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Sample records for 3p 5q 17p

  1. Attention-Deficit/Hyperactivity Disorder in a Population Isolate: Linkage to Loci at 4q13.2, 5q33.3, 11q22, and 17p11

    Arcos-Burgos, Mauricio; Castellanos, F. Xavier; Pineda, David; Lopera, Francisco; David Palacio, Juan; Guillermo Palacio, Luis; Rapoport, Judith L.; Berg, Kate; Bailey-Wilson, Joan E.; Muenke, Maximilian

    2004-01-01

    Attention-deficit/hyperactivity disorder (ADHD [MIM 143465]) is the most common behavioral disorder of childhood. Twin, adoption, segregation, association, and linkage studies have confirmed that genetics plays a major role in conferring susceptibility to ADHD. We applied model-based and model-free linkage analyses, as well as the pedigree disequilibrium test, to the results of a genomewide scan of extended and multigenerational families with ADHD from a genetic isolate. In these families, ADHD is highly comorbid with conduct and oppositional defiant disorders, as well as with alcohol and tobacco dependence. We found evidence of linkage to markers at chromosomes 4q13.2, 5q33.3, 8q11.23, 11q22, and 17p11 in individual families. Fine mapping applied to these regions resulted in significant linkage in the combined families at chromosomes 4q13.2 (two-point allele-sharing LOD score from LODPAL = 4.44 at D4S3248), 5q33.3 (two-point allele-sharing LOD score from LODPAL = 8.22 at D5S490), 11q22 (two-point allele-sharing LOD score from LODPAL = 5.77 at D11S1998; multipoint nonparametric linkage [NPL] −log [P value] = 5.49 at ∼128 cM), and 17p11 (multipoint NPL −log [P value] >12 at ∼12 cM; multipoint maximum location score 2.48 [α = 0.10] at ∼12 cM; two-point allele-sharing LOD score from LODPAL = 3.73 at D17S1159). Additionally, suggestive linkage was found at chromosome 8q11.23 (combined two-point NPL−log [P value] >3.0 at D8S2332). Several of these regions are novel (4q13.2, 5q33.3, and 8q11.23), whereas others replicate already-published loci (11q22 and 17p11). The concordance between results from different analytical methods of linkage and the replication of data between two independent studies suggest that these loci truly harbor ADHD susceptibility genes. PMID:15497111

  2. RECENT ADVANCES IN THE 5Q- SYNDROME

    Andrea Pellagatti

    2015-05-01

    Full Text Available The 5q- syndrome is the most distinct of the myelodysplastic syndromes (MDS and patients with this disorder have a deletion of chromosome 5q [del(5q] as the sole karyotypic abnormality. Several genes mapping to the commonly deleted region of the 5q- syndrome have been implicated in disease pathogenesis in recent years. Haploinsufficiency of the ribosomal gene RPS14 has been shown to cause the erythroid defect in the 5q- syndrome. Loss of the microRNA genes miR-145 and miR-146a has been associated with the thrombocytosis observed in 5q- syndrome patients. Haploinsufficiency of CSNK1A1 leads to hematopoietic stem cell expansion in mice and may play a role in the initial clonal expansion in patients with 5q- syndrome. Moreover, a subset of patients harbor mutation of the remaining CSNK1A1 allele. Mouse models of the 5q- syndrome, which recapitulate the key features of the human disease, indicate that a p53-dependent mechanism underlies the pathophysiology of this disorder. Importantly, activation of p53 has been demonstrated in the human 5q- syndrome. Recurrent TP53 mutations have been associated with an increased risk of disease evolution and with decreased response to the drug lenalidomide in del(5q MDS patients. Potential new therapeutic agents for del(5q MDS include the translation enhancer L-leucine.

  3. Main: 1B5Q [RPSD[Archive

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  4. SMA type 2 unrelated to chromosome 5q13.

    Nevo, Y; Kramer, U; Legum, C; Shomrat, R; Fatal, A; Soffer, D; Harel, S; Shapira, Y

    1998-01-13

    We describe two brothers with clinical and histological findings of type 2 spinal muscular atrophy (SMA) associated with small head circumference (protein (NAIP) deletions were detected in these sibs, and they were discordant for the haplotypes determined by DNA markers flanking the 5q13 SMA locus. These findings support the presence of a distinct anterior horn disease unrelated to 5q13. This entity may have either autosomal recessive or X-linked inheritance. PMID:9450884

  5. Cytogenetic and molecular predictors of response in patients with myeloid malignancies without del[5q] treated with lenalidomide

    Sugimoto Yuka

    2012-03-01

    Full Text Available Abstract Background While lenalidomide (LEN shows high efficacy in myelodysplastic syndromes (MDS with del[5q], responses can be also seen in patients presenting without del[5q]. We hypothesized that improved detection of chromosomal abnormalities with new karyotyping tools may better predict response to LEN. Design and methods We have studied clinical, molecular and cytogenetic features of 42 patients with MDS, myeloproliferative neoplasms (MPN, MDS/MPN overlap syndromes and secondary acute myeloid leukemia (sAML without del[5q] by metaphase cytogenetics (MC who underwent therapy with LEN. Results Fluorescence in situ hybridization (FISH or single nucleotide polymorphism array (SNP-A-based karyotyping marginally increased the diagnostic yield over MC, detecting 2/42 (4.8% additional cases with del[5q], one of whom were responded to LEN. Responses were more often observed in patients with a normal karyotype by MC (60% vs abnormal MC; 17%, p = .08 and those with gain of chromosome 8 material by either of all 3 karyotyping methods (83% vs all other chromosomal abnormalities; 44% p = .11. However, 5 out of those 6 patients received combined LEN/AZA therapy and it may also suggest those with gain of chromosome 8 material respond well to AZA. The addition of FISH or SNP-A did not improve the predictive value of normal cytogenetics by MC. Mutational analysis of TET2, UTX, CBL, EZH2, ASXL1, TP53, RAS, IDH1/2, and DNMT-3A was performed on 21 of 41 patients, and revealed 13 mutations in 11 patients, but did not show any molecular markers of responsiveness to LEN. Conclusions Normal karyotype and gain of chromosome 8 material was predictive of response to LEN in non-del[5q] patients with myeloid malignancies.

  6. 3p deletion syndrome.

    Kaur, Anupam; Khetarpal, S

    2013-08-01

    3p deletion is a rare cytogenetic finding. Here we describe a 3 months old male with congenital malformations. His karyotype revealed 3p deletion 46,XY,del(3)(p25-pter). The child had flexion deformity of wrist and elbow which has never been reported before. PMID:24036645

  7. The duplication 17p13.3 phenotype

    Curry, Cynthia J; Rosenfeld, Jill A; Grant, Erica;

    2013-01-01

    duplications that include both the YWHAE and LIS1 genes. These patients had a relatively distinct facial phenotype and frequent structural brain abnormalities involving the corpus callosum, cerebellar vermis, and cranial base. Autism spectrum disorders were seen in a third of duplication probands, most......Chromosome 17p13.3 is a gene rich region that when deleted is associated with the well-known Miller-Dieker syndrome. A recently described duplication syndrome involving this region has been associated with intellectual impairment, autism and occasional brain MRI abnormalities. We report 34...

  8. EXTINCTION IN THE COMA OF COMET 17P/HOLMES

    On 2007 October 29, the outbursting comet 17P/Holmes passed within 0.''79 of a background star. We recorded the event using optical, narrowband photometry and detect a 3%-4% dip in stellar brightness bracketing the time of closest approach to the comet nucleus. The detected dimming implies an optical depth τ ≈ 0.04 at 1.''5 from the nucleus and an optical depth toward the nucleus center τn d = 0.006 ± 0.002 at α = 16° phase angle. Our measurements place the most stringent constraints on the extinction optical depth of any cometary coma.

  9. Transient Fragments in Outbursting Comet 17P/Holmes

    Stevenson, Rachel; Jewitt, David

    2010-01-01

    We present results from a wide-field imaging campaign at the Canada-France-Hawaii Telescope to study the spectacular outburst of comet 17P/Holmes in late 2007. Using image-processing techniques we probe inside the spherical dust coma and find sixteen fragments having both spatial distribution and kinematics consistent with isotropic ejection from the nucleus. Photometry of the fragments is inconsistent with scattering from monolithic, inert bodies. Instead, each detected fragment appears to be an active cometesimal producing its own dust coma. By scaling from the coma of the primary nucleus of 17P/Holmes, assumed to be 1.7 km in radius, we infer that the sixteen fragments have maximum effective radii between ~ 10 m and ~ 100 m on UT 2007 Nov. 6. The fragments subsequently fade at a common rate of ~ 0.2 mag/day, consistent with steady depletion of ices from these bodies in the heat of the Sun. Our characterization of the fragments supports the hypothesis that a large piece of material broke away from the nucle...

  10. Prenatal detection of 5q14.3 duplication including MEF2C and brain phenotype.

    Cesaretti, Claudia; Spaccini, Luigina; Righini, Andrea; Parazzini, Cecilia; Conte, Giorgio; Crosti, Francesca; Redaelli, Serena; Bulfamante, Gaetano; Avagliano, Laura; Rustico, Mariangela

    2016-05-01

    The 5q14.3 duplication is a rare condition comprising speech and developmental delay, microcephaly, and mild ventriculomegaly. The region 5q14.3 contains several genes but the predominant role for the onset of the neurodevelopmental phenotype has been attributed to MEF2C. We describe the prenatal identification of 5q14.3 duplication, including MEF2C, in a monochorionic twin pregnancy with corpus callosum anomalies, confirmed by autopsy. To the best of our knowledge, this cerebral finding has been observed for the first time in 5q14.3 duplication patients, possibly widening the neurological picture of this scarcely known syndrome. A pathogenetic role of MEF2C overexpression in brain development may be assumed, but further studies are needed. © 2016 Wiley Periodicals, Inc. PMID:26864752

  11. Lingering grains of truth around comet 17P/HOLMES

    Stevenson, R.; Bauer, J. M.; Mainzer, A. K.; Masiero, J. R. [Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Drive, MS 183-427, Pasadena, CA 91109 (United States); Kramer, E. A. [Department of Physics, University of Central Florida, 4000 Central Florida Blvd, Orlando, FL 32816 (United States); Grav, T., E-mail: Rachel.A.Stevenson@jpl.nasa.gov [Planetary Science Institute, 1700 East Fort Lowell, Suite 106, Tucson, AZ 85719-2395 (United States)

    2014-06-01

    Comet 17P/Holmes underwent a massive outburst in 2007 October, brightening by a factor of almost a million in under 48 hr. We used infrared images taken by the Wide-Field Infrared Survey Explorer mission to characterize the comet as it appeared at a heliocentric distance of 5.1 AU almost 3 yr after the outburst. The comet appeared to be active with a coma and dust trail along the orbital plane. We constrained the diameter, albedo, and beaming parameter of the nucleus to 4.135 ± 0.610 km, 0.03 ± 0.01, and 1.03 ± 0.21, respectively. The properties of the nucleus are consistent with those of other Jupiter family comets. The best-fit temperature of the coma was 134 ± 11 K, slightly higher than the blackbody temperature at that heliocentric distance. Using Finson-Probstein modeling, we found that the morphology of the trail was consistent with ejection during the 2007 outburst and was made up of dust grains between 250 μm and a few cm in radius. The trail mass was ∼1.2-5.3 × 10{sup 10} kg.

  12. Lingering grains of truth around comet 17P/Holmes

    Stevenson, R; Kramer, E A; Grav, T; Mainzer, A K; Masiero, J R

    2014-01-01

    Comet 17P/Holmes underwent a massive outburst in 2007 Oct., brightening by a factor of almost a million in under 48 hours. We used infrared images taken by the Wide-Field Survey Explorer mission to characterize the comet as it appeared at a heliocentric distance of 5.1 AU almost 3 years after the outburst. The comet appeared to be active with a coma and dust trail along the orbital plane. We constrained the diameter, albedo, and beaming parameter of the nucleus to 4.135 $\\pm$ 0.610 km, 0.03 $\\pm$ 0.01 and 1.03 $\\pm$ 0.21, respectively. The properties of the nucleus are consistent with those of other Jupiter Family comets. The best-fit temperature of the coma was 134 $\\pm$ 11 K, slightly higher than the blackbody temperature at that heliocentric distance. Using Finson-Probstein modeling we found that the morphology of the trail was consistent with ejection during the 2007 outburst and was made up of dust grains between 250 $\\mu$m and a few cm in radius. The trail mass was $\\sim$ 1.2 - 5.3 $\\times$ 10$^{10}$ kg...

  13. Water outburst activity in Comet 17P/Holmes

    de Almeida, Amaury A.; Boice, Daniel C.; Picazzio, Enos; Huebner, Walter F.

    2016-08-01

    Cometary outbursts are sporadic events whose mechanisms are not well known where the activity and consequently the brightness can increase hundreds of thousands of times within a few hours to several days. This indicates a dramatic departure from thermal equilibrium between the comet and interplanetary space and is usually documented by "light curves". In a typical cometary outburst, the brightness can increase by 2-5 magnitudes (Whitney, 1955; Gronkowski and Wesolowski, 2015). In only 42 h, Comet 17P/Holmes was reported to brighten from a magnitude of about 17 to about 2.4 at the height of the burst, representing the largest known outburst by a comet. We present the H2O production rate of Holmes for the megaburst occurring between 23 and 24 October 2007. For this, we selected more than 1900 photometric observations from the International Comet Quarterly Archive of Photometric Data (Green, 2007) and use the Semi-Empirical Method of Visual Magnitudes (SEMVM; de Almeida et al., 2007). We clearly show that the comet achieved an average water production rate of 5 × 1029 molecules s-1, corresponding to a water gas loss rate of 14,960 kg s-1, in very good agreement with Schleicher (2009) who derived the water production rate using OH measurements on 1 Nov 2007 (about 8 days after the outburst). We discuss possible physical processes that might cause cometary outbursts and propose a new qualitative mechanism, the Pressurized Obstructed Pore (POP) model. The key feature of POP is the recrystallization of water in the surface regolith as it cools, plugging pores and blocking the release of subsurface gas flow. As the interior gas pressure increases, an outburst is eventually triggered. POP is consistent with current observations and can be tested in the future with observations (e.g., Rosetta in situ measurements) and detailed simulations.

  14. A family with an inverted tandem duplication 5q22.1q23.2.

    Schmidt, T; Bartels, I; Liehr, T; Burfeind, P; Zoll, B; Shoukier, M

    2013-01-01

    Here, we report a 3-year-old boy with short stature, developmental delay and mild facial dysmorphic signs. Karyotype analysis and array-CGH revealed a pure duplication 5q22.1q23.2 with a length of 14.25 Mb. As demonstrated by multicolor-fluorescence in situ hybridization, the duplicated segment was orientated in an inverted tandem manner. One of the 2 older half-brothers of the index patient was intellectually disabled and showed short stature as well. The mother of the siblings was only 149 cm in height. The affected half-brother as well as the mother of the siblings were tested positive for the same duplication. Duplications of the long arm of chromosome 5 are rare. There are 16 reported cases of different 5q segments with a pure duplication and no additional chromosomal imbalance. In order to refine the 5q-duplication phenotype, reported cases were recently classified in 3 groups on the basis of clinical findings and the involved chromosome segments. However, our case does not fit in any of these groups but is placed in the interjacent chromosomal area between 2 of these groups. Overall, this is the second reported family with a duplication of 5q22.1q23.2 and both families share phenotypic features like short stature, facial dysmorphic signs and speech delay. The reported family provides further information for delineating phenotype-genotype correlations of pure duplications of the 5q region. PMID:23051634

  15. Cytogenetic and molecular cytogenetic findings in 43 aneurysmal bone cysts: aberrations of 17p mapped to 17p13.2 by fluorescence in situ hybridization.

    Althof, Pamela A; Ohmori, Kazuo; Zhou, Ming; Bailey, Jacqueline M; Bridge, R Stuart; Nelson, Marilu; Neff, James R; Bridge, Julia A

    2004-05-01

    Aneurysmal bone cyst is a benign, cystic lesion of bone composed of blood-filled spaces separated by fibrous septa. Relatively few cases of aneurysmal bone cyst have been cytogenetically characterized, yet abnormalities of the short arm of chromosome 17 appear to be recurrent. In this study, conventional cytogenetic analysis of 43 aneurysmal bone cyst specimens from 38 patients over a 12-year period revealed clonal chromosomal abnormalities in 12 specimens. Karyotypic anomalies of 17p, including a complex translocation and inversion, were identified in eight of these 12 specimens. In an effort to further define the aberrant 17p breakpoint, fluorescence in situ hybridization (FISH) analyses were performed using a series of probe combinations spanning a 5.1 Mb region between the TP53 (17p13.1) and Miller-Dieker lissencephaly syndrome (17p13.3) gene loci. These studies revealed the critical breakpoint locus at 17p13.2, flanked proximally by an RP11-46I8, RP11-333E1, and RP11-457I18 bacterial artificial chromosome (BAC) probe cocktail and distally by an RP11-198F11 and RP11-115H24 BAC and RP5-1050D4 P1 artificial chromosome (PAC) probe cocktail. Overall, abnormalities of the 17p13.2 locus were identified by metaphase and/or interphase cell FISH analysis in 22 of 35 (63%) aneurysmal bone cyst specimens examined including 26 karyotypically normal specimens. These cytogenetic and molecular cytogenetic findings expand our knowledge of chromosomal alterations in aneurysmal bone cyst, further localize the critically involved 17p breakpoint, and provide an alternative approach (ie FISH) for detecting 17p abnormalities in nondividing cells of aneurysmal bone cysts. The latter could potentially be utilized as an adjunct in diagnostically challenging cases. PMID:15044915

  16. Attention-Deficit/Hyperactivity Disorder in a Population Isolate: Linkage to Loci at 4q13.2, 5q33.3, 11q22, and 17p11

    Arcos-Burgos, Mauricio; Castellanos, F. Xavier; Pineda, David; Lopera, Francisco; David Palacio, Juan; Guillermo Palacio, Luis; Rapoport, Judith L.; BERG, KATE; Bailey-Wilson, Joan E.; Muenke, Maximilian

    2004-01-01

    Attention-deficit/hyperactivity disorder (ADHD [MIM 143465]) is the most common behavioral disorder of childhood. Twin, adoption, segregation, association, and linkage studies have confirmed that genetics plays a major role in conferring susceptibility to ADHD. We applied model-based and model-free linkage analyses, as well as the pedigree disequilibrium test, to the results of a genomewide scan of extended and multigenerational families with ADHD from a genetic isolate. In these families, AD...

  17. Duplication of 5q21 in a mildly retarded male and his non-retarded mother

    Stallard, R.; Zurcher, V.; Schwartz, S. [Case Western Reserve Univ., Cleveland, OH (United States)

    1994-09-01

    Euchromatic autosomal additions to chromosomal complements are typically associated with global effects including mental retardation (MR) and dysmorphism. We report a familial duplication that does not appear to cause consistent, significant effects. A hyperactive male with mild MR was referred for fra(X) testing at 8 yrs. His karyotype was fra(X) negative and normal except for an addition in one 5q. The abnormal 5 was also in the maternal karyotype, but all other parental chromosomes were normal. The addition (=8.5% the length of a 5) was interpreted as a duplication of band 5q21. FISH with Coatasome 5 (Oncor) showed the addition was from 5. The proband`s karyotype was designated 46,XY,dup(5)(q15q22.1)mat; his mother`s, 46,XX,dup(5)(q15q22.1). Single copy probes are being used to test the cytogenetic interpretation. At 39 yrs, the non-retarded, somewhat inattentive mother, who has a high school diploma and subsequent secretarial courses, cares for the proband and his chromosomally normal, but learning disabled sister at home. The family situation is chaotic with reported paternal psychiatric illness and abuse of the proband and his sister. The mother`s father is dead, but her four younger siblings and mother are reportedly normal. Their chromosomes have not been available. The proband was born at 40 weeks following an uneventful pregnancy, with length and weight at the 5-10th centiles. He walked and talked at about one year. At 9 yrs, his ht/wt ratio was 10th centile. Foot length as <3rd centile; soft masses were present on the anterior ankles. He was otherwise physically normal. His estimated I.Q. was 75 and he was severely hyperactive despite Ritalin. This is the first report of a familial duplication in 5q; no identical, isolated case is known. Although additional family members need evaluation, the presence of the dup(5q) in the non-retarded mother suggests that it may not be associated with the proband`s MR.

  18. 17 CFR 170.10 - Proficiency examinations (sections 4p and 17(p) of the Act).

    2010-04-01

    ... 17 Commodity and Securities Exchanges 1 2010-04-01 2010-04-01 false Proficiency examinations (sections 4p and 17(p) of the Act). 170.10 Section 170.10 Commodity and Securities Exchanges COMMODITY... examinations (sections 4p and 17(p) of the Act). A futures association may prescribe different...

  19. LOSS OF HETEROZYGOSITY ON CHROMOSOME 17p13.3 IN OVARIAN CANCER AND CERVICAL CANCER

    Zhang Guoling; Yang Huijian; Xu Kaili; Zhou Jin; Qin Ruidi; Lu Minghua

    1998-01-01

    Objective:To identify the loss of heterozygosity (LOH) on chromosome 17p13.3 in ovarian cancer and cervical cancer. Methods: The frequency of LOH on chromosome 17p13.3 in DNA samples from 24 ovarian cancers, 9 cervical cancers, and 13 non-malignant gynecological diseases were determined respectively, using Southern blot method with probe PYNZ.22. Results:LOH on 17p13.3 was found in 12 of 24 (50.0%) ovarian cancers (including a borderline mucinous cystadenoma), 4of 9 (44.4%) cervical carcinomas, and 1 of 13 (7.7%) nonmalignant gynecological diseases, which was cervical intraepithelial neoplasm HI (CIN Ⅲ) (P<0.01).Conclusion: These results show that LOH on 17p13.3 is associated with ovarian cancer and cervical cancer,suggesting that detection of LOH on 17p13.3 may be helpful to understand the molecular pathogenesis of ovarian cancer and cervical cancer.

  20. An unusual combination of trisomy 21 and partial trisomy 5q.

    Kim, C J; Chi, J. G.; K. H. Lee; Lee, C. K.; Yoo, M. S.; Paik, Y K

    1992-01-01

    The authors describe a male newborn with multiple congenital anomalies; craniofacial dysmorphism, bilateral cleft palate and lip, ambiguous external genitalia with absence of phallus, ventricular septal defect, agenesis of olfactory bulbs, and presence of small round cells simulating migration defect in the cerebellar white matter. Cytogenetic study demonstrated a chromosomal constitution of 47,XY, +21, +5q. Its pathological significance compared with Down's syndrome and hitherto reported par...

  1. Discovery of Genetic Variation on Chromosome 5q22 Associated with Mortality in Heart Failure

    Smith, J. Gustav; Felix, Janine F.; Morrison, Alanna C.; Kalogeropoulos, Andreas; Trompet, Stella; Wilk, Jemma B.; Gidlöf, Olof; Wang, Xinchen; Morley, Michael; Mendelson, Michael; Joehanes, Roby; Ligthart, Symen; Shan, Xiaoyin; Bis, Joshua C.; Wang, Ying A.

    2016-01-01

    Failure of the human heart to maintain sufficient output of blood for the demands of the body, heart failure, is a common condition with high mortality even with modern therapeutic alternatives. To identify molecular determinants of mortality in patients with new-onset heart failure, we performed a meta-analysis of genome-wide association studies and follow-up genotyping in independent populations. We identified and replicated an association for a genetic variant on chromosome 5q22 with 36% i...

  2. Genetic and radiation hybrid mapping of the hyperekplexia region on chromosome 5q.

    Ryan, S. G.; Dixon, M J; Nigro, M A; Kelts, K A; Markand, O N; Terry, J C; Shiang, R; Wasmuth, J J; O'Connell, P

    1992-01-01

    Hyperekplexia, or startle disease (STHE), is an autosomal dominant neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to sudden, unexpected acoustic or tactile stimuli. STHE responds dramatically to the benzodiazepine drug clonazepam, which acts at gamma-aminobutyric acid type A (GABA-A) receptors. The STHE locus (STHE) was recently assigned to chromosome 5q, on the basis of tight...

  3. Bilateral radial agenesis with absent thumbs, complex heart defect, short stature, and facial dysmorphism in a patient with pure distal microduplication of 5q35.2-5q35.3

    Jamsheer Aleksander

    2013-01-01

    Full Text Available Abstract Background A partial duplication of the distal long arm of chromosome 5 (5q35-- > qter is known to be associated with a distinct phenotype referred to as Hunter-McAlpine syndrome. Clinical spectrum of this disorder mainly consists of mental retardation, microcephaly, short stature, skeletal anomalies, and craniofacial dysmorphism featuring flat facies, micrognathia, large, low-set dysplastic ears, hypertelorism, almond-shaped, down-slanted palpebral fissures, epicanthal folds, small nose, long philtrum, small mouth, and thin upper lip. Less frequent remarkable findings include craniosynostosis, heart defect, hypoplastic phalanges, preaxial polydactyly, hypospadias, cryptorchidism, and inguinal hernia. In most patients with a partial duplication of 5q the aberration occurred due to an inherited unbalanced translocation, therefore the phenotype was not reflective of pure trisomy 5q. Case presentation We report on a 9.5-year-old boy with some feature of Hunter-McAlpine syndrome including short stature, complex heart defect (dextrocardia, dextroversion, PFO, bilateral cryptorchidism, hypothyroidism, and craniofacial dysmorphism. Additionally, bilateral radial agenesis with complete absence of Ist digital rays, ulnar hypoplasia with bowing, choroidal and retinal coloboma, abnormal biliary vesicle were identified, which have never been noted in 5q trisomy patients. Karyotype analysis, sequencing and MLPA for TBX5 and SALL4 genes were unremarkable. Array comparative genomic hybridization detected a duplication on 5q35.2-5q35.3, resulting from a de novo chromosomal rearrangement. Our proband carried the smallest of all previously reported pure distal 5q trisomies encompassing terminal 5.4-5.6 Mb and presented with the most severe limb malformation attributed to the increased number of distal 5q copies. Conclusions We postulate that a terminal distal trisomy of 5q35.2-5q35.3, which maps 1.1 Mb telomeric to the MSX2 gene is causative for both

  4. Genetic and radiation hybrid mapping of the hyperekplexia region on chromosome 5q

    Ryan, S.G.; O' Connell, P. (Univ. of Texas Health Science Center, San Antonio (United States)); Dixon, M.J. (Univ. of Manchester (United Kingdom)); Nigro, M.A. (Wayne State Univ., Detroit, MI (United States)); Kelts, K.A. (Black Hills Neurology, Rapid City, SD (United States)); Markand, O.N. (Indiana Univ., Indianopolis (United States)); Shiang, R.; Wasmuth, J.J. (Univ. of California, Irvine (United States)); Terry, J.C.

    1992-12-01

    Hyperekplexia, or startle disease (STHE), is an autosomal dominant neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to sudden, unexpected acoustic or tactile stimuli. STHE responds dramatically to the benzodiazepine drug clonazepam, which acts at gamma-aminobutyric acid type A (GABA-A) receptors. The STHE locus (STHE) was recently assigned to chromosome 5q, on the basis of tight linkage to the colony-stimulating factor 1-receptor (CSF1-R) locus in a single large family. The authors performed linkage analysis in the original and three additional STHE pedigrees with eight chromosome 5q microsatellite markers and placed several of the most closely linked markers on an existing radiation hybrid (RH) map of the region. The results provide strong evidence for genetic locus homogeneity and assign STHE to a 5.9-cM interval defined by CSF1-R and D5S379, which are separated by an RH map distance of 74 centirays (roughly 2.2-3.7 Mb). Two polymorphic markers (D5S119 and D5S209) lie within this region, but they could not be ordered with respect to STHE. RH mapping eliminated the candidate genes GABRA1 and GABRG2, which encode GABA-A receptor components, by showing that they are telomeric to the target region. 45 refs., 4 figs., 4 tabs.

  5. Chromosome 17p deletion in human medulloblastoma: a missing checkpoint in the Hedgehog pathway.

    De Smaele, Enrico; Di Marcotullio, Lucia; Ferretti, Elisabetta; Screpanti, Isabella; Alesse, Edoardo; Gulino, Alberto

    2004-10-01

    Although deregulation of Hedgehog signalling is considered to play a crucial oncogenic role and commonly occurrs in medulloblastoma, genetic lesions in components of this pathway are observed in a minority of cases. The recent identification of a novel putative tumor suppressor (REN(KCTD11)) on chromosome 17p13.2, a region most frequently lost in human medulloblastoma, highlights the role of allelic deletion of the gene in this brain malignancy, leading to the loss of growth inhibitory activity via suppression of Gli-dependent activation of Hedgehog target genes. The presence on 17p13 of another tumor suppressor gene (p53) whose inactivation cooperates with Hedgehog pathway for medulloblastoma formation, suggests that 17p deletion unveils haploinsufficiency conditions leading to abrogation of either direct and indirect checkpoints of Hedgehog signalling in cancer. PMID:15467454

  6. Variation in conserved non-coding sequences on chromosome 5q andsusceptibility to asthma and atopy

    Donfack, Joseph; Schneider, Daniel H.; Tan, Zheng; Kurz,Thorsten; Dubchak, Inna; Frazer, Kelly A.; Ober, Carole

    2005-09-10

    Background: Evolutionarily conserved sequences likely havebiological function. Methods: To determine whether variation in conservedsequences in non-coding DNA contributes to risk for human disease, westudied six conserved non-coding elements in the Th2 cytokine cluster onhuman chromosome 5q31 in a large Hutterite pedigree and in samples ofoutbred European American and African American asthma cases and controls.Results: Among six conserved non-coding elements (>100 bp,>70percent identity; human-mouse comparison), we identified one singlenucleotide polymorphism (SNP) in each of two conserved elements and sixSNPs in the flanking regions of three conserved elements. We genotypedour samples for four of these SNPs and an additional three SNPs each inthe IL13 and IL4 genes. While there was only modest evidence forassociation with single SNPs in the Hutterite and European Americansamples (P<0.05), there were highly significant associations inEuropean Americans between asthma and haplotypes comprised of SNPs in theIL4 gene (P<0.001), including a SNP in a conserved non-codingelement. Furthermore, variation in the IL13 gene was strongly associatedwith total IgE (P = 0.00022) and allergic sensitization to mold allergens(P = 0.00076) in the Hutterites, and more modestly associated withsensitization to molds in the European Americans and African Americans (P<0.01). Conclusion: These results indicate that there is overalllittle variation in the conserved non-coding elements on 5q31, butvariation in IL4 and IL13, including possibly one SNP in a conservedelement, influence asthma and atopic phenotypes in diversepopulations.

  7. Functional effects of 17alpha-hydroxyprogesterone caproate (17P on human myometrial contractility in vitro

    Morrison John J

    2004-12-01

    Full Text Available Abstract Background 17alpha-hydroxyprogesterone caproate (17P administration reportedly improves outcome for women with a previous spontaneous preterm delivery. This study, using in vitro strips of human uterine smooth muscle, aimed to investigate the direct non-genomic effects of 17P on spontaneous and induced contractions in tissues obtained during pregnancy, and in the non-pregnant state. Methods Biopsies of human myometrium were obtained at elective cesarean section, and from hysterectomy specimens, and dissected strips suspended for isometric recordings. The effects of 17P (1 nmol/L -10 micro mol/L on spontaneous and agonist-induced (oxytocin 0.5 nmol/L for pregnant, phenylephrine 10 μmol/L for non-pregnant contractions were measured. Integrals of contractile activity, including the mean maximal inhibition values (MMI observed at the maximal concentration, were compared with those from simultaneously run control strips. Results There was no significant direct effect exerted by 17P on pregnant or non-pregnant human myometrial contractility. The MMI ± SEM for spontaneous contractions in pregnant myometrium was 4.9% ± 7.2 (n = 6; P = 0.309 and for oxytocin-induced contractions was 2.2% ± 1.3 (n = 6; P = 0.128. For non-pregnant myometrium, the MMI ± SEM for spontaneous contractions was 8.8% ± 11.0 (n = 6; P = 0.121 and for phenylephrine induced contractions was -7.9% ± 6.5 (n = 6; P = 0.966. Conclusions The putative benefits of 17P for preterm labor prevention are not achieved, even partially, by a direct utero-relaxant effect. These findings outline the possibility that genomic effects of 17P, achieved over long periods of administration, are required for its reported therapeutic benefits.

  8. Discovery of Genetic Variation on Chromosome 5q22 Associated with Mortality in Heart Failure

    Smith, J. Gustav; Felix, Janine F.; Morrison, Alanna C.; Trompet, Stella; Wilk, Jemma B.; Gidlöf, Olof; Morley, Michael; Joehanes, Roby; Ligthart, Symen; Shan, Xiaoyin; Bis, Joshua C.; Sjögren, Marketa; Ngwa, Julius; Stott, David J.; Aguilar, David; Rice, Kenneth M.; Sesso, Howard D.; Demissie, Serkalem; Buckley, Brendan M.; Taylor, Kent D.; Ford, Ian; Yao, Chen; Liu, Chunyu; Sotoodehnia, Nona; van der Harst, Pim; Stricker, Bruno H. Ch.; Kritchevsky, Stephen B.; Liu, Yongmei; Gaziano, J. Michael; Hofman, Albert; Moravec, Christine S.; Uitterlinden, André G.; Kellis, Manolis; van Meurs, Joyce B.; Margulies, Kenneth B.; Dehghan, Abbas; Levy, Daniel; Olde, Björn; Psaty, Bruce M.; Cupples, L. Adrienne; Jukema, J. Wouter; Djousse, Luc; Franco, Oscar H.; Boerwinkle, Eric; Boyer, Laurie A.; Newton-Cheh, Christopher; Butler, Javed; Vasan, Ramachandran S.; Cappola, Thomas P.; Smith, Nicholas L.

    2016-01-01

    Failure of the human heart to maintain sufficient output of blood for the demands of the body, heart failure, is a common condition with high mortality even with modern therapeutic alternatives. To identify molecular determinants of mortality in patients with new-onset heart failure, we performed a meta-analysis of genome-wide association studies and follow-up genotyping in independent populations. We identified and replicated an association for a genetic variant on chromosome 5q22 with 36% increased risk of death in subjects with heart failure (rs9885413, P = 2.7x10-9). We provide evidence from reporter gene assays, computational predictions and epigenomic marks that this polymorphism increases activity of an enhancer region active in multiple human tissues. The polymorphism was further reproducibly associated with a DNA methylation signature in whole blood (P = 4.5x10-40) that also associated with allergic sensitization and expression in blood of the cytokine TSLP (P = 1.1x10-4). Knockdown of the transcription factor predicted to bind the enhancer region (NHLH1) in a human cell line (HEK293) expressing NHLH1 resulted in lower TSLP expression. In addition, we observed evidence of recent positive selection acting on the risk allele in populations of African descent. Our findings provide novel genetic leads to factors that influence mortality in patients with heart failure. PMID:27149122

  9. Energy and Auger Widths of Triply Excited 3p3p3p 2po State of Lithium

    苟秉聪; 俞开智

    2003-01-01

    The high-lying triply excited 3p3p3p 2po state of the double hollow lithium atom is studied by using the saddlepoint complex-rotation method. The energy and Auger widths of this resonance are calculated. The relativistic corrections and mass polarization are included. The total Auger width is obtained by coupling the important open channels and summing over the other channels. The oscillator strength is also calculated. The results are compared with other theoretical and experimental data in the literature.

  10. p53 protein expression independently predicts outcome in patients with lower-risk myelodysplastic syndromes with del(5q)

    Saft, L.; Karimi, M.; Ghaderi, M.; Matolcsy, A.; Mufti, G.J.; Kulasekararaj, A.; Gohring, G.; Giagounidis, A.; Selleslag, D.; Muus, P.; Sanz, G.; Mittelman, M.; Bowen, D.; Porwit, A.; Fu, T.; Backstrom, J.; Fenaux, P.; MacBeth, K.J.; Hellstrom-Lindberg, E.

    2014-01-01

    Del(5q) myelodysplastic syndromes defined by the International Prognostic Scoring System as low- or intermediate-1-risk (lower-risk) are considered to have an indolent course; however, recent data have identified a subgroup of these patients with more aggressive disease and poorer outcomes. Using de

  11. Cancer of the ampulla of Vater: chromosome 17p allelic loss is associated with poor prognosis

    Scarpa, A.; Di, P; Talamini, G; Falconi, M; Lemoine, N; Iacono, C.; Achille, A.; Baron, A; Zamboni, G

    2000-01-01

    BACKGROUND—Cancer of the ampulla of Vater kills 60% of affected patients. Local spread of the tumour (T stage) is the only reliable prognostic factor. Nevertheless, any cancer stage includes long term survivors and patients dying from the disease. The molecular anomalies involved in this process have the potential to serve as additional prognostic markers.
AIM—To evaluate if allelic losses (LOH) of chromosomes 17p and 18q may be of prognostic value in multivariate survival analysis.
METHODS—W...

  12. Association analysis of GABA receptor subunit genes on 5q33 with heroin dependence in a Chinese male population.

    Loh, E W; Tang, N L S; Lee, D T S; Liu, S I; Stadlin, Alfreda

    2007-06-01

    GABAA receptor subunit genes clustered on 5q33 play a role in the development of alcoholism and methamphetamine use disorder without psychosis. The present study explored the possible contribution of the same subunit genes to the development of heroin dependence. Single nucleotide polymorphisms (SNPs) of the GABAA receptor subunits GABRB2, GABRA6, GABRA1, and GABRG2 were examined in 178 male Han Chinese heroin-dependent and 170 male control subjects. A significant difference in allele frequency for the SNP rs211014 in the GABAAgamma2 receptor subunit gene between cases and controls was identified (P = 0.015). A possible mechanism for the involvement of the GABA receptor subunit genes on 5q33 in the development of heroin dependence is discussed. PMID:17440936

  13. Two cases of 5q deletions in patients with familial adenomatous polyposis: possible link with Caroli's disease.

    Hodgson, S V; Coonar, A S; P. J. Hanson; Cottrell, S; Scriven, P N; Jones, T; Hawley, P R; Wilkinson, M L

    1993-01-01

    Two cases are reported of patients with deletions of chromosome 5q. Both have familial adenomatous polyposis (FAP) and mild mental retardation. In both, macroscopic polyposis was confined to the proximal colon in adult life (in their thirties) although microscopic adenomatosis was shown in the more distal colon with occasional single polyps. Both subjects had dermoid cysts, and congenital hypertrophy of the retinal pigment epithelium (CHRPE) was seen in case 2. Case 1 has gastroduodenal polyp...

  14. Allelic imbalance and fine mapping of the 17p13.3 subregion in sporadic breast carcinomas

    Hoff, C; Mollenhauer, J; Waldau, B;

    2001-01-01

    region is suspected to harbor another tumor suppressor gene. In order to get more information concerning the pattern of AIs in 17p13.3, we performed analysis of AI of 49 breast carcinomas at 6 polymorphic loci in 17p13.3. Eighty-six percent of the tumors present AI at least at one marker in 17p13......Chromosome arm 17p is frequently altered in a variety of human cancers, especially in breast cancer, and allelic imbalances (AIs) in the region 17p13.1 do not always coincide with mutations in the TP53 gene. A second interval that frequently shows AIs at 17p is the chromosomal band 17p13.3. This.......3. Among all loci tested, the highest percentage of Al was observed at loci D17S5 (77%) and D17S1528 (72%). According to these results, a minimal region of deletion could be determined between the markers D17S28 and D17S5. Fine mapping of this region revealed that the size of the deleted region is about...

  15. Thermoelastic properties of Zn3P2

    Gerward, Leif; Olsen, J. Staun; Waśkowska, A.

    2011-01-01

    The bulk modulus and thermal expansion of Zn3P2 has been investigated at pressures up to 21GPa and temperatures down to 100K. The experimental zero-pressure bulk modulus is 80.7 ± 1.8GPa, in accordance with the bulk modulus scaling and lattice properties of the related compound Cd3P2. A tetragonal...... to orthorhombic phase transformation occurs above 11GPa with a relative volume change of-7.1%. Values for the thermal expansion coefficient are reported at 293, 200 and 100K....

  16. Fine-scale mapping of the 5q11.2 breast cancer locus reveals at least three independent risk variants regulating MAP3K1

    D.M. Glubb (Dylan); M. Maranian (Melanie); K. Michailidou (Kyriaki); K.A. Pooley (Karen); K.B. Meyer (Kerstin); S. Kar (Siddhartha); A.F.C. Carlebur; M. O'Reilly (Marian); J.A. Betts (Joshua); K.M. Hillman (Kristine); S. Kaufmann (Susanne); J. Beesley (Jonathan); S. Canisius (Sander); J.L. Hopper (John); M.C. Southey (Melissa); H. Tsimiklis (Helen); C. Apicella (Carmel); M.K. Schmidt (Marjanka); A. Broeks (Annegien); F.B.L. Hogervorst (Frans); C.E. van der Schoot (Ellen); K.R. Muir (K.); A. Lophatananon (Artitaya); S. Stewart-Brown (Sarah); P. Siriwanarangsan (Pornthep); P.A. Fasching (Peter); M. Ruebner (Matthias); A.B. Ekici (Arif); M.W. Beckmann (Matthias W.); J. Peto (Julian); I. dos Santos Silva (Isabel); O. Fletcher (Olivia); N. Johnson (Nichola); P.D.P. Pharoah (Paul D.P.); M.K. Bolla (Manjeet); Q. Wang (Qing); J. Dennis (Joe); E.J. Sawyer (Elinor); I.P. Tomlinson (Ian); M. Kerin (Michael); N. Miller (Nicola); B. Burwinkel (Barbara); F. Marme (Federick); R. Yang (Rongxi); H. Surowy (Harald); P. Guénel (Pascal); T. Truong (Thérèse); F. Menegaux (Florence); M. Sanchez (Marie); S.E. Bojesen (Stig); B.G. Nordestgaard (Børge); S.F. Nielsen (Sune); H. Flyger (Henrik); A. González-Neira (Anna); J. Benítez (Javier); M.P. Zamora (Pilar); J.I. Arias Pérez (José Ignacio); H. Anton-Culver (Hoda); S.L. Neuhausen (Susan); H. Brenner (Hermann); A.K. Dieffenbach (Aida Karina); V. Arndt (Volker); C. Stegmaier (Christa); A. Meindl (Alfons); R.K. Schmutzler (Rita); H. Brauch (Hiltrud); Y.-D. Ko (Yon-Dschun); T. Brüning (Thomas); H. Nevanlinna (Heli); T.A. Muranen (Taru); K. Aittomäki (Kristiina); C. Blomqvist (Carl); K. Matsuo (Keitaro); H. Ito (Hidemi); H. Iwata (Hisato); H. Tanaka (Hideo); T. Dörk (Thilo); N.V. Bogdanova (Natalia); S. Helbig (Sonja); A. Lindblom (Annika); S. Margolin (Sara); A. Mannermaa (Arto); V. Kataja (Vesa); V-M. Kosma (Veli-Matti); J.M. Hartikainen (J.); A.H. Wu (Anna H.); C.-C. Tseng (Chiu-Chen); D. Van Den Berg (David); D.O. Stram (Daniel O.); D. Lambrechts (Diether); H. Zhao (Hui); C. Weltens (Caroline); E. van Limbergen (Erik); J. Chang-Claude (Jenny); D. Flesch-Janys (Dieter); A. Rudolph (Anja); P. Seibold (Petra); P. Radice (Paolo); P. Peterlongo (Paolo); M. Barile (Monica); F. Capra (Fabio); F.J. Couch (Fergus); J.E. Olson (Janet); B. Hallberg (Boubou); C. Vachon (Celine); G.G. Giles (Graham); R.L. Milne (Roger); C.A. McLean (Catriona Ann); C.A. Haiman (Christopher); B.E. Henderson (Brian); F.R. Schumacher (Fredrick); L. Le Marchand (Loic); J. Simard (Jacques); M.S. Goldberg (Mark); F. Labrèche (France); M. Dumont (Martine); S.-H. Teo; C.H. Yip (Cheng Har); M.-H. See (Mee-Hoong); B.K. Cornes (Belinda); C.-Y. Cheng (Ching-Yu); M.K. Ikram (Kamran); V. Kristensen (Vessela); W. Zheng (Wei); S.L. Halverson (Sandra L.); M. Shrubsole (Martha); J. Long (Jirong); R. Winqvist (Robert); K. Pykäs (Katri); A. Jukkola-Vuorinen (Arja); S. Kauppila (Saila); I.L. Andrulis (Irene); J.A. Knight (Julia); G. Glendon (Gord); S. Tchatchou (Sandrine); P. Devilee (Peter); R.A.E.M. Tollenaar (Rob); C.M. Seynaeve (Caroline); C.J. van Asperen (Christi); M. García-Closas (Montserrat); J.D. Figueroa (Jonine); S.J. Chanock (Stephen); J. Lissowska (Jolanta); K. Czene (Kamila); D. Klevebring (Daniel); H. Darabi (Hatef); M. Eriksson (Mikael); M.J. Hooning (Maartje); A. Hollestelle (Antoinette); J.W.M. Martens (John W. M.); J.M. Collee (Margriet); P. Hall (Per); J. Li (Jingmei); K. Humphreys (Keith); X.-O. Shu (Xiao-Ou); W. Lu (Wei); Y.-T. Gao (Yu-Tang); H. Cai (Hui); A. Cox (Angela); S.S. Cross (Simon); M.W.R. Reed (Malcolm); W.J. Blot (William); L.B. Signorello (Lisa B.); Q. Cai (Qiuyin); M. Shah (Mitul); M. Ghoussaini (Maya); D. Kang (Daehee); J.-Y. Choi (J.); S.K. Park (Sue); D-Y. Noh (Dong-Young); M. Hartman (Mikael); X. Miao; W.-Y. Lim (Wei-Yen); A. Tang (Anthony); U. Hamann (Ute); D. Torres (Diana); A. Jakubowska (Anna); J. Lubinski (Jan); K. Jaworska (Katarzyna); K. Durda (Katarzyna); S. Sangrajrang (Suleeporn); V. Gaborieau (Valerie); P. Brennan (Paul); J.D. McKay (James); C. Olswold (Curtis); S. Slager (Susan); A.E. Toland (Amanda); D. Yannoukakos (Drakoulis); C-Y. Shen (Chen-Yang); P.-E. Wu (Pei-Ei); J-C. Yu (Jyh-Cherng); M.-F. Hou (Ming-Feng); A.J. Swerdlow (Anthony ); A. Ashworth (Alan); N. Orr (Nick); M. Jones (Michael); G. Pita (G.); M.R. Alonso (M Rosario); N. Álvarez (Nuria); D. Herrero (Daniel); D.C. Tessier (Daniel C.); D. Vincent (Daniel); F. Bacot (Francois); C. Luccarini (Craig); C. Baynes (Caroline); S. Ahmed (Shahana); S. Healey (Sue); M. Brown (Melissa); B.A.J. Ponder (Bruce A.J.); G. Chenevix-Trench (Georgia); D. Thompson (Deborah); S.L. Edwards (Stacey); D.F. Easton (Douglas); A.M. Dunning (Alison); J.D. French (Juliet)

    2015-01-01

    textabstractGenome-wide association studies (GWASs) have revealed SNP rs889312 on 5q11.2 to be associated with breast cancer risk in women of European ancestry. In an attempt to identify the biologically relevant variants, we analyzed 909 genetic variants across 5q11.2 in 103,991 breast cancer indiv

  17. Identification of a haplotype block in the 5q31 cytokine gene cluster associated with the susceptibility to severe malaria

    Tsuchiya Naoyuki

    2009-10-01

    Full Text Available Abstract Background It has been previously demonstrated that a single nucleotide polymorphism (SNP in the IL13 promoter region, IL13 -1055T>C (rs1800925, was associated with susceptibility to severe malaria in Thais. In the present study, fine association mapping for a cytokine gene cluster including IL4, IL5, and IL13 on chromosome 5q31 was conducted using the same malaria subjects to refine the region containing a primary variant or a haplotype susceptible to severe malaria. Methods A total of 82 SNPs spanning 522 kb of the 5q31 region were analysed in 368 patients with Plasmodium falciparum malaria (203 mild malaria and 165 severe malaria patients. Results Only rs1881457 located in the promoter region of IL13, which is in linkage disequilibrium with rs1800925 (r2 = 0.73, showed a significant association with severe malaria after adjusting for multiple testing (P = 0.046 by permutation test. This SNP was in a haplotype block spanning 97 kb (from rs2069812 to rs2240032. The detected haplotype block contained the RAD50 gene and the promoter of IL13, but not the other genes. Conclusion A haplotype block in which a primary polymorphism associated with severe malaria is likely to be encoded was identified in Thai malaria patients.

  18. TP53 and MDM2 single nucleotide polymorphisms influence survival in non-del(5q) myelodysplastic syndromes.

    McGraw, Kathy L; Cluzeau, Thomas; Sallman, David A; Basiorka, Ashley A; Irvine, Brittany A; Zhang, Ling; Epling-Burnette, P K; Rollison, Dana E; Mallo, Mar; Sokol, Lubomir; Solé, Francesc; Maciejewski, Jaroslaw; List, Alan F

    2015-10-27

    P53 is a key regulator of many cellular processes and is negatively regulated by the human homolog of murine double minute-2 (MDM2) E3 ubiquitin ligase. Single nucleotide polymorphisms (SNPs) of either gene alone, and in combination, are linked to cancer susceptibility, disease progression, and therapy response. We analyzed the interaction of TP53 R72P and MDM2 SNP309 SNPs in relationship to outcome in patients with myelodysplastic syndromes (MDS). Sanger sequencing was performed on DNA isolated from 208 MDS cases. Utilizing a novel functional SNP scoring system ranging from +2 to -2 based on predicted p53 activity, we found statistically significant differences in overall survival (OS) (p = 0.02) and progression-free survival (PFS) (p = 0.02) in non-del(5q) MDS patients with low functional scores. In univariate analysis, only IPSS and the functional SNP score predicted OS and PFS in non-del(5q) patients. In multivariate analysis, the functional SNP score was independent of IPSS for OS and PFS. These data underscore the importance of TP53 R72P and MDM2 SNP309 SNPs in MDS, and provide a novel scoring system independent of IPSS that is predictive for disease outcome. PMID:26416416

  19. Miller-Dieker syndrome associated with duplication of 17p13.3 confirmed by fluorescence in situ hybridization (FISH)

    Li, S.; Tuck-Muller, C.M.; Martinez, J.E. [Univ. of South Alabama, Mobile, AL (United States)] [and others

    1994-09-01

    Miller-Dieker syndrome is characterized by profound mental retardation, craniofacial abnormalities, and lissencephaly (smooth brain). Microscopic or submicroscopic deletions of the 17p13.3 region have been reported in Miller-Dieker patients. We report a patient with this syndrome in whom a duplication of the 17p13.3 region was detected by FISH. The 9-year-old female proband was referred because of features of Miller-Dieker syndrome: microcephaly, profound psychomotor retardation, seizures, characteristic facies, and lissencephaly shown by MRI studies. High-resolution G-banding failed to demonstrate an abnormality in chromosome 17. However, FISH analysis with the DNA probe (Oncor No. 5101) specific for Miller-Dieker region of chromosome 17p13.3 demonstrated duplication of this segment instead of the classic deletion. We know of no other report of Miller-Dieker syndrome associated with duplication of 17p13.3. The family study revealed normal chromosomes in both parents by cytogenetic and FISH analysis. Our investigation suggests that duplications, as well as deletions, of the 17p13.3 region are associated with the Miller-Dieker syndrome. The presence of deletions or duplications of the same chromosomal region in patients with features of Miller-Dieker syndrome suggests that its pathogenesis may be due to gene dosage effects.

  20. Monitoring Observations of the Jupiter-Family Comet 17P/Holmes during 2014 Perihelion Passage

    Kwon, Yuna Grace; Hanayama, Hidekazu; Kuroda, Daisuke; Honda, Satoshi; Takahashi, Jun; Kim, Yoonyoung; Lee, Myung Gyoon; Choi, Young-Jun; Kim, Myung-Jin; Vaubaillon, Jeremie J; Miyaji, Takeshi; Yanagisawa, Kenshi; Yoshida, Michitoshi; Ohta, Kouji; Kawai, Nobuyuki; Fukushima, Hideo; Watanabe, Jun-ichi

    2015-01-01

    We performed a monitoring observation of a Jupiter-Family comet, 17P/Holmes, during its 2014 perihelion passage to investigate its secular change in activity. The comet has drawn the attention of astronomers since its historic outburst in 2007, and this occasion was its first perihelion passage since then. We analyzed the obtained data using aperture photometry package and derived the Afrho parameter, a proxy for the dust production rate. We found that Afrho showed asymmetric properties with respect to the perihelion passage: it increased moderately from 100 cm at the heliocentric distance r_h=2.6-3.1 AU to a maximal value of 185 cm at r_h = 2.2 AU (near the perihelion) during the inbound orbit, while dropping rapidly to 35 cm at r_h = 3.2 AU during the outbound orbit. We applied a model for characterizing dust production rates as a function of r_h and found that the fractional active area of the cometary nucleus had dropped from 20%-40% in 2008-2011 (around the aphelion) to 0.1%-0.3% in 2014-2015 (around the...

  1. Peculiar Near-Nucleus Outgassing of Comet 17P/Holmes During Its 2007 Outburst

    Qi, Chunhua; Jewitt, David; Gurwell, Mark A; Wilner, David J

    2014-01-01

    We present high angular resolution Submillimeter Array observations ofthe outbursting Jupiter family comet 17P/Holmes on 2007 October 26-29, achieving a spatial resolution of 2.5", or ~3000 km at the comet distance. The observations resulted in detections of the rotational lines CO 3-2, HCN 4-3, H$^{13}$CN 4-3, CS 7-6, H$_2$CO 3$_{1,2}$-2$_{1,1}$, H$_2$S 2$_{2,0}$-2$_{1,1}$, and multiple CH$_3$OH lines, along with the associated dust continuum at 221 and 349 GHz. The continuum has a spectral index of 2.7$\\pm$0.3, slightly steeper than blackbody emission from large dust particles. From the imaging data, we identify two components in the molecular emission. One component is characterized by a relatively broad line width (~1 km s$^{-1}$ FWHM) exhibiting a symmetric outgassing pattern with respect to the nucleus position. The second component has a narrower line width (<0.5 km s$^{-1}$ FWHM) with the line center red-shifted by 0.1-0.2 km s$^{-1}$ (cometocentric frame), and shows a velocity shift across the nuc...

  2. Outburst of Comet 17P/Holmes Observed With The Solar Mass Ejection Imager

    Li, Jing

    2010-01-01

    We present time-resolved photometric observations of Jupiter family comet 17P/Holmes during its dramatic outburst of 2007. The observations, from the orbiting Solar Mass Ejection Imager (SMEI), provide the most complete measure of the whole-coma brightness, free from the effects of instrumental saturation and with a time-resolution well-matched to the rapid brightening of the comet. The lightcurve is divided into two distinct parts. A rapid rise between the first SMEI observation on UT 2007 October 24 06h 37m (mid-integration) and UT 2007 October 25, is followed by a slow decline until the last SMEI observation on UT 2008 April 6 22h 16m (mid-integration). We find that the rate of change of the brightness is reasonably well-described by a Gaussian function having a central time of UT 2007 October 24.54+/-0.01 and a full-width-at-half-maximum 0.44+/-0.02 days. The maximum rate of brightening occurs some 1.2 days after the onset of activity. At the peak the scattering cross-section grows at 1070+/-40 km^2/s whi...

