a-rich upstream sequence: Topics by WorldWideScience.org

Molecular cloning, genomic organization, and chromosomal localization of the human pancreatitis-associated protein (PAP) gene

Peculiar DNA sequences made up by the tandem repetition of a 5 bp unit have been identified within or upstream from three avian protein-coding genes. One sequence is located within an intron of the...Full Text Available

1983-03-11

3

Solidification of radioactive waste effluents

Pancreatitis-associated protein (PAP) is a secretory pancreatic protein present in small amounts in normal pancreas and overexpressed during the acute phase of pancreatitis. In this paper, the authors describe the cloning, characterization, and chromosomal mapping of the human PAP gene. The gene spans 2748 bp and contains six exons interrupted by five introns. The gene has a typical promoter containing the sequences TATAAA and CCAAT 28 and 52 bp upstream of the cap site, respectively. They found striking similarities in genomic organization as well as in the promoter sequences between the human and rat PAP genes. The human PAP gene was mapped to chromosome 2p12 using rodent-human hybrid cells and in situ chromosomal hybridization. This localization coincides with that of the reg/lithostathine gene, which encodes a pancreatic secretory protein structurally related to PAP, suggesting that both genes derived from the same ...

1994-01-01

4

Polymorphisms in the p53 gene in thyroid tumours and blood samples of children from areas in Belarus

A process and apparatus for solidifying radioactive waste liquid containing dissolved and/or suspended solids is disclosed. The process includes chemically treating for pH adjustement and precipitation of solids, concentrating solids with a thin-film evaporator to provide liquid concentrate containing about 50% solids, and drying the concentrate with heated mixing apparatus. The heated mixing apparatus includes a heated wall and working means for shearing dried concentrate from internal surfaces and subdividing dry concentrate into dry, powdery particles. The working means includes a rotor and helical means for positively advancing the concentrate and resulting dry particles from inlet to outlet of the mixing apparatus. The dry particles may also be encapsulated in a matrix material. Entrained particles in the vapor stream from the evaporator and mixer are removed in an integral particle separator and the vapor is subsequently condensed and may be recycled upstream ...

1983-10-11

5

NADP Regulates the Yeast GAL Induction System

We present changes in the p53 gene in a group of 70 thyroid tumours and 40 blood samples obtained from children from Belarus. Three thyroid tumours show a polymorphism in exon 6 (codon 213) and 5 tumours show a polymorphism in intron 6, 37 bp upstream to the 5'-end of exon 7. Only one patient has a mutation in exon 7 (codon 258) resulting in an amino acid substitution in the protein p53. The distribution of polymorphisms in the 40 blood samples was as follows: three patients had a polymorphism in exon 6 and two persons had a polymorphism in intron 6. One polymorphism in intron 6 was also found in the group of 30 healthy children from Belarus. The fact that the differences in the sequence in p53 found in the tumours was also seen in the blood of these patients demonstrates that they are polymorphisms not induced by radiation exposure. It is difficult to conclude, if the polymorphisms found by us could be associated with the predisposition to ...

6

Analysis of the 5{prime} region of PMS2 reveals heterogeneous transcripts and a novel overlapping gene

Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UASGAL); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5'-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.

2008-01-01

7

Apparatus for measuring the decontamination factor of a multiple filter air-cleaning system

The PMS2 gene encodes a protein that is involved in DNA mismatch repair and is mutated in a subset of patients with hereditary nonpolyposis colon cancer (HNPCC). The previously published PMS2 cDNA sequence lack an upstream in-frame stop codon preceding the presumptive initiating methionine. To evaluate the 5` terminus of the PMS2 coding region further, we isolated additional cDNA clones, RT-PCR products, and the corresponding 5` genomic segment of the PMS2 locus. The PMS2 gene transcripts were found to have heterogeneous but colinear 5` termini, one of which contained an in-frame termination codon preceding the initiating methionine. In addition, a novel gene encoding a 34.5-kDa polypeptide was found to initiate transcriptionally within PMS2 from the opposite strand. 23 refs., 5 figs., 2 tabs.

1995-09-20

8

Molecular basis of FIR-mediated c-myc transcriptional control

An apparatus is described for measuring the overall decontamination factor of first and second filters, the first and second filters being located in a plenum having an upstream chamber, an intermediate chamber, and a downstream chamber. The first filter is located between the upstream and intermediate chambers and the second filter is located between the intermediate and downstream chambers. The apparatus comprises a. an aerosol generator for producing a challenge aerosol composed of individual particles, the aerosol generator being in fluid communication with the upstream chamber; b. an upstream collector for collecting the challenge aerosol before the challenge aerosol enters the first filter, the upstream collector being disposed in the upstream chamber; c. an intermediate collector for collecting the challenge aerosol that passes through the first filter, ...

1986-10-28

9

Source tracking of Escherichia coli by 16S-23S intergenic spacer region denaturing gradient gel electrophoresis (DGGE) of the rrnB ribosomal operon.

The Far UpStream Element (FUSE) regulatory system promotes a peak in the concentration of c-Myc during cell cycle. First, the FBP transcriptional activator binds to the FUSE DNA element upstream...Full Text Available

2010-09-01

10

Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase

This research validates a novel approach for source tracking based on denaturing gradient gel electrophoresis (DGGE) analysis of DNA extracted from Escherichia coli isolates. Escherichia coli from different animal sources and from river samples upstream from, at, and downstream of a combined sewer overflow were subjected to DGGE to determine sequence variations within the 16S-23S intergenic spacer region (ISR) of the rrnB ribosomal operon. The ISR was analyzed to determine if E. coli isolates from various animal sources could be differentiated from each other. DNA isolated from the E. coli animal sources was PCR amplified to isolate the rrnB operon. To prevent amplification of all 7 E. coli ribosomal operons by PCR amplification using universal primers, sequence-specific primers were utilized for the rrnB operon. Another primer set was then used to prepare samples of the 16S-23S ISR for DGGE. Comparison of PCR-DGGE results ...

2007-10-01

11

By catalyzing the rate-limiting step in adipose tissue lipolysis, hormone-sensitive lipase (HSL) is an important regulator of energy homeostasis. The role and importance of HSL in tissues other than adipose are poorly understood. We report here the cloning and expression of a testicular isoform, designated HSL{sub tes}. Due to an addition of amino acids at the NH{sub 2}-termini, rat and human HSL{sub tes} consist of 1068 and 1076 amino acids, respectively, compared to the 768 and 775 amino acids, respectively, of the adipocyte isoform (HSL{sub adi}). A novel exon of 1.2 kb, encoding the human testis-specific amino acids, was isolated and mapped to the HSL gene, 16 kb upstream of the exons encoding HSL{sub adi}. The transcribed mRNA of 3.9 kb was specifically expressed in testis. No significant similarity with other known proteins was found for the testis-specific sequence. The amino acid composition differs from the HSL{sub adi} ...

1996-08-01

12

Hydrobia schoutedeni

... River at Ango Ango (about 4 km from Matadi (probably upstream)), and the second at Chaundron de ... proposal were to be adopted the site at Matadi would be lost. No conservation measures in ...

13

Hydrobia plena

Molecular events involved in ionizing radiation induced skin carcinogenesis

... River at Ango Ango (4 km south of Matadi (upstream)), and the second on a small tributary ... proposal were to be adopted the site at Matadi would be lost. No conservation measures in place ...

14

Increased expression after x-irradiation of MUC1 in cultured human colon carcinoma HT-29 cells

The process of mouse skin tumor formation is subdivided into three operational stages. These stages include initiation, promotion and progression. Ionizing radiation has been found to be a weak initiating agent in the production of malignant squamous cell carcinomas, a complete carcinogen and an agent effective in causing tumor progression. Four skin tumor histologies have been seen with ionizing radiation: benign papillomas, squamous (SCC) and basal (BCC) cell carcinomas and fibrosarcomas. Distinct non-ras transforming genes have been detected in radiation initiated SCCs. A benign papilloma cell line (308) was used as a model system to study ionizing radiation induced progression. A variant 308 cell line (308 10 Gy 5) derived by irradiation of the parental 308 cell has been characterized. The 308 10 Gy 5 cells unlike the parental 308 cells from malignant tumors in athymic nude mice upon subcutaneous injection. The variant 308 10 Gy 5 cells unlike the parental cells also show by ...

15

An enhancer element is located 340 base pairs upstream from the adenovirus-2 E1A capsite.

The effect of X-irradiation on production of MUC1 was studied with human colon carcinoma HT-29 cells. As evaluated by immunocytochemical staining, the percentages or MUC1-positive cells in cells at 4 days after 6 Gy irradiation and in unirradiated control cells were 52#+-#3.5% (n=6) and 26#+-#2.8% (n=6), respectively. Flow-cytometric analysis of living cells showed that MUC1 began to rise from day 1, reaching a plateau by day 4 after 6 Gy irradiation. Western blot analysis with monoclonal antibody MY.1E12 against glycosylated MUC1 (mature form) showed dose-dependent increases of two bands (500 and 390 kDa) corresponding to two polymorphic MUC1 alleles. Premature forms of MUC1 (350 and 240 kDa) were detectable with monoclonal antibody HMFG-2 only in irradiated cells, suggesting that new core protein synthesis had been induced. The transcriptional activity of the MUC1 gene was analyzed in terms of transient expression of MUC1-CAT reporter plasmids containing 5'-flanking ...

2000-03-01

16

The importance of an indigenous tree to southern African communities with specific relevance to its domestication and commercialization: a case of the marula tree

A chimeric recombinant, containing the 270 bp left-terminal fragment of Adenovirus-2 (Ad2) inserted upstream from the -34 to +33 Ad2 major late promoter (Ad2MLP) element, has been used to characterize...Full Text Available

1983-12-20

17

British Library Electronic Table of Contents (United Kingdom)

Local communities in southern Africa derive many benefits from marula [Sclerocarya birrea (A. Rich.) Hochst. subsp. caffra (Sond.) Kokwaro]. These include the contribution of this species towards health, nutrition, food security and conservation by sharing local skills and knowledge related to it. Marula fruits can be eaten fresh, squeezed to make juice, brewed in traditional beer or used to make jam and jelly. The kernels are also edible and can be pressed to extract oil for cooking and cosmetics, i.e., for skin and hair application. The bark, roots, seeds and leaves are exploited for traditional medicinal purposes. Marula has acquired significant commercial value since its fruits and other products have entered local, regional and international trade in southern Africa. To diversify frui...

2011-01-01

18

Isotopes and groundwater management strategies under semi-arid area: Case of the Souss upstream basin (Morocco)

Device for energy-saving heating of fuel in the fuel supply for an internal combustion engine

This study concerns the Souss upstream basin. The objective is to investigate the characteristics of surface water and groundwater, to assess the impact of artificial recharge as reinforcement of the natural replenishment and assess the renewal of groundwater under semi-arid area. Two major water types are observed: (i) surface waters and upstream springs (least mineralized) and (ii) all groundwater samples (prevailing calcium and magnesium bicarbonate water type). Water isotopes show a low evaporation of precipitations during infiltration. Impoverishment in heavy isotopes is the characteristic of mountain rainfalls, or of a climate colder and wetter than present. Carbon-14 activities (34-94 pmc) indicate a long residence time. The artificial recharge is low compared to the reservoir volume, due to which the renewal rate is also low.

2011-07-01

19

Interactive computer programs in sequence data analysis.

The invention concerns a device for the energy-saving heating of fuel in the supply pipe to an internal combustion engine to regain energy from the fuel itself, particularly but not exclusively for a Diesel engine. A part of the quantity of heat added to the fuel between the tank and the injection nozzles is given up by excess fuel not to the tank, but to the fuel lifted upstream of the injection pump. The device is characterised by the fact that it has a heat exchanger, which absorbs heat from the fuel at the level of the injection pump or upstream of it, and takes this to the fuel above the injection pump. The heat exchanger is preferably mounted upstream of a filter and close to it. A bridging pipe, which may be integrated in the heat exchanger, permits the heat exchanger to be short-circuited depending on the fuel temperature.

1981-02-10

20

Effects of Mitigative Measures on Productivity of White Sturgeon Populations in the Columbia River Downstream from McNary Dam, and Status and Habitat Requirements of White Sturgeon Populations in the Columbia and Snake Rivers Upstream from McNary Dam, 1993-1994 Annual Report.

We present interactive computer programs for the analysis of nucleic acid sequences. In order to handle these programs, minimum computer experience is sufficient. The nucleotide sequence of the human...Full Text Available

1982-01-11

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21

Downstream natural gas in Europe-High hopes dashed for upstream oil and gas companies

No abstract prepared.

1995-05-01

22

Multilocus Sequence Analysis and rpoB Sequencing of Mycobacterium abscessus (Sensu Lato) Strains?

Access for independents to retail gas markets was a central concern in European policy reform efforts in the 1990s. Upstream oil and gas companies reacted with strategic intentions of forward integration. By late 2004, forward integration was still weak, however. An important explanation of the gap between announced strategic re-orientation and actual strategy implementation lies in the political failure of EU member states to dismantle market barriers to entry for independents. Variations between companies in downstream strategy implementation are explained by variations in business opportunities and internal company factors.

2007-01-01

23

Control effects of the flow and the aerodynamic force around the downstream cylinder by a spinning upstream cylinder in uniform flow

Mycobacterium abscessus, Mycobacterium bolletii, and Mycobacterium...Full Text Available

2011-02-01

24

Canadian upstream oil and gas industry fire and explosion incident analysis based on the investigative work of the IRP18 Committee working with the University of Calgary Department of Chemical and Petroleum Engineering. Revision 1

The aerodynamic forces and wake structure of the non-rotating downstream circular cylinder, of which the uniform free stream flow is interfered with another spinning upstream cylinder having the same diameter that is located upstream in a line have been investigated experimentally. When the spin rate of the downstream cylinder defined as the ratio of tangential surface velocity of the spinning cylinder to the free stream velocity increases gradually from zero to 1.4, the change of surface pressure distribution, aerodynamic forces of the non-rotating downstream cylinder were measured in case of several distance ratios of 1.5, 3.0, and 4.5 defined as the ratio of distance between the centers of two cylinders to the diameter. The wake flow patterns behind the cylinder were also investigated in each case. From the present experiments, it has been found that the spin rate significantly influences the aerodynamic forces and near-wake flow phenomena ...

1998-03-01

25

Protein Coding Sequence Identification by Simultaneously Characterizing the Periodic and Random Features of DNA Sequences

This report presented an analysis of incidents gathered by the Canadian upstream oil and gas industry committee in relation to explosive atmospheres in wellbores, vessels, tanks, and piping systems. The aim of the report was to develop industry recommended practices for oil and gas industry fires and explosions. Two accident theory models were used to set out the relationships between hazards, defenses, and losses. Three levels of defenses were identified based on organizational, local workplace, and human factors. An organizational responsibility approach was used to examine the activities of key people with the system. Incidents were analyzed based on an expanded fire triangle used to identify fire and explosion hazards. The study showed that the ignition of hydrocarbons into the air was a factor in nearly 50 per cent of the incidents. A lack of understanding of liquid-hydrocarbon properties was demonstrated in incidents involving oxidized hydrocarbons. A lack of ...

2005-05-15

26

Los Alamos sequence analysis package for nucleic acids and proteins.

Most codon indices used today are based on highly biased nonrandom usage of codons in coding regions. The background of a coding or noncoding DNA sequence, however, is fairly random, and can be characterized...Full Text Available

2005-01-01

27

High-density multiplex detection of nucleic acid sequences: oligonucleotide ligation assay and sequence-coded separation.

An interactive system for computer analysis of nucleic acid and protein sequences has been developed for the Los Alamos DNA Sequence Database. It provides a convenient way to search or verify various...Full Text Available

1982-01-11

28

DNA sequencing using biotinylated dideoxynucleotides and mass spectrometry

We describe a non-isotopic, semi-automated method for large-scale multiplex analysis of nucleic acid sequences, using the cystic fibrosis transmembrane regulator (CFTR) gene as an example. Products...Full Text Available

1994-10-25

29

Actin-like sequences are present on human X and Y chromosomes.

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MS) has been explored widely for DNA sequencing. The major requirement for this method is that the DNA sequencing fragments...Full Text Available

2001-11-01

30

Investigation of the unsteadiness of a shock-reflection interaction with time-resolved particle image velocimetry

The human genome contains greater than 20 actin-related sequences, six of which at least are expressed as protein. We have shown by blot hybridization the presence of actin-like sequences on both the...Full Text Available

1984-08-01

31

British Library Electronic Table of Contents (United Kingdom)

The spatio-temporal dynamics of an impinging shock/boundary layer interaction at Mach 2 and under incipient separation conditions, has been investigated experimentally by means of high-speed particle image velocimetry (PIV). The available PIV acquisition rate of up to 20 kHz permits a time-resolved characterization of the interaction. The dynamics of different flow regions?notably the separation region and the reflected shock?were quantified by means of temporal auto-correlation fields and pseudo-spectral analysis. The PIV data further enable to investigate the relationship between spatially extended flow features, such as shock position and bubble size, as well as the influence of the upstream boundary layer. The results confirm earlier studies that there is an important upstream effect o...

2011-01-01

32

Apparatus for measuring the decontamination factor of a multiple filter air-cleaning system

Time-dependent ARMA modeling of genomic sequences

An apparatus for measuring the overall decontamination factors of first and second filters located in a plenum. The first filter separates the plenum's upstream and intermediate chambers. The second filter separates the plenum's intermediate and downstream chambers. The apparatus comprises an aerosol generator that generates a challenge aerosol. An upstream collector collects unfiltered aerosol which is piped to first and second dilution stages and then to a laser aerosol spectrometer. An intermediate collector collects challenge aerosol that penetrates the first filter. The filtered aerosol is piped to the first dilution stage, diluted, and then piped to the laser aerosol spectrometer which detects single particles. A downstream collector collects challenge aerosol that penetrates both filters. The twice-filtered aerosol is piped to the aerosol spectrometer. A pump and several valves control the movement of aerosol within the ...

1985-07-03

33

Stellar evolution. II - The evolution of a 3 sun-mass star from the main sequence through core helium burning.

BackgroundOver the past decade, many investigators have used sophisticated time series tools for the analysis of genomic sequences. Specifically, the correlation of the nucleotide...Full Text Available

34

Genome analysis with inter-nucleotide distances

Three Sun-mass star evolution from main sequence to helium exhaustion in core, noting chronology of

1965-01-01

35

Compressing DNA sequence databases with coil

Motivation: DNA sequences can be represented by sequences of four symbols, but it is often useful to convert the symbols into real or complex numbers for further analysis. Several mapping...Full Text Available

2009-12-01

36

NO formation in the burnout region of a partially premixed methane-air flame with upstream heat loss

BackgroundPublicly available DNA sequence databases such as GenBank are large, and are growing at an exponential rate. The sheer volume of data being dealt with presents serious...Full Text Available

37

Heat transfer augmentation in rod bundles near grid spacers

Measurements of temperature and NO concentration in laminar, partially premixed methane-air flames stabilized on a ceramic burner in coflow are reported. The NO concentration and temperature were determined by laser-induced fluorescence (LIF) and coherent anti-Stokes Raman scattering (CARS), respectively. Upstream heat loss to the burner was varied by changing the exit velocity of the fuel-air mixture at a constant equivalence ratio of 1,3; this alters the structure of the flame from an axisymmetric Bunsen-type to a strongly stabilized flat flame. To facilitate analysis of the results, a method is derived for separating the effects of dilution from those of chemical reaction based on the relation between the measured temperature and the local mixture fraction, including the effects of upstream heat loss. Using this method, the amount of NO formed during burnout of the hot, fuel-rich combustion products can be ascertained. In the Bunsen-type ...

1999-09-01

38

Combined gas/steam turbine process. Kombinierter Gas/Dampfturbinen-Prozess

Heat transfer augmentation by straight grid spacers in rod bundles is studied for single phase flow and for post critical heat flux dispersed flow. The heat transfer effect of swirling grid spacers in single phase flow is also examined. Governing heat transfer mechanisms are analyzed, and predictive formulations are established. For single phase flow, the local heat transfer at a straight spacer and at its upstream or downstream locations are treated separately. 18 refs.

1980-01-01

39

A photon beam position monitor for SSRL beamline 9

A combined gas/steam turbine process includes a high-pressure furnace and a high pressure gasification unit with a mounted upstream of a combustion chamber there is a gas turbine with a waste heat system. Combustion heat which is not needed to heat the flue gas to combustion temperature is released from the furnace and transferred to ths combustion air going into the combustion chamber.

1991-01-31

40

{sup 13}C-NMR signal enhancement by using parahydrogen induced polarization (PGIP) and appropriate pulse sequences

We present here the concept of a simple one dimensional photon beam position monitor for use with high power synchrotron radiation beams. It has micron resolution, reasonable linearity in an inexpensive design. Most important, is its insensitivity to diffusely scattered low energy radiation from components upstream of the monitor.

1995-10-01

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41

Secondary Structure Analysis of a G-rich Sequence Recognizing RNA Aptamer with Structure Specific Enzymes

No abstract prepared.

2009-07-01

42

Congenital Inner Ear Malformation: Three Dimensional Volume Rendering Image Using MR CISS Sequence

Full Text Available

2011-06-01

43

CloVR: A virtual machine for automated and portable sequence analysis from the desktop using cloud computing.

Full Text Available

2003-10-01

44

2009 Community Sequencing Program: Life Under Ice

PMID:21878105

2011-08-30

45

ScienceCinema

...carbon dioxide in the atmosphere contributes to global warming anything that could ...

46

Assembly sequencing with toleranced parts

White River Falls Fish Passage Project, Tygh Valley, Oregon : Final Technical Report, Volume III, Appendix B, Fisheries Report; Appendix C, Engineering Alternative Evaluation; Appendix D, Benefit/Cost Analysis.

The goal of assembly sequencing is to plan a feasible series of operations to construct a product from its individual parts. Previous research has thoroughly investigated assembly sequencing under the assumption that parts have nominal geometry. This paper considers the case where parts have toleranced geometry. Its main contribution is an efficient procedure that decides if a product admits an assembly sequence with infinite translations that is feasible for all possible instances of the components within the specified tolerances. If the product admits one such sequence, the procedure can also generate it. For the cases where there exists no such assembly sequence, another procedure is proposed which generates assembly sequences that are feasible only for some values of the toleranced dimensions. If this procedure produces no such sequence, ...

1995-02-21

47

Heat shock factor binds to heat shock elements upstream of heat shock protein 70a and Samui genes to confer transcriptional activity in Bombyx mori diapause eggs exposed to 5^oC

Studies were conducted to describe current habitat conditions in the White River basin above White River Falls and to evaluate the potential to produce anadromous fish. An inventory of spawning and rearing habitats, irrigation diversions, and enhancement opportunities for anadromous fish in the White River drainage was conducted. Survival of juvenile fish at White River Falls was estimated by releasing juvenile chinook and steelhead above the falls during high and low flow periods and recapturing them below the falls in 1983 and 1984. Four alternatives to provide upstream passage for adult salmon and steelhead were developd to a predesign level. The cost of adult passage and the estimated run size of anadromous fish were used to determine the benefit/cost of the preferred alternative. Possible effects of the introduction of anadromous fish on resident fish and on nearby Oak Springs Hatchery were evaluated. This included an inventory of resident species, a genetic ...

1985-06-01

48

British Library Electronic Table of Contents (United Kingdom)

To understand the molecular mechanisms of how 5^oC-incubation activates mRNA expression of Hsp70a and Samui genes in Bombyx mori diapause eggs, we first searched the 5'-upstream regions of the Hsp70a and Samui genes for heat shock elements (HSEs) and found two regions [Hsp70aHSE-1 (-95 to -58) and -2 (-145 to -121), and SamuiHSE-1 (-84 to -55) and -2 (-304 to -290)] corresponding to HSEs (repeats of nGAAn and/or nTTCn). We cloned four cDNAs encoding heat shock factor (HSF)-a2 (627 amino acids), -b (685 aa), -c (682 aa) and -d (705 aa), which were produced by alternative splicing. When we exposed diapause eggs to 5^oC beginning at 2 day post-oviposition to break diapause, HSFd mRNA only increased after chilling for 6-8 days, a pattern very similar to those of Hsp70a and Samui mRNAs. To exam...

2011-01-01

49

Evaluation of performance and characteristics of neutron radiography and standardization

Conceptual design of a hydrogen production system by DME steam reforming and high-efficiency nuclear reactor technology

Neutron radiography has been put to practical use in foreign countries as one of nondestructive inspection technologies, but in Japan, its application to other scientific fields is mainly researched. The reason is, the neutron radiography apparatuses installed in Japan are hard to be industrially utilized for a number of reasons, but also the characteristics of neutron radiography are not clearly established, and it is not standardized. The part from a collimator through an object to a detector is called ``upstream``, the part of an image detector is called ``midstream``, and the part of image processing is called ``downstream``. The nonparallel property of beam, image distortion and the lack of sharpness, beam quality and the interaction of neutron beam with an object are discussed about the upstream. The intensity of neutron beam, the energy spectra, the effective total macroscopic cross section, the effective energy, the evaluation of ...

1995-02-01

50

Reducing the breakdowns and energy consumption in pressure regulating stations by rationalizing the heating of gas

Hydrogen is a potential alternative energy source and produced commercially by methane (natural gas) or LPG steam reforming, a process that requires high temperatures, which are produced by burning fossil fuels. However, since this process emits large amounts of CO_2, replacement of the combustion heat source with a nuclear heat source for 773-1173 K processes has been proposed in order to eliminate these CO_2 emissions. This paper proposes a novel method of low-temperature nuclear hydrogen production by reforming dimethyl ether (DME) with steam produced by a low-temperature nuclear reactor at about 573 K. The authors identified conditions that provide high hydrogen production fraction at low pressure and temperatures of about 523-573 K. By setting this low-temperature hydrogen production process at about 573K upstream from a turbine, it was found theoretically that the total energy utilization efficiency is about 50% and very high. By setting a turbine ...

2003-09-15

51

Oman: current status, upstream and export developments, investment opportunities

Sigma 1) examines ways to reduce the number of breakdowns of pressure-regulating stations and to minimize the energy consumed in warming the gas, 2) determines the optimal gas temperatures upstream from the pressure reduction, and 3) shows the dependence of energy consumption upon the water dewpoint. The method of calculating the optimal input temperature relies on Mollier's diagram. Tables and nomographs for natural gas and water dewpoint at 19/sup 0/F and 580 psi (-7/sup 0/C and 4 MPa) are useful in achieving the desired gas temperatures. Heating the regulators' control valves is important.

1982-04-01

52

Nonlinear dynamics of heat transfer enhancement using eddy promoters

Oman is an attractive and welcoming country, well-ordered and unpretentious. It has benefited from remarkable achievements of economic and social development since 1970. It now has a resource base that should be sufficient to keep it on continuing path of further development. However, the second 20-years of modern Oman will inevitable contain some testing challenges. These are centred in two main areas - the economic, where expenditure has already outstripped revenue and where firm controls are needed to maintain a reasonable balance; and the social, where the pressures from an increasingly literate and numerous population will demand changes to the autocratic traditional system of government. (author)

1995-11-01

53

Nonlinear dynamics of heat transfer enhancement using eddy promoters

This investigation concerns the nonlinear dynamics of heat transfer from a surface using an upstream eddy promoter. A numerical model is developed for the coupled fluid flow and heat transfer process based on a modified MacCormack scheme. Numerical simulations are carried out to determine the response and heat transfer enhancement due to the promoter. The average heat transfer from a cavity floor is seen to be increased by a factor of approximately five over the unpromoted'' flow. Another interesting feature of the study is the nonlinear viscous flow dynamics from the cylinder-wall interaction which differ significantly from the familiar cylinder-free stream patterns.

1994-02-01

54

Flue gas desulfurization wastewater treatment primer

This investigation concerns the nonlinear dynamics of heat transfer from a surface using an upstream eddy promoter. A numerical model is developed for the coupled fluid flow and heat transfer process based on a modified MacCormack scheme. Numerical simulations are carried out to determine the response and heat transfer enhancement due to the promoter. The average heat transfer from a cavity floor is seen to be increased by a factor of approximately five over the unpromoted'' flow. Another interesting feature of the study is the nonlinear viscous flow dynamics from the cylinder-wall interaction which differ significantly from the familiar cylinder-free stream patterns.

1994-02-01

55

Alberta oil sands royalty regime

Purge water from a typical wet flue gas desulfurization system contains myriad chemical constituents and heavy metals whose mixture is determined by the fuel source and combustion products as well as the stack gas treatment process. A well-designed water treatment system can tolerate upstream fuel and sorbent arranged in just the right order to produce wastewater acceptable for discharge. This article presents state-of-the-art technologies for treating the waste water that is generated by wet FGD systems. 11 figs., 3 tabs.

2009-03-15

56

The viroid and viroid-like RNA database.

The long term objective of the Oil Sands Business Unit of Alberta Energy is to pave the way for Alberta's bitumen production to reach 3 million barrels per day by 2020. This presentation described the national government's role in resource development. It was emphasized that since the Crown is the owner of the oil sands resource, it would benefit by providing strategic leadership and by generating a larger royalty base. The oil sands fiscal regime was described with reference to generic royalty, risk sharing, investment, and project economics. Business rule principles were also outlined along with criteria for project expansions. Both upstream and downstream challenges and opportunities were listed. 4 figs.

2004-01-29

57

The ovalbumin gene family: complete sequence and structure of the Y gene.

The viroid and viroid-like RNA database is a compilation of all natural sequences published in journals or available from the GenBank and EMBL nucleotide sequence libraries. Several information regarding...Full Text Available

1998-01-01

58

The comparative structure of mammalian glyceraldehyde 3-phosphate dehydrogenase

The "ovalbumin Y" gene, one of three which constitute the ovalbumin gene family in chicken has been completely sequenced. The exact location of exons can be derived from the comparison with the ovalbumin...Full Text Available

1982-07-24

59

Sequence space coverage, entropy of genomes and the potential to detect non-human DNA in human samples

1. The amino acid sequences around the thiol groups of glyceraldehyde 3-phosphate dehydrogenase from badger and monkey skeletal muscle were compared with the sequences around the thiol groups in the...Full Text Available

1969-01-01

60

Sequence features that drive human promoter function and tissue specificity

BackgroundGenomes store information for building and maintaining organisms. Complete sequencing of many genomes provides the opportunity to study and compare global information properties...Full Text Available

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61

SEQADAPT: an adaptable system for the tracking, storage and analysis of high throughput sequencing experiments

Promoters are important regulatory elements that contain the necessary sequence features for cells to initiate transcription. To functionally characterize a large set of human promoters, we measured...Full Text Available

2010-07-01

62

Molecular cloning, cDNA sequence, and chromosomal assignment of the human radixin gene and two dispersed pseudogenes

BackgroundHigh throughput sequencing has become an increasingly important tool for biological research. However, the existing software systems for managing and processing these data...Full Text Available

63

Molecular cloning and nucleotide sequence of the beta-lytic protease gene from Achromobacter lyticus.

Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. Recent cloning of the murine and porcine radixin cDNAs revealed a protein highly homologous to ezrin and moesin. The authors have cloned and sequenced the human radixin cDNA and found the predicted amino acid sequence for the human protein to be nearly identical to those predicted for radixin in the two other species. By Southern analyses of Chinese hamster x human somatic cell hybrid DNA and of PCR products derived from hybrids, the coding gene (RDX) was mapped to 11q. Fluorescence chromosomal in situ hybridization with a cDNA plasmid further localized this gene to band 11q23. However, PCR amplification with [open quotes]radixin-specific[close quotes] primers on the hybrid DNA panel yielded an additional, very similar DNA sequence that was further characterized by direct sequencing of PCR products. This ...

1993-04-01

64

Massive turnover of functional sequence in human and other mammalian genomes

Two bacteriolytic enzymes secreted by Achromobacter lyticus M497-1 were purified and identified as being very similar (considering their amino acid composition and N-terminal sequence) to alpha- and...Full Text Available

1990-11-01

65

Left ventricular volumetry by nuclear magnetic resonance imaging: the interest of the phased array antenna and of the segmented gradient echo sequences; Volumetrie ventriculaire gauche en IRM: interet des sequences d`echo de gradient segmentees et de l`antenne phased array

Despite the availability of dozens of animal genome sequences, two key questions remain unanswered: First, what fraction of any species' genome confers biological function, and second, are apparent...Full Text Available

2010-10-01

66

Isolation, sequence analysis, and physiological properties of enkephalins in the nervous tissue of the shore crab Carcinus maenas L.

Short communication

1995-10-01

67

Improving the Arabidopsis genome annotation using maximal transcript alignment assemblies

[Leu]- and [Met]enkephalin from thoracic ganglia of the shore crab Carcinus maenas have been purified to homogeneity by a reversed-phase HPLC procedure. Automated gas-phase sequencing revealed a primary...Full Text Available

1991-10-01

68

Improved DNA Sequencing Accuracy and Detection of Heterozygous Alleles Using Manganese Citrate and Different Fluorescent Dye Terminators

The spliced alignment of expressed sequence data to genomic sequence has proven a key tool in the comprehensive annotation of genes in eukaryotic genomes. A novel algorithm was developed to assemble...Full Text Available

2003-10-01

69

The use of dideoxynucleotide triphosphates labeled with different fluorescent dyes (dye terminators) is the most versatile method for automated DNA sequencing. However, variation in peak heights reduces...Full Text Available

1999-06-01

70

Genome Sequence and Comparative Analysis of the Solvent-Producing Bacterium Clostridium acetobutylicum

BackgroundA large fraction of the human genome is attributable to L1 retrotransposon sequences. Not only do L1s themselves make up a significant portion of the genome, but L1-encoded...Full Text Available

2003-01-01

71

Evolution of the nuclear ribosomal DNA intergenic spacer in four species of the Daphnia pulex complex

The genome sequence of the solvent-producing bacterium Clostridium acetobutylicum ATCC 824 has been determined by the shotgun approach. The genome consists of a 3.94-Mb chromosome and...Full Text Available

2001-08-01

72

Detection of Atopobium vaginae in Postmenopausal Women by Cultivation-Independent Methods Warrants Further Investigation

BackgroundConcerted evolution refers to the pattern in which copies of multigene families show high intraspecific sequence homogeneity but high interspecific sequence diversity....Full Text Available

73

Completely phased genome sequencing through chromosome sorting

We sequenced 16S rRNA genes from the vaginal swab contents of a postmenopausal woman with asymptomatic bacterial vaginosis (BV). Sequences from Atopobium vaginae were the most commonly...Full Text Available

2004-04-01

74

Complete plastid genome sequences suggest strong selection for retention of photosynthetic genes in the parasitic plant genus Cuscuta

The two haploid genome sequences that a person inherits from the two parents represent the most fundamentally useful type of genetic information for the study of heritable diseases and the development...Full Text Available

2011-01-04

75

Cellobiose dehydrogenase from Phanerochaete chrysosporium is encoded by two allelic variants.

