WorldWideScience
1

Genetic Control of Capsid Length in Bacteriophage T4 I. Isolation and Preliminary Description of Four New Mutants  

UK PubMed Central (United Kingdom)

Four new mutants are described whose phenotypic expression affects the length of the head of bacteriophage T4D. All mutants produce some phenotypically normal phage particles. Mutant pt21-34...Full Text Available

1973-08-01

2

Primary study on lesion mimic mutants of rice (oryza sativa L.)  

International Nuclear Information System (INIS)

Nineteen lesion mimic mutants (xsl1-19) of japonica rice Xiushui11 were obtained by ?-rays irradiation treatment. All mutants belonged to whole life lesion mimic. Lesion mimic of mutants didn't largen after tillering stage, leaves didn't wither, and no effect on the plants exsert spikes and seed. When the highest temperature in day exceeded 32 degree C in seedling stage, lesion mimic of all mutant expect xsl19 disappeared. Under 32 degree C, lesion mimic would appear gradually, and symptoms weren't inhibited by high temperature after 5 leaf stage. The plant heights of all lesion mimic mutants were 47.56-63.54 cm in the tillering stage, and that of CK was 83.75 cm; but the dwarf phenomenon of mutants only appeared before tillering stage, and didn't affect plant heights finally; the heading dates of mutants were the same ...

2007-08-01

3

Evaluation of some lupin mutants under different irrigation intervals and nitrogen fertilizer levels  

International Nuclear Information System (INIS)

This investigation was carried out during the two seasons of 1998/1999 and 1999/2000 at the experimental farm belonging to the plant research department, nuclear research center, atomic energy authority in Inshas to evaluate four lupin mutant lines (L_1, L_2, L_3 and L_4) along with their original mother varieties giza 1 and giza 2 under different levels and nitrogen fertilizer levels. The results indicated that seed yield/ plant and its effective components number and weight of pods/plant were significantly higher in all the mutant lines as compared to their parents either in the first or second seasons. Concerning the effect of irrigation intervals on yield and its components for developed mutants and their mother varieties as well as the results gave an evidence that the wider interval showed the lesser values for yield and most of yield components. Obtained data also revealed that yield attributes of lupin ...

2002-04-01

4

Vibrational spectroscopy of bacteriorhodopsin mutants: light-driven proton transport involves protonation changes of aspartic acid residues 85, 96, and 212  

Energy Technology Data Exchange (ETDEWEB)

Fourier transform infrared (FTIR) difference spectra have been obtained for the bR----K, bR----L, and bR----M photoreactions in bacteriorhodopsin mutants in which Asp residues 85, 96, 115, and 212 have been replaced by Asn and by Glu. Difference peaks that had previously been attributed to Asp COOH groups on the basis of isotopic labeling were absent or shifted in these mutants. In general, each COOH peak was affected strongly by mutation at only one of the four residues. Thus, it was possible to assign each peak tentatively to a particular Asp. From these assignments, a model for the proton-pumping mechanism of bR is derived, which features proton transfers among Asp-85, -96, and -212, the chromophore Schiff base, and other ionizable groups within the protein. The model can explain the observed COOH peaks in the FTIR difference spectra of bR photointermediates and could also account for other recent results on ...

1988-11-15

5

Evaluation of radiation induced sesame mutants as affected by some micro nutrients  

International Nuclear Information System (INIS)

Tow experiments were carried out in 1981 and 1982 growth seasons at the greenhouse of the department of agriculture for soils and water researches, atomic energy authority, at inshas, to investigate the responses of two mutation derived lines of sesame and the local cultivar giza 25 to the application of micro nutrients. The possible effect of radiation on germination and growth of sesame seed was also studied in a separate experiment conducted in 1985 season, at inshas.

6

The effects of chemicals in the presence of cellophane on X-ray-induced point mutation and gene conversion in Aspergillus midulans  

International Nuclear Information System (INIS)

The presence of washed or unwashed cellophane alone or together with a bleomycin, mitomycin C or hydrochlorothiazide, ('Esidrex') showed no appreciable effect on survival of either unirradiated or irradiated conidia. Irradiation for a period of 20min reduced the survival of conidia to 20%. The growth of irradiated conidia in the presence of bleomycin, mitomycin C or Esidrex is associated with a 2- to 3-fold increase in the frequency of gene convertants, but was not accompanied by an increase in point mutants. When conidia were grown on cellophane but otherwise treated as before the frequency of gene convertants was increased 8-fold, but induction of point mutants was negligible. This effect was the same for irradiated and unirradiated conidia. The environment created by the cellophane in contract with the medium appears to affect the action of each of the three compounds synergistically. (author).

7

Mutations at the cysteine codons of the recA gene of Escherichia coli  

International Nuclear Information System (INIS)

Each of the three cysteine residues in the Escherichia coli RecA protein was replaced with a number of other amino acids. To do this, each cysteine codon was first converted to a chain-terminating amber codon by oligonucleotide-directed mutagenesis. These amber mutants were then either assayed for function in different suppressor strains or reverted by a second round of mutagenesis with oligonucleotides that had random sequences at the amber codon. Thirty-three different amino acid substitutions were obtained. Mutants were tested for three functions of RecA: survival following UV irradiation, homologous recombination, and induction of the SOS response. It was found that although none of the cysteines is essential for activity, mutations at each of these positions can affect one or more of the activities of RecA, depending on the particular amino acid substitution. In addition, the cysteine at position 116 appears to be ...

8

Effects of chemicals in the presence of cellophane on X-ray-induced point mutation and gene conversion in Aspergillus midulans  

Energy Technology Data Exchange (ETDEWEB)

The presence of washed or unwashed cellophane alone or together with a bleomycin, mitomycin C or hydrochlorothiazide, ('Esidrex') showed no appreciable effect on survival of either unirradiated or irradiated conidia. Irradiation for a period of 20min reduced the survival of conidia to 20%. The growth of irradiated conidia in the presence of bleomycin, mitomycin C or Esidrex is associated with a 2- to 3-fold increase in the frequency of gene convertants, but was not accompanied by an increase in point mutants. When conidia were grown on cellophane but otherwise treated as before the frequency of gene convertants was increased 8-fold, but induction of point mutants was negligible. This effect was the same for irradiated and unirradiated conidia. The environment created by the cellophane in contract with the medium appears to affect the action of each of the three compounds synergistically.

1984-08-01

9

Construction and evaluation of a double mutant of Shigella flexneri as a candidate for oral vaccination against shigellosis.  

Science.gov (United States)

Based on studies on the genetic and molecular basis of Shigella flexneri invasive properties, we have constructed and evaluated a double mutant of S. flexneri serotype 5 for utilization as a live attenuated oral vaccine against shigellosis. The first mutation, icsA, blocks intracellular spread of bacteria as well as cell-to-cell infection. It affects the capacity of the invasive pathogen to form large abscesses in epithelia. The second mutation, iuc, eliminates production of the siderophore aerobactin thus impairing growth of the bacterium within tissues. This double mutant, SC5700 appeared safe when administered intragastrically to macaque monkeys as three doses (5 x 10(10) c.f.u. each) at weekly intervals. Protection against a challenge by the wild type isolate (M90T) was observed 4 weeks after the last vaccine inoculation. Duration of carriage was considerably reduced as compared to the control group in which all animals ...

1989-10-01

10

Effect of mutations in HNF-1#alpha# and HNF-1#beta# on the transcriptional regulation of human sucrase-isomaltase in Caco-2 cells  

International Nuclear Information System (INIS)

Mutations in transcription factors hepatocyte nuclear factors (HNF)-1#alpha# and HNF-1#beta# cause maturity-onset diabetes of the young (MODY) types 3 and 5, respectively. HNF-1#alpha# and HNF-1#beta# mutations are well studied in some tissues, but the mechanism by which HNF-1#alpha# and HNF-1#beta# mutations affect sucrase-isomaltase (SI) transcription in the small intestine is unclear. We studied the effects of 13 HNF-1#alpha# mutants and 2 HNF-1#beta# mutants on human SI gene transcription, which were identified in subjects with MODY3 and MODY5, respectively. Transactivation activity of 11 HNF-1#alpha# and 2 HNF-1#beta# mutants was significantly lower than that of wild (wt)-HNF-1#alpha# and wt-HNF-1#beta#. Furthermore, in co-expression studies with mutant (mu)-HNF-1#alpha#/ wt-HNF-1#beta# and wt-HNF-1#alpha#/mu-HNF-1#beta#, the combination of mu-HNF-1#alpha# (P379fsdelCT and ...

2004-12-03

11

Transcriptional Profiling Analysis of the Global Regulator NorG, a GntR-Like Protein of Staphylococcus aureus.  

Science.gov (United States)

The GntR-like protein NorG has been shown to affect Staphylococcus aureus genes involved in the resistance to quinolones and ?-lactams, such as those encoding the NorB and AbcA transporters. To identify the target genes regulated by NorG, we carried out transcriptional profiling assays using S. aureus RN6390 and its isogenic norG::cat mutant. Our data showed that NorG positively affected the transcription of global regulators mgrA, arlS, and sarZ. The three putative drug efflux pump genes most positively affected by NorG were the NorB efflux pump (5.1-fold), the MmpL-like protein SACOL2566 (5.2-fold), and the BcrA-like drug transporter SACOL2525 (5.7-fold). The S. aureus predicted MmpL protein showed 53% homology with the MmpL lipid transporter of Mycobacterium tuberculosis, and the putative SACOL2525 protein showed 87% homology with the bacitracin drug transporter BcrA of Staphylococcus hominis. Two ...

2011-09-01

12

Roles of the #beta# 146 histidyl residue in the molecular basis of the Bohr Effect of hemoglobin: A proton nuclear magnetic resonance study  

International Nuclear Information System (INIS)

Assessment of the roles of the carboxyl-terminal #beta#146 histidyl residues in the alkaline Bohr effect in human and normal adult hemoglobin by high-resolution proton nuclear magnetic resonance spectroscopy requires assignment of the resonances corresponding to these residues. By a careful spectroscopic study of human normal adult hemoglobin, enzymatically prepared des(His146#beta#)-hemoglobin, and the mutant hemoglobins Cowtown (#beta#146His #-># Leu) and York (#beta#146His #-># Pro), the authors have resolved some of these conflicting results. By a close incremental variation of pH over a wide range in chloride-free 0.1 M N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid buffer, a single resonance has been found to be consistently missing in the proton nuclear magnetic resonance spectra of these hemoglobin variants. The results indicate that the contribution of the #beta#146 histidyl residues is 0.52 H"+/hemoglobin tetramer at pH 7.6, markedly less ...

13

Regulation of human ribosomal RNA transcription.  

Science.gov (United States)

We have used a cell-free polymerase I transcription system derived from HeLa cells to study the regulation of human rRNA synthesis. Analysis of deletion mutants spanning the start site of transcription at nucleotide +1 indicates that the control region affecting initiation of human rRNA synthesis is contained within sequences from nucleotides -158 to +18. This promoter region can be subdivided into (i) a central segment of approximately 40 base pair that is required for transcription and (ii) flanking sequences that influence the efficiency of transcription in vitro. We have examined the in vitro transcriptional activity of the human extract under various conditions that are thought to modulate rRNA synthesis in vivo. Cell-free extracts prepared from HeLa cells infected with adenovirus 2 synthesize human rRNA at levels greatly decreased relative to uninfected cell extracts. By contrast, in vitro transcription of human rRNA is stimulated 2- to ...

1983-06-01

14

The Arabidopsis ref2 Mutant Is Defective in the Gene Encoding CYP83A1 and Shows Both Phenylpropanoid and Glucosinolate Phenotypes  

UK PubMed Central (United Kingdom)

The Arabidopsis ref2 mutant was identified in a screen for plants having altered fluorescence under UV light. Characterization of the ref2 mutants showed that they...Full Text Available

2003-01-01

15

Nitrogen fixation in nitrate reductase-deficient mutants of cultured rhizobia.  

UK PubMed Central (United Kingdom)

Forty-eight mutants unable to reduce nitrate were isolated from "cowpea" Rhizobium sp. strain 32Hl and examined for nitrogenase activity in culture. All but two of the mutants had nitrogenase activity...Full Text Available

1977-02-01

16

Hormonal Characterization of a Nonrooting Naphthalene-Acetic Acid Tolerant Tobacco Mutant by an Immunoenzymic Method  

UK PubMed Central (United Kingdom)

The comparative analysis of plant hormones was undertaken on a 1-naphthaleneacetic acid tolerant mutant and normal tobacco (Nicotiana tabacum cv Xanthi) plantlets. The mutant plantlet...Full Text Available

1989-01-01

17

Identification of mutations leading to the Lesch-Nyhan syndrome by automated direct DNA sequencing of in vitro amplified cDNA  

Energy Technology Data Exchange (ETDEWEB)

The Lesch-Nyhan (LN) syndrome is a severe X chromosome-linked disease that results from a deficiency of the purine salvage enzyme hypoxanthine phosphoribosyltransferase (HPRT). The mutations leading to the disease are heterogeneous and frequently arise as de novo events. The authors have identified nucleotide alterations in 15 independently arising HPRT-deficiency cases by direct DNA sequencing of in vitro amplified HPRT cDNA. They also demonstrate that the direct DNA sequence analysis can be automated, further simplifying the detection of new mutations at this locus. The mutations include DNA base substitutions, small DNA deletions, a single DNA base insertion, and errors in RNA splicing. The application of these procedures allows DNA diagnosis and carrier identification by the direct detection of the mutant alleles within individual families affected by LN.

1989-03-01

18

Regulation of Glutamate Dehydrogenases in nit-2 and am Mutants of Neurospora crassa  

UK PubMed Central (United Kingdom)

The regulation of the glutamate dehydrogenases was investigated in wild-type Neurospora crassa and two classes of mutants altered in the assimilation of inorganic nitrogen, as either...Full Text Available

1979-03-01

19

Novel strategy for isolating suppressors of meiosis-deficient mutants and its application for isolating the bcy1 suppressor  

KoreaScience (Korea)

Full Text Available

1997-03-01

20

Distribution of Calretinin-immunoreactive Unipolar Brush Cells in Ataxic Mutant Pogo Mice Cerebellum  

KoreaScience (Korea)

Full Text Available

2005-08-01

21

Identification and characterization of noncoding small RNAs in Streptococcus pneumoniae serotype 2 strain D39.  

Science.gov (United States)

We report a search for small RNAs (sRNAs) in the low-GC, gram-positive human pathogen Streptococcus pneumoniae. Based on bioinformatic analyses by Livny et al. (J. Livny, A. Brencic, S. Lory, and M. K. Waldor, Nucleic Acids Res. 34:3484-3493, 2006), we tested 40 candidates by Northern blotting and confirmed the expression of nine new and one previously reported (CcnA) sRNAs in strain D39. CcnA is one of five redundant sRNAs reported by Halfmann et al. (A. Halfmann, M. Kovacs, R. Hakenbeck, and R. Bruckner, Mol. Microbiol. 66:110-126, 2007) that are positively controlled by the CiaR response regulator. We characterized 3 of these 14 sRNAs: Spd-sr17 (144 nucleotides [nt]; decreased in stationary phase), Spd-sr37 (80 nt; strongly expressed in all growth phases), and CcnA (93 nt; induced by competence stimulatory peptide). Spd-sr17 and CcnA likely fold into structures containing single-stranded regions between hairpin structures, whereas Spd-sr37 forms a base-paired structure. Primer ...

2010-01-01

22

ATRX ADD domain links an atypical histone methylation recognition mechanism to human mental-retardation syndrome  

Energy Technology Data Exchange (ETDEWEB)

ATR-X (alpha-thalassemia/mental retardation, X-linked) syndrome is a human congenital disorder that causes severe intellectual disabilities. Mutations in the ATRX gene, which encodes an ATP-dependent chromatin-remodeler, are responsible for the syndrome. Approximately 50% of the missense mutations in affected persons are clustered in a cysteine-rich domain termed ADD (ATRX-DNMT3-DNMT3L, ADD{sub ATRX}), whose function has remained elusive. Here we identify ADD{sub ATRX} as a previously unknown histone H3-binding module, whose binding is promoted by lysine 9 trimethylation (H3K9me3) but inhibited by lysine 4 trimethylation (H3K4me3). The cocrystal structure of ADD{sub ATRX} bound to H3{sub 1-15}K9me3 peptide reveals an atypical composite H3K9me3-binding pocket, which is distinct from the conventional trimethyllysine-binding aromatic cage. Notably, H3K9me3-pocket mutants and ATR-X syndrome mutants are defective in both H3K9me3 ...

2011-07-19

23

Radiation-induced mutations at mouse hemoglobin loci  

International Nuclear Information System (INIS)

In experiments designed to detect new mutations affecting hemoglobin, we irradiated the male or female parent in reciprocal crosses of two mouse strains that differ in alleles at the hemoglobin (Hba, Hbb) loci as well as at five other specific loci. Offspring were analyzed for hemoglobin properties (electrophoretic pattern, solubility, crystal pattern), serum albumin differences, erythrocyte lysis, reticulocyte count, and external appearance. Five hemoglobin variants were found among the mutants. In three, the genetic contribution from the irradiated father was not expressed with regard to the #alpha#-chain; one carried a tandem duplication (the first known case in the mouse) involving Hbb; and one probably resulted from double nondisjunction of chromosome 7. The finding that major chromosome aberratios can mimic hemoglobin mutations indicates the need, in similar experiments, to follow F_1 screening with thorough cytogenetic analysis. The ...

24

Negative regulation of {beta}-catenin/Tcf signaling by naringenin in AGS gastric cancer cell  

Science.gov (United States)

Functional activation of {beta}-catenin/Tcf signaling plays an important role in early events in carcinogenesis. We examined the effect of naringenin against {beta}-catenin/Tcf signaling in gastric cancer cells. Reporter gene assay showed that naringenin inhibited {beta}-catenin/Tcf signaling efficiently. In addition, the inhibition of {beta}-catenin/Tcf signaling by naringenin in HEK293 cells transiently transfected with constitutively mutant {beta}-catenin gene, whose product is not phosphorylated by GSK3{beta}, indicates that its inhibitory mechanism was related to {beta}-catenin itself or downstream components. To investigate the precise inhibitory mechanism, we performed immunofluorescence, Western blot, and EMSA. As a result, our data revealed that the {beta}-catenin distribution and the levels of nuclear {beta}-catenin and Tcf-4 proteins were unchanged after naringenin treatment. Moreover, the binding activities of Tcf complexes to consensus DNA were not ...

2005-09-30

25

Extracts of the marine brown macroalga, Ascophyllum nodosum, induce jasmonic acid dependent systemic resistance in Arabidopsis thaliana against Pseudomonas syringae pv. tomato DC3000 and Sclerotinia sclerotiorum  

British Library Electronic Table of Contents (United Kingdom)

We studied the mechanism of Ascophyllum nodosum (a brown macroalga) induced resistance in Arabidopsis thaliana against Pseudomonas syringae pv. tomato DC3000. Root treatment of A. thaliana Col-0 plants with extracts of A. nodosum [aqueous (ANE), chloroform (C-ANE) and ethylacetate fractions, (E-ANE)] reduced the development of disease symptoms on the leaves. These extracts also induced resistance in salicylic acid deficient NahG and ics1 plants. However, the extracts did not elicit an effect on jar1 (jasmonic acid resistance 1) mutant. A. nodosum extract induced resistance to Pst DC3000 correlated with increased expression of jasmonic acid related gene transcripts PDF1.2 while PR1 and ICS1 expression were less affected. Additionally, pretreatment of Arabidopsis plants with ANE, protected t...

2011-01-01

26

Absence of linkage of apparently single gene mediated ADHD with the human syntenic region of the mouse mutant coloboma  

Energy Technology Data Exchange (ETDEWEB)

Attention deficit disorder (ADHD) is a complex biobehavioral phenotype which affects up to 8% of the general population and often impairs social, academic, and job performance. Its origins are heterogeneous, but a significant genetic component is suggested by family and twin studies. The murine strain, coloboma, displays a spontaneously hyperactive phenotype that is responsive to dextroamphetamine and has been proposed as a genetic model for ADHD. Coloboma is a semi-dominant mutation that is caused by a hemizygous deletion of the SNAP-25 and other genes on mouse chromosome 2q. To test the possibility that the human homolog of the mouse coloboma gene(s) could be responsible for ADHD, we have carried out linkage studies with polymorphic markers in the region syntenic to coloboma (20p11-p12). Five families in which the pattern of inheritance of ADHD appears to be autosomal dominant were studied. Segregation analysis of the traits studied suggested that the best ...

1995-12-18

27

Soybean Mutants Lacking Constitutive Nitrate Reductase Activity 1  

UK PubMed Central (United Kingdom)

The objectives of this study were to select and initially characterize mutants of soybean (Glycine max L. Merr. cv Williams) with decreased ability to reduce nitrate. Selection involved...Full Text Available

1983-06-01

28

Retrograde neuronal tracing with a deletion-mutant rabies virus  

UK PubMed Central (United Kingdom)

We have constructed a deletion-mutant rabies virus encoding EGFP and find it to be an excellent tool for studying detailed morphology and physiology of neurons projecting to injection sites...Full Text Available

2007-01-01

29

Reduced Accumulation of ABA during Water Stress in a Molybdenum Cofactor Mutant of Barley 1  

UK PubMed Central (United Kingdom)

A barley (Hordeum vulgare L.) mutant (Az34) has been identified with low basal levels of abscisic acid (ABA) and with reduced capacity for producing ABA in response to water stress....Full Text Available

1989-06-01

30

Prevention of clinical coliform mastitis in dairy cows by a mutant Escherichia coli vaccine.  

UK PubMed Central (United Kingdom)

A prospective cohort study was undertaken in two commercial California dairies. The treatment group, 246 cows, received three doses of a whole cell bacterin of J5 Escherichia coli (mutant of E. coli...Full Text Available

1989-07-01

31

Photophysics and optical switching in green fluorescent protein mutants  

UK PubMed Central (United Kingdom)

We demonstrate by using low-temperature high-resolution spectroscopy that red-shifted mutants of green fluorescent protein are photo-interconverted among three conformations and are, therefore, not...Full Text Available

2000-03-28

32

O2-dependent methionine auxotrophy in Cu,Zn superoxide dismutase-deficient mutants of Saccharomyces cerevisiae.  

UK PubMed Central (United Kingdom)

Mutant strains of the yeast Saccharomyces cerevisiae which lack functional Cu,Zn superoxide dismutase (SOD-1) do not grow aerobically unless supplemented with methionine. The molecular basis of this...Full Text Available

1990-04-01

33

Molybdoenzyme biosynthesis in Escherichia coli: in vitro activation of purified nitrate reductase from a chlB mutant.  

UK PubMed Central (United Kingdom)

All molybdoenzyme activities are absent in chlB mutants because of their inability to synthesize molybdopterin guanine dinucleotide, which together with molybdate constitutes the molybdenum cofactor...Full Text Available

1992-12-01

34

Involvement of a low-molecular-weight substance in in vitro activation of the molybdoenzyme respiratory nitrate reductase from a chlB mutant of Escherichia coli.  

UK PubMed Central (United Kingdom)

The soluble subcellular fraction of a chlB mutant contains an inactive precursor form of the molybdoenzyme nitrate reductase, which can be activated by the addition to the soluble fraction of protein...Full Text Available

1987-10-01

35

DNA replication in Escherichia coli mutants that lack protein HU.  

UK PubMed Central (United Kingdom)

DNA replication in Escherichia coli cells lacking protein HU was studied. HU has been suggested to be involved in the initiation of replication from in vitro studies. The isolated HU mutants, however,...Full Text Available

1989-10-01

36

Biochemical Characterization of Soybean Mutants Lacking Constitutive NADH:Nitrate Reductase 1  

UK PubMed Central (United Kingdom)

Two nitrate reductase (NR) mutants were selected for low nitrate reductase (LNR) activity by in vivo NR microassays of M2 seedlings derived from nitrosomethylurea-mutagenized...Full Text Available

1986-06-01

37

Analysis of the astray/robo2 zebrafish mutant reveals that degenerating tracts do not provide strong guidance cues for regenerating optic axons  

UK PubMed Central (United Kingdom)

During formation of the optic projection in astray/robo2 mutant zebrafish, optic axons exhibit rostro-caudal pathfinding errors, ectopic midline crossing and...Full Text Available

2010-10-13

38

Aldehyde Oxidase and Xanthine Dehydrogenase in a flacca Tomato Mutant with Deficient Abscisic Acid and Wilty Phenotype1  

UK PubMed Central (United Kingdom)

The flacca tomato (Lycopersicon esculentum) mutant displays a wilty phenotype as a result of abscisic acid (ABA) deficiency. The Mo cofactor (MoCo)-containing aldehyde...Full Text Available

1999-06-01

39

Morphological descriptions of some induced systematic mutants of grapes (Vitis vinifera L.)  

International Nuclear Information System (INIS)

(1972). Germany Sharma, RL Himachal Pradesh Univ., Palampur (India).

40

Missense mutations in the growth hormone receptor dimerization region in Laron syndrome  

Energy Technology Data Exchange (ETDEWEB)

Laron syndrome (LS) is an autosomal recessively inherited condition characterized by insensitivity to endogenous and exogenous GH. Affected individuals have severe episodes and other characteristic features. GH receptor gene mutations are present in all affected individuals in whom molecular studies have been reported. The GH receptor is a plasma membrane-spanning protein in which the extracellular domain binds circulating GH and the intracellular domain interacts with the JAK-2 kinase and possibly other intracellular signaling molecules. GH receptor dimerization occurs on GH binding and is thought to be required for normal signal transduction. We have studied the GH receptor genes of four unrelated individuals affected with LS from the United States, Italy, Saudi Arabia, and India. We have identified four different missense mutations that alter consecutive amino acids 152 to 155 in or near the dimerization domain of the GH ...

1994-09-01

41

Evolution of improved varieties of sesame through induced mutations  

International Nuclear Information System (INIS)

Sesame varieties/genotypes showed a good response to radiation with gamma rays and treatments with EMS. In M_1 both gamma rays and EMS influenced germination, seedling height, survival of plants and pollen fertility/sterility, producing deleterious effects on these characters. Compared to black seeded genotypes, white seeded ones are more susceptible to radiation. Both gamma rays and EMS produced various types of morphological variations in M_2 generation. These were dwarf plant mutants, mottled and fleshy leaved mutants (sterile), fasciated stem, flower colour, altered phyllotaxy, early, uniculm, capsule size variations, multiple capsule/leaf axil, seed coat colour variations, indehiscent and semi-indehiscent type mutants. Moreover, studies on quantitative characters including seed yield revealed the induction of mutants in both positive and negative directions for such traits which made a good scope ...

2001-01-01

42

Uncoupler-resistant mutants of bacteria.  

UK PubMed Central (United Kingdom)

The chemiosmotic model of energy transduction offers a satisfying and widely confirmed understanding of the action of uncouplers on such processes as oxidative phosphorylation; the uncoupler, by facilitating...Full Text Available

1990-03-01

43

The vacuolar processing enzyme (VPE) mutation suppresses an HR-like cell death induced by the double knockout mutant of vacuolar Ca2+-ATPases in Arabidopsis  

KoreaScience (Korea)

Full Text Available

2011-06-01

44

Paraquat toxicity is increased in Escherichia coli defective in the synthesis of polyamines  

International Nuclear Information System (INIS)

The authors have shown that toxicity of paraquat for Escherichia coli is increased over 1-fold in strains defective in the biosynthesis of spermidine compared to isogenic strains containing spermidine. The increased sensitivity of these spermidine-deficient mutants to paraquat is eliminated by growth in medium containing spermidine or by endogenous supplementation of spermidine by the use of a speE"+D"+ plasmid. No paraquat toxicity is seen in the absence of oxygen, even in amine-deficient strains, indicating that superoxide is the agent responsible for the increased toxicity. However, the specific mechanisms responsible for the increased paraquat toxicity in the spermidine-deficient mutants remain to be determined. The marked sensitivity to paraquat of E. coli deficient in spermidine is of particular interest, since such mutants have no other phenotypic properties that can be easily assayed. This increased sensitivity has ...

45

Hormonal Control of Cell Proliferation Requires PASTICCINO Genes  

UK PubMed Central (United Kingdom)

PASTICCINO (PAS) genes are required for coordinated cell division and differentiation during plant development. In loss-of-function pas mutants,...Full Text Available

2003-07-01

46

Effect of salt stress and phosphorus deficiency in mutants of rhizobium obtained by gamma irradiation  

International Nuclear Information System (INIS)

Two strains of Rhizobium: Rhizobium Tropici and Mesorhizobium Ciceri nodulating respectively common bean and chickpea were treated by gamma irradiation (60Co) source. Radiosensibility analysis showed that 800 Gy was the biggest dose supported by these two strains. We isolated gamma irradiated resistant strain in order to select mutant of them which can supported salt stress and phosphorus deficiency. Salinity analysis showed that Mesorhizobium Ciceri 835 strain, can tolerate up to 18g/l (273 mM NaCl) of salt, whereas, their irradiation mutants tolerate salinity up to 33g/l (564mM. NaCl) Rhizobium Tropici CIAT899 can survive at 20g/l (342 mM) either for control strain or mutants. Analysis of phosphorus deficiency showed that either Rhizobium Tropici CIAT899, or Mesorhizobium Ciceri 835 can survive in medium without phosphore. Our results permit us to screen mutants tolerant to these stresses wide spread ...

2008-08-12

47

Defective gut function in drop-dead mutant Drosophila  

UK PubMed Central (United Kingdom)

Mutation of the gene drop-dead (drd) causes adult Drosophila to die within 2 weeks of eclosion and is associated with reduced rates of defecation...Full Text Available

2009-09-01

48

Defective Glycinergic Synaptic Transmission in Zebrafish Motility Mutants  

UK PubMed Central (United Kingdom)

Glycine is a major inhibitory neurotransmitter in the spinal cord and brainstem. Recently, in vivo analysis of glycinergic synaptic transmission has been pursued in zebrafish using...Full Text Available

49

Reactive biomolecular divergence in genetically altered yeast cells and isolated mitochondria as measured by biocavity laser spectroscopy : a rapid diagnostic method for studying cellular responses to stress and disease.  

Energy Technology Data Exchange (ETDEWEB)

We report an analysis of four strains of baker's yeast (Saccharomyces cerevisiae) using biocavity laser spectroscopy. The four strains are grouped in two pairs (wild type and altered), in which one strain differs genetically at a single locus, affecting mitochondrial function. In one pair, the wild-type rho+ and a rho0 strain differ by complete removal of mitochondrial DNA (mtDNA). In the second pair, the wild-type rho+ and a rho- strain differ by knock-out of the nuclear gene encoding Cox4, an essential subunit of cytochrome c oxidase. The biocavity laser is used to measure the biophysical optic parameter Deltalambda, a laser wavelength shift relating to the optical density of cell or mitochondria that uniquely reflects its size and biomolecular composition. As such, Deltalambda is a powerful parameter that rapidly interrogates the biomolecular state of single cells and mitochondria. Wild-type cells and mitochondria produce Gaussian-like distributions ...

2006-12-01

50

Two proteins encoded at the chlA locus constitute the converting factor of Escherichia coli chlA1.  

UK PubMed Central (United Kingdom)

Molybdopterin (MPT) is not produced by the Escherichia coli mutants chlA1, chlM, or chlN or by the Neurospora crassa mutant nit-1. Extracts of E. coli chlA1 contain an activity, the converting factor,...Full Text Available

1989-06-01

51

Relationship of large and small invertases in Saccharomyces: mutant selectively deficient in small invertase.  

UK PubMed Central (United Kingdom)

A mutant strain of Saccharomyces cerevisiae (D10-ER1) has been isolated after a two-step mutagenesis of strain 4059-358D (SUC 1) using ethyl methane sulfonate. Cells of this new strain produced a level...Full Text Available

1978-09-01

52

RecA protein acts at the initiation of stable DNA replication in rnh mutants of Escherichia coli K-12.  

UK PubMed Central (United Kingdom)

Escherichia coli rnh mutants lacking RNase H activity are capable of recA+-dependent DNA replication in the absence of concomitant protein synthesis (stable DNA replication). In rnh dnaA::Tn10 and rnh...Full Text Available

1985-08-01

53

Isolation and Initial Characterization of Constitutive Nitrate Reductase-Deficient Mutants NR328 and NR345 of Soybean (Glycine max) 1  

UK PubMed Central (United Kingdom)

Two nitrate reductase deficient mutants of soybean (Glycine max [L.] Merr. cv Bragg) were isolated from approximately 10,000 M2 seedlings, using a direct enzymic assay in...Full Text Available

1986-06-01

54

Impaired neonatal survival of pro-opiomelanocortin null mutants.  

Science.gov (United States)

Intercrosses of heterozygous pro-opiomelanocortin (POMC) mice result in homozygous null progeny at lower frequencies than expected. Genotyping offspring at pre-, peri-, and postnatal stages revealed that over half of homozygous null mutants die in the early postnatal stages. To investigate the reasons for this early postnatal lethality, we analyzed in detail different parameters in the initial hours after birth. POMC null mutants born to heterozygous dams presented at birth with corticosterone levels no different from wildtype littermates, were euglycemic, and had normal liver glycogen stores. However, already 30 min after birth corticosterone levels dropped by 80% and were undetectable thereafter, while corticosterone levels in wildtype animals increased during postnatal hours. Circulating adrenaline was almost below detection 1h after birth. Blood glucose levels fell sharply in all genotypes within 30 min after birth; however, wildtype and ...

2010-12-22

55

Comparison between NOx Evolution Mechanisms of Wild-Type and nr1 Mutant Soybean Leaves 1  

UK PubMed Central (United Kingdom)

The nr1 soybean (Glycine max [L.] Merr.) mutant does not contain the two constitutive nitrate reductases, one of which is responsible for enzymic conversion of nitrite to...Full Text Available

1990-05-01

56

Assembly of 60S ribosomal subunits is perturbed in temperature-sensitive yeast mutants defective in ribosomal protein L16.  