  3. Identification, by homozygosity mapping, of a novel locus for autosomal recessive congenital ichthyosis on chromosome 17p, and evidence for further genetic heterogeneity.

    Krebsová, A; Küster, W; Lestringant, G G; Schulze, B; Hinz, B; Frossard, P M; Reis, A; Hennies, H C

    2001-07-01

    Autosomal recessive congenital ichthyosis (ARCI) comprises a group of severe disorders of keratinization, characterized by variable erythema and skin scaling. It is known for its high degree of genetic and clinical heterogeneity. Mutations in the gene for keratinocyte transglutaminase (TGM1) on chromosome 14q11 were shown in patients with ARCI, and a second locus was described, on chromosome 2q, in families from northern Africa. Three other loci for ARCI, on chromosomes 3p and 19p, were identified recently. We have embarked on a whole-genome scan for further loci for ARCI in four families from Germany, Turkey, and the United Arab Emirates. A novel ARCI locus was identified on chromosome 17p, between the markers at D17S938 and D17S1856, with a maximum LOD score of 3.38, at maximum recombination fraction 0.00, at D17S945, under heterogeneity. This locus is linked to the disease in the Turkish family and in the German family. Extensive genealogical studies revealed that the parents of the German patients with ARCI were eighth cousins. By homozygosity mapping, the localization of the gene could then be refined to the 8.4-cM interval between D17S938 and D17S1879. It could be shown, however, that ARCI in the two Arab families is linked neither to the new locus on chromosome 17p nor to one of the five loci known previously. Our findings give evidence of further genetic heterogeneity that is not linked to distinctive phenotypes. PMID:11398099

  4. Lenalidomide as a disease-modifying agent in patients with del(5q) myelodysplastic syndromes: linking mechanism of action to clinical outcomes

    Giagounidis, Aristoteles; Mufti, Ghulam J.; Fenaux, Pierre; Germing, Ulrich; List, Alan; MacBeth, Kyle J.

    2013-01-01

    Deletion of the long arm of chromosome 5, del(5q), is the most prevalent cytogenetic abnormality in patients with myelodysplastic syndromes (MDS). In isolation, it is traditionally associated with favorable prognosis compared with other subtypes of MDS. However, owing to the inherent heterogeneity of the disease, prognosis for patients with del(5q) MDS is highly variable depending on the presence of factors such as additional chromosomal abnormalities, >5 % blasts in the bone marrow (BM), or ...

  5. Detection of Remnant Dust Cloud Associated with the 2007 Outburst of 17P/Holmes

    Ishiguro, Masateru; Sarugaku, Yuki; Kuroda, Daisuke; Hanayama, Hidekazu; Kim, Yoonyoung; Kwon, Yuna G.; Maehara, Hiroyuki; Takahashi, Jun; Terai, Tsuyoshi; Usui, Fumihiko; Vaubaillon, Jeremie J.; Morokuma, Tomoki; Kobayashi, Naoto; Watanabe, Jun-ichi

    2016-01-01

    This article reports a new optical observation of 17P/Holmes one orbital period after the historical outburst event in 2007. We detected not only a common dust tail near the nucleus but also a long narrow structure that extended along the position angle 274.°6 ± 0.°1 beyond the field of view (FOV) of the Kiso Wide Field Camera, i.e., >0.°2 eastward and >2.°0 westward from the nuclear position. The width of the structure decreased westward with increasing distance from the nucleus. We obtained the total cross section of the long extended structure in the FOV, CFOV = (2.3 ± 0.5) × 1010 m2. From the position angle, morphology, and mass, we concluded that the long narrow structure consists of materials ejected during the 2007 outburst. On the basis of the dynamical behavior of dust grains in the solar radiation field, we estimated that the long narrow structure would be composed of 1 mm-1 cm grains having an ejection velocity of >50 m s-1. The velocity was more than one order of magnitude faster than that of millimeter-centimeter grains from typical comets around a heliocentric distance rh of 2.5 AU. We considered that sudden sublimation of a large amount of water-ice (≈1030 mol s-1) would be responsible for the high ejection velocity. We finally estimated a total mass of MTOT = (4-8) × 1011 kg and a total kinetic energy of ETOT = (1-6) × 1015 J for the 2007 outburst ejecta, which are consistent with those of previous studies that were conducted soon after the outburst.

  6. 2007 OUTBURST OF 17P/HOLMES: THE ALBEDO AND THE TEMPERATURE OF THE DUST GRAINS

    Based on optical and infrared observations, we study the albedo and the temperature of the dust grains associated with the spectacular 2007 outburst of Jupiter-family comet 17P/Holmes. We found that the albedo at the solar phase angle ∼160 was 0.03-0.12. While the color temperature around 3-4 μm was 360 ± 40 K, the color temperature at 12.4 μm and 24.5 μm was ∼200 K, which is consistent with that of a blackbody. We studied the equilibrium temperature of the dust grains at 2.44 AU and found that the big discrepancy in the temperature was caused by the heterogeneity in particle size, that is, hotter components consist of submicron absorbing grains whereas colder components consist of large (∼>1 μm) grains. The contemporaneous optical and mid-infrared observations suggest that the albedo and the temperature could decrease within ∼ 3 days after the outburst and stabilized at typical values of the other comets. We estimated the total mass injected into the coma by the outburst on the basis of the derived albedo and the optical magnitude for the entire dust cloud, and found that at least 4 x 1010 kg (equivalent to a few meter surface layer) was removed by the initial outburst event. The derived mass suggests that the outburst is explainable by neither the exogenetic asteroidal impact nor water ice sublimation driven by solar irradiation, but by an endogenic energy source. We conclude that the outburst was triggered by the energy sources several meters or more below the nuclear surface.

  7. $^3P_2$ Superfluids Are Topological

    Mizushima, Takeshi

    2016-01-01

    We clarify the topology of the $^3P_2$ superfluidity which is expected to be realized in the cores of neutron stars and cubic odd-parity superconductors. The phase diagram includes the unitary uniaxial/biaxial nematic phases and nonunitary ferromagnetic and cyclic phases. We here show that the low-energy structures of all the phases are governed by different types of topologically protected gapless fermionic excitations: Surface Majorana fermions in nematic phases, single itinerant Majorana fermion in the ferromagnetic phase, and a quartet of itinerant Majorana fermions in the cyclic phase. Using the superfluid Fermi liquid theory, we also demonstrate that dihedral-two and -four biaxial nematic phases are thermodynamically favored in the weak coupling limit under a magnetic field. The mass acquisition of surface Majorana fermions in nematic phases is subject to symmetry.

  8. Detailed deletion mapping in sporadic breast cancer at chromosomal region 17p13 distal to the TP53 gene: association with clinicopathological parameters.

    Seitz, S; Poppe, K; Fischer, J; Nothnagel, A; Estévez-Schwarz, L; Haensch, W; Schlag, P M; Scherneck, S

    2001-07-01

    Chromosome 17p is among the most frequently deleted regions in a variety of human malignancies including breast cancer. This study has further refined the localization of a putative tumour suppressor gene (TSG) at 17p13 distal to the TP53 gene in breast carcinomas. It was found that 73% (37 of 51) of the breast tumours exhibited loss of heterozygosity (LOH) at one or more loci at 17p13. The allelic loss patterns of these tumours suggest the presence of at least seven commonly deleted regions on 17p13. The three most frequently deleted regions were mapped at chromosomal location 17p13.3-17p13.2 between the markers D17S831 and D17S1845 (56% LOH), at 17p13.1 between D17S1810 and D17S1832 (53% LOH), and at 17p13.1 between D17S938 and TP53 (55% LOH). A significant correlation was found between loss at 17p13 and tumour grade, size, proliferative activity, and oestrogen receptor (ER) status. Losses at 17p13 were seen more frequently in large and poorly differentiated tumours with high proliferative activity. These data support and extend previous reports on the presence of a putative TSG(s) at chromosomal region 17p13 distal to the TP53 gene and show that different subsets of LOH are associated with more aggressive tumour behaviour. PMID:11439364

  9. A new microduplication syndrome encompassing the region of the Miller-Dieker (17p13 deletion) syndrome

    Roos, L; Jønch, A E; Kjaergaard, S;

    2009-01-01

    cases with this duplication. METHODS: Multiplex ligation-dependent probe amplification (MLPA) or array-CGH was used to diagnose three developmentally delayed children with duplications of 17p13. The duplications were characterised further using Agilent array technology, revealing duplication sizes from...

  10. PECULIAR NEAR-NUCLEUS OUTGASSING OF COMET 17P/HOLMES DURING ITS 2007 OUTBURST

    Qi, Chunhua; Gurwell, Mark A.; Wilner, David J. [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, MS 42, Cambridge, MA 02138 (United States); Hogerheijde, Michiel R. [Leiden Observatory, Leiden University, P.O. Box 9513, 2300 RA Leiden (Netherlands); Jewitt, David, E-mail: cqi@cfa.harvard.edu [Department of Earth, Planetary and Space Sciences and Department of Physics and Astronomy, University of California at Los Angeles, 595 Charles Young Drive East, Los Angeles, CA 90095 (United States)

    2015-01-20

    We present high angular resolution Submillimeter Array observations of the outbursting Jupiter family comet 17P/Holmes on 2007 October 26-29, achieving a spatial resolution of 2.''5, or ∼3000 km at the comet distance. The observations resulted in detections of the rotational lines CO 3-2, HCN 4-3, H{sup 13}CN 4-3, CS 7-6, H{sub 2}CO 3{sub 1,} {sub 2}-2{sub 1,} {sub 1}, H{sub 2}S 2{sub 2,} {sub 0}-2{sub 1,} {sub 1}, and multiple CH{sub 3}OH lines, along with the associated dust continuum at 221 and 349 GHz. The continuum has a spectral index of 2.7 ± 0.3, slightly steeper than blackbody emission from large dust particles. From the imaging data, we identify two components in the molecular emission. One component is characterized by a relatively broad line width (∼1 km s{sup –1} FWHM) exhibiting a symmetric outgassing pattern with respect to the nucleus position. The second component has a narrower line width (<0.5 km s{sup –1} FWHM) with the line center redshifted by 0.1-0.2 km s{sup –1} (cometocentric frame), and shows a velocity shift across the nucleus position with the position angle gradually changing from 66° to 30° within the four days of observations. We determine distinctly different CO/HCN ratios for each of the components. For the broad-line component we find CO/HCN < 7, while in the narrow-line component, CO/HCN = 40 ± 5. We hypothesize that the narrow-line component originates from the ice grain halo found in near-nucleus photometry, believed to be created by sublimating recently released ice grains around the nucleus during the outburst. In this interpretation, the high CO/HCN ratio of this component reflects the more pristine volatile composition of nucleus material released in the outburst.

  11. In patients with myelodysplastic syndromes with del(5q), factors other than age and sex contribute to the prognostic advantage, which diminishes over time.

    Lauseker, Michael; Schemenau, Jennifer; Strupp, Corinna; Kündgen, Andrea; Gattermann, Norbert; Hasford, Joerg; Germing, Ulrich

    2015-09-01

    This study aimed to determine the extent to which the prognostic advantage of myelodysplastic syndromes (MDS) with del(5q) is due to the more favourable age and sex distribution of patients in that group when compared to other MDS subtypes. A total of 1912 MDS patients from the Duesseldorf registry with less than 5% blasts in the bone marrow were evaluable and had complete covariates. As endpoints, overall survival and progression to acute myeloid leukaemia (AML) were considered. Cox models were computed for both outcomes. A multivariate Cox model for survival confirmed higher age and male sex as risk factors. In addition, we found a survival advantage of 9·1 years for MDS del(5q) patients compared to refractory cytopenia with unilineage dysplasia, while the survival advantage of MDS del(5q) over refractory cytopenia with multilineage dysplasia was 18·6 years. Considering progression to AML, we did not find any significant differences between the World Health Organization classification subtypes. Our analyses show that the higher survival probabilities of MDS del(5q) patients are not only due to age and sex, although higher age and male sex were also important risk factors. Interestingly, it seems that the survival advantage of MDS del(5q) decreases over time. PMID:25960152

  12. Identification of an unbalanced cryptic translocation t(9;17)(q34.3;p13.3) in a child with dysmorphic features

    Estop, A M; Mowery-Rushton, P A; Cieply, K M; Kochmar, S J; Sherer, C R; Clemens, M; Surti, U; McPherson, E.

    1995-01-01

    We report a case of an unbalanced cryptic telomeric translocation 46,XY,der(17),t(9;17)(q34.3;p13.3) in a boy with dysmorphic features and developmental delay. The proband had intrauterine growth retardation, postnatal short stature, and mild microcephaly. Magnetic resonance imaging showed incomplete myelination, but no evidence of lissencephaly. Cytogenetic analysis of the proband's peripheral blood showed an abnormal 17p. Fluorescence in situ hybridisation (FISH) with a Miller-Dieker cosmid...

  13. Irradiation-induced softening of Ni3P and (Ni, Fe, Cr)3P alloys

    Production of amorphous alloys by solid state reactions (SSR) has attracted much interest during the last few years. One of the methods to induce such a reaction is the irradiation of suitable crystalline alloys by fast particles. Examination of this kind of SSR in M3P type of brazing alloys (M: Metal) is attractive because of the following reason: In brazed joints of candidate structural materials like 316L stainless steel for applications in fusion reactors, crystalline intermetallic phases have been detected which are unstable relative to the amorphous state when irradiated at moderate temperatures with fast particles. It is expected that the transition to the amorphous state is accompanied by changes of the mechanical properties, which are of fundamental interest in this context. Until now, only a few studies on the evolution of mechanical properties during amorphization have been performed. Measurements of microhardness of the crystalline and the corresponding amorphous phase do not exist to the authors knowledge. In this communication, the authors present results on changes of microhardness, due to amorphization by fast ions. The measurements have been performed on a model alloy Ni3P and on the brazed joint of stainless steel 316L, containing M3P (M: Ni, Fe, Cr) as one of the phases. Though microhardness is not a fundamental property of materials, it is a manifestation of several related properties, such as yield stress, ductility, work-hardening, elastic modulus and residual stress states. It represents a resistance for indentation and is, therefore, appropriate for comparative purposes

  14. Identification of a novel sarcoglycan gene at 5q33 encoding a sarcolemmal 35 kDa glycoprotein.

    Nigro, V; Piluso, G; Belsito, A; Politano, L; Puca, A A; Papparella, S; Rossi, E; Viglietto, G; Esposito, M G; Abbondanza, C; Medici, N; Molinari, A M; Nigro, G; Puca, G A

    1996-08-01

    Mutations in any of the genes encoding the alpha, beta or gamma-sarcoglycan components of dystrophin-associated glycoproteins result in both sporadic and familial cases of either limb-girdle muscular dystrophy or severe childhood autosomal recessive muscular dystrophy. The collective name 'sarcoglycanopathies' has been proposed for these forms. We report the identification of a fourth member of the human sarcoglycan family. We named this novel cDNA delta-sarcoglycan. Its mRNA expression is abundant in striated and smooth muscles, with a main 8 kb transcript, encoding a predicted basic transmembrane glycoprotein of 290 amino acids. Antibodies specifically raised against this protein recognized a single band at 35 kDa on western blots of human and mouse muscle. Immunohistochemical staining revealed a unique sarcolemmal localization. FISH, radiation hybrid and YAC mapping concordantly linked the delta-sarcoglycan gene to 5q33, close to D5S487 and D5S1439. The gene spans at least 100 kb and is composed of eight exons. The identification of a novel sarcoglycan component modifies the current model of the dystrophin-glycoprotein complex. PMID:8842738

  15. Complex chromosome 17p rearrangements associated with low-copy repeats in two patients with congenital anomalies

    Vissers, L. E. L. M.; Stankiewicz, P; Yatsenko, S.A.; Crawford, E.; Creswick, H.; Proud, V K; de Vries, B.B.A.; Pfundt, R.; Marcelis, C.L.M.; Zackowski, J.; Bi, W; van Kessel, A. Geurts; Lupski, J R; Veltman, J.A.

    2007-01-01

    Recent molecular cytogenetic data have shown that the constitution of complex chromosome rearrangements (CCRs) may be more complicated than previously thought. The complicated nature of these rearrangements challenges the accurate delineation of the chromosomal breakpoints and mechanisms involved. Here, we report a molecular cytogenetic analysis of two patients with congenital anomalies and unbalanced de novo CCRs involving chromosome 17p using high-resolution array-based comparative genomic ...

  16. A novel unbalanced de novo translocation der(5t(4;5(q26;q21.1 in adult T-cell precursor lymphoblastic leukemia

    Kjeldsen Eigil

    2012-05-01

    Full Text Available Abstract We here describe a novel unbalanced de novo translocation der(5t(4;5(q26;q21.1 in a 39-year-old male diagnosed with acute T-cell lymphoblastic leukemia. Bone marrow (BM was massively infiltrated with 85 % highly proliferative polymorphic T-cell precursors. Immunologically, the malignant cells stained positive for CD7, CD34, intracytoplasmic CD3+, TdT + and negative for CD3 and CD5. G-banded chromosome analysis of BM cells showed the normal karyotype 46,XY[25] whereas BAC-based aCGH analysis revealed partial gain of 4q and partial loss of 5q. Multicolor karyotyping confirmed the presence of an unbalanced der(5t(4;5 as the sole structural abnormality. Subsequent high-resolution oligonucleotide-based aCGH analysis showed that the der(5t(4;5(q26;q21.1 resulted in partial trisomy of 4q26qter (117,719,015-190,613,014 and partial monosomy of 5q21.1qter (100,425,442-180,857,866 and that there was no indication of any gene disruptions resulting from the breakages. Interphase FISH analysis using BAC-based specific probes for 4q26 and 5q21.1 confirmed the breakpoints and revealed approximately 80 % abnormal cells accordingly. At 4q26 the MIR1973 gene is located centromeric to the breakpoint in the copy number neutral region and the TRAM1L1 gene is located within the gained region. At 5q21.1 the genes ST8SIA4 and MIR548p are located centromeric to the breakpoint and no known genes up to approximately 1 Mb telomeric to the breakpoint in the copy number loss region. Interestingly, only the gene ST8SIA4 at 5q21.1 have been implicated in T-cell regulation as it encodes one of the key enzymes for polysialysation of surface proteins on dendritic cells which are important regulators for T-cell proliferation. The der(5t(4;5 is thought to play a crucial role in the pathogenesis of acute T-ALL due to either gain of 4q, the loss of 5q, or deregulation of genes in proximity to the breakpoints.

  17. Spectra and oscillator strengths of 3p63d9-3p53d10 and 3p63d9-3p63d84p transitions for cobalt-like Sn23+ ion

    Chen Ming-Lun; Yu Xiao-Guang

    2009-01-01

    This paper calculates the spectra and oscillator strengths for highly ionized cobalt-like Sn23+ ions 3p63d9-3p53d10,3p63d9 - 3p63d84p transitions by using a multi-configuration self-consistent field method program together with the proposed fitting formula. The calculations have a good agreement with observations.

  18. Differential cross section of metastable Ne(3P0 and 3P2) scattered from ground state neon

    The differential elastic cross section for metastable Ne(3P0 and 3P2) from ground state Ne at collision energies between 70 and 150 meV have been measured. With a CW-dye laser it was possible to separate the cross sections for Ne(3P0) + Ne and Ne(3P2) + Ne and for the energy exchange process 20Ne* + 22Ne → 20Ne + 22Ne*. (Auth.)

  19. Normal female carrier and affected male half-sibs with t(X;5)(q13;p15). Location of a gene determining male genital development.

    Callen, D F; Sutherland, G R

    1986-07-01

    A unique family in which half-brothers have a maternally derived t(X;5)(q13;p15) and similar genital malformations is described. This family provides evidence for a gene required for male genital development located at Xq13. PMID:3757297

  20. 31 CFR 30.5 - Q-5: How does a TARP recipient comply with the requirements under § 30.4 (Q-4) of this part that...

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Q-5: How does a TARP recipient comply... recipient? 30.5 Section 30.5 Money and Finance: Treasury Office of the Secretary of the Treasury TARP STANDARDS FOR COMPENSATION AND CORPORATE GOVERNANCE § 30.5 Q-5: How does a TARP recipient comply with...

  1. Switch in FGFR3 and -4 expression profile during human renal development may account for transient hypercalcemia in patients with Sotos syndrome due to 5q35 microdeletions

    Mutsaers, H.A.M.; Levtchenko, E.N.; Martinerie, L.; Pertijs, J.C.L.M.; Allegaert, K.; Devriendt, K.; Masereeuw, R.; Monnens, L.A.; Lombes, M.

    2014-01-01

    CONTEXT: Sotos syndrome is a rare genetic disorder with a distinct phenotypic spectrum including overgrowth and learning difficulties. Here we describe a new case of Sotos syndrome with a 5q35 microdeletion, affecting the fibroblast growth factor receptor 4 (FGFR4) gene, presenting with infantile hy

  2. Allelic methylation levels of the noncoding VTRNA2-1 located on chromosome 5q31.1 predict outcome in AML

    Treppendahl, Marianne Bach; Qiu, Xiangning; Søgaard, Alexandra;

    2012-01-01

    Deletions of chromosome 5q are associated with poor outcomes in acute myeloid leukemia (AML) suggesting the presence of tumor suppressor(s) at the locus. However, definitive identification of putative tumor suppressor genes remains controversial. Here we show that a 106-nucleotide noncoding RNA...

  3. Differential cross section for Ne(3P0) and Ne(3P2) scattered from ground state neon

    The differential elastic cross sections for electronically excited Ne(3P0) and Ne(3P2) from ground state neon atoms at thermal collision energies have been measured separately. Potentials for Ne(3P0)+Ne could be determined and the height of the intermediate potential maxima for Ne(3P2)+Ne could be estimated to 25 meV. By preparing Ne(3P2) in special Msub(J) states scattering in single Ω molecular states could be observed. (Auth.)

  4. Differential cross section for Ne(3P0) and Ne(3P2) scattered from ground state neon

    The differential elastic cross sections for electronically exited Ne(3P0) and Ne(3P2) from ground state neon atoms at thermal collision energies have been measured separately. Potentials for Ne(3P0)+Ne could be determined and the height of the intermediate potential maxima for Ne(3P2)+Ne could be estimated to 25 meV. By preparing Ne(3P2) in special Msub(J) states scattering in single Ω molecular states could be observed. (Author)

  5. Interferometric mapping of the 3.3-mm continuum emission of comet 17P/Holmes after its 2007 outburst

    Boissier, J.; Bockelée-Morvan, D.; Biver, N; Crovisier, J; Lellouch, E.; Moreno, R.; Zakharov, V.

    2012-01-01

    Comet 17P/Holmes underwent a dramatic outburst in October 2007, caused by the sudden fragmentation of its nucleus and the production of a large quantity of grains scattering sunlight. We report on 90 GHz continuum observations carried out with the IRAM Plateau de Bure interferometer on 27.1 and 28.2 October 2007 UT, i.e., 4-5 days after the outburst. These observations probed the thermal radiation of large dust particles, and therefore provide the best constraints on the mass in the ejecta de...

  6. Structural analysis of a hepatitis B virus genome integrated into chromosome 17p of a human hepatocellular carcinoma

    Hepatitis B virus (HBV) is clearly a factor in the development of hepatocellular carcinoma, but its mechanism of action remains obscure. One possibility is that the HBV integration event alters the expression of a nearby growth-regulatory cellular gene. A 9-kilobase (kb) DNA fragment containing an HBV insert plus flanking cellular sequences was cloned from a hepatoma specimen from Shanghai, People's Republic of China. Restriction mapping of the insert revealed a large inverted repeat structure consisting of both viral sequences (encompassing all of the core and pre-S regions and portions of the X and S genes) and at least 3 kb of unique cellular sequences. The virus-cell junction mapped 11 nucleotides from the DRI region, in a position within the HBV X gene and included in the cohesive overlap region. A probe generated from 1.0 kb of the flanking cellular DNA mapped the viral insert to chromosome 17 in the region designated 17p11.2-17p12, which is near the human proto-oncogene p53. Sequence data from a portion of the flanking cellular DNA revealed a stretch of approximately 70 base pairs that showed highly significant homology with a conserved region of a number of functional mammalian DNA, including the human autonomously replicating sequence 1 (ASRI)

  7. A gene for autosomal dominant progressive cone dystrophy (CORD5) maps to chromosome 17p12-p13

    Balciuniene, J.; Holmgren, G.; Forsman, K. [University Hospital, Umea (Sweden)] [and others

    1995-11-20

    Inherited retinal dystrophy is a common cause of visual impairment. Cone dystrophy affects the cone function and is manifested as progressive loss of the central vision, defective color vision, and photophobia. Linkage was demonstrated between progressive cone dystrophy (CORD5) and genetic markers on chromosome 17p12-p13 in a five-generation family. Multipoint analysis gave a maximum lod score of 7.72 at the marker D17S938. Recombinant haplotypes in the family suggest that the cone dystrophy locus is located in a 25-cM interval between the markers D17S926/D17S849 and D17S804/D17S945. Furthermore, one recombination was detected between the disease locus and a microsatellite marker in the candidate gene RCV1, encoding the retinal protein recoverin. Two additional candidate genes encoding retinal guanylate cyclase (GUC2D) and pigment epithelium-derived factor (PEDF) are located at 17p13.1. Moreover, loci for retinitis pigmentosa and Leber congenital amaurosis have been mapped to the same region. Identification of the cone dystrophy locus may be of importance not only for identifying functional genes in the cone system, but also for identifying genes for other retinal disorders. 34 refs., 3 figs., 2 tabs.

  8. Gene Expression Meta-Analysis identifies Cytokine Pathways and 5q Aberrations involved in Metastasis of ERBB2 Amplified and Basal Breast Cancer

    Thomassen, Mads; Tan, Qihua; Burton, Mark;

    2013-01-01

    show that high expression of 5q14 genes and low levels of TNFR2 pathway genes were associated with poor survival in basal-like cancers. Furthermore, low expression of 5q33 genes and interleukin-12 pathway genes were associated with poor outcome exclusively in ERBB2-like tumors. Conclusion: The......Background: Breast tumors have been described by molecular subtypes characterized by pervasively different gene expression profiles. The subtypes are associated with different clinical parameters and origin of precursor cells. However, the biological pathways and chromosomal aberrations that differ...... subgroups impact metastasis. Results: We have scrutinized publicly available gene expression datasets and identified molecular subtypes in 1,394 breast tumors with outcome data. By analysis of chromosomal regions and pathways using “Gene set enrichment analysis” followed by a meta-analysis, we identified...

  9. Cooling of Neutron Stars and 3P_2 neutron gap

    Grigorian, H.; Voskresensky, D.N.(National Research Nuclear University (MEPhI), Moscow, 115409, Russia)

    2005-01-01

    We study the dependence of the cooling of isolated neutron stars on the magnitude of the $3P_2$ neutron gap. It is demonstrated that our ``Nuclear medium cooling scenario'' is in favor of a suppressed value of the $3P_2$ neutron gap.

  10. 3p - 3d intershell interaction in Cr

    The photoemission of Cr films deposited under UHV conditions has been investigated in the photon energy range from 30 eV to 230 eV. The 3p - 3d intershell interaction gives rise to a strong maximum in the 3d partial yield above the 3p threshold. (orig.) 891 KBE/orig. 892 RDG

  11. A mitotic recombination map proximal to the APC locus on chromosome 5q and assessment of influences on colorectal cancer risk

    Clark Susan

    2009-06-01

    Full Text Available Abstract Background Mitotic recombination is important for inactivating tumour suppressor genes by copy-neutral loss of heterozygosity (LOH. Although meiotic recombination maps are plentiful, little is known about mitotic recombination. The APC gene (chr5q21 is mutated in most colorectal tumours and its usual mode of LOH is mitotic recombination. Methods We mapped mitotic recombination boundaries ("breakpoints" between the centromere (~50 Mb and APC (~112 Mb in early colorectal tumours. Results Breakpoints were non-random, with the highest frequency between 65 Mb and 75 Mb, close to a low copy number repeat region (68–71 Mb. There were, surprisingly, few breakpoints close to APC, contrary to expectations were there constraints on tumorigenesis caused by uncovering recessive lethal alleles or if mitotic recombination were mechanistically favoured by a longer residual chromosome arm. The locations of mitotic and meiotic recombination breakpoints were correlated, suggesting that the two types of recombination are influenced by similar processes, whether mutational or selective in origin. Breakpoints were also associated with higher local G+C content. The recombination and gain/deletion breakpoint maps on 5q were not, however, associated, perhaps owing to selective constraints on APC dosage in early colorectal tumours. Since polymorphisms within the region of frequent mitotic recombination on 5q might influence the frequency of LOH, we tested the 68–71 Mb low copy number repeat and nearby tagSNPs, but no associations with colorectal cancer risk were found. Conclusion LOH on 5q is non-random, but local factors do not greatly influence the rate of LOH at APC or explain inter differential susceptibility to colorectal tumours.

  12. Assignment of human sprouty 4 gene to chromosome segment 5q32∼33 and analysis of its pattern of expression

    Hua Liu; Jin-Zhong Chen; Shao-Hua Gu; Jian-Liang Dai; En-Pang Zhao; Lu Huang; Wang-Xiang Xu; Yi Xie; Yu-Min Mao

    2003-04-01

    The human sprouty 4 (SPYR4) gene was localized to chromosome band 5q32∼33 by screening the Stanford radiation hybrid G3 panel using a SPRY4-specific primer pair for PCR. Northern blot analysis revealed two different mRNAs (5 kb and 2 kb) in liver, skeletal muscle, heart, lung, kidney, spleen, placenta and small intestine. Reverse transcriptase-PCR analysis showed that SPYR4 was expressed in all tested tissues to different levels.

  13. The human and mouse receptors of hyaluronan-mediated motility, RHAMM, genes (HMMR) map to human chromosome 5q33.2-qter and mouse chromosome 11

    Spicer, A.P.; McDonald, J.A. [Mayo Clinic Scottsdale, AZ (United States); Roller, M.L.; Camper, S.A. [Univ. of Michigan Medical School, Ann Arbor, MI (United States)] [and others

    1995-11-01

    The gene for the receptor for hyaluronan-mediated motility, RHAAM (designated hyaluronan-mediated motility receptor, HMMR (human) and Hmmr (mouse), for mapping purposes), was localized to human chromosome 5q33.2-qter by somatic cell and radiation hybrid analyses. Investigation of two interspecific back-crosses localized the mouse RHAMM (Hmmr) locus 18 cM from the centromere of mouse chromosome 11 within a region of synteny homology with human chromosome 5q23-q35 genes. The map position of the human RHAMM gene places it in a region comparatively rich in disease-associated genes, including those for low-frequency hearing loss, dominant limb-girdle muscular dystrophy, diastrophic dysplasia, Treacher Collins syndrome, and myeloid disorders associated with the 5q-syndrome. The RHAMM gene location and its ability to transform cells when overexpressed implicate RHAMM as a possible candidate gene in the pathogenesis of the recently described t(5;14)(q33-q34;q11) acute lymphoblastic leukemias. 18 refs., 1 fig.

  14. A novel mechanism for outbursts of Comet 17P/Holmes and other short-period comets

    Miles, Richard

    2007-01-01

    A mechanism is proposed to explain the outburst of comet 17P/Holmes based on; (a) oxidation of water within the porous surface of the comet nucleus to form hydrogen peroxide (H2O2) through exposure to UV radiation, to energetic solar-wind particles and to cosmic radiation, (b) concentration of the H2O2 component through solid-, liquid- and gas-phase processes involving sublimation, evaporation, fractional crystallization, diffusion, supercooling, capillary wetting and migration in voids within the nucleus, and (c) rapid exothermic decomposition of aqueous H2O2 liberating oxygen gas via a surface catalytic reaction through interaction with finely-dispersed transition metals, metal compounds and minerals, in particular those containing Fe, localised within a differentiated multi-component comet nucleus. An accelerated release of gaseous oxygen, concomitant self-heating and volatilisation of hydrocarbons within the nucleus results in its explosive disruption. This mechanism may also explain the observation of a ...

  15. Intercombination decay of 3s3p3P10 in Mg I-like Ni and Cu

    The intercombination transition 3s21S0-3s3p 3p10 in Ni16+ and Cu17+ has been studied by beam-foil spectroscopic methods. Decay curve analysis yields lifetime values of (12.0+-1.0) ns and (8.8+-0.6) ns for Ni and Cu in agreement with various predictions. (orig./WL)

  16. Deletion 17p11.2 (Smith-Magenis syndrome) is relatively common among patients having mental retardation and myopia

    Finucane, B.; Jaeger, E.R. [Elwyn, Inc. PA (United States); Freitag, S.K. [Jefferson Medical College, Philadelphia, PA (United States)

    1994-09-01

    We recently reported the finding of moderate to severe myopia in 6 of 10 patients with Smith-Magenis syndrome (SMS). To investigate the prevalence of SMS among mentally retarded people having myopia, we surveyed a cohort of patients residing at a facility for individuals with mental retardation (MR). Of 547 institutionalized individuals with MR, 72 (13.2%) had moderate to high myopia defined as a visual acuity of minus 3 diopters or more. It should be noted that our institution does not specifically select for people with visual impairment; rather, the facility serves people with a primary diagnosis of MR. Sixty-five of 72 (90.3%) myopic individuals identified were available for cytogenetic analysis. Seventeen (26.2%) of these patients had trisomy 21. Down syndrome (DS) is well known to be associated with eye abnormalities, including myopia. Of 48 individuals with moderate to high myopia not having DS, 5 (10.4%) were shown to have deletions of 17p11.2. This is a high prevalence considering the relative rarity of SMS. By contrast, in a randomized sample of 48 patients without significant myopia at the same facility, we found no individuals with deletion 17p11.2. We conclude that the diagnosis of SMS should be considered in any non-Down syndrome individual having MR and myopia, and that ophthalmologists serving people with MR should be made aware of this deletion syndrome. Furthermore, our results suggest that significant numbers of people having SMS could be identified through selective institutional screening of patients having a combination of MR and moderate to severe myopia.

  17. Fine-scale mapping of the 5q11.2 breast cancer locus reveals at least three independent risk variants regulating MAP3K1

    Glubb, Dylan M; Maranian, Mel J; Michailidou, Kyriaki;

    2015-01-01

    Genome-wide association studies (GWASs) have revealed SNP rs889312 on 5q11.2 to be associated with breast cancer risk in women of European ancestry. In an attempt to identify the biologically relevant variants, we analyzed 909 genetic variants across 5q11.2 in 103,991 breast cancer individuals an...

  18. Transition probability values of the 1s22s3p 3P0 level in Be-like ions

    Transition probability values from the 1s22s3p 3P0 level for selected beryllium-like ions, from Z = 5 to 92, are calculated using the multi-configuration Dirac–Fock method including QED corrections, and full correlation up to the 4f subshell in both initial and final levels, for 1s22s3s 3S1, 1s22s2p 3P2 level and 1s22p23P1 decay modes of this level. (paper)

  19. Chromosome 17p13.2 transfer reverts transformation phenotypes and Fas-mediated apoptosis in breast epithelial cells.

    Lareef, Mohamed H; Tahin, Quivo; Song, Joon; Russo, Irma H; Mihaila, Dana; Slater, Carolyn M; Balsara, Binaifer; Testa, Joseph R; Broccoli, Dominique; Grobelny, Jennifer V; Mor, Gil; Cuthbert, Andrew; Russo, Jose

    2004-04-01

    Transformation of the human breast epithelial cells (HBEC) MCF-10F with the carcinogen benz(a)pyrene (BP) into BP1-E cells resulted in the loss of the chromosome 17 p13.2 locus (D17S796 marker) and formation of colonies in agar-methocel (colony efficiency (CE)), loss of ductulogenic capacity in collagen matrix, and resistance to anti-Fas monoclonal antibody (Mab)-induced apoptosis. For testing the role of that specific region of chromosome 17 in the expression of transformation phenotypes, we transferred chromosome 17 from mouse fibroblast donors to BP1-E cells. Chromosome 11 was used as negative control. After G418 selection, nine clones each were randomly selected from BP1-E-11neo and BP1-E-17neo hybrids, respectively, and tested for the presence of the donor chromosomes by fluorescent in situ hybridization and polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) analyses. Sensitivity to Fas Mab-induced apoptosis and evaluation of transformation phenotype expression were tested in MCF-10F, BP1-E, and nine BP1-E-11neo and BP1-E-17neo clones each. Six BP1-E-17neo clones exhibited a reversion of transformation phenotypes and a dose dependent sensitivity to Fas Mab-induced apoptosis, behaving similarly to MCF-10F cells. All BP1-E-11neo, and three BP1-E-17neo cell clones, like BP1-E cells, retained a high CE, loss of ductulogenic capacity, and were resistant to all Fas Mab doses tested. Genomic analysis revealed that those six BP1-E-17neo clones that were Fas-sensitive and reverted their transformed phenotypes had retained the 17p13.2 (D17S796 marker) region, whereas it was absent in all resistant clones, indicating that the expression of transformation phenotypes and the sensitivity of the cells to Fas-mediated apoptosis were under the control of genes located in this region. PMID:15057875

  20. Chromosome 3p alterations in pancreatic endocrine neoplasia

    Amato, Eliana; Barbi, Stefano; Malpeli, Giorgio; Bersani, Samantha; Pelosi, Giuseppe; Capelli, Paola

    2010-01-01

    Pancreatic endocrine tumors (PET) are rare neoplasms classified as functioning (F-PET) or non-functioning (NF-PET) according to the presence of a clinical syndrome due to hormonal hypersecretion. PETs show variable degrees of clinical aggressiveness and loss of chromosome 3p has been suggested to be associated with an advanced stage of disease. We assessed chromosome 3p copy number in 113 primary PETs and 32 metastases by fluorescence in situ hybridization (FISH) using tissue microarrays. The series included 56 well-differentiated endocrine tumors (WDET), 62 well-differentiated endocrine carcinomas (WDEC), and 6 poorly differentiated endocrine carcinomas (PDEC). Chromosome 3p alterations were found in 23/113 (20%) primary tumors, with losses being predominant over gains (14% vs. 6%). Loss of 3p was found in 5/55 (9%) WDET, 11/52 (21%) WDEC, and never in PDEC. Gains of 3p were detected in 4/55 (7%) WDET, no WDEC, but notably in 3/6 (50%) PDEC (OR 23.6; P = 0.003). Metastases were more frequently monosomic for 3p compared to primary tumors (OR 3.6; P = 0.005). Monosomy was significantly associated with larger tumor size, more advanced tumor stage, and metastasis. No association was found with survival. Chromosome 3p copy number alterations are frequent events in advanced stage PET, with gains prevailing in PDEC while losses are more frequent in WDEC, supporting the view that a specific pattern of alterations are involved in these diverse disease subtypes. PMID:20981439

  1. 人膀胱移行细胞癌染色体17p13区域杂合性缺失精细作图

    郑闪; 张建军; 等

    2002-01-01

    目的:明确染色体17p13区域等位基因杂合性缺失(LOH)在人膀胱移行细胞癌(TCC)中的发生频率和最小缺失区域,为膀胱TCC相关抑癌基因的定位克隆提供线索。方法:应用位于17p13区域的13个微卫星标记,分期的关系,结果:44例患者中,35例(79.5%)的膀胱TCC组织中存在至少1个微卫星标记的LOH,缺失频率最高的微卫星标记是位于17p13.2的D17S513,达41.4%(12/29),其次为位于17p13.3的D17S1308,为40.5%(17/42);最低为位于17p13.1的D17S261,为14.3%(4/28)LOH主要集中于三个区域;位于17p13.3的D17S695-D17S1308,位于17p13.2的D17S1533-D17S831,以及位于17p13.1区域的TP53。所检测微卫星标记中仅TP53的LOH与膀胱TCC病理分级(χ2=5.104,P<0.05),和分期(χ2=5.382,P<0.05)呈正相关,结论:在17p13区域除TP53基因以外,还可能存在两个与膀胱TCC相关的抑癌基因,分别位于17p13.3的D17S695-D17S1308和17p13.3的D17S1533-D17S831,TP53的LOH是膀胱TCC发展的晚期事件,而17p13.3和17p13.2区域的LOH则可能是膀胱TCC发生的早期事件。

  2. [17p13.3 duplication as a cause of psychomotor developmental delay in an infant - a further case of a new syndrome].

    Przybylska-Kruszewska, Amanda; Kutkowska-Kaźmierczak, Anna; Krzywdzińska, Amanda; Smyk, Marta; Nowakowska, Beata; Gryglicka, Halina; Obersztyn, Ewa; Hozyasz, Kamil K

    2016-04-29

    17p13.3 duplication is a rare and heterogeneous genetic syndrome. Microdeletions of this region are responsible for the symptoms of Miller-Dieker syndrome. We present a case of 17p13.3 duplication consisting of about 730kb in a patient with psychomotor developmental delay, concerning eye-hand coordination, posture, locomotion and speech. Among other symptoms, we found excessive physical development in relation to age, hypotonia, dysmorphic facial features (high and prominent forehead, low-set ears, hypertelorism, short nose, small upturned nose, narrow lips and pointed chin) and discrete changes in the CNS - enhanced frontal horns of the lateral ventricles and quite narrow corpus callosum. These symptoms overlap with phenotype of previously described patients with 17p13.3 duplication. The aberration has been identified by array comparative genomic hybridization (aCGH) and confirmed by fluorescence in situ hybridization (FISH). This publication presents a detailed, comparative characteristic of clinical fetures expression in discussed patient with 17p13.3 duplication and patients previously described in medical literature. Further cases with different variants of 17p13.3 duplication may contribute to characterise the specific genotypephenotype correlation. PMID:27137828

  3. Clinical and cytogenetic features of a Potocki-Lupski syndrome with the shortest 0.25Mb microduplication in 17p11.2 including RAI1.

    Lee, Cha Gon; Park, Sang-Jin; Yim, Shin-Young; Sohn, Young Bae

    2013-08-01

    Potocki-Lupski syndrome (PTLS [MIM 610883]) is a recently recognized microduplication syndrome associated with 17p11.2. It is characterized by mild facial dysmorphic features, hypermetropia, infantile hypotonia, failure to thrive, mental retardation, autistic spectrum disorders, behavioral abnormalities, sleep apnea, and cardiovascular anomalies. In several studies, the critical PTLS region was deduced to be 1.3Mb in length, and included RAI1 and 17 other genes. We report a 3-year-old Korean boy with the smallest duplication in 17p11.2 and a milder phenotype. He had no family history of neurologic disease or developmental delay and no history of seizure, autistic features, or behavior problems. He showed subtle facial dysmorphic features (dolichocephaly and a mildly asymmetric smile) and flat feet. All laboratory tests were normal and he had no evidence of internal organ anomalies. He was found to have mild intellectual disabilities (full scale IQ 65 on K-WPPSI) and language developmental delay (age of 2.2year-old on PRESS). Array comparative genomic hybridization (CGH) showed about a 0.25Mb microduplication on chromosome 17p11.2 containing four Refseq (NCBI reference sequence) genes, including RAI1 [arr 17p11.2(17,575,978-17,824,623)×3]. When compared with previously reported cases, the milder phenotype of our patient may be associated with the smallest duplication in 17p11.2, 0.25Mb in length. PMID:23078968

  4. Large Excess of Heavy Nitrogen in Both Hydrogen Cyanide and Cyanogen from Comet 17P/Holmes

    Bockelée-Morvan, D.; Biver, N.; Jehin, E.; Cochran, A. L.; Wiesemeyer, H.; Manfroid, J.; Hutsemékers, D.; Arpigny, C.; Boissier, J.; Cochran, W.; Colom, P.; Crovisier, J.; Milutinovic, N.; Moreno, R.; Prochaska, J. X.; Ramirez, I.; Schulz, R.; Zucconi, J.-M.

    2008-05-01

    From millimeter and optical observations of the Jupiter-family comet 17P/Holmes performed soon after its huge outburst of 2007 October 24, we derive 14N/15N = 139 ± 26 in HCN and 14N/15N = 165 ± 40 in CN, establishing that HCN has the same nonterrestrial isotopic composition as CN. The same conclusion is obtained for the long-period comet C/1995 O1 (Hale-Bopp) after a reanalysis of previously published measurements. These results are compatible with HCN being the prime parent of CN in cometary atmospheres. The15N excess relative to the Earth's atmospheric value indicates that N-bearing volatiles in the solar nebula underwent important N isotopic fractionation at some stage of solar system formation. HCN molecules never isotopically equilibrated with the main nitrogen reservoir in the solar nebula before being incorporated in Oort Cloud and Kuiper Belt comets. The 12C/13C ratios in HCN and CN are measured to be consistent with the terrestrial value.

  5. Familial reciprocal translocation t(8;17(p23;q21 in a woman with recurrent spontaneous abortion

    Shadi F. Al-Ashi

    2013-08-01

    Full Text Available This work presents the results of cytogenetic analysis of a couple referred to our genetics laboratory with ten first trimester abortions and one IVF failure. The male showed a normal (46, XY karyotype whereas the female was found to carry an apparently balanced reciprocal translocation [46, XX, t(8;17(p23;q21]. Two sisters and two brothers of the eight siblings of the female proved to have the same translocation. Although the female\\'s father is deceased and his sample was not available for investigation. The origin of this translocation must be paternal since the female\\'s mother harbored a normal karyotype. It is concluded that the history of recurrent pregnancy losses in the couple is due to the production of unbalanced gametes in the female as a result of the reciprocal translocation she has and the couple was advised to undergo a PGD for embryo selection prior to their future IVF trials. The authors also recommend that all RSA couples with normal routine work-up results should be offered chromosomal analysis without delay. [Int J Reprod Contracept Obstet Gynecol 2013; 2(4.000: 695-697

  6. The promoter analysis of the human C17orf25 gene, a novel chromosome 17p13.3 gene

    JIAN; YING; GUO; JIAN; XU; DA; QIN; MAO; LI; LI; FU; JIAN; REN; GU; JING; DE; ZHU

    2002-01-01

    The human C17orf25 gene (Accession No. AF177342) is one of thirteen genes cloned from a regiondisplaying a high score of loss of heterozygosity within chromosome 17p13.3 in human hepatocellular car-cinoma in China[1]. To unveil the underlying mechanisms for the transcription regulation of this gene andunderstand its implication to the hepatocellular carcinogenesis, we looked into the relevant aspects by bothbioinformatic and experimental executions. We found: 1, The abundant expression of the C17orf25 genewas evident in all the cell lines and tissue samples tested, showing little hepatoma-selectivity; 2, Its tran-scription starts at a single site, locating at -60 from the translation initiation codon; 3, A 58 bp fragmentcontaining the transcription start, extending from -112 to -55, represents the minimal promoter; 4, Theconsensus sequence within this fragment recognized by SP1 contributes predominantly to the activity of theminimal promoter; 5, The bioinformatic analysis suggests that the C17orf25 gene may encode a protein inthe family of the glyoxalase. Our data has provided some deep insight into both function and regulation ofthe C17orf25 gene in the context of the normal liver and hepatocellular carcinoma.

  7. Size-tunable, hexagonal plate-like Cu3P and Janus-like Cu-Cu3P nanocrystals.

    De Trizio, Luca; Figuerola, Albert; Manna, Liberato; Genovese, Alessandro; George, Chandramohan; Brescia, Rosaria; Saghi, Zineb; Simonutti, Roberto; Van Huis, Marijn; Falqui, Andrea

    2012-01-24

    We describe two synthesis approaches to colloidal Cu(3)P nanocrystals using trioctylphosphine (TOP) as phosphorus precursor. One approach is based on the homogeneous nucleation of small Cu(3)P nanocrystals with hexagonal plate-like morphology and with sizes that can be tuned from 5 to 50 nm depending on the reaction time. In the other approach, metallic Cu nanocrystals are nucleated first and then they are progressively phosphorized to Cu(3)P. In this case, intermediate Janus-like dimeric nanoparticles can be isolated, which are made of two domains of different materials, Cu and Cu(3)P, sharing a flat epitaxial interface. The Janus-like nanoparticles can be transformed back to single-crystalline copper particles if they are annealed at high temperature under high vacuum conditions, which makes them an interesting source of phosphorus. The features of the Cu-Cu(3)P Janus-like nanoparticles are compared with those of the striped microstructure discovered more than two decades ago in the rapidly quenched Cu-Cu(3)P eutectic of the Cu-P alloy, suggesting that other alloy/eutectic systems that display similar behavior might give origin to nanostructures with flat, epitaxial interface between domains of two diverse materials. Finally, the electrochemical properties of the copper phosphide plates are studied, and they are found to be capable of undergoing lithiation/delithiation through a displacement reaction, while the Janus-like Cu-Cu(3)P particles do not display an electrochemical behavior that would make them suitable for applications in batteries. PMID:22136519

  8. Au Nanowire-Striped Cu3P Platelet Photoelectrocatalysts.

    Dutta, Anirban; Samantara, Aneeya K; Adhikari, Samrat Das; Jena, Bikash Kumar; Pradhan, Narayan

    2016-03-17

    A stripy pattern of continuous epitaxial growth of thin Au nanowires on plasmonic Cu3P platelets is reported. The obtained Au-Cu3P heterostructures retain their wide area interfacial heterojunction, which is typically not observed in metal-semiconductor heterostructures. This is performed by phosphine-mediated in situ reduction of Au ions on specific facets of Cu3P platelets. The intriguing stripy movements of nanowires are regulated by strong surface binding ligands. Because this is a dual plasmon heterostructure with wide visible absorption window, these are further explored as a photoelectrocatalyst for efficient hole transfer and sensing of an important biomolecule, nicotinamide adenine dinucleotide (NADH). The observed anodic photocurrent was 30 times higher in the presence of NADH, and this proves that the heterostructured material is an ideal photosenser and an efficient catalyst for solar energy conversion. PMID:26938025

  9. 5q14.3 deletion neurocutaneous syndrome: Contiguous gene syndrome caused by simultaneous deletion of RASA1 and MEF2C: A progressive disease.

    Ilari, Rita; Agosta, Guillermo; Bacino, Carlos

    2016-03-01

    We report the case of a young girl who was presented with complex clinical symptoms caused by the deletion of contiguous genes: RASA1 and MEF2C, located on chromosome 5q14.3. Specifically, the diagnosis of her skin disorder and vascular malformations involving central nervous system is consistent with a RASopathy. The child's neurological manifestations are observed in most patients suffering from 5q14.3 by deletion or mutation of the MEF2C gene. A review of the literature allowed us to conclude that the contiguous deletion of genes RASA1 and MEF2C fulfills the criteria for the diagnosis of a Neurocutaneous syndrome as proposed by Carr et al. [2011]. We also assessed the penetrance of RASA1 and clinical manifestations of MEF2C according to the type of deletion. This child described presents the complete symptomatology of both deleted genes. We would also like to highlight the progression of the disorder. PMID:26774077

  10. Identification of a Potential Regulatory Variant for Colorectal Cancer Risk Mapping to Chromosome 5q31.1: A Post-GWAS Study

    Ke, Juntao; Lou, Jiao; Chen, Xueqin; Li, Jiaoyuan; Liu, Cheng; Gong, Yajie; Yang, Yang; Zhu, Ying; Zhang, Yi; Gong, Jing

    2015-01-01

    Large-scale genome-wide association studies (GWAS) have established chromosome 5q31.1 as a susceptibility locus for colorectal cancer (CRC), which was still lack of causal genetic variants. We searched potentially regulatory single nucleotide polymorphisms (SNPs) in the overlap region between linkage disequilibrium (LD) block of 5q31.1 and regulatory elements predicted by histone modifications, then tested their association with CRC via a case-control study. Among three candidate common variants, we found rs17716310 conferred significantly (heterozygous model: OR = 1.273, 95% confidence interval (95%CI) = 1.016–1.595, P = 0.036) and marginally (dominant model: OR = 1.238, 95%CI = 1.000–1.532, P = 0.050) increase risk for CRC in a Chinese population including 695 cases and 709 controls. This variation was suggested to be regulatory altering the activity of enhancer that control PITX1 expression. Using epigenetic information such as chromatin immunoprecipitation-sequencing (ChIP-seq) data might help researchers to interpret the results of GWAS and locate causal variants for diseases in post-GWAS era. PMID:26381143

  11. Dosage changes of a segment at 17p13.1 lead to intellectual disability and microcephaly as a result of complex genetic interaction of multiple genes

    Carvalho, Claudia M B; Vasanth, Shivakumar; Shinawi, Marwan;

    2014-01-01

    The 17p13.1 microdeletion syndrome is a recently described genomic disorder with a core clinical phenotype of intellectual disability, poor to absent speech, dysmorphic features, and a constellation of more variable clinical features, most prominently microcephaly. We identified five subjects...... with copy-number variants (CNVs) on 17p13.1 for whom we performed detailed clinical and molecular studies. Breakpoint mapping and retrospective analysis of published cases refined the smallest region of overlap (SRO) for microcephaly to a genomic interval containing nine genes. Dissection of this phenotype...... of discrete gene pairings induce microcephaly. Taken together, these studies support a model in which concomitant dosage perturbation of multiple genes within the CNV drive the microcephaly and possibly other neurodevelopmental phenotypes associated with rearrangements in the 17p13.1 SRO....

  12. P3P semantic checker of site behaviours

    Grande, Robson Eduardo; Zorzo, Sérgio Donizetti

    2006-01-01

    The interactive use of the web between users and service providers introduces a privacy problem that involves the undesired disclosing of user personal information, mainly with the presence of personalization that needs this type of information. Also there are many manners to face it, but the Platform for Privacy Preferences (P3P) is one that provides a variable level of privacy for the user’s browsing. However, the P3P only introduces a privacy contract between the site and the user, without...