BackgroundPlastid genome content and protein sequence are highly conserved across land plants and their closest algal relatives. Parasitic plants, which obtain some or all of their...Full Text Available

76

An excess of rare genetic variation in ABCE1 among Yorubans and African-American individuals with HIV-1

The complete nucleotide sequences of two alleles of cellobiose dehydrogenase, cdh-1 (3,627 bp) and cdh-2 (3,623 bp), from Phanerochaete chrysosporium OGC101 are reported. The nucleotide sequences of...Full Text Available

1997-02-01

77

A proposal to sequence the genome of a garter snake (Thamnophis sirtalis)

Signatures of natural selection occur throughout the human genome and can be detected at the sequence level. We have re-sequenced ABCE1, a host candidate gene essential for...Full Text Available

2009-12-01

78

A Software System for Data Analysis in Automated DNA?Sequencing

Here we develop an argument in support of sequencing a garter snake (Thamnophis sirtalis) genome, and outline a plan to accomplish this. This snake is a common, widespread, nonvenomous...Full Text Available

79

A Coxiella burnetti repeated DNA element resembling a bacterial insertion sequence.

Software for gel image analysis and base-calling in fluorescence-based sequencing consisting of two primary programs, BaseFinder and GelImager, is described. BaseFinder is a framework for trace processing,...Full Text Available

1998-06-01

80

5-Fluorodeoxyuridine as an alternative to the synthesis of mixed hybridization probes for the detection of specific gene sequences.

A DNA fragment located on the 3' side of the Coxiella burnetii htpAB operon was determined by Southern blotting to exist in approximately 19 copies in the Nine Mile I genome. The DNA sequences of this...Full Text Available

1992-09-01

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81

Use of man-made impoundment in mitigating acid mine drainage in the North Branch Potomac River

Synthetic complementary oligonucleotides are useful hybridization probes for the detection of mRNAs and genes encoding proteins for which only a partial amino acid sequence is known. Usually this involves...Full Text Available

1988-03-01

82

In vivo footprinting of the human [alpha]-globin locus upstream regulatory element by guanine and adenine ligation-mediated polymerase chain reaction

The US Department of the Army, Baltimore District Corps of Engineers, oversees a long-term monitoring study to assess and evaluate effects of the Jennings-Randolph reservoir on biota in the North Branch Potomac River. The reservoir was intended, in part, to mitigate effects of acid mine drainage originating in upstream and headwater area. The present study assessed recovery of benthos and fish in this system, six years after completion of the reservoir. Higher pH and lower iron and sulfate concentrations were observed upstream of the reservoir compared to preimpoundment conditions, suggesting better overall water quality in the upper North Branch. Water quality improved slightly directly downstream of the reservoir. However, the reservoir itself was poorly colonized by macrophytes and benthic organisms, and plankton composition suggested either metal toxicity and/or nutrient limitation. One large tributary to the North Branch and the reservoir ...

83

Effects of acid mine drainage on a headwater stream ecosystem in Colorado

A major regulatory element required for expression of the human [alpha]-globin genes is located 40 kb upstream of the embryonic [zeta]-globin gene. To understand how this and other locus control region (LCR) elements contribute to high-level expression in erythroid cells, we have performed high-resolution, in vivo dimethyl sulfate footprinting. In addition, we have modified the dimethyl sulfate-based ligation-mediated polymerase chain reaction in vivo footprinting procedure to permit the assessment of interactions at guanine and adenine residues, rather than guanines alone. In vivo footprinting of the human [alpha]-LCR element carried on chromosome 16 in a mouse erythroleukemia cell environment revealed protein occupancy at GATA-1, AP-1/NF-E2, and CACC/GGTGG motifs, specific differences compared with in vitro protein binding, and distinct changes in one region upon dimethyl sulfoxide-induced cellular maturation. No protein contacts were detected in nonexpressing ...

1992-05-01

84

Handbook: Approaches for the Remediation of Federal Facility ...

The ecological effects of acid mine drainage were investigated during the summer of 1993 on St. Kevin Gulch, a headwater stream near Leadville, Colorado. The stream currently receives acidic water from an abandoned mine. The pH downstream of the mine is between 3.5 and 4.5, and several metals exceed concentrations toxic to aquatic organisms. Zinc is present at especially high concentrations (1 to 10 mg/L) Furthermore, the stream bottom is covered with a thick layer of iron hydroxide precipitates. Effects on stream biota have been dramatic. Aquatic flora in the affected reach is limited to a green filamentous alga, Ulothrix subtilissima. Macroinvertebrate densities are significantly lower in the affected reach (mean = 99 indiv/m"2; SD = 88 indiv/M"2) compared to an upstream (pristine) reference reach (mean = 1,735 indiv/m"2; SD = 652 indiv/M"2). Functional processes were also studied in the stream. Net primary production (NPP) was measured during midday with ...

85

Genomics - from Neanderthals to high-throughput sequencing

... 4-4 UXO disposal operations ... testing of sequencing batch reactor treatment of ... and lead toward the anode compartment ..... ...

1993-09-01

86

Flip-flop sequence for restoring Zeeman order in the laboratory reference frame

A report on 'The Biology of Genomes' meeting, Cold Spring Harbor, USA, 10-14 May 2006.

2006-01-01

87

Chromosomal localization and cDNA sequence of human BTEB, a GC box binding protein

... spin-lattice relaxation angular momentum electronic circuits magnetic

88

Automated purification of Borrelia burgdorferi s.l. PCR products with KingFisher"T"M magnetic particle processor prior to genome sequencing

Human BTEB cDNA clones have been isolated, sequenced, and the corresponding gene has been assigned to human chromosome 9, region q13, by fluorescent in situ hybridization and DNA blot analysis using DNAs from hybrid cell clones containing a single human chromosome. The cDNA clone encodes a polypeptide of 244 amino acids whose sequence shows a high sequence similarity with the rat BTEB (98% amino acid identity).

1993-09-01

89

Sequence and Organization of pXO1, the Large Bacillus anthracis Plasmid Harboring the Anthrax Toxin Genes

Borrelia burgdorferi sensu lato genospecies were differentiated by PCR-based sequencing of the borrelial flagellin gene. To evaluate the usefulness of KingFisher"T"M magnetic particle processor in PCR product purification, borrelia PCR products were purified with KingFisher"T"M magnetic particle processor prior to cycle sequencing and the quality of the sequence data received was analyzed. KingFisher was found to offer a rapid and reliable alternative for borrelial PCR product purification.

90

On the spectral sequence from Khovanov homology to Heegaard Floer homology

The Bacillus anthracis Sterne plasmid pXO1 was sequenced by random, “shotgun” cloning. A circular sequence of 181,654 bp was generated. One hundred forty-three open...Full Text Available

1999-10-01

91

Nucleotide sequence of a Euglena gracilis chloroplast gene coding for the 16S rRNA: homologies to E. coli and Zea mays chloroplast 16S rRNA.

Ozsvath and Szabo show that there is a spectral sequence whose E^2 term is the reduced Khovanov homology of L, and which converges to the Heegaard Floer homology of the (orientation reversed) branched double cover of S^3 along L. We prove that the E^k term of this spectral sequence is an invariant of the link L when k>2. If L is a transverse link, then we show that Plamenevskaya's transverse invariant gives rise to a transverse invariant of L in each of these higher terms.

2008-01-01

92

Nucleotide sequence and genetic analysis of a 13.1-kilobase-pair Pseudomonas denitrificans DNA fragment containing five cob genes and identification of structural genes encoding Cob(I)alamin adenosyltransferase, cobyric acid synthase, and bifunctional cobinamide kinase-cobinamide phosphate guanylyltransferase.

The nucleotide sequence of 16S rDNA from Euglena gracilis chloroplasts has been determined representing the first complete sequence of an algal chloroplast rRNA gene. The structural part of the 16S...Full Text Available

1982-10-25

93

Moderate deviations for stationary sequences of Hilbert valued bounded random variables

A 13.1-kb DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment contained five different cob genes named...Full Text Available

1991-10-01

94

Isolation, sequence, and expression in Escherichia coli of an unusual thioredoxin gene from the cyanobacterium Anabaena sp. strain PCC 7120.

In this paper, we derive the moderate deviation principle for stationary sequences of bounded random variables with values in a Hilbert space. The conditions obtained are expressed in terms of martingale-type conditions. The main tools are martingale approximations and a new Hoeffding inequality for non adpated sequences of Hilbert-valued random variables. Applications to Cramer-Von Mises statistics, functions of linear processes and stable Markov chains are given.

2008-01-01

95

Isolation and sequence determination of 5'-terminal oligonucleotide fragments of RNA transcripts synthesized by bacteriophage T3-induced RNA polymerase from T3 DNA.

Two sequences with homology to a thioredoxin oligonucleotide probe were detected by Southern blot analysis of Anabaena sp. strain PCC 7120 genomic DNA. One of the sequences was shown to code for a protein...Full Text Available

1989-01-01

96

Influence of rotation and magnetic field on the minimum mass of a main-sequence star

The nucleotide sequence of the 5'-terminal oligonucleotides produced by pancreatic RNase digestion of bacteriophage T3 RNA polymerase (EC 2.7.7.6) transcripts of T3 DNA has been determined. The sequence...Full Text Available

1980-07-01

97

Deep short-read sequencing of chromosome 17 from the mouse strains A/J and CAST/Ei identifies significant germline variation and candidate genes that regulate liver triglyceride levels

The influence of rigid-body and differential rotation and of a fine-scale chaotic magnetic field and a poloidal magnetic field on the minimum mass of a main-sequence star is investigated. It is shown that rotation and a magnetic field with an energy equal to 10--20% of the star's gravitational energy increase the minimum mass of a main-sequence star by 1.5--2 times.

1980-11-01

98

Searching for immunomodulatory sequences in sea bass (Dicentrarchus labrax L.): Transcripts analysis from thymus

Genome sequences are essential tools for comparative and mutational analyses. Here we present the short read sequence of mouse chromosome 17 from the Mus musculus domesticus derived...Full Text Available

2009-01-01

99

British Library Electronic Table of Contents (United Kingdom)

The thymus is a key organ of the immune system in most vertebrates and, for this reason, it has been used in this paper for the generation of a normalized cDNA library from sea bass (Dicentrarchus labrax), one of the most extensively cultured species in South Mediterranean aquaculture. A total of 1632 ESTs from this library were initially analysed for sequence quality and vector sequences and, after this control, 1264 (77% of total clones sequenced) high-quality ESTs were further processed. The total collection of D. labrax thymus ESTs has been deposited in the EBI-GenBank-DBJ database (GenBank accession numbers from FN565576 to FN566839). The functional classification of ESTs was performed by Gene Ontology and KEGG annotation and, successively, the sequences were analysed using the Immuno...

2010-01-01

100

PDBpaint, a visualization webservice to tag protein structures with sequence annotations

British Library Electronic Table of Contents (United Kingdom)

Summary: Protein features are often displayed along the linear sequence of amino acids that make up that protein, but in reality these features occupy a position in the folded proteins 3D space. Mapping sequence features to known or predicted protein structures is useful when trying to deduce the function of those features and when evaluating sequence or structural predictions. To facilitate this goal, we developed PDBpaint, a simple tool that displays protein sequence features gathered from bioinformatics resources on top of protein structures, which are displayed in an interactive window (using the Jmol Java viewer). PDBpaint can be used either with existing protein structures or with novel structures provided by the user. The current version of PDBpaint allows the visualization of annot...

2011-01-01

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101

High-resolution sequence stratigraphic framework and its characteristic. Case study of C-P coal measures in Hebei

High resolution sequence stratigraphy in China

The framework of time-sequence stratigraphy was established according to the point of view of high-resolution sequence stratigraphy. The development characteristic of the sequence, the distribution pattern of strata, the basin evolution and filling, and the relationship between variations of base level and coal accumulation of epicontinental basin were expounded based on the base-level cycles. The result shows that the C-P coal measures in Hebei is a series of basin deposit of inner epicontinental sea. The formation and evolution and sedimentation of the basin is intimately related to the changes of the basin base-level, which controls the development of the coal-accumulation, forming a clear coal-bearing sequence in the cycle. 9 refs., 3 figs.

2001-07-01

102

Heapable Sequences and Subsequences

Since high resolution sequence stratigraphy was introduced into China by DENG Hong-wen in 1995, it has been experienced two development stages in China which are the beginning stage of theory research and development of theory research and application, and the stage of theoretical maturity and widely application that is going into. It is proved by practices that high resolution sequence stratigraphy plays more and more important roles in the exploration and development of oil and gas in Chinese continental oil-bearing basin and the research field spreads to the exploration of coal mine, uranium mine and other strata deposits. However, the theory of high resolution sequence stratigraphy still has some shortages, it should be improved in many aspects. The authors point out that high resolution sequence stratigraphy should be characterized quantitatively and modelized by computer techniques. (authors)

2008-11-01

103

Cloning of the cDNA and gene for a human D sub 2 dopamine receptor

Let us call a sequence of numbers heapable if they can be sequentially inserted to form a binary tree with the heap property, where each insertion subsequent to the first occurs at a leaf of the tree, i.e. below a previously placed number. In this paper we consider a variety of problems related to heapable sequences and subsequences that do not appear to have been studied previously. Our motivation for introducing these concepts is two-fold. First, such problems correspond to natural extensions of the well-known secretary problem for hiring an organization with a hierarchical structure. Second, from a purely combinatorial perspective, our problems are interesting variations on similar longest increasing subsequence problems, a problem paradigm that has led to many deep mathematical connections. We provide several basic results. We obtain an efficient algorithm for determining the heapability of a sequence, and also prove ...

2010-01-01

104

Authentication of byte sequences

A clone encoding a human D{sub 2} dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D{sub 2} receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.

1989-12-01

105

Reservoir anatomy within a sequence stratigraphic framework: The Pennsylvanian Breathitt group of Eastern Kentucky

Algorithms for the authentication of byte sequences are described. The algorithms are designed to authenticate data in the Storage, Retrieval, Analysis, and Display (SRAD) Test Data Archive of the Radiation Effects and Testing Directorate (9100) at Sandia National Laboratories, and may be used in similar situations where authentication of stored data is required. The algorithms use a well-known error detection method called the Cyclic Redundancy Check (CRC). When a byte sequence is authenticated and stored, CRC bytes are generated and attached to the end of the sequence. When the authenticated data is retrieved, the authentication check consists of processing the entire sequence, including the CRC bytes, and checking for a remainder of zero. The error detection properties of the CRC are extensive and result in a reliable authentication of SRAD data.

1991-06-01

106

Precision Measurements of the Cluster Red Sequence using an Error Corrected Gaussian Mixture Model

Detailed quantitative sequence stratigraphic analysis and three-dimensional (3-D) modeling of the fluvio-deltaic Pikeville, Hyden, and four Corners formations of the Breathitt Group, eastern Kentucky, indicate that it is possible to make confident deterministic correlations of sand bodies at offshore development well spacings. The key to high-confidence correlation is the delineation of the 3-D architecture of third-order composite sequences, which are seismically mappable, and the recognition of internal fourth-order sequences, which are stacked into lowstand, transgressive, and highstand sequence sets. Volumetric partitioning of reservoir sand bodies between systems tracts and sequences allows prediction of channel sandstone geometries and channel: crevasse splay ratios. Furthermore, channel-fill type is predictable from the sequence stratigraphy. Within 13 ...

1993-09-01

107

cDNA cloning and sequence analysis of preproendothelin-1 (PPET-1) from salmon, Oncorhynchus keta.

The red sequence is an important feature of galaxy clusters and plays a crucial role in optical cluster detection. Measurement of the slope and scatter of the red sequence are affected both by selection of red sequence galaxies and measurement errors. In this paper, we describe a new error corrected Gaussian Mixture Model for red sequence galaxy identification. Using this technique, we can remove the effects of measurement error and extract unbiased information about the intrinsic properties of the red sequence. We use this method to select red sequence galaxies in each of the 13,823 clusters in the maxBCG catalog, and measure the red sequence ridgeline location and scatter of each. These measurements provide precise constraints on the variation of the average red galaxy populations in the observed frame with redshift. We find that the ...

2009-07-01

108

Performance evaluation for ML sequence detection in ISI channels with Gauss Markov Noise

The presence of endothelin (ET)-like immunoreactivity and the cardiovascular effects of mammalian ET-1 in fish have been reported. To identify ET-related peptides in fish, we screened the cDNA library of the salmon (Oncorhynchus keta) stomach by means of rapid amplification of cDNA ends, and we cloned cDNAs encoding an ET-related peptide. The salmon ET-related sequence of 21 amino acids is identical to the trout ET-1 peptide recently purified from kidney specimens of Oncorhynchus mykiss. The deduced amino acid sequence of salmon pre-proET-1 (PPET-1) comprises 244 amino acids, including a putative signal sequence and mature ET-1, as well as big ET-1 and ET-1-like sequences. This precursor, the first reported PPET-1 sequence for Salmoniformes, Teleostei, has low homology with the sequences of human, mouse, frog (Xenopus laevis), and zebrafish (Danio rerio) PPET-1 ...

2006-06-01

109

High-resolution sequence stratigraphy of a Hettangian-Sinemurian parallic succession, Bornholm, Denmark

Inter-symbol interference (ISI) channels with data dependent Gauss Markov noise have been used to model read channels in magnetic recording and other data storage systems. The Viterbi algorithm can be adapted for performing maximum likelihood sequence detection in such channels. However, the problem of finding an analytical upper bound on the bit error rate of the Viterbi detector in this case has not been fully investigated. Current techniques rely on an exhaustive enumeration of short error events and determine the BER using a union bound. In this work, we consider a subset of the class of ISI channels with data dependent Gauss-Markov noise. We derive an upper bound on the pairwise error probability (PEP) between the transmitted bit sequence and the decoded bit sequence that can be expressed as a product of functions depending on current and previous states in the (incorrect) decoded sequence and the ...

2010-01-01

110

High resolution sequence stratigraphy, sedimentology, petrology and reservoir potentials of the Glauconitic member in Westerose and adjacent fields, Alberta, Canada

Sequence stratigraphic interpretation of paralic successions is complicated by the complex interfingering of marine and continental strata. The successions may also include terrestrial extensions of marine parasequences and completely independent lacustrine parasequence analogues. Failure in recognizing the possible interbedding of these two independent parasequence types may lead to construction of sequence stratigraphic schemes based on incompatible data sets. We have studied a Lower Jurassic paralic section from the Baltic island of Bornholm. The Hettangian-Sinemurian Sose Bugt Member (Ronne Formation) of Bornholm includes lacustrine, fluvial and restricted marine, estuarine deposits reflecting the basin-margin position. Biostatigraphic resolution is poor and a sequence stratigraphic interpretation of the paralic succession is far from straightforward. A multidisciplinary approach including facies analysis, recognition ...

1995-04-01

111

Understanding consumption-related sucralose emissions - A conceptual approach combining substance-flow analysis with sampling analysis

This thesis studied the petrology and sequence stratigraphy of the Hoadley barrier, a Glauconitic member in the Westerose field in Alberta. The unit contains gas reserves in an extensive northeast-southwest trending marine complex. A high resolution sequence stratigraphic model was used to understand the facies development of the unit and the diagenetic pathway of the strata. Six high frequency sequences were identified within the Glauconitic member. Each are separated from one another by surfaces of incision or subaerial exposure which sometimes coincide with a flooding surface and erosion. This thesis provided a petrographic characterization and depositional history of each of the 6 sequences. Reservoir quality is developed within the relatively quartz-rich foreshore facies of the G2B sandstones. The G3 chert litharenite to sublitharenite sandstones are excellent gas producers because of their coarser ...

2002-07-01

112

British Library Electronic Table of Contents (United Kingdom)

This paper explores the potential of combining substance-flow modelling with water and wastewater sampling to trace consumption-related substances emitted through the urban wastewater. The method is exemplified on sucralose. Sucralose is a chemical sweetener that is 600 times sweeter than sucrose and has been on the European market since 2004. As a food additive, sucralose has recently increased in usage in a number of foods, such as soft drinks, dairy products, candy and several dietary products. In a field campaign, sucralose concentrations were measured in the inflow and outflow of the local wastewater treatment plant in Linkoping, Sweden, as well as upstream and downstream of the receiving stream and in Lake Roxen. This allows the loads emitted from the city to be estimated. A method c...

2010-01-01

113

Thermo-hydraulic characterization of an automotive intercooler for a low pressure EGR application

British Library Electronic Table of Contents (United Kingdom)

In this work an experimental study is carried out to determine the thermo-hydraulic performance of an intercooler (IC) with flat tubes provided with triangular plain internal fins and louvered external fins when it is used on a car equipped with a low pressure EGR. The main unknowns to be answered are the thermo-hydraulic characteristics of the IC working under humid conditions induced by EGR, the conditions under which the water content in the mixture of air and exhaust gases begins to condense and the conditions under which the condensed water will be retained inside the IC. The exhaust gases are here replaced by a mixture of dry air and water vapour which are mixed upstream of the IC. The IC is submitted at the following testing conditions: on the ambient air side, the air temperature i...

2011-01-01

114

Surveillance of terrestrial and aquatic environment of the Chinon nuclear power plant. Year 2002-2003; Surveillance de l'environnement terrestre et aquatique de la centrale de Chinon. Annee 2002-2003

Strategy for forecasting ice breakup and jamming: Saint John River, New Brunswick

We find dosage of gamma emitters radionuclides of samples collected in the environment of the Chinon nuclear power plant for 2002 and 2003, these results are presented in two tables: concentrations in gamma emitter radionuclides of samples collected in the terrestrial environment (soils) and aquatic environment (sediments) of the site of Chinon in june 2002 in the frame of the annual surveillance, the second table concerns the concentrations in gamma emitter radionuclides of samples collected in the terrestrial environment (soils and lichens) of the site of Chinon in may 2003 in the frame of the annual surveillance. We find also the dosage of free tritium in the water of the Loire river for 2002 and 2003, upstream and downstream to the power plant of Chinon. (N.C.)

2003-07-01

115

Safety calculation for an underground repository for radioactive waste: the first objective of the alliances calculation software platform

Development of a strategy for forecasting river ice breakup and jamming on the Saint John River in New Brunswick was described. Methods used to identify ice jam-prone areas by simple temperature-based methods to fairly complex computer models using site specific data, were summarized. Recent technological advances in developing forecasts, including the NWRI probe for remotely measuring ice jam thickness, the RIVJAM model for determining stages upstream of an ice jam, and computational procedures for estimating the thrust exerted by ice jams against bridges, were also reviewed. Criteria and recommendations for river ice breakup and ice jamming for the Saint John River were provided. (Abstract only).

1995-12-31

116

Reliability analysis of the downstream face of a rockfill dam. Introductory work

The aim of the safety calculation is to quantify through numerical modelling the radiological impact (molar flow, human dose) of a potential repository for radioactive waste on the Meuse/Haute Marne site at Bure. A selection process is underway for safety scenarios and their phenomenological and numerical conceptual models upstream from the safety calculation. This involves defining and quantifying the geometric and dimensional representations of the repository for each scenario plus the physical, mathematical and numerical models that reflect its behaviour and the uncertainties associated with all the parameters required to quantify the impact. A summary will be given of these various aspects. The numerical simulations are then performed on the Alliances platform which integrates the various computer codes required for the physical representation of the system. (authors)

2005-03-14

117

Radiation Protection Aspects of the Linac Coherent Light Source Front End

Reliability analysis is now being used to a large extent for several civil engineering structures. Such analysis has not been used for rockfill dams, the most common dams in Norway. Following work on reliability analysis for breakwaters, reliability analysis was then used to investigate the ability of the stone material on the upstream face of a rockfill dam to withstand wind generated waves. An attempt is made to follow up this work by including discharge overtopping on a rockfill dam in a reliability analysis. This report primarily goes through previous work on the required weight of protective stones on the downstream face of the dam for specified discharges over the dam and also deals with the probability formulation of extreme floods. A failure function with partial coefficients is formulated. But for reasons of economy the calibration of the partial coefficients remains for a next phase of the project. 14 refs., 17 figs.

1995-02-01

118

Melatonin exerts differential actions on X-ray radiation-induced apoptosis in normal mice splenocytes and Jurkat leukemia cells

The Front End Enclosure (FEE) of the Linac Coherent Light Source (LCLS) is a shielding housing located between the electron dump area and the first experimental hutch. The upstream part of the FEE hosts the commissioning diagnostics for the FEL beam. In the downstream part of the FEE, two sets of grazing incidence mirror and several collimators are used to direct the beam to one of the experimental stations and reduce the bremsstrahlung background and the hard component of the spontaneous radiation spectrum. This paper addresses the beam loss assumptions and radiation sources entering the FEE used for the design of the FEE shielding using the Monte-Carlo code FLUKA. The beam containment system prevents abnormal levels of radiations inside the FEE and ensures that the beam remains in its intended path is also described.

2010-08-26

119

British Library Electronic Table of Contents (United Kingdom)

Abstract: The ability of melatonin as a potent antioxidant was used as a rationale for testing its antiapoptotic ability in normal cells. Recently, melatonin was shown to possess proapoptotic action by increasing reactive oxygen species in certain cancer cells. The modification of radiation-induced apoptosis by melatonin and the expression of apoptosis-associated upstream regulators were studied in normal mice splenocytes and Jurkat T leukemia cells. C57BL/6 mice were exposed to a single whole body X-ray radiation dose of 2 Gy with or without 250 mg/kg melatonin pretreatment. The Jurkat cells were divided into four groups of control, 1 mm melatonin alone, 4 Gy irradiation-only and melatonin pretreatment before irradiation. The highest level of apoptosis in the normal splenic white pulp was...

2009-01-01

120

Heat-transfer augmentation in rod bundles near grid spacers

Heat transfer augmentation of mixed convection through vortex shedding from an inclined plate in a vertical channel containing heated blocks

Heat-transfer augmentation by straight grid spacers in rod bundles is studied for single-phase flow and for post-critical heat flux dispersed flow. The heat transfer effect of swirling grid spacers in single-phase flow is also examined. Governing heat-transfer mechanisms are analyzed, and predictive formulations are established. For single-phase flow, the local heat transfer at a straight spacer and at its upstream or downstream locations are treated separately. The effect of local velocity increasing near swirling spacer is considered. For post critical heat flux (CHF) dispersed flow, the heat transfer by thermal radiation, fin cooling, and vapor convection near the spacer are calculated. The predictions are compared with experimental data with satisfactory agreement.

1982-01-01

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121

Flow field and heat transfer associated with laminar flow over a forward-facing step

A numerical analysis is made of the unsteady flow and heat transfer characteristics of mixed convection in a vertical block-heated channel with and without installing an inclined plate above an upstream block. Parameter studies including the inclined plate angle, Reynolds number (ranging from 260 to 530), and Grashof number (in the range of 0--3,200,000) on heat transfer performance have been explored in detail. The results show that the installation of an inclined plate in the vertical block-heated channel can effectively augment the blocks` heat transfer performance in the channel. This can be applied to heat exchangers.

1998-02-06

122

Energy saving self-powered industrial dehumidifier

The flow and heat transfers associated with a plane laminar flow past a forward-facing step were analyzed using a power-law numerical scheme combined with a false vorticity-stream function approach. To improve the traditional wall-vorticity boundary condition, a novel method, based on an accurate description of the nonslip wall condition, was developed and utilized. The convergence for a 56 x 49 grid system was obtained in about 350 iterations. The computed reattachment distances in the upper separated region agree with the available experimental data for a blunt plate. The heat transfer augmentation is significant across the step; however, it is counterbalanced by the deterioration of heat transfer immediately upstream of the step.

1986-01-01

123

Development towards optimization of emergency countermeasures

An energy saving self-powered industrial dehumidifier for use in a building having a tap water conduit leading from a source of supply to utilization points is described comprising: a dehumidifying apparatus positioned in a stream of air to be dehumidified in the building. The dehumidifying apparatus comprising conduit means for diverting at least a portion of a relatively continuously moving stream of tap water from the tap water conduit through a heat exchanger positioned in a stream of air and returning the diverted portion to the tap water stream upstream of the utilization points. Water vapor in the stream of air will condense onto the heat exchanger when the tap water has a temperature below a dew point temperature of the air stream, thereby dehumidifying the air stream.

1988-07-26

124

Cooling device for impurity removal device in thermonuclear devices

We report on severe accident scenarios consequences evaluation in connection to the applied emergency countermeasures and use of the PC COSYMA code. We present some of the results for the reactor core melt accident assumed to happen at the 632 MWE PWR Krsko Nuclear Power Plant in Slovenia. The efficiency of several potential countermeasures in limiting the late health effects was studied. Regarding the source term, the majority of release parameters are as specified for category 2 in the German Risk Study. Site specific data were used. As the outside (meteorologic) conditions during the potential accident onset can be very different, the study limited to the deterministic runs, assuming the wind direction upstream the Sava river into the WNW direction, wind speed of 5 ms -1 and the C Pasquill stability category. The population distribution file was formed from the NEK-FSAR data for the 1991. (author)

1995-09-11

125

Assessment of the sensitivity and prediction uncertainty of evaporation models applied to Nasser Lake, Egypt

Purpose: To prevent structure material meltdown upon rupture of cooling pipeways in a impurity remover by preventing the coolants from flowing into the vacuum vessel while continuing the supply of coolants to other portions to be cooled. Constitution: Dual cooling pipeway systems are disposed to the neutralizing plates of the impurity remover. A rupture detector (pressure gage) is mounted to each of the cooling pipeways and flow rate control valves to be opened and closed by the signal from the detector are disposed to the upstream and downstream of the cooling pipeway. In this constitution if the cooling pipes should be ruptured, the coolant supply is stopped to the ruptured system in which the flow rate valve is closed by the signal from the rupture detector. However, since the coolant is kept to be supplied to the other system of the cooling pipeways, meltdown of the neutralizing plates can be prevented. (Kamimura, M.).

1983-08-26

126

British Library Electronic Table of Contents (United Kingdom)

SummaryThis paper examines the sensitivity and uncertainty of evaporation estimates from Nasser Lake, south of Egypt using data collected at the floating station Raft, 2km upstream of the Aswan High Dam, and 12 evaporation models. Based on their approach the evaporation models were ranked in five classes, respectively the Bowen ratio energy budget (BREB) approach, three energy-aerodynamic based methods, a mass transfer method, two temperature and five solar radiation-temperature approaches. State dependent parameter models were used to estimate the sensitivity indices of the different evaporation estimation methods at daily and monthly frequencies. A drastic reduction in the cost of the sensitivity analysis was obtained when coupled with efficient sampling methods, producing quasi-Monte Ca...

2010-01-01

127

Analysis of postulated FFTF pipe ruptures

An innovative method of providing total breaker failure protection

A detailed assessment of the FFTF Primary Heat Transport System (PHTS) piping has led to the conclusion that the integrity of the piping is assured such that there is no realistic potential for a rupture. Nevertheless, consistent with the practice of showing design margins even for hypothetical events, a spectrum of postulated PHTS ruptures has been analyzed. The analyses showed that upstream of the reactor vessel inlet downcomer, rupture areas of any size including a double-ended rupture could be tolerated with no core coolant boiling. At the most limiting location, the reactor inlet nozzle, rupture areas of 75 in."2 and 55 in."2 could be tolerated for three-loop and two-loop operation, respectively. This paper will present the following: (1) the criterion with which consequences of postulated pipe ruptures are compared; (2) the general transient response of the FFTF to postulated ruptures; and (3) the acceptable rupture sizes for the FFTF primary loop for a ...

128

The identification of the 1/2"+[660] proton orbitals at high spins in rare-earth nuclei

Breaker failure relaying has been generally achieved through the use of a current monitoring relay to determine whether current continues to flow into a fault after a breaker has been instructed to interrupt the circuit. If current continues to flow after a predefined period of time, the circuit breaker is considered to have failed. Steps must then be taken to trip the next set of upstream breakers in the power system to remove the faulted circuit and prevent system damage. However, with industrial power systems, this may be the utility`s breakers on the feeding transmission line. Regardless, breaker failure schemes must be designed to isolate both the faulted circuit and the failed circuit breaker. This paper discusses a new and innovative method of protecting a circuit breaker from the failure described above, plus other failures that go unprotected with conventional schemes, this providing total breaker failure protection.

1996-09-01

129

Study on high-resolution sequence stratigraphy framework of uranium-hosting rock series in Qianjiadian sag

Decay sequences based on the 1/2"+[660] proton orbital have been identified in "1"7"1Ta and "1"7"7Re based on spin, parity, and large alignment. This decay sequence is observed higher in energy than predicted in cranking calculations based on modified oscillator potentials. Similarly known 1/2"-[541] decay sequences in these and other neighbouring isotopes are observed lower in energy than predicted. A reduction in the strength of the spin-orbit potential for protons is suggested as a solution to these problem. (orig.).

130

Short term relative sea-level oscillations in Upper Devonian Nisku Formation (Alberta, Canada): application to hydrocarbon exploration and recent discoveries

The ore-hosting Yaojia Formation is composed of a set of braided stream medium-fine grained sediments. Guided by the basic theory of high-resolution sequence stratigraphy, and based on the core observation, the analysis of chemical composition of rocks, and data of natural potential logging and apparent resistivity logging, the authors have set up the high-resolution sequence stratigraphy framework of the ore-hosting Yaojia Formation, and discussed the relation of the stratigraphic structure of the middle cycle, as well as the paleotopography, the micro-facies to the formation of uranium deposit. (authors)

2005-07-01

131

Probabilistic risk assessment course documentation. Volume 5. System reliability and analysis techniques Session D - quantification

The relationship between short term relative sea-level oscillations and the reef, off-reef deposits geometry of an Upper Devonian third order sequence highstand of the Nisku Formation in west central Alberta was studied through the analysis of high resolution sequence stratigraphy. Hydrocarbon generation was reconstructed by total organic carbon values and migration patterns that were dependent on the geometry of three fourth order sequences. This stratigraphic reconstruction provided the key to recent hydrocarbon discoveries such as in the Brazeau southern reef margin.