UK PubMed Central (United Kingdom)

Temperature-sensitive mutants defective in 60S ribosomal subunit protein L16 of Saccharomyces cerevisiae were isolated through hydroxylamine mutagenesis of the RPL16B gene and plasmid shuffling. Two...Full Text Available

1991-11-01

57

The reaction of hydroxylamine with bacteriorhodopsin studied with mutants that have altered photocycles: Selective reactivity of different photointermediates  

Energy Technology Data Exchange (ETDEWEB)

The reaction of the retinylidene Schiff base in bacteriorhodopsin (bR) to the water-soluble reagent hydroxylamine is enhanced by greater than 2 orders of magnitude under illumination. We have used this reaction as a probe for changes in Schiff base reactivity during the photocycle of wild-type bR and mutants defective in proton transport. We report here that under illumination at pH 6, the D85N mutant has a 20-fold lower rate and the D212N mutant has a greater than 4-fold higher rate for the light-dependent reaction with hydroxylamine compared with wild-type bR. In contrast, the reactivities of wild-type bR and the D96N and T46V mutants are similar. It has been previously shown that the D96N and T46V replacements have no significant effect on the kinetics of M formation but have dramatic effects on rate of the decay of M. We therefore conclude that the hydroxylamine reaction occurs before formation of ...

1991-03-15

58

Evaluation of induced peanut mutant (RT-1010) for Pod yield, oil yield and resistant to aspergillus spp. invation and aflatoxin contamination  

International Nuclear Information System (INIS)

An induced mutant, RT-10 (M6 generation) was re-irradiated with 300 Gy of gamma rays. In M2 generation a mutant plant was identified, which characterized with large pods, prominent longitudinal ribbon reticulation and slight constriction. The selected mutant, RT-1010 has the highest pod yield (.3.59 t/fed.vs.2.91 and 2.61 t/fed respectively) and harvest index ( 0.52 vs. 0.42 and 0.45 resp.), the best in fancy pods percentage (57.67 % vs. 38.33 % and 44.17 % resp.), TSMK grade (98.96 % vs. 83.03 % and 84.05 % resp.), the largest pod mass (253.37 g vs . 211.32 g and 222.19 g resp.) and the highest expected oil yield (1370 Kg/fed vs. 895.44 Kg and 868.17 Kg/fed. resp.). With regard to fatty acid compositions, both the selected mutant, RT-1010 and the parental mutant RT-10 have almost a similar high oleic fatty acid (C18:1) percentage (56.35 % and 55.103 % respectively), low linoleic ...

2010-01-01

59

Involvement of a putative response regulator Brrg-1 in the regulation of sporulation, sensitivity to fungicides, and osmotic stress in Botrytis cinerea  

British Library Electronic Table of Contents (United Kingdom)

The response regulator protein is a core element of two-component signaling pathway. In this study, we investigated functions of BRRG-1 of Botrytis cinerea, a gene that encodes a putative response regulator protein, which is homologous to Rrg-1 in Neurospora crassa. The BRRG-1 gene deletion mutant ?Brrg1-62 was unable to produce conidia. The mutant showed increased sensitivity to osmotic stress mediated by NaCl and KCl, and to oxidative stress generated by H2O2. Additionally, the mutant was more sensitive to the fungicides iprodione, fludioxonil, and triadimefon than the parental strain. Western-blot analysis showed that the Bos-2 protein, the putative downstream component of Brrg-1, was not phosphorylated in the ?Brrg1-62. Real-time polymerase chain reaction assays showed that expression ...

2011-01-01

60

The first long-lived mutants: discovery of the insulin/IGF-1 pathway for ageing  

UK PubMed Central (United Kingdom)

Inhibiting insulin/IGF-1 signalling extends lifespan and delays age-related disease in species throughout the animal kingdom. This life-extension pathway, the first to be defined, was discovered through...Full Text Available

2011-01-12

61

The adenovirus-2 EIIa early gene promoter: sequences required for efficient in vitro and in vivo transcription.  

UK PubMed Central (United Kingdom)

A series of deletion mutants extending from -250 toward the capsite has been constructed in the early promoter region of the adenovirus 2 EIIa gene and tested both in vitro, and in vivo after transfection...Full Text Available

1983-10-25

62

Purification and properties of penicillinases from two strains of Bacillus licheniformis: a chemical, physicochemical and physiological comparison  

UK PubMed Central (United Kingdom)

1. The penicillinases formed by penicillinase-constitutive mutant strains from two closely related varieties (749 and 6346) of Bacillus licheniformis have been isolated, characterized...Full Text Available

1965-03-01

63

Oxidation of an engineered pore cysteine locks a voltage-gated K+ channel in a nonconducting state.  

UK PubMed Central (United Kingdom)

We report the use of cysteine-substituted mutants in conjunction with in situ oxidation to determine the physical proximity of a pair of engineered cysteines in the pore region of the voltage-gated...Full Text Available

1996-12-01

64

Mutations in cyr1 and pat1 reveal pheromone-induced G1 arrest in the fission yeast Schizosaccharomyces pombe  

DEFF Research Database (Denmark)

Investigations into sexual differentiation and pheromone response in the fission yeast Schizosaccharomyces pombe are complicated by the need to first starve the cells of nitrogen. Most mating-related experiments are therefore performed on non-dividing cells. Here we overcome this problem by using two mutants that bypass the nutritional requirements and respond to the M-factor mating pheromone in rich medium. The first mutant lacks the cyr1 gene which encodes adenylate cyclase and these cells contain no measurable amounts of cAMP. When M-factor is added to a growing h+ cyr1- strain it causes a transient G1 arrest of cell division, transcription of mat1-Pm, and elongation of the cells to form shmoos. The second mutant contains the temperature-sensitive pat1-114 allele. At 30 degrees C this mutant was previously shown not only to bypass the nutritional signal but also to stop growing in a state derepressed ...

1994-01-01

65

Mutant ?-Synuclein Overexpression Mediates Early Proinflammatory Activity  

UK PubMed Central (United Kingdom)

Microglia provide immune surveillance for the brain through both the removal of cellular debris and protection against infection by microorganisms and “foreign” molecules. Upon...Full Text Available

2009-10-01

66

Modulation of lipid biosynthesis contributes to stress resistance and longevity of C. elegans mutants  

UK PubMed Central (United Kingdom)

Many lifespan-modulating genes are involved in either generation of oxidative substrates and end-products, or their detoxification and removal. Among such metabolites, only lipoperoxides have the ability...Full Text Available

67

Knockout of Arabidopsis ACCELERATED-CELL-DEATH11 encoding a sphingosine transfer protein causes activation of programmed cell death and defense  

UK PubMed Central (United Kingdom)

We describe the lethal, recessive accelerated-cell-death11 Arabidopsis mutant (acd11). Cell death in acd11 exhibits characteristics of animal apoptosis...Full Text Available

2002-02-15

68

Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin  

UK PubMed Central (United Kingdom)

BackgroundChondroitin sulphate is a complex polysaccharide having important structural and protective functions in animal tissues. Extracted from animals, this compound is used as...Full Text Available

69

Investigation and prediction of the severity of p53 mutants using parameters from structural calculations  

UK PubMed Central (United Kingdom)

A method has been developed to predict the effects of mutations in the p53 cancer suppressor gene. The new method uses novel parameters combined with previously established parameters. The most important...Full Text Available

2009-08-01

70

Identification of the Haemophilus influenzae tolC Gene by Susceptibility Profiles of Insertionally Inactivated Efflux Pump Mutants  

UK PubMed Central (United Kingdom)

Isogenic strains containing insertional disruptions of 10 Haemophilus influenzae Rd genes were investigated for their effects on the susceptibility of the organism to various classes...Full Text Available

2004-04-01

71

Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels  

UK PubMed Central (United Kingdom)

Calorie restriction (CR) induces a metabolic shift towards mitochondrial respiration; however, molecular mechanisms underlying CR remain unclear. Recent studies suggest that CR-induced mitochondrial...Full Text Available

72

Identification and Disruption of Two Discrete Loci Encoding Hyaluronic Acid Capsule Biosynthesis Genes hasA, hasB, and hasC in Streptococcus uberis  

UK PubMed Central (United Kingdom)

The hyaluronic acid capsule of Streptococcus uberis has been implicated in conferring resistance to phagocytosis by bovine neutrophils. Construction of a bank of random insertion mutants...Full Text Available

2001-01-01

73

High-Throughput Screen for Poly-3-Hydroxybutyrate in Escherichia coli and Synechocystis sp. Strain PCC6803  

UK PubMed Central (United Kingdom)

A novel, quantitative method for detecting poly-3-hydroxybutyrate (PHB) amounts in viable cells was developed to allow for high-throughput screening of mutant libraries. The staining technique was demonstrated...Full Text Available

2006-05-01

74

Gene-enzyme telationships in somatic cells and their organismal derivatives in higher plants. Progress report  

Energy Technology Data Exchange (ETDEWEB)

Progress is reported in the following subject areas: (1) chemistry of the arogenate molecule; (2) plant enzymology at the organismal level; (3) isolation of regulatory mutants in tobacco; and (4) stability of the haploid state in Nicotiana sylvestris.

1980-04-21

75

Gene expression profiling of oxidative stress response of C. elegans aging defective AMPK mutants using massively parallel transcriptome sequencing  

UK PubMed Central (United Kingdom)

BackgroundA strong association between stress resistance and longevity in multicellular organisms has been established as many mutations that extend lifespan also show increased...Full Text Available

76

Developmental abnormalities and epimutations associated with DNA hypomethylation mutations.  

UK PubMed Central (United Kingdom)

A number of aberrant morphological phenotypes were noted during propagation of the Arabidopsis thaliana DNA hypomethylation mutant, ddm1, by repeated self-pollination. Onset of a spectrum of morphological...Full Text Available

1996-10-29

77

Defects in the Secretory Pathway and High Ca2+ Induce Multiple P-bodies  

UK PubMed Central (United Kingdom)

mRNA is sequestered and turned over in cytoplasmic processing bodies (PBs), which are induced by various cellular stresses. Unexpectedly, in Saccharomyces cerevisiae, mutants of the...Full Text Available

2010-08-01

78

Cytokinin-Deficient Transgenic Arabidopsis Plants Show Multiple Developmental Alterations Indicating Opposite Functions of Cytokinins in the Regulation of Shoot and Root Meristem Activity  

UK PubMed Central (United Kingdom)

Cytokinins are hormones that regulate cell division and development. As a result of a lack of specific mutants and biochemical tools, it has not been possible to study the consequences of cytokinin...Full Text Available

2003-11-01

79

Construction and Characterization of Genetically Defined aro omp Mutants of Enterotoxigenic Escherichia coli and Preliminary Studies of Safety and Immunogenicity in Humans  

UK PubMed Central (United Kingdom)

Enterotoxigenic Escherichia coli (ETEC) is a leading cause of diarrhea in travelers to countries where the disease is endemic and causes a major disease burden in the indigenous population,...Full Text Available

2001-08-01

80

Construction and Application of a Zebrafish Array CGH Platform  

UK PubMed Central (United Kingdom)

The zebrafish is emerging as a prominent model system for studying the genetics of human development and disease. Genetic alterations that underlie each mutant model can exist in the form of...Full Text Available

2009-02-01

81

Comparative transcripts profiling reveals new insight into molecular processes regulating lycopene accumulation in a sweet orange (Citrus sinensis) red-flesh mutant  

UK PubMed Central (United Kingdom)

BackgroundInterest in lycopene metabolism and regulation is growing rapidly because accumulative studies have suggested an important role for lycopene in human health promotion....Full Text Available

82

Characterization of T-Even Bacteriophage Substructures  

UK PubMed Central (United Kingdom)

Tail plates obtained from T4D amber mutants were examined with respect to sedimentation behavior, subunit molecular weights, amino acid composition, isoelectric points, and morphology. Intact plates...Full Text Available

1970-10-01

83

Biochemical analysis of PIFTC3, the Trypanosoma brucei ortholog of nematode DYF-13, reveals interactions with established and putative intraflagellar transport components  

UK PubMed Central (United Kingdom)

DYF-13, originally identified in C. elegans within a collection of dye-filling chemosensory mutants, is one of several proteins that have been classified as putatively involved...Full Text Available

2010-10-01

84

Behavioral consequences of dopamine deficiency in the Drosophila central nervous system  

UK PubMed Central (United Kingdom)

The neuromodulatory function of dopamine (DA) is an inherent feature of nervous systems of all animals. To learn more about the function of neural DA in Drosophila, we generated mutant...Full Text Available

2011-01-11

85

An Arabidopsis Mutant Resistant to Thaxtomin A, a Cellulose Synthesis Inhibitor from Streptomyces SpeciesW?  

UK PubMed Central (United Kingdom)

Thaxtomin A is a phytotoxin produced by Streptomyces scabies and other Streptomyces species, the causative agents of common scab disease in potato and other taproot...Full Text Available

2003-08-01

86

A new gene (alkB) of Escherichia coli that controls sensitivity to methyl methane sulfonate.  

UK PubMed Central (United Kingdom)

Seven mutants of Escherichia coli were isolated that are sensitive to methyl methane sulfonate but not to UV light. They exhibited decreased host cell reactivation capacity for methyl methane sulfonate-treated...Full Text Available

1983-03-01

87

A Turquoise Mutant Genetically Separates Expression of Genes Encoding Phycoerythrin and Its Associated Linker Peptides  

UK PubMed Central (United Kingdom)

During complementary chromatic adaptation (CCA), cyanobacterial light harvesting structures called phycobilisomes are restructured in response to ambient light quality shifts. Transcription of genes...Full Text Available

2002-02-01

88

Effect of ionizing radiation and chemical mutagens on vegetatively reproducing plants. Deistvie ioniziruyushchikh izluchenii i khimicheskikh mutagenov na vegetativno razmnozhaemye rasteniya  

Energy Technology Data Exchange (ETDEWEB)

A study is made of plant sensitivity to mutagens during the processing of seeds. An examination is made of problems concerned with modification mutability, the nature of plant restoration following irradiation. Particular attention is given to mutation changes. A presentation is made of several features of mutant changes initially selected by separate indicators. An examination is made of the possible study of mutant indicator genetics. General data on the forms and varieties produced by the mutagenesis method are given. The book is designed for breeding specialists and geneticists engaged in the study of mutagenesis. 439 references, 27 figures, 66 table.

1981-01-01

89

Antifungal activity of Glycyrrhiza glabra extracts and its active constituent glabridin  

British Library Electronic Table of Contents (United Kingdom)

Glabridin, an active constituent of Glycyrrhiza glabra roots, was found to be active against both yeast and filamentous fungi. Glabridin also showed resistance modifying activity against drug resistant mutants of Candida albicans at a minimum inhibitory concentration of 31.25-250 g/mL. Although the compound was reported earlier to be active against Candida albicans, but this is the first report of its activity against drug resistant mutants. Copyright Copyright 2009 John Wiley & Sons, Ltd.

2009-01-01

90

Global Molecular Characterization of the Chromate Stress Response in Shewanella oneidensis MR-1: Identification of a Putative DNA-Binding Response Regulator and Azoreductase Involved in Cr(VI) Detoxification  

Energy Technology Data Exchange (ETDEWEB)

Shewanella oneidensis MR-1 is a model environmental organism that possesses diverse respiratory capacities, including the ability to reduce soluble Cr(VI) to sparingly soluble, less toxic Cr(III). Effective bioremediation of Cr-contaminated sites requires knowledge of the molecular mechanisms and regulation of heavy metal resistance and biotransformation by dissimilatory metal-reducing bacteria. Towards this goal, our ERSP-funded work is focused on the identification and functional analysis of genes/proteins comprising the response pathways for chromate detoxification and/or reduction. Previous transcriptomic profiling and whole-cell proteomic analyses implicated the involvement of a functionally undefined DNA-binding response regulator (SO2426) and a putative azoreductase (SO3585) in the chromate stress response of MR-1. Here we describe a detailed functional analysis of SO2426 and SO3585 in order to begin to understand the role of these proteins in the cellular response to chromate. ...

2006-04-05

91

biotechnological studies on the effect of gamma rays on peanut  

International Nuclear Information System (INIS)

local peanut cultivars Giza 4 and Giza 5 were exposed to different doses ( 50,100,150,200,250 and 300 Gy)to Co "6"0 gamma ray source . all irradiated materials were cultivated to give the M_1 and M_2 generation , then high yielding mutants were selected from the M_2 generation and cultivated separately to give the M_3 generation. some yield component traits were measured for the three generations (M_1,M_2 and M_3) and M_3 families.variations observed in these traits were attributed to genetic factors. the M_3 selected mutants were artificially infected by aspergillus flavus L spores (aflatoxins-B1 and B2 groups-producer strain). aflatoxin concentrations were estimated and the analysis showed that aflatoxin concentrations in seeds of the M_3 selected mutants from cultivar Giza 4 were higher than those in seeds of the M3 mutants selected from cultivar Giza 5. however, the concentration of aflatoxin ...

2007-01-01

92

Radiation effects on shoot tip culture of chrysanthemum, 2  

International Nuclear Information System (INIS)

(1) Young chrysanthemums were planted after being irradiated at the time of shoot tip culture and the influence of irradiation was evaluated from the point of view of establishment of mutants. (2) Two cultivars, Kin-tenryu and Tenryu-no-asa were used in this experiment. The percentage of cultivars healthy enough to transplant on June 10, 1972 showed a decrease in proportion to the increase of dosage. Some plants of cultivar Kin-tenryu died after being transplanted. (3) Few plants of either cultivar could overwinter, and in particular no plant irradiated by gamma-rays of 20 kR. (4) Plant height showed a tendency of inhibition in both cultivars. (5) Three chlorophyll mutants showing chimera appeared. One of them was in the cultivar Tenryu-no-asa irradiated by 10 kR at 3 days after planting; two of them were in the cultivar Tenryu-no-asa irradiated by 10 kR at 5 days after planting. One mutant exhibiting malformation of entire ...

93

In vitro and in vivo activities of T4 endonuclease V mutants altered in the C-terminal aromatic region  

International Nuclear Information System (INIS)

Genes encoding mutants of the thymine photodimer repair enzyme from bacteriophage T4 (T4 endonuclease V) having an amino acid substitution (T127M, W128A, W128S, Y129A, K130L, Y131A, Y132A) were constructed by use of a previously obtained synthetic gene and expressed in Escherichia coli under the control of the E. coli tryptophan promoter. An in vitro assay of partially fractionated mutant proteins for glycosylase activity was performed with chemically synthesized substrates containing a thymine photodimer. T127M and K130L showed almost the same activity as the wild-type protein. Although W128S, Y131A, and Y132A were slightly active, W128A and Y129A lost activity. The results indicated that the aromatic amino acids around position 130 may be important for the glycosylase activity. Mutant T127M was purified, and the Km value was found to be of the same order as that of the wild type (10(-8) M). In vivo activities for all ...

94

Detection of basepair substitution mutation at a frequency of 1 x 10(-7) by combining two genotypic selection methods, MutEx enrichment and allele-specific competitive blocker PCR.  

Science.gov (United States)

The detection of rare mutations has many important applications, including risk assessment of drugs and chemicals, measuring environmental exposures to genotoxins, and cancer cell detection. A sensitive genotypic selection method has been developed that combines two different mutant allele selection techniques, MutEx enrichment and allele-specific competitive blocker PCR (ACB-PCR). This method was developed and evaluated for the detection of a CAA --> AAA mutation at codon 61 of the mouse H-ras gene. The MutEx enrichment is based on MutS binding to a mismatched basepair in heteroduplex DNA. The bound MutS protects the mutant allele from degradation during subsequent exonuclease treatment. ACB-PCR preferentially amplifies a mutant allele in a PCR reaction using a primer that has more mismatches to the wild-type allele than the mutant allele. By combining these two approaches, the codon 61 mutation was ...

1998-01-01

95

Replacement of leucine-93 by alanine or threonine slows down the decay of the N and O intermediates in the photocycle of bacteriorhodopsin: Implications for proton uptake and 13-cis-retinal----all-trans-retinal reisomerization  

Energy Technology Data Exchange (ETDEWEB)

The authors report that the replacement of Leu-93 in bacteriorhodopsin by Ala (L93A) or Thr (L93T) slows down the photocycle by approximately 100-fold relative to wild-type bacteriorhodopsin. Time-resolved visible absorption spectroscopy and resonance Raman experiments, respectively, show the presence of long-lived O-like and N-like intermediates in the photocycles of the above mutants. We infer the existence of an equilibrium between the N and O intermediates in the photocycles of these mutants. The L93A and L93T mutants exhibit normal proton pumping under continuous illumination, suggesting that the decay of the N and/or O intermediate, and consequently, proton translocation, can be accelerated by the absorption of a second photon. Since the 13-cis----all-trans reisomerization of retinal is completed during the decay of the N and O intermediates, they conclude that the interaction of Leu-93 with retinal is important in ...

1991-08-01

96

Mitochondrial genetic damage induced in yeast by a photoactivated furocoumarin in combination with ethidium bromide or ultraviolet light  

International Nuclear Information System (INIS)

Ethidium bromide (EB) and ultraviolet light (UV) in combination are known to produce a synergistic induction of 'petite' mutants in yeast. Two other agents were combined with EB, 3-Carbethoxypsoralene (3 CPs) activated by 365 nm light or #gamma# rays. EB in combination with 3 CPs also resulted in an enhanced production of 'petite' mutants. After the photoaddition of 3 CPs in exponential phase cells, recovery of the 'petite' mutation during dark liquid holding was inhibited by the presence of EB producing an enhanced number of 'petite' mutants. The behavior of mitochondrial antibiotic resistance markers after individual and combined treatments with EB and 3 CPs indicates a random loss of markers after EB and a preferential loss of a certain region for the 3 CPs photoaddition. The combination of the two agents leads to an additivity of total drug marker losses rather than a synergistic loss. The combination of EB with #gamma# ...

97

Characterization of a novel missense mutation on murine Pax3 through ENU mutagenesis.  

Science.gov (United States)

N-ethyl-N-nitrosourea (ENU) mutagenesis has led to the elucidation of several regulator genes for melanocyte and skin development. Here we characterized a mutant from ENU mutagenesis with similar phenotype as that of Splotch mutant, including exencephaly, spina bifida and abnormal limbs in homozygotes as well as white belly spotting and occasionally loop-tail in heterozygotes. This novel mutant was named as Sp(xG). Through genome-wide linkage analysis in backcross progenies with microsatellite markers, the Sp(xG) was confined to a region between D1MIT415 and D1MIT7 on chromosome 1, where notable Pax3 gene was located. Direct sequencing revealed that Sp(xG) carried a nucleotide A894G missense transition in exon 6 of Pax3 gene that resulted in Asn to Asp substitution at amino acid 269 within the highly-conserved homeodomain (HD) DNA recognition module, which was the first point mutation found in this domain in mice. This ...

2011-07-19

98

6-Methyl-1,2,4-benzenetriol, a new intermediate in penicillic acid biosynthesis in Penicillium cyclopium  

International Nuclear Information System (INIS)

Penicillic acid-negative mutants were obtained from a color mutant derived from Penicillium cyclopium NRRL 1888 through N-methyl-N'-nitro-N-nitrosoguanidine treatment. One mutant (SK2N6) accumulated 6-methyl-1,2,4-benzenetriol, which was not previously known to be a metabolite of P. cyclopium, in addition to orsellinic acid and orcinol. The radioactivity of [1-"1"4C]acetic acid was rapidly incorporated into 6-methyl-1,2,4-benzenetriol in a culture of P. cyclopium SK2N6. Moreover, the radioactivity of ["1"4C]6-methyl-1,2,4-benzenetriol was efficiently incorporated into penicillic acid in a culture of P. cyclopium NRRL 1888. These data indicate that 6-methyl-1,2,4-benzenetriol is a precursor for penicillic acid biosynthesis. The results on the addition of 1,4-dihydroxy-6-methoxy-2-methylbenzene, 6-methoxy-2-methylbenzoquinone (1,4), and 1-O-methylorcinol to a culture of P. cyclopium SK2N6 indicated that only the former two ...

99

Comparative characteristics of radiation accidents affecting population  

International Nuclear Information System (INIS)

Short communication.

1994-10-25

100

The DFNA5 gene, responsible for hearing loss and involved in cancer, encodes a novel apoptosis-inducing protein  

British Library Electronic Table of Contents (United Kingdom)

DFNA5 was first identified as a gene causing autosomal dominant hearing loss (HL). Different mutations have been found, all exerting a highly specific gain-of-function effect, in which skipping of exon 8 causes the HL. Later reports revealed the involvement of the gene in different types of cancer. Epigenetic silencing of DFNA5 in a large percentage of gastric, colorectal and breast tumors and p53-dependent transcriptional activity have been reported, concluding that DFNA5 acts as a tumor suppressor gene in different frequent types of cancer. Despite these data, the molecular function of DFNA5 has not been investigated properly. Previous transfection studies with mutant DFNA5 in yeast and in mammalian cells showed a toxic effect of the mutant protein, which was not seen after transfection ...

2011-01-01

101

Somatic mutations detected by immunofluorescence and flow cytometry  

International Nuclear Information System (INIS)

We have at hand the tools for development of both a single amino acid substitution and a gene loss type assay for somatic cell mutations in human red cells. We are close to being able to assess the assays as possible means for screening the human population for individuals who may be a risk of acquiring abnormally high frequencies of mutant cells. The cause for such a high frequency of mutant cells could be a prior exposure of the individual to large amount of mutagen, or it could indicate a member of a vulnerable subpopulation that may have poor DNA repair systems. Either of these cases could well be an indication that the particular individual is at high risk that exposure to mutagens could lead to potential pathology. In the near future we expect to determine the reliability of the assays and to perform experiments to confirm the validity of flow cytometry in measuring real somatic mutagenic events that occur in vivo. (orig./AJ).

102

[Intraspecies antagonism of Sh. flexneri in an HEp-2 cell line model].  

Science.gov (United States)

The authors describe an effect of suppression of invasion of the guinea pig eye conjunctiva and the HEp-2 epithelial cells by virulent Sh. flexneri bacilli, with a simultaneous administration of the same dose of avirulent shigella mutants, genetically connected with them. The data of morphological study and experiments with 3H-glucose labeled shigellae carried out on the cell species model indicated that the bacterial competition for the specific sites for absorption on the epithelial cells underlay the observed phenomenon. PMID:331774

1977-06-01

103

Yeast allosteric chorismate mutase is locked in the activated state by a single amino acid substitution  

International Nuclear Information System (INIS)

Chorismate mutase, a branch-point enzyme in the aromatic amino acid pathway of Saccharomyces cerevisiae, and also a mutant chorismate mutase with a single amino acid substitution in the C-terminal part of the protein have been purified approximately 20-fold and 64-fold from overproducing strains, respectively. The wild-type enzyme is activated by tryptophan and subject to feedback inhibition by tyrosine, whereas the mutant enzyme does not respond to activation by tryptophan nor inhibition by tyrosine. Both enzymes are dimers consisting of two identical subunits of M_r 30,000, each one capable of binding one substrate and one activator molecule. Each subunit of the wild-type enzyme also binds one inhibitor molecule, whereas the mutant enzyme lost this ability. The enzyme reaction was observed by "1H NMR and shows a direct and irreversible conversion of chorismate to prephenate without the accumulation of any enzyme-free ...

104

Viral suppression of multiple escape mutants by de novo CD8+ T cell responses in a human immunodeficiency virus-1 Infected elite suppressor  

UK PubMed Central (United Kingdom)

Elite suppressors or controllers (ES) are HIV-1 infected patients who maintain undetectable viral loads without treatment. While HLA-B*57-positive ES are usually infected with virus that is unmutated...Full Text Available

105

Unraveling photosystems. Final technical report  

Energy Technology Data Exchange (ETDEWEB)

This report highlights four main points. (1) A residue substitution in phosphoribulokinase of Synechocystis PCC 6803 renders the mutant light-sensitive. The authors isolated a light-sensitive mutant (BRLS) of the photosynthetic cyanobacterium Synechocystis 6803 that does not survive exposure to bright light; 70% of BRLS cells die upon exposure to light of > 3000 lux for 2 hr. (2) Excitation energy transfer from phycocyanin to chlorophyll in an apcA-defective mutant of Synechocystis sp. PCC 6803. A greenish mutant of the normally bule-green cyanobacterium Synechocystis sp. PC 6803, designated UV6p, was isolated and characterized. UV6p possesses functional photosystems I and II but lacks normal light harvesting phycobilisomes because allophycocyanin is absent and core-specific linker proteins are almost entirely absent. (3) Deletion of the psbG1 gene of the cyanobacterium Synechocystis sp. PCC 6803 ...

1997-09-01

106

Quantitative transfer of the molybdenum cofactor from xanthine oxidase and from sulphite oxidase to the deficient enzyme of the nit-1 mutant of Neurospora crassa to yield active nitrate reductase.  

UK PubMed Central (United Kingdom)

An assay method is described for measurement of absolute concentrations of the molybdenum cofactor, based on complementation of the defective nitrate reductase ('apo nitrate reductase') in extracts...Full Text Available

1984-04-15

107

Phosphorylation of ?6-Tubulin by Protein Kinase C? Activates Motility of Human Breast Cells*  

UK PubMed Central (United Kingdom)

Engineered overexpression of protein kinase Cα (PKCα) was previously shown to endow nonmotile MCF-10A human breast cells with aggressive motility. A traceable mutant of PKCα...Full Text Available

2009-06-26

108

Mutational Analysis of the Carboxy-Terminal (YGX)4 Repeat Domain of CpsD, an Autophosphorylating Tyrosine Kinase Required for Capsule Biosynthesis in Streptococcus pneumoniae  

UK PubMed Central (United Kingdom)

In Streptococcus pneumoniae, CpsB, CpsC, and CpsD are essential for encapsulation, and mutants containing deletions of cpsB, cpsC, or cpsD...Full Text Available

2003-05-01

109

Molecular characterization of cap3A, a gene from the operon required for the synthesis of the capsule of Streptococcus pneumoniae type 3: sequencing of mutations responsible for the unencapsulated phenotype and localization of the capsular cluster on the pneumococcal chromosome.  

UK PubMed Central (United Kingdom)

The complete nucleotide sequence of the cap3A gene of Streptococcus pneumoniae, which is directly responsible for the transformation of some unencapsulated, serotype 3 mutants to the encapsulated phenotype,...Full Text Available

1994-10-01

110

Induced mutation in narrow-leafed lupin improvement: An example of herbicide tolerance  

International Nuclear Information System (INIS)

Spontaneous mutation has been discovered and utilized in domestication of narrow-leafed lupin (Lupinus angustifolius L.). As the result of the domestication, lupin has become a dominant grain legume crop in Western Australia. Facing the new challenge of developing herbicide tolerance cultivars, chemical mutagenesis has been used to create new tolerance to herbicide. This paper reports the characterization of two lupin mutants (Tanjil-AZ-33 and Tanjil-AZ-55) that are highly tolerant to metribuzin herbicide. A dose response study over 8 doses revealed that Tanjil-AZ-33 was 6 times more tolerant to metribuzin than the original parental cultivar Tanjil by measure of LD50. This mutant Tanjil-AZ-33 is the most tolerant germplasm in narrow-leafed lupin. Both mutants also maintain the high resistance to the disease anthracnose as cv Tanjil. Seed yield based on small field plots (3.6 m"2) under irrigation was 4.2 t/ha for ...

2008-08-12

111

Induced mutation in Lupinus mutabilis sweet in Peru  

International Nuclear Information System (INIS)

Mutagenesis of Lupinus mutabilis was started at the UNA LM (Peru) to obtain mutants with low alkaloid content and early germination. Varieties SCG 25 and Lib 020 were irradiated with gamma radiation. The optimum dose for the SCG 25 variety was 15 Krad and for Lib 020 15 to 20 Krad. The relation between the plant height and radiation dose fits the quadratic polynomial model.

1984-04-01

112

Individual products of the adenovirus 12S and 13S EIa mRNAs stimulate viral EIIa and EIII expression at the transcriptional level.  

UK PubMed Central (United Kingdom)

Recombinant plasmids containing mutant or wild-type adenovirus serotype 2 EIa genes that produce the 12S mRNA alone, the 13S mRNA alone, or both mRNAs were cotransfected into HeLa cells with plasmids...Full Text Available

1984-07-01

113

High yielding mutants in sesamum  

International Nuclear Information System (INIS)

Mutation breeding in one of the cultigens TMV-5 in Sesamum is undertaken with 5 kr, 10 kr, 15 kr, 20 kr, 60 kr and 80 kr, dosages of gamma-rays. 60 kr and 80 kr dosages were found to be lethal from the complete failure of seed germination. The mutagen had a heterotic effect on the different characters and the promising variants obtained in this programme are proposed to be maintained by pureline breeding for their possible release as new varieties. (author).

114

Expression of V642 APP mutant causes cellular apoptosis as Alzheimer trait-linked phenotype.  

UK PubMed Central (United Kingdom)

APP is a transmembrane precursor of beta-amyloid. In dominantly inherited familial Alzheimer's disease (FAD), point mutations V6421, V642F and V642G have been discovered in APP695. Here we show that...Full Text Available

1996-02-01

115

Distribution and threshold expression of the tRNA(Lys) mutation in skeletal muscle of patients with myoclonic epilepsy and ragged-red fibers (MERRF).  

UK PubMed Central (United Kingdom)

We investigated the distribution and expression of mutant mtDNAs carrying the A-to-G mutation at position 8344 in the tRNA(Lys) gene in the skeletal muscle of four patients with myoclonus epilepsy and...Full Text Available

1992-12-01

116

Crystallization and preliminary X-ray diffraction studies of two thermostable ?-galactosidases from glycoside hydrolase family 36  

UK PubMed Central (United Kingdom)

α-Galactosidases from thermophilic organisms have gained interest owing to their applications in the sugar industry. The α-galactosidases AgaA, AgaB and AgaA A355E mutant from Geobacillus...Full Text Available

117

Complementation of areA- regulatory gene mutations of Aspergillus nidulans by the heterologous regulatory gene nit-2 of Neurospora crassa.  