  13. Dsj meson decay in the C3P0 model

    Full text. Fock-Tani is a field theory formalism appropriated for the simultaneous treatment of composite particles and their constituents. The formalism was originally developed for the treatment of problems in atomic physics and it was extended later on to the treatment of problems on hadron physics. In the Fock-Tani formalism one starts with the Fock representation of the system using field operators of elementary constituents which satisfy canonical (anti) commutation relations. Composite-particle field operators are linear combinations of the elementary-particle operators and do not generally satisfy canonical (anti) commutation relations. 'Ideal' field operators acting on an enlarged Fock space are then introduced in close correspondence with the composite ones. Next, a given unitary transformation, which transforms the single composite states into single ideal states, is introduced. Application of the unitary operator on the microscopic Hamiltonian, or on other hermitian operators expressed in terms of the elementary constituent field operators, gives equivalent operators which contain the ideal field operators. The effective Hamiltonian in the new representation has a clear physical interpretation in terms of the processes it describes. Since all field operators in the new representation satisfy canonical (anti)commutation relations, the standard methods of quantum field theory can then be readily applied. For a long time the pair creation models for strong hadronic decays have been formulated. The 3P0 model is typical decay model which considers only OZI-allowed strong decays. The 3P0 model considers a quark-antiquark par creation in the presence of the initial state meson. The quark-antiquark par is created with the vacuum quantum numbers. This model can also be obtained from the non-relativistic limit of the pair creation Hamiltonian. Applying the Fock-Tani transformation to the pair creation Hamiltonian produces the characteristic expansion in powers of

  14. Myelodysplastic disorders carrying both isolated del(5q and JAK2V617F mutation: concise review, with focus on lenalidomide therapy

    Musto P

    2014-06-01

    Full Text Available Pellegrino Musto,1 Vittorio Simeon,2 Roberto Guariglia,3 Gabriella Bianchino,4 Vitina Grieco,4 Filomena Nozza,4 Francesco La Rocca,2 Gioacchino Marziano,1 Anna Vittoria Lalinga,5 Emiliano Fabiani,6 Maria Teresa Voso,6 Patrizia Scaravaglio,7 Cristina Mecucci,8 Giovanni D'Arena31Scientific Direction, 2Laboratory of Preclinical and Translational Research, 3Unit of Hematology and Stem Cell Transplantation, 4Laboratory of Clinical Research and Advanced Diagnostics, 5Pathology Unit, IRCCS, Centro di Riferimento Oncologico della Basilicata, Rionero in Vulture, Italy; 6Department of Hematology, Universita Cattolica del Sacro Cuore, Rome, Italy; 7Laboratory of Internal Medicine and Hematology, S Luigi Gonzaga Hospital, Orbassano, Italy; 8Hematology and Bone Marrow Transplantation Unit, University of Perugia, Perugia, ItalyAbstract: The concomitant presence of del(5q and JAK2V617F mutation is an infrequent event which occurs in rare patients with peculiar cytogenetic, molecular, morphological and clinical features, resembling those of both myelodysplastic syndromes and myeloproliferative neoplasms. Lenalidomide may induce rapid, profound, and long-lasting responses in a subset of these patients. However, the mechanism(s by which the drug acts in these conditions remain not completely elucidated. A new case report and a review of all cases published so far in this setting are provided. Furthermore, the possibility of categorizing – from a clinical, pathological, and biological point of view – for at least some of these patients as a potential distinct entity is discussed.Keywords: myelodysplastic syndromes, myeloproliferative neoplasms, lenalidomide, del(5q, JAK2, World Health Organization

  15. Lenalidomide for the Treatment of Low- or Intermediate-1-Risk Myelodysplastic Syndromes Associated with Deletion 5q Cytogenetic Abnormality: An Evidence Review of the NICE Submission from Celgene.

    Blommestein, Hedwig M; Armstrong, Nigel; Ryder, Steve; Deshpande, Sohan; Worthy, Gill; Noake, Caro; Riemsma, Rob; Kleijnen, Jos; Severens, Johan L; Al, Maiwenn J

    2016-01-01

    The National Institute for Health and Care Excellence (NICE) invited the manufacturer of lenalidomide (Celgene) to submit evidence of the clinical and cost effectiveness of the drug for treating adults with myelodysplastic syndromes (MDS) associated with deletion 5q cytogenetic abnormality, as part of the Institute's single technology appraisal (STA) process. Kleijnen Systematic Reviews Ltd (KSR), in collaboration with Erasmus University Rotterdam, was commissioned to act as the Evidence Review Group (ERG). This paper describes the company's submission, the ERG review, and the NICE's subsequent decisions. The ERG reviewed the evidence for clinical and cost effectiveness of the technology, as submitted by the manufacturer to the NICE. The ERG searched for relevant additional evidence and validated the manufacturer's decision analytic model to examine the robustness of the cost-effectiveness results. Clinical effectiveness was obtained from a three-arm, European, randomized, phase III trial among red blood cell (RBC) transfusion-dependent patients with low-/intermediate-1-risk del5q31 MDS. The primary endpoint was RBC independence for ≥26 weeks, and was reached by a higher proportion of patients in the lenalidomide 10 and 5 mg groups compared with placebo (56.1 and 42.6 vs 5.9 %, respectively; both p submitted a Patient Access Scheme (PAS) that provided lenalidomide free of charge for patients who remained on treatment after 26 cycles. This PAS improved the ICER to £25,300, although the AC considered the proportion of patients who received treatment beyond 26 cycles, and hence the ICER, to be uncertain. Nevertheless, the AC accepted a commitment from the manufacturer to publish, once available, data on the proportion of patients eligible for the PAS, and believed this provided reassurance that lenalidomide was a cost-effective treatment for low- or intermediate-1-risk MDS patients. PMID:26314282

  16. Doubly excited 3P(e) resonant states in Ps(-)

    Ho, Y. K.; Bhatia, A. K.

    1992-01-01

    Doubly excited 3P(e) resonant states in Ps(-) are calculated using a method of complex-coordinate rotation. Resonance parameters (both resonance positions and widths) for doubly excited states associated with the n = 2, 3, 4, 5, and 6 thresholds of positronium atoms are evaluated using elaborate Hylleraas-type functions. In addition to ten Feshbach-type resonances lying below various Ps thresholds, three shape resonances were identified, one each lying above the n = 2, 4, and 6 Ps thresholds. It is further noted that the energy levels for the present 3P(e) states are nearly degenerate with respect to the previously calculated 1P(0) states. Such a symmetric character suggests that the highly and doubly excited Ps(-), similar to its counterpart in H(-), would exhibit rovibrational behaviors analogous to those of XYX triatomic molecules.

  17. Configuration interaction calculations of positron binding to Be(3P )

    The configuration interaction method is applied to investigate the possibility of positron binding to the metastable beryllium (1s22s2p 3P ) state. The largest calculation obtained an estimated energy that was unstable by 0.00014 Hartree with respect to the Ps + Be+(2s) lowest dissociation channel. It is likely that positron binding to parent states with non-zero angular momentum is inhibited by centrifugal barriers

  18. Spatial and electronic structure of the Ni3P surface

    To understand the catalytic effect in the Ni-Ni3P for the growth of carbon nanostructures, the structural and electronic properties of Ni3P surface are calculated from first-principles calculations. The calculated surface energies for the (0 0 1)-Ni4P4-terminated surface, the (0 0 1)-Ni8-terminated surface, and the (1 1 0)-Ni8-terminated surface show that the (0 0 1)-Ni4P4-terminated surface is energetically more stable within the allowed range of the chemical potential of P. Through the analysis of the partial density of states of Ni and P atoms in surface and bulk states, respectively, it is further found that due to the bond contractions of the surface layer, the core-level shifts of P atoms in the (0 0 1)-Ni4P4-terminated surface make P atoms in the Ni3P particles act as a catalyst. Finally, the obtained results of the work function show that the (0 0 1)-Ni4P4-terminated surface has the largest work function when compared with the other two studied surfaces.

  19. JS3P: junior staff programme pilot project

    Pretrel, H.; Tregoures, N.; Bessiron, V.; Dehoyos, A.; Delvallee, I.; Brisson, N.; Debayle, C.; Dubreuil, M.; Nicaise, G.; Perignon, J.P.; Richard, J. [Institut de Radioprotection et de Surete Nucleaire (IRSN), 13 - Saint-Paul-lez-Durance (France); Reinke, N.; Kaulard, J.; Burgener, M.; Keesmann, S.; Schramm, B.; Seubert, A.; Sternkopf, J.; Thuma, G.; Weber, S. [Gesellschaft fuer Anlagen- und Reaktorsicherheit mbH (GRS), Koln (Germany); Smidts, O.; Maillet, E.; Bucalossi, A.; Van haesendonck, M.; Uyttenhove, W.; Mertens, J. [AVN, Bruxelles (Belgium)

    2006-07-01

    Concept: The objective of the project is to allow junior staff members from the European Technical Safety Organisations (TSOs), IRSN, GRS and AVN, to work together with the final goal of creating a junior staff network, based on technical, cultural and personal interests. These projects are to show junior staff members at a very early stage during their career the need for European collaborations. They are also a tool to explore new subjects of co-operation. It is an initiative that should strengthen the links between the organisations and contribute to establishing the future of nuclear safety in Europe. A JS3P (Junior Staff Programme Pilot Project) is a project done jointly by 'junior' staff members from the three TSOs, where experience of 'seniors' is also integrated when needed. Compared to other collaborative activities, it has certain specific features. The JS3P favours staff exchanges, and technical meetings of several days should be planned during a project in order to encourage people to work together. Technical objectives are shared and the work is done jointly (reports, articles). The team involved in the JS3P should be as small as possible to favour its efficiency. The JS3P is short and easy to realize. Its duration is fixed to a maximum of about 12 months with the option to be prolonged. Typical topics are bibliographic work, comparison issues, scientific surveys, benchmark exercises and prospective investigations on innovative ideas. They can be linked to existing joint projects and then form a smaller module integrated into the large project. Topics may concern prospective issues, tentatively investigating new topics that can be seen as exploratory co-operation projects. Subjects may also concern research issues that are not a priority but that deserve to be investigated as new attractive topics. The JS3P is defined and managed by junior staff members. It is approved by a management board committee and supervised by a technical

  20. JS3P: junior staff programme pilot project

    Concept: The objective of the project is to allow junior staff members from the European Technical Safety Organisations (TSOs), IRSN, GRS and AVN, to work together with the final goal of creating a junior staff network, based on technical, cultural and personal interests. These projects are to show junior staff members at a very early stage during their career the need for European collaborations. They are also a tool to explore new subjects of co-operation. It is an initiative that should strengthen the links between the organisations and contribute to establishing the future of nuclear safety in Europe. A JS3P (Junior Staff Programme Pilot Project) is a project done jointly by 'junior' staff members from the three TSOs, where experience of 'seniors' is also integrated when needed. Compared to other collaborative activities, it has certain specific features. The JS3P favours staff exchanges, and technical meetings of several days should be planned during a project in order to encourage people to work together. Technical objectives are shared and the work is done jointly (reports, articles). The team involved in the JS3P should be as small as possible to favour its efficiency. The JS3P is short and easy to realize. Its duration is fixed to a maximum of about 12 months with the option to be prolonged. Typical topics are bibliographic work, comparison issues, scientific surveys, benchmark exercises and prospective investigations on innovative ideas. They can be linked to existing joint projects and then form a smaller module integrated into the large project. Topics may concern prospective issues, tentatively investigating new topics that can be seen as exploratory co-operation projects. Subjects may also concern research issues that are not a priority but that deserve to be investigated as new attractive topics. The JS3P is defined and managed by junior staff members. It is approved by a management board committee and supervised by a technical steering committee

  1. Fine-Scale Mapping of the 5q11.2 Breast Cancer Locus Reveals at Least Three Independent Risk Variants Regulating MAP3K1

    Glubb, Dylan M.; Maranian, Mel J.; Michailidou, Kyriaki; Pooley, Karen A.; Meyer, Kerstin B.; Kar, Siddhartha; Carlebur, Saskia; O’Reilly, Martin; Betts, Joshua A.; Hillman, Kristine M.; Kaufmann, Susanne; Beesley, Jonathan; Canisius, Sander; Hopper, John L.; Southey, Melissa C.; Tsimiklis, Helen; Apicella, Carmel; Schmidt, Marjanka K.; Broeks, Annegien; Hogervorst, Frans B.; van der Schoot, C. Ellen; Muir, Kenneth; Lophatananon, Artitaya; Stewart-Brown, Sarah; Siriwanarangsan, Pornthep; Fasching, Peter A.; Ruebner, Matthias; Ekici, Arif B.; Beckmann, Matthias W.; Peto, Julian; dos-Santos-Silva, Isabel; Fletcher, Olivia; Johnson, Nichola; Pharoah, Paul D.P.; Bolla, Manjeet K.; Wang, Qin; Dennis, Joe; Sawyer, Elinor J.; Tomlinson, Ian; Kerin, Michael J.; Miller, Nicola; Burwinkel, Barbara; Marme, Frederik; Yang, Rongxi; Surowy, Harald; Guénel, Pascal; Truong, Thérèse; Menegaux, Florence; Sanchez, Marie; Bojesen, Stig E.; Nordestgaard, Børge G.; Nielsen, Sune F.; Flyger, Henrik; González-Neira, Anna; Benitez, Javier; Zamora, M. Pilar; Arias Perez, Jose Ignacio; Anton-Culver, Hoda; Neuhausen, Susan L.; Brenner, Hermann; Dieffenbach, Aida Karina; Arndt, Volker; Stegmaier, Christa; Meindl, Alfons; Schmutzler, Rita K.; Brauch, Hiltrud; Ko, Yon-Dschun; Brüning, Thomas; Nevanlinna, Heli; Muranen, Taru A.; Aittomäki, Kristiina; Blomqvist, Carl; Matsuo, Keitaro; Ito, Hidemi; Iwata, Hiroji; Tanaka, Hideo; Dörk, Thilo; Bogdanova, Natalia V.; Helbig, Sonja; Lindblom, Annika; Margolin, Sara; Mannermaa, Arto; Kataja, Vesa; Kosma, Veli-Matti; Hartikainen, Jaana M.; Wu, Anna H.; Tseng, Chiu-chen; Van Den Berg, David; Stram, Daniel O.; Lambrechts, Diether; Zhao, Hui; Weltens, Caroline; van Limbergen, Erik; Chang-Claude, Jenny; Flesch-Janys, Dieter; Rudolph, Anja; Seibold, Petra; Radice, Paolo; Peterlongo, Paolo; Barile, Monica; Capra, Fabio; Couch, Fergus J.; Olson, Janet E.; Hallberg, Emily; Vachon, Celine; Giles, Graham G.; Milne, Roger L.; McLean, Catriona; Haiman, Christopher A.; Henderson, Brian E.; Schumacher, Fredrick; Le Marchand, Loic; Simard, Jacques; Goldberg, Mark S.; Labrèche, France; Dumont, Martine; Teo, Soo Hwang; Yip, Cheng Har; See, Mee-Hoong; Cornes, Belinda; Cheng, Ching-Yu; Ikram, M. Kamran; Kristensen, Vessela; Zheng, Wei; Halverson, Sandra L.; Shrubsole, Martha; Long, Jirong; Winqvist, Robert; Pylkäs, Katri; Jukkola-Vuorinen, Arja; Kauppila, Saila; Andrulis, Irene L.; Knight, Julia A.; Glendon, Gord; Tchatchou, Sandrine; Devilee, Peter; Tollenaar, Robert A.E.M.; Seynaeve, Caroline; Van Asperen, Christi J.; García-Closas, Montserrat; Figueroa, Jonine; Chanock, Stephen J.; Lissowska, Jolanta; Czene, Kamila; Klevebring, Daniel; Darabi, Hatef; Eriksson, Mikael; Hooning, Maartje J.; Hollestelle, Antoinette; Martens, John W.M.; Collée, J. Margriet; Hall, Per; Li, Jingmei; Humphreys, Keith; Shu, Xiao-Ou; Lu, Wei; Gao, Yu-Tang; Cai, Hui; Cox, Angela; Cross, Simon S.; Reed, Malcolm W.R.; Blot, William; Signorello, Lisa B.; Cai, Qiuyin; Shah, Mitul; Ghoussaini, Maya; Kang, Daehee; Choi, Ji-Yeob; Park, Sue K.; Noh, Dong-Young; Hartman, Mikael; Miao, Hui; Lim, Wei Yen; Tang, Anthony; Hamann, Ute; Torres, Diana; Jakubowska, Anna; Lubinski, Jan; Jaworska, Katarzyna; Durda, Katarzyna; Sangrajrang, Suleeporn; Gaborieau, Valerie; Brennan, Paul; McKay, James; Olswold, Curtis; Slager, Susan; Toland, Amanda E.; Yannoukakos, Drakoulis; Shen, Chen-Yang; Wu, Pei-Ei; Yu, Jyh-Cherng; Hou, Ming-Feng; Swerdlow, Anthony; Ashworth, Alan; Orr, Nick; Jones, Michael; Pita, Guillermo; Alonso, M. Rosario; Álvarez, Nuria; Herrero, Daniel; Tessier, Daniel C.; Vincent, Daniel; Bacot, Francois; Luccarini, Craig; Baynes, Caroline; Ahmed, Shahana; Healey, Catherine S.; Brown, Melissa A.; Ponder, Bruce A.J.; Chenevix-Trench, Georgia; Thompson, Deborah J.; Edwards, Stacey L.; Easton, Douglas F.; Dunning, Alison M.; French, Juliet D.

    2015-01-01

    Genome-wide association studies (GWASs) have revealed SNP rs889312 on 5q11.2 to be associated with breast cancer risk in women of European ancestry. In an attempt to identify the biologically relevant variants, we analyzed 909 genetic variants across 5q11.2 in 103,991 breast cancer individuals and control individuals from 52 studies in the Breast Cancer Association Consortium. Multiple logistic regression analyses identified three independent risk signals: the strongest associations were with 15 correlated variants (iCHAV1), where the minor allele of the best candidate, rs62355902, associated with significantly increased risks of both estrogen-receptor-positive (ER+: odds ratio [OR] = 1.24, 95% confidence interval [CI] = 1.21–1.27, ptrend = 5.7 × 10−44) and estrogen-receptor-negative (ER−: OR = 1.10, 95% CI = 1.05–1.15, ptrend = 3.0 × 10−4) tumors. After adjustment for rs62355902, we found evidence of association of a further 173 variants (iCHAV2) containing three subsets with a range of effects (the strongest was rs113317823 [pcond = 1.61 × 10−5]) and five variants composing iCHAV3 (lead rs11949391; ER+: OR = 0.90, 95% CI = 0.87–0.93, pcond = 1.4 × 10−4). Twenty-six percent of the prioritized candidate variants coincided with four putative regulatory elements that interact with the MAP3K1 promoter through chromatin looping and affect MAP3K1 promoter activity. Functional analysis indicated that the cancer risk alleles of four candidates (rs74345699 and rs62355900 [iCHAV1], rs16886397 [iCHAV2a], and rs17432750 [iCHAV3]) increased MAP3K1 transcriptional activity. Chromatin immunoprecipitation analysis revealed diminished GATA3 binding to the minor (cancer-protective) allele of rs17432750, indicating a mechanism for its action. We propose that the cancer risk alleles act to increase MAP3K1 expression in vivo and might promote breast cancer cell survival. PMID:25529635

  2. Products of the Benzene + O(3P) Reaction

    Taatjes, Craig A.; Osborn, David L.; Selby, Talitha M.; Meloni, Giovanni; Trevitt, Adam J.; Epifanovsky, Evgeny; Krylov, Anna I.; Sirjean, Baptiste; Dames, Enoch; Wang, Hai

    2009-12-21

    The gas-phase reaction of benzene with O(3P) is of considerable interest for modeling of aromatic oxidation, and also because there exist fundamental questions concerning the prominence of intersystem crossing in the reaction. While its overall rate constant has been studied extensively, there are still significant uncertainties in the product distribution. The reaction proceeds mainly through the addition of the O atom to benzene, forming an initial triplet diradical adduct, which can either dissociate to form the phenoxy radical and H atom, or undergo intersystem crossing onto a singlet surface, followed by a multiplicity of internal isomerizations, leading to several possible reaction products. In this work, we examined the product branching ratios of the reaction between benzene and O(3P) over the temperature range of 300 to 1000 K and pressure range of 1 to 10 Torr. The reactions were initiated by pulsed-laser photolysis of NO2 in the presence of benzene and helium buffer in a slow-flow reactor, and reaction products were identified by using the multiplexed chemical kinetics photoionization mass spectrometer operating at the Advanced Light Source (ALS) of Lawrence Berkeley National Laboratory. Phenol and phenoxy radical were detected and quantified. Cyclopentadiene and cyclopentadienyl radical were directly identified for the first time. Finally, ab initio calculations and master equation/RRKM modeling were used to reproduce the experimental branching ratios, yielding pressure-dependent rate expressions for the reaction channels, including phenoxy + H, phenol, cyclopentadiene + CO, which are proposed for kinetic modeling of benzene oxidation.

  3. Photoinduced Kondo effect in CeZn3P3

    Kitagawa, J.; Kitajima, D.; Shimokawa, K.; Takaki, H.

    2016-01-01

    The Kondo effect, which originates from the screening of a localized magnetic moment by a spin-spin interaction, is widely observed in nonartificial magnetic materials, artificial quantum dots, and carbon nanotubes. In devices based on quantum dots or carbon nanotubes that target quantum information applications, the Kondo effect can be tuned by a gate voltage, a magnetic field, or light. However, the manipulation of the Kondo effect in nonartificial materials has not been thoroughly studied; in particular, the artificial creation of the Kondo effect remains unexplored. Per this subject study, however, a route for the optical creation of the Kondo effect in the nonartificial material p -type semiconductor CeZn3P3 is presented. The Kondo effect emerges under visible-light illumination of the material by a continuous-wave laser diode and is ultimately revealed by photoinduced electrical resistivity, which clearly exhibits a logarithmic temperature dependency. By contrast, a La-based compound (LaZn3P3 ) displays only normal metallic behavior under similar illumination. The photoinduced Kondo effect, which occurs at higher temperatures when compared with the Kondo effect in artificial systems, provides a potential range of operation for not only quantum information/computation devices but also for operation of magneto-optic devices, thereby expanding the range of device applications based on the Kondo effect.

  4. Identification of a rare 17p13.3 duplication including the BHLHA9 and YWHAE genes in a family with developmental delay and behavioural problems

    Capra Valeria

    2012-10-01

    Full Text Available Abstract Background Deletions and duplications of the PAFAH1B1 and YWHAE genes in 17p13.3 are associated with different clinical phenotypes. In particular, deletion of PAFAH1B1 causes isolated lissencephaly while deletions involving both PAFAH1B1 and YWHAE cause Miller-Dieker syndrome. Isolated duplications of PAFAH1B1 have been associated with mild developmental delay and hypotonia, while isolated duplications of YWHAE have been associated with autism. In particular, different dysmorphic features associated with PAFAH1B1 or YWHAE duplication have suggested the need to classify the patient clinical features in two groups according to which gene is involved in the chromosomal duplication. Methods We analyze the proband and his family by classical cytogenetic and array-CGH analyses. The putative rearrangement was confirmed by fluorescence in situ hybridization. Results We have identified a family segregating a 17p13.3 duplication extending 329.5 kilobases by FISH and array-CGH involving the YWHAE gene, but not PAFAH1B1, affected by a mild dysmorphic phenotype with associated autism and mental retardation. We propose that BHLHA9, YWHAE, and CRK genes contribute to the phenotype of our patient. The small chromosomal duplication was inherited from his mother who was affected by a bipolar and borderline disorder and was alcohol addicted. Conclusions We report an additional familial case of small 17p13.3 chromosomal duplication including only BHLHA9, YWHAE, and CRK genes. Our observation and further cases with similar microduplications are expected to be diagnosed, and will help better characterise the clinical spectrum of phenotypes associated with 17p13.3 microduplications.

  5. First Detection of [C I] $^3$P$_1$-$^3$P$_0$ Emission from a Protoplanetary Disk

    Tsukagoshi, Takashi; Saito, Masao; Kitamura, Yoshimi; Shimajiri, Yoshito; Kawabe, Ryohei

    2015-01-01

    We performed single point [C I] $^3$P$_1$-$^3$P$_0$ and CO J=4-3 observations toward three T Tauri stars, DM Tau, LkCa 15, and TW Hya, using the Atacama Large Millimeter/submillimeter Array (ALMA) Band 8 qualification model receiver installed on the Atacama Submillimeter Telescope Experiment (ASTE). Two protostars in the Taurus L1551 region, L1551 IRS 5 and HL Tau, were also observed. We successfully detected [C I] emission from the protoplanetary disk around DM Tau as well as the protostellar targets. The spectral profile of the [C I] emission from the protoplanetary disk is marginally single-peaked, suggesting that atomic carbon (C) extends toward the outermost disk. The detected [C I] emission is optically thin and the column densities of C are estimated to be <~10$^{16}$ cm$^{-2}$ and ~10$^{17}$ cm$^{-2}$ for the T Tauri star targets and the protostars, respectively. We found a clear difference in the total mass ratio of C to dust, $M$(C)/$M$(dust), between the T Tauri stars and protostellar targets; t...

  6. Ge0.83Sn0.17 p-channel metal-oxide-semiconductor field-effect transistors: Impact of sulfur passivation on gate stack quality

    The effect of room temperature sulfur passivation of the surface of Ge0.83Sn0.17 prior to high-k dielectric (HfO2) deposition is investigated. X-ray photoelectron spectroscopy (XPS) was used to examine the chemical bonding at the interface of HfO2 and Ge0.83Sn0.17. Sulfur passivation is found to be effective in suppressing the formation of both Ge oxides and Sn oxides. A comparison of XPS results for sulfur-passivated and non-passivated Ge0.83Sn0.17 samples shows that sulfur passivation of the GeSn surface could also suppress the surface segregation of Sn atoms. In addition, sulfur passivation reduces the interface trap density Dit at the high-k dielectric/Ge0.83Sn0.17 interface from the valence band edge to the midgap of Ge0.83Sn0.17, as compared with a non-passivated control. The impact of the improved Dit is demonstrated in Ge0.83Sn0.17 p-channel metal-oxide-semiconductor field-effect transistors (p-MOSFETs). Ge0.83Sn0.17 p-MOSFETs with sulfur passivation show improved subthreshold swing S, intrinsic transconductance Gm,int, and effective hole mobility μeff as compared with the non-passivated control. At a high inversion carrier density Ninv of 1 × 1013 cm−2, sulfur passivation increases μeff by 25% in Ge0.83Sn0.17 p-MOSFETs

  7. Four novel Loci (19q13, 6q24, 12q24, and 5q14 influence the microcirculation in vivo.

    M Kamran Ikram

    2010-10-01

    Full Text Available There is increasing evidence that the microcirculation plays an important role in the pathogenesis of cardiovascular diseases. Changes in retinal vascular caliber reflect early microvascular disease and predict incident cardiovascular events. We performed a genome-wide association study to identify genetic variants associated with retinal vascular caliber. We analyzed data from four population-based discovery cohorts with 15,358 unrelated Caucasian individuals, who are members of the Cohort for Heart and Aging Research in Genomic Epidemiology (CHARGE consortium, and replicated findings in four independent Caucasian cohorts (n  =  6,652. All participants had retinal photography and retinal arteriolar and venular caliber measured from computer software. In the discovery cohorts, 179 single nucleotide polymorphisms (SNP spread across five loci were significantly associated (p<5.0×10(-8 with retinal venular caliber, but none showed association with arteriolar caliber. Collectively, these five loci explain 1.0%-3.2% of the variation in retinal venular caliber. Four out of these five loci were confirmed in independent replication samples. In the combined analyses, the top SNPs at each locus were: rs2287921 (19q13; p  =  1.61×10(-25, within the RASIP1 locus, rs225717 (6q24; p = 1.25×10(-16, adjacent to the VTA1 and NMBR loci, rs10774625 (12q24; p  =  2.15×10(-13, in the region of ATXN2,SH2B3 and PTPN11 loci, and rs17421627 (5q14; p = 7.32×10(-16, adjacent to the MEF2C locus. In two independent samples, locus 12q24 was also associated with coronary heart disease and hypertension. Our population-based genome-wide association study demonstrates four novel loci associated with retinal venular caliber, an endophenotype of the microcirculation associated with clinical cardiovascular disease. These data provide further insights into the contribution and biological mechanisms of microcirculatory changes that underlie cardiovascular

  8. Synthesis and crystal structure of Li3.17(P0.69Ge0.24Mo0.07)O4

    The crystal structure of Li3.17(P0.69Ge0.24Mo0.07)O4, which was synthesized by the flux method under the action of an electric field with a potential difference of 0.8 V, was studied by X-ray diffraction on a Nonius Kappa CCD diffractometer (MoKα radiation, λ = 0.71073 A, R = 0.0137). The crystal structure is based on a tetrahedral framework typical of γ-Li3PO4. The framework of the new compound is formed by (P,Ge,Mo)O4 and LiO4 tetrahedra. The positions of additional Li atoms in the structure of Li3.17(P0.69Ge0.24Mo0.07)O4 are found to be different from those in the structure of Li3.31(P0.69Ge0.31)O4, which is synthesized in the absence of an electric field and also belongs to the γ-Li3PO4 structure type

  9. A Case of Concurrent Miller-Dieker Syndrome (17p13.3 Deletion) and 22q11.2 Deletion Syndrome.

    Atwal, Paldeep S; Macmurdo, C

    2015-12-01

    Features of Miller-Dieker syndrome (MDS, 17p13.3 deletion syndrome, LIS1-associated lissencephaly) include classic lissencephaly, microcephaly, cardiac malformations, growth restriction, and characteristic facial changes. Individuals with 22q11.2 deletion syndrome (DiGeorge syndrome or velocardiofacial syndrome) are known to have congenital cardiac malformations (in particular conotruncal defects), palatal abnormalities (especially velopharyngeal insufficiency), hypocalcemia, immune deficiency, learning disabilities, and characteristic facial features. This case report describes phenotypic characteristics of a patient with extremely rare instance of having both MDS and 22q11.2 deletion syndrome that is unique in the medical literature. Prognosis in this concurrent phenotype is poor with our patient suffering from several malformations seen in both conditions and expiring in the neonatal period. PMID:27617133

  10. Syntenic homology of human unique DNA sequences within chromossome regions 5q31, 10q22, 13q32-33 and 19q13.1 in the great apes

    Vallente-Samonte Rhea U.

    2000-01-01

    Full Text Available Homologies between chromosome banding patterns and DNA sequences in the great apes and humans suggest an apparent common origin for these two lineages. The availability of DNA probes for specific regions of human chromosomes (5q31, 10q22, 13q32-33 and 19q13.1 led us to cross-hybridize these to chimpanzee (Pan troglodytes, PTR, gorilla (Gorilla gorilla, GGO and orangutan (Pongo pygmaeus, PPY chromosomes in a search for equivalent regions in the great apes. Positive hybridization signals to the chromosome 5q31-specific DNA probe were observed at HSA 5q31, PTR 4q31, GGO 4q31 and PPY 4q31, while fluorescent signals using the chromosome 10q22-specific DNA probe were noted at HSA 10q22, PTR 8q22, GGO 8q22 and PPY 7q22. The chromosome arms showing hybridization signals to the Quint-EssentialTM 13-specific DNA probe were identified as HSA 13q32-33, PTR 14q32-33, GGO 14q32-33 and PPY 14q32-33, while those presenting hybridization signals to the chromosome 19q13.1-specific DNA probe were identified as HSA 19q13.1, PTR 20q13, GGO 20q13 and PPY 20q13. All four probes presumably hybridized to homologous chromosomal locations in the apes, which suggests a homology of certain unique DNA sequences among hominoid species.

  11. Nascent 60S ribosomal subunits enter the free pool bound by Nmd3p.

    Ho, J. H.; Kallstrom, G; Johnson, A. W.

    2000-01-01

    Nmd3p from yeast is required for the export of the large (60S) ribosomal subunit from the nucleus (Ho et al., 2000). Here, we show that Nmd3p forms a stable complex with free 60S subunits. Using an epitope-tagged Nmd3p, we show that free 60S subunits can be coimmunoprecipitated with Nmd3p. The interaction was specific for 60S subunits; 40S subunits were not coimmunoprecipitated. Using this coprecipitation technique and pulse-chase labeling of ribosomal subunit proteins we showed that Nmd3p bo...

  12. Accurate long-range coefficients for two excited like isotope He atoms: He(2 1P)-He(2 1P), He(2 1P)-He(2 3P), and He(2 3P)-He(2 3P)

    A general formalism is used to express the long-range potential energies in inverse powers of the separation distance between two like atomic or molecular systems with P symmetries. The long-range molecular interaction coefficients are calculated for the molecular symmetries Δ, Π, and Σ, arising from the following interactions: He(2 1P)-He(2 1P), He(2 1P)-He(2 3P), and He(2 3P)-He(2 3P). The electric quadrupole-quadrupole term C5, the van der Waals (dispersion) term C6, and higher-order terms C8 and C10 are calculated ab initio using accurate variational wave functions in Hylleraas coordinates with finite nuclear mass effects. A comparison is made with previously published results where available

  13. Relativistic calculations of 3s21S0-3s3p 1P1 and 3s21S0-3s3p 3P1,2 transition probabilities in the Mg isoelectronic sequence

    Using the multi-configuration Dirac—Fock self-consistent field method and the relativistic configuration-interaction method, calculations of transition energies, oscillator strengths and rates are performed for the 3s21S0-3s3p 1P1 spin-allowed transition, 3s21S0-3s3p 3P1,2 intercombination and magnetic quadrupole transition in the Mg isoelectronic sequence (Mg I, Al II, Si III, P IV and S V). Electron correlations are treated adequately, including intravalence electron correlations. The influence of the Breit interaction on oscillator strengths and transition energies are investigated. Quantum electrodynamics corrections are added as corrections. The calculation results are found to be in good agreement with the experimental data and other theoretical calculations. (atomic and molecular physics)

  14. A balanced t(5;17) (p15;q22-23) in chondroblastoma: frequency of the re-arrangement and analysis of the candidate genes

    Chondroblastoma is a benign cartilaginous tumour of bone that predominantly affects the epiphysis of long bones in young males. No recurrent chromosomal re-arrangements have so far been observed. Methods: We identified an index case with a balanced translocation by Combined Binary Ratio-Fluorescent in situ Hybridisation (COBRA-FISH) karyotyping followed by breakpoint FISH mapping and array-Comparative Genomic Hybridisation (aCGH). Candidate region re-arrangement and candidate gene expression were subsequently investigated by interphase FISH and immunohistochemistry in another 14 cases. A balanced t(5;17)(p15;q22-23) was identified. In the index case, interphase FISH showed that the translocation was present only in mononucleated cells and was absent in the characteristic multinucleated giant cells. The t(5;17) translocation was not observed in the other cases studied. The breakpoint in 5p15 occurred close to the steroid reductase 5α1 (SRD5A1) gene. Expression of the protein was found in all cases tested. Similar expression was found for the sex steroid signalling-related molecules oestrogen receptor alpha and aromatase, while androgen receptors were only found in isolated cells in a few cases. The breakpoint in 17q22-23 was upstream of the carbonic anhydrase × (CA10) gene region and possibly involved gene-regulatory elements, which was indicated by the lack of CA10 protein expression in the index case. All other cases showed variable levels of CA10 expression, with low expression in three cases. We report a novel t(5;17)(p15;q22-23) translocation in chondroblastoma without involvement of any of the two chromosomal regions in other cases studied. Our results indicate that the characteristic multinucleated giant cells in chondroblastoma do not have the same clonal origin as the mononuclear population, as they do not harbour the same translocation. We therefore hypothesise that they might be either reactive or originate from a distinct neoplastic clone, although the

  15. KⅧ-TbLⅣ离子3s3p 3P1-3s2 1S0 的自旋禁戒跃迁%Intercombination Transitions 3s3p 3P1-3s2 1S0 in Magnesium-like Ions through KⅧ to TbLⅣ

    牟致栋; 魏琦瑛

    2004-01-01

    在对KⅧ-TbLⅣ离子3s3p 3P1能级结构的多组态相互作用理论HXR方法计算的基础上, 分析了各种效应对等电子序列离子能级结构的影响, 找出了能级沿等电子序列变化的规律性. 预测计算了K Ⅷ-Tb LⅣ离子3s3p 3P1的能级. 由此进一步计算了KⅧ-TbL Ⅳ离子自旋禁戒跃迁3s3p 3P1-3s2 1S0的谱线波长、振子强度和跃迁概率.

  16. Canavan disease: Genomic organization and localization of human ASPA to 17p13-ter and conservation of the ASPA gene during evolution

    Kaul, R.; Balamurugan, K.; Gao, G.P.; Matalon, R. (Research Institute, Miami, FL (United States))

    1994-05-15

    Canavan disease, or spongy degeneration of the brain, is a severe leukodystrophy caused by the deficiency of aspartoacylase (ASPA). Recently, a missense mutation was identified in human ASPA coding sequence from patients with Canavan disease. The human ASPA gene has been cloned and found to span 29 kb of the genome. Human aspartoacylase is coded by six exons intervened by five introns. The exons vary from 94 (exon III) to 514 (exon VI) bases. The exon/intron splice junction sites follow the gt/ag consensus sequence rule. Southern blot analysis of genomic DNA from human/mouse somatic cell hybrid cell lines localized ASPA to human chromosome 17. The human ASPA locus was further mapped in the 17p13-ter region by fluorescence in situ hybridization. The bovine aspa gene has also been cloned, and its exon/intron organization is identical to that of the human gene. The 500-base sequence upstream of the initiator ATG codon in the human gene and that in the bovine gene are 77% identical. Human ASPA coding sequences cross-hybridize with genomic DNA from yeast, chicken, rabbit, cow, dog, mouse, rat, and monkey. The specificity of cross-species hybridization of coding sequences suggests that aspartoacylase has been conserved during evolution. It should now be possible to identify mutations in the noncoding genomic sequences that lead to Canavan disease and to study the regulation of ASPA. 45 refs., 4 figs., 1 tab.

  17. Syntenic homology of human unique DNA sequences within chromossome regions 5q31, 10q22, 13q32-33 and 19q13.1 in the great apes

    Vallente-Samonte Rhea U.; Conte Robert A.; Verma Ram S.

    2000-01-01

    Homologies between chromosome banding patterns and DNA sequences in the great apes and humans suggest an apparent common origin for these two lineages. The availability of DNA probes for specific regions of human chromosomes (5q31, 10q22, 13q32-33 and 19q13.1) led us to cross-hybridize these to chimpanzee (Pan troglodytes, PTR), gorilla (Gorilla gorilla, GGO) and orangutan (Pongo pygmaeus, PPY) chromosomes in a search for equivalent regions in the great apes. Positive hybridization signals to...

  18. The direct measurement of the 3 3P0-3 3P1 fine-structure interval and the gJ-factor of atomic silicon by laser magnetic resonance

    Evenson, K. M.; Beltran-Lopez, V.; Ley-Koo, E.; Inguscio, M.

    1984-01-01

    The J - 1 fine structure interval and the g-factor of the 3P1 state have been determined with high precision in the present laser magnetic resonance measurements of the ground 3p2 3P multiplet of atomic Si. Delta-E(3P1-3P0) = 2,311,755.6(7) MHz, and gJ(3P1) = 1.500830(70). Single-configuration calculations of gJ for 3P1 and 3P2 yield a value for the latter which, at 1.501095, is noted to differ by an unexpectedly large margin from the experimental value.

  19. Relativistic effects on the hyperfine structures of 2p4(3P)3p 2Do, 4Do and 4Po in 19F I

    Carette, Thomas; Nemouchi, Messaoud; Li, Jiguang; Godefroid, Michel

    2013-01-01

    The hyperfine interaction constants of the 2p4(3P)3p 2Do_{3/2,5/2}, 4Do_{1/2-7/2} and 4Po_{1/2-5/2} levels in neutral fluorine are investigated theoretically. Large-scale calculations are carried out using the multiconfiguration Hartree-Fock (MCHF) and Dirac-Hartree-Fock (MCDHF) methods. In the framework of the MCHF approach, the relativistic effects are taken into account in the Breit-Pauli approximation using non relativistic orbitals. In the fully relativistic approach, the orbitals are op...

  20. Medium Effects in Cooling of Neutron Stars and $3P_2$ Neutron Gap

    Grigorian, H.; Voskresensky, D.N.(National Research Nuclear University (MEPhI), Moscow, 115409, Russia)

    2005-01-01

    We study the dependence of the cooling of isolated neutron stars on the magnitude of the $3P_2$ neutron gap. It is demonstrated that our ``nuclear medium cooling'' scenario is in favor of a suppressed value of the $3P_2$ neutron gap.

  1. A Constitutional Translocation t(1;17)(p36.2;q11.2) in a Neuroblastoma Patient Disrupts the Human NBPF1 and ACCN1 Genes

    Staes, Katrien; Vandesompele, Jo; Laureys, Geneviève; De Smet, Els; Berx, Geert; Speleman, Frank; van Roy, Frans

    2008-01-01

    The human 1p36 region is deleted in many different types of tumors, and so it probably harbors one or more tumor suppressor genes. In a Belgian neuroblastoma patient, a constitutional balanced translocation t(1;17)(p36.2;q11.2) may have led to the development of the tumor by disrupting or activating a gene. Here, we report the cloning of both translocation breakpoints and the identification of a novel gene that is disrupted by this translocation. This gene, named NBPF1 for Neuroblastoma BreakPoint Family member 1, belongs to a recently described gene family encoding highly similar proteins, the functions of which are unknown. The translocation truncates NBPF1 and gives rise to two chimeric transcripts of NBPF1 sequences fused to sequences derived from chromosome 17. On chromosome 17, the translocation disrupts one of the isoforms of ACCN1, a potential glioma tumor suppressor gene. Expression of the NBPF family in neuroblastoma cell lines is highly variable, but it is decreased in cell lines that have a deletion of chromosome 1p. More importantly, expression profiling of the NBPF1 gene showed that its expression is significantly lower in cell lines with heterozygous NBPF1 loss than in cell lines with a normal 1p chromosome. Meta-analysis of the expression of NBPF and ACCN1 in neuroblastoma tumors indicates a role for the NBPF genes and for ACCN1 in tumor aggressiveness. Additionally, DLD1 cells with inducible NBPF1 expression showed a marked decrease of clonal growth in a soft agar assay. The disruption of both NBPF1 and ACCN1 genes in this neuroblastoma patient indicates that these genes might suppress development of neuroblastoma and possibly other tumor types. PMID:18493581

  2. A constitutional translocation t(1;17(p36.2;q11.2 in a neuroblastoma patient disrupts the human NBPF1 and ACCN1 genes.

    Karl Vandepoele

    Full Text Available The human 1p36 region is deleted in many different types of tumors, and so it probably harbors one or more tumor suppressor genes. In a Belgian neuroblastoma patient, a constitutional balanced translocation t(1;17(p36.2;q11.2 may have led to the development of the tumor by disrupting or activating a gene. Here, we report the cloning of both translocation breakpoints and the identification of a novel gene that is disrupted by this translocation. This gene, named NBPF1 for Neuroblastoma BreakPoint Family member 1, belongs to a recently described gene family encoding highly similar proteins, the functions of which are unknown. The translocation truncates NBPF1 and gives rise to two chimeric transcripts of NBPF1 sequences fused to sequences derived from chromosome 17. On chromosome 17, the translocation disrupts one of the isoforms of ACCN1, a potential glioma tumor suppressor gene. Expression of the NBPF family in neuroblastoma cell lines is highly variable, but it is decreased in cell lines that have a deletion of chromosome 1p. More importantly, expression profiling of the NBPF1 gene showed that its expression is significantly lower in cell lines with heterozygous NBPF1 loss than in cell lines with a normal 1p chromosome. Meta-analysis of the expression of NBPF and ACCN1 in neuroblastoma tumors indicates a role for the NBPF genes and for ACCN1 in tumor aggressiveness. Additionally, DLD1 cells with inducible NBPF1 expression showed a marked decrease of clonal growth in a soft agar assay. The disruption of both NBPF1 and ACCN1 genes in this neuroblastoma patient indicates that these genes might suppress development of neuroblastoma and possibly other tumor types.

  3. The duplication 17p13.3 phenotype: analysis of 21 families delineates developmental, behavioral and brain abnormalities, and rare variant phenotypes.

    Curry, Cynthia J; Rosenfeld, Jill A; Grant, Erica; Gripp, Karen W; Anderson, Carol; Aylsworth, Arthur S; Saad, Taha Ben; Chizhikov, Victor V; Dybose, Giedre; Fagerberg, Christina; Falco, Michelle; Fels, Christina; Fichera, Marco; Graakjaer, Jesper; Greco, Donatella; Hair, Jennifer; Hopkins, Elizabeth; Huggins, Marlene; Ladda, Roger; Li, Chumei; Moeschler, John; Nowaczyk, Malgorzata J M; Ozmore, Jillian R; Reitano, Santina; Romano, Corrado; Roos, Laura; Schnur, Rhonda E; Sell, Susan; Suwannarat, Pim; Svaneby, Dea; Szybowska, Marta; Tarnopolsky, Mark; Tervo, Raymond; Tsai, Anne Chun-Hui; Tucker, Megan; Vallee, Stephanie; Wheeler, Ferrin C; Zand, Dina J; Barkovich, A James; Aradhya, Swaroop; Shaffer, Lisa G; Dobyns, William B

    2013-08-01

    Chromosome 17p13.3 is a gene rich region that when deleted is associated with the well-known Miller-Dieker syndrome. A recently described duplication syndrome involving this region has been associated with intellectual impairment, autism and occasional brain MRI abnormalities. We report 34 additional patients from 21 families to further delineate the clinical, neurological, behavioral, and brain imaging findings. We found a highly diverse phenotype with inter- and intrafamilial variability, especially in cognitive development. The most specific phenotype occurred in individuals with large duplications that include both the YWHAE and LIS1 genes. These patients had a relatively distinct facial phenotype and frequent structural brain abnormalities involving the corpus callosum, cerebellar vermis, and cranial base. Autism spectrum disorders were seen in a third of duplication probands, most commonly in those with duplications of YWHAE and flanking genes such as CRK. The typical neurobehavioral phenotype was usually seen in those with the larger duplications. We did not confirm the association of early overgrowth with involvement of YWHAE and CRK, or growth failure with duplications of LIS1. Older patients were often overweight. Three variant phenotypes included cleft lip/palate (CLP), split hand/foot with long bone deficiency (SHFLD), and a connective tissue phenotype resembling Marfan syndrome. The duplications in patients with clefts appear to disrupt ABR, while the SHFLD phenotype was associated with duplication of BHLHA9 as noted in two recent reports. The connective tissue phenotype did not have a convincing critical region. Our experience with this large cohort expands knowledge of this diverse duplication syndrome. PMID:23813913

  4. Microporosity, the wetted layer and the role of CO and CO2 driving the activity of comets 29P/Schwassmann-Wachmann and 17P/Holmes

    Miles, R.

    2013-09-01

    In a previous paper [1], it was shown that melting of cometary ices will occur in the near-surface of some cometary nuclei where the escape of volatiles is restricted by a microporous matrix held together by surface tension forces: the so-called 'wetted layer'. In this paper, two distinct melting regimes are discussed, namely; (a) an 'hydrophobic' regime at heliocentric distances, rh of ~4-10 AU, where the melting of hydrocarbons and especially ethane (C2H6) ice is likely to dominate, and (b) at rh of ~1.5-4.0 AU, where oxygenated, 'hydrophilic' species are more likely to exist in the liquid phase. Literature data show that the thermophysical properties of carbon monoxide (CO) favour solution of this gas in light hydrocarbons, in particular in liquid methane (CH4) and liquid propane (C3H8) near to their melting point (85-90 K). Consequently, CO must also dissolve in liquid C2H6 at very low ambient pressures (methanol (CH3OH) and CH3OH-H2O mixtures at low temperatures and pressures, and especially when approaching freezing point. Thermal gradients within the near-surface generated by the diurnal rotation of the nucleus will concentrate gas solutes in both hydrophobic and hydrophilic environments. Temperature rise and a rapid loss in ambient pressure can result in supersaturation and an explosive release of gas leading to an associated cometary outburst. Thermal cycling can also result in the gradual migration of volatile ices from deep within a microporous nucleus. New observations of comets 29P and 17P, including new observational evidence of the anomalously slow rotation rates of their nuclei, support these hypotheses.

  5. A case of 3p deletion syndrome associated with cerebellar hemangioblastoma.

    Suzuki-Muromoto, Sato; Hino-Fukuyo, Naomi; Haginoya, Kazuhiro; Kikuchi, Atsuo; Sato, Hiroki; Sato, Yuko; Nakayama, Tojo; Kubota, Yuki; Kakisaka, Yosuke; Uematsu, Mitsugu; Kumabe, Toshihiro; Md, Shigeo Kure

    2016-02-01

    We described clinical course of a 24-year-old woman with 3p deletion syndrome associated with cerebellar hemangioblastoma at the age of 16 years old. She presented dysmorphic facial features, growth retardation and severe psychomotor retardation associated with 3p deletion syndrome. We identified de novo 3p deletion encompassing p25 by using array-based comparative genomic hybridization, where causative gene of von Hippel-Lindau (VHL) disease located. Surgical therapy for cerebellar hemangioblastoma was performed, and histological examination was consistent in cerebellar hemangioblastoma. She showed no other tumors associated VHL disease till 24 years old. This is the first case report of a patient with 3p deletion syndrome whose cerebellar hemangioblastoma may be associated with VHL disease. Repeat imaging studies were recommended for the patients with 3p deletion syndrome. PMID:26365017

  6. Further evidence for clustering of human GABA[sub A] receptor subunit genes: Localization of the [alpha][sub 6]-subunit gene (GABRA6) to distal chromosome 5q by linkage analysis

    Hicks, A.A.; Kamphuis, W.; Darlison, M.G. (MRC Molecular Neurobiology Unit, Cambride (United Kingdom)); Bailey, M.E.S.; Johnson, K.J. (Charing Cross and Westminster Medical School, London (United Kingdom)); Riley, B.P. (St. Mary' s Hospital Medical School, London (United Kingdom)); Siciliano, M.J. (Univ. of Texas M.D. Anderson Cancer Center, Houston, TX (United States))

    1994-03-15

    GABA[sub A] receptors are hetero-oligomeric ion-channel complexes that are composed of combinations of [alpha], [beta], [gamma], and [delta] subunits and play a major role in inhibitory neurotransmission in the mammalian brain. The authors report here a microsatellite polymorphism within the human [alpha][sub 6]-subunit gene (GABRA6). Mapping of this marker in a human-hamster hybrid cell-line panel and typing of the repeat in the Centre d'Etude du Polymorphisme Humain (CEPH) reference families enabled the localization of this gene to chromosome 5q and established its linkage to the GABA[sub A] receptor [alpha][sub 1]-subunit gene (GA-BRA1) with a maximum lod score (Z[sub max]) of 39.87 at a [theta] of 0.069 (males) and 0.100 (females). These results reveal the clustering of GABRA6, GABRA1, and the GABA[sub A] receptor [gamma][sub 2]-subunit gene (GABRG2) on distal chromosome 5q. 17 refs., 1 fig., 1 tab.

  7. Ge 3P 6Si 2O 25: A cage structure closely related to the intersecting tunnel structure KMo 3P 6Si 2O 25

    Leclaire, A.; Raveau, B.

    1988-08-01

    A germanosilicophosphate Ge 3P 6Si 2O 25 has been isolated. Its structure was solved from a single-crystal study in the space group P overline31c . Its cell parameters are a = b = 7.994(1) Å, c = 16.513(2) Å, Z = 2. The refinement by full-matrix least-squares calculations leads to R = 0.043 with 686 independent reflections. The structure of this oxide is built up from corner-sharing PO 4 and SiO 4 tetrahedra and GeO 6 octahedra. One observes a feature common to several silicophosphates: the presence of the structural unit P 6Si 2O 25 built up from a disilicate group sharing its corners with six PO 4 tetrahedra. The structural relationships between this oxide and the silicophosphates AMo 3P 6Si 2O 25 and Si 3P 6Si 2O 25 (or Ge 3P 6 Ge 2O 25) are described.

  8. Stark broadening of 3s 3P0-3p 3D and 3p 3D-3d 3F0 transitions along carbon isoelectronic sequences of ions revisited

    The Stark widths of N II and O III spectral lines belonging to 3s 3P0-3p 3D and 3p 3D-3d 3F0 multiplets have been calculated and measured in a capillary discharge plasma. The calculations are extended to the same multiplets of F IV and Ne V ions. The plasma electron density in the capillary discharge is determined from the width of the He II Pβ line, while the electron temperature is measured from the relative intensities of N II lines. A comparison of the experimental and theoretical data shows good mutual agreement, which is well within the estimated experimental and theoretical uncertainties. The theory predicts the ratio of the corresponding N II to O III line widths to be larger than unity, in disagreement with our earlier experiment in a pulsed arc. In the new experiment, where all spectral lines were recorded under the same plasma conditions, the theoretical predictions and the results of the capillary experiment are in agreement. The possible sources of an error in the earlier experiment are indicated

  9. Electronic and Rovibrational Quantum Chemical Analysis of C$_3$P$^-$: The Next Interstellar Anion?

    Fortenberry, Ryan C

    2015-01-01

    C$_3$P$^-$ is analogous to the known interstellar anion C$_3$N$^-$ with phosphorus replacing the nitrogen in a simple step down the periodic table. In this work, it is shown that C$_3$P$^-$ is likely to possess a dipole-bound excited state. It has been hypothesized and observationally supported that dipole-bound excited states are an avenue through which anions could be formed in the interstellar medium. Additionally, C$_3$P$^-$ has a valence excited state that may lead to further stabilization of this molecule, and C$_3$P$^-$ has a larger dipole moment than neutral C$_3$P ($\\sim 6$ D vs. $\\sim 4$ D). As such, C$_3$P$^-$ is probably a more detectable astromolecule than even its corresponding neutral radical. Highly-accurate quantum chemical quartic force fields are also applied to C$_3$P$^-$ and its singly $^{13}$C substituted isotopologues in order to provide structures, vibrational frequencies, and spectroscopic constants that may aid in its detection.

  10. Electronic and rovibrational quantum chemical analysis of C3P-: the next interstellar anion?

    Fortenberry, Ryan C.; Lukemire, Joseph A.

    2015-11-01

    C3P- is analogous to the known interstellar anion C3N- with phosphorus replacing nitrogen in a simple step down the periodic table. In this work, it is shown that C3P- is likely to possess a dipole-bound excited state. It has been hypothesized and observationally supported that dipole-bound excited states are an avenue through which anions could be formed in the interstellar medium. Additionally, C3P- has a valence excited state that may lead to further stabilization of this molecule, and C3P- has a larger dipole moment than neutral C3P (˜6 D versus ˜4 D). As such, C3P- is probably a more detectable astromolecule than even its corresponding neutral radical. Highly accurate quantum chemical quartic force fields are also applied to C3P- and its singly 13C substituted isotopologues in order to provide structures, vibrational frequencies, and spectroscopic constants that may aid in its detection.

  11. Sizeable beta-strength in {sup 31}Ar (β3p) decay

    Koldste, G.T. [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Blank, B. [Centre d' Études Nucléaire de Bordeaux-Gradignan, CNRS/IN2P3 – Université Bordeaux I, F-33175 Gradignan Cedex (France); Borge, M.J.G.; Briz, J.A.; Carmona-Gallardo, M. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); Fraile, L.M. [Grupo de Física Nuclear, Universidad Complutense, CEI Moncloa, E-28040 Madrid (Spain); Fynbo, H.O.U. [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Giovinazzo, J. [Centre d' Études Nucléaire de Bordeaux-Gradignan, CNRS/IN2P3 – Université Bordeaux I, F-33175 Gradignan Cedex (France); Johansen, J.G. [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Jokinen, A. [Department of Physics, University of Jyväskylä, FIN-40351 Jyväskylä (Finland); Jonson, B. [Fundamental Fysik, Chalmers Tekniska Högskola, S-41296 Göteborg (Sweden); Kurturkian-Nieto, T. [Centre d' Études Nucléaire de Bordeaux-Gradignan, CNRS/IN2P3 – Université Bordeaux I, F-33175 Gradignan Cedex (France); Nilsson, T. [Fundamental Fysik, Chalmers Tekniska Högskola, S-41296 Göteborg (Sweden); Perea, A.; Pesudo, V. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); and others

    2014-10-07

    We present for the first time precise spectroscopic information on the recently discovered decay mode β-delayed 3p-emission. The detection of the 3p events gives an increased sensitivity to the high energy part of the Gamow–Teller strength distribution from the decay of {sup 31}Ar revealing that as much as 30% of the strength resides in the β3p-decay mode. A simplified description of how the main decay modes evolve as the excitation energy increases in {sup 31}Cl is provided.