1997-09-01

132

Multi-frequency binary sequence testing at FFTF [Fast Flux Test Facility

This course in System Reliability and Analysis Techniques focuses on the probabilistic quantification of accident sequences and the link between accident sequences and consequences. Other sessions in this series focus on the quantification of system reliability and the development of event trees and fault trees. This course takes the viewpoint that event tree sequences or combinations of system failures and success are available and that Boolean equations for system fault trees have been developed and are available. 93 figs., 11 tabs.

1985-08-01

133

Identification of the 1/2/sup +/(660) proton orbitals at high spins in rare-earth nuclei

The multi-frequency binary sequence experimental technique has been implemented at the Fast Flux Test Facility for routine surveillance activities. The frequency content of the standard rod-movement sequence has been shown to be sufficient to normalize the data at moderate frequencies. This obviates the need for auxiliary calibration measurements and provides the reactivity worth of the test control rod. Analyses of a series of tests conducted in 1986 illustrate that the rod worths inferred from the tests are consistent with zero-power measurements. Also, the dependence of the prompt feedback time constant on reactor conditions was determined.

1988-09-18

134

Engineering Relative Compression of Genomes

Decay sequences based on the 1/2/sup +/(660) proton orbital have been identified in /sup 171/Ta and /sup 177/Re based on spin, parity, and large alignment. This decay sequence is observed higher in energy than predicted in cranking calculations based on modified oscillator potentials. Similarly known 1/2/sup -/(541) decay sequences in these and other neighbouring isotopes are observed lower in energy than predicted. A reduction in the strength of the spin-orbit potential for protons is suggested as a solution to these problem.

1983-12-08

135

Congestion cost allocation method in a pool model

Technology progress in DNA sequencing boosts the genomic database growth at faster and faster rate. Compression, accompanied with random access capabilities, is the key to maintain those huge amounts of data. In this paper we present an LZ77-style compression scheme for relative compression of multiple genomes of the same species. While the solution bears similarity to known algorithms, it offers significantly higher compression ratios at compression speed over a order of magnitude greater. One of the new successful ideas is augmenting the reference sequence with phrases from the other sequences, making more LZ-matches available.

2011-01-01

136

Ultraconserved Elements: Analyses of Dosage Sensitivity, Motifs and Boundaries

The congestion cost caused by transmission capacities and voltage limit is an important issue in a competitive electricity market. To allocate the congestion cost equitably, the active constraints in a constrained dispatch and the sequence of these constraints should be considered. A multi-stage method is proposed which reflects the effects of both the active constraints and the sequence. In a multi-stage method, the types of congestion are analysed in order to consider the sequence, and the relationship between congestion and the active constraints is derived in a mathematical way. The case study shows that the proposed method can give more accurate and equitable signals to customers. (Author)

2003-09-01

137

The COG database: an updated version includes eukaryotes

Ultraconserved elements (UCEs) are sequences that are identical between reference genomes of distantly related species. As they are under negative selection and enriched near or in specific classes...Full Text Available

2008-12-01

138

Systems medicine: the future of medical genomics and healthcare

BackgroundThe availability of multiple, essentially complete genome sequences of prokaryotes and eukaryotes spurred both the demand and the opportunity for the construction of an...Full Text Available

139

Space power systems prelaunch integration

High-throughput technologies for DNA sequencing and for analyses of transcriptomes, proteomes and metabolomes have provided the foundations for deciphering the structure, variation and function of the...Full Text Available

140

Sirtuins, Bioageing, and Cancer

The sequence of events from the assembly of a space nuclear power system to its integration in the Space Shuttle Transportation System (STS) is considered. First, the sequence followed for SNAP-10A, the only free world space reactor electric power system ever launched and operated in space, is reviewed. Before shipment, the SNAP-10A reactor was raised to operating temperature using electrically supplied heat and operated at low power for control calibration. Next we discuss shipment to the launch site, a phase that is critical because of the potential for various accidents. Once the power system arrives at the launch site, the processing sequence is performed. This sequence includes checkout, mating with the payload or upper stage launch vehicle, and integration into the STS.

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141

Sequences of versatile, broad-host-range vectors of the RK2 family.

The Sirtuins are a family of orthologues of yeast Sir2 found in a wide range of organisms from bacteria to man. They display a high degree of conservation between species, in both sequence and function,...Full Text Available

142

Sequence features involved in the mechanism of 3' splice junction wobbling

Plasmid pRK404-a smaller derivative of RK2-is a tetracycline-resistant broad-host-range vector that carries a multiple cloning site and the lacZ(alpha) peptide that enables blue/white selection for cloned inserts in Escherichia coli. We present herein the complete and annotated sequence of pRK404 and three related vectors-pRK437, pRK442, and pRK442(H). These derivatives have proven to be valuable tools for genetic manipulation in Gram-negative bacteria. The knowledge of their complete sequences will facilitate efficient future engineering of them and will enhance their general applicability to the design of genetic systems for use in organisms for which new genomic sequence data are becoming available.

2003-07-01

143

SIM: Stellar Astrophysics - SIM - NASA

BackgroundAlternative splicing is an important mechanism mediating the diversified functions of genes in multicellular organisms, and such event occurs in around 40-60% of human...Full Text Available

144

Pitch discrimination by ferrets for simple and complex sounds

... and one near-main-sequence star, which will stringently constrain calculations of single-star evolution at high metallicity. Independent of SIM Lite observations ...

145

Phylogeny of Steinernema Travassos, 1927 (Cephalobina: Steinernematidae) Inferred From Ribosomal DNA Sequences and ...

Although many studies have examined the performance of animals in detecting a frequency change in a sequence of tones, few have measured animals' discrimination of the fundamental frequency...Full Text Available

2009-09-01

146

Phylogenetic relationships in Cortinarius, section Calochroi, inferred from nuclear DNA sequences

... 0877:POSTCS]2.0.CO;2 Phylogeny of Steinernema Travassos, 1927 (Cephalobina: Steinernematidae) Inferred From Ribosomal DNA ... C...

147

Ovine reference materials and assays for prion genetic testing

BackgroundSection Calochroi is one of the most species-rich lineages in the genus Cortinarius (Agaricales, Basidiomycota) and is widely distributed...Full Text Available

148

BackgroundGenetic predisposition to scrapie in sheep is associated with several variations in the peptide sequence of the prion protein gene (PRNP). DNA-based tests...Full Text Available

149

Natural gas annual 1996

NASA Research Announcement: GALEX GI Program Cycle 2 - GALEX - NASA

This document provides information on the supply and disposition of natural gas to a wide audience. The 1996 data are presented in a sequence that follows natural gas from it`s production to it`s end use.

1997-09-01

150

NASA Research Announcement: GALEX GI Program Cycle 1 - GALEX - NASA

These include, but are not limited to: stellar winds and outflows, post-main- sequence stellar evolution, binary/multiple star evolution, globular cluster ...

151

Menstrual Cycle: Basic Biology

These include, but are not limited to: stellar winds and outflows, post-main- sequence star evolution, binary star evolution, globular cluster structure and ...

152

Mapping Equivalence for Symbolic Sequences: Theory and Applications

The basic biology of the menstrual cycle is a complex, coordinated sequence of events involving the hypothalamus, anterior pituitary, ovary, and endometrium. The menstrual cycle with all its...Full Text Available

2008-01-01

153

Magnetic resonance imaging of chronic aortic dissection

Processing of symbolic sequences represented by mapping of symbolic data into numerical signals is commonly used in various applications. It is a particularly popular approach in genomic and proteomic sequence analysis. Numerous mappings of symbolic sequences have been proposed for various applications. It is unclear however whether the processing of symbolic data provides an artifact of the numerical mapping or is an inherent property of the symbolic data. This issue has been long ignored in the engineering and scientific literature. It is possible that many of the results obtained in symbolic signal processing could be a byproduct of the mapping and might not shed any light on the underlying properties embedded in the data. Moreover, in many applications, conflicting conclusions may arise due to the choice of the mapping used for numerical representation of symbolic data. In this paper, we present a novel framework for ...

2009-01-01

154

MR imaging of the internal auditory canal and inner ear at 3T: comparison between 3D driven equilibrium and 3D balanced fast field echo sequences.

17 patients with chronic aortic dissection were examined by MRI. In 12 patients, comparison between gradient echo sequences and SE sequences was possible. Gradient echo sequences, unlike SE sequences, permitted evaluation of flow in the true and false lumen, reliable differentiation between thrombus and flowing blood and clear delination of the intimal flap. An additional comparison between transoesophageal ultrasound and MRT in 15 patients showed significant advantages in favour of MRI. In three patients MRI was able to detect more proximal origins of the dissection. Moreover, MRI allowed evaluation of the major aortic branches and their relation to the dissection; this was not possible with ultrasound. MRI plays an important role in the follow-up of chronic aortic dissections. (orig.).

155

Isolation and characterization of an ornithine aminotransferase-related sequence (OATL3) mapping to 10q26

Full Text Available

2008-06-01

156

Heavy chain of Acanthamoeba myosine IB is a fusion of myosin-like and non-myosin-like sequences

The authors used a near full-length human ornithine [delta]-aminotransferase cDNA, huOAT6, as a probe under low stringency hybridization conditions to identify a new autosomal ornithine [delta]-aminotransferase-related sequence (OATL3). Cloning and characterization of this sequence reveal it to be a partial nonprocessed pseudogene corresponding to exon 3 and flanking intronic sequences of the ornithine [delta]-aminotransferase structural gene. Using somatic cell hybrids and fluorescence in situ hybridization, they mapped OATL3 to 10q26, adjacent to the ornithine [delta]-aminotransferase structural gene locus. 13 refs., 3 figs.

1993-08-01

157

Heavy chain of Acanthamoeba myosine IB is a fusion of myosin-like and non-myosin-like sequences

Acanthamoeba castellanii myosins IA and IB demonstrate the catalytic properties of a myosin and can support analogues of contractile and motile activity in vitro, but their single, low molecular weight heavy chains, roughly globular shapes, and inabilities to self-assemble into filaments make them structurally atypical myosins. The authors present the complete amino acid sequence of the 128-kDa myosin IB heavy chain, which they deduced from the nucleotide sequence of the gene and which reveals that the polypeptide is a fusion of myosin-like and non-myosin-like sequences. Specifically, the amino-terminal approx. 76 kDa of amino acid sequence is highly similar to the globular head sequences of conventional myosins. By contrast, the remaining approx. 51 kDa of sequence shows no similarity to any portion of conventional myosin sequences, ...

1987-10-01

158

GALAXY EVOLUTION EXPLORER (GALEX) - HEASARC - NASA

Acanthamoeba castellanii myosins IA and IB demonstrate the catalytic properties of a myosin and can support analogues of contractile and motile activity in vitro, but their single, low molecular weight heavy chains, roughly globular shapes, and inabilities to self-assemble into filaments make them structurally atypical myosins. The authors present the complete amino acid sequence of the 128-kDa myosin IB heavy chain, which they deduced from the nucleotide sequence of the gene and which reveals that the polypeptide is a fusion of myosin-like and non-myosin-like sequences. Specifically, the amino-terminal #approx# 76 kDa of amino acid sequence is highly similar to the globular head sequences of conventional myosins. By contrast, the remaining #approx# 51 kDa of sequence shows no similarity to any portion of conventional myosin sequences, ...

159

GALAXY EVOLUTION EXPLORER (GALEX) - GALEX - NASA

These include, but are not limited to: stellar winds and outflows, post-main- sequence stellar evolution, binary/multiple star evolution, globular cluster structure ...

160

Fast evolving 18S rRNA sequences from Solenogastres (Mollusca) resist standard PCR amplification and give new insights into mollusk substitution rate heterogeneity

These include, but are not limited to: stellar winds and outflows, post-main- sequence stellar evolution, binary/multiple star evolution, globular cluster structure ...

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161

Norwegian Open Research Archives (NORA)

Full Text Available

162

Detection of polychlorinated biphenyl degradation genes in polluted sediments by direct DNA extraction and polymerase chain reaction

Detection of genetic mutations in repetitive DNA sequences in somatic human cells after low-dose irradiation

It was the aim of this study to specifically detect the DNA sequences for the bphC gene, the meta-cleavage enzyme of the aerobic catabolic pathway for biphenyl and polychlorinated biphenyl degradation, in aquatic sediments without prior cultivation of microorganisms by using extraction of total DNA, PCR amplification of bphC sequences, and detection with specific gene probes. The direct DNA extraction protocol used was modified to enhance lysis efficiency. Crude extracts of DNA were further purified by gel filtration, which yielded DNA that could be used for the PCR. PCR primers were designed for conserved regions of the bphC gene from a sequence alignment of five known sequences. The specificity of PCR amplification was verified by using digoxigenin-labeled DNA probes which were located internal to the amplified gene sequence. The detection limit for the bphC gene of Pseudomonas ...

1993-12-01

163

Detecting retroviral sequences in chronic fatigue syndrome.

... 3-89701-147-6 0949-2224 245 p. animal cells dna genetic radiation effects

1998-03-01

164

Consensus sequence L/PKSSLL mimics crucial epitope on Loop III of Taiwan cobra cardiotoxin

XMRV or xenotropic murine leukemia virus-related retrovirus, a recently discovered retrovirus, has been linked to both prostate cancer and chronic fatigue syndrome (CFS). Recently, the teams of Drs. Shyh-Ching Lo and Harvey Alter discovered the presence of sequences closely related to XMRV in the blood of 86.5% of patients with CFS [1]. These findings are important because since the initial discovery of XMRV in CFS, several studies have failed to find XMRV in specimens collected from CFS patients. While the current study also did not find XMRV in CFS, Lo et al. did detect sequences that belong to polytropic mouse endogenous retroviruses (PMV), which share considerable similarity with XMRV. Criteria for future studies that will help bring greater clarity to the issue of retroviral sequences in CFS are proposed below. PMID:21994623

2010-11-03

165

British Library Electronic Table of Contents (United Kingdom)

Phage display is effective in screening peptides that mimic venom's neutralizing epitopes. A phage display cyclized heptapeptide library (C7C library) was panned with purified divalent antivenin IgG, which neutralizes Naja naja atra venom (NAV) and Bungarus multicinctus venom (BMV). The selected heptapeptide sequences were aligned with known protein sequences of NAV and BMV in GenBank. One of the four consensus sequences, L/PKSSLL, mimicked the crucial epitope on Loop III of Taiwan cobra cardiotoxin that is associated with the venom's lethal potency. In dot blot analysis, several clones showed varying reactivities for NAV monovalent antivenin and lesser cross-reactions with BMV monovalent antivenin. The KSSLLRN-carrying phage occurred four times in selected clones and showed the strongest ...

2009-01-01

166

Comparative and phylogenomic studies on the mitochondrial genomes of Pentatomomorpha (Insecta: Hemiptera: Heteroptera)

Cis-regulatory mutations in human disease

BackgroundNucleotide sequences and the gene arrangements of mitochondrial genomes are effective tools for resolving phylogenetic problems. Hemipteroid insects are known to possess...Full Text Available

167

Choosing and Using a Plant DNA Barcode

Cis-acting regulatory sequences are required for the proper temporal and spatial control of gene expression. Variation in gene expression is highly heritable and a significant determinant...Full Text Available

2009-07-01

168

Cancer gene discovery in mouse and man

The main aim of DNA barcoding is to establish a shared community resource of DNA sequences that can be used for organismal identification and taxonomic clarification. This approach was successfully...Full Text Available

169

Analysis of the transcriptome of the root lesion nematode Pratylenchus coffeae generated by 454 sequencing technology

AbstractThe elucidation of the human and mouse genome sequence and developments in high-throughput genome analysis, and in computational tools, have made it possible to profile entire...Full Text Available

2009-12-01

170

British Library Electronic Table of Contents (United Kingdom)

To study interactions between plants and plant-parasitic nematodes, several omics studies have nowadays become extremely useful. Since most data available so far is derived from sedentary nematodes, we decided to improve the knowledge on migratory nematodes by studying the transcriptome of the nematode Pratylenchus coffeae through generating expressed sequence tags (ESTs) on a 454 sequencing platform. In this manuscript we present the generation, assembly and annotation of over 325,000 reads from P. coffeae. After assembling these reads, 56,325 contigs and singletons with an average length of 353bp were selected for further analyses. Homology searches revealed that 25% of these sequences had significant matches to the Swiss-prot/trEMBL database and 29% had significant matches in nematode E...

2011-01-01

171

An Application of Supertree Methods to Mammalian Mitogenomic Sequences

A comparison of the X-ray properties of X Per and gamma Cas

Two different approaches can be used in phylogenomics: combined or separate analysis. In the first approach, different datasets are combined in a concatenated supermatrix. In the second, datasets are...Full Text Available

172

A PRELIMINARY PHYLOGENETIC ANALYSIS OF METAPENAEOPSIS (DECAPODA: PENAEIDAE) BASED ON MITOCHONDRIAL DNA SEQUENCES OF ...

The X-ray properties of the main sequence Be stars conclude that they are a widely separated binary system containing an accreting neutron star.

1982-04-01

173

A $G_{4}$ Sequence within PHR1 Promoter Acts as a Gate for Cross-Talks between Damage-Signaling Pathway and Multi-Stress Response

... each species studied are listed in Table 1. Penaeus monodon Fabricius, 1798 (family Penaeidae) and Solenocera koelbeli De Man, ... ...

174

Experimental study of nanoparticles penetration through commercial filter media

Full Text Available

2002-09-01

175

Archive monitoring of pollutants in the Dee River using SIMS analysis of mussels

In this study, nanoparticle penetration was measured with a wide range of filter media using silver nanoparticles from 3 nm to 20 nm at three different face velocities in order to define nanoparticle filtration characteristics of commercial fibrous filter media. The silver particles were generated by heating a pure silver powder source via an electric furnace with a temperature of 870 deg. C, which was found to be the optimal temperature for generating an adequate amount of silver nanoparticles for the size range specified above. After size classification using a nano-DMA, the particle counts were measured by an Ultrafine Condensation Particle Counter (UCPC) both upstream and downstream of the test filter to determine the nanoparticle penetration for each specific particle size. Particle sampling time continued long enough to detect more than 10{sup 5} counts at the upstream and 10 counts at the downstream sampling point so that 99.99% ...

2007-01-15

176

The holographic principle and the language of genes

Full text: Mt Morgan was mined for copper and gold from 1882 to 1982 and subsequent tailings re-processing ceased in 1990. Direct and accidental deposition of tailings into the adjacent Dee River and acid mine drainage from the mine site continues to impact the Dee River. The Queensland Department of Natural Resources gauging and monitoring station at Kenbula (adjacent to the mine) continuously monitors pH of around 2.8. Twenty kilometres downstream of the mine the pH improves to 3.5. Elevated sediment metal concentrations have been recorded over 50 kilometres downstream in investigations conducted by CSIRO (Jones et al. 1995, 1996) and others. Biological monitoring by Mackey (1988) and subsequently by Duivendoorden (1995,1997), confirmed significant impact on the fish, macrophyte of macro invertebrate populations downstream of the mine. The current AINSE sponsored project will investigate the use of mussels (genus Allathryia) to archive the pollutant load in the Dee River using SIMS. ...

2000-11-29

177

The Sorcerer II Global Ocean Sampling Expedition: Expanding theUniverse of Protein Families

We show that the holographic principle in quantum gravity imposes a strong constraint on life. The degrees of freedom of an organism can be estimated according to the theory of Boolean networks, which is constrained by the entropy bound. Hence we can explain the languages in protein sequences or in DNA sequences. The overall evolution of biological complexity can be illustrated. And some general properties of protein length distributions can be explained by a linguistic mechanism.

2008-01-01

178

Targeted Capture and Next-Generation Sequencing Identifies C9orf75, Encoding Taperin, as the Mutated Gene in Nonsyndromic Deafness DFNB79

Metagenomics projects based on shotgun sequencing of populations of micro-organisms yield insight into protein families. We used sequence similarity clustering to explore proteins with a comprehensive dataset consisting of sequences from available databases together with 6.12 million proteins predicted from an assembly of 7.7 million Global Ocean Sampling (GOS) sequences. The GOS dataset covers nearly all known prokaryotic protein families. A total of 3,995 medium- and large-sized clusters consisting of only GOS sequences are identified, out of which 1,700 have no detectable homology to known families. The GOS-only clusters contain a higher than expected proportion of sequences of viral origin, thus reflecting a poor sampling of viral diversity until now. Protein domain distributions in the GOS dataset and current protein databases show distinct biases. Several ...

2006-03-23

179

Sequencing and Comparative Genomic Analysis of pK29, a 269-Kilobase Conjugative Plasmid Encoding CMY-8 and CTX-M-3 ?-Lactamases in Klebsiella pneumoniae?

Targeted genome capture combined with next-generation sequencing was used to analyze 2.9 Mb of the DFNB79 interval on chromosome 9q34.3, which includes 108 candidate genes. Genomic...Full Text Available

2010-03-12

180

SAS1 and SAS2, GTP-binding protein genes in Dictyostelium discoideum with sequence similarities to essential genes in Saccharomyces cerevisiae.

A 269-kilobase conjugative plasmid, pK29, from a Klebsiella pneumoniae strain was sequenced. The plasmid harbors multiple antimicrobial resistance genes, including those encoding CMY-8...Full Text Available

2007-08-01

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181

Primary structure of streptococcal Pep M5 protein: Absence of extensive sequence repeats

We have identified two novel, very closely related genes, SAS1 and SAS2, from Dictyostelium discoideum. These encode small, approximately 20-kilodaton proteins with amino acid sequences thought to be...Full Text Available

1990-05-01

182

Molecular identification of CTX-M and ^blaOXY/K1 ?-lactamase genes in Enterobacteriaceae by sequencing of universal M13-sequence tagged PCR-amplicons

Extensive sequence repeats have been observed in a biologically active fragment of type 24 streptococcal M protein, namely Pep M24 [Beachey, E. H., Sayer, J. M. & Kang, A. H. (1978) Proc....Full Text Available

1983-09-01

183

Molecular cloning of chicken metallothionein. Deduction of the complete amino acid sequence and analysis of expression using cloned cDNA

BackgroundPlasmid encoded ^blaCTX-M enzymes represent an important sub-group of class A β-lactamases causing the ESBL phenotype which is increasingly...Full Text Available

184

Molecular characterization of cap3A, a gene from the operon required for the synthesis of the capsule of Streptococcus pneumoniae type 3: sequencing of mutations responsible for the unencapsulated phenotype and localization of the capsular cluster on the pneumococcal chromosome.

A cDNA library was constructed using RNA isolated from the livers of chickens which had been treated with zinc. This library was screened with a RNA probe complementary to mouse metallothionein-I (MT), and eight chicken MT cDNA clones were obtained. All of the cDNA clones contained nucleotide sequences homologous to regions of the longest (375 bp) cDNA clone. The latter contained an open reading frame of 189 bp, and the deduced amino acid sequence indicates a protein of 63 amino acids of which 20 are cysteine residues. Amino acid composition and partial amino acid sequence analyses of purified chicken MT protein agreed with the amino acid composition and sequence deduced from the cloned cDNA. Amino acid sequence comparison establish that chicken MT shares extensive homology with mammalian MTs. Southern blot analysis of chicken DNA indicates that the chicken MT gene is not a part of ...

1988-01-25

185

High-resolution sequence stratigraphy from outcrop study, with the integration of log and seismic data

The complete nucleotide sequence of the cap3A gene of Streptococcus pneumoniae, which is directly responsible for the transformation of some unencapsulated, serotype 3 mutants to the encapsulated phenotype,...Full Text Available

1994-10-01

186

Glutamine-linked and Non-consensus Asparagine-linked Oligosaccharides Present in Human Recombinant Antibodies Define Novel Protein Glycosylation Motifs

The detailed sequence stratigraphic analysis of the siliciclastic-dominated Late Cretaceous sediments (Aren Sandstone and Garumnian red beds, south central Pyrenees, Spain) reveals the repeating disposition of critical elements and controlling mechanisms of cycles and sequences. Our approach integrates (a) hierarchy of unconformity-bounded units, (b) physical expression of boundaries traceable from the continent to the basin, (c) featuring facies and depositional systems, (d) well log and seismic expression, and (e) driving basing-filling mechanisms. A comparison to other active basins is suggested in order to prove the validity beyond the regional scale. Four basin-wide transgressive facies cycles were identified and interpreted as third-order units. The transgressive phase of each cycle is represented by mixed shelf deposits, while regressive periods consists of complex delta systems. The cycles are composed within their regressive phase of ...

1993-09-01

187

Genomic sequence for human prointerleukin 1 beta: possible evolution from a reverse transcribed prointerleukin 1 alpha gene.

We report the presence of oligosaccharide structures on a glutamine residue present in the V_L domain sequence of a recombinant human IgG2 molecule. Residue Gln-106, present in the QGT sequence...Full Text Available

2010-05-21

188

Extended amino acid sequences around the active-site lysine residue of class-I fructose 1,6-bisphosphate aldolases from rabbit muscle, sturgeon muscle, trout muscle and ox liver.

We have isolated the human prointerleukin 1 (proIL-1) beta gene from leukocyte and fetal liver libraries. The nucleotide sequence and its gene organization reveals that the proIL-1 beta gene is composed...Full Text Available

1986-10-24

189

Exploiting rice-sorghum synteny for targeted development of EST-SSRs to enrich the sorghum genetic linkage map.

1. Amino acid sequences covering the region between residues 173 and 248 [adopting the numbering system proposed by Lai, Nakai & Chang (1974) Science 183, 1204-1206] were derived for trout (Salmo...Full Text Available

1979-11-01

190

Draft Genome Sequence of Shewanella sp. Strain HN-41, Which Produces Arsenic-Sulfide Nanotubes.

The sequencing and detailed comparative functional analysis of genomes of a number of select botanical models open new doors into comparative genomics among the angiosperms, with potential benefits for improvement of many orphan crops that feed large populations. In this study, a set of simple sequence repeat (SSR) markers was developed by mining the expressed sequence tag (EST) database of sorghum. Among the SSR-containing sequences, only those sharing considerable homology with rice genomic sequences across the lengths of the 12 rice chromosomes were selected. Thus, 600 SSR-containing sorghum EST sequences (50 homologous sequences on each of the 12 rice chromosomes) were selected, with the intention of providing coverage for corresponding homologous regions of the sorghum genome. Primer pairs were designed and polymorphism detection ...

2009-08-08

191

Detection of Atopobium vaginae in postmenopausal women by cultivation-independent methods warrants further investigation.

The dissimilatory metal reducing bacterium Shewanella sp. strain HN-41 was first reported to produce novel photoactive As-S nanotubes via reduction of As(V) and S(2)O(3)(2-) under anaerobic conditions. Here we report the draft genome sequence and annotation of strain HN-41. PMID:21868804

2011-09-01

192

Continuous intensity map optimization (CIMO): A novel approach to leaf sequencing in step and shoot IMRT

We sequenced 16S rRNA genes from the vaginal swab contents of a postmenopausal woman with asymptomatic bacterial vaginosis (BV). Sequences from Atopobium vaginae were the most commonly detected. In a survey of 35 other postmenopausal women, this organism was detected in 44% with BV but not in any subjects deemed healthy. PMID:15071062

2004-04-01

193

Cloning, sequence analysis and over-expression of the gene for the class II fructose 1,6-bisphosphate aldolase of Escherichia coli.

A new leaf-sequencing approach has been developed that is designed to reduce the number of required beam segments for step-and-shoot intensity modulated radiation therapy (IMRT). This approach to leaf sequencing is called continuous-intensity-map-optimization (CIMO). Using a simulated annealing algorithm, CIMO seeks to minimize differences between the optimized and sequenced intensity maps. Two distinguishing features of the CIMO algorithm are (1) CIMO does not require that each optimized intensity map be clustered into discrete levels and (2) CIMO is not rule-based but rather simultaneously optimizes both the aperture shapes and weights. To test the CIMO algorithm, ten IMRT patient cases were selected (four head-and-neck, two pancreas, two prostate, one brain, and one pelvis). For each case, the optimized intensity maps were extracted from the Pinnacle"3 treatment planning system. The CIMO algorithm was applied, and the ...

2006-04-01

194

Recurrent plot analysis of discharge sequences in tracking test of polybutylene polymers

Nucleotide sequence analysis of the Escherichia coli chromosomal DNA inserted in the plasmid pLC33-5 of the Clarke and Carbon library [Clarke & Carbon (1976) Cell 9, 91-99] revealed the existence...Full Text Available

1989-01-15

195

Isolation of fetal DNA from nucleated erythrocytes in maternal blood

Polymers are required to use in radiation environments as insulation materials. However, they often suffer from tracking failure. There is an increasing demand to evaluate radiation effects on dielectric performance. This paper presents a recurrence plot (RP) approach to analyse surface discharge sequences of gamma-ray irradiated polymer materials based on tracking test. Studying the non-linear characteristics of discharge sequences can assist in understanding the underlying mechanism of the discharge process. Discharge sequences of the test are extended to m-dimensional phase space by using the phase space reconstructed method. As test samples, polybutylene terephthalate (PBT) and polybutylene naphthalate (PBN) were irradiated to 100 kGy and then up to 1 MGy with a dosage rate of 10 kGy h{sup -1} by using a {sup 60}Co gamma source. The tracking tests were carried out according to the test method described in IEC60112. It ...

2008-10-07

196

Homology analyses of the protein sequences of fatty acid synthases from chicken liver, rat mammary gland, and yeast

Fetal nucleated cells within maternal blood represent a potential source of fetal genes obtainable by venipuncture. The authors used monoclonal antibody against the transferrin receptor (TIR) to identify nucleated erythrocytes in the peripheral blood of pregnant women. Candidate fetal cells from 19 pregnancies were isolated by flow sorting at 12 1/2-17 weeks gestation. The DNA in these cells was amplified for a 222-base-pair (bp) sequence present on the short arm of the Y chromosome as proof that the cells were derived from the fetus. The amplified DNA was compared with standardized DNA concentrations. In the case of the female fetus, DNA prepared from samples at 32 weeks of gestation and cord blood at delivery also showed the presence of the Y chromosomal sequence, suggesting Y sequence mosaicism or translocation. In 10/12 cases where the 222-bp band was absent, the fetuses were female. Thus, they were successful in ...

1990-05-01

197

Bisulfite genomic sequencing of DNA from dried blood spot microvolume samples.

Homology analyses of the protein sequences of chicken liver and rat mammary gland fatty acid synthases were carried out. The amino acid sequences of the chicken and rat enzymes are 67% identical. If conservative substitutions are allowed, 78% of the amino acids are matched. A region of low homologies exists between the functional domains, in particular around amino acid residues 1059-1264 of the chicken enzyme. Homologies between the active sites of chicken and rat and of chicken and yeast enzymes have been analyzed by an alignment method. A high degree of homology exists between the active sites of the chicken and rat enzymes. However, the chicken and yeast enzymes show a lower degree of homology. The DADPH-binding dinucleotide folds of the {beta}-ketoacyl reductase and the enoyl reductase sites were identified by comparison with a known consensus sequence for the DADP- and FAD-binding dinucleotide folds. The active sites ...

1989-11-01

198

Affinity purification of sequence-specific DNA binding proteins.

DNA methylation is an important event in epigenetic changes in cells, and a fundamental regulator of gene transcription. Bisulfite genomic sequencing is a powerful technique used in studies of DNA methylation. However, the established procedures often require relatively large amounts of DNA. In everyday practice, samples submitted for analysis might contain very small amounts of poor quality material, as is often the case with forensic stain samples. In this study, we assess a modified, more efficient method of bisulfite genomic sequencing. Genomic DNA extracted from 3-mm dried blood spots using QIAamp micro kit was treated with sodium bisulfite (using EpiTect kit). Subsequent methylation-specific PCR (MSP) followed by DNA sequencing displayed the differentially methylated region of imprinted gene SNRPN. Our results show that this new combination of efficient DNA extraction and bisulfite treatment provides high quality ...

2011-07-01

199

A Systematic Framework for the Construction of Optimal Complete Complementary Codes

We describe a method for affinity purification of sequence-specific DNA binding proteins that is fast and effective. Complementary chemically synthesized oligodeoxynucleotides that contain a recognition site for a sequence-specific DNA binding protein are annealed and ligated to give oligomers. This DNA is then covalently coupled to Sepharose CL-2B with cyanogen bromide to yield the affinity resin. A partially purified protein fraction is combined with competitor DNA and subsequently passed through the DNA-Sepharose resin. The desired sequence-specific DNA binding protein is purified because it preferentially binds to the recognition sites in the affinity resin rather than to the nonspecific competitor DNA in solution. For example, a protein fraction that is enriched for transcription factor Sp1 can be further purified 500- to 1000-fold by two sequential affinity chromatography steps to give Sp1 of an estimated 90% ...

1986-08-01

200

Mapping a nucleolar targeting sequence of an RNA binding nucleolar protein, Nop25

The complete complementary code (CCC) is a sequence family with ideal correlation sums which was proposed by Suehiro and Hatori. Numerous literatures show its applications to direct-spread code-division multiple access (DS-CDMA) systems for inter-channel interference (ICI)-free communication with improved spectral efficiency. In this paper, we propose a systematic framework for the construction of CCCs based on $N$-shift cross-orthogonal sequence families ($N$-CO-SFs). We show theoretical bounds on the size of $N$-CO-SFs and CCCs, and give a set of four algorithms for their generation and extension. The algorithms are optimal in the sense that the size of resulted sequence families achieves theoretical bounds and, with the algorithms, we can construct an optimal CCC consisting of sequences whose lengths are not only almost arbitrary but even variable between sequence families. We ...

2010-01-01

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201

Depositional and dissolutional processes and their resulting thinning patterns within the Middle Devonian Prairie Formation, Williston basin, North Dakota and Montana

Nop25 is a putative RNA binding nucleolar protein associated with rRNA transcription. The present study was undertaken to determine the mechanism of Nop25 localization in the nucleolus. Deletion experiments of Nop25 amino acid sequence showed Nop25 to contain a nuclear targeting sequence in the N-terminal and a nucleolar targeting sequence in the C-terminal. By expressing derivative peptides from the C-terminal as GFP-fusion proteins in the cells, a lysine and arginine residue-enriched peptide (KRKHPRRAQDSTKKPPSATRTSKTQRRRR) allowed a GFP-fusion protein to be transported and fully retained in the nucleolus. When the peptide was fused with cMyc epitope and expressed in the cells, a cMyc epitope was then detected in the nucleolus. Nop25 did not localize in the nucleolus by deletion of the peptide from Nop25. Furthermore, deletion of a subdomain (KRKHPRRAQ) in the peptide or amino acid substitution of lysine and arginine ...

