WorldWideScience

Sample records for 8-metoxipso raleno mas

  1. Implication of the E. coli K12 uvrA and recA genes in the repair of 8-methoxypsoralen-induced mono adducts and crosslinks on plasmid DNA; Implicacion de los genes uvrA de E. coli K12 en la reparacion de monoaductos y entrecruzamien tos inducidos en DNA plasmidico por 8-metoxipso raleno mas luz ultravioleta A

    Paramio, J.M.; Bauluz, C.; Vidania, R. de

    1986-07-01

    Genotoxicity of psoralen damages on plasmid DNA has been studied. pBR322 DNA was randomly modified with several concentrations of 8-methoxypsoralen plus 365 nm-UV light. After transformation into E. coli strains (wild-type, uvrA and recA) plasmid survival and mutagenesis were analyzed. To study the influence of the SOS response on plasmid recovery, preirradiation of the cells was performed. In absence of cell preirradiation, crosslinks were not repaired in any strain. Mono adducts were also lethal but in part removed by the excision-repair pathway. Preirradiation of the cells significantly. increased plasmid recovery in recA+ celia. In uvrA- only the mutagenic pathway seemed to be involved in the repair of the damaged DNA. Wild type strain showed the highest increase in plasmid survival, involving the repair of mono adducts and some fraction of crosslinks mainly through an error-free repair pathway. This suggests an enhancement of the excision repair promoted by the induction of SOS functions. (Author) 32 refs.

  2. E/Z MAS demonstration

    Los Alamos National Laboratory has developed E/Z MAS, a new generation nuclear material accountability application based on the latest technology and designed for facilities required to track nuclear materials with a simple-to-use interface. E/Z MAS is based on years of experience spent developing nuclear material accounting systems. E/Z MAS uses a modern relational database with a web server and enables users on a classified local area network to interact with the database with web browsers. The E/Z MAS Demonstration poster session demonstrates the E/Z MAS functions required by an operational nuclear facility to track material as it enters and leaves a facility and to account for the material as it moves through a process. The generation of internal facility reports and external reports for the Russian Federal system will be demonstrated. Bar-code readers will be used to demonstrate the ability of EZ MAS to automate certain functions, such as physical inventories at facilities

  3. UAV Robust Strategy Control Based on MAS

    Jian Han; Changhong Wang; Guoxing Yi

    2014-01-01

    A novel multiagent system (MAS) has been proposed to integrate individual UAV (unmanned aerial vehicle) to form a UAV team which can accomplish complex missions with better efficiency and effect. The MAS based UAV team control is more able to conquer dynamic situations and enhance the performance of any single UAV. In this paper, the MAS proposed and established combines the reacting and thinking abilities to be an initiative and autonomous hybrid system which can solve missions involving coo...

  4. Fluid flow dynamics in MAS systems.

    Wilhelm, Dirk; Purea, Armin; Engelke, Frank

    2015-08-01

    The turbine system and the radial bearing of a high performance magic angle spinning (MAS) probe with 1.3mm-rotor diameter has been analyzed for spinning rates up to 67kHz. We focused mainly on the fluid flow properties of the MAS system. Therefore, computational fluid dynamics (CFD) simulations and fluid measurements of the turbine and the radial bearings have been performed. CFD simulation and measurement results of the 1.3mm-MAS rotor system show relatively low efficiency (about 25%) compared to standard turbo machines outside the realm of MAS. However, in particular, MAS turbines are mainly optimized for speed and stability instead of efficiency. We have compared MAS systems for rotor diameter of 1.3-7mm converted to dimensionless values with classical turbomachinery systems showing that the operation parameters (rotor diameter, inlet mass flow, spinning rate) are in the favorable range. This dimensionless analysis also supports radial turbines for low speed MAS probes and diagonal turbines for high speed MAS probes. Consequently, a change from Pelton type MAS turbines to diagonal turbines might be worth considering for high speed applications. CFD simulations of the radial bearings have been compared with basic theoretical values proposing considerably smaller frictional loss values. The discrepancies might be due to the simple linear flow profile employed for the theoretical model. Frictional losses generated inside the radial bearings result in undesired heat-up of the rotor. The rotor surface temperature distribution computed by CFD simulations show a large temperature gradient over the rotor. PMID:26073599

  5. A social-democracia do MAS boliviano

    Guilherme Simões Reis

    2013-01-01

    O partido MAS, que governa a Bolívia e é liderado pelo presidente Evo Morales, geralmente é classificado como populista ou como revolucionário. Este artigo contesta ambos os diagnósticos, e sustenta que o MAS é um partido social-democrata. Tanto em sua gênese, como em seu comportamento na oposição, como em suas políticas no governo, o MAS apresenta todas as características necessárias para ser classificado como um representante da social-democracia. Para contestar os diagnósticos predominante...

  6. Ultra-low temperature MAS-DNP

    Lee, Daniel; Bouleau, Eric; Saint-Bonnet, Pierre; Hediger, Sabine; De Paëpe, Gaël

    2016-03-01

    Since the infancy of NMR spectroscopy, sensitivity and resolution have been the limiting factors of the technique. Regular essential developments on this front have led to the widely applicable, versatile, and powerful spectroscopy that we know today. However, the Holy Grail of ultimate sensitivity and resolution is not yet reached, and technical improvements are still ongoing. Hence, high-field dynamic nuclear polarization (DNP) making use of high-frequency, high-power microwave irradiation of electron spins has become very promising in combination with magic angle sample spinning (MAS) solid-state NMR experiments. This is because it leads to a transfer of the much larger polarization of these electron spins under suitable irradiation to surrounding nuclei, greatly increasing NMR sensitivity. Currently, this boom in MAS-DNP is mainly performed at minimum sample temperatures of about 100 K, using cold nitrogen gas to pneumatically spin and cool the sample. This Perspective deals with the desire to improve further the sensitivity and resolution by providing "ultra"-low temperatures for MAS-DNP, using cryogenic helium gas. Different designs on how this technological challenge has been overcome are described. It is shown that stable and fast spinning can be attained for sample temperatures down to 30 K using a large cryostat developed in our laboratory. Using this cryostat to cool a closed-loop of helium gas brings the additional advantage of sample spinning frequencies that can greatly surpass those achievable with nitrogen gas, due to the differing fluidic properties of these two gases. It is shown that using ultra-low temperatures for MAS-DNP results in substantial experimental sensitivity enhancements and according time-savings. Access to this temperature range is demonstrated to be both viable and highly pertinent.

  7. Increased vascular sympathetic modulation in mice with Mas receptor deficiency

    Rabello Casali, K.; Ravizzoni Dartora, D.; Moura, M; Bertagnolli, M.; Bader, M; Haibara, A.; Alenina, N; Irigoyen, M.C.; Santos, R. A

    2016-01-01

    Introduction: The angiotensin-converting enzyme 2 (ACE2)/angiotensin (Ang)-(1–7)/Mas axis could modulate the heart rate (HR) and blood pressure variabilities (BPV) which are important predictors of cardiovascular risk and provide information about the autonomic modulation of the cardiovascular system. Therefore we investigated the effect of Mas deficiency on autonomic modulation in wild type and Mas-knockout (KO) mice. Methods: Blood pressure was recorded at high sample rate (4000 Hz). Statio...

  8. Nanosatellites for Earth Environmental Monitoring: The MicroMAS Project

    Blackwell, William; Allen, G; Conrad, S.; Galbraith, C.; Kingsbury, R.; Leslie, R; McKinley, P.; Osaretin, I.; W. Osborn; Reid, B.; Retherford, L.; Scarito, M.; Semisch, C.; Shield, M; Silver, M

    2012-01-01

    The Micro-sized Microwave Atmospheric Satellite (MicroMAS) is a dual-spinning 3U CubeSat equipped with a passive microwave spectrometer that observes nine channels near the 118.75-GHz oxygen absorption line. The focus of this MicroMAS mission (hereafter, MicroMAS-1) is to observe convective thunderstorms, tropical cyclones, and hurricanes from a near-equatorial orbit. The MicroMAS-1 flight unit is currently being developed by MIT Lincoln Laboratory, the MIT Space Systems Laboratory, and the M...

  9. A Construction Way of MAS Based on Organization Theory

    GAO Bo; FEI Qi; CHEN Xue-guang

    2002-01-01

    With emphasizing that the integration of autonomy and coordination is the basis for constructing multi-agent systems (MAS), we analyze the organizational characters inherent with MAS and point out that it's a natural and essential way to construct MAS based on organization theory. We consider that the emphasis of the theory is the process of system analyzing. Then we present an analysis frame to expound the process, which includes the process of organization definition, the process of role definition, the process of organizational structure definition and the process of interaction protocol definition. Lastly, we discuss some issues associated with the processes of system design and implementation.

  10. Evaluating Interaction of MAS Providing Context-Aware Services

    Sánchez-Pi, Nayat; Griol, David; Carbó, Javier; José M. Molina

    2011-01-01

    Much effort has been spent on suggesting and implementing new architectures of MAS to specific domains. Often each new architecture is not even compared to any existing architectures in order to evaluate their potential benefits. The evaluation of Multi-Agent Systems (MAS) is a complex problem and it does not have a single form. The present work follows the research line of considering the agent interaction as the main evaluation criteria, the most important characteristic of any complex soft...

  11. An MAS-Based ETL Approach for Complex Data

    Boussaïd, Omar; Darmont, Jérôme

    2008-01-01

    In a data warehousing process, the phase of data integration is crucial. Many methods for data integration have been published in the literature. However, with the development of the Internet, the availability of various types of data (images, texts, sounds, videos, databases...) has increased, and structuring such data is a difficult task. We name these data, which may be structured or unstructured, "complex data". In this paper, we propose a new approach for complex data integration, based on a Multi-Agent System (MAS), in association to a data warehousing approach. Our objective is to take advantage of the MAS to perform the integration phase for complex data. We indeed consider the different tasks of the data integration process as services offered by agents. To validate this approach, we have actually developed an MAS for complex data integration.

  12. 31P Solid-state MAS NMR spectra

    The structures of the silicoaluminiophosphates MCM-1 and MCM9 were characterized by 27Al and 31P MAS NMR. The structural identity of MCM-1 and its silicon-free homologue AlPO4-H3 is demonstrated. The presence of a structural mixture in MCM-9 is confirmed. 31P MAS NMR spectra of MCM-9 could be interpreted as a superposition of spectra of VPI-5, AlPO4-H3 and SAPO-11 phases. (author). 12 refs.; 3 figs.; 1 tab

  13. Structural biology applications of solid state MAS DNP NMR

    Akbey, Ümit; Oschkinat, Hartmut

    2016-08-01

    Dynamic Nuclear Polarization (DNP) has long been an aim for increasing sensitivity of nuclear magnetic resonance (NMR) spectroscopy, delivering spectra in shorter experiment times or of smaller sample amounts. In recent years, it has been applied in magic angle spinning (MAS) solid-state NMR to a large range of samples, including biological macromolecules and functional materials. New research directions in structural biology can be envisaged by DNP, facilitating investigations on very large complexes or very heterogeneous samples. Here we present a summary of state of the art DNP MAS NMR spectroscopy and its applications to structural biology, discussing the technical challenges and factors affecting DNP performance.

  14. Project MAS 1984-1985. OEA Evaluation Report.

    New York City Board of Education, Brooklyn. Office of Educational Assessment.

    This multi-site instructional program (Project MAS) provides instruction in English as a second language and native language arts, as well as bilingual instruction in mathematics and science to approximately 400 Spanish-speaking schools. It serves third through eighth graders at four sites in the Bronx. Its instructional and non-instructional…

  15. 1H MAS, 13C CP/MAS, and 2H NMR spectra studies of piperidinium p-chlorobenzoate

    Anomalous H/D isotope effects were detected in the 1H MAS NMR spectra of piperidinium p-chlorobenzoate (C5H10NH 2+⋅ ClC6H4COO − ) upon deuterium substitution of hydrogen atoms which form two kinds of N-H⋯O H-bonds in the crystal; in contrast to these spectra, only slight chemical shifts were recorded in 13C CP/MAS NMR spectra. 2H NMR spectrum of the deuterated sample show quadrupole coupling constants of 148 and 108 kHz, and reveal that there are a few motions contributing to the electric-field modulation of the 2H nucleus. The 1H MAS NMR spectra of piperidinium p-chlrobenzoate-d16 (C5D10ND 2+⋅ ClC6D4COO − ) and -d14 (C5D10NH 2+⋅ ClC6D4COO − ) revealed that the change in the envelope is caused by chemical shifts of each signal upon deuteration. Calculations based on the density-functional-theory showed that the N-H distance along the crystallographic a-axis mainly contributes to the anomalous isotope effects on 1H MAS NMR envelopes.

  16. DynamicWorkflow in Grid-MAS Integration Context

    Salle, Paola; Duvert, Frédéric; Hérin, Danièle; Stefano A. CERRI

    2007-01-01

    This paper addresses the architectural foundations of dynamic workflows in distributed multi-agent systems (MAS) integrated in Grid context. The purpose is to design an architecture at the same time taking into consideration tasks dependencies among agents, adaptation with respect to historic lessons learnt from past behaviour (memory) and the autonomous decisions when an unpredicted event occurs. In order to do this, given one ontology, called AGIO, which describes Agent-Grid Integration, we...

  17. Commercial facility site selection simulating based on MAS

    Chao, Yi; Li, Qingquan; Zheng, Guizhou

    2008-10-01

    The location of commercial facility decides the benefit of the operator to a large degree. Existing location methods can express the static relationships between site selection result and location factors, but there still are some limites when express the dynamic and uncertain relationship between them. Hence, a dynamic, stochastic and forecastable location model should be built which can introduce the customer's behavior into the model and combine the macro pattern and micro spatial interaction. So the authors proposes Geosim-LM based on MAS. Geosim-LM has 3 kinds of agents, CustAgent, SiteAgent and GovAgent. They represent the customers, commercial fercilities and government. The land type, land price and traffic are the model environment. Then Geosim-LM is applied in the bank branches site evaluation and selection in Liwan district, Guangzhou. In existing bank branches site evaluation, there are 70% consistent in score grade between result of Geosim-LM after 200 round runing and actual rebust location. It proves the model is reliable and feasible. The conclusions can be get from the paper. MAS have advantages in location choice than existed methods. The result of Geosim-LM running can powerfully proves that building location model based on MAS is feasible.

  18. MAS及其相关概念%MAS and Correlation Conceptions

    程显毅; 董红斌

    2000-01-01

    The researching of MAS originates from distributed artificial intelligence ,because new theory framework is provided for solving some problems in complex and distributed environment ,MAS is valued increasingly by computer ,automatic control and management science.

  19. A General Protocol for Temperature Calibration of MAS NMR Probes at Arbitrary Spinning Speeds

    Guan, Xudong; Stark, Ruth E.

    2010-01-01

    A protocol using 207Pb NMR of solid lead nitrate was developed to determine the temperature of magic-angle spinning (MAS) NMR probes over a range of nominal set temperatures and spinning speeds. Using BioMAS and fastMAS probes with typical sample spinning rates of 8 and 35 kHz, respectively, empirical equations were devised to predict the respective sample temperatures. These procedures provide a straightforward recipe for temperature calibration of any MAS probe.

  20. Formalization of the Abstract Architecture of MAS Based on FIPA Specification

    Zheng Liwei(郑丽伟); Yu Xueli; Feng Xiufang

    2003-01-01

    The FIPA specification of MAS (multi-agent system)is accepted by most of the applications of MAS in the world, and has been used in many projects. This paper draws an abstract architecture from the FIPA based MAS, and gives formalization about it.

  1. MAS-SCM for Auto Sector-The Framework

    Ritu Sindhu

    2011-07-01

    Full Text Available The purpose of this study is to describe in detail the development status of the innovative Multi -Agent based supply chain management (MAS-SCMAS for auto sector. This study was undertaken in view of the significance of improvement in efficiency of automobile sector and the development of a good multi-agent system framework to achieve that. The factors affecting the efficiency or OEE of the industry and the efficiency properties were identified. It was clearly revealed that the available model do not fulfill the needs in the supply chain management of automobile industry and there is a scope for evolving new model resulting in better efficiency.

  2. The MAS Six Years in Power in Bolivia

    2012-01-01

    Review Essay of:– The Rise of Evo Morales and the MAS, by Sven Harten. London/New York: Zed Books, 2011.– Evo Morales and the Movimiento al Socialismo in Bolivia; The First Term in Context, edited by Adrian J. Pearce. London, Institute for the Study of the Americas, 2011.– El estado de derecho como tiranía, by Luis Tapia. La Paz: CIDES/UMSA, 2011.– From Rebellion to Reform in Bolivia: Class Struggle, Indigenous Liberation and the Politics of Evo Morales, by Jeffery R. Webber. Chicago: Haymark...

  3. Optimization of MAS and MODIS Polar ocean cloud mask

    Memmen, Sean P.

    2000-01-01

    With the reduction of funding for sea ice reconnaissance flights, the National/Naval Ice Center needs to capitalize on the improvements in satellite technology. Imaging sensors such as AVHRR, DMSP/OLS, SSM/I and RADARSAT have been used to detect the presence of sea ice, but with the exception of SSM/I and RADARSAT, clouds are a major obstacle to viewing the surface. With NASA's development of the Moderate-resolution Imaging Spectroradiometer (MODIS) and MODIS Airborne Simulator (MAS), there i...

  4. Milli-Arcsecond (MAS) Imaging of the Solar Corona

    Davila, Joseph M.; Oktem, Figen S.; Kamalabadi, Farzad; O'Neill, John; Novo-Gradac, Anne-Marie; Daw, Adrian N.; Rabin, Douglas M.

    2016-05-01

    Dissipation in the solar corona is believed to occur in extremely thin current sheets of order 1-100 km. Emission from these hot but thin current sheets should be visible in coronal EUV emission lines. However, this spatial scale is far below the resolution of existing imaging instruments, so these dissipation sites have never been observed individually. Conventional optics cannot be manufactured with sufficient surface figure accuracy to obtain the required spatial resolution in the extreme-ultraviolet where these hot plasmas radiate. A photon sieve, a diffractive imaging element similar to a Fresnel zone plate, can be manufactured to provide a few milli-arcsec (MAS) resolution, with much more readily achievable tolerances than with conventional imaging technology. Prototype photon sieve elements have been fabricated and tested in the laboratory. A full-scale ultra-high resolution instrument will require formation flying and computational image deconvolution. Significant progress has been made in overcoming these challenges, and some recent results in these areas are discussed. A simple design for a sounding rocket concept demonstration payload is presented that obtains 80 MAS (0.080 arcsec) imaging with a 100 mm diameter photon sieve to image Fe XIV 334 and Fe XVI 335. These images will show the structure of the corona at a resolution never before obtained, and they will also allow a study of the temperature structure in the dissipation region.

  5. The MAS Six Years in Power in Bolivia

    Ton Salman

    2012-04-01

    Full Text Available Review Essay of:– The Rise of Evo Morales and the MAS, by Sven Harten. London/New York: Zed Books, 2011.– Evo Morales and the Movimiento al Socialismo in Bolivia; The First Term in Context, edited by Adrian J. Pearce. London, Institute for the Study of the Americas, 2011.– El estado de derecho como tiranía, by Luis Tapia. La Paz: CIDES/UMSA, 2011.– From Rebellion to Reform in Bolivia: Class Struggle, Indigenous Liberation and the Politics of Evo Morales, by Jeffery R. Webber. Chicago: Haymarket Books, 2011.– La democracia desde los márgenes: Transformaciones en el campo político boliviano, by María Teresa Zegada, with Claudia Arce, Gabriela Canedo and Alber Quispe. La Paz: Muela del Diablo Editores/CLACSO, 2011.

  6. MODIS Airborne simulator (MAS) Final Report for CLASIC

    Thomas Arnold; Steven Platnick

    2010-11-24

    The MAS was flown aboard the NASA ER-2 for the CLASIC field experiment, and for all data collected, provided calibrated and geolocated (Level-1B) radiance data for it’s 50 spectral bands (ranging in wavelength for 0.47 to 14.3 µm). From the Level-1B data, as directed in the Statement of Work, higher order (Level-2) data products were derived. The Level-2 products include: a) cloud optical thickness, b) cloud effective radius, c) cloud top height (temperature), d) cloud fraction, e) cloud phase products. Preliminary Level-1B and Level-2 products were provided during the field experiment (typically within one or two days of data collection). Final version data products were made available in December 2008 following considerable calibration analysis. Data collection, data processing (to Level-2), and discussion of the calibration work are summarized below.

  7. Food Waste Composting Study from Makanan Ringan Mas

    Kadir, A. A.; Ismail, S. N. M.; Jamaludin, S. N.

    2016-07-01

    The poor management of municipal solid waste in Malaysia has worsened over the years especially on food waste. Food waste represents almost 60% of the total municipal solid waste disposed in the landfill. Composting is one of low cost alternative method to dispose the food waste. This study is conducted to compost the food waste generation in Makanan Ringan Mas, which is a medium scale industry in Parit Kuari Darat due to the lack knowledge and exposure of food waste recycling practice. The aim of this study is to identify the physical and chemical parameters of composting food waste from Makanan Ringan Mas. The physical parameters were tested for temperature and pH value and the chemical parameter are Nitrogen, Phosphorus and Potassium. In this study, backyard composting was conducted with 6 reactors. Tapioca peel was used as fermentation liquid and soil and coconut grated were used as the fermentation bed. Backyard composting was conducted with six reactors. The overall results from the study showed that the temperature of the reactors were within the range which are from 30° to 50°C. The result of this study revealed that all the reactors which contain processed food waste tend to produce pH value within the range of 5 to 6 which can be categorized as slightly acidic. Meanwhile, the reactors which contained raw food waste tend to produce pH value within the range of 7 to 8 which can be categorized as neutral. The highest NPK obtained is from Reactor B that process only raw food waste. The average value of Nitrogen is 48540 mg/L, Phosphorus is 410 mg/L and Potassium is 1550 mg/L. From the comparison with common chemical fertilizer, it shows that NPK value from the composting are much lower than NPK of the common chemical fertilizer. However, comparison with NPK of organic fertilizer shown only slightly difference value in NPK.

  8. Perencanaan Disain Deep Dig Arm pada Kapal Water Witch Untuk Pengerukan Sampah di Kali Mas Surabaya

    Tony Bambang Musriyadi; Erno Setyawan; Irfan Syarif Arief

    2014-01-01

    Kali Mas merupaka salah satu Sungai di Surabaya yang bermanfaat bagi hajat hidup penduduk Surabaya,Namun seiring berjalan waktu Kali Mas mengalami pendangkalan dan polusi sampah padat akibat pembuangan sampah sembarangan.Oleh karena itu penting untuk melakuakan pengerukan sendimen dan sampah yang efektif dan tepat agar tidak menggangu fungsi utama dari Kali Mas,maka perlu suatu alat untuk pengerukan,Backhoe dreger merupak alat yang tepat untuk pengerukan, akan tetapi harus disesuaikan lengan ...

  9. An HR-MAS MR Metabolomics Study on Breast Tissues Obtained with Core Needle Biopsy

    MuLan Li; Yonghyun Song; Nariya Cho; Jung Min Chang; Hye Ryoung Koo; Ann Yi; Hyeonjin Kim; Sunghyouk Park; Woo Kyung Moon

    2011-01-01

    BACKGROUND: Much research has been devoted to the development of new breast cancer diagnostic measures, including those involving high-resolution magic angle spinning (HR-MAS) magnetic resonance (MR) spectroscopic techniques. Previous HR-MAS MR results have been obtained from post-surgery samples, which limits their direct clinical applicability. METHODOLOGY/PRINCIPAL FINDINGS: In the present study, we performed HR-MAS MR spectroscopic studies on 31 breast tissue samples (13 cancer and 18 non...

  10. MOS-2: A Two-Dimension Space for Positioning MAS Organizational Models

    Abbas, Hosny; Shaheen, Samir

    2015-01-01

    The increased complexity and dynamism of present and future Multi-Agent Systems (MAS) enforce the need for considering both of their static (design-time) and the dynamic (run-time) aspects. A type of balance between the two aspects can definitely give better results related to system stability and adaptivity. MAS organization is the research area that is concerned with these issues and it is currently a very active and interesting research area. Designing a MAS with an initial organization an...

  11. The utility of MAS5 expression summary and detection call algorithms

    Wilson Claire L

    2007-07-01

    Full Text Available Abstract Background Used alone, the MAS5.0 algorithm for generating expression summaries has been criticized for high False Positive rates resulting from exaggerated variance at low intensities. Results Here we show, with replicated cell line data, that, when used alongside detection calls, MAS5 can be both selective and sensitive. A set of differentially expressed transcripts were identified that were found to be changing by MAS5, but unchanging by RMA and GCRMA. Subsequent analysis by real time PCR confirmed these changes. In addition, with the Latin square datasets often used to assess expression summary algorithms, filtered MAS5.0 was found to have performance approaching that of its peers. Conclusion When used alongside detection calls, MAS5 is a sensitive and selective algorithm for identifying differentially expressed genes.

  12. Sealed rotors for in situ high temperature high pressure MAS NMR

    Hu, Jian Z. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Hu, Mary Y. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Zhao, Zhenchao [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Xu, Souchang [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Vjunov, Aleksei [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Shi, Hui [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Camaioni, Donald M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Peden, Charles H. F. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Lercher, Johannes A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2015-07-06

    Magic angle spinning (MAS) nuclear magnetic resonance (NMR) investigations on heterogeneous samples containing solids, semi-solids, liquid and gases or a mixture of them under non-conventional conditions of a combined high pressure and high temperature, or cold temperature suffer from the unavailability of a perfectly sealed rotor. Here, we report the design of reusable and perfectly-sealed all-zircornia MAS rotors. The rotors are easy to use and are suitable for operation temperatures from below 0 to 250 °C and pressures up to 100 bar. As an example of potential applications we performed in situ MAS NMR investigations of AlPO₄-5 molecular sieve crystallization, a kinetic study of the cyclohexanol dehydration reaction using 13C MAS NMR, and an investigation of the metabolomics of intact biological tissue at low temperature using 1H HR-MAS NMR spectroscopy. The in situ MAS NMR experiments performed using the reported rotors allowed reproduction of the results from traditional batch reactions, while offering more detailed quantitative information at the molecular level, as demonstrated for the molecular sieve synthesis and activation energy measurements for cyclohexanol dehydration. The perfectly sealed rotor also shows promising application for metabolomics studies using 1H HR-MAS NMR.

  13. Mas-Colell, Whinston and Green Versus Scitovsky on Profit and Utility Maximization

    Ormazabal Sánchez, Kepa Mirena

    2005-01-01

    I contrast the theoretical foundation of profit maximization of Mas-Colell, Whinston and Green’s “Microeconomics” against that provided by Scitovsky in a paper of 1943. Whereas Mas-Colell, Whinston and Green try to show that profit maximization can be derived from utility maximization, Scitovsky categorically states the contrary view. I argue, first, that the foundation provided by Mas-Colell, Whinston and Green is not sound and, secondly, that Scitovsky’s line of reasoning opens a better way...

  14. A New Algebraic Modelling Approach to Distributed Problem-Solving in MAS

    帅典勋; 邓志东

    2002-01-01

    This paper is devoted to a new algebraic modelling approach to distributed problem-solving in multi-agent systems (MAS), which is featured by a unified framework for describing and treating social behaviors, social dynamics and social intelligence. A conceptual architecture of algebraic modelling is presented. The algebraic modelling of typical social behaviors, social situation and social dynamics is discussed in the context of distributed problemsolving in MAS. The comparison and simulation on distributed task allocations and resource assignments in MAS show more advantages of the algebraic approach than other conventional methods.

  15. The effect of FF-MAS on porcine cumulus-oocyte complex maturation, fertilization and pronucleus formation in vitro

    Færge, Inger; Strejcek, Frantisek; Laurincik, Jozef;

    2006-01-01

    Follicular fluid meiosis-activating sterol (FF-MAS) has been isolated from the follicular fluid (FF) of several species including man. FF-MAS increases the quality of in vitro oocyte maturation, and thus the developmental potential of oocytes exposed to FF-MAS during in vitro maturaion is improve...

  16. Cassini's Maneuver Automation Software (MAS) Process: How to Successfully Command 200 Navigation Maneuvers

    Yang, Genevie Velarde; Mohr, David; Kirby, Charles E.

    2008-01-01

    To keep Cassini on its complex trajectory, more than 200 orbit trim maneuvers (OTMs) have been planned from July 2004 to July 2010. With only a few days between many of these OTMs, the operations process of planning and executing the necessary commands had to be automated. The resulting Maneuver Automation Software (MAS) process minimizes the workforce required for, and maximizes the efficiency of, the maneuver design and uplink activities. The MAS process is a well-organized and logically constructed interface between Cassini's Navigation (NAV), Spacecraft Operations (SCO), and Ground Software teams. Upon delivery of an orbit determination (OD) from NAV, the MAS process can generate a maneuver design and all related uplink and verification products within 30 minutes. To date, all 112 OTMs executed by the Cassini spacecraft have been successful. MAS was even used to successfully design and execute a maneuver while the spacecraft was in safe mode.

  17. BOREAS Level-1B MAS Imagery At-sensor Radiance, Relative X and Y Coordinates

    Strub, Richard; Strub, Richard; Newcomer, Jeffrey A.; Ungar, Stephen

    2000-01-01

    For BOReal Ecosystem-Atmosphere Study (BOREAS), the MODIS Airborne Simulator (MAS) images, along with the other remotely sensed data, were collected to provide spatially extensive information over the primary study areas. This information includes detailed land cover and biophysical parameter maps such as fraction of Photosynthetically Active Radiation (fPAR) and Leaf Area Index (LAI). Collection of the MAS images occurred over the study areas during the 1994 field campaigns. The level-1b MAS data cover the dates of 21-Jul-1994, 24-Jul-1994, 04-Aug-1994, and 08-Aug-1994. The data are not geographically/geometrically corrected; however, files of relative X and Y coordinates for each image pixel were derived by using the C-130 INS data in a MAS scan model. The data are provided in binary image format files.

  18. DYNAMO-MAS: a multi-agent system for ontology evolution from text

    Sellami, Zied; Camps, Valérie; Aussenac-Gilles, Nathalie

    2013-01-01

    International audience Manual ontology development and evolution are complex and time-consuming tasks, even when textual documents are used as knowledge sources in addition to human expertise or existing ontologies. Processing natural language in text produces huge amounts of linguistic data that need to be filtered out and structured. To support both of these tasks, we have developed DYNAMO-MAS, an interactive tool based on an adaptive multi-agent system (adaptive MAS or AMAS) that builds...

  19. {sup 29}Si MAS NMR for the zeolite Y - gallium oxide system; {sup 29}Si mas NMR dla ukladu fojazyt-tlenek galu

    Sulikowski, B.; Derewinski, M. [Inst. Katalizy i Fizykochemii Powierzchni, Polska Akademia Nauk, Cracow (Poland); Olejniczak, Z.; Segnowski, S. [Institute of Nuclear Physics, Cracow (Poland)

    1994-12-31

    Wide-pore zeolites modified by gallium oxide has been prepared for catalytic use. Its physico-chemical and catalytic properties have been studied. The structure changes of the catalyst have been investigated by means of MAS NMR spectroscopy. Spectra of {sup 29}Si has been described and discussed. 11 refs, 4 figs, 2 tabs.

  20. A software framework for analysing solid-state MAS NMR data

    Solid-state magic-angle-spinning (MAS) NMR of proteins has undergone many rapid methodological developments in recent years, enabling detailed studies of protein structure, function and dynamics. Software development, however, has not kept pace with these advances and data analysis is mostly performed using tools developed for solution NMR which do not directly address solid-state specific issues. Here we present additions to the CcpNmr Analysis software package which enable easier identification of spinning side bands, straightforward analysis of double quantum spectra, automatic consideration of non-uniform labelling schemes, as well as extension of other existing features to the needs of solid-state MAS data. To underpin this, we have updated and extended the CCPN data model and experiment descriptions to include transfer types and nomenclature appropriate for solid-state NMR experiments, as well as a set of experiment prototypes covering the experiments commonly employed by solid-sate MAS protein NMR spectroscopists. This work not only improves solid-state MAS NMR data analysis but provides a platform for anyone who uses the CCPN data model for programming, data transfer, or data archival involving solid-state MAS NMR data.

  1. A software framework for analysing solid-state MAS NMR data.

    Stevens, Tim J; Fogh, Rasmus H; Boucher, Wayne; Higman, Victoria A; Eisenmenger, Frank; Bardiaux, Benjamin; van Rossum, Barth-Jan; Oschkinat, Hartmut; Laue, Ernest D

    2011-12-01

    Solid-state magic-angle-spinning (MAS) NMR of proteins has undergone many rapid methodological developments in recent years, enabling detailed studies of protein structure, function and dynamics. Software development, however, has not kept pace with these advances and data analysis is mostly performed using tools developed for solution NMR which do not directly address solid-state specific issues. Here we present additions to the CcpNmr Analysis software package which enable easier identification of spinning side bands, straightforward analysis of double quantum spectra, automatic consideration of non-uniform labelling schemes, as well as extension of other existing features to the needs of solid-state MAS data. To underpin this, we have updated and extended the CCPN data model and experiment descriptions to include transfer types and nomenclature appropriate for solid-state NMR experiments, as well as a set of experiment prototypes covering the experiments commonly employed by solid-sate MAS protein NMR spectroscopists. This work not only improves solid-state MAS NMR data analysis but provides a platform for anyone who uses the CCPN data model for programming, data transfer, or data archival involving solid-state MAS NMR data. PMID:21953355

  2. A software framework for analysing solid-state MAS NMR data

    Stevens, Tim J.; Fogh, Rasmus H.; Boucher, Wayne [University of Cambridge, Department of Biochemistry (United Kingdom); Higman, Victoria A. [University of Oxford, Department of Biochemistry (United Kingdom); Eisenmenger, Frank; Bardiaux, Benjamin; Rossum, Barth-Jan van; Oschkinat, Hartmut [Leibniz-Institut fuer Molekulare Pharmakologie, Department of Structural Biology (Germany); Laue, Ernest D., E-mail: e.d.laue@bioc.cam.ac.uk [University of Cambridge, Department of Biochemistry (United Kingdom)

    2011-12-15

    Solid-state magic-angle-spinning (MAS) NMR of proteins has undergone many rapid methodological developments in recent years, enabling detailed studies of protein structure, function and dynamics. Software development, however, has not kept pace with these advances and data analysis is mostly performed using tools developed for solution NMR which do not directly address solid-state specific issues. Here we present additions to the CcpNmr Analysis software package which enable easier identification of spinning side bands, straightforward analysis of double quantum spectra, automatic consideration of non-uniform labelling schemes, as well as extension of other existing features to the needs of solid-state MAS data. To underpin this, we have updated and extended the CCPN data model and experiment descriptions to include transfer types and nomenclature appropriate for solid-state NMR experiments, as well as a set of experiment prototypes covering the experiments commonly employed by solid-sate MAS protein NMR spectroscopists. This work not only improves solid-state MAS NMR data analysis but provides a platform for anyone who uses the CCPN data model for programming, data transfer, or data archival involving solid-state MAS NMR data.

  3. The development of the ProMAS: a Probabilistic Medication Adherence Scale

    Kleppe M

    2015-03-01

    Full Text Available Mieke Kleppe,1,2 Joyca Lacroix,2 Jaap Ham,1 Cees Midden1 1Human Technology Interaction, Eindhoven University of Technology, Eindhoven, the Netherlands; 2Behavior, Cognition and Perception, Philips Research, Eindhoven, the Netherlands Abstract: Current self-report medication adherence measures often provide heavily skewed results with limited variance, suggesting that most participants are highly adherent. This contrasts with findings from objective adherence measures. We argue that one of the main limitations of these self-report measures is the limited range covered by the behaviors assessed. That is, the items do not match the adherence behaviors that people perform, resulting in a ceiling effect. In this paper, we present a new self-reported medication adherence scale based on the Rasch model approach (the ProMAS, which covers a wide range of adherence behaviors. The ProMAS was tested with 370 elderly receiving medication for chronic conditions. The results indicated that the ProMAS provided adherence scores with sufficient fit to the Rasch model. Furthermore, the ProMAS covered a wider range of adherence behaviors compared to the widely used Medication Adherence Report Scale (MARS instrument, resulting in more variance and less skewness in adherence scores. We conclude that the ProMAS is more capable of discriminating between people with different adherence rates than the MARS. Keywords: questionnaire design, probabilistic models, methodology

  4. Residue specific hydration of primary cell wall potato pectin identified by solid-state 13C single-pulse MAS and CP/MAS NMR spectroscopy.

    Larsen, Flemming H; Byg, Inge; Damager, Iben; Diaz, Jerome; Engelsen, Søren B; Ulvskov, Peter

    2011-05-01

    Hydration of rhamnogalacturonan-I (RG-I) derived from potato cell wall was analyzed by (13)C single-pulse (SP) magic-angle-spinning (MAS) and (13)C cross-polarization (CP) MAS nuclear magnetic resonance (NMR) and supported by (2)H SP/MAS NMR experiments. The study shows that the arabinan side chains hydrate more readily than the galactan side chains and suggests that the overall hydration properties can be controlled by modifying the ratio of these side chains. Enzymatic modification of native (NA) RG-I provided samples with reduced content of arabinan (sample DA), galactan (sample DG), or both side chains (sample DB). Results of these samples suggested that hydration properties were determined by the length and character of the side chains. NA and DA exhibited similar hydration characteristics, whereas DG and DB were difficult to hydrate because of the less hydrophilic properties of the rhamnose-galacturonic acid (Rha-GalA) backbone in RG-I. Potential food ingredient uses of RG-I by tailoring of its structure are discussed. PMID:21462966

  5. Technology Enhanced Learning for People with Intellectual Disabilities and Cerebral Paralysis: The MAS Platform

    Colomo-Palacios, Ricardo; Paniagua-Martín, Fernando; García-Crespo, Ángel; Ruiz-Mezcua, Belén

    Education for students with disabilities now takes place in a wide range of settings, thus, including a wider range of assistive tools. As a result of this, one of the most interesting application domains of technology enhanced learning is related to the adoption of learning technologies and designs for people with disabilities. Following this unstoppable trend, this paper presents MAS, a software platform aimed to help people with severe intellectual disabilities and cerebral paralysis in their learning processes. MAS, as a technology enhanced learning platform, provides several tools that supports learning and monitoring for people with special needs, including adaptative games, data processing and monitoring tools. Installed in a special needs education institution in Madrid, Spain, MAS provides special educators with a tool that improved students education processes.

  6. Dibujo y pensamiento en la obra de Rafael Masó

    Marañón González, Rafael Carlos

    2002-01-01

    Rafael Masó, arquitecto noucentista, es además, el artista adecuado para el análisis de una obra totalmente gráfica.Dibujos, escritos, gráficos y bocetos, son los elementos idóneos para que las ideas sobre la expresión gráfica, queden reflejados en esta Tesis Doctoral.Para conocer su obra gráfica, es necesario llegar a lo más profundo de sus pensamientos transmitidos por historiadores y la Familia Masó. Tanto su obra arquitectónica, como sus dibujos, son conocidos por expertos y estudiosos ...

  7. 29Si and 27Al MAS NMR spectra of mullites from different kaolinites.

    He, Hongping; Guo, Jiugao; Zhu, Jianxi; Yuan, Peng; Hu, Cheng

    2004-04-01

    Mullites synthesized from four kaolinites with different random defect densities have been studied by 27Al and 29Si magic angle spinning nuclear magnetic resonance spectroscopy (MAS NMR) and X-ray diffraction (XRD). All these mullites show the same XRD pattern. However, 29Si and 27Al MAS NMR spectra reveal that the mullites derived from kaolinites with high defect densities, have a sillimanite-type Al/Si ordering scheme and are low in silica, whereas those mullites derived from kaolinites with low defect densities, consist of both sillimanite- and mullite-type Al/Si ordering schemes and are rich in silica. PMID:15084323

  8. Learning by observing: information spillovers in the execution and valuation of commercial bank M&As

    Gayle DeLong; Robert DeYoung

    2004-01-01

    We hypothesize that banks become better able to manage acquisitions, and investors become better able to value those acquisitions, as these parties ‘learn-by-observing’ information that spills-over from previous bank M&As. We find evidence consistent with these hypotheses for 216 M&As of large, publicly traded U.S. commercial banks between 1987 and 1999. Our theory and our results are predicated on the idea that acquisitions of large and increasingly complex commercial banks were a relatively...

  9. Two-Stage MAS Technique for Analysis of DRA Elements and Arrays on Finite Ground Planes

    Larsen, Niels Vesterdal; Breinbjerg, Olav

    2007-01-01

    A two-stage Method of Auxiliary Sources (MAS) technique is proposed for analysis of dielectric resonator antenna (DRA) elements and arrays on finite ground planes (FGPs). The problem is solved by first analysing the DRA on an infinite ground plane (IGP) and then using this solution to model the FGP...

  10. Sealed rotors for in situ high temperature high pressure MAS NMR.

    Hu, Jian Zhi; Hu, Mary Y; Zhao, Zhenchao; Xu, Suochang; Vjunov, Aleksei; Shi, Hui; Camaioni, Donald M; Peden, Charles H F; Lercher, Johannes A

    2015-09-11

    Here we present the design of reusable and perfectly sealed all-zirconia MAS rotors. The rotors are used to study AlPO4-5 molecular sieve crystallization under hydrothermal conditions, high temperature high pressure cyclohexanol dehydration reaction, and low temperature metabolomics of intact biological tissue. PMID:26171928

  11. Advanced instrumentation for DNP-enhanced MAS NMR for higher magnetic fields and lower temperatures

    Matsuki, Yoh; Idehara, Toshitaka; Fukazawa, Jun; Fujiwara, Toshimichi

    2016-03-01

    Sensitivity enhancement of MAS NMR using dynamic nuclear polarization (DNP) is gaining importance at moderate fields (B0 90 K) with potential applications in chemistry and material sciences. However, considering the ever-increasing size and complexity of the systems to be studied, it is crucial to establish DNP under higher field conditions, where the spectral resolution and the basic NMR sensitivity tend to improve. In this perspective, we overview our recent efforts on hardware developments, specifically targeted on improving DNP MAS NMR at high fields. It includes the development of gyrotrons that enable continuous frequency tuning and rapid frequency modulation for our 395 GHz-600 MHz and 460 GHz-700 MHz DNP NMR spectrometers. The latter 700 MHz system involves two gyrotrons and a quasi-optical transmission system that combines two independent sub-millimeter waves into a single dichromic wave. We also describe two cryogenic MAS NMR probe systems operating, respectively, at T ∼100 K and ∼30 K. The latter system utilizes a novel closed-loop helium recirculation mechanism, achieving cryogenic MAS without consuming any cryogen. These instruments altogether should promote high-field DNP toward more efficient, reliable and affordable technology. Some experimental DNP results obtained with these instruments are presented.

  12. Cp-MAS solid state NMR of secondary metabolites from northeastern Brazil plants

    Aiming to learn more about the 13 C NMR of secondary metabolites in the solid state, as well as to make use of the Cp-MAS probe available in the CENAUREMN (Northeastern Center for Application and Use od NMR) laboratory, an analysis has been performed on the the following six classes of secondary metabolites: diterpenes, coumarins, alkaloids, flavonoids and purines

  13. Acceptance Test Report for the Modular Automation System (MAS) Manufactured by Honeywell Inc

    This document details the performance of the acceptance test of the Honeywell MAS Control System for equipment to be installed in gloveboxes HA-20MB and HA-211 at a later date. Equipment that was anticipated included 6 stabilization furnaces, only three and their associated equipment were installed

  14. Perencanaan Disain Deep Dig Arm pada Kapal Water Witch Untuk Pengerukan Sampah di Kali Mas Surabaya

    Tony Bambang Musriyadi

    2014-03-01

    Full Text Available Kali Mas merupaka salah satu Sungai di Surabaya yang bermanfaat bagi hajat hidup penduduk Surabaya,Namun seiring berjalan waktu Kali Mas mengalami pendangkalan dan polusi sampah padat akibat pembuangan sampah sembarangan.Oleh karena itu penting untuk melakuakan pengerukan sendimen dan sampah yang efektif dan tepat agar tidak menggangu fungsi utama dari Kali Mas,maka perlu suatu alat untuk pengerukan,Backhoe dreger merupak alat yang tepat untuk pengerukan, akan tetapi harus disesuaikan lengan penerukannya agar efektif dalam beroperasi.Dalam pembuatan Tugas Akhir ini akan di disain Boom dan Arm dari Backhoe dreger untuk pengerukan di Kali mas di Surabaya.Sesuai dengan Kebutuhan dan aspek pertimbangan ukuran kapal telah ditentukan disain Boom dengan panjang 4.6m dan arm 2.1m dengan kapasitas Bucket 0.56 m3 .Dengan pemilihan matrial   Carbon Stell Sheet (SS 1023 dan ketebalan plat 10  mm,metode yang digunakan untuk analisa pembebanan adalah stress analisis pada Program Solid work,dan sebagai pertimbangan kelayakan

  15. An HR-MAS MR metabolomics study on breast tissues obtained with core needle biopsy.

    MuLan Li

    Full Text Available BACKGROUND: Much research has been devoted to the development of new breast cancer diagnostic measures, including those involving high-resolution magic angle spinning (HR-MAS magnetic resonance (MR spectroscopic techniques. Previous HR-MAS MR results have been obtained from post-surgery samples, which limits their direct clinical applicability. METHODOLOGY/PRINCIPAL FINDINGS: In the present study, we performed HR-MAS MR spectroscopic studies on 31 breast tissue samples (13 cancer and 18 non-cancer obtained by percutaneous core needle biopsy. We showed that cancer and non-cancer samples can be discriminated very well with Orthogonal Projections to Latent Structure-Discriminant Analysis (OPLS-DA multivariate model on the MR spectra. A subsequent blind test showed 69% sensitivity and 94% specificity in the prediction of the cancer status. A spectral analysis showed that in cancer cells, taurine- and choline-containing compounds are elevated. Our approach, additionally, could predict the progesterone receptor statuses of the cancer patients. CONCLUSIONS/SIGNIFICANCE: HR-MAS MR metabolomics on intact breast tissues obtained by core needle biopsy may have a potential to be used as a complement to the current diagnostic and prognostic measures for breast cancers.

  16. Hyperspectral Microwave Atmospheric Sounder (HyMAS) architecture and design accommodations

    Hilliard, L.; Racette, P.; Blackwell, W.; Galbraith, C.; Thompson, E.

    The Hyperspectral Microwave Atmospheric Sounder (HyMAS) is being developed at Lincoln Laboratories and accommodated by the Goddard Space Flight Center for a flight opportunity on a NASA research aircraft. The term “ hyperspectral microwave” is used to indicate an all-weather sounding that performs equivalent to hyperspectral infrared sounders in clear air with vertical resolution of approximately 1 km. Deploying the HyMAS equipped scanhead with the existing Conical Scanning Microwave Imaging Radiometer (CoSMIR) shortens the path to a flight demonstration. Hyperspectral microwave is achieved through the use of independent RF antennas that sample the volume of the Earth's atmosphere through various levels of frequencies, thereby producing a set of dense, spaced vertical weighting functions. The simulations proposed for HyMAS 118/183-GHz system should yield surface precipitation rate and water path retrievals for small hail, soft hail, or snow pellets, snow, rainwater, etc. with accuracies comparable to those of the Advanced Technology Microwave Sounder. Further improvements in retrieval methodology (for example, polarization exploitation) are expected. The CoSMIR instrument is a packaging concept re-used on HyMAS to ease the integration features of the scanhead. The HyMAS scanhead will include an ultra-compact Intermediate Frequency Processor (IFP) module that is mounted inside the door to improve thermal management. The IFP is fabricated with materials made of Low-Temperature Co-fired Ceramic (LTCC) technology integrated with detectors, amplifiers, A/D conversion and data aggregation. The IFP will put out 52 channels of 16 bit data comprised of 4 - 9 channel data streams for temperature profiles and 2-8 channel streams for water vapor. With the limited volume of the existing CoSMIR scanhead and new HyMAS front end components, the HyMAS team at Goddard began preliminary layout work inside the new drum. Importing and re-using models of the shell, the s- an head

  17. Hyperspectral Microwave Atmospheric Sounder (HyMAS) Architecture and Design Accommodations

    Hilliard, Lawrence; Racette, Paul; Blackwell, William; Galbraith, Christopher; Thompson, Erik

    2013-01-01

    The Hyperspectral Microwave Atmospheric Sounder (HyMAS) is being developed at Lincoln Laboratories and accommodated by the Goddard Space Flight Center for a flight opportunity on a NASA research aircraft. The term "hyperspectral microwave" is used to indicate an all-weather sounding that performs equivalent to hyperspectral infrared sounders in clear air with vertical resolution of approximately 1 km. Deploying the HyMAS equipped scanhead with the existing Conical Scanning Microwave Imaging Radiometer (CoSMIR) shortens the path to a flight demonstration. Hyperspectral microwave is achieved through the use of independent RF antennas that sample the volume of the Earth s atmosphere through various levels of frequencies, thereby producing a set of dense, spaced vertical weighting functions. The simulations proposed for HyMAS 118/183-GHz system should yield surface precipitation rate and water path retrievals for small hail, soft hail, or snow pellets, snow, rainwater, etc. with accuracies comparable to those of the Advanced Technology Microwave Sounder. Further improvements in retrieval methodology (for example, polarization exploitation) are expected. The CoSMIR instrument is a packaging concept re-used on HyMAS to ease the integration features of the scanhead. The HyMAS scanhead will include an ultra-compact Intermediate Frequency Processor (IFP) module that is mounted inside the door to improve thermal management. The IFP is fabricated with materials made of Low-Temperature Co-fired Ceramic (LTCC) technology integrated with detectors, amplifiers, A/D conversion and data aggregation. The IFP will put out 52 channels of 16 bit data comprised of 4-9 channel data streams for temperature profiles and 2-8 channel streams for water vapor. With the limited volume of the existing CoSMIR scanhead and new HyMAS front end components, the HyMAS team at Goddard began preliminary layout work inside the new drum. Importing and re-using models of the shell, the scan head computer

  18. A novel BN–MAS system composite ceramics with greatly improved mechanical properties prepared by low temperature hot-pressing

    A novel composite ceramics with excellent mechanical properties was fabricated by means of low temperature hot-pressing using hexagonal boron nitride (h-BN) and magnesium aluminum silicate (MAS) as raw materials. The influences of starting MAS content on the microstructural evolution and mechanical properties of the composites were investigated. The results indicate that the effective enhancement of relative density of composites has been achieved, which shows that MAS is an effective liquid-phase sintering aid during the hot-pressing. MAS also can improve the structural ordering of h-BN flakes. On the other hand, h-BN exhibits significant inhibiting effect on the crystallization of α-Cordierite. Furthermore, h-BN flakes with layered structure can play a role in strengthening the MAS matrix. So h-BN and MAS are considered to be co-enhanced by each other, resulting in better sintering ability and the mechanical properties of composite ceramics are better than that of both h-BN and MAS. Composite ceramics incorporated with 50 wt% MAS exhibits the highest bending strength and fracture toughness of 213±25 MPa and 2.49±0.35 MPa m1/2, respectively

  19. Comparison of the 1H NMR analysis of solids by the CRAMPS and MAS-only techniques

    Dec, Steven F.; Bronnimann, Charles E.; Wind, Robert A.; Maciel, Gary E.

    1H NMR spectra are reported on eight representative solid samples, including pure powdered crystalline samples, synthetic organic polymers, a silica gel, HY zeolite, and a lignite. Spectra were obtained by the following three approaches: (1) single pulse on a static sample, (2) CRAMPS, and (3) single pulse with magic-angle spinning (MAS-only). The MAS-only results were obtained as a function of MAS speed. Although the MAS-only technique is capable of achieving a significant degree of line narrowing, even with modest MAS speeds, MAS-only spectra of the general quality of the apparently undistorted high-resolution 1H spectra obtained by the CRAMPS technique are not obtained at the highest MAS speeds examined (21 kHz for a polymethylmethacrylate sample), unless the 1H- 1H dipolar interactions in the sample are rather weak, as with silica gel or a zeolite. Thus, caution should be exercised in interpreting 1H MAS-only spectra, especially if CRAMPS results are not available as a calibration.

  20. Angiotensin-(1-7 attenuates disuse skeletal muscle atrophy in mice via its receptor, Mas

    María Gabriela Morales

    2016-04-01

    Full Text Available Immobilization is a form of disuse characterized by a loss of strength and muscle mass. Among the main features are decreased IGF-1/Akt signalling and increased ubiquitin-proteasome pathway signalling, which induce greater myosin heavy chain degradation. Activation of the classical renin-angiotensin system (RAS causes deleterious effects in skeletal muscle, including muscle wasting. In contrast, angiotensin-(1-7 [Ang-(1-7], a peptide of the non-classical RAS, produces beneficial effects in skeletal muscle. However, the role of Ang-(1-7 in skeletal muscle disuse atrophy and independent of classical RAS activation has not been evaluated. Therefore, we assessed the functions of Ang-(1-7 and the Mas receptor in disuse muscle atrophy in vivo using unilateral cast immobilization of the hind limb in male, 12-week-old wild-type (WT and Mas-knockout (Mas KO mice for 1 and 14 days. Additionally, we evaluated the participation of IGF-1/IGFR-1/Akt signalling and ubiquitin-proteasome pathway expression on the effects of Ang-(1-7 immobilization-induced muscle atrophy. Our results found that Ang-(1-7 prevented decreased muscle strength and reduced myofiber diameter, myosin heavy chain levels, and the induction of atrogin-1 and MuRF-1 expressions, all of which normally occur during immobilization. Analyses indicated that Ang-(1-7 increases IGF-1/IGFR-1/Akt pathway signalling through IGFR-1 and Akt phosphorylation, and the concomitant activation of two downstream targets of Akt, p70S6K and FoxO3. These anti-atrophic effects of Ang-(1-7 were not observed in Mas KO mice, indicating crucial participation of the Mas receptor. This report is the first to propose anti-atrophic effects of Ang-(1-7 via the Mas receptor and the participation of the IGF-1/IGFR-1/Akt/p70S6K/FoxO3 mechanism in disuse skeletal muscle atrophy.

  1. Magic-angle-spinning NMR (MAS-NMR) spectroscopy and the structure of zeolites

    After outlining the chemical features and properties which make zeolites such an important group of catalysts and sorbents, the article explains how high-resolution solid-state NMR with magic-angle spinning reveals numerous new insights into their structure. 29Si-MAS-NMR readily and quantitatively identifies five distinct Si(OAl)sub(n)(OSi)sub(4-n) structural groups in zeolitic frameworks (n=0,1,...4), corresponding to the first tetrahedral coordination shell of a silicon atom. Many catalytic and other chemical properties of zeolites are governed by the short-range Si, Al order, the nature of which is greatly clarified by 29Si-MAS-NMR. (orig./EF)

  2. Research on MAS-Based Supply Chain Resilience and Its Self-Organized Criticality

    Liang Geng

    2014-01-01

    Full Text Available Building resilient supply chain is an effective way to deal with uncertain risks. First, by analyzing the self-organization of supply chain, the supply chain resilience is described as a macroscopic property that generates from self-organizing behavior of each enterprise on the microlevel. Second, a MAS-based supply chain resilience model is established and its local fitness function, neighborhood structure, and interaction rules that are applicable to supply chain system are designed through viewing the enterprise as an agent. Finally, with the help of a case, we find that there is an agglomeration effect and a SOC characteristic in supply chain and the evolution of supply chain is controlled by parameters of MAS. Managers can control the supply chain within the resilient range and choose a good balance between interest and risk by controlling enterprises’ behavior.

  3. Bibliotēku sistēmas klientu portāls

    Bērziņš, Edgars

    2016-01-01

    „Bibliotēkas sistēmas klientu portāls” ir tīmeklī bāzēts portāls, kurš nodrošina uzņēmuma „Bibliotēkas Informācijas sistēmas ALISE” klientiem ērtu un drošu informācijas iegūšanu un ir viegli administrējams portāls. Projekts ir izstrādāts pēc spējas (agile) izstrādes metodes. Portāla izstrādē ir izmantota tīmekļa lietojumprogrammu sistēma MVC, kura pamatā izmanto ASP.NET tīmekļa vietņu programmēšanas valodu.

  4. MAINTAINING THE EFFICIENCY OF MAS METHOD IN CEREALS WHILE REDUCING THE COSTS

    Odile Moullet; Arnold Schori

    2014-01-01

    Marker-assisted selection (MAS) is a powerful tool too rarely exploited in practical breeding applications mainly because of its prohibitive costs. A new manual protocol has been developed for DNA extraction and polymerase chain reaction (PCR) analyses which could increase the impact of this technology on the creation of new varieties. In this procedure, only the amount of DNA serving as template is extracted directly into PCR tubes. The method is reproducible (100 %) and efficient (97.9 %). ...

  5. The Neuroprotective Effect of Cornus mas on Brain Tissue of Wistar Rats

    Renata Francik; Jadwiga Kryczyk; Mirosław Krośniak; Mehmet Berköz; Ilona Sanocka; Sławomir Francik

    2014-01-01

    Cornelian cherry (Cornus mas) is a valuable source of phenolic antioxidants. Flavonoid derivatives as nonenzymatic antioxidants are important in the pathophysiology of many diseases including neurological disorders (e.g., Alzheimer’s disease) or heart disease. In this study, we examined the effect of an addition of freeze-dried fruit of cornelian cherry on three types of diets: control diet, fructose diet, and diet enriched in fats (high-fat diet). This effect was studied by determining the f...

  6. TANGGUNG JAWAB EKSPEDISI MUATAN KAPAL LAUT DALAM PENGIRIMAN BARANG MELALUI LAUT DI PELABUHAN TANJUNG MAS SEMARANG

    PANDEIROTH, NOVRY NOLDY

    2015-01-01

    The title of this thesis is the, the responsibility of the expedition cargo marine through the ocean freight preformance in port Tanjung Mas Semarang. The problems of this study are : How is the responsibility of marine Cargo event of damage goods delivered and how the evidence and the process of compensation. This thesis is a type of empirical legal research,this approach is a focus of research conducted on the behavior of the legal community . Empirical legal research conducted through fiel...

  7. PENGEMBANGAN EKOWISATA BERBASIS KERAKYATAN DI BANJAR NYUH KUNING, DESA MAS, UBUD

    AGUNG SRI SULISTYAWATI

    2015-01-01

    The objective of this study is (1) to identify the potential of Banjar Nyuh Kuning Mas Village, Ubud has to offer as means of attraction in ecotourism which may be accomplished by identifying physical and non physical potential. (2) to develope a community based strategy in ecotourism. The sampling technique used is purposive sampling and accidental sampling. Data collection is accomplished through observatioan, questionaire, focus group discussion and documentation. Data analysis is by quali...

  8. Detection of Ethambutol - resistant Mycobacterium tuberculosis strains by MAS-PCR method and comparison with Proportion

    M. Asgharzadeh

    2007-01-01

    Full Text Available Abstract Background and purpose: Ethambutol (EMB is one of the first - line drugs used for anti-tubercular therapy but resistance to this medicine is developed in many parts of the world. EMB resistant strains commonly have embB mutations. Purpose of this research was detection of EMB-resistant Mycobactercium tuberculosis strains isolated from patients by MAS-PCR method and comparison with Proportion procedure.Materials and Methods: One hundred and twenty M. tuberculosis strains were isolated from patients with tulerculosis in Tabriz TB research center. Susceptibility testing to EMB was performed by the Proportion method. DNA was isolated from cultivated cells by SDS-proteinase K modified method. Isolated DNA was used as the template for PCR reaction.Results: One hundred and sixteen strains were susceptible to EMB and 4 (3.33% strains were resistant to EMB. All EMB resistant strains were multidrug-resistant. The MAS-PCR method was used to evaluate of mutation in the embB306 codon. Mutation was seen at the embB306 codon in all resistant strains to ethambutol.Conclusion: The results showed that MAS-PCR method can be used as a simple and rapid procedure for detecting EMB-resistance in Mycobacterium tuberculosis strains.

  9. Modeling Lyman-\\alpha\\ Forest Cross-Correlations with LyMAS

    Lochhaas, Cassandra; Peirani, Sébastien; Dubois, Yohan; Colombi, Stéphane; Blaizot, Jérémy; Font-Ribera, Andreu; Pichon, Christophe; Devriendt, Julien

    2015-01-01

    We use the Ly-$\\alpha$ Mass Association Scheme (LyMAS; Peirani et al. 2014) to predict cross-correlations at z = 2.5 between dark matter halos and transmitted flux in the Ly-$\\alpha$ forest, and we compare these predictions to cross-correlations measured for quasars and damped Ly-$\\alpha$ systems (DLAs) from the Baryon Oscillation Spectroscopic Survey (BOSS) by Font-Ribera et al. (2012, 2013). We calibrate and test LyMAS using Horizon-AGN hydrodynamical cosmological simulations of a $(100\\ h^{-1}\\ \\rm{Mpc})^3$ comoving volume with and without AGN feedback. We apply this calibration to a $(1\\ h^{-1}\\ \\rm{Gpc})^3$ simulation realized with $2048^3$ dark matter particles for our primary predictions. In the $100\\ h^{-1}\\ \\rm{Mpc}$ box, LyMAS reproduces the halo-flux correlations computed from the full hydrodynamic gas distribution essentially perfectly. In the $1\\ h^{-1}\\ \\rm{Gpc}$ box, the amplitude of the cross-correlation tracks the halo bias as expected, and the correlation for a halo sample with a distributio...

  10. A solid state MAS NMR study of the thermal reactions in alkali-leached aluminosilicates

    The thermal transformations of aluminosilicate minerals such as kaolinite (Al2Si2O5(OH)4) are of importance for the production of both clay-based ceramics and high-technology ceramics such as sialons. Solid-state MAS NMR can provide information about the intermediate stages in the formation of mullite (Al6Si2O13). These intermediates, which are only poorly crystalline and less amenable to XRD study, may include poorly crystalline mullite, a cubic spinel similar to γ-Al2O3 but which has been suggested to contain Si, and other amorphous aluminosilicate phases of variable composition. Since the 29Si and 27Al MAS NMR spectra of all these phases are expected to contain resonances broadly in the same spectral area, unambiguous differentiation of these phases has not so far proved possible by this technique. The work reported here was suggested by the possibility of selective alkali extraction of some of the more silica-rich phases using techniques developed by Chakravorty and Ghosh, which was hoped to reveal the MAS NMR features of the less-leachable phases. NMR study of the leached products after subsequent thermal treatment also provided useful information about the leaching reactions themselves. Copyright (1999) Australasian Ceramic Society

  11. NHS-MAS3: a bifunctional chelator alternative to NHS-MAG3

    This laboratory uses an N-hydroxysuccinimide derivative of S-acetylmercaptoacetyltriglycine (NHS-MAG3) to conjugate amines for subsequent labeling with 99mTc. However, the synthesis from triglycerine is general and not restricted to this tripeptide. We had earlier selected a small number of alternative tripeptides and synthesized the corresponding NHS derivatives. Each was then evaluated in a search for bifunctional chelators with properties superior to NHS-MAG3, such as lower serum protein binding or improved stability to cysteine challenge. Based on these preliminary results, NHS-S-acetylmercaptoacetyltriserine (NHS-MAS3) was selected for further investigation. We have now conjugated this bifunctional chelator to biocytin and to an amine-derivatized peptide nucleic acid (PNA). Both carriers were also conjugated with NHS-MAG3 under identical conditions and all were labeled with 99mTc at neutral pH and at boiling temperature while the conjugated PNAs were radiolabelled at neutral pH and at room temperature. Regardless of the chelator, reverse phase HPLC radiochromatograms of the labeled biotins and PNAs after purification showed a single peak. However, by size exclusion HPLC, the radiochromatograms always showed several peaks even after purification, but the MAS3 radiochromatograms were less complicated. For biotin and PNA both, radiolabeling via MAS3 showed improved 99mTc stability in 37 deg. C serum and in cysteine solution. The four preparations were administered to mice implanted in one thigh with avidin beads (biotins) or complementary PNA beads (PNAs). At 5 h post-administration, no significant differences were observed in the targeting of PNA beads between the two chelators, however the target thigh/normal thigh ratio was significantly higher for MAS3-biotin compared to MAG3-biotin. We conclude that labeling biocytin and amine-derivatized PNA with NHS-MAS3 compared to NHS-MAG3 provides simpler radiochromatographic profiles, improved stability of the label in

  12. Exposure (mAs) optimisation of a multi-detector CT protocol for hepatic lesion detection: are thinner slices better?

    The purpose of this work was to determine the exposure-optimised slice thickness for hepatic lesion detection with CT. A phantom containing spheres (diameter 9.5, 4.8 and 2.4mm) with CT density 10 HU below the background (50 HU) was scanned at 125, 100, 75 and 50 mAs. Data were reconstructed at 5-, 3- and 1-mm slice thicknesses. Noise, contrast-to-noise ratio (CNR), area under the curve (AUC) as calculated using receiver operating characteristic analysis and sensitivity representing lesion detection were calculated and compared. Compared with the 125 mAs/5mm slice thickness setting, significant reductions in AUC were found for 75 mAs (P<0.01) and 50 mAs (P<0.05) at 1- and 3-mm thicknesses, respectively; sensitivity for the 9.5-mm sphere was significantly reduced for 75 (P<0.05) and 50 mAs (P<0.01) at 1-mm thickness; sensitivity for the 4.8-mm sphere was significantly lower for 100, 75 and 50 mAs at all three slice thicknesses (P<0.05). The 2.4-mm sphere was rarely detected. At each slice thickness, noise at 100, 75 and 50 mAs exposures was approximately 10, 30 and 50% higher, respectively, than that at 125 mAs exposure. CNRs decreased in an irregular manner with reductions in exposure and slice thickness. This study demonstrated no advantage to using slices below 5mm thickness, and consequently thinner slices are not necessarily better.

  13. Pengaruh Inovasi Dan Jiwa Kewirausahaan Terhadap Keberhasilan Usaha (Studi Kasus: Pelaku UMKM Kuliner Chinatown Asia Mega Mas Medan)

    Mawaddah, Nadia Inda

    2016-01-01

    This research was conducted to examine the effect of innovation and entrepreneurs Spirit to business success (Case Study: SMEs Of Kuliner Chinatown Asia Mega Mas Medan). The research was conducted on SMEs in the Asia Mega Mas with a total sample of 35 businesses. The sampling method used in this research was saturated sampling by using descriptive analysis method, statistical analysis method whis consist of double linear regression analysis, simultaneous significance test (F-test), partial si...

  14. The novel 10-item asthma prediction tool: external validation in the German MAS birth cohort.

    Linus B Grabenhenrich

    Full Text Available A novel non-invasive asthma prediction tool from the Leicester Cohort, UK, forecasts asthma at age 8 years based on 10 predictors assessed in early childhood, including current respiratory symptoms, eczema, and parental history of asthma.We aimed to externally validate the proposed asthma prediction method in a German birth cohort.The MAS-90 study (Multicentre Allergy Study recorded details on allergic diseases prospectively in about yearly follow-up assessments up to age 20 years in a cohort of 1,314 children born 1990. We replicated the scoring method from the Leicester cohort and assessed prediction, performance and discrimination. The primary outcome was defined as the combination of parent-reported wheeze and asthma drugs (both in last 12 months at age 8. Sensitivity analyses assessed model performance for outcomes related to asthma up to age 20 years.For 140 children parents reported current wheeze or cough at age 3 years. Score distribution and frequencies of later asthma resembled the Leicester cohort: 9% vs. 16% (MAS-90 vs. Leicester of children at low risk at 3 years had asthma at 8 years, at medium risk 45% vs. 48%. Performance of the asthma prediction tool in the MAS-90 cohort was similar (Brier score 0.22 vs. 0.23 and discrimination slightly better than in the original cohort (area under the curve, AUC 0.83 vs. 0.78. Prediction and discrimination were robust against changes of inclusion criteria, scoring and outcome definitions. The secondary outcome 'physicians' diagnosed asthma at 20 years' showed the highest discrimination (AUC 0.89.The novel asthma prediction tool from the Leicester cohort, UK, performed well in another population, a German birth cohort, supporting its use and further development as a simple aid to predict asthma risk in clinical settings.