  12. Progress on the Multiphysics Capabilities of the Parallel Electromagnetic ACE3P Simulation Suite

    Kononenko, Oleksiy [SLAC National Accelerator Lab., Menlo Park, CA (United States)

    2015-03-26

    ACE3P is a 3D parallel simulation suite that is being developed at SLAC National Accelerator Laboratory. Effectively utilizing supercomputer resources, ACE3P has become a key tool for the coupled electromagnetic, thermal and mechanical research and design of particle accelerators. Based on the existing finite-element infrastructure, a massively parallel eigensolver is developed for modal analysis of mechanical structures. It complements a set of the multiphysics tools in ACE3P and, in particular, can be used for the comprehensive study of microphonics in accelerating cavities ensuring the operational reliability of a particle accelerator.

  13. Sizeable beta-strength in 31Ar (beta 3p) decay

    T. Koldste, G.; Blank, B.; J. G. Borge, M.;

    2014-01-01

    We present for the first time precise spectroscopic information on the recently discovered decay mode beta-delayed 3p-emission. The detection of the 3p events gives an increased sensitivity to the high energy part of the Gamow-Teller strength distribution from the decay of 31Ar revealing that as...... much as 30% of the strength resides in the beta-3p decay mode. A simplified description of how the main decay modes evolve as the excitation energy increases in 31Cl is provided....

  14. MPI spectroscopy in the region of the 3p Rydberg state of some cycloketones

    Kosmidis, C.; Boulakis, G.; Bolovinos, A.; Tsekeris, P.; Brint, P.

    1992-03-01

    The two-photon resonance three photon ionization spectra of cyclopentanone, cyclohexanone and cycloheptanone in the region of the 3p Rydberg state have been recorded, analysed and compared with the one-photon absorption spectra. A new 3p origin is identified for cyclopentanone. The absence from the MPI spectra of a sharp spectral feature that is observed in the absorption spectra is discussed. Photochemical generation of acetaldehyde is observed at high laser intensities and possible mechanisms for this are considered.

  15. miR-1207-3p Is a Novel Prognostic Biomarker of Prostate Cancer.

    Das, Dibash K; Osborne, Joseph R; Lin, Hui-Yi; Park, Jong Y; Ogunwobi, Olorunseun O

    2016-06-01

    MicroRNAs (miRNAs) have been found to be dysregulated in prostate cancer (PCa). In this study, we investigated if miR-1207-3p is capable of distinguishing between indolent and aggressive PCa and if it contributes to explaining the disproportionate aggressiveness of PCa in men of African ancestry (moAA). A total of 404 patients with primary adenocarcinoma of the prostate were recruited between 1988 and 2003 at the Moffitt Cancer Center, Tampa, FL, USA. Patient clinicopathological features and demographic characteristics such as race were identified. RNA samples from 404 postprostatectomy prostate tumor tissue samples were analyzed by real-time quantitative reverse transcription polymerase chain reaction for the mRNA expression of miR-1207-3p. miR-1207-3p expression in PCa that resulted in overall death or PCa-specific death is significantly higher than in PCa cases that did not. The same positive correlation holds true for other clinical characteristics such as biochemical recurrence, Gleason score, clinical stage, and prostate-specific antigen level. Furthermore, miR-1207-3p expression was significantly less in moAA in comparison to Caucasian men. We also evaluated whether miR-1207-3p is associated with clinical outcomes adjusted for age at diagnosis and tumor stage in the modeling. Using competing risk regression, the PCa patients with a high miR-1207-3p expression (≥6 vs 3) had a high risk to develop PCa recurrence (hazard rate = 2.5, P < .001) adjusting for age at diagnosis and tumor stage. In conclusion, miR-1207-3p is a promising novel prognostic biomarker for PCa. Furthermore, miR-1207-3p may also be important in explaining the disproportionate aggressiveness of PCa in moAA. PMID:27267842

  16. MiR-373-3p Promotes Invasion and Metastasis of Lung Adenocarcinoma Cells

    Aibing WU

    2015-07-01

    Full Text Available Background and objective Lung cancer is the leading cause of cancer-related deaths worldwide, and metastasis is the major cause of death in lung cancer patients. MiR-373 is closely associated with invasion and metastasis in other tumor cells. This study explored the expression of miR-373-3p in non-small cell lung cancer (NSCLC and its effect on the invasive and metastatic capabilities of lung adenocarcinoma cells, as well as their mechanisms of action. Methods The expression of miR-373-3p in NSCLC tissues and lung adenocarcinoma cell lines was detected by quantitative reverse transcription polymerase chain reaction. The roles of miR-373-3p in regulating lung adenocarcinoma cell invasion and metastatic properties were analyzed with miR-373-3p mimic/inhibitor-transfected cells via Transwell chamber assay. Matrix metalloproteinase MMP-9 and MMP-14 protein levels were detected by Western blot in lung cancer cells after transfection. Results MiR-373-3p was upregulated in 51 NSCLC tissues and 5 NSCLC cell lines. Gain-of-function and loss-of-function studies showed that overexpression of miR-373-3p promoted H1299 cell migration and invasion, which resulted in upregulation of MMP-9 and MMP-14. By contrast, miR-373-3p knockdown inhibited these processes in A549 cells and downregulated the expression of MMP-9 and MMP-14. Conclusion Our results demonstrated that miR-373-3p participated in the invasion and metastasis of lung adenocarcinoma cells, partly by upregulation of MMP-9 and MMP-14.

  17. Determination of diffusion, reflection and deexcitation coefficients of metastable excited Ne(3P2) atom

    Suzuki, S.; Itoh, H.

    2016-05-01

    The diffusion coefficient of the metastable excited Ne(3P2) atom in neon, the reflection coefficient of Ne(3P2) at the surface of an electrode and the rate coefficient of Ne(3P2) for collisional quenching by Ne(1S0) were determined from the gas pressure dependence of the effective lifetime of Ne(3P2). The effective lifetime of Ne(3P2) was measured from the transient current after turning off the Ultraviolet (UV) light in a Townsend discharge. The observed transient current waveform was analysed by solving the diffusion equation for the metastable excited Ne(3P2) atom using the third kind of boundary condition. The rate coefficient of Ne(3P2) for collisional quenching by Ne(1S0) and the reflection coefficient were determined by a nonspectroscopic method for the first time in neon to the best of our knowledge and were (3.2  ±  0.4)  ×  10‑16 cm3 s‑1 and 0.10  ±  0.04, respectively. The obtained diffusion coefficient at 1 Torr was 177  ±  17 cm2 s‑1, which is consistent with the value reported by Dixon and Grant. Moreover, the present results are compared with the results of Phelps and were found to be in good agreement. We also discuss the deexcitation rate of Ne(3P2) at pressures of up to 60 Torr in comparison with previously reported values.

  18. Ab-initio calculation of the 2s21S0-2s3p 3P1 intercombination transition in beryllium-like ions

    Transition probabilities of the 2s21S0-2s3p 1,3P1 intercombination and resonance lines have been calculated for 14 beryllium-like ions in the atomic range 7 ≤ Z ≤ 36. We used multiconfiguration Dirac-Fock wave functions to explore the effects of configuration interaction, of electron rearrangement as well as of relativity in a consistent scheme along the isoelectronic sequence. To show the number of configurations needed in a proper expansion of the wave functions we systematically enlarged the basis from small to large-scale. For the low-Z elements, virtual excitations of the 2s and 3p electrons up to the 5l subshells contribute significantly to the intercombination probability even though the transition energies remain almost unaffected by excitations beyond the 3l shells. The incomplete orthogonality of the orbital functions due to the rearrangement of the electron density clearly shifts the intercombination rates and appears to be independent of the configuration expansion. This effect decreases, as expected, at higher nuclear charges. (orig.)

  19. miR-193a-3p is a potential tumor suppressor in malignant pleural mesothelioma.

    Williams, Marissa; Kirschner, Michaela B; Cheng, Yuen Yee; Hanh, Jacky; Weiss, Jocelyn; Mugridge, Nancy; Wright, Casey M; Linton, Anthony; Kao, Steven C; Edelman, J James B; Vallely, Michael P; McCaughan, Brian C; Cooper, Wendy; Klebe, Sonja; Lin, Ruby C Y; Brahmbhatt, Himanshu; MacDiarmid, Jennifer; van Zandwijk, Nico; Reid, Glen

    2015-09-15

    Malignant pleural mesothelioma (MPM) is an asbestos-induced cancer with poor prognosis that displays characteristic alterations in microRNA expression. Recently it was reported that the expression of a subset of microRNAs can distinguish between MPM and adenocarcinoma of the lung. However, the functional importance of these changes has yet to be investigated. We compared expression of miR-192, miR-193a-3p and the miR-200 family in normal pleura and MPM tumor specimens and found a statistically significant reduction in the levels of miR-193a-3p (3.1-fold) and miR-192 (2.8-fold) in MPM. Transfection of MPM cells with a miR-193a-3p mimic resulted in inhibition of growth and an induction of apoptosis and necrosis in vitro. The growth inhibitory effects of miR-193a-3p were associated with a decrease in MCL1 expression and were recapitulated by RNAi-mediated MCL1 silencing. Targeted delivery of miR-193a-3p mimic using EDV minicells inhibited MPM xenograft tumour growth, and was associated with increased apoptosis. In conclusion, miR-193a-3p appears to have importance in the biology of MPM and may represent a target for therapeutic intervention. PMID:26125439

  20. Protective role of miR-23b-3p in kainic acid-induced seizure.

    Zhan, Lianbo; Yao, Yi; Fu, Huajun; Li, Zhenghui; Wang, Fengpeng; Zhang, Xiaobin; He, Wencan; Zheng, Weihong; Zhang, Yunwu; Zheng, Honghua

    2016-07-01

    Dysregulation of microRNAs has been proposed to contribute toward epilepsy. The miRNA miR-23b-3p has been found to protect against neuronal apoptosis and the production of reactive oxygen species. In this study, we assessed the potential role of miR-23b-3p in the kainic acid (KA)-induced seizure model. We found that miR-23b-3p levels were significantly decreased in the brain cortex of mice and in cultured mouse primary neurons treated with KA. Importantly, supplement of miR-23b-3p agomir by an intacerebroventricular injection alleviated seizure behaviors and abnormal cortical electroencephalogram recordings in KA-treated mice. Together, these results indicate that miR-23b-3p plays a crucial role in suppressing seizure formation in experimental models of epilepsy and that miR-23b-3p supplement may be a potential anabolic strategy for ameliorating seizure. PMID:27232518

  1. Multiplex FISH telomere integrity assay identifies an unbalanced cryptic translocation der(5)t(3;5)(q27;p15.3) in a family with three mentally retarded individuals.

    Granzow, M; Popp, S; Keller, M; Holtgreve-Grez, H; Brough, M; Schoell, B; Rauterberg-Ruland, I; Hager, H D; Tariverdian, G; Jauch, A

    2000-07-01

    Cryptic rearrangements involving the terminal regions of chromosomes are suspected to be the cause of idiopathic mental retardation in a significant number of cases. This finding highlights the necessity of a primary screening test for such chromosome aberrations. Here we present a multiplex fluorescence in situ hybridization telomere integrity assay which allows the detection of submicroscopic aberrations in the telomeric regions of all chromosomes. This novel approach identified an unbalanced cryptic translocation der(5)t(3;5)(q27;p15.3) in a family with three cases of unexplained mental retardation and dysmorphic features. The symptoms of the patients represent neither the classical dup(3q)- nor cri du chat syndrome, although all affected individuals demonstrate several features of both syndromes. The identification of two balanced translocation carriers emphasizes the significance of the telomere integrity assay for genetic counseling and prenatal diagnosis. PMID:10982035

  2. Increased miR-132-3p expression is associated with chronic neuropathic pain.

    Leinders, M; Üçeyler, N; Pritchard, R A; Sommer, C; Sorkin, L S

    2016-09-01

    Alterations in the neuro-immune balance play a major role in the pathophysiology of chronic neuropathic pain. MicroRNAs (miRNA) can regulate both immune and neuronal processes and may function as master switches in chronic pain development and maintenance. We set out to analyze the role of miR-132-3p, first in patients with peripheral neuropathies and second in an animal model of neuropathic pain. We initially determined miR-132-3p expression by measuring its levels in white blood cells (WBC) of 30 patients and 30 healthy controls and next in sural nerve biopsies of 81 patients with painful or painless inflammatory or non-inflammatory neuropathies based on clinical diagnosis. We found a 2.6 fold increase in miR-132-3p expression in WBC of neuropathy patients compared to healthy controls (panimal model of neuropathic pain, the spared nerve injury model (SNI). For this purpose miR-132-3p expression levels were measured in dorsal root ganglia and spinal cord of rats. Subsequently, miR-132-3p expression was pharmacologically modulated with miRNA antagonists or mimetics, and evoked pain and pain aversion were assessed. Spinal miR-132-3p levels were highest 10days after SNI, a time when persistent allodynia was established (pbehavior in the place escape avoidance paradigm (pbehavior in naïve rats (p<0.001). Taken together these results indicate a pro-nociceptive effect of miR-132-3p in chronic neuropathic pain. PMID:27349406

  3. Misregulation of Scm3p/HJURP causes chromosome instability in Saccharomyces cerevisiae and human cells.

    Prashant K Mishra

    2011-09-01

    Full Text Available The kinetochore (centromeric DNA and associated proteins is a key determinant for high fidelity chromosome transmission. Evolutionarily conserved Scm3p is an essential component of centromeric chromatin and is required for assembly and function of kinetochores in humans, fission yeast, and budding yeast. Overexpression of HJURP, the mammalian homolog of budding yeast Scm3p, has been observed in lung and breast cancers and is associated with poor prognosis; however, the physiological relevance of these observations is not well understood. We overexpressed SCM3 and HJURP in Saccharomyces cerevisiae and HJURP in human cells and defined domains within Scm3p that mediate its chromosome loss phenotype. Our results showed that the overexpression of SCM3 (GALSCM3 or HJURP (GALHJURP caused chromosome loss in a wild-type yeast strain, and overexpression of HJURP led to mitotic defects in human cells. GALSCM3 resulted in reduced viability in kinetochore mutants, premature separation of sister chromatids, and reduction in Cse4p and histone H4 at centromeres. Overexpression of CSE4 or histone H4 suppressed chromosome loss and restored levels of Cse4p at centromeres in GALSCM3 strains. Using mutant alleles of scm3, we identified a domain in the N-terminus of Scm3p that mediates its interaction with CEN DNA and determined that the chromosome loss phenotype of GALSCM3 is due to centromeric association of Scm3p devoid of Cse4p/H4. Furthermore, we determined that similar to other systems the centromeric association of Scm3p is cell cycle regulated. Our results show that altered stoichiometry of Scm3p/HJURP, Cse4p, and histone H4 lead to defects in chromosome segregation. We conclude that stringent regulation of HJURP and SCM3 expression are critical for genome stability.

  4. QTL Information Table: 5 [Q-TARO

    Full Text Available Physiological trait Eating quality Grain l/w ratio SSR C)Interval DH from BC3F1 Cai..., C., Bergman, C., and Oard, J.H. (2004). QTL mapping of grain quality traits from the interspecific cross Oryza sativa x O. glaberrima. Theor Appl Genet 109, 630-639. ...

  5. Identification of a rare 17p13.3 duplication including the BHLHA9 and YWHAE genes in a family with developmental delay and behavioural problems

    Capra Valeria; Mirabelli-Badenier Marisol; Stagnaro Michela; Rossi Andrea; Tassano Elisa; Gimelli Stefania; Gimelli Giorgio

    2012-01-01

    Abstract Background Deletions and duplications of the PAFAH1B1 and YWHAE genes in 17p13.3 are associated with different clinical phenotypes. In particular, deletion of PAFAH1B1 causes isolated lissencephaly while deletions involving both PAFAH1B1 and YWHAE cause Miller-Dieker syndrome. Isolated duplications of PAFAH1B1 have been associated with mild developmental delay and hypotonia, while isolated duplications of YWHAE have been associated with autism. In particular, different dysmorphic fea...

  6. Doubly excited 2s2p 1,3p1 resonances in photoionization of helium

    Wan Jian-Jie; Dong Chen-Zhong

    2009-01-01

    The multi-configuration Dirac-Fock (MCDF) method is implemented to study doubly excited 2s2p 1,3P1 resonances of the helium atom and the interference between photoionization and photoexcitation autoionization processes.In order to reproduce the total photoionization sprectra,the excited energies from the ground ls2 1 S0 state to the doubly excited 2s2p 1'3P1 states and the relevant Auger decay rates and widths are calculated in detail. Furthermore,the interference profile determined by the so-called Fano parameters q and p2 is also reproduced. Good agreement is found between the present results and other available theoretical and experimental results. This indeed shows a promising way to investigate the Fano resonances in photoionization of atoms within the MCDF scheme,although there are some discrepancies in the present calculations of the 2s2p 3P1 state.

  7. PbS/Cd3P2 quantum heterojunction colloidal quantum dot solar cells

    Here, we demonstrated the quantum heterojunction colloidal quantum dot (CQD) solar cells employing the PbS CQDs/Cd3P2 CQDs architecture in which both the p-type PbS and n-type Cd3P2 CQD layers are quantum-tunable and solution-processed light absorbers. We synthesized well-crystallized and nearly monodispersed tetragonal Cd3P2 CQDs and then engineered their energy band alignment with the p-type PbS by tuning the dot size and hence the bandgap to achieve efficient light absorbing and charge separation. We further optimized the device through the Ag-doping strategy of PbS CQDs that may leverage an expanded depletion region in the n-layer, which greatly enhances the photocurrent. The resulting devices showed an efficiency of 1.5%. (paper)

  8. A new form of Ca3P2 with a ring of Dirac nodes

    We report the synthesis and crystal structure of a new high-temperature form of Ca3P2. The crystal structure was determined through Rietveld refinements of synchrotron powder x-ray diffraction data. This form of Ca3P2 has a crystal structure of the hexagonal Mn5Si3 type, with a Ca ion deficiency compared to the ideal 5:3 stoichiometry. This yields a stable, charge-balanced compound of Ca2+ and P3−. We also report the observation of a secondary hydride phase, Ca5P3H, which again is a charge-balanced compound. The calculated band structure of Ca3P2 indicates that it is a three-dimensional Dirac semimetal with a highly unusual ring of Dirac nodes at the Fermi level. The Dirac states are protected against gap opening by a mirror plane in a manner analogous to what is seen for graphene

  9. BOREAS Level-3p Landsat TM Imagery: Geocoded and Scaled At-sensor Radiance

    Nickeson, Jaime; Knapp, David; Newcomer, Jeffrey A.; Hall, Forrest G. (Editor); Cihlar, Josef

    2000-01-01

    For BOReal Ecosystem-Atmosphere Study (BOREAS), the level-3p Landsat Thematic Mapper (TM) data were used to supplement the level-3s Landsat TM products. Along with the other remotely sensed images, the Landsat TM images were collected in order to provide spatially extensive information over the primary study areas. This information includes radiant energy, detailed land cover, and biophysical parameter maps such as Fraction of Photosynthetically Active Radiation (FPAR) and Leaf Area Index (LAI). Although very similar to the level-3s Landsat TM products, the level-3p images were processed with ground control information, which improved the accuracy of the geographic coordinates provided. Geographically, the level-3p images cover the BOREAS Northern Study Area (NSA) and Southern Study Area (SSA). Temporally, the four images cover the period of 20-Aug-1988 to 07-Jun-1994. Except for the 07-Jun-1994 image, which contains seven bands, the other three contain only three bands.

  10. Influence of Optical Properties on the Spin Polarization of Cu3P Photoelectrons

    Chassé, A.; Niebergall, L.

    We have investigated the influence of optical properties of Cu(001) on the spin polarization in Cu3p photoelectron diffraction patterns. The refraction and absorption of light have been taken into account in the calculation of the dipole transition matrix element. Therefore, a general polarization vector of light is defined within a macroscopic theory of electromagnetic fields. Results are shown and discussed for Cu3p photoelectrons excited by linearly or circularly polarized light, respectively. It is shown that the optical behavior of crystals may cause a symmetry breaking in the angular dependence of the photoelectron intensity. Besides, there are strong quantitative changes in the related spin polarization of Cu3p photoelectrons.

  11. Relativistic effects on the hyperfine structures of 2p4(3P)3p 2Do, 4Do and 4Po in 19F I

    Carette, Thomas; Li, Jiguang; Godefroid, Michel

    2013-01-01

    The hyperfine interaction constants of the 2p4(3P)3p 2Do_{3/2,5/2}, 4Do_{1/2-7/2} and 4Po_{1/2-5/2} levels in neutral fluorine are investigated theoretically. Large-scale calculations are carried out using the multiconfiguration Hartree-Fock (MCHF) and Dirac-Hartree-Fock (MCDHF) methods. In the framework of the MCHF approach, the relativistic effects are taken into account in the Breit-Pauli approximation using non relativistic orbitals. In the fully relativistic approach, the orbitals are optimized using the Dirac-Coulomb Hamiltonian with correlation models inspired by the non relativistic calculations. Higher-order excitations are captured through multireference configuration interaction calculations including the Breit interaction. In a third (intermediate) approach, the Dirac-Coulomb-Breit Hamiltonian matrix is diagonalized in a relativistic configuration space built with non relativistic MCHF radial functions converted into Dirac spinors using the Pauli approximation. The magnetic dipole hyperfine struct...

  12. Single gene microdeletions and microduplication of 3p26.3 in three unrelated families

    Kashevarova, Anna A; Nazarenko, Lyudmila P; Schultz-Pedersen, Soren; Skryabin, Nikolay A; Salyukova, Olga A; Chechetkina, Nataliya N; Tolmacheva, Ekaterina N; Rudko, Aleksey A; Magini, Pamela; Graziano, Claudio; Romeo, Giovanni; Joss, Shelagh; Tümer, Zeynep; Lebedev, Igor N

    2014-01-01

    BACKGROUND: Detection of submicroscopic chromosomal alterations in patients with a idiopathic intellectual disability (ID) allows significant improvement in delineation of the regions of the genome that are associated with brain development and function. However, these chromosomal regions usually...... contain several protein-coding genes and regulatory elements, complicating the understanding of genotype-phenotype correlations. We report two siblings with ID and an unrelated patient with atypical autism who had 3p26.3 microdeletions and one intellectually disabled patient with a 3p26.3 microduplication...

  13. MiR-373-3p Promotes Invasion and Metastasis of Lung Adenocarcinoma Cells

    Wu, Aibing; Jinmei LI; Kunpeng WU; Mo, Yanli; Luo, Yiping; Haiyin YE; Shen, Xiang; Li, Shujun; Yahai LIANG; Liu, Meilian; Yang, Zhixiong

    2015-01-01

    Background and objective Lung cancer is the leading cause of cancer-related deaths worldwide, and metastasis is the major cause of death in lung cancer patients. MiR-373 is closely associated with invasion and metastasis in other tumor cells. This study explored the expression of miR-373-3p in non-small cell lung cancer (NSCLC) and its effect on the invasive and metastatic capabilities of lung adenocarcinoma cells, as well as their mechanisms of action. Methods The expression of miR-373-3p in...

  14. Domain architectures of the Scm3p protein provide insights into centromere function and evolution

    Aravind, L.; Lakshminarayan, M. Iyer; Wu, Carl

    2007-01-01

    Recently, Scm3p has been shown to be a nonhistone component of centromeric chromatin that binds stoichiometrically to CenH3–H4 histones, and to be required for the assembly of kinetochores in S. cerevisiae. Scm3p is conserved across fungi, and displays a remarkable variation in protein size, ranging from ~200 amino acids in Saccharomyces cerevisiae to ~1300 amino acids in Neurospora crassa. This is primarily due a variable C-terminal segment that is linked to a conserved N-terminal, CenH3-int...

  15. Chromosome 3p microsatellite allelotyping in neuroblastoma: a report on the technical hurdles.

    Hoebeeck, Jasmien; De Wilde, Bram; Michels, Evi; Combaret, Valérie; Yigit, Nurten; De Smet, Els; Van Roy, Nadine; Stanbridge, Eric; Ru, Ning; Laureys, Geneviève; De Paepe, Anne; Speleman, Frank; Vandesompele, Jo

    2009-10-01

    Pinpointing critical regions of recurrent loss may help localize tumor suppressor genes. To determine the regions of loss on chromosome 3p in neuroblastoma, we performed loss of heterozygosity analysis using 16 microsatellite markers in a series of 65 primary tumors and 29 neuroblastoma cell lines. In this study, we report the results and discuss the technical hurdles that we encountered during data generation and interpretation that are of relevance for current studies or tests employing microsatellites. To provide functional support for the implication of 3p tumor suppressor genes in this childhood malignancy, we performed a microcell-mediated chromosome 3 transfer in neuroblastoma cells. PMID:19544108

  16. Absolute frequency measurement of the magnesium intercombination transition $^1S_0 \\to ^3P_1$

    Friebe, Jan; Pape, André; Riedmann, Matthias; Moldenhauer, Karsten; Mehlstäubler, Tanja; Rehbein, Nils; Lisdat, Christian; Rasel, Ernst M.; Ertmer, Wolfgang; Schnatz, Harald; Lipphardt, Burghard; Grosche, Gesine

    2007-01-01

    We report on a frequency measurement of the $(3s^2)^1S_0\\to(3s3p)^3P_1$ clock transition of $^{24}$Mg on a thermal atomic beam. The intercombination transition has been referenced to a portable primary Cs frequency standard with the help of a femtosecond fiber laser frequency comb. The achieved uncertainty is $2.5\\times10^{-12}$ which corresponds to an increase in accuracy of six orders of magnitude compared to previous results. The measured frequency value permits the calculation of several ...

  17. Electron impact investigation of the 3p-Rydberg transitions of acetone

    High resolution (15 meV), gas phase, electron impact energy loss spectra of acetone, and acetone-d6 measured as a function of incident energy and scattering angle have been used to study the anomalously weak 7.4 eV 3p-Rydberg transition in acetone. Assignments made on the basis of isotope shifts and differential electron scattering cross sections show that the weak transition observed optically and in the high energy electron impact spectrum is a forbidden transition to the out-of-plane 3p-Rydberg orbital. The band system is built on a vibronically allowed false origin enabled by the ν23 (CO bend) vibration. The analogous transition in the less symmetric molecule methyl ethyl ketone was observed to be optically allowed. All observed bands could be assigned to the one electronic transition to the out-of-plane 3p-Rydberg orbital. The other two 3p-Rydberg transitions do not appear to be active in acetone or methyl ethyl ketone. An unusual feature of this investigation was the experimental investigation of the relative differential scattering cross sections of both true and false vibronic origins within a single electronic transition. The high energy resolution allowed isotopic substitution, a traditional technique of optical spectroscopy, to be used to establish assignments in an electron impact experiment

  18. Synthesization of the Ar VIII 3s-3p beam-foil decay curve

    The beam-foil decay curve for the 3s-3p transition in Ar VIII has been simulated from experimentally determined relative initial level populations and transition probabilities calculated in the numerical Coulomb approximation. Good agreement is observed between simulated and measured decay curves. A discussion of the simulation is given. (Auth.)

  19. Canine-Origin G3P[3] Rotavirus Strain in Child with Acute Gastroenteritis

    De Grazia, Simona; Martella, Vito; Giammanco, Giovanni M; Gòmara, Miren Iturriza; Ramirez, Stefania; Cascio, Antonio; Colomba, Claudia; Arista, Serenella

    2007-01-01

    Infection by an animal-like strain of rotavirus (PA260/97) was diagnosed in a child with gastroenteritis in Palermo, Italy, in 1997. Sequence analysis of VP7, VP4, VP6, and NSP4 genes showed resemblance to a G3P[3] canine strain identified in Italy in 1996. Dogs are a potential source of human viral pathogens.

  20. miR-141-3p inhibits human stromal (mesenchymal) stem cell proliferation and differentiation

    Qiu, Weimin; Kassem, Moustapha

    2014-01-01

    Wnt signaling determines human stromal (mesenchymal) stem cell (hMSC) differentiation fate into the osteoblast or adipocyte lineage. microRNAs (miRNAs) are small RNA molecules of 21-25 nucleotides that regulate many aspects of osteoblast biology. Thus, we examined miRNAs regulated by Wnt signaling...... in hMSC. We identified miRNA (miR)-141-3p as a Wnt target which in turn inhibited Wnt signaling. Moreover, miR-141-3p inhibited hMSC proliferation by arresting cells at the G1 phase of the cell cycle. miR-141-3p inhibited osteoblast differentiation of hMSC as evidenced by reduced alkaline phosphatase...... activity, gene expression and in vitro mineralized matrix formation. Bioinformatic studies, Western blot analysis and 3'UTR reporter assay demonstrated that cell division cycle 25A (CDC25A) is a direct target of miR-141-3p. siRNA-mediated knock-down of CDC25A inhibited hMSC proliferation and osteoblast...

  1. miR-200a-3p regulates TLR1 expression in bacterial challenged miiuy croaker.

    Wang, Yanjin; Xu, Guoliang; Han, Jingjing; Xu, Tianjun

    2016-10-01

    MicroRNAs (miRNAs) are highly conserved, small non-coding RNAs which post-transcriptionally regulate various biological processes by repressing mRNA translation or degradating mRNA. It has been demonstrated that miRNAs play crucial roles in regulating the immune system. In this study, we explored the potential roles of miR-200a-3p in regulating TLR signaling pathway in miiuy croaker. Bioinformatics analysis showed that miiuy croaker TLR1 (mmiTLR1) was a putative target of miR-200a-3p. Negative expression profiles in spleen of Vibrio anguillarum challenged miiuy croaker and in lipopolysaccharide (LPS) stimulated miiuy croaker leukocytes further validated the prediction. Luciferase reporter assays showed that the dual-luciferase reporter fused to the 3'UTR of wild type mmiTLR1 cotransfected with miR-200a-3p mimics exhibited a reduction in luciferase activity compared with the controls. All of the present data provide direct evidence that miR-200a-3p is involved in TLR1 expression modulation in miiuy croaker, which will offer a basis for better understanding of miRNA regulation in fish TLR signaling pathways. PMID:27288848

  2. Linkage of atopic dermatitis to chromosomes 4q22, 3p24 and 3q21

    Christensen, Ulla; Møller-Larsen, Steffen; Nyegaard, Mette;

    2009-01-01

    Atopic dermatitis (AD) is a common, itchy skin disease of complex inheritance characterized by dermal and epidermal inflammation. The heritability is considerable and well documented. To date, four genome scans have examined the AD phenotype, showing replicated linkage at 3p26-22, 3q13-21 and 18q11...

  3. $\\beta$3p- spectroscopy and P-$\\gamma$ width determination in the decay of $^{31}$Ar

    We propose to perform a detailed study of the $\\beta$-decay of the dripline nucleus $^{31}$Ar. This will allow a detailed study of the $\\beta$-delayed 3p-decay as well as provide important information on the resonances of $^{30}$S and $^{29}$P, in particular the ratio between the P- and $\\gamma$- partial widths relevant for astrophysics.

  4. miR-511-3p Modulates Genetic Programs of Tumor-Associated Macrophages

    Mario Leonardo Squadrito

    2012-02-01

    Full Text Available Expression of the mannose receptor (MRC1/CD206 identifies macrophage subtypes, such as alternatively activated macrophages (AAMs and M2-polarized tumor-associated macrophages (TAMs, which are endowed with tissue-remodeling, proangiogenic, and protumoral activity. However, the significance of MRC1 expression for TAM's protumoral activity is unclear. Here, we describe and characterize miR-511-3p, an intronic microRNA (miRNA encoded by both mouse and human MRC1 genes. By using sensitive miRNA reporter vectors, we demonstrate robust expression and bioactivity of miR-511-3p in MRC1+ AAMs and TAMs. Unexpectedly, enforced expression of miR-511-3p tuned down the protumoral gene signature of MRC1+ TAMs and inhibited tumor growth. Our findings suggest that transcriptional activation of Mrc1 in TAMs evokes a genetic program orchestrated by miR-511-3p, which limits rather than enhances their protumoral functions. Besides uncovering a role for MRC1 as gatekeeper of TAM's protumoral genetic programs, these observations suggest that endogenous miRNAs may operate to establish thresholds for inflammatory cell activation in tumors.

  5. 3P2-3F2 pairing in dense neutron matter: the spectrum of solutions

    The 3P2-3F2 pairing model is generally considered to provide an adequate description of the superfluid states of neutron matter at densities some 2-3 times that of saturated symmetrical nuclear matter. The problem of solving the system of BCS gap equations expressing the 3P2-3F2 model is attacked with the aid of the separation approach. This method, developed originally for quantitative study of S-wave pairing in the presence of strong short-range repulsions, serves effectively to reduce the coupled, singular, nonlinear BCS integral equations to a set of coupled algebraic equations. For the first time, sufficient precision becomes accessible to resolve small energy splittings between the different pairing states. Adopting a perturbative strategy, we are able to identify and characterize the full repertoire of real solutions of the 3P2-3F2 pairing model, in the limiting regime of small tensor-coupling strength. The P-F channel coupling is seen to lift the striking parametric degeneracies revealed by a earlier separation treatment of the pure, uncoupled 3P2 pairing problem. Remarkably, incisive and robust results are obtained solely on the basis of analytic arguments. Unlike the traditional Ginzburg-Landau approach, the analysis is not restricted to the immediate vicinity of the critical temperature, but is equally reliable at zero temperature. Interesting connections and contrasts are drawn between triplet pairing in dense neutron matter and triplet pairing in liquid 3He

  6. Transition probabilities for the 3s2 3p(2P0)-3s3p2(4P) intersystem lines of Si II

    Calamai, Anthony G.; Smith, Peter L.; Bergeson, S. D.

    1993-01-01

    Intensity ratios of lines of the spin-changing 'intersystem' multiplet of S II (4P yields 2P0) at 234 nm have been used to determine electron densities and temperatures in a variety of astrophysical environments. However, the accuracy of these diagnostic calculations have been limited by uncertainties associated with the available atomic data. We report the first laboratory measurement, using an ion-trapping technique, of the radiative lifetimes of the three metastable levels of the 3s3p2 4P term of Si II. Our results are 104 +/- 16, 406 +/- 33, and 811 +/- 77 micro-s for lifetimes of the J = 1/2, 5/2, and 3/2 levels, respectively. A-values were derived from our lifetimes by use of measured branching fractions. Our A-values, which differ from calculated values by 30 percent or more, should give better agreement between modeled and observed Si II line ratios.

  7. Photoelectric characteristics of CH3NH3PbI3/p-Si heterojunction

    Yamei, Wu; Ruixia, Yang; Hanmin, Tian; Shuai, Chen

    2016-05-01

    Organic–inorganic hybrid perovskite CH3NH3PbI3 film is prepared on p-type silicon substrate using the one-step solution method to form a CH3NH3PbI3/p-Si heterojunction. The film morphology and structure are characterized by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The photoelectric properties of the CH3NH3PbI3/p-Si heterojunction are studied by testing the current–voltage (I–V) with and without illumination and capacitance–voltage (C–V) characteristics. It turns out from the I–V curve without illumination that the CH3NH3PbI3/p-Si heterojunction has a rectifier feature with the rectification ratio over 70 at the bias of ±5 V. Also, there appears a photoelectric conversion phenomenon on this heterojunction with a short circuit current (Isc) of 0.16 μA and an open circuit voltage (Voc) of about 10 mV The high frequency C–V characteristic of the Ag/CH3NH3PbI3/p-Si heterojunction turns out to be similar to that of the metal–insulator–semiconductor (MIS) structure, and a parallel translation of the C–V curve along the forward voltage axis is found. This parallel translation means the existence of defects at the CH3NH3PbI3/p-Si interface and positive fixed charges in the CH3NH3PbI3 layer. The defects at the interface of the CH3NH3PbI3/p-Si heterojunction result in the dramatic decline of the Voc. Besides, the C–V test of CH3NH3PbI3 film shows a non-linear dielectric property and the dielectric value is about 4.64 as calculated. Project supported by the Hebei Province Natural Science Foundation of China (No. F2014202184) and the Tianjin Natural Science Foundation of China (No. 15JCZDJC37800).

  8. Gamma-ray irradiation induced bulk photochromism in WO3-P2O5 glass

    Shen, Wei; Baccaro, Stefania; Cemmi, Alessia; Xu, Xiaoqing; Chen, Guorong

    2015-11-01

    In the present work, photochromism of WO3-P2O5 glass under gamma-ray irradiation was reported. As-prepared glass samples with different WO3 content are all optically transparent in the visible wavelength range thanks to the addition of a small amount of oxidizing couple Sb2O3-NaNO3. The photochromic properties are identified by transmission spectra of the glasses before and after irradiation. The results show that the irradiation induced darkening results from the reduction of W6+ to W5+ or W4+. The existence of WO6 clusters in glasses of high WO3 content is proved by XPS, which is the main reason for the obvious photochromic effects. The WO3-P2O5 glass is a promising candidate in gamma-ray sensitive detector.

  9. Parallel 3D Finite Element Particle-in-Cell Simulations with Pic3P

    Candel, A.; Kabel, A.; Lee, L.; Li, Z.; Ng, C.; Schussman, G.; Ko, K.; /SLAC; Ben-Zvi, I.; Kewisch, J.; /Brookhaven

    2009-06-19

    SLAC's Advanced Computations Department (ACD) has developed the parallel 3D Finite Element electromagnetic Particle-In-Cell code Pic3P. Designed for simulations of beam-cavity interactions dominated by space charge effects, Pic3P solves the complete set of Maxwell-Lorentz equations self-consistently and includes space-charge, retardation and boundary effects from first principles. Higher-order Finite Element methods with adaptive refinement on conformal unstructured meshes lead to highly efficient use of computational resources. Massively parallel processing with dynamic load balancing enables large-scale modeling of photoinjectors with unprecedented accuracy, aiding the design and operation of next-generation accelerator facilities. Applications include the LCLS RF gun and the BNL polarized SRF gun.

  10. Redox mechanism in the binary transition metal phosphide Cu3P

    Mauvernay, B.; Doublet, M.-L.; Monconduit, L.

    2006-05-01

    The electrochemical behaviour of the binary transition metal phosphide Cu3P towards lithium is investigated through galvano- and potentiostatic measurements. Obtained through high-temperature synthesis, this system shows a better volumetric capacity than graphite and a good capacity retention. In situ X-ray diffraction and first-principles electronic structure calculations are combined with the electrochemical results to show that the complete insertion of 3Li+ in the Cu3P electrode proceeds with the formation of three intermediate phases of lithium composition LixCu(3-x)P (x=1,2,3). The extra capacity previously observed in discharge is now clearly assigned to lithium insertion into the CuP2 impurity and to SEI reactions.

  11. Ge{sub 0.83}Sn{sub 0.17} p-channel metal-oxide-semiconductor field-effect transistors: Impact of sulfur passivation on gate stack quality

    Lei, Dian; Wang, Wei; Gong, Xiao, E-mail: elegong@nus.edu.sg, E-mail: yeo@ieee.org; Liang, Gengchiau; Yeo, Yee-Chia, E-mail: elegong@nus.edu.sg, E-mail: yeo@ieee.org [Department of Electrical and Computer Engineering, National University of Singapore, Singapore 117576 (Singapore); Zhang, Zheng; Pan, Jisheng [Institute of Material Research and Engineering, A*STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore 117602 (Singapore); Tok, Eng-Soon [Department of Physics, National University of Singapore, Singapore 117551 (Singapore)

    2016-01-14

    The effect of room temperature sulfur passivation of the surface of Ge{sub 0.83}Sn{sub 0.17} prior to high-k dielectric (HfO{sub 2}) deposition is investigated. X-ray photoelectron spectroscopy (XPS) was used to examine the chemical bonding at the interface of HfO{sub 2} and Ge{sub 0.83}Sn{sub 0.17}. Sulfur passivation is found to be effective in suppressing the formation of both Ge oxides and Sn oxides. A comparison of XPS results for sulfur-passivated and non-passivated Ge{sub 0.83}Sn{sub 0.17} samples shows that sulfur passivation of the GeSn surface could also suppress the surface segregation of Sn atoms. In addition, sulfur passivation reduces the interface trap density D{sub it} at the high-k dielectric/Ge{sub 0.83}Sn{sub 0.17} interface from the valence band edge to the midgap of Ge{sub 0.83}Sn{sub 0.17}, as compared with a non-passivated control. The impact of the improved D{sub it} is demonstrated in Ge{sub 0.83}Sn{sub 0.17} p-channel metal-oxide-semiconductor field-effect transistors (p-MOSFETs). Ge{sub 0.83}Sn{sub 0.17} p-MOSFETs with sulfur passivation show improved subthreshold swing S, intrinsic transconductance G{sub m,int}, and effective hole mobility μ{sub eff} as compared with the non-passivated control. At a high inversion carrier density N{sub inv} of 1 × 10{sup 13 }cm{sup −2}, sulfur passivation increases μ{sub eff} by 25% in Ge{sub 0.83}Sn{sub 0.17} p-MOSFETs.

  12. Chemoimmunotherapy for relapsed/refractory and progressive 17p13-deleted chronic lymphocytic leukemia (CLL) combining pentostatin, alemtuzumab, and low-dose rituximab is effective and tolerable and limits loss of CD20 expression by circulating CLL cells.

    Zent, Clive S; Taylor, Ronald P; Lindorfer, Margaret A; Beum, Paul V; LaPlant, Betsy; Wu, Wenting; Call, Timothy G; Bowen, Deborah A; Conte, Michael J; Frederick, Lori A; Link, Brian K; Blackwell, Sue E; Veeramani, Suresh; Baig, Nisar A; Viswanatha, David S; Weiner, George J; Witzig, Thomas E

    2014-07-01

    Chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL) patients with purine analog refractory disease or TP53 dysfunction still have limited treatment options and poor survival. Alemtuzumab-containing chemoimmunotherapy regimens can be effective but frequently cause serious infections. We report a Phase II trial testing the efficacy and tolerability of a short-duration regimen combining pentostatin, alemtuzumab, and low-dose high-frequency rituximab designed to decrease the risk of treatment-associated infections and to limit the loss of CD20 expression by CLL cells. The study enrolled 39 patients with progressive CLL that was either relapsed/refractory (n = 36) or previously untreated with 17p13 deletion (17p13-) (n = 3). Thirteen (33%) patients had both 17p13- and TP53 mutations predicted to be dysfunctional, and eight patients had purine analog refractory CLL without TP53 dysfunction. Twenty-six (67%) patients completed therapy, with only five (13%) patients having treatment-limiting toxicity and no treatment-related deaths. Twenty-two (56%) patients responded to treatment, with 11 (28%) complete responses (four with incomplete bone marrow recovery). Median progression-free survival was 7.2 months, time to next treatment was 9.1 months, and overall survival was 34.1 months. The majority of deaths (82%) were caused by progressive disease, including transformed diffuse large B-cell lymphoma (n = 6). Correlative studies showed that low-dose rituximab activates complement and natural killer cells without a profound and sustained decrease in expression of CD20 by circulating CLL cells. We conclude that pentostatin, alemtuzumab, and low-dose high-frequency rituximab is a tolerable and effective therapy for CLL and that low-dose rituximab therapy can activate innate immune cytotoxic mechanisms without substantially decreasing CD20 expression. PMID:24723493

  13. Ca3P2 and other topological semimetals with line nodes and drumhead surface states

    Chan, Y.-H.; Chiu, Ching-Kai; Chou, M. Y.; Schnyder, Andreas P.

    2016-05-01

    As opposed to ordinary metals, whose Fermi surfaces are two dimensional, topological (semi)metals can exhibit protected one-dimensional Fermi lines or zero-dimensional Fermi points, which arise due to an intricate interplay between symmetry and topology of the electronic wave functions. Here, we study how reflection symmetry, time-reversal symmetry, SU(2) spin-rotation symmetry, and inversion symmetry lead to the topological protection of line nodes in three-dimensional semimetals. We obtain the crystalline invariants that guarantee the stability of the line nodes in the bulk and show that a quantized Berry phase leads to the appearance of protected surfaces states, which take the shape of a drumhead. By deriving a relation between the crystalline invariants and the Berry phase, we establish a direct connection between the stability of the line nodes and the drumhead surface states. Furthermore, we show that the dispersion minimum of the drumhead state leads to a Van Hove singularity in the surface density of states, which can serve as an experimental fingerprint of the topological surface state. As a representative example of a topological semimetal, we consider Ca3P2 , which has a line of Dirac nodes near the Fermi energy. The topological properties of Ca3P2 are discussed in terms of a low-energy effective theory and a tight-binding model, derived from ab initio DFT calculations. Our microscopic model for Ca3P2 shows that the drumhead surface states have a rather weak dispersion, which implies that correlation effects are enhanced at the surface of Ca3P2 .

  14. Temperature dependence and mechanism of the reaction between O(3P) and chlorine dioxide

    Colussi, A. J.; Sander, S. P.; Fiedl, R. R.

    1992-01-01

    Second-order rate constants for the decay of O(3P) in excess chlorine dioxide, k(II), were measured as a function of total pressure (20-600 Torr argon) and temperature (248-312 K), using flash photolysis-atomic resonance fluorescence. Results indicate that k(II) is pressure dependent with a value, K(b), that is nonzero at zero pressure, and both the third-order rate constant and k(b) have negative temperature dependences.

  15. The Participative Design of an Endoscopy Facility using Lean 3P.

    Smith, Iain

    2016-01-01

    In the UK, bowel cancer is the second largest cancer killer. Diagnosing people earlier can save lives but demand for endoscopies is increasing and this can put pressure on waiting times. To address this challenge, an endoscopy unit in North East England decided to improve their facilities to increase capacity and create environments that improve the experience of users. This presented a significant opportunity for step change improvement but also a problem in terms of creating designs that meet user requirements whilst addressing structural or space constraints. The Lean design process known as '3P' (standing for the production preparation process) was utilised as a participative design strategy to engage stakeholders in the design of the new department. This involved a time-out workshop (or 3P event) in which Lean and participative design tools were utilised to create an innovative design based on 'point of delivery' (POD) principles. The team created a design that demonstrated an increase in treatment room capacity by 25% and bed capacity by 70% whilst reducing travel distance for patients by 25.8% and staff by 27.1%. This was achieved with an increase in available space of only 13%. The Lean 3P method provided a structured approach for corporate and clinical staff to work together with patient representatives as cross-functional teams. This participative approach facilitated communication and learning between stakeholders about care processes and personal preferences. Lean 3P therefore appears to be a promising approach to improving the healthcare facilities design process to meet user requirements. PMID:27493744

  16. Omega3P: A Parallel Finite-Element Eigenmode Analysis Code for Accelerator Cavities

    Lee, Lie-Quan; Li, Zenghai; Ng, Cho; Ko, Kwok; /SLAC

    2009-03-04

    Omega3P is a parallel eigenmode calculation code for accelerator cavities in frequency domain analysis using finite-element methods. In this report, we will present detailed finite-element formulations and resulting eigenvalue problems for lossless cavities, cavities with lossy materials, cavities with imperfectly conducting surfaces, and cavities with waveguide coupling. We will discuss the parallel algorithms for solving those eigenvalue problems and demonstrate modeling of accelerator cavities through different examples.

  17. Resonant inverse photoemission study of late transition metals at 3p absorption edge

    Full text: Resonant inverse photoemission spectra (RIPES) of late transition metals (TM) were observed near TM 3p absorption edge. The RIPES spectra of polycrystalline Co, Ni and Cu, as well as single crystal Ni, were observed. Figure 1 shows resonant IPES spectra of polycrystalline Co, Ni and Cu, which were prepared by evaporation. These spectra ware excited by energy above 3p absorption edge (off-resonance). Since a core hole is created by these energies, TM 3d → 3p fluorescence peak is observed in each spectrum at high energy, which is indicated by vertical bars. The main peak just above EF is TM 3d structure, while TM 4sp is observed at 10 ∼ 15 eV. In resonant spectra, the 3d structure changes its intensity, while the 4sp structure does not. In case of Ni metal, satellite structures are also observed at about 2 and 4 eV. From a calculation by Tanaka and Jo, which is based on the Anderson impurity model, the observation of satellite structures suggests the 10 ∼ 20 % 3d8 in ground state The RIPES spectra in this study give us a direct evidence of 3d8 component in Ni metal

  18. $^3P_2$-$^3F_2$ Pairing in Dense Neutron Matter The Spectrum of Solutions

    Zverev, M V; Khodel, V A

    2003-01-01

    The $^3P_2$-$^3F_2$ pairing model is generally considered to provide an adequate description of the superfluid states of neutron matter at densities some 2-3 times that of saturated symmetrical nuclear matter. The problem of solving the system of BCS gap equations expressing the $^3P_2$-$^3F_2$ model is attacked with the aid of the separation approach. This method, developed originally for quantitative study of S-wave pairing in the presence of strong short-range repulsions, serves effectively to reduce the coupled, singular, nonlinear BCS integral equations to a set of coupled algebraic equations. For the first time, sufficient precision becomes accessible to resolve small energy splittings between the different pairing states. Adopting a perturbative strategy, we are able to identify and characterize the full repertoire of real solutions of the $^3P_2$-$^3F_2$ pairing model, in the limiting regime of small tensor-coupling strength. The P-F channel coupling is seen to lift the striking parametric degeneracie...

  19. Study of the Ne(^3P_2) + CH_3F Electron Transfer Reaction below 1 Kelvin

    Jankunas, Justin; Osterwalder, Andreas

    2014-01-01

    Relatively little is known about the dynamics of electron transfer reactions at low collision energy. We present a study of Penning ionization of ground state methyl fluoride molecules by electronically excited neon atoms in the 13 $\\mu$eV--4.8 meV (150 mK--56 K) collision energy range, using a neutral-neutral merged beam setup. Relative cross sections have been measured for three Ne($^3P_2$)+ CH$_3$F reaction channels by counting the number of CH$_3$F$^+$, CH$_2$F$^+$, and CH$_3^+$ product ions, as a function of relative velocity between the neon and methyl fluoride molecular beams. Experimental cross sections markedly deviate from the Langevin capture model at collision energies above 20 K. The branching ratios are constant. In other words, the chemical shape of the CH$_3$F molecule, as seen by Ne($^3P_2$) atom, appears not to change as the collision energy is varied, in contrast to related Ne($^3P_J$) + CH$_3$X (X=Cl and Br) reactions at higher collision energies.

  20. Phase transitions in Cd3P2 at high pressures and high temperatures

    Yel'kin, F.S.; Sidorov, V.A.; Waskowska, A.;

    2008-01-01

    The high-pressure, high-temperature structural behaviour of Cd3P2 has been studied using electrical resistance measurements, differential thermal analysis, thermo baric analysis and X-ray diffraction. At room temperature, a phase transformation is observed at 4.0 GPa in compression. The experimen......The high-pressure, high-temperature structural behaviour of Cd3P2 has been studied using electrical resistance measurements, differential thermal analysis, thermo baric analysis and X-ray diffraction. At room temperature, a phase transformation is observed at 4.0 GPa in compression....... The experimental zero-pressure bulk modulus of the low-pressure phase is 64.7(7) GPa, which agrees quite well with the calculated value of 66.3 GPa using the tight-binding linear muffin-tin orbital method within the local density approximation. Tentatively, the high-pressure phase has an orthorhombic crystal...... structure with space group Pmmn (#59). The relative volume change at the phase transition is Delta V/V= -5.5%. Amorphization of the sample occurs above 25 GPa. A P-T phase diagram of Cd3P2 has been constructed. A metastable phase is observed at ambient conditions after heating the sample to above 600 K...

  1. Absolute frequency measurement of the magnesium intercombination transition $^1S_0 \\to ^3P_1$

    Friebe, Jan; Riedmann, Matthias; Moldenhauer, Karsten; Mehlstäubler, Tanja; Rehbein, Nils; Lisdat, Christian; Rasel, Ernst M; Ertmer, Wolfgang; Schnatz, Harald; Lipphardt, Burghard; Grosche, Gesine

    2007-01-01

    We report on a frequency measurement of the $(3s^2)^1S_0\\to(3s3p)^3P_1$ clock transition of $^{24}$Mg on a thermal atomic beam. The intercombination transition has been referenced to a portable primary Cs frequency standard with the help of a femtosecond fiber laser frequency comb. The achieved uncertainty is $2.5\\times10^{-12}$ which corresponds to an increase in accuracy of six orders of magnitude compared to previous results. The measured frequency value permits the calculation of several other optical transitions from $^1S_0$ to the $^3P_J$-level system for $^{24}$Mg, $^{25}$Mg and $^{26}$Mg. We describe in detail the components of our optical frequency standard like the stabilized spectroscopy laser, the atomic beam apparatus used for Ramsey-Bord\\'e interferometry and the frequency comb generator and discuss the uncertainty contributions to our measurement including the first and second order Doppler effect. An upper limit of $3\\times10^{-13}$ in one second for the short term instability of our optical f...

  2. Ypq3p-dependent histidine uptake by the vacuolar membrane vesicles of Saccharomyces cerevisiae.

    Manabe, Kunio; Kawano-Kawada, Miyuki; Ikeda, Koichi; Sekito, Takayuki; Kakinuma, Yoshimi

    2016-06-01

    The vacuolar membrane proteins Ypq1p, Ypq2p, and Ypq3p of Saccharomyces cerevisiae are known as the members of the PQ-loop protein family. We found that the ATP-dependent uptake activities of arginine and histidine by the vacuolar membrane vesicles were decreased by ypq2Δ and ypq3Δ mutations, respectively. YPQ1 and AVT1, which are involved in the vacuolar uptake of lysine/arginine and histidine, respectively, were deleted in addition to ypq2Δ and ypq3Δ. The vacuolar membrane vesicles isolated from the resulting quadruple deletion mutant ypq1Δypq2Δypq3Δavt1Δ completely lost the uptake activity of basic amino acids, and that of histidine, but not lysine and arginine, was evidently enhanced by overexpressing YPQ3 in the mutant. These results suggest that Ypq3p is specifically involved in the vacuolar uptake of histidine in S. cerevisiae. The cellular level of Ypq3p-HA(3) was enhanced by depletion of histidine from culture medium, suggesting that it is regulated by the substrate. PMID:26928127

  3. miR-342-3p affects hepatocellular carcinoma cell proliferation via regulating NF-κB pathway

    Zhao, Liang; Zhang, Yubao, E-mail: zhyb880077@sina.com

    2015-02-13

    Recent research indicates that non-coding microRNAs (miRNAs) help regulate basic cellular processes in many types of cancer cells. We hypothesized that overexpression of miR-342-3p might affect proliferation of hepatocellular carcinoma (HCC) cells. After confirming overexpression of miR-342-3p with qRT-PCR, MTT assay showed that HCC cell proliferation was significantly inhibited by miR-342-3p, and that it significantly decreased BrdU-positive cell proliferation by nearly sixfold. Searching for targets using three algorithms we found that miR-342-3p is related to the NF-κB pathway and luciferase assay found that IKK-γ, TAB2 and TAB3 are miR-342-3p target genes. Results of western blot on extracted nuclear proteins of HepG2 and HCT-116 cells showed that miR-342-3p reduced and miR-342-3p-in increased p65 nuclear levels and qRT-PCR found that NF-κB pathway downstream genes were downregulated by miR-342-3p and upregulated by miR-342-3p-in, confirming that miR-342 targets NF-κB pathway. Overexpression of Ikk-γ, TAB2 and TAB3 partially rescued HCC cells proliferation inhibited by miR-342-3p. Using the GSE54751 database we evaluated expression from 10 HCC samples, which strongly suggested downregulation of miR-342-3p and we also found inverse expression between miR-342-3p and its targets IKK-γ, TAB2 and TAB3 from 71 HCC samples. Our results show that miR-342-3p has a significant role in HCC cell proliferation and is suitable for investigation of therapeutic targets. - Highlights: • MiR-342-3p suppresses hepatocellular carcinoma cell proliferation. • MiR-342-3p targets IKK-γ, TAB2 and TAB3 genes. • MiR-342-3p downregulates NF-kB signaling pathway. • MiR-342-3p is downregulated in clinical hepatocellular carcinoma samples. • The expression of miR-342-3p and its target gene is inversely related.