UK PubMed Central (United Kingdom)

Loss-of-function mutations in the regulatory gene areA of Aspergillus nidulans prevent the utilization of a wide variety of nitrogen sources. The phenotypes of nit-2 mutants of Neurospora crassa suggest...Full Text Available

1987-06-01

118

Alteration of nucleoside diphosphate binding specificity of E. coli elongation factor Tu (EF-Tu) by single amino acid substitution at position 138  

International Nuclear Information System (INIS)

A single amino acid substitution (Asp #-># Asn) at position 138 of E. coli EF-Tu was induced in the tufA gene by an M13 phage oligonucleotide site-directed mutagenesis protocol. The mutated tufA gene was then subcloned in a plasmid vector and expressed in maxicells. The properties of ["3"5S]methionine labelled mutant and wild type EF-Tu's were compared by in vitro assays. Mutant and wild-type EF-Tu's bound EF-Ts with approximately equal affinities. The 138-Asn mutation greatly reduced the protein's affinity for GDP; however, this mutation dramatically increased the proteins affinity for XDP. The mutant protein forms a stable complex with phe-tRNA and XTP, which binds to ribosomes; whereas, it does not form a complex with phe-tRNA and GTP. These results suggest that in EF-Tu x NDP complexes amino acid residue 138 must interact with the substituent on C-2 of the purine ring. Thus in wild-type EF-Tu Asp-138 would H-bond to ...

1987-06-07

119

A deficiency in cyclic AMP results in pH-sensitive growth of Escherichia coli K-12.  

UK PubMed Central (United Kingdom)

Mutants of Escherichia coli K-12 deficient in adenyl cyclase (cya) and catabolite activator protein (crp) have been shown to grow more slowly than their parent strains in glucose-minimal medium. Their...Full Text Available

1988-08-01

120

Role of the Yersinia pestis Ail Protein in Preventing a Protective Polymorphonuclear Leukocyte Response During Bubonic Plague.  

Science.gov (United States)

The ability of Yersinia pestis to forestall the mammalian innate immune response is a fundamental aspect of plague pathogenesis. In this study, we examined the effect of Ail, a 17-kDa outer membrane protein that protects Y. pestis against complement-mediated lysis, on bubonic plague pathogenesis in mice and rats. The Y. pestis ail mutant was attenuated for virulence in both rodent models. The attenuation was greater in rats than in mice, which correlates with the ability of normal rat serum, but not mouse serum, to kill ail-negative Y. pestis in vitro. Intradermal infection with the ail mutant resulted in an atypical, subacute form of bubonic plague associated with extensive recruitment of polymorphonuclear leukocytes (PMN or neutrophils) to the site of infection in the draining lymph node and the formation of large purulent abscesses that contained the bacteria. Systemic spread and mortality were greatly attenuated, however, and a productive ...

2011-10-01

121

Long-term Retinal Function and Structure Rescue Using Capsid Mutant AAV8 Vector in the rd10 Mouse, a Model of Recessive Retinitis Pigmentosa  

British Library Electronic Table of Contents (United Kingdom)

The retinal degeneration 10 (rd10) mouse is a well-characterized model of autosomal recessive retinitis pigmentosa (RP), which carries a spontaneous mutation in the ? subunit of rod cGMP-phosphodiesterase (PDE?). Rd10 mouse exhibits photoreceptor dysfunction and rapid rod photoreceptor degeneration followed by cone degeneration and remodeling of the inner retina. Here, we evaluate whether gene replacement using the fast-acting tyrosine-capsid mutant AAV8 (Y733F) can provide long-term therapy in this model. AAV8 (Y733F)-smCBA-PDE? was subretinally delivered to postnatal day 14 (P14) rd10 mice in one eye only. Six months after injection, spectral domain optical coherence tomography (SD-OCT), electroretinogram (ERG), optomotor behavior tests, and immunohistochemistry showed tha...

2011-01-01

122

Localized mutagenesis of the tetracycline promoter region in pBR322 by 4,5',8-trimethylpsoralen  

Energy Technology Data Exchange (ETDEWEB)

In vitro mutagenesis of functional DNA gene fragments by covalently reactive agents permits one in principle to examine the consequent alterations in DNA sequence directly. I have carried out selective mutagenesis of the tetracycline resistance gene in the plasmid pBR322 using the long wavelength UV light activated reaction of 4,5',8-trimethylpsoralen (TMP). The mutagenized DNA sequence was the EcoR1-Hind III restriction fragment in the vicinity of the Tcsup(R) promoter. Two classes of mutants were obtained. One exhibited a high level of Tc resistance (40-60 ..mu..g/ml) but still lower than the wild-type. Interestingly, these showed no sequence alterations at all in the vicinity of the TMP-reacted fragment. The other class of mutants exhibited low levels of drug resistance (< 20 ..mu..g/ml) and two of those that were sequenced were found to contain a 15-base pair insertion to the right of the original Hind III site. Under the ...

1982-04-01

123

Induction of mutation in Trichoderma viride for conversion of natural cellulose into glucose  

Energy Technology Data Exchange (ETDEWEB)

The production of cellulolytic enzymes from fungi has been extensively studied. Several mutants of Trichoderma reesei were selected. Most of the studies were carried out on T. reesei, T. viride, T. harzianum, Penicillium funiculosum, Altemaria alternata. Aspergillus phoenicis, A. ustus, A. tamarii, A. japonicus, and A. niger. T. koningii is one of the most active producers of the so-called C, factor, which is indispensable for the rapid and extensive attack on crystal-line cellulose. However, Trichodenna is known to excrete only small amounts of {beta}-glucosidase. Therefore, Trichoderma is supplemented with {beta}-glucosidase from Aspergillus to increase the saccharification rate of cellulose to glucose as the main sugar. Induction of mutations in Trichodenna spp. rather than T. viride as a tool for the enhancement of {beta}-glucosidase activity was reported. Unfortunately, T. reesei is a poor producer of {beta}-glucosidase. On the other hand, T. harzianum M{sub ...

1991-12-31

124

Hemoglobin of mice with radiation-induced mutations at the hemoglobin loci  

International Nuclear Information System (INIS)

Chemical analyses were done on the abnormal hemoglobins of the five (101 x SEC)F_1 offspring of X- irradiated adult SEC mice to determine which hemoglobin genes were expressed in each hemoglobin variant. Three offspring of irradiated SEC males did not express either of the two kinds of #alpha#-chains normally found in all SEC mice. The deficient #alpha#-chain synthesis caused these mice to exhibit an #alpha#-thalassemia similar to human #alpha#-thalassemia. Scanning electron microscopy was used to show that many erythrocytes of mice with #alpha#-thalassemia have bizarre shapes; e.g. many erythrocytes appeared flattened or had thorny projections (acanthocytes). One mutant with a tandem duplication of a segment of chromosome 7 (site of locus determining #beta#-chain structure) produced twice as much SEC as 101 #beta#-chain polypeptides. One mutant that probably arose by non-disjunction of chromosome 7's in its unirradiated 101 mother and loss of ...

125

GROWTH REGULATION IN ROUS SARCOMA VIRUS INFECTED CHICKEN EMBRYO FIBROBLASTS: THE ROLE OF THE src GENE  

Energy Technology Data Exchange (ETDEWEB)

We report here a study of the mechanisms leading to loss of growth control in chicken embryo fibroblasts transformed by Rous sarcoma virus (RSV). We have been particularly concerned with the role of the src gene in this process, and have used RSV mutants temperature sensitive (ts) for transformation to investigate the nature of the growth regulatory lesion. The two principal findings were (1) the stationary phase of the cell cycle (G{sub 1}) in chick embryo fibroblasts seems to have two distinct regulatory compartments (using the terminology of Brooks et al. we refer to these as 'Q' and 'A' states). When rendered stationary at 41.5 C by serum deprivation, normal cells enter a Q state, but cells infected with the ts-mutant occupy an A state. (2) Whereas normal cells can occupy either state depending on culture conditions, the ts-infected cells, at 41.5 C, do not seem to enter Q even though a known src gene ...

1980-07-01

126

Endonuclease IV of Escherichia coli is induced by paraquat  

International Nuclear Information System (INIS)

The addition of paraquat (methyl viologen) to a growing culture of Escherichia coli K-12 led within 1 hr to a 10- to 20-fold increase in the level of endonuclease IV, a DNase for apurinic/apyrimidinic sites. The induction was blocked by chloramphenicol. Increases of 3-fold or more were also seen with plumbagin, menadione, and phenazine methosulfate. H_2O_2 produced no more than a 2-fold increase in endonuclease IV activity. The following agents had no significant effect: streptonigrin, nitrofurantoin, tert-butyl hydroperoxide, #gamma# rays, 260-nm UV radiation, methyl methanesulfonate, mitomycin C, and ascorbate. Paraquat, plumbagin, menadione, and phenazine methosulfate are known to generate superoxide radical anions via redox cycling in vivo. A mutant lacking superoxide dismutase was unusually sensitive to induction by paraquat. In addition, endonuclease IV could be induced by merely growing the mutant in pure O_2. The levels of endonuclease ...

127

Ectopic expression of wild-type or a dominant-negative mutant of transcription factor NTF-1 disrupts normal Drosophila development.  

Science.gov (United States)

The Drosophila melanogaster tissue-specific transcription factor NTF-1 was originally identified in vitro as a protein that could bind to and activate transcription from the Dopa decarboxylase (Ddc) gene. A structure-function analysis of NTF-1 led to the identification of a discrete amino-terminal activation domain. Here, we report that an NTF-1 mutant lacking the activation domain acts as a trans-dominant inhibitor of NTF-1 activation in tissue culture cells by forming inactive heterodimers with the full-length protein. Ectopically expressing this dominant-negative protein or the full-length protein in developing Drosophila embryos leads to dire developmental consequences. Overexpressing the trans-dominant NTF-1 leads to lethality, while overexpressing full-length NTF-1 results in both lethality and morphogenetic defects. Our results suggest that both the activity and the regulation of NTF-1 are critical for viability and proper development of the fly.ImagesFig. ...

1993-11-15

128

Development of a novel mouse tk{sup +/-} embryonic stem cell line for use in mutagenicity studies  

Energy Technology Data Exchange (ETDEWEB)

A tk{sup +/-} mouse embryonic stem (ES) cell line, designated 1G2, has been created in which one allele of the thymidine kinase (tk) gene was inactivated by targeted homologous recombination. This line is an analog of the mouse lymphoma tk{sup +/-} L5178Y cell line, which is used widely to assess the mutagenicity of chemical agents. Treatment of 1G2 cells with the alkylating agent N-ethyl-N-nitrosourea (ENU) resulted in a dose-related increase in tribluorothymidine-resistant colonies. Mutant frequencies of 152 and 296 per 10{sup 6} cells were determined for 0.1 and 0.3 mg/ml doses of ENU, compared with a spontaneous mutant frequency of 15 per 10{sup 6} cells. The data indicate that tk{sup +/-} 1G2 ES cells may be useful for the creation of a transgenic mouse model for assessing in vivo mutation using an endogenous autosomal gene. 45 refs., 2 figs., 1 tab.

1996-12-31

129

Methods to improve the fertility of poinsettia  

British Library Electronic Table of Contents (United Kingdom)

`Success' or `Red Splendor' plants propagated by shoot tip culture or embryogenesis had higher pollen germination than those propagated by cutting. All clones of `Pepride', `Peter Star' and `Nobel Star' propagated by cutting or by embryogenesis or shoot tip culture did not have viable pollen. `Peter Star' mutants through mutagenesis could recover pollen viability. When both parents were propagated by tissue culture, fruit set increased compared to when one or both parents were propagated by cuttings. Furthermore, the number of the harvested seeds doubled per pollination set, when the tissue culture parents were used instead of cutting-propagation plants.

2008-01-01

130

Emulsans. [Acinetobacter sp. ATCC 31012  

Energy Technology Data Exchange (ETDEWEB)

The production and uses are described for extracellular microbial polysaccharides (generically called emulsans) and for a new class of extracellular microbial protein-associated lipopolysaccharides (collectively called alpha-emulsans) produced by Acinetobacter Sp. ATCC 31012, its mutants or recombinants. Also included are the deproteinized lipopolysaccharides (collectively called apoemulsans) obtained from such emulsans, as well as to the divalent metal, ammonium, and quaternary ammonium salts of such emulsans and apoemulsans. The materials can be used (1) in cleaning oil-contaminated vessels used to transport or store crude oil or petroleum fractions in such a manner that the residual oil may be recovered for fuel value or for refining and (2) in enhanced oil recovery.

1983-07-05

131

Analysis of the tet repressor-operator interactions using the uracil-DNA glycosylase footprinting system  

Energy Technology Data Exchange (ETDEWEB)

The tet repressor regulated expression of the Tn-10-encoded tetracycline resistance determinant in a tetracycline-dependent manner. In the absence of tetracycline, the tet repressor binds as a dimer to the 19-base-pair palindromic tet operator sequence. Amino acid homologies and genetic studies with trans-dominant mutants suggest that sequence-specific recognition of the tet operator involves the extensively studied helix-turn-helix motif. We have used the uracil-DNA glycosylase (UDG) footprinting systems to identify thymine contacts in the tet operator that are essential for the formation of tet repressor-operator complexes.

1994-12-31

132

Research work on mutation breeding in Egypt during the 1980s  

International Nuclear Information System (INIS)

The research work carried out on mutation breeding in Egypt during the 1980s is summarized. Several mutations have been developed in bread wheat, maize, rice and barley. A higher yield, tolerance to salinity, shorter types and earliness were obtained after use of different mutagens and growth regulators. Great attention has been paid to the fababean and chickpea, particularly in improving their quality and quantity of protein, and their resistance to insect weevils such as Callosobruchus sp. Tolerance or resistance to broom rape has also been reported. Various grain legumes such as lentil, pea, cowpea, bean, fenugreek and lupin received some attention. Mutation work on fibre crops such as cotton, kenaf and flax has led to some promising results. Zero type, glandless and early maturing mutants were obtained in cotton, and early flowering, high yielding (fibre or oil) mutants in flax. Some attention has been given to oil crops such as sesame, ...

1990-06-18

133

Synthetic Biology and Human Health: Potential Applications for Spaceflight  

Science.gov (United States)

Human space travelers experience a unique environment that affects homeostasis and physiologic

2011-01-01

134

Spatial Variations in the Catch of Billfishes in the Pacific Ocean and Factors Affecting Annual Changes in the Catch  

KoreaScience (Korea)

Full Text Available

2009-12-01

135

School based intervention improves PTSD symptoms in children affected by political violence.  

Science.gov (United States)

PMID:19395606

2009-05-01

136

Promoter Structure Which Affects on the Expression of Yeast MGMT Gene  

KoreaScience (Korea)

Full Text Available

1997-01-01

137

Dept. of Labor  

Science.gov (United States)

... assistance for Iowa workers affected by layoffs at Electrolux [10/17/2011] DOL provides grant increment to ...

138

Affect School for chronic benign pain patients showed improved alexithymia assessments with TAS-20.  

Science.gov (United States)

PMID:20525319

2010-06-04

139

The language of pain: affective descriptors of pain are a better predictor of psychological disturbance than pattern of sensory and affective descriptors.  

Science.gov (United States)

The language used by chronic benign pain patients to characterize their pain complaint was analyzed to determine the best predictor of psychiatric disturbance. Using the 78 adjectives provided by the McGill Pain Questionnaire, the number of affective descriptors used was the best predictor of psychiatric disturbance. Addition of sensory descriptors either to augment the total number of descriptors used (magnitude) or as a pattern of sensory greater than affective or sensory less than affective failed to increase predictive strength. PMID:6877847

1983-06-01

140

Pain measurement: the affective dimensional measure of the McGill pain questionnaire with a cancer pain population.  

Science.gov (United States)

Two experiments used the McGill Pain Questionnaire (MPQ) to examine the affective dimension of pain in patients whose pain was secondary to malignancy. In experiment I, segregating groups of cancer patients on the basis of extreme scores (high versus low) on the MPQ failed to produce segregation on independent measures of affect and infirmity. This outcome contrasts with earlier work with chronic benign pain patients. Experiment II compared cancer pain patients matched with benign pain patients on intensity of pain report on the affective dimension of the MPQ. Cancer pain patients reported a reliably higher affective loading to their pain. These data suggest that cancer pain patients employ different criteria than benign pain patients in selecting affective pain descriptors. Possible explanations for this difference are discussed. PMID:7070825

1982-02-01

141

Flexible responses to visual and olfactory stimuli by foraging Manduca sexta: larval nutrition affects adult behaviour  

UK PubMed Central (United Kingdom)

Here, we show that the consequences of deficient micronutrient (β-carotene) intake during larval stages of Manduca sexta are carried across metamorphosis, affecting adult behaviour....Full Text Available

2009-08-07

142

Factors affecting the aggregation of Actinomyces naeslundii during growth and in washed cell suspensions.  

UK PubMed Central (United Kingdom)

Various factors affecting the aggregation of Actinomyces naeslundii strain 12104 were studied. When the pH of glucose-supplemented growth medium fell below 5.5, the cells aggregated and formed microbial...Full Text Available

1978-09-01

143

Factors affecting birth weight in sheep: maternal environment  

UK PubMed Central (United Kingdom)

Knowledge of factors affecting variation in birth weight is especially important given the relationship of birth weight to neonatal and adult health. The present study utilises two large contemporary...Full Text Available

2007-01-01

144

[Destruction of radioactive particles by strains of Cladosporium cladosporoides (FRES.) de Vries].  

Science.gov (United States)

Reactions on the ionizing radiation of 14 Cladosporium cladosporioides strains were studied. Only 5 of them displayed radiotropizm. The ability of C. cladosporioides strains 4 and 5 with positive radiotropizm and museum C. cladosporioides strain 396 and its alb-mutant SM without positive radiotropizm to destruct radioactive particles of Chernobyl and explosion origin was studied. Two ways of radioactive particles destruction by C. cladosporioides were established, one of them is a direct way by fungal overgrowth of hot particles and the second one an indirect way only by fungal metabolites. Mycelium of the studied C. cladosporioides strains sorbed radionuclides from radioactive particles during cultivation on the liquid and agarized media. No certain inclinations of the individual strains to accumulation of radionuclides 137Cs or 152Eu were ascertained. PMID:12664550

145

Wnt/b-catenin signal pathway stabilizes APP intracellular domain (AICD) and promotes its transcriptional activity  

British Library Electronic Table of Contents (United Kingdom)

Amyloid precursor protein (APP), a key protein in pathogenesis of Alzheimer's disease (AD), is a type I transmembrane protein which can be cleaved by b- and g-secretase to release the amyloidogenic b-amyloid peptides (Ab) and the APP intracellular domain (AICD). While Ab has been widely believed to initiate pathogenic cascades culminating AD, the physiological functions and regulations of AICD remain elusive. In present study, endogenous AICD was demonstrated to be increased by canonical Wnt signal. Instead of due to g-secretase activity, enhanced AICD expression was found due to the increased protein stability by Wnt/b-catenin. b-Catenin was demonstrated to be an associating partner of AICD, capable of promoting AICD mediated transcriptional activity. Investigation by AICD mutants proved ...

2011-01-01

146

Unfolding analysis of the mature and unprocessed forms of Bacillus licheniformis ?-glutamyltranspeptidase  

British Library Electronic Table of Contents (United Kingdom)

Bacillus licheniformis ?-glutamyltranspeptidase (BlGGT) undergoes an autocatalytic process to generate 44.9 and 21.7?kDa subunits; however, a mutant protein (T399A) loses completely the processing ability and mainly exists as a precursor. For a comprehensive understanding of their structural features, the biophysical properties of these two proteins were investigated by circular dichroism and fluorescence spectroscopy. Tryptophan fluorescence and circular dichroism spectra were nearly identical for BlGGT and T399A, but unfolding analyses revealed that these two proteins had a different sensitivity towards temperature- and guanidine hydrochloride (GdnHCl)-induced denaturation. BlGGT and the unprocessed T399A displayed T m values of 61.4?C and 68.1?C, respectively, and thermal unfolding of b...

2011-01-01

147

UV inactivation: Combined effects of UV radiation and xenobiotics in two strains of Saccharomyces  

International Nuclear Information System (INIS)

The effects of eight chemicals on the inactivation rate of ultraviolet radiation on the colony building capabilities of two strains of Saccharomyces cervisae - a wild type strain and a mutant deficient in excision repair - were studied. The insecticide methoxychlor, the herbicide 2,4-dichlorophenoxyacetic acid, the fungicide pentachlorophenol and its metabolite tetrachlorohydroquinone, as well as the chemicals acrylonitrile and 2,3-dichloro-1-propene have no significant impact on the effects of UV radiation in Saccharomyces cerevisae. Depending on the concentration, trichloroethylene increases the sensitivity to UV radiation. The herbicide paraquat provides efficient protection against UV radiation at concentrations where a toxic effect cannot be observed even without UV. The results were rather similar for both strains. (orig.).

148

Transformation by complementation of an adenine auxotroph of the lignin-degrading basidiomycete Phanerochaete chrysosporium  

Science.gov (United States)

Swollen basiodiospores of an adenine auxotroph of Phanerochaete chrysosporium were protoplasted with Novozyme 234 and transformed to prototrophy by using a plasmid containing the gene for an adenine biosynthetic enzyme from Schizophyllum commune. Transformation frequencies of 100 transformants per {mu}g of DNA were obtained. Southern blot analysis of DNA extracted from transformants demonstrated that plasmid DNA was integrated into the chromosomal DNA in multiple tandem copies. Analysis of conidia and basiodiospores from transformants demonstrated that the transforming character was mitotically and meiotically stable on both selective and nonselective media. Genetic crosses between double mutants transformed for adenine prototrophy and other auxotrophic strains yielded Ade{sup {minus}} progeny, which indicated that integration occurred at a site(s) other than the resident adenine biosynthetic gene.

1989-02-01

149

The type III secretion system is involved in Escherichia coli K1 interactions with Acanthamoeba  

British Library Electronic Table of Contents (United Kingdom)

The type III secretion system among Gram-negative bacteria is known to deliver effectors into host cell to interfere with host cellular processes. The type III secretion system in Yersina, Pseudomonas and Enterohemorrhagic Escherichia coli have been well documented to be involved in the bacterial pathogenicity. The existence of type III secretion system has been demonstrated in neuropathogenic E. coli K1 strains. Here, it is observed that the deletion mutant of type III secretion system in E. coli strain EC10 exhibited defects in the invasion and intracellular survival in Acanthamoeba castellanii (a keratitis isolate) compared to its parent strain. Next, it was determined whether type III secretion system plays a role in E. coli K1 survival inside Acanthamoeba during the encystment process...

2011-01-01

150

R46 and pKM101 plasmid-mediated resistance to ionizing radiation in Escherichia coli  

International Nuclear Information System (INIS)

The ability of the R46 R factor and its derivative pKM101 to modify sensitivity to "6"0Co #gamma# radiation was studied. In Escherichia coli K12 both plasmids enhanced bacterial survival after "6"0Co #gamma# irradiation. This effect was dependent on recA"+ genotype but not on recB"+, recB"+recC"+, and recF"+ genotypes. 5-Fluorouracil eliminated the R46 R factor from the parent and its rec"- mutant strains. These strains lost not only the antibiotic resistance coded for R46 R factor but their radioresistance as well.

151

Pigpen, a nuclear coiled body component protein, is involved in angiogenesis  

British Library Electronic Table of Contents (United Kingdom)

We previously reported that puromycin-insensitive leucyl-specific aminopeptidase (PILSAP) is required for vascular endothelial growth factor (VEGF)- and basic fibroblast growth factor (bFGF)-induced angiogenesis and for endothelial differentiation from embryonic stem (ES) cells via the aminopeptidase activity of PILSAP. In this study, we searched for molecules that function during angiogenesis with PILSAP. We performed proteome analysis of nuclear extracts from embryoid bodies (EBs) made from ES cells transfected with mutant PILSAP lacking aminopeptidase activity and mock EBs. We identified pigpen, a 67-kDa nuclear coiled body component protein. Immunoprecipitation and western blotting demonstrated the binding of PILSAP and pigpen in endothelial cells (ECs), and this interaction was enhanc...

2010-01-01

152

Photosynthetic energy storage efficiency in Chlamydomonas reinhardtii, based on microsecond photoacoustics  

British Library Electronic Table of Contents (United Kingdom)

Using a novel, pulsed micro-second time-resolved photoacoustic (PA) instrument, we measured thermal dissipation and energy storage (ES) in the intact cells of wild type (WT) Chlamydomonas reinhardtii, and mutants lacking either PSI or PSII reaction centers (RCs). On this time scale, the kinetic contributions of the thermal expansion component due to heat dissipation of absorbed energy and the negative volume change due to electrostriction induced by charge separation in each of the photosystems could be readily distinguished. Kinetic analysis revealed that PSI and PSII RCs exhibit strikingly different PA signals where PSI is characterized by a strong electrostriction signal and a weak thermal expansion component while PSII has a small electrostriction component and large thermal expansion....

2011-01-01

153

Phenotyping in the archaea: optimization of growth parameters and analysis of mutants of Haloferax volcanii  

British Library Electronic Table of Contents (United Kingdom)

Abstract A method to grow the halophilic archaeon Haloferax volcanii in microtiter plates has been optimized and now allows the parallel generation of very reproducible growth curves. The doubling time in a synthetic medium with glucose is around 6-h. The method was used to optimize glucose and casamino acid concentrations, to clarify carbon source usage and to analyze vitamin dependence. The characterization of osmotolerance revealed that after a lag phase of 24-h, H. volcanii is able to grow at salt concentrations as low as 0.7-M NaCl, much lower than the 1.4-M NaCl described as the lowest concentration until now. The application of oxidative stresses showed that H. volcanii exhibits a reaction to paraquat that is delayed by about 10-h. Surprisingly, only one of two amino acid auxotrophi...

2011-01-01

154

One-step RNA pathogen detection with reverse transcriptase activity of a mutated thermostable Thermus aquaticus DNA polymerase  

British Library Electronic Table of Contents (United Kingdom)

We describe the cloning and characterization of a mutated thermostable DNA polymerase from Thermus aquaticus (Taq) that exhibits an increased reverse transcriptase activity and is therefore designated for one-step PCR pathogen detection using established real-time detection methods. We demonstrate that this Taq polymerase mutant (Taq M1) has similar PCR sensitivity and nuclease activity as the respective Taq wild-type DNA polymerase. In addition, and in marked contrast to the wild-type, Taq M1 exhibits a significantly increased reverse transcriptase activity especially at high temperatures (>60degreeC). RNA generally hosts highly stable secondary structure motifs, such as hairpins and G-quadruplexes, which complicate, or in the worst case obviate, reverse transcription (RT). Thus, RT at hi...

2010-01-01

155

N-acetoxy-N-2-acetylaminofluorene induced frameshift mutations: a comparison between the DNA modification spectrum and the mutation spectrum  

International Nuclear Information System (INIS)

We describe the analysis of forward mutations induced in the tetracycline resistance gene of the plasmid pBR322 by directing the reaction of the carcinogen N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) to a small restriction fragment (BamHI, SalI) that is located in the proximal part of the antibiotic-resistance gene. Mutant plasmids obtained both in wild type and excision repair deficient (uvrA) bacterial cells are compared. Preliminary data showing the distribution of the -AAF adducts along this restriction fragments are discussed in relation to the observed spectrum of mutations. 20 references, 4 figures.

156

Mutation breeding in leguminous crop plants  

Energy Technology Data Exchange (ETDEWEB)

Since it was introduced in the early 1940's, mutation breeding has been tested on many crops as modern plant breeding. Until now, more than seven hundred varieties have been developed by means of induced mutation, and many of them officially released and approved for registration. Hundreds of papers report the results of mutation breeding, and the characteristics of induced mutation in different kinds of crops were discussed for review purposes (Blixt and Gottschalk 1975, Gottschalk and Wolff 1983). Considering the results already obtained, it can be concluded that the kinds of induced mutation and their utilization vary from crop to crop. This paper summarizes and discusses the mutation characteristics and kinds on the induced mutants of leguminous plants that have been released.

1988-03-01

157

Mitochondrial DNA background modulates the assembly kinetics of OXPHOS complexes in a cellular model of mitochondrial disease  

British Library Electronic Table of Contents (United Kingdom)

Lebers hereditary optic neuropathy (LHON), the most frequent mitochondrial disorder, is mostly due to three mitochondrial DNA (mtDNA) mutations in respiratory chain complex I subunit genes: 3460/ND1, 11778/ND4 and 14484/ND6. Despite considerable clinical evidences, a genetic modifying role of the mtDNA haplogroup background in the clinical expression of LHON remains experimentally unproven. We investigated the effect of mtDNA haplogroups on the assembly of oxidative phosphorylation (OXPHOS) complexes in transmitochondrial hybrids (cybrids) harboring the three common LHON mutations. The steady-state levels of respiratory chain complexes appeared normal in mutant cybrids. However, an accumulation of low molecular weight subcomplexes suggested a complex I assembly/stability defect, which was ...

2008-01-01

158

Mannose-specific interaction of Lactobacillus plantarum with porcine jejunal epithelium  

British Library Electronic Table of Contents (United Kingdom)

Abstract Host-microorganism interactions in the intestinal tract are complex, and little is known about specific nonpathogenic microbial factors triggering host responses in the gut. In this study, mannose-specific interactions of Lactobacillus plantarum 299v with jejunal epithelium were investigated using an in situ pig Small Intestinal Segment Perfusion model. The effects of L. plantarum 299v wild-type strain were compared with those of two corresponding mutant strains either lacking the gene encoding for the mannose-specific adhesin (msa) or sortase (srtA; responsible for anchoring of cell surface proteins like Msa to the cell wall). A slight enrichment of the wild-type strain associated with the intestinal surface could be observed after 8 h of perfusion when a mixture of wild-type and...

2008-01-01

159

Levels of mesenchymal FGFR2 signaling modulate smooth muscle progenitor cell commitment in the lung  

British Library Electronic Table of Contents (United Kingdom)

Fibroblast growth factor (FGF) signaling has been shown to regulate lung epithelial development but its influence on mesenchymal differentiation has been poorly investigated. To study the role of mesenchymal FGF signaling in the differentiation of the mesenchyme and its impact on epithelial morphogenesis, we took advantage of Fgfr2c+/? mice, which due to a splicing switch express Fgfr2b in mesenchymal tissues and manifest Apert syndrome-like phenotypes. Using a set of in vivo and in vitro studies, we show that an autocrine FGF10?FGFR2b signaling loop is established in the mutant lung mesenchyme, which has several consequences. It prevents the entry of the smooth muscle progenitors into the smooth muscle cell (SMC) lineage and results in reduced fibronectin and elastin deposition. Levels of...

2006-01-01

160

Ionizing radiation is a potent inducer of mitotic recombination in mouse embryonic stem cells  

British Library Electronic Table of Contents (United Kingdom)

Maintenance of genomic integrity in embryonic cells is pivotal to proper embryogenesis, organogenesis and to the continuity of species. Cultured mouse embryonic stem cells (mESCs), a model for early embryonic cells, differ from cultured somatic cells in their capacity to remodel chromatin, in their repertoire of DNA repair enzymes, and in the regulation of cell cycle checkpoints. Using 129XC3HF1 mESCs heterozygous for Aprt, we characterized loss of Aprt heterozygosity after exposure to ionizing radiation. We report here that the frequency of loss of heterozygosity mutants in mESCs can be induced several hundred-fold by exposure to 5-10Gy of X-rays. This induction is 50-100-fold higher than the induction reported for mouse adult or embryonic fibroblasts. The primary mechanism underlying the...

2011-01-01

161

Inhibition of prepenetration processes of the powdery mildew Golovinomyces orontii on host inflorescence stems is reduced in the Arabidopsis cuticular mutant cer3 but not in cer1  

British Library Electronic Table of Contents (United Kingdom)

The obligate biotrophic fungus Golovinomyces orontii causes powdery mildew (PM) disease on its host Arabidopsis thaliana. Most research on the G. orontii?Arabidopsis pathosystem uses rosette leaves as experimental materials, so little is known about the behavior of G. orontii on other Arabidopsis organs. We thus conducted microscopic analyses of the PM infection process on leaves, stems, fruits and roots of Arabidopsis. Adaxial and abaxial surfaces of mature rosette leaves supported G. orontii infection, but growth was somewhat suppressed on cauline leaves. Prepenetration processes (germination and appressorium formation) were strongly inhibited on stems, fruits and roots. To test the effect of alterations in the Arabidopsis cuticle on the inhibition of prepenetration processes on stems, w...

2011-01-01

162

Inhibition of heat shock protein 27 (HspB1) tumorigenic functions by peptide aptamers  

British Library Electronic Table of Contents (United Kingdom)

Human heat shock protein 27 (Hsp27, HspB1) is an anti-apoptotic protein characterized for its tumorigenic and metastatic properties, and now referenced as a major therapeutic target in many types of cancer. Hsp27 biochemical properties rely on a structural oligomeric and dynamic organization. Downregulation by small interfering RNA or inhibition with dominant-negative mutant have proven their efficiency to counteract the anti-apoptotic and protective properties of Hsp27. In this study, we report the isolation and characterization of Hsp27-targeted molecules interfering with its structural organization. Using the peptide aptamer (PA) strategy, we isolated PAs that specifically interact with Hsp27 and not with the other members of the small heat shock protein family. In mammalian cell cultur...

2011-01-01

163

Improving protein quality of soybean through induced mutations  

International Nuclear Information System (INIS)

Soybean is one of the most economical and nutritious food packed with basic nutrients that combat diseases stemming from mal- and under-nutrition. Despite its rich nutritional profile, use of soybean in food has been limited because soybean proteins are often associated with compounds, which could exert a negative impact on the nutritional quality of the protein. Trypsin inhibitor (TI) is one of the important anti-nutritional factors that exert negative effect by causing growth inhibition. Soybean cultivar VLS-2 was irradiated with 250 Gy gamma rays in a gamma cell (200) with 60Co source installed at BARC to induce mutations for low trypsin inhibitor content. Three mutants with lower levels of TI content were identified and can be utilized for developing elite varieties of soybean. (author)

2011-02-22

164

Identification of a functional nuclear export signal in the green fluorescent protein asFP499  

International Nuclear Information System (INIS)

The green fluorescent protein (GFP) asFP499 from Anemonia sulcata is a distant homologue of the GFP from Aequorea victoria. We cloned the asFP499 gene into a mammalian expression vector and showed that this protein was expressed in the human lymphoblast cell line Ramos RA1 and in the embryonic kidney 293T cell line (HEK 293T). In HEK 293T cells, asFP499 was localized mainly in the cytoplasm, suggesting that the protein was excluded from the nucleus. We identified _1_9_4LRMEKLNI_2_0_1 as a candidate nuclear export signal in asFP499 and mutated the isoleucine at position 201 to an alanine. Unlike the wildtype form, the mutant protein was distributed throughout the cytoplasm and nucleus. This is First report of a GFP that contains a functional NES.

