients in control group were the same with those in observation group.The changes of MMP-2 and 9 levels in blood serum before and after 2 weeks' medical treatment were observed and compared,and proceed with the curative and safety observation.Results After 2 weeks' medical treatment,the MMP-2 and 9 in blood serum both were declined sharply(t =2.87,2.97,2.21,2.31,P < 0.01 or P < 0.05),and the compared with control group,the decrease value(t =2.45,2.37,all P < 0.05) was obviously higher.Meanwhile,the total clinical effective rate in observation group (94.12%) was obviously higher than that in control group (70.59%)(x2 =6.48,P < 0.05) ; The numbers of untoward effect occurrence in the medical treatment period were 3 cases in control group and 5 cases in observation group,which both had light symptom and did not appeared serious untoward effects.The comparison result of untoward effect between two groups had no obvious statistical difference(x2 =0.14,P > 0.05).Conclusion formoterol/budesonide has reliable medical treatment effect and high security to asthmatic,whose mechanism of action has effect on MMP-2 and 9 level adjustment,correction of degradation of extracellular matrix and dysequilibrium,and chronic airway inhibition and remodeling.%目的 探讨布地

  2. 巢湖水体-沉积物磷形态与有效性%Forms and Bioavailability of Phosphorus in Water and Sediments of Lake Chaohu

    龚莹; 王宁; 李玉成; 宗宁; 罗军; 谢毫

    2015-01-01

    level of eu⁃trophication. Among the five chemically extractable forms of P in the sediments, TP, Fe/Al⁃P (iron/aluminum bound P) and AAP ( algal⁃available P ) in the three parts of the lake followed an order of western part>eastern part>central part in content;OP ( organic P ) in an order of eastern part > western part > central part;Ca⁃P ( calcium bound P ) did not vary much between the parts;and DGT⁃P ( P determined with the DGT method) did not either within the lake, but did in the areas of river mouths, indicating that the areas of river mouths were greatly influenced by the pollutants carried by river flows. DGT⁃P in the sediments was positively related to highly active AAP, Fe/Al⁃P,OP and DIP, with coefficient R being 0�541, 0�547,0�731( P<0�01) and 0�438( P<0�05) , respectively, which indicates that the DGT device was effective in monitoring bio⁃available P in the sediments. Isothermal adsorption study indicates that the sediments in the western and eastern parts of the lake were both high in P adsorption potentiality, while those in the central part were quite low. Howev⁃er, DGT⁃P in equilibrium solutions varied sharply, with the highest equilibrium concentration of the sample from Sampling Site No. 9( 0�62 mg·L-1 ) in the western part of the lake being 9 times as high as that of the sample from Sampling Stie No. 3( 0�07 mg·L-1 ) in the eastern part of the lake, suggesting that sediments may influence, to a quite extent, bio⁃a⁃vailability of P in the overlaying water.

  3. Dissolved and acid available particulate beryllium in eastern UK surface waters.

    Neal, Colin

    2003-10-01

    , particulate nitrogen, particulate phosphorus, and the AAP for several transition metals (Al, Co, Cr, Fe, Mn, Ni and Y), and the lanthanides. This reflects the nature of the catchment sources of particulate material (soil erosion and pollutant sources) and the hydrogeochemistry of Be with the high potential for hydrolysis and hydrogen bonding due to its small atomic size and high surface charge density. The acid available particulate flux averages approximately 0.42 gha(-1) year(-1) with a range in mean across the sites of 0.14-1.2 gha(-1) year(-1). The highest flux occurs for a river with a flood plain contaminated by spoil from historic mining of lead-zinc deposits, but there is no clear separation between the rural and the industrial/urban impacted catchments. The results indicate a potential and most unexpected dissolved Be stress to lowland aquatic environments, a stress that might well increase over time for the UK given the increasing climate instability within the country. The findings are linked to other information on Be in surface waters for other UK surface waters, including previously unpublished data, to allow a broader perspective for UK riverine environments. Information is provided on Be levels in acidic upland streams in mid Wales (upper River Severn), other eastern UK rivers (collected towards the end of the LOIS initiative) and drainage waters from a disused tin wine in Cornwall (Wheal Jane Mine). PMID:14499533

  4. Enhanced Web-Based Otitis Study Case vs. Simple Paper-Case: Impact on Medical Student Objective Structured Clinical Exam Performance

    Michael H. Malloy, M.D., M.S.