  4. miR-342-3p affects hepatocellular carcinoma cell proliferation via regulating NF-κB pathway

    Recent research indicates that non-coding microRNAs (miRNAs) help regulate basic cellular processes in many types of cancer cells. We hypothesized that overexpression of miR-342-3p might affect proliferation of hepatocellular carcinoma (HCC) cells. After confirming overexpression of miR-342-3p with qRT-PCR, MTT assay showed that HCC cell proliferation was significantly inhibited by miR-342-3p, and that it significantly decreased BrdU-positive cell proliferation by nearly sixfold. Searching for targets using three algorithms we found that miR-342-3p is related to the NF-κB pathway and luciferase assay found that IKK-γ, TAB2 and TAB3 are miR-342-3p target genes. Results of western blot on extracted nuclear proteins of HepG2 and HCT-116 cells showed that miR-342-3p reduced and miR-342-3p-in increased p65 nuclear levels and qRT-PCR found that NF-κB pathway downstream genes were downregulated by miR-342-3p and upregulated by miR-342-3p-in, confirming that miR-342 targets NF-κB pathway. Overexpression of Ikk-γ, TAB2 and TAB3 partially rescued HCC cells proliferation inhibited by miR-342-3p. Using the GSE54751 database we evaluated expression from 10 HCC samples, which strongly suggested downregulation of miR-342-3p and we also found inverse expression between miR-342-3p and its targets IKK-γ, TAB2 and TAB3 from 71 HCC samples. Our results show that miR-342-3p has a significant role in HCC cell proliferation and is suitable for investigation of therapeutic targets. - Highlights: • MiR-342-3p suppresses hepatocellular carcinoma cell proliferation. • MiR-342-3p targets IKK-γ, TAB2 and TAB3 genes. • MiR-342-3p downregulates NF-kB signaling pathway. • MiR-342-3p is downregulated in clinical hepatocellular carcinoma samples. • The expression of miR-342-3p and its target gene is inversely related

  5. Identification of miR-508-3p and miR-509-3p that are associated with cell invasion and migration and involved in the apoptosis of renal cell carcinoma

    Zhai, Qingna [Department of Clinical Laboratory, Peking University Shenzhen Hospital, Guangdong (China); Shantou University Medical College, Guangdong (China); Zhou, Liang [Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Guangdong (China); Zhao, Chunjuan [Department of Clinical Laboratory, Peking University Shenzhen Hospital, Guangdong (China); Shantou University Medical College, Guangdong (China); Wan, Jun [Section of Biochemistry and Cell Biology, Division of Life Science, The Hong Kong University of Science and Technology (Hong Kong); Yu, Zhendong, E-mail: zhendongyu66@hotmail.com [Department of Clinical Laboratory, Peking University Shenzhen Hospital, Guangdong (China); Guo, Xin; Qin, Jie; Chen, Jing [Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Guangdong (China); Lu, Ruijing [Department of Clinical Laboratory, Peking University Shenzhen Hospital, Guangdong (China); Shantou University Medical College, Guangdong (China)

    2012-03-23

    Highlights: Black-Right-Pointing-Pointer Previous method was the second-generation sequencing technology. Black-Right-Pointing-Pointer miR-508-3p and miR-509-3p were significantly down-regulated in RCC tissues. Black-Right-Pointing-Pointer They can inhibit cell proliferation and migration and promote cell apoptosis. Black-Right-Pointing-Pointer The expression of miR-508-3p was significantly decreased in RCC patients plasma. Black-Right-Pointing-Pointer miR-508-3p may be a novel diagnostic marker of RCC. -- Abstract: MicroRNAs (miRNAs) have emerged as powerful regulators of multiple processes linked to human cancer, including cell apoptosis, proliferation and migration, suggesting that the regulation of miRNA function could play a critical role in cancer progression. Recent studies have found that human serum/plasma contains stably expressed miRNAs. If they prove indicative of disease states, miRNAs measured from peripheral blood samples may be a source for routine clinical detection of cancer. Our studies showed that both miR-508-3p and miR-509-3p were down-regulated in renal cancer tissues. The level of miR-508-3p but not miR-509-3p in renal cell carcinoma (RCC) patient plasma demonstrated significant differences from that in control plasma. In addition, the overexpression of miR-508-3p and miR-509-3p suppressed the proliferation of RCC cells (786-0), induced cell apoptosis and inhibited cell migration in vitro. Our data demonstrated that miR-508-3p and miR-509-3p played an important role as tumor suppressor genes during tumor formation and that they may serve as novel diagnostic markers for RCC.

  6. Identification of miR-508-3p and miR-509-3p that are associated with cell invasion and migration and involved in the apoptosis of renal cell carcinoma

    Highlights: ► Previous method was the second-generation sequencing technology. ► miR-508-3p and miR-509-3p were significantly down-regulated in RCC tissues. ► They can inhibit cell proliferation and migration and promote cell apoptosis. ► The expression of miR-508-3p was significantly decreased in RCC patients plasma. ► miR-508-3p may be a novel diagnostic marker of RCC. -- Abstract: MicroRNAs (miRNAs) have emerged as powerful regulators of multiple processes linked to human cancer, including cell apoptosis, proliferation and migration, suggesting that the regulation of miRNA function could play a critical role in cancer progression. Recent studies have found that human serum/plasma contains stably expressed miRNAs. If they prove indicative of disease states, miRNAs measured from peripheral blood samples may be a source for routine clinical detection of cancer. Our studies showed that both miR-508-3p and miR-509-3p were down-regulated in renal cancer tissues. The level of miR-508-3p but not miR-509-3p in renal cell carcinoma (RCC) patient plasma demonstrated significant differences from that in control plasma. In addition, the overexpression of miR-508-3p and miR-509-3p suppressed the proliferation of RCC cells (786-0), induced cell apoptosis and inhibited cell migration in vitro. Our data demonstrated that miR-508-3p and miR-509-3p played an important role as tumor suppressor genes during tumor formation and that they may serve as novel diagnostic markers for RCC.

  7. Theoretical study of intramultiplet transitions in collisions of atoms in 3P electronic states with structureless targets: Ca(3P)+He

    The quantum close-coupling (CC) treatment of collisions of an atom in a 3P electronic state with a structureless target is developed, based on earlier work of Mies [Phys. Rev. A 7, 942 (1973)], and a j/sub z/-conserving [coupled-states (CS)] simplification presented. There is no direct coupling between the J = 0 and J = 1 levels; transitions between these levels will occur only as a result of Coriolis coupling involving the J = 2 state. Actual CC and CS calculations are reported for collisions of Ca 43P/sup o/ with He, based on the potential curves of Malvern [J. Phys. B 11, 831 (1978)]. In the CC results, of the three independent cross sections, J = 2→1 is predicted to be largest, and J = 2→0 smallest, over the entire range of collision energies sampled. By contrast, the CS approximation predicts the 1→0 transition to be forbidden, and yields only fair accuracy for the CC 2→1 and 2→0 transitions. The coupling between spin-orbit states is also interpreted within an adiabatic model. A comparison with the experimental results of Yuh and Dagdigian (preceding paper) is made by averaging the CC cross sections over the experimental translational energy distribution. The experimental cross sections for the 2→1 and 2→0 transitions are 3--4 times larger than the theoretical values, and the 2→0 cross section is found experimentally to be approx.3 times larger than the 1→0 cross section, in direct contrast with the theoretical prediction for this ratio

  8. Thr(118Met amino acid substitution in the peripheral myelin protein 22 does not influence the clinical phenotype of Charcot-Marie-Tooth disease type 1A due to the 17p11.2-p12 duplication

    W. Marques Jr.

    2003-10-01

    Full Text Available The Thr(118Met substitution in the peripheral myelin protein 22 (PMP22 gene has been detected in a number of families with demyelinating Charcot-Marie-Tooth (CMT1 neuropathy or with the hereditary neuropathy with liability to pressure palsy, but in none of them has it consistently segregated with the peripheral neuropathy. We describe here a CMT1 family (a 63-year-old man, his brother and his niece in which two mutations on different chromosomes were found in the PMP22 gene, the 17p duplication, detected by fluorescent semiquantitative polymerase chain reaction (PCR of microsatellite markers localized within the duplicated region on chromosome 17p11.2-p12, and the Thr(118Met substitution, detected by direct sequencing the four coding exons of the PMP22 gene. A genotype/phenotype correlation study showed that the neuropathy segregates with the duplication and that the amino acid substitution does not seem to modify the clinical characteristics or the severity of the peripheral neuropathy. We did not find any evidence to characterize this substitution as a polymorphism in the population studied and we propose that the high frequency reported for this point mutation in the literature suggests that the Thr(118Met substitution may be a hotspot for mutations in the PMP22 gene.

  9. Dsj meson decay in the C{sup 3}P{sub 0} model

    Silva, D.T. da; Silva, M.L.L. da; Quadros, J.N. de; Vasconcellos, C.A.Z.; Hadjimichef, D. [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil)

    2010-07-01

    Full text. Fock-Tani is a field theory formalism appropriated for the simultaneous treatment of composite particles and their constituents. The formalism was originally developed for the treatment of problems in atomic physics and it was extended later on to the treatment of problems on hadron physics. In the Fock-Tani formalism one starts with the Fock representation of the system using field operators of elementary constituents which satisfy canonical (anti) commutation relations. Composite-particle field operators are linear combinations of the elementary-particle operators and do not generally satisfy canonical (anti) commutation relations. 'Ideal' field operators acting on an enlarged Fock space are then introduced in close correspondence with the composite ones. Next, a given unitary transformation, which transforms the single composite states into single ideal states, is introduced. Application of the unitary operator on the microscopic Hamiltonian, or on other hermitian operators expressed in terms of the elementary constituent field operators, gives equivalent operators which contain the ideal field operators. The effective Hamiltonian in the new representation has a clear physical interpretation in terms of the processes it describes. Since all field operators in the new representation satisfy canonical (anti)commutation relations, the standard methods of quantum field theory can then be readily applied. For a long time the pair creation models for strong hadronic decays have been formulated. The {sup 3}P{sub 0} model is typical decay model which considers only OZI-allowed strong decays. The {sup 3}P{sub 0} model considers a quark-antiquark par creation in the presence of the initial state meson. The quark-antiquark par is created with the vacuum quantum numbers. This model can also be obtained from the non-relativistic limit of the pair creation Hamiltonian. Applying the Fock-Tani transformation to the pair creation Hamiltonian produces the

  10. MiR-142-3p is a RANKL-dependent inducer of cell death in osteoclasts.

    Fordham, Jezrom B; Guilfoyle, Katherine; Naqvi, Afsar Raza; Nares, Salvador

    2016-01-01

    MicroRNA are small, non-coding, single-stranded RNAs that are estimated to regulate ~60% of the human genome. MiRNA profiling of monocyte-to-osteoclast differentiation identified miR-142-3p as a miRNA that is significantly, differentially expressed throughout osteoclastogenesis. Enforced expression of miR-142-3p via transient transfection with miR-142-3p mimic inhibited cell-to-cell contact and fusion, decreased protein kinase C alpha expression, and ultimately reduced cell viability. miR-142-3p was also identified as significantly differentially expressed during monocyte-to-macrophage differentiation and overexpression of miR-142-3p prevented their conversion to osteoclasts. Furthermore, the inhibitory effect of miR-142-3p on osteoclastogenesis extended to the conversion of a third osteoclast precursor cell type- dendritic cells. These results demonstrate miR-142-3p to be a negative regulator of osteoclastogenesis from the 3 main precursor cell types: monocytes, macrophages and dendritic cells. Importantly, decreased survival was dependent upon both miR-142-3p expression and RANK-signaling, with no harmful effects detected in the absence of this combination. As such, miR-142-3p represents a novel target for the selective removal of osteoclasts by targeting of osteoclastogenic pathways. PMID:27113904

  11. MicroRNA-409-3p inhibits osteosarcoma cell migration and invasion by targeting catenin-δ1.

    Wu, Shifeng; Du, Xinjie; Wu, Manwu; Du, Hechun; Shi, Xiaoliang; Zhang, Tao

    2016-06-10

    Osteosarcoma is the most common primary bone cancer which is associated with early metastatic potential and poor prognosis. However, the molecular mechanisms underlying osteosarcoma progression are not well characterized. Here, we investigated the role of miR-409-3p in osteosarcoma metastasis. Osteosarcoma tissue showed decreased expression of miR-409-3p compared to adjacent non-tumorous tissue. The expression level of miR-409-3p was negatively correlated with osteosarcoma metastasis. Overexpression of miR-409-3p in osteosarcoma cells (U2OS) inhibited cell migration and invasion. Bioinformatics analysis showed that catenin-δ1 (CTNND1, p120-catenin) is a direct target of miR-409-3p. Overexpression of miR-409-3p repressed the expression of catenin-δ1 in U2OS cells at both mRNA and protein levels. Meanwhile, miR-409-3p repressed the activity of luciferase reporter containing the 3'-untranslated region (3'UTR) of CTNND1 gene. Furthermore, expression of catenin-δ1 rescued the inhibitory effect of miR-409-3p on cell migration and invasion. Altogether, these results indicated that miR-409-3p targets catenin-δ1 to repress osteosarcoma metastasis. PMID:26992637

  12. A quasi-particle description of the M(3,p) models

    Jacob, P.; Mathieu, P

    2005-01-01

    The M(3,p) minimal models are reconsidered from the point of view of the extended algebra whose generators are the energy-momentum tensor and the primary field \\phi_{2,1} of dimension $(p-2)/4$. Within this framework, we provide a quasi-particle description of these models, in which all states are expressed solely in terms of the \\phi_{2,1}-modes. More precisely, we show that all the states can be written in terms of \\phi_{2,1}-type highest-weight states and their phi_{2,1}-descendants. We fu...

  13. Absolute frequency measurement of the 1S0 - 3P0 transition of 171Yb

    Pizzocaro, Marco; Rauf, Benjamin; Bregolin, Filippo; Milani, Gianmaria; Clivati, Cecilia; Costanzo, Giovanni A; Levi, Filippo; Calonico, Davide

    2016-01-01

    We report the absolute frequency measurement of the unperturbed transition 1S0 - 3P0 at 578 nm in 171Yb realized in an optical lattice frequency standard. The absolute frequency is measured 518 295 836 590 863.55(28) Hz relative to a cryogenic caesium fountain with a fractional uncertainty of 5.4x10-16 . This value is in agreement with the ytterbium frequency recommended as a secondary representation of the second in the International System of Units.

  14. Photoionization from the excited (3p, 3d, 4s, 4p) states of sodium

    Calculations are presented for the total cross section (σ) and the angular distribution of asymmetry parameter (β) for photoionization from initial excited (3p, 3d, 4s and 4p) states of sodium atom. Comparison of our results for σ and β is made with other available theoretical calculations and the experimental data. We also present results for the angular distributions in electron-Na+ elastic scattering at energies of k 0.5 and 1.0 a0. (author). 43 refs., 7 figs., 2 tabs

  15. Gene-centric Association Mapping of Chromosome 3p implicates MST1 in IBD pathogenesis

    Goyette, Philippe; Ng, Aylwin; Lefebvre, Céline; Brant, Steven R.; Cho, Judy H; Duerr, Richard H.; Silverberg, Mark S; Kent D. Taylor; Latiano, Anna; Aumais, Guy; Deslandres, Colette; Jobin, Gilles; Annese, Vito; Daly, Mark J.; Xavier, Ramnik J.

    2008-01-01

    Association mapping and candidate gene studies within IBD linkage regions, as well as genome-wide association studies in CD have led to the discovery of multiple risk genes, but these only explain a fraction of the genetic susceptibility observed in IBD. We have thus been pursuing a region on chromosome 3p21-22 showing linkage to CD and UC using a gene-centric association mapping approach. We identified twelve functional candidate genes by searching for literature co-citations with relevant k...

  16. Electron-phonon superconductivity in $A$Pt$_3$P compounds: from weak to strong coupling

    Subedi, Alaska; Ortenzi, Luciano; Boeri, Lilia

    2012-01-01

    We study the newly discovered Pt phosphides $A$Pt$_3$P ($A$=Sr, Ca, La) [ T. Takayama et al. Phys. Rev. Lett. 108, 237001 (2012)] using first-principles calculations and Migdal-Eliashberg theory. Given the remarkable agreement with the experiment, we exclude the charge-density wave scenario proposed by previous first-principles calculations, and give conclusive answers concerning the superconducting state in these materials. The pairing increases from La to Ca and Sr due to changes in the ele...

  17. sup 3 P sub 0 study of meson decays in a chiral quark model

    Bonnaz, R; Silvestre-Brac, B; Fernández, F; Valcarce, A

    2001-01-01

    The strong decays of a meson into two mesons are studied in the framework of the sup 3 P sub 0 model. The meson wave functions are determined by means of a realistic chiral quark model constructed in the baryon sector and comparison is made with a traditional potential of 'Coulomb + linear' type. Two different forms for the creation vertex are analyzed. A momentum dependent vertex is proved to be definitively superior. The chiral quark model provides an overall good description of all known transitions and gives results of roughly the same quality as those obtained from phenomenological quark-antiquark potentials.

  18. Diagnostic value of solitary pulmonary nodules using 99Tm-3P4-RGD2 scintigraphy

    Objective: To investigate the value of 99Tcm-HYNIC-(poly-(ethylene glycol), PEG)4-E (PEG4-c(RGDfK))2 (99Tcm-3P4-RGD2) SPECT imaging in the diagnosis of SPN by visual and semiquantitative analysis.Methods Twenty-one patients (13 men, 8 women; age range 37-77 (58±11) years) with SPN observed on CT were analyzed prospectively. All patients underwent SPECT imaging after administration of 99Tcm-3P4-RGD2 with a dose of (939 ± 118) MBq. The gold standard was based on the histopathological diagnosis of the surgical samples from all recruited patients. The diagnostic performance of CT, SPECT visual and semiquantitative analysis was compared and analyzed with ROC curves. Immunohistochemistry was performed in part of the samples to obtain the information of integrin αvβ3 expression. Informed consent was obtained from all patients. Application of a new radiopharmaceutical was permitted and approved by the local independent Ethics Committee and the Institutional Review Board of the China-Japan Union Hospital. One-way analysis of variance,two-sample t test were used with SPSS 13.0. Results: Among the 21 SPN patients, 15 cases (71%) were diagnosed as malignant, and the other 6 (29%) were benign. T/NT ratio in malignant SPN was higher than that in benign SPN (1.87 ± 0.39 vs 1.41 ± 0.65), but with no statistical significance (t=2.01, P>0.05). The sensitivities for CT, SPECT visual and semiquantitative analysis were 80% (12/15), 100% (15/15) and 100% (15/15) respectively,and the specificity were all 4/6. The AUC was 0.811 (95% CI 58%-95%) for CT, 0.833 (95% CI 61%-96%) for SPECT and 0.844 (95% CI 62%-96%) for T/NT ratios, which showed no statistical significance (F=0.83, P>0.05). Immunohistochemistry confirmed αvβ3 expression in both the malignant and benign nodules which had uptake of 99Tcm-3P4-RGD2 in SPECT imaging. Conclusion: SPECT visual and semiquantitative analysis with 99Tcm-3P4-RGD2 appears to have high sensitivity and consistency in diagnosis of SPN. (authors)

  19. Quantum chaos in ultracold collisions between Yb($^1$S$_0$) and Yb($^3$P$_2$)

    Green, Dermot G; Frye, Matthew D; Morita, Masato; Hutson, Jeremy M

    2015-01-01

    We calculate and analyze Feshbach resonance spectra for ultracold Yb($^1$S$_0$) + Yb($^3$P$_2$) collisions as a function of an interatomic potential scaling factor $\\lambda$ and external magnetic field. We show that, at zero field, the resonances are distributed randomly in $\\lambda$, but that signatures of quantum chaos emerge as a field is applied. The random zero-field distribution arises from superposition of structured spectra associated with individual total angular momenta. In addition, we show that the resonances in magnetic field in the experimentally accessible range 400 to 2000~G are chaotically distributed, with strong level repulsion that is characteristic of quantum chaos.

  20. High current Cu3P liquid metal ion source using a novel extractor configuration

    Higuchi-Rusli, R. H.; Corelli, J. C.

    1987-12-01

    It has been found that by utilizing a sharp needle for the extractor electrode in close proximity to the source tip wetted with Cu3P liquid alloy, a large increase (factor ˜300) in ion current is observed in comparison to standard liquid metal ion sources (LMIS's). In standard previously used LMIS's the extractor electrode was a flat plane with a circular hole centered on the source needle tip. This new high current source has important applications in focused and broad ion beam deposition systems.

  1. Pulsar Spin-Down by 3P2 Superfluid Neutron with Field Decay

    LUO Xin-Lian; PENG Qiu-He; CHOU Chih-Kang

    2003-01-01

    To describe pulsar spin-down, we present a simple combined torque model that takes into account both the standard magnetic dipole radiation and the electromagnetic radiation from the 3P2 superSuid vortex neutrons inside neutron star. Using an ordinary exponential model for the magnetic field decay, we derive an analytical formulae for pulsar evolution tracks. The pulsar evolution on the P-P diagram is quite different from that of the standard magnetic dipole radiation model, especially when the supernuid torque or Geld decay becomes dominant.

  2. MiR-519d-3p suppresses invasion and migration of trophoblast cells via targeting MMP-2.

    Jie Ding

    Full Text Available Our study was approved by the Medical Ethics Committee of Tang Du Hospital, Fourth Military Medical University and complied strictly with national ethical guidelines. Preeclampsia (PE is a specific clinical disorder characterized by gestational hypertension and proteinuria and is a leading cause of maternal and perinatal mortality worldwide. The miR-519d-3p is upregulated in the maternal plasma of patients with PE which indicates a possible association between this microRNA and the pathogenesis of PE. No studies to date have addressed the effect of miR-519d-3p on the invasion and migration of trophoblast cells. In our study, we found that miR-519d-3p expression was elevated in placental samples from patients with PE. In vitro, overexpression of miR-519d-3p significantly inhibited trophoblast cell migration and invasion, whereas transfection of a miR-519d-3p inhibitor enhanced trophoblast cell migration and invasion. Luciferase assays confirmed that matrix metalloproteinase-2 (MMP-2 is a direct target of miR-519d-3p. Quantitative real-time PCR and western blot assays showed that overexpression of miR-519d-3p downregulated MMP-2 mRNA and protein expression. Knockdown of MMP-2 using a siRNA attenuated the increased trophoblast migration and invasion promoted by the miR-519d-3p inhibitor. In placentas from patients with PE or normal pregnancies, a negative correlation between the expression of MMP-2 and miR-519d-3p was observed using the Pearson correlation and linear regression analysis. Our present findings suggest that upregulation of miR-519d-3p may contribute to the development of PE by inhibiting trophoblast cell migration and invasion via targeting MMP-2; miR-519d-3p may represent a potential predictive and therapeutic target for PE.

  3. Hyperfine Quenching of the 2s2p 3P0 State of Berylliumlike Ions

    Cheng, K T; Chen, M H; Johnson, W R

    2008-03-13

    The hyperfine-induced 2s2p {sup 3}P{sub 0}-2s{sup 2} {sup 1}S{sub 0} transition rate for Be-like {sup 47}Ti{sup 18+} was recently measured in a storage-ring experiment by Schippers et al. [Phys. Rev. Lett. 98, 033001 (2007)]. The measured value of 0.56(3) s{sup -1} is almost 60% larger than the theoretical value of 0.356 s{sup -1} from a multiconfiguration Dirac-Fock calculation by Marques et al. [Phys. Rev. A 47, 929 (1993)]. In this work, we use a large-scale relativistic configuration-interaction method to calculate these hyperfine-induced rates for ions with Z = 6-92. Coherent hyperfine-quenching effects between the 2s2p {sup 1,3}P{sub 1} states are included in a perturbative as well as a radiation damping approach. Contrary to the claims of Marques et al., contributions from the {sup 1}P{sub 1} state are substantial and lead to a hyperfine-induced rate of 0.67 s{sup -1}, in better agreement with, though larger than, the measured value.

  4. A New Case of an Extremely Rare 3p21.31 Interstitial Deletion.

    Lovrecic, Luca; Bertok, Sara; Žerjav Tanšek, Mojca

    2016-05-01

    Interstitial 3p21.31 deletions have been very rarely reported. We describe a 7-year-old boy with global developmental delay, specific facial characteristics, hydronephrosis, and hypothyreosis with a de novo deletion of 3p21.31, encompassing 29 OMIM genes. Despite the wide use of microarrays, no similar case has been reported in the literature so far. Five overlapping cases are deposited in the DECIPHER database, 2 of which have significant overlapping chromosomal aberrations. They both share some phenotypic characteristics with our case, e.g. developmental delay, intellectual disability and facial dysmorphism (arched eyebrows, hypertelorism, low-set ears, and a large nose tip). In addition, loss-of-function mutations in the SETD2 gene (OMIM 612778) of the deleted region have been described in 3 patients, presenting with some similar clinical features, namely overgrowth, intellectual disability, speech delay, hypotonia, autism, and epilepsy. Therefore, SETD2 may explain part of the phenotype in our case. We focused on 3 other genes in the deleted region, based on their known functions, namely CSPG5 (OMIM 606775), PTH1R (OMIM 168468) and SMARCC1 (OMIM 601732), and assessed their potentially important role in describing the patient's phenotype. Additional cases with haploinsufficiency of this region are needed to elucidate further genotype-phenotype correlations. PMID:27385966

  5. Optical potentials for Ne*(3P2,0)-Ar, N2 interactions

    Baudon, J.; Feron, P.; Miniatura, C.; Perales, F.; Reinhardt, J.; Robert, J.; Haberland, H.; Brunetti, B.; Vecchiocattivi, F.

    1991-08-01

    The differential elastic cross sections for Ne*(3P2,0)-Ar and Ne*(3P2,0)-N2 collisions have been measured in crossed beam experiments at 0.064 and 0.318 and at 0.071 and 0.295 eV, collision energies, respectively. These results have been analyzed simultaneously with integral cross sections and total ionization cross sections already available and optical spherical potentials for these two systems have been obtained. These potentials appear to be rather accurate in the distance range from 2.5 to ˜9 and from 3.0 to ˜9 Å for Ne*-Ar and Ne*-N2, respectively. The well depths and equilibrium distances are 5.12 meV and 4.9 Å for Ne*-Ar and 3.56 meV and 5.40 Å for Ne*-N2. The short-range repulsion in both cases exhibits a change in slope which can be correlated with the influence, for the interaction at shorter distances, of the Ne+ core of the metastable atom, which becomes less effective when the intermolecular distance increases. The optical potentials proposed here are given in analytical form suitable for the calculation of dynamical observables of these systems.

  6. Controlling the $2p$ Hole Alignment in Neon via the $2s$-$3p$ Fano Resonance

    Heinrich-Josties, Elisabeth; Santra, Robin

    2014-01-01

    We study the state-resolved production of neon ion after resonant photoionization of Ne via the $2s$-$3p$ Fano resonance. We find that by tuning the photon energy across the Fano resonance a surprisingly high control over the alignment of the final $2p$ hole along the polarization direction can be achieved. In this way hole alignments can be created that are otherwise very hard to achieve. The mechanism responsible for this hole alignment is the destructive interference of the direct and indirect (via the autoionizing $2s^{-1}3p$ state) ionization pathways of $2p$. By changing the photon energy the strength of the interference varies and $2p$-hole alignments with ratios up to 19:1 between $2p_0$ and $2p_{\\pm 1}$ holes can be created: an effect normally only encountered in tunnel ionization using strong-field IR pulses. Including spin-orbit interaction does not change the qualitative feature and leads only to a reduction in the alignment by $2/3$. Our study is based on a time-dependent configuration-interactio...

  7. A Mixed-Valent Molybdenum Monophosphate with a Layer Structure: KMo 3P 2O 14

    Guesdon, A.; Borel, M. M.; Leclaire, A.; Grandin, A.; Raveau, B.

    1994-03-01

    A new mixed-valent molybdenum monophosphate with a layer structure KMo 3P 2O 14 has been isolated. It crystallizes in the space group P2 1/ m with a = 8.599(2) Å, b = 6.392(2) Å, c = 10.602(1) Å, and β = 111.65(2)°. The layers [Mo 3P 2O 14] ∞ are parallel to (100) and consist of [MoPO 8] ∞ chains running along limitb→ , in which one MoO 6 octahedron alternates with one PO 4 tetrahedron. In fact, four [MoPO 8] ∞ chains share the corners of their polyhedra and the edges of their octahedra, forming [Mo 4P 4O 24] ∞ columns which are linked through MoO 5 bipyramids along limitc→. The K + ions interleaved between these layers are surrounded by eight oxygens, forming bicapped trigonal prisms KO 8. Besides the unusual trigonal bipyramids MoO 5, this structure is also characterized by a tendency to the localization of the electrons, since one octahedral site is occupied by Mo(V), whereas the other octahedral site and the trigonal bipyramid are occupied by Mo(VI). The similarity of this structure with pure octahedral layer structures suggests the possibility of generating various derivatives, and of ion exchange properties.

  8. CHARACTERIZATION AND CHROMOSOMAL ASSIGNMENT OF YEAST ARTIFICIAL CHROMOSOMES CONTAINING HUMAN 3P13-P21-SPECIFIC SEQUENCE-TAGGED SITES

    MICHAELIS, SC; BARDENHEUER, W; LUX, A; SCHRAMM, A; GOCKEL, A; SIEBERT, R; WILLERS, C; SCHMIDTKE, K; TODT, B; VANDERHOUT, AH; BUYS, CHCM; HEPPELLPARTON, AC; RABBITTS, PH; UNGAR, S; SMITH, D; LEPASLIER, D; COHEN, D; OPALKA, B; SCHUTTE, J

    1995-01-01

    Human chromosomal region 3p12-p23 is proposed to harbor at least three tumor suppressor genes involved in the development of lung cancer, renal cell carcinoma, and other neoplasias. In order to identify one of these genes we defined sequence tagged sites (STSs) specific for 3p13-p24.2 by analyzing a

  9. Interaction between 4p photoionization and 3p resonant excitation channels of krypton

    Complete text of publication follows. The angular distribution of Kr 4p photoelectrons was measured employing a linearly polarized photon beam at energies from 205 eV to 230 eV. In this range the Kr 3p→ns/md (n,m=4,5,6, etc.) resonances can be excited. The experimental anisotropy parameters (β, γ and δ) were determined for the Kr 4p shell and its fine structure components. The measurement was carried out at beamline BW3 of the DORIS III synchrotron light source at HASYLAB (Hamburg, Germany). The ESA-22D electrostatic electron spectrometer was used to analyze the emitted electrons. The spectrometer consists of a spherical and a cylindrical mirror analyzer. The spherical mirror focuses the electrons from the scattering plane to the entrance slit of the cylindrical analyzer performing the energy analysis of the electrons. (For detailed description of an ESA- 22-type electron spectrometer see Ref. [1].) The measured dipole anisotropy parameters ? of Kr 4p photoelectrons are shown in Fig. 1 as a function of photon energy. Resonance-like structure can be seen in the photon energy dependence of the dipole parameters. This structure indicates that the channel interactions are important between the 3p resonant excitation-autoionization and 4p direct photoionization processes in krypton. The natural line width of the 3p photoelectron peaks was determined from the measured spectra and it is about 0.8 eV while the experimental width of the resonance near 220 eV photon energy (3p1/2-1→5s resonant excitation) is approximately 2 eV in Fig. 1. This broadening can be explained with the strong interference between the ionization and excitation channels. As before, we conclude that the angular distribution of photoelectrons is strongly influenced by the excitation processes. Acknowledgements. The authors wish to thank the DORIS III staff for providing excellent working conditions. This work was supported by the NKTH-OTKA (Grant No. K67719), and by the European Community

  10. Unified interpretation of Hund's first and second rules for 2p and 3p atoms.

    Oyamada, Takayuki; Hongo, Kenta; Kawazoe, Yoshiyuki; Yasuhara, Hiroshi

    2010-10-28

    A unified interpretation of Hund's first and second rules for 2p (C, N, O) and 3p (Si, P, S) atoms is given by Hartree-Fock (HF) and multiconfiguration Hartree-Fock (MCHF) methods. Both methods exactly satisfy the virial theorem, in principle, which enables one to analyze individual components of the total energy E(=T+V(en)+V(ee)), where T, V(en), and V(ee) are the kinetic, the electron-nucleus attraction, and the electron-electron repulsion energies, respectively. The correct interpretation for each of the two rules can only be achieved under the condition of the virial theorem 2T+V=0 by investigating how V(en) and V(ee) interplay to attain the lower total potential energy V(=V(en)+V(ee)). The stabilization of the more stable states for all the 2p and 3p atoms is ascribed to a greater V(en) that is caused by contraction of the valence orbitals accompanied with slight expansion of the core orbitals. The contraction of the valence orbitals for the two rules is a consequence of reducing the Hartree screening of the nucleus at short interelectronic distances. The reduced screening in the first rule is due to a greater amount of Fermi hole contributions in the state with the highest total spin-angular momentum S. The reduced screening in the second rule is due to the fact that two valence electrons are more likely to be on opposite sides of the nucleus in the state with the highest total orbital-angular momentum L. For each of the two rules, the inclusion of correlation does not qualitatively change the HF interpretation, but HF overestimates the energy difference ∣ΔE∣ between two levels being compared. The magnitude of the correlation energy is significantly larger for the lower L states than for the higher L states since two valence electrons in the lower L states are less likely to be on opposite sides of the nucleus. The MCHF evaluation of ∣ΔE∣ is in excellent agreement with experiment. The present HF and MCHF calculations demonstrate the above statements

  11. MicroRNA-490-3p inhibits proliferation of A549 lung cancer cells by targeting CCND1

    Highlights: • We examined the level of miR-490-3p in A549 lung cancer cells compared with normal bronchial epithelial cell line. • We are the first to show the function of miR-490-3p in A549 lung cancer cells. • We demonstrate CCND1 may be one of the targets of miR-490-3p. - Abstract: MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate the translation of messenger RNAs by binding their 3′-untranslated region (3′UTR). In this study, we found that miR-490-3p is significantly down-regulated in A549 lung cancer cells compared with the normal bronchial epithelial cell line. To better characterize the role of miR-490-3p in A549 cells, we performed a gain-of-function analysis by transfecting the A549 cells with chemically synthesized miR-490-3P mimics. Overexpression of miR-490-3P evidently inhibits cell proliferation via G1-phase arrest. We also found that forced expression of miR-490-3P decreased both mRNA and protein levels of CCND1, which plays a key role in G1/S phase transition. In addition, the dual-luciferase reporter assays indicated that miR-490-3P directly targets CCND1 through binding its 3′UTR. These findings indicated miR-490-3P could be a potential suppressor of cellular proliferation

  12. Functional Expression and Characterization of Schizosaccharomyces pombe Avt3p as a Vacuolar Amino Acid Exporter in Saccharomyces cerevisiae.

    Soracom Chardwiriyapreecha

    Full Text Available In Saccharomyces cerevisiae, Avt3p and Avt4p mediate the extrusion of several amino acids from the vacuolar lumen into the cytosol. SpAvt3p of Schizosaccharomyces pombe, a homologue of these vacuolar amino acid transporters, has been indicated to be involved in spore formation. In this study, we confirmed that GFP-SpAvt3p localized to the vacuolar membrane in S. pombe. The amounts of various amino acids increased significantly in the vacuolar pool of avt3Δ cells, but decreased in that of avt3+-overexpressing avt3Δ cells. These results suggest that SpAvt3p participates in the vacuolar compartmentalization of amino acids in S. pombe. To examine the export activity of SpAvt3p, we expressed the avt3+ gene in S. cerevisiae cells. We found that the heterologously overproduced GFP-SpAvt3p localized to the vacuolar membrane in S. cerevisiae. Using the vacuolar membrane vesicles isolated from avt3+-overexpressing S. cerevisiae cells, we detected the export activities of alanine and tyrosine in an ATP-dependent manner. These activities were inhibited by the addition of a V-ATPase inhibitor, concanamycin A, thereby suggesting that the activity of SpAvt3p is dependent on a proton electrochemical gradient generated by the action of V-ATPase. In addition, the amounts of various amino acids in the vacuolar pools of S. cerevisiae cells were decreased by the overproduction of SpAvt3p, which indicated that SpAvt3p was functional in S. cerevisiae cells. Thus, SpAvt3p is a vacuolar transporter that is involved in the export of amino acids from S. pombe vacuoles.

  13. Spin structure of binary processes in the 3P0 model of coloured tube

    The binary processes 0+1/2 →0+1/2 (π-p → π0n, π-p → ηn) are considered in the coloured tube model. It is shown that the relations of residues of the amplitude M0+iσ-vectorn-vectorq/2q0M1 and their q2-dependence at the secondary Regge pole exchange is determined by the wave function of a gg-bar pair which appears in the 3P0 state at disruption of a coloured tube. The account of the Thomas interaction gives a small contribution into matrix elements logarithmically decreasing with energy. The model, accounting for absorption, agrees semiquantitatively with experimental data

  14. Product lambda-doublet ratios for the O(3P) + D2 reaction: A mechanistic imprint

    Jambrina, P G; Aldegunde, J; Brouard, M; Aoiz, F J

    2016-01-01

    In the last decade, the development of theoretical methods have allowed chemists to reproduce and explain almost all of the experimental data associated with elementary atom plus diatom collisions. However, there are still a few examples where theory cannot account yet for experimental results. This is the case for the preferential population of one of the $\\Lambda$-doublet states produced by chemical reactions. In particular, recent measurements of the OD($^2\\Pi$) product of the O($^3$P) + D$_2$ reaction have shown a clear preference for the $\\Pi(A')$ $\\Lambda$-doublet states, in apparent contradiction with {\\em ab initio} calculations, which predict a larger reactivity on the $A"$ potential energy surface. Here we present a method to calculate the $\\Lambda$-doublet ratio when concurrent potential energy surfaces participate in the reaction. It accounts for the experimental $\\Lambda$-doublet populations via explicit consideration of the stereodynamics of the process. Furthermore, our results demonstrate that...

  15. Lanthanide refractory semiconductors based on the Th/sub 3/P/sub 4/ structure

    The phase relationships and the important structural, electrical and thermal properties of the R/sub 3/X/sub 4/-R/sub 2/X/sub 3/ (where R = lanthanides and X = S, Se and Te) phases having the Th/sub 3/P/sub 4/-type structure are reviewed. The room temperature electrical resistivity and Seebeck coefficient of these materials are independent of R and only slightly dependent on X, but critically dependent on the X:R ratio. The long term stability of these phases is also reviewed. Although these materials have good thermoelectric properties there are some problems which need to be solved before these phases can be utilized in thermoelectric devices. These problems include long term stability, higher than desirable thermal conductivities, and low electron mobilities

  16. Mechanisms and Kinetics of Radical Reaction of O(1D,3P) + HCN System

    HUANG Yu-Cheng; DU Jin-Yan; JU Xue-Hai; YE Shi-Yong; ZHOU Tao

    2008-01-01

    The reaction of HCN with O(1D, 3P) radical has been investigated by density functional theory (DFT) and ab initio methods. The stationary points on the reaction paths(reactants, intermediates and products) were optimized at the (U)B3LYP/aug-cc-pVTZ level.Single-point calculations were performed at the (U)QCISD(T)/aug-cc-pVTZ level for the optimized structures and all the total energies were corrected by zero-point energy. It is shown that there exist three competing mechanisms of oxygen attacking nitrogen O→N, oxygen attacking carbon O→C and oxygen attacking hydrogen O→H. The rate constants were obtained via Eyring transition-state theory in the temperature range of 600~2000 K. The linear relationship between lnk and 1/T was presented. The results show that path 1 is the main reaction channel and the product of NCO + H is predominant.

  17. High strength Al–Al2O3p composites: Optimization of extrusion parameters

    Luan, B.F.; Hansen, Niels; Godfrey, A.;

    2011-01-01

    Composite aluminium alloys reinforced with Al2O3p particles have been produced by squeeze casting followed by hot extrusion and a precipitation hardening treatment. Good mechanical properties can be achieved, and in this paper we describe an optimization of the key processing parameters. The...... investigation of their mechanical properties and microstructure, as well as on the surface quality of the extruded samples. The evaluation shows that material with good strength, though with limited ductility, can be reliably obtained using a production route of squeeze casting, followed by hot extrusion and a...... precipitation hardening treatment. For the extrusion step optimized processing parameters have been determined as: (i) extrusion temperature=500°C–560°C; (ii) extrusion rate=5mm/s; (iii) extrusion ratio=10:1....

  18. Electron impact excitation of 2p and 3p states of hydrogen at intermediate energies

    A coupled-channel-optical (CCO) calculation with 9 and 12 atomic states supplemented with the continuum optical potentials for the stronger coupling channels has been performed for the electron impact excitation of hydrogen. The calculated n = 2 and n = 3 differential cross sections and the reduced Stokes parameters are comparable with the state-of-the art calculations. There is closer agreement between the present calculations and the experimental measurements for the reduced Stokes parameters P-bar1 and P-bar3 in the n = 2p excitation at 54.4 eV. The present CCO calculations also display good accord with the limited experimental data for the reduced Stokes parameters in the n = 3p excitation. (authors)

  19. Precision Hyperfine Structure of 2;^3P State of ^3He with External Magnetic

    Wu, Qixue; Drake, G. W. F.

    2007-06-01

    The theory of the Zeeman effect can be used to extrapolate precise measurements for the fine structure or the hyperfine structure to zero-field strength. In the present work, the hyperfine structure of 2;^3P state of ^3He with external magnetic fields is precisely calculated. The values of the fields for 32 crossings and five anticrossings of the magnetic sublevels are theoretically predicted for magnetic field strengths up to 1 Tesla. The results are compared with experimental work. We include the linear terms, diamagnetic terms, and the 2̂ relativistic correction terms in the Zeeman Hamiltonian. All related matrix elements are calculated with high accuracy by the use of double basis set Hylleraas type variational wave functions[1,2].[1] Z. -C. Yan and G.W.F. Drake, Phys. Rev. A 50, R1980 (1994).[2] Q. Wu and G.W.F. Drake, J. Phys. B 40, 393 (2007).

  20. Expression of miR-199a-3p in human adipocytes is regulated by free fatty acids and adipokines.

    Gu, Nan; You, Lianghui; Shi, Chunmei; Yang, Lei; Pang, Lingxia; Cui, Xianwei; Ji, Chenbo; Zheng, Wen; Guo, Xirong

    2016-08-01

    Obesity is associated with a notable risk for disease, including risk of cardiovascular disorders, type 2 diabetes mellitus (T2DM) and hypertension. Adipose tissue modulates the metabolism by releasing free fatty acids (FFAs) and adipokines, including leptin, resistin, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL‑6). Altered secretion patterns of FFAs and adipokines have been demonstrated to result in obesity‑associated insulin resistance (IR) and inflammatory responses. MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM. However, the association between miR-199a-3p levels in adipocytes and obesity‑associated IR, as well as inflammatory responses remains to be elucidated. The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes. In addition, miR‑199a‑3p expression was higher in visceral adipose deposits from obese subjects. FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses. PMID:27279151

  1. miR-224-3p inhibits autophagy in cervical cancer cells by targeting FIP200.

    Fang, Wang; Shu, Shan; Yongmei, Li; Endong, Zhu; Lirong, Yin; Bei, Sun

    2016-01-01

    Cervical cancer (CC) is a malignant solid tumor, which is one of the main causes of morbidity and mortality in women. Persistent High-risk human papillomavirus (hrHPV) infection is closely related to cervical cancer and autophagy has been suggested to inhibit viral infections. miRNAs have been reported to regulate autophagy in many solid tumors with many studies implicating miR-224-3p in the regulation of autophagy. In this study, we performed a miRNA microarray analysis on CC tissues and found that a large number of miRNAs with differential expressions in hrHPV-infected tissues. We identified miR-224-3p as a candidate miRNA selectively up regulated in HPV-infected tissues and cell lines. Further analysis revealed that miR-224-3p regulates autophagy in cervical cancer tissues and cell lines. While the overexpression of miR-224-3p inhibits autophagy in HPV-infected cells, knocking down endogenous miR-224-3p increases autophagy activity in the same cells. In addition, we found that miR-224-3p directly inhibits the expression of autophagy related gene, FAK family-interacting protein of 200 kDa (FIP200). In summary, we found that miR-224-3p regulates autophagy in hrHPV-induced cervical cancer cells through targeting FIP200 expression. PMID:27615604

  2. MicroRNA-23a-3p promotes the development of osteoarthritis by directly targeting SMAD3 in chondrocytes.

    Kang, Liang; Yang, Cao; Song, Yu; Liu, Wei; Wang, Kun; Li, Shuai; Zhang, Yukun

    2016-09-01

    Osteoarthritis (OA) is a common chronic degenerative joint disease. Progressive destruction of the integrity of articular cartilage is an important pathological feature, but treatment options that reverse this damage have not been developed. According to recent studies, microRNAs have important regulatory roles in the initiation and progression of OA. In the current study, the biological effects of miR-23a-3p and its expression in OA tissues were examined. We found that miR-23a-3p expression was obviously higher and SMAD3 expression was significantly lower in OA cartilage than in normal tissues. The hypomethylation status of CpG islands in the promoter region of miR-23a-3p was confirmed by methylation-specific polymerase chain reaction in OA cartilage tissues. Furthermore, a bioinformatics analysis and luciferase reporter assay identified SMAD3 as a target gene of miR-23a-3p and SMAD3 expression at both the protein and mRNA levels was inhibited by miR-23a-3p. A functional analysis demonstrated that miR-23a-3p overexpression suppresses type II collagen and aggrecan expression, while miR-23a-3p inhibition had the opposite effects. Small interfering RNA-mediated knockdown of SMAD3 reversed the effects of the miR-23a-3p inhibitor on the expression of type II collagen and aggrecan. Our results suggested that miR-23a-3p contributes to OA progression by directly targeting SMAD3, providing a potential therapeutic target for OA treatment. PMID:27318087

  3. miR-208-3p promotes hepatocellular carcinoma cell proliferation and invasion through regulating ARID2 expression

    MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at post-transcriptional level. miRNA dysregulation plays a causal role in cancer progression. In this study, miR-208-3p was highly expressed and directly repressed ARID2 expression. As a result, ARID2 expression in hepatocellular carcinoma (HCC) was decreased. In vitro, miR-208-3p down-regulation and ARID2 over-expression elicited similar inhibitory effects on HCC cell proliferation and invasion. In vivo test results revealed that miR-208-3p down-regulation inhibited HCC tumorigenesis in Hep3B cells. Moreover, ARID2 was possibly a downstream element of transforming growth factor beta1 (TGFβ1)/miR-208-3p/ARID2 regulatory pathway. These findings suggested that miR-208-3p up-regulation is associated with HCC cell progression and may provide a new target for liver cancer treatment. - Highlights: • miR-208-3p was highly expressed and directly repressed the expression of ARID2 in HCC. • miR-208-3p contributed to HCC cell progression both in vitro and in vivo. • Over-expression of ARID2 inhibited the HCC cell proliferation and invasion. • Restoration of ARID2 partly reversed the the effect of miR-208-3p down-regulation on HCC cells. • Newly regulatory pathway: miR-208-3p mediated the repression of ARID2 by TGFβ1 in HCC cells

  4. miR-208-3p promotes hepatocellular carcinoma cell proliferation and invasion through regulating ARID2 expression

    Yu, Peng; Wu, Dingguo; You, Yu; Sun, Jing; Lu, Lele; Tan, Jiaxing; Bie, Ping, E-mail: bieping2010@163.com

    2015-08-15

    MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at post-transcriptional level. miRNA dysregulation plays a causal role in cancer progression. In this study, miR-208-3p was highly expressed and directly repressed ARID2 expression. As a result, ARID2 expression in hepatocellular carcinoma (HCC) was decreased. In vitro, miR-208-3p down-regulation and ARID2 over-expression elicited similar inhibitory effects on HCC cell proliferation and invasion. In vivo test results revealed that miR-208-3p down-regulation inhibited HCC tumorigenesis in Hep3B cells. Moreover, ARID2 was possibly a downstream element of transforming growth factor beta1 (TGFβ1)/miR-208-3p/ARID2 regulatory pathway. These findings suggested that miR-208-3p up-regulation is associated with HCC cell progression and may provide a new target for liver cancer treatment. - Highlights: • miR-208-3p was highly expressed and directly repressed the expression of ARID2 in HCC. • miR-208-3p contributed to HCC cell progression both in vitro and in vivo. • Over-expression of ARID2 inhibited the HCC cell proliferation and invasion. • Restoration of ARID2 partly reversed the the effect of miR-208-3p down-regulation on HCC cells. • Newly regulatory pathway: miR-208-3p mediated the repression of ARID2 by TGFβ1 in HCC cells.

  5. Genomic characterization of uncommon human G3P[6] rotavirus strains causing diarrhea in children in Italy in 2009.

    Ianiro, Giovanni; Delogu, Roberto; Fiore, Lucia; Ruggeri, Franco M

    2015-07-01

    Group A rotaviruses (RVA) are the leading cause of acute gastroenteritis in young children, causing up to 450,000 deaths worldwide, mostly in developing countries. Most of RVA human infections in developed countries are related to five major G/P combinations: G1P[8], G2P[4], G3P[8], G4P[8] and G9P[8]. During the surveillance activity of RotaNet-Italy, three uncommon G3P[6] RVA strains, designated as RVA/Human-wt/ITA/NA01/2009/G3P[6], RVA/Human-wt/ITA/NA06/2009/G3P[6], and RVA/Human-wt/ITA/NA19/2009/G3P[6], were identified in the stools of children with diarrhea hospitalized in Southern Italy in 2009. Samples NA01, NA06 and NA19 were characterized as genotype G3P[6]. To investigate the three strains further, partial sequencing of the eleven genomic segments was performed. RVA strains NA01, NA06 and NA19 were found to share the rare genotype constellation: G3-P[6]-I2-R2-C2-M2-A2-N2-T2-E2-H2, which had not been reported previously in continental Italy. The phylogenetic analysis of the eleven genomic segments showed no evidence of zoonosis or inter-species reassortment at the origin of the Italian G3P[6] strains, indicating that they possessed DS-1-like genomic constellations similar to those detected previously in human cases in Africa and Europe. The analysis of the hypervariable regions of VP7 and VP4 (VP8*) revealed high amino acid identity between the Italian G3P[6] RVA strains involved in this study. PMID:25913157

  6. Hyperfine Quenching of the $4s4p ^{3}P_{0}$ Level in Zn-like Ions

    Marques, J P; Indelicato, Paul

    2007-01-01

    In this paper, we used the multiconfiguration Dirac-Fock method to compute with high precision the influence of the hyperfine interaction on the $[Ar]3d^{10} 4s4p ^3P_0$ level lifetime in Zn-like ions for stable and some quasi-stable isotopes of nonzero nuclear spin between Z=30 and Z=92. The influence of this interaction on the $[Ar]3d^{10} 4s4p ^3P_1 - [Ar]3d^{10} 4s4p ^3P_0$ separation energy is also calculated for the same ions.

  7. Energy-band alignment of II-VI/Zn3P2 heterojunctions from x-ray photoemission spectroscopy

    Watson, Graeme

    2013-01-01

    PUBLISHED The energy-band alignments for zb-ZnSe(001)/ a -Zn 3 P 2 (001), w-CdS(0001)/ a -Zn 3 P 2 (001), and w-ZnO(0001)/ a -Zn 3 P 2 (001) heterojunctions have been determined using high-resolution x-ray photoelectron spectroscopy via the Kraut method. Ab initio hybrid density functional theory calculations of the valence-band density of states were used to determine the energy differences between the core level and ...

  8. Wakefield Computations for the CLIC PETS using the Parallel Finite Element Time-Domain Code T3P

    Candel, A; Kabel, A.; Lee, L.; Li, Z.; Ng, C.; Schussman, G.; Ko, K.; /SLAC; Syratchev, I.; /CERN

    2009-06-19

    In recent years, SLAC's Advanced Computations Department (ACD) has developed the high-performance parallel 3D electromagnetic time-domain code, T3P, for simulations of wakefields and transients in complex accelerator structures. T3P is based on advanced higher-order Finite Element methods on unstructured grids with quadratic surface approximation. Optimized for large-scale parallel processing on leadership supercomputing facilities, T3P allows simulations of realistic 3D structures with unprecedented accuracy, aiding the design of the next generation of accelerator facilities. Applications to the Compact Linear Collider (CLIC) Power Extraction and Transfer Structure (PETS) are presented.

  9. Join the CERN ISEF special award winners | 16 June - 3 p.m.

    2016-01-01

    Come and join the CERN ISEF special award winners at their lightning talks session on 16 June at 3.00 p.m. in the main auditorium.   The 2016 Intel ISEF CERN special award winners on stage with the selection committee on 17 May 2016 in Phoenix, Arizona, USA. (Picture: Society for Science and the Public) Between 11 and 17 June 2016, the ten finalists of the Intel International Science and Engineering Fair (ISEF) who won the CERN Special Award, will visit CERN to partake in various educational lectures. ISEF is the world's largest international pre-college science competition, with approximately 1,700 high school students from more than 75 countries taking part. They will present their projects in short 5 minutes lightning talks' sessions at the main auditorium on Thursday 16 June at 3 p.m. The award winners would be also very happy to have a chance to interact and discuss with you af...

  10. (2p2)3P state of H- and the convergence of the CI series

    A sequence of increasingly accurate configuration interaction (CI) wave functions is used to discuss binding and the convergence of the CI series for the (2p2)3P state of H-. We get an upper bound energy E/sub u/=-0.1 253 547 166 a.u.(H), the lowest yet obtained, lying within an estimated 0.4 μhartree from the exact eigenvalue of Schroedinger's nonrelativistic equation. The angular energy limits define angular energy increments ΔE/sub l/ which follows the formula ΔE/sub l/approx. =0.18 (l+1/2)-7a.u. for l> or =4. Five or eighty per cent of binding is obtained with a three-term Psi=c1(2p)2+c2(2p')2+c3 (3d)2 depending on whether the 2p,2p', and 3d orbitals are energy optimized STO's or accurate natural orbitals, respectively

  11. Half-quantized Non-Abelian Vortices in Neutron $^3P_2$ Superfluids inside Magnetars

    Masuda, Kota

    2016-01-01

    We point out that half-quantized non-Abelian vortices exist as the minimum energy states in rotating neutron $^3P_2$ superfluids in the inner cores of magnetars with magnetic field greater than $3 \\times 10^{15}$ Gauss, while they do not in ordinary neutron stars with smaller magnetic fields. One integer vortex is split into two half-quantized vortices. The number of vortices is about $10^{19}$ and they are separated at about $\\mu$m in a vortex lattice for typical parameters, while the vortex core size is about 10-100 fm. They are non-Abelian vortices characterized by non-Abelian first homotopy group, and consequently when two vortices corresponding to non-commutative elements collide, a rung vortex must be created between them, implying the formation of an entangled vortex network inside the cores of magnetars. We find the spontaneous magnetization in the vortex core showing anti-ferromagnetism whose typical magnitude is about $10^{8-9}$ Gauss that is ten times larger than that of integer vortices, when exte...