2006-04-21

165

Heparin binding sites on Ross River virus revealed by electron cryo-microscopy  

International Nuclear Information System (INIS)

Cell surface glycosaminoglycans play important roles in cell adhesion and viral entry. Laboratory strains of two alphaviruses, Sindbis and Semliki Forest virus, have been shown to utilize heparan sulfate as an attachment receptor, whereas Ross River virus (RRV) does not significantly interact with it. However, a single amino acid substitution at residue 218 in the RRV E2 glycoprotein adapts the virus to heparan sulfate binding and expands the host range of the virus into chicken embryo fibroblasts. Structures of the RRV mutant, E2 N218R, and its complex with heparin were determined through the use of electron cryo-microscopy and image reconstruction methods. Heparin was found to bind at the distal end of the RRV spikes, in a region of the E2 glycoprotein that has been previously implicated in cell-receptor recognition and antibody binding.

2005-02-20

166

Generation of N-ethyl-N-nitrosourea-induced mouse mutants with deviations in hematological parameters  

British Library Electronic Table of Contents (United Kingdom)

Research on hematological disorders relies on suitable animal models. We retrospectively evaluated the use of the hematological parameters hematocrit (HCT), hemoglobin (HGB), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), red blood cell count (RBC), white blood cell count (WBC), and platelet count (PLT) in the phenotype-driven Munich N-ethyl-N-nitrosourea (ENU) mouse mutagenesis project as parameters for the generation of novel animal models for human diseases. The analysis was carried out on more than 16,000 G1 and G3 offspring of chemically mutagenized inbred C3H mice to detect dominant and recessive mutations leading to deviations in the levels of the chosen parameters. Identification of animals exhibiting altered valu...

2011-01-01

167

Functional domain analysis of glass, a zinc-finger-containing transcription factor in Drosophila.  

Science.gov (United States)

The glass gene is required for proper photo-receptor differentiation during development of the Drosophila eye glass codes for a DNA-binding protein containing five zinc fingers that we show is a transcriptional activator. A comparison of the sequences of the glass genes from two species of Drosophila and a detailed functional domain analysis of the Drosophila melanogaster glass gene reveal that both the DNA-binding domain and the transcriptional-activation domain are highly conserved between the two species. Analysis of the DNA-binding domain of glass indicates that the three carboxyl-terminal zinc fingers alone are necessary and sufficient for DNA binding. We also show that a deletion mutant of glass containing only the DNA-binding domain can behave in a dominant-negative manner both in vivo and in a cell culture assay that measures transcriptional activation. PMID:7604032

1995-07-01

168

BzpF is a CREB-like transcription factor that regulates spore maturation and stability in Dictyostelium  

British Library Electronic Table of Contents (United Kingdom)

The cAMP response element-binding protein (CREB) is a highly conserved transcription factor that integrates signaling through the cAMP-dependent protein kinase A (PKA) in many eukaryotes. PKA plays a critical role in Dictyostelium development but no CREB homologue has been identified in this system. Here we show that Dictyostelium utilizes a CREB-like protein, BzpF, to integrate PKA signaling during late development. bzpF^- mutants produce compromised spores, which are extremely unstable and germination defective. Previously, we have found that BzpF binds the canonical CRE motif in vitro. In this paper, we determined the DNA binding specificity of BzpF using protein binding microarray (PBM) and showed that the motif with the highest specificity is a CRE-like sequence. BzpF is necessary to ...

2011-01-01

169

Bacteriophage P22 capsid size determination: Roles for the coat protein telokin-like domain and the scaffolding protein amino-terminus  

British Library Electronic Table of Contents (United Kingdom)

Assembly of icosahedral capsids of proper size and symmetry is not understood. Residue F170 in bacteriophage P22 coat protein is critical for conformational switching during assembly. Substitutions at this site cause assembly of tubes of hexamerically arranged coat protein. Intragenic suppressors of the ts phenotype of F170A and F170K coat protein mutants were isolated. Suppressors were repeatedly found in the coat protein telokin-like domain at position 285, which caused coat protein to assemble into petite procapsids and capsids. Petite capsid assembly strongly correlated to the side chain volume of the substituted amino acid. We hypothesize that larger side chains at position 285 torque the telokin-like domain, changing flexibility of the subunit and intercapsomer contacts. Thus, a sing...

2011-01-01

170

Application of laser fluorimetry for determining the influence of a single amino-acid substitution on the individual photophysical parameters of a fluorescent form of a fluorescent protein mRFP1  

International Nuclear Information System (INIS)

Individual photophysical parameters of the chromophore of a fluorescent protein mRFP1 and its two mutants (amino-acid substitution at position 66 - mRFP1/ Q66C and mRFP1/Q66S proteins) are determined. For this purpose, apart from conventional methods of fluorimetry and spectrophotometry, nonlinear laser fluorimetry is used. It is shown that the individual extinction coefficients of the chromophore of proteins correlate (correlation coefficient above 0.9) with the volume of the substituted amino-acid residue at position 66 (similar to the positions of the absorption, fluorescence excitation and emission maxima). (laser biology and medicine)

2009-03-31

171

A systematic RNAi screen reveals involvement of endocytic pathway in neuronal dysfunction in a-synuclein transgenic C. elegans  

British Library Electronic Table of Contents (United Kingdom)

Mutations or multiplications in a-synuclein gene cause familial forms of Parkinson disease or dementia with Lewy bodies (LB), and the deposition of wild-type a-synuclein as LB occurs as a hallmark lesion of these disorders, collectively referred to as synucleinopathies, implicating a-synuclein in the pathogenesis of synucleinopathy. To identify modifier genes of a-synuclein-induced neurotoxicity, we conducted an RNAi screen in transgenic C. elegans (Tg worms) that overexpress human a-synuclein in a pan-neuronal manner. To enhance the RNAi effect in neurons, we crossed a-synuclein Tg worms with an RNAi-enhanced mutant eri-1 strain. We tested RNAi of 1673 genes related to nervous system or synaptic functions, and identified 10 genes that, upon knockdown, caused severe growth/motor abnormalit...

2008-01-01

172

Transfection of endothelial cells with EJ-ras oncogene affects cell cycle and the expression of hyaluronic acid, syndecan-4, epimerase and sulfotransferases  

Energy Technology Data Exchange (ETDEWEB)

No abstract prepared.

2005-07-01

173

Measurement of Rainfall Characteristics and Rain-Attenuation at 38 GHz in Worst Months Affected by El Nino Signal in 1998  

KoreaScience (Korea)

Full Text Available

2005-12-01

174

Factors Affecting the Environmental Stability of Laminates,  

Science.gov (United States)

... The moisture content-dimension relationship of two laminate components, phenolic core and melamine overlay, are compared. (Author, modified). ...

1967-02-08

175

Congenital Adrenal Hyperplasia  

Medline Plus

... Resources and Links Kid's Newsletter Listserv's Grandparent's Newsletter Networking Personal Stories by Affected Kids & Adults (currently not ...

176

/_// ;_/ AN INTERIM OVERVIEW OF LDEF MATERIALS FINDINGS  

Science.gov (United States)

FEP polyethylene mechanical properties affected by UV. " Siloxane-modified materials resist AO. " Nonsilicone polymers attacked by AO ...

177

Effects of nitrogen fertilization on forest trees in relation to insect resistance and to red-listed insect species  

Energy Technology Data Exchange (ETDEWEB)

Ecosystems worldwide are experiencing unprecedented nitrogen enrichment through fertilization and pollution. While longterm ecological consequences are difficult to predict, it seems that plants and animals adapted to nitrogen-limited environments are at particular risk from these changes. This report summarizes the limited body of literature which addresses this important topic. From a herbivoreAes perspective, fertilization increases the nutritional quality of host plant tissues. In some cases fertilization has lead to decreased production of defensive compounds. How this affects populations of insects is unclear because fertilization affects not only herbivores but their natural enemies. This report outlines how fertilization affects tree processes such as growth, photosynthesis, and production of defensive compounds. The many factors that affect insect repsonse to fertilization and the difficulties ...

2001-10-01

178

Ionizing radiation-induced mutation of human cells with different DNA repair capacities  

Energy Technology Data Exchange (ETDEWEB)

We have observed significant differences in the response to ionizing radiation of two closely related human cell lines, and now compare the effects on these lines of both low and intermediate LET radiation. Compared to TK6, WTK1 has an enhanced X-ray survival, and is also more resistant to cell killing by {alpha}-particles. The hprt locus is more mutable in WTK1 than in TK6 by both X-rays and {alpha}-particles. WTK1 is also more mutable by {alpha}-particles than by X-rays at the hprt locus. X-ray-induced mutation at the heterozygous tk locus in WTK1 is about 25 fold higher than in TK6, while {alpha}-particle-induced mutation is nearly 50 fold higher at this locus. Also, the slowly growing tk- mutants, which comprise the majority of spontaneous and X-ray-induced tk- mutants of TK6, were not induced significantly by {alpha}-particles. Previously, we showed that TK6 has a reduced capacity for recombination compared with WTK1, and therefore, these ...

1994-12-31

179

Apoptotic pathways as regulators of recombination  

International Nuclear Information System (INIS)

Apoptosis, or programmed cell death (PCD), is a fundamental process that protects organismal integrity. In earlier work, we demonstrated that over-expression of either of two anti-apoptotic members of the BCL-2 family (BCL-2 or BCL-X L could elevate the frequency of radiation-induced mutations at the autosomal TK1 locus in human TK6 lymphoblasts that express wild-type TP53. Ectopic expression of BCL-X L also elevated the frequencies of double-strand break-induced gene conversion. The purpose of this study is to determine if BCL-2 family proteins promote radiation mutagenesis indirectly through their suppression of PCD, or whether the 'pro-mutagenic' function of these proteins can be separated from their anti-apoptotic function. We developed stable transfectants of TK6 cells that express a mutated form of BCL-X L with a single amino acid substitution in the BH1 domain that is known to interfere with the ability to suppress PCD (BCL-X L gly159ala). We also developed stable transfectants ...

2003-08-17

180

ATP-dependent partitioning of the DNA template into supercoiled domains by Escherichia coli UvrAB  

International Nuclear Information System (INIS)

The helicase action of the Escherichia coli UvrAB complex on a covalently closed circular DNA template was monitored using bacterial DNA topoisomerase I, which specifically removes negative supercoils. In the presence of E. coli DNA topoisomerase I and ATP, the UvrAB complex gradually introduced positive supercoils into the input relaxed plasmid DNA template. Positive supercoils were not produced when E. coli DNA topoisomerase I was replaced by eukaryotic DNA topoisomerase I or when both E. coli and eukaryotic DNA topoisomerases I were added simultaneously. These results suggest that like other DNA helix-tracking processes, the ATP-dependent action of the UvrAM complex on duplex DNA simultaneously generates both positive and negative supercoils, which are not constrained by protein binding but are torsionally strained. The supercoiling activity of UvrAB on UV-damaged DNA was also studied using UV-damaged plasmid DNA and a mutant UvrA protein that lacks the 40 ...

181

[HBsAG in feces, urine and saliva].  

Science.gov (United States)

After some observations about the tests of the research exposed in the literature, authors illustrate the tests for 142 patients divided into 5 groups: a) patients affected with acute viral hepatitis; b) patients affected with praegressa acute viral hepatitis; c) relatives of patients with acute viral hepatitis; d) volunteers; e) patients affected with chronic uraemia under dialisis periodic treatment. After the testing control, authors, conclude with an hipotesis: a possible epidemic function of faeces, urine saliva, in the passage of the acute viral hepatitis. PMID:488577

182

Visually scoring hirsutism  

UK PubMed Central (United Kingdom)

BACKGROUNDHirsutism is the presence of excess body or facial terminal (coarse) hair growth in females in a male-like pattern, affects 5–15% of women, and is an important...Full Text Available

2010-01-01

183

Vehicle-Dependent Disposition Kinetics of Fluoranthene in Fisher-344 Rats  

UK PubMed Central (United Kingdom)

The objective of this study was to evaluate how the vehicles of choice affect the pharmacokinetics of orally administered Fluoranthene [FLA] in rats. Fluoranthene is a member of the family of...Full Text Available

2008-03-01

184

The importance of radiation quality for optimisation in radiology  

UK PubMed Central (United Kingdom)

Selection of the appropriate radiation quality is an important aspect of optimisation for every clinical imaging task in radiology, since it affects both image quality and patient dose. Spreadsheet...Full Text Available

185

The early phase change Gene in Maize  

UK PubMed Central (United Kingdom)

Recessive mutations of the early phase change (epc) gene in maize affect several aspects of plant development. These mutations were identified initially because of...Full Text Available

2002-01-01

186

The Mitoscriptome in Aging and Disease  

UK PubMed Central (United Kingdom)

Mitochondria are the major sites where energy is produced in the cell. Functions of organs such as the heart which has high energy demand are seriously affected by dysfunction of mitochondria....Full Text Available

2011-04-19

187

The Effect of Boron on the Mechanical Behavior of Copper ...  

Science.gov (United States)

... 500 ppm, boron increases the 0.2% offset strength; results show that boron doping does not appreciably affect ultimate compressive strength; for 0 ...

188

Technical Lio - NASA Technical Reports Server  

Science.gov (United States)

Mod IE did not affect the valve flow characteristics. C. BIPROPELLANT -VALVE EVALUATION. 1. Ball and Shaft Seal Evaluation ...

189

Substitution of Glycine 275 by Glutamate (G275E) in Lipase of Bacillus stearothermophilus Affects Its Catalytic Activity and Enantio- and Chain Length Specificity  

KoreaScience (Korea)

Full Text Available

2000-12-01

190

Some new considerations about factors affecting public acceptance of nuclear energy  

International Nuclear Information System (INIS)

(1978). United States Hukai, D. Boston Univ., MA Simmons, RE Hukai, RY

191

Shoulder pain  

UK PubMed Central (United Kingdom)

IntroductionShoulder pain covers a wide range of problems and affects up to 20% of the population. It is not a specific diagnosis. Shoulder pain can be caused by problems with the...Full Text Available

192

Referential Failures and Affective Reactivity of Language in Schizophrenia and Unipolar Depression  

British Library Electronic Table of Contents (United Kingdom)

Reference failures, and their increase in affectively negative conditions (known as affective reactivity of speech), are more frequently observed in schizophrenia patients than in normal controls, but no information is available comparing schizophrenia with depression, ie, a mental disorder closely linked to the concept of affective reactivity. To address this gap in the literature, the present study compared 24 schizophrenia inpatients, 21 unipolar depression inpatients and 21 normal controls. Two 10-minute conversational speech samples (1 on negative and 1 on positive memories) were collected from each patient. The transcripts of the audiotaped interviews were analyzed blindly for frequencies of 6 types of referential failures, employing the Communication Disturbances Index, adapted for ...

2011-01-01

193

Progression of liver cirrhosis to HCC: an application of hidden Markov model  

UK PubMed Central (United Kingdom)

BackgroundHealth service databases of administrative type can be a useful tool for the study of progression of a disease, but the data reported in such sources could be affected...Full Text Available

194

Polymorbidity in diabetes in older people: consequences for care and vocational training  

UK PubMed Central (United Kingdom)

ObjectiveTo investigate the prevalence of complicating and concurrent morbidities in older diabetic patients and to evaluate to what extent their occurrence affects the burden of...Full Text Available

2007-12-01

195

Polymer Genomics: An Insight into Pharmacology and Toxicology of Nanomedicines 1  

UK PubMed Central (United Kingdom)

Synthetic polymers and nanomaterials display selective phenotypic effects in cells and in the body that affect signal transduction mechanisms involved in inflammation, differentiation, proliferation,...Full Text Available

2006-12-30

196

Parental infertility and sexual maturation in children  

UK PubMed Central (United Kingdom)

BACKGROUNDThe reproductive health of children born of infertile couples may be affected by infertility treatment or factors associated with infertility. We examined sexual maturation...Full Text Available

2009-02-01

197

Pacification: The Overall Strategy in South Vietnam  

Science.gov (United States)

... implementation." 4 1 Factors Affecting Rural Construction. Two new factors profoundly modify today's pacification equa- tion. ...

1966-04-22

198

Occupational Asthma: Etiologies and Risk Factors  

UK PubMed Central (United Kingdom)

The purpose of this article is to critically review the available evidence pertaining to occupational, environmental, and individual factors that can affect the development of occupational asthma (OA)....Full Text Available

2011-07-01

199

Nociceptive and Anxiety-Like Behavior in Reproductively Competent and Reproductively Senescent Middle-Aged Rats  

UK PubMed Central (United Kingdom)

BackgroundChanges in levels of estradiol and progesterone that occur with the transition to reproductive senescence may influence nociception or affect.Full Text Available

2009-01-01

200

Neural processing of asynchronous audiovisual speech perception  

UK PubMed Central (United Kingdom)

The temporal synchrony of auditory and visual signals is known to affect the perception of an external event, yet it is unclear what neural mechanisms underlie the influence of temporal synchrony...Full Text Available

2010-02-15

201

Military Base Closures and Affected Defense Department Civil ...  

Science.gov (United States)

... System (CSRS) and the Federal Employees Retirement System (FERS). ... retirement, thrift savings plan, annual and sick leave, severance pay and ...

2006-03-07

202

Metallurgical instabilities during the high temperature low cycle fatigue of nickel-base superalloys  

Energy Technology Data Exchange (ETDEWEB)

In this letter, examples of microstructural instabilities that can significantly affect the low crystal fatigue (LCF) life are pointed out.

1983-01-01

203

Medical problems affecting musicians.  

UK PubMed Central (United Kingdom)

The physical demands of performing on musical instruments can cause pain, sensory loss, and lack of coordination. Five cases illustrate common problems. Knowledge of the interaction between the technique...Full Text Available

1995-12-01

204

Maternal deprivation stress as a risk factor for mental health: Neurotrophins as neuroendocrine signals in rodents and non-human primates  

International Nuclear Information System (INIS)

... for mediating the effects of early adverse experiences since they are affected

2008-10-01

205

Mapping Deforestation and Land Use in Amazon Rainforest Using ...  

Science.gov (United States)

Jan 22, 2011 ... Abstract: Land use changes and deforestation in tropical rainforests are among the major factors affecting the overall function of the global ...

206

Malaria transmission in two localities in north-western Argentina  

UK PubMed Central (United Kingdom)

BackgroundMalaria is one of the most important tropical diseases that affects people globally. The influence of environmental conditions in the patterns of temporal distribution...Full Text Available

207

MODIFIED AMBIENT TEMPERATURE PERCEPTION AFFECTS DAILY ACTIVITY PATTERNS IN THE EUROPEAN GROUND SQUIRREL (SPERMOPHILUS ...  

Science.gov (United States)

... in full sun and in shade, using an infrared thermometer (MiniTemp Noncontact, Conrad Electronic, Nürnberg, Germany). Time intervals ... ...

208

Lichen striatus - close-up (image)  

Science.gov (United States)

Lichen striatus begins similarly to lichen planus, with small bumps (papules), but over a period of days expands to form a long streak or band of affected ...

209

Intelligence Oversight Revisited: Does CONUS Base Security ...  

Science.gov (United States)

... Department of Defense (DoD) Directive 5240.1-R. Procedures Governing the Activities of DoD Intelligence Components that Affect United States ...

2001-04-01

210

Healthy aging and disease: role for telomere biology?  

UK PubMed Central (United Kingdom)

Aging is a biological process that affects most cells, organisms and species. Human aging is associated with increased susceptibility to a variety of chronic diseases, including cardiovascular disease,...Full Text Available

2011-05-01

211

Greenversations » What you can do  

Science.gov (United States)

where I was in El Paso, I could always look up and see the smokestacks of the old Asarco smelter looming. The facility affected the city in more ways than that constant visual...

2011-10-07

212

Greenversations » Water  

Science.gov (United States)

where I was in El Paso, I could always look up and see the smokestacks of the old Asarco smelter looming. The facility affected the city in more ways than that constant visual...

2011-10-07

213

Factors Affecting Attrition Among Marine Corps Women  

Science.gov (United States)

... 2. Help women become accepted by providing job-related training, creating flexible work groups with good climates, and encourage supervisors to ...

1985-12-01

214

Expression and regulation of the NALP3 inflammasome complex in periodontal diseases  

UK PubMed Central (United Kingdom)

Periodontitis is an infectious process characterized by inflammation affecting the supporting structures of the teeth. Porphyromonas gingivalis is a major oral bacterial species implicated...Full Text Available

2009-09-01

215

Evaluation of adhesive bonded sandwich panels for ...  

Science.gov (United States)

... content of the environment) are significant factors affecting the useful service life of adhesive bonded plastic composite sandwich panels at 650 F ...

1970-10-01

216

Effects of material factors on stress corrosion cracking growth in the heat affected zones of austenitic alloy weldments  

International Nuclear Information System (INIS)

... University, Fracture and Reliability Research Institute, Sendai, Miyagi (Japan)

2007-05-01

217

Do Dreissenid Mussels Affect Lake Erie Ecosystem Stability Processes?  

Science.gov (United States)

... associated with non-reporting of phosphorus input from combined sewer overflow discharge all could potentially play a part in ... ...

218

Dendritic Cells in Systemic Lupus Erythematosus  

UK PubMed Central (United Kingdom)

Systemic lupus erythematosus (SLE) persists as a chronic inflammatory autoimmune disease and is characterized by the production of autoantibodies and immune complexes that affects multiple organs....Full Text Available

2010-04-01

219

Consanguinity and fetal growth in Pakistani Moslems.  

UK PubMed Central (United Kingdom)

There is conflicting evidence about the effect of parental consanguinity on fetal growth. Previous studies have not always allowed for other factors that are known to affect birth weight, in particular,...Full Text Available

1987-03-01

220

Congenital Lobar Emphysema  

UK PubMed Central (United Kingdom)

SummaryCongenital lobar emphysema (CLE) characterized by over distension and air-trapping in the affected lobe is one of the causes of infantile respiratory distress requiring...Full Text Available

2009-08-01

221

Coast Guard. Challenges Affecting Deepwater Asset ...  

Science.gov (United States)

... Deepwater Program is a 25-year, $24 billion plan to replace or modernize its fleet of vessels, aircraft, and information management capabilities. ...

2007-06-01

222

Cardiac Angiosarcoma-Associated Membranoproliferative Glomerulonephropathy  

UK PubMed Central (United Kingdom)

Primary cardiac angiosarcoma is a rare cardiac tumor. The initial clinical course is often asymptomatic, and metastatic disease is present in a majority of affected patients at diagnosis. We present...Full Text Available

2011-01-01

223

Application of chloropicrin to Douglas-fir stumps to control laminated ...  

Science.gov (United States)

Sep 1, 2011 ... Title: Application of chloropicrin to Douglas-fir stumps to control laminated root rot does not affect infection or growth of regeneration 16 growing ...

224

Antihypertensive Agents in Hemodialysis Patients: A Current Perspective  

UK PubMed Central (United Kingdom)

Hypertension affects most hemodialysis patients and is often poorly controlled. Adequate control of blood pressure is difficult with conventional hemodialysis alone but is important to improve...Full Text Available

2010-05-01

225

Airborne minerals and related aerosol particles: Effects on climate and the environment  

UK PubMed Central (United Kingdom)

Aerosol particles are ubiquitous in the troposphere and exert an important influence on global climate and the environment. They affect climate through scattering, transmission, and absorption of...Full Text Available

1999-03-30

226

Aeroelastic Analysis by Coupled Non-linear Time Domain ... - NASA  

Science.gov (United States)

and shock induced flow separation may significantly affect the flutter ...... Aerodynamic and Aeroelastic Simulation, AGARD Report 822, 10-1 10-20. ...

227

Phosphomimetic mutation of the mitotically phosphorylated serine 1880 compromises the interaction of the transmembrane nucleoporin gp210 with the nuclear pore complex  

International Nuclear Information System (INIS)

The nuclear pore complexes (NPCs) reversibly disassemble and reassemble during mitosis. Disassembly of the NPC is accompanied by phosphorylation of many nucleoporins although the function of this is not clear. It was previously shown that in the transmembrane nucleoporin gp210 a single serine residue at position 1880 is specifically phosphorylated during mitosis. Using amino acid substitution combined with live cell imaging, time-lapse microscopy and FRAP, we investigated the role of serine 1880 in binding of gp210 to the NPC in vivo. An alanine substitution mutant (S1880A) was significantly more dynamic at the NPC compared to the wild-type protein, suggesting that serine 1880 is important for binding of gp210 to the NPC. Moreover a glutamate substitution (S1880E) closely mimicking phosphorylated serine specifically interfered with incorporation of gp210 into the NPC and compromised its post-mitotic recruitment to the nuclear envelope of daughter nuclei. Our ...

2007-07-15

228

Microaerophilic growth and induction of the photosynthetic reaction center in Rhodopseudomonas viridis  

Energy Technology Data Exchange (ETDEWEB)

Rhodopseudomonas viridis was grown in liquid culture at 30 degrees C anaerobically in light (generation time, 13 h) and under microaerophilic growth conditions in the dark (generation time, 24 h). The bacterium could be cloned at the same temperature anaerobically in light (1 week) and aerobically in the dark (3 to 4 weeks) if oxygen was limited to 0.1%. Oxygen could not be replaced by dimethyl sulfoxide, potassium nitrate, or sodium nitrite as a terminal electron acceptor. No growth was observed anaerobically in darkness or in the light when air was present. A variety of additional carbon sources were used to supplement the standard succinate medium, but enhanced stationary-phase cell density was observed only with glucose. Conditions for induction of the photosynthetic reaction center upon the change from microaerophilic to phototrophic growth conditions were investigated and optimized for a mutant functionally defective in phototrophic growth. R. viridis ...

1989-05-01

229

Maintenance and regulation of extracellular volume and the ion environment in Drosophila larval nerves.  

Science.gov (United States)

In mammals and insects, paracellular blood barriers isolate the nervous system from the rest of the animal. Glia and accessory cells of the nervous system use pumps, channels, cotransporters, and exchangers collectively to maintain the extracellular ion environment and osmotic balance in the nervous system. At present, the molecular mechanisms that regulate this process remain unclear. In humans, loss of extracellular ion and volume regulation in the nervous system poses serious health threats. Drosophila is a model genetic organism with a proven track record for uncovering molecular mechanisms relevant to human health and disease. Here, we review what is known about extracellular ion and volume regulation in larval abdominal nerves, present some new data about the impact of neural activity on the extracellular environment, and relate the findings to mammalian systems. Homologies have been found at the level of morphology, physiology, molecular mechanisms, and ...

2011-02-08

230

Identification of the binding domain for NADP"+ of human glucose-6-phosphate dehydrogenase by sequence analysis of mutants  

International Nuclear Information System (INIS)

Human erythrocyte glucose-6-phosphate is normally quite stable in the presence of 10 #mu#M NADP"+. Certain glucose-6-phosphate dehydrogenase variants lose virtually all their activity at this concentration of NADP"+ but are reactivated by 200 #mu#M NADP"+. Such variants presumably have a defect in their NADP"+-binding site. The authors analyzed the sequence of cDNA or genomic DNA from seven unrelated patients with hemolytic anemia due to the inheritance of variants that are reactivated by NADP"+. Six patients had substitutions of one of three adjacent amino acids, and the seventh patient had another amino acid substitution 23 residues downstream. These amino acids are highly conserved, all being present in rat and all but one being found also in Drosophila. The anomalous electrophoretic behavior of some of the variants can be explained by their loss of ability to bind NADP"+. The conclude that the region in which these mutations occur defines the binding domain for NADP"+ and that ...

231

Identification of protein phosphorylation sites within Ser/Thr-rich cluster domains using site-directed mutagenesis and hybrid linear quadrupole ion trap Fourier transform ion cyclotron resonance mass spectrometry  

British Library Electronic Table of Contents (United Kingdom)

We describe a method for the analysis of multi-site phosphorylation in serine/threonine (Ser/Thr)-rich protein sequences. Site-specific mutagenesis was used to introduce tryptic cleavage sites in the serine glutamine/threonine glutamine cluster domain (SCD) of the human checkpoint protein kinase (Chk2). The mutant proteins were shown to autophosphorylate on residues that are inducibly phosphorylated when mammalian cells are exposed to ionizing radiation (serine 33/35, serine 516, threonine 68 and threonine 432). Five Ser/Thr clusters within the SCD were flanked by arginine or lysine residues to produce tryptic peptides for nanospray liquid chromatography (nanoLC)/linear quadrupole ion trap Fourier transform ion cyclotron resonance mass spectrometry. Phosphorylation sites were assigned usin...

2007-01-01

232

Host derived inflammatory phospholipids regulate rahU (PA0122) Gene, Protein, and Biofilm Formation in Pseudomonas aeruginosa  

British Library Electronic Table of Contents (United Kingdom)

This study describes the role of "inflammatory" oxidized (Ox) phospholipids in regulation of rahU (PA0122) expression and biofilm formation in Pseudomonas aeruginosa (383) wild type (rahU+) and rahU mutant (rahU-) strains. Functional analysis of RahU protein from P. aeruginosa in presence of Ox-phospholipids show: (a) LysoPC modulates RahU gene/and protein expression in rahU+ cells; (b) rahU promoter activity is increased by lysoPC and inhibited by PAPC, Ox-PAPC and arachidonic acid; the latter inhibitory effect can be reversed by lysoPC, which was enzymatically derived from PAPC; (c) biofilm formation increased in rahU- cells as compared to rahU+; and (d) inhibition of rahU promoter activity by PAPC and AA (but not lysoPC) showed significantly augmented biofilm formation in rahU+ but not ...

2011-01-01

233

High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal  

British Library Electronic Table of Contents (United Kingdom)

Promising approaches to produce higher alcohols, e.g., isobutanol, using Escherichia coli have been developed with successful results. Here, we translated the isobutanol process from shake flasks to a 1-L bioreactor in order to characterize three E. coli strains. With in situ isobutanol removal from the bioreactor using gas stripping, the engineered E. coli strain (JCL260) produced more than 50?g/L in 72?h. In addition, the isobutanol production by the parental strain (JCL16) and the high isobutanol-tolerant mutant (SA481) were compared with JCL260. Interestingly, we found that the isobutanol-tolerant strain in fact produced worse than either JCL16 or JCL260. This result suggests that in situ product removal can properly overcome isobutanol toxicity in E. coli cultures. The isobutanol prod...

2011-01-01

234

Genetical approach to oxygen toxicity. [Saccharomyces cerevisiae  

Energy Technology Data Exchange (ETDEWEB)

The role of intermediate products of dioxygen reduction in cytotoxic effects ascribed to oxygen molecules was studied in vivo using various yeast mutants with changed response to oxygen stress. It has been documented that superoxide radical exerts its deleterious effects on yeast cells directly and the role of other oxygen species derived from it is hardly detectable. Yeast Saccharomyces cerevisiae, however, cannot be considered as a typical eukaryotic organism due to its inability of synthesizing polyunsaturated fatty acids (pufa). These fatty acids are known as main target molecules during oxidative stress and their peroxidation leads to cytotoxic effects. As fatty acid content could be easily manipulated in yeast, this organism was used to evaluate the contribution of pufa peroxidation process to the cytotoxic effects of oxygen. Results obtained show, that yeast cells containing linolenic acid as the main constituent of phospholipids are hypersensitive to oxygen ...

1986-01-01

235

Functional expression of anti-hepatitis B virus (HBV) preS2 antigen scFv by cspA promoter system in Escherichia coli and application as a recognition molecule for single-walled carbon nanotube (SWNT) field effect transistor (FET)  

British Library Electronic Table of Contents (United Kingdom)

The preS2 antigens of hepatitis B virus (HBV), which causes a serious health problem in the world, have been implicated in hepatocyte cell binding and viral penetration. Therefore, the importance of antibody production against preS2 antigen for early diagnosis of HBV has been well established. In this study, the recombinant HBV preS2 single chain variable fragment (scFv) antibody was successfully expressed in E. coli with the novel cold shock vector (pCold) under the cspA promoter, and its expression level was compared with the pET vector under the T7 promoter. Additionally, a host with an oxidizing cytoplasm, E. coli trxB/gor double mutant, was used to improve the soluble expression. The anti-HBV preS2 scFv using pCold vector was successfully expressed in a soluble and functional form in ...

2010-01-01

236

Final report of the specific research. Investigations on the analysis of bio-protective factors against radiation. 1998-2000 FY (Research Group of NIRS)  

Energy Technology Data Exchange (ETDEWEB)

This report concerns investigations in the title conducted by 8 groups of National Institute of Radiological Sciences (NIRS) during the period of 1998-2000. The groups are for investigation of: Effects of p53 tumor suppressor gene in radiation-induced leukemia, Role of atm-gene in dose rate effect of ionizing radiation, Function of DNA-dependent protein kinase catalytic subunit (DNA-PK{sub cs}), Functional complementation of radiation-sensitive mutant M10 cell line by human XRCC4 cDNA expression, Role of radiation-induced apoptosis in digital defects in embryonic mice, Functional analysis of S-phase specific novel nuclear protein NP95 by gene targeting, Role of chemokine in T cell development and lymphomagenesis, and establishment of production techniques of gene-modified mice using embryonic stem cells for genetic analysis of radiation-sensitive genes. The groups describe summaries of their studies and published original articles are also given. (N.I.)

2002-03-01

237

Comparative action spectrum for ultraviolet light killing of mouse melanocytes from different genetic coat color backgrounds  

International Nuclear Information System (INIS)

The photobiology of mouse melanocyte lines with different pigment genotypes was studied by measuring colony-forming ability after irradiation. The cell lines were wild-type black (melan-a) and the mutants brown (melan-b) and albino (melan-c). Four lamps emitting various UV wavelengths were used. These were germicidal (UVC, 200-280 nm), 82.3% output at 254 nm, TL01 (UVB, 280-320 nm), 64.2% at 310-311 nm, FS20, broadband with peak output at 312 nm and Alisun-S (UVA, 320-400 nm), broadband with peak output at 350-354 nm. Appropriate filtration reduced the contaminating UVC to nonlethal levels for the longer waverange lamps. It is clear from these studies that, in pigment cells, monochromatic results cannot predict polychromatic responses and that cell death from solar irradiations is a complex phenomenon that depends on more than DNA damage. (author).