  15. (11)B MAS NMR and First-Principles Study of the [OBO3] Pyramids in Borates.

    Zhou, Bing; Sun, Wei; Zhao, Biao-Chun; Mi, Jin-Xiao; Laskowski, Robert; Terskikh, Victor; Zhang, Xi; Yang, Lingyun; Botis, Sanda M; Sherriff, Barbara L; Pan, Yuanming

    2016-03-01

    Borates are built from the [Bϕ3] planar triangles and the [Bϕ4] tetrahedral groups, where ϕ denotes O or OH. However, the [Bϕ4] groups in some borates are highly distorted to include three normal B-O bonds and one anomalously long B-O bond and, therefore, are best described as the [OBO3] pyramids. Four synthetic borates of the boracite-type structures (Mg3B7O13Br, Cu3B7O13Br, Zn3B7O13Cl, and Mg3B7O13Cl) containing a range of [OBO3] pyramids were investigated by multifield (7.05, 14.1, and 21.1 T) (11)B magic-angle spinning nuclear magnetic resonance (MAS NMR), triple quantum (3Q) MAS NMR experiments, as well as density functional theory calculations. The high-resolution (11)B MAS NMR spectra supported by theoretical predictions show that the [OBO3] pyramids are characterized by isotropic chemical shifts δiso((11)B) from 1.4(1) to 4.9(1) ppm and nuclear quadrupole parameters CQ((11)B) up to 1.3(1) MHz, both significantly different from those of the [BO4] and [BO3] groups in borates. These δiso((11)B) and CQ((11)B) values indicate that the [OBO3] pyramids represent an intermediate state between the [BO4] tetrahedra and [BO3] triangles and demonstrate that the (11)B NMR parameters of four-coordinate boron oxyanions are sensitive to local structural environments. The orientation of the calculated unique electronic field gradient tensor element Vzz of the [OBO3] pyramids is aligned approximately along the direction of the anomalously long B-O bond, corresponding to B-2pz with the lowest electron density. PMID:26914372

  16. Deuterium and lithium-6 MAS NMR studies of manganese oxide electrode materials

    Paik, Younkee

    Electrolytic manganese dioxide (EMD) is used world wide as the cathode materials in both lithium and alkaline primary (non-rechargeable) batteries. We have developed deuterium and lithium MAS NMR techniques to study EMD and related manganese oxides and hydroxides, where diffraction techniques are of limited value due to a highly defective nature of the structures. Deuterons in EMD, manganite, groutite, and deuterium-intercalated pyrolusite and ramsdellite were detected by NMR, for the first time, and their locations and motions in the structures were analyzed by applying variable temperature NMR techniques. Discharge mechanisms of EMD in alkaline (aqueous) electrolytes were studied, in conjunction with step potential electrochemical spectroscopic (SPECS) method, and five distinctive discharge processes were proposed. EMD is usually heat-treated at about 300--400°C to remove water to be used in lithium batteries. Details of the effects of heat-treatment, such as structural and compositional changes as a function of heat-treatment temperature, were studied by a combination of MAS NMR, XRD, and thermogravimetric analysis. Lithium local environments in heat-treated EMD (HEMD) that were discharged in lithium cells, were described in terms of related environments found in model compounds pyrolusite and ramsdellite where specific Li + sites were detected by MAS NMR and the hyperfine shift scale method of Grey et al. Acid-leaching of Li2MnO3 represents an approach for synthesizing new or modified manganese oxide electrode materials for lithium rechargeable batteries. Progressive removal of lithium from specific crystallographic sites, followed by a gradual change of the crystal structure, was monitored by a combination of NMR and XRD techniques.

  17. Design and implementation of a multi-axis precision movement machine based on MAS theory

    Li MA; Linlin CI; Genyan GE

    2009-01-01

    A model construction of a multi-agent system (MAS) and the basic function of the agent are described.The precision control method using the multi-CPU of a programmable logic controller (PLC) is introduced,and a distributed method using multiple CPUs to control different motion machines is given.The test results indicate that in industrial control fields,the combination of using the credible PLC to control the motion machine and multi-CPU task distributing methods can solve multi-axis machine linkage and implication,providing a more credible method for multi-axis motion units.

  18. Characterization of cornelian cherry (Cornus mas L.) genotypes - genetic resources for food production in Czech Republic

    Sochor Jiri; Jurikova Tunde; Ercisli Sezai; Mlcek Jiri; Baron Mojmir; Balla Stefan; Yilmaz Suzan Ozturk; Necas Thomas

    2014-01-01

    The aim of the study was to determine and compare both technological properties and polyphenol content in fruits of eight cornellian cherry (Cornus mas L.) cultivars from Czech Republic. The fruits of cultivar ´Vydubeckij´ had the highest dry matter (17.4%) and soluble solid (15.8%) content among searched cultivars. The highest crude protein and phosphorus values were determined in the fruits of the ´Titus´ cultivar as 10.9 g of crude protein and 435 mg of ...

  19. MAS Equipped with Ant Colony Applied into Dynamic Job Shop Scheduling

    Kang, Kai; Zhang, Ren Feng; Yang, Yan Qing

    This paper presents a methodology adopting the new structure of MAS(multi-agent system) equipped with ACO(ant colony optimization) algorithm for a better schedule in dynamic job shop. In consideration of the dynamic events in the job shop arriving indefinitely schedules are generated based on tasks with ant colony algorithm. Meanwhile, the global objective is taken into account for the best solution in the actual manufacturing environment. The methodology is tested on a simulated job shop to determine the impact with the new structure.

  20. Production and characterization of a bacteriocin-like inhibitory substance produced by indigenous soil associated pseudomonas putida mas-1

    Bacteriocins have been the subject of extensive research globally due to wide range applications. The aim of this research was to investigate the production of bacteriocin(s) or bacteriocin like inhibitory substance(s) by Pseudomonas putida MAS-1 strain. The bacteriocin produced (Putidacin MAS-1) was found bioactive against clinical Staphylococcus aureus and Enterococcus faecalis strains. Bioactivity was observed by stab and overlay assay and multiwell antagonistic activity assay. Putidacin MAS-1 was sensitive beyond 70 degree C but stable at wide pH range (3 to 8). Bioactivity of putidacin MAS-1 was lost after treatment with trypsin and protease while partially lost after Proteinase K treatment. Treatment with ethanol, methanol, chloroform, acetone Tween 20 and Tween 80 showed partial decrease in bioactivity. SDS had stimulatory effect on putidacin MAS-1 bioactivity. EDTA however, showed no effect on the bacteriocin bioactivity. It was partially purified by ammonium sulphate precipitation. SDS-PAGE showed that Putidacin MAS-1 had 15 kDa molecular weight. (author)

  1. Increasing brain angiotensin converting enzyme 2 activity decreases anxiety-like behavior in male mice by activating central Mas receptors.

    Wang, Lei; de Kloet, Annette D; Pati, Dipanwita; Hiller, Helmut; Smith, Justin A; Pioquinto, David J; Ludin, Jacob A; Oh, S Paul; Katovich, Michael J; Frazier, Charles J; Raizada, Mohan K; Krause, Eric G

    2016-06-01

    Over-activation of the brain renin-angiotensin system (RAS) has been implicated in the etiology of anxiety disorders. Angiotensin converting enzyme 2 (ACE2) inhibits RAS activity by converting angiotensin-II, the effector peptide of RAS, to angiotensin-(1-7), which activates the Mas receptor (MasR). Whether increasing brain ACE2 activity reduces anxiety by stimulating central MasR is unknown. To test the hypothesis that increasing brain ACE2 activity reduces anxiety-like behavior via central MasR stimulation, we generated male mice overexpressing ACE2 (ACE2 KI mice) and wild type littermate controls (WT). ACE2 KI mice explored the open arms of the elevated plus maze (EPM) significantly more than WT, suggesting increasing ACE2 activity is anxiolytic. Central delivery of diminazene aceturate, an ACE2 activator, to C57BL/6 mice also reduced anxiety-like behavior in the EPM, but centrally administering ACE2 KI mice A-779, a MasR antagonist, abolished their anxiolytic phenotype, suggesting that ACE2 reduces anxiety-like behavior by activating central MasR. To identify the brain circuits mediating these effects, we measured Fos, a marker of neuronal activation, subsequent to EPM exposure and found that ACE2 KI mice had decreased Fos in the bed nucleus of stria terminalis but had increased Fos in the basolateral amygdala (BLA). Within the BLA, we determined that ∼62% of GABAergic neurons contained MasR mRNA and expression of MasR mRNA was upregulated by ACE2 overexpression, suggesting that ACE2 may influence GABA neurotransmission within the BLA via MasR activation. Indeed, ACE2 overexpression was associated with increased frequency of spontaneous inhibitory postsynaptic currents (indicative of presynaptic release of GABA) onto BLA pyramidal neurons and central infusion of A-779 eliminated this effect. Collectively, these results suggest that ACE2 may reduce anxiety-like behavior by activating central MasR that facilitate GABA release onto pyramidal neurons within the

  2. Prolactin promoter gene as marker assisted selection (MAS for the control of broodiness of Kampung chicken

    Tike Sartika

    2004-12-01

    Full Text Available Preliminary research about MAS (Marker Assisted Selection was conducted to detect broodiness trait of Kampung chicken. MAS currently is very important in situations, where the accuracy of selection is low, such as, traits with low heritability, e.g. broodiness trait and egg production. Prolactin promoter was selected as a marker gene for broodiness because it plays a critical part in the neuroendocrine cascade which is triggered at the onset of broodiness. DNA samples were collected from low and highbroodiness samples on basic population (G0 each 24 samples, and from selected population (G3 each 28 samples. As control population without broody behavior was used 16 samples White Leghorn (WL chicken. Prolactin promoter gene was amplified using polymerase chain reaction (PCR. PCR product was analyzed using electrophoresis agarose gel 2%. The results showed four types of bands represent in the Kampung chicken, three types called as wild type band and one type as the WL band. The chickens with low and high broodiness on G0 generation have 75 and 87.5% of wild type band while in the G3 generation was decreased to 25 and 75%. Conclusions of the research indicated that the selected breed of the Kampung chicken on G3 generation increased WL band like White Leghorn chicken as much as 31,25% from the G0 generation.

  3. Resource-Efficient Floating-Point Data Compression Using MAS in WSN

    Maher El Assi

    2013-10-01

    Full Text Available In a wide range of applications, large amounts of f loating-point data are generated by Wireless Sensor Networks (WSNs. This data is often transferred bet ween several sensor nodes, in a multi-hop fashion, before reaching its ultimate destination (the base station. It is well known that data communications is the most energy-consuming task in sensor nodes [1]. Thi s can be a great concern when the nodes are constrained in energy. Therefore, the amount of dat a to be transferred between nodes should be reduced to save energy. In this paper, we investigate data com pression for resource-constraint WSNs; we introduce MAS as a novel adaptive lossless floating-point dat a compression algorithm for WSNs. MAS exploits the disproportionality in energy consumption between da ta transmission and processing. Simulation results, obtained from OMNeT++ and Atmel Studio, show that M AS surpasses other tested compression algorithms in terms of compression ratio, compression speed, m emory requirements and most importantly energy savings.

  4. Raman and 31P MAS NMR spectroscopic studies of lead phosphate glasses containing thorium oxide

    (PbO)0.5(P2O5)0.5 glasses in which part of the PbO/ P2O5 was replaced by ThO2 up to 10 mol% have been prepared by conventional melt quench method and characterized by Raman and 31P MAS NMR spectroscopic studies. Raman studies of these samples clearly revealed the existence of PO4 structural units having two non bridging oxygen atoms attached to phosphorus (PO22-). The 31P MAS NMR studies indicated the presence of two types of phosphorus structural units in both PbO -P2O5 and PbO-P2O5-ThO2 glasses, namely Q2 and Q1 (PO4 structural units with 2 and 1 bridging oxygen atoms respectively). Increase in the concentration of ThO2 at the expense of both PbO/P2O5 has been found to result in the increased amount of Q1 structural units of phosphorus, indicating that ThO2 acts as only a network modifier. ThO2 has been found to form the glassy phase with PbO-P2O5 system only up to 10 mol%. (author)

  5. Structural analysis of molybdo-zinc-phosphate glasses: Neutron scattering, FTIR, Raman scattering, MAS NMR studies

    Renuka, C.; Shinde, A. B.; Krishna, P. S. R.; Reddy, C. Narayana

    2016-08-01

    Vitreous samples were prepared in the xMoO3-17ZnO-(83-x) NaPO3 with 35 ≥ x ≥ 55 glass forming system by energy efficient microwave heating method. Structural evolution of the vitreous network was monitored as a function of composition by differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), Raman scattering, Magic Angle Spin Nuclear magnetic resonance (MAS NMR) and Neutron scattering. Addition of MoO3 to the ZnO-NaPO3 glass leads to a pronounced increase in glass transition temperature (Tg) suggesting a significant increase in network connectivity and strength. In order to analyze FTIR and Raman scattering, a simple structural model is presented to rationalize the experimental observations. A number of structural units are formed due to network modification, and the resulting glass may be characterized by a network polyhedral with different numbers of unshared corners. 31P MAS NMR confirms a clear distinction between structural species having 3, 2, 1, 0 bridging oxygens (BOs). Further, Neutron scattering studies were used to probe the structure of these glasses. The result suggests that all the investigated glasses have structures based on chains of four coordinated phosphate and six coordinated molybdate units, besides, two different lengths of P-O bonds in tetrahedral phosphate units that are assigned to bonds of the P-atom with terminal and bridging oxygen atoms.

  6. Lipid bilayer-bound conformation of an integral membrane beta barrel protein by multidimensional MAS NMR

    Eddy, Matthew T. [The Scripps Research Institute, Department of Integrative Structural and Computational Biology (United States); Su, Yongchao; Silvers, Robert; Andreas, Loren; Clark, Lindsay [Massachusetts Institute of Technology, Department of Chemistry (United States); Wagner, Gerhard [Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology (United States); Pintacuda, Guido; Emsley, Lyndon [Université de Lyon, Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (CNRS, ENS Lyon, UCB Lyon 1) (France); Griffin, Robert G., E-mail: rgg@mit.edu [Massachusetts Institute of Technology, Department of Chemistry (United States)

    2015-04-15

    The human voltage dependent anion channel 1 (VDAC) is a 32 kDa β-barrel integral membrane protein that controls the transport of ions across the outer mitochondrial membrane. Despite the determination of VDAC solution and diffraction structures, a structural basis for the mechanism of its function is not yet fully understood. Biophysical studies suggest VDAC requires a lipid bilayer to achieve full function, motivating the need for atomic resolution structural information of VDAC in a membrane environment. Here we report an essential step toward that goal: extensive assignments of backbone and side chain resonances for VDAC in DMPC lipid bilayers via magic angle spinning nuclear magnetic resonance (MAS NMR). VDAC reconstituted into DMPC lipid bilayers spontaneously forms two-dimensional lipid crystals, showing remarkable spectral resolution (0.5–0.3 ppm for {sup 13}C line widths and <0.5 ppm {sup 15}N line widths at 750 MHz). In addition to the benefits of working in a lipid bilayer, several distinct advantages are observed with the lipid crystalline preparation. First, the strong signals and sharp line widths facilitated extensive NMR resonance assignments for an integral membrane β-barrel protein in lipid bilayers by MAS NMR. Second, a large number of residues in loop regions were readily observed and assigned, which can be challenging in detergent-solubilized membrane proteins where loop regions are often not detected due to line broadening from conformational exchange. Third, complete backbone and side chain chemical shift assignments could be obtained for the first 25 residues, which comprise the functionally important N-terminus. The reported assignments allow us to compare predicted torsion angles for VDAC prepared in DMPC 2D lipid crystals, DMPC liposomes, and LDAO-solubilized samples to address the possible effects of the membrane mimetic environment on the conformation of the protein. Concluding, we discuss the strengths and weaknesses of the

  7. Lipid bilayer-bound conformation of an integral membrane beta barrel protein by multidimensional MAS NMR

    The human voltage dependent anion channel 1 (VDAC) is a 32 kDa β-barrel integral membrane protein that controls the transport of ions across the outer mitochondrial membrane. Despite the determination of VDAC solution and diffraction structures, a structural basis for the mechanism of its function is not yet fully understood. Biophysical studies suggest VDAC requires a lipid bilayer to achieve full function, motivating the need for atomic resolution structural information of VDAC in a membrane environment. Here we report an essential step toward that goal: extensive assignments of backbone and side chain resonances for VDAC in DMPC lipid bilayers via magic angle spinning nuclear magnetic resonance (MAS NMR). VDAC reconstituted into DMPC lipid bilayers spontaneously forms two-dimensional lipid crystals, showing remarkable spectral resolution (0.5–0.3 ppm for 13C line widths and <0.5 ppm 15N line widths at 750 MHz). In addition to the benefits of working in a lipid bilayer, several distinct advantages are observed with the lipid crystalline preparation. First, the strong signals and sharp line widths facilitated extensive NMR resonance assignments for an integral membrane β-barrel protein in lipid bilayers by MAS NMR. Second, a large number of residues in loop regions were readily observed and assigned, which can be challenging in detergent-solubilized membrane proteins where loop regions are often not detected due to line broadening from conformational exchange. Third, complete backbone and side chain chemical shift assignments could be obtained for the first 25 residues, which comprise the functionally important N-terminus. The reported assignments allow us to compare predicted torsion angles for VDAC prepared in DMPC 2D lipid crystals, DMPC liposomes, and LDAO-solubilized samples to address the possible effects of the membrane mimetic environment on the conformation of the protein. Concluding, we discuss the strengths and weaknesses of the reported

  8. X-ray fluorescent analysis on Indo-Pacific glass beads from Sungai Mas archaeological sites, Kedah, Malaysia

    Sungai Mas was an ancient port-kingdom located on West Coast of Peninsula Malaysia in a district of Kota Kuala Muda, Kedah, Malaysia. The port-kingdom evolved as an entrepot since fifth century AD and continuously visited by international trader from India, China, Middle East and Europe until eighteenth century AD. Sungai Mas was also one of the Indo-Pacific beads making centers in Southeast Asia since sixth to thirteenth century AD and also produced pottery and brick. X-ray fluorescent analysis (XRF) on Sungai Mas Indo-Pacific beads is carried out to determine whether the glass beads originated from Arikamedu, India or locally made by community in Sungai Mas. Totally, twenty-two samples of beads and beads materials assayed by XRF were chosen. Contents of nine major elements and nine trace elements, which might be present of flux, stabilizer, colorants or opacifier were examined. The elements Si, Na, K, Ca, Fe, Al, Ti, Mn, Mg, Cu, Pb, Zr, Sr, Ba, La, U, Ni and Cr were detectable in all samples. The concentration of elements found are discussed in terms of flux, silica or lead base glass, color and/or opacity of the glass beads and glass samples. The result showed that Sungai Mas produced their own Indo-Pacific beads from sixth to thirteenth century AD. (author)

  9. Role of Mas receptor in renal blood flow response to angiotensin-(1-7 in ovariectomized estradiol treated rats

    Shadan Saberi

    2016-01-01

    Full Text Available The angiotensin 1-7 (Ang 1-7, is abundantly produced in kidneys and antagonizes the function of angiotensin II through Mas receptor (MasR or other unknown mechanisms. In the current study, the role of MasR and steroid hormone estrogen on renal blood flow response to Ang 1-7 administration was investigated in ovariectomized (OV female rats. OV female Wistar-rats received estradiol (500 μg/kg/week or vehicle for two weeks. In the day of the experiment, the animals were anesthetized, cannulated, and the responses including mean arterial pressure, renal blood flow (RBF, and renal vascular resistance at the constant level of renal perfusion pressure to graded infusion of Ang 1-7 at 0, 100 and 300 ng/kg/min were determined in OV and OV estradiol-treated (OVE rats, treated with vehicle or MasR antagonist; A779. RBF response to Ang 1-7 infusion increased dose-dependently in vehicle (P dose <0.001 and A779-treated (P dose<0.01 animals. However, when MasR was blocked, the RBF response to Ang 1-7 significantly increased in OV animals compared with OVE rats (P<0.05. When estradiol was limited by ovariectomy, A779 increased RBF response to Ang 1-7 administration, while this response was attenuated in OVE animals.

  10. Low dose multi-detector CT of the chest (iLEAD Study): Visual ranking of different simulated mAs levels

    Purpose: Detailed evaluation of the lung parenchyma might be impaired by use of low dose CT as image noise increases and subsequently image quality decreases. The aim of our study was to determine the accuracy of visual perception of differences in image quality and noise at low dose chest CT. Materials and methods: Forty-four patients suffering from emphysema underwent CT (Aquilion-16, 120 kV, 150 mAs, 1 mm-collimation). Original raw data were used for simulation of 10 different mAs settings from 10 mAs to 100 mAs in 10 mAs increments. Three representative hard copy images (carina, 4 cm above, 5 cm below) were printed for evaluation of lung parenchyma (high-resolution kernel, lung window) and mediastinum (soft-kernel, soft tissue window). Ranking of expected low mAs level was performed for lung and soft tissue separately based on visual perception by three-blinded chest radiologist independently. Results were compared to the real simulated mAs. Results: The accuracy for correct ranking of the original 150 mAs scan was 89% for lung and 86% for soft tissue while it was 99% for the simulated 10 mAs for both windows. In comparison to the lowest mAs a significant error increase was found for the lung at 60-100 mAs (with error increase of 30-47%) for reader-I; 60-100 mAs for (33-64%) for reader-II and 70-100 mAs (38-57%) for reader-III. For the soft tissue: 60-150 mAs (with error increase of 28-63%) for reader-I; 50-100 mAs (35-56%) for reader-II and 50-90 mAs (35-40%) for reader-III. Conclusion: Simulated dose levels below 60 mAs (=42 mAseff) were clearly differentiated from higher dose levels by all readers. Therefore, imaging doses could be lowered down to 60 mAs without a diagnostically relevant increase in noise impairing image quality.

  11. Low dose multi-detector CT of the chest (iLEAD Study): Visual ranking of different simulated mAs levels

    Ley-Zaporozhan, Julia [Department of Diagnostic and Interventional Radiology, Ruprecht-Karls-University, Heidelberg (Germany); Department of Radiology (E010), German Cancer Research Center, Heidelberg (Germany)], E-mail: julia.leyzaporozhan@gmail.com; Ley, Sebastian [Department of Diagnostic and Interventional Radiology, Ruprecht-Karls-University, Heidelberg (Germany); Department of Radiology (E010), German Cancer Research Center, Heidelberg (Germany); Krummenauer, Frank [Clinical Epidemiology and Health Economy Unit, Carl Gustav Carus University Hospital, Dresden (Germany); Ohno, Yoshiharu [Department of Radiology, Kobe University Graduate School of Medicine, Kobe (Japan); Hatabu, Hiroto [Department of Radiology, Brigham and Women' s Hospital, Boston, MA (United States); Kauczor, Hans-Ulrich [Department of Diagnostic and Interventional Radiology, Ruprecht-Karls-University, Heidelberg (Germany)

    2010-02-15

    Purpose: Detailed evaluation of the lung parenchyma might be impaired by use of low dose CT as image noise increases and subsequently image quality decreases. The aim of our study was to determine the accuracy of visual perception of differences in image quality and noise at low dose chest CT. Materials and methods: Forty-four patients suffering from emphysema underwent CT (Aquilion-16, 120 kV, 150 mAs, 1 mm-collimation). Original raw data were used for simulation of 10 different mAs settings from 10 mAs to 100 mAs in 10 mAs increments. Three representative hard copy images (carina, 4 cm above, 5 cm below) were printed for evaluation of lung parenchyma (high-resolution kernel, lung window) and mediastinum (soft-kernel, soft tissue window). Ranking of expected low mAs level was performed for lung and soft tissue separately based on visual perception by three-blinded chest radiologist independently. Results were compared to the real simulated mAs. Results: The accuracy for correct ranking of the original 150 mAs scan was 89% for lung and 86% for soft tissue while it was 99% for the simulated 10 mAs for both windows. In comparison to the lowest mAs a significant error increase was found for the lung at 60-100 mAs (with error increase of 30-47%) for reader-I; 60-100 mAs for (33-64%) for reader-II and 70-100 mAs (38-57%) for reader-III. For the soft tissue: 60-150 mAs (with error increase of 28-63%) for reader-I; 50-100 mAs (35-56%) for reader-II and 50-90 mAs (35-40%) for reader-III. Conclusion: Simulated dose levels below 60 mAs (=42 mAs{sub eff}) were clearly differentiated from higher dose levels by all readers. Therefore, imaging doses could be lowered down to 60 mAs without a diagnostically relevant increase in noise impairing image quali0008.

  12. Study of Surface Modification Mechanism of Calcined Kaolin by MAS NMR

    杨晓杰; 邓飞皇; 张蓓

    2002-01-01

    The 29Si and 27Al in modified and unmodified calcined kaolin were res earched and compared by using MAS NMR. The result shows that the chemical shift of -106×10-6 of 29Si almost keeps unchanged after being modifie d, but 27Al cha nges obviously. The chemical shift of 5.44×10-6 and 65.69×10-6 of 27Al are sep arately shifted to 3.8×10-6-4.4×10-6 and 54.6×10-6-59.9×1 0-6 after being mod ified. And the chemical modification of kaolin is completed by linking with Al o n the surface of it.

  13. 1H MAS NMR spectra of hy- droxyl species on diatomite surface

    2001-01-01

    High spinning speed 1H magic-angle spinning nuclear magnetic resonance (1H MAS NMR) was used to detect surface hydroxyl groups of diatomite, which include isolated hydroxyl groups and hydrogen-bonded hydroxyl groups, and water adsorbed on diatomite surface that include pore water and hydrogen-bonded water. The corresponding proton chemical shifts of above species are ca. 2.0, 6.0-7.1, 4.9 and 3.0 respectively. Accompanied by thermal treatment temperature ascending, the pore water and hydrogen-bonded water are desorbed successively. As a result, the relative intensities of the peaks assigned to protons of isolat-ed hydroxyl groups and hydrogen-bonded hydroxyl groups increase gradually and reach their maxima at 1000℃. After 1100℃ calcination, the hydroxyl groups that classified to strongly hydrogen-bonded ones and the isolated hydroxyl groups condense basically. But some weakly hydrogen-bonded hydroxyl groups may still persist in the micropores.

  14. MAINTAINING THE EFFICIENCY OF MAS METHOD IN CEREALS WHILE REDUCING THE COSTS

    Odile Moullet

    2014-08-01

    Full Text Available Marker-assisted selection (MAS is a powerful tool too rarely exploited in practical breeding applications mainly because of its prohibitive costs. A new manual protocol has been developed for DNA extraction and polymerase chain reaction (PCR analyses which could increase the impact of this technology on the creation of new varieties. In this procedure, only the amount of DNA serving as template is extracted directly into PCR tubes. The method is reproducible (100 % and efficient (97.9 %. The overall cost is low in term of starting lab equipment (25000 €, chemicals and consumable materials (0.33 to 0.40 € per samples and labor (1500 sample analyses per person and per week.

  15. Paramagnet induced signal quenching in MAS-DNP experiments in frozen homogeneous solutions

    Corzilius, Björn; Andreas, Loren B.; Smith, Albert A.; Ni, Qing Zhe; Griffin, Robert G.

    2014-03-01

    The effects of nuclear signal quenching induced by the presence of a paramagnetic polarizing agent are documented for conditions used in magic angle spinning (MAS)-dynamic nuclear polarization (DNP) experiments on homogeneous solutions. In particular, we present a detailed analysis of three time constants: (1) the longitudinal build-up time constant TB for 1H; (2) the rotating frame relaxation time constant T1ρ for 1H and 13C and (3) T2 of 13C, the transverse relaxation time constant in the laboratory frame. These relaxation times were measured during microwave irradiation at a magnetic field of 5 T (140 GHz) as a function of the concentration of four polarizing agents: TOTAPOL, 4-amino-TEMPO, trityl (OX063), and Gd-DOTA and are compared to those obtained for a sample lacking paramagnetic doping. We also report the EPR relaxation time constants T1S and T2S, the DNP enhancements, ε, and the parameter E, defined below, which measures the sensitivity enhancement for the four polarizing agents as a function of the electron concentration. We observe substantial intensity losses (paramagnetic quenching) with all of the polarizing agents due to broadening mechanisms and cross relaxation during MAS. In particular, the monoradical trityl and biradical TOTAPOL induce ∼40% and 50% loss of signal intensity. In contrast there is little suppression of signal intensity in static samples containing these paramagnetic species. Despite the losses due to quenching, we find that all of the polarizing agents provide substantial gains in signal intensity with DNP, and in particular that the net enhancement is optimal for biradicals that operate with the cross effect. We discuss the possibility that much of this polarization loss can be regained with the development of instrumentation and methods to perform electron decoupling.

  16. The structural environments of cations adsorbed onto clays: A 133CsMAS NMR spectroscopic study

    Chapter One investigates the local structural environment of adsorbed cations on the mineral hectorite using 133Ca Variable-Temperature Magic-Angle-Spinning Nuclear Magnetic Resonance (VT-MAS NMR) spectroscopy. The results show that Cs on hectorite occurs in several distinctly different chemical environments, and that motional averaging of Ca between some of these sites occurs above ∼-40 degree C if water is present in the interlayer. Above ∼-10 degree C, spectra for slurries of hectorite in CsCl solutions yield two peaks, one due to Cs in solution, and the other due to Cs motionally-averaged on the clay. Below ∼-60 degree C, motional averaging of the adsorbed Cs slows sufficiently to allow resolution of two peaks representing different Cs-environments on the clay. The Stern-Gouy model is employed to explain these peaks and assign one to Cs in the Stern layer (relatively tightly bound to the basal oxygens), and the other to Cs in the Gouy diffuse layer. Between ∼-60 and ∼-10 degree C peaks for these two sites and a motionally-averaged peak are present. Cs-exchanged hectorite dehydrated at 500 degree C yields peaks for two different sites on the clay, interpreted to be highly coordinated site (probably 12), and a less coordinated site (possibly 9), both in the interlayer. Chapter II discusses 133Cs MAS NMR results for a number of other Cs-exchanged clays and the relationship of chemical and structural parameters to the 133Cs chemical shift. Increased rotational distortions of the basal oxygen sheet, total layer charge and tetrahedral Al3+ for Si4+ substitution correlate with increased deshielding of the 133Cs chemical shifts for both hydrated slurry and anhydrous samples. Correlations for the slurries are poorer because of the distances between the clay silicate and the CO in solution

  17. Sensitivity enhancement using paramagnetic relaxation in MAS solid-state NMR of perdeuterated proteins

    Linser, Rasmus; Chevelkov, Veniamin; Diehl, Anne; Reif, Bernd

    2007-12-01

    Previously, Ishii et al., could show that chelated paramagnetic ions can be employed to significantly decrease the recycle delay of a MAS solid-state NMR experiment [N.P. Wickramasinghe, M. Kotecha, A. Samoson, J. Past, Y. Ishii, Sensitivity enhancement in C-13 solid-state NMR of protein microcrystals by use of paramagnetic metal ions for optimizing H-1 T-1 relaxation, J. Magn. Reson. 184 (2007) 350-356]. Application of the method is limited to very robust samples, for which sample stability is not compromised by RF induced heating. In addition, probe integrity might be perturbed in standard MAS PRE experiments due to the use of very short duty cycles. We show that these deleterious effects can be avoided if perdeuterated proteins are employed that have been re-crystallized from D 2O:H 2O = 9:1 containing buffer solutions. The experiments are demonstrated using the SH3 domain of chicken α-spectrin as a model system. The labeling scheme allows to record proton detected 1H, 15N correlation spectra with very high resolution in the absence of heteronuclear dipolar decoupling. Cu-edta as a doping reagent yields a reduction of the recycle delay by up to a factor of 15. In particular, we find that the 1H T1 for the bulk H N magnetization is reduced from 4.4 s to 0.3 s if the Cu-edta concentration is increased from 0 mM to 250 mM. Possible perturbations like chemical shift changes or line broadening due to the paramagnetic chelate complex are minimal. No degradation of our samples was observed in the course of the experiments.

  18. Chemical profile of beans cultivars (Phaseolus vulgaris) by 1H NMR - high resolution magic angle spinning (HR-MAS);Perfil quimico de cultivares de feijao (Phaseolus vulgaris) pela tecnica de high resolution magic angle spinning (HR-MAS)

    Liao, Luciano Morais; Choze, Rafael; Cavalcante, Pedro Paulo Araujo; Santos, Suzana da Costa; Ferri, Pedro Henrique, E-mail: luciano@quimica.ufg.b [Universidade Federal de Goias (UFG), Goiania, GO (Brazil). Inst. de Quimica; Ferreira, Antonio Gilberto [Universidade Federal de Sao Carlos (UFScar), SP (Brazil). Dept. de Quimica

    2010-07-01

    The application of one-dimensional proton high-resolution magic angle spinning ({sup 1}H HR-MAS) NMR combined with a typical advantages of solid and liquid-state NMR techniques was used as input variables for the multivariate statistical analysis. In this paper, different cultivars of beans (Phaseolus vulgaris) developed and in development by EMBRAPA - Arroz e Feijao were analyzed by {sup 1}H HR-MAS, which have been demonstrated to be a valuable tool in its differentiation according chemical composition and avoid the manipulation of the samples as used in other techniques. (author)

  19. Rapid identification of osmolytes in tropical microalgae and cyanobacteria by (1)H HR-MAS NMR spectroscopy.

    Zea Obando, Claudia; Linossier, Isabelle; Kervarec, Nelly; Zubia, Mayalen; Turquet, Jean; Faÿ, Fabienne; Rehel, Karine

    2016-06-01

    In this study, we report the chemical characterization of 47 tropical microalgae and cyanobacteria by HR-MAS. The generated data confirm the interest of HR-MAS as a rapid screening technique with the major advantage of its easiness. The sample is used as powder of freeze-dried microalgae without any extraction process before acquisition. The spectral fingerprints of strains are then tested as variables for a chemotaxonomy study to discriminate cyanobacteria and dinoflagellates. The individual factor map generated by PCA analysis succeeds in separating the two groups, essentially thanks to the presence of specific carbohydrates. Furthermore, more resolved signals enable to identify many osmolytes. More precisely the characteristics δ of 2-O-alpha-D-glucosylglycerol (GG) are observed in all 21 h-MAS spectra of tropical cyanobacteria. After specific extraction, complementary analysis by 1D and 2D-NMR spectroscopies validates the identification of this osmolyte. PMID:27130130

  20. Relation Between Acid and Catalytic Properties of Chlorinated Gamma-Alumina. a 31p Mas Nmr and Ftir Investigation

    Guillaume D.

    1999-07-01

    Full Text Available In this paper, we have studied the effect of chlorine on the surface properties of gamma-alumina, especially on their acid properties. The use of FTIR spectroscopy and 31P MAS NMR of adsorbed trimethylphosphine allows to propose a chlorination mechanism. To correlate the surface properties of these chlorinated gamma-alumina with their catalytic properties, we have used a model reaction, the cracking of n-heptane under reforming conditions. The analysis of the correlation between acid properties determined by 31P MAS NMR and the catalytic results (in terms of activities and selectivities allows to identify which sites are involved in the cracking reaction.

  1. RACIONALIZACIJA ČASA MENJAVE ORODIJ ZA BRIZGANJE PLASTIČNIH MAS V PODJETJU ISKRA MEHANIZMI D.D. LIPNICA

    Oblak, Tomaž

    2010-01-01

    Spremembe, ki so stalnica današnjega časa, nastajajo zelo hitro. S tem se tudi okolje, v katerem živimo, nenehno in zelo intenzivno spreminja. Takšemu okolju se morajo hitro in nenehno prilagajati tudi podjetja, če si želijo zagotoviti svoj dolgoročni obstoj. V diplomski nalogi je predstavljeno nekaj osnov o brizganju plastičnih mas in preoblikovanje sistema menjav orodij na strojih za brizganje plastičnih mas s pomočjo uporabe metode SMED. V nalogi so predstavljene prednosti, ki jih met...

  2. Bulk carbohydrate grain filling of barley ß-glucan mutants studied by 1H HR MAS NMR

    Seefeldt, Helene Fast; Larsen, Flemming Hofmann; Viereck, Nanna;

    2008-01-01

    Temporal and genotypic differences in bulk carbohydrate accumulation in three barley genotypes differing in the content of mixed linkage β-(1→3),(1→4)-D-glucan (β-glucan) and starch were investigated using proton high-resolution, magic angle spinning, nuclear magnetic resonance (1H HR MAS NMR......) during grain filling. For the first time, 1H HR MAS NMR spectra of flour from immature barley seeds are analyzed. Spectral assignments are made using two-dimensional (2D) NMR methods. Both α- and β-glucan biosynthesis were characterized by inspection of the spectra as well as by calibration to the...

  3. The Magellan Adaptive Secondary VisAO Camera: Diffraction- Limited Broadband Visible Imaging and 20mas Fiber Array IFS

    Kopon, Derek; Close, Laird M; Males, Jared R.; Gasho, Victor; Follette, Katherine

    2010-01-01

    The Magellan Adaptive Secondary AO system, scheduled for first light in the fall of 2011, will be able to simultaneously perform diffraction limited AO science in both the mid-IR, using the BLINC/MIRAC4 10\\{mu}m camera, and in the visible using our novel VisAO camera. The VisAO camera will be able to operate as either an imager, using a CCD47 with 8.5 mas pixels, or as an IFS, using a custom fiber array at the focal plane with 20 mas elements in its highest resolution mode. In imaging mode, t...

  4. Inventario de especies domésticas de un mas de la comarca de La Selva, Catalunya (1803-2013)

    Fàbregas i Comadran, Xavier

    2013-01-01

    Se comparan diacrónicamente los censos de las especies domésticas de un mas de la comarca de la Selva (Catalunya) mediante un inventario notarial de 1803, un informante oral de 1960 y los censos oficiales de 2013. Aunque es un mas pobre por estar situado en una finca de tamaño medio, pero de tipología forestal, la mayor variedad y cantidad de ganado censada corresponde a la década de 1960. Congreso Iberoamericano de Historia de la Veterinaria. Madrid, 10è : 2013

  5. ¿Hacia un partido “tradicional”? Un análisis del cambio organizativo interno en el Movimiento Al Socialismo (MAS) en Bolivia

    Harten, Sven

    2007-01-01

    Since the electoral victory of Evo Morales and his Movimiento Al Socialismo (MAS) in December 2005, a lot has been written about the first indigenous president in Bolivia. However, less attention has been paid to study his “political instrument” (i.e. MAS). The present paper intends, therefore, to analyse qualitatively the internal organization of MAS with an emphasis on a comparative perspective between the party before and after 2004. Using participant observation and in-depth interviews, t...

  6. Proton chemical shift tensors determined by 3D ultrafast MAS double-quantum NMR spectroscopy

    Zhang, Rongchun; Mroue, Kamal H.; Ramamoorthy, Ayyalusamy, E-mail: ramamoor@umich.edu [Biophysics and Department of Chemistry, The University of Michigan, Ann Arbor, Michigan 48109-1055 (United States)

    2015-10-14

    Proton NMR spectroscopy in the solid state has recently attracted much attention owing to the significant enhancement in spectral resolution afforded by the remarkable advances in ultrafast magic angle spinning (MAS) capabilities. In particular, proton chemical shift anisotropy (CSA) has become an important tool for obtaining specific insights into inter/intra-molecular hydrogen bonding. However, even at the highest currently feasible spinning frequencies (110–120 kHz), {sup 1}H MAS NMR spectra of rigid solids still suffer from poor resolution and severe peak overlap caused by the strong {sup 1}H–{sup 1}H homonuclear dipolar couplings and narrow {sup 1}H chemical shift (CS) ranges, which render it difficult to determine the CSA of specific proton sites in the standard CSA/single-quantum (SQ) chemical shift correlation experiment. Herein, we propose a three-dimensional (3D) {sup 1}H double-quantum (DQ) chemical shift/CSA/SQ chemical shift correlation experiment to extract the CS tensors of proton sites whose signals are not well resolved along the single-quantum chemical shift dimension. As extracted from the 3D spectrum, the F1/F3 (DQ/SQ) projection provides valuable information about {sup 1}H–{sup 1}H proximities, which might also reveal the hydrogen-bonding connectivities. In addition, the F2/F3 (CSA/SQ) correlation spectrum, which is similar to the regular 2D CSA/SQ correlation experiment, yields chemical shift anisotropic line shapes at different isotropic chemical shifts. More importantly, since the F2/F1 (CSA/DQ) spectrum correlates the CSA with the DQ signal induced by two neighboring proton sites, the CSA spectrum sliced at a specific DQ chemical shift position contains the CSA information of two neighboring spins indicated by the DQ chemical shift. If these two spins have different CS tensors, both tensors can be extracted by numerical fitting. We believe that this robust and elegant single-channel proton-based 3D experiment provides useful atomistic

  7. New perspectives of 19F MAS NMR in the characterization of amorphous forms of atorvastatin in dosage formulations

    Brus, Jiří; Urbanová, Martina; Šeděnková, Ivana; Brusová, H.

    2011-01-01

    Roč. 409, 1/2 (2011), s. 62-74. ISSN 0378-5173 R&D Projects: GA MŠk 2B08021 Institutional research plan: CEZ:AV0Z40500505 Keywords : 19F MAS NMR * factor analysis * polymorphism Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 3.350, year: 2011

  8. [Effect of Astragali Radix in improving early renal damage in metabolic syndrome rats through ACE2/Mas pathway].

    Wang, Qiong-ying; Liang, Wei; Jiang, Cheng; Li, Ning-yin; Xu, Han; Yang, Mi-na; Lin, Xin; Yu, Heng; Chang, Peng; Yu, Jing

    2015-11-01

    To study the expression of angiotensin converting enzyme 2 (ACE2) and angiotensin (Ang) 1-7 specific receptor Mas protain in renal blood vessels of metabolic syndrome ( MS) rats and its anti-oxidative effect. A total of 80 male SD rats were divided into four groups: the normal control group (NC, the same volume of normal saline), the MS group (high fat diet), the MS + Astragali Radix group (MS + HQ, 6 g x kg(-1) x d(-1) in gavage) and the MS + Valsartan group (MS + XST, 30 mg x kg(-1) x d(-1) in gavage). After four weeks of intervention, their general indexes, biochemical indexes and blood pressure were measured; plasma and renal tissue Ang II, malondialdehyde (MDA) and superoxide demutase (SOD) levels were measured with radioimmunoassay. The protein expressions of Mas receptor, AT1R, ACE and ACE2 were detected by western blot analysis. According to the result, compared with the NC group, the MS group and the MS + HQ group showed significant increases in systolic and diastolic pressures, body weight, fasting glucose, fasting insulin, triglycerides, free fatty acid and Ang II level of MS rats (P protein expressions in renal tissues of the MS group were higher than that in the NC group, but with lower ACE2 and -Mas receptor expressions (all P < 0.05). Compared with the MS group, the MS + HQ group showed significant increase in Mas receptor expression in renal tissues, whereas the MS + XST group showed notable decrease in AT1R (all P < 0.05). In conclusion, Astragali Radix can increase the Mas receptor expressions in renal tissues, decrease ACE expression and change local Ang II, MDA, NO and SOD in kidneys, so as to protect early damages in renal tissues. PMID:27071265

  9. SiC nanowires reinforced MAS joint of SiC coated carbon/carbon composites to LAS glass ceramics

    Graphical abstract: Schematic cross-section images of the samples with SiC nanowires and assembly sequence for the raw materials through the process of the hot-pressing method. Highlights: ► The SiC nanowires were firstly used as porous layer in the middle of the joint. ► The shear strength of the joint with SiC nanowires was largely improved. ► A new mode of fracture was proposed. - Abstract: In order to improve the shear strength of the joints of carbon/carbon (C/C) composites to lithium aluminum silicate (LAS) glass ceramics, SiC coating and magnesium aluminum silicate (MAS) glass ceramics were used as transition layer and middle layer, respectively, and high quality SiC nanowires were applied as the reinforcement materials in MAS. The SiC nanowires reinforced MAS joint of SiC coated C/C composites to LAS glass ceramics was prepared by a three-step technique of pack cementation, CVD and hot-pressing. The microstructures of the as-prepared joints were characterized by SEM and EDS, and the shear strength of the joints was also examined. The shear strength of the SiC–MAS joint increased from 24.0 ± 2.0 MPa to 35.5 ± 5.5 MPa after adding SiC nanowires in MAS. The load decreases in step-style but not perpendicularly after the maximum value, which demonstrates good toughness of the joint with SiC nanowire porous layer.

  10. Observations of radio-quiet quasars at 10mas resolution by use of gravitational lensing

    Jackson, Neal; Roberts, Carl; Sluse, Dominique; Stacey, Hannah; Vives-Arias, Hector; Wucknitz, Olaf; Volino, Filomena

    2015-01-01

    We present VLA detections of radio emission in four four-image gravitational lens systems with quasar sources: HS0810+2554, RXJ0911+0511, HE0435$-$1223 and SDSSJ0924+0219, and e-MERLIN observations of two of the systems. The first three are detected at a high level of significance, and SDSS J0924+0219 is detected. HS0810+2554 is resolved, allowing us for the first time to achieve 10-mas resolution of the source frame in the structure of a radio quiet quasar. The others are unresolved or marginally resolved. All four objects are among the faintest radio sources yet detected, with intrinsic flux densities in the range 1-5$\\mu$Jy; such radio objects, if unlensed, will only be observable routinely with the Square Kilometre Array. The observations of HS0810+2554, which is also detected with e-MERLIN, strongly suggest the presence of a mini-AGN, with a radio core and milliarcsecond scale jet. The flux densities of the lensed images in all but HE0435-1223 are consistent with smooth galaxy lens models without the req...

  11. Dynamic scheduling model of computing resource based on MAS cooperation mechanism

    JIANG WeiJin; ZHANG LianMei; WANG Pu

    2009-01-01

    Allocation of grid resources aims at improving resource utility and grid application performance. Currently, the algorithms proposed for this purpose do not fit well the autonomic, dynamic, distributive and heterogeneous features of the grid environment. According to MAS (multi-agent system) cooperation mechanism and market bidding game rules, a model of allocating allocation of grid resources based on market economy is introduced to reveal the relationship between supply and demand. This model can make good use of the studying and negotiating ability of consumers' agent and takes full consideration of the consumer's behavior, thus rendering the application and allocation of resource of the consumers rational and valid. In the meantime, the utility function of consumer Is given; the existence and the uniqueness of Nash equilibrium point in the resource allocation game and the Nash equilibrium solution are discussed. A dynamic game algorithm of allocating grid resources is designed. Experimental results demonstrate that this algorithm diminishes effectively the unnecessary latency, improves significantly the smoothness of response time, the ratio of throughput and resource utility, thus rendering the supply and demand of the whole grid resource reasonable and the overall grid load balanceable.

  12. 31P MAS-NMR of human erythrocytes: independence of cell volume from angular velocity.

    Kuchel, P W; Bubb, W A; Ramadan, S; Chapman, B E; Philp, D J; Coen, M; Gready, J E; Harvey, P J; McLean, A J; Hook, J

    2004-09-01

    31P magic angle spinning NMR (MAS-NMR) spectra were obtained from suspensions of human red blood cells (RBCs) that contained the cell-volume-sensitive probe molecule, dimethyl methylphosphonate (DMMP). A mathematical representation of the spectral-peak shape, including the separation and width-at-half-height in the 31P NMR spectra, as a function of rotor speed, enabled us to explore the extent to which a change in cell volume would be reflected in the spectra if it occurred. We concluded that a fractional volume change in excess of 3% would have been detected by our experiments. Thus, the experiments indicated that the mean cell volume did not change by this amount even at the highest spinning rate of 7 kHz. The mean cell volume and intracellular 31P line-width were independent of the packing density of the cells and of the initial cell volume. The relationship of these conclusions to other non-NMR studies of pressure effects on cells is noted. PMID:15334588

  13. Characterization of cornelian cherry (Cornus mas L. genotypes - genetic resources for food production in Czech Republic

    Sochor Jiri

    2014-01-01

    Full Text Available The aim of the study was to determine and compare both technological properties and polyphenol content in fruits of eight cornellian cherry (Cornus mas L. cultivars from Czech Republic. The fruits of cultivar ´Vydubeckij´ had the highest dry matter (17.4% and soluble solid (15.8% content among searched cultivars. The highest crude protein and phosphorus values were determined in the fruits of the ´Titus´ cultivar as 10.9 g of crude protein and 435 mg of phosphorus for per kg fresh weight (FW base. The ´Elegantnyj´ and ´Vydubeckij´ cultivars are found to be more suitable for canning industry. The fruits of the ´Vydubeckij´ cultivar contained 10.8 g of pectin and 22.4 g of citric acid per kg fresh weight base, and could be important for technological use because of their excellent gelling ability. ´Devin´,´Vydubeckij´ and ´Titus´ cultivars had the most valuable source of chlorogenic acid (135.6, 110.9 and 115.1 mg per 100 g FW, respectively and quercetin (24.9, 25.2 and 24.2 mg per 100 g FW, respectively. This work should contribute to the popularization of this fruit specie as a valuable source of technological parameters for human nutrition and the canning industry.

  14. Identification of ancient Olea europaea L. and Cornus mas L. seeds by DNA barcoding.

    Gismondi, Angelo; Rolfo, Mario Federico; Leonardi, Donatella; Rickards, Olga; Canini, Antonella

    2012-07-01

    The analysis of ancient DNA (aDNA) provides archaeologists and anthropologists with innovative, scientific and accurate data to study and understand the past. In this work, ancient seeds, found in the "Mora Cavorso" archaeological site (Latium, Central Italy), were analyzed to increase information about Italian Neolithic populations (plant use, agriculture, diet, trades, customs and ecology). We performed morphological and genetic techniques to identify fossil botanical species. In particular, this study also suggests and emphasizes the use of DNA barcode method for ancient plant sample analysis. Scanning electron microscope (SEM) observations showed seed compact structure and irregular surface but they did not permit a precise nor empirical classification: so, a molecular approach was necessary. DNA was extracted from ancient seeds and then it was used, as template, for PCR amplifications of standardized barcode genes. Although aDNA could be highly degraded by the time, successful PCR products were obtained, sequenced and compared to nucleotide sequence databases. Positive outcomes (supported by morphological comparison with modern seeds, geographical distribution and historical data) indicated that seeds could be identified as belonging to two plant species: Olea europaea L. and Cornus mas L. PMID:22847014

  15. The Neuroprotective Effect of Cornus mas on Brain Tissue of Wistar Rats

    Renata Francik

    2014-01-01

    Full Text Available Cornelian cherry (Cornus mas is a valuable source of phenolic antioxidants. Flavonoid derivatives as nonenzymatic antioxidants are important in the pathophysiology of many diseases including neurological disorders (e.g., Alzheimer’s disease or heart disease. In this study, we examined the effect of an addition of freeze-dried fruit of cornelian cherry on three types of diets: control diet, fructose diet, and diet enriched in fats (high-fat diet. This effect was studied by determining the following antioxidant parameters in both brain tissue and plasma in rats: catalase, ferric reducing ability of plasma, paraoxonase, protein carbonyl groups, and free thiol groups. Results indicate that both fructose diet and high-fat diet affect the antioxidant capacity of the organism. Furthermore, an addition of cornelian cherry resulted in increased activity of catalase in brain tissue, while in plasma it caused the opposite effect. In turn, with regard to paraoxonase activity in both brain tissue and plasma, it had a stimulating effect. Adding cornelian cherry to the tested diets increased the activity of PON in both tested tissues. Moreover, protective effect of fruits of this plant was observed in the process of oxidation of proteins by decreasing levels of protein carbonyl groups and thiol groups in brain tissue as well as in plasma.

  16. Nutritional value of Rosa spp. l. and Cornus mas l. fruits, as affected by storage conditions

    Craita-Maria Rosu

    2011-12-01

    Full Text Available A comparison between two storage methods (freezing and drying of fruits, in terms of ascorbic acid, carotenes, total sugar and protein content in Rosa spp. L. species and Cornus mas L. was performed. In the dried rose hips, the major losses were registered at the level of ascorbic acid content (32.04–50.25 %, followed by carotenes (30.85–52.08 %, total sugar (21.57–34.6 % and protein content (21.33–46.89 %. The freezing method resulted in a better preservation of ascorbic acid (only 19.80–29.21 % decrease and total sugar content (3.41–12.94 % increase. In the preserved cornelian cherry fruits, no statistically significant differences were registered between fruits categories, except carotene content, which was dramatically decreased in dried fruits (88.23 %. For cornelian cherry fruits, both storage methods induced a decrease of ascorbic acid and protein contents (57.60 and 46.32 % and an increase of total sugar level (37.60 %.

  17. Tendencias clave para el tercer gobierno del MAS, Bolivia 2015-2019

    Xavier Albó

    2016-03-01

    Esta presentación tiene como trasfondo el Plan Nacional de Desarrollo y pretende resaltar algunos de los logros del presidente Evo Morales y los desafíos que enfrenta en su tercer gobierno (2015-2019. Comienza revisando información demográfica básica, según el Censo de 2012, para luego centrarse en la economía: los hidrocarburos, la minería, la producción agropecuaria y la ecología. El boom de la quinua, la expansión de la frontera agrícola y la suspensión de la construcción de una carretera que atraviese el TIPNIS son algunos de los temas que se discuten. Internacionalmente, hay motivos de optimismo con relación a la demanda marítima de Bolivia, mientras que, a nivel nacional, el MAS aseguró una victoria parlamentaria en el año 2014. Las reformas menos exitosas son las del órgano judicial. La presentación termina revisando los movimientos sociales que permitieron la formación la de Asamblea Constituyente y el asunto de la autonomía municipal, departamental e indígena.

  18. PENGEMBANGAN EKOWISATA BERBASIS KERAKYATAN DI BANJAR NYUH KUNING, DESA MAS, UBUD

    AGUNG SRI SULISTYAWATI

    2015-06-01

    Full Text Available The objective of this study is (1 to identify the potential of Banjar Nyuh Kuning Mas Village, Ubud has to offer as means of attraction in ecotourism which may be accomplished by identifying physical and non physical potential. (2 to develope a community based strategy in ecotourism. The sampling technique used is purposive sampling and accidental sampling. Data collection is accomplished through observatioan, questionaire, focus group discussion and documentation. Data analysis is by qualitative descriptive and SWOT. The result of this study is that Banjar Nyuh Kuning has the potential to be developed as an ecotourism on the basis of physical and non physical grounds. The perception of the community and tourists are in agreement to the developement of ecotourism activity which incorporate local community, nature oriented activity and has the potential to increase their income, thus resulting in a destination for leisure and recreation. There are a few results of alternative strategies such as; development strategies of ecotourism products, development strategies of human resources, development strategies of management and strategies in upgrading security. To make Banjar Nyuh Kuning as an attraction for ecotourism activity which is based on nature then the strategy for development has to be efficient and limited. Further study has to be taken for the marketing aspect and managing ecotourism object to further the developent in a comprehensive manner.

  19. Investigations of Li-containing SiCN(O) ceramics via 7Li MAS NMR.

    Gumann, Sina; Nestle, Nikolaus; Liebau-Kunzmann, Verena; Riedel, Ralf

    2007-04-01

    Lithium-containing silicon (oxy)carbonitride ceramics (SiCN(O):Li) were synthesized via precursor-to-ceramic-transformation of Li-containing (poly)silazanes. The precursors were obtained by lithiation of 2,4,6-trimethyl-2,4,6-trivinylcyclotrisilazane with n-butyllithium and by reaction of a commercial poly(organosilazane) VL20 with metallic lithium. The annealing treatment was carried out at temperatures between 200 and 1400 degrees C in argon (DeltaT=200 degrees C) and yielded Li-containing silicon (oxy)carbonitride. X-ray powder diffraction revealed that the resulting SiCN(O):Li ceramics were basically amorphous up to temperatures of 1000 degrees C and formed LiSi(2)N(3), graphite and silicon carbide as crystalline phases at higher temperatures. (7)Li MAS NMR spectroscopy was carried out to investigate the structure of the Li-containing phases and to study the reaction path of metallic Li with polysilazane. Based on the NMR spectra, there is almost no difference found in the chemical shift of the SiCN(O):Li ceramics obtained at different temperatures. Accordingly, Li is assigned to be mainly coordinated to N and O present as contaminant element. Relaxation time measurements showed that the most mobile Li(+) species seems to be present in the product obtained in the pyrolysis temperature range between 600 and 1000 degrees C. PMID:17418540

  20. LyMAS: Predicting large-scale Lyα forest statistics from the dark matter density field

    We describe Lyα Mass Association Scheme (LyMAS), a method of predicting clustering statistics in the Lyα forest on large scales from moderate-resolution simulations of the dark matter (DM) distribution, with calibration from high-resolution hydrodynamic simulations of smaller volumes. We use the 'Horizon-MareNostrum' simulation, a 50 h –1 Mpc comoving volume evolved with the adaptive mesh hydrodynamic code RAMSES, to compute the conditional probability distribution P(Fs |δ s) of the transmitted flux Fs , smoothed (one-dimensionally, 1D) over the spectral resolution scale, on the DM density contrast δ s, smoothed (three-dimensionally, 3D) over a similar scale. In this study we adopt the spectral resolution of the SDSS-III Baryon Oscillation Spectroscopic Survey (BOSS) at z = 2.5, and we find optimal results for a DM smoothing length σ = 0.3 h –1 Mpc (comoving). In its simplest form, LyMAS draws randomly from the hydro-calibrated P(Fs |δ s) to convert DM skewers into Lyα forest pseudo-spectra, which are then used to compute cross-sightline flux statistics. In extended form, LyMAS exactly reproduces both the 1D power spectrum and one-point flux distribution of the hydro simulation spectra. Applied to the MareNostrum DM field, LyMAS accurately predicts the two-point conditional flux distribution and flux correlation function of the full hydro simulation for transverse sightline separations as small as 1 h –1 Mpc, including redshift-space distortion effects. It is substantially more accurate than a deterministic density-flux mapping (Fluctuating Gunn-Peterson Approximation), often used for large-volume simulations of the forest. With the MareNostrum calibration, we apply LyMAS to 10243 N-body simulations of a 300 h –1 Mpc and 1.0 h –1 Gpc cube to produce large, publicly available catalogs of mock BOSS spectra that probe a large comoving volume. LyMAS will be a powerful tool for interpreting 3D Lyα forest data, thereby transforming

  1. X-ray and MAS NMR characterization of the thermal transformation of Li(Na)-Y zeolite to lithium aluminosilicates

    The high temperature thermal transformation of Li-exchanged Na-Y zeolite has been investigated by X-ray diffraction and /sup 29/Si MAS NMR studies. At 7000C, the zeolite was transformed into an amorphous phase and upon further heating to 8000C, formation of lithium aluminosilicate with high-quartz structure, in addition to an amorphous phase, was noted. When heated above 9000C, the high-quartz structure was transformed into a β-spodumene related solid solution. X-ray and MAS NMR studies indicate the β-spodumene solid solution formed has the composition close to (Li/sub 0.23/Na/sub 0.06/)A iota /sub 0.29/Si/sub 0.71/O/sub 2/, which is in agreement with chemical analysis

  2. Characterization of solid polymer dispersions of active pharmaceutical ingredients by 19F MAS NMR and factor analysis

    Urbanova, Martina; Brus, Jiri; Sedenkova, Ivana; Policianova, Olivia; Kobera, Libor

    In this contribution the ability of 19F MAS NMR spectroscopy to probe structural variability of poorly water-soluble drugs formulated as solid dispersions in polymer matrices is discussed. The application potentiality of the proposed approach is demonstrated on a moderately sized active pharmaceutical ingredient (API, Atorvastatin) exhibiting extensive polymorphism. In this respect, a range of model systems with the API incorporated in the matrix of polvinylpyrrolidone (PVP) was prepared. The extent of mixing of both components was determined by T1(1H) and T1ρ(1H) relaxation experiments, and it was found that the API forms nanosized domains. Subsequently it was found out that the polymer matrix induces two kinds of changes in 19F MAS NMR spectra. At first, this is a high-frequency shift reaching 2-3 ppm which is independent on molecular structure of the API and which results from the long-range polarization of the electron cloud around 19F nucleus induced by electrostatic fields of the polymer matrix. At second, this is broadening of the signals and formation of shoulders reflecting changes in molecular arrangement of the API. To avoid misleading in the interpretation of the recorded 19F MAS NMR spectra, because both the contributions act simultaneously, we applied chemometric approach based on multivariate analysis. It is demonstrated that factor analysis of the recorded spectra can separate both these spectral contributions, and the subtle structural differences in the molecular arrangement of the API in the nanosized domains can be traced. In this way 19F MAS NMR spectra of both pure APIs and APIs in solid dispersions can be directly compared. The proposed strategy thus provides a powerful tool for the analysis of new formulations of fluorinated pharmaceutical substances in polymer matrices.

  3. TRPA1 is required for histamine-independent, Mas-related G protein-coupled receptor-mediated itch

    Wilson, Sarah R.; Kristin A Gerhold; Bifolck-Fisher, Amber; Liu, Qin; Patel, Kush N.; Dong, Xinzhong; Bautista, Diana M.

    2011-01-01

    SUMMARY Itch, the unpleasant sensation that evokes a desire to scratch, accompanies numerous skin and nervous system disorders. In many cases, pathological itch is insensitive to antihistamine treatment. Recent studies have identified members of the Mas-related GPCR (Mrgpr) family that are activated by mast cell mediators and promote histamine-independent itch. MrgprA3 and MrgprC11 act as receptors for the pruritogens chloroquine and BAM8–22, respectively. However, the signaling pathways and ...

  4. Polydisperse methyl β-cyclodextrin–epichlorohydrin polymers: variable contact time 13C CP-MAS solid-state NMR characterization

    Isabelle Mallard

    2015-12-01

    Full Text Available The polymerization of partially methylated β-cyclodextrin (CRYSMEB with epichlorohydrin was carried out in the presence of a known amount of toluene as imprinting agent. Three different preparations (D1, D2 and D3 of imprinted polymers were obtained and characterized by solid-state 13C NMR spectroscopy under cross-polarization magic angle spinning (CP-MAS conditions. The polymers were prepared by using the same synthetic conditions but with different molar ratios of imprinting agent/monomer, leading to morphologically equivalent materials but with different absorption properties. The main purpose of the work was to find a suitable spectroscopic descriptor accounting for the different imprinting process in three homogeneous polymeric networks. The polymers were characterized by studying the kinetics of the cross-polarization process. This approach is based on variable contact time CP-MAS spectra, referred to as VCP-MAS. The analysis of the VCP-MAS spectra provided two relaxation parameters: TCH (the CP time constant and T1ρ (the proton spin-lattice relaxation time in the rotating frame. The results and the analysis presented in the paper pointed out that TCH is sensitive to the imprinting process, showing variations related to the toluene/cyclodextrin molar ratio used for the preparation of the materials. Conversely, the observed values of T1ρ did not show dramatic variations with the imprinting protocol, but rather confirmed that the three polymers are morphologically similar. Thus the combined use of TCH and T1ρ can be helpful for the characterization and fine tuning of imprinted polymeric matrices.

  5. Polydisperse methyl β-cyclodextrin-epichlorohydrin polymers: variable contact time (13)C CP-MAS solid-state NMR characterization.

    Mallard, Isabelle; Baudelet, Davy; Castiglione, Franca; Ferro, Monica; Panzeri, Walter; Ragg, Enzio; Mele, Andrea

    2015-01-01

    The polymerization of partially methylated β-cyclodextrin (CRYSMEB) with epichlorohydrin was carried out in the presence of a known amount of toluene as imprinting agent. Three different preparations (D1, D2 and D3) of imprinted polymers were obtained and characterized by solid-state (13)C NMR spectroscopy under cross-polarization magic angle spinning (CP-MAS) conditions. The polymers were prepared by using the same synthetic conditions but with different molar ratios of imprinting agent/monomer, leading to morphologically equivalent materials but with different absorption properties. The main purpose of the work was to find a suitable spectroscopic descriptor accounting for the different imprinting process in three homogeneous polymeric networks. The polymers were characterized by studying the kinetics of the cross-polarization process. This approach is based on variable contact time CP-MAS spectra, referred to as VCP-MAS. The analysis of the VCP-MAS spectra provided two relaxation parameters: T CH (the CP time constant) and T 1ρ (the proton spin-lattice relaxation time in the rotating frame). The results and the analysis presented in the paper pointed out that T CH is sensitive to the imprinting process, showing variations related to the toluene/cyclodextrin molar ratio used for the preparation of the materials. Conversely, the observed values of T 1ρ did not show dramatic variations with the imprinting protocol, but rather confirmed that the three polymers are morphologically similar. Thus the combined use of T CH and T 1ρ can be helpful for the characterization and fine tuning of imprinted polymeric matrices. PMID:26877800

  6. Studi Aplikasi Total Productive Maintenance Untuk Peningkatan Efisiensi Produksi Dengan Metode Overall Equipment Effectiveness Di PT. Rolimex Kimia Nusa Mas

    Mupianda, Ahmad

    2011-01-01

    PT. Rolimex Kimia Nusa Mas merupakan sebuah perusahaan yang memproduksi pupuk CIRP (Crismes Island Rock Phosphate) yang juga tidak terlepas dari masalah yang berkaitan dengan efektifitas mesin/peralatan yang diakibatkan oleh six big losses tersebut. Hal ini dapat terlihat dengan frekuensi kerusakan yang terjadi pada mesin/peralatan karena kerusakan tersebut target produksi tidak tercapai. Oleh karena itulah diperlukan langkah-langkah yang efektif dan efisien dalam pemeliharaan mesin/peralatan...

  7. Evaluación ecotoxicológica del FitoMas-H en lombriz de tierra y abejas

    Axel Mancebo

    2008-01-01

    Full Text Available Aunque constituye una necesidad el aumento de las producciones agrícolas, y actualmente se estimula la disminución del uso de productos químicos, mediante su sustitución por fertilizantes orgánicos y biológicos, es preciso evaluar el impacto de éstos sobre las especies del ecosistema en el cual se liberan. Con el objetivo de realizar la evaluación ecotoxicológica en sistemas terrestres del FitoMas-H, se realizaron los ensayos de toxicidad aguda en lombriz de tierra (Eisenia foetida y de toxicidad aguda por contacto en abejas (Apis mellifera. Se evaluó una concentración de 1000 mg de FitoMas-H /Kg de suelo artificial en el primer caso, mientras en el ensayo con abejas se utilizó una dosis de 100 g de FitoMas - H/animal. El ensayo en lombrices culminó con un 97,5 % de supervivencia en el grupo Tratado, sin diferencias significativas en la mortalidad y la variación de biomasa del grupo Control contra el Tratado. Por otra parte, el estudio por contacto en abejas terminó con una supervivencia del 95.45%, sin diferencias entre la mortalidad del grupo Control contra el Tratado. En ambos casos, se pudo concluir que la exposición al FitoMas-H no produce efectos tóxicos en los invertebrados terrestres utilizados.