2006-06-10

202

DNA rearrangements from #gamma#-irradiated normal human fibroblasts preferentially occur in transcribed regions of the genome

Within the Williston basin, thickness variations of the Prairie Formation are common and are interpreted to originate by two processes: differential accumulation of salt during deposition and differential removal of salt by dissolution. Unambiguous evidence for each process is rare because the Prairie/Winnipegosis interval is seldom cored within the US portion of the basin. Therefore, indirect methods, using well logs, provide the principal method for identifying characteristics of the two processes. The results of this study indicate that the two processes can be distinguished using correlations within the Prairie Formation. Several regionally correlative brining-upward and probably shoaling-upward sequences occur within the Prairie Formation. Near the basin center, the lowermost sequence is transitional with the underlying Winnipegosis Formation. This transition is characterized by thinly laminated basal carbonates that become increasingly ...

1988-07-01

203

Computational identification of developmental enhancers:conservation and function of transcription factor binding-site clustersin drosophila melanogaster and drosophila psedoobscura

Full text: DNA rearrangement events leading to chromosomal aberrations are central to ionizing radiation-induced cell death. Although DNA double-strand breaks are probably the lesion that initiates formation of chromosomal aberrations, little is understood about the molecular mechanisms that generate and modulate DNA rearrangement. Examination of the sequences that flank sites of DNA rearrangement may provide information regarding the processes and enzymes involved in rearrangement events. Accordingly, we developed a method using inverse PCR that allows the detection and sequencing of putative radiation-induced DNA rearrangements in defined regions of the human genome. The method can detect single copies of a rearrangement event that has occurred in a particular region of the genome and, therefore, DNA rearrangement detection does not require survival and continued multiplication of the affected cell. Ionizing radiation-induced DNA ...

2003-08-17

204

Targeted integration of baboon endogenous virus in the BEVI locus on human chromosome 6.

Background The identification of sequences that control transcription in metazoans is a major goal of genome analysis. In a previous study, we demonstrated that searching for clusters of predicted transcription factor binding sites could discover active regulatory sequences, and identified 37 regions of the Drosophila melanogaster genome with high densities of predicted binding sites for five transcription factors involved in anterior-posterior embryonic patterning. Nine of these clusters overlapped known enhancers. Here, we report the results of in vivo functional analysis of 27 remaining clusters. Results We generated transgenic flies carrying each cluster attached to a basal promoter and reporter gene, and assayed embryos for reporter gene expression. Six clusters are enhancers of adjacent genes: giant, fushi tarazu, odd-skipped, nubbin, squeeze and pdm2; three drive expression in patterns unrelated to those of neighboring genes; the ...

2004-08-06

205

Origin of complex quantum amplitudes and Feynman's rules

The infection of cultured human cells with baboon endogenous virus (BEV) frequently leads to an association of viral DNA with a specific genetic locus (termed BEVI, for baboon endogenous virus infection) on chromosome 6. Restriction endonuclease digestion of DNA from BEV-infected human cells and their derived somatic cell clones frequently revealed a common cellular DNA sequence in the proximity of one of the junctions between cellular DNA and the integrated virus. We propose that a short cellular DNA sequence, repeated on chromosome 6 and separated by unique DNA sequences, presents a high-affinity target for the integration of BEV in human cells. PMID:6401843

1983-01-13

206

Optimized pulse sequences for the suppression of decoherence in quantum information

Complex numbers are an intrinsic part of the mathematical formalism of quantum theory and are perhaps its most characteristic feature. In this article, we show that the complex nature of the quantum formalism can be derived directly from the assumption that a pair of real numbers is associated with each sequence of measurement outcomes, with the probability of this sequence being a real-valued function of this number pair. By making use of elementary symmetry conditions, and without assuming that these real number pairs have any other algebraic structure, we show that these pairs must be manipulated according to the rules of complex arithmetic. We demonstrate that these complex numbers combine according to Feynman's sum and product rules, with the modulus-squared yielding the probability of a sequence of outcomes.

2010-02-01

207

Molecular Characterization of Coprophilous Fungal Communities Reveals Sequences Related to Root-Associated Fungal Endophytes

The dynamical decoupling (DD) aims at suppressing the decoherence by means of coherent control pulses. Even if devices exist where instantaneous pulses are an adequate approximation, experimentally a finite duration #tau#_p and a bounded amplitude are inevitable. They are the cause of additional errors which can be corrected by designing the pulse shape appropriately. The new pulse has the overall effect of an ideal, instantaneous pulse with the advantage of decoupling the spin (or qubit) from the bath up to the order O(#tau#_p"3). The limitation of the no-go theorem for #pi# pulses is avoided. Hence, the Uhrig sequence (UDD), originally thought for ideal #pi# pulses, works also for bounded control Hamiltonians. Numerical simulations show that concatenated sequences of real pulses are effective against general decoherence.

2010-03-21

208

British Library Electronic Table of Contents (United Kingdom)

This paper reports the use of molecular methods to characterize the coprophilous fungal communities (CFC) that inhabit the dung of four species of mammalian herbivores at two sites, Sevilleta National Wildlife Refuge (SNWR) in New Mexico and Wind Cave National Park (WCNP) in South Dakota. Results reveal that CFC from domesticated cattle (Bos taurus) at SNWR, and bison (Bison bison) and black-tailed prairie dogs (Cynomys ludovicianus) at WCNP were diverse but dominated primarily by members within eight taxonomic orders, including the rarely cultured and anaerobic order Neocallimastigales. In addition, 7.7% (138 of 1,788) of the sequences obtained from all dung samples were at least 97% similar to root-associated fungal (RAF) sequences previously described from blue grama (Bouteloua gracilis...

2011-01-01

209

Identification of mutations leading to the Lesch-Nyhan syndrome by automated direct DNA sequencing of in vitro amplified cDNA

Human cDNA mapping using fluorescence in situ hybridization

The Lesch-Nyhan (LN) syndrome is a severe X chromosome-linked disease that results from a deficiency of the purine salvage enzyme hypoxanthine phosphoribosyltransferase (HPRT). The mutations leading to the disease are heterogeneous and frequently arise as de novo events. The authors have identified nucleotide alterations in 15 independently arising HPRT-deficiency cases by direct DNA sequencing of in vitro amplified HPRT cDNA. They also demonstrate that the direct DNA sequence analysis can be automated, further simplifying the detection of new mutations at this locus. The mutations include DNA base substitutions, small DNA deletions, a single DNA base insertion, and errors in RNA splicing. The application of these procedures allows DNA diagnosis and carrier identification by the direct detection of the mutant alleles within individual families affected by LN.

1989-03-01

210

High-resolution sequence stratigraphy and its controls on uranium mineralization. Taking middle Jurassic Qingtujing formation in Chaoshui basin as an example

Genetic mapping is approached using the techniques of high resolution fluorescence in situ hybridization (FISH). This technology and the results of its application are designed to rapidly generate whole genome as tool box of expressed sequence to speed the identification of human disease genes. The results of this study are intended to dovetail with and to link the results of existing technologies for creating backbone YAC and genetic maps. In the first eight months, this approach generated 60--80% of the expressed sequence map, the remainder expected to be derived through more long-term, labor-intensive, regional chromosomal gene searches or sequencing. The laboratory has made significant progress in the set-up phase, in mapping fetal and adult brain and other cDNAs, in testing a model system for directly linking genetic and physical maps using FISH with small fragments, in setting up a database, and in establishing the ...

1993-03-04

211

Bioinformatic analysis of BBTV satellite DNA in Hainan

By applying the high-resolution sequence stratigraphy, the different sub-divisions of base-level cycles of Qingtujing Formation, Middle Jurassic in Chaoshui Basin are analyzed in detail. 23 short, 3 middle and 1 long base-level cycles are recognized. On the above basis, the corresponding frameworks of high-resolution sequence stratigraphy have been established in northern and southern sub-basins respectively, and the detailed sedimentary facies of MSC_1_-_3 and the special distribution of Qintujing Formation are discussed. It is pointed out that MSC_2 is the most favorable layer for the localization of sandstone-type uranium deposits. (authors)

2006-06-01

212

British Library Electronic Table of Contents (United Kingdom)

Banana bunchy top virus (BBTV), family Nanaviridae, genus Babuvirus, is a single stranded DNA virus (ssDNA) that causes banana bunchy top disease (BBTD) in banana plants. It is the most common and most destructive of all viruses in these plants and is widespread throughout the Asia-Pacific region. In this study we isolated, cloned and sequenced a BBTV sample from Hainan Island, China. The results from sequencing and bioinformatics analysis indicate this isolate represents a satellite DNA component with 12 DNA sequences motifs. We also predicted the physical and chemical properties, structure, signal peptide, phosphorylation, secondary structure, tertiary structure and functional domains of its encoding protein, and compare them with the corresponding quantities in the replication initiatio...

2011-01-01

213

Balance with Unbounded Complexes

Application of integrated high resolution sequence stratigraphy in the Jeanne d`Arc Basin, offshore Newfoundland - an illustration of the systems tracts within the Tithonian to Berriasian Hibernia Sequence

Given a double complex $X$ there are spectral sequences with the $E_2$ terms being either H$_I$ (H$_{II}(X))$ or H$_{II}($H$_I (X))$. But if $H_I(X)=H_{II}(X)=0$ both spectral sequences have all their terms 0. This can happen even though there is nonzero (co)homology of interest associated with $X$. This is frequently the case when dealing with Tate (co)homology. So in this situation the spectral sequences may not give any information about the (co)homology of interest. In this article we give a different way of constructing homology groups of $X$ when H$_I(X)=$H$_{II}(X)=0$. With this result we give a new and elementary proof of balance of Tate homology and cohomology.

2011-01-01

214

Time-Dependent 2D Modeling of Magnetron Plasma Torch in Turbulent Flow

The Jeanne d`Arc Basin is located 320 km offshore in the Grand Banks of Newfoundland. It is a Mesozoic failed-rift basin with recoverable oil reserves of about 2 billion barrels. Its huge Hibernia Field will be in production by late 1997. The basin has been reappraised using sequence stratigraphic concepts, and renewed exploration in the area is expected. The Upper Jurassic - Lower Cretaceous sedimentary accumulation in the Basin was formed by the extensional tectonics that created the North Atlantic Ocean. The sedimentary packages were placed in chronostratigraphic order by calibrating the biostratigraphy of the Oxfordian to Maastrichtian section to the detailed ammonite zonation from the North Sea.

1997-09-01

215

Stability analysis of forced-flow cooled superconducting coil; Numerical simulation on multiple stability. Kyosei reikyaku gata chodendo coil no anteisei kaiseki; Taju anteisei no simulation

A theoretical model is presented to describe the electromagnetic, heat transfer and fluid flow phenomena within a magnetron plasma torch and in the resultant plume, by using a commercial computational fluid dynamics (CFD) code FLUENT. Specific calculations are presented for a pure argon system (i.e., an argon plasma discharging into an argon environment), operated in a turbulent mode. An important finding of this work is that the external axial magnetic field (AMF) may have a significant effect on the behavior of arc plasma and thus affects the resulting plume. The AMF impels the plasma to retract axially and expand radially. As a result, the plasma intensity distribution on the cross section of torch seems to be more uniform. Numerical results also show that with AMF, the highest plasma temperature decreases and the anode arc root moves upstream significantly, while the current density distribution at the anode is more concentrated with a higher peak value. In ...

2008-06-01

216

Solar wind driving of magnetospheric ULF waves: Field line resonances driven by dynamic pressure fluctuations

The stability of a forced-flow cooled superconducting coil is investigated by use of the numerical simulation. The numerical code to integrate the simultaneous partial differential system composed of the 1 D hydrodynamic equations and the 1 D thermal conduction equation has been developed and stability margins are evaluated as functions of coolant mass flow rate, operation current and imposed magnetic field. The results of computations show that the stability margin is multi-valued with respect to these operation parameters, as expected from the experimental results. It is also shown that the appearance of the first unstable regime is closely related to the existance of the stagnant region located at the upstream side of the heated zone and that the second stable regime appears because the heat transfer is appreciably enhanced by the induced backflow due to the thermal expansion of coolant. 13 refs., 13 figs., 1 tab.

1990-06-25

217

River ice jams at bridges

Several observational studies suggest that solar wind dynamic pressure fluctuations can drive magnetospheric ultra-low frequency (ULF) waves on the dayside. To investigate this causal relationship, we present results from Lyon-Fedder-Mobarry (LFM) global, three-dimensional magnetohydrodynamic (MHD) simulations of the solar wind-magnetosphere interaction. These simulations are driven with synthetic solar wind input conditions, where idealized ULF dynamic pressure fluctuations are embedded in the upstream solar wind. In three of the simulations, a monochromatic, sinusoidal ULF oscillation is introduced into the solar wind dynamic pressure time series. In the fourth simulation, a continuum of ULF fluctuations over the 0-50 mHz frequency band is introduced into the solar wind dynamic pressure time series. In this numerical experiment, the idealized solar wind input conditions allow us to study only the effect of a fluctuating solar wind dynamic pressure, while holding ...

2010-01-01

218

Processes and components of cogeneration with gas- and steam turbines; Prozesse und Komponenten der Kraft-Waerme-Kopplung mit Gas- und Dampfturbinen

Ice jamming, known to cause high water levels at even moderate river flows, is described as both the main and least understood source of ice-related bridge damages. This paper describes a joint study by the New Brunswick Department of Transportation, the Department of the Environment, local governments, and the National Water Research Institute, designed to address problems associated with the interaction of ice jams and bridges. The study consists of collecting information at each of four sites in New Brunswick including: historical data on ice jam locations, causes, and water levels; channel bathymetry, width and slope within each study centred at the respective bridge; and documentation of ice conditions throughout the ice season, including measurement of ice cover thickness, observation of breakup mechanisms, times, causes, characteristics and possible impacts of ice jam release. Data analysis will include determination of high stages due to ice jams or surges caused by ...

2000-12-01

219

Performance of the AMS-02 Experiment for High Energy Gamma Ray Astrophysics

The following industrial-scale power station processes are compared with natural gas/light fuel oil heating: Conventional steam generation, steam generation with gas turbine upstream, gas turbine process with low-pressure-waste heat boiler and cogeneration. Industrial gas turbines between 10 and 25 MW are compared with one another; typical boilers and industrial turbines are described. CO{sub 2} and NO{sub x} emissions are indicated. The steam turbines used are the efficient and robust turbines used in industry, wherever possible they are built as single-line plant with the gas turbine. (orig.) [Deutsch] Es werden folgende Industriekraftwerksprozesse mit Erdgas-Leichtoelfeuerung verglichen: Konventioneller Dampfprozess, Dampfprozess mit Vorschaltgasturbine, Gasturbinenprozess mit ND-Abhitzekessel und Kombianlage. Die Industriegasturbinen zwischen 10 und 25 MW werden gegenuebergestellt und typische Kessel und Industrieturbinen vorgestellt. Abschliessend werden ...

1994-12-31

220

New technologies for reservoir management

AMS is a particle detector designed to perform high precision measurements of the cosmic rays fluxes with the main goals of searching for anti-nuclei, as remnants of primordial anti-matter, and of measuring the faintest components of the cosmic flux, anti- protons, positrons and high energy photons. To fulfill the requirements of large acceptance, long exposure time and excellent particle identification needed to achieve the intended results, AMS will operate in space as an attached payload to the International Space Station (ISS), being the first full featured particle physics experiment to operate in the Earth orbit. The AMS-02 accurate measurements of cosmic-ray nuclei, protons, antiprotons, electrons and positrons will be completed by high energy gamma rays detection. The experiment will detect gamma-rays, either by reconstructing e+e? pairs generated by photons converted upstream the tracker (conversion mode), or based on direct identification of ...

2007-01-01

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221

Morusin induces apoptosis and suppresses NF-#kappa#B activity in human colorectal cancer HT-29 cells

The E and P industry has high levels of uncertainty and risk, which oil companies attempt to quantify before embarking on each individual E and P project. However, the actual return on investment often falls well short of the hurdle rate. This discrepancy has been attributed to systemic limitations in decision analysis processes and workflow, which result in repeated underestimation of risk and overestimation of the predicted production of the project. Well designed decision analysis processes in association with clearly defined, multi-scenario analyses of significant technical uncertainties are required. However, simulation of these uncertainties is not realistic given the performance and infrastructure limitations of conventional technology (Begg et al., 2001; Floris and Peersmann, 2000). During the past five years, more than 20 companies have attempted to map the requirements of new technology that is needed to improve uncertainty assessment and decision analysis. Pain points ...

2004-07-01

222

Investigation of the potential influence of production treatment chemicals on produced water toxicity

Morusin is a pure compound isolated from root bark of Morusaustralis (Moraceae). In this study, we demonstrated that morusin significantly inhibited the growth and clonogenicity of human colorectal cancer HT-29 cells. Apoptosis induced by morusin was characterized by accumulation of cells at the sub-G_1 phase, fragmentation of DNA, and condensation of chromatin. Morusin also inhibited the phosphorylation of IKK-#alpha#, IKK-#beta# and I#kappa#B-#alpha#, increased expression of I#kappa#B-#alpha#, and suppressed nuclear translocation of NF-#kappa#B and its DNA binding activity. Dephosphorylation of NF-#kappa#B upstream regulators PI3K, Akt and PDK1 was also displayed. In addition, activation of caspase-8, change of mitochondrial membrane potential, release of cytochrome c and Smac/DIABLO, and activation of caspase-9 and -3 were observed at the early time point. Downregulation in the expression of Ku70 and XIAP was exhibited afterward. Caspase-8 or wide-ranging ...

2008-07-18

223

Hydrologic analysis for ecological risk assessment of watersheds with abandoned mine lands

Production treatment chemicals represent a diverse collection of chemical classes, added at various points from the wellhead to the final flotation cell, to prevent operational upsets and enhance the separation of oil from water. Information in the literature indicates that while many treatment chemicals are thought to partition into oil and not into the produced water, there are cases where a sufficiently water soluble treatment chemical is added at high enough concentrations to suggest that the treatment chemical may add to the aquatic toxicity of the produced water. A study was conducted to evaluate the potential effect of production treatment chemicals on the toxicity of produced waters using the US EPA Seven-day Mysidopsis bahia Survival, Growth and Fecundity Test. Samples of produced water were collected and tested for toxicity from three platforms under normal operating conditions, followed by repeated sampling and testing after a 72-hour period in which treatment chemical usage ...

1993-11-14

224

HSP70, the earliest-induced gene in the zebrafish retina during optic nerve regeneration: Its role in cell survival

As part of on-going study of acid mine drainage (AMD), a comprehensive ecological risk assessment was conducted in the Leading Creek Watershed in southeast Ohio. The watershed is influenced by agriculture and active and abandoned coal-mining operations. This work presents a broad overview of several quantitative measures of hydrology and hydraulic watershed properties available for in risk assessment and evaluates their relation to metrics of ecology. Data analysis included statistical comparisons of metrics of ecology, ecotoxicology, water quality, and physically based parameters describing land use, geomorphology, flow, velocity, and particle size. A multiple regression analysis indicated that abandoned mining operations dominated impacts upon aquatic ecology. It also indicated low flow velocity measurements and a ratio of maximum velocity to average velocity at low flow where helpful in describing variation in macroinvertebrate Total Taxa scores. Other key parameters also identified ...

1999-07-25

225

British Library Electronic Table of Contents (United Kingdom)

Fish retinal ganglion cells (RGCs) can survive and regrow their axons after optic nerve injury. Injured RGCs express anti-apoptotic proteins, such as Bcl-2, after nerve injury; however, upstream effectors of this anti-apoptotic protein are not yet fully understood. Heat shock proteins (HSPs) play a crucial role in cell survival against various stress conditions. In this study, we focused on HSP70 expression in the zebrafish retina after optic nerve injury. HSP70 mRNA and protein levels increased rapidly 2.3-fold in RGCs by 1-6 h after injury and returned to control levels by 1-3 days. HSP70 transcription is regulated by heat shock factor 1 (HSF1). HSF1 mRNA and phosphorylated-HSF1 protein rapidly increased by 2.2-fold in RGCs 0.5-6 h after injury. Intraocular injection of HSP inhibitor I s...

2011-01-01

226

Fuel processing equipment for fuel cell plant. Nenryo denchi plant no nenryo shori sochi

Forced laminar convection in an array of stacked plates

Activated charcoal which is the desulfurizing agent for the fuel cell plant can be used only at the normal temperature, and it must be placed upstream of the fuel gas, increasing the displacement flow of the fuel gas processed in the desulfurizer. In addition, a large quantity of activated charcoal must be filled or the frequency of its exchange must be increased because activated charcoal used at the normal temperature has little sulfur absorbing capacity. This invention is concerned with provision of a desulfurizer in the downstream of the fuel gas compressor of the fuel cell plant to remove sulfur content in the fuel gas by this desulfurizer. As a result, the displacement flow of the processing gas is decreased to increase the processing capacity of the desulfurizing agent. In addition, hydrogen recycling can be eliminated to realize compact arrangement of the equipment and economization of energy consumption. Metal oxide based desulfurizers of copper, iron, ...

1993-02-19

227

Finned fuel element two-phase flow parameter measurements using real-time neutron radiography

A numerical study of laminar flow and heat transfer in an array of stacked rectangular plates is presented. The array is placed in a uniform stream, and the plates are subjected to a constant surface heat flux. This flow configuration is relevant to a number of practical heat transfer devices with finned surfaces. The computations were performed using a finite volume solution of the steady, two-dimensional Navier-Stokes equations and energy equation. A numerical scheme that reduces numerical diffusion is used to discretize the equations. The dominant feature of the flow is the separation, and subsequent reattachment of, the boundary layer, which takes place at Reynolds numbers greater than about 75. The separation first occurs downstream of the leading edge of the plate; then as Re increases, the separation point moves upstream and remains fixed at the leading edge, and the reattachment length increases linearly with Re. The appearance and growth of the separation ...

1994-04-01

228

Finned fuel element two-phase flow parameter measurements using real-time neutron radiography

The measurement of two-phase flow parameters and development of constitutive relationships for the HANARO/MAPLE type finned fuel using Real-Time Neutron Radiography (RTNR) is discussed in this paper. A single element finned Fuel Element Simulator (FES) was used with R134a Freon as the working fluid. To observe the effect of a spacer device on void distribution, single pin tests were performed with and without a spacer. By analyzing the RTNR images using image processing, the effects of the spacer on the time-averaged and instantaneous void fraction distribution were studied. For the experimental results without a spacer, the time-averaged local void distribution is radially asymmetric and the degree of void fluctuation increases with a decreasing frequency along the heated channel, where the observed asymmetry may be caused by flow induced vibration. For the experimental results with a spacer, the spacer clearly limits any significant vibration and the local void distribution becomes ...

2007-04-22

229

Experimental parametric equation for the prediction of valve coefficient (C_v) for choke valve trims

The measurement of two-phase flow parameters for development of constitutive relationships for the HANARO/MAPLE type finned fuel using Real-Time Neutron Radiography (RTNR) is discussed in this paper. A single element finned Fuel Element Simulator (FES) was used with Freon 134a as the working fluid. To observe the effect of a spacer device on void distribution, single pin tests were performed with and without a spacer present. By analyzing the RTNR images using image processing, the effects of the spacer on the time-averaged and instantaneous void fraction distribution were studied. For the experimental results without a spacer, the time-averaged local void distribution is radially asymmetric and the degree of void fluctuation increases with a decreasing frequency along the heated channel, where the observed asymmetry may be caused by flow induced vibration. For the experimental results with a spacer, the spacer clearly limits any significant vibration and the local void distribution ...

2008-02-01

230

Effect of ethanol-diesel blend fuels on emission and particle size distribution in a common-rail direct injection diesel engine with warm-up catalytic converter

The calculation of nominal choke valve size determines the effective capacity for an oil and gas production system. The degree of restriction for the controlling area in the valve is a function of the surrounding geometry. In an orifice plate this is known as the 'velocity of approach' and can be used to determine the meter coefficient (C_m). This paper presents a technique for choke valves, based on the meter velocity of approach parameter, which can be used to predict the Valve Coefficient (C_v) for new trim designs. The prediction method uses a data trend based on a number of flow tests conducted on various trim characteristics. The resultant parametric equation is used to predict the C_v of a new trim geometry. The method relies on experimental data determined per IEC 60534-2-3, with calculations per IEC 60534-2-1. This paper further investigates the effect of varying upstream geometry on C_v for a 4'' nominal valve.

231

Computational study of jet interaction flow field with and without incidence

In this study, the exhaust gas from a common-rail direct injection diesel engine was investigated both upstream and downstream warm-up catalytic converters (WCC). Three different types of ultra-low sulfur fuels (ethanol-diesel blend, ethanol-diesel blend with cetane improver and pure diesel) were tested in this study. The objective of the work was to study the engine performance and the formation of THC (total hydro carbon), CO (carbon monoxide), NO{sub x} (nitrogen oxides), smoke and PM (particulate matters) when using these fuels. THC and CO emissions of the ethanol-diesel blend fuels were slightly increased, and about 50-80% mean conversion efficiencies of THC and CO on catalysts were achieved in the ECE R49 13-mode cycle. Smoke was decreased by more than 42% in the entire ECE 13-mode cycles. From the measurement of scanning mobility particle sizer (SMPS) for the particle size range of 10-385 nm, the total number and total mass of the PM of the ethanol-diesel ...

2008-10-15

232

Clear liquor scrubbing with anhydrite production. Fourth quarter 1996 technical progress report

The objective was to study the interaction of a side jet with the incoming supersonic flow and hypersonic flow. Qualitatively same Cp trends have been obtained as found experimentally. Also in aerodynamic coefficients side jet interaction results in additional pitching moment which is because of the high pressure region in upstream of the jet and a low pressure region in the downstream of the jet. Also jet interaction results in the rise in the lift coefficient. Whereas in the incidence case, simulation has been performed for the hypersonic flows over a biconic body with supersonic lateral jet at Mach 9.7 and incidence of 0"o to incidence of -12"o and 12"o. The results obtained were compared with the experimental and CFD code CFL3D results. PAK-3D over predicts the surface pressure as compared to the CFL3D and experimental results, whereas the qualitative trends are the same. Finally the integrated aerodynamic force coefficients were compared with CFL3D predicted ...

2004-05-09

233

Challenges in environmental monitoring and reporting

This project is part of PETC`s Advanced Power Systems Program, whose mission is to accelerate the commercialization of affordable, high- efficiency, low emission, coal-fueled electric generating technologies. A process concept - Clear Liquor Scrubbing with Anhydrite Production - was proposed and accepted by PETC as a Phase I project in its Mega-PRDA program. the project integrated three process operations - chloride control upstream of the flue gas desulfurization (FGD) system, a clear liquor process for enhanced SO{sub 2} removal performance, and production of anhydrite (anhydrous calcium sulfate) rather than calcium sulfite or gypsum (calcium sulfate dihydrate). The first step in the project was to perform batch and flow-through experiments in the laboratory to confirm theoretical calculations and preliminary laboratory results concerning anhydrite formation, these laboratory experiments were designed to prove that anhydrite can be produced under conditions that ...

1997-02-03

234

A comprehensive approach to cooling tower design

The Canadian Association of Petroleum Producers (CAPP) represents about 170 crude oil and natural gas exploration, development and production companies in regulatory hearings and legal proceedings. Its principal role is to enhance the economic well-being and sustainability of the Canadian upstream petroleum industry. This presentation explores the role that information technology (IT) plays in knowledge and information management and how this role relates to meeting Canada`s target for greenhouse gas emissions reductions set by the Kyoto Protocol. Actions such as the Voluntary Challenge Registry (VCR) and emissions reductions trading are re-visited and the information requirements associated with these actions are highlighted. It is shown that among others, emissions databases between jurisdictions (provincial, national, international) have to be integrated, reduction claims must be tracked, verified and cross-referenced to eliminate double counting, comprehensive ...

1998-12-31

235

narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes.

In this paper, a mathematical model for a counterflow wet cooling tower is derived, which is based on one-dimensional heat and mass balance equations using the measured heat transfer coefficient. The balance equations are solved numerically to predict the temperature change of air and water, as well as the humidity as a function of the cooling tower high. Experimental measurements on two pilot-scale cooling towers were carried out in order to analyze the performance of different cooling tower filling materials. Also, the performance of other cooling tower elements, such as droplet separators and water spray nozzles, was investigated in the pilot experiments. The flow distribution, i.e. the velocity field, upstream to the filling material was predicted using the three-dimensional version of the computational fluid dynamics (CFD) code Fluent/UNS, version 4.2. The calculated flow fields are presented for different distances between the inlet of the air and the filling ...

2001-06-01

236

Worlds within worlds: evolution of the vertebrate gut microbiota

In previous studies it has been established that in Escherichia coli the three known subunits of anaerobic nitrate reductase are encoded by the narGHI operon. From the nucleotide sequence of the narI...Full Text Available

1988-04-01

237

Whole-genome shotgun assembly and comparison of human genome assemblies

Here we use published 16S rRNA gene sequences to compare the bacterial assemblages associated with humans, other mammals, other metazoa, and free-living microbial communities spanning a range...Full Text Available

2008-10-01

238

Whole-genome cancer analysis as an approach to deeper understanding of tumour biology

We report a whole-genome shotgun assembly (called WGSA) of the human genome generated at Celera in 2001. The Celera-generated shotgun data set consisted of 27 million sequencing reads organized in pairs...Full Text Available

2004-02-17

239

Viruses with More Than 1,000 Genes: Mamavirus, a New Acanthamoeba polyphaga mimivirus Strain, and Reannotation of Mimivirus Genes

Recent advances in DNA sequencing technology are providing unprecedented opportunities for comprehensive analysis of cancer genomes, exomes, transcriptomes, as well as epigenomic components. The integration...Full Text Available

2010-01-19

240

Variants within the yeast Ty sequence family encode a class of structurally conserved proteins.

The genome sequence of the Mamavirus, a new Acanthamoeba polyphaga mimivirus strain, is reported. With 1,191,693 nt in length and 1,023 predicted protein-coding genes, the Mamavirus...Full Text Available

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

241

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens

The Ty transposable elements of Saccharomyces cerevisiae form a heterogeneous family within which two broad structural classes (I and II) exist. The two classes differ by two large substitutions and...Full Text Available

1985-06-11

242

Unifying evolutionary and thermodynamic information for RNA folding of multiple alignments

The diagnosis of viral causes of many infectious diseases is difficult due to the inherent sequence diversity of viruses as well as the ongoing emergence of novel viral pathogens, such as SARS coronavirus...Full Text Available

243

Two amyloid states of the prion protein display significantly different folding patterns

Computational methods for determining the secondary structure of RNA sequences from given alignments are currently either based on thermodynamic folding, compensatory base pair substitutions or both....Full Text Available

2008-11-01

244

Transposon-based screens for cancer gene discovery in mouse models

SummaryIt has been well established that a single amino acid sequence can give rise to several conformationally distinct amyloid states. The extent to which amyloid structures...Full Text Available

2010-07-23

245

Transient Hoogsteen Base Pairs in Canonical Duplex DNA

Significant emphasis has recently been placed on the characterization of the human cancer genome. This effort has been assisted by the development of new DNA sequencing technologies that allow...Full Text Available

2010-08-01

246

Transcriptome Profiling of a Toxic Dinoflagellate Reveals a Gene-Rich Protist and a Potential Impact on Gene Expression Due to Bacterial Presence

Sequence-directed variations in the canonical DNA double helix structure that retain Watson-Crick base-pairing play important roles in DNA recognition, topology, and nucleosome positioning....Full Text Available

2011-02-24

247

Transcriptional mapping of the 3' end of the bovine syncytial virus genome.

BackgroundDinoflagellates are unicellular, often photosynthetic protists that play a major role in the dynamics of the Earth's oceans and climate. Sequencing of dinoflagellate nuclear...Full Text Available

248

The use of EPID-measured leaf sequence files for IMRT dose reconstruction in adaptive radiation therapy

The bovine syncytial virus, a member of the retroviral subfamily Spumavirinae, causes a persistent, asymptomatic infection in cattle. Nucleotide sequence analysis of the viral genome revealed two overlapping...Full Text Available

1994-02-01

249

The missing graphical user interface for genomics

For intensity modulated radiation treatment (IMRT) dose reconstruction, multileaf collimator (MLC) log files have been shown applicable for deriving delivered fluence maps. However, MLC log files are...Full Text Available

2008-11-01

250

The adenovirus-2 EIIa early gene promoter: sequences required for efficient in vitro and in vivo transcription.

AbstractThe Galaxy package empowers regular users to perform rich DNA sequence analysis through a much-needed and user-friendly graphical web interface.See research article...Full Text Available

2010-01-01

251

The Specificity of Innate Immune Responses Is Enforced by Repression of Interferon Response Elements by NF-?B p50

A series of deletion mutants extending from -250 toward the capsite has been constructed in the early promoter region of the adenovirus 2 EIIa gene and tested both in vitro, and in vivo after transfection...Full Text Available

1983-10-25

252

The LW blood group glycoprotein is homologous to intercellular adhesion molecules.

The specific binding of transcription factors to cognate sequence elements is thought to be critical for the generation of specific gene expression programs. Members of the nuclear factor κB...Full Text Available

253

The European sea bass Dicentrarchus labrax genome puzzle: comparative BAC-mapping and low coverage shotgun sequencing

The LW blood group antigens reside on a 42-kDa erythrocyte membrane glycoprotein that was purified by immunoaffinity and partially sequenced. From this information, a specific PCR-amplified DNA fragment...Full Text Available

1994-06-07

254

The Application of High Resolution Sequence Stratigraphy for Understanding the Hydrocarbon Prospectivity of the Moere and Southern Voering Basins

BackgroundFood supply from the ocean is constrained by the shortage of domesticated and selected fish. Development of genomic models of economically important fishes should assist...Full Text Available

255

Ternary Complex Formation on the Adenovirus Packaging Sequence by the IVa2 and L4 22-Kilodalton Proteins?