1997-01-01

238

Boron-Dependent Degradation of NIP5;1 mRNA for Acclimation to Excess Boron Conditions in Arabidopsis.  

Science.gov (United States)

Boron (B) is an essential plant micronutrient that is toxic at higher levels. NIP5;1 is a boric acid channel required for B uptake and growth under B deficiency. Accumulation of the NIP5;1 transcript is upregulated under B deficiency in Arabidopsis thaliana roots. To elucidate the mechanism of regulation, the 5' untranslated region (UTR) of NIP5;1 was tested for its ability to confer B-dependent regulation using ?-glucuronidase and green fluorescent protein as reporters. This analysis showed that the 5' UTR was involved in NIP5;1 transcript accumulation in response to B conditions. We also found that high-B conditions trigger NIP5;1 mRNA degradation and that the sequence from +182 to +200 bp in the 5' UTR is required for this mRNA destabilization. In the nip5;1-1 mutant background, a NIP5;1 complementation construct without the 5' UTR produced high levels of mRNA accumulation, increased B concentrations in tissues, and reduced growth under high-B conditions. These ...

2011-09-01

239

Bacterial Pili exploit integrin machinery to promote immune activation and efficient blood-brain barrier penetration.  

Science.gov (United States)

Group B Streptococcus (GBS) is the leading cause of meningitis in newborn infants. Bacterial cell surface appendages, known as pili, have been recently described in streptococcal pathogens, including GBS. The pilus tip adhesin, PilA, contributes to GBS adherence to blood-brain barrier (BBB) endothelium; however, the host receptor and the contribution of PilA in central nervous system (CNS) disease pathogenesis are unknown. Here we show that PilA binds collagen, which promotes GBS interaction with the ?(2)?(1) integrin resulting in activation of host chemokine expression and neutrophil recruitment during infection. Mice infected with the PilA-deficient mutant exhibit delayed mortality, a decrease in neutrophil infiltration and bacterial CNS dissemination. We find that PilA-mediated virulence is dependent on neutrophil influx as neutrophil depletion results in a decrease in BBB permeability and GBS-BBB penetration. Our results suggest that the bacterial pilus, ...

2011-09-06

240

A single amino acid substitution results in a retinoblastoma protein defective in phosphorylation and oncoprotein binding  

International Nuclear Information System (INIS)

The authors have previously identified a small-cell lung cancer cell line (NCI-H209) that expresses an aberrant, underphosphorylated form of the retinoblastoma protein RB1. Molecular analysis of RB1 mRNA from this cell line revealed a single point mutation within exon 21 that resulted in a nonconservative amino acid substitution (cysteine to phenylalanine) at codon 706. Stable expression of this mutant RB1 cDNA in a human cell line lacking endogenous RB1 demonstrated that this amino acid change was sufficient to inhibit phosphorylation. In addition, this cysteine-to-phenylalanine substitution also resulted in loss of RB1 binding to the simian virus 40 large tumor and adenovirus E1A transforming proteins. These results confirm the importance of exon 21 coding sequences and suggest that the cysteine residue at codon 706 may play a role in achieving a specific protein conformation essential for protein-protein interactions.

241

( sup 14 C)-Sucrose uptake by guard cell protoplasts of pisum sativum, argenteum mutant  

Energy Technology Data Exchange (ETDEWEB)

Guard cells rely on import for their supply with reduced carbon. The authors tested by silicone oil centrifugation the ability of guard cell protoplasts to accumulated ({sup 14}C)-sucrose. Uptake rates were corrected after measurement of {sup 14}C-sorbitol and {sup 3}H{sub 2}O spaces. Sucrose uptake followed biphasic kinetics, with a high-affinity component below 1 mM external sucrose (apparent K{sub m} 0.8 mM at 25C) and a low-affinity nonsaturable component above. Uptake depended on pH (optimum at pH 5.0). Variations in the concentrations of external KCl, CCCP, and valinomycin indicated that about one-half of the sucrose uptake rate could be related to an electrochemical gradient across the plasmalemma. Total uptake rates measured at 5 mM external sucrose seem to be sufficient to replenish emptied plastids with starch within a few hours.

1991-05-01

242

Regulation of the pT181 encoded tetracycline resistance gene in Straphylococcus aureus  

Energy Technology Data Exchange (ETDEWEB)

pT181 is a naturally-occurring 4437 basepair (bp) plasmid isolated from Staphylococcus aureus which encodes inducible resistance to tetracycline (Tc). The DNA sequence data has identified three open reading frames (ORFs). The largest ORF B, has been found to be responsible for the Tc resistance phenotype of pT181. Since most Tc resistance systems appear to be regulated by an effector protein and a repressor protein, several Bal 31 deletion mutants of pT181 were constructed and analyzed in an effort to identify the elements involved in Tc resistance. Two transcomplementing groups of mutants were identified within the tet gene. The mechanism of Tc resistance was studied by assaying the accumulation of (7-/sup 3/H) Tc by Tc sensitive cells, and uninduced and induced pT181-containing cells. A sharp decrease in accumulation of the drug after an initial increase was observed in Tc induced pT181-containing cells. In vivo labeling of Bacillus subtilis ...

1986-01-01

243

NAME=\\  

Wastenet

... All rickettsial diseases respond to treatment with antibiotics such as doxycycline and tetracycline As of 10 May, the Government of South Africa has reported 186 confirmed cases of RVF in humans, including 18 deaths, in Free State Province, Eastern Cape Province, Northern Cape Province, Western Cape, and North West Province. RVF is a viral disease that primarily affects animals (such as cattle, buffalo, sheep, goats and camels). The disease can also affect humans. The main mode of transmission of RVF ...

244

First results of preoperative localisation of parathyroid glands adenomas by means of subtraction scintigraphy with technetium-99m and thallium-201  

International Nuclear Information System (INIS)

Eight patients with primary hyperparathyroidism were examined preoperative to locate the affected parathyroid glands. The localisation was done with technetium 99m and thallium 201. This new method seems to be of great value to locate preoperative the affected parathyroid glands. (R.B.).

245

Factors affecting costs and utilization of type 2 diabetes healthcare: a cross-sectional survey among 15 hospitals in urban China  

UK PubMed Central (United Kingdom)

BackgroundType 2 Diabetes mellitus (T2DM) affects persons of all ages, while also placing heavy economic burdens on national economies and healthcare systems. The study aims to investigate...Full Text Available

246

Density of states of ordered and disordered A-15 phase  

International Nuclear Information System (INIS)

Within the tight binding framework, a study is made of how the disorder affects the electronic properties of A-15 compounds. In particular it is shown that for compounds of two transition metals in the A-15 structure, the vacancy formation affects the density of states only in the low energy region or in the high energy region, in opposition with the isolated chain model of Labbe and Friedel. It is concluded that interchain interactions are important. (U.K.).

247

Psychological ownership and affective reaction: Emotional attachment process variables and the endowment effect  

British Library Electronic Table of Contents (United Kingdom)

This research proposes that the concept of emotional attachment, and specifically the independent constructs of psychological ownership and affective reaction, can help explain many of the endowment effect findings documented in the literature. We define these constructs and then test them across a set of nine studies in which we both replicate previous and generate new endowment effect findings, and then show that psychological ownership and affective reaction can mediate the effects. In doing so, we offer direct empirical support for the idea of emotional attachment as a driver of loss aversion while also providing practitioners and future endowment effect researchers with new insights about the psychological processes that underlie the endowment effect.

2011-01-01

248

Growth and gas exchange response to water shortage of a maize crop on different soil types  

British Library Electronic Table of Contents (United Kingdom)

The effect of water shortage on growth and gas exchange of maize grown on sandy soil (SS) and clay soil was studied. The lower soil water content in the SS during vegetative growth stages did not affect plant height, above-ground biomass, and leaf area index (LAI). LAI reduction was observed on the SS during the reproductive stage due to early leaf senescence. Canopy and leaf gas exchanges, measured by eddy correlation technique and by a portable photosynthetic system, respectively, were affected by water stress and a greater reduction in net photosynthetic rate (A N) and stomatal conductance (g s) was observed on SS. Chlorophyll and carotenoids content was not affected by water shortage in either condition. Results support two main conclusions: (1) leaf photosynthetic capacity was unaffec...

2009-01-01

249

ENSO affects sex ratio progeny in captive Iberian red deer despite a steady feeding regime  

British Library Electronic Table of Contents (United Kingdom)

Climate variability greatly affects animals through direct and indirect effects. Animals with slow reproductive adaptation to ecological changes such as large mammals are likely to have evolved mechanisms to anticipate early such impacts of climate variability on the environment. One of the adaptive mechanisms between reproductive costs and benefits in mammals affects parental investment through biases in sex ratio. Deer might be likely to show an early detection of climate variability because conception takes place in early autumn, but the main raising cost in deer concerns lactation, which takes place at the end of the following spring. The aim of this paper is to assess whether there is a relationship between global indices of climate variability such as El Ni?o-Southern Oscillation (EN...

2011-01-01

250

Visual Genome-Wide RNAi Screening to Identify Human Host Factors Required for Trypanosoma cruzi Infection  

UK PubMed Central (United Kingdom)

The protozoan parasite Trypanosoma cruzi is the etiologic agent of Chagas disease, a neglected tropical infection that affects millions of people in the Americas. Current chemotherapy...Full Text Available

251

Viewpoint: Policy Requirements for Protecting Wildlife from Endocrine Disruptors  

UK PubMed Central (United Kingdom)

Man-made endocrine-disrupting chemicals (EDCs) present a threat to biodiversity, even in remote areas. To date, numerous wildlife species have been affected by EDCs in the environment, but it is likely...Full Text Available

2006-04-01

252

Untreated Poor Vision: A Contributing Factor to Late-Life Dementia  

UK PubMed Central (United Kingdom)

Ophthalmologic abnormalities have been described in patients with dementia, but the extent to which poor vision and treatment for visual disorders affect cognitive decline is not well defined. Linked...Full Text Available

2010-03-15

253

Ultrasound-Guided Three-In-One Nerve Block for Femur Fractures  

UK PubMed Central (United Kingdom)

Femur fractures typically affect elderly patients with multiple co-morbidities. Pain control can be difficult, requiring intensive nursing and physician care as elderly patients may manifest cardiovascular...Full Text Available

2010-09-01

254

Tinea nigra by Hortaea werneckii, a report of 22 cases from Mexico  

UK PubMed Central (United Kingdom)

Tinea nigra is a superficial mycosis caused by Hortaea werneckii. It is an infrequent asymptomatic infection that affects human palms and soles, and is mostly observed in tropical...Full Text Available

2008-01-01

255

The state of public power  

Energy Technology Data Exchange (ETDEWEB)

Opinions on factors affecting public power systems are presented. Social, economic, and political factors are discussed in terms of their impact on public power. Competition with investor-owned utilities is the primary focus of the paper. Privatization and regulations are other issues discussed.

1995-12-31

256

The safety and efficacy of tolterodine extended release in the treatment of overactive bladder in the elderly  

UK PubMed Central (United Kingdom)

After lifestyle and behavioral measures to control overactive bladder, the mainstay of pharmacological treatment is the use of antimuscarinic therapy. Overactive bladder predominantly affects older...Full Text Available

2009-01-01

257

The role of DNA damage response pathways in chromosome fragility in Fragile X syndrome  

UK PubMed Central (United Kingdom)

FRAXA is one of a number of fragile sites in human chromosomes that are induced by agents like fluorodeoxyuridine (FdU) that affect intracellular thymidylate levels. FRAXA coincides with a >200...Full Text Available

2009-07-01

258

The plant oncogene rolC is responsible for the release of cytokinins from glucoside conjugates.  

UK PubMed Central (United Kingdom)

The rolC gene of Agrobacterium rhizogenes, which drastically affects growth and development of transgenic plants, codes for a cytokinin-beta-glucosidase. Indeed, rolC protein expressed in Escherichia...Full Text Available

1991-10-01

259

The luxS Gene of Streptococcus pyogenes Regulates Expression of Genes That Affect Internalization by Epithelial Cells  

UK PubMed Central (United Kingdom)

The gram-positive pathogen Streptococcus pyogenes was recently reported to possess a homologue of the luxS gene that is responsible for the production of autoinducer...Full Text Available

2003-10-01

260

The high Andes, gene flow and a stable hybrid zone shape the genetic structure of a wide-ranging South American parrot  

UK PubMed Central (United Kingdom)

BackgroundWhile the gene flow in some organisms is strongly affected by physical barriers and geographical distance, other highly mobile species are able to overcome such constraints....Full Text Available

261

The future of dentistry: new challenges, new directions.  

UK PubMed Central (United Kingdom)

The challenge to our profession today is to improve the quality of oral health while overcoming both extrinsic and intrinsic factors which may adversely affect our progress toward this goal. The combined...Full Text Available

1990-05-01

262

The erratic mitochondrial clock: variations of mutation rate, not population size, affect mtDNA diversity across birds and mammals  

UK PubMed Central (United Kingdom)

BackgroundDuring the last ten years, major advances have been made in characterizing and understanding the evolution of mitochondrial DNA, the most popular marker of molecular biodiversity....Full Text Available

263

The effect of IL6-174C/G polymorphism on postprandial triglyceride metabolism in the GOLDN study*  

UK PubMed Central (United Kingdom)

Chronically elevated interleukin-6 (IL-6) affects lipid and lipoprotein metabolism. Individuals genetically predisposed to higher IL-6 secretion may be at risk of dyslipidemia, especially during the...Full Text Available

2008-08-01

264

The attitudes of primary schoolchildren in Northern Thailand towards their peers who are affected by HIV and AIDS  

UK PubMed Central (United Kingdom)

After more than a decade of the AIDS epidemic in Thailand, the number of children whose parents are living with HIV or have died from AIDS is increasing significantly and it has been reported that these...Full Text Available

2011-02-09

265

The Role of Thyroid Hormone in Testicular Development and Function  

UK PubMed Central (United Kingdom)

Thyroid hormone is a critical regulator of growth, development and metabolism in virtually all tissues, and altered thyroid status affects many organs and systems. Although for many years testis...Full Text Available

2008-12-01

266

Temporal changes in key maternal and fetal factors affecting birth outcomes: A 32-year population-based study in an industrial city  

UK PubMed Central (United Kingdom)

BackgroundThe link between maternal factors and birth outcomes is well established. Substantial changes in society and medical care over time have influenced women's reproductive...Full Text Available

267

Surgery for hip fractures: Does surgical delay affect outcomes?  

UK PubMed Central (United Kingdom)

Hip fractures are associated with a high rate of mortality and profound temporary and sometimes permanent impairment of quality of life. Current guidelines indicate that surgeons should perform surgery...Full Text Available

2011-01-01

268

Succession of Indigenous Pseudomonas spp. and Actinomycetes on Barley Roots Affected by the Antagonistic Strain Pseudomonas fluorescens DR54 and the Fungicide Imazalil  

UK PubMed Central (United Kingdom)

In recent years, the interest in the use of bacteria for biological control of plant-pathogenic fungi has increased. We studied the possible side effects of coating barley seeds with the antagonistic...Full Text Available

2001-03-01

269

Submergence-Induced Morphological, Anatomical, and Biochemical Responses in a Terrestrial Species Affect Gas Diffusion Resistance and Photosynthetic Performance  

UK PubMed Central (United Kingdom)

Gas exchange between the plant and the environment is severely hampered when plants are submerged, leading to oxygen and energy deficits. A straightforward way to reduce these shortages of oxygen and...Full Text Available

2005-09-01

270

Steroid hormones and brain development: some guidelines for understanding actions of pseudohormones and other toxic agents.  

UK PubMed Central (United Kingdom)

Gonadal, adrenal, and thyroid hormones affect the brain directly, and the sensitivity to hormones begins in embryonic life with the appearance of hormone receptor sites in discrete populations of neurons....Full Text Available

1987-10-01

271

Sperm investment in male meadow voles is affected by the condition of the nearby male conspecifics  

UK PubMed Central (United Kingdom)

Sperm competition occurs when 2 or more males copulate with a particular female during the same reproductive cycle, and their sperm compete to fertilize the female's available eggs. One strategy that...Full Text Available

2008-11-01

272

Spatial analysis of BSE cases in the Netherlands  

UK PubMed Central (United Kingdom)

BackgroundIn many of the European countries affected by Bovine Spongiform Encephalopathy (BSE), case clustering patterns have been observed. Most of these patterns have been interpreted...Full Text Available

273

Sparganosis in the Lumbar Spine : Report of Two Cases and Review of the Literature  

UK PubMed Central (United Kingdom)

Sparganosis is a rare parasitic infection affecting various organs, including the central nervous system, especially the lumbar epidural space. This report describes the identification of disease and...Full Text Available

2011-04-01

274

Social competition affects electric signal plasticity and steroid levels in the gymnotiform fish Brachyhypopomus gauderio  

UK PubMed Central (United Kingdom)

Sexually-selected communication signals can be used by competing males to settle contests without incurring the costs of fighting. Steroid regulation of these signals can render them as reliable...Full Text Available

2009-10-01

275

Short Hypoxia Does not Affect Plasma Leptin in Healthy Men under Euglycemic Clamp Conditions  

UK PubMed Central (United Kingdom)

Leptin is involved in the endocrine control of energy expenditure and body weight regulation. Previous studies emphasize a relationship between hypoxic states and leptin concentrations. The aim of this...Full Text Available

2009-01-01

276

Selection of Inhibitor-Resistant Viral Potassium Channels Identifies a Selectivity Filter Site that Affects Barium and Amantadine Block  

UK PubMed Central (United Kingdom)

BackgroundUnderstanding the interactions between ion channels and blockers remains an important goal that has implications for delineating the basic mechanisms of ion channel function...Full Text Available

277

Safety culture development at Daya Bay NPP  

International Nuclear Information System (INIS)

From view on Organization Behavior theory, the concept, development and affecting factors of safety culture are introduced. The focuses are on the establishment, development and management practice for safety culture at Daya Bay NPP. A strong safety culture, also demonstrated, has contributed greatly to improving performance at Daya Bay

2001-12-01

278

Rural Education and the Beginning Teacher.  

Science.gov (United States)

This paper addresses the major issues beginning teachers face in their first postings to rural areas in Australia. The issues go beyond pedagogy. Beginning teachers need to understand social factors affecting rural communities so they can link lessons to the realities experienced by students. Geographic isolation affects the provision of education in terms of time taken to travel, cost, terrain, and technology. Rural communities have groups that are socially isolated from each other and from the staff in schools. In order to teach effectively, teachers need to be aware of the social context of the community in which they live and work. Teachers in rural areas suffer from professional isolation and often confront teaching situations for which they have had little formal preparation. Communities in remote places often move quickly to adopt technological means to overcome their isolation. Teachers in rural areas need to identify and promote the ...

1992-02-01

279

Root Suberin Forms an Extracellular Barrier That Affects Water Relations and Mineral Nutrition in Arabidopsis  

UK PubMed Central (United Kingdom)

Though central to our understanding of how roots perform their vital function of scavenging water and solutes from the soil, no direct genetic evidence currently exists to support the foundational model...Full Text Available

2009-05-01

280

Role of the nac gene product in the nitrogen regulation of some NTR-regulated operons of Klebsiella aerogenes.  

UK PubMed Central (United Kingdom)

A positive, genetic selection against the activity of the nitrogen regulatory (NTR) system was used to isolate insertion mutations affecting nitrogen regulation in Klebsiella aerogenes. Two classes...Full Text Available

1990-12-01

281

Risperidone in the treatment of bipolar mania  

UK PubMed Central (United Kingdom)

Atypical antipsychotic medications have assumed growing importance for the treatment of bipolar disorder, an illness that affects approximately 1.2%–3.7% of the general population in a given...Full Text Available

2006-06-01

282

Review: Interventional radiology in peripheral vascular disease  

UK PubMed Central (United Kingdom)

Peripheral vascular diseases (PVD) are referred to as diseases affecting the blood vessels other than the heart and the brain. Interventional endovascular treatment whenever feasible has become the...Full Text Available

2008-05-01

283

Rate of Dehydration and Cumulative Desiccation Stress Interacted to Modulate Desiccation Tolerance of Recalcitrant Cocoa and Ginkgo Embryonic Tissues1  

UK PubMed Central (United Kingdom)

Rate of dehydration greatly affects desiccation tolerance of recalcitrant seeds. This effect is presumably related to two different stress vectors: direct mechanical or physical stress because of the...Full Text Available

2002-04-01

284

Putative Biomarkers and Targets of Estrogen Receptor Negative Human Breast Cancer  

UK PubMed Central (United Kingdom)

Breast cancer is a progressive and potentially fatal disease that affects women of all ages. Like all progressive diseases, early and reliable diagnosis is the key for successful treatment and annihilation....Full Text Available

285

Prevalence and determinants of pain and pain-related disability in urban and rural settings in southeastern Ontario  

UK PubMed Central (United Kingdom)

BACKGROUND:Canadian chronic pain prevalence estimates range from 11% to 66%, are affected by sampling and measurement bias, and largely represent urban settings.OBJECTIVES:To...Full Text Available

2006-01-01

286

Polymorphic Regions Affecting Human Height Also Control Stature in Cattle  

UK PubMed Central (United Kingdom)

Orthologous positions of 55 genes associated with height in four human populations were located on the bovine genome. Single nucleotide polymorphisms close to eight of these genes were significantly...Full Text Available

2011-03-01

287

Polyandry increases offspring viability and mother productivity but does not decrease mother survival in Drosophila pseudoobscura  

UK PubMed Central (United Kingdom)

Polyandrous mating is common, but the benefits for females of polyandry remain controversial. To test whether mating with multiple males affects female fitness, we compared lifetime components of fitness...Full Text Available

2010-08-03

288

Phytochrome Control of Another Phytochrome-mediated Process 1  

UK PubMed Central (United Kingdom)

The phytochrome-mediated attachment of root tips of mung bean (Phaseolus aureus) and barley (Hordeum vulgare) to glass is affected by the prior exposure of hydrated...Full Text Available

1972-04-01

289

Phosphorylation-dependent power output of transgenic flies: an integrated study.  

UK PubMed Central (United Kingdom)

We examine how the structure and function of indirect flight muscle (IFM) and the entire flight system of Drosophila melanogaster are affected by phosphorylation of the myosin regulatory light chain...Full Text Available

1997-12-01

290

Personalized Epigenomic Signatures That Are Stable Over Time and Covary with Body Mass Index  

UK PubMed Central (United Kingdom)

The epigenome consists of non–sequence-based modifications, such as DNA methylation, that are heritable during cell division and that may affect normal phenotypes and predisposition...Full Text Available

2010-09-15

291

Perceived Interpersonal Mistreatment Among Obese Americans: Do Race, Class, and Gender Matter?  

UK PubMed Central (United Kingdom)

Objective:We examine the extent to which body weight affects three types of perceived interpersonal mistreatment, and evaluate whether these patterns vary by race,...Full Text Available

2008-11-01

292

Pelvic floor disorders and quality of life in women with self-reported irritable bowel syndrome  

UK PubMed Central (United Kingdom)

SummaryBackgroundQuality of life among women with irritable bowel syndrome may be affected by pelvic floor disorders.AimFull Text Available

2010-02-01

293

Paracetamol reduces influenza-induced immunopathology in a mouse model of infection without compromising virus clearance or the generation of protective immunity  

UK PubMed Central (United Kingdom)

BackgroundSeasonal influenza A infection affects a significant cohort of the global population annually, resulting in considerable morbidity and mortality. Therapeutic strategies...Full Text Available

2011-05-01

294

PHARMACOLOGICAL TREATMENTS FOR TINNITUS: NEW AND OLD  

UK PubMed Central (United Kingdom)

Subjective tinnitus, the phantom ringing or buzzing sensation that occurs in the absence of sound, affects 12–14% of adults; in some cases the tinnitus is so severe or disabling that...Full Text Available

2009-01-01

295

Outbreak of acute colitis on a horse farm associated with tetracycline-contaminated sweet feed.  

UK PubMed Central (United Kingdom)

Exposure of a group of horses to tetracycline-contaminated feed resulted in acute colitis and subsequent death in one horse and milder diarrhea in 3 others. The most severely affected animal demonstrated...Full Text Available

1999-10-01

296

Organ-Specific Invertase Deficiency in the Primary Root of an Inbred Maize Line 1  

UK PubMed Central (United Kingdom)

An organ-specific invertase deficiency affecting only the primary root system is described in the Oh 43 inbred maize (Zea mays). Invertases (acid and neutral/soluble and insoluble)...Full Text Available

1991-10-01

297

Nuclear Receptor Rev-erb Alpha (Nr1d1) Functions in Concert with Nr2e3 to Regulate Transcriptional Networks in the Retina  

UK PubMed Central (United Kingdom)

The majority of diseases in the retina are caused by genetic mutations affecting the development and function of photoreceptor cells. The transcriptional networks directing these processes are regulated...Full Text Available

298

New common variants affecting susceptibility to basal cell carcinoma  

UK PubMed Central (United Kingdom)

In a follow-up to our previously reported genome-wide association study of cutaneous basal cell carcinoma (BCC)1, we describe here several new susceptibility...Full Text Available

2009-08-01

299

Mutations affecting the development of the peripheral nervous system in Drosophila: a molecular screen for novel proteins.  

UK PubMed Central (United Kingdom)

In our quest for novel genes required for the development of the embryonic peripheral nervous system (PNS), we have performed three genetic screens using MAb 22C10 as a marker of terminally differentiated...Full Text Available

2000-12-01

300

Mutation analysis of 272 Spanish families affected by autosomal recessive retinitis pigmentosa using a genotyping microarray  

UK PubMed Central (United Kingdom)

PurposeRetinitis pigmentosa (RP) is a genetically heterogeneous disorder characterized by progressive loss of vision. The aim of this study was to identify the causative mutations...Full Text Available

301

Morphology of wood species affecting wood-thermoplastic interaction: microstructure and mechanical adhesion  

Scientific Electronic Library Online (English)

Abstract in english The main objective of the research presented here is to relate anatomical features of wood species that affect the interactions between polymeric phases and performance of wood plastic composites (WPC). These interactions are related to the probable interlocking volume and surface area for stress transfer in a WPC. Composites were produced from different wood species and analyzed using SEM (scanning electron microscopy). Results showed that wood species with high interfac (more) ial areas may increase mechanical interlocking, reflected in the viscous constant of the Maxwell model. A complicating factor is that the relation of cell wall thickness-lumen diameter and the interconnectivity between wood cells in a wood, affect the potential for cell collapse. When wood cells collapse, the penetration of the thermoplastic into the wood structure is almost always ceased. The collapse of wood cells during extrusion-injection molding ...

2009-01-01

302

Molecular characterization of a unique retrovirus associated with a fish tumor.  

UK PubMed Central (United Kingdom)

The walleye dermal sarcoma is a mesenchymal tumor which seasonally affects up to 27% of adult walleye fish (Stizostedion vitreum). It arises multicentrically in the dermis, in which its development...Full Text Available

1992-01-01

303

Molecular Characterization of Aluminium (aluminum) Tolerance in Rye  

Science.gov (United States)

Aluminium (Al) toxicity, affecting around half of the world¿s arable land, severely hinders the ability of crop plants to utilize moisture and nutrients by restricting root growth and function. Among the cultivated cereals, rye is the most Al-tolerant and represents an important potential source of ...

304

Milk fever and alert downer cows: Does hypophosphatemia affect the treatment response?  

UK PubMed Central (United Kingdom)

The purpose of this prospective cohort study was to identify factors that place a dairy cow with uncomplicated milk fever (MF) at significant risk of becoming an alert downer cow (ADC) and to verify...Full Text Available

2007-05-01

305

Membrane filtration of food suspensions.  

UK PubMed Central (United Kingdom)

Factors affecting the membrane filtration of food suspensions were studied for 58 foods and 13 membrane filters. Lot number within a brand, pore size (0.45 or 0.8 micrometer), and time elapsed before...Full Text Available

1979-01-01

306

Medical records documentation of constipation preceding Parkinson disease  

UK PubMed Central (United Kingdom)

Objective:Parkinson disease (PD) may affect the autonomic nervous system and may cause constipation; however, few studies have explored constipation preceding the motor onset of...Full Text Available

2009-11-24

307

Mechanistic fracture criteria for the failure of human cortical bone  

Energy Technology Data Exchange (ETDEWEB)

A mechanistic understanding of fracture in human bone is critical to predicting fracture risk associated with age and disease. Despite extensive work, a mechanistic framework for describing how the underlying microstructure affects the failure mode in bone is lacking.

2002-12-13

308

Mass Treatment with Azithromycin for Trachoma Control: Participation Clusters in Households  

UK PubMed Central (United Kingdom)

BackgroundMass treatment to trachoma endemic communities is a critical part of the World Health Organization SAFE strategy. However, non-participation may not be at random, affecting...Full Text Available

309

Male-derived cuticular hydrocarbons signal sperm competition intensity and affect ejaculate expenditure in crickets  

UK PubMed Central (United Kingdom)

Female sexual promiscuity can have significant effects on male mating decisions because it increases the intensity of competition between ejaculates for fertilization. Because sperm production is costly,...Full Text Available

2009-01-22

310

MR findings in acute Lyme disease affecting the knee. A case report  

Energy Technology Data Exchange (ETDEWEB)

In this paper we report a case with primarily unspecific arthralgia after surgical therapy of hallux valgus deformity and consecutive reflex sympathetic dystrophy in which MR led to the diagnosis of Lyme disease. (orig.)

2002-07-01

311

Loss of lysophosphatidylcholine acyltransferase 1 leads to photoreceptor degeneration in rd11 mice  

UK PubMed Central (United Kingdom)

Retinal degenerative diseases, such as retinitis pigmentosa and Leber congenital amaurosis, are a leading cause of untreatable blindness with substantive impact on the quality of life of affected individuals...Full Text Available

2010-08-31

312

Life history plasticity after attaining a dietary threshold for reproduction is associated with protein storage in flesh flies  

UK PubMed Central (United Kingdom)

SummaryBody condition affects the timing and magnitude of life history transitions. Therefore, identifying proximate mechanisms...Full Text Available

2008-12-01

313

Life Span Extension in Mice by Food Restriction Depends on an Energy Imbalance12  

UK PubMed Central (United Kingdom)

In this study, our main objective was to determine whether energy restriction (ER) affects the rate of oxygen consumption of mice transiently or lastingly and whether metabolic rate plays a role in...Full Text Available

2009-03-01

314

Lead and ?-Aminolevulinic Acid Dehydratase Polymorphism: Where Does It Lead? A Meta-Analysis  

UK PubMed Central (United Kingdom)

BackgroundLead poisoning affects many organs in the body. Lead inhibits δ-aminolevulinic acid dehydratase (ALAD), an enzyme with two co-dominantly expressed alleles, ALAD1...Full Text Available

2007-01-01

315

Influence of Water Deficits on the Abscisic Acid and Indole-3-Acetic Acid Contents of Cotton Flower Buds and Flowers  

UK PubMed Central (United Kingdom)

A field experiment was conducted during the summer of 1988 to test the hypothesis that water deficit affects the abscisic acid (ABA) and indole acetic acid (IAA) concentrations in cotton (Gossypium...Full Text Available

1990-07-01

316

Indoleacetic Acid and Abscisic Acid Antagonism  

UK PubMed Central (United Kingdom)

The phytochrome-mediated attachment of mung bean (Phaseolus vulgaris L., var. Oklahoma 612) root tips on glass is quickly affected by indoleactic acid and abscisic acid at concentrations...Full Text Available

1973-01-01

317

Increased Sensitivity to Light-Induced Melatonin Suppression in Premenstrual Dysphoric Disorder  

UK PubMed Central (United Kingdom)

Increased sensitivity to light-induced melatonin suppression characterizes some, but not all, patients with bipolar illness or seasonal affective disorder. The aim of this study was to test...Full Text Available

2010-08-01

318

Impact of informed-choice invitations on diabetes screening knowledge, attitude and intentions: an analogue study  

UK PubMed Central (United Kingdom)

BackgroundDespite concerns that facilitating informed choice would decrease diabetes screening uptake, 'informed choice' invitations that increased knowledge did not affect attendance...Full Text Available

319

Identification of cell cycle-related regulatory motifs using a kernel canonical correlation analysis  

UK PubMed Central (United Kingdom)

BackgroundGene regulation is a key mechanism in higher eukaryotic cellular processes. One of the major challenges in gene regulation studies is to identify regulators affecting the...Full Text Available

320

Identification of Genes Affecting the Toxicity of Anti-Cancer Drug Bortezomib by Genome-Wide Screening in S. pombe  

UK PubMed Central (United Kingdom)

Bortezomib/PS-341/Velcade, a proteasome inhibitor, is widely used to treat multiple myeloma. While several mechanisms of the cytotoxicity of the drug were proposed, the actual mechanism remains elusive....Full Text Available

321

Identification and expression of differentially expressed genes in the hard clam, Mercenaria mercenaria, in response to quahog parasite unknown (QPX)  

UK PubMed Central (United Kingdom)

BackgroundThe hard clam, Mercenaria mercenaria, has been affected by severe mortality episodes associated with the protistan parasite QPX (Quahog Parasite Unknown)...Full Text Available

322

Ibuprofen lysine (NeoProfen) for the treatment of patent ductus arteriosus  

UK PubMed Central (United Kingdom)

Patent ductus arteriosus (PDA) affects approximately 31% of infants whose birth weight is between 501 and 1500 g. The ductus arteriosus is a blood vessel that allows blood to bypass the pulmonary vasculature...Full Text Available

2007-01-01

323

I. Cyclopeptide Alkaloids; II. Phycocyanobilipeptides.  

Science.gov (United States)

Several examples of the 14-membered, para-bridged ring system of the cyclopeptide alkaloids have been synthesized via an active ester cyclization. The yield of monomeric cyclopeptide varied from 1 to 33% and was affected by the amino acid substitution pat...