  8. Characterization of solid polymer dispersions of active pharmaceutical ingredients by 19F MAS NMR and factor analysis

    Urbanová, Martina; Brus, Jiří; Šeděnková, Ivana; Policianová, Olivia; Kobera, Libor

    2013-01-01

    Roč. 100, 1 January (2013), s. 59-66. ISSN 1386-1425 R&D Projects: GA ČR GPP106/11/P426; GA MŠk 2B08021 Institutional research plan: CEZ:AV0Z40500505 Institutional support: RVO:61389013 Keywords : solid-state NMR * factor analysis * 19F MAS NMR Subject RIV: FR - Pharmacology ; Medidal Chemistry Impact factor: 2.129, year: 2013

  9. Polydisperse methyl β-cyclodextrin–epichlorohydrin polymers: variable contact time 13C CP-MAS solid-state NMR characterization

    Mallard, Isabelle; Baudelet, Davy; Castiglione, Franca; Ferro, Monica; Panzeri, Walter; Ragg, Enzio

    2015-01-01

    Summary The polymerization of partially methylated β-cyclodextrin (CRYSMEB) with epichlorohydrin was carried out in the presence of a known amount of toluene as imprinting agent. Three different preparations (D1, D2 and D3) of imprinted polymers were obtained and characterized by solid-state 13C NMR spectroscopy under cross-polarization magic angle spinning (CP-MAS) conditions. The polymers were prepared by using the same synthetic conditions but with different molar ratios of imprinting agent/monomer, leading to morphologically equivalent materials but with different absorption properties. The main purpose of the work was to find a suitable spectroscopic descriptor accounting for the different imprinting process in three homogeneous polymeric networks. The polymers were characterized by studying the kinetics of the cross-polarization process. This approach is based on variable contact time CP-MAS spectra, referred to as VCP-MAS. The analysis of the VCP-MAS spectra provided two relaxation parameters: T CH (the CP time constant) and T 1ρ (the proton spin-lattice relaxation time in the rotating frame). The results and the analysis presented in the paper pointed out that T CH is sensitive to the imprinting process, showing variations related to the toluene/cyclodextrin molar ratio used for the preparation of the materials. Conversely, the observed values of T 1ρ did not show dramatic variations with the imprinting protocol, but rather confirmed that the three polymers are morphologically similar. Thus the combined use of T CH and T 1ρ can be helpful for the characterization and fine tuning of imprinted polymeric matrices. PMID:26877800

  10. Cross Religious and Social Interaction: A Case Study of Muslims and Buddhists in Kampung Tendong, Pasir Mas, Kelantan

    Engku Ahmad Zaki Engku Alwi; Zuriati Binti Mohd Rashid

    2011-01-01

    The main objective of this study is to explore the quality of interaction between Muslims and Buddhists in Kampung Tendong. The researcher prepared one relevant indicator to measure quality interaction, that is religious understandings. Using a convenience sampling technique, a total of one hundred and forty (140) respondents were drawn from Muslims and Buddhists of Kampong Tendong, Pasir Mas, Kelantan. The sample size together with the above-mentioned indicators showed that the quality of in...

  11. 15N Photo-CIDNP MAS NMR To Reveal Functional Heterogeneity in Electron Donor of Different Plant Organisms

    Janssen, Geertje J.; Roy, Esha; Matysik, Jörg; Alia, A.

    2011-01-01

    In plants and cyanobacteria, two light-driven electron pumps, photosystems I and II (PSI, PSII), facilitate electron transfer from water to carbon dioxide with quantum efficiency close to unity. While similar in structure and function, the reaction centers of PSI and PSII operate at widely different potentials with PSI being the strongest reducing agent known in living nature. Photochemically induced dynamic nuclear polarization (photo-CIDNP) in magic-angle spinning (MAS) nuclear magnetic res...

  12. The Magellan Adaptive Secondary VisAO Camera: Diffraction- Limited Broadband Visible Imaging and 20mas Fiber Array IFS

    Kopon, Derek; Males, Jared R; Gasho, Victor; Follette, Katherine

    2010-01-01

    The Magellan Adaptive Secondary AO system, scheduled for first light in the fall of 2011, will be able to simultaneously perform diffraction limited AO science in both the mid-IR, using the BLINC/MIRAC4 10\\{mu}m camera, and in the visible using our novel VisAO camera. The VisAO camera will be able to operate as either an imager, using a CCD47 with 8.5 mas pixels, or as an IFS, using a custom fiber array at the focal plane with 20 mas elements in its highest resolution mode. In imaging mode, the VisAO camera will have a full suite of filters, coronagraphic focal plane occulting spots, and SDI prism/filters. The imaging mode should provide ~20% mean Strehl diffraction-limited images over the band 0.5-1.0 \\{mu}m. In IFS mode, the VisAO instrument will provide R~1,800 spectra over the band 0.6-1.05 \\{mu}m. Our unprecedented 20 mas spatially resolved visible spectra would be the highest spatial resolution achieved to date, either from the ground or in space. We also present lab results from our recently fabricated...

  13. 1H MAS NMR characterization of hydrogen over silica-supported rhodium catalyst

    2001-01-01

    Hydrogen species in both SiO2 and Rh/SiO2 catalysts pretreated indifferent atmospheres (H2, O2, helium or air) at different temperatures (773 or 973 K) were investigated by means of 1H MAS NMR. In SiO2 and O2-pretreated catalysts, a series of downfield signals at ~7.0, 3.8-4.0, 2.0 and 1.5-1.0 were detected. The first two signals can be attributed to strongly adsorbed and physisorbed water and the others to terminal silanol (SiOH) and SiOH under the screening of oxygen vacancies in SiO2 lattice, respectively. Besides the above signals, both upfield signal at ~-110 and downfield signals at 3.0 and 0.0 were also detected in H2-pretreated catalyst, respectively. The upfield signal at ~-110 originated from the dissociative adsorption of H2 over rhodium and was found to consist of both the contributions of reversible and irreversible hydrogen. There also probably existed another dissociatively adsorbed hydrogen over rhodium, which was known to be b hydrogen and in a unique form of "delocalized hydrogen". It was presumed that the b hydrogen had an upfield shift of ca. -20- -50, though its 1H NMR signals, which, having been masked by the spinning sidebands of Si-OH, failed to be directly detected out. The downfield signal at 3.0 was assigned to spillover hydrogen weakly bound by the bridge oxygen of SiO2. Another downfield signal at 0.0 was assigned to hydrogen held in the oxygen vacancies of SiO2 (Si-H species), suffering from the screening of trapped electrons. Both the spillover hydrogen and the Si-H resulted from the migration of the reversible hydrogen and the b hydrogen from rhodium to SiO2 in the close vicinity. It was proved that the above migration of hydrogen was preferred to occur at higher temperature than at lower temperature.

  14. Promising Cornelian Cherry (Cornus mas L. Genotypes from Natural Population in Serbia

    Sandra Bijelić

    2012-03-01

    Full Text Available Serbia is a rich in Cornelian cherry (Cornus mas L. populations that grow naturally in a variety of locations. At Faculty of Agriculture in Novi Sad, an intensive breeding program on this fruit species started in 2006. The paper reviews morphometric characteristics of 16 Cornelian cherry genotypes collected on the territory of the Vojvodina Province and Mačva region in 2008 and 2009, which were found to be superior in terms of fruit size and quality. On average for the two years, the genotype PPC1 had the highest fruit length (27.79 mm, fruit width (18.97 mm, fruit weight (6.61 g, mesocarp weight (5.86 g and the flesh to stone ratio (88.46%. The genotype Žuti had the longest fruit. The effect of year of growing was statistically significant for all studied traits, except for fruit width and fruit shape index. Fruit weight, economically the most important characteristic, ranged in the interval from 2.25 to 6.61 g, with a large number of genotypes having the fruit weight over 3 g. All of the tested genotypes had satisfactory flesh to stone ratio, from 79.00% (CPC9 to 88.46% (PPC1. The highest variability was detected for mesocarp weight (36.48% and fruit weight (33.19% in 2009, while flesh to stone ratio was the most stable characteristic in both test years (CV = 3.84 and 4.09% in 2008 and 2009, respectively. The genotype SKC had highest contents of soluble matter (32.37%, total sugars (25.38% and reducing sugars (23.67%. Fruits of the genotype KC2 had highest contents of vitamin C (39.22 mg/100 g fruit and total acids (3.60%. Selection Apatinski rani had the highest content of anthocyanins (109.36 mg/100 g fruit. Depending on the genotype, protein content ranged from 1.43 to 2.71%, and tannin content from 0.65% to 1.31%.

  15. MAS-NMR study of lithium zinc silicate glasses and glass-ceramics with various ZnO content

    Lithium zinc silicate glasses of composition (mol%): 17.5Li2O-(72-x)SiO2-xZnO-5.1Na2O-1.3P2O5-4.1B2O3, 5.5≤x≤17.7, were prepared by conventional melt-quenched technique and converted to glass-ceramic by controlled crystallization process. 29Si and 31P MAS-NMR was used to characterize the structure of both glass and glass-ceramic samples. Despite the complex glass composition, Q2, Q3 and Q4 sites are identified from 29Si MAS-NMR, which relative intensities are found to vary with the ZnO content, indicating a network depolymerization by ZnO. Moreover, well separated Q3 and Q4 resonances for low ZnO content indicates the occurrence of phase separation. From 31P MAS-NMR, it is seen that phosphorus is mainly present in the form of ortho-(Q0) and pyro-phosphate (Q1) structural units and variation of ZnO content did not have much effect on these resonances, which provides an additional evidence for phase separation in the glass. On conversion to glass-ceramics, lithium disilicate (Li2Si2O5), lithium zinc ortho-silicate (Li3Zn0.5SiO4), tridymite (SiO2) and cristobalite (SiO2) were identified as major silicate crystalline phases. Using 29Si MAS-NMR, quantification of these silicate crystalline phases is carried out and correlated with the ZnO content in the glass-ceramics samples. In addition, 31P spectra unambiguously revealed the presence of crystalline Li3PO4 and (Na,Li)3PO4 in the glass-ceramics. - Graphical abstract: 29Si and 31P MAS-NMR analyses were carried out on multi-component Li2O-SiO2-ZnO-Na2O-P2O5-B2O3 glasses and glass-ceramics developed for sealing application. Structural data are reported, including phase separation process and quantification of amorphous and crystalline phases

  16. Promising Cornelian Cherry (Cornus mas L. Genotypes from Natural Population in Serbia

    Sandra Bijelic

    2014-02-01

    Full Text Available Normal 0 21 false false false SR X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Serbia is a rich in Cornelian cherry (Cornus mas L. populations that grow naturally in a variety of locations. At Faculty of Agriculture in Novi Sad, an intensive breeding program on this fruit species started in 2006. The paper reviews morphometric characteristics of 16 Cornelian cherry genotypes collected on the territory of the Vojvodina Province and Mačva region in 2008 and 2009, which were found to be superior in terms of fruit size and quality. On average for the two years, the genotype PPC1 had the highest fruit length (27.79 mm, fruit width (18.97 mm, fruit weight (6.61 g, mesocarp weight (5.86 g and the flesh to stone ratio (88.46%. The genotype Žuti had the longest fruit. The effect of year of growing was statistically significant for all studied traits, except for fruit width and fruit shape index. Fruit weight, economically the most important characteristic, ranged in the interval from 2.25 to 6.61 g, with a large number of genotypes having the fruit weight over 3 g. All of the tested genotypes had satisfactory flesh to stone ratio, from 79.00% (CPC9 to 88.46% (PPC1. The highest variability was detected for mesocarp weight (36.48% and fruit weight (33.19% in 2009, while flesh to stone ratio was

  17. Determination of the structural changes by Raman and 13C CP/MAS NMR spectroscopy on native corn starch with plasticizers

    The plasticizing - antiplasticizing effect of water and glycerol contents on native corn starch samples is investigated by FT-Raman and 13C CP/MAS NMR spectroscopy. The presence of both amorphous and crystalline structural phases was evidenced in pure native corn starch and also in the samples containing plasticizers. Among the crystalline starch structures, the A- and V- types were suggested by CP/MAS NMR spectra

  18. Determination of the structural changes by Raman and {sup 13}C CP/MAS NMR spectroscopy on native corn starch with plasticizers

    Cozar, O. [Academy of Romanian Scientists, Splaiul Independentei 54, 050094, Bucharest, Romania and National Institute of Research-Development for Machines and Installations Designed to Agriculture and Food Industry - INMA Bucureşti - Cluj-Napoca Branch (Romania); Filip, C.; Tripon, C. [National Institute for Research and Development of Isotopic and Molecular Technologies, 65-103 Donath, 400293 Cluj-Napoca (Romania); Cioica, N.; Coţa, C.; Nagy, E. M. [National Institute of Research-Development for Machines and Installations Designed to Agriculture and Food Industry - INMA Bucureşti - Cluj-Napoca Branch, RO-400458 Cluj-Napoca (Romania)

    2013-11-13

    The plasticizing - antiplasticizing effect of water and glycerol contents on native corn starch samples is investigated by FT-Raman and {sup 13}C CP/MAS NMR spectroscopy. The presence of both amorphous and crystalline structural phases was evidenced in pure native corn starch and also in the samples containing plasticizers. Among the crystalline starch structures, the A- and V- types were suggested by CP/MAS NMR spectra.

  19. Angiotensin-converting enzyme 2/angiotensin-(1-7)/Mas axis activates Akt signaling to ameliorate hepatic steatosis.

    Cao, Xi; Yang, Fangyuan; Shi, Tingting; Yuan, Mingxia; Xin, Zhong; Xie, Rongrong; Li, Sen; Li, Hongbing; Yang, Jin-Kui

    2016-01-01

    The classical axis of renin-angiotensin system (RAS), angiotensin (Ang)-converting enzyme (ACE)/Ang II/AT1, contributes to the development of non-alcoholic fatty liver disease (NAFLD). However, the role of bypass axis of RAS (Angiotensin-converting enzyme 2 (ACE2)/Ang-(1-7)/Mas) in hepatic steatosis is still unclear. Here we showed that deletion of ACE2 aggravates liver steatosis, which is correlated with the increased expression of hepatic lipogenic genes and the decreased expression of fatty acid oxidation-related genes in the liver of ACE2 knockout (ACE2(-/y)) mice. Meanwhile, oxidative stress and inflammation were also aggravated in ACE2(-/y) mice. On the contrary, overexpression of ACE2 improved fatty liver in db/db mice, and the mRNA levels of fatty acid oxidation-related genes were up-regulated. In vitro, Ang-(1-7)/ACE2 ameliorated hepatic steatosis, oxidative stress and inflammation in free fatty acid (FFA)-induced HepG2 cells, and what's more, Akt inhibitors reduced ACE2-mediated lipid metabolism. Furthermore, ACE2-mediated Akt activation could be attenuated by blockade of ATP/P2 receptor/Calmodulin (CaM) pathway. These results indicated that Ang-(1-7)/ACE2/Mas axis may reduce liver lipid accumulation partly by regulating lipid-metabolizing genes through ATP/P2 receptor/CaM signaling pathway. Our findings support the potential role of ACE2/Ang-(1-7)/Mas axis in prevention and treatment of hepatic lipid metabolism. PMID:26883384

  20. The Magellan Adaptive Secondary VisAO Camera: diffraction-limited broadband visible imaging and 20mas fiber array IFU

    Kopon, Derek; Close, Laird M.; Males, Jared; Gasho, Victor; Follette, Katherine

    2010-07-01

    The Magellan Adaptive Secondary AO system, scheduled for first light in the fall of 2011, will be able to simultaneously perform diffraction limited AO science in both the mid-IR, using the BLINC/MIRAC4 10μm camera, and in the visible using our novel VisAO camera. The VisAO camera will be able to operate as either an imager, using a CCD47 with 8.5 mas pixels, or as an IFS, using a custom fiber array at the focal plane with 20 mas elements in its highest resolution mode. In imaging mode, the VisAO camera will have a full suite of filters, coronagraphic focal plane occulting spots, and SDI prism/filters. The imaging mode should provide ~20% mean Strehl diffraction-limited images over the band 0.5-1.0 μm. In IFS mode, the VisAO instrument will provide R~1,800 spectra over the band 0.6-1.05 μm. Our unprecedented 20 mas spatially resolved visible spectra would be the highest spatial resolution achieved to date, either from the ground or in space. We also present lab results from our recently fabricated advanced triplet Atmospheric Dispersion Corrector (ADC) and the design of our novel wide-field acquisition and active optics lens. The advanced ADC is designed to perform 58% better than conventional doublet ADCs and is one of the enabling technologies that will allow us to achieve broadband (0.5-1.0μm) diffraction limited imaging and wavefront sensing in the visible.

  1. Radioprotective properties of the phytochemically characterized extracts of Crataegus monogyna, Cornus mas and Gentianella austriaca on human lymphocytes in vitro.

    Leskovac, Andreja; Joksic, Gordana; Jankovic, Teodora; Savikin, Katarina; Menkovic, Nebojsa

    2007-09-01

    The objective of the present study is to evaluate the radioprotective properties of extracts of Crataegus monogyna Jacq. (Rosaceae) fruit, Cornus mas L. (Cornaceae) leaves and Gentianella austriaca (A. Kern. & Jos. Kern.) Holub (Gentianaceae) aerial parts on cultured human peripheral blood lymphocytes in vitro after irradiation with 2 Gy of 60Co gamma-rays. Plants were collected at Mt. Maljen in Serbia, air-dried and powdered, and the total phenolic content was analyzed. In C. mas leaves, ellagic and gallic acid were found to be the dominant compounds, whereas C. monogyna fruit was rich in procyanidins and flavonoids. The main constituents of G. austriaca aerial parts were gamma-pyrones and secoiridoids. Cell cultures were treated with four different doses of plant extracts (0.1 mg/mL to 0.4 mg/mL). Treatment with the lowest dose gave the best protective effect. Significant radiorecovery potentials of C. mas and C. monogyna were observed, seen as a reduced incidence of radiation-induced micronuclei (19.23% and 13.24%, respectively), reduced levels of lipid peroxidation products (50.04% and 13.18%, respectively) and two-fold enhanced apoptosis. Both extracts slowed down cell proliferation gradually, enabling more time for repair. G. austriaca possesses strong antioxidant properties, significantly reducing lipid peroxidation and the incidence of micronuclei (for 30.88% and 35.56%, respectively) while enhancing apoptosis with no perturbation of the cell cycle. This study may contribute to the search for novel radioprotective agents. PMID:17764067

  2. Evaluación ecotoxicológica del FitoMas-H en lombriz de tierra y abejas

    Axel Mancebo; Ramón Montano; Consuelo González; Yana González; Bárbara González; Ana M. Bada

    2008-01-01

    Aunque constituye una necesidad el aumento de las producciones agrícolas, y actualmente se estimula la disminución del uso de productos químicos, mediante su sustitución por fertilizantes orgánicos y biológicos, es preciso evaluar el impacto de éstos sobre las especies del ecosistema en el cual se liberan. Con el objetivo de realizar la evaluación ecotoxicológica en sistemas terrestres del FitoMas-H, se realizaron los ensayos de toxicidad aguda en lombriz de tierra (Eisenia foetida) y de toxi...

  3. Protective effects of vitamin E and Cornus mas fruit extract on methotrexate-induced cytotoxicity in sperms of adult mice

    Zarei, Leila; Sadrkhanlou, Rajabali; Shahrooz, Rasoul; Malekinejad, Hassan; Eilkhanizadeh, Behroz; Ahmadi, Abbas

    2014-01-01

    This study was aimed to assess the protective effects of Cornus mas fruit extract (CMFE) and vitamin E (Vit E) on sperm quality parameters in the methotrexate (MTX)-treated mice. Forty-eight young adult male mice (8-12 weeks) were randomly divided into six groups including control and test groups. The control group received normal saline orally , and the test groups were treated MTX (20 mg kg-1, ip, once weekly), MTX + CMFE (250 mg kg-1), MTX + CMFE (500 mg kg-1), MTX + CMFE (1000 mg kg-1), a...

  4. CaCl2-Accelerated Hydration of Tricalcium Silicate: A STXM Study Combined with 29Si MAS NMR

    Qinfei Li; Yong Ge; Guoqing Geng; Sungchul Bae; Monteiro, Paulo J. M.

    2015-01-01

    The effect of calcium chloride (CaCl2) on tricalcium silicate (C3S) hydration was investigated by scanning transmission X-ray microscopy (STXM) with Near Edge X-ray Absorption Fine Structure (NEXAFS) spectra and 29Si MAS NMR. STXM is demonstrated to be a powerful tool for studying the chemical composition of a cement-based hydration system. The Ca L3,2-edge NEXAFS spectra obtained by examining C3S hydration in the presence of CaCl2 showed that this accelerator does not change the coordination...

  5. 27Al Magic Angle Spinning–Nuclear Magnetic Resonance (MAS-NMR) Analyses Applied to Historical Mortars

    Hanzlíček, Tomáš; Perná, Ivana; Brus, Jiří

    2013-01-01

    Roč. 7, č. 2 (2013), s. 153-164. ISSN 1558-3058 R&D Projects: GA AV ČR IAA300460702 Institutional research plan: CEZ:AV0Z30460519; CEZ:AV0Z40500505 Keywords : mortars * magic angle spinning –nuclear magnetic resonance (MAS-NMR) in solid state * alumina-silicates Subject RIV: DM - Solid Waste and Recycling Impact factor: 0.714, year: 2013 http://www.tandfonline.com/doi/abs/10.1080/15583058.2011.624253

  6. Microscopic structural analysis of fractured silk fibers from Bombyx mori and Samia cynthia ricini using 13C CP/MAS NMR with a 1 mm microcoil MAS NMR probehead

    Yamauchi, Kazuo

    2010-07-01

    Conformational changes have been studied in silk fibers from the domestic silkworm Bombyx mori and a wild silkworm Samia cynthia ricini as a result of fractured by stretching. About 300 samples consisting of only the fractured regions of [1-13C]Ala or [1-13C]Gly labeled silk fibers were collected and observed by 13C CP/MAS NMR spectra. The total amount of these fractured fibers is only about 1 mg and therefore we used a home-built 1 mm microcoil MAS NMR probehead. A very small increase in the fraction of random coil was noted for the alanine regions of both silk fibroins and for the glycine region of B. mori silk fibroin. However, there is no difference in the spectra before and after fractured for the glycine region of S. c. ricini silk fibroin. Thus, the influence of fracture occurs exclusively at the Ala region for S. c. ricini. The relationship between sequence, fracture and structure is discussed. © 2010 Elsevier Inc. All rights reserved.

  7. Sensitivity Gains, Linearity, and Spectral Reproducibility in Nonuniformly Sampled Multidimensional MAS NMR Spectra of High Dynamic Range.

    Suiter, Christopher L.; Paramasivam, Sivakumar; Hou, Guangjin; Sun, Shangjin; Rice, David M.; Hoch, Jeffrey C.; Rovnyak, David S.; Polenova, Tatyana E.

    2014-04-22

    Recently, we have demonstrated that considerable inherent sensitivity gains are attained in MAS NMR spectra acquired by nonuniform sampling (NUS) and introduced maximum entropy interpolation (MINT) processing that assures the linearity of transformation between the time and frequency domains. In this report, we examine the utility of the NUS/MINT approach in multidimensional datasets possessing high dynamic range, such as homonuclear 13C–13C correlation spectra. We demonstrate on model compounds and on 1–73-(U-13C,15N)/74–108-(U-15N) E. coli thioredoxin reassembly, that with appropriately constructed 50 % NUS schedules inherent sensitivity gains of 1.7–2.1-fold are readily reached in such datasets. We show that both linearity and line width are retained under these experimental conditions throughout the entire dynamic range of the signals. Furthermore, we demonstrate that the reproducibility of the peak intensities is excellent in the NUS/MINT approach when experiments are repeated multiple times and identical experimental and processing conditions are employed. Finally, we discuss the principles for design and implementation of random exponentially biased NUS sampling schedules for homonuclear 13C–13C MAS correlation experiments that yield high quality artifact-free datasets.

  8. Asynchronous through-bond homonuclear isotropic mixing: application to carbon–carbon transfer in perdeuterated proteins under MAS

    Kulminskaya, Natalia; Vasa, Suresh Kumar; Giller, Karin; Becker, Stefan; Linser, Rasmus, E-mail: rali@nmr.mpibpc.mpg.de [Max Planck Institute for Biophysical Chemistry, Department of NMR-based Structural Biology (Germany)

    2015-11-15

    Multiple-bond carbon–carbon homonuclear mixing is a hurdle in extensively deuterated proteins and under fast MAS due to the absence of an effective proton dipolar-coupling network. Such conditions are now commonly employed in solid-state NMR spectroscopy. Here, we introduce an isotropic homonuclear {sup 13}C–{sup 13}C through-bond mixing sequence, MOCCA, for the solid state. Even though applied under MAS, this scheme performs without rotor synchronization and thus does not pose the usual hurdles in terms of power dissipation for fast spinning. We compare its performance with existing homonuclear {sup 13}C–{sup 13}C mixing schemes using a perdeuterated and partially proton-backexchanged protein. Based on the analysis of side chain carbon–carbon correlations, we show that particularly MOCCA with standard 180-degree pulses and delays leading to non-rotor-synchronized spacing performs exceptionally well. This method provides high magnetization transfer efficiency for multiple-bond transfer in the aliphatic region compared with other tested mixing sequences. In addition, we show that this sequence can also be tailor-made for recoupling within a selected spectral region using band-selective pulses.

  9. Site-resolved (2)H relaxation experiments in solid materials by global line-shape analysis of MAS NMR spectra.

    Lindh, E L; Stilbs, P; Furó, I

    2016-07-01

    We investigate a way one can achieve good spectral resolution in (2)H MAS NMR experiments. The goal is to be able to distinguish between and study sites in various deuterated materials with small chemical shift dispersion. We show that the (2)H MAS NMR spectra recorded during a spin-relaxation experiment are amenable to spectral decomposition because of the different evolution of spectral components during the relaxation delay. We verify that the results are robust by global least-square fitting of the spectral series both under the assumption of specific line shapes and without such assumptions (COmponent-REsolved spectroscopy, CORE). In addition, we investigate the reliability of the developed protocol by analyzing spectra simulated with different combinations of spectral parameters. The performance is demonstrated in a model material of deuterated poly(methacrylic acid) that contains two (2)H spin populations with similar chemical shifts but different quadrupole splittings. In (2)H-exchanged cellulose containing two (2)H spin populations with very similar chemical shifts and quadrupole splittings, the method provides new site-selective information about the molecular dynamics. PMID:27152833

  10. 1H HR-MAS NMR Spectroscopy and the Metabolite Determination of Typical Foods in Mediterranean Diet

    Carmelo Corsaro

    2015-01-01

    Full Text Available NMR spectroscopy has become an experimental technique widely used in food science. The experimental procedures that allow precise and quantitative analysis on different foods are relatively simple. For a better sensitivity and resolution, NMR spectroscopy is usually applied to liquid sample by means of extraction procedures that can be addressed to the observation of particular compounds. For the study of semisolid systems such as intact tissues, High-Resolution Magic Angle Spinning (HR-MAS has received great attention within the biomedical area and beyond. Metabolic profiling and metabolism changes can be investigated both in animal organs and in foods. In this work we present a proton HR-MAS NMR study on the typical vegetable foods of Mediterranean diet such as the Protected Geographical Indication (PGI cherry tomato of Pachino, the PGI Interdonato lemon of Messina, several Protected Designation of Origin (PDO extra virgin olive oils from Sicily, and the Traditional Italian Food Product (PAT red garlic of Nubia. We were able to identify and quantify the main metabolites within the studied systems that can be used for their characterization and authentication.

  11. Surface Characterization of Some Novel Bonded Phase Packing Materials for HPLC Columns Using MAS-NMR Spectroscopy

    Jude Abia

    2015-03-01

    Full Text Available Information on the surface properties of three novel chemically bonded phase packing materials for High performance liquid chromatography (HPLC were obtained using spectra obtained by solid state cross-polarization (CP magic-angle spinning (MAS nuclear magnetic resonance (NMR spectroscopic experiments for the 29Si, and 13C nuclei. These packing materials were: Cogent bidentate C18 bonded to type-C silica, hybrid packing materials XTerra MS C18, and XBridge Prep. C18. The spectra obtained using cross-polarization magic angle spinning (CP-MAS on the Cogent bidentate C18 bonded to type-C silica show the surface to be densely populated with hydride groups (Si-H, with a relative surface coverage exceeding 80%. The hybrid packing materials XTerra and XBridge gave spectra that reveal the silicon atoms to be bonded to organic moieties embedded in the molecular structure of these materials with over 90% of the alkyl silicon atoms found within the completely condensed silicon environments. The hydrolytic stability of these materials were investigated in acidic aqueous solutions at pHs of 7.0 and 3.0, and it was found that while the samples of XTerra and XBridge were not affected by hydrolysis at this pH range, the sample of Cogent lost a significant proportion of its Si-H groups after five days of treatment in acidic aqueous solution.

  12. Asynchronous through-bond homonuclear isotropic mixing: application to carbon–carbon transfer in perdeuterated proteins under MAS

    Multiple-bond carbon–carbon homonuclear mixing is a hurdle in extensively deuterated proteins and under fast MAS due to the absence of an effective proton dipolar-coupling network. Such conditions are now commonly employed in solid-state NMR spectroscopy. Here, we introduce an isotropic homonuclear 13C–13C through-bond mixing sequence, MOCCA, for the solid state. Even though applied under MAS, this scheme performs without rotor synchronization and thus does not pose the usual hurdles in terms of power dissipation for fast spinning. We compare its performance with existing homonuclear 13C–13C mixing schemes using a perdeuterated and partially proton-backexchanged protein. Based on the analysis of side chain carbon–carbon correlations, we show that particularly MOCCA with standard 180-degree pulses and delays leading to non-rotor-synchronized spacing performs exceptionally well. This method provides high magnetization transfer efficiency for multiple-bond transfer in the aliphatic region compared with other tested mixing sequences. In addition, we show that this sequence can also be tailor-made for recoupling within a selected spectral region using band-selective pulses

  13. Combined zero-quantum and spin-diffusion mixing for efficient homonuclear correlation spectroscopy under fast MAS: broadband recoupling and detection of long-range correlations

    Lu, Xingyu, E-mail: luxingyu@udel.edu; Guo, Changmiao, E-mail: cmguo@udel.edu; Hou, Guangjin, E-mail: hou@udel.edu; Polenova, Tatyana, E-mail: tpolenov@udel.edu [University of Delaware, Department of Chemistry and Biochemistry (United States)

    2015-01-15

    Fast magic angle spinning (MAS) NMR spectroscopy is emerging as an essential analytical and structural biology technique. Large resolution and sensitivity enhancements observed under fast MAS conditions enable structural and dynamics analysis of challenging systems, such as large macromolecular assemblies and isotopically dilute samples, using only a fraction of material required for conventional experiments. Homonuclear dipolar-based correlation spectroscopy constitutes a centerpiece in the MAS NMR methodological toolbox, and is used essentially in every biological and organic system for deriving resonance assignments and distance restraints information necessary for structural analysis. Under fast MAS conditions (rotation frequencies above 35–40 kHz), dipolar-based techniques that yield multi-bond correlations and non-trivial distance information are ineffective and suffer from low polarization transfer efficiency. To overcome this limitation, we have developed a family of experiments, CORD–RFDR. These experiments exploit the advantages of both zero-quantum RFDR and spin-diffusion based CORD methods, and exhibit highly efficient and broadband dipolar recoupling across the entire spectrum, for both short-range and long-range correlations. We have verified the performance of the CORD–RFDR sequences experimentally on a U-{sup 13}C,{sup 15}N-MLF tripeptide and by numerical simulations. We demonstrate applications of 2D CORD–RFDR correlation spectroscopy in dynein light chain LC8 and HIV-1 CA tubular assemblies. In the CORD–RFDR spectra of LC8 acquired at the MAS frequency of 40 kHz, many new intra- and inter-residue correlations are detected, which were not observed with conventional dipolar recoupling sequences. At a moderate MAS frequency of 14 kHz, the CORD–RFDR experiment exhibits excellent performance as well, as demonstrated in the HIV-1 CA tubular assemblies. Taken together, the results indicate that CORD–RFDR experiment is beneficial in a

  14. SCAM-STMAS: satellite-transition MAS NMR of quadrupolar nuclei with self-compensation for magic-angle misset

    Ashbrook, Sharon E.; Wimperis, Stephen

    2003-06-01

    Several methods are available for the acquisition of high-resolution solid-state NMR spectra of quadrupolar nuclei with half-integer spin quantum number. Satellite-transition MAS (STMAS) offers an approach that employs only conventional MAS hardware and can yield substantial signal enhancements over the widely used multiple-quantum MAS (MQMAS) experiment. However, the presence of the first-order quadrupolar interaction in the satellite transitions imposes the requirement of a high degree of accuracy in the setting of the magic angle on the NMR probehead. The first-order quadrupolar interaction is only fully removed if the sample spinning angle, χ, equals cos-1(1/ 3) exactly and rotor synchronization is performed. The required level of accuracy is difficult to achieve experimentally, particularly when the quadrupolar interaction is large. If the magic angle is not set correctly, the first-order splitting is reintroduced and the spectral resolution is severely compromised. Recently, we have demonstrated a novel STMAS method (SCAM-STMAS) that is self-compensated for angle missets of up to ±1° via coherence transfer between the two different satellite transitions ST +( mI=+3/2↔+1/2) and ST -( mI=-1/2↔-3/2) midway through the t1 period. In this work we describe in more detail the implementation of SCAM-STMAS and demonstrate its wider utility through 23Na ( I=3/2), 87Rb ( I=3/2), 27Al ( I=5/2), and 59Co ( I=7/2) NMR. We discuss linewidths in SCAM-STMAS and the limits over which angle-misset compensation is achieved and we demonstrate that SCAM-STMAS is more tolerant of temporary spinning rate fluctuations than STMAS, resulting in less " t1 noise" in the two-dimensional spectrum. In addition, alternative correlation experiments, for example involving the use of double-quantum coherences, that similarly display self-compensation for angle misset are investigated. The use of SCAM-STMAS is also considered in systems where other high-order interactions, such as third

  15. Determination of nuclear distances and chemical-shift anisotropy from 1H MAS NMR sideband patterns of surface OH groups

    Fenzke, Dieter; Hunger, Michael; Pfeifer, Harry

    A procedure is described which allows a separate determination of the proton-aluminum distance and of the chemical-shift anisotropy for the bridging OH groups of crystalline molecular sieves from their 'H MAS NMR sideband patterns. For the bridging OH groups which point into the 6-rings of the framework (line "c"), the 1H- 27Al distance could be determined to be 0.237 ± 0.004 and 0.234 ± 0.004 nm for molecular sieves of type H-Y and SAPO-5, respectively. In contrast, for the bridging OH groups of the 12-rings (line "b"), the corresponding distances are equal and distinctly larger, 0.248 ± 0.004 nm. Within the limits of error, the values of the chemical-shift anisotropy are equal (about 19 ± 2 ppm) except for line b of SAPO-5, which exhibits a much smaller value of 14.5 ± 2 ppm.

  16. Indication of Erosion At Tea - Plantation - Gunung Mas - Puncak - West Java Using Environmental Isotop of 137Cs

    Erosion is a natural phenomena as a result of deforestation. Indication of erosion could be investigated by nuclear method that is used a natural radioisotope of 137Cs in the soil. The present of 137Cs in environment is a result of the nuclear weapon testing in the middle of 1950's. The result of this pre study showed that the total activity of 137Cs in the soil of Tea - Plantation - Gunung Mas - Puncak - West Java is between 150 Bq/m2-1872 Bq/m2. The result showed that the activity of 137Cs could be detected, and the erosion or deposition could be seen at the points of the investigation

  17. Schémas de type Godunov pour la modélisation hydrodynamique et magnétohydrodynamique

    Vides Higueros, Jeaniffer

    2014-01-01

    L’objectif principal de cette thèse concerne l’étude, la conception et la mise en œuvre numérique de schémas volumes finis associés aux solveurs de type Godunov. On s’intéresse à des systèmes hyperboliques de lois de conservation non linéaires, avec une attention particulière sur les équations d’Euler et les équations MHD idéale. Tout d’abord, nous dérivons un solveur de Riemann simple et véritablement multidimensionnelle, pouvant s’appliquer à tout système de lois de conservation. Ce solveur...

  18. 13C MAS NMR studies of the effects of hydration on the cell walls of potatoes and Chinese water chestnuts.

    Tang, H; Belton, P S; Ng, A; Ryden, P

    1999-02-01

    13C NMR with magic angle spinning (MAS) has been employed to investigate the cell walls of potatoes and Chinese water chestnuts over a range of hydration levels. Both single-pulse excitation (SPEMAS) and cross-polarization (CPMAS) experiments were carried out. Hydration led to a substantial increase in signal intensities of galactan and galacturonan in the SPEMAS spectra and a decrease in line width, implying mobilization in the backbone and side chains of pectin. In CPMAS spectra of both samples, noncellulose components showed signal loss as hydration increased. However, the signals of some galacturonan in the 3(1) helix configuration remained in the spectra even when the water content was as high as 110%. Cellulose was unaffected. It is concluded that the pectic polysaccharides experience a distribution of molecular conformations and mobility, whereas cellulose remained as typical rigid solid. PMID:10563925

  19. Residual methyl protonation in perdeuterated proteins for multi-dimensional correlation experiments in MAS solid-state NMR spectroscopy

    Agarwal, Vipin; Reif, Bernd

    2008-09-01

    NMR studies involving perdeuterated proteins focus in general on exchangeable amide protons. However, non-exchangeable sites contain as well a small amount of protons as the employed precursors for protein biosynthesis are not completely proton depleted. The degree of methyl group protonation is in the order of 9% for CD 2H using >97% deuterium enriched glucose. We show in this manuscript that this small amount of residual protonation is sufficient to perform 2D and 3D MAS solid-state NMR experiments. In particular, we suggest a HCCH-TOBSY type experiment which we successfully employ to assign the methyl resonances in aliphatic side chains in a perdeuterated sample of the SH3 domain of chicken α-spectrin.

  20. Assignment of amide proton signals by combined evaluation of HN, NN and HNCA MAS-NMR correlation spectra

    Rossum, Barth-Jan van; Castellani, Federica [Forschungsinstitut fuer Molekulare Pharmakologie (FMP) (Germany); Pauli, Jutta [BAM (Germany); Rehbein, Kristina [Forschungsinstitut fuer Molekulare Pharmakologie (FMP) (Germany); Hollander, J.; Groot, Huub J.M. de [BAM (Germany); Oschkinat, Hartmut [Forschungsinstitut fuer Molekulare Pharmakologie (FMP) (Germany)], E-mail: Oschkinat@fmp-berlin.de

    2003-03-15

    In this paper, we present a strategy for the {sup 1}H{sup N} resonance assignment in solid-state magic-angle spinning (MAS) NMR, using the {alpha}-spectrin SH3 domain as an example. A novel 3D triple resonance experiment is presented that yields intraresidue H{sup N}-N-C{sup {alpha}} correlations, which was essential for the proton assignment. For the observable residues, 52 out of the 54 amide proton resonances were assigned from 2D ({sup 1}H-{sup 15}N) and 3D ({sup 1}H-{sup 15}N-{sup 13}C) heteronuclear correlation spectra. It is demonstrated that proton-driven spin diffusion (PDSD) experiments recorded with long mixing times (4 s) are helpful for confirming the assignment of the protein backbone {sup 15}N resonances and as an aid in the amide proton assignment.

  1. L'installation de methanisation de Mas El Cros. Evaluation de 16 années de fonctionement

    Flotats Ripoll, Xavier

    2000-01-01

    L’installation de méthanisation de l’élevage porcin du ‘’Mas El cros’’ (La Garrotxa, Catalogne, Espagne) a été conçue en 1981, en même temps que l’exploitation. Ceci a permis d’intégrer l’installation, sa conduite et sa maintenance dans la conception générale de l’exploitation. L’installation a été mise end route fin 1983. Le système de chauffage des bâtiments a été mis en route en 1984. En 1994, il a été arrêté pour être remplacé, à cause de problèmes de corrosion. Entre 1984 et décembre...

  2. CONCEPTUALIZACIÓN Y ANÁLISIS DE UN SISTEMA MULTI-AGENTE PEDAGÓGICO UTILIZANDO LA METODOLOGÍA MAS-COMMONKADS

    JOVANI ALBERTO JIMÉNEZ BUILES; DEMETRIO ARTURO OVALLE CARRANZA; JOHN WILLIAM BRANCH BEDOYA

    2009-01-01

    En este artículo se presentan la conceptualización y el análisis de un sistema multi-agente (MAS) pedagógico utilizando la metodología MAS-CommonKADS; la cual es una de las más reconocidaspara el modelado de MAS. El propósito de la fase de conceptualización es comprender el problema, identificar los actores activos y pasivo, además de elaborar los requisitos que permitan formular unos objetivos para generar la solución. En la fase de análisis se definen los modelos de agente, tareas, coordina...

  3. Conceptualización y análisis de un sistema multi-agente pedagógico utilizando la metodología mas-commonkads

    JIMÉNEZ BUILES, JOVANI ALBERTO; Ovalle Carranza, Demetrio Arturo; BRANCH BEDOYA, JOHN WILLIAM

    2009-01-01

    En este artículo se presentan la conceptualización y el análisis de un sistema multi-agente (MAS) pedagógico utilizando la metodología MAS-CommonKADS; la cual es una de las más reconocidas para el modelado de MAS. El propósito de la fase de conceptualización es comprender el problema, identificar los actores activos y pasivo, además de elaborar los requisitos que permitan formular unos objetivos para generar la solución. En la fase de análisis se definen los modelos de agente, tareas, coordin...

  4. CONCEPTUALIZACIÓN Y ANÁLISIS DE UN SISTEMA MULTI-AGENTE PEDAGÓGICO UTILIZANDO LA METODOLOGÍA MAS-COMMONKADS

    JOVANI ALBERTO JIMÉNEZ BUILES; DEMETRIO ARTURO OVALLE CARRANZA; JOHN WILLIAM BRANCH BEDOYA

    2009-01-01

    En este artículo se presentan la conceptualización y el análisis de un sistema multi-agente (MAS) pedagógico utilizando la metodología MAS-CommonKADS; la cual es una de las más reconocidas para el modelado de MAS. El propósito de la fase de conceptualización es comprender el problema, identificar los actores activos y pasivo, además de elaborar los requisitos que permitan formular unos objetivos para generar la solución. En la fase de análisis se definen los modelos de agente, tareas, coordin...

  5. Nanoscopic yttrium oxide fluorides: non-aqueous fluorolytic sol-gel synthesis and structural insights by 19F and 89Y MAS NMR.

    Scholz, G; Dreger, M; Bertram, R; Kemnitz, E

    2015-08-14

    Nanoscopic yttrium acetate fluorides Y(CH(3)COO)(3-z)F(z) and yttrium oxide fluorides YO(3-z)/(2)F(z )were prepared with tunable Y/F molar ratios via the fluorolytic sol-gel route. All samples were characterized by X-ray diffraction, elemental analysis and thermal analysis. In addition, local structures of all samples were studied by (19)F MAS, (19)F-(89)Y CP MAS and (1)H-(89)Y CP MAS NMR spectroscopy and the respective chemical shifts are given. For both classes of compounds, only the fluorination using one equivalent of F (z = 1) leads to defined, well crystalline matrices: yttrium acetate fluoride Y(CH(3)COO)(2)F and r-YOF. PMID:26133504

  6. Application of (119)Sn CPMG MAS NMR for Fast Characterization of Sn Sites in Zeolites with Natural (119)Sn Isotope Abundance.

    Kolyagin, Yury G; Yakimov, Alexander V; Tolborg, Søren; Vennestrøm, Peter N R; Ivanova, Irina I

    2016-04-01

    (119)Sn CPMG MAS NMR is demonstrated to be a fast and efficient method for characterization of Sn-sites in Sn-containing zeolites. Tuning of the CPMG echo-train sequence decreases the experimental time by a factor of 5-40 in the case of as-synthesized and hydrated Sn-BEA samples and by 3 orders of magnitude in the case of dehydrated Sn-BEA samples as compared to conventional methods. In the latter case, the reconstruction of the quantitative spectrum without the loss of sensitivity is shown to be possible. The method proposed allows obtaining (119)Sn MAS NMR spectra with improved resolution for Sn-BEA zeolites with natural (119)Sn isotope abundance using conventional MAS NMR equipment. PMID:26978430

  7. Investigation on Acute Biochemical Effects of Ce(NO3)3 on Liver and Kidney Tissues by MAS 1H NMR Spectroscopic-Based Metabonomic Approach

    2006-01-01

    High resolution magic angle spinning (MAS)-1 H nuclear magnetic resonance (NMR) spectroscopic-based metabonomic approach was applied to the investigation on the acute biochemical effects of Ce(NO3)3. Male Wistar rats were liver and kidney tissues were analyzed using principal component analysis to extract toxicity information. The biochemical effects of Ce(NO3)3 were characterized by the increase of triglycerides and lactate and the decrease of glycogen in rat liver tissue, together with an elevation of the triglyceride level and a depletion of glycerophosphocholine and betaine in kidney tissues. The target lesions of Ce(NO3)3 on liver and kidney were found by MAS NMR-based metabonomic method. This study demonstrates that the combination of MAS 1H NMR and pattern recognition analysis can be an effective method for studies of biochemical effects of rare earths.

  8. Downregulation of ACE2/Ang-(1-7)/Mas axis promotes breast cancer metastasis by enhancing store-operated calcium entry.

    Yu, Changhui; Tang, Wei; Wang, Yuhao; Shen, Qiang; Wang, Bin; Cai, Chunqing; Meng, Xiaojing; Zou, Fei

    2016-07-01

    The renin-angiotensin system (RAS) is an important component of the tumor microenvironment and plays a key role in promoting cancer cell proliferation, angiogenesis, metabolism, migration and invasion. Meanwhile, the arm of angiotensin-converting enzyme (ACE)2/angiotensin-(1-7) [Ang-(1-7)]/Mas axis in connection with RAS is associated with anti-proliferative, vasodilatory and anti-metastatic properties. Previous studies have shown that Ang-(1-7) reduces the proliferation of orthotopic human breast tumor growth by inhibiting cancer-associated fibroblasts. However, the role of ACE/Ang-(1-7)/Mas axis in the metastasis of breast cancer cells is still unknown. In the present study, we found that ACE2 protein level is negatively correlated with the metastatic ability of breast cancer cells and breast tumor grade. Upregulation of ACE2/Ang-(1-7)/Mas axis inhibits breast cancer cell migration and invasion in vivo and in vitro. Mechanistically, ACE2/Ang-(1-7)/Mas axis activation inhibits store-operated calcium entry (SOCE) and PAK1/NF-κB/Snail1 pathways, and induces E-cadherin expression. In summary, our results demonstrate that downregulation of ACE2/Ang-(1-7)/Mas axis stimulates breast cancer metastasis through the activation of SOCE and PAK1/NF-κB/Snail1 pathways. These results provide new mechanisms by which breast cancer develop metastasis and shed light on developing novel anti-metastasis therapeutics for metastatic breast cancer by modulating ACE2/Ang-(1-7)/Mas axis. PMID:27063099

  9. Hydration kinetics for the alite, belite, and calcium aluminate phase in Portland cements from 27Al and 29Si MAS NMR spectroscopy

    Skibsted, Jørgen; Jensen, Ole Mejlhede; Jakobsen, Hans Jørgen

    1997-01-01

    29Si magic-angle spinning (MAS) NMR spectroscopy is shown to be a valuable tool for obtaining the quantities of alite and belite in hydrated Portland cements. The hydration (1-180 days) of a white Portland cement with 10 wt.% silica fume added is investigated and the degrees of hydration for alit...... belite, and silica fume are determined. It is demonstrated that 27Al MAS NMR spectra of hydrated Portland cements can give quantitative information about the formation of ettringite and the conversion of this phase to monosulphate during hydration....

  10. Tagging of blast resistance gene(s) to DNA markers and marker-assisted selection (MAS) in rice improvement

    This paper reports progress made on the tagging of blast resistance gene(s) to DNA markers and on the initiation of marker-assisted selection (MAS) for blast resistance in rice improvement. A pair of near isogenic lines, K8OR and K79S, were developed using a Chinese landrace Hong-jiao-zhan as the resistance donor. Ten putatively positive markers were identified by screening 177 mapped DNA markers. Using the F2 population of 143 plants and the derived F3 lines, three Restriction Fragment Length Polymorphism (RFLP) markers (RG81, RG869 and RZ397) on chromosome 12 of rice were identified to be closely linked to the blast resistance gene Pi-12(t). The genetic distance between Pi-12(t) and the closest marker RG869 was 5.1 cM. By employing the bulk segregant analysis (BSA) procedure, six of 199 arbitrary primers were found to produce positive Randomly Amplified Polymorphic DNA (RAPD) bands. Tight linkage between Pi-12(t) and three RAPD bands, each from a different primer, was confirmed after amplification of DNA of all F2 individuals. Two fragments were cloned and sequenced, and two sequence characterised amplified re-ion (SCAR) markers were established. In two other F3 populations, Xian-feng I/Tetep and Xian-feng, 1/Hong-jiao-zhan, the blast resistance was found to be controlled by interactions of two or more genes. One resistance gene was located in the vicinity of RG81 in both populations. Work to identify other gene(s) is currently under way. Marker assisted selection for blast resistance was initiated. Crosses were made between elite varieties and blast resistance donors to develop populations for DNA marker-assisted selection of blast resistance. In addition, 48 varieties widely used in current rice breeding programs were provided by rice breeders. DNA marker-based polymorphism among, these varieties and resistance donors were analysed to produce a database for future MAS program. (author)

  11. Dynamic effects in MAS and MQMAS NMR spectra of half-integer quadrupolar nuclei: calculations and an application to the double perovskite cryolite.

    Kotecha, Mrignayani; Chaudhuri, Santanu; Grey, Clare P; Frydman, Lucio

    2005-11-30

    Dynamic processes such as chemical exchange or rotations between inequivalent orientations can affect the magic-angle spinning (MAS) and the multiple-quantum (MQ) MAS NMR spectra of half-integer quadrupolar nuclei. The present paper discusses such dynamic multisite MAS and MQMAS effects and applies them to study the dynamic processes that occur in the double perovskite cryolite, Na3AlF6. Dynamic line shape simulations invoking a second-order broadening of the central transition and relying on the semiclassical Bloch-McConnell formalism for chemical exchange were performed for a variety of exchange models possessing different symmetries. Fitting experimental variable-temperature cryolite 23Na NMR data with this formalism revealed that the two inequivalent sodium sites in this mineral undergo an exchange characterized by a broad distribution of rates. To further assess this dynamic process a variety of 27Al and 19F MAS NMR studies were also undertaken; quantitative 27Al-19F dipolar coupling measurements then revealed a dynamic motion of the AlF6 octahedra that were qualitatively consistent with predictions stemming from molecular dynamic simulations on this double perovskite. PMID:16305261

  12. Protein resonance assignment at MAS frequencies approaching 100 kHz: a quantitative comparison of J-coupling and dipolar-coupling-based transfer methods

    Penzel, Susanne; Smith, Albert A.; Agarwal, Vipin; Hunkeler, Andreas [ETH Zürich, Physical Chemistry (Switzerland); Org, Mai-Liis; Samoson, Ago, E-mail: ago.samoson@ttu.ee [Tallinn University of Technology, NMR Instituut, Tartu Teadus, Tehnomeedikum (Estonia); Böckmann, Anja, E-mail: a.bockmann@ibcp.fr [UMR 5086 CNRS/Université de Lyon 1, Institut de Biologie et Chimie des Protéines (France); Ernst, Matthias, E-mail: maer@ethz.ch; Meier, Beat H., E-mail: beme@ethz.ch [ETH Zürich, Physical Chemistry (Switzerland)

    2015-10-15

    We discuss the optimum experimental conditions to obtain assignment spectra for solid proteins at magic-angle spinning (MAS) frequencies around 100 kHz. We present a systematic examination of the MAS dependence of the amide proton T{sub 2}′ times and a site-specific comparison of T{sub 2}′ at 93 kHz versus 60 kHz MAS frequency. A quantitative analysis of transfer efficiencies of building blocks, as they are used for typical 3D experiments, was performed. To do this, we compared dipolar-coupling and J-coupling based transfer steps. The building blocks were then combined into 3D experiments for sequential resonance assignment, where we evaluated signal-to-noise ratio and information content of the different 3D spectra in order to identify the best assignment strategy. Based on this comparison, six experiments were selected to optimally assign the model protein ubiquitin, solely using spectra acquired at 93 kHz MAS. Within 3 days of instrument time, the required spectra were recorded from which the backbone resonances have been assigned to over 96 %.

  13. Application of 119Sn CPMG MAS NMR for Fast Characterization of Sn Sites in Zeolites with Natural 119Sn Isotope Abundance

    G. Kolyagin, Yury; V. Yakimo, Alexander; Tolborg, Søren;

    2016-01-01

    119Sn CPMG MAS NMR is demonstrated to be a fast and efficient method for characterization of Sn-sites in Sn-containing zeolites. Tuning of the CPMG echo-train sequence decreases the experimental time by a factor of 5–40 in the case of as-synthesized and hydrated Sn-BEA samples and by 3 orders of...

  14. KrasMAS: Implementation of a nuclear material computerized accounting system at the Mining and Chemical Combine through the Russian/US cooperative MPC and A program

    The Russian/US Mining and Chemical Combine (Gorno-Kimichesky Kombinat, GKhK, also referred to as Krasnoyarsk-26) Material Protection, Control and Accounting (MPC and A) project was initiated in June 1996. A critical component of the ongoing cooperative MPC and A enhancements at the GKhK is the implementation of a computerized nuclear material control and accountability (MC and A) system. This system must meet the MC and A requirements of the GKhK by integrating the information generated by numerous existing and new MC and A components in place at the GKhK (e.g., scales, bar-code equipment, NDA measurement systems). During the first phase of this effort, the GKhK adapted CoreMAS (developed at Los Alamos National Laboratory) for use in the PuO2 storage facility. This included formulation of Web-based user interfaces for plant personnel, Russification of the existing user interface, and at the functional level, modification of the CoreMAS stored procedures. The modified system is referred to as KrasMAS and builds upon completed work on CoreMAS. Ongoing efforts include adding GKhK specific report forms and expanding the functionality of the system for implementation at the radiochemical processing and reactor plants of the GKhK. Collaborations with other Russian facilities for appropriate parts of these efforts will be pursued

  15. Procyanidins in fruit from Sour cherry (Prunus cerasus) differ strongly in chainlength from those in Laurel cherry (Prunus lauracerasus) and Cornelian cherry (Cornus mas)

    Capanoglu, E.; Boyacioglu, D.; Vos, de R.C.H.; Hall, R.D.; Beekwilder, M.J.

    2011-01-01

    Sour cherry (Prunus cerasus), Laurel cherry (Prunus lauracerasus), and Cornelian cherry (Cornus mas) fruits are widely used in Turkey, both as food and as traditional medicines. The phytochemical composition and antioxidant capacities of these three cherry types were compared. Fruit flesh was evalua

  16. 2D 23Na-23Na DQ/MAS NMR spectroscopy: interface induced clustering and immobilization of sodium ions in nanostructured aluminosilicates

    Kobera, Libor; Urbanová, Martina; Brus, Jiří

    International Society of Magnetic Resonance, 2015. P 112. [Alpine Conference on Solid-State NMR /9./. 13.09.2015-17.09.2015, Chamonix Mont-Blanc] R&D Projects: GA ČR(CZ) GA13-24155S Institutional support: RVO:61389013 Keywords : MAS NMR * geopolymers * zeolites Subject RIV: JN - Civil Engineering

  17. Size-dependent scanning parameters (kVp and mAs) for photon-counting spectral CT system in pediatric imaging: simulation study

    Chen, Han; Danielsson, Mats; Xu, Cheng

    2016-06-01

    We are developing a photon-counting spectral CT detector with a small pixel size of 0.4× 0.5 mm2, offering a potential advantage for better visualization of small structures in pediatric patients. The purpose of this study is to determine the patient size dependent scanning parameters (kVp and mAs) for pediatric CT in two imaging cases: adipose imaging and iodinated blood imaging. Cylindrical soft-tissue phantoms of diameters between 10–25 cm were used to mimic patients of different ages from 0 to 15 y. For adipose imaging, a 5 mm diameter adipose sphere was assumed as an imaging target, while in the case of iodinated imaging, an iodinated blood sphere of 1 mm in diameter was assumed. By applying the geometry of a commercial CT scanner (GE Lightspeed VCT), simulations were carried out to calculate the detectability index, {{d}\\prime 2} , with tube potentials varying from 40 to 140 kVp. The optimal kVp for each phantom in each imaging case was determined such that the dose-normalized detectability index, {{d}\\prime 2}/ dose, is maximized. With the assumption that the detectability index in pediatric imaging is required the same as in typical adult imaging, the value of mAs at optimal kVp for each phantom was selected to achieve a reference detectability index that was obtained by scanning an adult phantom (30 cm in diameter) in a typical adult CT procedure (120 kVp and 200 mAs) using a modeled energy-integrating system. For adipose imaging, the optimal kVps are 50, 60, 80, and 120 kVp, respectively, for phantoms of 10, 15, 20, and 25 cm in diameter. The corresponding mAs values required to achieve the reference detectability index are only 9%, 23%, 24%, and 54% of the mAs that is used for adult patients at 120 kVp, for 10, 15, 20, and 25 cm diameter phantoms, respectively. In the case of iodinated imaging, a tube potential of 60 kVp was found optimal for all phantoms investigated, and the mAs values required to achieve the reference detectability

  18. CP-MAS 207Pb with 19F decoupling NMR spectroscopy: medium range investigation in fluoride materials.

    Bureau, B; Silly, G; Buzaré, J Y

    1999-11-01

    The isotropic chemical shift of 207Pb is used to perform structural investigations of crystalline fluoride compounds (PbF2, Pb2ZnF6, PbGaF5, Pb3Ga2F12 and Pb9Ga2F24) and transition metal fluoride glasses (TMFG) of the PZG family (PbF2-ZnF2-GaF3). Using 207Pb Cross Polarisation Magic Angle Spinning (CP-MAS) NMR with 19F decoupling, it is shown that the isotropic chemical shift of 207Pb varies on a large scale (1000 ppm) and that the main changes of its value are not due to the nearest neighbour fluorines but may be related to the number of next nearest neighbour (nnn) Pb2+ ions. In this way, it is demonstrated that 207Pb chemical shift is an interesting probe to investigate medium range order in either crystalline or glassy fluoride systems. The 207Pb delta(iso) parameter has been linearly correlated to the number of nnn Pb2+ ions. PMID:10670899

  19. Effect of physiographic factors on qualitative and quantitative characteristics of Cornus mas L. natural stands in Arasbaran forests, Iran

    Ahmad Alijanpour

    2013-01-01

    Arasbaran forests are located in East Azerbaijan (northwest Iran).Increasing of socio-economic problems in this area causes destruction of biodiversity and structure of these forests.Using multipurpose trees such as cornelian cherry (Cornus mas L) to encourage villagers to produce forest by-products is a basic approach for preserving these forests.This species grows naturally in Arasbaran forests and the fruit is annually exploited using traditional harvest methods.This study aims to assess the ecological requirements of cornelian cherry and the important factors affecting its distribution.For this purpose,40 circular sampling plots (300 m2) on various slope aspects were demarcated for sampling the occurrence of cornelian cherry in forest stands.DBH and crown cover percentage on north aspects were significantly greater than on other aspects and 4.5% of all trees were cornelian cherry in mature forest stands.North aspects had more seed-origin trees (standards) of cornelian cherry than coppiced trees,while west facing aspects had more coppiced than standard trees.This species had the highest regeneration rate in the sapling stage of 0-2.5 cm DBH.Thus,I recommend cultivation and development of cornelian cherry as a multi-purpose tree in the Arasbaran region on degraded forest lands on north and west aspects.

  20. Structural investigations of PuIII phosphate by X-ray diffraction, MAS-NMR and XANES spectroscopy

    PuPO4 was prepared by a solid state reaction method and its crystal structure at room temperature was solved by powder X-ray diffraction combined with Rietveld refinement. High resolution XANES measurements confirm the +III valence state of plutonium, in agreement with valence bond derivation. The presence of the americium (as β− decay product of plutonium) in the +III oxidation state was determined based on XANES spectroscopy. High resolution solid state 31P NMR agrees with the XANES results and the presence of a solid-solution. - Graphical abstract: A full structural analysis of PuPO4 based on Rietveld analysis of room temperature X-ray diffraction data, XANES and MAS NMR measurements was performed. - Highlights: • The crystal structure of PuPO4 monazite is solved. • In PuPO4 plutonium is strictly trivalent. • The presence of a minute amount of AmIII is highlighted. • We propose PuPO4 as a potential reference material for spectroscopic and microscopic studies

  1. Etkin liderlik ve takım çalışması = Effective leadership and teamwork

    Gökçe, Hakan

    2011-01-01

    Uzun süre önce işletme ve kurumlara giren ve hızla yaygınlaşan takım çalışmaları, çok boyutlu sorunlarda bile çözümün ve başarının anahtarı halini almıştır. Takım çalışmasındaki başarı üst yönetimin bakış açısıyla bağlantılı olmakla birlikte, takım elemanlarının ilişkileri, gönüllü olmaları ve iş birliğine olan yatkınlıklarıyla da alakalıdır. Bu noktada lider faktörü kendini hissettirmektedir. Liderin takım içindeki etkinliği başarıyı sağlayacak faktörlerin başında gelir. Bu çalışmada bir tak...

  2. N Photo-CIDNP MAS NMR To Reveal Functional Heterogeneity in Electron Donor of Different Plant Organisms.

    Janssen, Geertje J; Roy, Esha; Matysik, Jörg; Alia, A

    2012-02-01

    In plants and cyanobacteria, two light-driven electron pumps, photosystems I and II (PSI, PSII), facilitate electron transfer from water to carbon dioxide with quantum efficiency close to unity. While similar in structure and function, the reaction centers of PSI and PSII operate at widely different potentials with PSI being the strongest reducing agent known in living nature. Photochemically induced dynamic nuclear polarization (photo-CIDNP) in magic-angle spinning (MAS) nuclear magnetic resonance (NMR) measurements provides direct excess to the heart of large photosynthetic complexes (A. Diller, Alia, E. Roy, P. Gast, H.J. van Gorkom, J. Zaanen, H.J.M. de Groot, C. Glaubitz, J. Matysik, Photosynth. Res. 84, 303-308, 2005; Alia, E. Roy, P. Gast, H.J. van Gorkom, H.J.M. de Groot, G. Jeschke, J. Matysik, J. Am. Chem. Soc. 126, 12819-12826, 2004). By combining the dramatic signal increase obtained from the solid-state photo-CIDNP effect with (15)N isotope labeling of PSI, we were able to map the electron spin density in the active cofactors of PSI and study primary charge separation at atomic level. We compare data obtained from two different PSI proteins, one from spinach (Spinacia oleracea) and other from the aquatic plant duckweed (Spirodella oligorrhiza). Results demonstrate a large flexibility of the PSI in terms of its electronic architecture while their electronic ground states are strictly conserved. PMID:22303078

  3. Proton-detected scalar coupling based assignment strategies in MAS solid-state NMR spectroscopy applied to perdeuterated proteins

    Linser, Rasmus; Fink, Uwe; Reif, Bernd

    2008-07-01

    Assignment of proteins in MAS (magic angle spinning) solid-state NMR relies so far on correlations among heteronuclei. This strategy is based on well dispersed resonances in the 15N dimension. In many complex cases like membrane proteins or amyloid fibrils, an additional frequency dimension is desirable in order to spread the amide resonances. We show here that proton detected HNCO, HNCA, and HNCACB type experiments can successfully be implemented in the solid-state. Coherences are sufficiently long lived to allow pulse schemes of a duration greater than 70 ms before incrementation of the first indirect dimension. The achieved resolution is comparable to the resolution obtained in solution-state NMR experiments. We demonstrate the experiments using a triply labeled sample of the SH3 domain of chicken α-spectrin, which was re-crystallized in H 2O/D 2O using a ratio of 1/9. We employ paramagnetic relaxation enhancement (PRE) using EDTA chelated Cu II to enable rapid data acquisition.

  4. Role of meconium in the reaction of airways smooth musculature in the newborn with meconium aspiration syndrome (MAS).

    Islami, Hilmi; Bexheti, Sadi; Shabani, Ragip; Nuraj, Bajram; Zeqiri, Fehmi; Sukalo, Aziz; Kurtishi, Ilir; Kutllovci, Skender; Qorraj, Hasime; Disha, Mentor

    2009-11-01

    The role of meconium in the respiratory system was studied in newborns, who died from various causes (250 up to 3000 g of weight). We monitored tracheal rings response to dopamine, serotonin and ethanol in different concentrations (dopamine: 0,05 mg/ml, 0,5 mg/ml, 5 mg/ml; serotonin (5-HT): 10-4, 10-3, 10-2, 10-1 mol/dm3; ethanol: 0,02 ml, 0,5 ml, 1,0 ml; 96%). Tracheal smooth musculature tonus (TSM) was examined in 48 tracheal preparations taken after the newborn exitus due to different reasons. Based on functional researche of isolated preparations of tracheas, it may be concluded that: aspiration of meconium has not changed the response of TSM to dopamine, serotonin and ethanol (p>0,1) in comparison with the control group, which have died due to different lung inflammatory processes (e.g. pneumonia, bronchopneumonia, atelectasis, cerebral hemorrhage). The results suggest that meconium does not potentiate the constricting action of dopamine, serotonin and ethanol in tracheobronchial system. Meconium causes mild relaxation of the TSM through a mechanism that is not intermediated by the products of cyclooxygenases (prostaglandins, prostacyclins) from the tracheal epithelium or proteins. Also, as it seems, the direct activity of many tested acids in the smooth musculature has no significant impact on increase of the airways tonus in MAS syndrome. PMID:20002002

  5. Structural investigations of Pu{sup III} phosphate by X-ray diffraction, MAS-NMR and XANES spectroscopy

    Popa, Karin [European Commission, Joint Research Centre, Institute for Transuranium Elements, P.O. Box 2340, D-76125 Karlsruhe (Germany); Raison, Philippe E., E-mail: philippe.raison@ec.europa.eu [European Commission, Joint Research Centre, Institute for Transuranium Elements, P.O. Box 2340, D-76125 Karlsruhe (Germany); Martel, Laura [European Commission, Joint Research Centre, Institute for Transuranium Elements, P.O. Box 2340, D-76125 Karlsruhe (Germany); Martin, Philippe M. [CEA, DEN, DEC/SESC, F-13108 Saint Paul Lez Durance Cedex (France); Prieur, Damien [European Commission, Joint Research Centre, Institute for Transuranium Elements, P.O. Box 2340, D-76125 Karlsruhe (Germany); Solari, Pier L. [Synchrotron SOLEIL, 91190 Saint-Aubin (France); Bouëxière, Daniel; Konings, Rudy J.M.; Somers, Joseph [European Commission, Joint Research Centre, Institute for Transuranium Elements, P.O. Box 2340, D-76125 Karlsruhe (Germany)

    2015-10-15

    PuPO{sub 4} was prepared by a solid state reaction method and its crystal structure at room temperature was solved by powder X-ray diffraction combined with Rietveld refinement. High resolution XANES measurements confirm the +III valence state of plutonium, in agreement with valence bond derivation. The presence of the americium (as β{sup −} decay product of plutonium) in the +III oxidation state was determined based on XANES spectroscopy. High resolution solid state {sup 31}P NMR agrees with the XANES results and the presence of a solid-solution. - Graphical abstract: A full structural analysis of PuPO{sub 4} based on Rietveld analysis of room temperature X-ray diffraction data, XANES and MAS NMR measurements was performed. - Highlights: • The crystal structure of PuPO{sub 4} monazite is solved. • In PuPO{sub 4} plutonium is strictly trivalent. • The presence of a minute amount of Am{sup III} is highlighted. • We propose PuPO{sub 4} as a potential reference material for spectroscopic and microscopic studies.

  6. Quality of spelt pasta enriched with eggs and identification of eggs using 13C MAS NMR spectroscopy

    Filipović Jelena S.