The deep water parts of the Moere and Southern Voering Basins are large frontier areas, which are considered to contain significant undiscovered hydrocarbon resources, within Cretaceous and Paleogene reservoirs. PGS Reservoir AS have evaluated the Cretaceous and Paleogene successions of the shallow and deep water areas offshore Mid-Norway using high-resolution sequence stratigraphic techniques. The successions have been subdivided into about 20 stratigraphic sequences. The results are: (1) A more acurate and higher resolution stratigraphy, (2) Greater constraints on basin palaeoenvironmental and palaeogeographic reconstructions, (4) Fully integrated sandstone fairway models, with increased ability to reservoir and seal quality and continuity, (5) In addition to several obvious giant domal traps, subtler yet significant structural and stratigraphic leads and prospects can be identified, (6) The timing, volume and phase of hydrocarbon generation ...

1999-07-01

256

Subject-specific, multiscale simulation of electrophysiology: a software pipeline for image-based models and application examples

Assembly of infectious adenovirus particles requires seven functionally redundant elements at the left end of the genome, termed A repeats, that direct packaging of the DNA. Previous studies revealed...Full Text Available

2007-11-01

257

Studying bacterial transcriptomes using RNA-seq

Many simulation studies in biomedicine are based on a similar sequence of processing steps, starting from images and running through geometric model generation, assignment of tissue properties, numerical...Full Text Available

2009-06-13

258

Stretched DNA Investigated Using Molecular-Dynamics and Quantum-Mechanical Calculations

Genome-wide studies of bacterial gene expression are shifting from microarray technology to second generation sequencing platforms. RNA-seq has a number of advantages over hybridization-based techniques,...Full Text Available

2010-10-01

259

Strategies for the uses of lanthanide NMR shift probes in the determination of protein structure in solutio. Application to the EF calcium binding site of carp parvalbumin.

AbstractWe combined atomistic molecular-dynamics simulations with quantum-mechanical calculations to investigate the sequence dependence of the stretching behavior of duplex DNA. Our...Full Text Available

2010-01-06

260

Straightforward and de Novo Peptide Sequencing by MALDI-MS/MS Using a Lys-N Metalloendopeptidase*S?

The homologous sequences observed for many calcium binding proteins such as parvalbumin, troponin C, the myosin light chains, and calmodulin has lead to the hypothesis that these proteins have homologous...Full Text Available

1980-10-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

261

Statistical properties of nucleotide clusters in DNA sequences*

In this work, we explore the potential of the metalloendopeptidase Lys-N for MALDI-MS/MS proteomics applications. Initially we digested a HEK293 cellular lysate with Lys-N and, for comparison, in parallel...Full Text Available

2009-04-01

262

Sequence variants of the DFNB31 gene among Usher syndrome patients of diverse origin

Using the complete genome of Plasmodium falciparum 3D7 which has 14 chromosomes as an example, we have examined the distribution functions for the amount of C or G and A or T consecutively...Full Text Available

2005-05-01

263

Sequence and expression of the zebrafish alpha-actinin gene family reveals conservation and diversification among vertebrates

PurposeIt has been demonstrated that mutations in deafness, autosomal recessive 31 (DFNB31), the gene encoding whirlin, is responsible for nonsyndromic hearing loss...Full Text Available

264

Sequence Reactivation in the Hippocampus is Impaired in Aged Rats

alpha-actinins are actin microfilament crosslinking proteins. Vertebrate actinins fall into two classes: the broadly-expressed actinins 1 and 4 (actn1 and actn4)...Full Text Available

2009-11-01

265

Sequence Analysis and Characterization of a Transferable Hybrid Plasmid Encoding Multidrug Resistance and Enabling Zoonotic Potential for Extraintestinal Escherichia coli?

The hippocampus is thought to coordinate memory consolidation by reactivating traces from behavioral experience when the brain is not actively processing new input. In fact, during slow-wave...Full Text Available

2008-07-30

266

Selection and characterization of ricin toxin A-chain mutations in Saccharomyces cerevisiae.

ColV plasmids of extraintestinal pathogenic Escherichia coli (ExPEC) encode a variety of fitness and virulence factors and have long been associated with septicemia and avian colibacillosis....Full Text Available

2010-05-01

267

Role of Conserved Salt Bridges in Homeodomain Stability and DNA Binding*

A DNA sequence encoding the A chain of ricin toxin (RTA) from the castor bean plant, Ricinus communis, was placed under GAL1 promoter control and transformed into Saccharomyces cerevisiae. Induction...Full Text Available

1989-02-01

268

Remobilization of Tol2 transposons in Xenopus tropicalis

The sequence information available for homeodomains reveals that salt bridges connecting pairs 19/30, 31/42, and 17/52 are frequent, whereas aliphatic residues at these sites are rare and mainly restricted...Full Text Available

2009-08-28

269

Relationship between mRNA secondary structure and sequence variability in Chloroplast genes: possible life history implications

BackgroundThe Class II DNA transposons are mobile genetic elements that move DNA sequence from one position in the genome to another. We have previously demonstrated that the naturally...Full Text Available

270

Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae.

BackgroundSynonymous sites are freer to vary because of redundancy in genetic code. Messenger RNA secondary structure restricts this freedom, as revealed by previous findings in...Full Text Available

271

Reduced-Median-Network Analysis of Complete Mitochondrial DNA Coding-Region Sequences for the Major African, Asian, and European Haplogroups

The DUR3 gene, which encodes a component required for active transport of urea in Saccharomyces cerevisiae, has been isolated, and its sequence has been determined. The deduced DUR3 protein profile...Full Text Available

1993-08-01

272

Red tides in the Gulf of Mexico: Where, when, and why?

The evolution of the human mitochondrial genome is characterized by the emergence of ethnically distinct lineages or haplogroups. Nine European, seven Asian (including Native American), and three African...Full Text Available

2002-05-01

273

Rational design of DNA sequences for nanotechnology, microarrays and molecular computers using Eulerian graphs

[1] Independent data from the Gulf of Mexico are used to develop and test the hypothesis that the same sequence of physical and ecological events each year allows the toxic dinoflagellate Karenia...Full Text Available

2006-11-07

274

Rapid, sensitive detection of Mycoplasma pneumoniae in simulated clinical specimens by DNA amplification.

Nucleic acids are molecules of choice for both established and emerging nanoscale technologies. These technologies benefit from large functional densities of ‘DNA processing elements’...Full Text Available

2004-01-01

275

Rapid technique of DNA-DNA in situ hybridisation on formalin fixed tissue sections using microwave irradiation.

The polymerase chain reaction (PCR) was investigated as a means of diagnosing Mycoplasma pneumoniae infections. The target DNA sequence was a 375-bp segment of the P1 virulence protein. This DNA segment...Full Text Available

1992-12-01

276

Rab protein evolution and the history of the eukaryotic endomembrane system

The relative sensitivities of different protocols for detecting cytomegalovirus nucleic acid sequences in histological specimens, using a biotinylated cDNA probe, were assessed. Several commonly used...Full Text Available

1987-08-01

277

Programmed Cell Death during Pollination-Induced Petal Senescence in Petunia1

Spectacular increases in the quantity of sequence data genome have facilitated major advances in eukaryotic comparative genomics. By exploiting homology with classical model organisms, this makes possible...Full Text Available

2010-10-01

278

Primary and secondary structure of the pore-forming peptide of pathogenic Entamoeba histolytica.

Petal senescence, one type of programmed cell death (PCD) in plants, is a genetically controlled sequence of events comprising its final developmental stage. We characterized the pollination-induced...Full Text Available

2000-04-01

279

Prediction method abstracts

A pore-forming peptide is implicated in the potent cytolytic activity of pathogenic Entamoeba histolytica. Using NH2-terminal sequence information of this peptide, the corresponding cDNA was isolated....Full Text Available

1992-10-01

280

Population genetic analysis of large sequence polymorphisms in Plasmodium falciparum blood-stage antigens

This conference was held December 4--8, 1994 in Asilomar, California. The purpose of this meeting was to provide a forum for exchange of state-of-the-art information concerning the prediction of protein structure. Attention if focused on the following: comparative modeling; sequence to fold assignment; and ab initio folding.

1994-12-31

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

281

Phylogeny of Mitochondrial DNA Macrohaplogroup N in India, Based on Complete Sequencing: Implications for the Peopling of South Asia

Plasmodium falciparum, the causative agent of human malaria, invades host erythrocytes using several proteins on the surface of the invasive merozoite, which have been proposed...Full Text Available

2010-03-01

282

Phylogenetic Network for European mtDNA

To resolve the phylogeny of the autochthonous mitochondrial DNA (mtDNA) haplogroups of India and determine the relationship between the Indian and western Eurasian mtDNA pools more precisely, a diverse...Full Text Available

2004-12-01

283

Photocatalytic probing of DNA sequence by using TiO{sub 2}/dopamine-DNA triads.

The sequence in the first hypervariable segment (HVS-I) of the control region has been used as a source of evolutionary information in most phylogenetic analyses of mtDNA. Population genetic inference...Full Text Available

2001-06-01

284

Phosphoglycerate kinase and triosephosphate isomerase from the hyperthermophilic bacterium Thermotoga maritima form a covalent bifunctional enzyme complex.

A method to control charge transfer reaction in DNA using hybrid nanometer-sized TiO{sub 2} nanoparticles was developed. In this system extended charge separation reflects the sequence of DNA and was measured using metallic silver deposition or by photocurrent response. Light-induced extended charge separation in these systems was found to be dependent on the DNA-bridge length and sequence. The yield of photocatalytic deposition of silver was studied in systems having GG accepting sites imbedded in AT runs at varying distances from the TiO{sub 2} nanoparticle surface. Weak distance dependence of charge separation indicative of a hole hopping through mediating adenine (A) sites was found. The quantum yield of silver deposition in the system having a GG accepting site placed 8.5 {angstrom} from the nanoparticle surface was found to be {Phi} = 0.70 (70%) and {Phi} = 0.56 (56%) for (A){sub n} and (AT){sub n/2} bridge, respectively. Hole injection ...

2007-10-15

285

Perspective on post-menopausal osteoporosis: establishing an interdisciplinary understanding of the sequence of events from the molecular level to whole bone fractures

Phosphoglycerate kinase (PGK) from the hyperthermophilic bacterium Thermotoga maritima has been purified to homogeneity. A second larger enzyme with PGK activity and identical N-terminal sequence was...Full Text Available

1995-02-01

286

Personalized Epigenomic Signatures That Are Stable Over Time and Covary with Body Mass Index

Current drug treatments for post-menopausal osteoporosis cannot eliminate bone fractures, possibly because the mechanisms responsible for bone loss are not fully understood. Although research within...Full Text Available

2010-03-06

287

Palindromic Sequence Plays a Critical Role in Human Foamy Virus Dimerization

The epigenome consists of non–sequence-based modifications, such as DNA methylation, that are heritable during cell division and that may affect normal phenotypes and predisposition...Full Text Available

2010-09-15

288

Negative Subtraction Hybridization: An efficient method to isolate large numbers of condition-specific cDNAs

The retroviral RNA genome is dimeric, consisting of two identical strands of RNA linked near their 5′ ends by a dimer linkage structure. Previously it was shown that human foamy virus (HFV)...Full Text Available

2001-04-01

289

Mutational analysis of bacteriophage lambda lysis gene S.

BackgroundThe construction of cDNA libraries is a useful tool to understand gene expression in organisms under different conditions, but random sequencing of unbiased cDNA collections...Full Text Available

290

Molecular dynamics of a ?B DNA element: base flipping via cross-strand intercalative stacking in a microsecond-scale simulation

A plasmid carrying the bacteriophage lambda lysis genes under lac control was subjected to hydroxylamine mutagenesis, and mutations eliminating the host lethality of the S gene were selected. DNA sequence...Full Text Available

1986-09-01

291

Molecular characterization of a Chinese variant of the Flury-LEP strain

The sequence-dependent structural variability and conformational dynamics of DNA play pivotal roles in many biological milieus, such as in the site-specific binding of transcription factors to target...Full Text Available

2008-09-01

292

Molecular Variability of Pseudallescheria boydii, a Neurotropic Opportunist

The entire genome of rabies virus vaccine strain Flury-LEP-C, a Chinese variant of the rabies virus vaccine strain Flury-LEP, was sequenced. The overall length of the genome of Flury-LEP-C strain was...Full Text Available

293

Molecular DNA Systematic Analyses of East Asian Mammals: Sequence Variation of Cytochrome b Gene and Control Region of Mitochondrial DNA of Common Otter, Lutra lutra lutra L. (Mammalia, Carnivora) from Korea

The sequences of the internal transcribed spacer (ITS) ribosomal DNA (rDNA) domain data obtained by restriction fragment length polymorphism analysis with 18S rDNA and fingerprinting (M13) for clinical...Full Text Available

2000-09-01

294

MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms

Full Text Available

2004-09-01

295

Methanogen Diversity Evidenced by Molecular Characterization of Methyl Coenzyme M Reductase A (mcrA) Genes in Hydrothermal Sediments of the Guaymas Basin

MicroRNAs (miRNAs) are endogenously encoded small noncoding RNAs, derived by processing of short RNA hairpins, that can inhibit the translation of mRNAs bearing partially complementary target sequences....Full Text Available

2003-08-19

296

MRI features of epidural extramedullary hematopoiesis

The methanogenic community in hydrothermally active sediments of Guaymas Basin (Gulf of California, Mexico) was analyzed by PCR amplification, cloning, and sequencing of methyl coenzyme M reductase...Full Text Available

2005-08-01

297

Localized mutagenesis of the tetracycline promoter region in pBR322 by 4,5',8-trimethylpsoralen

A case of {beta}-thalassemia intermedia with spinal cord compression due to extramedullary hematopoiesis, which was successfully treated by blood transfusion, is presented. Emphasis was made on the MRI appearance of extramedullary hematopoiesis on different pulse sequences. The theories that aimed to explain the involvement of the epidural space by extramedullary hematopoiesis are discussed.

2000-07-01

298

Light-Dependent Regulation of Cyanobacterial Phytochrome Expression

In vitro mutagenesis of functional DNA gene fragments by covalently reactive agents permits one in principle to examine the consequent alterations in DNA sequence directly. I have carried out selective mutagenesis of the tetracycline resistance gene in the plasmid pBR322 using the long wavelength UV light activated reaction of 4,5',8-trimethylpsoralen (TMP). The mutagenized DNA sequence was the EcoR1-Hind III restriction fragment in the vicinity of the Tcsup(R) promoter. Two classes of mutants were obtained. One exhibited a high level of Tc resistance (40-60 ..mu..g/ml) but still lower than the wild-type. Interestingly, these showed no sequence alterations at all in the vicinity of the TMP-reacted fragment. The other class of mutants exhibited low levels of drug resistance (< 20 ..mu..g/ml) and two of those that were sequenced were found to contain a 15-base pair insertion to the right of ...

1982-04-01

299

Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands.

A histidine kinase protein (Cph1) with sequence homology and spectral characteristics very similar to those of the plant phytochrome has been recently identified in the cyanobacterium Synechocystis...Full Text Available

2000-01-01

300

Investigation of xenotropic murine leukemia virus-related virus (XMRV) in human and other cell lines.

The isolation of an untriakontapeptide from camel pituitary extracts has been described. Its structure has been determined and shown to be identical to the sequence of carboxyl-terminal 31 amino acids...Full Text Available

1976-04-01

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301

Interactions in multiple schedules: negative induction with squirrel monkeys1

Xenotropic murine leukemia virus-related virus (XMRV) was discovered in human prostate tumors and later in some chronic fatigue syndrome (CFS) patients. However, subsequent studies have identified various sources of potential contamination with XMRV and other murine leukemia virus (MLV)-related sequences in test samples. Biological and nucleotide sequence analysis indicates that XMRV is distinct from known xenotropic MLVs and has a broad host range and cell tropism including human cells. Therefore, it is prudent to minimize the risk of human exposure to infection by evaluating XMRV contamination in cell lines handled in laboratory research and particularly those used in the manufacture of biological products. Nested DNA PCR assays were optimized for investigating XMRV gag and env sequences in various cell lines, which included MRC-5, Vero, HEK-293, MDCK, HeLa, and A549, that may be used in the development of some vaccines ...

2011-10-11

302

Insights into Genome Plasticity and Pathogenicity of the Plant Pathogenic Bacterium Xanthomonas campestris pv. vesicatoria Revealed by the Complete Genome Sequence

In Experiment I, lever pressing by squirrel monkeys was maintained under a sequence of variable-interval, multiple variable-interval variable-interval, and multiple variable-interval extinction schedules...Full Text Available

1978-11-01

303

Inferring transcription factor complexes from ChIP-seq data

The gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria is the causative agent of bacterial spot disease in pepper and tomato plants, which leads to economically...Full Text Available

2005-11-01

304

Inferring Stabilizing Mutations from Protein Phylogenies: Application to Influenza Hemagglutinin

Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) allows researchers to determine the genome-wide binding locations of individual transcription factors (TFs) at high resolution....Full Text Available

2011-08-01

305

Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation

One selection pressure shaping sequence evolution is the requirement that a protein fold with sufficient stability to perform its biological functions. We present...Full Text Available

2009-04-01

306

Identification of novel DNA repair proteins via primary sequence, secondary structure, and homology

The vaginal bacterial microbiota of 19 premenopausal women was examined by PCR-denaturing gradient gel electrophoresis (DGGE) and sequencing of the V2-V3 region of the 16S rRNA gene. Ten of the women...Full Text Available

2003-01-01

307

Ia-antigen-T-cell interactions for a thymus-independent antigen composed of D amino acids.

BackgroundDNA repair is the general term for the collection of critical mechanisms which repair many forms of DNA damage such as methylation or ionizing radiation. DNA repair has...Full Text Available

308

IRIS Simplified LERF Model

Synthetic polypeptide antigens of L amino acids, although bearing repeating sequences, are thymus-dependent (L-TD), whereas the same polymers composed of D amino acids are thymus-independent (D-TI),...Full Text Available

1993-02-01

309

Human spumaretrovirus-related sequences in the DNA of leukocytes from patients with Graves disease.

Westinghouse is currently conducting the pre-application licensing of the International Reactor Innovative and Secure (IRIS). One of the key aspects of the IRIS design is its safety-by-designTM philosophy and within this framework the PRA is being used as an integral part of the design process. The most ambitious risk-related goal for IRIS is to reduce the Emergency Planning Zone (EPZ) to within the exclusion area by demonstrating that the off-site doses are consistent with the US Protective Action Guidelines (PAGs) for initiation of emergency response so that the required protective actions would be limited to the exclusion area. As a first step, a model has been developed to provide a first order approximation of the Large Early Release Frequency (LERF) as a surrogate predictor of the off-site doses. A key-aspect of the LERF model development is the characterization of the possible paths of release. Four main categories have been historically pointed out: (1) Core Damage (CD ) ...

2004-10-06

310

Human Pol II promoter recognition based on primary sequences and free energy of dinucleotides

Viruses, and more particularly retroviruses, have been postulated to play a role in the pathogenesis of autoimmune diseases. In a search for spumaretrovirus infection markers, we screened a group of...Full Text Available

1992-11-01

311

How DNA coiling enhances target localization by proteins

BackgroundPromoter region plays an important role in determining where the transcription of a particular gene should be initiated. Computational prediction of eukaryotic Pol II promoter...Full Text Available

312

High-resolution sequence stratigraphic character and sandstone-type uranium ore formation. A case from Saihan Formation in Baiyinwula area, Erlian Basin

Many genetic processes depend on proteins interacting with specific sequences on DNA. Despite the large excess of nonspecific DNA in the cell, proteins can locate their targets rapidly. After initial...Full Text Available

2008-10-14

313

Heterogeneous mutations in the human lipoprotein lipase gene in patients with familial lipoprotein lipase deficiency.

High-resolution sequence stratigraphy has been applied widely in the petroleum exploration and development, many achievements have been achieved. However, it is in the beginning stage that high-resolution sequence stratigraphy is applied to explore the sandstone-type uranium deposits in Erlian Basin. By applying principles of high-resolution sequence stratigraphy and taking typical boreholes as an example, sedimentary cycles of Saihan Formation, the ore-bearing formation in Baiyinwula area are divided and correlated through cross sections. One long-term cycle (LSC_1), two middle-term cycles (MSC_1, MSC_2) have been identified in this study. Based on this and combined with the mineralization character of sandstone uranium deposits in this area, it is presented that the interlayer oxidation zone is developed mainly in the rising hemicycle of MSC_1 and uranium ore bodies predominantly in channel sand bodies that were developed ...

2006-12-01

314

Genomic cloning and characterization of a ricin gene from Ricinus communis.

The DNA sequences were determined for the lipoprotein lipase (LPL) gene from five unrelated Japanese patients with familial LPL deficiency. The results demonstrated that all five patients are homozygotes...Full Text Available

1991-12-01

315

Genomic Diversity and Evolution of Mycobacterium ulcerans Revealed by Next-Generation Sequencing

A genomic clone that specifies a single polypeptide precursor for ricin, a toxic lectin of Ricinus communis (castor bean), was isolated, sequenced and Sl mapped. The gene encodes a 64 kDa precursor...Full Text Available

1985-11-25

316

Genome-wide characterization of simple sequence repeats in cucumber (Cucumis sativus L.)

Mycobacterium ulcerans is the causative agent of Buruli ulcer, the third most common mycobacterial disease after tuberculosis and leprosy. It is an emerging infectious disease that...Full Text Available

2009-09-01

317

Genome Sequence of Campylobacter jejuni strain 327, a strain isolated from a turkey slaughterhouse

BackgroundCucumber, Cucumis sativus L. is an important vegetable crop worldwide. Until very recently, cucumber genetic and genomic resources, especially molecular...Full Text Available

318

Gene expression profiling of oxidative stress response of C. elegans aging defective AMPK mutants using massively parallel transcriptome sequencing

Campylobacter is one of the leading causes of food-borne gastroenteritis and has a high prevalence in poultry. Campylobacter jejuni subsp. jejuni 327 is a subspecies...Full Text Available

319

GLUE-IT and PEDEL-AA: new programmes for analyzing protein diversity in randomized libraries

BackgroundA strong association between stress resistance and longevity in multicellular organisms has been established as many mutations that extend lifespan also show increased...Full Text Available

320

Full genome gene expression analysis of the heat stress response in Drosophila melanogaster

There are many methods for introducing random mutations into nucleic acid sequences. Previously, we described a suite of programmes for estimating the completeness and diversity of randomized DNA libraries...Full Text Available

2008-07-01

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321

Fragment condensation of peptides on teflon with radiationally grafted polystyrene

The availability of full genome sequences has allowed the construction of microarrays, with which screening of the full genome for changes in gene expression is possible. This method can provide a wealth...Full Text Available

2005-10-01

322

Fragment condensation of peptides on teflon with radiationally grafted polystyrene

In the synthesis of the peptidyl-polymer corresponding to the sequence (57-100) of the ..beta.. chain of human hemoglobin on Teflon with radiationally grafted polystyrene a comparative study was made of different methods of fragment condensation. It was shown that the most effective methods are azide condensation and condensation with the use of complex F.

1986-12-10

323

First report of the complete sequence of Sida golden yellow vein virus from Jamaica.

In the synthesis of the peptidyl-polymer corresponding to the sequence (57-100) of the #beta# chain of human hemoglobin on Teflon with radiationally grafted polystyrene a comparative study was made of different methods of fragment condensation. It was shown that the most effective methods are azide condensation and condensation with the use of complex F.

324

Exome sequencing identifies GRIN2A as frequently mutated in melanoma

Begomoviruses are phytopathogens that threaten food security [18]. Sida spp. are ubiquitous weed species found in Jamaica. Sida samples were collected island-wide, DNA was extracted via a modified Dellaporta method, and the viral genome was amplified using degenerate and sequence-specific primers [2, 11]. The amplicons were cloned and sequenced. Sequence analysis revealed that a DNA-A molecule isolated from a plant in Liguanea, St. Andrew, was 90.9% similar to Sida golden yellow vein virus-[United States of America:Homestead:A11], making it a strain of SiGYVV. It was named Sida golden yellow vein virus-[Jamaica:Liguanea 2:2008] (SiGYVV-[JM:Lig2:08]). The cognate DNA-B, previously unreported, was successfully cloned and was most similar to that of Malvastrum yellow mosaic Jamaica virus (MaYMJV). Phylogenetic analysis suggested that this virus was most closely related to begomoviruses that infect malvaceous hosts in Jamaica, ...

2011-05-29

325

Evidence for nonrandom hydrophobicity structures in protein chains.

The incidence of melanoma is increasing more than any other cancer, and knowledge of its genetic alterations is limited. To systematically analyze such alterations, we performed whole-exome...Full Text Available

2011-05-01

326

Escherichia coli MW005: lambda Red-mediated recombineering and copy-number induction of oriV-equipped constructs in a single host

The question of whether proteins originate from random sequences of amino acids is addressed. A statistical analysis is performed in terms of blocked and random walk values formed by binary hydrophobic...Full Text Available

1996-09-03

327

Efficient discovery of ASCL1 regulatory sequences through transgene pooling

BackgroundEscherichia coli strain EL350 contains chromosomally integrated phage lambda Red recombinase genes enabling this strain to be used for modifying the sequence...Full Text Available

328

Detection of genetic mutations in repetitive DNA sequences in somatic human cells after low-dose irradiation; Nachweis genetischer Veraenderungen in repetitiven DNA-Sequenzen in somatischen menschlichen Zellen nach Bestrahlung im niedrigen Dosisbereich

Zebrafish transgenesis is a powerful and increasingly common strategy to assay vertebrate transcriptional regulatory control. Several challenges remain, however, to the broader application of...Full Text Available

2010-06-01

329

Detecting Retroviral Sequences in Chronic Fatigue Syndrome

No abstract prepared

1998-03-01

330

DNA Display I. Sequence-Encoded Routing of DNA Populations

XMRV or xenotropic murine leukemia virus-related retrovirus, a recently discovered retrovirus, has been linked to both prostate cancer and chronic fatigue syndrome (CFS). Recently, the teams of Drs....Full Text Available

331

Complexity of the microRNA repertoire revealed by next-generation sequencing

Recently reported technologies for DNA-directed organic synthesis and for DNA computing rely on routing DNA populations through complex networks. The reduction of these ideas to practice has been limited...Full Text Available

2004-07-01

332

Complete sequence of the chicken glyceraldehyde-3-phosphate dehydrogenase gene.

MicroRNAs (miRNAs) have been implicated to play key roles in normal physiological functions, and altered expression of specific miRNAs has been associated with a number of diseases. It is of great interest...Full Text Available

2010-11-01

333

Complete genome sequence of Actinosynnema mirum type strain (101T)

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), an evolutionarily conserved glycolytic enzyme, is constitutively expressed in most cell types yet is induced to high levels during the development of...Full Text Available

1985-03-01

334

Complete characterization of the edited transcriptome of the mitochondrion of Physarum polycephalum using deep sequencing of RNA

Actinosynnema mirum Hasegawa et al. 1978 is the type species of the genus, and is of phylogenetic interest because of its central phylogenetic location in the Actino-synnemataceae, a rapidly growing family within the actinobacterial suborder Pseudo-nocardineae. A. mirum is characterized by its motile spores borne on synnemata and as a producer of nocardicin antibiotics. It is capable of growing aerobically and under a moderate CO2 atmosphere. The strain is a Gram-positive, aerial and substrate mycelium producing bacterium, originally isolated from a grass blade collected from the Raritan River, New Jersey. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first complete genome sequence of a member of the family Actinosynnemataceae, and only the second sequence from the actinobacterial suborder Pseudonocardineae. The 8,248,144 bp long single replicon genome ...

2009-05-20

335

Complete Sequencing of pNDM-HK Encoding NDM-1 Carbapenemase from a Multidrug-Resistant Escherichia coli Strain Isolated in Hong Kong

RNAs transcribed from the mitochondrial genome of Physarum polycephalum are heavily edited. The most prevalent editing event is the insertion of single Cs, with Us and dinucleotides...Full Text Available

2011-08-01

336

Coat protein gene duplication in a filamentous RNA virus of plants.

BackgroundThe emergence of plasmid-mediated carbapenemases, such as NDM-1 in Enterobacteriaceae is a major public health issue. Since they mediate resistance to...Full Text Available

337

Clostridium perfringens Delta Toxin Is Sequence Related to Beta Toxin, NetB, and Staphylococcus Pore-Forming Toxins, but Shows Functional Differences

Computer-assisted analysis revealed a striking sequence similarity between the putative 24-kDa protein (p24) encoded by open reading frame (ORF) 5 of beet yellows closterovirus and the coat protein...Full Text Available

1992-10-01

338

Cloning of an insecticidal cholesterol oxidase gene and its expression in bacteria and in plant protoplasts.

Clostridium perfringens produces numerous toxins, which are responsible for severe diseases in man and animals. Delta toxin is one of the three hemolysins released by a number of C....Full Text Available

339

Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22

We cloned and sequenced structural gene choM, which encodes an insecticidally active cholesterol oxidase in Streptomyces sp. strain A19249. The primary translation product was predicted to be a 547-amino-acid...Full Text Available

1994-12-01

340

Cloning and sequencing of a dehalogenase gene encoding an enzyme with hydrolase activity involved in the degradation of gamma-hexachlorocyclohexane in Pseudomonas paucimobilis.

Two overlapping cDNA clones encoding human DNA topoisomerase II were identified by two independent methods. In one, a human cDNA library in phage {lambda} was screened by hybridization with a mixed oligonucleotide probe encoding a stretch of seven amino acids found in yeast and Drosophila DNA topoisomerase II; in the other, a different human cDNA library in a {lambda}gt11 expression vector was screened for the expression of antigenic determinants that are recognized by rabbit antibodies specific to human DNA topoisomerase II. The entire coding sequences of the human DNA topoisomerase II gene were determined from these and several additional clones, identified through the use of the cloned human TOP2 gene sequences as probes. Hybridization between the cloned sequences and mRNA and genomic DNA indicates that the human enzyme is encoded by a single-copy gene. The location of the gene was mapped to chromosome 17q21-22 by in ...

1988-10-01

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341

Cloning and Sequencing of a Novel meta-Cleavage Dioxygenase Gene Whose Product Is Involved in Degradation of ?-Hexachlorocyclohexane in Sphingomonas paucimobilis

In Pseudomonas paucimobilis UT26, gamma-hexachlorocyclohexane (gamma-HCH) is converted by two steps of dehydrochlorination to a chemically unstable intermediate, 1,3,4,6-tetrachloro-1,4-cyclohexadiene...Full Text Available

1993-10-01

342

Cloning and Sequencing of a 2,5-Dichlorohydroquinone Reductive Dehalogenase Gene Whose Product Is Involved in Degradation of ?-Hexachlorocyclohexane by Sphingomonas paucimobilis

Sphingomonas (formerly Pseudomonas) paucimobilis UT26 utilizes γ-hexachlorocyclohexane (γ-HCH), a halogenated organic insecticide,...Full Text Available

1999-11-01

343

Cloning and Nucleotide Sequence Determination of the Entire mec DNA of Pre-Methicillin-Resistant Staphylococcus aureus N315

Sphingomonas (formerly Pseudomonas) paucimobilis UT26 utilizes γ-hexachlorocyclohexane (γ-HCH), a halogenated organic insecticide,...Full Text Available

1998-03-01

344

Chromosome location of Oryza sativa recombination linkage groups.

In methicillin-resistant Staphylococcus aureus, the methicillin resistance gene mecA is localized within a large chromosomal region which is absent in the methicillin-susceptible...Full Text Available

1999-06-01

345

Chiropteran types I and II interferon genes inferred from genome sequencing traces by a statistical gene-family assembler

In situ hybridization, a powerful tool for the molecular cytogeneticist, can be used to physically map repetitive, low-copy, and unique DNA sequences in plant chromosomes. With the availability of a...Full Text Available

1992-09-15

346

Chemiosmotic model for plant mitochondria

BackgroundThe rate of emergence of human pathogens is steadily increasing; most of these novel agents originate in wildlife. Bats, remarkably, are the natural reservoirs of many...Full Text Available

347

Characterization of the H(+)-pumping F1F0 ATPase of Vibrio alginolyticus.

A balanced application of chemiosmosis to plant mitochondria is presented. Electron transport carriers are sequenced to achieve proton translocation across the inner membrane. The processes of proton translocation, use, and release are integrated to yield a description of the effective proton gradient available for metabolite transport and ATP synthesis. 27 references, 6 figures.

1983-12-01

348

Characteristics of the molecular diversity of the outer membrane protein A gene of Haemophilus parasuis

The F1F0 ATPase of Vibrio alginolyticus was cloned from a chromosomal lambda library. The unc operon, which contains the structural genes for the ATPase, was sequenced and shown to have a gene organization...Full Text Available

1990-12-01

349

CLOTU: An online pipeline for processing and clustering of 454 amplicon reads into OTUs followed by taxonomic annotation

The molecular diversity of the gene encoding the outer membrane protein A (OmpA) of Haemophilus parasuis has been unclear. In this study, the structural characteristics, sequence types,...Full Text Available

2010-07-01

350

Breaking the 1000-gene barrier for Mimivirus using ultra-deep genome and transcriptome sequencing

BackgroundThe implementation of high throughput sequencing for exploring biodiversity poses high demands on bioinformatics applications for automated data processing. Here we introduce...Full Text Available

351

Biophysical characterization of recombinant proteins: A key to higher structural genomics success

BackgroundMimivirus, a giant dsDNA virus infecting Acanthamoeba, is the prototype of the mimiviridae family, the latest addition to the family of the nucleocytoplasmic...Full Text Available

352

Behavioral genomics of honeybee foraging and nest defense

Hundreds of genomes have been successfully sequenced to date, and the data are publicly available. At the same time, the advances in large-scale expression and purification of recombinant proteins have...Full Text Available

2010-10-01

353

Avian Nephritis Virus (ANV) as a New Member of the Family Astroviridae and Construction of Infectious ANV cDNA

The honeybee has been the most important insect species for study of social behavior. The recently released draft genomic sequence for the bee will accelerate honeybee behavioral genetics. Although...Full Text Available

2007-04-01

354

Applications and Experience with PCR-Based Assays to Predict Blood Group Antigens

The complete RNA genome of the avian nephritis virus (ANV) associated with acute nephritis in chickens has been molecularly cloned and sequenced. Excluding the poly(A) tail, the genome comprises 6,927...Full Text Available

2000-09-01

355

Application of quantitative sedimentology in the characterization of subsurface reservoirs and outcrop analogs

SummaryDNA-based tests are increasingly being used to predict a blood group phenotype. This is possible because genes encoding 29 of the 30 blood group systems have been cloned and sequenced,...Full Text Available

2009-01-01

356

Antigenic analysis of the second extra-cellular loop of the human beta-adrenergic receptors.