    2002-01-01

    Full Text Available Background: Distance education methods have taken on greater importance as medical student education has moved off campus into the community. What the best methods are for conveying information to students at distant sites has not been determined. Objective: To determine if students at distant community sites who received an otitis media study case by e-mail that was enhanced with a referral to a web-based otitis study case, performed better on otitis OSCE stations than students who received the same case not enhanced with visuals or referrals to a web-based otitis case. Design/Methods: Students were randomized by community site to receive either the enhanced (E or simple otitis study case (S. Students were e-mailed an otitis media study case during the 5th week of the rotation. Those randomized to the E-case received a case that started with a case scenario followed by a "Task" that instructed them to go to this web address: http://www.aap.org/otitismedia/www/vc/ear/index.cfm (American Academy of Pediatrics Otitis Web Site. They were then to select "Case 1" which was a continuation of the case scenario present on their e-mail. A list of learning objectives was also printed on the e-mail. Students receiving the S-case viewed the same case scenario and objectives, but were not instructed to go to the web page. All students rotated through two OSCE otitis stations. In the first station they interviewed a simulated patient(OSCE-SP and counseled her on the management of her 12 month old with otitis. Within that station they viewed a video of a pneumoscopic exam of two ears, one ear with otitis and the other ear normal. At the 2nd otitis station the student presented the case to a faculty and was asked a series of questions about otitis media(OSCE-PR. Scores on the two stations were compared by group. Results: There were 198 students who took the OSCE. 178 (90% responded to a survey that indicated they had opened and read the e-mailed case. There

  5. Where to Look for Habitability

    Kohler, Susanna

    2016-03-01

    photometrically derived stellar parameters agree well with the parameters in EDE, typically measured spectroscopically.Providing Survey TargetsPlot showing the number of stellar habitable zones that can be probed by a survey, based on how long the stars are observed in the surveys. Surveys listed as reference points are TESS at 27 days, K2 at 75 days, PLATO at 180 days, HARPS at 6 years, and AAPS at 15 years. [Chandler et al. 2016]The final CELESTA catalog details the habitable zones of 37,354 bright, main-sequence stars. The stars habitable-zone widths are generally under 5 AU, with the majority falling between 1 and 1.5 AU. The authors also provide an estimate of how many of these habitable zones current surveys (like Kepler) and upcoming surveys (like the Transiting Exoplanet Survey Satellite, or TESS) will be able to probe, based on the duration of the surveys typical campaigns.Though a planets potential for habitability relies on additional factors besides the location of its orbit, cataloging the locations of stellar habitable zones for nearby, observable stars is an important start. CELESTA is an excellent reference for this, and it will provide a living resource that the authors plan to continue to update with additional stars, as well as with improved-accuracy stellar measurements, expected from upcoming astrometric missions.CitationColin Orion Chandler et al 2016 AJ 151 59. doi:10.3847/0004-6256/151/3/59

  6. Development of female medfly, Ceratitis capitata (Wiedemann) attractant system for trapping and sterility assessment: investigations of the efficiency of various medfly female trapping combinations in the western part of Turkey in support of the sterile insect technique