1979-01-01

324

Household Water Disinfection in Hurricane-Affected Communities of Louisiana: Implications for Disaster Preparedness for the General Public  

UK PubMed Central (United Kingdom)

Objectives. Thousands of Louisiana residents were asked to boil water because of widespread disruptions in electricity and natural gas services after Hurricane Rita. We sought to assess...Full Text Available

2007-04-01

325

Host plant shifts affect a major defense enzyme in Chrysomela lapponica  

UK PubMed Central (United Kingdom)

Chrysomelid leaf beetles use chemical defenses to overcome predatory attack and microbial infestation. Larvae of Chrysomela lapponica that feed on willow sequester plant-derived salicin...Full Text Available

2011-03-22

326

High-Risk Populations Identified in Childhood Cancer Survivor Study Investigations: Implications for Risk-Based Surveillance  

UK PubMed Central (United Kingdom)

Childhood cancer survivors often experience complications related to cancer and its treatment that may adversely affect quality of life and increase the risk of premature death. The purpose of this...Full Text Available

2009-05-10

327

Gray Wolves as Climate Change Buffers in Yellowstone  

UK PubMed Central (United Kingdom)

Understanding the mechanisms by which climate and predation patterns by top predators co-vary to affect community structure accrues added importance as humans exert growing influence over both climate...Full Text Available

2005-04-01

328

Genomic Expression Libraries for the Identification of Cross-Reactive Orthopoxvirus Antigens  

UK PubMed Central (United Kingdom)

Increasing numbers of human cowpox virus infections that are being observed and that particularly affect young non-vaccinated persons have renewed interest in this zoonotic disease. Usually causing...Full Text Available

329

Genome-wide linkage scan for contraction velocity characteristics of knee musculature in the Leuven Genes for Muscular Strength Study  

UK PubMed Central (United Kingdom)

The torque-velocity relationship is known to be affected by ageing, decreasing its protective role in the prevention of falls. Interindividual variability in this torque-velocity relationship is partly...Full Text Available

2008-09-01

330

Fungal spores: hazardous to health?  

UK PubMed Central (United Kingdom)

Fungi have long been known to affect human well being in various ways, including disease of essential crop plants, decay of stored foods with possible concomitant production of mycotoxins, superficial...Full Text Available

1999-06-01

331

Food deprivation and the role of estradiol in mediating sexual behaviors in meadow voles  

UK PubMed Central (United Kingdom)

Female mammals are particularly sensitive to changes in food availability. The mechanisms that affect sexual behavior and food intake are closely related to one another; chief among the mechanisms...Full Text Available

2007-02-28

332

Fiddler's Neck Accompanied by Allergic Contact Dermatitis to Nickel in a Viola Player  

UK PubMed Central (United Kingdom)

"Fiddler's neck" is an irritant contact dermatitis that frequently affects violin and viola players. The etiology of the skin changes associated with this condition are probably attributable to a combination...Full Text Available

2010-02-01

333

Fear and safety learning differentially affect synapse size and dendritic translation in the lateral amygdala  

UK PubMed Central (United Kingdom)

Fear learning is associated with changes in synapse strength in the lateral amygdala (LA). To examine changes in LA dendritic spine structure with learning, we used serial electron microscopy to re-construct...Full Text Available

2010-05-18

334

Factors Affecting Daughter Cells' Arrangement during the Early Bacterial Divisions  

UK PubMed Central (United Kingdom)

On agar plates, daughter cells of Escherichia coli mutually slide and align side-by-side in parallel during the first round of binary fission. This phenomenon has been previously attributed...Full Text Available

335

Exogenous Melatonin Administration Affects Self-grooming and Conspecific Odor Preferences in Long-Photoperiod Meadow Voles (Microtus pennsylvanicus)  

UK PubMed Central (United Kingdom)

For meadow voles, Microtus pennsylvanicus, seasonal differences exist in self-grooming and in odor preferences for conspecifics, two behaviors which facilitate sexual interactions...Full Text Available

2007-06-08

336

Evolutionary Trajectories of Beta-Lactamase CTX-M-1 Cluster Enzymes: Predicting Antibiotic Resistance  

UK PubMed Central (United Kingdom)

Extended-spectrum beta-lactamases (ESBL) constitute a key antibiotic-resistance mechanism affecting Gram-negative bacteria, and also an excellent model for studying evolution in real time. A shift in...Full Text Available

2010-01-01

337

Epigallocatechin-3-gallate affects the growth of LNCaP cells via membrane fluidity and distribution of cellular zinc*  

UK PubMed Central (United Kingdom)

Objective: To evaluate effects of epigallocatechin-3-gallate (EGCG) on the viability, membrane properties, and zinc distribution, with and without the presence of Zn2+, in human prostate...Full Text Available

2009-06-01

338

Empirically Defined Subtypes of Alcohol Dependence in an Irish Family Sample  

UK PubMed Central (United Kingdom)

Alcohol dependence (AD) is clinically and etiologically heterogeneous. The goal of this study was to explore AD subtypes among a sample of 1, 221 participants in the Irish Affected Sib Pair...Full Text Available

2010-03-01

339

Electrophysiological Remodeling in Heart Failure  

UK PubMed Central (United Kingdom)

Heart failure affects nearly 6 million Americans, with a half-million new cases emerging each year. Whereas up to 50% of heart failure patients die of arrhythmia, the diverse mechanisms underlying...Full Text Available

2010-04-01

340

Effects of job rotation and role stress among nurses on job satisfaction and organizational commitment  

UK PubMed Central (United Kingdom)

BackgroundThe motivation for this study was to investigate how role stress among nurses could affect their job satisfaction and organizational commitment, and whether the job rotation...Full Text Available

341

Effects of ambient temperature and scrotal fleece cover on scrotal and testicular temperatures in rams.  

UK PubMed Central (United Kingdom)

The objective was to determine scrotal and testicular temperatures in rams and how they are affected by ambient temperature (10 degrees C vs 25 degrees C) and scrotal fleece (densely fleeced vs shaved)....Full Text Available

1999-04-01

342

Effects of Perfluorocarbons on surfactant exocytosis and membrane properties in isolated alveolar type II cells  

UK PubMed Central (United Kingdom)

BackgroundPerfluorocarbons (PFC) are used to improve gas exchange in diseased lungs. PFC have been shown to affect various cell types. Thus, effects on alveolar type II (ATII) cells...Full Text Available

2010-01-01

343

Effects of Depot Medroxyprogesterone Acetate and 20 ?g Oral Contraceptives on Bone Mineral Density  

UK PubMed Central (United Kingdom)

OBJECTIVEHormonal contraceptives may adversely affect bone mineral density . However, racial differences and the reversibility of these changes are poorly understood....Full Text Available

2008-10-01

344

Effect of a Longitudinally Applied Voltage Upon the Growth of Zea mays Seedlings 1  

UK PubMed Central (United Kingdom)

The electrical parameters that affect young seedling growth were investigated. Voltages ranging from 5 to 40 volts were applied longitudinally along the mesocotyl region of 4-day old Zea mays...Full Text Available

1988-08-01

345

EFFECTS OF ALTERNATIVE NEW SOURCE PERFORMANCE STANDARDS ON FLUE GAS DESULFURIZATION SYSTEM SUPPLY AND DEMAND  

Science.gov (United States)

The report discusses the capabilities of equipment vendors to supply and install the quantity of flue gas desulfurization systems required to meet alternative standards for coal-fired steam generators. It analyzes limiting factors affecting supply capabilities (such as the availa...

346

Dynamic NMR effects in breast cancer dynamic-contrast-enhanced MRI  

UK PubMed Central (United Kingdom)

The passage of a vascular-injected paramagnetic contrast reagent (CR) bolus through a region-of-interest affects tissue 1H2O relaxation and thus MR image intensity. For longitudinal...Full Text Available

2008-11-18

347

Dosing Recommendations for Continuous Venovenous Hemodiafiltration with AN69 Filter Membranes and Prismaflex Dialyzers  

UK PubMed Central (United Kingdom)

Background:Continuous renal replacement therapy is used to manage fluid and solute imbalances in critically ill patients but may affect the clearance of concurrently administered...Full Text Available

2009-11-01

348

Does hypothalamic SIRT1 regulate aging?  

UK PubMed Central (United Kingdom)

In virtually all organisms, life expectancy is profoundly affected by caloric intake. For example, dietary restriction (DR; a feeding regimen of fewer calories compared to the ad libitum level without...Full Text Available

349

Does amiodarone affect heart rate by inhibiting the intracellular generation of triiodothyronine from thyroxine?  

UK PubMed Central (United Kingdom)

The hypothesis that the antiarrhythmic drug amiodarone slows down the heart rate by its inhibitory action on the intracellular conversion of thyroxine (T4) to 3,5,3' triiodothyronine (T3) was investigated....Full Text Available

1984-05-01

350

Do gender and torus mandibularis affect mandibular cortical index? A cross-sectional study  

Science.gov (United States)

BackgroundThe interactions between torus and several factors such as age, gender, and dental status have not been studied comprehensively. The purpose of this study was to determine the effect of gender on the mandibular cortical index (MCI) and to investigate a possible association between torus mandibularis (TM) and MCI.MethodsThe study consisted of 189 consecutive patients referred to Department of Oral Diagnosis and Radiology of Hacettepe University within 30 workdays. Patients who did not have systemic disorders affecting bone density were included; and the age, gender, dental status and existing TM of the patients were recorded. Morphology of the mandibular inferior cortex was determined according to Klemitti's classification on panoramic radiographs.ResultsMCI was affected by age and gender (P 0.05).ConclusionIn the study population, MCI was affected by age and gender. As age increased, semilunar defects could be ...

2007-10-30

351

Differential Regulation of PDE5 Expression in Left and Right Ventricles of Feline Hypertrophy Models  

UK PubMed Central (United Kingdom)

BackgroundThough long known to affect smooth muscle biology, recent studies indicate that phosphodiesterase 5 (PDE5) is also expressed in myocardium. Recognizing that the regulation...Full Text Available

352

Diabetes Enhances Periodontal Bone Loss through Enhanced Resorption and Diminished Bone Formation  

UK PubMed Central (United Kingdom)

Using a ligature-induced model in type-2 Zucker diabetic fatty (ZDF) rat and normoglycemic littermates, we investigated whether diabetes primarily affects periodontitis by enhancing bone loss...Full Text Available

2006-06-01

353

Development of a prototype clinical decision support tool for osteoporosis disease management: a qualitative study of focus groups  

UK PubMed Central (United Kingdom)

BackgroundOsteoporosis affects over 200 million people worldwide, and represents a significant cost burden. Although guidelines are available for best practice in osteoporosis, evidence...Full Text Available

354

Determinants of output in group and solo medical practice.  

UK PubMed Central (United Kingdom)

Certain structural and environmental factors other than technical combination of resources and firm size are hypothesized to affect medical practice output. Four groups of variables related to physician...Full Text Available

1976-01-01

355

Defects in succinate dehydrogenase in gastrointestinal stromal tumors lacking KIT and PDGFRA mutations  

UK PubMed Central (United Kingdom)

Carney-Stratakis syndrome, an inherited condition predisposing affected individuals to gastrointestinal stromal tumor (GIST) and paraganglioma, is caused by germline mutations in succinate dehydrogenase...Full Text Available

2011-01-04

356

DNA Damage during G2 Phase Does Not Affect Cell Cycle Progression of the Green Alga Scenedesmus quadricauda  

UK PubMed Central (United Kingdom)

DNA damage is a threat to genomic integrity in all living organisms. Plants and green algae are particularly susceptible to DNA damage especially that caused by UV light, due to their light dependency...Full Text Available

357

DHA supplementation for late onset Stargardt disease: NAT-3 study  

UK PubMed Central (United Kingdom)

Background:We analyzed the effects of a docosahexaenoic acid (DHA) supplementation in patients affected with late onset Stargardt disease (STGD).Methods:DHA...Full Text Available

2010-01-01

358

DHA Supplementation during Pregnancy and Lactation Affects Infants' Cellular but Not Humoral Immune Response  

UK PubMed Central (United Kingdom)

Background. It is currently recommended that diet of pregnant mothers contain 200–300 mg DHA/day. Aim. To determine whether DHA supplementation...Full Text Available

2011-01-01

359

Copy Number Alterations and Methylation in Ewing's Sarcoma  

UK PubMed Central (United Kingdom)

Ewing's sarcoma is the second most common bone malignancy affecting children and young adults. The prognosis is especially poor in metastatic or relapsed disease. The cell of origin remains elusive,...Full Text Available

2011-01-01

360

Contact Line Pinning by Microfabricated Patterns: Effects of Microscale Topography  

UK PubMed Central (United Kingdom)

We study how the microscale topography of a solid surface affects the apparent advancing and receding angles at the contact line of a liquid drop pinned to this surface. Photolithographic methods...Full Text Available

2009-05-05

361

Comparative analysis of differentially expressed genes in normal and white spot syndrome virus infected Penaeus monodon  

UK PubMed Central (United Kingdom)

BackgroundWhite spot syndrome (WSS) is a viral disease that affects most of the commercially important shrimps and causes serious economic losses to the shrimp farming industry worldwide....Full Text Available

362

Common variation in the ABO glycosyltransferase is associated with susceptibility to severe Plasmodium falciparum malaria  

UK PubMed Central (United Kingdom)

There is growing epidemiological and molecular evidence that ABO blood group affects host susceptibility to severe Plasmodium falciparum infection. The high frequency of common...Full Text Available

2008-02-15

363

Cloning and Analysis of a Candida albicans Gene That Affects Cell Surface Hydrophobicity  

UK PubMed Central (United Kingdom)

The opportunistic pathogenic yeast Candida albicans exhibits growth phase-dependent changes in cell surface hydrophobicity, which has been correlated with adhesion to host tissues....Full Text Available

2001-06-01

364

Chronic inflammation and estradiol interact through MAPK activation to affect TMJ nociceptive processing by trigeminal caudalis neurons  

UK PubMed Central (United Kingdom)

The mitogen-activated protein kinase/extracellular regulated kinase (MAPK/ERK) pathway plays a key role in mediating estrogen actions in the brain and neuronal sensitization during inflammation....Full Text Available

2009-12-29

365

Chondroitin sulphate structure affects its immunological activities on murine splenocytes sensitized with ovalbumin  

UK PubMed Central (United Kingdom)

Chondroitin sulphate (CS) is a glycosaminoglycan widely distributed in animal tissues, which has anti-inflammatory and chondroprotective properties. We reported previously that chondroitin 4-sulphate...Full Text Available

2004-08-15

366

Centromere-encoded RNAs are integral components of the maize kinetochore  

UK PubMed Central (United Kingdom)

RNA is involved in a variety of chromatin modification events, ranging from large-scale structural rearrangements to subtle local affects. Here, we extend the evidence for RNA–chromatin interactions...Full Text Available

2004-11-09

367

CHRONIC ETHANOL FEEDING AFFECTS PROTEASOME INTERACTING PROTEINS  

UK PubMed Central (United Kingdom)

Studies on alcoholic liver injury mechanisms show a significant inhibition of the proteasome activity. To investigate this phenomenon, we isolated proteasome complexes from the liver of rats...Full Text Available

2009-07-01

368

CENTRAL CORNEAL OPACIFICATION RESULTING FROM RECENT CHEMOTHERAPY IN CORNEAL DONORS  

UK PubMed Central (United Kingdom)

PurposeOcular surface disease following penetrating keratoplasty has been shown to increase patient morbidity and adversely affect graft survival. Five cases of dense central subepithelial...Full Text Available

2007-12-01

369

CANDU year in review  

Energy Technology Data Exchange (ETDEWEB)

The commissioning of four CANDU-600 reactors is discussed, with mention of some design features. The four are Point Lepreau, Gentilly-2, Wolsung and Cordoba reactors. The commissioning of Pickering-5 is also mentioned, and so are some events affecting other CANDU reactors.

1983-01-01

370

Burnout sensitivity of power MOSFETs operating in a switching converter  

International Nuclear Information System (INIS)

Heavy ion tests of a switching converter using power MOSFETs have allowed us to identify the main parameters which affect the burnout sensitivity of these components. The differences between static and dynamic conditions are clarified in this paper. (author). 7 refs., 16 figs., 3 tabs.

371

Blood conservation strategies to reduce the need for red blood cell transfusion in critically ill patients  

UK PubMed Central (United Kingdom)

Anemia commonly affects critically ill patients. The causes are multifactorial and include acute blood loss, blood loss from diagnostic testing and blunted red blood cell production. Blood transfusions...Full Text Available

2008-01-01

372

Biogeography of Tick-Borne Bhanja Virus (Bunyaviridae) in Europe  

UK PubMed Central (United Kingdom)

Bhanja virus (BHAV) is pathogenic for young domestic ruminants and also for humans, causing fever and affections of the central nervous system. This generally neglected arbovirus of the family Bunyaviridae...Full Text Available

2009-01-01

373

Bioaccumulation and toxicity of selenium compounds in the green alga Scenedesmus quadricauda  

UK PubMed Central (United Kingdom)

BackgroundSelenium is a trace element performing important biological functions in many organisms including humans. It usually affects organisms in a strictly dosage-dependent manner...Full Text Available

374

Bacterial Fouling in a Model Core System  

UK PubMed Central (United Kingdom)

We have used a sintered glass bead core to simulate the spaces and surfaces of reservoir rock in studies of the bacterial plugging phenomenon that affects waterflood oil recovery operations. The passage...Full Text Available

1985-03-01

375

Attitudes toward abortion among parents of children with cystic fibrosis.  

UK PubMed Central (United Kingdom)

BACKGROUND: DNA prenatal diagnosis for cystic fibrosis (CF) has been available for parents of affected children since late 1985. METHODS: Using anonymous questionnaires, we surveyed 395 parents of children...Full Text Available

1991-08-01

376

Association of B-Type Natriuretic Peptide Levels With Estimated Glomerular Filtration Rate and Congestive Heart Failure  

UK PubMed Central (United Kingdom)

Background: The causes of elevated B-Type natriuretic peptide (BNP) levels are multifactorial. Renal dysfunction has been shown to affect BNP levels in some studies and...Full Text Available

2010-03-01

377

Aquaculture Enclosures Relate to the Establishment of Feral Populations of Introduced Species  

UK PubMed Central (United Kingdom)

Many species introduced by humans for social and economic benefits have invaded new ranges by escaping from captivity. Such invasive species can negatively affect biodiversity and economies. Understanding...Full Text Available

378

Angiosarcoma of the of scalp with calvarium involvement in a 50-year-old African-American man.  

UK PubMed Central (United Kingdom)

BACKGROUND: Angiosarcoma of the scalp is a rare, aggressive, and deadly cancer that affects mainly elderly Caucasian men. OBJECTIVES: The insidious and masquerading presentation of angiosarcoma poses...Full Text Available

2004-11-01

379

Anatomical Reasons for the Discrepancies in Atrioventricular Block after Inferior Myocardial Infarction with and without Right Ventricular Involvement  

UK PubMed Central (United Kingdom)

The incidence of arrhythmias after acute myocardial infarction of the inferior wall varies with the affected segment and increases when there is right ventricular involvement. This paper provides a...Full Text Available

2009-01-01

380

Analysis of Failed Spinal Cord Stimulation Trials in the Treatment of Intractable Chronic Pain  

UK PubMed Central (United Kingdom)

ObjectiveThe purpose of this study is to identify the factors affecting the failure of trials (<50% pain reduction in pain for trial period) to improve success rate of spinal...Full Text Available

2008-02-01

381

An imbalance between apoptosis and proliferation contributes to follicular persistence in polycystic ovaries in rats  

UK PubMed Central (United Kingdom)

BackgroundCystic ovarian disease is an important cause of infertility that affects bovine, ovine, caprine and porcine species and even human beings. Alterations in the ovarian micro-environment...Full Text Available

382

An evaluation of the effectiveness of a community mentoring service for socially isolated older people: a controlled trial  

UK PubMed Central (United Kingdom)

BackgroundSocial isolation affects a significant proportion of older people and is associated with poor health outcomes. The current evidence base regarding the effectiveness of...Full Text Available

383

Allergy and Sensitization during Childhood Associated with Prenatal and Lactational Exposure to Marine Pollutants  

UK PubMed Central (United Kingdom)

BackgroundBreast-feeding may affect the risk of developing allergy during childhood and may also cause exposure to immunotoxicants, such as polychlorinated biphenyls (PCBs), which...Full Text Available

2010-10-01

384

Age affects over-marking of opposite-sex scent marks in meadow voles, Microtus pennsylvanicus  

UK PubMed Central (United Kingdom)

Models of age-related effects on behavior predict that among short-lived species younger adults are more attractive and attracted to opposite-sex conspecifics than are older adults, whereas...Full Text Available

2010-01-01

385

Affective and Personality Risk and Cognitive Mediators of Initial Adolescent Alcohol Use*  

UK PubMed Central (United Kingdom)

Objective:This study examined the role of cognitive factors—such as expectancies regarding the consequences of not drinking and perceptions of peer drinking—in...Full Text Available

2010-07-01

386

Adhesive capsulitis and dynamic splinting: a controlled, cohort study  

UK PubMed Central (United Kingdom)

BackgroundAdhesive Capsulitis (AC) affects patient of all ages, and stretching protocols are commonly prescribed for this condition. Dynamic splinting has been shown effective in...Full Text Available

387

Addressing the Question of the Effect of RBC Storage on Clinical Outcomes: The Red Cell Storage Duration Study (RECESS) (Section 7)  

UK PubMed Central (United Kingdom)

The question of whether storage of red blood cells (RBCs) alters their capacity to deliver oxygen and affects patient outcomes remains in a state of clinical equipoise. Studies of the changes...Full Text Available

2010-08-01

388

A new VCAN/versican splice acceptor site mutation in a French Wagner family associated with vascular and inflammatory ocular features  

UK PubMed Central (United Kingdom)

PurposeTo detail the highly variable ocular phenotypes of a French family affected with an autosomal dominantly inherited vitreoretinopathy and to identify the disease gene.MethodsSixteen...Full Text Available

389

A cost-utility analysis of nursing intervention via telephone follow-up for injured road users  

UK PubMed Central (United Kingdom)

BackgroundTraffic injuries can cause physical, psychological, and economical impairment, and affected individuals may also experience shortcomings in their post-accident care and...Full Text Available

390

A Review of the Pathophysiology and Novel Treatments for Erectile Dysfunction  

UK PubMed Central (United Kingdom)

Erectile dysfunction (ED) affects up to 50% of men between the ages of 40 and 70. Treatment with PDE-5 inhibitors is effective in the majority of men with ED. However, PDE-5 inhibitors are not effective...Full Text Available

2010-01-01

391

A NEW APPROACH TO UNDERSTANDING THE MOLECULAR MECHANISMS THROUGH WHICH ESTROGENS AFFECT COGNITION  

UK PubMed Central (United Kingdom)

Traditional approaches to the study of hormones and cognition have been primarily observational or correlational in nature. Because this work does not permit causal relationships to be identified,...Full Text Available

2010-10-01

392

A Genomewide RNAi Screen for Genes That Affect the Stability, Distribution and Function of P Granules in Caenorhabditis elegans  

UK PubMed Central (United Kingdom)

P granules are non-membrane-bound organelles found in the germ-line cytoplasm throughout Caenorhabditis elegans development. Like their “germ granule” counterparts in...Full Text Available

2009-12-01

393

A Case of Neurocysticercosis in Entire Spinal Level  

UK PubMed Central (United Kingdom)

Cysticercosis is the most common parasitic infection affecting the central nervous system. Spinal neurocysticercosis (NCC) is very rare compared with intracranial NCC and requires more aggressive management...Full Text Available

2010-10-01

394

Zonal, provincial, lithological, and geomorphic features of soil salinization in the Southern federal okrug of Russia  

British Library Electronic Table of Contents (United Kingdom)

The relationships between soil salinization and the zonal and provincial bioclimatic conditions, the lithological composition of the sediments, and the geomorphic features of the territory have been analyzed for the Southern federal okrug of Russia. It is shown that the lithological and geomorphic conditions (relief, salinity of parent materials, degree of drainage, and the depth of saline groundwater) play an important role in the distribution of salt-affected soils against the background of the more general regularities specified by the climate. The participation of salt-affected soils in the soil cover of the Southern federal okrug increases in the eastward direction from the forest-steppe zone to the semidesert zone in agreement with an increase in the aridity and continentality of the...

2011-01-01

395

Visual impact evaluation of a wind park in a Greek island  

Energy Technology Data Exchange (ETDEWEB)

The visual impact of wind turbines is one of the main factors affecting public acceptance of wind parks. This paper evaluates the visual impact of a wind park in Chania, Crete, using the Spanish method of evaluation. The outcomes are combined with the psychometric testing of the residents by the use of questionnaires and with the values of the Spanish method about various scenarios concerning the size of the wind park (double, half, one wind turbine). The results of the study prove that the quantification of the potential visual impact could minimize this, apparently, main reason that affects public acceptance. (author)

2009-04-15

396

The successful treatment of lupus pernio with the flashlamp pulsed dye laser.  

Science.gov (United States)

Lupus pernio of the nose is the most characteristic cutaneous lesion of sarcoidosis. It is cosmetically disfiguring and can be the cause of significant morbidity. In particular, the affected skin is often red or purple due to increased vasculature. It is particularly resistant to both surgical and medical therapy. We describe a patient with lupus pernio affecting her nose, which showed a dramatic improvement following treatment with the pulse dye laser (PDL). A biopsy taken after treatment showed the continued presence of sarcoidal granulomas. We therefore feel that treatment with the PDL is an effective tool in improving the cosmetic appearance of lupus pernio, but does not influence the underlying disease process. PMID:11360426

1999-01-01

397

The effect of mitochondrial dysfunction on cytosolic nucleotide metabolism  

DEFF Research Database (Denmark)

Several enzymes of the metabolic pathways responsible for metabolism of cytosolic ribonucleotides and deoxyribonucleotides are located in mitochondria. Studies described in this paper suggest dysfunction of the mitochondria to affect these metabolic pathways and limit the available levels of cytosolic ribonucleotides and deoxyribonucleotides, which in turn can result in aberrant RNA and DNA synthesis. Mitochondrial dysfunction has been linked to genomic instability, and it is possible that the limiting effect of mitochondrial dysfunction on the levels of nucleotides and resulting aberrant RNA and DNA synthesis in part can be responsible for this link. This paper summarizes the parts of the metabolic pathways responsible for nucleotide metabolism that can be affected by mitochondrial dysfunction.

2010-01-01

398

Microstructure/Mechanical property relationships in bar products of Beta-C trademark (Ti-3Al-8V-6Cr-4Mo-4Zr)  

International Nuclear Information System (INIS)

Beta-C trademark is a metastable beta titanium alloy which can be heat treated to achieve a good combination of properties such as tensile strength, fatigue strength, ductility and toughness, such as needed in aircraft fasteners. Variations in hot and cold processing parameters and solution heat treatment can affect the resultant grain structure which in turn affects subsequent aging response and mechanical properties. Several processing/heat treating routes on round bar are explored in this study to determine the effects on microstructure and various mechanical properties.

1993-02-21

399

Landslides affecting sedimentary characteristics of reservoir basin  

British Library Electronic Table of Contents (United Kingdom)

Typhoons Aere (2004) and Matsa (2005) caused high nephelometric turbidity in the Shihmen reservoir in northern Taiwan, jeopardizing the operation of the reservoir for several days, and ultimately impacting the living conditions and economy of the downstream residents. The torrential rains caused landslides and debris flows in upland areas, and flowed into riverbeds, likely contributing significantly to the suspended sediment yields in the reservoir. This investigation elucidates how upland landslides affect sediment attributes in the reservoir basin. Study methods including field observations, spatial analysis in GIS and aerial photo interpretation are adopted to trace the sediment sources and contributing factors to the landslide. Torrential rains induced landslides and debris-flows uplan...

2010-01-01

400

International law on nuclear liability - a critical approach  

Energy Technology Data Exchange (ETDEWEB)

The author discusses in detail the following topics: Compensation for domestic nuclear damage and for transfrontier nuclear damage - rule of formal equality of parties which belongs to the basic rule of civil law considering the position of domestic and foreign victims of a grave accident-juridical consequences of the preponderant role played by the state in the promotion, development and supervision of the nuclear industry-rationale for applying the concept of global limitation of liability in the law on nuclear liability and compensation - financial consequences of uncompensated nuclear damage, borne by the victims directly affected or spread over the whole community of the affected state? (HP)

1995-12-31

401

Gaussian models for genetic linkage analysis using complete high-resolution maps of identity by descent  

Energy Technology Data Exchange (ETDEWEB)

Gaussian-process models are developed to detect genetic linkage using complete high-resolution maps of identity by descent between affected relative pairs. Approximations are given for the significance level and power of the likelihood-ratio test of no linkage and for likelihood-ratio confidence regions for trait loci. The sample sizes required to detect linkage by using different classes of affected relative pairs are compared, and the problem of combining data from different classes of relatives is discussed. 23 refs., 2 figs.

1993-07-01

402

The role of the HCR system in the repair of lethal lesions of Bacillus subtilis phages and their transfecting DNA damaged by radiation and alkylating agents  

International Nuclear Information System (INIS)

The role of the HCR system in the repair of prelethal lesions induced by UV light, #gamma# radiation and alkylating agents was studied in the Bacillus subtilis SPP1 phage, its heat sensitive mutants (N3, N73 nad ts_1) and corresponding infectious DNA. The survival of phages and their transfecting DNA after treatment with UV light is substantially higher in hcr"+ cells than in hcr cells, the differences being more striking in intact phages than in their transfecting DNA's. Repair inhibitors reduce survival in hcr"+ cells: caffeine lowers the survival of UV-irradiated phage SPP1 in exponentially growing hcr"+ cells but has no effect on its survival in competent hcr"+ cells; acriflavin and ethidium bromide decrease the survival of the UV-irradiated SPP1 phage in both exponentially growing and competent hcr"+ cells to the level of survival observed in hcr cells; moreover, ethidium bromide lowers the number of infective centres in hcr"+ cells of the UV-irradiated DNA of ...

403

The neuronal nicotinic acetylcholine receptor {alpha}7 subunit gene: Cloning, mapping, structure, and targeting in mouse  

Energy Technology Data Exchange (ETDEWEB)

The neuronal nicotinic acetylcholine receptor {alpha}7 subunit is a member of a family of ligand-gated ion channels, and is the only subunit know to bind {alpha}-bungarotoxin in mammalian brain. {alpha}-Bungarotoxin binding sites are known to be more abundant in the hippocampus of mouse strains that are particularly sensitive to nicotine-induced seizures. The {alpha}7 receptor is highly permeable to calcium, which could suggest a role in synaptic plasticity in the nervous system. Auditory gating deficiency, an abnormal response to a second auditory stimulus, is characteristic of schizophrenia. Mouse strains that exhibit a similar gating deficit have reduced hippocampal expression of the {alpha}7 subunit. We have cloned and sequenced the full length cDNA for the mouse {alpha}7 gene (Acra-7) and characterized its gene structure. The murine {alpha}7 shares amino acid identity of 99% and 93% with the rat and human {alpha}7 subunits, respectively. Using an interspecies backcross panel, the ...

1994-09-01

404

The ATG1/ATG13 Protein Kinase Complex Is Both a Regulator and a Target of Autophagic Recycling in Arabidopsis.  

Science.gov (United States)

Autophagy is an intracellular recycling route in eukaryotes whereby organelles and cytoplasm are sequestered in vesicles, which are subsequently delivered to the vacuole for breakdown. The process is induced by various nutrient-responsive signaling cascades converging on the Autophagy-Related1 (ATG1)/ATG13 kinase complex. Here, we describe the ATG1/13 complex in Arabidopsis thaliana and show that it is both a regulator and a target of autophagy. Plants missing ATG13 are hypersensitive to nutrient limitations and senesce prematurely similar to mutants lacking other components of the ATG system. Synthesis of the ATG12-ATG5 and ATG8-phosphatidylethanolamine adducts, which are essential for autophagy, still occurs in ATG13-deficient plants, but the biogenesis of ATG8-decorated autophagic bodies does not, indicating that the complex regulates downstream events required for autophagosome enclosure and/or vacuolar delivery. Surprisingly, levels of the ATG1a and ATG13a ...

2011-10-01

405

Role of mitochondria in sulfolipid biosynthesis by Euglena chloroplasts  

Energy Technology Data Exchange (ETDEWEB)

Sulfate activation occurs in Euglena mitochondria the authors now find that the sulfate activating enzymes are absent from Euglena chloroplasts. Cells of mutant W/sub 10/BSmL lacking plastids also lack detectable sulfolipid (SL) when grown on /sup 35/SO/sub 4//sup 2 -/ indicating that SL is absent from the mitochondria and is exclusively in the plastids. Plastids alone will convert /sup 35/S-cysteine to /sup 35/SL in the presence of ATP and Mg/sup 2 +/; light is stimulatory. Under similar conditions, chloroplasts and mitochondria incubated together convert /sup 35/SO/sub 4//sup 2 -/ to plastid-localized /sup 35/SL but either organelle incubated alone fails to effect this conversion. Unlabeled cysteine blocks SL labeling from sulfate in the mixed incubation; since cysteine is formed from sulfate by Euglena mitochrondria, cysteine (and other compounds) may move from the mitochondrion to the chloroplast to provide the sulfo group for SL formation. Although ...

1987-04-01

406

Relationship of DNA repair processes to mutagenesis and carcinogenesis in mammalian cells. Progress report, November 1, 1979-October 31, 1980  

International Nuclear Information System (INIS)

The objective of this research is to determine the role of DNA repair in mutagenesis and carcinogenesis in mammalian cells. Use of the host-cell reactivation viral suicide enrichment procedure was initiated in the isolation of repair-deficient mutants. Lightly mutagenized BHK cells were infected with irradiated Herpes simplex virus (HSV); several radiation-sensitive strains were isolated among the survivors of the infection. The characterization of these strains is progressing and the enrichments are continuing. That alterations in the frequency of mutation of C3H/10T 1/2 cells, occurring as a result of holding the cells in a confluent state following treatment with ethylmethane sulfonate, parallel the alterations in the frequency of neoplastic transformation was found. The repair capabilities of BHK cells were found to be intermediate in comparison to repair-proficient and -deficient human cells with regard to the reactivation of HSV treated with various ...