The thesis deals with methods of describing the reservoir rock in central oil and gas fields on the Norwegian continental shelf. Methods of enhancing the level of recovery are dealt with. Following topics are given: Sand body dimensions and infill sequences of stable, humid-climate delta plain channels; minipermeameter-based study of permeability trends in channel sand bodies; significance of tidal cyclicity for modelling of reservoir heterogeneities in the lower Jurassic Tilje Formation, mid-Norwegian shelf; geometry and facies of large-scale flow units in fluvial-dominated fan-delta-front sequences; quantified fluvial architecture in ephemeral stream deposits of the Esplugafreda Formation (Palaeocene), Tremp-Graus Basin, northern Spain; the Safari project - collection and storage of field analogue data for quantitative reservoir modelling; sedimentary architecture of field analogues for reservoir information (SAFARI): a case study of the ...

1994-12-31

357

An Aggregate Dynamic Stochastic Model for an Air Traffic System

Polyclonal antibodies were raised in rabbits by immunization with free peptides corresponding to positions 197-222 of the human beta 1-adrenergic receptor (beta 1 peptide) and the corresponding sequence...Full Text Available

1989-10-01

358

Amino acid analysis at the picomole level. Application to the C-terminal sequence analysis of polypeptides.

processes, we construct a stochastic dynamic model for air- craft counts in ... Also , queueing models for the arrival of aircraft at ... A queueing model has also been used to study ...... Assignment and Aircraft-Sequencing Algorithms in Terminal ...

359

Acousto-optic multiplexing and demultiplexing

Amino acids labelled with dimethylaminoazobenzenesulphonyl chloride can be separated by reversed-phase high-pressure liquid chromatography and detected in the visible region (436 nm). All 19 naturally...Full Text Available

1981-12-01

360

ARISE: American renaissance in science education

A system is claimed for multiplexing or demultiplexing pulsed laser radiation having an acousto-optical device which is electrically controlled to switch a common path of high pulse rate laser radiation between a plurality of spatially distinct paths for relatively lower pulse rate laser radiation at which the pulses are sequenced according to a predetermined time pattern. The acousto-optical element typically includes a Bragg cell which is electrically driven by a set of distinct frequencies, causing deflection of radiation passing therethrough at a predetermined set of angles whereby pulsed radiation on a single path may be distributed onto the plural separate paths or radiation on plural separate paths of time-sequenced pulses of radiation can be combined into a single path of augmented pulse rate. The control of the acousto-optical element may be provided by selectively switching the output of a plurality of fixed frequency oscillators of ...

1980-06-03

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361

A structural determinant required for RNA editing

The national standards and state derivatives must be reinforced by models of curricular reform. In this paper, ARISE presents one model based on a set of principles--coherence, integration of the sciences, movement from concrete ideas to abstract ones, inquiry, connection and application, sequencing that is responsive to how people learn.

1998-09-14

362

A post-labeling method for multiplexed and multicolored genotyping analysis of SSR, indel and SNP markers in single tube with bar-coded split tag (BStag)

RNA editing by adenosine deaminases acting on RNAs (ADARs) can be both specific and non-specific, depending on the substrate. Specific editing of particular adenosines may depend on the overall sequence...Full Text Available

2011-07-01

363

A new perspective on phylogeny and evolution of tetraodontiform fishes (Pisces: Acanthopterygii) based on whole mitochondrial genome sequences: Basal ecological diversification?

BackgroundGenotyping analysis using capillary DNA sequencing with fluorescently labeled primer pairs obtained by polymerase chain reaction (PCR) is widely used, but is expensive....Full Text Available

364

A new application of MRT of the lung using ultra-shot turbo spin echo sequences (UTSE)

BackgroundThe order Tetraodontiformes consists of approximately 429 species of fishes in nine families. Members of the order exhibit striking morphological diversity and radiated...Full Text Available

365

A multistep bioinformatic approach detects putative regulatory elements in gene promoters

T_2 weighted ultra-short turbo spin echo sequences were used in five individuals with variations in echo times, delayed triggering and echo intervals. To reduce movement artifacts all examinations were carried out with ECG and respiratory triggering. The sequences giving optimal image quality were then employed in 19 patients having various pulmonary abnormalities. Image resolutions, artifacts, image contrasts and diagnostic value were then judged by two observers and compared with CT. In the first study, a diastole-triggered UTSE sequence with the shortest echo proved optimal (T_E=90 ms, T_R=2-4 s, echo=9 ms, turbo factor=19). In the patient series studied, MRT was inferior to CT with regard to resolution and number of artifacts, but better in respect of contrast and diagnostic value. Using UTSE of the lung, MRT can produce images of good quality. Compared with CT, contrast is better with MRT, offering diagnostic ...

366

A microarray analysis of the rice transcriptome and its comparison to Arabidopsis

BackgroundSearching for approximate patterns in large promoter sequences frequently produces an exceedingly high numbers of results. Our aim was to exploit biological knowledge for...Full Text Available

367

A hypothetical model for the peptide binding domain of hsp70 based on the peptide binding domain of HLA.

Arabidopsis and rice are the only two model plants whose finished phase genome sequence has been completed. Here we report the construction of an oligomer microarray based on the presently...Full Text Available

2005-09-01

368

A Marker-Dense Physical Map of the Bradyrhizobium japonicum Genome

The sequences of the peptide binding domains of 33 70 kd heat shock proteins (hsp70) have been aligned and a consensus secondary structure has been deduced. Individual members showed no significant...Full Text Available

1991-05-01

369

A DNA transposon-based approach to validate oncogenic mutations in the mouse

Bacterial artificial chromosome (BAC) clones are effective mapping and sequencing reagents for use with a wide variety of small and large genomes. This report describes the development of a physical...Full Text Available

2001-08-01

370

Three-frame Algorithm of Car Path Reconstruction from Airborne Traffic Data

Large-scale cancer genome projects will soon be able to sequence many cancer genomes to comprehensively identify genetic changes in human cancer. Genome-wide association studies have also identified...Full Text Available

2008-12-16

371

The nucleotide sequence of a major species of leucine tRNA from bovine liver.

The airborne traffic monitoring system forms a novel technology of detecting vehicle motion. An optical digital camera located on an airborne platform produces a series of images which then are processed to recognized the fixed vehicles. In this way the video data are converted into the time sequence of frames containing the vehicle coordinates. In the present work a three-frame algorithm is developed to identify the succeeding vehicle positions. It is based on finding the neighboring points in the frame sequence characterized by minimal acceleration. To verify and optimize the developed algorithm a ``Virtual Road'' simulator was created. Finally available empirical data are analyzed using the created algorithm.

2007-01-01

372

The nucleotide sequence and organization of nuclear 5S rRNA genes in yellow lupine

Through the use of a variety of post-labeling techniques, the nucleotide sequence of a major species of leucine tRNA from bovine liver was determined to be pG-G-U-A-G-C-G-U-G-m-G-C-ac-C-G-A-G-C-G-G-D-C-psi-A-A-G-G-C-m-G-C-U-G-G-A-psim-...Full Text Available

1980-02-25

373

Simple high-throughput annotation pipeline (SHAP)

We have isolated a genomic clone containing 'Lupinus luteus' 5S ribosomal RNA genes by screening with 5S rDNA probe clones that were hybridized previously with the initiator methionine tRNA preparation (contaminated) with traces of rRNA or its degradation products). The clone isolated contains ten repeat units of 342 bp with 119 bp fragment showing 100% homology to the 5S rRNA from yellow lupine. Sequence analysis indicates only point heterogeneities among the flanking regions of the genes. (author). 6 refs, 3 figs.

1993-01-01

374

British Library Electronic Table of Contents (United Kingdom)

Summary: SHAP (simple high-throughput annotation pipeline) is a lightweight and scalable sequence annotation pipeline capable of supporting research efforts that generate or utilize large volumes of DNA sequence data. The software provides Grid capable analysis, relational storage and Web-based full-text searching of annotation results. Implemented in Java, SHAP recognizes the limited resources of many smaller research groups. Availability: Source code is freely available under GPLv3 at External Link Not Shown. Contact: matt.demaere@unsw.edu.au; r.cavicchioli@unsw.edu.au

2011-01-01

375

Organizational demands on the man-organization interface in nuclear power plant operation

Multiplicity formulas for a class of representations of affine Kac-Moody algebras

The partial project SR 2039/4 investigates organizational demands and factors of the man-organization interface. Thee study is divided into the sections inventory of rules and guidelines; evaluation of organizational structures and modes of operation, and working out of demand characteristics and evaluation criteria. The objective consists in representing, analyzing and evaluating the efficiency structure of sequence organization, and deriving practice-relevant concept proposals in order to optimize the man-organization interface. In this regard, the working out of control mechanisms for the evaluation of organizational sequences is of considerable importance as a kind of working aid. (orig./DG).

1994-04-01

376

Isolation and amino acid sequence of a short-chain neurotoxin from an Australian elapid snake, Pseudechis australis.

Simple recursion formulas are derived for the multiplicities of the dominant weight vectors appearing in a class of irreducible highest weight representations of the indecomposable affine Kac-Moody algebras. This class is characterized by the appearance of exactly two distinct infinite sequences of dominant weight vectors. The general procedure used for the enumeration of these representations and for the derivation of the corresponding multiplicity formulas is that presented by Capps for the analysis of those irreducible representations containing exactly one such infinite sequence. This procedure includes the classification of representations in terms of congruence and the identification of Weyl orbits by the norm of the dominant weight. Some of the results presented have application to physical theories such as string field theories.

1992-01-01

377

Evolution of the primary components of massive binary stars in the case of Roche lobe overflow after main-sequence evolution

A short-chain neurotoxin Pseudechis australis a (toxin Pa a) was isolated from the venom of an Australian elapid snake Pseudechis australis (king brown snake) by sequential chromatography on CM-cellulose, Sephadex G-50 and CM-cellulose columns. Toxin Pa a has an LD50 (intravenous) value of 76 micrograms/kg body wt. in mice and consists of 62 amino acid residues. The amino acid sequence of Pa a shows considerable homology with those of short-chain neurotoxins of elapid snakes, especially of true sea snakes. PMID:4091794

1985-12-01

378

Analysis of the tet repressor-operator interactions using the uracil-DNA glycosylase footprinting system

The components with masses 32 and 64M _s_u_n, evolved with the matter mixing in the semiconvective zone and filling their Roche lobes, after the main-sequence evolution overflow their Roche lobes and lose matter during the first part of the helium core burning. After the overflow end, the components lose the matter by stellar wind. The components shrink in the nuclear timescale. At first, they have the blue supergiant character with anomalous CNO abundance, then - the Wolf-Rayet stars character.

379

A combinatorial spanning tree model for knot Floer homology

The tet repressor regulated expression of the Tn-10-encoded tetracycline resistance determinant in a tetracycline-dependent manner. In the absence of tetracycline, the tet repressor binds as a dimer to the 19-base-pair palindromic tet operator sequence. Amino acid homologies and genetic studies with trans-dominant mutants suggest that sequence-specific recognition of the tet operator involves the extensively studied helix-turn-helix motif. We have used the uracil-DNA glycosylase (UDG) footprinting systems to identify thymine contacts in the tet operator that are essential for the formation of tet repressor-operator complexes.

1994-12-31

380

Sequences, geographic variations and molecular phylogeny of venom phospholipases and threefinger toxins of eastern India Bungarus fasciatus and kinetic analyses of its Pro31 phospholipases A2.

We iterate Manolescu's unoriented skein exact triangle in knot Floer homology with coefficients in the fraction field of the group ring (Z/2Z)[Z]. The result is a spectral sequence which converges to a stabilized version of delta-graded knot Floer homology. The (E_2,d_2) page of this spectral sequence is an algorithmically computable chain complex expressed in terms of spanning trees, and we show that there are no higher differentials. This gives the first combinatorial spanning tree model for knot Floer homology.

2011-01-01

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381

Making sense of theory of mind and paranoia: The psychometric properties and reasoning requirements of a false belief sequencing task.

Eight phospholipases A2 (PLAs) and four three-finger-toxins (3FTx) from the pooled venom of Bungarus fasciatus (Bf) were previously studied and sequenced, but their expression pattern in individual Bf venom and possible geographic variations remained to be investigated. We herein analyze the individual venom of two Bf specimens from Kolkata (designated as KBf) to address this question. Seven PLAs and five 3FTx were purified from the KBf venoms, and respective cDNAs were cloned from venom glands of one of the snakes. Comparison of their mass and N-terminal sequence revealed that all the PLAs were conserved in both KBf venoms, but that two of their 3FTx isoforms were variable. When comparing the sequences of these KBf-PLAs with those published, only one was found to be identical to that of Bf Vb-2, and the other five were 94-98% identical to those of Bf II, III, Va, VI and XI-2, respectively. Notably, the most abundant PLA ...

2006-12-08

382

Estimation of source term release during SGTR sequences at Wolsong plants

Introduction. This study used Item-Response Theory (IRT) to model the psychometric properties of a false belief picture sequencing task. Consistent with the mental time travel hypothesis of paranoia, we anticipated that performance on this deductive theory of mind (ToM) task would not be associated with the presence of persecutory delusions but would be related to other clinical, cognitive, and demographic factors. Method. A large (N=237) and diverse clinical and nonclinical sample differing in levels of depression and paranoid ideation performed 2 ToM tasks: the false belief sequencing task and a ToM stories task that was used to assess the validity of the false belief sequencing task as a measure of ToM. Results. A unidimensional IRT model was found to fit the data well. Latent ToM ability as measured by the false belief sequencing task was negatively related with age and positively with IQ. In ...

2011-08-15

383

Applications of high resolution sequence stratigraphy in North Sea syn-rift reservoir correlation and development

Source term release characteristics are analyzed for the severe SGTR (Steam Generator Tube Rupture) sequences beyond the design basis accidents in Wolsong 2/3/4 plants which are of CANDU6 type reactor. In PWRs, SGTR sequences have long been recognized to be important and are distinctly different from the non-bypass sequences since there is a direct fission product release path from the primary system to the environment bypassing the containment gas volume. Meanwhile, a SGTR in a CANDU reactor is analyzed not to provide a complete and direct path into the environment for the source term resulting from a severe accident. This is because the majority of the fission product released arises from heatup and interactions of the disassembled fuel channel segments and debris in the calandria tank rather than from fuel heatup in the fuel channel. These fission products are released from the calandria tank into the containment ...

1998-10-21

384

Transcriptional regulation in Drosophila: the post-genome challenge.

Tectonically active basins may host a spectrum of sequence stratigraphic expressions previously considered to be spatially mutually exclusive. In low accommodation areas with high sediment supply, fourth order eustatic cyclicity results in high frequency sequence sets while within rapidly subsiding areas, time-equivalent Type-2 sequences are expressed by highly asymmetrical coarsening upward successions, resembling large parasequences. In the shallow marine Fulmar Formation, of the U.K. North Sea Central Graben a sequence boundary and overlying lowstand deposits, which illustrate the effects of laterally variable subsidence rate and intrabasinal topography on the expression of a eustatic sea-level fall, lie between the Glosense and Serratum (J54a and J54b) maximum flooding surfaces. The syn-rift physiography comprises major tilted fault blocks, with the Central Graben dipping parallel to the major ...

1995-08-01

385

The application of high resolution sequence stratigraphy to reservoir zonation and management of the Beryl Field

Drosophila melanogaster has long been at the forefront of studies of transcriptional regulation in animals. Many fundamental ideas--such as cis control elements that act over long distances, the regulation of development by hierarchical cascades of transcription factors, dosage compensation, and position effect variegation--originated from studies of the fruit fly. The recent completion of the euchromatic DNA sequence of Drosophila is another breakthrough. The sequence data highlight important unanswered questions. For example, only one-fifth of the 124 Mb of Drosophila euchromatic DNA codes for protein. The function of the remaining 100 Mb of mostly unique DNA is largely unknown. Some proportion of this non-reading frame DNA must encode the functional recognition sites targeted by the approximately 700 sequence-specific DNA binding proteins that regulate transcription in Drosophila, but what proportion? Most or very ...

2001-03-01

386

Structural determinants of alpha-bungarotoxin binding to the sequence segment 181-200 of the muscle nicotinic acetylcholine receptor #alpha# subunit: Effects of cysteine/cystine modification and species-specific amino acid substitution

Tidally dominated sections from the Middle Jurassic Beryl Formation of the Beryl Field, Viking Graben (UKCS) were deposited during rapid changes in relative sea level. High density well coverage in this key producing interval has allowed the construction of an integrated high resolution sequence stratigraphic scheme based on sedimentological, ichnofaunal, wireline, biostratigraphic and engineering data. From this database a number of high resolution sequences in the Bajocian-Bathonian Beryl Formation have been identified composed of interbedded sandstones, siltstones and mudstones. These sediments formed tidally-influenced deltas and estuaries deposited in actively subsiding half-grabens during regionally extensive base-level changes. For reservoir management purposes, the formation has been split into 5 units. The thickest interval, Unit 3, is considered here. In the northern part of the Beryl Field, Unit 3 is bounded above and below by ...

1996-08-01

387

Microbial dynamics in upflow anaerobic sludge blanket (UASB) bioreactor granules in response to short-term changes in substrate feed

The sequence segment 181-200 of the Torpedo nicotinic acetylcholine receptor (nAChR) #alpha#subunit forms a binding site for #alpha#-bungarotoxin (#alpha#-BTX). Synthetic peptides corresponding to the homologous sequences of human, calf, mouse, chicken, frog, and cobra muscle nAChR #alpha#1 subunits were tested for their ability to bind "1"2"5I-#alpha#-BTX, and differences in #alpha#-BTX affinity were determined by using solution (IC_5_0s) and solid-phase (K_ds) assays. Panels of overlapping peptides corresponding to the complete #alpha#1 subunit of mouse and human were also tested for #alpha#-BTX binding, but other sequence segments forming the #alpha#-BTX site were not consistently detectable. The role of a putative vicinal disulfide bound between Cys-192 and -193, relative to the Torpedo sequence, was determined by modifying the peptides with sulfhydryl reagents. Reduction and alkylation of the ...

388

Hypercapnic normalization of BOLD fMRI: comparison across field strengths and pulse sequences.

The complexity and diversity of the microbial communities in biogranules from an upflow anaerobic sludge blanket (UASB) bioreactor were determined in response to short-term changes in substrate feeds. The reactor was fed simulated brewery wastewater (SBWW) (70% ethanol, 15% acetate, 15% propionate) for 1.5 months (phase 1), acetate / sulfate for 2 months (phase 2), acetate-alone for 3 months (phase 3), and then a return to SBWW for 2 months (phase 4). Performance of the reactor remained relatively stable throughout the experiment as shown by COD removal and gas production. 16S rDNA, methanogen-associated mcrA and sulfate reducer-associated dsrAB genes were PCR amplified, then cloned and sequenced. Sequence analysis of 16S clone libraries showed a relatively simple community composed mainly of the methanogenic Archaea (Methanobacterium and Methanosaeta), members of the Green Non-Sulfur (Chloroflexi) group of Bacteria, followed by fewer numbers ...

2010-08-01

389

DEFF Research Database (Denmark)

The blood oxygenation level-dependent (BOLD) functional magnetic resonance imaging (fMRI) signal response to neural stimulation is influenced by many factors that are unrelated to the stimulus. These factors are physiological, such as the resting venous cerebral blood volume (CBV(v)) and vessel size, as well as experimental, such as pulse sequence and static magnetic field strength (B(0)). Thus, it is difficult to compare task-induced fMRI signals across subjects, field strengths, and pulse sequences. This problem can be overcome by normalizing the neural activity-induced BOLD fMRI response by a global hypercapnia-induced BOLD signal. To demonstrate the effectiveness of the BOLD normalization approach, gradient-echo BOLD fMRI at 1.5, 4, and 7 T and spin-echo BOLD fMRI at 4 T were performed in human subjects. For neural stimulation, subjects performed sequential finger movements at 2 Hz, while for global stimulation, subjects breathed a 5% CO(2) ...

2004-01-01

390

High resolution sequence stratigraphy in the upper Jurassic carbonate platform of the Paris basin: Reservoir geometries in lagoonal, protected marine and ooids shoals environments

Evidence for the presence of two supracrustal sequences in the central Wind River Mountain, Wyoming

Reservoir geometry reconstruction within carbonate platforms has been carried out in the Upper Jurassic of the Paris Basin based on high-resolution sequence stratigraphy correlations (cores and well-logs). 18 cored wells over 25 kM{sup 2} have been correlated (Saint-Clair-sur-Epte field, Gaz de France). During lower and middle Oxfordian the Paris Basin is a carbonate platform prograding southward. This progradational trend can be subdivided into three upper order sequences (parasequences sets, mean duration: one m.y.). The lower parasequence-set is a stacking of shallowing-upward parasequences made up of tidal ooids bars surrounded laterally and backward by more protected environments (large variety of coated grains). The middle parasequence-set is recorded in lagoonal environments with numerous channels and lobes of storm washover-fans. Metric-thick sequences have been correlated over the field. They are ernersive upward ...

1995-08-01

391

Comparison of turbo inversion recovery magnitude (TIRM) with T2-weighted turbo spin-echo and T1-weighted spin-echo MR imaging in the early diagnosis of acute osteomyelitis in children

Supracrustal rocks, although volumetrically minor, are found throughout the Archean basement of the central and northern Wind River Mountains. Detailed mapping in the Medina Mountain area suggests that at least two discrete sedimentation events are preserved. The older sequence occurs as melanosomes in a multiple deformed migmatitic gneiss. Rock types include mafic rocks (metavolcanics.), calc-silicates, iron formation and rare pelites. Although retrogression is widespread, small patches with granulite mineralogies are found preserved. The younger supracrustal sequence consists of banded amphibolites, calc-silicates, semipelitic and pelitic gneiss. These rocks form synformal structures that are up to 4 km in length. The coherent nature of these rocks and the lack of the aforementioned porphyritic dikes strongly suggests that this sequence, the Medina Mountain. Supracrustals (MMS) is considerably younger than the ...

1985-01-01

392

Comparing compressed sequences for faster nucleotide BLAST searches.

Objective. To compare turbo inversion recovery magnitude (TIRM) with standard T1-weighted (T1-W) and T2-weighted (T2-W) MR sequences in the very early detection of acute osteomyelitis in children. Materials and methods. In 15 children with osteomyelitis, 15 sets of T1-W spin-echo (SE) (TR/TE, 400-640/12-17), T2-W turbo spin-echo (TSE) (TR/TE/ETL, 3290-4465/112-120/11), and TIRM (TR/TE/TI, 4000-6120/60/160) images were acquired with a 1.0-T magnet. Contrast-to-noise (C/N) ratios and percentage of signal between lesion and normal bone marrow were analysed with a computer-assisted image analysing system in a region of interest (ROI). Results. In 13 of 15 patients, the absolute signal enhancement in a ROI on the TIRM images was better than on the T1-W SE and T2-W TSE images and in 14 of 15 cases, C/N ratios were also better on the TIRM images than on the other sequences. In the other cases, the TIRM signal was diagnostically equivalent. On the TIRM ...

1998-11-01

393

Application of sequence stratigraphy to carbonate reservoir prediction, Early Palaeozoic eastern Warburton basin, South Australia

Molecular biologists, geneticists, and other life scientists use the BLAST homology search package as their first step for discovery of information about unknown or poorly annotated genomic sequences. There are two main variants of BLAST: BLASTP for searching protein collections and BLASTN for nucleotide collections. Surprisingly, BLASTN has had very little attention; for example, the algorithms it uses do not follow those described in the 1997 BLAST paper and no exact description has been published. It is important that BLASTN is state-of-the-art: Nucleotide collections such as GenBank dwarf the protein collections in size, they double in size almost yearly, and they take many minutes to search on modern general purpose workstations. This paper proposes significant improvements to the BLASTN algorithms. Each of our schemes is based on compressed bytepacked formats that allow queries and collection sequences to be compared four bases at a time, ...

394

The evolution of habitable zones during stellar lifetimes and its implications on the search for extraterrestrial life

The Early Palaeozoic Warburton Basin underlies the gas and oil producing Cooper and Eromanga Basins. Postdepositional tectonism created high potential fracture porosities, complicating the stratigraphy and making reservoir prediction difficult. Sequence stratigraphy integrating core, cuttings, well-log, seismic and biostratigraphic data has recognized a carbonate-dominated to mixed carbonate/siliciclastic supersequence comprising several depositional sequences. Biostratigraphy based on trilobites and conodonts ensures reliable well and seismic correlations across structurally complex areas. Lithofacies interpretation indicates sedimentary environments ranging from carbonate inner shelf, peritidal, shelf edge, deep outer shelf and slope to basin. Log facies show gradually upward shallowing trends or abrupt changes indicating possible sequence boundaries. With essential depositional models and sequence ...

1996-12-31

395

The diagnosis of inflammatory muscular and vascular conditions using MRT with STIR sequences

A stellar evolution computer model has been used to determine changes in the luminosity L and effective temperature T(e) of single stars during their time on the main sequence. The range of stellar masses investigated was from 0.5 to 1.5 times that of the Sun, each with a mass fraction of metals (metallicity, Z) from 0.008 to 0.05. The extent of each star's habitable zone (HZ) has been determined from its values of L and T(e). These stars form a reference framework for other main sequence stars. All of the 104 main sequence stars known to have one or more giant planets have been matched to their nearest stellar counterpart in the framework, in terms of mass and metallicity, hence closely approximating their HZ limits. The limits of HZ, for each of these stars, have been compared to its giant planet(s)'s range of strong gravitational influence. This allows a quick assessment as to whether Earth-mass planets could exist in ...

2003-01-01

396

The Secondary Stars of Cataclysmic Variables

The role of MRT in the prebiopsy diagnosis of muscular and vascular inflammatory conditions was evaluated prospectively and an optimal method of examination was investigated. 92 patients with a suspected diagnosis of myositis (60 cases) or vasculitis (32 cases) were examined, in each case two extremities were studied using transverse T_1 and T_2 weighted SE sequences and double echo STIR sequences on a 0.5 Tesla (56 patients) or 1.5 Tesla magnet (36 patients; T5/S15 Gyroscan, Philips). The site of the biopsy depended on the MRT findings. In 41 patients the suspected diagnosis was confirmed histologically, in two patients an infective myositis was diagnosed on clinical grounds despite negative histology. MRT demonstrated muscle oedema in 86% of patients. There were negative findings after immuno-suppressive therapy (two patients), in focal myositis (3 out of 4 patients) and in one of 7 patients with untreated vasculitis. Amongst 49 patients in ...

397

Smooth muscle myosin heavy chain locus (MYH11) maps to 16p13. 13-p13. 12 and establishes a new region of conserved synteny between human 16p and mouse 16

I review what we know about the donor stars in cataclysmic variables (CVs), focusing particularly on the close link between these binary components and the overall secular evolution of CVs. I begin with a brief overview of the "standard model" of CV evolution and explain why the key observables this model is designed to explain - the period gap and the period minimum -- are intimately connected to the properties of the secondary stars in these systems. CV donors are expected to be slightly inflated relative to isolated, equal-mass main-sequence (MS) stars, and this "donor bloating" has now been confirmed observationally. The empirical donor mass-radius relationship also shows a discontinuity at M_2 = 0.2 M_sun which neatly separates long- and short-period CVs. This is strong confirmation of the basic disrupted magnetic braking scenario for CV evolution. The empirical M_2-R_2 relation can be combined with stellar models to construct a complete, semi-empirical donor ...

2011-01-01

398

Rearrangement of a common cellular DNA domain on chromosome 4 in human primary liver tumors

The human smooth muscle myosin heavy chain locus (MYH11) was mapped by fluorescence in situ hybridization to the middle of the p arm of chromosome 16 using a genomic cosmid clone containing coding sequences of the gene as probe. Probe from coding sequence, when applied to Southern blots of a panel of hybrids containing different portions of human chromosome 16, localized the gene to 16p13.13-13.12. Coding sequence PCR primers, when used on the DNA from a CHO-mouse hybrid clone mapping panel informative for mouse chromosomes, showed that the gene was located on mouse chromosome 16. These results correct a recent assignment of MYH11 from 16q12.2 to the region of the 16p-arm inversion breakpoint seen in acute myelomonocytic leukemia (AMML) M4Eo and demonstrate that the conflicting data do not result from the presence of additional MYH genes on the q arm of the chromosome. Also, a new region of conserved synteny between human ...

1993-10-01

399

Rearrangement of a common cellular DNA domain on chromosome 4 in human primary liver tumors

Hepatitis B virus (HBV) DNA integration has been shown to occur frequently in human hepatocellular carcinomas. The authors have investigated whether common cellular DNA domains might be rearranged, possibly by HBV integration, in human primary liver tumors. Unique cellular DNA sequences adjacent to an HBV integration site were isolated from a patient with hepatitis B surface antigen-positive hepatocellular carcinoma. These probes detected rearrangement of this cellular region of chromosomal DNA in 3 of 50 additional primary liver tumors studied. Of these three tumor samples, two contained HBV DNA, without an apparent link between the viral DNA and the rearranged allele; HBV DNA sequences were not detected in the third tumor sample. By use of a panel of somatic cell hybrids, these unique cellular DNA sequences were shown to be located on chromosome 4. Therefore, this region of chromosomal DNA might be implicated in the ...

1988-02-01

400

Physical mapping of human chromosome 16. Annual progress report

Hepatitis B virus (HBV) DNA integration has been shown to occur frequently in human hepatocellular carcinomas. The authors have investigated whether common cellular DNA domains might be rearranged, possibly by HBV integration, in human primary liver tumors. Unique cellular DNA sequences adjacent to an HBV integration site were isolated from a patient with hepatitis B surface antigen-positive hepatocellular carcinoma. These probes detected rearrangement of this cellular region of chromosomal DNA in 3 of 50 additional primary liver tumors studied. Of these three tumor samples, two contained HBV DNA, without an apparent link between the viral DNA and the rearranged allele; HBV DNA sequences were not detected in the third tumor sample. By use of a panel of somatic cell hybrids, these unique cellular DNA sequences were shown to be located on chromosome 4. Therefore, this region of chromosomal DNA might be implicated in the ...

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401

PROPERTIES OF DISKS AND BULGES OF SPIRAL AND LENTICULAR GALAXIES IN THE SLOAN DIGITAL SKY SURVEY

We aim to isolate cDNAs mapping to human chromosome 16 and localise such cDNAs on the high resolution physical map. In collaboration with LANL, PCR primers will be synthesised from cDNA sequences mapped to chromosome 16 and used as ESTs in the generation of mega-YAC contigs for this chromosome. Probing of high density cosmid grids will enable integration of the ESTs into cosmid contigs and location of the cosmid contigs on the YAC contig. A hn-cDNA library has been constructed from the hybrid CY18 which contains chromosome 16 as the only human chromosome. A modified screening protocol has been successfully developed and 15 hn-cDNA clones have been sequenced and localised on the hybrid map. Sequence analysis of four of these revealed that they were known cDNAs, which are now mapped to chromosome 16. Development of techniques to allow the isolation of longer cDNAs from the identified exons is in progress. This will depend on ...

1993-08-01

402

Molecular cloning of the human homeobox gene goosecoid (GSC) and mapping of the gene to human chromosome 14q32. 1

A bulge-disk decomposition is made for 737 spiral and lenticular galaxies drawn from a Sloan Digital Sky Survey galaxy sample for which morphological types are estimated. We carry out the bulge-disk decomposition using the growth curve fitting method. It is found that bulge properties, effective radius, effective surface brightness, and also absolute magnitude, change systematically with the morphological sequence; from early to late types, the size becomes somewhat larger, and surface brightness and luminosity fainter. In contrast, disks are nearly universal, their properties remaining similar among disk galaxies irrespective of detailed morphologies from S0 to Sc. While these tendencies were often discussed in previous studies, the present study confirms them based on a large homogeneous magnitude-limited field galaxy sample with morphological types estimated. The systematic change of bulge-to-total luminosity ratio, B/T, along the morphological ...

2009-11-01

403

Magnetic resonance imaging in patients with heart valve prostheses

Goosecoid is a homeobox gene first isolated from a Xenopus dorsal lip cDNA library. Homologous genes have been isolated from mouse, zebrafish, and chick. In all species examined, the gene is expressed and plays an important role during the process of gastrulation in early embryonic development. The authors report here the cloning of the human goosecoid (GSC) from a genomic library and the sequence of its encoded protein. The genomic organization and protein sequence of the human gene are highly conserved with respect to those of its Xenopus and mouse counterparts: all three genes consist of three exons, with conserved exon-intron boundaries. The sequence of the homeo-domain is 100% conserved in most vertebrates. Using somatic cell hybrid and chromosomal in situ hybridization, the gene was mapped to chromosome 14q32.1. 30 refs., 3 figs., 2 tabs.

1994-05-15

404

MRT versus CT in the diagnosis of pneumonia. Evaluation of a T_2-weighted utrafast turbo-spin-echo sequence (UTSE)

Artifical valve prostheses are often regarded as a contraindication for magnetic resonance imaging (MRI), although preliminary in vitro studies suggested, that patients with these metallic implants might safely undergo MR examination. This study reports on the experience with a group of 89 patients with 100 heart valve prostheses who were examined by spin-echo MR and gradient-echo MR. MR examination was performed in all patients without complications. The spin-echo sequence showed advantages in the depiction of anatomical structures like paravalvular abcesses. Anatomical structures adjacent to the artificial valve were clearly visivle and the metal components of the valves showes no or only small artifacts. Artifacts were accentuated when using gradient-echo sequences. Gradient-echo sequences provided valuable information regarding the presence of valvular insufficiency. Physiological valvular regurgitation was easy to ...

405

MR imaging of bone marrow with gradient echo sequences. Pt. 1. Contrast conditions of phase-identical and opposed-phase gradient echo sequences. Kernspintomographische Screeninguntersuchungen des Knochenmarkes mit Gradientenecho-Sequenzen. T. 1. Kontrastverhaeltnisse phasenidentischer und phasenverschobener Gradientenecho-Sequenzen. Untersuchungen von Probanden und pathologisch-anatomischen Praeparaten

Purpose: To evaluate a T_2-weighted URSE sequence for the assessment of pulmonary infiltrations in comparison to CT. Methods: 28 MRT scans of 22 patients with confirmed pneumonia were recorded on a 1.5 Tesla apparatus with an expiratory and diastolic triggered, T_2-weighted ultrafast-spin-echo sequence in axial slice mode with the following parameters: TR_e_f_f/T_E/Turbofactor 2000-4000/90 ms/21-23; slice thickness/separation 6/0.6 mm; FOV 360 mm; 24 slices. 24 spiral CTs (sice thickness/table advance: 1-2 mm/10 mm) were available for comparison. The separate evaluation of MRTs and CTs was performed by three radiologists in a consensus procedure with regard to pulmonary lesions (e.g., infiltration, round foci, net patterns) and image quality of the MRTs (4-step scale). Results: In 71% of the cases the CTs and MRTs agreed with the diagnosis and representation of the lesions, in 25% MRT was superior. MRT was better for the detection of pulmonary ...