    Fourteen combinations, formed from eight traps including some of their versions and seven lures and attractants, were tested between the years of 1994-1997. The traps tested were: Jackson trap (JT); International Pheromone's McPhail traps (IPMT); Yellow and white bottomed, Closed-bottom dry trap (CBDT); Open-bottom dry trap (OBDT); Agrisense dry trap; Tephri trap; and Frutect trap. The lures included: ammonium acetate (AA) plus putrescine (P), the FA-2 lure; AA+P+ trimethylamine (TMA), the FA-3 lure; NuLure and borax (NU+B); a special liquid lure; and Trimedlure (TML). For killing agents, either toxicant squares of DDVP or the surfactant Triton were used. Each experiment per year generally consisted of two independent tests that lasted eight weeks. The field plot design was linear or mostly randomized block design. Fruit infestation level was estimated for each test. Mating status of the captured females was also studied. The assessment was based on the number of adult captured. Singe the yearly experiments were not based on the same treatments, some combinations were eliminated or modified after testing. In 1994, the CBDT baited with FA-2 was tested against JT, TML and seemed almost as attractive as JT with the percentage of 61% - 62% females. A modified trap, the OBDT was tested in 1995 along with IPMT, NU+B and Agrisense drytrap, FA-2 and they showed the weakest capture efficiencies. In 1996 when the OBDT and IPMT were tested with FA-2 and FA-3 lures, the traps with FA-3 showed better performance than the same traps with FA-2 (4.07 vs 1.96 and 10.32 vs 3.04 flies/trap/day (F/T/D) respectively). The Tephri trap, which was first tested with NU+B, had best capture efficiency results when used with DDVP plug. The Frutect trap with its own liquid lure gave the weakest result followed by OBDT, FA-2. In 1997, the Tephri, FA-3,wet, in both tests, seemed to be the most attractive treatment with 14.14 and 3.96 F/T/D followed by Tephri, FA-3,dry with 12.37 and 2.63 F

  7. Design of ABC damage variable and positioning system for acetabular fractures and 1122 cases of multi-center statistic analysis%髋臼骨折ABC损伤变数定位系统的设计与1122例多中心研究分析

    张春才; 苏佳灿; 曹烈虎; 徐永清; 阮墨; 陈庄洪; 黄继峰; 蔡贤华; 沈惠良; 刘利民; 王继芳; 许硕贵; 王岩; 唐佩福; 梁雨田; 王家让; 王愉思; 王振昊; 刘文德; 李文锐; 李文虎; 王序全; 禹宝庆; 周东生; 张鹏; 王仁; 王刚; 陈育岳; 丛永健; 纪方; 付青格; 刘欣伟; 章云童; 牛云飞; 王攀峰

    2011-01-01

    1026 cases (90%). Acetabular variables:variabe 1 in 203 cases of simple displaced fracture ( 17.8% ) ;variabe 2 in 516 cases of comminuted fracture (45.3%) ;variabe 3 in 421 cases of compression fracture (36.9%); 249 cases of pelvic ring lesions damage (21.8% ), 75 cases femoral head fracture (6.6%); 18 cases of double side acetabular fracture and relative pelvic ring and proximal humerus joint variables (1.58%). Key part and curative effect elements of 1140 cases acetabular fracture:507 cases of dome or posterior acetabular fracture (44.5%); 421 cases of compression fracture (36.9%) ;249 cases of pelvic ring variables (21.8%); 75 cases of proximal humerus joint variables (6.6%);486 cases of simple Aa/p1/2,Bapl/2 acetabular fracture (42.6%). Conclusion:Compression fracture,especially defected compression fracture,takes important part in acetabular damage variables, and also presents that acetabular fracture with pelvic ring and proximal femoral damage variables are not rare at all. The relationship of the acetabular fracture damage variables,and its percentage shows the key points and elements in clinical treatment:weight-beating to dome accounts for 44.5% ;compression to defects account for 36.9% ,pelvic ring to float accounts for 21.8% ;dome fracture to double side fracture account for 6.6%. The system has significant guiding effects on clinic in terms of evaluation of injury severity ,anatomic localization, difficulty index, alternative strategy, operative approach, effect of treatment, and prognosis. And the most important thing is that the system creates the comparison of damage variables in same type of fracture and the communication of homo-language and explores a new method.%目的:设计髋臼骨折ABC损伤变数定位系统并通过1122例髋臼骨折多中心分析研究,探索该系统的可行性与临床实用价值.方法:根据髋臼三柱壁概念,参考髋臼骨折多米诺骨牌效应所致的骨盆环损伤方向和