407

One-step RNA pathogen detection with reverse transcriptase activity of a mutated thermostable Thermus aquaticus DNA polymerase.  

Science.gov (United States)

We describe the cloning and characterization of a mutated thermostable DNA polymerase from Thermus aquaticus (Taq) that exhibits an increased reverse transcriptase activity and is therefore designated for one-step PCR pathogen detection using established real-time detection methods. We demonstrate that this Taq polymerase mutant (Taq M1) has similar PCR sensitivity and nuclease activity as the respective Taq wild-type DNA polymerase. In addition, and in marked contrast to the wild-type, Taq M1 exhibits a significantly increased reverse transcriptase activity especially at high temperatures (>60 degrees C). RNA generally hosts highly stable secondary structure motifs, such as hairpins and G-quadruplexes, which complicate, or in the worst case obviate, reverse transcription (RT). Thus, RT at high temperatures is desired to weaken or melt secondary structure motifs. To demonstrate the ability of Taq M1 for RNA detection of pathogens, we performed TaqMan probe-based ...

2010-02-01

408

Novel method for differentiation between Trastuzumab and host adaptive response.  

Science.gov (United States)

Humoral immune response to human epidermal growth factor receptor 2 (HER-2/neu or ErbB-2) has been detected in sera of breast cancer patients and shown to be an appropriate prognostic marker (Taylor et al., 2007). However, since Trastuzumab (Herceptin) is a widely used monoclonal antibody as cancer therapy agent for tumors over-expressing HER-2, there is a need for an efficient way to detect host-generated antibodies against HER-2 without the confounding effect of Herceptin. Here we describe a screening method developed to decipher between host antibodies against HER-2 and that of Herceptin. By producing a series of truncation mutants within the epitope of Herceptin, we were able to inhibit this binding. We demonstrated also that by a three amino acid substitution (PPF?SSS) we were able to abrogate Herceptin binding while generating a highly conserved HER-2 extracellular domain (ECD). By producing a stable cell line that expresses this mutated form of the human ...

2011-06-08

409

Murine antibody response to oral infection with live aroA recombinant Salmonella dublin vaccine strains expressing filamentous hemagglutinin antigen from Bordetella pertussis.  

Science.gov (United States)

Two plasmids which express either nearly intact or truncated filamentous hemagglutinin (FHA) from Bordetella pertussis and which are marked with a tetracycline resistance (Tcr) gene were transformed into Salmonella dublin SL1438, an aroA deletion mutant intended for use as an attenuated oral vaccine against salmonellosis. These S. dublin recombinants, when fed to mice, induced serum immunoglobulin, immunoglobulin M (IgM), and sometimes IgA antibody responses to FHA and S. dublin. In addition, IgA antibodies against FHA were found in gut wash fluids. S. dublin carrying pDB2300, a multicopy plasmid encoding truncated FHA protein, induced a better antibody response than did S. dublin carrying pDB2000, a low-copy-number plasmid encoding full-sized FHA. Administration of tetracycline to mice enhanced the stability of recombinant plasmids, and tetracycline-treated mice developed higher anti-FHA titers. Although neither strain examined is suitable for use in a human oral ...

1990-08-01

410

Development of radiological emergency preparedness technology  

Energy Technology Data Exchange (ETDEWEB)

Large-scale field tracer experiments have been conducted on Ulchin, Wolsung and Daeduk sites for the purpose of validating FADAS and of analyzing the environmental characteristics around the nuclear sites. The most influential factor in atmospheric dispersion is the meteorological condition. During the experiment, meteorological data were measured on the release point and the selected positions among sampling points. Once radioactive materials are released to the atmosphere, members of public may be exposed through the environmental media such as air, soil and foods. Therefore, to protect the public, adequate countermeasures should be taken at due time for those exposure pathways. both processes, of justification and optimization are applied to a countermeasure simultaneously for decision-making. The work scope of Biological research for the radiation protection had contained the search of biological microanalytic methods for assessing the health effect by {gamma}-radiation and toxic ...

2000-04-01

411

Control of Electrostatic Interactions Between F-Actin And Genetically Modified Lysozyme in Aqueous Media  

Science.gov (United States)

The aim for deterministic control of the interactions between macroions in aqueous media has motivated widespread experimental and theoretical work. Although it has been well established that like-charged macromolecules can aggregate under the influence of oppositely charged condensing agents, the specific conditions for the stability of such aggregates can only be determined empirically. We examine these conditions, which involve an interplay of electrostatic and osmotic effects, by using a well defined model system composed of F-actin, an anionic rod-like polyelectrolyte, and lysozyme, a cationic globular protein with a charge that can be genetically modified. The structure and stability of actin-lysozyme complexes for different lysozyme charge mutants and salt concentrations are examined by using synchrotron x-ray scattering and molecular dynamics simulations. We provide evidence that supports a structural transition from columnar arrangements of F-actin held ...

2009-06-04

412

Characterization of the human lipoprotein lipase (LPL) promoter: Evidence of two cis-regulatory regions, LP-[alpha] and LP-[beta] of importance for the differentation-linked induction of the LPL gene during adipogenesis  

Energy Technology Data Exchange (ETDEWEB)

When preadipocytes differentiate into adipocytes, several differentiation-linked genes are activated. Lipo-protein lipase (LPL) is one of the first genes induced during this process. To investigate early events in adipocyte development, we have focused on the transcriptional activation of the LPL gene. For this purpose, we have cloned and fused different parts of intragenic and flanking sequences with a chloramphenicol acetyltransferase reporter gene. Transient transfection experiments and DNase I hypersensitivity assays indicate that several positive as well as negative elements contribute to transcriptional regulation of the LPL gene. When reporter gene constructs were stably introduced into preadipocytes, we were able to monitor and compare the activation patterns of different promoter deletion mutants at selected time points representing the process of adipocyte development. We could delimit two cis-regulatory elements important for gradual activation of the ...

1992-10-01

413

Atomic substitution reveals the structural basis for substrate adenine recognition and removal by adenine DNA glycosylase  

Science.gov (United States)

Adenine DNA glycosylase catalyzes the glycolytic removal of adenine from the promutagenic A {center_dot} oxoG base pair in DNA. The general features of DNA recognition by an adenine DNA glycosylase, Bacillus stearothermophilus MutY, have previously been revealed via the X-ray structure of a catalytically inactive mutant protein bound to an A:oxoG-containing DNA duplex. Although the structure revealed the substrate adenine to be, as expected, extruded from the DNA helix and inserted into an extrahelical active site pocket on the enzyme, the substrate adenine engaged in no direct contacts with active site residues. This feature was paradoxical, because other glycosylases have been observed to engage their substrates primarily through direct contacts. The lack of direct contacts in the case of MutY suggested that either MutY uses a distinctive logic for substrate recognition or that the X-ray structure had captured a noncatalytically competent state in lesion ...

2010-01-14

414

(/sup 14/C)sucrose uptake and labeling of starch in developing grains of normal segl barley  

Energy Technology Data Exchange (ETDEWEB)

Previous work showed that the segl mutant of barley (Hordeum vulgare o Betzes) did not differ from normal Betzes in plant growth, photosynthesis, or fertility, but it produced only shrunken seeds regardless of pollen source. To determine whether defects in sucrose uptake or starch synthesis resulted in the shrunken condition, developing grains of Betzes and segl were cultured in (/sup 14/C)sucrose solutions after slicing transversely to expose the endosperm cavity and free space. In both young grains (before genotypes differed in dry weight) and older grains (17 days after anthesis, when segl grains were smaller than Betzes), sucrose uptake and starch synthesis were similar in both genotypes on a dry weight basis. To determine if sucrose was hydrolyzed during uptake, spikes of Betzes and segl were allowed to take up (fructose-U-/sup 14/C)sucrose 14 days after anthesis and the radioactivity of endosperm sugars was examined during 3 hours of incubation. Whereas less ...

1984-01-01

415

Tumour control probability (TCP) for non-uniform activity distribution in radionuclide therapy  

Energy Technology Data Exchange (ETDEWEB)

Non-uniform radionuclide distribution in tumours will lead to a non-uniform absorbed dose. The aim of this study was to investigate how tumour control probability (TCP) depends on the radionuclide distribution in the tumour, both macroscopically and at the subcellular level. The absorbed dose in the cell nuclei of tumours was calculated for {sup 90}Y, {sup 177}Lu, {sup 103m}Rh and {sup 211}At. The radionuclides were uniformly distributed within the subcellular compartment and they were uniformly, normally or log-normally distributed among the cells in the tumour. When all cells contain the same amount of activity, the cumulated activities required for TCP = 0.99 (A-tilde{sub TCP=0.99}) were 1.5-2 and 2-3 times higher when the activity was distributed on the cell membrane compared to in the cell nucleus for {sup 103m}Rh and {sup 211}At, respectively. TCP for {sup 90}Y was not affected by different radionuclide distributions, whereas for {sup 177}Lu, it was slightly ...

2008-08-21

416

The Effect of Static Magnetic Forces on Water Contents and Photosynthetic Pigments in Sweet Basil Ocimum basilicum L. (Lamiaceae)  

International Nuclear Information System (INIS)

Three different magnetic regimes; aerial, surface and buried; each with three different forces, have been used to investigate their effects on the water contents and photosynthetic pigments of sweet basil plants (Ocimum basilicum L.). Two groups of sweet basil seeds, Ocimum basilicum L. have been cultivated, one under normal conditions and the second has been subdivided into three portion (aerial, surface and buried) to examine the effect of different magnetic forces coming from the three directions on the resulted plants. At all directions of magnets, water contents have been significantly affected by the magnetic forces. Chlorophyll A and carotene contents have been affected, as well, according to the three magnetic forces coming from soil surface regime only. Chlorophyll B did not significantly affected by differences magnetic forces in the three regimes, but it is affected by magnetism wherever its ...

417

Wetlands - A valuable resource for the '90s  

Energy Technology Data Exchange (ETDEWEB)

The nation's wetlands are a valuable resource serving many important natural, industrial, commercial and recreational functions. Wetlands protect shore areas from waves and storms; act as storage areas for flood waters; remove waste from water by trapping soils or silt which settle to the bottom; improve water quality and reduce pollution; are a commercial breeding ground for fish and other foods; and provide space for recreation and habitat for wildlife. They also are located in prime industrial development areas. Recent wetlands losses have caused much public outcry, politicking and regulatory efforts to prevent this trend. During the '90s, the public will see efforts to strike a proper balance regarding wetlands use. Today, wetlands protection is a permitting issue for nearly every major industrial or commercial development and expansion project. Wetlands permit requirements affect a range of business entities. Any project that involves ...

1993-04-15

418

The effects of short term and chronic exposure to tritiated drinking water on pre- and postnatal brain development  

International Nuclear Information System (INIS)

Ingestion of HTO during oocyte maturation and continued during pre-implantation time was found to depress decidual response. At birth these offspring also showed a decrease in brain cell number. When HTO was given during pregnancy only, the offspring at birth showed a similar deficit in brain cell number. Even so, we could not demonstrate a gross deficit in oocyte maturation when HTO was ingested during sexual maturity only; however, when further continued during pregnancy, the measured newborn parameters were most severely affected. In this group, cell-multiplication in the cerebrum was severely decreased, protein synthesis was decreased, and somatic growth was also highly significantly decreased. These experiments indicate that even a short-term exposure to HTO during early pregnancy (pre-implantation) alters normal development to such an extent that it is still observable at birth. During embryonic development, HTO affected the rate of ...

1980-11-26

419

The Effect of Applied Pressure During Feeding of Critical Cast Aluminum Alloy Components With Particular Reference to Fatigue Resistance  

Energy Technology Data Exchange (ETDEWEB)

the medium to long freezing range alloys of aluminum such as A356, A357, A206, 319 for example are known to exhibit dispersed porosity, which is recognized as a factor affecting ductility, fracture toughness, and fatigue resistance of light alloy castings. The local thermal environment, for example, temperature gradient and freezing from velocity, affect the mode of solidification which, along with alloy composition, heat treatment, oxide film occlusion, hydrogen content, and the extent to which the alloy contracts on solidification, combine to exert strong effects on the porosity formation in such alloys. In addition to such factors, the availability of liquid metal and its ability to flow through the partially solidified casting, which will be affect by the pressure in the liquid metal, must also be considered. The supply of molten metal will thus be controlled by the volume of the riser available for feeding the ...

2003-06-30

420

Host location behavior of Cotesia plutellae Kurdjumov (Hymenoptera: Braconidae) in ambient and moderately elevated ozone in field conditions  

Energy Technology Data Exchange (ETDEWEB)

In field O{sub 3}-enrichment experiments increased herbivore densities have been reported, which could be due to negatively affected host location behavior of natural enemies. We addressed the impact of doubling background O{sub 3} on the host location of the parasitoid Cotesia plutellae by conducting 24-h trials in an open-air O{sub 3}-fumigation system during two consecutive years. Two circles (radii 1.40 and 4.00 m) of Plutella xylostella-infested potted cabbage plants were placed in the O{sub 3} and ambient plots. Female wasps were released into each plot from the center, and observed 5 times over a 24-h period to assess their host location capability. Thereafter, plants were kept in laboratory conditions until larvae pupation to determine parasitism rates. No significant differences were detected between ambient and O{sub 3}-enriched environments either in the number of wasps found in the field, or in the percentages of parasitized larvae. This suggests that ...

2008-11-15

421

Effects of multi-ion irradiation on microstructural changes in lithium titanate  

Energy Technology Data Exchange (ETDEWEB)

The irradiation behavior of Li{sub 2}TiO{sub 3} under a fusion reactor environment was simulated by simultaneous irradiation of Li{sub 2}TiO{sub 3} by the triple ion beams and the respective single ion beams of O{sup 2+}, He{sup +} and H{sup +}. The microstructural changes in Li{sub 2}TiO{sub 3} caused by the irradiation were measured by FT-IR photoacoustic spectroscopy. The results suggest that the amount of TiO{sub 2} formed is proportional to the dpa and that the method of irradiation does not affect the dependence of formation of TiO{sub 2}. On the other hand, the amount of defects and/or radiolytic products generated by irradiation, which is considered to trap hydrogen near the surface, is found to be affected by the method of irradiation. Such phenomena are believed to affect the tritium release behavior from Li{sub 2}TiO{sub 3}, and durability of Li{sub 2}TiO{sub 3} and compatibility of Li{sub 2}TiO{sub 3} with other ...

2009-04-30

422

Effects of multi-ion irradiation on microstructural changes in lithium titanate  

International Nuclear Information System (INIS)

The irradiation behavior of Li_2TiO_3 under a fusion reactor environment was simulated by simultaneous irradiation of Li_2TiO_3 by the triple ion beams and the respective single ion beams of O"2"+, He"+ and H"+. The microstructural changes in Li_2TiO_3 caused by the irradiation were measured by FT-IR photoacoustic spectroscopy. The results suggest that the amount of TiO_2 formed is proportional to the dpa and that the method of irradiation does not affect the dependence of formation of TiO_2. On the other hand, the amount of defects and/or radiolytic products generated by irradiation, which is considered to trap hydrogen near the surface, is found to be affected by the method of irradiation. Such phenomena are believed to affect the tritium release behavior from Li_2TiO_3, and durability of Li_2TiO_3 and compatibility of Li_2TiO_3 with other components of the breeder blanket such as structural materials in the fusion ...

2009-04-30

423

Copy number and orientation determine the susceptibility of a gene to silencing by nearby heterochromatin in Drosophila  

Energy Technology Data Exchange (ETDEWEB)

The classical phenomenon of position-effect variegation (PEV) is the mosaic expression that occurs when a chromosomal rearrangements moves a euchromatic gene near heterochromatin. A striking feature of this phenomenon is that genes far away from the junction with heterochromatin can be affected, as if the heterochromatic state {open_quotes}spreads.{close_quotes} We have investigated classical PEV of a Drosophila brown transgene affected by a heterochromatic junction {approximately} 60 kb away. PEV was enhanced when the transgene was locally duplicated using P transposase. Successive rounds of P transpose mutagenesis and phenotypic selection produced a series of PEV alleles with differences in phenotype that depended on transgene copy number and orientation. As for other examples of classical PEV, nearby heterochromatin was required for gene silencing. Modifications of classical PEV by alterations at a single site are unexpected, and these ...

1996-02-01

424

An analysis of thinning status for CANDU feeder pipes  

Energy Technology Data Exchange (ETDEWEB)

The degradation status of outlet feeders in Wolsung unit 1 is analyzed using feeder thickness measurement data during overhaul period in 2000. DBs related to feeder thinning are made for parameters affecting dissolution rate and parameters affecting mass transfer rate. The initial thickness of feeder in Wolsung unit 1 is analyzed using initial thickness data of Wolsung unit 2, 3 and 4 as there are no initial thickness data of Wolsung unit 1. The initial thickness is varied as feeder diameter and bending degree, and CANDU feeders can be divided as 3 types for 2.5'' feeders and 3 types for 2'' feeders. The average initial thickness value for each type is defined as the initial thickness value of the type for Wolsung unit 1 feeders. Thinning rate is evaluated as the value dividing difference between initial thickness and minimum measured thickness by operation time. It is found that the distribution of thinning ...

2000-10-01

425

Advanced fuzzy logic controllers design and evaluation for buildings' occupants thermal-visual comfort and indoor air quality satisfaction  

Energy Technology Data Exchange (ETDEWEB)

The aim of this paper is to present and evaluate control strategies for adjustment and preservation of air quality, thermal and visual comfort for buildings' occupants while, simultaneously, energy consumption reduction is achieved. Fuzzy PID, fuzzy PD and adaptive fuzzy PD control methods are applied. The inputs to any controller are: the PMV index affecting thermal comfort, the CO{sub 2} concentration affecting indoor air quality and the illuminance level affecting visual comfort. The adaptive fuzzy PD controller adapts the inputs and outputs scaling factors and is based on a second order reference model. More specifically, the scaling factors are modified according to a sigmoid type function, in such a way that the measured variable to be as closer as possible to the reference model. The adaptive fuzzy PD controller is compared to a non-adaptive fuzzy PD and to an ON-OFF one. The comparison criteria are the ...

2001-07-01

426

The sensory quality of irradiated breaded poultry products  

International Nuclear Information System (INIS)

The colour, odour, flavour and texture of a breaded turkey escalope irradiated at doses of 2 and 4 kGy was assessed using a trained sensory panel. Irradiation did not affect raw appearance but during storage the irradiated breaded products became paler than the control samples. The off odour of the raw irradiated products was stronger than that of the unirradiated samples. Irradiation also affected the odour, flavour and texture of the cooked products. Although the trained panelists were able to detect significant differences between irradiated and unirradiated breaded turkey escalopes, the numerical differences between treatments were often quite small. It is possible that an untrained consumer group would be unable to detect these differences. Further research is needed with consumer groups to establish if irradiated breaded products are acceptable. (author). 10 refs, 3 tabs.

1994-07-01

427

The role of surfactant in synthesis of magnetic nanocrystalline powder of NiFe2O4 by sol-gel auto-combustion method  

British Library Electronic Table of Contents (United Kingdom)

In this work, a new sol-gel auto-combustion method has been performed to synthesize single phase nickel ferrite nanocrystalline powders by using n-cetyltrimethylammonium bromide, as a cationic surfactant. The gels were prepared from ferric and nickel nitrates and citric acid. Ammonia was used as pH adjusting agent as well. The effects of the surfactant on the after combustion calcination process and the reduction of the resulting powder crystallite size which affects the magnetic properties of the material were investigated by XRD and DTA/TGA techniques. The results showed that the ignition of the gels in air have a self-propagating behavior. Addition of surfactant to the starting solution affected the crystallite size of the synthesized powders and their phase constitution. The crystallit...

2008-01-01

428

The impact of solar flares and magnetic storms on humans  

International Nuclear Information System (INIS)

Three classes of solar emanations, namely, photon radiation from solar flares, solar energetic particles, and inhomogeneities in the solar wind that drive magnetic storms, are examined, and their effects on humans and technological systems are discussed. Solar flares may disrupt radio communications in the HF and VLF ranges. Energetic particles pose a special hazard at low-earth orbit and above, where they can penetrate barriers such as spacesuits and aluminum and destroy cells and solid state electronics. Energetic solar particles also influence terrestrial radio waves propagating through polar regions. Magnetic storms may disturb the operation of navigation instruments, power lines and pipelines, and satellites; they give rise to ionospheric storms which affect radio communication at all latitudes. There is also a growing body of evidence that changes in the geomagnetic field affect biological systems. 3 refs.

429

The Neural control of mood: The possible role of the adrenergic system in the medulla  

British Library Electronic Table of Contents (United Kingdom)

Mood in humans is a complex phenomenon that integrates emotion (e.g. happiness and sadness), cognition, perception, ideation, and action in a coherent manner. In bipolar disorder extremes of mood (up or down) occur outside the normal range, in which all the above functions are coherently affected. Mood is controlled by a series of separate but interactive brain circuits that involve much of the brain, but particularly the limbic system. The question addressed in this paper is whether the coordination of all these separate systems into one coherent functional mood is mediated by non-linear dynamics acting between these systems as equal participants; or whether it is affected by a single master regulator controlling the others. The possible roles, as master regulators, of non-linear dynamica...

2011-01-01

430

Study on regional production and economy of cobia Rachycentron canadum commercial cage culture  

British Library Electronic Table of Contents (United Kingdom)

In recent years, cobia has become an emerging farmed species in Asia due to its quick growth and high economic value. This study collects biological and economic data affecting the economic performance of cobia farming in three countries, namely Taiwan, China, and Vietnam. The data are collected by questionnaire sampling and analyzed by multivariate statistical analysis in order to compare the key factors affecting the production and economy of cobia farming in these three countries. The results show that Taiwan, China, and Vietnam have significant differences in input intensities and profitability. China has the highest input intensity (3372.42?TWD/m3), as its high stocking density increases feed input. Taiwan has the highest unit input cost (103.44?TWD/kg), as the high quality of the pro...

2011-01-01

431

Strikingly different penetrance of LHON in two Chinese families with primary mutation G11778A is independent of mtDNA haplogroup background and secondary mutation G13708A  

British Library Electronic Table of Contents (United Kingdom)

The penetrance of Lebers hereditary optic neuropathy (LHON) in families with primary mitochondrial DNA (mtDNA) mutations is very complex. Matrilineal and nuclear genetic background, as well as environmental factors, have been reported to be involved in different affected pedigrees. Here we describe two large Chinese families that show a striking difference in the penetrance of LHON, in which 53.3% and 15.0% of members were affected (Pin vivo mitochondrial oxidative metabolism as described in a previous study. Evolutionary analysis failed to indicate any putatively pathogenic mutation that cosegregated with G11778A in these two pedigrees. Our results suggest that the variable penetrance of LHON in the two Chinese families is independent of both their mtDNA haplotype background and a seconda...

2008-01-01

432

Stem growth habit affects leaf morphology and gas exchange traits in soybean  

British Library Electronic Table of Contents (United Kingdom)

Backgrounds and Aims The stem growth habit, determinate or indeterminate, of soybean, Glycine max, varieties affects various plant morphological and developmental traits. The objective of this study is to identify the effect of stem growth habit in soybean on the stomatal conductance of single leaves in relation to their leaf morphology in order to better understand the ecological and agronomic significance of this plant trait. Methods The stomatal conductance of leaves on the main stem was measured periodically under favourable field conditions to evaluate gmax, defined as the maximum stomatal conductance at full leaf expansion, for four varieties of soybean and their respective determinate or indeterminate near isogenic lines (NILs). Leaf morphological traits including stomatal density, ...

2009-01-01

433

Spermatozoa of Sharpsnout sea bream (Diplodus puntazzo) and European sea bass (Dicentrarchus labrax) express opioid receptors  

British Library Electronic Table of Contents (United Kingdom)

Endogenous opioid peptides (EOP) are molecules affecting many biological functions influencing productive and reproductive performance. They increase in stress conditions and interact with specific opioidergic receptors (OR): delta, kappa and mu. Several studies in other animal species suggest that they can also affect sperm cell motility. In fish the ionic external environment modulates the increase of intracellular calcium ion that is required to activate sperm motility after spawning. In mammals OR are functionally associated to calcium channels and in fishes calcium is highly required for fish sperm activation. In this study the presence of OR on spermatozoa of two Perciform fish, the sharpsnout seabream (Diplodus puntazzo, family Sparidae) and the European sea bass (Dicentrarchus labr...

2010-01-01

434

Radiographic features of Ewing's sarcoma of the bones of the hands and feet  

International Nuclear Information System (INIS)

The radiographic features of Ewing's sarcoma of the bones of the hands and feet are reviewed utilizing cases obtained from the Mayo Clinic patient files and the consultation files of Drs. D.C. Dahlin and K.K. Unni. This series consists of a total of 43 cases of pathologically proven Ewing's sarcoma involving the small bones of the hands and feet. The classic radiographic features of Ewing's sarcoma in the long bones, including lytic, permeative destruction, aggressive periosteal reaction, cortical violation, and a soft tissue mass, are also seen in the bones of the hands and feet, with similar frequency. These classic features are most commonly present in lesions affecting the short tubular bones. Lesions affecting the tarsal bones more often demonstrate atypical radiographic features. These atypical radiographic appearances may play a role in the reported delay in diagnosis of Ewing's sarcoma within the tarsal bones. (orig.)

2001-03-01

435

Positive effects of UV radiation on a calanoid copepod in a transparent lake: do competition, predation or food availability play a role?  

British Library Electronic Table of Contents (United Kingdom)

Zooplankton tolerant to ultraviolet radiation (UVR) could be indirectly affected by UVR through interactions with UV-sensitive species in the same ecosystem. In Lake Giles, Pennsylvania, USA, the calanoid copepod Leptodiaptomus minutus is more UVR tolerant than the cohabiting species Daphnia catawba and Cyclops scutifer. We asked whether L. minutus is affected by UV-induced mortality of a food competitor (D. catawba) or a predator of its nauplii (C. scutifer). We conducted two in situ enclosure experiments with six treatments: L. minutus alone, L . minutus + Daphnia and L. minutus + Cyclops in the presence and absence of UVR. There were few differences in survival among treatments in Experiment 1, which had enhanced food and a cumulative UVR (320 nm) dose of 9.3 kJ m-2. In Experiment 2, wh...

2006-01-01

436

Oral administration of circulating precursors for membrane phosphatides can promote the synthesis of new brain synapses  

British Library Electronic Table of Contents (United Kingdom)

Although cognitive performance in humans and experimental animals can be improved by administering omega-3 fatty acid docosahexaenoic acid (DHA), the neurochemical mechanisms underlying this effect remain uncertain. In general, nutrients or drugs that modify brain function or behavior do so by affecting synaptic transmission, usually by changing the quantities of particular neurotransmitters present within synaptic clefts or by acting directly on neurotransmitter receptors or signal-transduction molecules. We find that DHA also affects synaptic transmission in mammalian brain. Brain cells of gerbils or rats receiving this fatty acid manifest increased levels of phosphatides and of specific presynaptic or postsynaptic proteins. They also exhibit increased numbers of dendritic spines on post...

2008-01-01

437

Onset of epilepsy and menarche-Is there any relationship?  

British Library Electronic Table of Contents (United Kingdom)

SummaryPurposeWomen with epilepsy have increased frequency of reproductive health problems compared to women without epilepsy. In puberty, reproductive hormonal changes during sexual maturation may affect epilepsy and induce the debut of seizures as indicated in some studies. On the other hand, epileptic activity affects sex hormone function, which may induce alterations in pubertal endocrine maturation and thereby menarche age. We wanted to investigate the relation between epilepsy and menarche age in a larger population of female epilepsy patients.MethodsA retrospective, questionnaire study of a cohort of 265 female outpatients from three Norwegian hospitals and 142 controls, aged 18-45 years was conducted. Parameters regarding epilepsy and reproductive health issues were registered. Per...

2006-01-01

438

Mother and infant coordinate heart rhythms through episodes of interaction synchrony  

British Library Electronic Table of Contents (United Kingdom)

Animal studies demonstrated the powerful impact of maternal-infant social contact on the infant's physiological systems, yet the online effects of social interactions on the human infant's physiology remain poorly understood. Mothers and their 3-month old infants were observed during face-to-face interactions while cardiac output was collected from mother and child. Micro-analysis of the partners' behavior marked episodes of gaze, affect, and vocal synchrony. Time-series analysis showed that mother and infant coordinate heart rhythms within lags of less than 1s. Bootstrapping analysis indicated that the concordance between maternal and infant biological rhythms increased significantly during episodes of affect and vocal synchrony compared to non-synchronous moments. Humans, like other mamm...

2011-01-01

439

Modeling of a self-excited pulse combustor and stability analysis  

British Library Electronic Table of Contents (United Kingdom)

The major bottleneck for popularization and utilization of the conventional mechanical valve pulse combustors is the self-priming mode of gas supply. An aerodynamic valve (as against mechanical valve) self-excited pulse combustor of the Helmholtz-type with continuous supply of gas and air was designed and a mathematical model was established in this paper. The theoretical model employed well-stirred reactor model and a single step Arrhenius chemistry, and took those factors which might affect the combustion stability into account. The factors include the variation of the mass rate of the reactants affected by the pressure in the combustion chamber, the convective and radiation heat loss in the combustion chamber, and the heat transfer and wall friction in the tailpipe. The effect of wall t...

2011-01-01

440

Metal dusting behaviour of welded Ni-base alloys with different surface finish  

Energy Technology Data Exchange (ETDEWEB)

Welded Ni-base alloys and Alloy 800 were exposed under metal dusting conditions at 600 C and 650 C for up to 6000 hours. Alloy 800 was attacked very strongly already in the first days and Alloy 600 also rather soon and widespread, on both materials attack started mainly in the heat affected zone. Several surface states of Alloy 600: brushed, ground, sand-blasted and pickled were tried only grinding caused a modest delay and decrease of metal dusting attack. Generally the attack was less widespread but deeper at 650 C than at 600 C, also for Alloy 601 and 602. The latter alloys show minor, mainly local attack, but especially the welds are affected. TIG welding led to better resistance than hand-welding. (Abstract Copyright [2003], Wiley Periodicals, Inc.)

2003-10-01

441

Longer exaggerated male genitalia confer defensive sperm-competitive benefits in an earwig  

British Library Electronic Table of Contents (United Kingdom)

As evidence mounts that male genitalia can affect relative fertilisation success, the role that sexual selection has played in the rapid and divergent evolution of genitalia is becoming increasingly recognized. Unfortunately, the limited functional understanding of these complex structures and their interactions with the female reproductive tract often limit interpretation regarding their evolution. Here, we address this issue using the earwig Euborellia brunneri, where both the male intromittent organ and the female spermatheca are highly exaggerated in length yet structurally simple. In a double mating design, we use the sterile male technique to study how sperm precedence patterns are affected by male genital length, male age, and the size of the male sperm storage organ, the seminal ve...

2011-01-01

442

Investigation of factors affecting the diffusion of mobile telephone services: An empirical analysis for Vietnam  

British Library Electronic Table of Contents (United Kingdom)

This paper analyzes the diffusion of mobile telecommunications services in Vietnam and examines how telecommunications regulation and potential substitute/complement services affect the growth of the number of mobile telephone subscribers. Using a logistic diffusion model, it is found that fixed telephone services are a complement while data services have a negative relation to mobile telephone services in Vietnam. As for regulation, the policy of introducing competition has been found to be the most effective in influencing the adoption of mobile services. Another important result is that the estimated potential market is roughly 76% of the total population. The findings suggest that suitable regulation that guarantees competition in the mobile telecommunications market in a developing co...

2009-01-01

443

Integrated method of RS and GPR for monitoring the changes in the soil moisture and groundwater environment due to underground coal mining  

British Library Electronic Table of Contents (United Kingdom)

Mining affects the environment in different ways depending on the physical context in which the mining occurs. In mining areas with an arid environment, mining affects plants? growth by changing the amount of available water. This paper discusses the effects of mining on two important determinants of plant growth?soil moisture and groundwater table (GWT)?which were investigated using an integrated approach involving a field sampling investigation with remote sensing (RS) and ground-penetrating radar (GPR). To calculate and map the distribution of soil moisture for a target area, we initially analyzed four models for regression analysis between soil moisture and apparent thermal inertia and finally selected a linear model for modeling the soil moisture at a depth 10?cm; the relative error o...

2009-01-01

444

Influence of sodium chloride and sodium sulfate salinities on photosynthetic carbon assimilation in peanut  

International Nuclear Information System (INIS)

The effect of NaCl and Na_2SO_4 treatments on chlorophyll content, rate of "1"4C assimilation and products of photosynthesis in peanut (Arachish hypogaea L.) variety TMV-10 has been investigated. It was observed that chlorophyll content was affected mainly by NaCl, Na_2SO_4 treatment lowered the rate of photosynthetic "1"4CO_2 fixation. The analysis of labelled products revealed that the salts affect the carbon metabolism differently. The radioactivity was found to be accumulated in fractions of sugars and sugarphosphates in the leaves of NaCl treated plants. Na_2SO_4 treatment brought about considerable decline in labelling of sugars and an increase in labelling of amino acids and sugarphosphates. (orig.).

1980-01-01

445

Impacts of Imported Liquefied Natural Gas on Residential Appliance Components: Literature Review  

Energy Technology Data Exchange (ETDEWEB)

An increasing share of natural gas supplies distributed to residential appliances in the U.S. may come from liquefied natural gas (LNG) imports. The imported gas will be of a higher Wobbe number than domestic gas, and there is concern that it could produce more pollutant emissions at the point of use. This report will review recently undertaken studies, some of which have observed substantial effects on various appliances when operated on different mixtures of imported LNG. While we will summarize findings of major studies, we will not try to characterize broad effects of LNG, but describe how different components of the appliance itself will be affected by imported LNG. This paper considers how the operation of each major component of the gas appliances may be impacted by a switch to LNG, and how this local impact may affect overall safety, performance and pollutant emissions.

2009-12-09

446

How is Regeneration of Plants after Mowing Affected by Shoot Size in Two Species-Rich Meadows with Different Water Supply?  