  12. ACE3P Computations of Wakefield Coupling in the CLIC Two-Beam Accelerator

    Candel, Arno; Li, Z.; Ng, C.; Rawat, V.; Schussman, G.; Ko, K.; /SLAC; Syratchev, I.; Grudiev, A.; Wuensch, W.; /CERN

    2010-10-27

    The Compact Linear Collider (CLIC) provides a path to a multi-TeV accelerator to explore the energy frontier of High Energy Physics. Its novel two-beam accelerator concept envisions rf power transfer to the accelerating structures from a separate high-current decelerator beam line consisting of power extraction and transfer structures (PETS). It is critical to numerically verify the fundamental and higher-order mode properties in and between the two beam lines with high accuracy and confidence. To solve these large-scale problems, SLAC's parallel finite element electromagnetic code suite ACE3P is employed. Using curvilinear conformal meshes and higher-order finite element vector basis functions, unprecedented accuracy and computational efficiency are achieved, enabling high-fidelity modeling of complex detuned structures such as the CLIC TD24 accelerating structure. In this paper, time-domain simulations of wakefield coupling effects in the combined system of PETS and the TD24 structures are presented. The results will help to identify potential issues and provide new insights on the design, leading to further improvements on the novel CLIC two-beam accelerator scheme.

  13. Utilising the `3P-model' to Characterise the Discipline of Didactics of Science

    Adúriz-Bravo, Agustín; Izquierdo-Aymerich, Mercè

    In our research within didactics of science, we have been exploring contributions of the so called cognitive models from contemporary philosophy of science. We have used these philosophical frameworks on different levels. As an outcome, we have formulated a model of didactics of science according to which this discipline adapts and transforms theoretical contributions from different scholarly fields. In this paper, we concentrate on this description of didactics of science, which we have called the 3P-model (i.e., philosophy + psychology + pedagogy). This model of the internal functioning of the discipline may be useful to make innovations in science curriculum design and re-conceptualise the role of science teachers as professionals. We see didactics of science as a set of interrelated activities, performed by different individuals, and ranging from theoretical production to practice of science education at school. We find the concept of technoscience suitable to account for this diversity of goals. According to this concept, scientific disciplines are identified both with generation of knowledge and with active intervention on the world. Within current didactics of science, we recognise several kinds of research, having goals more or less directed to practical intervention in science education.

  14. Precision frequency measurement of 1S0–3P1 intercombination lines of Sr isotopes

    We report on frequency measurement of the intercombination (5s2)1S0–(5s5p)3P1 transition of the four natural isotopes of strontium, including 88Sr (82.58%), 87Sr (7.0%), 86Sr (9.86%), and 84Sr (0.56%). A narrow-linewidth laser that is locked to an ultra-low expansion (ULE) optical cavity with a finesse of 12000 is evaluated at a linewidth of 200 Hz with a fractional frequency drift of 2.8×10−13 at an integration time of 1 s. The fluorescence collector and detector are specially designed, based on a thermal atomic beam. Using a double-pass acousto-optic modulator (AOM) combined with a fiber and laser power stabilization configuration to detune the laser frequency enables high signal-to-noise ratios and precision saturated spectra to be obtained for the six transition lines, which allows us to determine the transition frequency precisely. The optical frequency is measured using an optical frequency synthesizer referenced to an H maser. Both the statistical values and the final values, including the corrections and uncertainties, are derived for a comparison with the values given in other works. (paper)

  15. miR-193a-3p regulation of chemoradiation resistance in oesophageal cancer cells via the PSEN1 gene.

    Meng, Fang; Qian, Liting; Lv, Lei; Ding, Bojin; Zhou, Gieping; Cheng, Xu; Niu, Sanqiang; Liang, Yu

    2016-04-01

    Chemoradiation therapy is an important component of the curative treatment for oesophageal carcinomas. These therapeutic effects are prevented in patients according to radioresistance and multi-drug resistance, and the cause of such resistance remains unclear. In this study, we identified the role of miR-193a-3p in modulating the radioresistance and chemoresistance of oesophageal cancer cells. We found that KYSE150 and KYSE410 cells could be characterized as relatively radiation-sensitive and radiation-resistant cells, respectively. Similarly, KYSE150 and KYSE410 cells were found to be chemosensitive and chemoresistant, respectively. Over-expression of miR-193a-3p increased the radioresistance and chemoresistance of oesophageal squamous cell carcinoma (ESCC) cells. In contrast, the down-regulation of miR-193a-3p decreased the radioresistance and chemoresistance of ESCC cells. In addition, miR-193a-3p inducing DNA damage has also been demonstrated through measuring the level of gamma-H2AX associated with miR-193a-3p. Moreover, a small interfering RNA(siRNA)-induced repression of the PSEN1 gene had an effect similar to that of miR-193a-3p up-regulation. The above processes also inhibited oesophageal cancer cells apoptosis. These findings suggest that miR-193a-3p contributes to the radiation and chemotherapy resistance of oesophageal carcinoma by down-regulating PSEN1. Thus, miR-193a-3p and PSEN1 might be potential biomarkers for chemoradiation resistant cancers. PMID:26743123

  16. Prm3p Is a Pheromone-induced Peripheral Nuclear Envelope Protein Required for Yeast Nuclear Fusion

    Shen, Shu; Tobery, Cynthia E.; Rose, Mark D.

    2009-01-01

    Nuclear membrane fusion is the last step in the mating pathway of the yeast Saccharomyces cerevisiae. We adapted a bioinformatics approach to identify putative pheromone-induced membrane proteins potentially required for nuclear membrane fusion. One protein, Prm3p, was found to be required for nuclear membrane fusion; disruption of PRM3 caused a strong bilateral defect, in which nuclear congression was completed but fusion did not occur. Prm3p was localized to the nuclear envelope in pheromon...

  17. Electron impact excitation out of the metastable levels of argon into the 3p54p J = 3 level

    We have measured the direct cross section for electron impact excitation out of the metastable 3p54s[3/2]20 level (1s5 in Paschen's notation) into the 3p54p[5/2]3 level (2p9) of argon from threshold to 800 eV. The direct cross section is 40 x 10-16 cm2 at 10 eV. (author)

  18. MicroRNA-124-3p regulates cell proliferation, invasion, apoptosis, and bioenergetics by targeting PIM1 in astrocytoma.

    Deng, Danni; Wang, Lei; Chen, Yao; Li, Bowen; Xue, Lian; Shao, Naiyuan; Wang, Qiang; Xia, Xiwei; Yang, Yilin; Zhi, Feng

    2016-07-01

    The PIM1 protein is an important regulator of cell proliferation, the cell cycle, apoptosis, and metabolism in various human cancers. MicroRNAs (miRNAs) are powerful post-transcriptional gene regulators that function through translational repression or transcript destabilization. Therefore, we aimed to identify whether a close relationship exists between PIM1 and miRNAs. PIM1 protein levels and mRNA levels were significantly upregulated in astrocytoma tissues, indicating the oncogenic role of PIM1 in astrocytoma. Further bioinformatics analysis indicated that miR-124-3p targeted the 3'-UTR of PIM1. We also observed an inverse correlation between the miR-124-3p levels and PIM1 protein or mRNA levels in astrocytoma samples. Next, we experimentally confirmed that miR-124-3p directly recognizes the 3'-UTR of the PIM1 transcript and regulates PIM1 expression at both the protein and mRNA levels. Furthermore, we examined the biological consequences of miR-124-3p targeting PIM1 in vitro. We showed that the repression of PIM1 in astrocytoma cancer cells by miR-124-3p suppressed proliferation, invasion, and aerobic glycolysis and promoted apoptosis. We observed that the restoration or inhibition of PIM1 activity resulted in effects that were similar to those induced by miR-124-3p inhibitors or mimics in cancer cells. Finally, overexpression of PIM1 rescued the inhibitory effects of miR-124-3p. In summary, these findings aid in understanding the tumor-suppressive role of miR-124-3p in astrocytoma pathogenesis through the inhibition of PIM1 translation. PMID:27088547

  19. Novel Mad2-targeting miR-493-3p controls mitotic fidelity and cancer cells' sensitivity to paclitaxel.

    Tambe, Mahesh; Pruikkonen, Sofia; Mäki-Jouppila, Jenni; Chen, Ping; Elgaaen, Bente Vilming; Straume, Anne Hege; Huhtinen, Kaisa; Cárpen, Olli; Lønning, Per Eystein; Davidson, Ben; Hautaniemi, Sampsa; Kallio, Marko J

    2016-03-15

    The molecular pathways that contribute to the proliferation and drug response of cancer cells are highly complex and currently insufficiently characterized. We have identified a previously unknown microRNA-based mechanism that provides cancer cells means to stimulate tumorigenesis via increased genomic instability and, at the same time, evade the action of clinically utilized microtubule drugs. We demonstrate miR-493-3p to be a novel negative regulator of mitotic arrest deficient-2 (MAD2), an essential component of the spindle assembly checkpoint that monitors the fidelity of chromosome segregation. The microRNA targets the 3' UTR of Mad2 mRNA thereby preventing translation of the Mad2 protein. In cancer cells, overexpression of miR-493-3p induced a premature mitotic exit that led to increased frequency of aneuploidy and cellular senescence in the progeny cells. Importantly, excess of the miR-493-3p conferred resistance of cancer cells to microtubule drugs. In human neoplasms, miR-493-3p and Mad2 expression alterations correlated with advanced ovarian cancer forms and high miR-493-3p levels were associated with reduced survival of ovarian and breast cancer patients with aggressive tumors, especially in the paclitaxel therapy arm. Our results suggest that intratumoral profiling of miR-493-3p and Mad2 levels can have diagnostic value in predicting the efficacy of taxane chemotherapy. PMID:26943585

  20. Integrated multi-omics analyses reveal the pleiotropic nature of the control of gene expression by Puf3p.

    Kershaw, Christopher J; Costello, Joseph L; Talavera, David; Rowe, William; Castelli, Lydia M; Sims, Paul F G; Grant, Christopher M; Ashe, Mark P; Hubbard, Simon J; Pavitt, Graham D

    2015-01-01

    The PUF family of RNA-binding proteins regulate gene expression post-transcriptionally. Saccharomyces cerevisiae Puf3p is characterised as binding nuclear-encoded mRNAs specifying mitochondrial proteins. Extensive studies of its regulation of COX17 demonstrate its role in mRNA decay. Using integrated genome-wide approaches we define an expanded set of Puf3p target mRNAs and quantitatively assessed the global impact of loss of PUF3 on gene expression using mRNA and polysome profiling and quantitative proteomics. In agreement with prior studies, our sequencing of affinity-purified Puf3-TAP associated mRNAs (RIP-seq) identified mRNAs encoding mitochondrially-targeted proteins. Additionally, we also found 720 new mRNA targets that predominantly encode proteins that enter the nucleus. Comparing transcript levels in wild-type and puf3∆ cells revealed that only a small fraction of mRNA levels alter, suggesting Puf3p determines mRNA stability for only a limited subset of its target mRNAs. Finally, proteomic and translatomic studies suggest that loss of Puf3p has widespread, but modest, impact on mRNA translation. Taken together our integrated multi-omics data point to multiple classes of Puf3p targets, which display coherent post-transcriptional regulatory properties and suggest Puf3p plays a broad, but nuanced, role in the fine-tuning of gene expression. PMID:26493364

  1. Up- regulation of miR-328-3p sensitizes non-small cell lung cancer to radiotherapy.

    Ma, Wei; Ma, Chao-Nan; Zhou, Nan-Nan; Li, Xian-Dong; Zhang, Yi-Jie

    2016-01-01

    MicroRNAs (miRNAs) are believed to be resistant against radiotherapy in certain types of cancers. The aim of our study was to determine the clinical application of miRNAs in non-small cell lung cancer (NSCLC). Sixty NSCLC tissue samples and adjacent histologically normal tissues were obtained for miRNAs microarray analysis and validated by RT-qPCR. Correlation between miRNA expression level and clinicopathological features was evaluated. Our study examined the influence of changed miRNA expression on the damaged DNA and its associated radio sensitivity. Luciferase assay was performed to determine potential effects on the targeted gene. Our study identified fifteen altered miRNAs in which miR-328-3p was down regulated in NSCLC tumour tissue as compared to normal tissues. Down-expression of miR-328-3p was positively associated with an enhanced lymph node metastasis, advanced clinical stage and a shortened survival rate. miR-328-3p expression was decreased in A549 cells compared to other NSCLC cell lines. Up-regulation of miR-328-3p demonstrated a survival inhibition effect in A549 and restored NSCLC cells' sensitivity to radio therapy. An increased miR-328-3p expression promoted irradiation-induced DNA damage in cells. γ-H2AX was identified as the direct target of miR-328-3p. Over-expressed miR-328-3p can improve the radiosensitvity of cells by altering the DNA damage/repair signalling pathways in NSCLC. PMID:27530148

  2. Colloidal CdSe/Cu3P/CdSe nanocrystal heterostructures and their evolution upon thermal annealing.

    De Trizio, Luca; De Donato, Francesco; Casu, Alberto; Genovese, Alessandro; Falqui, Andrea; Povia, Mauro; Manna, Liberato

    2013-05-28

    We report the synthesis of colloidal CdSe/Cu(3)P/CdSe nanocrystal heterostructures grown from hexagonal Cu(3)P platelets as templates. One type of heterostructure was a sort of "coral", formed by vertical pillars of CdSe grown preferentially on both basal facets of a Cu(3)P platelet and at its edges. Another type of heterostructure had a "sandwich" type of architecture, formed by two thick, epitaxial CdSe layers encasing the original Cu(3)P platelet. When the sandwiches were annealed under vacuum up to 450 °C, sublimation of P and Cd species with concomitant interdiffusion of Cu and Se species was observed by in situ HR- and EFTEM analyses. These processes transformed the starting sandwiches into Cu2Se nanoplatelets. Under the same conditions, both the pristine (uncoated) Cu(3)P platelets and a control sample made of isolated CdSe nanocrystals were stable. Therefore, the thermal instability of the sandwiches under vacuum might be explained by the diffusion of Cu species from Cu(3)P cores into CdSe domains, which triggered sublimation of Cd, as well as out-diffusion of P species and their partial sublimation, together with the overall transformation of the sandwiches into Cu(2)Se nanocrystals. A similar fate was followed by the coral-like structures. These CdSe/Cu(3)P/CdSe nanocrystals are therefore an example of a nanostructure that is thermally unstable, despite its separate components showing to be stable under the same conditions. PMID:23557168

  3. The state dependence of the interaction of metastable rare gas atoms Rg sup * (ms sup 3 P sub 2 , sup 3 P sub 0 ) (Rg=Ne, Ar, Kr, Xe) with ground state sodium atoms

    Schohl, S.; Mueller, M.W.; Meijer, H.A.J.; Ruf, M.W.; Hotop, H. (Kaiserslautern Univ. (Germany, F.R.). Fachbereich Physik); Morgner, H. (Witten-Herdecke Univ., Witten (Germany, F.R.). Inst. fuer Experimentalphysik)

    1990-08-01

    Using crossed beams of metastable rare gas atoms Rg{sup *}(ms {sup 3}P{sub 2}, {sup 3}P{sub 0}) (Rg=Ne, Ar, Kr, Xe) and ground state sodium atoms Na(3s {sup 2}S{sub 1/2}), we have measured the energy spectra of electrons released in the respective Penning ionization processes at thermal collision energies. For Rg{sup *}({sup 3}P{sub 2})+Na(3s), the spectra are quite similar for the different rare gases, both in width and shape; they reflect attractive interactions in the entrance channel with well depths D{sub e}{sup *} (meV) decreasing slowly from Rg=Ne to Xe as follows: 676(18); 602(23); 565(26); 555(30). For Rg{sup *}({sup 3}P{sub 0})+Na(3 s), the spectra vary strongly with the rare gas, indicating a change in the character of the interaction from van der Waals type attraction (Ne) to chemical binding for Kr and Xe with well depths D{sub e}{sup *} (meV) of: 51(19); 107(25); 432(30); 530(50). These findings are explained through model calculations of the respective potential curves, in which the exchange and the spin orbit interaction in the excited rare gas and the molecular interaction between the two valence s-electrons in terms of suitably chosen singlet and triplet potentials are taken into account. These calculations also explain qualitatively the experimental finding that the ratios q{sub 2}/q{sub 0} of the ionization cross sections for Rg{sup *}({sup 3}P{sub 2})+Na and Rg{sup *}({sup 3}P{sub 0})+Na vary strongly with the rare gas from Ne to Xe as follows: 15.8(3.2); 2.6(4); 1.4(2); 1.6(4). (orig.).

  4. Physico-chemical studies of MgNa3P3O10·12H2O

    Fahim I.

    2013-09-01

    Full Text Available A magnesium-sodium triphosphate MgNa3P3O10·12H2O already known has been prepared by the method of ion exchange resin of aqueous chemistry and studied by X-Ray diffraction, thermal analyses (TGA-DTA, differential scanning calorimetry (DSC and infrared spectrometry which show the characteristic bands of a triphosphate P3O. The results of differential thermal analysis, X-Ray powder diffraction and IR spectra of the compound heated at different temperatures showed that, after dehydration, MgNa3P3O10·12H2O decomposes into an amorphous compound, then it crystallizes at 600 ° C in order to give the anhydrous triphosphate MgNa3P3O10. MgNa3P3O10 is stable until its melting point at 612 ° C. Two different methods Ozawa and KAS have been selected in studying the kinetics of thermal behavior of the triphosphate P3O10 for the first time. The kinetic and thermodynamic characteristics of the dehydration of MgNa3P3O10·12H2O and the thermal phenomena accompanying this dehydration were determinated and discussed on the basis of the proposed crystalline structure.

  5. MicroRNA-29a-3p attenuates ET-1-induced hypertrophic responses in H9c2 cardiomyocytes.

    Li, Man; Wang, Nan; Zhang, Jian; He, Hong-Peng; Gong, Hui-Qin; Zhang, Rui; Song, Tie-Feng; Zhang, Li-Nan; Guo, Zhi-Xia; Cao, Dong-Sun; Zhang, Tong-Cun

    2016-07-01

    Transcription factor nuclear factor of activated T cells c4 (NFATc4) is the best-characterized target for the development of cardiac hypertrophy. Aberrant microRNA-29 (miR-29) expression is involved in the development of cardiac fibrosis and congestive heart failure. However, whether miR-29 regulates hypertrophic processes is still not clear. In this study, we investigated the potential functions of miR-29a-3p in endothelin-1 (ET-1)-induced cardiomyocyte hypertrophy. We showed that miR-29a-3p was down-regulated in ET-1-treated H9c2 cardiomyocytes. Overexpression of miR-29a-3p significantly reduced ET-1-induced hypertrophic responses in H9c2 cardiomyocytes, which was accompanied by a decrease in NFATc4 expression. miR-29a-3p targeted directly to the 3'-UTR of NFATc4 mRNA and silenced NFATc4 expression. Our results indicate that miR-29a-3p inhibits ET-1-induced cardiomyocyte hypertrophy via inhibiting NFATc4 expression. PMID:26992639

  6. Soft-mode enhanced superconductivity in the antiperovskite APt{sub 3}P, A=Sr, Ca, La

    Heid, Rolf; Bohnen, Klaus-Peter [Institut fuer Festkoerperphysik, Karlsruher Institut fuer Technologie (Germany)

    2013-07-01

    The surprising discovery of superconductivity in iron pnictides initiated an increased search for superconductivity in other unconventional compounds. Recently, a new class of P-based antiperovskites was synthesized, and superconductivity with a T{sub c} of 8.4 K was observed for SrPt{sub 3}P. Specific heat measurements indicated a strong-coupling scenario and the presence of low-energy phonon modes. Replacing Sr by Ca or La resulted in lower T{sub c}'s and a progressive loss of strong-coupling signatures. Here we present results of a first principles study of lattice dynamics and electron-phonon coupling properties of APt{sub 3}P, A=Sr,Ca,La, with full consideration of spin-orbit interaction. For SrPt{sub 3}P, the coupling is carried almost totally by a single low-frequency branch consisting of planar Pt vibrations, which result in a coupling constant of λ∼2. In CaPt{sub 3}P this mode stiffens significantly thereby reducing λ to about 1, while in LaPt{sub 3}P the coupling is further reduced to 0.5 because of a shift of the electronic bands. In contrast to previous work, our ab initio results provide a consistent quantitative description of the key features of superconductivity in this new class of materials within the framework of the strong-coupling Eliashberg theory.

  7. Development of a chromosomally integrated metabolite-inducible Leu3p-alpha-IPM "off-on" gene switch.

    Maria Poulou

    Full Text Available BACKGROUND: Present technology uses mostly chimeric proteins as regulators and hormones or antibiotics as signals to induce spatial and temporal gene expression. METHODOLOGY/PRINCIPAL FINDINGS: Here, we show that a chromosomally integrated yeast 'Leu3p-alpha-IotaRhoMu' system constitutes a ligand-inducible regulatory "off-on" genetic switch with an extensively dynamic action area. We find that Leu3p acts as an active transcriptional repressor in the absence and as an activator in the presence of alpha-isopropylmalate (alpha-IotaRhoMu in primary fibroblasts isolated from double transgenic mouse embryos bearing ubiquitously expressing Leu3p and a Leu3p regulated GFP reporter. In the absence of the branched amino acid biosynthetic pathway in animals, metabolically stable alpha-IPM presents an EC(50 equal to 0.8837 mM and fast "OFF-ON" kinetics (t(50ON = 43 min, t(50OFF = 2.18 h, it enters the cells via passive diffusion, while it is non-toxic to mammalian cells and to fertilized mouse eggs cultured ex vivo. CONCLUSIONS/SIGNIFICANCE: Our results demonstrate that the 'Leu3p-alpha-IotaRhoMu' constitutes a simpler and safer system for inducible gene expression in biomedical applications.

  8. Reactions of N+ (3P) ions with H2 and HD molecules at low temperatures

    Grozdanov, Tasko P.; McCarroll, Ronald; Roueff, Evelyne

    2016-04-01

    formation of ND+. The calculated value is consistent with the available experimental data. Conclusions: The present results allow for the determination of reaction rate coefficients for any given distribution of specific fine structure and rotational state populations of the reactants. In interstellar conditions, where N+ is in its 3P0 state and para- and ortho-H2 respectively in J = 0 and J = 1. Our results enable a study of the influence of the ortho/para evolution of molecular hydrogen on the formation of nitrogen compounds.

  9. Regulatory role of the sequences downstream from nodD3 P1 promoter of Rhizobium meliloti

    2000-01-01

    The 660 bp region between nodD3 P1 promoter and the following coding region of Rhizobium meliloti has been studied.This region is designated "downstream sequences".It consists of two potential open reading frames,ORF1 and ORF2.Studies on the role of the downstream sequences on the activity of nodD3 P1 with nod D3(P1)-lacZ fusion show that deletion of the sequences containing ORF2 causes the increase of the activity of the fusion; on the contrary,addition of extra copies of ORF2 markedly decreases the activity of the fusion.These results indicate that the product of ORF2 plays a negative role in the expression of nod D3.

  10. Studies of pH regulation by Btn1p, the yeast homolog of human Cln3p.

    Pearce, D A; Nosel, S A; Sherman, F

    1999-04-01

    Although the gene responsible for Batten disease, CLN3, was positionally cloned in 1995, the function of Cln3p and the molecular basis of the disease still remain elusive. We previously reported that the yeast Saccharomyces cerevisiae contains a homolog to Cln3p, designated Btn1p, and that the human Cln3p complemented the pH-dependent resistance to D-(-)-threo-2-amino-1-[p-nitrophenyl]-1, 3-propanediol in btn1-Delta yeast mutants. We have determined that yeast lacking Btn1p have an elevated ability to acidify media during growth that correlates with an elevated plasma membrane ATPase activity. Btn1p may be involved in maintaining pH homeostasis of yeast cells. PMID:10191121

  11. Biomimetic-Inspired Infrared Sensors from Zn3P2 Microwires: Study of Their Photoconductivity and Infrared Spectrum Properties

    M. Israelowitz

    2014-01-01

    Full Text Available The fire beetle, Melanophila acuminata (Coleoptera: Buprestidae, senses infrared radiation at wavelengths of 3 and 10–25 microns via specialized protein-containing sensilla. Although the protein denatures outside of a biological system, this detection mechanism has inspired our bottom-up approach to produce single zinc phosphide microwires via vapour transport for IR sensing. The Zn3P2 microwires were immobilized and electrical contact was made by dielectrophoresis. Photoconductivity measurements have been extended to the near IR range, spanning the Zn3P2 band gaps. Purity and integrity of the Zn3P2 microwires including infrared light scattering properties were confirmed by infrared transmission microscopy. This biomimetic microwire shows promise for infrared chip development.

  12. Doubly excited {sup 3}P{sup e} resonance states of two-electron positive ions in Debye plasmas

    Hu, Xiao-Qing; Wang, Yang; Kar, Sabyasachi, E-mail: skar@hit.edu.cn, E-mail: karsabyasachi@yahoo.com [Center for Theoretical Atomic and Molecular Physics, Academy of Fundamental and Interdisciplinary Sciences, Harbin Institute of Technology, Harbin 150080 (China); Jiang, Zishi [College of Physical Science and Technology, Heilongjiang University, Harbin 150080 (China); Jiang, Pinghui [College of Electrical and Information Engineering, Heilongjiang Institute of Technology, Harbin 150050 (China)

    2015-11-15

    We investigate the doubly excited {sup 3}P{sup e} resonance states of two-electron positive ions Li{sup +}, Be{sup 2+}, B{sup 3+}, and C{sup 4+} by employing correlated exponential wave functions. In the framework of the stabilization method, we calculate two series (3pnp and 3dnd) of {sup 3}P{sup e} resonances below the N = 3 threshold. The {sup 3}P{sup e} resonance parameters (resonance energies and widths) are reported for the first time as a function of the screening parameter. For free-atomic cases, comparisons are made with the reported results and few resonance states are reported for the first time.

  13. Na*(3p)-Formation under grazing scattering of Na[sup +]-ions at an Al(111) surface

    Zimny, R. (Inst. fuer Kernphysik, Univ. Muenster (Germany)); Borisov, A.G. (Dept. of Physics, Moscow State Univ. (Russian Federation))

    1994-06-01

    Excited Na*(3p)-atoms are observed in grazing surface-collision experiments with Na[sup +]-beams. Such atoms can be formed beyond a certain threshold velocity via resonant electron transfer between atomic and metallic conduction band levels due to motion of the atom relative to the surface of the metal (''kinematic resonance''). This mechanism is studied here theoretically employing two different techniques: the nonperturbative ''Coupled Angular Mode'' (CAM) method and the approximate ''Transfer Hamiltonian'' (TH) method. The calculated Na*(3p)-populations agree well with recent experimental results. Moreover, the complete density matrix of the Na*(3p)-subspace has been computed with the TH-method for ion-energies between 10 and 300 keV. (orig.)

  14. BOT3P: a mesh generation software package for transport analysis with deterministic and Monte Carlo codes

    BOT3P consists of a set of standard Fortran 77 language programs that gives the users of the deterministic transport codes DORT, TORT, TWODANT, THREEDANT, PARTISN and the sensitivity code SUSD3D some useful diagnostic tools to prepare and check the geometry of their input data files for both Cartesian and cylindrical geometries, including graphical display modules. Users can produce the geometrical and material distribution data for all the cited codes for both two-dimensional and three-dimensional applications and, only in 3-dimensional Cartesian geometry, for the Monte Carlo Transport Code MCNP, starting from the same BOT3P input. Moreover, BOT3P stores the fine mesh arrays and the material zone map in a binary file, the content of which can be easily interfaced to any deterministic and Monte Carlo transport code. This makes it possible to compare directly for the same geometry the effects stemming from the use of different data libraries and solution approaches on transport analysis results. BOT3P Version 5.0 lets users optionally and with the desired precision compute the area/volume error of material zones with respect to the theoretical values, if any, because of the stair-cased representation of the geometry, and automatically update material densities on the whole zone domains to conserve masses. A local (per mesh) density correction approach is also available. BOT3P is designed to run on Linux/UNIX platforms and is publicly available from the Organization for Economic Cooperation and Development (OECD/NEA)/Nuclear Energy Agency Data Bank. Through the use of BOT3P, radiation transport problems with complex 3-dimensional geometrical structures can be modelled easily, as a relatively small amount of engineer-time is required and refinement is achieved by changing few parameters. This tool is useful for solving very large challenging problems, as successfully demonstrated not only in some complex neutron shielding and criticality benchmarks but also in a power

  15. Lifetime measurement of the metastable 2{sup 3}P{sub 0} state in He-like uranium

    Toleikis, S. [Lawrence Berkeley National Laboratory, 1 Cyclotron Rd., MS 88R0192, Berkeley, CA 94720-8101 (United States) and Texas A and M University, College Station, TX (United States)]. E-mail: stoleikis@lbl.gov; Manil, B. [GANIL, Caen (France); Bednarz, G. [IFUJ, Cracow (Poland); Berdermann, E. [GSI, Darmstadt (Germany); Beyer, H.F. [GSI, Darmstadt (Germany); Bosch, F. [GSI, Darmstadt (Germany); Braeuning-Demian, A. [GSI, Darmstadt (Germany); Gumberidze, A. [GSI, Darmstadt (Germany); Indelicato, P. [Universite P. et M. Curie, Paris (France); Kozhuharov, C. [GSI, Darmstadt (Germany); Liesen, D. [GSI, Darmstadt (Germany); Marrus, R. [University of California, Berkeley, CA (United States); Mokler, P.H. [GSI, Darmstadt (Germany); Stachura, Z. [INP, Cracow (Poland); Stoehlker, T. [GSI, Darmstadt (Germany); Warczak, A. [IFUJ, Cracow (Poland)

    2005-07-01

    The lifetime of the 2{sup 3}P{sub 0} state in He-like uranium has been measured in a beam-foil time-of-flight experiment at the Gesellschaft fur Schwerionenforschung accelerator facility with the result {tau}(2{sup 3}P{sub 0})=58.2(9.5)ps. With the measured lifetime it is possible to derive a value of {delta}E{sub 2s}{sup Lamb}=76.3+/-20.6eV for the n=2 Lamb shift in uranium.

  16. Multi-tiered genomic analysis of head and neck cancer ties TP53 mutation to 3p loss

    Gross, Andrew M.; Ryan K. Orosco; Shen, John P.; Egloff, Ann Marie; Carter, Hannah; Hofree, Matan; Choueiri, Michel; Charles S. Coffey; Lippman, Scott M.; Hayes, D. Neil; Cohen, Ezra E.; Grandis, Jennifer R.; Nguyen, Quyen T.; Ideker, Trey

    2014-01-01

    Head and neck squamous cell carcinoma (HNSCC) is characterized by aggressive behavior with a propensity for metastasis and recurrence. Here we report a comprehensive analysis of the molecular and clinical features of HNSCC that govern patient survival. We find that TP53 mutation is frequently accompanied by loss of chromosome 3p, and that the combination of both events associates with a surprising decrease in survival rates (1.9 years versus >5 years for TP53 mutation alone). The TP53-3p inte...

  17. Spectroscopy of 1S0- 3P1 transition of magnesium atom in an external absorption cell

    Bagayev, S. N.; Baraulya, V. I.; Bonert, A. E.; Goncharov, A. N.; Seydaliev, M. R.

    2001-09-01

    The results of saturated absorption spectroscopy of the intercombination 1S0- 3P1 transition of magnesium atoms at 457 nm in an external absorption cell are presented. A laser system based on a Ti:Sa laser with frequency doubling in a LBO nonlinear crystal was used in these experiments. Saturated absorption resonances of magnesium in an external cell at the 1S0- 3P1 transition have been obtained for the first time. Pressure broadening of resonances equal to 12.5±1.5 kHz/mTorr has been measured.

  18. 'Oxygen' effect in ionization loss spectrum of Cu3p in YBa2Cu3Ox ceramics

    YBaCu3Ox ceramic samples with different oxygen concentration (6.2; 6.5 and 6.9) in a volume are investigated. The process of degradation of samples with high X in the vacuum is analyzed. The surface purification is performed through scribing in argon atmosphere. A new effect is ascertained which is manifested in high sensitivity of Cu3p ionization loss spectrum to the density of the non-occupied electron states at the Fermi level. It is shown that Cu3p ionization spectrum can serve as a new criterion of superconductor surface quality from the viewpoint of its electronic structure

  19. High lying energy positions of doubly (2pns) {sup 1,3}P{sup o} and (2pnd) {sup 1,3}P{sup o} excited states of the beryllium atom

    Sakho, I., E-mail: aminafatima_sakho@yahoo.fr [UFR Sciences and Technologies, Department of physics, University of Ziguinchor, Ziguinchor (Senegal)

    2011-12-15

    The Screening Constant by Unit Nuclear Charge (SCUNC) method is used to study (2pns) {sup 1,3}P{sup o} and (2pnd) {sup 1,3}P{sup o} autoionizing states of the beryllium atom. Energy positions are reported up to n=20. In addition, resonance widths of the (2pns) {sup 1}P{sup o} states also presented. The current results compared very well to available theoretical and experimental literature values up to n=15. The accurate data presented in this work may be of interest for future experimental and theoretical studies in the photoabsorption spectrum of Be. - Highlights: > Accurate energy positions of (2pns) {sup 1,3}P{sup o} and (2pnd) {sup 1,3}P{sup o} (n=3-20) autoionizing states of Be atoms. > Currently results compared very well to theoretical and experimental literature values up to n=15. > Presently data may be of interest for future experimental and theoretical studies in the photoabsorption spectrum of Be.

  20. Experimental transition probabilities of the intercombination line 1s2ws21S0-1s22s3p3P01 in Be-like ions of Na through Si

    The decay of the 1s22s3p3 Po0,1,2 levels in Be-like ions of Na, Mg, Al and Si has been investigated using the beam-foil method. The mean wavelengths of the transition 1s22p23P0,1,2-1s22s3p 3Po0,1,2 and the lifetimes of the 1s22s3p 3Po0,1,2 levels have been measured. An intercomparison of these lifetimes yields the transition probability of the intercombination line 1s22s21S0-1s22s3p 3Po1. The results are (3.45 ± 0.73) 109 s-1 in Na VIII, (5.55 ± 0.96) 109 s-1 in Mg IX, (1.21 ± 0.24) 1010 s-1 in Al X and (2.50 ± 0.54) 1010 s-1 in Si XI. (orig.)

  1. Radiative rates for E1, E2, M1, and M2 transitions among the 3s$^2$3p$^5$, 3s3p$^6$, and 3s$^2$3p$^4$3d configurations of Cl-like W LVIII

    Aggarwal, K M

    2014-01-01

    We report calculations of energy levels, radiative decay rates, and lifetimes for transitions among the 3s$^2$3p$^5$, 3s3p$^6$, and 3s$^2$3p$^4$3d configurations of Cl-like W LVIII. The general-purpose relativistic atomic structure package (GRASP) has been adopted for our calculations. Comparisons are made with the most recent results of Mohan et al. [Can. J. Phys. {\\bf 92} (2014) xxx] and discrepancies in lifetimes are noted, up to four orders of magnitude in some instances. Our energy levels are estimated to be accurate to better than 0.5\\%, whereas results for radiative rates and lifetimes should be accurate to better than 20\\%.

  2. Relativistic calculations of 3s2 1S0-3s3p 1P1 and 3s2 1S0-3s3p 3P1,2 transition probabilities in the Mg isoelectronic sequence

    Cheng Cheng; Gao Xiang; Qing Bo; Zhang Xiao-Le; Li Jia-Ming

    2011-01-01

    Using the multi-configuration Dirac-Fock self-consistent field method and the relativistic configuration-interaction method, calculations of transition energies, oscillator strengths and rates are performed for the 3s2 1S0-3s3p 1P1 spinallowed transition, 3s2 1S0-3s3p 3P1,2 intercombination and magnetic quadrupole transition in the Mg isoelectronic sequence (Mg Ⅰ, Al Ⅱ, Si ⅢⅢ, P Ⅳ and S Ⅴ). Electron correlations are treated adequately, including intravalence electron correlations. The influence of the Breit interaction on oscillator strengths and transition energies are investigated. Quantum electrodynamics corrections are added as corrections. The calculation results are found to be in good agreement with the experimental data and other theoretical calculations.

  3. Amyloid-β Peptide Aβ3pE-42 Induces Lipid Peroxidation, Membrane Permeabilization, and Calcium Influx in Neurons.

    Gunn, Adam P; Wong, Bruce X; Johanssen, Timothy; Griffith, James C; Masters, Colin L; Bush, Ashley I; Barnham, Kevin J; Duce, James A; Cherny, Robert A

    2016-03-18

    Pyroglutamate-modified amyloid-β (pE-Aβ) is a highly neurotoxic amyloid-β (Aβ) isoform and is enriched in the brains of individuals with Alzheimer disease compared with healthy aged controls. Pyroglutamate formation increases the rate of Aβ oligomerization and alters the interactions of Aβ with Cu(2+) and lipids; however, a link between these properties and the toxicity of pE-Aβ peptides has not been established. We report here that Aβ3pE-42 has an enhanced capacity to cause lipid peroxidation in primary cortical mouse neurons compared with the full-length isoform (Aβ(1-42)). In contrast, Aβ(1-42) caused a significant elevation in cytosolic reactive oxygen species, whereas Aβ3pE-42 did not. We also report that Aβ3pE-42 preferentially associates with neuronal membranes and triggers Ca(2+) influx that can be partially blocked by the N-methyl-d-aspartate receptor antagonist MK-801. Aβ3pE-42 further caused a loss of plasma membrane integrity and remained bound to neurons at significantly higher levels than Aβ(1-42) over extended incubations. Pyroglutamate formation was additionally found to increase the relative efficiency of Aβ-dityrosine oligomer formation mediated by copper-redox cycling. PMID:26697885

  4. Absence of mutations in the coding sequence of the potential tumor suppressor 3pK in metastatic melanoma

    Houben Roland

    2005-12-01

    Full Text Available Abstract Background Activation of Ras or Raf contributes to tumorigenesis of melanoma. However, constitutive Raf activation is also a characteristic of the majority of benign melanocytic nevi and high intensity signaling of either Ras or Raf was found to induce growth inhibition and senescence rather than transformation. Since the chromosome 3p kinase (3pK is a target of the Ras/Raf/Mek/Erk signaling pathway which antagonizes the function of the oncogene and anti-differentiation factor Bmi-1, 3pK may function as a tumor suppressor in tumors with constitutive Ras/Raf activation. Consequently, we tested whether inactivating 3pK mutations are present in melanoma. Methods 30 metastatic melanoma samples, which were positive for activating mutations of either BRaf or NRas, were analyzed for possible mutations in the 3pk gene. The 10 coding exons and their flanking intron sequences were amplified by PCR and direct sequencing of the PCR products was performed. Results This analysis revealed that besides the presence of some single nucleotide polymorphisms in the 3pk gene, we could not detect any possible loss of function mutation in any of these 30 metastatic melanoma samples selected for the presence of activating mutations within the Ras/Raf/Mek/Erk signaling pathway. Conclusion Hence, in melanoma with constitutively active Ras/Raf inactivating mutations within the 3pk gene do not contribute to the oncogenic phenotype of this highly malignant tumor.

  5. LEAN MANUFACTURING: CONTINUOUS IMPROVEMENT OF PRODUCTIVITY USING KAIZEN 3P PHILOSOPHY IN A LARGE SIZED COMPANY IN BOTUCATU, SP, BRAZIL

    Fernando Doriguel

    2015-06-01

    Full Text Available The search for excellence in a company´s production occurs mainly from developing reputable philosophies by training and monitoring groups which are formed by the company itself. Due to such factors as well as the necessity to increase excellence in production, new methodologies as Kaizen 3P (Production Preparation Process is still little known. This paper aims at deploying Lean Manufacturing which used Kaizen 3P method in a production line in order to reduce the operator time in production as wellas to reduce the product cycling time in assembling line. Results showed that Kaizen 3P method used in the company reduced man hour (MH in all the process reduction 660 hours. Furthermore assembling line had a time reduction 200 hours. Product route had a reduction of 17,6 kilometers. It was possible to conclude that the correct use of Kaizen 3P tool based on Lean manufacturing showed significant and productive gains when compared to old working methods adopted by the company.

  6. 78 FR 12130 - Social Security Ruling, SSR 13-3p; Appeal of an Initial Medical Disability Cessation...

    2013-02-21

    ... Social Security--Disability Insurance; 96.004 Social Security--Survivors Insurance; 96.006 Supplemental... From the Federal Register Online via the Government Publishing Office SOCIAL SECURITY ADMINISTRATION Social Security Ruling, SSR 13-3p; Appeal of an Initial Medical Disability Cessation...

  7. Reduced miR-659-3p Levels Correlate with Progranulin Increase in Hypoxic Conditions: Implications for Frontotemporal Dementia.

    Piscopo, Paola; Grasso, Margherita; Fontana, Francesca; Crestini, Alessio; Puopolo, Maria; Del Vescovo, Valerio; Venerosi, Aldina; Calamandrei, Gemma; Vencken, Sebastian F; Greene, Catherine M; Confaloni, Annamaria; Denti, Michela A

    2016-01-01

    Progranulin (PGRN) is a secreted protein expressed ubiquitously throughout the body, including the brain, where it localizes in neurons and is activated microglia. Loss-of-function mutations in the GRN gene are an important cause of familial frontotemporal lobar degeneration (FTLD). PGRN has a neurotrophic and anti-inflammatory activity, and it is neuroprotective in several injury conditions, such as oxygen or glucose deprivation, oxidative injury, and hypoxic stress. Indeed, we have previously demonstrated that hypoxia induces the up-regulation of GRN transcripts. Several studies have shown microRNAs (miRNAs) involvement in hypoxia. Moreover, in FTLD patients with a genetic variant of GRN (rs5848), the reinforcement of miR-659-3p binding site has been suggested to be a risk factor. Here, we report that miR-659-3p interacts directly with GRN 3'UTR as shown by luciferase assay in HeLa cells and ELISA and Western Blot analysis in HeLa and Kelly cells. Moreover, we demonstrate the physical binding between GRN mRNA and miR-659-3p employing a miRNA capture-affinity technology in SK-N-BE and Kelly cells. In order to study miRNAs involvement in hypoxia-mediated up-regulation of GRN, we evaluated miR-659-3p levels in SK-N-BE cells after 24 h of hypoxic treatment, finding them inversely correlated to GRN transcripts. Furthermore, we analyzed an animal model of asphyxia, finding that GRN mRNA levels increased at post-natal day (pnd) 1 and pnd 4 in rat cortices subjected to asphyxia in comparison to control rats and miR-659-3p decreased at pnd 4 just when GRN reached the highest levels. Our results demonstrate the interaction between miR-659-3p and GRN transcript and the involvement of miR-659-3p in GRN up-regulation mediated by hypoxic/ischemic insults. PMID:27199656

  8. Role of miR-222-3p in c-Src-Mediated Regulation of Osteoclastogenesis

    Shinya Takigawa

    2016-02-01

    Full Text Available MicroRNAs (miRNAs are small non-coding RNAs that play a mostly post-transcriptional regulatory role in gene expression. Using RAW264.7 pre-osteoclast cells and genome-wide expression analysis, we identified a set of miRNAs that are involved in osteoclastogenesis. Based on in silico analysis, we specifically focused on miR-222-3p and evaluated its role in osteoclastogenesis. The results show that the inhibitor of miR-222-3p upregulated the mRNA levels of nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1 and tartrate-resistant acid phosphatase (TRAP, while its mimicking agent downregulated their mRNA levels. Western blot analysis showed that its inhibitor increased the protein levels of TRAP and cathepsin K, while its mimicking agent decreased their levels. Genome-wide mRNA expression analysis in the presence and absence of receptor activator of nuclear factor κ-B ligand (RANKL predicted c-Src as a potential regulatory target of miR-222-3p. Live cell imaging using a fluorescence resonance energy transfer (FRET technique revealed that miR-222-3p acted as an inhibitor of c-Src activity, and a partial silencing of c-Src suppressed RANKL-induced expression of TRAP and cathepsin K, as well as the number of multi-nucleated osteoclasts and their pit formation. Collectively, the study herein demonstrates that miR-222-3p serves as an inhibitor of osteoclastogenesis and c-Src mediates its inhibition of cathepsin K and TRAP.

  9. Circulating miR-221-3p as a novel marker for early prediction of acute myocardial infarction.

    Coskunpinar, Ender; Cakmak, Huseyin Altug; Kalkan, Ali Kemal; Tiryakioglu, Necip Ozan; Erturk, Mehmet; Ongen, Zeki

    2016-10-10

    Recent studies have reported circulating microRNAs (miRNAs) as novel biomarkers for cardiovascular diseases including acute myocardial infarction, heart failure, diabetes mellitus, stroke, and acute pulmonary embolism. The aims of this study were 1) to compare the plasma expression levels of miRNAs in patients with acute coronary syndrome (ACS) and control subjects and in ST-elevation myocardial infarction (STEMI) and non-STEMI 2) to evaluate miRNAs potential to be used as novel diagnostic biomarkers for ACS. Twenty seven consecutive patients, admitted to emergency department of a training and research hospital between January-December 2013 with acute chest pain and/or dyspnea and diagnosed with ACS, and 16 non-ACS control subjects were included in this study. miRNA profiling was performed by using real time polymerase chain reaction. Functions of dysregulated miRNAs were evaluated by computerized-pathways analysis. miR-221-3p was one of the two most dysregulated miRNAs with a fold regulation of 3.89. It was significantly positively correlated with both Troponin and GRACE and Synthax Score. Moreover, miR221-3p was found to be significantly inversely correlated with left ventricular ejection fraction. miR-221-3p was the most prominent biomarker candidate with an area under curve (AUC) level of 0.881 (95% confidence interval: 0.774-0.987; p=0.002). The present study is the first to report an increased expression levels of miR-221-3p in AMI. Since miR-221-3p has a high discriminative value and significant relations with Troponin, GRACE and Synthax score and left ventricular systolic function, it may be a potential biomarker for early prediction of AMI. PMID:27374153

  10. MicroRNA-146b-3p Regulates Retinal Inflammation by Suppressing Adenosine Deaminase-2 in Diabetes

    Sadanand Fulzele

    2015-01-01

    Full Text Available Hyperglycemia- (HG- Amadori-glycated albumin- (AGA- induced activation of microglia and monocytes and their adherence to retinal vascular endothelial cells contribute to retinal inflammation leading to diabetic retinopathy (DR. There is a great need for early detection of DR before demonstrable tissue damages become irreversible. Extracellular adenosine, required for endogenous anti-inflammation, is regulated by the interplay of equilibrative nucleoside transporter with adenosine deaminase (ADA and adenosine kinase. ADA, including ADA1 and ADA2, exists in all organisms. However, because ADA2 gene has not been identified in mouse genome, how diabetes alters adenosine-dependent anti-inflammation remains unclear. Studies of pig retinal microglia and human macrophages revealed a causal role of ADA2 in inflammation. Database search suggested miR-146b-3p recognition sites in the 3′-UTR of ADA2 mRNA. Coexpression of miR-146b-3p, but not miR-146-5p or nontargeting miRNA, with 3′-UTR of the ADA2 gene was necessary to suppress a linked reporter gene. In the vitreous of diabetic patients, decreased miR-146b-3p is associated with increased ADA2 activity. Ectopic expression of miR-146b-3p suppressed ADA2 expression, activity, and TNF-α release in the AGA-treated human macrophages. These results suggest a regulatory role of miR-146b-3p in diabetes related retinal inflammation by suppressing ADA2.

  11. The deexcitation of the Ar (3P2, 3p1 and 1P1) states as measured by absorption both in pure argon and in the presence of additives

    The de-excitation of the 3P2, 3p1 and 1P1 states of argon was studied in pure argon between 10 and 200 torr and in Ar + CO and Ar + H2 mixtures. These states are populated after excitation of the gas by a short (20 ns) pulse of 500 keV electrons (FEBETRON). Under our experimental conditions, the relation between the measured optical density of the lines studied and the concentration of absorbing species was found to be: DO = log I0/I ∝ (lC)n with n = 0,4. The three body rate constants k2 were measured for the two resonant states 3p1 (k2 = (1,65 ± 0,3) x 10-32 cm6 s-1) and 1P1 (k2 = (1,0 ± 0,2) x 10-32 cm6 s-1); they had not been considered in previous low pressure studies. For the metastable state 3P2, the measured value of k2 ((1,6 ± 0,3) x 10-32 cm6 s-1) is in good agreement with those found in the literature. However, our two body rate constant k1 is about ten times higher than that found in measurements at low pressure. This difference could be due to a collision-induced emission process at high pressure. The rate constants for the quenching by CO and H2 were measured for the metastable state 3P2 (1,85 and 10,5 x 10-11 cm3 s-1) and for the resonant states 3P1 (4,5 and 20 x 10-11 cm3 s-1) and 1P1 (8,5 and 33 X 10-11 cm3 s-1). Comparison of the de-excitation cross sections of resonant and metastable states should lead to a better understanding of energy transfer processes from these latter. (author)

  12. Communication: Direct measurements of nascent O(3P0,1,2) fine-structure distributions and branching ratios of correlated spin-orbit resolved product channels CO(ã3Π; v) + O(3P0,1,2) and CO(Χ~1Σ+; v) + O(3P0,1,2) in VUV photodissociation of CO2

    We present a generally applicable experimental method for the direct measurement of nascent spin-orbit state distributions of atomic photofragments based on the detection of vacuum ultraviolet (VUV)-excited autoionizing-Rydberg (VUV-EAR) states. The incorporation of this VUV-EAR method in the application of the newly established VUV-VUV laser velocity-map-imaging-photoion (VMI-PI) apparatus has made possible the branching ratio measurement for correlated spin-orbit state resolved product channels, CO(ã3Π; v) + O(3P0,1,2) and CO(Χ~1Σ+; v) + O(3P0,1,2), formed by VUV photoexcitation of CO2 to the 4s(101) Rydberg state at 97,955.7 cm−1. The total kinetic energy release (TKER) spectra obtained from the O+ VMI-PI images of O(3P0,1,2) reveal the formation of correlated CO(ã3Π; v = 0–2) with well-resolved v = 0–2 vibrational bands. This observation shows that the dissociation of CO2 to form the spin-allowed CO(ã3Π; v = 0–2) + O(3P0,1,2) channel has no potential energy barrier. The TKER spectra for the spin-forbidden CO(Χ~1Σ+; v) + O(3P0,1,2) channel were found to exhibit broad profiles, indicative of the formation of a broad range of rovibrational states of CO(Χ~1Σ+)  with significant vibrational populations for v = 18–26. While the VMI-PI images for the CO(ã3Π; v = 0–2) + O(3P0,1,2) channel are anisotropic, indicating that the predissociation of CO2 4s(101) occurs via a near linear configuration in a time scale shorter than the rotational period, the angular distributions for the CO(Χ~1Σ+; v) + O(3P0,1,2) channel are close to isotropic, revealing a slower predissociation process, which possibly occurs on a triplet surface via an intersystem crossing mechanism

  13. Oscillator strength of the strongly 'forbidden' Pb 6p{sup 2} {sup 3}P{sub 0}{yields}6p{sup 2} {sup 3}P{sub 1} transition at 1278.9 nm

    Vadla, C.; Horvatic, V. [Inst. of Physics, Zagreb (Croatia); Niemax, K. [Inst. of Spectrochemistry and Applied Spectroscopy (ISAS), Dortmund (Germany)

    2001-04-01

    The 'forbidden' Pb 6p{sup 2} {sup 3}P{sub 0}{yields}6p{sup 2} {sup 3}P{sub 1} line at 1278.9 nm is measured by diode laser absorption in a resistively heated hot-pipe filled with a lead vapour and argon as buffer gas. The measurements performed at a temperature of 1170 K and a lead number density of 2.4 x 10{sup 15} cm{sup -3} yield the oscillator strength f{sub F}=(4.5{+-}1.1) x 10{sup -7} which corresponds to a radiative transition probability A{sub F}=(6.1{+-}1.5) s{sup -1}. Within the error bars, the result is in agreement with theoretical data published by different authors. (orig.)

  14. Compositional disorder and its effect on the thermoelectric performance of Zn3P2 nanowire–copper nanoparticle composites

    Recent studies indicated that nanowire format of materials is ideal for enhancing the thermoelectric performance of materials. Most of these studies were performed using individual nanowires as the test elements. It is not currently clear whether bulk assemblies of nanowires replicate this enhanced thermoelectric performance of individual nanowires. Therefore, it is imperative to understand whether enhanced thermoelectric performance exhibited by individual nanowires can be extended to bulk assemblies of nanowires. It is also imperative to know whether the addition of metal nanoparticle to semiconductor nanowires can be employed for enhancing their thermoelectric performance further. Specifically, it is important to understand the effect of microstructure and composition on the thermoelectric performance on bulk compound semiconductor nanowire–metal nanoparticle composites. In this study, bulk composites composed of mixtures of copper nanoparticles with either unfunctionalized or 1,4-benzenedithiol (BDT) functionalized Zn3P2 nanowires were fabricated and analyzed for their thermoelectric performance. The results indicated that use of BDT functionalized nanowires for the fabrication of composites leads to interface-engineered composites that have uniform composition all across their cross-section. The interface engineering allows for increasing their Seebeck coefficients and electrical conductivities, relative to the Zn3P2 nanowire pellets. In contrast, the use of unfunctionalized Zn3P2 nanowires for the fabrication of composite leads to the formation of composites that are non-uniform in composition across their cross-section. Ultimately, the composites were found to have Zn3P2 nanowires interspersed with metal alloy nanoparticles. Such non-uniform composites exhibited very high electrical conductivities, but slightly lower Seebeck coefficients, relative to Zn3P2 nanowire pellets. These composites were found to show a very high zT of 0.23 at 770 K, orders of

  15. Photoassociation spectroscopy of long-range molecular states below the 2s + 3p 6Li2 asymptote

    Gross, Christian; Chaudhuri, Saptarishi; Gan, Jaren; Dieckmann, Kai

    2015-05-01

    We present photoassociation spectra of high-lying vibrational states of the interatomic potentials correlating to the 2s + 3p asymptote of 6Li2 . Starting from an atomic cloud in a magneto-optical trap we first drive a free-to-bound transition into a molecular bound state using a tunable ultra-violet laser. Thereafter we ionize these long-range molecules using a 532 nm laser and detect the resulting ions with a channeltron. We determine the absolute positions of the transitions with MHz precision utilizing a frequency comb based calibration. Lithium dimers are extensively studied theoretically using various models and methods. Spectroscopic measurements are crucial to test and benchmark these methods and are available for various electronic states and inter-nuclear distances of 6Li2 molecule. Our study provides the first experimental observation of long-range states of the 2s + 3p asymptote of 6Li2 .