  8. Estudo retrospectivo de 47 complicações em 380 pacientes operados de câncer retal

    Geraldo Magela Gomes da Cruz

    2006-06-01

    Full Text Available No decurso de 31 anos de prática coloproctológica, acumulando um fichário com 24.200 pacientes, 923 (3,8% portadores de câncer no intestino grosso, dos quais 870 eram adenocarcinomas colorretais e 53 tumores malignos de ânus e margem de ânus. Dos 870 casos de adenocarcinomas colorretais, 490 (56,3% localizavam-se nos cólons e 380 (43,7% no reto. O presente trabalho tem como objetivo principal analisar uma casuística de 380 pacientes portadores de câncer retal atendidos de 1965 a 1996, 373 dos quais operados, com ênfase às técnicas cirúrgicas usadas e às 47 complicações decorrentes das cirurgias e suas correções. O CR foi mais comum no terço inferior (172; 45,3%, seguindo o terço superior (126; 33,1% e o médio (82; 21,6%. A ressecabilidade dos tumores retais de terço superior foi de 88,9% (112 pacientes, e a retossigmoidectomia abdominal com anastomose manual foi a cirurgia mais praticada (76 pacientes; 60,3%. A ressecabilidade dos tumores retais de terço médio foi de 90.2% (72 pacientes, e a retossigmoidectomia abdominal com anastomose manual e mecânica foi a cirurgia mais praticada (40 pacientes; 48,7%. A ressecabilidade dos tumores retais de terço inferior foi de 89,5% (154 pacientes, e a amputação abdominoperineal com colostomia definitiva foi a cirurgia mais praticada (154 pacientes; 74,4%. A ressecabilidade tumoral foi elevada (338 pacientes; 88,9%. As cirurgias mais praticadas foram a AAP (135 ou 35,5% e a retossigmoidectomia abdominal com anastomose colorretal manual e mecânica (130; 34,3%. A ocorrência de complicações cirúrgicas imediatas maiores foi de 12,6% (47 em 380 casos; e a mortalidade cirúrgica foi de 1,6% (6 casos. As 2 cirurgias mais praticadas apresentaram índices de complicações consideradas baixos: amputação abdominoperineal à Miles com colostomia definitiva - 135 cirurgias com 13 complicações (9,6% e 2 óbitos (1,5%; e a retossigmoidectomia abdominal tipo Dixon com anastomose manual

  9. Guidelines for the Prevention and Treatment of Opportunistic Infections among HIV-exposed and HIV-infected children: recommendations from CDC, the National Institutes of Health, the HIV Medicine Association of the Infectious Diseases Society of America, the Pediatric Infectious Diseases Society, and the American Academy of Pediatrics.

    Mofenson, Lynne M; Brady, Michael T; Danner, Susie P; Dominguez, Kenneth L; Hazra, Rohan; Handelsman, Edward; Havens, Peter; Nesheim, Steve; Read, Jennifer S; Serchuck, Leslie; Van Dyke, Russell

    2009-09-01

    endorsement by NIH, CDC, the HIV Medicine Association (HIVMA) of the Infectious Diseases Society of America (IDSA), the Pediatric Infectious Disease Society (PIDS), and the American Academy of Pediatrics (AAP). The recommendations are rated by a letter that indicates the strength of the recommendation and a Roman numeral that indicates the quality of the evidence supporting the recommendation so readers can ascertain how best to apply the recommendations in their practice environments. An important mode of acquisition of OIs, as well as HIV infection among children, is from their infected mother; HIV-infected women coinfected with opportunistic pathogens might be more likely than women without HIV infection to transmit these infections to their infants. In addition, HIV-infected women or HIV-infected family members coinfected with certain opportunistic pathogens might be more likely to transmit these infections horizontally to their children, resulting in increased likelihood of primary acquisition of such infections in the young child. Therefore, infections with opportunistic pathogens might affect not just HIV-infected infants but also HIV-exposed but uninfected infants who become infected by the pathogen because of transmission from HIV-infected mothers or family members with coinfections. These guidelines for treating OIs in children therefore consider treatment of infections among all children, both HIV-infected and uninfected, born to HIV-infected women. Additionally, HIV infection is increasingly seen among adolescents with perinatal infection now surviving into their teens and among youth with behaviorally acquired HIV infection. Although guidelines for postpubertal adolescents can be found in the adult OI guidelines, drug pharmacokinetics and response to treatment may differ for younger prepubertal or pubertal adolescents. Therefore, these guidelines also apply to treatment of HIV-infected youth who have not yet completed pubertal development. Major changes in the