British Library Electronic Table of Contents (United Kingdom)

Mowing a meadow is an example of an equalizing process that reduces differences among species by removing aboveground biomass approximately 5?cm above ground. This regular disturbance that affects all plants prevents competitive exclusion of small species and thus allows coexistence of numerous species differing in shoot size. In this paper we search for the mechanism behind this by comparing the shoot biomass of 41 common species in dry and wet species-rich meadows in mown and recently abandoned plots in June (before mowing) and in October. We asked the following questions: i) Do the plants differ in proportion of biomass lost by mowing? ii) Are the mown plants able to compensate for biomass lost by mowing? iii) Is the compensatory ability of mown plants related to their size? iv) Is the ...

2010-01-01

447

Helium release from the uranium-plutonium mixed oxide (MOX) fuel irradiated to high burn-up in a fast breeder reactor (FBR)  

International Nuclear Information System (INIS)

The helium releases were investigated in FBR fuel pins irradiated to high burn-up. The released amounts increased with the increase of burn-up, but no burn-up dependence of their release fraction to total amounts generated was seen. The contents of "2"4"1Am in fuel pellets affected the released amounts. The effect of released helium on the internal pressure in fuel pins was smaller in FBR fuel pins than in LWR fuel pins, but released helium in fuel pins containing increased amounts of "2"4"1Am, which are anticipated in future FBRs, is expected to affect the internal pressure.

2010-06-01

448

Growth and transpiration of maize and winter wheat in response to water deficits in pots and plots  

British Library Electronic Table of Contents (United Kingdom)

Pots used for experiments conducted on plants grown in them create rooting environments that are affected by limited soil volume, which can affect various physiological processes, including transpiration, and plant growth. However, the applicability of results from pot experiments to the field has received limited attention. The objective of this study was to compare the growth and transpiration of maize (Zea mays L.) and winter wheat (Triticum aestivum L.) when grown in pots and field plots under various constant water deficits. The experiments were conducted under similar environmental conditions for both pots and plots. Transpirational responses at both transient (RTTr) and daily (RDTr) time scales to a decreasing fraction of available soil water (FASW) were analyzed. For a comparable F...

2011-01-01

449

Fast neutron irradiation induced changes in the optical and thermal properties of modified polyvinyl chloride  

International Nuclear Information System (INIS)

The effect of both dopant and neutron radiation on the optical and thermal properties of polyvinyl chloride (PVC) has been studied. The doped samples with Pb and Cd were irradiated with a 14 MeV-neutron fluence in the range 7-28.8 x 10"9 n/cm"2. The optical energy gap E_o_p exhibits a significant dependence on the type of additive and the neutron irradiation fluence. The specific heat at constant pressure C_p showed a nonmonotonical change with radiation fluence. The results of this study show that PVC:Pb behaves as a crystalline structure which is only slightly affected by neutron irradiation, while PVC:Cd is highly affected. (author).

1994-11-01

450

Effects of wildfires on environmental variability: a comparative analysis using different spectral indices, patch metrics and thematic resolutions  

British Library Electronic Table of Contents (United Kingdom)

Knowledge on environmental variability and how it is affected by disturbances is crucial for understanding patterns of biodiversity and determining adequate conservation strategies. The aim of this study is to assess environmental variability in patches undergoing post-fire vegetation recovery, identifying trends of change and their relevant drivers. We particularly evaluate: the value of three spectral indices derived from Landsat satellite data [Normalized Burn Ratio (NBR), Normalized Difference Vegetation Index (NDVI) and Wetness Component of the Tasseled Cap Transformation (TCW)] for describing secondary succession; the effectiveness of three metrics (diversity, evenness and richness) as indicators of patch variability; and how thematic resolution can affect the perception of environme...

2010-01-01

451

Effect of #gamma#-irradiation on commercial polypropylene based mono and multi-layered retortable food packaging materials  

International Nuclear Information System (INIS)

Irradiation processing of food in the prepackaged form may affect chemical and physical properties of the plastic packaging materials. The effect of #gamma#-irradiation doses (2.5-10.0 kGy) on polypropylene (PP)-based retortable food packaging materials, were investigated using Fourier transform infrared (FTIR) spectroscopic analysis, which revealed the changes happening to these materials after irradiation. The mechanical properties decreased with irradiation while oxygen transmission rate (OTR) was not affected significantly. Colour measurement indicated that Nylon 6 containing multilayer films became yellowish after irradiation. Thermal characterization revealed the changes in percentage crystallinity.

2007-07-01

452

Economic assessment of combined cycle gas turbines in Australia Some effects of microeconomic reform and technological change  

International Nuclear Information System (INIS)

Australian electricity markets and natural gas markets are undergoing rapid reform. Choosing among electricity generation modes is a key issue. Such choices are affected by expectations about the future structure of these markets and future technologies, and how they affect costs and emissions. In the research reported in this paper, the MARKAL model of the Australian energy system is used to evaluate the competitive position of natural gas fired combined cycle gas turbines (CCGTs) in the energy sector as a whole. Competing in the sector are large-scale electricity generation technologies such as refurbished existing coal fired stations and advanced forms of coal fired generation. The modelling incorporates new data on electricity supply technologies and options.

2003-02-01

453

Development and credibility assessment of a metamodel relating water table depth to agricultural production  

British Library Electronic Table of Contents (United Kingdom)

Phreatic groundwater pumping is affecting water availability for crops in areas with a shallow water table. This can reduce crop growth and so affect farm income. There is a need for a generic and transparent method to assess the agricultural damage caused by water table drawdown. This paper proposes such a method that consists of 'damage tables' relating agricultural production losses to the groundwater regime for different soil/crop combinations found in Northern Belgium. The damage tables are constructed based on numerous simulations with the agrohydrological model SWAP, in which the bottom boundary conditions are gradually changed to reflect different groundwater regimes. The credibility of the resulting metamodel is assessed in three ways: using (1) field data, (2) an existing local e...

2010-01-01

454

Detrital processing in streams exposed to acidic precipitation in the Central Appalachian Mountains  

International Nuclear Information System (INIS)

Continuing high rates of acidic deposition in the eastern United States may lead to long-term effects on stream communities, because sensitive catchments are continuing to lose anions and cations. A two-year study of the effects of pH and associated water chemistry variables on detrital processing in three streams with different bedrock geology in the Monongahela National Forest, West Virginia were investigated. Leaf pack processing rates and macroinvertebrate colonization and microbial biomass (ATP concentration) on the packs in the three stream were compared. It was found that macroinvertebrate and microbial communities differed both among streams that differed in their capacity to buffer the effects of acidic precipitation and among years in the same stream; these differences in biotic communities were not large enough to affect rates of leaf processing between the two years of the study, but they did significantly affect processing rates ...

455

Detection of the first gross CDC73 germline deletion in an HPT-JT syndrome family  

British Library Electronic Table of Contents (United Kingdom)

Abstract Hereditary primary hyperparathyroidism (HPT) may develop as a solitary endocrinopathy (FIHP) or as part of multiple endocrine neoplasia Type 1, multiple endocrine neoplasia Type 2A, or hereditary HPT-jaw tumor syndrome. Inactivating germline mutations of the tumor suppressor gene CDC73 account for 14 and 50% of all FIHP and HPT-JT patients, respectively, and have also been found in almost 20% of apparently sporadic parathyroid carcinoma patients. Although more than 60 independent germline mutations have been described, to date no rearrangement affecting the CDC73 locus has been identified. By means of multiplex-PCR we found a large germline deletion affecting the whole gene in a two-generation HPT-JT family. Subsequently array-CGH and specific PCR analysis determined that the muta...

2011-01-01

456

Crop diversification and trade liberalization: Linking global trade and local management through a regional case study  

British Library Electronic Table of Contents (United Kingdom)

Some models anticipate that liberalized agricultural trade will lead to increased crop diversity, while other models make the opposite claim. These positions were explored in southwestern British Columbia, Canada where, between 1992 and 1998, government subsidies and other measures designed to protect horticultural farmers were lifted, exposing these farmers to foreign competition. Public hearings on the future of agriculture provided an opportunity to tap the knowledge and experience of people affected by this transition. Analysis of transcripts from these hearings, which was confirmed by industry data, shows that trade liberalization has led to the loss of the local fruit and vegetable processing industry. Stakeholders saw the loss as a major factor affecting the choice of crops grown lo...

2006-01-01

457

Comparison of a cavity solar receiver numerical model and experimental data  

Energy Technology Data Exchange (ETDEWEB)

Results from a numerical model of axisymmetric solar cavity receivers are compared with experimental data for tests of a novel test bed receiver in the Saudi National Laboratories solar furnace. The computed energy transfer rates and temperatures are compared with the experimental data for different receiver geometries, aperture sizes, and operating conditions. In general, the agreement between the numerical model and the experimental data is better for the small-to-midsized apertures than for the large apertures. The analysis indicates that for the larger apertures, the convective heat losses are overpredicted. It also suggests that these losses could be better characterized. Sensitivity analyses show that both the total solar energy input rate and the convective heat-loss coefficient significantly affect the receiver thermal performance and that the distribution of the input solar flux significantly affects the temperature distribution in the ...

1990-08-01

458

Comparative evaluation of /sup 99m/Tc GH, /sup 99m/TcO_4, and /sup 99m/Tc DTPA as brain imaging agents  

International Nuclear Information System (INIS)

The brain imaging properties of /sup 99m/Tc glucoheptonate, /sup 99m/Tc pertechnetate, and /sup 99m/Tc DPTA are compared. Results demonstrate that optimum images are obtained at 90, 180, and 180 min., for /sup 99m/Tc GH, /sup 99m/Tc DTPA, and /sup 99m/Tc pertechnetate, respectively. The former two images are not affected by prior bone imaging with /sup 99m/Tc pyrophosphate, while /sup 99m/Tc pertechnetate images are adversely affected. /sup 99m/Tc glucoheptonate appears to be the superior agent for brain imaging, followed by /sup 99m/Tc DTPA and /sup 99m/Tc pertechnetate.

459

Chronic maternal stress affects growth, behaviour and hypothalamo?pituitary?adrenal function in juvenile offspring  

British Library Electronic Table of Contents (United Kingdom)

Maternal stress during pregnancy, particularly that combined with low socioeconomic status (SES), has been linked to an increased risk for impaired behavioural and emotional development and affective disorders in children. In animal models, acute periods of prenatal stress have profound effects on hypothalamo-pituitary-adrenal (HPA) function and behaviour. However, few studies have determined the impact of chronic exposure to stress in animal models. The objective of this study was to determine the effects of chronic maternal stress (CMS) during the 2nd half of pregnancy and nursing on growth, locomotor behaviour and HPA axis function in juvenile guinea pig offspring. Pregnant guinea pigs were exposed to a random combination of variable stressors every other day over the 2nd half of gestat...

2008-01-01

460

Chernobyl accident: the crisis of the international radiation community  

Energy Technology Data Exchange (ETDEWEB)

The information given in the present report about the Chernobyl accident and its radiological consequences indicates a serious crisis of the international radiation community. The following signs of this crises can be discerned: The international radiation community did not recognize the real reasons of the accident for a long time. It could not make a correct assessment of the damage to the thyroid of the affected populations of Belarus, Russia and the Ukraine. Up to present time it rejects the reliable data on hereditary malformations. It is not able to accept reliable data on the increase in the incidence in all categories of people affected by the Chernobyl accident. The international radiation community supported the Soviet authorities in their attempts to play down the radiological consequences of the Chernobyl accident for a long time. (author)

1998-03-01

461

Chemistry between crosslinks affects the properties of peptide hydrogels  

British Library Electronic Table of Contents (United Kingdom)

Protein hydrogels were prepared by substituting ovalbumin with different concentrations of ethyl vinyl sulfone (EVS) or acrylic acid (AA) and crosslinking with divinyl sulfone (DVS). Fourier transform-infrared (FT-IR) spectroscopic studies confirmed the addition of EVS, AA, and DVS onto the protein. Swelling was assessed as a function of pH in the range of 2.5 to 9.4 and ionic strength. The elastic modulus of the gels was determined in shear and compression. Stress relaxation was assessed in compression. The substituent highly affected swelling and modulus with both hydrogels displaying non-Gaussian behavior in the range of hydrogel environments studied. Acrylic acid substituted ovalbumin exhibited a decreasing modulus with increasing swelling behaving as a polyelectrolyte with low added s...

2011-01-01

462

Bottom-up carbon subsidies and top-down predation pressure interact to affect aquatic food web structure  

British Library Electronic Table of Contents (United Kingdom)

Human impacts such as eutrophication, overexploitation and climate change currently threaten future global food and drinking water supplies. Consequently, it is important that we understand how anthropogenic resource (bottom-up) and consumer (top-down) manipulations affect aquatic food web structure and production. Future climate changes are predicted to increase the inputs of terrestrial dissolved organic carbon to lakes. These carbon subsidies can either increase or decrease total basal production in aquatic food webs, depending on bacterial competition with phytoplankton for nutrients. This study examines the effects of carbon subsidies (bottom-up) on a pelagic community exposed to different levels of top-down predation. We conducted a large scale mesocosm experiment in an oligotrophic ...

2011-01-01

463

Artificial neural network modeling of physicochemical changes of shrimp during boiling  

British Library Electronic Table of Contents (United Kingdom)

Frozen boiled shrimp and dried shrimp are among the high-value fishery products of Thailand. During the production of these products boiling is one of the most important steps that affects significantly the product physicochemical properties, especially the quantity and quality of proteins, which in turn affect other apparent properties perceived by consumers. The protein changes are, however, difficult to evaluate comparing to other typical physical properties of shrimp. The objective of this study was therefore to develop an artificial neural network (ANN) model to predict the protein changes of shrimp in terms of protein loss and protein denaturation as a function of the boiling conditions, namely, concentration of salt solution and boiling time, as well as a rather easily determined ch...

2012-01-01

464

Apheresis affects bone and mineral metabolism  

British Library Electronic Table of Contents (United Kingdom)

Background: Apheresis is a procedure to selectively obtain blood components. For the collection process citrate is routinely used. It inhibits coagulation by binding to ionized calcium and leads to metabolic alkalosis. Objective: Whether regular apheresis affects bone and mineral metabolism is unknown. The intention of this study was to investigate 1) the acute effects of apheresis on acid-base balance, bone and mineral metabolism and 2) to compare bone mineral density (BMD) at the lumbar spine and hip of donors to matched control subjects. Design: In this open, observational, single-center, cross-sectional study we enrolled 102 regular plasma and thrombocyte donors to pursue objective 1) and compared those to 102 matched controls (CTR) for objective 2). Results: Platelet donation led to s...

2010-01-01

465

An empirical analysis of factors affecting adolescent attachment in adoptive families with homosexual and straight parents  

British Library Electronic Table of Contents (United Kingdom)

Data were collected on 154 adoptive families with gay/lesbian and straight adoptive parents (154 parent respondents and 210 adolescent respondents). This study was principally interested in factors affecting adolescent attachment including parent sexual orientation, adolescent and parent life satisfaction, and parent level of relationship satisfaction with their adopted child as well as other key parent, child and adoption characteristics. The results suggest that higher level of adopted adolescent attachment to parents is not related to adoptive parent sexual orientation. Adolescent attachment to parents is related to adolescent life satisfaction; parent level of relationship satisfaction with their adopted child, number of placements prior to adoption, and adolescent's current age. Adole...

2009-01-01

466

Alien arthropod predators and parasitoids: interactions with the environment  

British Library Electronic Table of Contents (United Kingdom)

Many species of entomophagous arthropods have been introduced either intentionally (through the practice of biological control) or unintentionally to new regions. We examine interactions of these aliens with their new environments in the context of rapid global change linked to human activity. We consider effects of such interactions on establishment and spread of the alien species and effects on indigenous biota and ecosystems. Major elements of global change that affect alien-environment interactions include landscape modifications by humans (e.g., cultivation, habitat loss and fragmentation) and increases in atmospheric CO2 and other gases resulting in climate change and other effects (e.g., changes in food quality for herbivores that affect higher trophic levels as well). Alien arthrop...

2011-01-01

467

Affective Distress and Amputation-Related Pain Among Older Men with Long-Term, Traumatic Limb Amputations  

British Library Electronic Table of Contents (United Kingdom)

Psychological distress and postamputation pain were investigated in a sample of 582 males with long-term limb amputations (mean time since amputation 639.3 months, standard deviation 166.1; range 240-784 months). Prevalence of significant depressive symptoms (Hospital Anxiety and Depression Scale [HADS]-D score?8) was 32.0%, and 34.0% of respondents met the screening criterion for clinical anxiety (HADS-A score?8). Nearly one quarter (24.6%) of respondents reported significant post-traumatic psychological stress symptoms (Impact of Event Scale scores?35). In total, 87.8% experienced either phantom or residual limb pain. Affective distress scores differed according to the respondents' type of pain experience. Respondents who experienced residual limb pain reported significantly hig...

2006-01-01

468

Local chromatin structure of heterochromatin regulates repeatedDNA stability, nucleolus structure, and genome integrity  

Energy Technology Data Exchange (ETDEWEB)

Heterochromatin constitutes a significant portion of the genome in higher eukaryotes; approximately 30% in Drosophila and human. Heterochromatin contains a high repeat DNA content and a low density of protein-encoding genes. In contrast, euchromatin is composed mostly of unique sequences and contains the majority of single-copy genes. Genetic and cytological studies demonstrated that heterochromatin exhibits regulatory roles in chromosome organization, centromere function and telomere protection. As an epigenetically regulated structure, heterochromatin formation is not defined by any DNA sequence consensus. Heterochromatin is characterized by its association with nucleosomes containing methylated-lysine 9 of histone H3 (H3K9me), heterochromatin protein 1 (HP1) that binds H3K9me, and Su(var)3-9, which methylates H3K9 and binds HP1. Heterochromatin formation and functions are influenced by HP1, Su(var)3-9, and the RNA interference (RNAi) pathway. My thesis project investigates how ...

2007-05-05

469

Vegetable oil extraction using liquid CO/sub 2/  

Energy Technology Data Exchange (ETDEWEB)

SC-CO/sub 2/ extraction of oil from peanuts is an alternative to hexane extraction or the mechanical oil press. Oil was successfully extracted using SC-CO/sub 2/ at temperatures of 25-120/sup 0/C and pressures of 140 -690 Bar. Pressure, temperature and particle size affected the extraction of oil. In the range studied, the highest values of temperature and pressure gave highest solubilities.

1986-01-01

470

The Sigma Factor AlgU (AlgT) Controls Exopolysaccharide Production and Tolerance towards Desiccation and Osmotic Stress in the Biocontrol Agent Pseudomonas fluorescens CHA0  

UK PubMed Central (United Kingdom)

A variety of stress situations may affect the activity and survival of plant-beneficial pseudomonads added to soil to control root diseases. This study focused on the roles of the sigma factor AlgU...Full Text Available

2001-12-01

471

The Challenge Ahead for Rural Schools.  

Science.gov (United States)

Describes general trends in the quality of rural education: equivalent rural and urban performance on national standardized tests and high school graduation rates but continuing rural deficits in college attendance and teacher qualifications. Discusses the below-average performance of southern rural schools, factors affecting rural students' college attendance, and the influence of changing labor markets. (SV)

1999-12-01

472

Tachyons in #sigma#-models  

International Nuclear Information System (INIS)

We analyze the O(N) symmetric #sigma#-model in four dimensions with and without external source in the large-N approximation. We show that tachyons appear in these models only if cut-off effects are neglected. Including these effects and incorporating the triviality of the #sigma#-model, we demonstrate that no tachyon pole exists. We discuss how our results affect recent ideas to circumvent the 'no-loose-corollary' for W_L W_L scattering. (orig.).

473

Super-heavy Quarkonia and the Gluon Condensate  

CERN Document Server

The early idea that a non-perturbative gluon condensate affects the spectrum of heavy quarks is revisited in the light of modern simulation techniques. We evaluate the low lying spectrum of bound states of two heavy quarks for large hypothetical quark mass, m_Q > m_b, using non-relativistic QCD and compare with other models to test the consistency.

1999-01-01

474

Spontaneous mutations affecting the host range of the B77 strain of avian sarcoma virus involve type-specific changes in the virion envelope antigen.  

UK PubMed Central (United Kingdom)

Previously it was shown that the host-range gene of the Bratislava strain of avain sarcoma virus (B77 virus) spontaneously mutates with a very high rate. The wild-type B77 virus called B77 virus-II,...Full Text Available

1977-01-01

475

Rural Education in Tasmania.  

Science.gov (United States)

Addresses factors affecting rural education in Tasmania (Australia), including population trends, financial support, student enrollment, socioeconomic status of rural residents, and geographic isolation. Issues that need to be addressed include the status of small schools in Tasmania, costs associated with transporting students, staffing, low student retention, and limited availability of special services. (LP)

1996-12-01

476

Review of combustion processes in the dual fuel engine: the gas diesel engine  

Energy Technology Data Exchange (ETDEWEB)

This review examines the characteristics of the compression-ignition type of dual-fuel engine and the combustion phenomena that affect these characteristics. The specific areas covered involve normal combustion processes, the nature and origin of engine knock, operation with LNG, and the effect of intake temperature on combustion efficiency and exhaust composition.

1980-01-01

477

Reductive dechlorination of chlorinated solvents by zero-valent iron, iron oxide and iron sulfide minerals  

Energy Technology Data Exchange (ETDEWEB)

The degradation of chlorinated solvents by reduction at the surface of zero-valent metals and bimetallic systems has emerged as an important approach to the in-situ remediation of ground water. Reduction by iron metal was studied in batch and column systems to develop a mechanistic understanding of the reaction chemistry and to determine the factors that affect dechlorination rate and long term performance in field applications.

1996-10-01

478

Radon concentration measurements in the soil  

International Nuclear Information System (INIS)

Radon concentration measurement in the ground can be used for the prospecting for uranium and earthquake prediction. Some results of radon concentration measurement in the soil are presented here. The moisture condensation at the detector surface can affect on the detection efficiency. Due to this problem we tested a few filter papers on water permeability. The ratio of track densities on solid state nuclear track detector (SSNTD) in the open and the closed diffusion chamber is also determined. (author)

479

Pitting corrosion  

International Nuclear Information System (INIS)

Pitting corrosion is a kind of electrolytic corrosion by which the surface of a material is locally affected owing to inhomogeneities on the part of the material or medium. The paper deals briefly with questions relating to the importance, to parameters medium or materialwise, influence on production and construction, as well as the general conditions for pitting corrosion. In particular oxygen corrosion in unalloyed and low-alloy steel, and pitting corrosion in ferritic chromium-steel and austenitic chromium-nickel (molybdenum) steel is described. (DG).

480

Nuclear power plants and the ''dying forests''  

International Nuclear Information System (INIS)

Possible causes of forest damage are discussed. Mapping of affected areas by various workers resulted in dissimilar pictures. Release of radioactive rare gases, aerosols, tritium and carbon-14 seems unlikely to have a significant influence on forest damage. Influence of radioactive fallout, ozone formation by high-voltage transmission lines and synergistic effects is also found to be negligible.

1985-01-01

481

Neutron irradiation effects on plasma facing materials  

Energy Technology Data Exchange (ETDEWEB)

This paper reviews the effects of neutron irradiation on thermal and mechanical properties and bulk tritium retention of armour materials (beryllium, tungsten and carbon). For each material, the main properties affected by neutron irradiation are described and the specific tests of neutron irradiated armour materials under thermal shock and disruption conditions are summarized. Based on current knowledge, the expected thermal and structural performance of neutron irradiated armour materials in the ITER plasma facing components are analysed.

2000-12-01

482

Moving Towards Inclusive Education Policies and Practices? Basic Education for AIDS Orphans and Other Vulnerable Children in Zambia  

Science.gov (United States)

The global spread of HIV and AIDS has presented a major threat to development, affecting the health of the poor and many aspects of social and economic development. The greatest impact of the epidemic has been felt in sub-Saharan Africa, and Zambia ranks among the worst hit countries. The Free Basic Education Policy in Zambia upholds the right of all children to a universal basic education. This study explored staff and student perceptions of the impact of the epidemic on access to, and the quality of, basic education for AIDS-affected children, orphans and other vulnerable children (OVCs) in the Copperbelt Province of Zambia, where the HIV/AIDS prevalence rate ranges from 34 to 40%, and life expectancy has dropped to 33 years. Data were collected from education personnel in six districts of the Copperbelt with the highest prevalence of HIV and AIDS and from staff and students in six schools. The data indicated a range of factors that adversely ...

2007-07-01

483

Molecular mechanisms of the epithelial transport of toxic metal ions, particularly mercury, cadmium, lead, arsenic, zinc, and copper. Progress report, January 1, 1979-December 31, 1979  

International Nuclear Information System (INIS)

The mechanism of lead transport is presented, and especially the particular similarities or dissimilarities between lead and calcium in this process. The absorption of these metals was determined cockerels, raised on a commercial diet or on a specified diet, using in vivo ligated loop procedure. The dose administered into the loop contained 0.5 #mu#Ci "2"0"3Pb (and/or 0.1 #mu#Ci "4"7Ca), and 0.01 mM lead acetate (and/or mM CaCl_2) in 0.5 ml 0.15 M NaCl,pH 6.5. It was shown that lead is rapidly taken up by the mucosal tissue, and slowly transferred into the body, whereas less calcium is retained by the tissue and the transfer of calcium is many times as effective as that for lead. They appear to respond in a similar manner to a low calcium intake and vitamin D treatment. Increasing luminal stable lead concentration significantly reduced the percentage of radiolead significantly reduced the percentage of radiolead absorbed, but did not affect the absorption of ...

484

Method of defining features on materials with a femtosecond laser  

Energy Technology Data Exchange (ETDEWEB)

The invention relates to a pulsed laser ablation method of metals and/or dielectric films from the surface of a wafer, printed circuit board or a hybrid substrate. By utilizing a high-energy ultra-short pulses of laser light, such a method can be used to manufacture electronic circuits and/or electro-mechanical assemblies without affecting the material adjacent to the ablation zone.

2006-05-23

485

Higher harmonics of spontaneous radiation of ultrarelativistic channeled particles  

International Nuclear Information System (INIS)

The case of spontaneous radiation of channeled ultrarelativistic particles is considered when the dipolarity condition is not satisfied. The change of the particle longitudinal velocity affecting the maximum radiation frequency is included. The angular and frequency characteristics of the radiation for superhigh energies are studied for the first time. It is shown that there is an optimum energy at which the radiation density is maximum. The influence of the angle at which electrons enter a crystal and of the beam divergence on the radiation is investigated. The problem of quasichanneled particle radiation and also the radiation in axis-plane transitions are considered. (author).

1980-06-01

486

FGD performance hinges on mist-eliminator design  

Energy Technology Data Exchange (ETDEWEB)

This article examines the impact of mist-eliminator (ME) design and reliability on the performance of flue gas desulfurization (FGD) processes. ME failures are the second most common cause of FGD system outages. ME design also affects the chemistry of the FGD processes. The author considers the ME the linchpin of the whole FGD process system.

1994-07-01

487

Entrepreneurship in macroeconomics  

British Library Electronic Table of Contents (United Kingdom)

This paper reviews the main studies on entrepreneurship conducted during the past two decades that are relevant for the understanding of various macroeconomic issues. I organize the discussion by distinguishing three groups of contributions. The first group includes studies whose main purpose is to understand the factors that affect the decision to become an entrepreneur. The second group includes studies that look at the aggregate and distributional implications of entrepreneurship for savings and investment. The third group deals with issues of economic development and growth.

2009-01-01

488

Effects of acid mine drainage on a headwater stream ecosystem in Colorado  

International Nuclear Information System (INIS)

The ecological effects of acid mine drainage were investigated during the summer of 1993 on St. Kevin Gulch, a headwater stream near Leadville, Colorado. The stream currently receives acidic water from an abandoned mine. The pH downstream of the mine is between 3.5 and 4.5, and several metals exceed concentrations toxic to aquatic organisms. Zinc is present at especially high concentrations (1 to 10 mg/L) Furthermore, the stream bottom is covered with a thick layer of iron hydroxide precipitates. Effects on stream biota have been dramatic. Aquatic flora in the affected reach is limited to a green filamentous alga, Ulothrix subtilissima. Macroinvertebrate densities are significantly lower in the affected reach (mean = 99 indiv/m"2; SD = 88 indiv/M"2) compared to an upstream (pristine) reference reach (mean = 1,735 indiv/m"2; SD = 652 indiv/M"2). Functional processes were also studied in the stream. Net primary production (NPP) was measured ...

489

Effect of wind turbine array establishment at the Overgaard manor on whooper swan; Effekten paa sangsvane ved etablering af en vindmoellepark ved Overgaard gods  

Energy Technology Data Exchange (ETDEWEB)

Whooper swans habitat in the coastal regions of Denmark can be affected by wind turbine arrays on the coast. The planned array consists of 55 wind turbines very close to the swan breeding area. A possibility of moving the swans away from the windmill area would be to spread the preferred swan feeds (rape, potatoes) outside their present breeding ground. (EG) 14 refs.

1998-06-01

490

Effect of polychlorinated biphenyls (Aroclor 1254) on rhythmic pituitary-adrenal function. [Rats  

Energy Technology Data Exchange (ETDEWEB)

Numerous studies have documented the general toxicity of the polychlorinated biphenyls (PCBs) and a sizeable literature has accumulated concerning the toxic effects of PCBs on a variety of homeostatic systems. However, current information regarding the effects of PCBs on rhythmic endocrine function is quite limited. Thus, the present study was undertaken to determine whether circadian periodicity in pituitary-adrenal function is affected by exposure to PCBs.

1983-09-01

491

Disagreements between central clinical events committee and site investigator assessments of myocardial infarction endpoints in an international clinical trial: review of the PURSUIT study  

UK PubMed Central (United Kingdom)

BackgroundLimited information has been published regarding how specific processes for event adjudication can affect event rates in trials. We reviewed nonfatal myocardial infarctions...Full Text Available

2001-01-01

492

Differential decay of parent-of-origin-specific genomic sharing in cystic fibrosis-affected sib pairs maps a paternally imprinted locus to 7q34  

UK PubMed Central (United Kingdom)

Cystic fibrosis (CF) is a monogenic disease characterized by a high variability of disease severity and outcome that points to the role of environmental factors and modulating genes that shape the course...Full Text Available

2010-05-01

493

Deletion of Genes Implicated in Protecting the Integrity of Male Germ Cells Has Differential Effects on the Incidence of DNA Breaks and Germ Cell Loss  

UK PubMed Central (United Kingdom)

BackgroundInfertility affects ∼20% of couples in Europe and in 50% of cases the problem lies with the male partner. The impact of damaged DNA originating...Full Text Available

494

Cyclopeptide alkaloids. Synthesis of the ring system and its ion affinity  

International Nuclear Information System (INIS)

Several examples of the 14-membered, para-bridged ring system of the cyclopeptide alkaloids have been synthesized via an active ester cyclization. The yield of monomeric cyclopeptide varied from 1 to 33% and was affected by the amino acid substitution pattern and amide conformation of the linear peptide precursors. Both the synthetic models and a naturally occurring cyclopeptide alkaloid, ceanothine B, bind monovalent (Li"+) and divalent (Ca"2"+, Mg"2"+) cations. 4 figures, 1 table.

495

Coastal metabolism and the oceanic organic carbon balance  

Energy Technology Data Exchange (ETDEWEB)

The metabolism of organic matter in the coastal regions of the ocean may significantly affect the oceanic carbon budget. This paper describes the high percentage (30%) of oceanic oxidation that occurs in coastal areas and the impact of this metabolism on the carbon cycle and climate of earth. Organic metabolism in the ocean appears to be a source of carbon dioxide release into the atmosphere. Oxidation in the coastal zone is of special interest, as it is likely influenced by anthropogenic activity. Recommendations for future research on this topic are proposed. 129 refs., 2 figs., 6 tabs.

1993-02-01

496

Big bang nucleosynthesis and finite temperature field theory  

Energy Technology Data Exchange (ETDEWEB)

We consider electromagnetic corrections at finite temperature and their effect on the nucleosynthesis in the standard Big Bang scenario. This requires discussing the finite, temperature dependent correction to the neutron-proton mass difference as well as making use of a previous result on the temperature correction to the mass of the electron. We find that these corrections do not affect the conventional results of e.g. the helium abundance to any appreciable extent.

1982-11-11

497

Ambient water-quality criteria for ammonia (salt water)-1989  

Energy Technology Data Exchange (ETDEWEB)

Ammonia is a common and highly toxic pollutant which, in sufficient quantities, will adversely affect aquatic organisms. This ammonia criteria document for salt water will allow establishment of regulatory standards for ammonia discharge into estuaries, near coastal zones and oceans. Possible adverse impacts in highly sensitive and abundant ecosystems such as estuaries make regulation particularly important.

1989-04-01

498

Administration of a Decoction of Sucrose- and Polysaccharide-Rich Radix Astragali (Huang Qi) Ameliorated Insulin Resistance and Fatty Liver but Affected Beta-Cell Function in Type 2 Diabetic Rats  

UK PubMed Central (United Kingdom)

The current investigation attempted to confirm the beneficial actions of a chemically characterized Radix Astragali decoction (AM-W) against type 2 diabetic (T2D) Sprague-Dawley (SD) rats. Using a case/control...Full Text Available

2011-01-01

499

A numerical weather prediction model--Computational aspects on the CRAY-1  

Science.gov (United States)

This paper presents a case study in the design and implementation of a numerical weather prediction model on a supercomputer (CRAY-1). Following a historical introduction to the evolution of the model, the governing equations of the model are presented and the numerical solution of these forecast equations is described. A brief tutorial on the architecture of the CRAY-1 is presented with a discussion of how it affects the choice of algorithms and code design of the model. A summary of the advantages gained by use of the vector aspects of the CRAY-1 is included.

1984-01-01

500

A comparison of nutrient dynamics in forest ecosystems along with the Warmth Index Gradient  

Energy Technology Data Exchange (ETDEWEB)

Nutrient elements contained in litter fall flux, that of uptake flux and turnover rate had generally tended to increase with the increase in the Warmth Index, while the amount of nutrient in the A[sub 0] horizon and nutrient use efficiency did not. However, it is suggested that topographic and climatic aridity, and the amount of available and exchangeable phosphorus, calcium and magnesium greatly affect the nutrient dynamics in a each forest ecosystem as the Warmth Index increases. (J.P.N.).

1993-01-01