  16. Efficient transfer hydrogenation reaction Catalyzed by a dearomatized PN 3P ruthenium pincer complex under base-free Conditions

    He, Lipeng

    2012-03-01

    A dearomatized complex [RuH(PN 3P)(CO)] (PN 3PN, N′-bis(di-tert-butylphosphino)-2,6-diaminopyridine) (3) was prepared by reaction of the aromatic complex [RuH(Cl)(PN 3P)(CO)] (2) with t-BuOK in THF. Further treatment of 3 with formic acid led to the formation of a rearomatized complex (4). These new complexes were fully characterized and the molecular structure of complex 4 was further confirmed by X-ray crystallography. In complex 4, a distorted square-pyramidal geometry around the ruthenium center was observed, with the CO ligand trans to the pyridinic nitrogen atom and the hydride located in the apical position. The dearomatized complex 3 displays efficient catalytic activity for hydrogen transfer of ketones in isopropanol. © 2011 Elsevier B.V. All rights reserved.

  17. Novel Mad2-targeting miR-493-3p controls mitotic fidelity and cancer cells’ sensitivity to paclitaxel

    Tambe, Mahesh; Pruikkonen, Sofia; Mäki-Jouppila, Jenni; Ping CHEN; Elgaaen, Bente Vilming; Straume, Anne Hege; Huhtinen, Kaisa; Cárpen, Olli; Lønning, Per Eystein; Davidson, Ben; Hautaniemi, Sampsa; Kallio, Marko J.

    2016-01-01

    The molecular pathways that contribute to the proliferation and drug response of cancer cells are highly complex and currently insufficiently characterized. We have identified a previously unknown microRNA-based mechanism that provides cancer cells means to stimulate tumorigenesis via increased genomic instability and, at the same time, evade the action of clinically utilized microtubule drugs. We demonstrate miR-493-3p to be a novel negative regulator of mitotic arrest deficient-2 (MAD2), an...

  18. Wakefield Simulation of CLIC PETS Structure Using Parallel 3D Finite Element Time-Domain Solver T3P

    Candel, A.; Kabel, A.; Lee, L.; Li, Z.; Ng, C.; Schussman, G.; Ko, K.; /SLAC; Syratchev, I.; /CERN

    2009-06-19

    In recent years, SLAC's Advanced Computations Department (ACD) has developed the parallel 3D Finite Element electromagnetic time-domain code T3P. Higher-order Finite Element methods on conformal unstructured meshes and massively parallel processing allow unprecedented simulation accuracy for wakefield computations and simulations of transient effects in realistic accelerator structures. Applications include simulation of wakefield damping in the Compact Linear Collider (CLIC) power extraction and transfer structure (PETS).

  19. Candida albicans Als3p is required for wild-type biofilm formation on silicone elastomer surfaces

    Zhao, Xiaomin; Daniels, Karla J.; Oh, Soon-Hwan; GREEN, CLAYTON B.; Yeater, Kathleen M; Soll, David R.; Hoyer, Lois L.

    2006-01-01

    Candida albicans ALS3 encodes a large cell-surface glycoprotein that has adhesive properties. Immunostaining of cultured C. albicans germ tubes showed that Als3p is distributed diffusely across the germ tube surface. Two-photon laser scanning microscopy of model catheter biofilms grown using a PALS3-green fluorescent protein (GFP) reporter strain showed GFP production in hyphae throughout the biofilm structure while biofilms grown using a PTPI1-GFP reporter strain showed GFP in both hyphae an...

  20. The fast C(3P) + CH3OH reaction as an efficient loss process for gas-phase interstellar methanol

    Shannon, Robin J; Loison, Jean-Christophe; Caubet, Philippe; Balucani, Nadia; Seakins, Paul W; Wakelam, Valentine; Hickson, Kevin M

    2014-01-01

    Rate constants for the C(3P) + CH3OH reaction have been measured in a continuous supersonic flow reactor over the range 50 K to 296 K. C(3P) was created by the in-situ pulsed laser photolysis of CBr4, a multiphoton process which also produced some C(1D), allowing us to investigate simultaneously the low temperature kinetics of the C(1D) + CH3OH reaction. C(1D) atoms were followed by an indirect chemiluminescent tracer method in the presence of excess CH3OH. C(3P) atoms were detected by the same chemiluminescence technique and also by direct vacuum ultra-violet laser induced fluorescence (VUV LIF). Secondary measurements of product H(2S) atom formation have been undertaken allowing absolute H atom yields to be obtained by comparison with a suitable reference reaction. In parallel, statistical calculations have been performed based on ab-initio calculations of the complexes, adducts and transition states (TSs) relevant to the title reaction. By comparison with the experimental H atom yields, the preferred react...

  1. miR-130b-3p Upregulation Contributes to the Development of Thyroid Adenomas Targeting CCDC6 Gene

    Leone, Vincenza; Langella, Concetta; Esposito, Francesco; De Martino, Marco; Decaussin-Petrucci, Myriam; Chiappetta, Gennaro; Bianco, Antonio; Fusco, Alfredo

    2015-01-01

    We have previously studied the function of microRNAs (miRNAs) in thyroid cells using the differentiated rat thyroid PC Cl 3 cells that need thyrotropin (TSH) for their growth. The miRNA expression profile examination allowed the detection of a set of miRNAs downregulated and upregulated by TSH. Here, we first demonstrated that upregulation of miR-130b-3p occurs through a protein kinase A-cAMP-responsive element binding protein (CREB)-dependent mechanism. Then, we analyzed its expression in human thyroid follicular adenomas, where a constitutive CREB activation is frequently present. miR-130b-3p results in upregulation with a high fold-change in most thyroid follicular adenomas. Then, we identified CCDC6, coding for a protein that interacts with CREB1 leading to the transcriptional repression of CREB1 target genes, as a target of this miRNA. The targeting of CCDC6 by miR-130b-3p likely accounts for the mechanism by which its upregulation contributes to the development of thyroid adenomas increasing CREB1 activity. PMID:26835423

  2. Theoretical Studies on the Reaction Mechanisms of C3H2 (cyclopropenylidene) and O(3P) Radicals

    XlE Xiaohua; SHEN Wei; HE Rongxing; ZHANG Jinsheng; LI Ming

    2009-01-01

    The complex potential energy surface for the reaction of C3H2 (cyclopropenylidene) with O(3p) was explored computationally using a density functional and ab initio QCISD(T) methods. The geometries of all the stationary points (transition states, intermediates and products) were fully optimized at the B3LYP/6-311++G** computa-tional level, and the single point calculation including full population analysis was performed by employing QCISD(T). Our results show that the product P1 (C2H+HCO) is the major product, while the products P2 (C2H2+ CO) and P3 (HC3O+H) are minor products, as confirmed by experiment. Product P1 could be gained through the path: R→IM1→IM2→P1, and the C3H2+O(3P) reaction was expected to be rapid. So, the C3H2+O(3p) reaction may be an efficient strategy for producing C2H using cyclopropenylidene in atmosphere. The present results can lead us to understand deeply the mechanism of the title reaction.

  3. Inhibition of bacterial conjugation by phage M13 and its protein g3p: quantitative analysis and model.

    Abraham Lin

    Full Text Available Conjugation is the main mode of horizontal gene transfer that spreads antibiotic resistance among bacteria. Strategies for inhibiting conjugation may be useful for preserving the effectiveness of antibiotics and preventing the emergence of bacterial strains with multiple resistances. Filamentous bacteriophages were first observed to inhibit conjugation several decades ago. Here we investigate the mechanism of inhibition and find that the primary effect on conjugation is occlusion of the conjugative pilus by phage particles. This interaction is mediated primarily by phage coat protein g3p, and exogenous addition of the soluble fragment of g3p inhibited conjugation at low nanomolar concentrations. Our data are quantitatively consistent with a simple model in which association between the pili and phage particles or g3p prevents transmission of an F plasmid encoding tetracycline resistance. We also observe a decrease in the donor ability of infected cells, which is quantitatively consistent with a reduction in pili elaboration. Since many antibiotic-resistance factors confer susceptibility to phage infection through expression of conjugative pili (the receptor for filamentous phage, these results suggest that phage may be a source of soluble proteins that slow the spread of antibiotic resistance genes.

  4. BOT3P: a mesh generation software package for the transport analysis codes Dort, Tort, Twodant, Threedant and MCNP

    Bot3p consists of a set of standard Fortran 77 language programs that gives the users of the deterministic transport codes Dort and Tort some useful diagnostic tools to prepare and check the geometry of their input data files for both Cartesian and cylindrical geometries including graphical display modules. Bot3p produces at the same time the geometrical and material distribution data for the deterministic transport codes Twodant and Threedant and, only in three-dimensional (3D) Cartesian geometry, for the Monte Carlo Transport Code MCNP. This makes it possible to compare directly for the same geometry the effects stemming from the use of different data libraries and solution approaches on transport analysis results. Through the use of Bot3p, radiation transport problems with complex 3D geometrical structures can be modelled easily, as a relatively small amount of engineer-time is required and refinement is achieved by changing few parameters. This tool is useful for solving very large challenging problems. (author)

  5. Strong decays of χ{sub cJ}(2P) and χ{sub cJ}(3P)

    Wang, Hui; Ping, Jialun [Nanjing Normal University and Jiangsu Key Laboratory for Numerical Simulation of Large Scale Complex Systems, Department of Physics, Nanjing (China); Yang, Youchang [Zunyi Normal College, Department of Physics, Zunyi (China)

    2014-04-15

    In the framework of the chiral quark model, the mass spectrum of χ{sub cJ}(J = 0, 1, 2, n = 1, 2, 3) is studied with the Gaussian expansion method. Using the wave functions obtained in the study of mass spectrum, the open charm two-body strong decay widths of these states are calculated by using the {sup 3}P{sub 0} model. The results show that the masses of χ{sub cJ}(1P) and χ{sub c2}(2P) are consistent with the experimental data. But the strong decay width of χ{sub c2}(2P) is three times that of the experimental value. The decay width of χ{sub c1}(2P) is sensitive to its mass. In the quark-antiquark picture, the width is about 385 MeV. However, if the channel coupling effects shift its mass to 3872 MeV, its decay width will be around 1 MeV. The possibility of assigning the state X(3872) as χ{sub c1}(2P) cannot be excluded. To assign X(3915) as χ{sub c0}(2P) is disfavored, due to the unmatching of decay channel. For the χ{sub cJ}(3P) states, no states have been assigned. The possible candidates of χ{sub c0}(3P) are X(4160) and X(4140). Their masses are close to the theoretical ones. The experimental branching ratio of X(4160), Γ (X(4160) → D anti D)/Γ (X(4160) → D* anti D*) < 0.09 is compatible with that of χ{sub c0}(3P), 0.07. However the broad decay width of X(4160) cannot be explained by the open charm two-body decay. To assign X(4140) as χ{sub c0}(3P) is also possible, due to the compatibility of the total decay width, the further measurement of decay modes of X(4140) are expected to justify the assignment. (orig.)

  6. Electron Excitation Cross Sections for the S II Transitions: 3s(exp 2)3p(exp 3) 4S(exp o) approaches 3s(exp 2)3p(exp 3) 2D(exp o), 2P(exp o), and 3s3p(exp 4) 4P

    Liao, C.; Chutjian, A.; Hitz, D.; Tayal, S. S.

    1997-01-01

    Experimental and theoretical collisional excitation cross sections are reported for the transitions 3s(exp 2)3p(exp 3)4S(exp o) approaches 3s(exp 2)3p(exp 3) 2D(exp o), 2P(exp o), and 3s3P(exp 4) 4P in S II. The transition wavelengths (energies) are 6716 A (1.85 eV), 4069 A (3.05 eV), and 1256 A (9.87 eV), respectively. In the experiments, use is made of the energy-loss merged-beams method. The metastable fraction of the S II beam was assessed and minimized. The contribution of elastically scattered electrons was reduced by the use of a lowered solenoidal magnetic field and a modulated radio-frequency voltage on the analyzing plates and by retarding grids to reject the elastically scattered electrons with larger Larmor radii. For each transition, comparisons are made among experiments, the new 19 state R-matrix calculation, and three other close-coupling calculations.

  7. Liver MicroRNA-291b-3p Promotes Hepatic Lipogenesis through Negative Regulation of Adenosine 5'-Monophosphate (AMP)-activated Protein Kinase α1.

    Meng, Xiangyu; Guo, Jun; Fang, Weiwei; Dou, Lin; Li, Meng; Huang, Xiuqing; Zhou, Shutong; Man, Yong; Tang, Weiqing; Yu, Liqing; Li, Jian

    2016-05-13

    In a microarray study, we found that hepatic miR-291b-3p was significantly increased in leptin-receptor-deficient type 2 mice (db/db), a mouse model of diabetes. The function of miR-291b-3p is unknown. The potential role of miR-291b-3p in regulating hepatic lipid metabolism was explored in this study. High-fat diet (HFD)- and chow-fed mice were injected with an adenovirus expressing a miR-291b-3p inhibitor and a miR-291b-3p mimic through the tail vein. Hepatic lipids and lipogenic gene expression were analyzed. Additionally, gain- and loss-of-function studies were performed in vitro to identify direct targets of miR-291b-3p. MiR-291b-3p expression and the protein levels of sterol regulatory element-binding protein 1 (SREBP1) and fatty acid synthase (FAS) were increased in the steatotic liver of db/db mice and HFD-fed mice versus their respective controls. Inhibition of hepatic miR-291b-3p expression prevented increases in hepatic lipogenesis and steatosis in HFD-fed mice. The opposite was observed when miR-291b-3p was overexpressed in the livers of chow-fed C57BL/6J wild-type mice. In vitro studies revealed that silencing of miR-291b-3p in NCTC1469 hepatic cells ameliorated oleic acid/palmitic acid mixture-induced elevation of cellular triglycerides. Importantly, we identified AMP-activated protein kinase (AMPK)-α1 as a direct target of miR-291b-3p. Using metformin, an activator of AMPK, we showed that AMPK activation-induced inhibition of hepatic lipid accumulation was accompanied by reduced expression of miR-291b-3p in the liver. Liver miR-291b-3p promoted hepatic lipogenesis and lipid accumulation in mice. AMPKα1 is a direct target of miR-291b-3p. In conclusion, our findings indicate that miR-291b-3p promotes hepatic lipogenesis by suppressing AMPKα1 expression and activity, indicating the therapeutic potential of miR-291b-3p inhibitors in fatty liver disease. PMID:27013659

  8. WAVELENGTHS OF THE 3p-3d TRANSITIONS OF THE Co- AND Fe-LIKE IONS : THE EFFECTS OF ELECTRON CORRELATION

    Chen, M.

    1988-01-01

    The experimental observations of the 3p6 3d9 2D - 3p5 3dl0 2p transitions of the Co-like ions and 3p6 3d8 3F4 - 3p5 3d9 3F3 of the Fe-like ions have recently been extended to highly charged ions of heavy elements up to uranium (Z = 92). A comparison between the observed energies and calculated values from the Dirac-Fock model indicated persistent diicrepancies of 3-4 eV for al1 ions. Systematic multiconfiguration Dirac-Fock caiculations for these transitions have been carried out with emphasi...

  9. Additional investigations of silicon phosphides (SiP and Si3P2) at phosphor-diffused silicon wafers

    Results were obtained by X-ray microanalysis and neutron activation analysis. The formation of the Si3P2-phase (circular particles of about 15 μm in diameter) takes place on polished silicon slices during the phosphorus diffusion both in N2-O2-POCl3 carrier gas and in O2-P2O5 carrier gas. In this case the average phosphorus concentration in phosphorus glass sheet (reaction phase, i.e. SiO2-P2O5-phase) is greater than the limit value being placed in the interval c1 = (8,30 ... 9,44) mass-percent phosphorus for a temperature T = 1523 K (12500C). The surface concentration of phosphorus in silicon between the Si3P2-particles being formed on slices does not depend on the phosphorus concentration in the carrier gas. This constant concentration value is identical with the solid solubility limit for phosphorus in silicon, measured for C/sub F/ = 1.29 x 1021 P-atoms/cm3, resp. c/sub F/ = 2.72 mass percent. On the other hand the phosphorus concentration is C/sub G/ = 9.2 x 1020 P-atoms/cm3 in the interior of the diffused layer in silicon directly beside the Si3P2-particle. The formation of the SiP-phase was observed on lapped silicon slices for phosphorus diffusion in wet N2-O2-POCl3 carrier gas at a temperature T = 1523 K (12500C). (author)

  10. The microRNA miR-17-3p inhibits mouse cardiac fibroblast senescence by targeting Par4.

    Du, William W; Li, Xianmin; Li, Tianbi; Li, Haoran; Khorshidi, Azam; Liu, Fengqiong; Yang, Burton B

    2015-01-15

    The microRNA miR-17-92 cluster plays a fundamental role in heart development. The aim of this study was to investigate the effect of a member of this cluster, miR-17, on cardiac senescence. We examined the roles of miR-17 in senescence and demonstrated that miR-17-3p attenuates cardiac aging in the myocardium by targeting Par4 (also known as PAWR). This upregulates the downstream proteins CEBPB, FAK, N-cadherin, vimentin, Oct4 and Sca-1 (also known as stem cell antigen-1), and downregulates E-cadherin. Par4 has been reported as a tumor suppressor gene that induces apoptosis in cancer cells, but not in normal cells. Repression of Par4 by miR-17-3p enhances the transcription of CEBPB and FAK, which promotes mouse cardiac fibroblast (MCF) epithelial-to-mesenchymal transition (EMT) and self-renewal, resulting in cellular senescence and apoptosis resistance. We conclude that Par4 can bind to the CEBPB promoter and inhibit its transcription. Decreased Par4 expression increases the amount of CEBPB, which binds to the FAK promoter and enhances FAK transcription. Par4, CEBPB and FAK form a senescence signaling pathway, playing roles in modulating cell survival, growth, apoptosis, EMT and self-renewal. Through this novel senescence signaling axis, miR-17-3p represses Par4 expression, acting pleiotropically as a negative modulator of cardiac aging and cardiac fibroblast cellular senescence. PMID:25472717

  11. Photovoltaic effect of n-In_2O_3/p-Cu_2O heterojunction thin film cells

    NAKAZAWA, Tatsuo; KURANOUCHI, Shin'ichi

    1992-01-01

    Electrical and photovoltaic characteristics of n-In_2O_3/p-Cu_2O heterojunction thin film cells were investigated. Cu_2O semiconducting films were prepared by simple method of thermal oxidation of copper foil in air. Conversion efficiency of 0.57% was obtained under illumination of the sunlight. It is thought that a high series resistance of the diode, which originated from a interface between Cu_2O film and copper foil, causes the poor characteristics. Photovoltaic characteristics of the cel...

  12. Human mitochondrial RNA turnover caught in flagranti: involvement of hSuv3p helicase in RNA surveillance

    Szczesny, Roman J; Borowski, Lukasz S.; Brzezniak, Lien K.; Dmochowska, Aleksandra; Gewartowski, Kamil; Bartnik, Ewa; Stepien, Piotr P.

    2009-01-01

    The mechanism of human mitochondrial RNA turnover and surveillance is still a matter of debate. We have obtained a cellular model for studying the role of hSuv3p helicase in human mitochondria. Expression of a dominant-negative mutant of the hSUV3 gene which encodes a protein with no ATPase or helicase activity results in perturbations of mtRNA metabolism and enables to study the processing and degradation intermediates which otherwise are difficult to detect because of their short half-lives...

  13. Two-photon ionisation of metastable helium: complex structure in the 2S-3P resonance profiles

    The wavelength dependence of resonant two-photon ionisation of metastable helium via the 3P intermediate level is reported. In both the singlet and triplet systems an additional complex, but reproducible, structure is seen in the resonance profile of the ion signal. This occurs for moderate laser intensities strong enough to saturate the resonant transition. The physical reason for the structure is not understood, but measurements point to a delicate dependence on the time development of the atomic states and the laser pulse. (author)

  14. Fum3p, a 2-Ketoglutarate-Dependent Dioxygenase Required for C-5 Hydroxylation of Fumonisins in Fusarium verticillioides

    Ding, Yousong; Bojja, Ravi S.; Du, Liangcheng

    2004-01-01

    Fumonisins are polyketide-derived mycotoxins produced by several agriculturally important Fusarium species. The B series fumonisins, FB1, FB2, FB3, and FB4, are fumonisins produced by wild-type Fusarium verticillioides strains, differing in the number and location of hydroxyl groups attached to the carbon backbone. We characterized the protein encoded by FUM3, a gene in the fumonisin biosynthetic gene cluster. The 33-kDa FUM3 protein (Fum3p) was heterologously expressed and purified from Sacc...

  15. Remote frequency measurement of the 1S0-3P1 transition in laser cooled Mg-24

    Friebe, J.; Riedmann, M.; Wübbena, T.; Pape, A.; Kelkar, H.; Ertmer, W.; Terra, O.; Sterr, U.; Weyers, S.; Grosche, G.; Schnatz, H.; Rasel, E. M.

    2011-01-01

    We perform Ramsey-Bord\\'e spectroscopy on laser-cooled magnesium atoms in free fall to measure the 1S0 \\rightarrow 3P1 intercombination transition frequency. The measured value of 655 659 923 839 730 (48) Hz is consistent with our former atomic beam measurement (Friebe et al 2008 Phys. Rev. A 78 033830). We improve upon the fractional accuracy of the previous measurement by more than an order of magnitude to 7e-14. The magnesium frequency standard was referenced to a fountain clock of the Phy...

  16. Formation of deeply bound ultracold Sr_2 molecules by photoassociation near the ^1S + ^3P_1 intercombination line

    Skomorowski, Wojciech; Moszynski, Robert; Koch, Christiane P.

    2012-01-01

    We predict feasibility of the photoassociative formation of Sr_2 molecules in arbitrary vibrational levels of the electronic ground state based on state-of-the-art ab initio calculations. Key is the strong spin-orbit interaction between the c^3\\Pi_u, A^1\\Sigma_u^+ and B^1\\Sigma_u^+ states. It creates not only an effective dipole moment allowing free-to-bound transitions near the ^1S + ^3P_1 intercombination line but also facilitates bound-to-bound transitions via resonantly coupled excited st...

  17. Cu3P

    Villars, P.; Cenzual, K.; Daams, J.; Gladyshevskii, R.; Shcherban, O.; Dubenskyy, V.; Melnichenko-Koblyuk, N.; Pavlyuk, O.; Savysyuk, I.; Stoyko, S.; Sysa, L.

    This document is part of Subvolume A6 `Structure Types. Part 6: Space Groups (166) R-3m - (160) R3m' of Volume 43 `Crystal Structures of Inorganic Compounds' of Landolt-Börnstein - Group III `Condensed Matter'.

  18. Effects on intercombination transition rates and branching ratios-the UV0.01 (3s{sup 2}3p{sup 2} {sup 3}P{sub 1,2} - 3s3p{sup 3} {sup 5}S{sub 2}) multiplet in Si I-like ions revisited

    Andersson, Martin; Brage, Tomas [Department of Physics, Lund University, Box 118, SE-221 00 Lund (Sweden)

    2007-02-28

    We report on large-scale ab initio calculations for intercombination lines in Si I-like ions. Two measurable quantities, the lifetime of the 3s3p{sup 3} {sup 5}S{sub 2} level and the branching ratio of the two lines are discussed, to infer the importance of different features of our calculations. The effects of core polarization, two-body spin-dependent operators and Dirac contra Breit-Pauli approaches are discussed. Earlier calculations are reviewed and evaluated. The calculated lifetimes are in good agreement with experiments, while a discrepancy persists for branching ratios.

  19. Puf3p, a Pumilio family RNA binding protein, localizes to mitochondria and regulates mitochondrial biogenesis and motility in budding yeast

    García-Rodríguez, Luis J.; Gay, Anna Card; Pon, Liza A.

    2007-01-01

    Puf3p binds preferentially to messenger RNAs (mRNAs) for nuclear-encoded mitochondrial proteins. We find that Puf3p localizes to the cytosolic face of the mitochondrial outer membrane. Overexpression of PUF3 results in reduced mitochondrial respiratory activity and reduced levels of Pet123p, a protein encoded by a Puf3p-binding mRNA. Puf3p levels are reduced during the diauxic shift and growth on a nonfermentable carbon source, conditions that stimulate mitochondrial biogenesis. These findings support a role for Puf3p in mitochondrial biogenesis through effects on mRNA interactions. In addition, Puf3p links the mitochore, a complex required for mitochondrial–cytoskeletal interactions, to the Arp2/3 complex, the force generator for actin-dependent, bud-directed mitochondrial movement. Puf3p, the mitochore, and the Arp2/3 complex coimmunoprecipitate and have two-hybrid interactions. Moreover, deletion of PUF3 results in reduced interaction between the mitochore and the Arp2/3 complex and defects in mitochondrial morphology and motility similar to those observed in Arp2/3 complex mutants. Thus, Puf3p is a mitochondrial protein that contributes to the biogenesis and motility of the organelle. PMID:17210948

  20. 2s 2p 3P10 → 2s21S0 intercombination line in beryllium-like krypton, molybdenum and tungsten

    Transition probabilities are evaluated for the 2s 2p 3P10 → 2s21S0 transition in beryllium-like ions for krypton, molybdenum and tungsten, using configuration-interaction wavefunctions. The importance of the 2s 3p 1P10 configuration is considered

  1. miR-625-3p regulates oxaliplatin resistance by targeting MAP2K6-p38 signalling in human colorectal adenocarcinoma cells

    Rasmussen, Mads Heilskov; Lyskjær, Iben; Jersie-Christensen, Rosa Rakownikow;

    2016-01-01

    Oxaliplatin resistance in colorectal cancers (CRC) is a major medical problem, and predictive markers are urgently needed. Recently, miR-625-3p was reported as a promising predictive marker. Herein, we show that miR-625-3p functionally induces oxaliplatin resistance in CRC cells, and identify the...

  2. Experimental Determination of the 24Mg I (3s3p)3P2 Lifetime

    Jensen, Brian Bak; He, Ming; Westergaard, Philip Grabow;

    2011-01-01

    We present the first experimental determination of the electric-dipole forbidden (3s3p)3P2¿(3s2)1S0 (M2) transition rate in 24Mg and compare to state-of-the-art theoretical predictions. Our measurement exploits a magnetic trap isolating the sample from perturbations and a magneto-optical trap as an...

  3. A study of angle-resolved photoemission extended fine structure as applied to the Ni 3p, Cu 3s, and Cu 3p core levels of the respective clean (111) surfaces

    Huff, W.R.A.; Moler, E.J.; Kellar, S.A. [Lawrence Berkeley National Lab., CA (United States)] [and others

    1997-04-01

    The first non-s initial state angle-resolved photoemission extended fine structure (ARPEFS) study of clean surfaces for the purpose of further understanding the technique is reported. The surface structure sensitivity of ARPEFS applied to clean surfaces and to arbitrary initial states is studied using normal photoemission data taken from the Ni 3p core levels of a Ni(111) single crystal and the Cu 3s and the Cu 3p core-levels of a Cu(111) single crystal. The Fourier transforms of these clean surface data are dominated by backscattering. Unlike the s initial state data, the p initial state data show a peak in the Fourier transform corresponding to in-plane scattering from the six nearest-neighbors to the emitter. Evidence was seen for single-scattering events from in the same plane as the emitters and double-scattering events. Using a newly developed, multiple-scattering calculation program, ARPEFS data from clean surfaces and from p initial states can be modeled to high precision. Although there are many layers of emitters when measuring photoemission from a clean surface, test calculations show that the ARPEFS signal is dominated by photoemission from atoms in the first two crystal layers. Thus, ARPEFS applied to clean surfaces is sensitive to surface reconstruction. The known contraction of the first two Cu(111) layers is confirmed. The best-fit calculation for clean Ni(111) indicates an expansion of the first two layers. To better understand the ARPEFS technique, the authors studied s and non-s initial state photoemission from clean metal surfaces.

  4. A study of angle-resolved photoemission extended fine structure as applied to the Ni 3p, Cu 3s, and Cu 3p core levels of the respective clean (111) surfaces

    The first non-s initial state angle-resolved photoemission extended fine structure (ARPEFS) study of clean surfaces for the purpose of further understanding the technique is reported. The surface structure sensitivity of ARPEFS applied to clean surfaces and to arbitrary initial states is studied using normal photoemission data taken from the Ni 3p core levels of a Ni(111) single crystal and the Cu 3s and the Cu 3p core-levels of a Cu(111) single crystal. The Fourier transforms of these clean surface data are dominated by backscattering. Unlike the s initial state data, the p initial state data show a peak in the Fourier transform corresponding to in-plane scattering from the six nearest-neighbors to the emitter. Evidence was seen for single-scattering events from in the same plane as the emitters and double-scattering events. Using a newly developed, multiple-scattering calculation program, ARPEFS data from clean surfaces and from p initial states can be modeled to high precision. Although there are many layers of emitters when measuring photoemission from a clean surface, test calculations show that the ARPEFS signal is dominated by photoemission from atoms in the first two crystal layers. Thus, ARPEFS applied to clean surfaces is sensitive to surface reconstruction. The known contraction of the first two Cu(111) layers is confirmed. The best-fit calculation for clean Ni(111) indicates an expansion of the first two layers. To better understand the ARPEFS technique, the authors studied s and non-s initial state photoemission from clean metal surfaces

  5. MiR-291b-3p Induces Apoptosis in Liver Cell Line NCTC1469 by Reducing the Level of RNA-binding Protein HuR

    Jun Guo

    2014-03-01

    Full Text Available Background: There is increasing evidence that miRNAs are involved in cellular apoptosis. However, the specific role of miR-291b-3p in apoptosis has not been elucidated. In the present study, we investigated the effect of miR-291b-3p on NCTC1469 cell growth and apoptosis. Methods: Cell viability and apoptosis were examined in NCTC1469 cells transfected with miR-291b-3p mimics, inhibitor miRNA or negative control. Using computational miRNA target prediction databases, HuR was predicted as a target of miR-291b-3p. Luciferase assay, immunofluorescence and western blot were used to further explore the effects of miR-291b-3p on HuR expression. In addition, the effect of HuR on cell apoptosis was evaluated using a HuR-specific siRNA. Results: TNF-α-induced hepatocyte apoptosis was accompanied by enhanced expression of miR-291b-3p, suggesting that miR-291b-3p might contribute to the apoptotic process. Follow-up experiments showed that upregulation of miR-291b-3p decreased cell viability and induced NCTC1469 cell apoptosis. Additionally, similar to the activity of miR-519, which is another member of the same miRNA family, miR-291b-3p suppressed HuR translation through binding to the HuR coding region (CR. We further showed that the downregulation of HuR expression by miR-291b-3p was accompanied by reduced Bcl-2 expression. Moreover, knockdown of HuR also impaired Bcl-2 expression and increased the ratio of Bax/Bcl-2. More significantly, downregulation of miR-291b-3p failed to increase Bcl2 expression in NCTC1469 cells that were co-transfected with siRNA-HuR. Finally, inhibition of miR-291b-3p led to reduced apoptosis, while knockdown of HuR by siRNA promoted apoptosis, even in NCTC1469 cells that were co-transfected with the miR-291b-3p inhibitor. Conclusion: The current data suggested that miR-291b-3p contributed to NCTC1469 cell apoptosis by regulating the expression of HuR, which in turn increased Bcl-2 stability.

  6. Application of an electro-optical modulator in inelastic collision studies with laser-excited Na*(3p) atoms

    In atomic collision experiments with laser-excited target atoms it is often desirable to maximize the fraction f* of excited atoms. In the case of Na atoms pumped by a single mode CW dye laser radiation several effects including hyperfine pumping limit f* to about 10%. Using two side-bands of laser radiation, produced by means of an electro-optical modulator (phase modulation of laser radiation by a LiTaO3 crystal in a microwave field) transitions from both hyperfine ground states of Na can be pumped simultaneously. In this way a significant increase of the fraction of excited Na atoms in the 32P states to typically 30% could be achieved. The advantage of using an electro-optical modulator is demonstrated for the case of state-selective single electron capture studies of the He2+-Na*(3p) collision system. (author)

  7. Synthesis and characterization of Fe3P composite nanorods produced by phosphate reduction in anodized alumina templates

    A synthetic method for nanoscale iron phosphide rod production based on reductive annealing of iron phosphate deposited in porous alumina membranes has been explored. Two methods of pore filling, incipient wetness and drip-drying of precursor phosphate particles, were investigated. Reduction was carried out in a flowing H2/Ar atmosphere at 650-800 deg. C for 2 h and the template was removed by dissolution in NaOH. Particle morphology, composition and phase were examined by scanning electron microscopy, transmission electron microscopy, scanning transmission electron microscopy and energy-dispersive spectroscopy. The data suggest that rods are formed as heterogeneous structures comprising Fe3P nanoparticles within an amorphous matrix

  8. Phase-formation in K2O-Er2O3-P2O5-H2O system

    The K2O-Er2O3-P2O5-H2O system in the condensed phosphates crystallization at 300 and 500 deg C and constant pressUre of saturated water vapours by the method is investigated. Crystallization tregions of eight condensed erbium phosphates haVe been found out: two varieties of ErP5O14 ultraphosphates, two Er(PO3)3 polyphospates (Q and R phases), KErP4O12-A double cyclotetraphosphate and three double KEr)/PO3)4 of 3, 4 and 7 types, polyphosphates the KEr(PO3)4 - 7 and Er(PO3)3 - R types are obtained for the first time. Roentgenometric, morphological, crystalloptical and IR spectroscopic characteristics of condensed erbium phosphates are given

  9. Interactions of solute (3p, 4p, 5p and 6p) with solute, vacancy and divacancy in bcc Fe

    You, Yu-Wei; Kong, Xiang-Shan; Wu, Xue-Bang; Liu, Wei [Key Laboratory of Materials Physics, Institute of Solid State Physics, Chinese Academy of Sciences, P.O. Box 1129, Hefei 230031 (China); Liu, C.S., E-mail: csliu@issp.ac.cn [Key Laboratory of Materials Physics, Institute of Solid State Physics, Chinese Academy of Sciences, P.O. Box 1129, Hefei 230031 (China); Fang, Q.F. [Key Laboratory of Materials Physics, Institute of Solid State Physics, Chinese Academy of Sciences, P.O. Box 1129, Hefei 230031 (China); Chen, J.L.; Luo, G.-N. [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031 (China); Wang, Zhiguang [Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000 (China)

    2014-12-15

    Solute–vacancy binding energy is a key quantity in understanding solute diffusion kinetics and phase segregation, and may help choice of alloy compositions for future material design. However, the binding energy of solute with vacancy is notoriously difficult to measure and largely unknown in bcc Fe. With first-principles method, we systemically calculate the binding energies of solute (3p, 4p, 5p and 6p alloying solutes are included) with vacancy, divacancy and solute in bcc Fe. The binding energy of Si with vacancy in the present work is in good consistent with experimental value available. All the solutes considered are able to form stable solute–vacancy, solute–divacancy complexes, and the binding strength of solute–divacancy is about two times larger than that of solute–vacancy. Most solutes could not form stable solute–solute complexes except S, Se, In and Tl. The factors controlling the binding energies are analyzed at last.

  10. On mechanism of Ar(3p54p) states excitation in low-energy Ar-Ar collisions

    The present work is devoted to study of Ar(3p54p) states excitation in binary low-energy Ar-Ar collisions. The results of the experimental investigation of excitation cross sections of Ar I 4p'[l/2]1, 4p'[3/2]1, 4p'[3/2]2 and 4p[3/2]2 levels in the collision energy range from threshold up to 500 eV (cm) and degree of polarization for 4s[3/2]20-4p'[l/2]1 and 4s[3/2]20-4p[3/2]2 transitions in this energy range are represented.

  11. Interactions of solute (3p, 4p, 5p and 6p) with solute, vacancy and divacancy in bcc Fe

    Solute–vacancy binding energy is a key quantity in understanding solute diffusion kinetics and phase segregation, and may help choice of alloy compositions for future material design. However, the binding energy of solute with vacancy is notoriously difficult to measure and largely unknown in bcc Fe. With first-principles method, we systemically calculate the binding energies of solute (3p, 4p, 5p and 6p alloying solutes are included) with vacancy, divacancy and solute in bcc Fe. The binding energy of Si with vacancy in the present work is in good consistent with experimental value available. All the solutes considered are able to form stable solute–vacancy, solute–divacancy complexes, and the binding strength of solute–divacancy is about two times larger than that of solute–vacancy. Most solutes could not form stable solute–solute complexes except S, Se, In and Tl. The factors controlling the binding energies are analyzed at last

  12. Magnetic control of ultra-cold $^6$Li and $^{174}$Yb($^3P_2$) atom mixtures with Feshbach resonances

    Petrov, Alexander; Kotochigova, Svetlana

    2015-01-01

    We theoretically evaluate the feasibility to form magnetically-tunable Feshbach molecules in collisions between fermionic $^6$Li atoms and bosonic metastable $^{174}$Yb($^3$P$_2$) atoms. In contrast to the well-studied alkali-metal atom collisions, collisions with meta-stable atoms are highly anisotropic. Our first-principle coupled-channel calculation of these collisions reveals the existence of broad Feshbach resonances due to the combined effect of anisotropic-molecular and atomic-hyperfine interactions. In order to fit our predictions to the specific positions of experimentally-observed broad resonance structures \\cite{Deep2015} we optimized the shape of the short-range potentials by direct least-square fitting. This allowed us to identify the dominant resonance by its leading angular momentum quantum numbers and describe the role of collisional anisotropy in the creation and broadening of this and other resonances.

  13. Theoretical investigation of the dynamics of O(1D→3P) electronic quenching by collision with Xe

    We present the quantum close-coupling treatment of spin-orbit induced transitions between the 1D and 3P states of an atom in collisions with a closed-shell spherical partner. In the particular case of O colliding with Xe, we used electronic structure calculations to compute the relevant potential energy curves and spin-orbit coupling matrix elements. We then carried out quantum scattering calculations of integral and differential quenching cross sections as functions of the collision energy. The calculated differential cross sections for electronic quenching are in reasonable agreement with measurements [Garofalo et al., J. Chem. Phys. 143, 054307 (2015)]. The differential cross sections exhibit pronounced oscillations as a function of the scattering angle. By a semiclassical analysis, we show that these oscillations result from quantum mechanical interference between two classical paths

  14. Electron-phonon superconductivity in APt3P (A=Sr, Ca, La) compounds: From weak to strong coupling

    Subedi, Alaska; Ortenzi, Luciano; Boeri, Lilia

    2013-04-01

    We study the newly discovered Pt phosphides APt3P (A=Sr, Ca, La) [T. Takayama , Phys. Rev. Lett.PRLTAO0031-900710.1103/PhysRevLett.108.237001 108, 237001 (2012)] using first-principles calculations and Migdal-Eliashberg theory. Given the remarkable agreement with the experiment, we exclude the charge-density wave scenario proposed by previous first-principles calculations, and give conclusive answers concerning the superconducting state in these materials. The pairing increases from La to Ca and Sr due to changes in the electron-phonon matrix elements and low-frequency phonons. Although we find that all three compounds are well described by conventional s-wave superconductivity and spin-orbit coupling of Pt plays a marginal role, we show that it could be possible to tune the structure from centrosymmetric to noncentrosymmetric opening new perspectives towards the understanding of unconventional superconductivity.

  15. Polarization effects in metastable neon atom (Ne* ( 3P 2)) on ground state neon atom collision at thermal energy

    Baudon, J.; Perales, F.; Miniatura, Ch.; Robert, J.; Vassilev, G.; Reinhardt, J.; Haberland, H.

    1990-08-01

    The difference Δ between the differential cross section for Ne*( 3P 2 atoms polarized either in state | j=2, m=+2 > or | j=2, m=-2 >, colliding at thermal energy with a groundstate target (Ne,O 2), is measured. In the symmetric case Ne*-Ne, direct and exchange contribution are observed. General properties of Δ, derived from symmetry considerations, are established; in particular: (i) the interference character of Δ, (ii) the role played by the azimuthal dependence of the scattering amplitudes, (iii) the property Δ(0) = Δ(180°)=0. The relationship between the Fourier harmonics introduced in this discussion and the scattering amplitudes used in standard collision treatments are given.

  16. The C(3P) + NH3 reaction in interstellar chemistry: I. Investigation of the product formation channels

    Bourgalais, Jeremy; Kailasanathan, Ranjith Kumar Abhinavam; Osborn, David L; Hickson, Kevin M; Loison, Jean-Christophe; Wakelam, Valentine; Goulay, Fabien; Picard, Sébastien D Le

    2016-01-01

    The product formation channels of ground state carbon atoms, C(3P), reacting with ammonia, NH3, have been investigated using two complementary experiments and electronic structure calculations. Reaction products are detected in a gas flow tube experiment (330 K, 4 Torr) using tunable VUV photoionization coupled with time of flight mass spectrometry. Temporal profiles of the species formed and photoionization spectra are used to identify primary products of the C + NH3 reaction. In addition, H-atom formation is monitored by VUV laser induced fluorescence from room temperature to 50 K in a supersonic gas flow generated by the Laval nozzle technique. Electronic structure calculations are performed to derive intermediates, transition states and complexes formed along the reaction coordinate. The combination of photoionization and laser induced fluorescence experiments supported by theoretical calculations indicate that in the temperature and pressure range investigated, the H + H2CN production channel represents ...

  17. miR-199a-3p targets CD44 and reduces proliferation of CD44 positive hepatocellular carcinoma cell lines

    Henry, Jon C. [Department of Surgery, Ohio State University Medical Center, Columbus, OH (United States); Park, Jong-Kook; Jiang, Jinmai; Kim, Ji Hye [College of Pharmacy, Ohio State University, Columbus, OH (United States); Nagorney, David M.; Roberts, Lewis R. [Divisions of Gastroenterology and Hepatology, Mayo Clinic College of Medicine, Rochester, MN (United States); Banerjee, Soma [Center for Liver Research, School of Digestive and Liver Diseases, Institute of Post Graduate Medical Education and Research, Kolkata (India); Schmittgen, Thomas D., E-mail: Schmittgen.2@osu.edu [College of Pharmacy, Ohio State University, Columbus, OH (United States)

    2010-12-03

    Research highlights: {yields} miR-199a-3p targets CD44 in HCC. {yields} Proliferation and invasion are reduced by miR-199a-3p in CD44+ HCC. {yields} miR-199a-3p is reduced and CD44 protein is increased in HCC tissues. {yields} The duplex form of miR-199a-3p mimetic is required for activity. -- Abstract: Previous work by us and others reported decreased expression of miR-199a-3p in hepatocellular carcinoma (HCC) tissues compared to adjacent benign tissue. We report here a significant reduction of miR-199a-3p expression in 7 HCC cell lines. To determine if miR-199a-3p has a tumor suppressive role, pre-miR-199a-3p oligonucleotides were transfected into the HCC cell lines. Pre-miR-199a-3p oligonucleotide reduced cell proliferation by approximately 60% compared to control oligonucleotide in only two cell lines (SNU449 and SNU423); the proliferation of the other 5 treated cell lines was similar to control oligonucleotide. A pre-miR-199a-3p oligonucleotide formulated with chemical modifications to enhance stability while preserving processing, reduced cell proliferation in SNU449 and SNU423 to the same extent as the commercially available pre-miR-199a-3p oligonucleotide. Furthermore, only the duplex miR-199a-3p oligonucleotide, and not the guide strand alone, was effective at reducing cell viability. Since a CD44 variant was essential for c-Met signaling [V. Orian-Rousseau, L. Chen, J.P. Sleeman, P. Herrlich, H. Ponta, CD44 is required for two consecutive steps in HGF/c-Met signaling, Genes Dev. 16 (2002) 3074-3086] and c-Met is a known miR-199a-3p target, we hypothesized that miR-199a-3p may also target CD44. Immunoblotting confirmed that only the two HCC lines that were sensitive to the effects of pre-miR-199a-3p were CD44+. Direct targeting of CD44 by miR-199a-3p was confirmed using luciferase reporter assays and immunoblotting. Transfection of miR-199a-3p into SNU449 cells reduced in vitro invasion and sensitized the cells to doxorubicin; both effects were enhanced

  18. Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substrates

    Emmanuel M Papamichael

    1999-01-01

    Full Text Available In this study, the S1 - P1 and S3 - P3 interactions between papain and four synthetic peptide substrates were found as to be important. The values of Km were estimated as to be practically identical between these substrates; this latter is supporting the conclusions obtained by considering the estimated values of other kinetic parameters. Nevertheless, based on the estimated kcat and/or k cat/Km parameters of the used substrates, we concluded that an aromatic ring at the P3 position, and a positively charged side chain of the residue at the P1 position of the synthetic substrates were favored considerably their interaction with papain.Neste estudo, o S1 - P1 e S3 - P3, interações entre papaina e quatro substratos sintéticos de pepetídios foram considerados importantes. Os valores de Km foram estimados e são praticamente idênticos entre esses substratos; Isso dá suporte as conclusões obtidas, considerando os valores parâmetros cinéticos estimados. No obstante, baseou na estimação parâmetros kcat e/ou k cat /Km dos substratos utilizados. Se pode concluir que um anel aromático na posição P3, e uma corrente carregada positivamente da cadeia do resíduo na posição P1 dos substratos sintéticos favoreceram interação com a papaina.

  19. Major weapon system environmental life-cycle cost estimating for Conservation, Cleanup, Compliance and Pollution Prevention (C3P2)

    Hammond, Wesley; Thurston, Marland; Hood, Christopher

    1995-01-01

    The Titan 4 Space Launch Vehicle Program is one of many major weapon system programs that have modified acquisition plans and operational procedures to meet new, stringent environmental rules and regulations. The Environmental Protection Agency (EPA) and the Department of Defense (DOD) mandate to reduce the use of ozone depleting chemicals (ODC's) is just one of the regulatory changes that has affected the program. In the last few years, public environmental awareness, coupled with stricter environmental regulations, has created the need for DOD to produce environmental life-cycle cost estimates (ELCCE) for every major weapon system acquisition program. The environmental impact of the weapon system must be assessed and budgeted, considering all costs, from cradle to grave. The Office of the Secretary of Defense (OSD) has proposed that organizations consider Conservation, Cleanup, Compliance and Pollution Prevention (C(sup 3)P(sup 2)) issues associated with each acquisition program to assess life-cycle impacts and costs. The Air Force selected the Titan 4 system as the pilot program for estimating life-cycle environmental costs. The estimating task required participants to develop an ELCCE methodology, collect data to test the methodology and produce a credible cost estimate within the DOD C(sup 3)P(sup 2) definition. The estimating methodology included using the Program Office weapon system description and work breakdown structure together with operational site and manufacturing plant visits to identify environmental cost drivers. The results of the Titan IV ELCCE process are discussed and expanded to demonstrate how they can be applied to satisfy any life-cycle environmental cost estimating requirement.

  20. MicroRNA-219-2-3p functions as a tumor suppressor in gastric cancer and is regulated by DNA methylation.

    Huizi Lei

    Full Text Available BACKGROUND AIMS: Gastric cancer is the most frequent gastrointestinal tumor in adults and is the most lethal form of human cancer. Despite of the improvements in treatments, the underlying mechanism of gastric carcinogenesis is not well known. To define novel modulators that regulate susceptibility to tumorgenesis, we focused on miR-219-2-3p. METHODS: Quantitative RT-PCR was employed to investigate the level of miR-219-2-3p in gastric cancer (GC tissues (n = 113 and their matched adjacent normal tissues (n = 113. In vitro cell proliferation, apoptosis assays, cell migration, and invasion assays were performed to elucidate biological effects of miR-219-2-3p. Since silencing of miRNA by promoter CpG island methylation may be an important mechanism in tumorgenesis, GC cells were treated with 5-aza-2'-deoxycytidine and trichostatin A, and expression changes of miR-219-2-3p were subsequently examined by quantitative RT-PCR. Finally, the methylation status of CpG island upstream of miR-219-2-3p was analyzed by methylation-specific PCR in GC tissues (n = 22. RESULTS: miR-219-2-3p was down-regulated in GC and cell lines. In addition, the experiments documented the lower expression of miR-219-2-3p in GC specimens with higher grade and later stage tumors. Meanwhile, miR-219-2-3p exerted antiproliferative, proapoptotic, and antimetastatic roles and reduced levels of p-ERK1/2 in GC cells. Furthermore, 5-aza-2'-deoxycytidine and trichostatin A increased the expression (~2 fold of miR-219-2-3p in GC cells. By methylation-specific PCR, DNA methylation in the upstream region of miR-219-2-3p was detected in both adjacent normal tissues and cancer tissues. As expected, the methylation level was considerably higher in the miR-219-2-3p down-regulated group than up-regulated group. CONCLUSIONS: miR-219-2-3p is potentially involved in gastric cancer progression and metastasis by regulating ERK1/2-related signal pathways, which may provide a novel therapeutic strategy

  1. Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore.

    Measday, Vivien; Hailey, Dale W; Pot, Isabelle; Givan, Scott A; Hyland, Katherine M; Cagney, Gerard; Fields, Stan; Davis, Trisha N; Hieter, Philip

    2002-01-01

    The budding yeast kinetochore is composed of an inner and outer protein complex, which binds to centromere (CEN) DNA and attaches to microtubules. We performed a genetic synthetic dosage lethality screen to identify novel kinetochore proteins in a collection of chromosome transmission fidelity mutants. Our screen identified several new kinetochore-related proteins including YLR381Wp/Ctf3p, which is a member of a conserved family of centromere-binding proteins. Ctf3p interacts with Mcm22p, Mcm16p, and the outer kinetochore protein Ctf19p. We used chromatin immunoprecipitation to demonstrate that Ctf3p, Mcm22p, and Mcm16p bind to CEN DNA in a Ctf19p-dependent manner. In addition, Ctf3p, Mcm22p, and Mcm16p have a localization pattern similar to other kinetochore proteins. The fission yeast Ctf3p homolog, Mis6, is required for loading of a CENP-A centromere specific histone, Cnp1, onto centromere DNA. We find however that Ctf3p is not required for loading of the budding yeast CENP-A homolog, Cse4p, onto CEN DNA. In contrast, Ctf3p and Ctf19p fail to bind properly to the centromere in a cse4-1 mutant strain. We conclude that the requirements for CENP-A loading onto centromere DNA differ in fission versus budding yeast. PMID:11782448

  2. Rhythmic expression of miR-27b-3p targets the clock gene Bmal1 at the posttranscriptional level in the mouse liver.

    Zhang, Wenxiang; Wang, Peng; Chen, Siyu; Zhang, Zhao; Liang, Tingming; Liu, Chang

    2016-06-01

    Circadian clocks orchestrate daily oscillations in mammalian behaviors, physiology, and gene expression. MicroRNAs (miRNAs) play a crucial role in fine-tuning of the circadian system. However, little is known about the direct regulation of the clock genes by specific miRNAs. In this study, we found that miR-27b-3p exhibits rhythmic expression in the metabolic tissues of the mice subjected to constant darkness. MiR-27b-3p's expression is induced in livers of unfed and ob/ob mice. In addition, the oscillation phases of miR-27b-3p can be reversed by restricted feeding, suggesting a role of peripheral clock in regulating its rhythmicity. Bioinformatics analysis indicated that aryl hydrocarbon receptor nuclear translocator-like (also known as Bmal1) may be a direct target of miR-27b-3p. Luciferase reporter assay showed that miR-27b-3p suppressed Bmal1 3' UTR activity in a dose-dependent manner, and mutagenesis of their binding site abolished this suppression. Furthermore, overexpression of miR-27b-3p dose-dependently reduced the protein expression levels of BMAL1 and impaired the endogenous BMAL1 and gluconeogenic protein rhythmicity. Collectively, our results suggest that miR-27b-3p plays an important role in the posttranscriptional regulation of BMAL1 protein in the liver. MiR-27b-3p may serve as a novel node to integrate the circadian clock and energy metabolism.-Zhang, W., Wang, P., Chen, S., Zhang, Z., Liang, T., Liu, C. Rhythmic expression of miR-27b-3p targets the clock gene Bmal1 at the posttranscriptional level in the mouse liver. PMID:26919869

  3. Produktion und Freisetzung von L-Glycerol-3-Phosphat (L-G3P) mit genetisch modifizierten Stämmen von Saccharomyces cerevisiae

    Popp, Almut

    2008-01-01

    L-G3P ist ein intrazelluläres phosphoryliertes Stoffwechselzwischenprodukt und zählte als solches bisher nicht zur Produktpalette von Hefe-Fermentationen. Um L-G3P als Baustein für die enzymatische Synthese von Monosacchariden und für andere Anwendungen zu gewinnen, wurden Bedingungen gesucht, unter denen ein S. cerevisiae-Produktionsstamm mit ausgeschalteter Glycerol-3-Phosphatase (gpp1/2) und Überexpression der cytosolischen Glycerol-3-Phosphat-Dehydrogenase (GPD1) gleichzeitig gute L-G3P-P...

  4. Measurement of the photoionization cross-section of the 3p{sup 2}P{sub 1/2,3/2} excited levels of sodium

    Amin, N.; Mahmood, S.; Anwar-ul-Haq, M.; Riaz, M.; Baig, M.A. [Quaid-i-Azam Univ., Atomic and Molecular Physics Lab., Dept. of Physics, Islamabad (Pakistan)

    2006-01-15

    The photoionization cross-section and number density of the 3p{sup 2}P(1/2,3/2) excited levels of sodium have been measured as a function of the laser energy using two-step laser excitation in conjunction with a thermionic diode working in the space charge limited mode. Employing the saturation technique, the cross-sections for the 3p{sup 2}P(1/2) and 3p{sup 2}P(3/2) levels are determined as 2.16 (43) Mb and 3.74 (74) Mb respectively. (authors)

  5. Electron-energy-loss spectroscopy of KxC60 and K-halides: comparison in the K-3p excitation region

    上野, 啓司; 斉木, 幸一朗; 小間, 篤

    2001-01-01

    We have investigated the electronic structure of KxC60 (x = 0-6) using low-energy electron energy loss spectroscopy (LEELS), especially focusing on the K-3p core-electron excitation spectra. It is found that the structure of the K-3p-excitation spectrum of KxC60 quite differs from that of KCI. Furthermore, the K-3p-excitation LEELS of K3C60 has been revealed to be different from that of K6C60. K-3p electrons are excited into K-4s- and K-3d-derived empty states in both KxC60 and KCI, but in th...

  6. Synthesis and Characterization of Organophosphazene Polyoxotungstate [(N3P3)(SiW11O39H2)3]12-

    Jian GONG; Chang Lu SHAO; Lun Yu QU

    2004-01-01

    A new organophosphazene polyoxotungstate, [(N3P3)(SiW11O39H2)3]12-, has been prepared by reaction of hexachlorocyclotriphosphazene with undecatungstosilicate, and characterized by elemental analysis, infrared spectroscopy, and multinuclear 31P NMR.

  7. T3P as an efficient cyclodehydration reagent for the one-pot synthesis of 2-amino-1,3,4-oxadiazoles

    Andivelu Ilangovan; Shanmugasundar Saravanakumar; Siddappa Umesh

    2015-05-01

    A scalable and environmentally friendly one-pot method for the synthesis of 2-amino-1,3,4-oxadiazoles from acylhydrazides and isocyanates has been achieved with propane phosponic anhydride (T3P) acting as cyclodehydrating reagent.