  10. ”Fra passivitet til aktivitet” – En oversikt over utvikling og endringer på folketrygdens områder de senere årene

    Grete Damberg

    2010-01-01

    the national insurance scheme. The importance of individual activity has been more and more emphasized in cases where there are no medical obstacles. The changes that have been made focus on the individual's opportunity to work actively rather than possible limitations. This manifests itself through changes in the field of sickness absence with the introduction of compulsory activity for the persons on sick leave, as well as a new sickness absence model focussing on follow-up, involvement of employers, the medical practitioner and The National Labour and Welfare Administration (NAV. The work-place is the most suitable arena for follow-up of sickness absence, and employer and employee are the most important participants. This is a continuation of the Letter of intent on a more inclusive working life (IW. Also within the fields of rehabilitation allowance, vocational rehabilitation and disability benefits emphasis is placed on the individual's willingness to try working if medically feasible. The year 2010 will see extensive modifications of rehabilitation allowance, vocational training and time limited disability benefit, which all will be replaced by "work ability assessment benefit" (AAP, a benefit which also emphasizes activity. At the same time a special assessment of work ability will be introduced as a working method internally in NAV. In the field of occupational injury there are few changes, but the organisation of the occupational injury scheme is under revision. Cooperation with medical practitioners and offers of training has been intensified during the past few years. NAV has also developed statistics over sick leave issued by general practitioners. This tool will be used in dialogue with the general practitioner. Still, many persons are on sick leave and long term benefits. The reasons for this are complex. Further research is needed within the field of national insurance.

  11. Screening and brief intervention for alcohol and other abuse.

    Harris, Sion Kim; Louis-Jacques, Jennifer; Knight, John R

    2014-04-01

    Substance use is the most common health risk behavior among adolescents and is one of the greatest threats to their current and future health. Universal screening of adolescents in general medical settings can be instrumental in identifying substance use early, before further problems develop and when BIs are more likely to be effective. Screening in and of itself may have some therapeutic effect. Brief screening tools feasible for use by busy medical offices to quickly and reliably assess adolescent risk for a substance use disorder now are available. A recent study found that a physician-conducted CRAFFT screen interview required an average of 74 seconds to complete, whereas a computer self-administered version took an average of 49 seconds. The CRAFFT and AUDIT tools currently have the most evidence for validity among adolescents, whereas the validity of other widely used tools such as DAST-10, NIDA-modified ASSIST (Alcohol, Smoking and Substance Involvement Screening Test), and ultra-brief screens (AUDIT-C, single-item screens) has yet to be established for adolescents. Studies are needed to identify effective strategies to promote universal adolescent screening and the use of valid screening tools in general medical settings. One statewide (Massachusetts) study found that although most (86%) primary care physicians seeing adolescents reported screening adolescents for substance use annually, only 1 in 3 reported using a validated tool (the CRAFFT). The remaining physicians reporting using informal screening procedures, their own questionnaire, or the CAGE. Computerization of screening and integration into the electronic health record appear to be promising strategies to promote universal screening and standardized use of valid screening tools. Increasing adolescent screening rates necessitates supporting physicians' ability to respond effectively to the screen results. To that end, recent evidence-informed practice guides from the AAP and NIAAA provide a