    2015-01-01

    Full Text Available This paper deals with the characteristics of spelt pasta enriched with eggs. Eggs were added to spelt farina in the quantity of 0, 124 or 248 g/kg (equivalent to 0, 3 or 6 eggs, respectively. Post-hoc Tukey’s HSD test at 95% confidence limit showed significant differences between various samples. Relatively low coefficients of variation have been obtained for each applied assay (1.25-12.42%, which confirmed the high accuracy measurements and statistically significant results. Standard score analysis is applied for accessing the contribution of eggs content to spelt pasta quality. Maximum scores regarding quality (0.89 and chemical characteristics (0.70, have been obtained for 6 eggs spelt pasta formulation. It is also shown that the presence of eggs in pasta can be clearly confirmed by 13C MAS NMR spectroscopy. Simultaneous increase in area of peak positioned at 29.5 and 176 ppm is directly associated with the increase in the content of added eggs in the corresponding samples. Pertinent data point at positive contribution of eggs to the spelt pasta and also that NMR spectrum can be used in the egg quantity control. [Projekat Ministarstva nauke Republike Srbije, br. TRI 46005 i br. TR 31029

  7. El universo mas pequeno

    Rivera, Alicia

    2007-01-01

    The future of the exploration of the microcosmos will be in a ring formed by more than 8'000 magnets. With the new great particle acceleator LHC, nearly finished in Geneva, scientists will see how the universe was created just after the Big Bang. (3 pages with photos)

  8. Local environments and lithium adsorption on the iron oxyhydroxides lepidocrocite (gamma-FeOOH) and goethite (alpha-FeOOH): A 2H-2 and 7Li solid-state MAS NMR study

    Nielsen, Ulla Gro; Grey, Clare P.; Paik, Jonkim

    2008-01-01

    2H and 7LiMAS NMR spectroscopy techniques were applied to study the local surface and bulk environments of iron oxyhydroxide lepiclocrocite (gamma-FeOOH). 2H variable-temperature (VT) MAS NMR experiments were performed, showing the presence of short-range, strong antiferromagnetic correlations, e...

  9. Conceptualización y análisis de un sistema multi-agente pedagógico utilizando la metodología mas-commonkads./ Conceptualization and analysis of a pedagogical multi-agent system using mas-commonkads methodology.

    JIMÉNEZ BUILES, JOVANI ALBERTO; Ovalle Carranza, Demetrio Arturo; Branch Bedoya, John Willian

    2009-01-01

    En este artículo se presentan la conceptualización y el análisis de un sistema multi-agente (MAS) pedagógico utilizando la metodología MAS-CommonKADS; la cual es una de las más reconocidas para el modelado de MAS. El propósito de la fase de conceptualización es comprender el problema, identificar los actores activos y pasivo, además de elaborar los requisitos que permitan formular unos objetivos para generar la solución. En la fase de análisis se definen los modelos de agente, tareas, coordin...

  10. Development of a homogeneous calcium mobilization assay for high throughput screening of mas-related gene receptor agonists

    Rui ZHANG; Pang-ke YAN; Cai-hong ZHOU; Jia-yu LIAO; Ming-wei WANG

    2007-01-01

    Aim: To develop homogeneous calcium mobilization assay for high-throughput screening (HTS) of mas-related gene (Mrg) receptor agonists. Methods: CHO-K1 cells stably expressing the full-length MrgD receptor and a calcium-sensitive dye were used to develop an HTS assay based on intracellular calcium influx. This method was applied to large-scale screening of a library containing 8000 synthetic compounds and natural product extracts, cAMP measurements were camed out to verify the bioactivities of the hits found by the calcium mobilization assay. Similar approaches were also employed in the identification of the MrgA1 recep-tor agonists following HTS of 16 000 samples. Results: EC50 values of the positive control compounds (β-alanine for MrgD receptor and dynorphin A for MrgA1 receptor) determined by the calcium mobilization assay were consistent with those reported in the literature, and the Z' factors were 0.65 and 0.50 for MrgD and MrgA1 receptor assay, respectively. About 31 compounds for the MrgD receptor and 48 compounds for the MrgA1 receptor showing ≥20% of the maximal agonist activities found in the controls were initially identified as hits. Secondary screen- ing confirmed that 2 compounds for each receptor possessed specific agonist activities. Intracellular cAMP level measurements indicated that the 2 confirmed hits displayed the functionality of the MrgD receptor agonists. Conclusion: A series of validation studies demonstrated that the homogeneous calcium mobili-zation assay developed was highly efficient, amenable to automation and a robust tool to screen potential MrgD and MrgA1 receptor agonists. Its application may be expanded to other G-protein coupled receptors that mobilize calcium influx upon activation.

  11. Optimum levels of exchangeable protons in perdeuterated proteins for proton detection in MAS solid-state NMR spectroscopy

    Akbey, Umit; Lange, Sascha; Trent Franks, W.; Linser, Rasmus; Rehbein, Kristina; Diehl, Anne; Rossum, Barth-Jan van; Reif, Bernd; Oschkinat, Hartmut, E-mail: oschkinat@fmp-berlin.d [Leibniz-Forschungsinstitut fuer Molekulare Pharmakologie (FMP) (Germany)

    2010-01-15

    We present a systematic study of the effect of the level of exchangeable protons on the observed amide proton linewidth obtained in perdeuterated proteins. Decreasing the amount of D{sub 2}O employed in the crystallization buffer from 90 to 0%, we observe a fourfold increase in linewidth for both {sup 1}H and {sup 15}N resonances. At the same time, we find a gradual increase in the signal-to-noise ratio (SNR) for {sup 1}H-{sup 15}N correlations in dipolar coupling based experiments for H{sub 2}O concentrations of up to 40%. Beyond 40%, a significant reduction in SNR is observed. Scalar-coupling based {sup 1}H-{sup 15}N correlation experiments yield a nearly constant SNR for samples prepared with {<=}30% H{sub 2}O. Samples in which more H{sub 2}O is employed for crystallization show a significantly reduced NMR intensity. Calculation of the SNR by taking into account the reduction in {sup 1}H T{sub 1} in samples containing more protons (SNR per unit time), yields a maximum SNR for samples crystallized using 30 and 40% H{sub 2}O for scalar and dipolar coupling based experiments, respectively. A sensitivity gain of 3.8 is obtained by increasing the H{sub 2}O concentration from 10 to 40% in the CP based experiment, whereas the linewidth only becomes 1.5 times broader. In general, we find that CP is more favorable compared to INEPT based transfer when the number of possible {sup 1}H,{sup 1}H interactions increases. At low levels of deuteration ({>=}60% H{sub 2}O in the crystallization buffer), resonances from rigid residues are broadened beyond detection. All experiments are carried out at MAS frequency of 24 kHz employing perdeuterated samples of the chicken {alpha}-spectrin SH3 domain.

  12. Schémas variationnels et gestion du non-standard en contextes français et maltais

    Bezzina Anne-Marie

    2016-01-01

    Full Text Available Une visée comparative est adoptée dans cette étude traitant de la variation, notamment de la variation stylistique qui se produit en fonction du degré de formalité caractérisant la situation de communication et d’un nombre d’autres critères. La comparaison des situations sociolinguistiques présentes en contextes français et maltais permet de faire ressortir les caractéristiques propres à chaque situation. En français, la variation stylistique passe par le registre; en maltais, l’alternance codique maltais/anglais s’y ajoute pour la réalisation du style. Le français se distingue par une ampleur stylistique exceptionnelle, qui s’explique par diverses raisons culturelles, comme l’idéologie du standard et la portée de la littératie. Les deux cadres s’opposent par leurs processus de standardisation: le français, anciennement élu langue officielle, a naturellement suivi des schémas de progrès différents du maltais, langue officielle depuis bien moins longtemps, à l’héritage écrit qui ne remonte pas très loin dans le passé. Les deux langues partagent pourtant certains objectifs qui sous-tendent leurs efforts de protection. Des hypothèses sont émises quant à l’acceptabilité sociale de la variation aux différents niveaux d’analyse linguistique en maltais, et quant au type de variation proéminent dans cette langue, qui serait la variation sociale. Ces hypothèses sont construites par comparaison avec les arguments parallèles élaborés pour la situation française.

  13. 13C chemical shift anisotropies for carbonate ions in cement minerals and the use of 13C, 27Al and 29Si MAS NMR in studies of Portland cement including limestone additions

    13C isotropic chemical shifts and chemical shift anisotropy parameters have been determined for a number of inorganic carbonates relevant in cement chemistry from slow-speed 13C MAS or 13C(1H) CP/MAS NMR spectra (9.4 T or 14.1 T) for 13C in natural abundance. The variation in the 13C chemical shift parameters is relatively small, raising some doubts that different carbonate species in Portland cement-based materials may not be sufficiently resolved in 13C MAS NMR spectra. However, it is shown that by combining 13C MAS and 13C(1H) CP/MAS NMR carbonate anions in anhydrous and hydrated phases can be distinguished, thereby providing valuable information about the reactivity of limestone in cement blends. This is illustrated for three cement pastes prepared from an ordinary Portland cement, including 0, 16, and 25 wt.% limestone, and following the hydration for up to one year. For these blends 29Si MAS NMR reveals that the limestone filler accelerates the hydration for alite and also results in a smaller fraction of tetrahedrally coordinated Al incorporated in the C-S-H phase. The latter result is more clearly observed in 27Al MAS NMR spectra of the cement–limestone blends and suggests that dissolved aluminate species in the cement–limestone blends readily react with carbonate ions from the limestone filler, forming calcium monocarboaluminate hydrate. -- Highlights: •13C chemical shift anisotropies for inorganic carbonates from 13C MAS NMR. •Narrow 13C NMR chemical shift range (163–171 ppm) for inorganic carbonates. •Anhydrous and hydrated carbonate species by 13C MAS and 13C(1H) CP/MAS NMR. •Limestone accelerates the hydration for alite in Portland – limestone cements. •Limestone reduces the amount of aluminium incorporated in the C-S-H phase

  14. 1H and 23Na MAS NMR spectroscopy of cationic species in CO2 selective alkaline earth metal porous silicoaluminophosphates prepared via liquid and solid state ion exchange

    Arévalo-Hidalgo, Ana G.; Dugar, Sneha; Fu, Riqiang; Hernández-Maldonado, Arturo J.

    2012-07-01

    The location of extraframework cations in Sr2+ and Ba2+ ion-exchanged SAPO-34 was estimated by means of 1H and 23Na MAS NMR spectroscopy and spectral deconvolution. Incorporation of the alkaline earth metal cations onto the SAPO framework was achieved via liquid state ion exchange, coupled partial detemplation/solid-state ion exchange, and combination of both techniques. MAS NMR revealed that the level of ion exchange was limited by the presence of protons and sodium cations near hexagonal prisms (site SI), which are relatively difficult to exchange with the alkaline earth metal due to steric and charge repulsion criteria. In addition, the presence of ammonium cations in the supercages facilitated the exchange of otherwise tenacious hydrogen as corroborated by unit cell compositional data as well as enhanced CO2 adsorption at low partial pressures. The extraframework ammonium species were produced from partial detemplation of the structure-directing agent employed for the SAPO-34 synthesis, tetraethylammonium.

  15. Coal structure at reactive sites by sup 1 H- sup 13 C- sup 19 F double cross polarization (DCP)/MAS sup 13 C NMR spectroscopy

    Hagaman, E.W.; Woody, M.C. (Oak Ridge National Lab., TN (USA))

    1989-01-01

    The solid state NMR technique, {sup 1}H-{sup 13}C-{sup 31}P double cross polarization (DCP)/MAS {sup 13}C-NMR spectroscopy, uses the direct dipolar interaction between {sup 13}C-{sup 31}P spin pairs in organophosphorus substances to identify the subset of carbons within a spherical volume element of 0.4 nm radius centered on the {sup 31}P atom. In combination with chemical manipulation of coals designed to introduce phosphorus containing functionality into the organic matrix, the NMR experiment becomes a method to examine selectively the carbon bonding network at the reactive sites in the coal. This approach generates a statistical structure description of the coal at the reaction centers in contrast to bulk carbon characterization using conventional {sup 1}H-{sup 13}C CP/MAS {sup 13}C NMR spectroscopy. 3 refs.

  16. 1H and 23Na MAS NMR spectroscopy of cationic species in CO2 selective alkaline earth metal porous silicoaluminophosphates prepared via liquid and solid state ion exchange

    The location of extraframework cations in Sr2+ and Ba2+ ion-exchanged SAPO-34 was estimated by means of 1H and 23Na MAS NMR spectroscopy and spectral deconvolution. Incorporation of the alkaline earth metal cations onto the SAPO framework was achieved via liquid state ion exchange, coupled partial detemplation/solid-state ion exchange, and combination of both techniques. MAS NMR revealed that the level of ion exchange was limited by the presence of protons and sodium cations near hexagonal prisms (site SI), which are relatively difficult to exchange with the alkaline earth metal due to steric and charge repulsion criteria. In addition, the presence of ammonium cations in the supercages facilitated the exchange of otherwise tenacious hydrogen as corroborated by unit cell compositional data as well as enhanced CO2 adsorption at low partial pressures. The extraframework ammonium species were produced from partial detemplation of the structure-directing agent employed for the SAPO-34 synthesis, tetraethylammonium. - Graphical abstract: MAS NMR was used to elucidate the position the cationic species in alkaline earth metal exchanged silicoaluminophosphates. These species played a significant role during the ion exchange process and, therefore, the materials ultimate CO2 adsorption performance. Highlights: ► Location of extraframework Sr2+ or Ba2+ cations was estimated by means of 1H and 23Na MAS NMR. ► Level of Sr2+ or Ba2+ ion exchange was limited by the presence of protons and sodium cations. ► Presence of ammonium cations in the supercages facilitated the exchange. ► Sr2+ and Ba2+ ion exchanged SAPOs are outstanding CO2 adsorbents.

  17. Organisational Culture in Innovative Small to Medium Sized Enterprises (SMEs) : Leadership’s Responsibilities when Implementing Change as a Result of M&As

    Rasmussen, Sara

    2013-01-01

    This study investigates what culturally related responsibilities leaders in innovative SMEs have when preparing employees prior to M&As in order to mitigate drawbacks due to culturally related discrepancies. The findings of this study especially confirm previous studies on culturally related difficulties in change management by emphasising the significant meaning of evaluating soft factors prior to change. The study was conducted by examining an innovative SME in the telecom industry that...

  18. Application of High-Resolution 1H MAS NMR Spectroscopy to the Analysis of Intact Bones from Mice Exposed to Gamma Radiation

    Zhang, QiBin; Hu, Jian Zhi; Rommereim, Donald N.; Murphy, Mark K; Phipps, Richard P.; HUSO, DAVID L.; Dicello, John F

    2009-01-01

    Herein we demonstrate that high-resolution magic angle spinning (MAS) 1H NMR can be used to profile the pathology of bone marrow rapidly and with minimal sample preparation. The spectral resolution obtained allows several metabolites to be analyzed quantitatively. The level of NMR-detectable metabolites in the epiphysis + metaphysis sections of mouse femur were significantly higher than that observed in the diaphysis of the same femur. The major metabolite damage to bone marrow resulting from...

  19. Quantification of Choline- and Ethanolamine-Containing Metabolites in Human Prostate Tissues Using 1H HR-MAS Total Correlation Spectroscopy

    Swanson, Mark G.; Keshari, Kayvan R.; Tabatabai, Z. Laura; Simko, Jeffry P.; Shinohara, Katsuto; Carroll, Peter R.; Zektzer, Andrew S.; Kurhanewicz, John

    2008-01-01

    A fast and quantitative 2D high-resolution magic angle spinning (HR-MAS) total correlation spectroscopy (TOCSY) experiment was developed to resolve and quantify the choline- and ethanolamine-containing metabolites in human prostate tissues in ≈1 hr prior to pathologic analysis. At a 40-ms mixing time, magnetization transfer efficiency constants were empirically determined in solution and used to calculate metabolite concentrations in tissue. Phosphocholine (PC) was observed in 11/15 (73%) can...

  20. Hydrogen-bond interactions in organically-modified polysiloxane networks studied by 1D and 2D CRAMPS and double-quantum 1H MAS NMR

    Brus, Jiří; Dybal, Jiří

    2002-01-01

    Roč. 35, č. 27 (2002), s. 10038-10047. ISSN 0024-9297 R&D Projects: GA ČR GA203/98/P290; GA AV ČR KSK4050111 Institutional research plan: CEZ:AV0Z4050913 Keywords : Hydrogen bonding * polysiloxane * 1H MAS NMR Subject RIV: CD - Macromolecular Chemistry Impact factor: 3.751, year: 2002

  1. Attenuation of Mycobacterium tuberculosis by Disruption of a mas-Like Gene or a Chalcone Synthase-Like Gene, Which Causes Deficiency in Dimycocerosyl Phthiocerol Synthesis

    Sirakova, Tatiana D.; Dubey, Vinod S.; Cynamon, Michael H.; Kolattukudy, Pappachan E.

    2003-01-01

    Tuberculosis is one of the leading preventable causes of death. Emergence of drug-resistant tuberculosis makes the discovery of new targets for antimycobacterial drugs critical. The unique mycobacterial cell wall lipids are known to play an important role in pathogenesis, and therefore the genes responsible for their biosynthesis offer potential new targets. To assess the possible role of some of the genes potentially involved in cell wall lipid synthesis, we disrupted a mas-like gene, msl7, ...

  2. Ease of delignification assesment of wood from different Eucalyptus species by pyrolysis (TMAH)-GC/MS and CP/MAS 13C-NMR spectrometry

    González-Vila, Francisco Javier; Almendros Martín, Gonzalo; Río Andrade, José Carlos del; Martín Martínez, Francisco; Gutiérrez Suárez, Ana; Romero Sánchez, Juan

    1999-01-01

    Flash-pyrolysis in the presence of tetramethylammonium hydroxide (TMAH) of woods from different species of Eucalyptus yields series of guaiacyl-type (G) and syringyl-type (S) units in slightly but characteristically different relative proportions. Such differences have been used to suggest a fine and appropriate index of the ease of delignification of the different Eucalyptus species when pulped by the Kraft process. The pyrolytic data were in agreement with those obtained from CP/MAS 13C-NMR...

  3. Mejora de la eficiencia energética en la explotación ganadera "Mas Isern" situada en el término municipal de Vic

    Canga Navarro, Jordi

    2014-01-01

    The objective of the present paper is to describe the energy situation, as well as, analyze and evaluate proposals for improvement of the exploitation of dairy cattle, Mas Isern, located in Vic's municipal area. To comply with this objective was conducted an energy audit as a tool of compilation and analysis of information. The energy audit evaluates mainly the turnover of the electrical rate of power supply, the sizing and the energy efficiency of the lights of the stable, the performance of...

  4. Optimal degree of protonation for {sup 1}H detection of aliphatic sites in randomly deuterated proteins as a function of the MAS frequency

    Asami, Sam [Helmholtz-Zentrum Muenchen (HMGU), Deutsches Forschungszentrum fuer Gesundheit und Umwelt (HMGU) (Germany); Szekely, Kathrin; Schanda, Paul; Meier, Beat H. [Eidgenoessische Technische Hochschule Zuerich (ETH Zuerich) (Switzerland); Reif, Bernd, E-mail: reif@tum.de [Helmholtz-Zentrum Muenchen (HMGU), Deutsches Forschungszentrum fuer Gesundheit und Umwelt (HMGU) (Germany)

    2012-10-15

    The {sup 1}H dipolar network, which is the major obstacle for applying proton detection in the solid-state, can be reduced by deuteration, employing the RAP (Reduced Adjoining Protonation) labeling scheme, which yields random protonation at non-exchangeable sites. We present here a systematic study on the optimal degree of random sidechain protonation in RAP samples as a function of the MAS (magic angle spinning) frequency. In particular, we compare {sup 1}H sensitivity and linewidth of a microcrystalline protein, the SH3 domain of chicken {alpha}-spectrin, for samples, prepared with 5-25 % H{sub 2}O in the E. coli growth medium, in the MAS frequency range of 20-60 kHz. At an external field of 19.96 T (850 MHz), we find that using a proton concentration between 15 and 25 % in the M9 medium yields the best compromise in terms of sensitivity and resolution, with an achievable average {sup 1}H linewidth on the order of 40-50 Hz. Comparing sensitivities at a MAS frequency of 60 versus 20 kHz, a gain in sensitivity by a factor of 4-4.5 is observed in INEPT-based {sup 1}H detected 1D {sup 1}H,{sup 13}C correlation experiments. In total, we find that spectra recorded with a 1.3 mm rotor at 60 kHz have almost the same sensitivity as spectra recorded with a fully packed 3.2 mm rotor at 20 kHz, even though {approx}20 Multiplication-Sign less material is employed. The improved sensitivity is attributed to {sup 1}H line narrowing due to fast MAS and to the increased efficiency of the 1.3 mm coil.

  5. Chemometric analysis applied in 1H HR-MAS NMR and FT-IR data for chemotaxonomic distinction of intact lichen samples

    This paper describes the potentiality of chemometric analysis applied in 1H HR-MAS NMR and FT-IR data for lichen chemotaxonomic investigations. Lichens present a difficult morphologic differentiation and the chemical analyses are frequently employed for their taxonomic classification, mainly due to the secondary metabolites to be relatively constant for these organisms. The lichen chemotaxonomic classification is usually carried out by color reactions, chromatography, fluorescence and mass spectrometry analysis, where the identification is obtained by one or more techniques. There are some papers which use the carbohydrate content in chemotaxonomy investigation. However, the majority of these techniques involve laborious and time consuming sample pre-treatment. This work focuses on application of 1H high resolution magic angle spinning - nuclear magnetic resonance (HR-MAS NMR) and Fourier transform infrared (FT-IR) associated with chemometric analysis to intact samples. In comparison to other traditional techniques, 1H HR-MAS NMR and FT-IR allied with chemometrics provided a fast and economic method for lichen chemotaxonomy. Both methods were useful for lichen analysis and permitted the satisfactory distinction among families, genera and species, although better results were achieved for FT-IR data

  6. Research on the Shipborne Close-in System Based on the MAS Technolohy%基于MAS技术的舰载近防系统的研究

    卢道华; 穆兴兴

    2011-01-01

    Combining carrier-based air defense missiles, main artillery, near defense artillery and jamming devices to form a near defense system is proposed based on MAS technology, and the common task is achieved through coordinating in ternal Agents, so as to the MAS near defense system becomes a intelligent, near-defense and carrier-based system that can self improve, gather experience and is shareable. The concepts, principles, work process and key technologies of MAS near defense system are described in detail.%提出将舰载防空导弹、主炮、近防炮与干扰装置组建为一个基于MAS技术的近防系统。通过协调系统内部各Agent共同完成任务,使系统成为能在工作过程中自我完善、经验积累和分享的智能舰载近防系统。阐述了MAS近防系统的概念、原理、工作过程及其主要关键技术。

  7. Sensitivity enhancement in natural-abundance solid-state 33S MAS NMR spectroscopy employing adiabatic inversion pulses to the satellite transitions

    Hansen, Michael Ryan; Brorson, Michael; Bildsøe, Henrik; Skibsted, Jørgen; Jakobsen, Hans J.

    2008-02-01

    The WURST (wideband uniform rate smooth truncation) and hyperbolic secant (HS) pulse elements have each been employed as pairs of inversion pulses to induce population transfer (PT) between the four energy levels in natural abundance solid-state 33S (spin I = 3/2) MAS NMR, thereby leading to a significant gain in intensity for the central transition (CT). The pair of inversion pulses are applied to the satellite transitions for a series of inorganic sulfates, the sulfate ions in the two cementitious materials ettringite and thaumasite, and the two tetrathiometallates (NH 4) 2WS 4 and (NH 4) 2MoS 4. These materials all exhibit 33S quadrupole coupling constants ( CQ) in the range 0.1-1.0 MHz, with precise CQ values being determined from analysis of the PT enhanced 33S MAS NMR spectra. The enhancement factors for the WURST and HS elements are quite similar and are all in the range 1.74-2.25 for the studied samples, in excellent agreement with earlier reports on HS enhancement factors (1.6-2.4) observed for other spin I = 3/2 nuclei with similar CQ values (0.3-1.2 MHz). Thus, a time saving in instrument time by a factor up to five has been achieved in natural abundance 33S MAS NMR, a time saving which is extremely welcome for this important low-γ nucleus.

  8. Evaluation of 10 mAs and low-contrast CT image optimization based on the multifractal spectrum in brain of infant

    Objective: To analyze scanned image optimization based on the multifractal soectrum and image fractal algorithm of 64-slice spiral CT in brain of infant. Methods: The image data of Toshiba Aquilion 64-slice CT scanning using 10 mAs were imported to image processing toolboxs of Matlab 7.1. The evaluation of multifractal spectrum and image denosing were performed, and compared with image quality of conventional low-dose CT using 50 mAs. Results: The low-contrast scanned image used 10 mAs is the valueless medical image because of serious noise. Image denoise based on the fractal model had superior characteristic of image detail preserving and better contrast-to-noise ratio(CNR). There existed a group difference in the score of image quality between the rude imaging noise and optimized image based on the multifractal spectrum algorithm, though the score was still significantly lower than the normal dosage scanned image (F=38.85, P<0.01). The group difference was also manifested the image quality of infants can achieve basically the request of clinical diagnosis by suitable model denoising algorithm. Conclusions: Image denoising based on the multifractal spectrum model can be used on the low-dose and low-contrast CT image optimization. It improved the CNR of the pathological region. The radiation dose of CT scanning in infants would be declined significantly by its further application in the future. (authors)

  9. Sensitivity of Neurospora crassa to a Marine-Derived Aspergillus tubingensis Anhydride Exhibiting Antifungal Activity That Is Mediated by the MAS1 Protein

    Liat Koch

    2014-09-01

    Full Text Available The fungus Aspergillus tubingensis (strain OY907 was isolated from the Mediterranean marine sponge Ircinia variabilis. Extracellular extracts produced by this strain were found to inhibit the growth of several fungi. Among the secreted extract components, a novel anhydride metabolite, tubingenoic anhydride A (1 as well as the known 2-carboxymethyl-3-hexylmaleic acid anhydride, asperic acid, and campyrone A and C were purified and their structure elucidated. Compound 1 and 2-carboxymethyl-3-hexylmaleic acid anhydride inhibited Neurospora crassa growth (MIC = 330 and 207 μM, respectively and affected hyphal morphology. We produced a N. crassa mutant exhibiting tolerance to 1 and found that a yet-uncharacterized gene, designated mas-1, whose product is a cytosolic protein, confers sensitivity to this compound. The ∆mas-1 strain showed increased tolerance to sublethal concentrations of the chitin synthase inhibitor polyoxin D, when compared to the wild type. In addition, the expression of chitin synthase genes was highly elevated in the ∆mas-1 strain, suggesting the gene product is involved in cell wall biosynthesis and the novel anhydride interferes with its function.

  10. Structural nature of 7Li and 11B sites in the nonlinear optical material LiB3O5 using static NMR and MAS NMR

    The structural nature of the nonlinear optical properties of LiB3O5 is analyzed using single-crystal nuclear magnetic resonance (NMR) and magic angle spinning (MAS) NMR. The 3-coordinated trigonal [B(1) and B(2)] and 4-coordinated tetragonal [B(3)] sites are distinguished using the spectrum and the spin-lattice relaxation time in rotating frame T1ρ, which was obtained from the 11B MAS NMR. Moreover, the T1 and T1ρ values for 7Li and 11B are compared, and the activation energies were obtained. The T1ρ values of the boron nuclei in LiB3O5 show no significant changes. These results may be closely related to the largest second-order nonlinear optical coefficient. - Highlights: • The structural nature of the nonlinear optical properties of LiB3O5. • Single-crystal NMR and MAS NMR. • The 3-coordnated trigonal and 4-coordinated tetragonal. • The spin-lattice relaxation time in rotating frame T1ρ

  11. Remote Sensing of Liquid Water and Ice Cloud Optical Thickness and Effective Radius in the Arctic: Application of Airborne Multispectral MAS Data

    King, Michael D.; Platnick, Steven; Yang, Ping; Arnold, G. Thomas; Gray, Mark A.; Riedi, Jerome C.; Ackerman, Steven A.; Liou, Kuo-Nan

    2003-01-01

    A multispectral scanning spectrometer was used to obtain measurements of the reflection function and brightness temperature of clouds, sea ice, snow, and tundra surfaces at 50 discrete wavelengths between 0.47 and 14.0 microns. These observations were obtained from the NASA ER-2 aircraft as part of the FIRE Arctic Clouds Experiment, conducted over a 1600 x 500 km region of the north slope of Alaska and surrounding Beaufort and Chukchi Seas between 18 May and 6 June 1998. Multispectral images of the reflection function and brightness temperature in 11 distinct bands of the MODIS Airborne Simulator (MAS) were used to derive a confidence in clear sky (or alternatively the probability of cloud), shadow, and heavy aerosol over five different ecosystems. Based on the results of individual tests run as part of the cloud mask, an algorithm was developed to estimate the phase of the clouds (water, ice, or undetermined phase). Finally, the cloud optical thickness and effective radius were derived for both water and ice clouds that were detected during one flight line on 4 June. This analysis shows that the cloud mask developed for operational use on MODIS, and tested using MAS data in Alaska, is quite capable of distinguishing clouds from bright sea ice surfaces during daytime conditions in the high Arctic. Results of individual tests, however, make it difficult to distinguish ice clouds over snow and sea ice surfaces, so additional tests were added to enhance the confidence in the thermodynamic phase of clouds over the Beaufort Sea. The cloud optical thickness and effective radius retrievals used 3 distinct bands of the MAS, with the newly developed 1.62 and 2.13 micron bands being used quite successfully over snow and sea ice surfaces. These results are contrasted with a MODIS-based algorithm that relies on spectral reflectance at 0.87 and 2.13 micron.

  12. Intelligent Optimizing Control of Formation for UUVs Based on MAS%基于MAS的多UUV编队智能优化控制

    李宏宏; 康凤举; 顾浩

    2013-01-01

    The multi-agent system (MAS) based strategy and corresponding implementing algorithms for UUVs formation navigation are proposed according to aviation characteristics and practical const raint s of UUVs. The coordinative optimizations among every individual UUV are carried out with flexible and smart ways by interactive communications and information feedback in MAS so as to implement the autonomous formation navigation of UUVs. Furthermore the control law and corresponding algorithms of the UUV controller in the formation process are studied in depth based on the controller in the formation keeping and simulation result s verify the robustness and stability of the formation cont rollers. Finally, the formation intelligent control method was verified by simulated verification and comparative analysis.%针对无人水下航行器(Unmanned Underwater Vehicle,UUV)编队航行的实际背景和限制条件,提出了一种基于多智能体系统(MAS)技术的多UUV编队智能控制方法.利用多Agent之间的交互作用,以灵活便捷的方式进行各UUV之间的协同优化,从而实现多个UUV的自主编队航行.考虑到控制器在编队保持中的重要性,对面向UUV编队航行的控制率和实现算法进行了重点研究,设计了UUV编队控制器,并通过仿真实验验证了该算法的鲁棒性和稳定性,最后对多UUV编队智能控制方法进行了仿真验证和分析.

  13. Combined high-field 13C CP MAS NMR and low-field NMR relaxation measurements on post mortem porcine muscles.

    Bertram, Hanne Christine; Jakobsen, Hans Jørgen; Andersen, Henrik Jørgen

    2004-05-19

    Changes in postmortem muscle characteristics are investigated in muscles from eight pigs exposed to different combinations of preslaughter stress (exercise on treadmill) and stunning method (CO(2) vs electrical stunning). Solid-state (13)C cross-polarization (CP) magic-angle spinning (MAS) NMR experiments are carried out on a total of 16 rapidly frozen M. longissimus muscle biopsies taken in vivo the day before slaughter and at 45 min postmortem. Simultaneously, low-field NMR T(2) relaxation time measurements are carried out on samples from M. longissimus. Glycogen and lactate are estimated from the (13)C CP MAS spectra, and correlations of r = 0.89 and r = 0.70, respectively, to subsequent biochemical determinations using partial least squares regression (PLSR) are established. Moreover, PLSR reveals that, besides the 72 ppm signal (carbons in glycogen), a signal around 38 ppm, which increases concomitantly with lactate, is also significantly correlated to changes in glycogen/lactate. With the assumption that the 38 ppm signal reflects CH(2) in phosphocreatine/creatine, altered mobility of creatine as a result of dephosphorylation is indicated. Finally, PLSR on the 45 min (13)C CP MAS spectra also reveals correlation (r = 0.54) to the slowest relaxing T(2) population (50 min postmortem), known to reflect extra-myofibrillar water. Subsequently, evaluation of the loading plot in the PLSR analysis reveals that the correlation exclusively is associated to the 52 ppm resonance intensity. With the assumption that this resonance reflects methyl groups in choline/phosphatidyl choline, the intensity changes in the 52 ppm resonance imply alterations in membrane properties. Accordingly, the data indicate a relationship between membrane properties and the amount of water being expelled from muscle cells postmortem, which supports the hypothesis that disruption of membranes is implicated in the postmortem mobilization of muscle water. PMID:15137869

  14. ATIVIDADE EPILINGUÍSTICA E O ENSINO DE LÍNGUA MATERNA: UM EXERCÍCIO COM A CONJUNÇÃO "MAS"

    Camila Arndt Wamser; Letícia Marcondes Rezende

    2013-01-01

    O texto discute a importância do trabalho com a atividade epilinguística, entendida como a própria atividade de linguagem, no ensino de língua materna. O conceito de atividade epilinguística que adotamos é o da Teoria das Operações Predicativas e Enunciativas, elaborada pelo linguista francês Antoine Culioli. Com o intuito de delimitar o objeto de pesquisa e apresentar um modelo de atividade aplicado em sala de aula, trabalhamos com a conjunção adversativa mas, concebendo-a como marcador léxi...

  15. Corrado Neri, Âges inquiets. Cinémas chinois: une représentation de la jeunesse (Uneasy ages. Chinese cinemas: A representation of youth)

    Pernin, Judith

    2010-01-01

    Corrado Neri, Âges inquiets. Cinémas chinois: une représentation de la jeunesse (Uneasy ages. Chinese cinemas: A representation of youth), Lyon, Tigre de Papier, 2009, 533 pp. This work by Corrado Neri, based on his doctoral thesis, deals with the thematic treatment of youth in "Chinese language cinema." It is a historical and trans-territorial study of the representation of the youth in the cinematography of Taiwan, Hong Kong, and the People's Republic. By the term "youth," which may appear ...

  16. Prise en compte des schémas cognitifs dans la scénarisation des activités d'enseignement

    Dessus, Philippe; Pernin, Jean-Philippe; Lejeune, Anne

    2007-01-01

    Cet article met en perspective deux champs de recherche indépendants, celui de l'étude de la cognition des enseignants et celui de la scénarisation pédagogique, autour d'un objet central : le schéma. Nous passons tout d'abord en revue les différents types de schémas (routines, scripts, recettes), pour montrer ensuite comment ils sont mis en oeuvre dans l'activité d'enseignement, et plus particulièrement à quatre fins : agir en situation, planifier l' enseignement, comprendre une situation et ...

  17. Global Fold of Human Cannabinoid Type 2 Receptor Probed by Solid-State 13C-, 15N-MAS NMR and Molecular Dynamics Simulations

    Kimura, Tomohiro; Vukoti, Krishna; Lynch, Diane L.; Hurst, Dow P.; Grossfield, Alan; Pitman, Michael C.; Reggio, Patricia H.; Yeliseev, Alexei A.; Gawrisch, Klaus

    2013-01-01

    The global fold of human cannabinoid type 2 (CB2) receptor in the agonist-bound active state in lipid bilayers was investigated by solid-state 13C- and 15N magic-angle spinning (MAS) NMR, in combination with chemical-shift prediction from a structural model of the receptor obtained by microsecond-long molecular dynamics (MD) simulations. Uniformly 13C-, and 15N-labeled CB2 receptor was expressed in milligram quantities by bacterial fermentation, purified, and functionally reconstituted into l...

  18. Uso del FitoMas-E® como atenuante del estrés salino (NaCl durante la emergencia y crecimiento inicial de Ocimum basilicum L.

    D. Batista-Sánchez

    2015-01-01

    Full Text Available El uso de bioestimulantes vegetales estimula la emergencia y el crecimiento vegetal debido a que están compuestos por sustancias naturales como carbohidratos, péptidos de bajo peso molecular y aminoácidos, activadores de las funciones fisiológicas de las plantas, por lo que su aplicación permite un mejor aprovechamiento de los nutrientes y representa una opción para enfrentar problemas de estrés abiótico por salinidad. El objetivo de este estudio fue determinar el efecto del bioestimulante FitoMas-E® derivado de la caña de azúcar como atenuante de la salinidad en la emergencia y el crecimiento de plántulas de variedades de albahaca (Ocimum basilicum L. con respuesta diferencial al estrés por salinidad. Tres variedades de albahaca: Napoletano, Emily y Nufar se sometieron a cuatro concentraciones de NaCl (0, 50, 100 y 150 mM y cuatro dosis de FitoMas-E® (0, 0.5, 1.0 y 1.5 mL L-1 en un diseño completamente al azar con arreglo factorial con cuatro repeticiones. El trabajo se desarrolló en una estructura de malla sombra y las variables que se midieron fueron: porcentaje y tasa de emergencia, longitud de radícula, altura de la plántula, biomasa fresca y seca de radícula y de parte aérea. Los resultados evidenciaron que en condiciones de estrés salino (NaCl de moderado a severo, las variables disminuyeron significativamente. Largo de raíz, altura de planta y biomasa seca de raíz en 150 mM de NaCl fueron las más afectadas. Se observó que cuando se aplicó FitoMas-E® las plantas incrementaron significativamente sus valores en las variables, largo de raíz 32% y altura de planta 41%, lo que revela el efecto estimulante del FitoMas-E®, siendo 0.5 mL L-1 la dosis estimulante para la variedad Napoletano y 1.0 mL L-1 para la variedad Emily, aún y cuando se encontraban en condiciones de estrés salino hasta 100 mM de NaCl. Para la concentración de 150 mM se evidenció una afectación extrema para las variedades Emily y Nufar.

  19. The study of a monocotyledon abscission zone using microscopic, chemical, enzymatic and solid state 13C CP/MAS NMR analyses.

    Henderson, J; Davies, H A; Heyes, S J; Osborne, D J

    2001-01-01

    We have investigated distinguishing features in cells of the abscission zone of a monocotyledon fruit, the oil palm Elaeis guineensis. The cell walls of the abscission zone and the subtending mesocarp and pedicel have been analysed by light and transmission electron microscopy, by chemical methods and by solid state 13C CP/MAS NMR spectroscopy. Results show that these abscission zone cells have specific characteristics which include high levels of unmethylated pectin in the walls and an inducible (x35) polygalacturonase enzyme expression. Together these findings help to explain the localised precision of cell separation events. PMID:11219806

  20. 1H-MAS-NMR Chemical Shifts in Hydrogen-Bonded Complexes of Chlorophenols (Pentachlorophenol, 2,4,6-Trichlorophenol, 2,6-Dichlorophenol, 3,5-Dichlorophenol, and p-Chlorophenol) and Amine, and H/D Isotope Effects on 1H-MAS-NMR Spectra

    Hisashi Honda

    2013-01-01

    Chemical shifts (CS) of the 1H nucleus in N···H···O type hydrogen bonds (H-bond) were observed in some complexes between chlorophenols [pentachlorophenol (PCP), 2,4,6-tricholorophenol (TCP), 2,6-dichlorophenol (26DCP), 3,5-dichlorophenol (35DCP), and p-chlorophenol (pCP)] and nitrogen-base (N-Base) by solid-state high-resolution 1H-NMR with the magic-angle-spinning (MAS) method. Employing N-Bases with a wide range of pKa values (0.65–10.75), 1H-MAS-NMR CS values of bridging H atoms in H-bonds...

  1. 1H-MAS-NMR Chemical Shifts in Hydrogen-Bonded Complexes of Chlorophenols (Pentachlorophenol, 2,4,6-Trichlorophenol, 2,6-Dichlorophenol, 3,5-Dichlorophenol, and p-Chlorophenol and Amine, and H/D Isotope Effects on 1H-MAS-NMR Spectra

    Hisashi Honda

    2013-04-01

    Full Text Available Chemical shifts (CS of the 1H nucleus in N···H···O type hydrogen bonds (H-bond were observed in some complexes between chlorophenols [pentachlorophenol (PCP, 2,4,6-tricholorophenol (TCP, 2,6-dichlorophenol (26DCP, 3,5-dichlorophenol (35DCP, and p-chlorophenol (pCP] and nitrogen-base (N-Base by solid-state high-resolution 1H-NMR with the magic-angle-spinning (MAS method. Employing N-Bases with a wide range of pKa values (0.65–10.75, 1H-MAS-NMR CS values of bridging H atoms in H-bonds were obtained as a function of the N-Base’s pKa. The result showed that the CS values were increased with increasing pKa values in a range of DpKa 2: The maximum CS values was recorded in the PCP (pKa = 5.26–4-methylpyridine (6.03, TCP (6.59–imidazole (6.99, 26DCP (7.02–2-amino-4-methylpyridine (7.38, 35DCP (8.04–4-dimethylaminopyridine (9.61, and pCP (9.47–4-dimethylaminopyridine (9.61 complexes. The largest CS value of 18.6 ppm was recorded in TCP–imidazole crystals. In addition, H/D isotope effects on 1H-MAS-NMR spectra were observed in PCP–2-amino-3-methylpyridine. Based on the results of CS simulation using a B3LYP/6-311+G** function, it can be explained that a little changes of the N–H length in H-bond contribute to the H/D isotope shift of the 1H-MAS-NMR peaks.

  2. Temperature {sup 1}H, {sup 13}C, {sup 15}N NMR and CP/MAS {sup 15}N NMR spectra of benzotriazole derivatives - prototropic tautomerism; Widma temperaturowe {sup 1}H, {sup 13}C, {sup 15}N NMR oraz CP/MAS {sup 15}N NMR pochodnych benzotriazolu - tautomeria prototropowa

    Wiench, J.W.; Stefaniak, L. [Inst. Chemii Organicznej, Polska Akademia Nauk, Warsaw (Poland)

    1994-12-31

    The prototropic tautomerism in benzotriazole derivatives solutions has been investigated in different temperatures by means of {sup 1}H, {sup 13}C and {sup 15}N NMR and {sup 15}N CP/MAS NMR spectra. The ratio of different tautomeric forms and kinetics of proton exchange have been measured for the systems studied on the base of observed spectroscopic factors. 5 refs, 2 figs, 3 tabs.

  3. Obtaining aluminas from the thermal decomposition of their different precursors: An {sup 27}Al MAS NMR and X-ray powder diffraction studies

    Chagas, L.H.; De Carvalho, G.S.G. [Universidade Federal de Juiz de Fora, Departamento de Química, Grupo de Físico-Química de Sólidos e Interfaces, 36036-330 Juiz de Fora, MG (Brazil); San Gil, R.A.S. [Universidade Federal do Rio de Janeiro, Instituto de Química, 21949-900 Rio de Janeiro, RJ (Brazil); Chiaro, S.S.X. [PETROBRAS-CENPES, 21941-915 Rio de Janeiro, RJ (Brazil); Leitão, A.A. [Universidade Federal de Juiz de Fora, Departamento de Química, Grupo de Físico-Química de Sólidos e Interfaces, 36036-330 Juiz de Fora, MG (Brazil); Diniz, R., E-mail: renata.diniz@ufjf.edu.br [Universidade Federal de Juiz de Fora, Departamento de Química, Grupo de Físico-Química de Sólidos e Interfaces, 36036-330 Juiz de Fora, MG (Brazil)

    2014-01-01

    Graphical abstract: - Highlights: • We synthesized three precursors of alumina from different methods. • The calcination of the precursors generated several alumina polymorphs. • XRD and NMR were used for structural investigation of the polymorphs. • The synthesis route determines the structural and textural properties of the solids. - Abstract: A commercial sample of Boehmite was used as precursor of alumina polymorphs. For comparison, three other precursors were synthesized from different methods. Particularly, the use of excess of urea promoted a very crystalline form of basic aluminum carbonate. The characteristics of the four precursors were investigated by thermal, vibrational and X-ray powder diffraction (XRD) analysis. Additionally, the nuclear magnetic resonance, with magic angle spinning ({sup 27}Al MAS NMR), was used to verify the coordination of aluminum cations. Each precursor was calcined at various temperatures generating alumina polymorphs, which were structurally analyzed by XRD and {sup 27}Al MAS NMR. Due to interest in catalysis supports, special attention was given to the γ-Al{sub 2}O{sub 3} phase, which in addition to structural investigation was subjected to textural analysis. The results showed that, from different synthesis procedures and common route of calcination, one can obtain materials with the same composition but with different structural and textural properties, which in turn can significantly influence the performance of a supported catalyst.

  4. Windows 10 reklāmas kampaņas lokalizācijas analīze Latvijā 2015. gadā

    Strogonova, Sanita

    2016-01-01

    Bakalaura darbā “Windows 10 reklāmas kampaņas lokalizācijas analīze Latvijā 2015. gadā” tiek pētīta Microsoft korporācijas veiktās globālās un lokalizētās integrētā mārketinga komunikācijas zīmola Windows 10 virzīšanai Latvijas tirgū. Darbs sastāv no 3 daļām: teorētiskās daļas, metodoloģijas daļas un empīriskās daļas. Teorijas daļā ir veikts literatūras apskats par komunikācijas modeļu veidiem, integrētā mārketinga teorijām, apskatīta reklāmas kampaņas veidošanas un efektivitātes mērīšanas te...

  5. Modification of molybdenum structural environment in borosilicate glasses with increasing content of boron and calcium oxide by 95Mo MAS NMR

    In nuclear borosilicate glasses, when molybdenum is in too high concentration and when it combines with other elements such as alkali and alkaline-earth elements it may form crystalline molybdates, including sodium molybdate, Na2MoO4, during melt cooling. In a nuclear vitrification context, the origin of this phenomenon must be understood to control and to avoid the appearance of this water-soluble crystalline phase. The solubility limit of MoO3 was found to be 2.5 mol% in a simplified SiO2-B2O3-Na2O-CaO nuclear glass at about 1300 degrees C. Higher MoO3 concentrations induced liquid phase separation followed by crystallization of Na2MoO4 and CaMoO4. This study assessed the impact of increasing the CaO and B2O3 content on the tendency of the melts to crystallize and the impact on the glass network structure. Structural analysis (Mo-95 MAS NMR and B-11 MAS NMR) of several glass series and standard SiO2-Na2O-MoO3 or SiO2-CaO-MoO3 glass showed that the nature of the crystallized phases that may appear during cooling of the melt can be controlled by correlation of the proportion of Na+ cations remaining free in the glass network with the soda/lime environment of tetrahedral MoO42- entities. (authors)

  6. Modification of Molybdenum Structural Environment in Borosilicate Glasses with Increasing Content of Boron and Calcium Oxide by 95Mo MAS NMR

    In nuclear borosilicate glasses, when molybdenum is in too high concentration and when it combines with other elements such as alkali and alkaline-earth elements it may form crystalline molybdates, including sodium molybdate, Na2MoO4, during melt cooling. In a nuclear vitrification context, the origin of this phenomenon must be understood to control and to avoid the appearance of this water-soluble crystalline phase. The solubility limit of MoO3 was found to be 2.5 mol% in a simplified SiO2-B2O3-Na2O-CaO nuclear glass at about 1300 degrees C. Higher MoO3 concentrations induced liquid phase separation followed by crystallization of Na2MoO4 and CaMoO4. This study assessed the impact of increasing the CaO and B2O3 content on the tendency of the melts to crystallize and the impact on the glass network structure. Structural analysis (95Mo MAS NMR and 11B MAS NMR) of several glass series and standard SiO2-Na2O-MoO3 or SiO2-CaO-MoO3 glass showed that the nature of the crystallized phases that may appear during cooling of the melt can be controlled by correlation of the proportion of Na+ cations remaining free in the glass network with the soda/lime environment of tetrahedral MoO42- entities. (authors)

  7. High Field 27Al MAS NMR and TPD studies of active sites in ethanol dehydration using thermally treated transitional aluminas as catalysts

    Hu, Jian Z.; Xu, Suochang; Kwak, Ja Hun; Hu, Mary Y.; Wan, Chuan; Zhao, Zhenchao; Szanyi, Janos; Bao, Xinhe; Han, Xiuwen; Wang, Yong; Peden, Charles HF

    2016-04-04

    High field quantitative 27Al MAS NMR and temperature programmed desorption (TPD) of ethanol are used to study the surface and phase transformation of gamma-Al2O3 during calcination in the temperature range of 500 to 1300 degrees C. Following ethanol adsorption, ethylene is generated during TPD with a desorption temperature > 200 degrees C. With increasing calcination temperature prior to TPD, the amount of ethylene produced decreases monotonically. Significantly, 27Al MAS NMR reveals that the amount of penta-coordinate Al3+ ions (Lewis acid sites) also decreases with increasing calcination temperature. In fact, a strong correlation between the amount of penta-coordinate Al3+ ions and the amount of strongly adsorbed ethanol molecules (i.e., the ones that convert to ethylene during TPD) is obtained. This result indicates that the penta-coordinate aluminum sites are the catalytic active sites on alumina surfaces during ethanol dehydration reaction across the entire course of gamma- to alpha-Al2O3 phase transformations.

  8. Structure determination of uniformly 13C, 15N labeled protein using qualitative distance restraints from MAS solid-state 13C-NMR observed paramagnetic relaxation enhancement

    Magic angle spinning (MAS) solid-state nuclear magnetic resonance (NMR) is a powerful method for structure determination of insoluble biomolecules. However, structure determination by MAS solid-state NMR remains challenging because it is difficult to obtain a sufficient amount of distance restraints owing to spectral complexity. Collection of distance restraints from paramagnetic relaxation enhancement (PRE) is a promising approach to alleviate this barrier. However, the precision of distance restraints provided by PRE is limited in solid-state NMR because of incomplete averaged interactions and intermolecular PREs. In this report, the backbone structure of the B1 domain of streptococcal protein G (GB1) has been successfully determined by combining the CS-Rosetta protocol and qualitative PRE restraints. The derived structure has a Cα RMSD of 1.49 Å relative to the X-ray structure. It is noteworthy that our protocol can determine the correct structure from only three cysteine-EDTA-Mn2+ mutants because this number of PRE sites is insufficient when using a conventional structure calculation method based on restrained molecular dynamics and simulated annealing. This study shows that qualitative PRE restraints can be employed effectively for protein structure determination from a limited conformational sampling space using a protein fragment library

  9. Determination of structural topology of a membrane protein in lipid bilayers using polarization optimized experiments (POE) for static and MAS solid state NMR spectroscopy

    Mote, Kaustubh R. [University of Minnesota, Department of Chemistry (United States); Gopinath, T. [University of Minnesota, Department of Biochemistry, Molecular Biology and Biophysics (United States); Veglia, Gianluigi, E-mail: vegli001@umn.edu [University of Minnesota, Department of Chemistry (United States)

    2013-10-15

    The low sensitivity inherent to both the static and magic angle spinning techniques of solid-state NMR (ssNMR) spectroscopy has thus far limited the routine application of multidimensional experiments to determine the structure of membrane proteins in lipid bilayers. Here, we demonstrate the advantage of using a recently developed class of experiments, polarization optimized experiments, for both static and MAS spectroscopy to achieve higher sensitivity and substantial time-savings for 2D and 3D experiments. We used sarcolipin, a single pass membrane protein, reconstituted in oriented bicelles (for oriented ssNMR) and multilamellar vesicles (for MAS ssNMR) as a benchmark. The restraints derived by these experiments are then combined into a hybrid energy function to allow simultaneous determination of structure and topology. The resulting structural ensemble converged to a helical conformation with a backbone RMSD {approx}0.44 A, a tilt angle of 24 Degree-Sign {+-} 1 Degree-Sign , and an azimuthal angle of 55 Degree-Sign {+-} 6 Degree-Sign . This work represents a crucial first step toward obtaining high-resolution structures of large membrane proteins using combined multidimensional oriented solid-state NMR and magic angle spinning solid-state NMR.

  10. XRD, FTIR and 13C CP/ MAS NMR Studies of Composite Comprising Poly(vinyl acetate)- silylated Si-MCM-41

    A composite structure based on silylated MCM-41 and Poly(vinyl acetate) (PVAc) was synthesized via solution intercalation. Poly(vinyl acetate)-silylated Si- MCM-41 composite were characterized by XRD, FTIR spectroscopy and 13C CP/ MAS NMR in order to determine the compatibility between PVAc and the silicate host. XRD study reveals that the framework of silylated Si-MCM-41 was not altered upon incorporation of PVAc. FTIR study showed that characteristic peak assigned to carbonyl group in PVAc was observed around 1741.6 cm-1 for all the composites indicating the presence of PVAc in the silylated Si-MCM-41. 13C CP/ MAS NMR showed the increase of line width of the peak assigned to C=O carbonyl group indicating the increase in randomness of polymer chains in confined space. The shifting of the C=O carbonyl groups is a sign of the change in chemical environment of the carbonyl owing to the interaction of PVAc with the silica matrix of silylated Si-MCM-41. (author)

  11. An application of active optics to x-ray imaging: X-mas (x-ray milli arc-second) Project

    Tsujimoto, Masahiro; Kitamoto, Shunji; Ohkubo, Yohsuke; Sato, Jun'ichi; Watanabe, Takeshi; Sudoh, Keisuke; Sekiguchi, Akiko; Suga, Kazuharu; Sekiguchi, Hiroyuki

    2006-06-01

    We report the current status of the "X-mas" (X-ray milli-arcsecond) project. X-mas is an application of the AO technology to the X-ray optics, aiming to obtain high-resolution defraction-limited X-ray images. Our X-ray telescope employs the Newton optics with a paraboloid primary and a 31-element deformable secondary mirrors. The aperture of the primary mirror is 80 millimeters with the focal length of 2 meters. Multi-layer coating of the mirrors by silicon and molybdenum realizes a large reflectivity of ~60% for the primary and 30-50% for the secondary mirror at 13.5 nm, which enables us to construct a normal incidence optics at this wavelength. We use a laser guide source and a wave front sensor to optimize the form of the secondary deformable mirror for the purpose of offsetting the large-scale figure errors in the X-ray optics. A back-side illumination X-ray CCD detector manufactured by Hamamatsu Photonics is used for X-ray detections. We have assembled all these elements and started to accumulate data. Closed-loop AO is in operation for the laser guide source. Likely X-ray images are obtained through the telescope. The results in 2005-2006 are presented.

  12. Task Programming of Antiballistic Missile Sensors Based on MAS%基于多智能体的反导传感器任务规划算法

    吴林锋; 王刚; 刘昌云; 杨少春

    2012-01-01

    The characteristics and functions of the sensor resources under the antiballistic missile condition are firstly discussed, Then a sensor task programming system structure which adapts to the anti-ballistic missile requirement is put forward based on the multi-agent system (MAS) of distribute artificial intelligence, and the main functions of each agent in the structure in detail is analyzed. Finally a programming process is presented and the arithmetic of a sensor Agent in the layer of entity is carried out, which provides an effective method for building a high-efficiency and intelligent antimissile sensor network.%首先探讨了反导作战中各类传感器资源的构成特点和功能划分,然后基于多智能体multy-agent system(MAS)的分布式人工智能技术提出了适应反导作战需求的传感器任务规划体系架构,既而深入分析了体系架构中各类agent的主要功能,最后给出了一个实体层传感器的任务规划流程和算法实例,为构建高效率、智能化的反导作战多传感器任务规划技术提供了一种有效的研究方法.

  13. Os desastres podem até cair dos céus... Mas a culpa não é de Deus

    Josué Petrônio Quirino de Oliveira

    2014-12-01

    Full Text Available O propósito desta pesquisa é apresentar uma reflexão sobre o descaso em que a politica de enfrentamento aos desastres naturais vem sendo abordada no Brasil nos últimos 100 anos.  Com uma população cada vez mais urbana as cidades passam a ser o centro não só do poder econômico e político, mas também dos mais diversos eventos decorrentes das mudanças climáticas. Uma série de problemas agrava essa urbanização vulnerável e desencadeiam uma crise socioambiental determinada por fatores sociais, políticos, econômicos, tecnológicos, culturais e ecológicos que, de forma integrada, provocam consequências devastadoras. Inundações, deslizamentos, ciclones, incêndios florestais, enxurradas, estiagens, enchentes, secas e terremotos provocam impactos inesperados e pressionam ainda mais este quadro urbano complexo a uma dinâmica preocupante. O desastre não são as principais ameaças, mas a incapacidade de planejamento e enfrentamento na gestão dos desastres demostraram o descaso com a questão foi tratado no país ao longo de décadas.

  14. Reklāmas analīze un produkcijas virzīšana, izmantojot kompānijas L'Oreal Group piemēru

    Platonova, Viktorija

    2016-01-01

    Diplomdarbs veltīts reklāmas jēdziena pētīšanai, kompānijas L'Oreal Group darbības detalizētākai aplūkošanai, kā arī respondentu aptaujas par šī uzņēmuma reklāmu veikšana un tālāka tās analīze. Šis diplomdarbs sastāv no trīs daļām ar apakšdaļām. Pirmajā daļā autore aplūko reklāmas jēdzienu, tās veidus, metodes, efektivitāti un efektivitātes vadīšanas metodes. Otrajā daļā pievērsta uzmanība skaistuma un kosmētikas nozares un detalizētākai kompānijas L'Oreal Group aplūkošanai un aprakstam, konk...

  15. Solid state structure by X-ray and 13C CP/MAS NMR of new 5-[2-(N,N-dimethylamino)ethoxy]-4,7-dimethylcoumarins

    Ostrowska, Kinga; Maciejewska, Dorota; Dobrzycki, Łukasz; Socha, Pawel

    2016-05-01

    5-[2-(N,N-dimethylamino)ethoxy]-4,7-dimethylcoumarin (1) and 6-acetyl-5-[2-(N,N-dimethylamino)ethoxy]-4,7-dimethylcoumarin (2), structurally related, were synthesized using both conventional and microwave-assisted approach. An impact of acetyl groups on the molecular structure of coumarin derivatives has been examined. Crystals of 2 were investigated using single crystal and powder X-ray diffraction techniques. Compound 2 crystallizes forming two polymorphs (denoted as 2_1 and 2_2), both belonging to P21/c space group. Both polymorphs are comparably stable and can be formed simultaneously during crystallization process. The solid state structure was also analysed using the fully resolved 13C CP/MAS NMR. The double signals with the intensity ratio of about 1:1 which were observed in the 13C CP/MAS NMR spectrum of compound 1 must arise due to the presence of two conformers of 1. In contrast, NMR spectrum recorded for powder mixture of two polymorphs of compound 2 displays no signal splitting. This is related to structural similarities of molecules in both polymorphs.

  16. Determination of structural topology of a membrane protein in lipid bilayers using polarization optimized experiments (POE) for static and MAS solid state NMR spectroscopy

    The low sensitivity inherent to both the static and magic angle spinning techniques of solid-state NMR (ssNMR) spectroscopy has thus far limited the routine application of multidimensional experiments to determine the structure of membrane proteins in lipid bilayers. Here, we demonstrate the advantage of using a recently developed class of experiments, polarization optimized experiments, for both static and MAS spectroscopy to achieve higher sensitivity and substantial time-savings for 2D and 3D experiments. We used sarcolipin, a single pass membrane protein, reconstituted in oriented bicelles (for oriented ssNMR) and multilamellar vesicles (for MAS ssNMR) as a benchmark. The restraints derived by these experiments are then combined into a hybrid energy function to allow simultaneous determination of structure and topology. The resulting structural ensemble converged to a helical conformation with a backbone RMSD ∼0.44 Å, a tilt angle of 24° ± 1°, and an azimuthal angle of 55° ± 6°. This work represents a crucial first step toward obtaining high-resolution structures of large membrane proteins using combined multidimensional oriented solid-state NMR and magic angle spinning solid-state NMR

  17. Constant-time 2D and 3D through-bond correlation NMR spectroscopy of solids under 60 kHz MAS

    Zhang, Rongchun; Ramamoorthy, Ayyalusamy

    2016-01-01

    Establishing connectivity and proximity of nuclei is an important step in elucidating the structure and dynamics of molecules in solids using magic angle spinning (MAS) NMR spectroscopy. Although recent studies have successfully demonstrated the feasibility of proton-detected multidimensional solid-state NMR experiments under ultrafast-MAS frequencies and obtaining high-resolution spectral lines of protons, assignment of proton resonances is a major challenge. In this study, we first re-visit and demonstrate the feasibility of 2D constant-time uniform-sign cross-peak correlation (CTUC-COSY) NMR experiment on rigid solids under ultrafast-MAS conditions, where the sensitivity of the experiment is enhanced by the reduced spin-spin relaxation rate and the use of low radio-frequency power for heteronuclear decoupling during the evolution intervals of the pulse sequence. In addition, we experimentally demonstrate the performance of a proton-detected pulse sequence to obtain a 3D 1H/13C/1H chemical shift correlation spectrum by incorporating an additional cross-polarization period in the CTUC-COSY pulse sequence to enable proton chemical shift evolution and proton detection in the incrementable t1 and t3 periods, respectively. In addition to through-space and through-bond 13C/1H and 13C/13C chemical shift correlations, the 3D 1H/13C/1H experiment also provides a COSY-type 1H/1H chemical shift correlation spectrum, where only the chemical shifts of those protons, which are bonded to two neighboring carbons, are correlated. By extracting 2D F1/F3 slices (1H/1H chemical shift correlation spectrum) at different 13C chemical shift frequencies from the 3D 1H/13C/1H spectrum, resonances of proton atoms located close to a specific carbon atom can be identified. Overall, the through-bond and through-space homonuclear/heteronuclear proximities determined from the 3D 1H/13C/1H experiment would be useful to study the structure and dynamics of a variety of chemical and biological

  18. Constant-time 2D and 3D through-bond correlation NMR spectroscopy of solids under 60 kHz MAS

    Zhang, Rongchun; Ramamoorthy, Ayyalusamy, E-mail: ramamoor@umich.edu [Biophysics and Department of Chemistry, The University of Michigan, Ann Arbor, Michigan 48109-1055 (United States)

    2016-01-21

    Establishing connectivity and proximity of nuclei is an important step in elucidating the structure and dynamics of molecules in solids using magic angle spinning (MAS) NMR spectroscopy. Although recent studies have successfully demonstrated the feasibility of proton-detected multidimensional solid-state NMR experiments under ultrafast-MAS frequencies and obtaining high-resolution spectral lines of protons, assignment of proton resonances is a major challenge. In this study, we first re-visit and demonstrate the feasibility of 2D constant-time uniform-sign cross-peak correlation (CTUC-COSY) NMR experiment on rigid solids under ultrafast-MAS conditions, where the sensitivity of the experiment is enhanced by the reduced spin-spin relaxation rate and the use of low radio-frequency power for heteronuclear decoupling during the evolution intervals of the pulse sequence. In addition, we experimentally demonstrate the performance of a proton-detected pulse sequence to obtain a 3D {sup 1}H/{sup 13}C/{sup 1}H chemical shift correlation spectrum by incorporating an additional cross-polarization period in the CTUC-COSY pulse sequence to enable proton chemical shift evolution and proton detection in the incrementable t{sub 1} and t{sub 3} periods, respectively. In addition to through-space and through-bond {sup 13}C/{sup 1}H and {sup 13}C/{sup 13}C chemical shift correlations, the 3D {sup 1}H/{sup 13}C/{sup 1}H experiment also provides a COSY-type {sup 1}H/{sup 1}H chemical shift correlation spectrum, where only the chemical shifts of those protons, which are bonded to two neighboring carbons, are correlated. By extracting 2D F1/F3 slices ({sup 1}H/{sup 1}H chemical shift correlation spectrum) at different {sup 13}C chemical shift frequencies from the 3D {sup 1}H/{sup 13}C/{sup 1}H spectrum, resonances of proton atoms located close to a specific carbon atom can be identified. Overall, the through-bond and through-space homonuclear/heteronuclear proximities determined from the

  19. Systèmes complexes gouvernés par des flux : schémas de volumes finis hybrides et optimisation numérique

    Jaisson, Pascal

    2006-01-01

    Cette thèse concerne la modélisation par des EDP et la résolution numérique de problèmes d'optimisation pour les flux d'information et pour le trafic routier. Nous proposons un nouveau type de schémas hybrides. En premier, nous nous intéressons à l'optimisation des temps de service nécessaires à un serveur informatique pour traiter un ou plusieurs types de requêtes, afin de satisfaire une qualité de service imposée. La modélisation choisie fait intervenir un système de lois de conservation pr...

  20. Identification of Li-Ion Battery SEI Compounds through (7)Li and (13)C Solid-State MAS NMR Spectroscopy and MALDI-TOF Mass Spectrometry.

    Huff, Laura A; Tavassol, Hadi; Esbenshade, Jennifer L; Xing, Wenting; Chiang, Yet-Ming; Gewirth, Andrew A

    2016-01-13

    Solid-state (7)Li and (13)C MAS NMR spectra of cycled graphitic Li-ion anodes demonstrate SEI compound formation upon lithiation that is followed by changes in the SEI upon delithiation. Solid-state (13)C DPMAS NMR shows changes in peaks associated with organic solvent compounds (ethylene carbonate and dimethyl carbonate, EC/DMC) upon electrochemical cycling due to the formation of and subsequent changes in the SEI compounds. Solid-state (13)C NMR spin-lattice (T1) relaxation time measurements of lithiated Li-ion anodes and reference poly(ethylene oxide) (PEO) powders, along with MALDI-TOF mass spectrometry results, indicate that large-molecular-weight polymers are formed in the SEI layers of the discharged anodes. MALDI-TOF MS and NMR spectroscopy results additionally indicate that delithiated anodes exhibit a larger number of SEI products than is found in lithiated anodes. PMID:26653886

  1. Corrado Neri, Âges inquiets. Cinémas chinois : une représentation de la jeunesse

    Pernin, Judith

    2010-01-01

    Corrado Neri, Âges inquiets. Cinémas chinois : une représentation de la jeunesse, Lyon, Tigre de papier, 2009, 533 p. La jeunesse dans le « cinéma de langue chinoise » est le sujet de cet ouvrage de Corrado Neri, publié à partir de sa thèse de doctorat. Il se consacre à une analyse à la fois historique et trans-territoriale de la représentation de la jeunesse dans le cinéma de Taiwan, de Hong Kong et de la République populaire de Chine. Par ce terme de jeunesse, qui peut paraître vague et cul...

  2. El sueño de Venecia o el guiño de los clásicos en la narrativa de Paloma Díaz-Mas

    Neus Samblancat

    2010-06-01

    Full Text Available Su artículo propone que la concepción de la obra de Paloma Díaz-Mas, El sueño de Venecia, como palimpsesto literario nos conduce a un análisis intertextual donde cada capítulo de la novela nos remite a un género y a unas voces de época. Los clásicos, como el resto de la producción narrativa de la autora, guiñan el ojo a lo largo de una obra que plantea una reflexión sobre la fragilidad de la razón y de la humana Memoria a la hora de conocer la Historia y de explicar una historia.

  3. Solid state P-31 MAS NMR spectroscopy and conductivity measurements on NbOPO4 and H3PO4 composite materials

    Risskov Sørensen, Daniel; Nielsen, U. G.; Skou, E. M.

    2014-01-01

    .1 and 74.2 M% were produced and characterized with powder X-ray diffraction, P-31 MAS NMR and impedance spectroscopy. NMR revealed that a significant degree of dehydration and vaporization of H3PO4 takes place above 200 degrees C, and increases with temperature. At 500 degrees C the NbOPO4 and H3PO4 has...... reacted to form niobium pyrophosphate (Nb2P4O15). Impedance spectroscopy showed an increase in conductivity with increasing acid concentration, whereas the conductivity decreased slightly with increasing temperature. The highest conductivity measured was 2.5.10(-3) S/cm for a sample containing 74.2 M% of...