1999-05-01

406

Human cDNA mapping using fluorescence in situ hybridization. Progress report, April 1, 1992--December 31, 1992

Anatomical specimens and normal persons were studied by gradient echo MR imaging to determine the influence of different echo times (TE) on bone marrow contrast. First of all, six normal persons were studied to determine specific echo times for in-phase and opposed-phase states. Using different sequences bone marrow contrast in isolated femoral bones was determined and compared to results of pathological exams. Red bone marrow had no signal on opposed-phase images; contrast between red and yellow marrow was higher on opposed-phase than on in-phase images. Bone marrow lesions can be expected to be visualised with high signal on opposed-phase images; this technique should be especially suited for MR imaging of bone marrow. (orig.).

1991-06-01

407

Fiscal 1998 industrial science and technology R and D project. Research report on R and D of genome informatics technology (Development of stable oil supply measures using complex biosystem); 1998 nendo genome informatics gijutsu kenkyu kaihtsu seika hokokusho. Fukugo seibutsukei riyo sekiyu antei kyokyu taisaku kaihatsu

Genetic mapping is approached using the techniques of high resolution fluorescence in situ hybridization (FISH). This technology and the results of its application are designed to rapidly generate whole genome as tool box of expressed sequence to speed the identification of human disease genes. The results of this study are intended to dovetail with and to link the results of existing technologies for creating backbone YAC and genetic maps. In the first eight months, this approach generated 60--80% of the expressed sequence map, the remainder expected to be derived through more long-term, labor-intensive, regional chromosomal gene searches or sequencing. The laboratory has made significant progress in the set-up phase, in mapping fetal and adult brain and other cDNAs, in testing a model system for directly linking genetic and physical maps using FISH with small fragments, in setting up a database, and in establishing the ...

1993-03-04

408

Efficient Clustering with Limited Distance Information

This report describes the fiscal 1998 result on development of genome informatics technology. As comparative analysis technique of genes, the combination of electrophoresis and PCR was used. For improvement of the throughput and reproducibility of the technique, module- shuffling primers were used, and the multi(96)-arrayed capillary fragment analyzer was devised. The system detecting SNPs rapidly was also developed successfully. As analysis technology of DNA sequence by use of triple- stranded DNA formation, study was made on construction of long cDNA libraries, selective subtraction of specific sequences from libraries, and the basic technology of homologous cloning. Study was also made on each reaction step of IGCR technique for fast analysis, and specifications of a fluorescence transfer monitor. As modeling technique of genetic sequence information, the simulation model was developed for gene expression regulatory ...

1999-03-01

409

Modelling transport of water and solutes in future wetlands in Forsmark

Given a point set S and an unknown metric d on S, we study the problem of efficiently partitioning S into k clusters while querying few distances between the points. In our model we assume that we have access to one versus all queries that given a point s in S return the distances between s and all other points. We show that given a natural assumption about the structure of the instance, we can efficiently find an accurate clustering using only O(k) distance queries. We use our algorithm to cluster proteins by sequence similarity. This setting nicely fits our model because we can use a fast sequence database search program to query a sequence against an entire dataset. We conduct an empirical study that shows that even though we query a small fraction of the distances between the points, we produce clusterings that are close to a desired clustering given by manual classification.

2010-01-01

410

Merger control in the energy sector. An empirical investigation for the Federal Republic of Germany; Fusionskontrolle in der leitungsgebundenen Energiewirtschaft. Eine empirische Untersuchung fuer Deutschland

The Forsmark area consists of a number of natural wetlands. As a part of the evaluation of wetlands in the safety assessment for the area, possible future wetlands are being studied with respect to hydrology and transport mechanisms. A sensitivity analyses is performed to point out the governing parameters for the wetland hydraulics. The analysis of future wetlands is carried out using the hydrological model system Mike SHE. Mike SHE has been used to describe the near-surface hydrology for a regional model area in Forsmark. Three types of areas have been chosen. Today's lake Bolundfjaerden is because of its shallow depth likely to develop into a mire in the future. As it is situated in the downstream part of the regional model area, the runoff to the lake from upstream surface water system is significant. Lake Eckarfjaerden is situated in the upstream part of the catchment at a higher altitude and with a smaller inflow. Lake Puttan is ...

2006-03-15

411

Measurements of actinides in soil, sediments, water and vegetation in Northern New Mexico

In 1998, EU legislation initiated a liberalisation process among European energy markets. The process of market opening has significantly changed the functioning of the markets and the competition conditions of the energy sector. At the same time, the industry witnessed a surge of mergers and far-reaching changes in the structure of German electricity and natural gas markets occurred. Against this background, the doctoral thesis at hand surveys the merger activities in the energy sector as well as the merger control policy of the German Federal Cartel Office during the first years of liberalisation. The study starts with a portrayal of the institutional framework of the German merger control regime and a survey of the relevant economic literature on horizontal and vertical mergers. Subsequent to the legal and theoretical considerations, the merger activities and the merger control policy of the German Federal Cartel Office are investigated empirically. The data set is based on ...

2008-11-06

412

Evaluate Potential Means of Rebuilding Sturgeon Populations in the Snake River between Lower Granite and Hells Canyon Dams, 2001 Annual Report.

This study was undertaken during 1991 - 1998 to identify the origin of plutonium uranium in northern New Mexico Rio Grande and tributary stream sediments. Isotopic fingerprinting techniques help distinguish radioactivity from Los Alamos National Laboratory (LANL) and from global fallout or natural sources. The geographic area covered by the study extended from the headwaters of the Rio Grande in southern Colorado to Elephant Butte Reservoir in southern New Mexico. Over 100 samples of stream channel and reservoir bottom sediments were analyzed for the atom ratios of plutonium and uranium isotopes using thermal ionization mass spectrometry (TIMS). Comparison of these ratios against those for fallout or natural sources allowed for quantification of the Laboratory impact. Of the seven major drainages crossing LANL, movement of LANL plutonium into the Rio Grande can only be traced via Los Alamos Canyon. The majority of sampled locations within and adjacent to LANL have little or no input of ...

2002-01-01

413

Evaluate Potential Means of Rebuilding Sturgeon Populations in the Snake River between Lower Granite and Hells Canyon Dams, 2000 Annual Report.

The specific research goal of this project is to identify means to restore and rebuild the Snake River white sturgeon (Acipenser transmontanus) population to support a sustainable annual subsistence harvest equivalent to 5 kg/ha/yr (CBFWA 1997). Based on data collected, a white sturgeon adaptive management plan will be developed. This 2001 annual report covers the fifth year of sampling of this multi-year study. In 2001 white sturgeon were captured, marked, and population data were collected in the Snake and Salmon rivers. The Snake River was sampled between Lower Granite Dam (rkm 174) and the mouth of the Salmon River (rkm 303), and the Salmon River was sampled from its mouth upstream to Hammer Creek (rkm 84). A total of 45,907 hours of setline effort and 186 hours of hook-and-line effort was employed in 2001. A total of 390 white sturgeon were captured and tagged in the Snake River and 12 in the Salmon River. Since 1997, 36.1 percent of the tagged white sturgeon ...

2003-03-01

414

[Molecular analysis of cytoplasmic male sterility]. Progress report, June 1, 1989--June 15, 1991

The specific research goal of this project is to identify means to restore and rebuild the Snake River white sturgeon (Acipenser transmontanus) population to support a sustainable annual subsistence harvest equivalent to 5 kg/ha/yr (CBFWA 1997). Based on data collected, a white sturgeon adaptive management plan will be developed. This 2000 annual report covers the fourth year of sampling of this multi-year study. In 2000 white sturgeon were captured, marked, and population data were collected in the Snake and Salmon rivers. The Snake River was sampled between Lower Granite Dam (rkm 174) and the mouth of the Salmon River (rkm 303), and the Salmon River was sampled from its mouth upstream to Hammer Creek (rkm 84). A total of 53,277 hours of setline effort and 630 hours of hook-and-line effort was employed in 2000. A total of 538 white sturgeon were captured and tagged in the Snake River and 25 in the Salmon River. Since 1997, 32.8 percent of the tagged white sturgeon ...

2003-03-01

415

Visualization of disturbed flow with spin-echo and cine MR imaging

We have determined the actual RNA sequence of four mitochondrial genes: pcf, nad3, rps12, and coxII. Because plant mitochondrial proteins cannot be predicted from DNA sequences, the actual primary structures of the encoded proteins were unknown. We have gained information concerning the process of RNA editing. Editing can occur before splicing. Our data is consistent with the hypothesis that RNA editing is not simultaneous with transcription. Unlike other systems, the process of editing in plant does not exhibit a discernable direction. As a result of our RNA editing studies, we have produced a number of clones of the proper coding regions tube used for incorporating these mitochondrial genes into the nucleus. 6 refs., 4 figs.

1991-12-31

416

VLF wave stimulation by pulsed electron beams injected from the space shuttle

MR images of steady and pulsatile disturbed flow, obtained with use of flow-compensated spin-echo (SE) and cine pulse sequences, revealed excellent flow visualization in three dimensions. Phantoms, built from molds of actual blood vessels, reproduced laminar, disturbed, or turbulent flow. Video recording (VR), performed under conditions equivalent to those of the MR experiments, showed separation zones identical to those seen on SE images. Pulsatile flow studies showed complex patterns of vortical flow on cine images and VR. Varying pulse sequence details changed contrast but not flow patterns. The validation of MR observations by VR has implications for clinical cine imaging and low abnormal signals observed on MR angiograms.

417

The Morphology, Ontogeny, and Small Subunit rRNA Gene Sequence Analysis of Diophrys parappendiculata n. sp. (Protozoa, Ciliophora, Euplotida), a New Marine Ciliate from Coastal Waters of Southern China

Among the investigations conducted on the space shuttle flight STS 3 March 1982 was an experiment in which a 1-keV, 100-mA electron gun was pulsed at 3.25 and 4.87 kHz. The resultant waves were measured with a broadband plasma wave receiver. At the time of flight the experimental setup was unique in that the electron beam was square wave modulated and that the shuttle offered relatively long times for in situ measurements of the ionospheric plasma response to the VLF pulsing sequences. In addition to electromagnetic response at the pulsing frequencies the waves exhibited various spectral harmonics as well as the unexpected occurrence of satellite lines around those harmonics. Both phenomena occurred with a variety of different characteristics for different pulsing sequences.

418

British Library Electronic Table of Contents (United Kingdom)

ABSTRACT. The morphology, morphogenesis, and phylogeny of Diophrys parappendiculata n. sp., a large marine ciliate isolated from the coastal waters of Daya Bay, southern China, were investigated. This new species is characterized by a combination of its large size, appendiculata-pattern of ciliature, and bipartite adoral zone of membranelles. The main stages of morphogenesis during binary fission were also recorded and described. Comparisons of morphological characteristics with similar congeners support the validity of the new species. The small subunit rRNA gene sequence of D. parappendiculata is 96.3-99.94% similar to those of four other congeners; it differs in four nucleotides from that of Diophrys appendiculata (i.e. structural similarity was 99.94%). Phylogenetic analysis indicates ...

2011-01-01

419

T cell receptor beta chain from sea bream (Sparus aurata): Molecular cloning, expression and modelling of the complexes with MHC class I

British Library Electronic Table of Contents (United Kingdom)

The T cell receptor is a fundamental mediator of the adaptive immune responses, since TR ab on T cells recognize foreign structures (peptides derived from processed antigens) bound to the major histocompatibility complex (MHC) on APC cells. In the present study, we report the cloning of six TRB chains cDNA sequences from gilthead sea bream (Sparus aurata), a fish of high economical impact in South Mediterranean aquaculture. The V-BETA domains have the canonical features of known teleost and mammalian TR V-BETA domains and have been divided in four different subgroups. A multiple alignment of the six sea bream TRB chains with other known TRB sequences was assembled and showed the conservation of the four cysteine residues involved in disulphide bonds and of some amino acids with an importan...

2008-01-01

420

Stellar Pollution in the Solar Neighborhood

Species in the genus Turritopsis (Cnidaria, Hydrozoa): a molecular evaluation:

We study spectroscopically determined iron abundances of 642 solar-type stars to search for the signature of accreted iron-rich material. We find that the metallicity [Fe/H] of a subset of 466 main sequence stars, when plotted as a function of stellar mass, mimics the pattern seen in lithium abundances in open clusters. Using Monte Carlo models we find that, on average, these stars have accreted about 0.4 Earth masses of iron while on the main sequence. A much smaller sample of 19 stars in the Hertzsprung gap, which are slightly evolved and whose convection zones are significantly more massive, have lower average [Fe/H], and their metallicity shows no clear variation with stellar mass. These findings suggest that terrestrial-type material is common around solar type stars.

2000-01-01

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421

British Library Electronic Table of Contents (United Kingdom)

Abstract Mitochondrial ribosomal gene sequences were used to investigate the status of several populations of hydromedusae belonging to the genus Turritopsis (family Oceaniidae). Several nominal species have been described for this genus, but most of them had been synonymized and attributed to one cosmopolitan species, Turritopsis nutricula. A recent revision based on morphological and reproductive characters, however, has shown that many different populations can be distinguished and that several of the nominal Turritopsis species are likely valid biological species. Our investigation using molecular sequence data of 16S mitochondrial gene confirms these results. The Mediterranean Turritopsis must be attributed to Turritopsis dohrnii and the Turritopsis of New Zealand must be referred to ...

2007-01-01

422

Secure Direct Communication Based on Non-Orthogonal Entangled Pairs and Local Measurement

Resource-loaded planning for ALICE

We propose a quantum secure direct communication scheme based on non-orthogonal entangled pairs and local measurement. In this scheme, we use eight non-orthogonal entangled pairs to act as quantum channels. Due to the non-orthogonality of the quantum channels, the present protocol can availably prohibit from all kinds of valid eavesdropping and acquire a secure quantum channel. By local measurement, the sender acquires a secret random sequence. The process of encoding on the random sequence is identical to the one in one-time-pad. So the present protocol is secure. Even for a highly lossy channel, our scheme is also valid. The scheme is feasible with present-day techniques.

2008-12-01

423

On the disrupted magnetic braking model for the period gap of cataclysmic variables

The ALICE experimental area management team faces various challenges when it comes to sharing scarce resources, indispensable to any efficient installation in a category one worksite. Space, cranes, jigs, and personnel with key competences have to be carefully allocated to activities so as to avoid slowing down work progress. To this intent, a resource loaded planning has been developed that allows highlighting coactivities and prioritizing critical tasks. It uses the built-in capabilities of Microsoft Project. The use of this scheduling tool leads to a more efficient use of time and a safer work environment. The installation sequence resulting from this schedule is presented in this paper. The first part of the sequence focuses on the revision of the coils in the SX2 building. The dipole has then to be installed in the RB26 side of the UX25 cavern. This complex and resource intensive activity has to be performed in parallel with the services ...

2005-01-01

424

New pulse sequence for simultaneous T1, T2, and RHO determination in whole-body proton MR imaging

The disrupted magnetic braking theory for the period gap of cataclysmic variable systems is used to study the binary evolution of low-mass main-sequence-like stars with white dwarf companions. The model is able to reproduce the observed location and width of the gap provided that the average mass transfer rates above the upper edge of the gap are greater than about 1.9 x 10 to the -9th solar masses/yr. For the case of angular momentum loss by magnetic braking, the slope of the mass transfer rate with respect to orbital period is shown to range from 3.4 to 3.7. For the evolutionary sequences considered, the He-3 abundance at the surface of the secondary exceeds 0.0015 after the complete mixing phase, resulting in modifications in the nuclear burning development of nova explosions. 31 refs.

425

Molecular prospecting for cryptic species in Phyllodistomum lacustri (Platyhelminthes, Gorgoderidae)

A newly developed method to support tissue characterization in routine MR imaging is presented. It represents a compromise between the practical requirements of clinical routine (short measurement time, multisection imaging) and the necessary accuracy of the relaxation parameter evaluation. The sequence employed is a modification of the PHAPS technique and works with two excitation pulses. It allows the simultaneous acquisition of all measurement data for the subsequent T1, T2, and rho evaluations. T1 is determined with a specially designed computation formula based on four signal ratios. A multiexponential T2 analysis is performed with use of 28 echoes. From these parameters, relative proton density can be computed.

426

British Library Electronic Table of Contents (United Kingdom)

Rosas-Valdez, R., Choudhury, A. & Prez-Ponce de Len, G. (2011). Molecular prospecting for cryptic species in Phyllodistomum lacustri (Platyhelminthes, Gorgoderidae). -Zoologica Scripta, 40, 296-305. Partial sequences of the 28S ribosomal RNA and cytochrome c oxidase I (COI) genes were compared among populations of a widely distributed and morphologically uniform digenean species in North America, Phyllodistomum lacustri, a parasite characteristically associated with ictalurid catfishes. Specimens were collected from the urinary bladder of ictalurid hosts in six localities of North America, spanning most of the latitudinal range of this freshwater fish group. Sequences of other congeneric species, including a potentially close relative, P.-staffordi, were also obtained and used for comparis...

2011-01-01

427

Molecular characterization of Fasciola spp. from the endemic area of northern Iran based on nuclear ribosomal DNA sequences

British Library Electronic Table of Contents (United Kingdom)

Fasciolosis caused by Fasciola spp. (Platyhelminthes: Trematoda: Digenea) is considered as the most important helminth infection of ruminants in tropical countries, causing considerable socioeconomic problems. In the endemic regions of the North of Iran, Fasciola hepatica and Fasciola gigantica have been previously characterized on the basis of morphometric differences, but the use of molecular markers is necessary to distinguish exactly between species and intermediate forms. Samples from buffaloes and goats from different localities of northern Iran were identified morphologically and then genetically characterized by sequences of the first (ITS-1) and second (ITS-2) Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA). Comparison of the ITS of the northern Iranian samples ...

2011-01-01

428

Metallization of large silicon wafers. Quarterly technical report No. 1, August 26--December 31, 1977. [45 references

Mass-loss in 2D zero-age main-sequence stellar models

A proposed metallization system for large area silicon solar cells with shallow junctions is outlined, and its desirable features are discussed. A baseline process sequence for the nickel palladium metallization system (NPMS) is delineated. This baseline process sequence is serving as the starting point from which process variations are being performed. The eventual goal is optimization of the NPMS process and determination of the control ranges for NPMS process variables. Initial studies of palladium displacement and electroless chemical plating solutions used in the baseline NPMS have begun and progress is reported. In support of this work, an annotated bibliography (45 citations) dealing primarily with palladium plating and palladium-silicon contact formation has been prepared (and will be subject to updating in the future reports).

1977-01-01

429

British Library Electronic Table of Contents (United Kingdom)

Abstract A large number of massive stars are known to rotate rapidly, resulting in a significant distortion and variation in surface temperature from the pole to the equator. Radiatively driven mass-loss is temperature-dependent, so rapid rotation produces a variation in the mass-loss and angular momentum loss rates across the surface of the star, which is expected to affect the evolution of rapidly rotating massive stars. In this work, we use zero-age main-sequence (ZAMS) stellar models to investigate the two-dimensional effects of rotation on stellar mass-loss, using two common prescriptions for radiatively driven mass-loss. The associated loss of angular momentum from these models is also considered. Using 2D stellar models, which give the variation in surface parameters as a function o...

2011-01-01

430

Many-particle confinement by constructed disorder and quantum computing

Isolation of full-length putative rat lysophospholipase cDNA using improved methods for mRNA isolation and cDNA cloning

Many-particle confinement (localization) is studied for a 1D system of spinless fermions with nearest-neighbour hopping and interaction, or equivalently, for an anisotropic Heisenberg spin-1/2 chain. This system is frequently used to model quantum computers with perpetually coupled qubits. We construct a bounded sequence of site energies that leads to strong single-particle confinement of all states on individual sites. We show that this sequence also leads to a confinement of all many-particle states in an infinite system for a time that scales as a high power of the reciprocal hopping integral. The confinement is achieved for strong interaction between the particles while keeping the overall bandwidth of site energies comparatively small. The results show the viability of quantum computing with time-independent qubit coupling.

2005-10-01

431

Inhibition of barium sulfate deposition by polycarboxylates of various molecular structures

The authors have cloned a full-length putative rat pancreatic lysophospholipase cDNA by an improved mRNA isolation method and cDNA cloning strategy using (/sup 32/P)-labelled nucleotides. These new methods allow the construction of a cDNA library from the adult rat pancreas in which the majority of recombinant clones contained complete sequences for the corresponding mRNAs. A previously recognized but unidentified long and relatively rare cDNA clone containing the entire sequence from the cap site at the 5' end to the poly(A) tail at the 3' end of the mRNA was isolated by single-step screening of the library. The size, amino acid composition, and the activity of the protein expressed in heterologous cells strongly suggest this mRNA codes for lysophospholipase.

1987-03-24

432

Human cDNA mapping using fluorescence in situ hybridization. Final progress report, April 1, 1994--July 31, 1997

To establish a relationship between the molecular structure of polycarboxylates and their growth-retarding influence on barium sulfate, seeded-suspension-growth experiments were performed at various inhibitor concentrations and pH values. Two types of polycarboxylates with a molecular structure based on their polyacrylic or maleic acid were studied. The molecular structure of these compounds were varied by particle substitution with monomers containing hydroxyl, amide, and sulfonic acid, as well as hydrophobic groups. Hydrophobic groups are detrimental to good inhibitor performance, whereas the introduction of OH, NH {sub 2}, or SO {sub 3} H groups presents opportunities to enhance the inhibitor effectiveness. The sequence in performance of the compounds on barium sulfate was compared with the sequence formerly obtained for calcium sulfate dihydrate.

1990-02-01

433

Human and rat mast cell high-affinity immunoglobulin E receptors: Characterization of putative. alpha. -chain gene products

The ultimate goal of this research is to generate and apply novel technologies to speed completion and integration of the human genome map and sequence with biomedical problems. To do this, techniques were developed and genome-wide resources generated. This includes a genome-wide Mapped and Integrated BAC/PAC Resource that has been used for gene finding, map completion and anchoring, breakpoint definition and sequencing. In the last period of the grant, the Human Mapped BAC/PAC Resource was also applied to determine regions of human variation and to develop a novel paradigm of primate evolution through to humans. Further, in order to more rapidly evaluate animal models of human disease, a BAC Map of the mouse was generated in collaboration with the MTI Genome Center, Dr. Bruce Birren.

1997-12-31

434

Geomorphic and sedimentologic evidence for the separation of Lake Superior from Lake Michigan and Huron

The authors have cloned and determined the entire nucleotide sequence of cDNAs corresponding to the putative {alpha} subunits of the human and rat mast cell high-affinity IgE receptors. Both human and rat cDNAs encode an NH{sub 2}-terminal signal peptide, two immunoglobulin-like extracellular domains (encoded by discrete exons), a hydrophobic transmembrane region, and a positively charged cytoplasmic tail. The human and rat {alpha} subunits share an overall homology with one another and the immunoglobulin gene family, suggesting that they arose from a common ancestral gene and continue to share structural homology with their ligands. In addition, the rat gene is transcribed into at least three distinct forms, each of which yields a somewhat different coding sequence.

1988-03-01

435

British Library Electronic Table of Contents (United Kingdom)

A common break was recognized in four Lake Superior strandplain sequences using geomorphic and sedimentologic characteristics. Strandplains were divided into lakeward and landward sets of beach ridges using aerial photographs and topographic surveys to identify similar surficial features and core data to identify similar subsurface features. Cross-strandplain, elevation-trend changes from a lowering towards the lake in the landward set of beach ridges to a rise or reduction of slope towards the lake in the lakeward set of beach ridges indicates that the break is associated with an outlet change for Lake Superior. Correlation of this break between study sites and age model results for the strandplain sequences suggest that the outlet change occurred sometime after about 2,400 calendar years...

2007-01-01

436

Geology, physical properties, and surface effects at Discus Thrower Site, Yucca Flat, Nevada Test Site

Genotyping of Candida albicans on the basis of polymorphisms of ALT repeats in the repetitive sequence (RPS)

Geologic studies in connection with Project Discus Thrower have furnished detailed stratigraphic and structural information about northwestern Yucca Flat, Nevada Test Site. The Paleozoic rocks consist of a lower carbonate sequence, argillite of the Eleana Formation, and an upper carbonate sequence. The distribution of these rocks suggests that both top and bottom of the Eleana are structural contacts, probably thrusts or reverse faults. The overlying tuff includes several units recognized in the subsurface, such as the Fraction Tuff and tuff of Redrock Valley. Other units recognized include bedded tuff associated with the Grouse Canyon Member of Belted Range Tuff, and the Rainier Mesa and Ammonia Tanks Members of the Timber Mountain Tuff. The Timber Mountain and Grouse Canyon are extensively altered to montmorillonite (a swelling clay), possibly as a result of ponding of alkaline water. The overlying alluvium locally contains at the base a ...

437

British Library Electronic Table of Contents (United Kingdom)

SummaryBackgroundCandida albicans is one of the most important etiologic agents causing superficial and deep fungal infections. For prevention of candidiasis, it is important to develop a rapid system that discriminates C. albicans at the strain level.ObjectiveTo develop a system that can identify C. albicans at the strain level.MethodsGenomic DNAs were purified from 179 clinical isolates of C. albicans, and were used as templates for PCR amplification of 25S rDNA and ALT repeats in repetitive sequences (RPSs). PCR products generated from ALT repeats were digested with EcoRI and/or ClaI in order to study the relationships between restriction profiles and amplification profiles.ResultsOne hundred and seventy nine clinical isolates were grouped into genotypes A (92 isolates), B (38 isolates)...

2006-01-01

438

Feeding ecology of Xenoturbella bocki (phylum Xenoturbellida) revealed by genetic barcoding

British Library Electronic Table of Contents (United Kingdom)

Abstract The benthic marine worm Xenoturbella is frequently contaminated with molluscan DNA, which had earlier caused confusion resulting in a suggested bivalve relationship. In order to find the source of the contaminant, we have used molluscan sequences derived from Xenoturbella and compared them to barcodes obtained from several individuals of the nonmicroscopic molluscs sharing the same environment as Xenoturbella. Using cytochrome oxidase 1, we found the contaminating sequences to be 98% similar to the bivalve Ennucula tenuis. Using the highly variable D1-D2 region of the large ribosomal subunit in Xenoturbella, we found three distinct species of contaminating molluscs, one of which is 99% similar to the bivalve Abra nitida, one of the most abundant bivalves in the Gullmarsfjord where...

2008-01-01

439

Evidence for a 4700-2100 BC palaeoearthquake recorded in a fluvial-archaeological sequence of the Segura River, SE Spain

British Library Electronic Table of Contents (United Kingdom)

The archaeological excavation of a rock shelter (Abrigo del Pozo) in one of the slopes of the Segura River (SE Spain) has revealed a exceptionally preserved sedimentary record spanning from the Paleolithic to the present-day, which includes an anomalous layer of stones (RFB) fallen from the roof. The sedimentary analysis of the stratigraphic sequence exhumed by the excavation indicates that human occupation of the rock shelter was controlled by fluvial environmental evolution. However, the RFB level resulted in a disturbance of human occupation and normal fluvial sedimentation. From the sedimentary and archaeological pieces of evidence, the RFB level has been interpreted as related to a palaeoearthquake responsible for the collapse of the roof and walls of the rock shelter. The palaeoearth...

2011-01-01

440

Evaluation of a commercial MRSA assay when multiple MRSA strains are causing epidemics

British Library Electronic Table of Contents (United Kingdom)

Rapid and reliable diagnostic methods are needed to control methicillin-resistant Staphylococcus aureus (MRSA) transmission. We studied the BD GeneOhm? MRSA Assay which is based on one specific amplification product at the junction of the right extremity sequence of the staphylococcal cassette chromosome mec (SCCmec) and the chromosomal sequence of orfX of S. aureus. The test was applied on 95 clinical isolates in Finland: 83% were positive. The isolates giving negative results represented several pulsed-field gel electrophoresis (PFGE) types and harboured SCCmec types IV, V, VI or were new types with different combinations of ccr genes.

2009-01-01

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441

Effect of Diaporthe RNA virus 1 (DRV1) on growth and pathogenicity of different Diaporthe species

British Library Electronic Table of Contents (United Kingdom)

A 4.1?kbp positive-strand RNA virus known as Diaporthe RNA virus 1 (DRV1) occurs in hypovirulent, non-sporulating isolates of the fungal pathogen Diaporthe perjuncta. A full-length cDNA clone of DRV1 was developed and RNA transcribed from the cDNA clone used to transfect different Diaporthe spp. The transfected species included three D. ambigua isolates and an unidentified Phomopsis asexual state of a Diaporthe sp. Successful transfections were confirmed using RT-PCR. Although the in vitro-transcribed positive sense single-stranded RNA used for transfection included vector sequences at both ends, the genomes of progeny virus from DRV1-transfected isolates were free of the vector sequences. Transfection resulted in morphological changes in these fungal pathogens. However, the presence of DR...

2011-01-01

442

Description of Cretaceous Sedimentary Sequence of the Yaojia Formation Recovered by CCSD-SK-Is Borehole in Songliao Basin: Lithostratigraphy, Sedimentary Facies and Cyclic Stratigraphy

British Library Electronic Table of Contents (United Kingdom)

The Yaojia Formation recovered by CCSD-SK-Is borehole (China Cretaceous Continental Scientific Drilling-SongkeI-the south borehole) is 157.67 m long and 99.96% of cores recovery. The age of the formation corresponds with a range from the Santonian to the early Campanian. The sequences and processes of lithology-lithofacies and cyclic stratigraphy are revealed by a detailed core description. Eleven rock types and three kinds of sedimentary subfacies, including shallow lake, deep lake, and delta front, are recognized from the drilling core. There are eleven sedimentary microfacies including dolostone, argillaceous limestone, shallow lake turbidite, deep lake turbidite, subaqueous mouth bar, distal bar, sheet sandstone, subaqueous distributary bay, slump deposits, shallow lake mudstone, and d...

2009-01-01

443

Description of Cretaceous Sedimentary Sequence of the Quantou Formation Recovered by CCSD-SK-Is Borehole in Songliao Basin: Lithostratigraphy, Sedimentary Facies and Cyclic Stratigraphy

British Library Electronic Table of Contents (United Kingdom)

The Quantou Formation recovered by CCSD-SK-Is borehole (China Cretaceous Continental Scientific Drilling-SongkeI-the south borehole) is 132.07 m long and the recovery of cores is 100%. The sequence and process of lithology-lithofacies and cyclostratigraphy are revealed by a detailed core description. Nine rock types and three kinds of sedimentary facies including meandering river, shallow lake, and delta front are recognized from the drilling core, there are ten sedimentary microfacies, which are point bar, natural levee, crevasse splay, crevasse channel, floodplain, flood lake, distributary mouth bar, interdistributary bay, mudstone of still water, and turbidite. The Quantou Formation represents seventy-six meter-scale cycles (sixth-order cycle), twenty-five fifth-order cycles, eight four...

2009-01-01

444

Description of Cretaceous Sedimentary Sequence of the First Member of the Qingshankou Formation Recovered by CCSD-SK-Is Borehole in Songliao Basin: Lithostratigraphy, Sedimentary Facies, and Cyclic Stratigraphy

British Library Electronic Table of Contents (United Kingdom)

The first member of the Qingshankou Formation recovered by CCSD-SK-Is borehole (China Cretaceous Continental Scientific Drilling-SongkeI-the south borehole) is 81.41 m long, and the recovery of cores is 100%. The age of the member is approximately Turonian. The sequence and process of lithology-lithofacies and cyclic stratigraphy were revealed by a detailed core description. 6 rock types (mudstone, dolomite, marl, limestone, ostracode limestone, and volcanic ash) comprise the sedimentary strata. Deposition occurred in a deep lake, including 6 sedimentary microfacies: mudstone, sandy turbidite, dolomite, marl, limestone, and volcanic ash microfacies. The first member of the Qingshankou Formation represents 81 meter-scale cycles (sixth-order cycle), 25 fifth-order cycles, 6 fourth-order cycl...

2009-01-01

445

DNA barcoding as a tool for species identification in three forensic wildlife cases in South Africa

British Library Electronic Table of Contents (United Kingdom)

Poaching of wildlife animals for subsistence and commercial purposes has lead to population declines in Africa. In forensic cases, a need exists to identify the species of origin of carcasses, meat or blood. In the study presented here, the mitochondrial COI gene was sequenced to determine the species of unknown samples in three suspect South African forensic wildlife cases. In two cases the unknown samples were identified as originating from domestic cattle (Bos taurus) and in the third case the sample was identified as common reedbuck (Redunca arundinum). This is the first report of the COI sequence of common reedbuck. The study highlights the need for accurate wildlife reference material from each country in order to convict wildlife cases.

2011-01-01

446

Complete genome sequences of Brucella melitensis strains M28 and M5-90, with different virulence backgrounds.

Comparative profiling of the transcriptional response to soybean cyst nematode infection of soybean roots by deep sequencing

Brucella melitensis is a Gram-negative coccobacillus bacteria belonging to the Alphaproteobacteria subclass. It is an important zoonotic pathogen that causes brucellosis, a disease affecting sheep, cattle, and sometimes humans. The B. melitensis strain M5-90, a live attenuated vaccine cultured from the B. melitensis virulent strain M28, has been an effective tool to control brucellosis in goats and sheep in China. Here we report the complete genome sequences of B. melitensis M28 and M5-90, strains with different virulence backgrounds, which will serve as a valuable reference for future studies. PMID:21478357

2011-04-08

447

British Library Electronic Table of Contents (United Kingdom)

To gain insight into the changes in the transcriptome of soybean roots during soybean cyst nematode (SCN) infection, we conducted genome-wide gene expression profiling using serial analysis of gene expression (SAGE) combined with Solexa sequencing. More than 3 million tags were generated from the SCN-infected and uninfected roots, and 366941 and 314591 clean UniTags were obtained from SCN-infected and uninfected samples, respectively. In the SCN-infected sample, 48249 UniTags represented 18114 reference genes. In the uninfected control, 46290 UniTags represented 19323 reference genes. Comparison of tag frequencies identified 1405 genes that were expressed at greater levels in SCN-infected roots than in uninfected roots, and 1191 genes that were expressed at lower levels. Quantitative real-...