  12. Frequency of Meningitis in Children Presenting with Febrile Seizures at Ali- Asghar Children’s Hospital

    Azita TAVASOLI

    2014-12-01

    -69.Hom J, Medwid K, The low rate of bacterial meningitis in children, ages 6 to 18 months, with simple febrile seizures. AcadEmerg Med 2011; 18(11:1114-1120.Fetveit A. Assessment of febrile seizures in children. Eur J Pediatr 1998; 167(1; 17-27.Subcommittee on Febrile Seizures. Febrile seizures: Guidelines for the neurodiagnostic evaluation of the child with a simple febrile seizure. Pediatrics 2011; 127(2:389-394.Rosman NP. Evaluation of the child who convulses with fever. Pediatr Drugs 2003; 5(7:457- 461.Dubos F, De la Rocque F, Levy C et al. Sensitivity of the bacterial meningitis score in 889 children with bacterial meningitis. J Pediatr 2008; 152:378-382.Nigrovic LE, Kuppermann N, Macias CG, et al. Clinical prediction rule for identifying children with cerebrospinal fluid pleocytosis at very low risk of bacterial meningitis. JAMA 2007; 297(1:52-60.American Academy of Pediatrics. Provisional Committee on quality improvement, Subcommittee on Febrile Seizures. Practice parameter: the neurodiagnostic evaluation of the child with a first simple febrile seizure. Pediatrics 1997; 769-772.Najaf-Zadeh A, Dubos F, Hue V, et al. Risk of bacterial meningitis in young children with a first seizure in the context of fever: a systematic review and meta- analysis. PLoS One. 2013; 8(1:e55270. Doi: 10. 1371/journal. pone.0055270.Owusu-Ofori A, Agbenyega T, Ansong D, et al. Routine lumbar puncture in children with febrile seizures in Ghana: should it continue? International Journal of Infectious Diseases 2004; 8:353-361.Kimia AA, Capraro AJ, Hummel D, Johnston P, Harper MB. Utility of lumbar puncture for first simple febrile seizure among children 6 to 18 months of age. Pediatrics 2009; 123(1: 6–12.Shaked O, Pena BM, Linares MY, Baker RL. Simple febrile seizures: are the AAP guidelines regarding lumbar puncture being followed? Pediatr Emerg Care 2009; 25(1:8-11.Seltz LB, Cohen E, Weinstein M. Risk of bacterial or herpes simplex virus meningitis/encephalitis in children with complex

  13. Family pediatrics: report of the Task Force on the Family.

    Schor, Edward L

    2003-06-01

    female-headed families than for married-couple families. The comped families than for married-couple families. The composition of children's families and the time parents have for their children affect child rearing. Consequent to the increase in female-headed households, rising economic and personal need, and increased opportunities for women, the proportion of mothers who are in the workforce has climbed steadily over the past several decades. Currently, approximately two thirds of all mothers with children younger than 18 years are employed. Most families with young children depend on child care, and most child care is not of good quality. Reliance on child care involves longer days for children and families, the stress imposed by schedules and created by transitions, exposure to infections, and considerable cost. An increasing number and proportion of parents are also devoting time previously available to their children to the care of their own parents. The so-called "sandwich generation" of parents is being pulled in multiple directions. The amount and use of family time also has changed with a lengthening workday, including the amount of commuting time necessary to travel between work and home, and with the intrusion of television and computers into family life. In public opinion polls, most parents report that they believe it is more difficult to be a parent now than it used to be; people seem to feel more isolated, social and media pressures on and enticements of their children seem greater, and the world seems to be a more dangerous place. Social and public policy has not kept up with these changes, leaving families stretched for time and stressed to cope and meet their responsibilities. What can and what should pediatrics do to help families raise healthy and well-adjusted children? How can individual pediatricians better support families? FAMILY PEDIATRICS: The American Academy of Pediatrics (AAP) Board of Directors appointed the Task Force on the Family to