  4. Solid state NMR of proteins at high MAS frequencies: symmetry-based mixing and simultaneous acquisition of chemical shift correlation spectra

    Bellstedt, Peter [Fritz Lipmann Institute, Biomolecular NMR spectroscopy, Leibniz Institute for Age Research (Germany); Herbst, Christian [Ubon Ratchathani University, Department of Physics, Faculty of Science (Thailand); Haefner, Sabine; Leppert, Joerg; Goerlach, Matthias; Ramachandran, Ramadurai, E-mail: raman@fli-leibniz.de [Fritz Lipmann Institute, Biomolecular NMR spectroscopy, Leibniz Institute for Age Research (Germany)

    2012-12-15

    We have carried out chemical shift correlation experiments with symmetry-based mixing sequences at high MAS frequencies and examined different strategies to simultaneously acquire 3D correlation spectra that are commonly required in the structural studies of proteins. The potential of numerically optimised symmetry-based mixing sequences and the simultaneous recording of chemical shift correlation spectra such as: 3D NCAC and 3D NHH with dual receivers, 3D NC Prime C and 3D C Prime NCA with sequential {sup 13}C acquisitions, 3D NHH and 3D NC Prime H with sequential {sup 1}H acquisitions and 3D CANH and 3D C'NH with broadband {sup 13}C-{sup 15}N mixing are demonstrated using microcrystalline samples of the {beta}1 immunoglobulin binding domain of protein G (GB1) and the chicken {alpha}-spectrin SH3 domain.

  5. Agent Virus of Cooperation (Avicoop: an Intelligent Model of Cooperation and Collaboration Based on the MAS for Data Exchange in Ad hoc Network

    Claude Tangha

    2012-09-01

    Full Text Available We propose in this paper a response to the problem of cooperation and collaboration between nodes in a mobile ad hoc network (MANET for intelligent data relays from one source to a destination. In their operation, each routing protocol relies on the support of each node for end-to-end messages routing by completely ignoring the autonomous nature of the node that is to be free to accept, reject, relay or otherwise simply manipulate the protocols principle. This random and unpredictable behavior of the node impacts on the routing effectiveness in the global network. Our approach is based on multi-agent systems (MAS and game theory, especially the iterated version of the Prisoner's Dilemma. The proposed model is validated by a prototype.

  6. Solid state CP/MAS 13C n.m.r. analysis of particle size and density fractions of soil incubated with uniformly labelled 13C-glucose

    A soil incubated for 34 days in the absence (control) and presence (treated) of uniformly labelled 13C-glucose was dispersed using an ultrasonic probe and fractionated by sedimentation in water and a polytungstate solution of density 2.0 Mg m-3. Solid state CP/MAS 13C n.m.r. (cross polarization/magic angle spinning 13C nuclear magnetic resonance) spectroscopy was used to characterize the chemical structure of the native soil organic carbon and the residual substrate carbon in the fractions of the control and treated soils. To obtain quantitative results it was essential to determine the spin lattice relaxation time in a rotating frame of the individual carbon types in the spectra as the relaxation behaviour of the native organic material in the clay fraction was different from that of the residual substrate carbon. The residual substrate carbon was found to accumulate in predominantly alkyl and O-alkyl structures in both fractions. However, significant amounts of acetal and carboxyl carbon were also observed in the clay fraction. Little if any aromatic or phenolic carbon was synthesized by the soil microorganisms utilizing substrate carbon. Dipolar dephasing CP/MAS 13C n.m.r. experiments were also performed and allowed the proportion of each type of carbon which was protonated and nonprotonated to be estimated. Essentially all of the O-alkyl and acetal carbon, 25-40% of the aromatic carbon and 66-80% of the alkyl carbon was protonated in the fractions isolated from the treated soil. 24 refs., 4 figs., 2 tabs

  7. Proton-detected 3D 1H/13C/1H correlation experiment for structural analysis in rigid solids under ultrafast-MAS above 60 kHz

    Zhang, Rongchun; Nishiyama, Yusuke; Ramamoorthy, Ayyalusamy

    2015-10-01

    A proton-detected 3D 1H/13C/1H chemical shift correlation experiment is proposed for the assignment of chemical shift resonances, identification of 13C-1H connectivities, and proximities of 13C-1H and 1H-1H nuclei under ultrafast magic-angle-spinning (ultrafast-MAS) conditions. Ultrafast-MAS is used to suppress all anisotropic interactions including 1H-1H dipolar couplings, while the finite-pulse radio frequency driven dipolar recoupling (fp-RFDR) pulse sequence is used to recouple dipolar couplings among protons and the insensitive nuclei enhanced by polarization transfer technique is used to transfer magnetization between heteronuclear spins. The 3D experiment eliminates signals from non-carbon-bonded protons and non-proton-bonded carbons to enhance spectral resolution. The 2D (F1/F3) 1H/1H and 2D 13C/1H (F2/F3) chemical shift correlation spectra extracted from the 3D spectrum enable the identification of 1H-1H proximity and 13C-1H connectivity. In addition, the 2D (F1/F2) 1H/13C chemical shift correlation spectrum, incorporated with proton magnetization exchange via the fp-RFDR recoupling of 1H-1H dipolar couplings, enables the measurement of proximities between 13C and even the remote non-carbon-bonded protons. The 3D experiment also gives three-spin proximities of 1H-1H-13C chains. Experimental results obtained from powder samples of L-alanine and L-histidine ṡ H2O ṡ HCl demonstrate the efficiency of the 3D experiment.

  8. Saules kombinētās siltumenerģētiskās sistēmas darbības algortima un simulācijas modeļa izstrāde

    Rochas, C.; Jaunzems, D

    2006-01-01

    Darbā var iepazīties ar jaunu saules kombinētās siltumenerģētiskās sistēmas prototipu – tā uzbūvi, darbības režīmiem, kā arī kontroles un vadības stratēģijas aprakstu. Paralēli ir izstrādāts jaunā sistēmas prototipa darbības un vadības algoritms, kas izmantots sistēmas simulācijas modeļa izveidē un pārbaudīts simulāciju programmas TRNSYS vidē.

  9. Comparative HPLC-DAD-ESI(+MS Fingerprint and Quantification of Phenolic and Flavonoid Composition of Aqueous Leaf Extracts of Cornus mas and Crataegus monogyna, in Relation to Their Cardiotonic Potential

    Marius BADALICA-PETRESCU

    2014-06-01

    Full Text Available Our study aimed to compare the phenolic and flavonoid composition of two medicinal plants from the wild flora of Romania, traditionally known to be efficient in preventing cardiotoxicity: Cornus mas and Crataegus monogyna. As most previous studies have focused on fruits, our investigations aimed to fingerprint and quantify the two classes of compounds in aqueous leaf extracts, based on HPLC-DAD-ESI(+MS analysis. The specific HPLC-DAD fingerprint was coupled with UV spectra at 280 and 340 nm to discriminate between three subclasses of compounds: two hydroxycinnamic acids and a flavonoid glycoside. While the C. monogyna extract contained more than 98% vitexin isomers (2’- and 4’-O-rhamnoside, the C. mas extract was very complex, containing a mixture of phenolic derivatives and flavonoid glycosides. The spectral patterns of C. mas, combined with the molecular mass and specific fragmentations allowed the identification of epi-catechin, coumaric and caffeic acids and quercetin derivatives. Based on LC-MS peak area and parallel calibrations with gallic acid and rutin, the mean concentration for flavonoids in C. mas was 13 mg/100 ml, while aprox. 65 mg/100 ml in C. monogyna extracts. Using the Folin method, the total phenol content was 105 mg/100 ml in C. mas and around three times less in the C. monogyna extract, while the antioxidant activity was increased only 1.5-1.7 times in C. mas extract compared to C. monogyna. These findings suggest a higher stability and potential of flavonoids to act as antioxidants in hydrophilic environment.

  10. MAS based energy management strategies of microgrid%基于Multi-Agent系统的含分布式电源电网能源优化管理

    李文云; 蒋亚坤; 雷炳银; 于峰

    2015-01-01

    在市场运行环境下,为了寻求微电网成本利益和环境效益的最大化,提出基于 Multi-Agent 系统能源优化管理策略。该研究首先构建Multi-Agent系统。此系统包括市场管理Agent (MOA),微电网管理Agent (MMA),公共电网Agent (UGA)和分布式发电Agent (DGA)。根据各微电网管理Agent上报的发电计划,在市场管理Agent中设计电价竞标策略用来确定各微电网的最佳交易电价和中标电量,以确保微电网经济利益最大化。在此基础上,在各微电网管理Agent中设计能量管理策略,并采用改进粒子群算法来确定微电网内部各分布式发电Agents的最佳功率分派,从而最小化微电网的运行成本。最后仿真验证了该方案的有效性。%A Multi-Agent System (MAS) based energy optimization management strategy for microgrid in market operation environment is proposed in order to maximize the cost and environment benefit. Firstly, the MAS is constructed, which consists of one market operator Agent (MOA), several MG Management Agents (MMA), one Utility Grid Agent (UGA), and many DG Agents (DGA). Then the power price bidding strategy is designed in the MOA according to the generation power plan provided by all the MMAs. By the power price bidding strategy, the best trade price and bid-winning power are determined in order to maximize economic benefit. On the basis of this, the energy management strategy is designed in MMAs, by which the optimal power dispatches for all DGAs are obtained by using an improved particle swarm optimization method for minimizing the operating cost. Finally, the validity of proposed method is demonstrated by means of simulation results.

  11. Uzņēmuma SIA H&M HENNES&MAURITZ reklāmas satura analīze un pilnveidošanas iespējas.

    Jasinska, Liene

    2016-01-01

    Mūsdienu pasaule ir pilna ar reklāmām. Reklāmas ir redzamas visur – televīzijā, uz ielas, radio, žurnālos, internetā un citur. Blakus viena otrai atrodas pilnīgi vienādas preču kategorijas reklāma vai arī pilnīgi dažādas. Cilvēkam esot šajā reklāmu jūrā ir grūti uztvert visas reklāmas un to piedāvāto informāciju, bet vēl grūtāk ir uzņēmumiem, kuriem šajā reklāmas jūrā ir jāievieto sava reklāma tā, lai cilvēki to pamanītu un uztvertu. Uzņēmuma reklāmas veiksme slēpjas tās veiksmīgā uzbūvē un t...

  12. "McDonald's" reklāmas analīze Latvijā un Maltā - starpkultūru atšķirīgā uztvere

    Barkēviča, Santa

    2016-01-01

    Darbā ““McDonald's” reklāmas analīze Latvijā un Maltā – starpkultūru atšķirīgā uztvere” galvenais problēmjautājums, kas jānoskaidro, vai starptautisks uzņēmums var izmantot viena stila reklāmas kampaņu divās dažādās valstīs – Latvijā un Maltā. Galvenais darba mērķis ir noskaidrot, vai starptautisks uzņēmums var veidot vienu reklāmas kampaņu shēmu, un to izmantot dažādās valstīs, kur atrodas tā franšīzes vai filiāles, un kā starpkultūru atšķirība ietekmē reklāmas uztveri. Veicot aptaujas, inte...

  13. Investigation of the Structure and Active Sites of TiO2 Nanorod Supported VOx Catalysts by High-Field and Fast-Spinning 51V MAS NMR

    Hu, Jian Z.; Xu, Suochang; Li, Weizhen; Hu, Mary Y.; Deng, Xuchu; Dixon, David A.; Vasiliu, Monica; Craciun, Raluca; Wang, Yong; Bao, Xinhe; Peden, Charles HF

    2015-07-02

    Supported VOx/TiO2-Rod catalysts were studied by 51V MAS NMR at high field using a sample spinning rate of 55 kHz. The superior spectral resolution allows for the observation of at least five vanadate species. The assignment of these vanadate species was carried out by quantum mechanical calculations of 51V NMR chemical shifts of model V-surface structures. Methanol oxidative dehydrogenation (ODH) was used to establish the correlation between the reaction rate and the various surface V-sites. It is found that monomeric V-species dominated the catalyst at low vanadium loadings with two peaks observed at about -502 and -529 ppm. V-dimers with two bridged oxygen appeare at about -555 ppm. Vanadate dimers and polyvanadates connected by one bridged oxygen atom between two adjacent V atoms resonate at about -630 ppm. A positive correlation is found between the V-dimers related to the -555 ppm peak and the ODH rate while a better correlation is obtained by including monomeric contributions. This result indicates that surface V-dimers related to the -555 ppm peak are the major active sites for ODH reaction despite mono-V species are more catalytic active but their relative ratios are decreased dramatically at high V-loadings. Furthermore, a portion of the V-species is found invisible. In particular, the level of such invisibility increases with decreased level of V-loading, suggesting the existence of paramagnetic V-species at the surface.

  14. ATIVIDADE EPILINGUÍSTICA E O ENSINO DE LÍNGUA MATERNA: UM EXERCÍCIO COM A CONJUNÇÃO "MAS"

    Camila Arndt Wamser

    2013-06-01

    Full Text Available O texto discute a importância do trabalho com a atividade epilinguística, entendida como a própria atividade de linguagem, no ensino de língua materna. O conceito de atividade epilinguística que adotamos é o da Teoria das Operações Predicativas e Enunciativas, elaborada pelo linguista francês Antoine Culioli. Com o intuito de delimitar o objeto de pesquisa e apresentar um modelo de atividade aplicado em sala de aula, trabalhamos com a conjunção adversativa mas, concebendo-a como marcador léxico-gramatical. O exercício foi aplicado a uma turma do sétimo ano da Escola Estadual Dr. Joaquim Batista, na cidade de Jaboticabal/SP. Como resultado do trabalho com a atividade epilinguística, os alunos tiveram condições de observar os mais variados contextos em que a marca aparece, bem como, compreender que as palavras não apresentam um significado estático. Além disso, esse trabalho permitiu aos alunos que construíssem seu próprio conhecimento e elaborassem uma gramática mais operacional e relevante.

  15. An efficient method for solving the MAS stiff system of nonlinearly coupled equations: Application to the pseudoelastic response of shape memory alloys (SMA)

    Mourad, A.; Kamel, Z.

    2016-04-01

    A Shape memory alloy (SMA) actuators have great potential in advanced technology applications where space, weight and cost are crucial design factors. They are known for the shape memory effect which is the ability to recover an initial configuration by simple heating after deformation. SMAs also exhibit a behavior called "pseudoelasticity" also known as "superelasticity" which is the shape recovery associated with mechanical loading and unloading at temperatures above specific values. The key characteristic of SMAs is the martensitic phase transformation, brought about by temperature change and/or by application of stress. Martensitic transformation is usually accompanied by significant changes in mechanical, electrical and thermal properties that render them as prime candidates for the development of smart structures and devices. This work is a contribution to the study of the influence of parameters such as operating temperature and the mode of heat transfer (natural or forced convection) on the pseudoelastic response of SMA used as actuators. Based on the mathematical formalism of the Müller, Achenbach and Seelecke model known as the "MAS model", we develop through a new mathematical formalism a new iterative resolution methodology of the coupled equations. The new approach provides very significant results and allows a significant gain in computation time.

  16. Türkiye’de İngilizce Öğreniminin Yaygınlaşmasının Nedenleri

    Emsal ATEŞ ÖZDEMİR

    2006-06-01

    Full Text Available Bu çalışmanın amacı Türkiye’de İngilizce öğreniminin yaygınlaşmasının sebepleriniincelemektir. Nedenler İngilizce öğretim yöntemleri ve kullanılan ders kitapları olarak belirtilmiştir.Bazı yöntemler ve ders kitapları aracılığı ile Türk insanlarına İngilizce’nin hayatın her aşamasındaçok önemli olduğu mesajı verilmektedir. Aslında bunun altında diğer gelişmekte olan ülkelerdeolduğu gibi Türkiye’de de yabancı kitapları satan iki ülkenin, İngiltere ve Amerika’nın maddiçıkarları yatmaktadır. İngilizce’nin bilgiye ulaşmak ve dünyadaki diğer insanlarla iletişim kurmak içinhayati önem taşıdığı inkar edilemez ancak kişi İngilizce öğrenme nedenleri konusunda bilinçliolmalıdır.

  17. Asetaminofenin koaservasyon-faz ayrışması yöntemiyle mikrokapsüllerinin hazırlanması

    KARATAŞ, Ayşegül

    1989-01-01

     Bu çalışmada, kısa yarı ömürlü bir etken madde olan Asetaminafen'in kaaservasyan-faz ayrışması yöntemlerinden biri olan ortama polimeri çözmeyen sıvı (non-salvent) ilavesi metodu kullanılarak uzatılmış etkili mikrokapsüllerin hazırlanması amaçlanmıştır. Bu amaçla, etken maddenin 88-177 um, 250-35^ um ve 420-500 pm olan üç farklı partikül büyüklüğü aralığı kullanılmıştır. Non-solvent olarak % 6 a/a poliisobutilen'in siklohekzan içersindeki 60 gr çözeltisi D. 9 g. dak '...

  18. Amino-acid selective experiments on uniformly 13C and 15N labeled proteins by MAS NMR: Filtering of lysines and arginines

    Jehle, Stefan; Rehbein, Kristina; Diehl, Anne; van Rossum, Barth-Jan

    2006-12-01

    Amino-acid selective magic-angle spinning (MAS) NMR experiments can aid the assignment of ambiguous cross-peaks in crowded spectra of solid proteins. In particular for larger proteins, data analysis can be hindered by severe resonance overlap. In such cases, filtering techniques may provide a good alternative to site-specific spin-labeling to obtain unambiguous assignments that can serve as starting points in the assignment procedure. In this paper we present a simple pulse sequence that allows selective excitation of arginine and lysine residues. To achieve this, we make use of a combination of specific cross-polarization for selective excitation [M. Baldus, A.T. Petkova, J. Herzfeld, R.G. Griffin, Cross polarization in the tilted frame: assignment and spectral simplification in heteronuclear spin systems, Mol. Phys. 95 (1998) 1197-1207.] and spin diffusion for transfer along the amino-acid side-chain. The selectivity of the filter is demonstrated with the excitation of lysine and arginine side-chain resonances in a uniformly 13C and 15N labeled protein preparation of the α-spectrin SH3 domain. It is shown that the filter can be applied as a building block in a 13C- 13C lysine-only correlation experiment.

  19. Effect of organic matter application on CP-MAS-13C-NMR spectra of humic acids from a brown soil

    Dou, S.

    2009-04-01

    The humified SOM or humic substances (HS) composed of humic acid (HA), fulvic acid (FA) and humin (HM) represent the most microbially recalcitrant and stable reservoir of organic carbon in soil (Piccolo et al., 2004). OM applications can influence the amount and structural characteristics of HS(Dou et al., 2008). During the past few decades, there has been much research on HS, but their chemical structure is still not fully understood (Dong, 2006).CP-MAS-13C-NMR spectroscopy was considered as an effective method to study structures of HS without dissolving problem compared with liquid 13C-NMR (Conte et al., 1997; Dou et al., 2008). It can directly measure the carbon framework and reflect the nature of HS transformation after OM application (Spaccini et al., 2000). For that reason, this method was applied in this study. The objective of this paper was to clarify the effect of long term OM application on the changes of structural characteristics in HAs, which provided new information for improving soil fertility by OM application. The experiment was carried out on a brown soil (Paleudalf in USDA Soil Taxonomy) at Shenyang Agricultural University, Liaoning province, China (N41°48'-E123°25'). The experiment included 3 treatments: zero-treatment (CKbr), and two pig manure (PM) treatments (O1 and O2) at the rates of 0.9 t ha-1 and 1.8 t ha-1 of organic carbon, respectively. The samples of the HA fraction were extracted, separated and purified according to the method described by Dou et al. (1991). Elemental composition, Differential thermal analysis (DTA), -lgK value, FT-IR and CP-MAS- 13C-NMR of HAs were performed. Effects on the contents of orgaic carbon and its composition. The contents of TOC were from 8.77 g kg-1 to 12.25 g kg-1. The relative contents in TOC for WSS, HA, and FA were 6.87%, 14.2% and 19.8%. Comparing the CKbr, the contents of WSS, HA and FA for O1 and O2 increased, but relative contents of WSS and FA decreased. The content of the HA increased after

  20. High Tech M&As

    Toppenberg, Gustav

    2013-01-01

    Technology driven industries have seen fast moving technology changes, higher complexity and reduced product life cycles. These emerging trends present challenges for companies in industries where technology is at the forefront. The extant research deals with ‘low-tech’ industries and majority of......&A teams in this sector. The phenomenon is studied from multiple perspectives: integration team, acquiring group and the company being acquired....

  1. Mas Roig Mini-Grid

    Salas, Pep; Guerrero, Josep M.

    This paper presents a microgrid project, named Basic Distributed Nodes (NoBaDis), deployed in a stand-alone farm in Girona, Spain. NoBaDis is based on intensive use of information to maximize the penetration of renewable energy through two main strategies: anticipation and opportunity. The loads ...

  2. Robotic Helicopter Surveying (MAS 13)

    Srikanth Saripalli; Jonathan Kelly; Sukhatme, Gaurav S.

    2006-01-01

    We present a method for highly accurate surveying using an autonomous helicopter equipped with a downward looking camera. Given a location that needs to be mapped the helicopter creates accurate aerial maps by combining visual data with inertial and GPS data. We present initial results that show maps of Lake Fulmor using the helicopter. These maps were created manually but they demonstrate the method used.

  3. Miroslav Masák

    Švácha, Rostislav

    [Řevnice]: Arbor vitae, 2012 - (Švácha, R.), s. 244-247 ISBN 978-80-87164-87-7 Institutional support: RVO:68378033 Keywords : architecture * postwar architecture * Liberec Subject RIV: AL - Art, Architecture, Cultural Heritage

  4. Miroslav Masák

    Švácha, Rostislav

    Praha: Arbor vitae, 2010 - (Švácha, R.), s. 292-299 ISBN 978-80-87164-41-9 Institutional research plan: CEZ:AV0Z80330511 Keywords : architecture * postwar architecture * Liberec Subject RIV: AL - Art, Architecture, Cultural Heritage

  5. Analysis of local conformation of membrane-bound and polycrystalline peptides by two-dimensional slow-spinning rotor-synchronized MAS exchange spectroscopy

    Gabrys, Charles M.; Yang Jun; Weliky, David P. [Michigan State University, Department of Chemistry (United States)], E-mail: weliky@cem.msu.edu

    2003-05-15

    2D slow-spinning, rotor-synchronized MAS exchange spectroscopy (SSRS-MASE) was applied to study local secondary structure of three structurally different peptides, two of which were membrane-bound. Each peptide was {sup 13}C carbonyl labeled at two adjacent residues in the peptide backbone. In general, this methodology is attractive for membrane-bound peptides because of its lenient spinning, decoupling, and RF homogeneity requirements.For a single set of raw SSRS-MASE data, two linearly independent methods exist for obtaining a 2D spectrum and each spectrum can be fit to obtain conformational constraints. An approach is described for combining the results of these two fits and this method is shown to work for spectra with both resolved and unresolved labeled site resonances. A spectrum is often fit well to a few different conformations which have somewhat different values of the fitting parameter {chi}{sup 2}. A simple statistical theory is developed which relates the {delta}{chi}{sup 2} difference between a local minimum and the global minimum {chi}{sup 2} to the likelihood that the local minimum conformation is the correct structure. Because uncertainty in the simulated data can also contribute to the overall fitting uncertainty, an empirical method is described for incorporating the simulation uncertainty into the {delta}{chi}{sup 2} analysis.These data analysis methods were tested on polycrystalline Ala-Gly-Gly and then applied to the membrane-bound melittin and HIV-1 fusion peptides. Melittin gave a best-fit {alpha} helical structure at Ala-4 while the fusion peptide gave a good-fit {beta} strand structure at Phe-8. The melittin analysis is in agreement with the known overall structure of this peptide.

  6. Analysis of mercerization process based on the intensity change of deconvoluted resonances of 13C CP/MAS NMR: Cellulose mercerized under cooling and non-cooling conditions

    The area intensity change of C1, C4, and C6 in spectrum obtained by 13C CP/MAS NMR and the mutual relationship between their changes were examined for cellulose samples treated with various concentrations of aqueous NaOH solutions under non-cooling and cooling conditions. The area intensity of C1-up and C6-down changed cooperatively with that of C4-down which corresponds to the crystallinity of samples: “-up” and “-down” are the up- and down- field component in a splitting peak of NMR spectrum, respectively. The intensity change of C1-up starts to decrease with decreasing in that of C4-down after that of C6-down is almost complete. These changes were more clearly observed for samples treated under cooling condition. It can be suggested that their characteristic change relates closely to the change in conformation of cellulose chains by induced decrystallization and the subsequent crystallization of cellulose II, and presumed that their changes at microscopic level relate to the macroscopic morphological changes such as contraction along the length of cellulose chains and recovery along the length. - Highlight: • Samples were mercerized at various NaOH concentrations under non-cooling and cooling. • The intensity change of C1 starts immediately after that of C6 is complete. • The creation of cell-II starts when decrystallization proceeds to a certain state. • This change relates closely to the change in conformation of cellulose chains. • The above change is more clearly found for samples treated under cooling

  7. Reversal of neurochemical alterations in the spinal dorsal horn and dorsal root ganglia by Mas-related gene (Mrg) receptors in a rat model of spinal nerve injury.

    Wang, Dongmei; Xue, Yaping; Yan, Yanhua; Lin, Minjie; Yang, Jiajia; Huang, Jianzhong; Hong, Yanguo

    2016-07-01

    The rodent Mas-related gene (Mrg) receptor subtype C has been demonstrated to inhibit pathological pain. This study investigated the mechanisms underlying the reversal of pain hypersensitivity by the selective MrgC receptor agonist bovine adrenal medulla 8-22 (BAM8-22) in a rat model of L5 spinal nerve ligation (SNL). Intrathecal (i.t.) administration of BAM8-22 (0.1-10nmol) attenuated mechanical allodynia in a dose-dependent manner on day 10 after SNL. The antiallodynia effect of BAM8-22 was abolished by MrgC receptor antibody, but not by naloxone. I.t. BAM8-22 (10nmol) inhibited SNL-induced upregulation of neuronal nitric oxide synthesis (nNOS) and phosphorylation of cyclic AMP response element-binding protein (p-CREB) in the spinal dorsal horn. The BAM8-22 treatment reversed the SNL-induced astrocyte activation, increase of interleukin-1β (IL-1β) expression and phosphorylation of extracellular signal-regulated kinase (p-ERK) in the spinal cord. BAM8-22 also reversed the upregulation of fractalkine and IL-1β in small- and medium-sized dorsal root ganglion (DRG) neurons. Furthermore, the BAM8-22 exposure suppressed the lipopolysaccharide (LPS)-induced increase of nNOS and IL-1β in the DRG explant cultures and the BAM8-22-induced suppression disappeared in the presence of MrgC receptor antibody. The present study provides evidence that activation of MrgC receptors inhibits nerve injury-induced increase of pronociceptive molecules in DRG neurons, suppressing astrocyte activation, the upregulation of excitatory mediators and phosphorylation of transcription factors in the spinal dorsal horn. As MrgC receptors are unequally expressed in the dorsal root and trigeminal ganglia, this study suggests that targeting MrgC receptors could be a new therapy for neuropathic pain with limited unwanted effects. PMID:27018398

  8. Assigning large proteins in the solid state: a MAS NMR resonance assignment strategy using selectively and extensively 13C-labelled proteins

    In recent years, solid-state magic-angle spinning nuclear magnetic resonance spectroscopy (MAS NMR) has been growing into an important technique to study the structure of membrane proteins, amyloid fibrils and other protein preparations which do not form crystals or are insoluble. Currently, a key bottleneck is the assignment process due to the absence of the resolving power of proton chemical shifts. Particularly for large proteins (approximately >150 residues) it is difficult to obtain a full set of resonance assignments. In order to address this problem, we present an assignment method based upon samples prepared using [1,3-13C]- and [2-13C]-glycerol as the sole carbon source in the bacterial growth medium (so-called selectively and extensively labelled protein). Such samples give rise to higher quality spectra than uniformly [13C]-labelled protein samples, and have previously been used to obtain long-range restraints for use in structure calculations. Our method exploits the characteristic cross-peak patterns observed for the different amino acid types in 13C-13C correlation and 3D NCACX and NCOCX spectra. An in-depth analysis of the patterns and how they can be used to aid assignment is presented, using spectra of the chicken α-spectrin SH3 domain (62 residues), αB-crystallin (175 residues) and outer membrane protein G (OmpG, 281 residues) as examples. Using this procedure, over 90% of the Cα, Cβ, C' and N resonances in the core domain of αB-crystallin and around 73% in the flanking domains could be assigned (excluding 24 residues at the extreme termini of the protein)

  9. Assigning large proteins in the solid state: a MAS NMR resonance assignment strategy using selectively and extensively {sup 13}C-labelled proteins

    Higman, Victoria A. [Leibniz-Institut fuer Molekulare Pharmakologie (Germany); Flinders, Jeremy [Genentech, Inc., Structural Biology Department (United States); Hiller, Matthias; Jehle, Stefan; Markovic, Stefan; Fiedler, Sebastian; Rossum, Barth-Jan van; Oschkinat, Hartmut [Leibniz-Institut fuer Molekulare Pharmakologie (Germany)], E-mail: oschkinat@fmp-berlin.de

    2009-08-15

    In recent years, solid-state magic-angle spinning nuclear magnetic resonance spectroscopy (MAS NMR) has been growing into an important technique to study the structure of membrane proteins, amyloid fibrils and other protein preparations which do not form crystals or are insoluble. Currently, a key bottleneck is the assignment process due to the absence of the resolving power of proton chemical shifts. Particularly for large proteins (approximately >150 residues) it is difficult to obtain a full set of resonance assignments. In order to address this problem, we present an assignment method based upon samples prepared using [1,3-{sup 13}C]- and [2-{sup 13}C]-glycerol as the sole carbon source in the bacterial growth medium (so-called selectively and extensively labelled protein). Such samples give rise to higher quality spectra than uniformly [{sup 13}C]-labelled protein samples, and have previously been used to obtain long-range restraints for use in structure calculations. Our method exploits the characteristic cross-peak patterns observed for the different amino acid types in {sup 13}C-{sup 13}C correlation and 3D NCACX and NCOCX spectra. An in-depth analysis of the patterns and how they can be used to aid assignment is presented, using spectra of the chicken {alpha}-spectrin SH3 domain (62 residues), {alpha}B-crystallin (175 residues) and outer membrane protein G (OmpG, 281 residues) as examples. Using this procedure, over 90% of the C{alpha}, C{beta}, C' and N resonances in the core domain of {alpha}B-crystallin and around 73% in the flanking domains could be assigned (excluding 24 residues at the extreme termini of the protein)

  10. Analysis of local conformation of membrane-bound and polycrystalline peptides by two-dimensional slow-spinning rotor-synchronized MAS exchange spectroscopy

    2D slow-spinning, rotor-synchronized MAS exchange spectroscopy (SSRS-MASE) was applied to study local secondary structure of three structurally different peptides, two of which were membrane-bound. Each peptide was 13C carbonyl labeled at two adjacent residues in the peptide backbone. In general, this methodology is attractive for membrane-bound peptides because of its lenient spinning, decoupling, and RF homogeneity requirements.For a single set of raw SSRS-MASE data, two linearly independent methods exist for obtaining a 2D spectrum and each spectrum can be fit to obtain conformational constraints. An approach is described for combining the results of these two fits and this method is shown to work for spectra with both resolved and unresolved labeled site resonances. A spectrum is often fit well to a few different conformations which have somewhat different values of the fitting parameter χ2. A simple statistical theory is developed which relates the Δχ2 difference between a local minimum and the global minimum χ2 to the likelihood that the local minimum conformation is the correct structure. Because uncertainty in the simulated data can also contribute to the overall fitting uncertainty, an empirical method is described for incorporating the simulation uncertainty into the Δχ2 analysis.These data analysis methods were tested on polycrystalline Ala-Gly-Gly and then applied to the membrane-bound melittin and HIV-1 fusion peptides. Melittin gave a best-fit α helical structure at Ala-4 while the fusion peptide gave a good-fit β strand structure at Phe-8. The melittin analysis is in agreement with the known overall structure of this peptide

  11. Storage phosphor digital radiography in portable chest imaging: comparison of image quality with conventional film-screen system with variation of mAs

    To compare image quality of storage phosphor digital radiography (DR) with film-screen radiography in portable chest imaging, and to assess the minimum X-ray dose that can be applied to DR in adults without image degradation, and also to compare image qualities of low dose and standard dose DR. A geometrical phantom similar to the human thorax was imaged by a portable radiographic unit with fixed kVp and variable mAs in both film-screen and DR systems. Three radiologists scored the images by four grades in four categories of 1) contrast between mediastinum and lung, 2) definition of the nodule in the lung, 3) definition of another nodule through the mediastinal shadow, and 4) grainess (noise: assessed only in DR). Additionally, portable chest images were obtained in 10 patients in a intensive care unit by film-screen, standard dose and half dose DR in consecutive days. The same readers scored the images by four grades in six categories of 1) the lungs and hila, 2)the mediastinum, 3) subphrenic area. 4) musculoskeletal shadow, 5) tubes and lines, and 6) grainess (only in DR). The images with superior quality were assessed by paired t-test. In phantom study, the minimum dose of digital images scored 3 or more by all readers was 39% of the standard dose. In patient study, DR was superior to film-screen radiography in all categories except tube and line. Low dose DR was not inferior to standard dose DR in five categories other than grainess to two readers or more. In portable chest imaging, storage phosphor DR image was superior to conventional film-screen radiography and half dose DR was comparable to standard dose DR despite of more noise

  12. L-methionine decarboxylase from Dryopteris filix-mas: Purification, characterization, substrate specificity, abortive transamination of the coenzyme, and stereochemical courses of substrate decarboxylation and coenzyme transamination

    L-Methionine decarboxylase from the male fern Dryopteris filix-mas has been purified 256-fold from acetone powder extracts to very near homogeneity. The enzyme is membrane-associated and requires detergent for solubilization during the initial extraction. The enzyme is a homodimer of subunit Mr 57,000 and shows a pH optimum at ∼ 5.0 with 20 mM (2S)-methionine as substrate. A wide range of straight- and branched-chain (2S)-alkylamino acids are substrates for the enzyme. The values for the rate of decarboxylation, Vmax, and for the apparent Michaelis constant, Km, however, vary with structure and with the chirality at C-3. The pH dependence of V and V/K has been examined for three substrates: (2S)-methionine, valine, and leucine. The occurrence of the abortive reaction was confirmed by showing that [35S]methionine is converted to labeled 3-(methylthio)propionaldehyde while [4'-3H]PLP is converted to labeled pyridoxamine 5'-phosphate (PMP). The decarboxylation of (2S)-methionine gave 3(methylthio)-1-aminopropane. Preparation of the N-camphanamide derivative of the amine allowed the C-1 methylene protons to be distinguished by 1H NMR spectroscopy. Synthetic samples of the camphanamide were prepared in which each of the C-1 methylene protons was replaced by deuterium. When tritiated pyridoxal phosphate was incubated with the enzyme, tritiated pyridoxamine phosphate was formed. These results are used to construct possible mechanistic schemes for both reactions, decarboxylation and transamination. The position and possible identities of active-site proton donors are discussed

  13. Out-and-back {sup 13}C-{sup 13}C scalar transfers in protein resonance assignment by proton-detected solid-state NMR under ultra-fast MAS

    Barbet-Massin, Emeline; Pell, Andrew J. [University of Lyon, CNRS/ENS Lyon/UCB Lyon 1, Centre de RMN a Tres Hauts Champs (France); Jaudzems, Kristaps [Latvian Institute of Organic Synthesis (Latvia); Franks, W. Trent; Retel, Joren S. [Leibniz-Institut fuer Molekulare Pharmakologie (Germany); Kotelovica, Svetlana; Akopjana, Inara; Tars, Kaspars [Biomedical Research and Study Center (Latvia); Emsley, Lyndon [University of Lyon, CNRS/ENS Lyon/UCB Lyon 1, Centre de RMN a Tres Hauts Champs (France); Oschkinat, Hartmut [Leibniz-Institut fuer Molekulare Pharmakologie (Germany); Lesage, Anne; Pintacuda, Guido, E-mail: guido.pintacuda@ens-lyon.fr [University of Lyon, CNRS/ENS Lyon/UCB Lyon 1, Centre de RMN a Tres Hauts Champs (France)

    2013-08-15

    We present here {sup 1}H-detected triple-resonance H/N/C experiments that incorporate CO-CA and CA-CB out-and-back scalar-transfer blocks optimized for robust resonance assignment in biosolids under ultra-fast magic-angle spinning (MAS). The first experiment, (H)(CO)CA(CO)NH, yields {sup 1}H-detected inter-residue correlations, in which we record the chemical shifts of the CA spins in the first indirect dimension while during the scalar-transfer delays the coherences are present only on the longer-lived CO spins. The second experiment, (H)(CA)CB(CA)NH, correlates the side-chain CB chemical shifts with the NH of the same residue. These high sensitivity experiments are demonstrated on both fully-protonated and 100 %-H{sup N} back-protonated perdeuterated microcrystalline samples of Acinetobacter phage 205 (AP205) capsids at 60 kHz MAS.

  14. Proton-detected 3D 14N/14N/1H isotropic shift correlation experiment mediated through 1H-1H RFDR mixing on a natural abundant sample under ultrafast MAS

    Pandey, Manoj Kumar; Nishiyama, Yusuke

    2015-09-01

    In this contribution, we have demonstrated a proton detection-based approach on a natural abundant powdered L-Histidine HCl-H2O sample at ultrafast magic angle spinning (MAS) to accomplish 14N/14N correlation from a 3D 14N/14N/1H isotropic shift correlation experiment mediated through 1H finite-pulse radio frequency-driven recoupling (fp-RFDR). Herein the heteronuclear magnetization transfer between 14N and 1H has been achieved by HMQC experiment, whereas 14N/14N correlation is attained through enhanced 1H-1H spin diffusion process due to 1H-1H dipolar recoupling during the RFDR mixing. While the use of ultrafast MAS (90 kHz) provides sensitivity enhancement through increased 1H transverse relaxation time (T2), the use of micro-coil probe which can withstand strong 14N radio frequency (RF) fields further improves the sensitivity per unit sample volume.

  15. {sup 1}H and {sup 23}Na MAS NMR spectroscopy of cationic species in CO{sub 2} selective alkaline earth metal porous silicoaluminophosphates prepared via liquid and solid state ion exchange

    Arevalo-Hidalgo, Ana G. [Department of Chemical Engineering, University of Puerto Rico-Mayagueez Campus, Mayagueez, PR 00681-9000 (Puerto Rico); Dugar, Sneha; Fu, Riqiang [National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL 32310 (United States); Hernandez-Maldonado, Arturo J., E-mail: arturoj.hernandez@upr.edu [Department of Chemical Engineering, University of Puerto Rico-Mayagueez Campus, Mayagueez, PR 00681-9000 (Puerto Rico)

    2012-07-15

    The location of extraframework cations in Sr{sup 2+} and Ba{sup 2+} ion-exchanged SAPO-34 was estimated by means of {sup 1}H and {sup 23}Na MAS NMR spectroscopy and spectral deconvolution. Incorporation of the alkaline earth metal cations onto the SAPO framework was achieved via liquid state ion exchange, coupled partial detemplation/solid-state ion exchange, and combination of both techniques. MAS NMR revealed that the level of ion exchange was limited by the presence of protons and sodium cations near hexagonal prisms (site SI), which are relatively difficult to exchange with the alkaline earth metal due to steric and charge repulsion criteria. In addition, the presence of ammonium cations in the supercages facilitated the exchange of otherwise tenacious hydrogen as corroborated by unit cell compositional data as well as enhanced CO{sub 2} adsorption at low partial pressures. The extraframework ammonium species were produced from partial detemplation of the structure-directing agent employed for the SAPO-34 synthesis, tetraethylammonium. - Graphical abstract: MAS NMR was used to elucidate the position the cationic species in alkaline earth metal exchanged silicoaluminophosphates. These species played a significant role during the ion exchange process and, therefore, the materials ultimate CO{sub 2} adsorption performance. Highlights: Black-Right-Pointing-Pointer Location of extraframework Sr{sup 2+} or Ba{sup 2+} cations was estimated by means of {sup 1}H and {sup 23}Na MAS NMR. Black-Right-Pointing-Pointer Level of Sr{sup 2+} or Ba{sup 2+} ion exchange was limited by the presence of protons and sodium cations. Black-Right-Pointing-Pointer Presence of ammonium cations in the supercages facilitated the exchange. Black-Right-Pointing-Pointer Sr{sup 2+} and Ba{sup 2+} ion exchanged SAPOs are outstanding CO{sub 2} adsorbents.

  16. Comparative HPLC-DAD-ESI(+)MS Fingerprint and Quantification of Phenolic and Flavonoid Composition of Aqueous Leaf Extracts of Cornus mas and Crataegus monogyna, in Relation to Their Cardiotonic Potential

    Marius BADALICA-PETRESCU; DRAGAN, SIMONA; Floricuţa RANGA; Florinela FETEA; Socaciu, Carmen

    2014-01-01

    Our study aimed to compare the phenolic and flavonoid composition of two medicinal plants from the wild flora of Romania, traditionally known to be efficient in preventing cardiotoxicity: Cornus mas and Crataegus monogyna. As most previous studies have focused on fruits, our investigations aimed to fingerprint and quantify the two classes of compounds in aqueous leaf extracts, based on HPLC-DAD-ESI(+)MS analysis. The specific HPLC-DAD fingerprint was coupled with UV spectra at 280 and 340 nm ...

  17. Islamiskās finanses kā alternatīva tradicionālās finanšu sistēmas ētikas un morāles nepilnībām.

    Souissi, Intissar

    2016-01-01

    Starptautiskā finanšu sistēma šobrīd saskaras ar pārmērīgu parādu un globālo finanšu inženieriju. Islāma banku sistēmaatšķiras no parastās sistēmas, jo tā izturēja 2008. Gada lielo krīzi. Tas radīja lielu ieinteresētību ekspertos un pētniekos, jo tie vēlējās saprast, kas radīja tādu imunitāti islāma bankai. Arī šajā darbā tika pētīts un analizēts vai Islāma banka varētubūt potenciālais risinājums krīzei un patreizējās sistēmas reāla alternatīva. Darbā pēc globālās monetārās sistēmas apskata...

  18. BN interphase in composite materials with nicalon Si-C-O fibers and with vitro ceramic matrix of MAS type; L`interphase BN dans les materiaux composites a fibres Si-C-O nicalon et a matrice vitroceramique de type MAS

    Ricca, N.

    1994-03-14

    BN has been suggested as an interphase in silica-based glass-ceramic matrix composites with a view to use these materials in oxidizing atmospheres at medium or high temperatures. The matrix had a boron-doped MAS (MgO-Al{sub 2}O{sub 3}-SiO{sub 2}) composition and was prepared from an hydrosol precursor. Pseudo-ID composites were prepared according to a sol impregnations/calcination/hot-pressing route. Chemical and microstructural characterizations of the fiber/matrix interfacial area were conducted by mean of TEM/EELS and AES analyses. The efficiency of BN as a coupling interphase for this particular composite system was successfully demonstrated through tensile tests performed on either as-processed or aged specimens (100 hours at 1000 deg C in air or under argon). In addition, composites maintained in air at 600 deg C, 800 deg C and 900 deg C while simultaneously loaded did not fail after 150 hours or more. Thus, a BN interphase appeared to be compatible with an oxidizing environment (i.e. the oxide matrix and/or air from 600 to 1000 deg C) and should therefore successfully replace the usual carbon interphase at least for use at medium temperatures. (author)

  19. Phosphole complexes of Gold(I) halides: Comparison of solution and solid-state structures by a combination of solution and CP/MAS 31P NMR spectroscopy and x-ray crystallography

    A series of complexes of 1-phenyldibenzophosphole (DBP), 1-phenyl-3,4,-dimethylphosphole (DMPP), and triphenylphosphine of the type LnAuX (n = 1, L = DBP, DMPP, Ph3P, X = Cl, Br, I; n = 3, L = DBP, X = Cl, Br, I; n = 3, L = Ph3P, X = Cl; n = 4, L = DBP, DMPP, X = PF6) have been prepared and characterized. The structures of (DBP)AuCl (1), (DBP)3AuCl (2), and (DMPP)AuCl (3) have been determined from three-dimensional x-ray data collected by counter methods. Crystal structure of the complexes is reported. The CP/MAS 31P(1H) NMR spectrum of complex 1 shows two resonances in a 1:1 intensity ratio, and the CP/MAS 31P(1H) NMR spectrum of complex 3 shows three resonances in a 1:1:1 intensity ratio for reasons that are not yet understood. Though the three phospholes are crystallographically inequivalent (d(AuP) = 2.359 (1), 2.382 (1), and 2.374 (2) angstrom) the molecule has effective Cs symmetry as evidenced by the observation of two 31P resonances in a 2:1 intensity ratio in its CP/MAS 31P(1H) NMR spectrum. Variable-temperature 31P(1H) NMR spectra obtained on solutions of LAuCl + L in various ratios were analyzed to determine the nature of the species present in solution and to gain information regarding their relative stabilities as a function of the nature of the phosphine. 79 refs., 8 figs., 9 tabs

  20. "Faz isso, faz aquilo, mas eu tô caindo...": compreendendo a Doença de Chron "Haz eso, haz aquello, mas yo estoy cayendo...": comprendiendo la enfermedad de Chron "Do this, do that, but I'm falling down...": understanding Chron's disease

    Andrea Castelli

    2007-03-01

    Full Text Available Buscando compreender o significado e o impacto da doença de Chron na vida de seu portador, desenvolvemos este estudo, de natureza qualitativa, optando pela História Oral de Vida como referencial metodológico, tendo como base filosófica a corrente monista, advinda de Hipócrates, em que a prática médica tem por objeto a pessoa doente em sua totalidade, levando em conta seu temperamento e a sua história. A terapêutica deve restabelecer a harmonia da pessoa com seu ambiente e consigo mesma , trata-se de uma medicina integradora e dinâmica, que corresponde aos primórdios da medicina psicossomática. O estudo da doença de Chron, por meio da compreensão psicossomática, permitiu que analisássemos os sintomas, não como corpos estranhos a serem erradicados, mas como mensageiros de um apelo a ser compreendido. Por intermédio deles, pode ser resgatada a história de um indivíduo que, se conscientemente vivida e analisada, permite o desenvolvimento até níveis mais evoluídos de entendimento.Buscando comprender el significado y el impacto de la Enfermedad de Chron en la vida de su portador, desarrollamos este estudio, de naturaleza cualitativa, optando por la Historia Oral de Vida como referencial metodológico, y teniendo como base filosófica la corriente monista, advenida de Hipócrates, donde la práctica médica tiene por objeto a la persona enferma en su totalidad, teniendo en cuenta su temperamento y su historia. La terapéutica debe restablecer la armonía de la persona con su ambiente y consigo misma y se trata de una medicina integradora y dinámica, que corresponde a los inicios de la medicina psicosomática. El Estudio de la enfermedad de Chron, por medio de la comprensión psicosomática, permitió que analizásemos los síntomas, no como cuerpos extraños a ser erradicados, mas como mensajeros de un apelo a ser comprendido. Por intermedio de ellos, puede ser rescatada la historia de un individuo que, si conscientemente vivida

  1. Efficient and facile Ar-Si bond cleavage by montmorillonite KSF: synthetic and mechanistic aspects of solvent-free protodesilylation studied by solution and solid-state MAS NMR.

    Zafrani, Yossi; Gershonov, Eytan; Columbus, Ishay

    2007-08-31

    A facile and efficient method for the cleavage of the Ar-Si bond of various aryl trimethyl silanes is described. When adsorbed on montmorillonite KSF (mont KSF), these arylsilanes readily undergo a solvent-free protodesilylation to the corresponding arenes at room temperature in excellent yields. This approach seems to be superior to the traditional mild methods (i.e., desilylation by TFA, TBAF, CsF), in terms of reaction yield, rate, and environmentally benign conditions. Some mechanistic studies using both solution and solid-state magic-angle spinning (SS MAS) (1)H NMR are also presented. PMID:17676903

  2. Activation of the ACE2/Ang-(1-7)/Mas pathway reduces oxygen-glucose deprivation induced tissue swelling, ROS production, and cell death in mouse brain with angiotensin II overproduction

    Zheng, Jiaolin; Li, Guangze; Chen, Shuzhen; Chen, Ji; Buck, Joshua; Zhu, Yulan; Xia, Huijing; Lazartigues, Eric; Chen, Yanfang; Olson, James E.

    2014-01-01

    We previously demonstrated that mice which overexpress human renin and angiotensinogen (R+A+) show enhanced cerebral damage in both in vivo and in vitro experimental ischemia models. Angiotensin converting enzyme 2 (ACE2) counteracts the effects of angiotensin (Ang-II) by transforming it into Ang-(1-7), thus reducing the ligand for the AT1 receptor and increasing stimulation of the Mas receptor. Triple transgenic mice, SARA, which specifically overexpress ACE2 in neurons of R+A+ mice were use...

  3. Microwave assisted saponification (MAS) followed by on-line liquid chromatography (LC)-gas chromatography (GC) for high-throughput and high-sensitivity determination of mineral oil in different cereal-based foodstuffs.

    Moret, Sabrina; Scolaro, Marianna; Barp, Laura; Purcaro, Giorgia; Conte, Lanfranco S

    2016-04-01

    A high throughput, high-sensitivity procedure, involving simultaneous microwave-assisted extraction (MAS) and unsaponifiable extraction, followed by on-line liquid chromatography (LC)-gas chromatography (GC), has been optimised for rapid and efficient extraction and analytical determination of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) in cereal-based products of different composition. MAS has the advantage of eliminating fat before LC-GC analysis, allowing an increase in the amount of sample extract injected, and hence in sensitivity. The proposed method gave practically quantitative recoveries and good repeatability. Among the different cereal-based products analysed (dry semolina and egg pasta, bread, biscuits, and cakes), egg pasta packed in direct contact with recycled paperboard had on average the highest total MOSH level (15.9 mg kg(-1)), followed by cakes (10.4 mg kg(-1)) and bread (7.5 mg kg(-1)). About 50% of the pasta and bread samples and 20% of the biscuits and cake samples had detectable MOAH amounts. The highest concentrations were found in an egg pasta in direct contact with recycled paperboard (3.6 mg kg(-1)) and in a milk bread (3.6 mg kg(-1)). PMID:26593464

  4. Structure determination of uniformly {sup 13}C, {sup 15}N labeled protein using qualitative distance restraints from MAS solid-state {sup 13}C-NMR observed paramagnetic relaxation enhancement

    Tamaki, Hajime [Hokkaido University, Graduate School of Life Science (Japan); Egawa, Ayako [Osaka University, Institute for Protein Research (Japan); Kido, Kouki [Hokkaido University, Graduate School of Life Science (Japan); Kameda, Tomoshi [National Institute of Advanced Industrial Science and Technology, Biotechnology Research Institute for Drug Discovery (Japan); Kamiya, Masakatsu; Kikukawa, Takashi; Aizawa, Tomoyasu [Hokkaido University, Faculty of Advanced Life Science (Japan); Fujiwara, Toshimichi [Osaka University, Institute for Protein Research (Japan); Demura, Makoto, E-mail: demura@sci.hokudai.ac.jp [Hokkaido University, Faculty of Advanced Life Science (Japan)

    2016-01-15

    Magic angle spinning (MAS) solid-state nuclear magnetic resonance (NMR) is a powerful method for structure determination of insoluble biomolecules. However, structure determination by MAS solid-state NMR remains challenging because it is difficult to obtain a sufficient amount of distance restraints owing to spectral complexity. Collection of distance restraints from paramagnetic relaxation enhancement (PRE) is a promising approach to alleviate this barrier. However, the precision of distance restraints provided by PRE is limited in solid-state NMR because of incomplete averaged interactions and intermolecular PREs. In this report, the backbone structure of the B1 domain of streptococcal protein G (GB1) has been successfully determined by combining the CS-Rosetta protocol and qualitative PRE restraints. The derived structure has a Cα RMSD of 1.49 Å relative to the X-ray structure. It is noteworthy that our protocol can determine the correct structure from only three cysteine-EDTA-Mn{sup 2+} mutants because this number of PRE sites is insufficient when using a conventional structure calculation method based on restrained molecular dynamics and simulated annealing. This study shows that qualitative PRE restraints can be employed effectively for protein structure determination from a limited conformational sampling space using a protein fragment library.

  5. Kadının çalışmasına yönelik tutumlar Azerbaycan – Türkiye karşılaştırması

    QULİYEVA, Shanay

    2014-01-01

     Azerbaycan(AZ) ve Türkiye(TR) Türkleri, uyruk, cinsiyet, cinsiyet rolü,sosyoekonomik düzey değişkenleri dikkate alınarak kadının çalışmasına yöneliktutum ve ev işi yapma sıklığı bakımından karşılaştırılmıştır.Toplam 600 katılımcıdan (300 AZ 300 TR; 300 kadın 300 erkek) veritoplamak amacıyla Kadının Çalışmasına Karşı Tutum Ölçeği, Ev İşi Ölçeği, BemCinsiyet Rolü Envanteri, Sosyoekonomik Düzey Ölçeği ve Kişisel Bilgi Anketikullanılmıştır.Öncelikle Azerbaycan Türklerinden topl...

  6. HONGOS MICORRÍZICOS ARBUSCULARES, Azotobacter chroococcum, Bacillus megatherium Y FitoMas-E: UNA ALTERNATIVA EFICAZ PARA LA REDUCCIÓN DEL CONSUMO DE FERTILIZANTES MINERALES EN Psidium guajava, L. var. Enana Roja cubana

    Leudiyanes Ramos Hernández

    2013-01-01

    Full Text Available El trabajo experimental se desarrolló en la Unidad Básica de Producción Cooperativa (UBPC «Batalla de Jobito» ubicada en el municipio «El Salvador», provincia Guantánamo en el período comprendido entre los años 2007-2009. El objetivo de la investigación fue definir una alternativa de manejo nutricional basada en el empleo de hongos micorrízicos arbusculares (HMA, Azotobacter chroococcum , Bacillus megatherium y el fitoestimulante FitoMas-E, como vía factible para la reducción de la fertilización mineral en el cultivo de la guayaba Enana Roja cubana, sin afectaciones a los rendimientos. Para el montaje del experimento se combinaron 10 g.planta -1 de micorriza (especie: Glomus intraradices ; 2 L.ha -1 de AZOMEG [producto comercial compuesto por Azotobacter chroococcum (2x10 11 ufc x mL -1 y Bacillus megatherium (3.2x10 11 ufc x mL -1 ] y 1 L.ha -1 de FitoMas-E con la reducción gradual de la fertilización mineral de N y P; utilizando el 100, 75, 50 y 25 % respectivamente. Como variable respuesta se midieron la altura de las plantas, los pares de hojas, el número de brindillas, el número de flores y frutos, el diámetro polar y ecuatorial y peso promedio de los frutos; también se calcularon la pérdida de peso postcosecha y el rendimiento. Los resultados experimentales demostraron que la combinación del 75 % de la fertilización mineral con los biofertilizantes y el FitoMas-E fue superior al resto de los tratamientos en estudio para las variables evaluadas, así como posibilitó la reducción del 25 % de la fertilización mineral y obtener una mejor respuesta económica.

  7. Sirds asinsvadu sistēmas bojājumi atkarībā no slimības aktivitātes pakāpes pacientiem ar sistēmisko sarkano vilkēdi

    Grebņeva, Viktorija

    2016-01-01

    KOPSAVILKUMS Sistēmiskā sarkanā vilkēde (SSV) ir hroniska autoimūna saistaudu sistēmas slimība , kas saistīta ar daudzu iekšējo orgānu bojājumiem, ko izraisa autoimūnais process. Manā pētījumā bija atlasīti 52 ambulatorie pacienti. Sieviešu dzimuma izplatība bija lielāka nekā vīriešu dzimuma kontroles grupā, kas nozīmē, ka sievietem ir lielāka nosliece saslimšanai ar sistēmisko sarkano vilkēdi , kā arī palielinātu aterosklerozes risku. Pētījumā izmantoju SLEDAI ( Systemic lupus erythe...

  8. Proton-detected 3D {sup 1}H/{sup 13}C/{sup 1}H correlation experiment for structural analysis in rigid solids under ultrafast-MAS above 60 kHz

    Zhang, Rongchun; Ramamoorthy, Ayyalusamy, E-mail: ramamoor@umich.edu [Biophysics and Department of Chemistry, The University of Michigan, Ann Arbor, Michigan 48109-1055 (United States); Nishiyama, Yusuke [JEOL RESONANCE Inc., Musashino, Akishima, Tokyo 196-8558 (Japan); RIKEN CLST-JEOL Collaboration Center, RIKEN, Yokohama, Kanagawa 230-0045 (Japan)

    2015-10-28

    A proton-detected 3D {sup 1}H/{sup 13}C/{sup 1}H chemical shift correlation experiment is proposed for the assignment of chemical shift resonances, identification of {sup 13}C-{sup 1}H connectivities, and proximities of {sup 13}C-{sup 1}H and {sup 1}H-{sup 1}H nuclei under ultrafast magic-angle-spinning (ultrafast-MAS) conditions. Ultrafast-MAS is used to suppress all anisotropic interactions including {sup 1}H-{sup 1}H dipolar couplings, while the finite-pulse radio frequency driven dipolar recoupling (fp-RFDR) pulse sequence is used to recouple dipolar couplings among protons and the insensitive nuclei enhanced by polarization transfer technique is used to transfer magnetization between heteronuclear spins. The 3D experiment eliminates signals from non-carbon-bonded protons and non-proton-bonded carbons to enhance spectral resolution. The 2D (F1/F3) {sup 1}H/{sup 1}H and 2D {sup 13}C/{sup 1}H (F2/F3) chemical shift correlation spectra extracted from the 3D spectrum enable the identification of {sup 1}H-{sup 1}H proximity and {sup 13}C-{sup 1}H connectivity. In addition, the 2D (F1/F2) {sup 1}H/{sup 13}C chemical shift correlation spectrum, incorporated with proton magnetization exchange via the fp-RFDR recoupling of {sup 1}H-{sup 1}H dipolar couplings, enables the measurement of proximities between {sup 13}C and even the remote non-carbon-bonded protons. The 3D experiment also gives three-spin proximities of {sup 1}H-{sup 1}H-{sup 13}C chains. Experimental results obtained from powder samples of L-alanine and L-histidine ⋅ H{sub 2}O ⋅ HCl demonstrate the efficiency of the 3D experiment.

  9. Eficacia y tolerabilidad de esteroides epidurales vs. Dosis bajas de esteroides mas metamizol mas D-propoxifeno administrados por vía paravertebral en pacientes con síndrome post-laminectomía Efficacy and tolerability of epidural steroids vs low doses of steroids plus metamizol plus propoxyphene administered paravertebrally for postaminectomy syndrome patients

    J. A. Aldrete

    2006-10-01

    de pacientes con radiculopatia post-laminectomia lumbar, al ser tratados con MTP y BPV peridural y un grupo semejante que recibio la mitad de la dosis de MTP, mas BPV, DPN y PPX, en los espacios paravertebral L4-L5 y L5-S1. Los efectos secundarios y la morbilidad predominaron en el grupo tratado con inyecciones peridurales. Inyectando fuera del canal vertebral dosis reducidas de un opiáceo y de un antiinflamatorio, permitió usar solo la mitad de la dosis total de MTP. Ya que se obtiene una mejoría semejante, al evitar los efectos indeseables de los esteroides, así como las posibles complicaciones típicas de inyecciones epidurales, hacen de esta modalidad terapéutica una alternativa definitiva.As the clinical applications of the central antinociceptive action of the non-steroidal antiinflammatory agents have been recognized, their combination with mild doses of opiods has been explored in order to be able to reduce or eliminate the repetitious usage of steroids. A group of 152 adult patients with recurrent radiculopathy after the first laminectomy, at either L4-L5 or L5-S1 levels, in whom periradicular fibrosis and scarring had been shown at MRI exam, was separated at random into two subgroups. Subgroup A included 69 patients that were treated with four epidural injections of 80mg of methylprednisolone (MTP in 3ml of 0.25% bupivacaine (BPV. Changes in pain level, physical activity and the type of medications used were followed using the Pain Progress Score that evaluates five parameters graded from 0 to 2, before initiating treatment and again before each of the three subsequent injections, given at three week intervals. Subgroup B included 73 patients treated by paravertebral injections of 40mg of MTP, 50mg of dipyrone (DPN, 12.5mg of propoxyphene (PPX and 1ml of 0.5% BPV at the L4-L5 and L5-S1 spaces, bilateral. All patients improved clinically without observing statistically significant differences between the two groups; however, there was a significant

  10. 基于智能多代理的能量协调控制在直流微网中的应用%Energy coordination control based on intelligent MAS for DC microgrid

    王晶; 李瑞环; 束洪春

    2013-01-01

    提出一种基于智能多代理技术的能量协调控制方法,用于直流微网的能量管理与电压控制.对直流微网的架构进行设计,实现对光伏电池、燃料电池及蓄电池的数学建模.设计了一个2层的智能多代理系统,对直流微网的并网、解列,以及光伏电池、燃料电池、负荷及蓄电池的能量管理进行协调控制.从微源和负荷各种状态中提取了8个特征量及13种不同动作作为神经网络的输入和输出参数,训练并实现了控制中心Agent的决策器.通过MATLAB/Simulink仿真对光照变化、并网时负荷增加后断网及孤岛时负荷增加后并网这3种算例进行分析,验证了该智能多代理能量协调控制策略的可行性.%An energy coordination control based on intelligent MAS(Multi Agent System) is proposed for the energy management and voltage control of DC microgrid.The framework of DC microgrid is designed and the mathematic models are established for PV,fuel cell and battery.A two-layer MAS is designed for the coordination control:the grid-connection and islanding of DC microgrid and the energy management of PV cells,fuel cells,loads and battery.8 characteristic variables and 13 operations are extracted from various conditions of micro-sources and loads as,respectively,the input and output parameters of a neural network,which is then trained to obtain the decision maker of central Agent.The proposed strategy of energy coordination control is verified by simulation with MATLAB/Simulink for three operating conditions:variable light intensity,islanding after load increase and grid-connection after load increase.

  11. Nevalstiskās organizācijas "Centra Dardedze" sociālo reklāmas kampaņu video materiālu vēstījuma un auditorijas uzmanības piesaistošo elementu analīze

    Kolbina, Inese

    2016-01-01

    Bakalaura darbā „Nevalstiskās organizācijas "Centra Dardedze" sociālo reklāmas kampaņu video materiālu vēstījuma un auditorijas uzmanības piesaistošo elementu analīze” tika analizētas nevalstiskās organizācijas "Centrs Dardedze" seši sociālo reklāmu video materiāli. Analīze balstās uz reklāmas vizuālo vēstījumu un auditorijas uztveres izpēti. Bakalaura darba mērķis ir izpētīt "Centrs Dardedze" reklāmas kampaņas, noskaidrot kā un ar kādiem paņēmieniem konkrētās sociālās reklāmas cenšas mainīt...

  12. Intertīkla protokola balss telefonijas infrastruktūras testu automatizācija nepārtrauktas integrācijas sistēmas ietvaros

    Tkačenko, Valērijs

    2016-01-01

    Kvalifikācijas darbā “Intertīkla protokola balss telefonijas infrastruktūras testu automatizācija nepārtrauktas integrācijas sistēmas ietvaros” tika izstrādāti testēšanas rīki, kas ļauj lietotājam simulēt servisu noturības testus un ērti tos integrēt tādās nepārtrauktās integrācijas platformās kā Jenkins. Testēšanas rīku kopums ir izstrādāts pēc spējās programmatūras izstrādes metodes - Scrum ar JavaScript, HTML5, CSS un Python valodu palīdzību un ir pieejama lietošanas instrukcija angļu val...

  13. Evolution of crystalline aluminates from hybrid gel-derived precursors studied by XRD and multinuclear solid-state MAS NMR; I. Celsian, BaAl{sub 2}Si{sub 2}O{sub 8}

    MacKenzie, K.J.D.; Kemmitt, T. [New Zealand Institute for Industrial Research and Development, P.O. Box 31-310, Lower Hutt (New Zealand)

    1999-01-04

    Hybrid gels of celsian composition were prepared from Al alkoxide, tetrathylorthosilicate (TEOS) and Ba acetate and their structure evolution was studied up to 1300C by thermal analysis and X-ray diffraction. Information on their pre-crystallization behaviour was also provided by {sup 27}Al, {sup 29}Si and {sup 137}Ba MAS NMR spectroscopy. Apart from some excess Ba acetate which decomposed to traces of BaCO{sub 3} and BaO by ca. 500C, the gels are X-ray amorphous and relatively homogeneous, and begin to crystallize to hexagonal celsian at 900C. From {approx}500C onwards, an Al-substituted tetrahedral SiO{sub 4} framework begins to be established, evidenced by a progressive increase in the tetrahedral {sup 27}Al sites and the Q{sup 4}(4Al) {sup 29}Si resonance. Migration of Ba into the polyhedral celsian sites occurs much more slowly. A small amount of mullite and Ba{sub 2}SiO{sub 4} which crystallize from Al-rich and Ba-rich regions, respectively, also form crystalline celsian in secondary reactions at ca. 1100C. The observation of a {sup 27}Al shoulder at ca. 36 ppm at 500-900C may arise from Ba-poor mullite-like regions. (Copyright (c) 1999 Elsevier Science B.V., Amsterdam. All rights reserved.)

  14. Evolution of crystalline aluminates from hybrid gel-derived precursors studied by XRD and multinuclear solid-state MAS NMR; I. Celsian, BaAl2Si2O8

    Hybrid gels of celsian composition were prepared from Al alkoxide, tetrathylorthosilicate (TEOS) and Ba acetate and their structure evolution was studied up to 1300C by thermal analysis and X-ray diffraction. Information on their pre-crystallization behaviour was also provided by 27Al, 29Si and 137Ba MAS NMR spectroscopy. Apart from some excess Ba acetate which decomposed to traces of BaCO3 and BaO by ca. 500C, the gels are X-ray amorphous and relatively homogeneous, and begin to crystallize to hexagonal celsian at 900C. From ∼500C onwards, an Al-substituted tetrahedral SiO4 framework begins to be established, evidenced by a progressive increase in the tetrahedral 27Al sites and the Q4(4Al) 29Si resonance. Migration of Ba into the polyhedral celsian sites occurs much more slowly. A small amount of mullite and Ba2SiO4 which crystallize from Al-rich and Ba-rich regions, respectively, also form crystalline celsian in secondary reactions at ca. 1100C. The observation of a 27Al shoulder at ca. 36 ppm at 500-900C may arise from Ba-poor mullite-like regions. (Copyright (c) 1999 Elsevier Science B.V., Amsterdam. All rights reserved.)

  15. The Advances and Perspective Utilization of Meiosis Activating Sterols in Assisted Reproduction%促减数分裂甾醇(MAS)在附助生殖生物学中的作用及应用前景

    夏国良; 吕忠显

    2000-01-01

    促减数分裂甾醇(Meiosis-activating Sterols, MAS)是近年来新发现的能够促进哺乳动物生殖细胞减数分裂恢复的物质.其中,来自人卵泡液的FF-MAS和来自牛睾丸组织T-MAS能诱导体外培养的小鼠卵母细胞减数分裂的恢复,可能在促性腺激素调控配子的成熟过程中扮演重要的生理性角色.本文结合我们的研究结果,就MAS在性腺的合成及分布、与卵母细胞减数分裂恢复及精子形成的关系以及MAS在附助生殖生物学中可能扮演的角色综述如下.