2011-01-01

448

Breadcrust bombs as indicators of Vulcanian eruption dynamics at Guagua Pichincha volcano, Ecuador

British Library Electronic Table of Contents (United Kingdom)

Vulcanian eruptions are common at many volcanoes around the world. Vulcanian activity occurs as either isolated sequences of eruptions or as precursors to sustained explosive events and is interpreted as clearing of shallow plugs from volcanic conduits. Breadcrust bombs characteristic of Vulcanian eruptions represent samples of different parts of these plugs and preserve information that can be used to infer parameters of pre-eruption magma ascent. The morphology and preserved volatile contents of breadcrust bombs erupted in 1999 from Guagua Pichincha volcano, Ecuador, thus allow us to constrain the physical processes responsible for Vulcanian eruption sequences of this volcano. Morphologically, breadcrust bombs differ in the thickness of glassy surface rinds and in the orientation and den...

2007-01-01

449

Blind Adaptive Subcarrier Combining Technique for MC-CDMA Receiver in Mobile Rayleigh Channel

Amino acid substitution: its use in detection and analysis of genetic variants

A new subcarrier combining technique is proposed for MC -CDMA receiver in mobile Rayleigh fading channel. It exploits the structure formed by repeating spreading sequences of users on different subcarriers to simultaneously suppress multiple access interference (MAI) and provide implicit channel tracking without any knowledge of the channel amplitudes or training sequences. This is achieved by adaptively weighting each subcarrier in each symbol period by employing a simple gradient descent algorithm to meet the constant modulus (CM) criterion with judicious selection of step-size. Improved BER and user capacity performance are shown with similar complexity in order of O(N) compared with conventional maximum ratio combining and equal gain combining techniques even under high channel Doppler rates.

2011-01-01

450

A genomic library-based amplification approach (GL-PCR) for the mapping of multiple IS6110 insertion sites and strain differentiation of Mycobacterium tuberculosis

Techniques of chemical analysis, amino acid sequencing and autoradiography are being used to study the frequency of incorporation of normally noncoded amino acids into hemoglobins and seminal fluid proteins. We are studying, by the sequencing of radiolabeled proteins followed by the recovery of ["3H] isoleucine phenylthiohydantoin by high-performance liquid chromatography, the frequency at which normally noncoded isoleucine is incorporated into hemoglobin because of base-substitution mutations versus translational errors. Irradiation increases the isoleucine content of human hemoglobin and the frequency of substitution of isoleucine for specific amino acids in rabbit hemoglobin. Studies to date indicate that these techniques have been developed sufficiently for initial analysis of the potential of drugs and environmental pollutants to induce base-substitution mutations in mammalian somatic cells.

451

British Library Electronic Table of Contents (United Kingdom)

Evidence suggests that insertion of the IS6110 element is not without consequence to the biology of Mycobacterium tuberculosis complex strains. Thus, mapping of multiple IS6110 insertion sites in the genome of biomedically relevant clinical isolates would result in a better understanding of the role of this mobile element, particularly with regard to transmission, adaptability and virulence. In the present paper, we describe a versatile strategy, referred to as GL-PCR, that amplifies IS6110-flanking sequences based on the construction of a genomic library. M. tuberculosis chromosomal DNA is fully digested with HincII and then ligated into a plasmid vector between T7 and T3 promoter sequences. The ligation reaction product is transformed into Escherichia coli and selective PCR amplification...

2006-01-01

452

Using tree swallows to monitor impacts of aquatic contamination in Great Lakes areas of concern

Three novel mutations responsible for Cockayne syndrome group A

Tree swallows were used to evaluate movement and potential impacts of contaminants from sediments in Newton Creek (diesel range organics: DROs) and Sheboygan River (PCBs), tributaries to Lake Superior and Lake Michigan, respectively. Contaminated sites occurred along the course of each river, while reference sites were located upstream or on a nearby river. Productivity was monitored and eggs, day 1 nestlings, and day 12 nestlings were collected from each nest. Whole body or egg homogenates were analyzed for PCBs or DROs. EROD activity in livers from day 12 nestlings is being determined for both PCB and DRO exposures. In the Newton Creek study, hatching success was similar for DRO and reference sites. DROs were detected in gastrointestinal tracts of 1 nestling from the reference and 1 from the contaminated site. DROs were not detected in any egg samples. In the Sheboygan River study, hatching success rates differed between 1 reference and 1 contaminated river ...

1995-12-31

453

The role of the state oil company in Latin America

Cockayne syndrome (CS) is a rare autosomal recessive disease, which shows diverse clinical symptoms such as photosensitivity, severe mental retardation and developmental defects. CS cells are hypersensitive to killing by ultraviolet (UV)-irradiation and defective in transcription-coupled repair. Two genetic complementation groups in CS (CS-A and CS-B) have been identified. We analyzed mutations of the CSA gene in 5 CS-A patients and identified 3 types of mutations. Four unrelated CS-A patients (CS2OS, CS2AW, Nps2 and CS2SE) had a deletion including exon 4, suggesting that there is a founder effect on the CSA mutation in Japanese CS-A patients. Patient CS2SE was a compound heterozygote for this deletion and an amino acid substitution at the 106th glutamine to proline (Q106P) in the WD-40 repeat motif of the CSA protein, which resulted in a defective nucleotide excision repair. Patient Mps1 had a large deletion in the upstream region including exon 1 of the CSA gene. ...

2003-02-01

454

Study of Water Quality Value in Sub of the Stream Area of Cisadane Rivers Upstream as Reference Area Based on Macro invertebrate Composition

The role of the Latin American state oil companies (LASOCs) in establishing national industries to fuel economic development is discussed. LASOCs are represented internationally in an organization called ARPEL (Asistencia Reciproca Petrolera Estatal Latinoamericana) which is aimed to foster interchange, cooperation, and mutual assistance among its 20 member companies, as well as to promote economic integration of Latin America through its petroleum sector. State oil companies in Latin America date from 1922, when the oldest LASOC was created in Argentina. LASOCs are responsible for ca 80% of petroleum activities in Latin America. As of 1990, Latin American oil reserves, including gas liquids, amounted to ca 122 billion bbl or 12.2% of the world total. Regional oil production averaged 7.4 million bbl/d in 1990. Refining capacity is ca 7.7 million bbl/d, of which 80% is operated directly by LASOCs. Natural gas reserves are 7.3 trillion m"3, ca 6% of the world total, and production ...

455

SAMFT1D: Single-phase and multiphase flow and transport in 1 dimension. Version 2.0, Documentation and user`s guide

Specific structure of biota community condition can used as indicator for the ecosystem stability value or the waters pollution level where the aquatic biota exist. the macro invertebrates as bio indicator organism can used to assess the upper Cisadane Sub River Basin water quality. The grouping and calculation result from family number, Ept presences (sensitive macro invertebrate), Diversity index (H"'), DO value and TOC value showed 5 criteria of water quality as reference site. This criteria is used to determine water quality level from ten branches of rivers in the upper Cisadane Sub River Basin, Cijeruk districts. The biotic index calculation for ASPT (Average Score Per Taxon) and T and T (index Trihadiningrum and Tjindronegoro) is used to asses water quality based on macro invertebrate composition indicator. Both biotic index system support and give the similar evaluation to water quality level from ten branches of river on the upper Cisadane Sub River Basin. The water quality ...

2001-11-01

456

Remedial investigation/feasibility study report for lower Watts Bar Reservoir Operable Unit

This report documents a one-dimensional numerical model, SAMFT1D, developed to simulate single-phase and multiphase fluid flow and solute transport in variably saturated porous media. The formulation of the governing equations and the numerical procedures used in the code for single-phase and multiphase flow and transport are presented. The code is constructed to handle single-phase as well as two or three-phase flow conditions using two integrated sets of computational modules. The fully implicit scheme is used in the code for both single-phase and multiphase flow simulations. Either the Crank-Nicholson scheme or the fully implicit scheme may be used in the transport simulation. The single-phase modules employ the Galerkin and upstream weighted residual finite element techniques to model flow and transport of water (aqueous phase) containing dissolved single-species contaminants concurrently or sequentially, and include the treatment of various boundary conditions ...

1991-09-01

457

Rehabilitation and modernization of the Jansen pumped-storage power plants; Sanierung und Modernisierung der Pumpspeicher-Kraftwerke Jansen

This document is the combined Remedial Investigation and Feasibility Study Report for the Lower Watts Bar Reservoir (LWBR) Operable Unit (OU). The LWBR is located in Roane, Rhea, and Meigs counties, Tennessee, and consists of Watts Bar Reservoir downstream of the Clinch River. This area has received hazardous substances released over a period of 50 years from the U.S. Department of Energy`s Oak Ridge Reservation (ORR), a National Priority List site established under the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA). As required by this law, the ORR and all off-site areas that have received containments, including LWBR, must be investigated to determine the risk to human health and the environment resulting from these releases, the need for any remedial action to reduce these risks, and the remedial actions that are most feasible for implementation in this OU. Contaminants from the ORR are primarily transported to the LWBR via the Clinch River. ...

1994-08-01

458

Recovery of Water from Boiler Flue Gas

The Jansen pumped-storage plants owned by Bayernwerk Wasserkraft AG are located on the river Pfreimd in eastern Bavaria, Germany. The group comprises the three power plants at Reisach, Tanzmuehle and Trausnitz, as well as the Rabenleite upper reservoir and two weirs. After forty years of service, the power plant group has recently been rehabilitated. The rehabilitation and modernization scheme included, inter alia, replacing the surface sealing on the upstream side of the upper reservoir dam, installing a state-of-the-art leakage and stability monitoring system, and renewing the instrumentation and control equipment in the power plants and in the central control room. Finally, the paper describes the operating experience with the new I and C systems. (orig.) [German] Die Pumpspeicherkraftwerke Jansen der Bayernwerk Wasserkraft AG befinden sich an der Pfreimd in Ostbayern. Die Kraftwerksgruppe besteht aus den 3 Kraftwerken Reisach, Tanzmuehle und Trausnitz sowie aus ...

1999-11-01

459

Partitioning of trace metals in the chemical fractions of bed-load sediments of Nahr-Ibrahim river, Lebanon

This project dealt with use of condensing heat exchangers to recover water vapor from flue gas at coal-fired power plants. Pilot-scale heat transfer tests were performed to determine the relationship between flue gas moisture concentration, heat exchanger design and operating conditions, and water vapor condensation rate. The tests also determined the extent to which the condensation processes for water and acid vapors in flue gas can be made to occur separately in different heat transfer sections. The results showed flue gas water vapor condensed in the low temperature region of the heat exchanger system, with water capture efficiencies depending strongly on flue gas moisture content, cooling water inlet temperature, heat exchanger design and flue gas and cooling water flow rates. Sulfuric acid vapor condensed in both the high temperature and low temperature regions of the heat transfer apparatus, while hydrochloric and nitric acid vapors condensed with the water vapor in the low ...

2008-09-30

460

Method and device for the graduated charging of finely-particulate solid substances into an industrial furnace. Verfahren und Vorrichtung zum dosierten Einfuehren feinkoerniger Feststoffe in einen Industrieofen

Full text.Sediments are the ultimate sink of trace elements. The total metal analysis may only give information concerning possible enrichment of metals. The analysis of metal partitioning in the different chemical components of sediments (exchangeable, carbonate, easily reducible, moderately reducible, organic and residual); give a detailed information on the way in which these metals are bound to sediments, their mobilization capacity and their ability to affect water quality under different environmental conditions. The studied river basin is dominated by limestone formation, the enrichment of metals in the carbonate sediment fraction is a high probability. The objective of the study was to determine the percentage of the total metal content (Fe, Mn, Zn, Cu and Pb) in the six chemical fractions of the bed load sediments of Nahr-Ibrahim river during the dry season and verify the role of carbonate for metal sediment deposition. Bed load sediments were sampled at five locations 13Km ...

1999-11-02

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461

Liquid-metal flow in a sharp elbow in a uniform transverse magnetic field

The invention concerns a method for the graduated introduction of finely-particulate and especially pulverously-particulate solid substance, in particular coal dust, from a pressurised proportioning tank containing a supply of solid matter into an industrial furnace with several supply points, in particular a shaft furnace such as a blast furnace or a cupola furnace, the solid matter being conveyed to the individual supply points in a flow of carrier gas heavily charged with solid matter, through a separate transport line for each supply point, the carrier gas being conveyed to the lower end section of the proportioning tank in a current which causes a local loosening in the lower section of the supply of solid matter, with the transport lines opening into the loosened area, whilst the proportioning tank that contains the solid matter is weighed continuously, the actual weight of the proportioning tank being compared with the latter's target weight, the pressure in the ...

1987-09-09

462

Heat transfer augmentation through wall-shape-induced flow destabilization

In the self-cooling blankets of the Tokamak fusion reactor, a liquid metal, namely liquid lithium, is pumped through a system of ducts to transfer heat and capture neutrons. One of the blanket designs proposed in Argonne National Laboratory's Blanket Comparison and Selection study uses a combination of poloidal and toroidal ducts in order to maximize heat transfer while minimizing net pressure drop. In the design, the poloidal and toroidal ducts meet at sharp, abrupt corners. They were modelled as two identical, straight, semi-infinite, thin-walled, rectangular ducts with 45{degree} miters and joined at a 90{degree} angle in the plane of a strong, uniform magnetic field. While in the toroidal containment vessel (i.e. the blanket), the liquid lithium is subjected to a large electromagnetic body force due to the presence of a strong magnetic field. This body force so dominates the flow as to make the inertial and viscous forces negligible everywhere, except in thin boundary or ...

1989-01-01

463

Gas-liquid flows in a microscale fractal-like branching flow network

Experiments on heat transfer augmentation in a rectangular cross-section water channel are reported. The channel geometry is designed to excite normally damped Tollmein-Schlichting modes in order to enhance mixing. In this experiment, a hydrodynamically fully developed flow encounters a test section where one channel boundary is a series of periodic, saw-tooth, transverse grooves. Free shear layers span the groove openings, separating the main channel flow from the circulating vortices contained within each cavity. The periodicity length of the grooves is equal to one-half of the expected wavelength of the most unstable mode. The remaining channel walls are flat, and the channel has an aspect ratio of 10:1. Experiments are performed over the Reynolds number range of 300 to 15,000. Streakline flow visualization shows that the flow is steady at the entrance, but becomes oscillatory downstream of an onset location. This location moves upstream with increasing Reynolds ...

1990-05-01

464

Gas-liquid flows in a microscale fractal-like branching flow network

Two-phase air-water flows in a microscale fractal-like flow network were experimentally studied and results were compared to predictions from existing macroscale void fraction correlations and flow regime maps. Void fraction was assessed using (1) two-dimensional analysis of high-speed images (direct method) and (2) experimentally determined using gas velocities (indirect method). Fixed downstream-to-upstream length and width ratios of 1.4 and 0.71, respectively, characterize the five-level flow network. Channels were fabricated in a 38 mm diameter silicon disk, 250 {mu}m deep disk with a terminal channel width of 100 {mu}m. A Pyrex top allowed for flow visualization. Superficial air and water velocities through the various branch levels were varied from 0.007 m/s to 1.8 m/s and from 0.05 m/s to 0.42 m/s, respectively. Two-phase flow regime maps were generated for each level of the flow network and are well predicted by the Taitel and Dukler model. Void fraction ...

2009-10-15

465

Flow simulation of the Component Development Integration Facility magnetohydrodynamic power train system

Two-phase air-water flows in a microscale fractal-like flow network were experimentally studied and results were compared to predictions from existing macroscale void fraction correlations and flow regime maps. Void fraction was assessed using (1) two-dimensional analysis of high-speed images (direct method) and (2) experimentally determined using gas velocities (indirect method). Fixed downstream-to-upstream length and width ratios of 1.4 and 0.71, respectively, characterize the five-level flow network. Channels were fabricated in a 38 mm diameter silicon disk, 250 ?m deep disk with a terminal channel width of 100 ?m. A Pyrex top allowed for flow visualization. Superficial air and water velocities through the various branch levels were varied from 0.007 m/s to 1.8 m/s and from 0.05 m/s to 0.42 m/s, respectively. Two-phase flow regime maps were generated for each level of the flow network and are well predicted by the Taitel and Dukler model. Void fraction assessed ...

2009-10-01

466

Experimental validation of large eddy simulations of flow and heat transfer in a stationary ribbed duct

This report covers application of Argonne National Laboratory`s (ANL`s) computer codes to simulation and analysis of components of the magnetohydrodynamic (MHD) power train system at the Component Development and Integration Facility (CDIF). Major components of the system include a 50-MWt coal-fired, two-stage combustor and an MHD channel. The combustor, designed and built by TRW, includes a deswirl section between the first and the second-stage combustor and a converging nozzle following the second-stage combustor, which connects to the MHD channel. ANL used computer codes to simulate and analyze flow characteristics in various components of the MHD system. The first-stage swirl combustor was deemed a mature technology and, therefore, was not included in the computer simulation. Several versions of the ICOMFLO computer code were used for the deswirl section and second-stage combustor. The MGMHD code, upgraded with a slag current leakage submodel, was used for the MHD channel. Whenever ...

1997-11-01

467

Effects of coal syngas and H{sub 2}S on the performance of solid oxide fuel cells. Part 2. Stack tests

Accurate prediction of ribbed duct flow and heat transfer is of importance to the gas turbine industry. The present study comprehensively validates the use of large eddy simulations (LES) for predicting flow and heat transfer with measured flowfield data in a stationary duct with 90"o ribs and elucidates on the detailed physics encountered in the developing flow region, the fully developed region, and the 180"o bend region. Among the major flow features predicted with accuracy are flow transition at the entrance of the duct; the distribution of mean and turbulent quantities in the developing, fully developed, and 180"o bend; the development of secondary flows in the duct cross-section and the 180"o bend; and friction and heat transfer augmentation. At the duct inlet, both the computations and experiments show that the peak turbulence intensities reach values as high as 40% in the streamwise and spanwise directions and 32% in the vertical direction, and a comparison of values along the ...

2006-04-01

468

Effects of amifostine on radiation-induced apoptosis in mouse ovary

The performance of two-cell planar solid oxide fuel cell stacks using coal syngas, with and without hydrogen sulfide (H{sub 2}S), was studied. All cells were tested at 850 C with a constant current load of 15.2 A (current density of 0.22 A cm{sup -2} per cell) and 30% fuel utilization. The H{sub 2}S injection immediately and significantly affected the power degradation of the stack system regardless of the carrier fuel. Results for the test with only H{sub 2} and N{sub 2} in the presence of H{sub 2}S (119-120 ppm) indicated that the power decay and area-specific resistance (ASR) degradation values were lower than those for the tests where simulated syngas containing CO and increased water content was used. The results indicate that contact points in the stack contributed to the power degradation of the system. Other factors, including contamination from the upstream fuel gas tubing, may have contributed to the higher degradation under simulated syngas conditions. ...

2007-02-10

469

ERK-dependent and -independent pathways trigger human neural progenitor cell migration

The present study was designed to assess the radioprotective effects of amifostine on ovarian follicles. Three week-old female mice with or without pretreatment of amifostine were irradiated with 6.42 Gy of #gamma# -ray. Ovaries were collected 0 and 6h after irradiation. DNA fragmentation pattern and expression of genes and activity of proteins related with apoptosis were investigated by means of RT-PCR and Western blot. Proliferation of granulosa cells was reduced and incidence rate of follicular atresia was increased in ovarian follicles in #gamma# -ray irradiated mice compared to those in control or amifostine-treated group. DNA fragmentation was increased in time-dependent manner in granulosa cells of all irradiated groups. However, no difference between amifostine pre-treated group and irradiated groups was found and the expression of p53 as tumor suppressor gene and Bax as one of pro-apoptotic Bcl-2 family was increased in irradiated mice ovaries. PARP as DNA damage sensor was ...

2002-10-20

470

Development requirements for absorption processes for effective CO{sub 2} capture from power plants

Besides differentiation and apoptosis, cell migration is a basic process in brain development in which neural cells migrate several centimeters within the developing brain before reaching their proper positions and forming the right connections. For identifying signaling events that control neural migration and are therefore potential targets of chemicals to disturb normal brain development, we developed a human neurosphere-based migration assay based on normal human neural progenitor (NHNP) cells, in which the distance is measured that cells wander over time. Applying this assay, we investigated the role of the extracellular signal-regulated kinases 1 and 2 (ERK1/2) in the regulation of NHNP cell migration. Exposure to model substances like ethanol or phorbol 12-myristate 13-acetate (PMA) revealed a correlation between ERK1/2 activation and cell migration. The participation of phospho-(P-) ERK1/2 was confirmed by exposure of the cells to the MEK inhibitor PD98059, which directly ...

2007-05-15

471

Development of the Decontamination Approach for the West Valley Demonstration Project Decontamination Project Plan

Effective commercial liquid absorption technology currently applicable for CO{sub 2} capture from power plant flue gases uses a conventional chemical solvent, MEA(monoethanolamine), which was developed over 60 years ago as general non selective solvent to remove acid gases from natural gas streams. The application to CO{sub 2} capture from almost atmospheric pressure flue gases required modification of the technology on account of the oxygen content to incorporate inhibitors to resist solvent degradation and equipment corrosion, as well as upstream treatment of the flue gases to remove both SO{sub x} and NO{sub x} present. The solvent strength used by such processes is relatively low in comparison with high strength modern chemical solvents. The resultant effect is large equipment and high regeneration energy requirements which is a parasitic load on the power plant. Using current technology for CO{sub 2} capture from flue gas derived from conventional pulverized ...

1997-12-31

472

Asymptotic rate of decay of turbulence in a tube following a combustion-induced step in temperature

This paper details the development of a decontamination approach for the West Valley Demonstration Project (WVDP), Decontamination Project Plan (Plan). The WVDP is operated by West Valley Nuclear Services Company (WVNSCO), a subsidiary of Westinghouse Government and Environmental Services, and its parent companies Washington Group International and British Nuclear Fuels Limited (BNFL). The WVDP is a waste management effort being conducted by the United States Department of Energy (DOE) at the site of the only commercial nuclear fuel reprocessing facility to have operated in the United States. This facility is part of the Western New York Nuclear Service Center (WNYNSC), which is owned by the New York State Energy Research and Development Authority (NYSERDA). As authorized by Congress in 1980 through the West Valley Demonstration Project Act (WVDP Act, Public Law 96-368), the DOE's primary mission at the WVDP is to solidify high-level liquid nuclear waste safely; transport the ...

2002-02-25

473

Aquatic macroinvertebrates and water quality of Sandia Canyon, Los Alamos National Laboratory, December 1992--October 1993. Status report

Combustion in a ceramic tube produces a nearly discontinuous change in temperature of the premixed fuel and air at the flame front, from room temperature up to the adiabatic flame temperature ([approximately]2,100 K). The upstream Reynolds number for a stable flame in a 9.5-mm tube is in the range of 3,000-6,000, corresponding to turbulent flow. Owing to property changes that accompany the severe increase in temperature at the flame front, the downstream Reynolds number is reduced below the transitional value ([approximately]2,100); consequently the turbulence decays while the velocity profile approaches the parabolic one characteristic of laminar flow. A previous study of ours revealed that, far downstream from the flame front, the turbulent energy decayed exponentially with downstream distance. This paper examines the asymptotic behavior of the k-[epsilon] model and compares the results to that for two-dimensional (axisymmetric) disturbances in a laminar flow. ...

1993-07-01

474

A phenomenological model of the thermal hydraulics of convective boiling during the quenching of hot rod bundles

In the summer of 1990, an accidental spill from the TA-3 Power Plant Environment Tank released more than 3,785 liters of sulfuric acid into upper Sandia Canyon. The Biological Resource Evaluation Team (BRET) of EM-8 at Los Alamos National Laboratory (LANL) has collected aquatic samples from the stream within Sandia Canyon since then. These field studies gather water quality measurements and collect macroinvertebrates from permanent sampling sites. An earlier report by Bennett (1994) discusses previous BRET aquatic studies in Sandia Canyon. This report updates and expands Bennett's initial findings. During 1993, BRET collected water quality data and aquatic macroinvertebrates at five permanent stations within the canyon. The substrates of the upper three stations are largely sands and silts while the substrates of the two lower stations are largely rock and cobbles. The two upstream stations are located near outfalls that discharge industrial and sanitary waste ...

2004-06-01

475

UniPrime2: a web service providing easier Universal Primer design

In this paper, a phenomenological model of the thermal hydraulics of convective boiling in the post-critical-heat-flux (post-CHF) regime is developed and discussed. The model was implemented in the TRAC-PF1/MOD2 computer code (an advanced best-estimate computer program written for the analysis of pressurized water reactor systems). The model was built around the determination of flow regimes downstream of the quench front. The regimes were determined from the flow-regime map suggested by Ishii and his coworkers. Heat transfer in the transition boiling region was formulated as a position-dependent model. The propagation of the CHF point was strongly dependent on the length of the transition boiling region. Wall-to-fluid film boiling heat transfer was considered to consist of two components: first, a wall-to-vapor convective heat-transfer portion and, second, a wall-to-liquid heat transfer representing near-wall effects. Each contribution was considered separately in each of the inverted ...

1983-10-14

476

The human U1-70K snRNP protein: cDNA cloning, chromosomal localization, expression, alternative splicing and RNA-binding.

The UniPrime2 web server is a publicly available online resource which automatically designs large sets of universal primers when given a gene reference ID or Fasta sequence input by a user. UniPrime2...Full Text Available

2009-07-01

477

The characteristic and relevant fault analysis for the microgovernor synchronization of Daya Bay Nuclear Power Station and Ling'ao Nuclear Power Station

We have isolated and sequenced cDNA clones encoding the human U1-70K snRNP protein, and have mapped this locus (U1AP1) to human chromosome 19. The gene produces two size classes of RNA, a major 1.7-kb...Full Text Available

1987-12-23

478

The YTH Domain Is a Novel RNA Binding Domain*

In Daya Bay and Ling'ao Nuclear Power Station, the Microgovernor has the control functions such as synchronization, block loading etc. The control principle of the Microgovernor is right described by sequence diagram for the first time. That the fault occurred in the synchronization process of Daya Bay Nuclear Power Station unit 1 is also analyzed and illustrated. (author)

2005-09-01

479

Supersonic flutter of composite sandwich panels

The YTH (YT521-B homology) domain was identified by sequence comparison and is found in 174 different proteins expressed in eukaryotes. It is characterized by 14 invariant residues within an α-helix/β-sheet...Full Text Available

2010-05-07

480

Sequence analysis of two alleles reveals that intra-and intergenic recombination played a role in the evolution of the radish fertility restorer (Rfo)

A flutter-motion equation is presently derived for a 2D composite sandwich panel considering the total lateral displacement of the plate as the sum of the displacement due to bending of the plate, and that which is due to shear deformation at the core. The effects of core thickness and stacking sequence of the faces on the flutter boundary of the plate are discussed; it is shown that the sandwich panel greatly improves the flutter boundary over that of a composite laminate panel, provided it has sufficient core thickness.

1992-12-01

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481

Quox-1, a quail homeobox gene expressed in the embryonic central nervous system, including the forebrain.

BackgroundLand plant genomes contain multiple members of a eukaryote-specific gene family encoding proteins with pentatricopeptide repeat (PPR) motifs. Some PPR proteins were shown...Full Text Available

482

Polyadenylated H3 histone transcripts and H3 histone variants in alfalfa.

This paper reports the cloning and sequencing of a quail homeobox-containing gene, Quox-1, and its expression pattern in embryos from 3 to 6 days (E3 to E6) of development as determined by in situ hybridization....Full Text Available

1991-03-15

483

Environmental Research Database

Histone H3 mRNAs were found in polyA(+) fractions of total RNA prepared from alfalfa plants, calli and somatic embryos. The sequence analysis of cDNAs revealed the presence of a polyA tail on independent...Full Text Available

1989-04-25

484

Plant Sulphur Nutrition

DescriptionThe recent sequencing of the complete genomes of several streptomycete species revealed the presence of a large number of cryptic' secondary metabolic gene clusters, and led to the realisation that these organisms have the ability to produce many more natural products than had previously been recognised. One of the aims of our work is to identify the physiological signals and regulatory mechanisms responsible for the activation of these 'cryptic' pathways, thus unleashing the full biosynthetic p [continued...

2010-01-31

485

Perlinhibin, a Cysteine-, Histidine-, and Arginine-Rich Miniprotein from Abalone (Haliotis laevigata) Nacre, Inhibits In Vitro Calcium Carbonate Crystallization

Numerical calculation of the transient response of transmission systems using the natural spline functions, with applications on high-voltage transmission lines

We have isolated a 4.785 Da protein from the nacreous layer of the sea snail Haliotis laevigata (greenlip abalone) shell after demineralization with acetic acid. The sequence of 41...Full Text Available

2007-08-15

486

Multistage skin tumor promotion: involvement of a protein kinase

To determine the transient response of linear and time-invariant transmission systems which are only described for discret frequencies, it is possible to find a good functional approximation by means of the generalized impulse method, combined with the most important natural spline-interpolation functions of the first and the third degree. This procedure has been applied to calculate the step response of the transient behaviour of the zero-sequence impedance system of the high voltage transmission line.

1981-10-01

487

Measurement of the Self-Diffusion Coefficient of Water as a Function of Position in Wheat Grain Using Nuclear Magnetic Resonance Imaging

Current information suggests that chemical carcinogenesis is a multistep process with one of the best studied models in this regard being the two-stage carcinogenesis system using mouse skin. The effects of several carcinogens and tumor promoters in various sequences of application were studied to examine the nature of the process. The actions of several tumor inhibitors were compared. (ACR)

1980-01-01

488

Laser photochemical etching of molybdenum and tungsten thin films by surface halogenation

A pulsed field gradient spin echo sequence has been incorporated in a nuclear magnetic resonance (NMR) imaging experiment to provide an image contrast dependent on local molecular self-diffusion. The...Full Text Available

1988-01-01

489

Human thrombomodulin gene is intron depleted: nucleic acid sequences of the cDNA and gene predict protein structure and suggest sites of regulatory control.

Laser direct-write etching of the refractory metals Mo and W was developed using reactions in chlorine and nitrogen trifluoride vapors. Rate and high spatial resolution are simultaneously optimized using a two-vapor halogenation/development sequence, based on surface modification. Local-area laser chlorination of the metal surface is used to predispose areas to subsequent bulk etching.

1986-12-01

490

Haplotyping and copy number estimation of the highly polymorphic human beta-defensin locus on 8p23 by 454 amplicon sequencing

We have isolated a human thrombomodulin cDNA, and a human genomic clone containing the putative promoter domain, as well as the translated and untranslated regions of the endothelial cell receptor....Full Text Available

1987-09-01

491

Fusion of GFP to the M.EcoKI DNA methyltransferase produces a new probe of Type I DNA restriction and modification enzymes

BackgroundThe beta-defensin gene cluster (DEFB) at chromosome 8p23.1 is one of the most copy number (CN) variable regions of the human genome. Whereas individual DEFB CNs have been...Full Text Available

492

Field guide to the binary stars

AbstractWe describe the fusion of enhanced green fluorescent protein to the C-terminus of the HsdS DNA sequence-specificity subunit of the Type I DNA modification methyltransferase M.EcoKI....Full Text Available

2010-07-23

493

FastBit -- Helps Finding the Proverbial Needle in a Haystack

For most of the history of binary star astronomy, systems have been classified largely on the basis of how they were discovered and qualitative appearance of their spectra and light curves. Present understanding of single and double star evolution has now progressed to the point where most of the classes previously identified, and some new ones, can be arranged into evolutionary sequences, depending primarily on the initial masses and separation of the component stars.

1983-05-12

494

Determination of the isomeric composition and structure of bicyclo(3. 3. 1)nonadienes by PMR spectroscopy

FastBit is a software package designed to meet the searching and filtering needs of data intensive sciences. In these applications, scientists are trying to find nuggets of information from petabytes of raw data. FastBit has been demonstrated to be an order of magnitude faster than comparable technologies. In this brief report, we highlight how we work with a visualization team, a network security team and a DNA sequencing center to find the nuggets in their data.

2006-07-05

495

Characterization of chicken octamer-binding proteins demonstrates that POU domain-containing homeobox transcription factors have been highly conserved during vertebrate evolution

The isomeric composition of bicyclo(3.3.1)nonadienes was investigated by GLC and PMR spectroscopy with silver-lanthanide shift reagents. The absence of stereospecificity in the hydrogenation of 3,7-dimethylenebicyclo(3.3.1)nonane by hydrogen adsorbed on Raney nickel was demonstrated. This is explained by the sequence of the isomerization and then hydrogenation processes.

1986-01-10

496

Characterization and engineering of sequences controlling in vivo synthesis of brome mosaic virus subgenomic RNA.

The DNA sequence motif ATTTGCAT (octamer) or its inverse complement has been identified as an evolutionarily conserved element in the promoter region of immunoglobulin genes. Two major DNA-binding proteins that bind in a sequence-specific manner to the octamer DNA sequence have been identified in mammalian species--a ubiquitously expressed protein (Oct-1) and a lymphoid-specific protein (Oct-2). During characterization of the promoter region of the chicken immunoglobulin light chain gene, the authors identified two homologous octamer-binding proteins in chicken B cells. when the cloning of the human gene for Oct-2 revealed it to be a member of a distinct family of homeobox genes, they sought to determine if the human Oct-2 cDNA could be used to identify homologous chicken homeobox genes. Using a human Oct-2 homeobox-specific DNA probe, they were able to identify 6-10 homeobox-containing genes in the chicken genome, ...

1990-02-01

497

Calculation of fission product behaviour in a station blackout accident of Daya Bay Nuclear Power Plant

Expression of brome mosaic virus (BMV) coat protein and internal genes of many other positive-strand RNA viruses requires initiation of subgenomic mRNA synthesis from specific internal sites on minus-strand...Full Text Available

1988-07-01

498

Amino acid sequences that determine the nuclear localization of yeast histone 2B.

The early accident Sequence of the Station Blackout accident is simulated for Daya Bay Nuclear Power Plant, using MELCOR code. The radioactivity of main fission products was derived after calculating the source term in containment. The data will be used for Daya Bay NPP PSA analysis

2002-12-01

499