  14. Familial Hemiplegic Migraine and Spreading Depression

    Hadi KAZEMI

    2014-07-01

    mutations. Neurology 2009 31;72(13:1178-83.Thomsen LL, Ostergaard E, Olesen J, Russell MB. Evidence for a separate type of migraine with aura: sporadic hemiplegic migraine. Neurology 2003;60(4:595-601.van den Maagdenberg AM, Haan J, Terwindt GM, Ferrari MD. Migraine: gene mutations and functional consequences. Curr Opin Neurol 2007; 20:299–305.Gritz SM, Radcliffe RA. Genetic effects of ATP1A2 in familial hemiplegic migraine type II and animal models. Hum Genomics 2013;5;7:8.Ophoff RA, Terwindt GM, Vergouwe MN, et al. Familial hemiplegic migraine and episodic ataxia type-2 are caused by mutations in the Ca2+ channel gene CACNL1A4. Cell 1996;87:543–552.Gargus JJ, Tournay A. Novel Mutation Confirms Seizure Locus SCN1A is Also Familial Hemiplegic Migraine Locus FHM3.PediatrNeurol 2007;37:407-410.Tottene A, Conti R, Fabbro A, Vecchia D, Shapovalova M, Santello M, et al.Enhanced excitatory transmission at cortical synapses as the basis for facilitated spreading depression in Ca(v2.1 knockin migraine mice. Neuron 2009;61(5:762-73.Terwindt GM, Ophoff RA, Haan J, Sandkuijl LA, Frants RR, Ferrari MD. Migraine, ataxia and epilepsy: a challenging spectrum of genetically determined calcium channelopathies. Dutch Migraine Genetics Research Group. Eur J Hum Genet 1998;6(4:297-307.Terwindt GM, Ophoff RA, van Eijk R, Vergouwe MN, Haan J, Frants RR, et al. Dutch Migraine Genetics Research Group. Involvement of the CACNA1A gene containing region on 19p13 in migraine with and without aura. Neurology 2001;56(8:1028-32.Franceschini A, Vilotti S, Ferrari MD, van den Maagdenberg AM, Nistri A, Fabbretti E. TNFα levels and macrophages expression reflect an inflammatory potential of trigeminal ganglia in a mouse model of familial hemiplegic migraine. PLoS One 2013;8:e52394.Kodangattil JN, Möddel G, Müller, M, Weber W, Gorji A.The inflammatory chemokine CXCL10 modulates synaptic plasticity and neuronal activity in the hippocampus. European Journal of Inflammation 2012;10(3:311-328Leao AAP

  15. Resúmenes del Segundo Congreso Colombiano y Segundo Simposio Latinoamericano, Iberoamericano y Colombiano sobre Gastroenterología, Hepatología y Nutrición Pediátrica

    2007-03-01

    ía. Independiente de la consulta médica, se realizó la nutricional para la cual no se conoció diagnóstico y se indicó a paciente y familiares no comentar al respecto. De la primera se registraron edad, peso, talla y diagnóstico. En la segunda, con recordatorio de 24 horas y módulos de alimentos, se estableció frecuencia y cantidad del consumo de alimentos, agua y otros líquidos. Se analizó FD basándose en datos de CYTED y comparando con sugerencia de AAP. Para el análisis los grupos fueron: a estreñimiento (n=30; b estreñimiento en mejoría (n=13; c colon irritable (n=12; d dolor abdominal recurrente (n=4; e dispepsia sin estreñimiento (n=20; f dispepsia con estreñimiento (n=5; g otros. También se analizaron clasificándolos en estreñidos (a,c,f y no estreñidos (b,d,e,g. No se encontraron diferencias significativas (Chi2 de Pearson en cuanto al consumo de FD, el cual fue adecuado o alto en 59%. La cantidad de líquidos en relación con FD fue baja en 95%. Conclusión: Independiente del diagnóstico los niños consumen cantidades similares de fibra, adecuadas en más de la mitad de ellos. El consumo de líquidos proporcional es bajo en la mayoría.Conocimientos, actitudes y prácticas relacionados con la lactancia materna en mujeres de edad fértil en una población vulnerable, Girón, ColombiaGamboa EM, López N, Prada GE, Gallo KJ Universidad Industrial de Santander, Bucaramanga, ColombiaIntroducción: La leche materna representa el mejor alimento para los niños hasta los seis meses de edad. Los estudios de conocimientos, actitudes y prácticas (CAP, han sido utilizados en investigación y pueden servir para la creación de estrategias de promoción de la salud y prevención de la enfermedad. Objetivo: Identificar los conocimientos, actitudes, prácticas relacionados con la lactancia materna en mujeres en edad fértil residentes del asentamiento las Marías del municipio de Girón en el año 2005. Materiales y métodos: Se realizó un corte transversal