  16. Cirrus cloud optical and microphysical property retrievals from eMAS during SEAC4RS using bi-spectral reflectance measurements within the 1.88 µm water vapor absorption band

    Meyer, Kerry; Platnick, Steven; Arnold, G. Thomas; Holz, Robert E.; Veglio, Paolo; Yorks, John; Wang, Chenxi

    2016-04-01

    Previous bi-spectral imager retrievals of cloud optical thickness (COT) and effective particle radius (CER) based on the Nakajima and King (1990) approach, such as those of the operational MODIS cloud optical property retrieval product (MOD06), have typically paired a non-absorbing visible or near-infrared wavelength, sensitive to COT, with an absorbing shortwave or mid-wave infrared wavelength sensitive to CER. However, in practice it is only necessary to select two spectral channels that exhibit a strong contrast in cloud particle absorption. Here it is shown, using eMAS observations obtained during NASA's SEAC4RS field campaign, that selecting two absorbing wavelength channels within the broader 1.88 µm water vapor absorption band, namely the 1.83 and 1.93 µm channels that have sufficient differences in ice crystal single scattering albedo, can yield COT and CER retrievals for thin to moderately thick single-layer cirrus that are reasonably consistent with other solar and IR imager-based and lidar-based retrievals. A distinct advantage of this channel selection for cirrus cloud retrievals is that the below-cloud water vapor absorption minimizes the surface contribution to measured cloudy top-of-atmosphere reflectance, in particular compared to the solar window channels used in heritage retrievals such as MOD06. This reduces retrieval uncertainty resulting from errors in the surface reflectance assumption and reduces the frequency of retrieval failures for thin cirrus clouds.

  17. Comparison of the thermal gain of the more commonly roofing systems used in Mexico`s social dwellings; Comparacion de la ganancia termica de los sistemas de techumbre mas comunmente utilizados en la vivienda social en Mexico

    Fernandez, Alfredo [Division de Estudios de Posgrado de la Facultad de Arquitectura UNAM, Mexico, D. F. (Mexico); Morillon, David [Comision Nacional para el Ahorro de Energia (CONAE), Mexico, D. F. (Mexico)

    1996-12-31

    Under the frame of discussion of the Mexican Official Standard 008 (Integral Energy Efficiency in non-residential Buildings), and with the purpose of assessing its feasibility and possible extension to residential buildings, this paper, whose objective is to evaluate the roofing systems (opaque) more commonly utilized on the social dwellings in Mexico, is presented. To perform this evaluation the heat gains by conduction through the roofing in different cities were obtained. Later on these heat gains were compared against the energy consumption that would produce its removal in each one of the cases. Finally, thermal resistance values (R) that allow, in significant form diminishing such gains, are presented, producing in consequence, a thermally efficient building. [Espanol] Dentro del marco de discusion de la Norma Oficial Mexicana 008 (Eficiencia energetica integral en edificios no residenciales), y con el fin de evaluar su factibilidad y posible extension hacia las edificaciones residenciales, se presenta este trabajo que tiene como objetivo la evaluacion de los sistemas de techumbre (opacos) mas comunmente utilizados dentro de la vivienda social en Mexico. Para realizar esta evaluacion se obtuvieron las ganancias por conduccion a traves del techo para distintas ciudades. Posteriormente, se compararon dichas ganancias contra el consumo de energia que produciria la remocion de las mismas en cada uno de los casos. Finalmente, se proponen valores de Resistencia Termica (R) que permitan disminuir de manera importante dichas ganancias teniendo, en consecuencia, una edificacion energeticamente eficiente.

  18. Examination of the structure in solid state of amino analogs of 4,4‧-[1,5-pentanediylbis(oxy)]bisbenzonitrile by means of X-ray diffraction, 13C CP/MAS NMR, and theoretical calculations

    Maciejewska, Dorota; Wolska, Irena; Żabiński, Jerzy

    2008-05-01

    A single crystal of X-ray diffraction structures is presented for 4,4'-[1,5-(3-oxapentanediylbis(amino))]bisbenzonitrile 2 and 4,4'-[1,5-( N-methyl-3-azapentane-diylbis(oxy))]bisbenzonitrile 3. The molecular structures of these derivatives differ especially in conformations of the central linker: in 2 this linker adopts a trans/ gauche conformation, whereas in 3 - a fully extended conformation. The N atoms in various positions of the aliphatic linker change dramatically the molecular packing mode of both bisnitriles. But in both cases the nitrile groups take part in intermolecular hydrogen bonds: a type of N sbnd H···N in 2 and of C sbnd H···N in 3. Various conformations of both molecules were reflected in 13C CP/MAS NMR spectra in solid state as single and double resonance patterns for 2 and 3, respectively. A preliminary anticancer assay against 60 cell lines of 3 reveals strong growth inhibition of leukemia, melanoma, and renal cancer cells.

  19. Sarmiento: su perfil masónico

    Arrondo, César Aníbal

    2001-01-01

    El presente trabajo tiene por objeto sacar a la luz un aspecto por demás interesante de la personalidad de Domingo Faustino Sarmiento, como lo fue su pertenencia a la Francmasonería. En tal sentido me resulta de interés realizar una breve reflexión sobre los principios y postulados que dieron origen a la existencia de esta “Institución filosófica” desde los tiempos.

  20. Using THOMAS for Service Oriented Open MAS

    Julian, V.; Rebollo, M.; Argente, E.; Botti, V.; Carrascosa, C.; Giret, A.

    Recent technological advances in open systems have imposed new needs on multi-agent systems. Nowadays, open systems require open autonomous scenarios in which heterogeneous entities (agents or services) interact to fulfill the system goals. This impose the need for open architectures and computational models for large-scale open multi-agent systems based on service-oriented approaches. THOMAS is a new architecture specifically addressed for the design of virtual organizations for open systems. In this paper we present a case study that exemplifies the usage of THOMAS for implementing a management system of a travel agency.

  1. EAD sim, mas com qual biblioteca?

    Solange Puntel Mostafa

    2003-07-01

    Full Text Available Discute a importância da biblioteca virtual para o ensino a distância de qualquer curso; para tal o bibliotecário deve ser elemento indispensável na equipe de EAD uma vez que ele conhece as fontes e as formas de organização da informação, o que lhe possibilita atuar inclusive como tutor. Recomenda-se a intervenção do bibliotecário já na fase de planejamento do curso de tal forma que a biblioteca resultante seja um produto/processo tão importante quanto os demais componentes curriculares e customizada ao curso em questão. Destaca a gestão da informação nos sites educacionais, essa outra modalidade de EAD na formação continuada de professores.

  2. Are M&As Suffocating Chinese Business?

    2007-01-01

    A series of recent foreign capital mergers and acquisitions (M&A) of Chinese businesses has raised concern about China’s economic security. Will these actions damage China’s national interests and is it necessary for China to set a bottom line in this fi

  3. Zastosowanie cieploprzewodzqcych mas klejowych w odlewnictwie

    Z. Mirski

    2008-03-01

    Full Text Available Klcjc oparte na Swicy rnetaksylowej zaliczane sq do materirhbw nicprzewodqcych. Przcz nnpclnicnic klcju 2-sklndnikowcgo AgonictF330 obcq f~i7~wq postaci proszko~vychn apclniaczy mctaIo~vychm otna uzyskd wtnSciwoSci cic~lopr;/cwod~~mcacs klcjo~ycli.M ntcriatyte mogq by6 rvy koszystanc do rcgencracji powicrzchnio~vych wad odiewniczych do naprawy takich uszkodzcn jak pckni~cia, odpryskii wykruszcnia w odlewan ych clcmentach maszyn. Cieptoprtewodqcc masy kIcjorvc sq wykorzys~ywancd o hudowy prototypow~ychform odlcwniczych np, do poliuretanbw, stop6w niskotopliwych i w tcchnoIogiach rapid prototyping. Pominry przcwodnofci cicplncj lnnsklejqcych wykonano przy utyciu aparatu Pocnsgena, w tcmpcratunch: 30. 100 i 150 'C. Wstqpnc pon~iary wsp6lczynnikn przcicodnokicicplncj dla utwardzonych ktcjow wykazaly wartoit 1 = 0,2 W1m.K.W celu uzyskmin wlakiwoSci cicplopnc~vod~~cymcahs klcjoych (12 0.6W1rn.K I] tcprotmdmno do nich naptnincze w postaci proszk6w Vc i AI. Zachowujqc jcszczc wtaSci~voCcik lcjqcc micsmniny kl ju z ~iapclniacczamimetaloyni[ udalo sic wprowadzit 58% obi, proszk6w. Dzicki tcmu dla masy klcjowcj z nnpclniaczcn~n Iulninioivym udalo sic uqsknf itarioSCvs$lcqnnika pi-zcvdnoSci cicplncj 1, = 1,15 W/tn.K, w tempmturrr: otoczcnia 30 "C.

  4. Violencia contra las mujeres y alguien mas...

    Piatti, Mª de Lujan

    2013-01-01

    La violencia contra las mujeres es una clara vulneración a sus derechos humanos. Es una violencia ejercida contra ellas por el solo hecho de ser mujeres; no se trata de casos aislados, sino que constituye un fenómeno social, presente en la sociedad actual sin distinción de razas, lugar geográfico, nivel cultural, religión, sistema político o económico. Investigar sobre “La violencia contra las mujeres y alguien más…”, desde una mirada multidisciplinar, significa bucear en las profundidades de...

  5. (29)Si, (47)Ti, (49)Ti and (195)Pt solid state MAS NMR spectroscopic investigations of ternary silicides TPtSi, germanides TPtGe (T = Ti, Zr, Hf) and stannide TiPtSn.

    Benndorf, Christopher; Eckert, Hellmut; Pöttgen, Rainer

    2016-05-10

    Eight ternary tetrelides TPtX (T = Ti, Zr, Hf; X = Si, Ge, Sn) were synthesized from the elements by arc-melting and subsequent annealing. TiPtSi, ZrPtSi, ZrPtGe, HfPtSi and HfPtGe crystallize with the orthorhombic TiNiSi type structure, in the space group Pnma. The structures of HfPtSi (a = 654.44(9), b = 387.97(6), c = 750.0(1) pm, wR2 = 0.0592, 411 F(2) values, 20 variables) and HfPtGe (a = 660.36(7), b = 395.18(4), c = 763.05(8) pm, wR2 = 0.0495, 430 F(2) values, 20 variables) were refined from single crystal X-ray diffractometer data. TiPtSn adopts the cubic MgAgAs type. TiPtGe is dimorphic with a TiNiSi type high-temperature modification which transforms to cubic LT-TiPtGe (MgAgAs type). All phases were investigated by high resolution (29)Si, (47)Ti, (49)Ti and (195)Pt solid state MAS NMR spectroscopy. In the cubic compounds, the (47/49)Ti NMR signals are easily detected owing to the absence of quadrupolar broadening effects. The (195)Pt resonances of the orthorhombic compounds are characterized by strongly negative isotropic Knight shifts and large Knight shift anisotropies, whereas positive isotropic Knight shifts and no anisotropies are observed for the cubic compounds. These results indicate that the phase transition in TiPtGe is associated with dramatic changes in the electronic properties. Within each group of isotypic compounds the isotropic (29)Si, (47/49)Ti and (195)Pt Knight shifts show systematic dependences on the transition metal or tetrel atomic number, suggesting that the numerical values are influenced by the electronegativities of the metallic (or metalloid) neighbours. PMID:27097719

  6. Experimental (X-ray, (13)C CP/MAS NMR, IR, RS, INS, THz) and Solid-State DFT Study on (1:1) Co-Crystal of Bromanilic Acid and 2,6-Dimethylpyrazine.

    Łuczyńska, Katarzyna; Drużbicki, Kacper; Lyczko, Krzysztof; Dobrowolski, Jan Cz

    2015-06-01

    A combined structural, vibrational spectroscopy, and solid-state DFT study of the hydrogen-bonded complex of bromanilic acid with 2,6-dimethylpyrazine is reported. The crystallographic structure was determined by means of low-temperature single-crystal X-ray diffraction, which reveals the molecular units in their native protonation states, forming one-dimensional infinite nets of moderate-strength O···H-N hydrogen bonds. The nature of the crystallographic forces, stabilizing the studied structure, has been drawn by employing the noncovalent interactions analysis. It was found that, in addition to the hydrogen bonding, the intermolecular forces are dominated by stacking interactions and C-H···O contacts. The thermal and calorimetric analysis was employed to probe stability of the crystal phase. The structural analysis was further supported by a computationally assisted (13)C CP/MAS NMR study, providing a complete assignment of the recorded resonances. The vibrational dynamics was explored by combining the optical (IR, Raman, TDs-THz) and inelastic neutron scattering (INS) spectroscopy techniques with the state-of-the-art solid-state density functional theory (DFT) computations. Despite the quasi-harmonic approximation assumed throughout the study, an excellent agreement between the theoretical and experimental data was achieved over the entire spectral range, allowing for a deep and possibly thorough understanding of the vibrational characteristics of the system. Particularly, the significant influence of the long-range dipole coupling on the IR spectrum has been revealed. On the basis of a wealth of information gathered, the recent implementation of a dispersion-corrected linear-response scheme has been extensively examined. PMID:25961154

  7. Analysis of mercerization process based on the intensity change of deconvoluted resonances of {sup 13}C CP/MAS NMR: Cellulose mercerized under cooling and non-cooling conditions

    Miura, Kento [Mitsubishi Rayon Co., Ltd. Otake Research Laboratories (Japan); Nakano, Takato, E-mail: tnakano@kais.kyoto-u.ac.jp [Laboratory of Biomaterials Design, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University (Japan)

    2015-08-01

    The area intensity change of C1, C4, and C6 in spectrum obtained by {sup 13}C CP/MAS NMR and the mutual relationship between their changes were examined for cellulose samples treated with various concentrations of aqueous NaOH solutions under non-cooling and cooling conditions. The area intensity of C1-up and C6-down changed cooperatively with that of C4-down which corresponds to the crystallinity of samples: “-up” and “-down” are the up- and down- field component in a splitting peak of NMR spectrum, respectively. The intensity change of C1-up starts to decrease with decreasing in that of C4-down after that of C6-down is almost complete. These changes were more clearly observed for samples treated under cooling condition. It can be suggested that their characteristic change relates closely to the change in conformation of cellulose chains by induced decrystallization and the subsequent crystallization of cellulose II, and presumed that their changes at microscopic level relate to the macroscopic morphological changes such as contraction along the length of cellulose chains and recovery along the length. - Highlight: • Samples were mercerized at various NaOH concentrations under non-cooling and cooling. • The intensity change of C1 starts immediately after that of C6 is complete. • The creation of cell-II starts when decrystallization proceeds to a certain state. • This change relates closely to the change in conformation of cellulose chains. • The above change is more clearly found for samples treated under cooling.

  8. Fe/ZSM-5 prepared by sublimination of FeCl{sub 3}: The structure of the Fe species as determined by IR, {sup 27}Al MAS NMR, and EXAFS spectroscopy

    Marturano, P.; Drozdova, L.; Kogelbauer, A.; Prins, R.

    2000-05-15

    The state of the iron in two different Fe/ZSM-5 samples prepared by sublimation of FeCl{sub 3} was investigated by EXAFS, IR, {sup 27}Al MAS NMR, XRD, and nitrogen adsorption measurements. In one Fe/ZSM-54 (Fe/Al = 1) sample, EXAFS revealed for the first time the presence of diferric (hydr)oxo-bridged binuclear clusters, whose structures differ from those postulated in the literature, resembling that of the methane monooxygenase enzyme. IR showed that binuclear Fe complexes are located at the ion-exchange positions of the zeolite, compensating one or two lattice charges. The remainder of the charge-compensating one or two lattice charges. The remainder of the charge-compensating sites are Broensted hydroxyls. On both zeolites, the NMR detection of the framework Al atoms (54 ppm) is strongly perturbed by the paramagnetic effects induced by the Fe ions. The intensity of this peak parallels that of the Broensted hydroxyls in the IR spectra, thus reflecting the presence of Fe species at ion-exchange positions. In a second Fe/ZSM-5 (Fe/Al = 0.8) sample, the iron was present predominantly in the form of large hematite particles (EXAFS, XRD), although a minor fraction of binuclear species might be present as well. The formation of different species seems to be related to different hydrolysis processes occurring on the two zeolites upon washing of the preparation after the sublimation of FeCl{sub 3}. It is also suggested that the final state of the Fe depends on the presence of extraframework Al species as well as the crystallite size of the zeolite used.

  9. Madde-Ruh Çatışmasından Simeranya’ya Kaçış: Yalnızız Escape to Simeranya from Item-Spirit Conflict : Yalnızız

    Adem POLAT

    2012-09-01

    Full Text Available This article is a consideration of matter-soul conflict which has a close relationship with the thought dimension established upon historical, sociological and cultural ground in the novel of Yalnızız of Peyami Safa, who has a crucial place in the Turkish Novel in the Republic period. The conflict in question and the paradoxes ingrained in society, emerges besides moral corruption. More importantly, Yalnızız is a novel which has the motivation to produce remedial solutions within aplan like Simeranya. In this respect, an utopian fiction, Simeranyaphenomenon’s effect on the character Samim, displays the intellectualground upon which matter-soul conflict built. These grounds do notonly include the ideal of the world the artist yearns for, but also theydisclose the factual values that will be conveyed as a message throughthe confrontation of matter-soul in the center of the novel. Inconsequence, even though it is beyond reality like Simeranya, inYalnızız novel, the crucial parameters for Turkish thought areconsidered on the agenda by Peyami Safa, as a solution suggestion tothe enduringly growing phenomena and which are carried towardstoday since our Westernization history.In other words, Simeranya is a sheltering territory from theanxiety that are felt due the social and actual realm. If it must be told inthe words of the KORA thematic system, Simeranya, is the place whichgathers dialectical affirmation of the conflicting values in thephenomenal realm of the ideal values. This work aims to scrutinize theintellectual grounds that are describable through the theme of mattersoulconfrontation in question. Bu makale Cumhuriyet devri Türk romanında önemli bir yere sahip olan Peyami Safa’nın Yalnızız romanında yer alan tarihsel, sosyolojik ve kültürel zemine bağlı olarak ortaya çıkmış olan düşünce boyutuyla yakından ilişki kuran madde-ruh çatışmasının roman üzerindeki bir değerlendirmesidir. Söz konusu

  10. Adiposidade corporal, mas não resistência insulínica, associa-se ao polimorfismo -675 4G/5G no gene PAI-1 em uma amostra de crianças mexicanas

    Ulises de la Cruz-Mosso

    2013-10-01

    Full Text Available OBJETIVO: Elaboramos este estudo para avaliar se o polimorfismo -675 4G/5G no gene inibidor 1 do ativador do plasminogênio se associa à obesidade e à resistência insulínica em crianças mexicanas. MÉTODOS: Foi realizado um estudo transversal em 174 crianças, 89 delas com peso normal e 85 obesas, variando sua idade de 6 a 13 anos. Todas as crianças eram do estado de Guerrero e foram recrutadas de três escolas primárias na cidade de Chilpancingo, México. Os níveis de insulina foram determinados por prova imunoenzimática. Foi usado o modelo de avaliação da homeostase para determinar resistência insulínica. O polimorfismo -675 4G/5G no gene PAI-1 foi analisado pelo método reação de polimerase em cadeia-polimorfismo no comprimento dos fragmentos de restrição. RESULTADOS: A prevalência de resistência insulínica no grupo obeso foi mais alta (49,41% do que no grupo com peso normal (16,85%. O polimorfismo 4G/5G do PAI-1 foi encontrado em equilíbrio de Hardy Weinberg. O genótipo 4G/5G contribuiu para um aumento significativo da relação cintura-quadril (β = 0,02, p = 0,006, da circunferência da cintura (β = 4,42, p = 0,009 e da espessura da prega subescapular (β = 1,79, p = 0,04, mas não se relacionou com a resistência insulínica. CONCLUSÃO: O genótipo -675 4G/5G do gene PAI-1 se associou a aumento da adiposidade corporal em crianças mexicanas.

  11. Reading motivation and engagement scale: Turkish adaptation studyOkuma motivasyonu ve okumaya adanmışlık ölçeği: Türkçeye uyarlama çalışması

    Mustafa Yıldız

    2015-11-01

    Full Text Available The purpose of this study was to examine the adaptation, reliability and validity of       “the reading motivation and reading engagement scale” that was developed by Guthrie and Klauda (2014 to determine the reading motivation and reading engagement levels of students. The study was conducted with 376 students attending to 5th,, 6th, 7th and 8th grades in the province of Ankara. The scale includes 39 items and 7 factors namely intrinsic motivation, perceived competence, perceived difficulty, value, devalue dedication and avoidance. SPSS 17.00 and AMOS 18 softwares were used for data analysis.The factor structure of the scale was faithful to the original. The reliability coefficient of the scale was found to be .84 using the internal consistency method.According to the results of the analysis, the psychometric properties of the scale were suitable to use, valid, and reliable.According to the results of the analysis, the psychometric properties of the scale were suitable to use, valid, and reliable.   Özet Bu çalışmanın amacı, öğrencilerin okuma motivasyonlarını ve okumaya adanmışlık düzeylerini belirlemek amacıyla Guthrie ve Klauda (2014 tarafından geliştirilmiş olan “Okuma Motivasyonu ve Okumaya Adanmışlık Ölçeği”nin uyarlama, geçerlik ve güvenirlik çalışmasını yapmaktır. Araştırmanın çalışma grubunu Ankara ilinde eğitim görmekte olan ve 5,6,7 ve 8. sınıfa devam eden 376 öğrenci oluşturmaktadır.Ölçek 39 madde ve içsel motivasyon, yeterlilik, güçlük, değer verme, değer vermeme, bağlılık ve kaçınma olarak adlandırılan 7 faktörden oluşmaktadır. Verilerin analizi SPSS 17.00 ve AMOS 18 programlarıyla yapılmıştır.Ölçek orjinaline uygun faktör yapısında oluşturulmuştur. Ölçeğin güvenirlik katsayısı iç tutarlılık yöntemiyle .84  olarak hesaplanmıştır. Yapılan analizlerin sonuçlarına göre, ölçeğin psikometrik özelliklerinin kullan

  12. Reading attitude scale: The reliability and validity studyOkuma tutum ölçeği: Geçerlik ve güvenirlik çalışması

    Orhan Çakıroğlu

    2015-04-01

    Full Text Available The purpose of this study was to examine the adaptation, reliability and validity of a “reading attitude scale” that was developed by McKenna and Kear (1990 to determine the reading attitudes of students.  The study was conducted with 318 students attending to 5th,6th,7th, and 8th grades in Trabzon. The scale includes 20 items and two factors called as leisure time reading and academic reading. The analyses were conducted in SPSS 20.0 and AMOS v.22. The final Turkish version of the scale included 20 items and two factors as it was in the original scale. The internal consistency reliability of the scale was 0.84 and 0.78 according to test-retest analysis. This is the first study that examines the reliability and validity of the Turkish version of the scale by using advanced statistics to determine the psychometric characteristics of the measure. Based on the study findings, the Reading Attitude Survey is a practical, feasible, and an understandable measurement tool.ÖzetBu çalışmanın amacı, öğrencilerin okumaya yönelik tutumlarını belirlemek amacıyla Mckenna ve Kear (1990 tarafından geliştirilmiş olan “Okumaya Yönelik Tutum Ölçeği”nin (OTÖ uyarlama, geçerlik ve güvenirlik çalışmasını yapmaktır. Araştırmanın çalışma grubunu Trabzon ilinde eğitim görmekte olan ve 5.6.,7., ve 8. sınıfa devam eden toplam 318 öğrenci oluşturmuştur. Veriler SPSS 20.0 ve AMOS versiyon 22 paket programlarıyla yapılmıştır. Ölçek orijinaline uygun madde sayısında ve faktör yapısında oluşmuştur. DFA uyum indeksi ve madde faktör dağılımları istatistiksel olarak beklentilere uygun açığa çıkmıştır.   Ölçeğin güvenirlik katsayısı iç tutarlılık yöntemi ile 0.84 test-tekrar test yöntemiyle 0.78 olarak hesaplanmıştır. İlk kez bu kapsamda ileri veri analizleri ile incelemesi gerçekleştirilen aracın psikometrik özelliklerinin kullanılmaya elverişli geçerli ve güvenilir oldu

  13. ПРОБЛЕМЫ «СЕЛЕКЦИИ С ПОМОЩЬЮ МАРКЕРОВ» (MAS)

    Глазко, В.

    2013-01-01

    Рассматриваются собственные и литературные данные о результатах использования современных методов«селекции с помощью маркеров» (MAS) в животноводстве. Обсуждаются направления увеличения их эффективности, в частности, путем выявления геномных мишеней естественного отбора, усложняющих выявление геномных элементов, полиморфизм которых прямо ассоциирован с изменчивостью хозяйственно-ценных признаков. В этих целях предлагается использование для геномного сканирования полилокусное генотипирование н...

  14. Breeding of Trans-Bt Gene Rice Varieties with Pest Resistance by Means of Marker-Assisted Selection (MAS)%利用分子标记辅助选择培育转 Bt基因抗虫水稻

    张琳; 魏志刚; 裴婷婷; 胡颂平; 余霞; 张静; 罗利军; 叶水峰; 刘国兰

    2014-01-01

    In order to breed trans-Bt gene rice materials with pest resistance,three Bt genes,cry1Ac/Ab, cry1C* and cry2A*,from the donor parents developed by Huazhong Agricultural University were introgressed into Xiushui123,Xiangqing,Hanhui No.3 and Huhan1B,namely Minghui63( Cry1C*) ,Minghui63( Cry2A*) and Minghui63 ( Cry1Ab/Ac) respectively using the method of traditional hybrid;then the new type lines of trans-Bt gene rice were by obtained means of maker-assisted selection ( MAS) ,screening of basta test in the field and test paper strip detection.In addition trans-gene rice lines of good pest resistance and fine agricultural traits were scteened by using the assays of insect-resistance in the field under normal field management but without spraying pesticide in the whole bearing stage of these trans-gene lines providing the materials for fur-ther anti-pest breeding.The homozygous lines with cry1C* and cry2A were obtained in the different back-grounds ( Xiushui123,Xiangqing and Huhan1B) respectively by the detection of PCR and test paper strip.At the same time,Bt lines showed excellent resistance to rice leaf roller in the field under the condition of its natu-ral occurrence.%为培育转Bt基因抗虫水稻品种材料,以传统杂交的方法将3个Bt明恢63中的Bt基因cry1C*、cry2A*和cry1Ac/Ab,转育到水稻品种秀水123、湘晴、旱恢3号和沪旱1B中;以回交二代自交三代(BC2F3)水稻叶片为材料,利用分子标记辅助选择、田间除草剂筛选和试纸条检测方法,以获得新型转Bt基因株系。并在全生育期实行除不喷施农药外的正常田间管理的转基因植株的田间抗虫鉴定方法筛选出抗虫性良好、农艺性状优良的株系,为后续抗虫育种提供材料。 BC2 F3代PCR检测及试纸条检测的结果显示分别获得cry1C*和cry2A*基因的沪旱1B、秀水123和湘晴的纯合株系。同时,在田间自然诱发虫害条件下,转基因株系对稻纵卷叶螟虫表现出强抗性。

  15. An X-ray diffraction and P-31 MAS NMR study of rare-earth phosphate glasses, (R2O3)(x)(P2O5)(1-x), x=0.175-0.263, R = La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er

    Cole, Jacqueline M.; van Eck, Ernst R. H.; Mountjoy, Gavin; Anderson, Ruth; Brennan, Tessa; Bushnell-Wye, Graham; Newport, Robert J; Saunders, George A.

    2001-01-01

    An X-ray diffraction and P-31 MAS NMR study of rare-earth phosphate glasses of composition, (R2O3)(x)P2O5)(1-x), where x = 0.175-0.263 and R = La-Er (except for Pm), is presented. The structures of these materials were investigated as a function of (a) rare-earth atomic number and (b) glass composition, The results show an increase in rare-earth coordination number from six to seven as the rare-earth ion increases in size. This effect is most evident for the rare earths, Ce, Pr and Nd, and ap...

  16. School counselors’ roles scale: A surveyPsikolojik danışman rolleri ölçeği: Bir tarama çalışması

    Ömer Faruk Kabakçı

    2013-06-01

    şarı, rehber öğretmeni tanıma ve rehberlik servisine gitme değişkenlerine göre farklılaşıp farklılaşmadığının belirlenmesi amaçlanmıştır. Araştırma katılımcılarını 190’ı kız, 192’si erkek olmak üzere devlet lisesine devam eden toplam 382 lise öğrencisi oluşturmuştur. Ayrıca araştırmaya 69’u kız, 61’i erkek olmak üzere 130 lise öğrencisi de testin tekrarı çalışması için dâhil edilmiştir. Öğrencilerin psikolojik danışman rolleri algılarını belirlemek için araştırmacıların geliştirdiği Psikolojik Danışman Rolleri Ölçeği kullanılmış, bağımsız değişkenlerle ilgili veri toplamak için yine araştırmacıların geliştirdiği kişisel bilgi formundan yararlanılmıştır. Verilerin analizinde açımlayıcı faktör analizi, iç tutarlığı belirmek için Cronbach alfa ve test-tekrar test güvenirliği için Pearson Korelasyon katsayısı ile değişkenler arası fark için ANOVA ve t testi kullanılmıştır. Deneme formu 32 maddelik olan ölçeğin, açımlayıcı faktör analizi sonucunda üç faktörlü (akademik, kariyer kişisel/sosyal18 maddelik bir yapı gösterdiği bulunmuştur. Yapılan analizler ile ölçeğin yeterli geçerlik ve güvenirlik düzeyine sahip olduğu belirlenmiştir. Bulgulara göre öğrencilerin psikolojik danışman rollerine yönelik algıları cinsiyet, sınıf, başarı düzeyi, rehber öğretmeni tanıma ve rehberlik servisine gitme değişkenlerine göre farklılaşmaktadır. Bulgular alan yazın ışığında tartışılmış ve bulgulara dayalı olarak bazı önerilere yer verilmiştir.

  17. Free time management scale: Validity and reliability analysisBoş zaman yönetimi ölçeği: Geçerlik- güvenirlik çalışması

    Beyza Merve Akgül

    2015-12-01

    Full Text Available The aim of this research is to adapt the Free time management scale (Wei-Ching Wang,  Chin-Hsung Kao, Tzung-Cheng Huan, Chung-Chi Wu, 2011 to Turkish and to examine its psychometric properties. The research was conducted on 447 university students from various faculties of Gazi University, Turkey. First of all, lingual equivalence of the scale was applied.  Explanatory (EFA and confirmatory factor analysis (CFA were used for validity analysis of the scale. In the result of the EFA, Being in English originally, this scale consisted of 15 items and 4 dimensions. While the number of the items were exactly the same in the ‘leisure attitude’ and ‘scheduling’, ‘goal setting and technique’ was 6 items and ‘evaluating’ was 3 items. Confirmatory factor analysis (CFA was applied to test the four factor construct of the scale. Internal consistency coefficient of the whole scale was found to be .83 and test retest reliability of the scale was found to be .86. To this end, According to the validity and reliability analyses, it was found that this scale is a reliable and valid scale to be used to determine free time management  of university students in Turkey.   Özet Bu çalışmanın amacı, Wei-Ching Wang,  Chin-Hsung Kao, Tzung-Cheng Huan, Chung-Chi Wu isimli kişiler (2011 tarafından geliştirilen Boş Zaman Yönetimi Ölçeğini Türkçeye uyarlamak ve ölçeğin geçerlik ve güvenirlik analizlerini yapmaktır. Araştırmaya Gazi Üniversitesi’nin çeşitli bölümlerinde öğrenim gören 447 üniversite öğrencisi katılmıştır. Öncelikle ölçeğin dilsel eşdeğerliği incelenmiş ve dilsel eşdeğerliğe sahip olduğu görüldükten sonra geçerlik ve güvenirlik analizleri yapılmıştır. Ölçeğin geçerlik çalışmasına, açımlayıcı (AFA ve doğrulayıcı faktör analizleri (DFA kullanılmıştır. AFA sonucunda, , 15 madde ve 4 alt boyuttan oluşan bir ölçme aracı elde edilmiş ve alt boyutlarda yer alan

  18. Spectrophotometric determination of tracea mounts of lanthanum in zinc alloy with new chromogenic reagent 5-MasA-DBF%新显色剂二溴氟基偶氮甲胂分光光度法测定热镀锌中痕量镧

    倪英萍; 史俊霞; 凌程凤; 宗俊

    2006-01-01

    报道了新显色剂3-[(2-胂羧基-5-甲基苯)偶氮]-6-[(2,6-二溴-4-氟苯)偶氮]-4,5-二羟基-2,7-萘二磺酸(简称5-MasA-DBF)测定锌合金中痕量镧的分光光度法.在0.1 mol/L盐酸介质中,镧与新显色剂5-MasA-DBF形成了配合比为1∶2蓝紫色配合物,其最大吸收波长为640 nm,表观摩尔吸光系数ε=1.30×106 L·mol-1·cm-1,线性范围为0~24 μg/25 mL,加标回收率为94.3%~100.8%.该法用于锌合金中微量镧的测定,方法的相对标准偏差<5.45%,结果满意.

  19. İş-aile çatışmasının iş ve yaşam doyumuna etkilerinin iş stresi ve algılanan sosyal destek açısından otel personeli üzerinde incelenmesi

    Karabacak, Gözde

    2013-01-01

    İş-aile yaşam çatışması (İAYÇ), iş yaşamının aileye, aile yaşamının etkilerinin işe yansımasından kaynaklanan çatışmalardır. İş-aile yaşam çatışmalarının, bireylerin stres seviyesini arttırdığı, stresin de iş ve yaşam doyum düzeylerinde düşüşe neden olduğunu gösteren araştırmalar vardır. Öte yandan birçok araştırmanın sonucu, algılanan sosyal desteğin bireylerin çatışma ve doyum düzeyi ile ilişkili olduğunu göstermektedir. Bu çalışmada amaç, iş stresi, algılanan sosyal destek ve İAYÇ'nin iş v...

  20. Insight into the local magnetic environments and deuteron mobility in jarosite (AFe3(SO4)2(OD)6, A = K, Na, D3O) and hydronium alunite ((D3O)Al3(SO4)2(OD,OD2)6), from variable temperature 2H MAS NMR spectroscopy

    Nielsen, Ulla Gro; Heinmaa, Ivo; Samoson, Ago;

    2011-01-01

    Detailed insight into the magnetic properties and mobility of the different deuteron species in jarosites (AFe3(SO4)2(OD)6, A = K, Na, D3O) is obtained from variable temperature 2H MAS NMR spectroscopy from 40 K to 300 K. Fast MAS results in high resolution spectra of these paramagnetic compounds....... The 2H NMR hyperfine shift (), measured as a function of temperature, provides to be a very sensitive probe of the local magnetic environment. Two different magnetic environments are observed: i) Fe2-OD and D3O+/ in stoichiometric regions of the sample. Here (2H) is proportional to the bulk...... susceptibility and follows a Curie-Weiss law above 150 K. ii) Fe-OD2 and D2O near Fe vacancies. The Fe near these vacancies shows strong local anti-ferromagnetic couplings even high above the Néel temperature (ca. 65 K). 2H NMR can discriminate between D2O and D3O+ ions substituted on the A site due to the...

  1. Un análisis institucional de la implicación del proceso de cambio de los SCG en la evolución de la eficiencia de las PYME An institutional analysis of the implication of the MAS change process for the evolution of SMSC efficiency

    Carlos Eduardo Facin Lavarda

    2009-04-01

    Full Text Available El objetivo general de este trabajo es conocer la relación existente entre el proceso de cambio de un Sistema de Contabilidad de Gestión (SCG en las pequeñas y medianas empresas (PYME y el éxito que genera dicho cambio. El éxito del cambio del SCG se va a medir a través de dos indicadores. El primer indicador, el éxito interno, medirá la distancia que existe entre las expectativas (qué se esperaba de las nuevas técnicas de gestión implantadas y las realidades (qué son. El segundo indicador, el éxito externo, medirá la variación en el nivel de eficiencia relativa de la empresa dentro de su sector. La hipótesis básica de este estudio, plantea que el proceso de cambio puede explicar el nivel de éxito (interno-externo alcanzado en el cambio de un SCG. Para captar y analizar los datos, utilizaremos dos técnicas: el Análisis Envolvente de Datos (DEA y el método del Estudio de Caso (EDC. Los resultados de la investigación permitieron conocer los que factores influyeron, y por tanto explican el éxito de un cambio en los SCG de una PYME del sector de la madera y mueble de la Comunidad Valenciana, España, entre ellos: los conocimientos y habilidades de los agentes implicados en el cambio, la influencia de los expertos externos, la profesionalización de la gestión, los recursos informáticos, la formación interna en SCG, la confianza entre los agentes y la implicación de las personas. Además, hemos observado que los cambios producidos a lo largo de los años analizados influyeron positivamente en el nivel de eficiencia de la organización.This study's general objective is to get to know the relation between the change process of a Management Accounting System (MASO in small and medium-sized companies (SMSC and the success of this change. The success of the MAS change is measured by two indicators. The first, internal success, measures the distance between the expectations (what was expected about the new management techniques

  2. Pazarlama Yeniliği ile Pazarlama Performansı İlişkisini Açıklamaya Yönelik Bir Vaka Çalışması : Süleyman Demirel Üniversitesi Olimpik Yüzme Havuzu Örneği ve Bir Model Önerisi

    Tüfekci, Ömer Kürşad; Tüfekci, Nezihe

    2014-01-01

    Pazarlama yeniliği girişimciliğin bir aracı olarak ele alınabilmektedir. Pazarlama yeniliği yeni süreçler ve farklılık meydana getirmektedir. Bu araştırmada pazarlama yeniliği ile pazarlama performansı ilişkisi açıklanmaya çalışılacaktır. Bu kapsamda Süleyman Demirel Üniversitesi Olimpik Yüzme Havuzunun pazarlama stratejisi ele alınarak bir vaka çalışması yapılmıştır. Araştırmada pazarlama yeniliği değişkenleri olarak ürün, fiyat ve tutundurma faaliyetleri; pazarlama perf...

  3. 企业跨国并购中的技术转移研究——以TCL和万向集团的跨国并购实践为例%Analysis of Technology Transference in Transnational Corporate M&As

    刘文纲; 侯汉坡; 刘春成

    2009-01-01

    Technology transference and spread is one of basic characteristics of the process of transnational corporate M&As, also important source of merger synergy effects, but the process of transference is deeply affected by technology resources' characteristics and culture difference, conflict of strategy. Firstly, this paper analyzed influences of inexplicability and adaptability on the process of transference and spread, and on the basis of the analysis, established a regional model on transnational corporate M&As. Furthermore, this paper analyzed influences of culture difference and conflict of strategy on the process. Afterwards, taking TCL group and Wanxiang group for examples, this paper conducted an empirical analysis of the model.%跨国并购中的技术转移深受技术资源特性、文化差异和竞争战略契合性的影响.分析了隐默性和适配性对技术转移的影响,并建立了技术转移的区域模型;然后,分析了文化差异和竞争战略冲突对跨国并购中技术转移的影响.以TCL集团和万向集团的跨国并购实践为例进行了实证分析.案例研究表明,渐进式的跨国并购值得我国企业重视,即先开展技术贸易,再建立技术联盟,最后实现技术并购.

  4. Development of Dynamically Optimized Wireless Transmission System for Wireless Sensor Networks in Highrise Apartment Building Heating Systems / Optimizētas Dinamiskās Bezvadu Sensoru Tīklu Pārraides Risinājuma Izstrāde Daudzstāvu Ēku Apkures Sistēmas Parametru Izgūšanai Un Vadībai

    Isakovs, V.; Kondratjevs, K.; Kunicina, N.; Freliha, B.; Zabasta, A.

    2015-08-01

    Smart meters will be at the centre of the integrated solutions, including interaction with other networks in the next decade. The developed solution for multi-apartment building ventilation and heating parameter monitoring aims to increase energy efficiency and optimal control of the existing system. Dynamic control of heat and ventilation systems, heat loss detection, calculation and mitigation, and individual heat energy accounting are difficult tasks to accomplish. This article deals with the data transmission system using battery powered ISM band radio transmitters. The temperature measurement sensors with cumulative temperature reading are used as sensor part for this solution. The offered approach to monitoring system is used for overall building and individual apartment monitoring. Data coding and antenna designs are explained for this particular application. Daudzstāvu ēkas, kuras nav sākotnēji plānotas integrācijai ar ēku vadības sistēmām, rada kompleksu problemātisko vidi modernizācijai. Vadu sensoru sistēmu uzstādīšana šādās ēkās kļūst nepraktiska vai pat neiespējama. Šī iemesla dēļ ir nepieciešamas efektīvas bezvadu sensoru sistēmas, kas spētu nodrošināt mērījumu nogādi, ņemot vērā daudzstāvu ēku specifiku. Rakstā tiek piedāvāts bezvadu sensoru tīkla risinājums efektīvai datu pārraidei siltumapgādes sistēmas parametru izgūšanai. Tiek izstrādāta un aprobēta specializētā virziena antena efektīvai radio telegrammu savākšanai no dzīvokļiem, nogādājot mērījumus datu koncentratoros. Ziņojumu telegrammu efektīvai pārraidei tiek piedāvāta datu kodēšanas kumulatīvā metode. Piedāvātais risinājums pielietojams gan jaunām, gan esošām ēkām. Izgūtie dati ir pielietojami siltuma apgādes sistēmas uzraudzībai, regulēšanai, kā arī individuālā siltuma patēriņa tarifikācijai.

  5. Impact of structural differences in carcinopreventive agents indole-3-carbinol and 3,3'-diindolylmethane on biological activity. An X-ray, ¹H-¹⁴N NQDR, ¹³C CP/MAS NMR, and periodic hybrid DFT study.

    Latosińska, Jolanta Natalia; Latosińska, Magdalena; Szafrański, Marek; Seliger, Janez; Žagar, Veselko; Burchardt, Dorota V

    2015-09-18

    Three experimental techniques (1)H-(14)N NQDR, (13)C CP/MAS NMR and X-ray and Density Functional Theory (GGA/BLYP with PBC) and Hirshfeld surfaces were applied for the structure-activity oriented studies of two phyto-antioxidants and anticarcinogens: indole-3-carbinol, I3C, and 3,3'-diindolylmethane, DIM, (its bioactive metabolite). One set of (14)N NQR frequencies for DIM (2.310, 2.200 and 0.110 MHz at 295K) and I3C (2.315, 1.985 and 0.330 MHz at 160K) was recorded. The multiplicity of NQR lines recorded at RT revealed high symmetry (chemical and physical equivalence) of both methyl indazole rings of DIM. Carbonyl (13)C CSA tensor components were calculated from the (13)C CP/MAS solid state NMR spectrum of I3C recorded under fast and slow spinning. At room temperature the crystal structure of I3C is orthorhombic: space group Pca21, Z=4, a=5.78922(16), b=15.6434(7) and c=8.4405(2)Å. The I3C molecules are aggregated into ribbons stacked along [001]. The oxygen atomsare disorderedbetween the two sites of different occupancy factors. It implies that the crystal is built of about 70% trans and 30% gauche conformers, and apart from the weak OH⋯O hydrogen bonds (O⋯O=3.106Å) the formation of alternative O'H⋯O bonds (O'⋯O=2.785Å) is possible within the 1D ribbons. The adjacent ribbons are further stabilised by O'H⋯O bonds (O'⋯O=2.951Å). The analysis of spectra and intermolecular interactions pattern by experimental techniques was supported by solid (periodic) DFT calculations. The knowledge of the topology and competition of the interactions in crystalline state shed some light on the preferred conformations of CH2OH in I3C and steric hindrance of methyl indole rings in DIM. A comparison of the local environment in gas phase and solid permitted drawing some conclusions on the nature of the interactions required for effective processes of recognition and binding of a given anticarcinogen to the protein or nucleic acid. PMID:26066413

  6. Biyoloji Öğrenme Anlayışları Ölçeği’nin (BÖAÖ) Geliştirilmesi: Geçerlilik ve Güvenirlik Çalışması

    TAŞKIN, Nazlı Ruya; Yildirim, Osman; Sami Özgür

    2015-01-01

    Bu çalışmanın amacı 9-12. Sınıflarda öğrenim gören öğrencilerin öğrenme anlayışlarını belirlemek amacı ile bir Biyoloji Öğrenme Anlayışları Ölçeği geliştirmek ve geliştirilen bu ölçekten elde edilen sonuçların geçerlilik ve güvenirlik çalışmasını yapmaktır. Araştırma Balıkesir ili Merkez ilçesindeki devlet okullarında öğrenim gören toplam 997 öğrenciden oluşan iki bağımsız örneklemde gerçekleştirilmiştir. Araştırmanın verileri 41 maddelik 5’li likert tipi ölçekten elde edilmiştir. Açımlayıcı ...

  7. Testēšanas metožu un testēšanas automatizācijas rīku pielietojums tīmekļa bāzētas sistēmas testēšanā

    Jaunošāne, Kristīne

    2016-01-01

    Nav iespējams veikt pilnīgu sistēmas testēšanu, notestēt visas ievadvērtības un nosacījumus, tā vietā jāveic piemērotas testēšanas metodes izvēle, balstoties uz testējamā objekta īpašībām. Bakalaura darbā tiek apskatītas sešas testēšanas metodes, kuras ir aprakstītas ISTQB Foundation Level Syllabus un to praktiska pielietošana tīmekļa bāzētā vietnē, kura tika izstrādāta darba gaitā, vai uz atsevišķi izveidotiem piemēriem. Testēšanas automatizācija var paaugstināt testēšanas efektivitāti un op...

  8. Construindo uma explicação da censura em “Mas apenas e antigamente guirlandas sobre o poço”, de Caio Fernando Abreu = Constructing an explanation of censorship in “But only once and garlands over the pit”, by Caio Fernando Abreu

    Garlet, Deivis Jhones

    2015-01-01

    Full Text Available Considerando a censura como elemento integrante da superestrutura jurídica e política da Ditadura Civil-militar brasileira, propomos uma análise do conto “Mas apenas e antigamente guirlandas sobre o poço”, de Caio Fernando Abreu, o qual foi censurado em 1975. O objetivo consiste em construir, com o recurso de conceitos do Círculo de Bakhtin, como os de reflexo e de refração do fazer artístico e de meio ideológico, juntamente à legislação sobre censura da época, uma explicação dos motivos que podem ter levado ao corte da narrativa e, assim, uma compreensão do pensamento político do autor como um contraponto simbólico ao autoritarismo militar. Desse modo, nosso estudo situa-se no âmbito dos estudos literários de crítica social e de literatura comparada

  9. Kıbrıs Mülkiyet Tartışmasına Taşınmaz Mal Komisyonu Açısından Bir Bakış Cyprus Property Dispute through the Glance of Immovable Property Commission

    Derya ÖZVERİ

    2013-07-01

    Full Text Available The ‘property dispute’ of the Cyprus issue is just one of many layers of the problem. Not only, is it related to the fundamental rights of around 210,000 internally displaced people of the island, but it will also be a focal parameter to economic and social ends in the event of reunification. After presenting a brief historical overview of the problem and the two official positions of both Turkish and Greek sides - the achievement of ‘bizonality’ and the restoration of ‘human rights-, this article seeks to elaborate these two specific positions with a special focus of the cases of Loizidou v. Turkey and Xenides-Arestis v.Turkey in European Court of Human Rights which put the Immovable Property Commission in the focus for the restoration of property claims of displaced people throughout the island. The article argues that the exhaustion of domestic remedies through the Immovable Property Commission could give a new impetus on the negotiation table to the property issue against all odds. Besides this new institutional structure given by the Immovable Property Commission could set a new venue for solving the dispute which has been always in a deadlock for so many years with both parties and with their unarguable positions. Mülkiyet tartışması, Kıbrıs sorununun, çok katmanlı ve yıllardır çözüm bekleyen konularından sadece biri olarak görünse de; ada içinde yerlerinden olmuş 210.000´e yakın kişiyi ilgilendiren ve tüm adanın sosyo-ekonomik ve kültürel yapısını temelinden değiştiren bir olgu olarak karşımıza çıkmaktadır..Mülkiyet tartışmasının çok önemli bir uzlaşmazlık konusu olması ayrıca, adanın tekrar birleşmesi ihtimalinde de bu konunun yine barış masasına yatırılacak ve görüşmeleri kökünden degiştirecek yapıda bır sorun olmasından da kaynaklanmaktadır. Bu çalışma da, mülkiyet tartışmasının adanıngeleceği içindeki önemini, öncelikle tarihsel arka plan

  10. Lınux İşletim Sistemine Karşı Tutum Ölçeği Geliştirme Çalışması/ Developıng Scale For Mesaurıng Students Attıdudes Toward Lınux

    Başer, Mustafa; Dinçer, Serkan

    2013-01-01

    Bu araştırmanın amacı öğrencilerin Linux işletim sistemine karşı tutumlarını ölçen bir ölçek geliştirmektir. Ölçek geliştirilirken daha önce birçok alana uyarlanmış Fennema ve Sherman (1976) tarafından geliştirilen beşli likert tipi ölçek temel alınmış ve konunun gerektirdiği değişiklikler yapılmıştır. Ölçek daha önce Linux işletim sistemi dersini almış toplam 237 öğrenciye uygulanmıştır. Geçerlilik çalışması faktör analizi ile yapılmış ve “Linux'a Değer Verme”, “Linux'da Kendine Güven” ve “S...

  11. Ratio changes of selected components of body composition after lymphatic massage [Změny poměrů vybraných složek tělesného složení po lymfatické masáži

    Jarmila Riegerová

    2009-12-01

    Full Text Available BACKGROUND: The application of lymphatic massages has a highly preventive effect as it reinforces the drainage and transport functions of the lymph pump and thus activates the lymphatic flow and helps detoxify the body. OBJECTIVE: This pilot report inquires into the effect of lymphatic massages on selected components of body composition. METHODS: We conducted an examination of body composition using the InBody 720 device (multifrequency bioelectrical impedance analysis in a population of 30 women before and after having received practical training in lymphatic massages. Our study has pilot features due to professional selection and the low number of participants. We have presented a data analysis which defines the population anthropometrically (in terms of body height and weight and body mass index, waist hip ratio, body fat mass, skeletal muscle mass, rate changes in body water (total body water, intracellular water, extracellular water, proteins, total minerals, bone mineral content and changes to Edema indexes. RESULTS: A significant reduction of total body water (p = 0.042, phi = 0.0 and Edema 2 (p = 0.021, phi = 0.1 was indicated in the massaged area. In the case of Edema 1, significant effects of lymphatic massage were determined in both the right (p = 0.042, phi = 0.4 and the left (p = 0.012, phi = 0.2 upper extremity, and the left lower extremity (p = 0.014, phi = 0.2. Significant differences in Edema 2 were found in both the upper and lower extremities (p < 0.05 between the 1st and 2nd examination. A reduction and equilibration to the standard deviation values was also shown. CONCLUSIONS: Our results suggest the necessity of repeating the experiment in a different time frame. We confirm the positive intervention of lymphatic massage on the interstitium and cellular body environment. [Na dvou výukových kurzech lymfatických masáží jsme ve druhé polovině kurzu provedli vyšetření složení těla přístrojem InBody 720. Vy

  12. MAS architecture and knowledge model for vehicles data communication

    René MANDIAU

    2013-07-01

    Full Text Available Normal 0 21 false false false EN-US JA X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:Cambria; mso-ascii-font-family:Cambria; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Cambria; mso-hansi-theme-font:minor-latin; mso-ansi-language:EN-US;} Completely autonomous vehicles in traffic should allow to decrease the number of road accident victims greatly, and should allow gains in terms of performance and economy. Modelling the vehicles interaction, and especially knowledge sharing, is one of the main challenges to optimize traffic flow with autonomous vehicles. We propose in this paper a model of knowledge communication between mobile agents on a traffic network. The model of knowledge and of interaction enables to propagate new knowledge without overloading the system with a too large number of communications. For that, only the new knowledge is communicated, and two agents communicate the same knowledge only once. Moreover, in order to allow agents to update their knowledge (perceived or created, a notion of degradation is used. A simulator has been built to evaluate the proposal, before to implement it in mobile robots. Some results of the simulator are proposed in this article.

  13. MAS architecture and knowledge model for vehicles data communication

    Emmanuel ADAM

    2012-07-01

    Full Text Available Normal 0 21 false false false EN-US JA X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:Cambria; mso-ascii-font-family:Cambria; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Cambria; mso-hansi-theme-font:minor-latin; mso-ansi-language:EN-US;} Completely autonomous vehicles in traffic should allow to decrease the number of road accident victims greatly, and should allow gains in terms of performance and economy. Modelling the vehicles interaction, and especially knowledge sharing, is one of the main challenges to optimize traffic flow with autonomous vehicles. We propose in this paper a model of knowledge communication between mobile agents on a traffic network. The model of knowledge and of interaction enables to propagate new knowledge without overloading the system with a too large number of communications. For that, only the new knowledge is communicated, and two agents communicate the same knowledge only once. Moreover, in order to allow agents to update their knowledge (perceived or created, a notion of degradation is used. A simulator has been built to evaluate the proposal, before to implement it in mobile robots. Some results of the simulator are proposed in this article.

  14. Cryptanalyse de Schémas Multivariés

    Dubois, Vivien

    2007-01-01

    Multivariate Cryptography can be defined as public key cryptography based on the computational hardness of solving a system of polynomial equations in several variables. Although research on such schemes appeared in the early 80s, it has really been developed over the last ten years, and has given rise to several promising proposals, such as the HFE cryptosystem and the SFLASH signature scheme. Multivariate schemes therefore stand as possible alternatives to the traditional schemes based on p...

  15. CONSUMIDOR DE PRODUTOS VIRTUAIS: USA, MAS NÃO COMPRA

    Jairo Simião Dornelas

    2010-03-01

    Full Text Available Produtos virtuais são aqueles que podem ser enviados do produtor ao consumidor pela Internet. Analisando a experiência da D'Accord Music Software, que está obtendo destaque na venda de software pela Internet, detectou-se um comportamento defensivo dos consumidores para comprar produtos virtuais. Tal comportamento manifesta-se na baixa taxa de conversão entre usuários que testam e os que realmente compram. Este trabalho visa justamente a aprofundar o conhecimento sobre o comportamento de compra do consumidor destes produtos, destacando a adoção do marketing experiencial como resposta aos desafios. A pesquisa foi conduzida através de entrevistas com os empreendedores e a aplicação de um e-survey, com uma amostra de 700 usuários. As conclusões indicam que o fator preço tem maior importância sob a ótica dos consumidores: a virtualidade parece ser de menor relevância. Como resgate do campo, cogita-se que a adoção do marketing experiencial pode ser uma importante estratégia mercadológica para cativar clientes e reduzir seu comportamento defensivo.

  16. Actuarial considerations of medical malpractice evaluations in M&As.

    Frese, Richard C

    2014-11-01

    To best project an actuarial estimate for medical malpractice exposure for a merger and acquisition, a organization's leaders should consider the following factors, among others: How to support an unbiased actuarial estimation. Experience of the actuary. The full picture of the organization's malpractice coverage. The potential for future loss development. Frequency and severity trends. PMID:25647911

  17. "Reposo en cama": algo mas que una sencilla terapia

    Porras Janeth

    1991-12-01

    Full Text Available

    Se ha coincidido en afirmar que es muy agradable y reconfortante caminar tranquilamente en una mañana soleada o hacer ejercicio al aire libre y que esto trae positivos efectos sobre la salud física y mental; como también que es muy agradable tener un período de descanso después de una agotadora jornada de trabajo. Es precisamente esta alternancia entre períodos de actividad y períodos de descanso, lo que permite un óptimo equilibrio fisiológico de cada uno y del conjunto de los sistemas de la economía. Sin embargo, esta no es precisamente la situación de las personas que por diversas situaciones o alteraciones; tales como enfermedades cardiovasculares,  neurológicas, respiratorias, traumas y limitaciones propias de la edad avanzada; voluntaria o involuntariamente disminuyen su actividad física y permanecen medianos o prolongados períodos de reposo en cama; no solamente como ayuda terapéutica para lograr su recuperación, sino como forma de prevenir nuevas crisis o complicaciones.

  18. "reposo en cama": algo mas que una sencilla terapia

    Porras Janeth

    2010-01-01

    Se ha coincidido en afirmar que es muy agradable y reconfortante caminar tranquilamente en una mañana soleada o hacer ejercicio al aire libre y que esto trae positivos efectos sobre la salud física y mental; como también que es muy agradable tener un período de descanso después de una agotadora jornada de trabajo. Es precisamente esta alternancia entre períodos de actividad y períodos de descanso, lo que permite un óptimo equilibrio fisiológico de cada uno y del conjunto de los siste...

  19. An Application of Formal Argumentation: Fusing Bayes Nets in MAS

    Nielsen, Søren Holbech; Parsons, Simon

    2006-01-01

    We consider agents in a multi-agent system, each equipped with a Bayesian network model (BN) of its environment. We want the agents to reach consensus on one compromise network, which may not be identical to a single one of the BNs initially held by the agents, but rather a combination of aspects...... the possible final compromise is a highly complex task. In this work we suggest using formal argumentation as the reasoning mechanism for agents solving this task, and suggest an open-ended agora approach that ensures agents high quality compromises in an anytime fashion....

  20. Mas-Colell Bargaining Set of Large Games

    Massimiliano Amarante; Luigi Montrucchio

    2007-01-01

    We study the equivalence between the MB-set and the core in the general context of games with a measurable space of players. In the first part of the paper, we study the problem without imposing any restriction on the class of games we consider. In the second part, we apply our findings to specific classes of games for which we provide new equivalence results. These include non-continuous convex games, exact non-atomic market games and non-atomic non-exact games. We also introduce, and charac...

  1. An MAS Negotiation Support Tool for Schema Matching

    Nguyen, Quoc Viet Hung; Luong, Hoai Xuan; Miklós, Zoltán; Quan, Thanh Tho; Aberer, Karl

    2013-01-01

    Database schema matching is the process of establishing correspondences between attributes of schemas for data integration purpose. Though various commercial tools have been developed, their results are inherently uncertain. In practice, to obtain correct at-tribute correspondences, there is a need for collecting human input, after the use of automatic matching tools, to reconcile erroneous mappings. We present a negotiation support tool that enables not a single expert but an expert team, wh...

  2. A MAS Negotiation Support Tool for Schema Matching

    Nguyen Quoc Viet, Hung; Xuan Luong, Hoai; Miklos, Zoltan; Aberer, Karl; Tho Quan, Thanh

    2013-01-01

    demo paper International audience Database schema matching is the process of establishing correspondences between attributes of schemas for data integration purpose. Though various commercial tools have been developed, their results are inherently uncertain. In practice, to obtain correct attribute correspondences, there is a need for collecting human input, after the use of automatic matching tools, to reconcile erroneous mappings. We present a negotiation support tool that enables not...

  3. Capitulo XIV. Mas alla de los aparatos mascaras y aparecidos

    Erikson, Philippe

    2014-01-01

    Figura 57 Tépi y Pusapa tocando trompa A menudo Bwishon, amblíope de edad madura, al acunarse suavemente en su hamaca con un niño dulcemente acariciado o retenido a la fuerza en sus brazos, entona el canto de los espíritus ancestrales mariwin: Kanchi buntak, shobo rayon chomebo-o. Mënë bët, Mashë chikeshe-ek. [melodía transcrita por Hélène Erikson] Aún recientemente, antes de la epidemias, existían innumerables coplas de este canto. Hoy en día sólo subsiste este fragmento, lo que ilustra el ...

  4. Norma juht hämas Venemaa osas / Annika Matson

    Matson, Annika, 1976-

    2005-01-01

    Norma juhatuse esimees Peep Siimon teatas osalise tööaja rakendamise vajadust 551 töötaja puhul ning põhjendab seda keerulise olukorraga Venemaal. Diagramm: Vene autoturg kasvab. Vt. samas: Eesti analüütikud osalise tööaja teadet ei pelga. Kommenteerib Valentin Jurkin

  5. Neonatal administration of fluoxetine did not alter the anxiety indicators, but decreased the locomotor activity in adult rats in the elevated plus-maze Administração neonatal de fluoxetina não alterou os indicadores de ansiedade, mas diminuiu a atividade locomotora em ratos adultos no labirinto elevado em cruz

    Valdenilson Ribeiro Ribas

    2008-12-01

    Full Text Available The objective of this study was evaluate the anxiety and locomotor activity (LA in 52 Wistar adult male rats, being 26 treated with fluoxetine (10 mg/Kg - sc in the neonatal period. These same rats received foot shock (FS (1.6-mA - 2-s in the 90th day. The anxiety and LA were appraised by plus-maze. The time spent in the open arms was used as anxiety index and the LA was measured by number of entries in closed arms (NECA and the total of entries (TE. T-test was used with pO objetivo deste estudo foi avaliar a ansiedade e a atividade locomotora (AL em 52 ratos Wistar adultos machos, sendo 26 tratados no período neonatal com fluoxetina (10 mg/Kg - sc e no 90º dia, receberam estímulos elétricos nas patas (1,6-mA-2-s. A ansiedade e a AL foram avaliadas por meio do labirinto elevado em cruz. O tempo de permanência dos animais nos braços abertos (BA foi utilizado como índice de ansiedade e a AL medida pelo número de entradas nos braços fechados (NEBF e pelo total de entradas (TE nos BA e BF. O teste t foi utilizado, com (p<0,05 e os dados apresentados em média±erro padrão. Os animais tratados reduziram o NEBF (2,35±0,33 e o TE (3,96±0,61 comparados a seus controles (4,65±0,52 e (6,96±0,94. A administração neonatal de fluoxetina não alterou a ansiedade, mas diminuiu a AL dos animais que receberam EE.

  6. 血管紧张素-(1-7)/M as 受体轴通过调控 NF-κB 通路保护心肌细胞对抗高糖诱导的损伤%Angiotensin-(1-7)/Mas receptor axis protects cardiomyocytes against high glucose-induced injury by modulating nuclear factor-κB pathway

    梁伟杰; 陈景福; 宋明才; 莫利求; 潘玩莹; 李健豪; 冯鉴强; 张稳柱

    2015-01-01

    AIM:Tostudywhe ther theangiotens in-(1-7)[Ang-(1-7)]/Mas receptor axis protects cardio-myocytes against high glucose (HG)-induced injury by inhibiting nuclear factor-κB (NF-κB) pathway.METHODS:The cell viability was measured by CCK-8 assay.The intracellular levels of reactive oxygen species ( ROS) were detected by DCFH-DA staining .The number of apoptotic cells was tested by Hoechst 33258 nuclear staining .Mitochondrial membrane potential ( MMP) was examined by JC-1 staining.The levels of NF-κB p65 subunit and cleaved caspase-3 protein were de-termined by Western blotting.RESULTS: Treatment of H9c2 cardiac cells with 35 mmol/L glucose (HG) for 30, 60, 90, 120 and 150 min significantly enhanced the levels of phosphorated ( p) NF-κB p65, peaking at 60 min.Co-treatment of the cells with 1 μmol/L Ang-(1-7) and HG for 60 min attenuated the up-regulation of p-NF-κB p65 induced by HG. Co-treatment of the cells with Ang-(1-7) at concentrations of 0.1~30μmol/L and HG for 24 h inhibited HG-induced cy-totoxicity, evidenced by an increase in cell viability .On the other hand, 1 μmol/L Ang-(1-7) ameliorated HG-induced apoptosis, oxidative stress and mitochondrial damage , indicated by decreases in the number of apoptotic cells , cleaved caspase-3 level, ROS generation and MMP loss .However, the above cardioprotective effects of Ang-(1-7) were markedly blocked by A-779, an antagonist of Ang-(1-7) receptor (Mas receptor).Similarly, co-treatment of H9c2 cardiac cells with 100 μmol/L PDTC ( an inhibitor of NF-κB) and HG for 24 h also obviously reduced the above injuries induced by HG.CONCLUSION:Ang-(1-7)/Mas receptor axis prevents the cardiomyocytes from the HG-induced injury by inhibiting NF-κB pathway .%目的:探讨血管紧张素-(1-7)[Ang-(1-7)]/Mas受体轴能否通过抑制核因子-κB (NF-κB)通路对抗高糖( HG)引起的心肌细胞损伤。方法:应用细胞计数检测试剂盒( CCK-8)检测心肌细胞存活率;

  7. TiO{sub 2} colloidal nanocrystals surface modification by V{sub 2}O{sub 5} species: Investigation by {sup 47,49}Ti MAS-NMR and H{sub 2}, CO and NO{sub 2} sensing properties

    Epifani, Mauro, E-mail: mauro.epifani@le.imm.cnr.it [Consiglio Nazionale delle Ricerche—Istituto per la Microelettronica e Microsistemi (CNR–IMM), via Monteroni c/o Campus Universitario, I-73100 Lecce (Italy); Comini, Elisabetta [SENSOR Lab, Department of Information Engineering, Brescia University and CNR-INO, via Valotti 9, 25133 Brescia (Italy); Díaz, Raül [Electrochemical Processes Unit, IMDEA Energy Institute, Avda. Ramón de la Sagra 3, 28935 Móstoles (Spain); Force, Carmen [NMR Unit, Centro de Apoyo Tecnológico, Universidad Rey Juan Carlos, c/Tulipán, s/n, 28933 Móstoles (Spain); Siciliano, Pietro [Consiglio Nazionale delle Ricerche—Istituto per la Microelettronica e Microsistemi (CNR–IMM), via Monteroni c/o Campus Universitario, I-73100 Lecce (Italy); Faglia, Guido [SENSOR Lab, Department of Information Engineering, Brescia University and CNR-INO, via Valotti 9, 25133 Brescia (Italy)

    2015-10-01

    Highlights: • Novel sensing architecture is presented, made by V{sub 2}O{sub 5} modification of TiO{sub 2} surface. • MAS NMR techniques are a powerful tool for studying the influence of the V{sub 2}O{sub 5} layer. • The V{sub 2}O{sub 5} surface deposition enhanced the adsorption properties with respect to pure TiO{sub 2}. - Abstract: TiO{sub 2} and TiO{sub 2}–V{sub 2}O{sub 5} nanocrystals were prepared by coupling sol–gel and solvothermal methods, followed by heat-treatment at 400 °C, after which the mean nanocrystal size was about 5 nm. The materials were characterized by X-ray diffraction, transmission electron microscopy and solid state nuclear magnetic resonance spectroscopy. It was shown that while the TiO{sub 2} phase was always anatase even after heat-treatment at 500 °C, the presence of the vanadium oxide species enhanced the surface re-configuration of the Ti ions. Hence the coordination environment of surface Ti atoms was drastically changed, by formation of further bonds and imposition of a given local geometry. The final hypothesis was that in pure titania surface rearrangement occurs, leading to the new NMR signal, but this modification was favored in the TiO{sub 2}–V{sub 2}O{sub 5} sample, where the Ti surface atoms were forced into the final configurations by the bonding with V atoms through oxygen. The materials heat-treated at 400 °C were used to process chemoresistive sensors, which were tested to hydrogen, CO and NO{sub 2}, as examples of gases with peculiar sensing mechanisms. The results evidenced that the surface deposition of V{sub 2}O{sub 5} onto the anatase TiO{sub 2} nanocrystals was effective in modifying the adsorption properties of the anatase nanocrystals.

  8. Quem sente é a gente, mas é preciso relevar: a lombalgia na vida das trabalhadoras do setor têxtil de Blumenau - Santa Catarina One feels it but one must overlook it: low back pain in the life of women working in the textile sector of Blumenau - Santa Catarina

    Karine Muniz Polizelli

    2010-06-01

    Full Text Available Este estudo foi desenvolvido com o objetivo de compreender o enfrentamento da lombalgia no cotidiano de mulheres trabalhadoras do setor têxtil de Blumenau, Santa Catarina. Três mulheres trabalhadoras do setor têxtil daquela cidade foram as informantes principais do estudo, desenvolvido em encontros quinzenais e visitas a locais de trabalho. Foram utilizados como ferramentas de estudo um calendário com data e dias da semana para a marcação de dores relativas à lombalgia e uma Escala Análogo Visual (EAV. Além de relatos anotados em formulários de acompanhamento dos encontros quinzenais, utilizou-se um diário de campo para transcrição de fatos e relatos. Os dados coletados levaram à construção de duas categorias interpretativas: a dor lombar sob o aspecto da normalidade e a dor sentida. Os resultados da pesquisa levam à conclusão de que a lombalgia, sob o ponto de vista das trabalhadoras do setor têxtil de Blumenau, apresenta-se como uma espécie de conflito entre a dor normal, sem importância social, e a dor sentida e limitante, que traz sofrimentos e angústias no âmbito privado. A dualidade da lombalgia é marcante, pois são mulheres que têm dor, real para elas, com um impacto importante para suas vidas, mas elas mesmas esforçam-se por negligenciá-la, seguindo a concepção socialmente aceita para esta questão no contexto cultural da região. O conceito de lombalgia construído é de algo inerente à vida e ao trabalho, o que não permite o direito de estar doente.This study was developed with the objective of understanding how women working in the textile sector of Blumenau, state of Santa Catarina, cope with low back pain. Three women who worked in the textile sector of that city were the main informants of the study, developed in biweekly meetings and visits to workplaces. The study tools were a calendar with dates and week days, where the participants should mark the occurrence of low back pain, and a Visual Analog

  9. Yeni Harflerin Kabulü Ve Adana Basını’na Yansıması: “Yeni Adana” ve “Türk Sözü” Gazetesi The Acceptance Of New Letters And Reflection Of Them To The Adana Press: “Yeni Adana” And “Türk Sözü”

    Şeyda ÖZÇELİK

    2013-09-01

    Full Text Available The reflection of social, political, cultural, economic and religious developments in a country has been by the media like television, radio, newspaper and magazine. The role of the media in the years of liberation and foundation of Turkish Republic was also very important.Especially the newspapers provided psychological support to the publicin the time of the National Struggle played an active role instrengthening the national feelings, strengthening the unity andsolidarity. The local press as well as the national press informed thepeople of the region and got the people to become conscious about theNational Struggle during the War of Independence. The political andcultural vision of the new regime of Turkish Republic established afterthe War of Independence was involved in national and local mediamoment by moment. The changes and the transformations afterfoundation of state were delivered to the public through the press. Atthis stage, to be understood of Atatürk’s revolutions which were thehistoric moment for the Turkish Republic in becoming contemporarysociety was entrusted a major task of the press. It was the newspapersof the period which was one of the institutions working actively in theannouncement stage of the Letter Revolution one of the revolutions ofAtatürk. The introduction of new letters, the teaching to the public, theopinions of Mustafa Kemal and intellectuals about Latin letters oftenwere been involved in the press. In the study it will benefit from thefunctions of Yeni Adana established in 1918 and was the local media ofAdana and Türk Sözü began broadcasting in 1923. It will be exposedthe announcing of Letter Revolution, introducing of new letters and theroles in the process of implementation of these newspapers. Bir ülkedeki toplumsal, siyasal, kültürel, ekonomik ve dini gelişmelerin halka yansıması televizyon, radyo, gazete, dergi gibi basın yayın araçlarıyla olmuştur. Türkiye Cumhuriyeti Devleti

  10. İlhanlı Hâkimiyeti Altında Anadolu’da Siyasetin Temel Dinamiği: Göçebe Moğol-Türkmen Çatışması The Basic Dynamic Of Politics In Anatolia Under The Ilkhanid Rule: The Struggle Of Nomad Turcomans And Mongols

    Halil ÇETİN

    2012-12-01

    ûk ve Türkiye Selçuklu devletleri ile göçebe Türkmenlerden müteşekkil bir ittifak buldular. Bunlar arasında Türkmenler, Moğolların siyasî amaçlarına ve askerî faaliyetlerine karşı en kararlı direnç noktasını oluşturmuşlardır. Gaza ülküsü gibi dinî motifler de içeren bu direniş, Türkmenlerin Anadolu’yu ısrarlı bir şekilde yurt edinme çabasının bir sonucudur. Esasen Anadolu’nun uç bölgelerine yoğun bir şekilde yerleşen Türkmenler için başka bir seçenek de kalmamıştır. Zira yarımadanın siyasî ve coğrafî sınırları onların bir başka bölgeye göçüne imkân da vermemiştir. Bunun içindir ki Türkmenlerin Anadolu’yu kararlı bir şekilde yurt edinme çabası ile Moğollara ısrarla karşı koymaları birbirini besleyen tavırlardır. Moğol ve Türkmen göçebeleri arasında uzun süren bu çatışmalar, Anadolu’da Selçuklu ve İlhanlı idaresinin çökmesiyle sonuçlanmıştır. Göçebe Türkmenler önde gelen siyasî aktörler olarak neticede Siyasal Türkmen Kimliği edinmişlerdir. XIV. asrın başlarında Anadolu’nun uç bölgelerinde ortaya çıkan Türkmen Beyliklerini kuran siyasî ve toplumsal zümrelerin bu kimliğin etkisinde oldukları görülmektedir. Örneğin Osmanlı Beyliği’nin kurucusu Osman Bey, İlhanlı hâkimiyeti altında Anadolu’da yaşanan Moğol-Türkmen çatışmasının ürünü olan siyasî yöntem, söylem ve araçları kullanmıştır.

  11. " Mi mente decía no... mi cuerpo decía sí...": embarazo en adolescentes escolarizadas Minha mente falava não... mas meu corpo falava sim..." : Gravidez em adolescentes escolarizadas My mind said no... my body said yes...": Pregnancy in school adolescents

    MARÍA ENRIQUETA CORTÉS ORTIZ

    etnografia focalizada, que se realizou durante um ano numa instituição educativa pública do Distrito de Aguablanca em Cali, Colômbia. Participaram como informantes chaves seis adolescentes mães escolarizadas e, como informantes gerais, quinze homens, quinze mulheres não grávidas, dez professores e treze pais de família. A pesquisa inclui entrevistas em profundidade e focus groups até atingir saturação de dados. O método utilizado para a análise foi o método Spradley. A partir da etnosemántica se identificaram quatro domínios relacionados com o gênero, a cultura, a educação e a decisão de ficar grávida. Adicionalmente, e baseado no estudo dos domínios a pesquisa conseguiu identificar e agrupar quatro temas culturais relacionados com o significado de ser mulher durante a adolescência, o estabelecimento das relações de gênero, o planejamento familiar e a realidade ante a decepção dos pais e a frustração do projeto de vida. As e os adolescentes se desenvolvem em contextos culturais de liberalidade, espacialidade e temporalidade que os predispõem e precipitam não só a viver a sexualidade rapidamente, mas a passar do amor romântico ao amor apaixonado.Sexuality, as a very important dimension of the human being, is affected by culture where the meaning and symbols are immersed and which express how adolescents experience it from their cosmovision. This study describes, from the perspective of gender roles, the decision making process in regard to pregnancy of school adolescents. The study was a focused ethnography that was carried out during one year in a public educational institution from the District of Aguablanca in Cali, Colombia. Key informants were six school adolescents who have become mothers and as general informants, fifteen men, fifteen nonpregnant women, ten teachers and thirteen parents. In depth interviews took place and focus groups until data saturation. The Spradley method was used for the analysis. Based on ethnosemantic, four

  12. “Mi mente decía no… mi cuerpo decía sí…”: el camino en decidir embarazarse las adolescentes escolarizadas My mind said no… my body said yes…”: Pregnancy in school adolescents Minha mente falava não… mas meu corpo falava sim…”: Gravidez em adolescentes escolarizadas

    VÁSQUEZ TRUISSI MARTHA LUCÍA

    2009-12-01

    ênero, a cultura, a educação e a decisão de ficar grávida. Adicionalmente, e baseado no estudo dos domínios a pesquisa conseguiu identificar e agrupar quatro temas culturais relacionados com o significado de ser mulher durante a adolescência, o estabelecimento das relações de gênero, o planejamento familiar e a realidade ante a decepção dos pais e a frustração do projeto de vida. As e os adolescentes se desenvolvem em contextos culturais de liberalidade, espacialidade e temporalidade que os predispõem e precipitam não só a viver a sexualidade rapidamente, mas a passar do amor romântico ao amor apaixonado.

  13. Reasons initiation and continue smoking of female university students: A triangulation studyÜniversitedeki kız öğrencilerinin sigara içme davranışını başlatma ve sürdürme nedenleri: Bir triangülasyon çalışması

    Ayşe Koyun

    2015-12-01

    Full Text Available Objective: The aim of this study is to identify the reasons initiate and continue to smoke of female university students’. Method and material: This study is triangulation study. The study conducted in AfyonKocatepe University between May2, 2014 and June 1, 2014. The quantitative study was carried out with 148 female students who smoke selected with cluster-sampling methods. Data were collected using the questionnaire form and Fagerstrom Test for Nicotine Dependence. The quantitative study was carried out two semi structured focus group interviews with 11 female students who smoke. The quantitative data were analysed with descriptive statistics and Chi-square test. The qualitative data were analysed with the Miles and Huberman method. Results: In quantitative study, the average period for students to continue smoking is 3,8±2,4 years. 37,2% of students start smoking cigarettes before they are 18 years old and 12,2% of them smoke more than half a pack. In 36,5% of the students, the reason for initiate to smoke was wannabe and curiosity. In 42,6% of the students, the reason for continue to smoke was addiction/dependency. Students who started smoking before 18 years old, more than 10 cigarettes smoked per day, and living at home have higher nicotine dependence (p<0,05. It is determined that the students who participated in the focus group interviews initiate and continue smoking to cope with stress. Conclusions: The results of the study show that female students initiated smoking with the influence of individuals in the environment and continued smoking due to addiction.   Özet Amaç: Bu çalışmanın amacı üniversitedeki kız öğrencilerin sigara içme davranışını başlatma ve sürdürme nedenlerini belirlemektir. Yöntem: Bu çalışmada bir triangülasyon çalışmasıdır. Çalışma 2 Mayıs - 1 Haziran 2014 tarihleri arasında, Afyon Kocatepe Üniversitesi'nde yürütülmüştür. Nicel çalışma küme örnekleme yöntemi ile se

  14. Ahmet Hamdi Tanpınar'ın Eserlerinde Trajik Olana Yol Açan Ölüm Hayat Çatışması Death-Life Conflict And ‘Tragic’ In The Ahmet Hamdi Tanpınar’s Works

    Tolga BAYINDIR

    2013-07-01

    yanlarını ortaya koyan, onun düşüncelerini derinden etkileyen felsefî bir sorunsaldır. Ruhuyla ölümlü oluşunun farkındalığı, buna rağmen eserleriyle, maziyle, toplumla ve kültürle ölümsüzlüğü isteyişi/arayışı sanatçının eserlerindeki temel çatışma noktalarından birisini oluşturur. Bu çatışmayı yaratan uç değerler yaşam ve ölüm olarak ele alındığında, Ahmet Hamdi Tanpınar'ı kendi "eşiğinde" bulmak mümkün olacaktır. Tanpınar’ın özellikle günlükleri incelendiğinde görülür ki ölüm/ölümlü olma, ölümsüzlük onun yaratma/eser verme eyleminin altında yatan en önemli öğelerden birisidir. Böyle bir durum karşısında Tanpınar, eserlerinde kaçınılmaz olarak bilinç veya bilinçaltı düzeyinde ölüme dair imgeler kullanmaktadır. Yansıttığı bu ölüm anlayışının yanında onun hayata sıkı sıkıya bağlı yanlarını da keşfetmek mümkün hâle gelmektedir. Tanpınar’ın yaşamının son yıllarında ölüm karşısında belirgin bir kaygı duyduğu anlaşılmaktadır. Bu kaygının temelinde onun kendi içinde oluşturduğu ikiliğin sonuçlarını görmek mümkündür. O, dış olarak tanımladığı yaşamında, aslında olmak istediği kişi değildir ve iç dünyasında kurguladığı ben’inin çatışmasını yaşamaktadır. Bu makalede, kendi içinde yaşadığı bu çatışmanın dışa vurumu olarak Tanpınar’ın trajedisini oluşturan ölüm ve yaşam ikilemi üzerinde durulacaktır. Ayrıca, Ahmet Hamdi Tanpınar’ın “ölüm” kavramı üzerindeki düşünceleri temelinde söz konusu kavramın eserlerine yansıyışına farklı bir bakış açısı getirilmeye çalışılacaktır.

  15. Algılanan Örgütsel Desteğin Çalışanların İş-Aile, Aile-İş Çatışması, Örgütsel Özdeşleşme ve İşten Ayrılma Niyetine Etkisi: Savunma Sektöründe Bir Araştırma / The Effect of Perceived Organizational Support on Work-Family Conflict, Family-Work Conflict, Org

    Ömer TURUNÇ; Çelik, Mazlum

    2010-01-01

    Özet: Örgüt başarımında önemli bir role sahip işgücünün beklentileri gün geçtikçe farklılaşabilmektedir. Çalışanlar için örgütsel destek, çalışanların tutumlarında etkisi olan önemli bir faktördür. Örgütsel desteğin çalışanların örgütsel özdeşleşme düzeyine, iş-aile ve aile iş çatışması düzeyi ile işten ayrılma niyetine etkilerini belirlemek bu &...

  16. 新试剂4—MAsA—I和4—MAsA—8Q5S性能研究

    嵇志琴; 黄坚锋

    1993-01-01

    本文详细地研究了试剂4-甲基偶氮胂-I(简称4-MAsA-I)和7-(2-胂酸基-4-甲基)偶氮-8-羟基喹啉-5-磺酸(简称4-MAsA-8Q5S)的光度性能,并将基与二种同类试剂5-羧基偶氮胂-I(简称5-CaAsA-I)和7-(2-胂酸基-5-羧基)偶氮-8羟基喹啉-5-磺酸(简称5-CarAsA-8Q5S)进行了比较,从中找出了一些有关试剂结构与性能之间关系的规律,为今后设计新提供了一定的

  17. 77 FR 43084 - Multiple Award Schedule (MAS) Program Continuous Open Season-Operational Change

    2012-07-23

    ... time for awards, modifications and ] contract options, and improving supplier relationship management..., Assistant Commissioner, Office of Acquisition Management, Federal Acquisition Service, General Services... support by GSA as well as other increased levels of customer support from GSA. Additionally, DBM...

  18. Analisa Timbulan dan Komposisi Sampah Rumah Mewah (Studi Kasus Perumahan Bougenville Panakukkang Mas Makassar )

    Palallo, Madarensiwati

    2014-01-01

    Population growth and changing patterns of comsumption and human lifestyles result in increased trash. Accretion volume and diversity characteristics of waste increasing need good handling and processing to anticipate the problem of waste. Until now, waste management systems, especially in residential areas stiil rely on the ???gather ??? transport - throw???. Public participation is still lacking for the management of waste in an effort to reduce the volume of waste in lanfill (TPA). This s...

  19. Tüüri ava(sta)mas, kuid mitte ainult / Igor Garšnek

    Garšnek, Igor, 1958-

    2011-01-01

    Kahest kontserdist Estonia kontserdisaalis - 4. märtsil ERSO ja Herbert Schuch (klaver) Olari Eltsi dirigeerimisel, kavas Tüüri Sümfoonia nr 8, Pärdi "Nekroloog" ja Ullmanni Klaverikontsert ning 10. märtsil Erkki-Sven Tüüri autorikontsert, kus esinesid Eesti Filharmoonia Kammerkoor ja Sinfonietta Riga Daniel Reussi juhatusel

  20. International Taxation and the Direction and Volme of Cross-Border M&As

    Huizinga, H.P.; Voget, J.

    2006-01-01

    In an international merger or acquisition, the national residences of the acquirer and the target determine to what extent the newly created multinational firm is subject to international double taxation. This paper presents evidence that the parent-subsidiary structure of newly created multinationa

  1. Cholesterol metabolism: increasingly complex; El metabolismo del colesterol: cada vez mas complejo

    Sanhueza, J.; Valenzuela, R.; Valenzuela, A.

    2012-07-01

    Cholesterol is an important molecule; it is necessary for the biosynthesis of steroidal hormones, bile salts and to maintain the stability of biological membranes in animal cells. However, its excess is negative and is responsible for the development of many diseases involving the heart and brain, or in the generation of some types of cancer. For these reasons, the cellular cholesterol levels must be finely regulated and therefore, an infinite number of mechanisms participate in this regulation, which undertake the organism as a whole. These mechanisms should begin to operate efficiently from the intake of cholesterol from the diet, its incorporation into the enterocytes, where are involved carriers such as ABC and NCP1 transporters, PDZ structural motif, to name a few. It is also necessary an adequate regulation of circulating cholesterol and once inside the body, there should be a perfect harmony between the addition of cholesterol to various tissues, its metabolic use, the mechanisms of its tissue deposition, and the synthesis of this lipid. From this perspective, this review offers a general view of the molecular mechanisms that allow the regulation of extra and intracellular cholesterol levels. (Author) 82 refs.

  2. Mergers and Acquisitions (M&AS by R&D Intensive Firms

    Shantanu Dutta

    2009-12-01

    Full Text Available In this study, we evaluate the impact of R&D intensity on acquiring firms’ abnormal returns by examining 925 Canadian completed deals between 1993 and 2002 that have information on R&D expenditures. While examining the returns to acquiring firm shareholders in the R&D intensive firms we evaluate two competing hypotheses: ‘growth potential hypothesis’ and ‘integration failure hypothesis’. According to the ‘growth potential hypothesis’, in light of the growth potential of the targets acquired by R&D intensive firms, investors are likely to react positively. ‘Integration failure hypothesis’ focuses on integration difficulties of a target by an R&D intensive firms and suggests that investor might be skeptical of such acquisitions and react negatively. Our results show that R&D intensity (i.e. R&D expenditure by sales has a positive and significant effect on cumulative abnormal returns of the acquiring firms around the announcement dates. This implies that market generally favors the M&A deals by R&D intensive firms. An analysis of the differentiating characteristics reveal that R&D firms have a significantly higher growth potential and undertake more stock financed deals compared to the non R&D firms. Further, our results show that there is no significant change in long-term operating performance subsequent to the M&A deals for both R&D firms and non R&D firms. In general, our results show support for ‘growth potential hypothesis’.

  3. Universidad, ciencia y desarrollo: mas allá de la línea base

    Orlando Pulido Chaves

    1996-04-01

    Full Text Available Este artículo plantea la hipótesis de que la actual crisis a escala global es producto de un cambio en el modelo de desarrollo del capitalismo, consistente en una transformación sustancial de los procesos productivos, y de la transición hacia una nueva fase del capitalismo, caracterizada por el hecho de que las hegemonías y las supremacías se empiezan a construir con base en el predominio del desarrollo científico y tecnológico. Esta crisis expresa el agotamiento del modelo fordista-taylorista, sustentado en la gran industria y en el control del mercado mundial de bienes manufacturados y de capital, y la aparición de tendencias postfordistas, caracterizadas por la lucha por el control del mercado de servicios y de bienes de alta densidad tecnológica, en el que la producción de conocimiento domina las relaciones comerciales.. En este contexto, la universidad, la educación y la investigación cobran importancia estratégica, y plantean retos decisivos para el futuro de los países subdesarrollados.

  4. The Green Economics in PT Sinar Mas Agro Resources and Technology Tbk (PT SMART Tbk.)

    Muhammad Hakim Haekal; Muhammad Eka Pramudita

    2013-01-01

    Oil palm (Elaeis sp.) is an important industrial plant producing cooking oil (palm oil), the oil industry, as well as fuel (biodiesel), and others. Plantations make a huge profit so many old plantation forest and converted into oil palm plantations in Indonesia. In 2012 Indonesia was the first palm oil producer in the world. Palm oil plantations generate waste from solid to liquid waste. Volume of solid waste in oil palm plantations is quite large, derived from the leaves, stem, and bunches. ...

  5. M\\'as all\\'a del GWAS: alternativas para localizar QTLs

    Biscarini, Filippo; Biffani, Stefano; Stella, Alessandra

    2015-01-01

    Beyond GWAS: alternatives to localize QTLs in farm animals. Two methods that could be used for QTL mapping as alternatives to standard GWAS are presented. The first relies on the differential frequency of runs of homozygosity (ROH) in groups of animals (e.g. cases and controls), while the second stems from resampling techniques used for the prediction of carriers of a mutation, and is based on the frequency of inclusion of polymorphisms (SNP) in the predictive model. ROH were applied to the d...

  6. Registration of retinal images by a MAS-ICP approach — a preliminary study

    Pereira, Carla; Martins, Nelson Costa; Gonçalves, Luís; Ferreira, Manuel João Oliveira

    2012-01-01

    Diabetic retinopathy has been revealed as the most common cause of blindness among people of working age. For monitoring the pathology image registration algorithms applied to retinal images is very useful. In this work, a novel vessel-based retinal image registration approach is proposed. The segmentation of the vasculature is performed by a multi-agent system model. All these information is then used in a Robust Point Matching Iterative Closest Point algorithm improved by a Region Bootstrap...

  7. Industrial Safety. MAS-123. Waste Isolation Division (WID). Management and Supervisor Training (MAST) Program.

    Westinghouse Electric Corp., Carlsbad, NM.

    This learning module, which is part of a management and supervisor training program for managers and supervisors employed at the Department of Energy's Waste Isolation Division, is designed to prepare trainees to promote and monitor the industrial safety program at their plant. The following topics are covered in the module's individual sections:…

  8. Structural characteristics of marine sedimentary humic acids by CP/MAS sup(13)C NMR spectroscopy

    Sardessai, S.; Wahidullah, S.

    Humic acids from sediments of different depositional environments have been studied by solid-state sup(13)C NMR and the results compared with the traditional wet chemical analysis. Results obtained are well in agreement with the previous literature...

  9. Do CEOs trade premiums for personal gain in M&As?

    Qiu, Buhui

    2014-01-01

    markdownabstract__Abstract__ Chief executive officers are not to be fully trusted. At least, says a new study, chief executive officers at companies on the receiving end of a planned merger and acquisition transaction are not to be fully trusted.

  10. 77 FR 58996 - Multiple Award Schedule (MAS) Program Continuous Open Season-Operational Change; Extension of...

    2012-09-25

    ...The General Services Administration (GSA), Federal Acquisition Service (FAS) issued a notice on July 23, 2012. The comment period is extended to provide additional time for interested parties to the review and submit comments on the...

  11. Structural analysis of a signal peptide inside the ribosome tunnel by DNP MAS NMR.

    Lange, Sascha; Franks, W Trent; Rajagopalan, Nandhakishore; Döring, Kristina; Geiger, Michel A; Linden, Arne; van Rossum, Barth-Jan; Kramer, Günter; Bukau, Bernd; Oschkinat, Hartmut

    2016-08-01

    Proteins are synthesized in cells by ribosomes and, in parallel, prepared for folding or targeting. While ribosomal protein synthesis is progressing, the nascent chain exposes amino-terminal signal sequences or transmembrane domains that mediate interactions with specific interaction partners, such as the signal recognition particle (SRP), the SecA-adenosine triphosphatase, or the trigger factor. These binding events can set the course for folding in the cytoplasm and translocation across or insertion into membranes. A distinction of the respective pathways depends largely on the hydrophobicity of the recognition sequence. Hydrophobic transmembrane domains stabilize SRP binding, whereas less hydrophobic signal sequences, typical for periplasmic and outer membrane proteins, stimulate SecA binding and disfavor SRP interactions. In this context, the formation of helical structures of signal peptides within the ribosome was considered to be an important factor. We applied dynamic nuclear polarization magic-angle spinning nuclear magnetic resonance to investigate the conformational states of the disulfide oxidoreductase A (DsbA) signal peptide stalled within the exit tunnel of the ribosome. Our results suggest that the nascent chain comprising the DsbA signal sequence adopts an extended structure in the ribosome with only minor populations of helical structure. PMID:27551685

  12. Classification of astrocyto-mas and meningiomas using statistical discriminant analysis on MRI data

    The objective of this study was to investigate the usefulness of Multivariate Discriminant Analysis for classifying two groups of primary brain tumours, astrocytomas and meningiomas, from Magnetic Resonance Images. Discriminant analysis is a multivariate technique concerned with separating distinct sets of objects and with allocating new objects to previously defined groups. Allocation or classification rules are usually developed from learning examples in a supervised learning environment. Data from signal intensity measurements in the multiple scan performed on each patient in routine clinical scanning was analysed using Fisher's Classification, which is one method of discriminant analysis

  13. Demonstrating multi-layered MAS in control of offshore oil and gas production

    Lindegaard Mikkelsen, Lars; Næumann, J. R.; Demazeau, Y.;

    2013-01-01

    From a control perspective, offshore oil and gas production is very challenging due to the many and potentially conflicting production objectives that arise from the intrinsic complexity of the oil and gas domain. In this paper, we demonstrate how a multi-layered multi-agent system can be used in a...

  14. Datornoziegumi un to kvalifikācijas problēmas

    Aleksejevs, Andris

    2016-01-01

    Maģistra darbā ir analizēta viena no mūsdienu digitālā informācijas laikmeta problēmām – datornoziegumi. Darbā ir apskatīti un definēti datornoziegumi, to atšķirība no citiem informācijas tehnoloģiju noziegumiem, automatizētu datu apstrādes sistēmu saturs, tā papildināšana. Kā arī ir analizētas problemātiskākās noziedzīgā nodarījuma sastāva pazīmes. Tiek dots ieskats tiesu praksē un juridiskajā literatūrā pausto viedokļu analīzē. Darbā ir piedāvāts risinājums ieskatam un priekšlikumiem esoš...

  15. Potensi Fitoplankton sebagai Sumber Daya Pakan pada Pemeliharaan Larva Ikan Mas (Cyprinus carpio di BBPBAT Sukabumi

    Ana Widiana

    2016-03-01

    Full Text Available The main problem encountered in producing larvae  is the larvae  that got low due to high mortality. One attempt to overcome this problem is the use of phytoplankton as natural feed. The purpose of this study is to determine phytoplankton community structure and potential as a resource phytoplankton feed on larval rearing carp. The research was conducted in April 2012. The results showed that the phytoplankton found in the larval rearing pond goldfish are Class Bacillariophyceae (1 genera, Chlorophyceae (13 genera, and Cyanophyceae (7 genera. Phytoplankton Chlorophyceae group is a group that has the highest abundance of larval rearing carp pond. Diversity index of phytoplankton in pond carp larvae ranged from 0.65 to 1.64 (Low Diversity. Based on the preponderance index carp larvae utilize phytoplankton as natural feed by selecting the class Chlorophyceae and Cyanophyceaea in early growth until the time nursery a preponderance index value respectively 87.76% and 12.23%.

  16. SOMOS O QUE FAZEMOS, MAS SOMOS PRINCIPALMENTE, O QUE FAZEMOS PARA MUDAR O QUE SOMOS

    Dalmo Valério Machado De Lima

    2011-07-01

    Full Text Available Ao longo da história, o homem sempre procurou justificar suas ações individuais ou coletivas com base naquilo que poderia ser revertido para o próprio indivíduo ou classe. Nessa esteira, a discussão sobre o conceito e aplicabilidade do conhecimento renderia um debate que transcende os fins a que se destina esse editorial.A fim de demarcar uma linha do tempo e de pensamento ao longo da construção que ora se apresenta não se pode deixar de tomar como referência basilar o grego Platão, para quem o conhecimento consiste de crença verdadeira e justificada. Posteriormente, revendo e aprofundando as lições de seu mestre, Aristóteles desmembra o conhecimento em três áreas: científica, prática e técnica. Na Idade Média, o bom frade Roger Bacon, o maior prócer do chamado empirismo aristotélico, estabeleceu as bases de um tipo de investigação científica ainda hoje vigente centrada no esquema da tentativa e do erro, além de, em suas "horas vagas", travestir-se de propagandista dos "benéficos" efeitos do uso da pólvora no trato dos problemas internos e externos de um reino ou feudo.  Com o surgimento da ciência moderna, entre os séculos XVI e XVII, um período denominado Revolução Científica, sedimentam-se e proliferam as ideias de Isaac Newton, Galileu Galilei, René Descartes, Francis Bacon, Nicolau Copérnico, Louis Pasteur e Francesco Redi entre outros, que, reorganizando o pensamento científico, apontam para um campo novo de conhecimento científico cujas implicações são sentidas até os dias de hoje. 

  17. Izvarošanas kvalifikācijas problēmas

    Nikolajeva, Jūlija

    2016-01-01

    2014.gada 14.jūnijā stājās spēkā Krimināllikuma grozījumi, ar kuriem tika paplašināts izvarošanas jēdziens, kurš šobrīd paredz kriminālatbildību par dzimumaktu, izdarītu pret cietušā gribu, nepielietojot vardarbību, un mainīts cietušā vecums, kas paredzēts Krimināllikuma 159.panta trešajā daļā, no 14 uz 16 gadiem. Izvēlētā temata aktualitāti apstiprina tas, ka noziedzīgo nodarījumu skaits pieaug, pārsvarā tiek izvaroti mazgadīgie un nepilngadīgie, jo viņus ir vieglāk pakļaut izvarotāju ietek...

  18. MAS-based auction for channel selection in mobile cognitive radio networks

    Emna Trigui

    2015-11-01

    Full Text Available Cognitive radio network is a concept of wireless communication for mobile devices that offers the possibility to exploit the unused spectrum resources opportunistically. These networks bring out the need for new solutions that mitigate the spectrum management issue. However, existing works do not focus on devices mobility whereas serious problems arise when users are mobile specifically about their provided quality of services. In this work, we study spectrum sharing and spectrum handoff for mobile secondary users (SUs and we propose a novel approach that can be executed by a mobile SU when traveling through wireless networks. The proposed solution is inspired from multi-agent system auctions and integrates a learning module which accelerates SUs’ spectrum bands allocation. One of the main contributions of this paper is the realistic implementation of the learning based auction and the interesting results obtained through a network discrete event simulator. Results prove that our proposal enhances spectrum utilization and guarantees users satisfaction.  

  19. Türgi euroliidu ukse taha jäämas / Märt Kivine

    Kivine, Märt, 1967-

    2005-01-01

    Üle-euroopalise küsitluse kohaselt on 52% eurooplastest Türgi EL-iga liitumise vastu. Saksa konservatiivide aseesimehe Wolfgang Schäuble on seisukohal, et EL ei tohi laieneda väljapoole Euroopat, vastasel juhul ei samasta inimesed end enam EL-iga. Diagramm: Enamik Türgi liitumise vastu

  20. Main karst and caves of Switzerland; El karst y las cuevas mas importantes de Suiza

    Jeannin, P. Y.

    2016-07-01

    This paper presents an overview of the main karst areas and cave systems in Switzerland. The first part encloses descriptions of the main geological units that hold karst and caves in the country and summarizes a brief history of research and protection of the cave environments. The second part presents three regions enclosing large cave systems. Two regions in the Alps enclose some of the largest limestone caves in Europe: Siebenhengste (Siebenhengste cave system with ∼160 km and Barenschacht with 70 km) and Bodmeren-Silberen (Holloch cave system with 200 km and Silberen System with 39 km). These systems are also among the deepest with depths ranging between 880 and 1340 m. The third example is from the Jura Mountains (northern Switzerland). (Author)

  1. Retrieval of Aerosol Within Cloud Fields Using the MODIS Airborne Simulator (MAS)

    Munchak, L. A.; Levy, R. C.; Mattoo, S.; Patadia, F.; Wilcox, E. M.; Marshak, A.

    2015-12-01

    Passive satellite remote sensing has become essential for obtaining global information about aerosol properties, including aerosol optical depth (AOD) and aerosol fine mode fraction (FMF). However, due to the spatial resolution of satellite aerosol products (typically 3 km and larger), observing aerosol within dense partly cloudy fields is difficult from space. Here, we apply an adapted version of the MODIS Collection 6 dark target algorithm to the 50-meter MODIS airborne simulator retrieved reflectances measured during the SEAC4RS campaign during 2013 to robustly retrieve aerosol with a 500 m resolution. We show good agreement with AERONET and MODIS away from cloud, suggesting that the algorithm is working as expected. However, closer to cloud, significant AOD increases are observed. We investigate the cause of these AOD increases, including examining the potential for undetected cloud contamination, reflectance increases due to unconsidered 3D radiative effects, and the impact of humidification on aerosol properties. In combination with other sensors that flew in SEAC4RS, these high-resolution observations of aerosol in partly cloudy fields can be used to characterize the radiative impact of the "twilight zone" between cloud and aerosol which is typically not considered in current estimates of direct aerosol radiative forcing.

  2. Templo masónico Warwickshire en Edgbaston - Gran Bretaña

    John Madin, Oficina de Proyectos

    1973-03-01

    Full Text Available The new building, which replaces the old one used for the same purpose, is made up of: — The main temple and its dining room. — Seven assembly rooms, with their corresponding dining rooms. — Hall, made up of: dance room; dining room, and ancillary facilities. — Provincial management offices. — Suite for outstanding personalities. — Kitchens, toilets and cloakrooms. — Car park on the site. The building is made of resistant walls of two-face finished brick, made specially for this building, combined on the outside with precast finished concrete components. Its predominating feature is the almost complete lack of windows in the two floors which make it up. Horizontal communications are around an inner hall, and vertical ones through isolated staircases. The whole temple is air-conditioned and artificially lit, the latter being part of the air-conditioning extraction system.La nueva construcción, que sustituye a otra antigua de igual uso, está constituida por: El templo principal y su comedor. Siete salones con sus correspondientes comedores. Salón de festejos, compuesto por: — sala de baile; — comedor, y — servicios auxiliares. Oficinas del administrativo provincial. Suite para personas muy distinguidas. Servicios de cocina, aseos y guardarropa. Aparcamiento en el interior del solar. El edificio está realizado a base de muros resistentes de ladrillo visto a dos caras, fabricado especialmente para esta construcción, combinado en el exterior con elementos prefabricados de hormigón visto. Su característica dominante es la casi total ausencia de ventanas en las dos plantas de que consta. Las comunicaciones horizontales se desarrollan alrededor de un hall interior, y las verticales mediante escaleras aisladas. La totalidad del templo cuenta con servicios de aire acondicionado e iluminación artificial, integrada esta última en el sistema de extracción del aire acondicionado.

  3. Kukurūzų siloso masės panaudojimas biodujų gamyboje

    Ambrazas, Egidijus

    2005-01-01

    The work is directed for one of alternative energy resources – renewable sources of energy. The main task is to examine potential produce biogas out of maize silage. The object of analyses was a biogas plant operated on different organic wastes in the pigs farm of UAB “Lekeciai”. The technologies of biogas production and energy input were analysed in this work. Acording to empirical equations there were developed an estimation methodology of energy consumption in biogas plant. A Energy consum...

  4. Tecnologias mas nuevas para la seguridad escolar (Newer Technologies for School Security). ERIC Digest.

    Schneider, Tod

    This digest in Spanish describes several technologies that can be used to control access to, and improve surveillance of, school grounds. Access can be controlled by using "smart" cards to control keyed entries. Many schools have problems with multiple copies of keys, and these card systems are integrated with computer software that allows for…

  5. T1P(13C) relaxation at high MAS speed and strong spin lock field

    Brus, Jiří

    Brno : Masarykova univerzita, Brno, 1999. s. 46. ISBN 80-210-2053-9. [Spektroskopická konference NMR Valtice /14./. 12.04.1999-14.04.1999, Valtice] R&D Projects: GA AV ČR KSK2050602; GA MŠk ME 097 Subject RIV: CF - Physical ; Theoretical Chemistry

  6. Formal Social Norms and their Enforcement in Computational MAS by Automated Reasoning

    Neruda, Roman; Kazík, O.

    2012-01-01

    Roč. 39, č. 1 (2012), s. 80-87. ISSN 1819-9224 Institutional support: RVO:67985807 Keywords : role model * description logic * integrity constraints * computational intelligence Subject RIV: IN - Informatics, Computer Science

  7. RECICLAJE DE MATERIALES NO FERROSOS, EN BUSCA DE UNA PRODUCCIÓN MAS LIMPIA

    SERGIO AUGUSTO FERNÁNDEZ HENAO; LEONEL ARIAS MONTOYA; LILIANA MARGARITA PORTILLA DE ARIAS

    2010-01-01

    El aluminio y el bronce pertenecen a los materiales no ferrosos y cumplen con la característica de ser cien por ciento reciclables, además el aluminio presenta como ventaja el requerir sólo un cinco por ciento de la energía necesaria para regresar al metal primario inicial. Para producir aluminio a partir del reciclaje de productos usados de dicho metal (reciclaje, escorias, etc), se requiere de procesarlo para recuperar el metal mediante pretratamiento, fundición y refinado. Para proceder...

  8. X-MAS2: Study Systematics on the ICM Metallicity Measurements

    Rasia, E.; Mazzotta, P.; Bourdin, H.; Borgani, S.; Tornatore, L.; Ettori, S.; Dolag, K.; Moscardini, L.

    2007-01-01

    (Abridged)The X-ray measurements of the ICM metallicity are becoming more frequent due to the availability of powerful X-ray telescope with excellent spatial and spectral resolutions. The information which can be extracted from the measurements of the alpha-elements, like Oxygen, Magnesium and Silicon with respect to the Iron abundance is extremely important to better understand the stellar formation and its evolutionary history. In this paper we investigate possible source of bias connected ...

  9. X-MAS2: Study Systematics on the ICM Metallicity Measurements

    Rasia, E; Bourdin, H; Borgani, S; Tornatore, L; Ettori, S; Dolag, K; Moscardini, L

    2007-01-01

    (Abridged)The X-ray measurements of the ICM metallicity are becoming more frequent due to the availability of powerful X-ray telescope with excellent spatial and spectral resolutions. The information which can be extracted from the measurements of the alpha-elements, like Oxygen, Magnesium and Silicon with respect to the Iron abundance is extremely important to better understand the stellar formation and its evolutionary history. In this paper we investigate possible source of bias connected to the plasma physics when recovering metal abundances from X-ray spectra. To do this we analyze 6 simulated galaxy clusters processed through the new version of our X-ray MAp Simulator, which allows to create mock XMM-Newton EPIC MOS1 and MOS2 observations. By comparing the spectroscopic results to the input values we find that: i) Fe is recovered with high accuracy for both hot (T>3 keV) and cold (T5 keV) the spectroscopic measurement may be strongly overestimated up to a factor of 4; iv) Si is well recovered for all th...

  10. 6Li MAS NMR Study of Lithium Insertion into Hydrothermally Prepared Li-Ti-O Spinel

    Krtil, Petr; Dědeček, Jiří; Kostlánová, Tereza; Brus, Jiří

    2004-01-01

    Roč. 7, č. 7 (2004), A163-A166. ISSN 1099-0062 R&D Projects: GA ČR GA203/03/0823 Institutional research plan: CEZ:AV0Z4040901 Keywords : lithium insertion * spinel * NMR Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 2.271, year: 2004

  11. Managing the Evolution of an Enterprise Architecture using a MAS-Product-Line Approach

    Pena, Joaquin; Hinchey, Michael G.; Resinas, manuel; Sterritt, Roy; Rash, James L.

    2006-01-01

    We view an evolutionary system ns being n software product line. The core architecture is the unchanging part of the system, and each version of the system may be viewed as a product from the product line. Each "product" may be described as the core architecture with sonre agent-based additions. The result is a multiagent system software product line. We describe an approach to such n Software Product Line-based approach using the MaCMAS Agent-Oriented nzethoclology. The approach scales to enterprise nrchitectures as a multiagent system is an approprinre means of representing a changing enterprise nrchitectclre nnd the inferaction between components in it.

  12. Quantitative analysis of backbone motion in proteins using MAS solid-state NMR spectroscopy

    Chevelkov, Veniamin; Fink, Uwe; Reif, Bernd [Leibniz-Forschungsinstitut fuer Molekulare Pharmakologie (FMP) (Germany)], E-mail: reif@fmp-berlin.de

    2009-09-15

    We present a comprehensive analysis of protein dynamics for a micro-crystallin protein in the solid-state. Experimental data include {sup 15}N T{sub 1} relaxation times measured at two different magnetic fields as well as {sup 1}H-{sup 15}N dipole, {sup 15}N CSA cross correlated relaxation rates which are sensitive to the spectral density function J(0) and are thus a measure of T{sub 2} in the solid-state. In addition, global order parameters are included from a {sup 1}H,{sup 15}N dipolar recoupling experiment. The data are analyzed within the framework of the extended model-free Clore-Lipari-Szabo theory. We find slow motional correlation times in the range of 5 and 150 ns. Assuming a wobbling in a cone motion, the amplitude of motion of the respective amide moiety is on the order of 10 deg. for the half-opening angle of the cone in most of the cases. The experiments are demonstrated using a perdeuterated sample of the chicken {alpha}-spectrin SH3 domain.

  13. Measuring takeover premiums in cross-border M&As: insights from Turkey

    Arslan, Hasan Burak; Şimşir, Şerif Aziz; Simsir, Serif Aziz

    2014-01-01

    We investigate whether the merger announcement dates provided in a popular Mergers and Acquisitions (M&A) database, SDC, serve as accurate event dates for estimating the wealth effects of mergers on target firms located in Turkey. We find that 74% of SDC’s merger announcement dates are preceded by merger-related events such as merger rumors, target firms’ search for potential acquirers, and early stage merger negotiation announcements. Target cumulative abnormal return (CAR) estimates around ...

  14. La construccion mas local de la seguridad de los alimentos: el caso de la Argentina

    Sozzo, Gonzalo

    2013-01-01

    La cuestión de la seguridad alimentaria como inocuidad constituye un caso particular de construcción de una modalidad de gobierno de un riesgo global: el riesgo alimentario entendido como la probabilidad de sufrir una enfermedad o morir a causa de la ingesta de un alimento.

  15. Nuevas formas de familia, viejas políticas familiares. Mas familias monomarentales

    Susana Ruiz Seisdedos; María del Carmen Martín Cano

    2012-01-01

    En los últimos años la familia tradicional, formada por una pareja heterosexual casada, con o sin descendencia y con los roles de género claramente marcados, ha dejado paso a nuevos modelos de familia, como son matrimonios del mismo sexo, familias recompuestas, familias monoparentales, etc. El objetivo de este artículo es poner de manifiesto este cambio en las relaciones personales y familiares que se está produciendo en España, tomando como referencia uno de los cambios más significativos ex...

  16. Multi-objective integrated process planning and scheduling : a hybrid MAS/ACO approach

    Zhang, Sicheng.; 张思成

    2015-01-01

    Process planning and scheduling are two important manufacturing planning functions which are traditionally performed separately and sequentially. Usually, the process plan has to be prepared first before scheduling can be performed. However, due to the complexity of manufacturing systems and the uncertainties encountered in practical production, the sequentially prepared process plans and schedules may sometimes become inefficient or even infeasible. Integrated process planning and scheduling...

  17. NuMas: A LAN-based materials control and accounting system in production

    A state-of-the-art Nuclear Materials Control and Accounting (NMC and A) System has been implemented and is fully operational at the Paducah Gaseous Diffusion Plant (PGDP) as of September 1994. The uranium enrichment facility is currently regulated by the Department of Energy (DOE) and is in the process of obtaining Nuclear Regulatory Commission (NRC) certification. Implementation of this system has resulted in a tremendous cost savings to the facility as well as improvements to the overall efficiency of the NMC and A department. This paper outlines the benefits of implementing a Personal Computer/Local Area Network (PC/LAN)-based system in hopes of attracting other facilities to explore and utilize its application at their sites

  18. ¿A las puertas de un TLC o mas lejos que nunca?.

    Fander Falconi Benitez.

    2005-06-01

    Full Text Available This article offers a series of reflections concerning the repercussions and implications of the FTA in the specific case of Ecuador. In particular, the author sustains that the agreement cannot be considered simply in terms of trade and that it should be reevaluated in relation to its potential implications for Ecuadorian society as a whole. Within this framework, the text explores the international conditions that have surrounded the negotiation process in order to assess its effects in Ecuador. The author concludes that under the current conditions characterizing the FTA negotiations, the risks of signing the agreement are much greater that the potential benefits of doing so.

  19. Oresteia: Bir Modern Devlet ve Adalet Tartışması

    Arıcı, Oğuz

    2011-01-01

    Oresteia: A Discussion on Modern State and JusticeAbstractThe expressions like ―tribe leader‖, ―tribe state‖ are used as insults in the political discourse of Turkey. These expressions imply that the person or the society which is being addressed are primitive, wild, disordered, in other words barbaric and animal like because they do not have a ―modern state‖. This usage of the term ―tribal leader‖ glorifies a specific kind of political organization –the modern state- on...

  20. Empresa familiar, sim! Mas qual o problema, se a gestão é profissional?

    Alicelia Rosa de Oliveira Pecanha

    2015-07-01

    Full Text Available Este artigo visa contribuir para melhor compreensão da realidade de uma empresa familiar brasileira em fase de transição do seu modelo de gestão familiar para uma gestão profissionalizada. A empresa estudada é uma livraria brasileira de pequeno porte, fundada em 2000, no interior do estado do Rio de Janeiro, atualmente com seis lojas próprias e outra franqueada, totalizando quarenta funcionários. Sua administração, basicamente familiar, necessita de um novo modelo de gestão para acompanhar o ritmo de sua expansão. O método de pesquisa adotado foi o estudo de caso, com a coleta de dados por meio de entrevistas semiestruturadas com os gestores da empresa. Os principais resultados mostraram que, embora a empresa esteja em processo inicial de profissionalização, precisa, no entanto, acelerar esse processo, adotando uma postura proativa que dê suporte ao seu crescimento.

  1. Encefalopatía neonatal. algo mas que asfixia al nacer

    Cifuentes C., Yolanda; Bermúdez, Martha; Arteaga, Clara

    2011-01-01

    La hiperglicinemia no cetósica (HGNC) es un error innatodel metabolismo, de carácter recesivo, debido a un defectoen el sistema de clivaje, que ocasiona acumulaciónde glicina en la sangre y en el sistema nervioso central,donde activa dos receptores diferentes. El receptor localizadoen la médula espinal, inhibitorio, provoca la apneay el hipo de estos pacientes. El receptor cortical,excitatorio, produce la lesión cerebral y las convulsiones.Se presenta la historia de un recién nacido masculino...

  2. Pengaruh Kompensasi Dan Motivasi Terhadap Kinerja Karyawan Pada Pt Ivo Mas Tunggal Sei Rokan.

    Triyono, Muhammad Ghofar

    2015-01-01

    Compensation and motivation is very important in improving the performance of employees. Compensation becomes urge someone unuk carry out an activity to get the best results, work motivation needs to be improved so that employees can carry out a good performance, otherwise the employees who do not have a high motivation to do the job would be difficult to work well and tends not responsible for and do not produktiv even if the employee has a good operational capability. If the work motivation...

  3. Eğitimde Takım Çalışması

    Kocabaş, İbrahim; Fırat Üniversitesi, Teknik Eğitim Fakültesi, Eğitim Bilimleri Bölümü; Gökbaş, Mehmet; İsmet Paşa İlköğretim Okulu

    2003-01-01

    The main aim of this study is to determine the views of principals and teachers about team working. The following were determined: the degree to which principals encouraged team working amongst teachers, the degree to which teachers were enthusiastic about team working, and the levels to which they believed in team working. A relation was found between team working and participation in decision making. Teachers do not agree while principals agree with the view of team leaders are honest and o...

  4. Knowledge plus Attitude in Radiation Protection; Aptitud mas Actitud en Proteccion Radiologica

    Velez, G. R.; Sanchez, G. D.

    2003-07-01

    Since the introduction of the Basic Safety Standards recommendations, the scope of the radiation protection was broadening. On behalf of the incorporation of radiation protection of the patient in medical exposures, the different groups of professionals involved: physicians, medical physicists, radiation protection officers, regulators, etc., have to work together. The objective of radiation protection, that is, to reduces doses from practices, to prevent potential exposures, to detect its occurrence as well as to evaluate and spread such abnormal situations, will be obtained only if it were possible to joint two basic conditions: knowledge and attitude. It should be well known the differences between the backgrounds needed to be for example, a medical physicist or an R.P.O., However, their attitude to solve an eventual problem involving radiation protection should be the same; as well as the behavior of the specialized physician and regulators, in order to add towards common goals. In this work, we show as an example the curricula contents about radiation protection of the cancer of medical physics in the Universidad Nacional de San Martin (UNSAM), and the corresponding module on medical exposures from the Post-Graduate course on Radiation Protection and Nuclear Safety, held since the 80s in Buenos Aires by the National Commission of Atomic Energy, ARN, IAEA, and the Universidad de Buenos Aires. On the other hand, we describe different attitudes which leads or could start major radiological accidents, regardless the level of knowledge in radiation protection. We conclude that the larger numbers of accidents are due to problems in the attitude than in the level of knowledge of the person involved. Consequently; we suggest emphasizing the discussion on how to generate positive attitudes in every professional involucrated, independently of its cognitive profile or level. (Author) 2 refs.

  5. Pengendalian Hama Keong Mas (Pomacea canaliculata Lamarck) Dengan Ekstrak Biji Pinang Pada Tanaman Padi

    Yunidawati, Wiwik

    2012-01-01

    Wiwik Yunidawati, 2012. Pest Control of Golden Snails (Pomacea canaliculata Lamarck) by Areca Extract On Rice Plants. Application of Areca extract can be used to control Golden snail in rice planting. The purpose of this research is to obtain the suitable concentration of Areca extracts to control Golden snail. The research was conducted on December 2010 to April 2011 at the screen house of Observation Laboratory of Plant Disease and Biological Agents and “Balai Benih Induk Murni” Deli Se...

  6. Masáže u psů a koček

    Vasilevová, Lenka

    2013-01-01

    This thesis deals with the issue of massage and other alternative approaches to treatment and prevention of problems in dogs and cats that are, in my opinion, yet breeders and owners of animals underused. The first chapter deals with the development of massages from past to present, then I tried to capture and enumerate devices that are important to massage the animals, describe an environment that is suitable for the positive course of the massage process, list where and who is made to ap...

  7. El Modelo EFQM, mas allá del ISO 9.000

    Carrión García, Andrés

    2006-01-01

    El presente artículo presenta el modelo europeo de gestión de calidad conocido habitualmente como modelo EFQM y se compara con el modelo de gestión de calidad definido en las normas ISO 9000. Se ponen de manifiesto las similitudes y diferencias existentes entre ambos modelos, se resalta la importancia que el modelo EFQM concede a los resultados obtenidos por organización. Se describe también el proceso de autoevaluación mediante el cual las organizaciones controlan situación y progres...

  8. Aspects of Wood Adhesion: Applications of 13C CP/MAS NMR and Fracture Testing

    Schmidt, Robert G

    1998-01-01

    Phenol Formaldehyde (PF) and polymeric isocyanate (pMDI) are the two main types of adhesives used in the production of structural wood-based composites. Much is unknown about various aspects of adhesion between these two types of resins and wood. The present research describes the development of techniques which will permit an enhanced understanding of 1.) the extent of cure of PF within a wood based composite, 2.) the scale of molecular level interactions between PF and pMDI and wood, 3.) ...

  9. Management of MAS by Means of Automated Reasoning in the Role Model. Chapter 13

    Kazík, O.; Neruda, Roman

    Amsterdam: IOS Press, 2012 - (Essaaidi, M.; Ganzha, M.; Paprzycki, M.), s. 309-322. (NATO Science for Peace and Security Series - D: Information and Communication Security. 32). ISBN 978-1-60750-817-5 R&D Projects: GA MŠk OC10047 Institutional research plan: CEZ:AV0Z10300504 Keywords : multi-agent systems * description logic * role model * matchmaking * integrity constraints * automated reasoning * computational intelligence Subject RIV: IN - Informatics, Computer Science

  10. Customer Order Decoupling Point Selection Model in Mass Customization Based on MAS

    XU Xuanguo; LI Xiangyang

    2006-01-01

    Mass customization relates to the ability of providing individually designed products or services to customer with high process flexibility or integration. Literatures on mass customization have been focused on mechanism of MC, but little on customer order decoupling point selection. The aim of this paper is to present a model for customer order decoupling point selection of domain knowledge interactions between enterprises and customers in mass customization. Based on the analysis of other researchers' achievements combining the demand problems of customer and enterprise, a model of group decision for customer order decoupling point selection is constructed based on quality function deployment and multi-agent system. Considering relatively the decision makers of independent functional departments as independent decision agents, a decision agent set is added as the third dimensionality to house of quality, the cubic quality function deployment is formed. The decision-making can be consisted of two procedures: the first one is to build each plane house of quality in various functional departments to express each opinions; the other is to evaluate and gather the foregoing sub-decisions by a new plane quality function deployment. Thus, department decision-making can well use its domain knowledge by ontology, and total decision-making can keep simple by avoiding too many customer requirements.

  11. Contributions aux schémas d'Analyse/Synthèse en Animation par ordinateur

    Courty, Nicolas

    2013-01-01

    Dans le domaine infographie, l'animation par ordinateur désigne notre capacité à faire se mouvoir, via un ensemble de programmes ou d'algorithmes, des représentations géométriques virtuelles d'objets le plus souvent réels. Si les jeux vidéos ou le champ des effets spéciaux sont les premiers domaines d'application, le développement des technologies de l'information dans notre vie de tous les jours a ouvert la voie à un grand nombre d'autres utilisations. L'animation à proprement parler nécessi...

  12. The validity and reliability of motivational factors scale and the benefits scale of participating in trekking activities for Turkish populationBireyleri doğa yürüyüşü yapmaya motive eden faktörler ölçeği ve bu faaliyetlere katılarak elde edilen faydalar ölçeğinin Türk popülasyonu için geçerlilik güvenirlilik çalışması

    Faik Ardahan

    2013-09-01

    Full Text Available There are two main aims of this study; first; define the validity and reliability of “the Motivational Factors Scale for participating in recreational Trekking activities -MFST”; and “the Benefits Scale of participating in Trekking activities - BST”; second; find out the impacts of MFST on BST.As a result, the Motivational Factors Scale for participating recreational Trekking activities, the Benefits Scale of participating in Trekking activities were reliable and valid in the estimation of the motivational factors and benefits of participating in trekking for the Turkish population. ÖzetBu çalışmanın iki temel amacı vardır, ilki; bireyleri “Doğa Yürüyüşü Yapmaya Motive Eden Faktörler Ölçeği-MFST” ve bu faaliyetlere katılarak “Elde Edilen Faydalar Ölçeğinin- BST” Türk popülasyonu için geçerlilik güvenirlilik çalışması yapmak, ikincisi de, MFST ile BST arasındaki ilişkiyi gösterir regresyon modelinin ortaya konulmasıdır. Araştırma tanımlayıcı bir araştırmadır ve bu çalışma sadece doğa yürüyüşçüleri ile sınırlıdır.Araştırma sonucuna göre, MFST’nin bireyi doğa yürüyüşü etkinliklerine motive eden faktörlerin açıklanmasında, BST’nin de bireylerin doğa yürüyüşünden elde ettikleri faydaların açıklanmasında Türk popülasyonu için yeterli oldukları sonucuna varılmıştır.    Neden-Sonuç ilişkisi açısından, Nedenlerin Faydalar üzerine etkileri de araştırmanın temel yapılma gerekçesini açıklamaktadır.

  13. Less and Less for More and More. Economic Organization Booklet 1. Teacher's Edition=Menos y menos por mas y mas. Organizacion economica libro 1. Manual para El Maestro.

    California State Univ., Los Angeles. National Dissemination and Assessment Center.

    The booklet is part of a grade 10-12 social studies series produced for bilingual education. The series consists of six major thematic modules, with four to five booklets in each. The interdisciplinary modules are based on major ideas and are designed to help students understand some major human problems and make sound, responsive decisions to…

  14. Residue specific hydration of primary cell wall potato pectin identified by solid-state 13C single-pulse MAS and CP/MAS NMR spectroscopy

    Larsen, Flemming H; Byg, Inge; Damager, Iben;

    2011-01-01

    hydrate more readily than the galactan side chains and suggests that the overall hydration properties can be controlled by modifying the ratio of these side chains. Enzymatic modification of native (NA) RG-I provided samples with reduced content of arabinan (sample DA), galactan (sample DG), or both side......-galacturonic acid (Rha-GalA) backbone in RG-I. Potential food ingredient uses of RG-I by tailoring of its structure are discussed...

  15. Analýza masážních postupů a účinků sportovní masáže na lidský organismus

    ROULOVÁ, Iveta

    2011-01-01

    At the present hurried time are increasingly interested in increasing the total relaxation and body regeneration in a variety of relaxation and wellness centers through massage. The aim of this thesis on the basis of analysis was a theoretical overview of massage movements, the proper procedure for sorting. There have been capturing the effects that the massage has on the human organism. These effects are complementary and intertwined. They also analyzed different types of massage with a prim...

  16. Socio-demographic and epidemiological characteristics associated with human immunodeficiency virus type I (HIV-1 infection in HIV-1-explosed but uninfected individuals, and in HIV-1-infected patients from a southern brasilian population Características sociodemográficas e epidemiológicas associadas com a infecção pelo vírus da imunodeficiência humana tipo 1 (HIV-1 em indivíduos expostos ao HIV-1 mas não infectados e em pacientes infectados pelo HIV-1, provenientes da população da região Sul do Brasil

    Edna Maria Vissoci Reiche

    2005-10-01

    Full Text Available The ability to control human immunodeficiency virus type 1 (HIV-1 infection and progression of the disease is regulated by host and viral factors. This cross-sectional study describes the socio-demographic and epidemiological characteristics associated with HIV-1 infection in 1,061 subjects attended in Londrina and region, south of Brazil: 136 healthy individuals (Group 1, 147 HIV-1-exposed but uninfected individuals (Group 2, 161 HIV-1-infected asymptomatic patients (Group 3, and 617 patients with AIDS (Group 4. Data were obtained by a standardized questionnaire and serological tests. The age of the individuals ranged from 15.1 to 79.5 years, 54.0% and 56.1% of the Groups 3 and 4 patients, respectively, were men. The major features of groups 2, 3, and 4 were a predominance of education level up to secondary school (55.8%, 60.2% and 62.4%, respectively, sexual route of exposure (88.4%, 87.0% and 82.0%, respectively, heterosexual behavior (91.8%, 75.2% and 83.7%, respectively, and previous sexually transmitted diseases (20.4%, 32.5%, and 38.1%, respectively. The patients with AIDS showed the highest rates of seropositivity for syphilis (25.6%, of anti-HCV (22.3%, and anti-HTLV I/II obtained by two serological screening tests (6.2% and 6.8%, respectively. The results documenting the predominant characteristics for HIV-1 infection among residents of Londrina and region, could be useful for the improvement of current HIV-1 prevention, monitoring and therapeutic programs targeted at this population.Este estudo transversal descreve as principais características sociodemográficas e epidemiológicas associadas com a infecção pelo HIV-1 em 1.061 indivíduos atendidos em Londrina e região, Sul do Brasil: 136 indivíduos saudáveis (Grupo 1, 147 indivíduos expostos ao HIV-1 mas não infectados (Grupo 2, 161 pacientes infectados pelo HIV-1 assintomáticos (Grupo 3 e 617 pacientes com aids (Grupo 4. Os dados foram obtidos pela aplicação de um

  17. Latvijas būvniecības industrijas resursu problēmas vienotā Eiropā

    Skribans, Valerijs

    2009-01-01

    In the paper Latvian construction industry is comprehensively evaluated and compared with the Baltic States and the European Union. Industry resources and it costs are analyzed by group: natural resources, production of building materials, fixed assets and attraction of money capital, personnel costs. Possibilities to obtain profit while working in construction were analyzed. Basing on the analysis, suggestions for improvements in the industry activities are elaborated. Nature factor is one o...

  18. ENCEFALOPATÍA NEONATAL. ALGO MAS QUE ASFIXIA AL NACER Neonathal encefhalopathy something more that perinatal asphysia

    Yolanda Cifuentes C; Martha Bermúdez; Clara Arteaga D

    2007-01-01

    La hiperglicinemia no cetósica (HGNC) es un error innato del metabolismo, de carácter recesivo, debido a un defecto en el sistema de clivaje, que ocasiona acumulación de glicina en la sangre y en el sistema nervioso central, donde activa dos receptores diferentes. El receptor localizado en la médula espinal, inhibitorio, provoca la apnea y el hipo de estos pacientes. El receptor cortical, excitatorio, produce la lesión cerebral y las convulsiones. Se presenta la historia de un recién nacido m...

  19. ENCEFALOPATÍA NEONATAL. ALGO MAS QUE ASFIXIA AL NACER Neonathal encefhalopathy something more that perinatal asphysia

    Yolanda Cifuentes C

    2007-06-01

    Full Text Available La hiperglicinemia no cetósica (HGNC es un error innato del metabolismo, de carácter recesivo, debido a un defecto en el sistema de clivaje, que ocasiona acumulación de glicina en la sangre y en el sistema nervioso central, donde activa dos receptores diferentes. El receptor localizado en la médula espinal, inhibitorio, provoca la apnea y el hipo de estos pacientes. El receptor cortical, excitatorio, produce la lesión cerebral y las convulsiones. Se presenta la historia de un recién nacido masculino, a término, que se torna encefalopático en los primeros días de vida. No presentó hipoglicemia, acidosis metabólica, cetosis ni hiperamonemia. La ecografía transfontanelar fue normal. A los seis días ameritó ventilación mecánica. La cromatografía en capa fina mostró banda de glicina en plasma y orina. El paciente recibió manejo con benzoato de sodio, diazepam y restricción proteica. A los 20 días de vida presentó mejoría neurológica y salió del hospital a los 42 días de vida con igual manejo. El estudio de acilcarnitinas en sangre por tandem-masas hecho en Santiago de Compostela fue normal. La cuantificación de aminoácidos hecha en el CEDEM mostró aumento de los niveles de glicina en plasma y LCR y la relación glicina LCR / plasma confirmó el diagnóstico de hiperglicinemia no cetósica típica. Se revisan las causas de encefalopatía neonatal y se plantea una secuencia para el diagnóstico.Nonketotic hyperglycinemia is an inborn error of metabolism resulting from a defect in the glycine cleavage enzyme system, is inherited as an autosomal recessive trait. It is characterized by elevated concentrations of glycine in blood and central nervous system. Accumulation of glycine in the brain is thought to cause excessive stimulation of the inhibitory and excitatory receptors presenting apnea, hipcus, seizures and brain damage. We report a full-term male newborn with encephalopathy in the first days of life. He did not present hypoglicemia, metabolic acidosis, non hyperammonemia cetosis. The brain ultrasonography was normal. At six days of age he presents respiratory failure and needs mecanic ventilation. Thin layer chromatography shows glycine in plasma and urine. He was treated with sodium benzoate, diazepam and a low-protein diet. He improved and at 42 days of age he was discharged with the same treatment. The acylcarnitine profile does in Santiago de Compostela was normal. The concentrations of amino acids does in CEDEM was found elevated glycine in plasma and cerebrospinal fluid and the glycine ratio LCR/plasma establish the diagnosis of Typical Neonatal non-ketotic hyperglycinemia. We revised the etiology of neonatal encephalopathy and proposed diagnostic secuency.

  20. Minority Shareholders' Wealth Effects and Stock Market Development: Evidence from Increase-in-Ownership M&As

    Petmezas, D

    2010-01-01

    This paper examines, using a global M&A data set, the relationship between the target firm’s minority shareholders’ returns and a country’s stock market development in deals in which large shareholders increase their ownership stakes. For the purpose of this study, we use two measures of stock market development: (1) turnover over GDP, and (2) turnover over market capitalization. We provide evidence supporting the view that minority shareholders in target firms gain significantly more in coun...

  1. Etude de schémas multi-échelles pour la simulation de réservoir

    Ouaki, Franck

    2013-01-01

    In reservoir simulation, rock properties are given by a geological model defined on a grid made of hundreds of thousands of cells. Performing polyphasic flows directly on this grid may require long computation times. Therefore, reservoir engineers usually build upscaled rock properties on a coarser grid and fluid flow simulations are run with this grid. However, with this approach, the influence of the heterogeneities on the flow may be partially lost. Several multiscale methods have been int...

  2. Association analyses of the MAS-QTL data set using grammar, principal components and Bayesian network methodologies

    Karacaören Burak

    2011-05-01

    Full Text Available Abstract Background It has been shown that if genetic relationships among individuals are not taken into account for genome wide association studies, this may lead to false positives. To address this problem, we used Genome-wide Rapid Association using Mixed Model and Regression and principal component stratification analyses. To account for linkage disequilibrium among the significant markers, principal components loadings obtained from top markers can be included as covariates. Estimation of Bayesian networks may also be useful to investigate linkage disequilibrium among SNPs and their relation with environmental variables. For the quantitative trait we first estimated residuals while taking polygenic effects into account. We then used a single SNP approach to detect the most significant SNPs based on the residuals and applied principal component regression to take linkage disequilibrium among these SNPs into account. For the categorical trait we used principal component stratification methodology to account for background effects. For correction of linkage disequilibrium we used principal component logit regression. Bayesian networks were estimated to investigate relationship among SNPs. Results Using the Genome-wide Rapid Association using Mixed Model and Regression and principal component stratification approach we detected around 100 significant SNPs for the quantitative trait (p Conclusions GRAMMAR could efficiently incorporate the information regarding random genetic effects. Principal component stratification should be cautiously used with stringent multiple hypothesis testing correction to correct for ancestral stratification and association analyses for binary traits when there are systematic genetic effects such as half sib family structures. Bayesian networks are useful to investigate relationships among SNPs and environmental variables.

  3. O EWOM sobre a distribuição moderna - Falem bem ou falem mal…mas falem!

    Fonseca, Ana Luísa Rodrigues

    2013-01-01

    A crescente utilização das novas tecnologias, e o desenvolvimento da Internet, com a exponencial utilização dos social media, mudou a forma como as pessoas comunicam entre si, existindo hoje em dia um contexto online rico em informação, o qual se pode traduzir em conhecimento e inteligência, para as empresas que dele decidam usufruir. Na realidade, este contexto originou uma nova possibilidade de monitorização do consumidor e das suas opiniões e interesses, os quais estão reflectidos nos c...

  4. Minha mente falava não… mas meu corpo falava sim…”: gravidez em adolescentes escolarizadas

    CORTÉS ORTIZ, MARÍA ENRIQUETA; VALENCIA MICOLTA, SUSANA GENITH; VÁSQUEZ TRUISSI, MARTHA LUCÍA

    2010-01-01

    La sexualidad, como dimensión trascendental del ser humano, se afecta con la cultura donde están inmersos significados y símbolos que expresan cómo la viven los adolescentes desde su cosmovisión. Esta investigación describe, desde la perspectiva de los roles de género, los procesos de construcción de decisiones frente al embarazo de los adolescentes escolarizados. El estudio fue una etnografía focalizada, que se realizó durante un año en una institución educativa pública del Distrito de Aguab...

  5. Proyecto de instalaciones en la rehabilitación de una Masía en el Maresme

    Pineda Lleó, Luis

    2013-01-01

    Los sistemas constructivos de los años 60 han quedado atrasados en relación a las actuales exigencias normativas. Conceptos como sostenibilidad y eficiencia energética no se tenían en cuenta. En los años 60 las novedades tecnológicas iban enfocadas a la mejora del confort en el uso de la vivienda y no se consideraban aspectos como el impacto ambiental ni las energías renovables. En la actualidad, la concienciación sobre el impacto del ser humano en el medio ambiente ha desencadenado un cam...

  6. Two-dimensional MAS NMR correlation protocols involving double-quantum filtering of quadrupolar spin-pairs.

    Edén, Mattias

    2010-05-01

    Three two-dimensional (2D) NMR homonuclear correlation techniques invoking double-quantum (2Q) filtration of the central transitions of half-integer spins are evaluated numerically and experimentally. They correlate directly detected single-quantum (1Q) coherences in the t(2) domain with either of 1Q, two-spin 2Q or single-spin multiple-quantum coherence-evolutions in the indirect (t(1)) dimension. We employ experimental (23)Na and (27)Al NMR on sodium sulfite and the natural mineral sillimanite (SiAl(2)O(5)), in conjunction with simulated 2D spectra from pairs of dipolar-recoupled spins-3/2 and 5/2 at different external magnetic fields, to compare the correlation strategies from the viewpoints of 2D spectral resolution, signal sensitivity, implementational aspects and their relative merits for establishing internuclear proximities and quadrupolar tensor orientations. PMID:20202872

  7. Programa Jóvenes con Mas y Mejor Trabajo, un análisis sobre su implementación

    Riccio, Natalia

    2014-01-01

    La presente ponencia tiene como objetivo analizar la implementación del Programa Jóvenes con Más y Mejor Trabajo. El mismo surge a partir de mi experiencia laboral dentro del programa dictando talleres1 específicamente en los municipios de Berazategui y Florencio Varela El Programa mencionado, tiene como objetivo “generar oportunidades de inclusión social y laboral de los jóvenes” (art 1, Res. 497/2008, MTE y SS). Esta inclusión es pensada a partir de un conjunto de acciones educativas, de...

  8. Corpos estranhos, mas não esquecidos: representações de mulheres e homens sobre seus corpos feridos

    Evanilda Souza de Santana Carvalho

    2013-02-01

    Full Text Available Estudo qualitativo, cujo objetivo foi apreender e analisar as representações sobre o corpo ferido, e em que foram colhidos, através da entrevista em profundidade, depoimentos de dezoito adultos, feridos crônicos, usuários de ambulatório de cuidado a pessoas com feridas de um hospital público na cidade de Salvador-BA. A partir da Análise de Conteúdo Temática, emergiram as seguintes categorias relativas ao corpo ferido: é um estranho que promove sofrimento; é constantemente vigiado; é rejeitado; é prisioneiro; está vulnerável à violência; exige cuidado especial e, é um corpo em luto. Essas representações firmam-se em imagens negativas e de sofrimento, e evidenciam que o corpo ferido, muito diferente do corpo idealizado, promove sentimentos contraditórios e autodepreciativos. Os resultados revelaram que as pessoas com feridas crônicas estranham seus próprios corpos, experimentam sentimentos negativos acerca de sua imagem, e mobilizam alternativas de autocuidado e de apresentação pessoal, distintas das acionadas antes da cronificação.

  9. Study of metabolic changes in cornea and lens induced by UV radiation and steroids with mas NMR spectroscopy

    Midelfart, A.; Seather, O.; Risa, O.; Krane, J.; Čejková, Jitka

    Geneva : ogranizator symposia, 2002. s. 47. [International congress of eye research /15./. 06.10.2002-10.10.2002, Geneva] R&D Projects: GA MZd NG16 Keywords : UV radiation Subject RIV: FF - HEENT, Dentistry

  10. Mas felicidad para un mayor numero de personas: ¿Es posible esto en México?

    R. Veenhoven (Ruut)

    2013-01-01

    textabstract¿Cuál es el objetivo final de las políticas públicas? Jeremy Bentham (1789) diría: mayor felicidad para más personas. Él entendió la felicidad como el goce subjetivo de la vida, es decir "la suma de placeres y dolores". Sin embargo, en su época la felicidad de la población general no pod

  11. El Material Suplementario En Los Diccionarios Bilingues Espanol-Ingles Mas Representativos De Los Siglos XX y XXI

    Fischer Dorantes, Erica

    2010-01-01

    This work is one of the first attempts to make a detailed and comparative analysis of the supplementary material of several bilingual English-Spanish dictionaries from the 20th and 21st centuries. It examines the historical development of prefaces and appendices in the most representative bilingual English-Spanish dictionaries that have…

  12. Friedel's salt formation in sulfoaluminate cements: A combined XRD and {sup 27}Al MAS NMR study

    Paul, G. [Dipartimento di Scienze ed Innovazione Tecnologica, Università del Piemonte Orientale A. Avogadro, Viale T. Michel 11, 15121 Alessandria (Italy); Boccaleri, E., E-mail: enrico.boccaleri@mfn.unipmn.it [Dipartimento di Scienze ed Innovazione Tecnologica, Università del Piemonte Orientale A. Avogadro, Viale T. Michel 11, 15121 Alessandria (Italy); Buzzi, L.; Canonico, F. [Buzzi Unicem S.p.A., Via L. Buzzi 6, 15033 Casale Monferrato (Italy); Gastaldi, D., E-mail: dgastaldi@buzziunicem.it [Buzzi Unicem S.p.A., Via L. Buzzi 6, 15033 Casale Monferrato (Italy)

    2015-01-15

    Four different binders based on calcium sulfoaluminate cements have been submitted to accelerated chlorination through ionic exchange on hydrated pastes, in order to investigate their ability to chemically bind chloride ions that might reduce chloride penetration. The composition of hydrated cements before and after the treatment was evaluated by means of an X-Ray Diffraction–{sup 27}Al Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy combined study, allowing to take into account even partially amorphous phases and to make quantitative assumption on the relative abundance of the different aluminium-containing phases. It was found that low SO{sub 3} Sulfoaluminate–Portland ternary systems are the most effective in binding chloride ions and the active role played by different members of the AFm family in chloride uptake was confirmed. Moreover, a peculiar behavior related to the formation of Friedel's salt in different pH conditions was also established for the different cements.

  13. Hybridizing cross-polarization with NOE or refocused-INEPT enhances the sensitivity of MAS NMR spectroscopy

    Zhang, Rongchun; Mroue, Kamal H.; Ramamoorthy, Ayyalusamy

    2016-05-01

    Heteronuclear cross polarization (CP) has been commonly used to enhance the sensitivity of dilute low-γ nuclei in almost all solid-state NMR experiments. However, CP relies on heteronuclear dipolar couplings, and therefore the magnetization transfer efficiency becomes inefficient when the dipolar couplings are weak, as is often the case for mobile components in solids. Here, we demonstrate methods that combine CP with heteronuclear Overhauser effect (referred to as CP-NOE) or with refocused-INEPT (referred to as CP-RINEPT) to overcome the efficiency limitation of CP and enhance the signal-to-noise ratio (S/N) for mobile components. Our experimental results reveal that, compared to the conventional CP, significant S/N ratio enhancement can be achieved for resonances originating from mobile components, whereas the resonance signals associated with rigid groups are not significantly affected due to their long spin-lattice relaxation times. In fact, the S/N enhancement factor is also dependent on the temperature, CP contact time as well as on the system under investigation. Furthermore, we also demonstrate that CP-RINEPT experiment can be successfully employed to independently detect mobile and rigid signals in a single experiment without affecting the data collection time. However, the resolution of CP spectrum obtained from the CP-RINEPT experiment could be slightly compromised by the mandatory use of continuous wave (CW) decoupling during the acquisition of signals from rigid components. In addition, CP-RINEPT experiment can be used for spectral editing utilizing the difference in dynamics of different regions of a molecule and/or different components present in the sample, and could also be useful for the assignment of resonances from mobile components in poorly resolved spectra. Therefore, we believe that the proposed approaches are beneficial for the structural characterization of multiphase and heterogeneous systems, and could be used as a building block in multidimensional solid-state NMR experiments.

  14. (14)N overtone NMR under MAS: signal enhancement using symmetry-based sequences and novel simulation strategies.

    Haies, Ibraheem M; Jarvis, James A; Bentley, Harry; Heinmaa, Ivo; Kuprov, Ilya; Williamson, Philip T F; Carravetta, Marina

    2015-03-01

    Overtone (14)N NMR spectroscopy is a promising route for the direct detection of (14)N signals with good spectral resolution. Its application is currently limited, however, by the absence of efficient polarization techniques for overtone signal enhancement and the lack of efficient numerical simulation techniques to aid in both the development of new methods and the analysis and interpretation of experimental data. In this paper we report a novel method for the transfer of polarization from (1)H to the (14)N overtone using symmetry-based R-sequences that overcome many of the limitations of adiabatic approaches that have worked successfully on static samples. Refinement of these sequences and the analysis of the resulting spectra have been facilitated through the development of an efficient simulation strategy for (14)N overtone NMR spectroscopy of spinning samples, using effective Hamiltonians on top of Floquet and Fokker-Planck equations. PMID:25662410

  15. Hydration properties and phosphorous speciation in native, gelatinized and enzymatically modified potato starch analyzed by solid-state MAS NMR

    Larsen, Flemming H.; Kasprzak, Miroslaw Marek; Lærke, Helle Nygaard;

    2013-01-01

    . Comparative analysis of wheat and waxy maize starches demonstrated that starches were similar upon gelatinization independent of botanical origin and that the torsion angles of the glycosidic linkages were averages of the crystalline A and B type structures. In starch suspension phosphorous in immobile...... regions was only observed in NA starch. Moreover phosphorous was observed in a minor pH-insensitive form and as major phosphate in hydrated GEL and BE starches....

  16. Insights into a lipid regulator by solid-state MAS NMR : kinetic and structure-functional studies on diacylglycerol kinase

    Ullrich, Sandra Johanna

    2013-01-01

    In this thesis the integral membrane protein diacylglycerol kinase (DAGK) from E.coli is investigated with solid-state NMR. The aim is to gain an insight into the enzyme’s mechanism through integration of kinetic, structural and dynamic data. The biological function of DAGK is the transfer of the γ-phosphate group from Mg*ATP to diacylglycerol (DAG) building phosphatidic acid (PA)[6] as port of the membrane-derived oligosaccharide cycle[31,34]. Surprisingly, DAGK does not share structural or ...

  17. Yeast-expressed human membrane protein aquaporin-1 yields excellent resolution of solid-state MAS NMR spectra

    Emami, Sanaz; Fan Ying; Munro, Rachel; Ladizhansky, Vladimir; Brown, Leonid S., E-mail: lebrown@uoguelph.ca [University of Guelph, Departments of Physics, and Biophysics Interdepartmental Group (Canada)

    2013-02-15

    One of the biggest challenges in solid-state NMR studies of membrane proteins is to obtain a homogeneous natively folded sample giving high spectral resolution sufficient for structural studies. Eukaryotic membrane proteins are especially difficult and expensive targets in this respect. Methylotrophic yeast Pichia pastoris is a reliable producer of eukaryotic membrane proteins for crystallography and a promising economical source of isotopically labeled proteins for NMR. We show that eukaryotic membrane protein human aquaporin 1 can be doubly ({sup 13}C/{sup 15}N) isotopically labeled in this system and functionally reconstituted into phospholipids, giving excellent resolution of solid-state magic angle spinning NMR spectra.

  18. Yeast-expressed human membrane protein aquaporin-1 yields excellent resolution of solid-state MAS NMR spectra

    One of the biggest challenges in solid-state NMR studies of membrane proteins is to obtain a homogeneous natively folded sample giving high spectral resolution sufficient for structural studies. Eukaryotic membrane proteins are especially difficult and expensive targets in this respect. Methylotrophic yeast Pichia pastoris is a reliable producer of eukaryotic membrane proteins for crystallography and a promising economical source of isotopically labeled proteins for NMR. We show that eukaryotic membrane protein human aquaporin 1 can be doubly (13C/15N) isotopically labeled in this system and functionally reconstituted into phospholipids, giving excellent resolution of solid-state magic angle spinning NMR spectra.

  19. Jaunā ekonomika un jaunie tirgi: pamatprincipi un veidošanās problēmas

    Skribans, V.

    2001-01-01

    In paper are 4 parts: 1) historical economic system review; 2) definition of terms of the new economic; 3) its problems and solving ways overview; 4) part of research method of competition of the traditional and the new economic.

  20. Valen Mas Que Mil Palabras: Las Etiquetas de Advertencia Pictoricas de Tabaco y Los Derechos Linguisticos de EEUU

    Jaron Browne; Susana Hennessey-Lavery; Kim Rogers

    2007-01-01

    Este informe pretende describir el problema fundamental de desigualdad reflejado y sostenido por el uso en EE.UU. de etiquetas sólo de texto y en inglés. Nuestra investigación demuestra una discriminación lingüística innegable en las advertencias de salud sobre el tabaco. El uso de etiquetas de advertencia escritas en inglés y sin dibujos ha creado una grave brecha en la conciencia pública con respecto a los efectos en la salud del fumar para las personas que no hablan inglés o con niveles de...

  1. Sample preparation of membrane proteins suitable for solid-state MAS NMR and development of assignment strategies

    Hiller, Matthias

    2009-01-01

    Although the basic structure of biological membranes is provided by the lipid bilayer, most of the specific functions are carried out by membrane proteins (MPs) such as channels, ion-pumps and receptors. Additionally, it is known, that mutations in MPs are directly or indirectly involved in many diseases. Thus, structure determination of MPs is of major interest not only in structural biology but also in pharmacology, especially for drug development. Advances in structural biology of membrane...

  2. PERBAIKAN MUTU PRODUK GENTENG MELALUI PERBAIKAN BAHAN BAKU (Studi Kasus Di Perusahaan Genteng UD.Tanah Mas Jepara

    Purnawan Adi W

    2012-02-01

    Full Text Available By there is complaint from a part of customer of Jepara roof tile user this time, that is about the product ofless good Jepara roof tile, like the roof tile is mossy easily, fragile, leaky, low compressive strength or it isbroken when it is trampling, the case is back grounded by the raw material and technology are not advanceyet and also process of making roof tile is less perfect.By neglecting available process and technology, the writers try to improve the quality product of Jepara rooftile through the improvement of raw material. By adding the sand to the raw material with composition ofweight comparison 10:1, 5:1, 3:1 (clay : sand and make an experiment directly by definite plasticity, dryand fire shrinkage, compressive strength, porosity and with analysis of variable cost calculation, so it can beconcluded that by adding sand to the raw material with weight comparison 5:1 (clay : sand, it is provenmost exactly in improve or increase the quality product of Jepara roof tile.

  3. Characterizing crystal disorder of trospium chloride: a comprehensive, 13C CP/MAS NMR, DSC, FTIR, and XRPD study

    Urbanová, Martina; Šturcová, Adriana; Brus, Jiří; Beneš, Hynek; Skořepová, E.; Kratochvíl, B.; Čejka, J.; Šeděnková, Ivana; Kobera, Libor; Policianová, Olivia; Šturc, A.

    2013-01-01

    Roč. 102, č. 4 (2013), s. 1235-1248. ISSN 0022-3549 R&D Projects: GA ČR GPP106/11/P426 Institutional support: RVO:61389013 Keywords : trospium chloride * solid state NMR * factor analysis Subject RIV: FR - Pharmacology ; Medidal Chemistry Impact factor: 3.007, year: 2013

  4. Acidic properties of SSZ-33 and SSZ-35 novel zeolites: a complex infrared and MAS NMR study

    Žilková, Naděžda; Gil, B.; Zones, S. I.; Hwang, S.-J.; Voláková, Martina; Čejka, Jiří

    Oxford: Elsevier B.V./Ltd, 2008 - (Gédéon, A.; Massiani, P.; Babonneau, F.), s. 1027-1032. (Studies in surface science and catalysis. Vol. 174). ISBN 978-0-444-53297-8. [International FEZA Conference /4./. Paris (FR), 01.09.2008-04.09.2008] R&D Projects: GA ČR GA104/07/0383; GA AV ČR 1QS400400560 Institutional research plan: CEZ:AV0Z40400503 Keywords : SSZ-33 * SSZ-35 * zeolites * acidity Subject RIV: CF - Physical ; Theoretical Chemistry

  5. Acidic properties of SSZ-33 and SSZ-35 novel zeolites: a complex infrared and MAS NMR study

    Gil, B.; Zones, S. I.; Hwang, S.-J.; Voláková, Martina; Čejka, Jiří

    2008-01-01

    Roč. 112, č. 8 (2008), s. 2997-3007. ISSN 1932-7447 R&D Projects: GA ČR GA104/07/0383; GA AV ČR 1QS400400560 Institutional research plan: CEZ:AV0Z40400503 Keywords : nuclear magnetic resonance * adsorbed probe molecules * angle- spinning NMR Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 3.396, year: 2008

  6. HEASARC Astronomical Archive: GLIESE2MAS - Gliese Catalog Stars with Accurate Coordinates and 2MASS Cross-Identifications

    National Aeronautics and Space Administration — This table contains precise epoch 2000 coordinates and cross-identifications to sources in the 2MASS Point Source Catalog for nearly all stars in the Gliese,...

  7. Structural investigations of PuIII phosphate by X-ray diffraction, MAS-NMR and XANES spectroscopy

    Popa, Karin; Raison, Philippe E.; Martel, Laura; Martin, Philippe M.; Prieur, Damien; Solari, Pier L.; Bouëxière, Daniel; Konings, Rudy J. M.; Somers, Joseph

    2015-10-01

    PuPO4 was prepared by a solid state reaction method and its crystal structure at room temperature was solved by powder X-ray diffraction combined with Rietveld refinement. High resolution XANES measurements confirm the +III valence state of plutonium, in agreement with valence bond derivation. The presence of the americium (as β- decay product of plutonium) in the +III oxidation state was determined based on XANES spectroscopy. High resolution solid state 31P NMR agrees with the XANES results and the presence of a solid-solution.

  8. Structural investigations of PuIII phosphate by X-ray diffraction, MAS-NMR and XANES spectroscopy

    POPA KARIN; RAISON Philippe; MARTEL LAURA; Martin, Philippe; PRIEUR DAMIEN; SOLARI Pier-Lorenzo; BOUEXIERE Daniel; KONINGS Rudy; SOMERS Joseph

    2015-01-01

    PuPO4 was prepared by a solid state reaction method and its crystal structure at room temperature was solved by powder X-ray diffraction combined with Rietveld refinement. High resolution XANES measurements confirm the +III valence state of plutonium, in agreement with valence bond derivation. The presence of the americium (as beta- decay product of plutonium) in the +III oxidation state was determined based on XANES results. High resolution solid state 31P NMR seems to agree with the XANES r...

  9. Účinek kosmetických masáží na vybranou skupinu žen

    Dvořáková, Miroslava

    2015-01-01

    This thesis examines the influence of cosmetic massage to a select group of women, the research relates to the impact of regular massage and treatment on hydration and overall skin condition and cosmetic massage influence on the psychological state of the respondents. The theoretical part defines the concept and importance of massage, composition and function of the skin, explains the process of skin aging. The task of the practical part was to confirm the assumptions that regular cosmetic ma...

  10. Klasická masáž a její modifikace využívané v regeneraci.

    Musilová, Zuzana

    2014-01-01

    The thesis bachelor of two main parts, the first which focuses on massage and its modifications, which are used in regeneration. From the beginning, it is only focused on regeneration and its importance for the various methods of massage, such as Thai massage, honey massage, and others. At the end of the theoretical part focuses on classic massage. The second part focuses on the research part where the task analysis questions and four individual targets, that I had set itself and which I have...

  11. Mediální masáž, aneb, Jsou opravdu defenzivní akcie tak výhodné v dobách krize?

    Vataha, Jiří

    2013-01-01

    The goal of this paper is to refute the fact that it is profitable, in times of economic crisis, or recession, to invest in defensive stocks of anticyclical industries. To refute this fact, I chose the defensive stock of the anticyclical service sector entertainment industry - Walt Disney Co. I will compare the development of these stocks with the developments in the capital market during the economic crisis of 2008/2009. With the aid of correlation and graphical comparisons of the stocks wit...

  12. Hydroprocessing Catalysts. Utilization and Regeneration Schemes Catalyseurs d'hydrotraitement. Schémas d'utilisation et de régénération

    Furimsky E.

    2006-11-01

    Full Text Available The catalyst reactor inventory represents an important part of the cost of hydroprocessing operation. The selection of a suitable catalyst and reactor is influenced by feedstock properties. Processes ensuring an uninterrupted operation during catalyst addition and withdrawal are preferred for processing high asphaltene and metal content feedstocks. The spent catalyst can be regenerated and returned to the operation if the extent of its deactivation is not high. The regeneration may be performed either in-situ or off-site. The former is suitable for fixed bed reactors whereas the catalyst from ebullated bed reactors must be regenerated off -site. The regeneration of spent catalysts heavily loaded with metals such as V, Ni and Fe may not be economic. Such catalysts may be suitable for metal reclamation. An environmentally safe method for catalyst disposal must be found if neither regeneration nor metal reclamation from spent catalysts can be performed. La quantité de catalyseurs utilisée représente une part importante du coût d'une opération d'hydrotraitement. Le choix d'un réacteur et d'un catalyseur approprié dépend des propriétés de la charge. On préfère utiliser les procédés permettant un fonctionnement continu pendant le chargement et le soutirage du catalyseur lorsqu'il s'agit de traiter des charges à haute teneur en asphaltène et en métaux. Le catalyseur usé peut être régénéré et remis en fonctionnement s'il n'est pas trop désactivé. La régénération peut être réalisée in situ ou hors du site. La première solution convient pour les réacteurs à lit fixe, tandis que le catalyseur de réacteurs à lit bouillonnant doit être régénéré hors du site. La régénération de catalyseurs usés fortement chargés en métaux tels que le vanadium, le nickel et le fer n'apparaît pas économique. De tels catalyseurs peuvent convenir pour la récupération des métaux. On doit trouver une méthode sans danger pour l'environnement enfin de se débarrasser du catalyseur dans le cas où on ne peut ni le régénérer ni récupérer les métaux provenant de catalyseurs usés.

  13. Carbonation of C–S–H and C–A–S–H samples studied by 13C, 27Al and 29Si MAS NMR spectroscopy

    Synthesized calcium silicate hydrate (C–S–H) samples with Ca/Si ratios of 0.66, 1.0, and 1.5 have been exposed to atmospheric CO2 at room temperature and high relative humidity and studied after one to 12 weeks. 29Si NMR reveals that the decomposition of C–S–H caused by carbonation involves two steps and that the decomposition rate decreases with increasing Ca/Si ratio. The first step is a gradual decalcification of the C–S–H where calcium is removed from the interlayer and defect sites in the silicate chains until Ca/Si = 0.67 is reached, ideally corresponding to infinite silicate chains. In the seconds step, calcium from the principal layers is consumed, resulting in the final decomposition of the C–S–H and the formation of an amorphous silica phase composed of Q3 and Q4 silicate tetrahedra. The amount of solid carbonates and of carbonate ions in a hydrous environment increases with increasing Ca/Si ratio for the C–S–H, as shown by 13C NMR. For C–A–S–H samples with Ca/Si = 1.0 and 1.5, 27Al NMR demonstrates that all aluminium sites associated with the C–S–H are consumed during the carbonation reactions and incorporated mainly as tetrahedral Al(–OSi)4 units in the amorphous silica phase. A small amount of penta-coordinated Al sites has also been identified in the silica phase

  14. Design of a grounding network in electrical substations. Materials and formulas the most used; Diseno de redes de tierra en subestaciones electricas. Materiales y formulas mas utilizadas

    Raull Martin, J. [Facultad de Ingenieria, UNAM, Mexico, D.F. (Mexico)

    2000-09-01

    The purpose of this paper is to present a summarized version of the different types of construction of grounding systems, as well as their materials and formulas, which are necessary and useful for the design and building of a grounding network for medium to high voltage facilities. A description is also made of how to determine the electrical resistivity of subsoil layers with different physical characteristics. Several illustrative examples are also solved in the paper. [Spanish] El proposito de este articulo es presentar de manera resumida los diferentes tipos de construccion de los sistemas de tierra, asi como sus materiales y formulas, los cuales son necesarios y utiles para el diseno y edificacion de una red de tierra para instalaciones de mediana y alta tension. Se describe tambien la obtencion de las diferentes resistividades que presenta los terrenos con diversas caracteristicas fisicas. Asimismo, se solucionan ejercicios ilustrativos a lo largo del articulo.

  15. Recent Monitoring of Suspended Sediment Patterns along Louisiana's Coastal Zone using ER-2 based MAS Data and Terra Based MODIS Data

    Moeller, Christopher C.; Gunshor, M. M.; Menzel, W. P.; Huh, O. K.; Walker, N. D.; Rouse, L. J.

    2001-01-01

    The University nf Wisconsin and Louisiana State University have teamed to study the forcing of winter season cold frontal wind systems on sediment distribution patterns and geomorphology in the Louisiana coastal zone. Wind systems associated with cold fronts have been shown to model coastal circulation and resuspend sediments along the micro tidal Louisiana coast (Roberts et at. 1987, Moeller et al. 1993). Remote sensing data is being used to map and track sediment distribution patterns for various wind conditions. Suspended sediment is a building material for coastal progradation and wetlands renewal, but also restricts access to marine nursery environments and impacts oyster bed health. Transferring a suspended sediment concentration (SSC) algorithm to EOS MODerate resolution Imaging Spectroradiometer (MODIS; Barnes et al. 1998) observations may enable estimates of SSC globally.

  16. İnternete Yönelik Epistemolojik İnanç Ölçeğinin Uyarlama Çalışması

    Kılıç Çakmak, Ebru; Karaoğlan Yılmaz, F. Gizem; Yılmaz, Ramazan

    2015-01-01

    This study aims to adapt Internet-Specific Epistemological Beliefs Scale, developed by Bråten, Strİmsİ & Samuelstuen (2005) based on Hofer & Pintrich’s (1997) model to survey personal individual beliefs and later revised by Strİmsİ & Bråten (2010), into Turkish and to carry out the reliability and validity analyses of the scale. The study was carried out on 463 university students studying in 2013-2014 spring semester. In the adaptation process of the study, first the language equivalence of ...

  17. Mecanismos, processos e democracias : a sociologia histórica de Charles Tilly e a história breve mas turbulente de um lago

    António Rosas

    2007-01-01

    Full Text Available Compared to its alternatives, the claims that democracies result from the material-economical modernisation, the incarnation of a certain type of political culture or the institutional objectivation seem to be less and less reasonable. The aim of this article is not only to familiarise the reader with one of the most promising theoretical perspectives of contemporary comparative historical sociology – that of Charles Tilly – but also to demonstrate how a processual analysis of political regimes is the more suitable to understand and improve today’s real democracies.

  18. ¿MAS democracia? La democracia en Bolivia y el rol que desempeña el movimiento indígena boliviano

    Olsen, Theis Lundgaard

    2009-01-01

    Specialet omhandler udviklingen af demokratiet i Bolivia og de oprindelige folks bevægelses indflydelse på denne. Dette skal ses i lyset af en øget mobilisering af de oprindelige folk i Latinamerika generelt og mere specifikt ift. Bolivia, at Evo Morales i 2006 blev indsat som den første præsident i landets historie, der er af indiansk afstamning. Den teoretiske ramme udgøres af den franske sociolog Alain Touraines begreber om det "emancipatoriske subjekt" og aktørers "demokratiske ageren". N...

  19. Un critère d'extension d'un foncteur défini sur les schémas lisses

    Guillen, F; Aznar, V Navarro

    1995-01-01

    Let k be a field of characteristic zero. By using Hironaka's desingularisation theorem, we prove an extension criterion for a functor defined on nonsingular k-schemes and taking values on a category of complexes. Roughly speaking, the criterion shows that if such a functor satisfies the standard exact sequence of a blowing-up, then the functor can be extended to all separated k-schemes of finite type. The result is applied to the Grothendieck's theory of motives, to the Hodge-De Rham filtered complex of an analytic space, and to the rational homotopy of k-schemes in algebraic De Rham theory.

  20. PSO based Memetic Algorithms for Product Mix Problems%基于 PSO-MAs 算法的产品组合问题研究

    胡忠义; 鲍玉昆; 熊涛

    2014-01-01

    针对多约束的产品组合问题,提出一种基于PSO的Memetic算法。该算法首先运用约束理论识别并剔除非瓶颈约束,然后基于伪效用比率设计了一个局部搜索算法,并将其加入到PSO算法的种群进化中,以增强PSO算法的局部学习能力。通过对算法在小规模和大规模算例中测试,表明该算法在小规模问题中优于许多已有算法,同时能在相对较短地时间内更有效地求解较大规模产品组合问题。因此本文提出的基于 PSO 的Memetic算法可以用来有效地求解实际中的产品组合问题。%To solve the product mix problem with multiple constraints , a memetic algorithms is proposed based on particle swarm optimization ( PSO) .Firstly, the problem is simplified by recognizing and removing the non-bottle-necks based on the Theory of Constraints ( TOC) .Secondly , a pseudo utility ratio based local search is proposed to improve the exploitation ability of PSO .Both small-scale benchmark datasets and a group of randomly genera-ted large-scale examples are used to test the proposed approach on solving the product mix problems .The compu-tational results show that the proposed approach outperform some existing approaches , such as TOC , revised TOC, Tabu Search(TS), Simulated Annealing(SA)and Genetic Algorithms(GA), and can solve the large-scale problems more effectively .Hence, the proposed approach can be accepted as a practical approach to solve the product mix problem .

  1. Etkin Piyasalar Hipotezi ve Davranışsal Finans Çatışması(A Conflict Beetween The Efficient Market Hypothesis and Behavioral Finance

    İhsan Kulali

    2016-03-01

    Full Text Available Etkin piyasalar hipotezi (EPH, çoğu finansal iktisatçı tarafından yaygın şekilde kabul görmektedir. Modeli savunanlar sermaye piyasalarının, hisse senedi fiyatlarının ilgili elde edilebilir tüm bilgileri yansıtması nedeniyle, etkin olduğuna inanmaktadır. Yeni bir bilgi ortaya çıktığı zaman, haberler anında yayılmakta ve hisse senedi fiyatlarına gecikmeksizin yansımaktadır. Bu görüşe göre hisse senetlerinin gelecek fiyatlarının tahmini için teknik ve temel analize ihtiyaç bulunmamaktadır çünkü hiçbir yatırımcının piyasayı yenmesi ve aşırı kar elde etmesi mümkün değildir. Model, bütün yatırımcıların rasyonel ve faydalarını maksimize etmeye çalışan kişiler olduğunu varsaymaktadır. Yirminci yüzyılın başlarında davranışsal finans gündeme gelmiştir. Birçok finansal iktisatçı hisse senedi fiyatlarının en azında belirli ölçülerde tahmin edilebilir olduğuna inanmaktadır. Pek çok piyasa anomalisi ise psikolojik ve davranışsal faktörler ile açıklanmaktadır. Davranışsal finans yatırımcıları rasyonel değil normal olarak kabul etmektedir. Buna göre piyasa balonları ve krizler bilişsel ön yargılardan kaynaklanmaktadır.

  2. Zaļā publiskā iepirkuma tiesiskā regulējuma aktuālās problēmas

    Šmagre, Asnāte

    2016-01-01

    Maģistra darbs sniedz ieskatu par zaļo publisko iepirkumu, tā nepieciešamību un organizēšanu Latvijā un Eiropas Savienībā. Zaļais publiskais iepirkums tā sabiedriskās un valstiskās nozīmības dēļ Latvijā ir viens no aktuālākajiem jautājumiem un diskusiju objektiem. Saskaņā ar Eiropas Savienības nostādnēm par ilgtspējīgas un gudras sabiedrības un ekonomikas attīstības stratēģiju, zaļais publiskais iepirkums ir viena no prioritātēm dabas resursu saglabāšanai tagadnē un nākotnē. Eiropas Savienīb...

  3. Investigation of Pozzolanic Reaction in Nanosilica-Cement Blended Pastes Based on Solid-State Kinetic Models and 29Si MAS NMR

    Jiho Moon

    2016-02-01

    Full Text Available The incorporation of pozzolanic materials in concrete has many beneficial effects to enhance the mechanical properties of concrete. The calcium silicate hydrates in cement matrix of concrete increase by pozzolanic reaction of silicates and calcium hydroxide. The fine pozzolanic particles fill spaces between clinker grains, thereby resulting in a denser cement matrix and interfacial transition zone between cement matrix and aggregates; this lowers the permeability and increases the compressive strength of concrete. In this study, Ordinary Portland Cement (OPC was mixed with 1% and 3% nanosilica by weight to produce cement pastes with water to binder ratio (w/b of 0.45. The specimens were cured for 7 days. 29Si nuclear magnetic resonance (NMR experiments are conducted and conversion fraction of nanosilica is extracted. The results are compared with a solid-state kinetic model. It seems that pozzolanic reaction of nanosilica depends on the concentration of calcium hydroxide.

  4. El crecimiento económico en América Latina y sus perspectivas mas allá del sexenio perdido

    Hubert Escaith

    2004-01-01

    Full Text Available La volatilidad que caracterizó a la coyuntura regional e internacional en los últimos quince años dificulta pronosticar el crecimiento de América Latina en el mediano plazo. La ausencia de un modelo aceptado universalmente para explicar el crecimiento económico complica aún más esta tarea. El trabajo analiza diversos enfoques y metodologías para medir de manera empírica las tendencias de fondo del crecimiento regional, identificar particularismos subregionales y extrapolar el potencial de crecimiento económico. Se destaca el impacto de las reformas estructurales en la productividad total de factores y el crecimiento potencial, al efecto de arrastre del comercio externo y a la sustentabilidad de estrategias de apertura económica.

  5. "Quien Sabe Mas Lucha Mejor": Adult Educators' Care of the Self Practices within Social Movements in Buenos Aires, Argentina

    O'Donnell, Jennifer Lee

    2014-01-01

    This article looks at popular adult educators' care of the self practices within social movements in Buenos Aires, Argentina. It answers the following questions: How is popular adult education practiced amongst educators in social movements? What can studying popular adult educators' care of the self practices offer the field of adult…

  6. Lizbon Antlaşması Sonrası Avrupa Birliği'nin Yeni Kurumsal Yapısı

    CALISKAN, Ozgur

    2011-01-01

    The Lisbon Treaty entered into force on December 1, 2009, marking a new level of EU’s initiatives on institutional change and policy reforms in 2000’s. The purpose of this study is to provide an outline of the changes in the institutional structure of EU with the Lisbon Treaty, considering the targets of reform initiatives started in the late 1990’s. The study argues that although the Treaty ensures new institutional innovations and designs, the question of whether those reforms would be suff...

  7. PEMBERIAN TOTAL FENOL TERIPANG PASIR (Holothuria scabra UNTUK MENINGKATKAN LEUKOSIT DAN DIFERENSIAL LEUKOSIT IKAN MAS (Cyprinus carpio YANG DIINFEKSI BAKTERI Aeromonas Hydrophila

    Achmad Suhermanto

    2011-10-01

    Full Text Available Freshwater fishery commodities are most likely to be increased production of carp (Cyprinus carpio. Intensification of cultivation which raises new problems with fish disease outbreaks caused by Aeromonas hydrophila. Providing of immunostimulatory bioactive compounds of sea cucumbers (Holothuria scabra can enhance non-specific immune responses in the goldfish as a mechanism of defense against disease. The purpose of this study is to determine the role and optimal dose of total phenols sea cucumber in enhancing non-specific immune response seen from the hematology parameters Extraction of bioactive components from sea cucumber prepared by using methanol and fractionated with ethyl acetate solvent (v/v. Identification of total phenols in ethyl acetate fraction performed using UV-Vis spectrophotometer and infrared. Total phenol produced was tested on carp with intraperitonial injection, the concentration of 0.09, 0.18 and 0.27 mg phenol/kg fish. Challenge test was done by using the Aeromonas hydrophila (107 cells/ml through the immersion method. The observations included hematological parameters and total plasma proteins.The observations of hematological parameters that Leukocytes, Neutrophils pre-and post-infection were significantly increased. Lymphocyte pre infection was increased significantly and post-bacterial infection was decreased significantly. Eosinophils, Monocytes pre-and post-bacterial infection were not differ significantly between treatment. The use of phenolic compounds at 0.09 mg / kg can be increase of non-specific immune response in carp (Cyprinus carpio. Key Words : Total phenol, Aeromonas hydrophila, Cyprinus carpio, nonspecific immune response 

  8. Influence of Modified Atmosphere Packaging on Storability and Postharvest Quality of Cornelian Cherry (Cornus mas L.) Fruits

    Sheida MOHEBBI; Younes MOSTOFI; Zabihallah ZAMANI; Najafi, Farzaneh

    2015-01-01

    Cornelian cherries were stored in two types of polymeric films (low density polyethylene and polypropylene) and three gas combinations 5%O2 + 20%CO2 + 75%N2, 60%O2 + 20%CO2 + 20%N2 and Air) at 1 °C and 90-95% relative humidity for 35 days. Unpackaged cornelian cherries were used as a control. Samples were taken initially and at intervals of 7-days during storage, when quality parameters were measured. Results showed that modified atmosphere packaging (MAP) allowed the stored fruits to retain ...

  9. Aprovechamiento solar en los terrenos agrarios de la Masía Can Gili, término municipal de Barberá del Vallés

    Seven Porte, Xavier

    2012-01-01

    El objeto del presente proyecto define los conceptos y desarrolla cálculos necesarios para llevar a cabo el aprovechamiento energético mediante el uso de energías renovables, en nuestro caso la energía solar. La naturaleza de la energía solar proviene de esa gigantesca esfera formada de gases a alta temperatura. La temperatura en su interior se estima que es de 107 K, en su superficie oscila entre los 5762 y los 6300 K. El Sol genera energía mediante reacciones nucleares de ...

  10. Mujer Mas Segura (Safer Women: a combination prevention intervention to reduce sexual and injection risks among female sex workers who inject drugs

    Vera Alicia

    2012-08-01

    Full Text Available Abstract Background Female sex workers who inject drugs (FSW-IDUs are at risk of acquiring HIV, sexually transmitted infections (STI and blood-borne infections through unprotected sex and sharing injection equipment. We conducted a 2×2 factorial randomized controlled trial to evaluate combination interventions to simultaneously reduce sexual and injection risks among FSW-IDUs in Tijuana and Ciudad Juarez, Mexico. Methods/design FSW-IDUs ≥18 years reporting sharing injection equipment and unprotected sex with clients within the last month were randomized to one of four conditions based on an a priori randomization schedule, blinding interviewer/counselors to assignment. Due to the extreme vulnerability of this population, we did not include a control group that would deny some women access to preventive information. All women received similar information regardless of group allocation; the difference was in the way the information was delivered and the extent to which women had an interactive role. Each condition was a single 60-minute session, including either an interactive or didactic version of an injection risk intervention and sexual risk intervention. Women underwent interviewer-administered surveys and testing for HIV, syphilis, gonorrhea, Chlamydia, and Trichomonas at baseline and quarterly for 12 months. Combined HIV/STI incidence will be the primary outcome. Secondary outcomes are proportionate reductions in sharing of injection equipment and unprotected sex with clients. Discussion Of 1,132 women, 548 (48.4% were excluded (88.9% were ineligible; 11.1% refused to participate or did not return; 584 eligible women enrolled (284 in Tijuana; 300 in Ciudad Juarez. All 584 participants completed the baseline interview, provided biological samples and were randomized to one of the four groups. During follow-up, 17 participants (2.9% were lost to follow-up, of whom 10 (58.8% had died, leaving 567 participants for analysis. This study appears to be the first intervention to attempt to simultaneously reduce injection and sexual risk behaviors among FSW-IDUs. The factorial design will permit analysis to determine whether the combination of the two interactive interventions and/or its respective components are effective in reducing injection and/or sexual risks, which will have direct, tangible policy implications for Mexico and potentially other resource-poor countries. Trial registration NCT00840658

  11. Approaching the young generation. How to transfer knowledge to future professionals; Acercarse a los mas jovenes. Como transmitir el conocimiento a los futuros profesionales?

    Garcia Laruelo, J.; Vinusa Carretero, A.

    2016-08-01

    One of the main goals of Spanish Young Generation Network (in Spanish Jovenes Nucleares) is the dissemination of nuclear science and technology knowledge to the students and general public. From this point of view, we approach the younger students, our future professionals, in order to teach them this science and to answer the main questions society has about this sector. (Author)

  12. Roy Armes, Les cinémas du Maghreb, trad. de l’anglais par Françoise Rippe-Lascout, Marie-Cécile Wouters.

    Trémel, Laurent

    2012-01-01

    Publié dans la collection « Images plurielles », proposant face à la « menace de standardisation occidentale » (voir texte de présentation de la collection) une réflexion sur « les scènes et les écrans œuvrant de par le monde » (ibid.), cet ouvrage, traduit de l’anglais, est l’œuvre de Roy Armes, Professeur émérite à la Middlesex University de Londres, auteur d’une vingtaine de livres sur le cinéma. Le volume se compose de dix chapitres (pp. 21-186), rassemblés en deux parties : « Historique ...

  13. Effects of the time of cuttings collection and IBA concentration on the rooting of softwood cuttings from elite trees of Cornelian cherry (Cornus mas L. in Belgrade area

    Marković Marija

    2014-01-01

    Full Text Available In this study, the effect of concentration of indole-3-butyric acid (IBA (powder dip, cutting type as well as the time of taking cuttings on the rooting of softwood cuttings of cornelian cherry was examined. Four types of cuttings were used: basal cuttings, terminal cuttings, basal cuttings with 2-year-old wood and terminal cuttings with 2-year-old wood. The obtained results showed that IBA concentration, cutting type and time of collecting have significant effect on rooting. The best results were obtained using 1% IBA and cuttings collected in the second term (in mid-July had a higher rooting percentage. Terminal cuttings treated with 1% IBA (powder dip should be used for optimum results. In that case the rooting percentage was very high (over 90% in both terms of cuttings collection. [Projekat Ministarstva nauke Republike Srbije, br. 43007: Istraživanje klimatskih promena na životnu sredinu: praćenje uticaja, adaptacija i ublažavanje

  14. Matematik İnanç Ölçeği’nin Türkçe’ye Uyarlama Çalışması

    Haciömeroğlu, Yrd.doç. Dr.Güney

    2013-01-01

    Purpose of this study is to examine the reliability and validity of the Turkish adaptation of Mathematical Belief Instrument MBI that was developed by Peterson Fennema Carpenter and Loef 1989 This instrument was adapted to Turkish in order to determine elementary preservice teachers’ mathematical beliefs Data gathered from 301 elementary preservice teachers were used for Exploratory Factor Analysis EFA and Confirmatory Factor Analysis CFA to determine the structure of factor loadi...

  15. Investigating sorption on iron-oxyhydroxide soil minerals by solid-state NMR spectroscopy: a 6Li MAS NMR study of adsorption and absorption on goethite

    Nielsen, Ulla Gro; Paik, Younkee; Julmis, Keinia;

    2005-01-01

    system representing an ideal model system for NMR studies. 6Li resonances with large hyperfine shifts (approximately 145 ppm) were observed above the goethite point of zero charge, providing clear evidence for the presence of Li-O-Fe connectivities, and thus the formation of an inner sphere Li+ complex...... on the goethite surface. Even larger Li hyperfine shifts (289 ppm) were observed for Li+-exchanged goethite, which contains lithium ions in the tunnels of the goethite structure, confirming the Li assignment of the 145 ppm Li resonance to the surface sites. Udgivelsesdato: 2005-Oct-6...

  16. Carbonation of C–S–H and C–A–S–H samples studied by {sup 13}C, {sup 27}Al and {sup 29}Si MAS NMR spectroscopy

    Sevelsted, Tine F.; Skibsted, Jørgen, E-mail: jskib@chem.au.dk

    2015-05-15

    Synthesized calcium silicate hydrate (C–S–H) samples with Ca/Si ratios of 0.66, 1.0, and 1.5 have been exposed to atmospheric CO{sub 2} at room temperature and high relative humidity and studied after one to 12 weeks. {sup 29}Si NMR reveals that the decomposition of C–S–H caused by carbonation involves two steps and that the decomposition rate decreases with increasing Ca/Si ratio. The first step is a gradual decalcification of the C–S–H where calcium is removed from the interlayer and defect sites in the silicate chains until Ca/Si = 0.67 is reached, ideally corresponding to infinite silicate chains. In the seconds step, calcium from the principal layers is consumed, resulting in the final decomposition of the C–S–H and the formation of an amorphous silica phase composed of Q{sup 3} and Q{sup 4} silicate tetrahedra. The amount of solid carbonates and of carbonate ions in a hydrous environment increases with increasing Ca/Si ratio for the C–S–H, as shown by {sup 13}C NMR. For C–A–S–H samples with Ca/Si = 1.0 and 1.5, {sup 27}Al NMR demonstrates that all aluminium sites associated with the C–S–H are consumed during the carbonation reactions and incorporated mainly as tetrahedral Al(–OSi){sub 4} units in the amorphous silica phase. A small amount of penta-coordinated Al sites has also been identified in the silica phase.

  17. Örgütsel Çekicilik : Bir Ölçek Uyarlama Çalışması

    Seda DURAL

    2014-12-01

    Full Text Available Bu çalışmanın amacı, Highhouse vd. (2003 tarafından geliştirilen Örgütsel Çekicilik Ölçeğini (ÖÇÖ Türkçe’ye kazandırmak ve geçerlik ve güvenirlik analizlerini yapmaktır. Ölçek, öncelikle araştırmacılar ve bir dil uzmanı tarafından Türkçe’ye çevrilmiş, ardından ifadeler iki dilli 15 uzmanın görüşüne başvurularak dil ve anlam uygunluğu bakımından değerlendirilmiştir. Öneriler doğrultusunda son şeklini alan Türkçe form iki aşamada uygulanmıştır. İlk aşamada, test-tekrar test güvenirliğini sınamak amacıyla ölçek iki hafta ara ile 50 öğrenciye uygulanmıştır. Test tekrar-test güvenirlik katsayısı birincil değerlendirme için 0,85, ikincil değerlendirme için 0,89 olarak bulunmuştur. Uygulamanın ikinci aşamasında anket 257 öğrenciye uygulanarak ölçeğin iç tutarlılık katsayısı 0,89 olarak bulunmuştur. Yapılan analizler sonucunda ÖÇÖ’nün özgün ölçekte olduğu gibi üç faktörlü bir yapı gösterdiği belirlenmiştir. Hiçbir madde atılmadan 15 madde ile üç boyutu ölçen geçerli ve güvenilir bir ölçek elde edilmiştir.

  18. Gastronomi Turizminin Şehir Markalaşmasına Etkisi: Afyonkarahisar İli Örneği

    Aydın, Ebru

    2015-01-01

    Gastronomi turizmi, temel motivasyon faktörü olan özel bir yemek türünü tatmak veya bir yemeğin üretilmesini görmek amacıyla, yiyecek üreticilerini, yemek festivallerini, restoranları ve özel alanları ziyaret etmektir. Bununla birlikte yemeklerin farklı üretim süreçlerini görmek veya ünlü bir şefin elinden yemek yeme yine bu kapsamda ele alınmaktadır. Gastronomi şehirler için farkındalık yaratan bir unsurdur. Günümüzde rekabet yalnızca ürünler veya firmalar arasında değil ülkeler ve kentl...

  19. El cuerpo: silencioso pero significativo en expresión O corpo: silencioso mas significativo em expressão The body: silent but meaningful in expression

    Martha Cecilia Madrigal Ramírez

    2011-07-01

    Full Text Available Investigación en salas de espera de servicios de urgencias de la ciudad de Medellín, Colombia. Objetivo: identificar las maneras en que las personas expresan sus emociones durante la espera en los servicios de urgencias. Metodología: enfoque cualitativo, con herramientas de la etnografía focalizada; se realizaron 50 horas de observación. Análisis a partir de diarios de campo y entrevistas; se extractaron códigos que se agruparon en categorías para obtener una visión total del fenómeno; se garantizó la confidencialidad. Resultados y discusión: las salas de espera de los servicios de urgencias son espacios impersonales que limitan la comunicación y el acompañamiento entre las personas, y hacen que se generen diferentes estados emocionales que se expresan a través del cuerpo. Se suscitan conversaciones con extraños, movimientos, miradas y gestos con diferentes significados. Reflexión: el conocimiento de las diferentes formas de expresión de las experiencias emocionales de los pacientes y sus familias permite implementar, en las instituciones de salud, mecanismos para favorecer la comunicación asertiva entre usuarios y personal de salud, para la toma de decisiones oportunas y adecuadas a cada situación, de manera que la atención de urgencias sea un proceso humanizado.Pesquisa feita nas salas de espera dos serviços de emergência da cidade de Medellín, Colómbia. Objetivo: Identificar as formas de expressão das emoções pessoais durante a espera nos serviços de emergência. Metodologia: Abordagem qualitativa, com instrumentos saídos da etnografia focalizada. Foram realizadas 50 horas de observações. Analisaramse os diários de campo e entrevistas, e códigos foram extraídos e foram agrupados em categorias para obter uma visão global do fenómeno; garantindo sempre a confidencialidade. Resultados e discussão: as salas de espera dos serviços de emergência são espaços impessoais que limitam a comunicação e isolam as pessoas. Isto produz diferentes estados emocionais que se expressam no corpo. Pessoas desconhecidas conversam e se produzem inúmeros movimentos, olhares e gestos de diferentes significados. Reflexão: O conhecimento das diferentes formas de expressão das emoções dos pacientes e das famílias deles possibilitam a implementação, nos institutos de saúde, de mecanismos que favoreçam a comunicação assertiva entre os usuários e os profissionais da saúde, visando tornar a atenção de emergência um processo humanizado.Research conducted in waiting rooms of emergency services in the city of Medellín, Colombia. Purpose: to identify ways in which people express emotions during waiting periods in the emergency services. Methods: qualitative approach with focalized ethnography tools; 50 observation hours were used. Analysis from field logs and interviews; codes were extracted and grouped in categories to obtain a total view ofthe phenomenon; confidentiality was guaranteed. Results and Discussion: waiting rooms of emergency services are impersonal spaces which limit communication and accompaniment between people and generate different emotional states which are expressed through the body. Conversations with strangers, movements, looks and gestures with different meanings appear. Thoughts: knowledge of the different forms of expression of emotional experiences of patients and their families allows implementing, in health institutions, mechanisms to favor assertive communication between users and the personnel to make appropriate and adequate decisions for each situation, thus making emergency care a humanized process.

  20. El cuerpo: silencioso pero significativo en expresión O corpo: silencioso mas significativo em expressão The body: silent but meaningful in expression

    Martha Cecilia Madrigal Ramírez; Marta Adíela Lopera Betancur; Constanza Forero Pulido; Lucelly García Henao

    2011-01-01

    Investigación en salas de espera de servicios de urgencias de la ciudad de Medellín, Colombia. Objetivo: identificar las maneras en que las personas expresan sus emociones durante la espera en los servicios de urgencias. Metodología: enfoque cualitativo, con herramientas de la etnografía focalizada; se realizaron 50 horas de observación. Análisis a partir de diarios de campo y entrevistas; se extractaron códigos que se agruparon en categorías para obtener una visión total del fenómeno; se gar...

  1. Yüz ve Boynun Önemli Vasküler Anomalileri-Klinik Önemleriyle Birlikte Bir Kadavra Çalışması

    Vollala, Venkata Ramana; BOLLA, Sreenivasa Rao; PAMİDİ, Narendra

    2008-01-01

    Objectives: The objective of this study was to find out vascular variations in the face and neck region. Materials and Methods: 75 cadavers of both sexes have been used in the current study. Head and neck dissections of these cadavers were done in the department of anatomy, Kasturba Medical College and Melaka Manipal Medical College, Manipal. Results: Out of 75 cadavers, we observed five variations in four different cadavers, the findings include; facial vein continuing as external jugular ...

  2. Hydroprocessing Catalysts. Utilization and Regeneration Schemes Catalyseurs d'hydrotraitement. Schémas d'utilisation et de régénération

    Furimsky E.

    2006-01-01

    The catalyst reactor inventory represents an important part of the cost of hydroprocessing operation. The selection of a suitable catalyst and reactor is influenced by feedstock properties. Processes ensuring an uninterrupted operation during catalyst addition and withdrawal are preferred for processing high asphaltene and metal content feedstocks. The spent catalyst can be regenerated and returned to the operation if the extent of its deactivation is not high. The regeneration may be perform...

  3. Azoto ir sieros poveikis vasarinių kviečių sausosios masės prieaugiui ir lapų plotui

    Šiaudinis, Gintaras; Lazauskas, Sigitas

    2006-01-01

    Pastaraisiais dešimtmečiais Lietuvoje padažnėjusių sausringų laikotarpių įtaka vasarinių kviečių produktyvumui, ypač mitybos azotu ir siera aspektu, mažai tyrinėta. Azoto ir sieros įtaka vasarinių kviečių sausųjų medžiagų ir lapų ploto prieaugiui tirta 2003-2005 m. Lietuvos žemdirbystės institute. Lauko bandymai atlikti pagal dviejų veiksnių schemą, kurioje buvo trys tręšimo azotu (0, 60, 120 kg ha-1) bei trys tręšimo siera (0, 15, 30 kg ha-1) lygiai. Vasarinių kviečių vegetacijos metu kas sa...

  4. Rear-end Alarm Model Based on MAS and Driver Behavior%基于MAS和驾驶员行为的追尾预警模型

    梁军; 陈小波; 程显毅; 李小燕

    2009-01-01

    针对现有汽车追尾预警系统以车间距离测量作为碰撞发生的唯一决定因素的不足,提出基于MAS和驾驶员行为的追尾预警模型(MRWMB).采用MAS技术和基于Bayes决策的追尾预警算法,实现追尾碰撞概率的实时计算.仿真结果证明,MRWMB模型在预警的自主性和抗干扰方面比传统的预警系统有明显优势,预警平均性能提高10%左右.

  5. highFAR建筑设计生成方法探索%Exploration of Generative Method Based on MAS for Architectural Design: highFAR

    李飚; 钱敬平

    2011-01-01

    highFAR生成工具是东南大学建筑学院2007年毕业班建筑生成设计研究组的生成设计教学系列探索之一.通过highFAR基于多智能体系统数字生成工具的程序开发研究,尝试高层建筑高密度布局的新途径,为同类建筑应用程序开发提供必要的算法参考.%This paper presents one of the teaching explorations of Generative Design Group ( 2007 ) in School of Architecture. Southeast University. Based on the multi-agent systems and computer programming, the group explores a new method of layout of high-rise building with high-density by digital too! named highFAR. HighFAR provides necessary references tor the development of congener architectural application software.

  6. Elemental and structural analysis of silicon forms in herbal drugs using silicon-29 MAS NMR and WD-XRF spectroscopic methods.

    Pajchel, L; Nykiel, P; Kolodziejski, W

    2011-12-01

    The objective of this work was to study concentration of silicon and its structural forms present in herbal drugs. Equisetum arvense and Urtica dioica L. from teapot bags, dietary supplements (tablets and capsules) containing those herbs, dry extract obtained from a teapot bag of E. arvense, and samples of the latter herb harvested in wild habitat over four months were studied using wavelength dispersive X-ray spectroscopy (WD-XRF) and high-resolution solid-state (29)Si NMR. The highest concentration of Si, ca. 27mg/g, was found in the herbal material from the teapot bags containing E. arvense. The Si content in natural E. arvense (whole plants) increased from May to August by ca. 7mg/g, reaching value 26mg/g. Three different silicon forms were detected in the studied herbal samples: Si(OSi)4 (Q(4)), Si(OH)(OSi)3 (Q(3)) and Si(OH)2(OSi)2 (Q(2)). Those sites were populated in E. arvense in the following order: Q(4)≫Q(3)>Q(2). A dramatic, ca. 50-fold decrease of the Si concentration during the infusion process was observed. The infusion process and the subsequent drying procedure augmented population of the Q(4) sites at the cost of the Q(2) sites. The WD-XRF and (29)Si NMR methods occurred useful and complementary in the study of herbal materials. PMID:21813258

  7. Design and implementation of room Agent based on MAS for intelligent building%基于MAS的智能建筑房间Agent的设计与实现

    王路; 谢秀颖; 张桂青; 汪明

    2011-01-01

    According to character of distributive building electrical equipment, multi-Agent system for buildings is designed to supply the more comfortable and more energy-efficient building, and the functions of room Agent ought is analyzed. On the basis of the wireless sensor networks and the Ethernet, the design proposal of the room Agent is provided. The problem of designing important part in the multi-Agent system is solved, the system runs more stable and the functions became stronger. Through testing multi-Agent system experimental platform, it is verified that the project is feasible and room Agent is reliable.%针对建筑用电设备的分布特征,为了给人类提供更加舒适建筑环境的同时也更加节能,设计了一种应用于智能建筑的多Agent系统,并分析了房间Agent(room Agent,RA)所应具备的功能,采用无线网络和以太网技术,提出了RA的硬件和软件设计方案,该方案不仅解决了多Agent系统设计的关键问题,而且使系统运行更加稳定,功能更加强大.通过测试搭建的多Agent系统实验平台,验证了方案的可实施性以及房间Agent的可靠性.

  8. An assessment of the three most commonly used sludge sedimentation indices; Valoracion de los tres indices de sedimentacion del fango mas utilizados

    Jorda, J. R.; Velasco, A.; Canas, P.

    2003-07-01

    Since Mohlman described, in 1934, the SVI as a quantitative measures of sludge settle ability, it has been the most commonly used method until today. This study compares three different volumetric indices, SVI, DSVI and SSVI, through experiments in seven reactors from WWTP during eight months. The analysis of the bibliography and the results advise against the use of the traditional SVI for several reasons and recommend the utilization of more modern indices as DSVI or SSVI. (Author) 11 refs.

  9. David A. BELL, The Cult of the Nation in France. Inventing Nationalism, 1680-1820, Cambridge (Mas.) et Londres, Harvard University Press, 2001, 304 p.

    Guilhaumou, Jacques

    2008-01-01

    David Bell, professeur d'histoire à la Johns Hopkins University, ne considère pas la nation comme une réalité dont l'essence aurait été définie par les Français du XVIIIe siècle. Dans la lignée de réflexions anglo-américaines (en particulier les travaux de Benedict Anderson, Ernest Gellner, Éric Hobsbawm et Anthony Smith), il l'appréhende plutôt comme un produit de la volonté politique, par le fait d'un artefact politique à construire dans un processus d'invention du nationalisme. Il donne ai...

  10. Inter- and intramolecular distance measurements by solid-state MAS NMR: Determination of gramicidin A channel dimer structure in hydrated phospholipid bilayers

    Fu Riqiang; Cotten, Myriam; Cross, Timothy A. [Center for Interdisciplinary Magnetic Resonance, National High Magnetic Field Laboratory (United States)

    2000-03-15

    Distance constraints are an important complement to orientational constraints. While a high-resolution monomer structure of the ion channel forming polypeptide, gramicidin A, has been solved with 120 orientational constraints, the precise geometry of the dimer interface has not been characterized. Here, using both {sup 13}C and {sup 15}N labeled gramicidin A samples in hydrated phospholipid bilayers, both inter- and intramolecular distances have been measured with a recently developed simultaneous frequency and amplitude modulation (SFAM) solid-state NMR scheme. Using this approach {sup 15}N-{sup 13}C{sub 1} residual dipolar couplings across a hydrogen bond as small as 20 {+-} 2 Hz have been characterized. While such distances are on the order of 4.2 {+-} 0.2 A, the spectroscopy is complicated by rapid global motion of the molecular structure about the bilayer normal and channel axis. Consequently, the nominal 40 Hz dipolar coupling is averaged depending on the orientation of the internuclear vector with respect to the motional axis. The intermolecular distance confirmed the previously described monomeric structure, while the intramolecular distance across the monomer-monomer interface defined this junction and confirmed the previous model of this interface.

  11. Örgütsel Cinsiyetlerin Örgütsel Davranışa Yansıması(Reflections Of Organizational Gender In Organizational Behavior)

    TEMEL, Ayşen; Mehmet YAKIN; Sema MİSCİ

    2006-01-01

    The concept of social gender is defined as all the values, roles and behaviors, which are related with women and men in society. The perception of social gender according to different societies affects the organizational behavior. Feminine and masculine characteristics of women and men in society according to their social roles are reflected in the job or in the way that they perform their job. Roles, behaviors, and values of the employees are determined according to the values, which are re...

  12. Genome Sequence of Salmonella enterica subsp. enterica Serovar Typhi Isolate PM016/13 from Untreated Well Water Associated with a Typhoid Outbreak in Pasir Mas, Kelantan, Malaysia.

    Muhamad Harish, Salwani; Sim, Kee-Shin; Najimudin, Nazalan; Aziah, Ismail

    2015-01-01

    Salmonella enterica subsp. enterica serovar Typhi is a human-restricted pathogen that causes typhoid fever. Even though it is a human-restricted pathogen, the bacterium is also isolated from environments such as groundwater and pond water. Here, we describe the genome sequence of the Salmonella enterica subsp. enterica serovar Typhi PM016/13 which was isolated from well water during a typhoid outbreak in Kelantan, Malaysia, in 2013. PMID:26564032

  13. Dynamics-based selective 2D {sup 1}H/{sup 1}H chemical shift correlation spectroscopy under ultrafast MAS conditions

    Zhang, Rongchun; Ramamoorthy, Ayyalusamy, E-mail: ramamoor@umich.edu [Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1055 (United States)

    2015-05-28

    Dynamics plays important roles in determining the physical, chemical, and functional properties of a variety of chemical and biological materials. However, a material (such as a polymer) generally has mobile and rigid regions in order to have high strength and toughness at the same time. Therefore, it is difficult to measure the role of mobile phase without being affected by the rigid components. Herein, we propose a highly sensitive solid-state NMR approach that utilizes a dipolar-coupling based filter (composed of 12 equally spaced 90° RF pulses) to selectively measure the correlation of {sup 1}H chemical shifts from the mobile regions of a material. It is interesting to find that the rotor-synchronized dipolar filter strength decreases with increasing inter-pulse delay between the 90° pulses, whereas the dipolar filter strength increases with increasing inter-pulse delay under static conditions. In this study, we also demonstrate the unique advantages of proton-detection under ultrafast magic-angle-spinning conditions to enhance the spectral resolution and sensitivity for studies on small molecules as well as multi-phase polymers. Our results further demonstrate the use of finite-pulse radio-frequency driven recoupling pulse sequence to efficiently recouple weak proton-proton dipolar couplings in the dynamic regions of a molecule and to facilitate the fast acquisition of {sup 1}H/{sup 1}H correlation spectrum compared to the traditional 2D NOESY (Nuclear Overhauser effect spectroscopy) experiment. We believe that the proposed approach is beneficial to study mobile components in multi-phase systems, such as block copolymers, polymer blends, nanocomposites, heterogeneous amyloid mixture of oligomers and fibers, and other materials.

  14. Analysis of local molecular motions of aromatic sidechains in proteins by 2D and 3D fast MAS NMR spectroscopy and quantum mechanical calculations

    Paluch, P.; Pawlak, T.; Jeziorna, A.; Trébosc, J.; Hou, G.; Vega, A. J.; Amoureux, J. P.; Dračínský, Martin; Polenova, T.; Potrzebowski, M. J.

    2015-01-01

    Roč. 17, č. 43 (2015), s. 28789-28801. ISSN 1463-9076 R&D Projects: GA ČR GA15-11223S Institutional support: RVO:61388963 Keywords : solid-state NMR * angle spinning NMR * NMR Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 4.493, year: 2014 http://pubs.rsc.org/en/content/articlepdf/2015/cp/c5cp04475h

  15. Application of Multivariete Analysis on High-Resolution Magic Angle Spinning (HR-MAS) 1H NMR Spectra of Rabbit Cornea

    Tessem, M. B.; Bathen, T. F.; Čejková, Jitka; Midelfart, A.

    Basel: Karger, 2004. s. 67. ISSN 0030-3747. [EVER 2004 (European Association for Vision and Eye Research). 24.09.2004-27.09.2004, Vilamoura] R&D Projects: GA ČR GA304/03/0419 Keywords : rabbit cornea Subject RIV: FF - HEENT, Dentistry

  16. 1 D Additive correction strategy for solving tow dimensional problem of heat and mas transfer in porous media with non-rectangular domain

    We propose a new procedure using 1 D additive correction strategy (AC), for resolution of tow dimensional problem of heat and mass transfer in field reactor of adsorption cooling machine. The reactor contains a porous medium constituted of activated carbon reacting by adsorption with ammonia. The present paper demonstrated how the new procedure of the (AC) propose here can be used, in the case of non-rectangular domain and strongly anisotropic coefficients, to improve the convergence rate of different iterative solvers currently used: Point Gauss-Seidel (GS), the line Gauss-Seidel (LGS), strongly implicit procedure (SIP) and the strongly implicit solver (SIS). Results shows that for different solvers, the performance of the additive correction strategy is efficiently improved by using the new procedure.(Author)

  17. Mas allá de la dependencia : hacia una teoría de la educación para los medios

    Buckingham, David

    2000-01-01

    En los últimos diez años, los profesores de medios de comunicación en el Reino Unido han empezado a desarrollar una teoría educativa sobre la Educación para los Medios, que se centra en el carácter específico de práctica pedagógica y comunicativa que tiene esta educación. Este artículo presenta tres elementos claves de esta teoría educativa: su énfasis en el conocimiento que tienen los jóvenes sobre los medios, su análisis de las funciones institucionales de la escolarización y su consideraci...

  18. Synthesis of ZSM-5 Zeolites with Defined Distribution of Al Atoms in the Framework and Multinuclear MAS NMR Analysis of the Control of Al Distribution

    Dědeček, Jiří; Balgová, V.; Pashková, Veronika; Klein, Petr; Wichterlová, Blanka

    2012-01-01

    Roč. 24, č. 16 (2012), s. 3231-3239. ISSN 0897-4756 R&D Projects: GA ČR GAP106/11/0624 Institutional support: RVO:61388955 Keywords : ZSM-5 zeolite * Al distribution * single Al atoms Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 8.238, year: 2012

  19. Detection of dynamic water molecules in a microcrystalline sample of the SH3 domain of {alpha}-spectrin by MAS solid-state NMR

    Chevelkov, Veniamin [Forschungsinstitut fuer Molekulare Pharmakologie (FMP) (Germany); Faelber, Katja [Max-Delbrueck-Centrum fuer Molekulare Medizin (Germany); Diehl, Anne [Forschungsinstitut fuer Molekulare Pharmakologie (FMP) (Germany); Heinemann, Udo [Max-Delbrueck-Centrum fuer Molekulare Medizin (Germany); Oschkinat, Hartmut; Reif, Bernd [Forschungsinstitut fuer Molekulare Pharmakologie (FMP) (Germany)], E-mail: reif@fmp-berlin.de

    2005-04-15

    Water molecules are a major determinant of protein stability and are important for understanding protein-protein interactions. We present two experiments which allow to measure first the effective T{sub 2} decay rate of individual amide proton, and second the magnetization build-up rates for a selective transfer from H{sub 2}O to H{sup N} using spin diffusion as a mixing element. The experiments are demonstrated for a uniformly {sup 2}H, {sup 15}N labeled sample of a microcrystalline SH3 domain in which exchangeable deuterons were back-substituted with protons. In order to evaluate the NMR experimental data, as X-ray structure of the protein was determined using the same crystallization protocol as for the solid-state NMR sample. The NMR experimental data are correlated with the dipolar couplings calculated from H{sub 2}O-H{sup N} distances which were extracted from the X-ray structure of the protein. We find that the H{sup N}T{sub 2} decay rates and H{sub 2}O-H{sup N} build-up rates are sensitive to distance and dynamics of the detected water molecules with respect to the protein. We show that qualitative information about localization and dynamics of internal water molecules can be obtained in the solid-state by interpretation of the spin dynamics of a reporter amide proton.

  20. Influence of terms sowing and methods cultivation on the mas inflorences and seeds of two form moldavian dragonhead (Dracocephalum moldavica L.

    Tadeusz Wolski

    2012-12-01

    Full Text Available Acquaintance of dependence among time - limit of sowing anfd with manner of tillage and with beginning of blooming and with recent mass of inflorescence and also of seeds has essential meaning both for economies in apiary, efficiencies of crop of seeds, which can be raw material that time - limit of sowing how and manner of tillage have influence on recent mass of inflorescence how and of seeds investigated od from dragonhead. One ascertained, that plants from fifth time - limit of sowing former unripe. One showed also, that both recent mass of inflorence how and of seeds independently from time - limit of sowing and of manner of tillage depends from circumstances climatically and former different in investigated years.