  8. Purification, crystallization and preliminary X-ray diffraction analysis of a soluble variant of the monoglyceride lipase Yju3p from the yeast Saccharomyces cerevisiae

    A soluble variant of the monoglyceride lipase Yju3p was successfully expressed, purified and crystallized. Diffraction data were collected to 2.4 Å resolution. The protein Yju3p is the orthologue of monoglyceride lipases in the yeast Saccharomyces cerevisiae. A soluble variant of this lipase termed s-Yju3p (38.3 kDa) was generated and purified to homogeneity by affinity and size-exclusion chromatography. s-Yju3p was crystallized in a vapour-diffusion setup at 293 K and a complete data set was collected to 2.4 Å resolution. The crystal form was orthorhombic (space group P212121), with unit-cell parameters a = 77.2, b = 108.6, c = 167.7 Å. The asymmetric unit contained four molecules with a solvent content of 46.4%

  9. Purification, crystallization and preliminary X-ray diffraction analysis of a soluble variant of the monoglyceride lipase Yju3p from the yeast Saccharomyces cerevisiae

    Rengachari, Srinivasan; Aschauer, Philipp; Sturm, Christian; Oberer, Monika, E-mail: m.oberer@uni-graz.at [University of Graz, Humboldtstrasse 50/3, 8010 Graz (Austria)

    2015-01-28

    A soluble variant of the monoglyceride lipase Yju3p was successfully expressed, purified and crystallized. Diffraction data were collected to 2.4 Å resolution. The protein Yju3p is the orthologue of monoglyceride lipases in the yeast Saccharomyces cerevisiae. A soluble variant of this lipase termed s-Yju3p (38.3 kDa) was generated and purified to homogeneity by affinity and size-exclusion chromatography. s-Yju3p was crystallized in a vapour-diffusion setup at 293 K and a complete data set was collected to 2.4 Å resolution. The crystal form was orthorhombic (space group P2{sub 1}2{sub 1}2{sub 1}), with unit-cell parameters a = 77.2, b = 108.6, c = 167.7 Å. The asymmetric unit contained four molecules with a solvent content of 46.4%.

  10. Measurement of electron-impact excitation into the 3p54p levels of argon using Fourier-transform spectroscopy

    To experimentally determine electron-impact excitation cross sections with the optical method, it is necessary to measure all transitions out of a level (the apparent cross sections), as well as the cascades into the level. In the case of the ten 3p54p levels of argon, the emissions to lower levels lie in the visible and near infrared (660 endash 1150 nm) and are hence observable with a monochromator endash photomultiplier-tube (PMT) system. A Fourier-transform spectrometer (FTS) allows us to measure the previously uninvestigated cascades that lie in the infrared. For the incident electron energy range between onset and 300 eV, we have measured the apparent cross sections with a monochromator-PMT system, and the cascade cross sections with a weak emission FTS system. The magnitude of both the apparent and cascade cross sections increases with target gas pressure due to radiation trapping effects. By subtracting the cascade contributions from the apparent cross sections, we have determined the direct cross sections and verified that they do not vary with pressure in the 0.5 endash 4-mTorr pressure range considered here. copyright 1998 The American Physical Society

  11. Preparation and H+ response mechanism of W/WO3 pH electrode by sol-gel method

    CHEN Dong-chu; FU Chao-yang; ZHEN Jia-sheng; WANG Zhe

    2005-01-01

    A method of sol-gel for preparing a W/WO3 pH electrode was presented. H+ response characteristics,such as response range, response sensitivity, response time were investigated. The effect of heat-treatment on response linear relation was discussed. The influences of interfered ions and solution temperatures were also taken into consideration. Many kinds of determination technology, such as TG, DSC, IR, SEM, AFM,XPS, XRD were used to characterize the film. The results show that the film appears in crack dried-mud, and the film is composed of ature; the W+6 in the electrode film transfers to W+5 in the course of H+ response; the H+ response course is controlled by the H+ diffusion from the solution to the WO3 crystalline, which is indicated in the EIS spectra; the best heat-treatment temperature is 200 ℃, and at this temperature, the electrode has an H+ response sensitivity of 52mV/pH.

  12. (2p/sup 2/)/sup 3/P state of H/sup -/ and the convergence of the CI series

    Jauregui, R.; Bunge, C.F.

    1979-12-01

    A sequence of increasingly accurate configuration interaction (CI) wave functions is used to discuss binding and the convergence of the CI series for the (2p/sup 2/)/sup 3/P state of H/sup -/. We get an upper bound energy E/sub u/=-0.1 253 547 166 a.u.(H), the lowest yet obtained, lying within an estimated 0.4 ..mu..hartree from the exact eigenvalue of Schroedinger's nonrelativistic equation. The angular energy limits define angular energy increments ..delta..E/sub l/ which follows the formula ..delta..E/sub l/approx. =0.18 (l+1/2)/sup -7/a.u. for l> or =4. Five or eighty per cent of binding is obtained with a three-term Psi=c/sub 1/(2p)/sup 2/+c/sub 2/(2p')/sup 2/+c/sub 3/ (3d)/sup 2/ depending on whether the 2p,2p', and 3d orbitals are energy optimized STO's or accurate natural orbitals, respectively.

  13. Kinetic isotope effect in the reaction of O(3P) with H2, D2, and HD

    The reaction of O(3P)+H2, D2, and HD was studied by the flash photolysis-resonance fluorescence method. The rate constants k/sub XY/(T) were measured over the temperature ranges 297--471 K for XY = H2, 422--472 K for D2, and 422--473 K for HD. Above 400 K k2 are in excellent agreement with earlier flow measurements, but below this temperature most of the flow data are systematically lower. A small deviation from the Arrhenius function was observed for k2 at 297 K. Our measurements of k2 are in excellent agreement with the results of Westenberg and deHaas and differ from those Clyne and Thrush. The measured values of k/sub HD/ equal the arithmetic mean of k2 and k2 within experimental error. A detailed comparison is made between the present results and the CEQB calculations of Bowman, Wagner, Walch, and Dunning and the ICVT/LAG calculations of Garrett and Truhlar. Good overall agreement is obtained with both theories

  14. Semirigid vibrating rotor target calculation for reaction O(3p)+CH4 →CH3+OH

    LIU; Xinguo; BAI; Lihua; ZHANG; Qinggang

    2004-01-01

    The time-dependent quantum dynamics calculation for reaction O(3p)+CH4→ CH3+OH is made, using of the semirigid vibrating rotor target (SVRT) model and the time-dependent wave packet (TDWP) method. The corresponding reaction probabilities of different initial states are provided. From the calculation of initial rovibrational state j= 0,v= 0, 1, we can see that the excitation of the H-CH3 stretching vibration gives significant enhancement of reaction probability and the reaction threshold decreases dramatically with the enhancement of the vibrating excitation, which indicates that the vibrating energy of reagent molecules contributes a lot to the molecular collision. As for the calculation of reaction probability of state v= 0, j= 0,1,2,3, the results show that the reaction probability rises significantly with the enhancement of rotational quantum number j while the reaction threshold has no changes. The spatial steric effect of the title reaction is studied and analyzed too after the calculation of reaction probability of states j= 5, k= 0-2, n= 0 and j=5, k=2, n=0-2 is made.

  15. SOX9 regulates microRNA miR-202-5p/3p expression during mouse testis differentiation

    Wainwright, Elanor N; Jorgensen, Joan S; Kim, Youngha; Truong, Vy; Bagheri-Fam, Stefan; Davidson, Tara; Svingen, Terje; Fernandez-Valverde, Selene L; McClelland, Kathryn S; Taft, Ryan J; Harley, Vincent R; Koopman, Peter; Wilhelm, Dagmar

    2013-01-01

    differentiation. These microRNAs are expressed in a sexually dimorphic pattern as the primordial XY gonad differentiates into a testis, with strong expression in Sertoli cells. In vivo, ectopic expression of pri-miR-202 in XX gonads did not result in molecular changes to the ovarian determination pathway...... promoter that is sufficient to drive expression in XY but not XX fetal gonads ex vivo. Mutation of SOX9 and SF1 binding sites reduced ex vivo transactivation of the pri-miR-202 promoter, demonstrating that pri-miR-202 may be a direct transcriptional target of SOX9/SF1 during testis differentiation. Our....... Expression of the primary transcript of miR-202-5p/3p remained low in XY gonads in a conditional Sox9-null mouse model, suggesting that pri-miR-202 transcription is downstream of SOX9, a transcription factor that is both necessary and sufficient for male sex determination. We identified the pri-miR-202...

  16. A common variant of PNPLA3 (p.I148M is not associated with alcoholic chronic pancreatitis.

    Jonas Rosendahl

    Full Text Available BACKGROUND: Chronic pancreatitis (CP is an inflammatory disease that in some patients leads to exocrine and endocrine dysfunction. In industrialized countries the most common aetiology is chronic alcohol abuse. Descriptions of associated genetic alterations in alcoholic CP are rare. However, a common PNPLA3 variant (p.I148M is associated with the development of alcoholic liver cirrhosis (ALC. Since, alcoholic CP and ALC share the same aetiology PNPLA3 variant (p.I148M possibly influences the development of alcoholic CP. METHODS: Using melting curve analysis we genotyped the variant in 1510 patients with pancreatitis or liver disease (961 German and Dutch alcoholic CP patients, 414 German patients with idiopathic or hereditary CP, and 135 patients with ALC. In addition, we included in total 2781 healthy controls in the study. RESULTS: The previously published overrepresentation of GG-genotype was replicated in our cohort of ALC (p-value <0.0001, OR 2.3, 95% CI 1.6-3.3. Distributions of genotype and allele frequencies of the p.I148M variant were comparable in patients with alcoholic CP, idiopathic and hereditary CP and in healthy controls. CONCLUSIONS: The absence of an association of PNPLA3 p.I148M with alcoholic CP seems not to point to a common pathway in the development of alcoholic CP and alcoholic liver cirrhosis.

  17. Analysis of a ribonuclease H digestion of N3'-->P5' phosphoramidate-RNA duplexes by capillary gel electrophoresis.

    DeDionisio, L; Gryaznov, S M

    1995-07-01

    Phosphodiester oligonucleotides (ODNs) and their analogs are presently being investigated as potential antisense therapeutics in the treatment of viral infections and various forms of cancer. here, we would like to report results from an investigation of activity for a ribonuclease H (RNase H) mediated RNA digestion assay in the duplexes formed by an ODN or the ODN analog, N3'-->P5' phosphoramidate (3'-phosphoramidate), and complimentary RNA strands. Capillary gel electrophoresis (CGE) proved to be an effective method for determining RNA hydrolysis in the presence of RNase H. RNA and an ODN or RNA and a 3'-phosphoramidate were hybridized in a Tris-HCl, MgCl2 buffer at room temperature (RT) and incubated with RNase H. Digestions were carried out at RT or at 37 degrees C. Control samples were unhybridized RNA with RNase H, RNA without RNase H, and duplexes (RNA-ODN or 3'-phosphoramidate) without RNase H. All controls were incubated in Tris-HCl, MgCl2 buffer, and sample aliquots were analyzed at various time intervals. A homodecamer, (dT)10, was used as an internal standard to determine the relative migration time of the RNA strand. The final digestion products for the duplexes and the various controls were monitored by CGE. In addition, polyacrylamide gel electrophoresis (PAGE) was used in conjunction with Stains-All (staining) and a densitometric analysis to verify CGE results. PMID:7581876

  18. Remote frequency measurement of the 1S0-3P1 transition in laser cooled 24Mg

    Friebe, J; Wübbena, T; Pape, A; Kelkar, H; Ertmer, W; Terra, O; Sterr, U; Weyers, S; Grosche, G; Schnatz, H; Rasel, E M

    2011-01-01

    We perform Ramsey-Borde spectroscopy on laser cooled magnesium atoms in free fall to measure the 1S0-3P1 intercombination transition frequency. The measured value of 655 659 923 839 730 (48) Hz is consistent with our former atomic beam measurement. We improve upon the fractional accuracy of the previous measurement by more than an order of magnitude to 7 x 10^-14. The magnesium frequency standard was referenced to a fountain clock of the Physikalisch Technische Bundesanstalt (PTB) via a phase-stabilized telecom fiber link and its stability characterized for interrogation times up to 8000 s. Our measurement revealed a new systematic effect due to the movement of atoms across the spectroscopy beams. A remarkable property of this effect is the counterintuitive reduction of residual Doppler shift with increasing resolution. Our theoretical model of the atom-light interaction is in agreement with the observed effect and allows us to quantify its contribution in the uncertainty budget.

  19. Formation of deeply bound ultracold Sr_2 molecules by photoassociation near the ^1S + ^3P_1 intercombination line

    Skomorowski, Wojciech; Koch, Christiane P

    2012-01-01

    We predict feasibility of the photoassociative formation of Sr_2 molecules in arbitrary vibrational levels of the electronic ground state based on state-of-the-art ab initio calculations. Key is the strong spin-orbit interaction between the c^3\\Pi_u, A^1\\Sigma_u^+ and B^1\\Sigma_u^+ states. It creates not only an effective dipole moment allowing free-to-bound transitions near the ^1S + ^3P_1 intercombination line but also facilitates bound-to-bound transitions via resonantly coupled excited state rovibrational levels to deeply bound rovibrational levels of the ground X^1\\Sigma_g^+ potential, with v" as low as v"=6. The spin-orbit interaction is responsible for both optical pathways. Therefore, those excited state levels that have the largest bound-to-bound transition moments to deeply bound ground state levels also exhibit a sufficient photoassociation probability, comparable to that of the lowest weakly bound excited state level previously observed by Zelevinsky et al. [Phys. Rev. Lett. 96, 203201 (2006)]. Ou...

  20. Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores

    King, Emma M J; Rachidi, Najma; Morrice, Nick; Hardwick, Kevin G.; Stark, Michael J. R.

    2007-01-01

    The spindle checkpoint delays anaphase onset until all chromosomes are correctly attached to microtubules. Ipl1 protein kinase (Aurora B) is required to correct inappropriate kinetochore-microtubule attachments and for the response to lack of tension between sister kinetochores. Here we identify residues in the checkpoint protein Mad3p that are phosphorylated by Ipl1p. When phosphorylation of Mad3p at two sites is prevented, the cell's response to reduced kinetochore tension is dramatically c...

  1. Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores

    King, Emma M J; Rachidi, Najma; Morrice, Nick; Hardwick, Kevin G.; Stark, Michael J. R.

    2007-01-01

    The spindle checkpoint delays anaphase onset until all chromosomes are correctly attached to microtubules. Ipl1 protein kinase (Aurora B) is required to correct inappropriate kinetochore–microtubule attachments and for the response to lack of tension between sister kinetochores. Here we identify residues in the checkpoint protein Mad3p that are phosphorylated by Ipl1p. When phosphorylation of Mad3p at two sites is prevented, the cell’s response to reduced kinetochore tension is dramatically c...

  2. A mixed-valent molybdenum monophosphate with a layer structure: KMo[sub 3]P[sub 2]O[sub 14

    Guesdon, A.; Borel, M.M.; Leclaire, A.; Grandin, A.; Raveau, B. (Universite de Caen (France))

    1994-03-01

    A new mixed-valent molybdenum monophosphate with a layer structure KMo[sub 3]P[sub 2]O[sub 14] has been isolated. It crystallizes in the space group P2[sub 1]/m with a = 8.599 (2) [angstrom], b = 6.392(2) [angstrom], c = 10.602(1) [angstrom], and [beta] = 111.65 (2)[degrees]. The layers [KMo[sub 3]P[sub 2]O[sub 14

  3. miR-181b-3p promotes epithelial-mesenchymal transition in breast cancer cells through Snail stabilization by directly targeting YWHAG.

    Yoo, Je-Ok; Kwak, Seo-Young; An, Hyun-Ju; Bae, In-Hwa; Park, Myung-Jin; Han, Young-Hoon

    2016-07-01

    Epithelial-mesenchymal transition (EMT) is essential for increased invasion and metastasis during cancer progression. Among the candidate EMT-regulating microRNAs that we previously identified, miR-181b-3p was found to induce EMT in MCF7 breast cancer cells, as indicated by an EMT-characteristic morphological change, increased invasiveness, and altered expression of an EMT marker. Transfection with a miR-181b-3p inhibitor reduced the expression of mesenchymal markers and the migration and invasion of highly invasive breast cancer cells. miR-181b-3p induced the upregulation of Snail, a master EMT inducer and transcriptional repressor of E-cadherin, through protein stabilization. YWHAG was identified as a direct target of miR-181b-3p, downregulation of which induced Snail stabilization and EMT phenotypes. Ectopic expression of YWHAG abrogated the effect of miR-181b-3p, including Snail stabilization and the promotion of invasion. In situ hybridization and immunohistochemical analyses indicated that YWHAG expression was inversely correlated with the expression of miR-181b-3p and Snail in human breast cancer tissues. Furthermore, transfection with miR-181b-3p increased the frequency of metastatic nodule formation in the lungs of mice in experimental metastasis assays using MDA-MB-231 cells. Taken together, our data suggest that miR-181b-3p functions as a metastasis activator by promoting Snail-induced EMT, and may therefore be a therapeutic target in metastatic cancers. PMID:27102539

  4. Epigenetic silencing of miR-490-3p promotes development of an aggressive colorectal cancer phenotype through activation of the Wnt/β-catenin signaling pathway.

    Zheng, Kehong; Zhou, Xinying; Yu, Jinlong; Li, Qiang; Wang, Hui; Li, Mingyi; Shao, Ziyun; Zhang, Feifei; Luo, Yuhao; Shen, Zetao; Chen, Fei; Shi, Fujun; Cui, Chunhui; Zhao, Dachuan; Lin, Zhiqun; Zheng, Wei; Zou, Zhaowei; Huang, Zonghai; Zhao, Liang

    2016-06-28

    The Wnt/β-catenin pathway is known to contribute to colorectal cancer (CRC) progression, although little is known about the contribution of β-catenin on this process. We investigated the role of miR-490-3p, which was recently reported to suppress tumorigenesis through its effect on Wnt/β-catenin signaling. We found that hypermethylation of the miR-490-3p promoter down-regulates miR-490-3p expression in CRC tissue. Gain- and loss-of-function assays in vitro and in vivo reveal that miR-490-3p suppresses cancer cell proliferation by inducing apoptosis and inhibits cell invasiveness by repressing the initiation of epithelial-to-mesenchymal transition (EMT), a key mechanism in cancer cell invasiveness and metastasis. The frequently rearranged in advanced T-cell lymphomas (FRAT1) protein was identified as a direct target of miR-490-3p and contributes to its tumor-suppressing effects. miR-490-3p appears to have an inhibitory effect on β-catenin expression in nuclear fractions of CRC cells, whereas FRAT1 expression is associated with the accumulation of β-catenin in the nucleus of cells, which could be weakened by transfection with miR-490-3p. Our findings suggest that the miR-490-3p/FRAT1/β-catenin axis is important in CRC progression and provides new insight into the molecular mechanisms underlying CRC. They may help to confirm the pathway driving CRC aggressiveness and serve for the development of a novel miRNA-targeting anticancer therapy. PMID:27037061

  5. Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore

    Measday, Vivien; Hailey, Dale W.; Pot, Isabelle; Givan, Scott A.; Hyland, Katherine M.; Cagney, Gerard; Fields, Stan; Davis, Trisha N.; Hieter, Philip

    2002-01-01

    The budding yeast kinetochore is composed of an inner and outer protein complex, which binds to centromere (CEN) DNA and attaches to microtubules. We performed a genetic synthetic dosage lethality screen to identify novel kinetochore proteins in a collection of chromosome transmission fidelity mutants. Our screen identified several new kinetochore-related proteins including YLR381Wp/Ctf3p, which is a member of a conserved family of centromere-binding proteins. Ctf3p interacts with Mcm22p, Mcm...

  6. MiR-132-3p Regulates the Osteogenic Differentiation of Thoracic Ligamentum Flavum Cells by Inhibiting Multiple Osteogenesis-Related Genes

    Qu, Xiaochen; Chen, Zhongqiang; Fan, Dongwei; Sun, Chuiguo; Zeng, Yan

    2016-01-01

    Ossification of the ligamentum flavum (OLF) is a disorder of heterotopic ossification of spinal ligaments and is the main cause of thoracic spinal canal stenosis. Previous studies suggested that miR-132-3p negatively regulates osteoblast differentiation. However, whether miR-132-3p is involved in the process of OLF has not been investigated. In this study, we investigated the effect of miR-132-3p and its target genes forkhead box O1 (FOXO1), growth differentiation factor 5 (GDF5) and SRY-box 6 (SOX6) on the osteogenic differentiation of ligamentum flavum (LF) cells. We demonstrated that miR-132-3p was down-regulated during the osteogenic differentiation of LF cells and negatively regulated the osteoblast differentiation. Further, miR-132-3p targeted FOXO1, GDF5 and SOX6 and down-regulated the protein expression of these genes. Meanwhile, FOXO1, GDF5 and SOX6 were up-regulated after osteogenic differentiation and the down-regulation of endogenous FOXO1, GDF5 or SOX6 suppressed the osteogenic differentiation of LF cells. In addition, we also found FOXO1, GDF5 and SOX6 expression in the ossification front of OLF samples. Overall, these results suggest that miR-132-3p inhibits the osteogenic differentiation of LF cells by targeting FOXO1, GDF5 and SOX6. PMID:27556448

  7. Aberrantly expressed miR-582-3p maintains lung cancer stem cell-like traits by activating Wnt/β-catenin signalling.

    Fang, Lishan; Cai, Junchao; Chen, Baixue; Wu, Shanshan; Li, Rong; Xu, Xiaonan; Yang, Yi; Guan, Hongyu; Zhu, Xun; Zhang, Le; Yuan, Jie; Wu, Jueheng; Li, Mengfeng

    2015-01-01

    Cancer stem cells (CSCs) are involved in tumorigenesis, tumour recurrence and therapy resistance and Wnt signalling is essential for the development of the biological traits of CSCs. In non-small cell lung carcinoma (NSCLC), unlike in colon cancer, mutations in β-catenin and APC genes are uncommon; thus, the mechanism underlying the constitutive activation of Wnt signalling in NSCLC remains unclear. Here we report that miR-582-3p expression correlates with the overall- and recurrence-free-survival of NSCLC patients, and miR-582-3p has an activating effect on Wnt/β-catenin signalling. miR-582-3p overexpression simultaneously targets multiple negative regulators of the Wnt/β-catenin pathway, namely, AXIN2, DKK3 and SFRP1. Consequently, miR-582-3p promotes CSC traits of NSCLC cells in vitro and tumorigenesis and tumour recurrence in vivo. Antagonizing miR-582-3p potently inhibits tumour initiation and progression in xenografted animal models. These findings suggest that miR-582-3p mediates the constitutive activation of Wnt/β-catenin signalling, likely serving as a potential therapeutic target for NSCLC. PMID:26468775

  8. Acidic phospholipid-independent interaction of Yas3p, an Opi1-family transcriptional repressor of Yarrowia lipolytica, with the endoplasmic reticulum.

    Kobayashi, Satoshi; Tezaki, Satoshi; Horiuchi, Hiroyuki; Fukuda, Ryouichi; Ohta, Akinori

    2015-12-01

    In the n-alkane-assimilating yeast Yarrowia lipolytica, the transcription of ALK1, encoding cytochrome P450, that catalyses n-alkane hydroxylation is activated by a complex composed of Yas1p and Yas2p via a promoter element, ARE1, in response to n-alkanes. An Opi1-family transcription factor, Yas3p, represses the transcription by binding to Yas2p in the nucleus when cultured in glucose-containing medium, but it is localized to the ER, presumably through interaction with acidic phospholipids, phosphatidic acid and/or phospho inositides, when cultured in n-alkane-containing medium. Here, to elucidate the mechanisms regulating the localization of Yas3p, point and deletion mutants of Yas3p were constructed and analysed. The substitution of Trp(360) and Cys(361) by Arg abrogated the localization of Yas3p to the ER and decreased ARE1-mediated transcriptional activation by n-alkane. A Yas3p truncation mutant consisting of residues 259-422 did not bind to acidic phospholipids, but it was localized to the ER in the presence of n-alkane, implying the acidic-phospholipid-independent recruitment of this mutant to the ER in response to n-alkane. The W360R and C361R substitutions in this truncation mutant abolished its localization to the ER. The results suggest that these residues are implicated in the acidic phospholipid-independent interaction of Yas3p to the ER. PMID:26284565

  9. miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1

    Qian, Tian-mei; Zhao, Li-li; Wang, Jing; Li, Ping; Qin, Jing; Liu, Yi-sheng; Yu, Bin; Ding, Fei; Gu, Xiao-song; Zhou, Song-lin

    2016-01-01

    MicroRNAs (miRNAs) are small, non-coding RNAs that negatively adjust gene expression in multifarious biological processes. However, the regulatory effects of miRNAs on Schwann cells remain poorly understood. Previous microarray analysis results have shown that miRNA expression is altered following sciatic nerve transaction, thereby affecting proliferation and migration of Schwann cells. This study investigated whether miR-148b-3p could regulate migration of Schwann cells by directly targeting cullin-associated and neddylation-dissociated 1 (Cand1). Up-regulated expression of miR-148b-3p promoted Schwann cell migration, whereas silencing of miR-148b-3p inhibited Schwann cell migration in vitro. Further experiments confirmed that Cand1 was a direct target of miR-148b-3p, and Cand1 knockdown reversed suppression of the miR-148b-3p inhibitor on Schwann cell migration. These results suggested that miR-148b-3p promoted migration of Schwann cells by directly targeting Cand1 in vitro.

  10. Human Cytomegalovirus miR-UL112-3p Targets TLR2 and Modulates the TLR2/IRAK1/NFκB Signaling Pathway.

    Igor Landais

    2015-05-01

    Full Text Available Human Cytomegalovirus (HCMV encodes multiple microRNAs (miRNAs whose functions are just beginning to be uncovered. Using in silico approaches, we identified the Toll-Like Receptor (TLR innate immunity pathway as a possible target of HCMV miRNAs. Luciferase reporter assay screens further identified TLR2 as a target of HCMV miR-UL112-3p. TLR2 plays a major role in innate immune response by detecting both bacterial and viral ligands, including HCMV envelope proteins gB and gH. TLR2 activates a variety of signal transduction routes including the NFκB pathway. Furthermore, TLR2 plays an important role in controlling CMV infection both in humans and in mice. Immunoblot analysis of cells transfected with a miR-UL112-3p mimic revealed that endogenous TLR2 is down-regulated by miR-UL112-3p with similar efficiency as a TLR2-targeting siRNA (siTLR2. We next found that TLR2 protein level decreases at late times during HCMV infection and correlates with miR-UL112-3p accumulation in fibroblasts and monocytic THP1 cells. Confirming direct miR-UL112-3p targeting, down-regulation of endogenous TLR2 was not observed in cells infected with HCMV mutants deficient in miR-UL112-3p expression, but transfection of miR-UL112-3p in these cells restored TLR2 down-regulation. Using a NFκB reporter cell line, we found that miR-UL112-3p transfection significantly inhibited NFκB-dependent luciferase activity with similar efficiency as siTLR2. Consistent with this observation, miR-UL112-3p transfection significantly reduced the expression of multiple cytokines (IL-1β, IL-6 and IL-8 upon stimulation with a TLR2 agonist. Finally, miR-UL112-3p transfection significantly inhibited the TLR2-induced post-translational activation of IRAK1, a kinase located in the upstream section of the TLR2/NFκB signaling axis. To our knowledge, this is the first identified mechanism of TLR2 modulation by HCMV and is the first report of functional targeting of TLR2 by a viral miRNA. These

  11. Synthesis, crystal structures and optical properties of two congruent-melting isotypic diphosphates: LiM3P2O7 (M=Na, K)

    Two new isotypic diphosphates, LiNa3P2O7 (1) and LiK3P2O7 (2), have been synthesized by conventional solid-state reaction. The single-crystal X-ray structural analyses have shown that they crystallize in the orthorhombic space group C2221 (No. 20) with the unit cells: a=5.4966(2) Å, b=9.1365(4) Å, c=12.2764(5) Å for compound 1 and a=6.0373(14) Å, b=9.339(2) Å, c=13.292(3) Å for compound 2. The LiM3P2O7 (M=Na, K) consist of two-dimensional [LiP2O7]3− layers, which are composed by LiO4 tetrahedral and diphosphate groups, the Na or K atoms are filled in the interlayers and balance the charge. Second harmonic generation (SHG) on powder samples have been measured using Kurtz and Perry techniques. Thermal analyses, IR spectroscopy, UV–vis–NIR diffuse reflectance spectra, and band structure calculations are performed on the reported compounds. - Graphical Abstract: LiM3P2O7 (M=Na, K) consists of a two-dimensional infinite [LiP2O7]3− layer, which is composed by LiO4 tetrahedra and diphosphate groups. Highlights: ► LiNa3P2O7 and LiK3P2O7 are new compounds in the Li2O–M2O (M=Na, K)–P2O5 systems. ► Crystal structures of LiNa3P2O7 and LiK3P2O7 consist of two-dimensional [LiP2O7]3− layers. ► LiNa3P2O7 and LiK3P2O7 are congruent melting compounds.

  12. Novel Antiplatelet Activity of Minocycline Involves Inhibition of MLK3-p38 Mitogen Activated Protein Kinase Axis.

    Joseph W Jackson

    Full Text Available Platelets play an essential role in hemostasis and wound healing by facilitating thrombus formation at sites of injury. Platelets also mediate inflammation and contain several pro-inflammatory molecules including cytokines and chemokines that mediate leukocyte recruitment and activation. Not surprisingly, platelet dysfunction is known to contribute to several inflammatory disorders. Antiplatelet therapies, such as aspirin, adenosine diphosphate (ADP antagonists, glycoprotein IIb/IIIa (GPIIb/IIIa inhibitors, and anticoagulants such as warfarin, dampen platelet activity at the risk of unwarranted bleeding. Thus, the development of drugs that reduce platelet-mediated inflammation without interfering with thrombus formation is of importance to combat platelet-associated disorders. We have shown here for the first time that the tetracycline antibiotic, minocycline, administered to HIV-infected individuals reduces plasma levels of soluble CD40L and platelet factor 4 levels, host molecules predominately released by platelets. Minocycline reduced the activation of isolated platelets in the presence of the potent platelet activator, thrombin, as measured by ELISA and flow cytometry. Platelet degranulation was reduced upon exposure to minocycline as shown by mepacrine retention and flow cytometry. However, minocycline had no effect on spreading, aggregation, GPIIb/IIIa activation, or in vivo thrombus formation. Lastly, immunoblot analysis suggests that the antiplatelet activity of minocycline is likely mediated by inhibition of mixed lineage kinase 3 (MLK3-p38 MAPK signaling axis and loss of p38 activity. Our findings provide a better understanding of platelet biology and a novel repurposing of an established antibiotic, minocycline, to specifically reduce platelet granule release without affecting thrombosis, which may yield insights in generating novel, specific antiplatelet therapies.

  13. Novel Antiplatelet Activity of Minocycline Involves Inhibition of MLK3-p38 Mitogen Activated Protein Kinase Axis

    Jackson, Joseph W.; Singh, Meera V.; Singh, Vir B.; Jones, Letitia D.; Davidson, Gregory A.; Ture, Sara; Morrell, Craig N.; Schifitto, Giovanni; Maggirwar, Sanjay B.

    2016-01-01

    Platelets play an essential role in hemostasis and wound healing by facilitating thrombus formation at sites of injury. Platelets also mediate inflammation and contain several pro-inflammatory molecules including cytokines and chemokines that mediate leukocyte recruitment and activation. Not surprisingly, platelet dysfunction is known to contribute to several inflammatory disorders. Antiplatelet therapies, such as aspirin, adenosine diphosphate (ADP) antagonists, glycoprotein IIb/IIIa (GPIIb/IIIa) inhibitors, and anticoagulants such as warfarin, dampen platelet activity at the risk of unwarranted bleeding. Thus, the development of drugs that reduce platelet-mediated inflammation without interfering with thrombus formation is of importance to combat platelet-associated disorders. We have shown here for the first time that the tetracycline antibiotic, minocycline, administered to HIV-infected individuals reduces plasma levels of soluble CD40L and platelet factor 4 levels, host molecules predominately released by platelets. Minocycline reduced the activation of isolated platelets in the presence of the potent platelet activator, thrombin, as measured by ELISA and flow cytometry. Platelet degranulation was reduced upon exposure to minocycline as shown by mepacrine retention and flow cytometry. However, minocycline had no effect on spreading, aggregation, GPIIb/IIIa activation, or in vivo thrombus formation. Lastly, immunoblot analysis suggests that the antiplatelet activity of minocycline is likely mediated by inhibition of mixed lineage kinase 3 (MLK3)-p38 MAPK signaling axis and loss of p38 activity. Our findings provide a better understanding of platelet biology and a novel repurposing of an established antibiotic, minocycline, to specifically reduce platelet granule release without affecting thrombosis, which may yield insights in generating novel, specific antiplatelet therapies. PMID:27270236

  14. Valence Band Character of NiS2-xSex using 3p-3d Resonant ARPES

    Han, Garam; Kim, Yeongkwan; Koh, Yoonyoung; Kim, Beomyoung; Song, Dongjoon; Seo, Jungjin; Kyung, Wonshik; Lee, Kyungdong; Kim, Changyoung

    2013-03-01

    Understanding the strong correlated system is one of the most challenging tasks in condensed matter physics. Especially, the metal insulator transition (MIT) has been one of the major topics recent few decades. NiS2-xSex is known as one of famous material which has MIT. The cubic pyrite NiS2 is a charge-transfer (CT) insulator. NiS2 attracts particular interest as it easily forms a solid solution with NiSe2 (NiS2-xSex) which, while being isoelectronic and isostructural to NiS2, is nevertheless a good metal. MIT, induced by Se alloying, is observed at low temperature (T) for x =0.45. Perucchi and his collaborators revealed closed relation between MIT and band width through comparison of infrared spectroscopy result and LDA calculation. However, it was only an indirect observation, and is inconsistent with recent proposal that NiS2 is not a CT insulator but an insulator due to the bonding-antibonding splitting in the S - S (Se - Se) dimers. To reveal the true mechanism in the MIT in NiS2-xSex, resonant photoemission experiment is essential. According to competing theories (CT insulator and insulator due to bonding-antibonding splitting), it is expected that the character of the main band that is responsible for the MIT should be different. Therefore, we performed 3p->3d resonant ARPES for various Se dopings (x =0.43; insulator, x =0.5, 0.7, 2.0; metal) and observed a significant change between on- and off-resonances near the MIT. Our experimental result supports that the origin of MIT in NiS2-xSex is the CT theory rather than the dimer theory.

  15. Effect of P5+ on spectroscopy and structure of Yb3+/Al3+/P5+ co-doped silica glass

    Two series of silica glasses [(95.9−x)SiO2–4Al2O3–xP2O5–0.1Yb2O3 (in mol%, x=0–10) and (92−y) SiO2–4Al2O3–4P2O5–yYb2O3 (in mol%, y=0–0.2)] were prepared by sol–gel method combined with high-temperature sintering. The relationship between the glass structure and spectroscopic properties is investigated. Significant alterations in density, refractive index, absorption and emission cross sections, fluorescence lifetime, and scalar crystal field NJ are observed at a molar ratio of P5+/Al3+=1 due to the transformation of P5+ structure type from AlPO4-like to PO4 units. This structural change is clearly observed in Raman spectra. In addition, NMR experimental results suggest that Yb3+ is mainly located in AlPO4-like but not SiO4 units at a molar ratio of P5+/Al3+=1, and also Raman spectra reveals the presence of P=O linkage in samples with molar ratio of P5+/Al3+>1. This further demonstrates that it is due to the formation of P=O site but not the simple addition of P2O5 in Yb3+/Al3+/P5+ co-doped silica glass to significantly decrease the absorption and emission cross sections of Yb3+. It reflects in the change of Yb3+ Stark splitting, and is revealed by the decline of Yb3+ asymmetry degree. - Highlights: • Silica glasses doped with P5+, Yb3+, Al3+ were successfully prepared. • Their optical and spectroscopic properties were measured at room temperature. • Structure–property relationships of these glasses were investigated

  16. Chicken gga-miR-103-3p Targets CCNE1 and TFDP2 and Inhibits MDCC-MSB1 Cell Migration.

    Han, Bo; Lian, Ling; Li, Xin; Zhao, Chunfang; Qu, Lujiang; Liu, Changjun; Song, Jiuzhou; Yang, Ning

    2016-01-01

    Marek's disease (MD) is a highly contagious viral neoplastic disease caused by Marek's disease virus (MDV), which can lead to huge economic losses in the poultry industry. Recently, microRNAs (miRNAs) have been found in various cancers and tumors. In recent years, 994 mature miRNAs have been identified through deep sequencing in chickens, but only a few miRNAs have been investigated further in terms of their function. Previously, gga-miR-103-3p was found downregulated in MDV-infected samples by using Solexa deep sequencing. In this study, we further verified the expression of gga-miR-103-3p among MDV-infected spleen, MD lymphoma from liver, noninfected spleen, and noninfected liver, by qPCR. The results showed that the expression of gga-miR-103-3p was decreased in MDV-infected tissues, which was consistent with our previous study. Furthermore, two target genes of gga-miR-103-3p, cyclin E1 (CCNE1) and transcription factor Dp-2 (E2F dimerization partner 2) (TFDP2), were predicted and validated by luciferase reporter assay, qPCR, and western blot analysis. The results suggested that CCNE1 and TFDP2 are direct targets of gga-miR-103-3p in chickens. Subsequent cell proliferation and migration assay showed that gga-miR-103-3p suppressed MDCC-MSB1 migration, but did not obviously modulate MDCC-MSB1 cell proliferation. In conclusion, gga-miR-103-3p targets the CCNE1 and TFDP2 genes, and suppresses cell migration, which indicates that it might play an important role in MD tumor transformation. PMID:26935418

  17. Blockage of miR-92a-3p with locked nucleic acid induces apoptosis and prevents cell proliferation in human acute megakaryoblastic leukemia.

    Sharifi, M; Salehi, R

    2016-01-01

    MicroRNAs (miRNAs) are non-coding RNAs involved in post-transcriptional regulation of gene expression. In many cancers, up- or downregulation of different miRNAs is reported. In acute myeloid leukemia, upregulation of miR-92a-3p was reported in human in vitro studies. We performed blockage of miR-29a-3p in human acute megakaryoblastic leukemia cell line (M-07e) by using locked nucleic acid (LNA) and cell proliferation; apoptosis and necrosis were assessed. At different time points after LNA-anti-miR92a-3p transfection, miR-92a-3p quantitation was assessed by qRT-real-time PCR, MTT assay and annexin/propidium iodide staining were performed. The data were processed using the ANOVA test. At all three time points, the expression of miR-92a-3p was lower in the LNA-anti-miR group compared with the control groups. Cell viability between LNA-Anti-miR and the control group was statistically significant. Blockage of miR-92a-3p was associated with increment of the ratio of apoptotic cells in the LNA-anti-miR group was higher than the other group. The ratio of necrotic cells in the LNA-antimiR group was higher than the other groups. These assessments indicate that miR-92a-3p blockage can decrease the viability of M-07e cells, which is mainly due to induction of apoptosis and necrosis. Our findings could open up a path to a miRNA based therapeutic approach for treatment of acute megakaryoblastic leukemia. PMID:26658357

  18. MicroRNA-193a-3p Reduces Intestinal Inflammation in Response to Microbiota via Down-regulation of Colonic PepT1.

    Dai, Xin; Chen, Xi; Chen, Qun; Shi, Lei; Liang, Hongwei; Zhou, Zhen; Liu, Qian; Pang, Wenjing; Hou, Dongxia; Wang, Cheng; Zen, Ke; Yuan, Yaozong; Zhang, Chen-Yu; Xia, Lu

    2015-06-26

    Intestinal inflammation is characterized by epithelial disruption, leading to the loss of barrier function, recruitment of immune cells, and host immune responses to gut microbiota. PepT1, a di/tripeptide transporter that uptakes bacterial products, is up-regulated in inflamed colon tissue, which implies its role in bacterium-associated intestinal inflammation. Although microRNA (miRNA)-mediated gene regulation has been found to be involved in various processes of inflammatory bowel disease (IBD), the biological function of miRNAs in the pathogenesis of IBD remains to be explored. In this study we detected miRNA expression patterns in colon tissues during colitis and investigated the mechanism underlying the regulation of colonic PepT1 by miRNAs. We observed an inverse correlation between PepT1 and miR-193a-3p in inflamed colon tissues with active ulcerative colitis, and we further demonstrated that miR-193a-3p reduced PepT1 expression and activity as a target gene and subsequently suppressed the NF-κB pathway. Intracolonic delivery of miR-193a-3p significantly ameliorated dextran sodium sulfate-induced colitis, whereas the overexpression of colonic PepT1 via PepT1 3'-untranslated region mutant lentivirus vector abolished the anti-inflammatory effect of miR-193a-3p. Furthermore, antibiotic treatment eliminated the difference in the dextran sodium sulfate-induced inflammation between the presence and absence of miR-193a-3p. These findings suggest that miR-193a-3p regulation of PepT1 mediates the uptake of bacterial products and is a potent mechanism during the colonic inflammation process. Overall, we believe miR-193a-3p may be a potent regulator of colonic PepT1 for maintaining intestinal homeostasis. PMID:25931122

  19. MicroRNA-331-3p Suppresses Cervical Cancer Cell Proliferation and E6/E7 Expression by Targeting NRP2

    Fujii, Tomomi; Shimada, Keiji; Asano, Aya; Tatsumi, Yoshihiro; Yamaguchi, Naoko; Yamazaki, Masaharu; Konishi, Noboru

    2016-01-01

    Aberrant expression of microRNAs (miRNAs) is involved in the development and progression of various types of cancers. In this study, we investigated the role of miR-331-3p in cell proliferation and the expression of keratinocyte differentiation markers of uterine cervical cancer cells. Moreover, we evaluated whether neuropilin 2 (NRP2) are putative target molecules that regulate the human papillomavirus (HPV) related oncoproteins E6 and E7. Cell proliferation in the human cervical cancer cell lines SKG-II, HCS-2, and HeLa was assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay. Cellular apoptosis was measured using the TdT-mediated dUTP nick end labeling (TUNEL) and Annexin V assays. Quantitative RT-PCR was used to measure the messenger RNA (mRNA) expression of the NRP2, E6, E7, p63, and involucrin (IVL) genes. A functional assay for cell growth was performed using cell cycle analyses. Overexpression of miR-331-3p inhibited cell proliferation, and induced G2/M phase arrest and apoptosis in SKG-II, HCS-2 and HeLa cells. The luciferase reporter assay of the NRP2 3′-untranslated region revealed the direct regulation of NRP2 by miR-331-3p. Gene expression analyses using quantitative RT-PCR in SKG-II, HCS-2, and HeLa cells overexpressing miR-331-3p or suppressing NRP2 revealed down-regulation of E6, E7, and p63 mRNA and up-regulation of IVL mRNA. Moreover, miR-331-3p overexpression was suppressed NRP2 expression in protein level. We showed that miR-331-3p and NRP2 were key effectors of cell proliferation by regulating the cell cycle, apoptosis. NRP-2 also regulates the expression of E6/E7 and keratinocyte differentiation markers. Our findings suggest that miR-331-3p has an important role in regulating cervical cancer cell proliferation, and that miR-331-3p may contribute to keratinocyte differentiation through NRP2 suppression. miR-331-3p and NRP2 may contribute to anti-cancer effects

  20. Differential effects of miR-34c-3p and miR-34c-5p on SiHa cells proliferation apoptosis, migration and invasion

    Lopez, Jesus Adrian [Laboratorio de Terapia Genica, Departamento de Genetica y Biologia Molecular, CINVESTAV, Av. IPN 2508, Mexico 07360 D.F. (Mexico); Alvarez-Salas, Luis Marat, E-mail: lalvarez@cinvestav.mx [Laboratorio de Terapia Genica, Departamento de Genetica y Biologia Molecular, CINVESTAV, Av. IPN 2508, Mexico 07360 D.F. (Mexico)

    2011-06-10

    Highlights: {yields} In this study we examine miR-34c-3p and miR-34c-5p functions in SiHa cells. {yields} We study miRNA effect on cell proliferation, anchorage independent growth, apoptosis, cell motility and invasion. {yields} We find that miR-34c-3p and miR-34c-5p inhibition of proliferation and anchorage independent growth are exclusive to SiHa cells. {yields} miR-34c-3p induces apoptosis and inhibits cell motility and invasion in SiHa cells. {yields} In this study we conclude that miR-34c-3p functions as a tumor suppressor differ from miR-34c-5p. -- Abstract: MicroRNAs (miRNA) regulate expression of several genes associated with human cancer. Here, we analyzed the function of miR-34c, an effector of p53, in cervical carcinoma cells. Expression of either miR-34c-3p or miR-34c-5p mimics caused inhibition of cell proliferation in the HPV-containing SiHa cells but not in other cervical cells irrespective of tumorigenicity and HPV content. These results suggest that SiHa cells may lack of regulatory mechanisms for miR-34c. Monolayer proliferation results showed that miR-34c-3p produced a more pronounced inhibitory effect although both miRNAs caused inhibition of anchorage independent growth at similar extent. However, ectopic expression of pre-miR-34c-3p, but not pre-miR-34c-5p, caused S-phase arrest in SiHa cells triggering a strong dose-dependent apoptosis. A significant inhibition was observed only for miR-34c-3p on SiHa cells migration and invasion, therefore implying alternative regulatory pathways and targets. These results suggest differential tumor suppressor roles for miR-34c-3p and miR-34c-5p and provide new insights in the understanding of miRNA biology.

  1. Differential effects of miR-34c-3p and miR-34c-5p on SiHa cells proliferation apoptosis, migration and invasion

    Highlights: → In this study we examine miR-34c-3p and miR-34c-5p functions in SiHa cells. → We study miRNA effect on cell proliferation, anchorage independent growth, apoptosis, cell motility and invasion. → We find that miR-34c-3p and miR-34c-5p inhibition of proliferation and anchorage independent growth are exclusive to SiHa cells. → miR-34c-3p induces apoptosis and inhibits cell motility and invasion in SiHa cells. → In this study we conclude that miR-34c-3p functions as a tumor suppressor differ from miR-34c-5p. -- Abstract: MicroRNAs (miRNA) regulate expression of several genes associated with human cancer. Here, we analyzed the function of miR-34c, an effector of p53, in cervical carcinoma cells. Expression of either miR-34c-3p or miR-34c-5p mimics caused inhibition of cell proliferation in the HPV-containing SiHa cells but not in other cervical cells irrespective of tumorigenicity and HPV content. These results suggest that SiHa cells may lack of regulatory mechanisms for miR-34c. Monolayer proliferation results showed that miR-34c-3p produced a more pronounced inhibitory effect although both miRNAs caused inhibition of anchorage independent growth at similar extent. However, ectopic expression of pre-miR-34c-3p, but not pre-miR-34c-5p, caused S-phase arrest in SiHa cells triggering a strong dose-dependent apoptosis. A significant inhibition was observed only for miR-34c-3p on SiHa cells migration and invasion, therefore implying alternative regulatory pathways and targets. These results suggest differential tumor suppressor roles for miR-34c-3p and miR-34c-5p and provide new insights in the understanding of miRNA biology.

  2. Downregulation of microRNA-362-3p and microRNA-329 promotes tumor progression in human breast cancer.

    Kang, H; Kim, C; Lee, H; Rho, J G; Seo, J-W; Nam, J-W; Song, W K; Nam, S W; Kim, W; Lee, E K

    2016-03-01

    p130Cas regulates cancer progression by driving tyrosine receptor kinase signaling. Tight regulation of p130Cas expression is necessary for survival, apoptosis, and maintenance of cell motility in various cell types. Several studies revealed that transcriptional and post-translational control of p130Cas are important for maintenance of its expression and activity. To explore novel regulatory mechanisms of p130Cas expression, we studied the effect of microRNAs (miRs) on p130Cas expression in human breast cancer MCF7 cells. Here, we provide experimental evidence that miR-362-3p and miR-329 perform a tumor-suppressive function and their expression is downregulated in human breast cancer. miR-362-3p and miR-329 inhibited cellular proliferation, migration, and invasion, thereby suppressing tumor growth, by downregulating p130Cas. Ectopic expression of p130Cas attenuated the inhibitory effects of the two miRs on tumor progression. Relative expression levels of miR-362-3p/329 and p130Cas between normal and breast cancer correlated inversely; miR-362-3p/329 expression was decreased, whereas that of p130Cas increased in breast cancers. Furthermore, we showed that downregulation of miR-362-3p and miR-329 was caused by differential DNA methylation of miR genes. Enhanced DNA methylation (according to methylation-specific PCR) was responsible for downregulation of miR-362-3p and miR-329 in breast cancer. Taken together, these findings point to a novel role for miR-362-3p and miR-329 as tumor suppressors; the miR-362-3p/miR-329-p130Cas axis seemingly has a crucial role in breast cancer progression. Thus, modulation of miR-362-3p/miR-329 may be a novel therapeutic strategy against breast cancer. PMID:26337669

  3. Phosphate enrichment mechanism in CaO-SiO2-FeO-Fe2O3-P2O5 steelmaking slags with lower binary basicity

    Li, Jin-yan; Zhang, Mei; Guo, Min; Yang, Xue-min

    2016-05-01

    The addition of silica to steelmaking slags to decrease the binary basicity can promote phosphate enrichment in quenched slag samples. In this study, we experimentally investigated phosphate enrichment behavior in CaO-SiO2-FeO-Fe2O3-P2O5 slags with a P2O5 content of 5.00% and the binary basicity B ranging from 1.0 to 2.0, where the (%Fe t O)/(%CaO) mass percentage ratio was maintained at 0.955. The experimental results are explained by the defined enrichment degree R_{C_2 S - C_3 P} of solid solution 2CaO·SiO2-3CaO·P2O5 (C2S-C3P), where R_{C_2 S - C_3 P} is a component of the developed ion and molecule coexistence theory (IMCT)- N i model for calculating the mass action concentrations N i of structural units in the slags on the basis of the IMCT. The asymmetrically inverse V-shaped relation between phosphate enrichment and binary basicity B was observed to be correlated in the slags under applied two-stage cooling conditions. The maximum content of P2O5 in the C2S-C3P solid solution reached approximately 30.0% when the binary basicity B was controlled at 1.3.

  4. MiRNA-199a-3p Regulates C2C12 Myoblast Differentiation through IGF-1/AKT/mTOR Signal Pathway

    Long Jia

    2013-12-01

    Full Text Available MicroRNAs constitute a class of ~22-nucleotide non-coding RNAs. They modulate gene expression by associating with the 3' untranslated regions (3' UTRs of messenger RNAs (mRNAs. Although multiple miRNAs are known to be regulated during myoblast differentiation, their individual roles in muscle development are still not fully understood. In this study, we showed that miR-199a-3p was highly expressed in skeletal muscle and was induced during C2C12 myoblasts differentiation. We also identified and confirmed several genes of the IGF-1/AKT/mTOR signal pathway, including IGF-1, mTOR, and RPS6KA6, as important cellular targets of miR-199a-3p in myoblasts. Overexpression of miR-199a-3p partially blocked C2C12 myoblast differentiation and the activation of AKT/mTOR signal pathway, while interference of miR-199a-3p by antisense oligonucleotides promoted C2C12 differentiation and myotube hypertrophy. Thus, our studies have established miR-199a-3p as a potential regulator of myogenesis through the suppression of IGF-1/AKT/mTOR signal pathway.

  5. Reactive scattering of carbon atoms: the reaction dynamics of C(3P, 1D) +C2H2 and C2H4

    The reaction dynamics of C(3P, 1D) with acetylene and ethylene have been investigated by using the crossed beam technique with mass spectrometric detection and time-of-flight (TOF) analysis. The novel capability of generating continuous supersonic beams of carbon atoms by a radio-frequency discharge beam source is exploited. From angular and velocity distribution measurements, the primary reaction products are identified, their relative importance assessed, and their dynamics of formation characterized. While the reaction C(3P) + C2H2(X1Σg+) has been found to lead to C3H + H and C3(X1Σg+) + H2(X1Σg+) in comparable amount, the reaction C(3P) + C2H4(X1At) has been found to lead, predominantly, to H + C3H3 (propargylene). The dynamics of the C(1D) reactions are also characterized. The spin-forbidden H2 elimination channel in the reaction C(3P) + C2H2 is attributed to the occurrence of inter-system-crossing between the triplet and singlet manifold of the C3H2 potential energy surfaces. Interestingly, these findings provide evidence that the C(3P) + C2H2 reaction maybe the source of both C3H and C3 species detected in the extreme environment of dense interstellar clouds and outflows of carbon stars, as well as in combustion systems. (author)

  6. Down-regulation of eIF4GII by miR-520c-3p represses diffuse large B cell lymphoma development.

    Krystyna Mazan-Mamczarz

    2014-01-01

    Full Text Available Deregulation of the translational machinery is emerging as a critical contributor to cancer development. The contribution of microRNAs in translational gene control has been established however; the role of microRNAs in disrupting the cap-dependent translation regulation complex has not been previously described. Here, we established that elevated miR-520c-3p represses global translation, cell proliferation and initiates premature senescence in HeLa and DLBCL cells. Moreover, we demonstrate that miR-520c-3p directly targets translation initiation factor, eIF4GII mRNA and negatively regulates eIF4GII protein synthesis. miR-520c-3p overexpression diminishes cells colony formation and reduces tumor growth in a human xenograft mouse model. Consequently, downregulation of eIF4GII by siRNA decreases translation, cell proliferation and ability to form colonies, as well as induces cellular senescence. In vitro and in vivo findings were further validated in patient samples; DLBCL primary cells demonstrated low miR-520c-3p levels with reciprocally up-regulated eIF4GII protein expression. Our results provide evidence that the tumor suppressor effect of miR-520c-3p is mediated through repression of translation while inducing senescence and that eIF4GII is a key effector of this anti-tumor activity.

  7. Two aluminotriphosphates with closely related intersecting tunnel structures involving tetrahedral 'AlP' chains and layers: AAl3(P3O10)2, A=Rb, Cs

    Two new aluminotriphosphates, RbAl3(P3O10)2 and CsAl3(P3O10)2, were synthesized by solid-state reaction. They crystallize in non-centrosymmetric space groups: C2221 with a=9.8757(7)A, b=12.8854(10)A, c=11.9192(7)A (Z=4) for RbAl3(P3O10)2 and C2ce with a=10.0048(7)A, b=13.3008(10)A, c=12.1698(7)A (Z=4) for CsAl3(P3O10)2. Their 3D frameworks, built up of corner sharing P3O10 groups, AlO4 tetrahedra and AlO6 octahedra, exhibit several remarkable features. The AlO4 tetrahedra and P3O10 groups are directly associated through the corners, forming helical columns in the Rb-phase and 'helicoid' layers in the Cs-phase. The simultaneous presence of AlO4 and AlO6 species, rather rare in phosphates, leads to the formation of closely related [Al3P6O24]∞ layers in both structures, which differ by their stacking mode. The presence of intersecting tunnels running along and [001] directions, with Rb+ and Cs+ sitting at the intersections, shows the opened character of these two structures

  8. A Genotoxic Stress-Responsive miRNA, miR-574-3p, Delays Cell Growth by Suppressing the Enhancer of Rudimentary Homolog Gene in Vitro

    Ken-ichi Ishikawa

    2014-02-01

    Full Text Available MicroRNA (miRNA is a type of non-coding RNA that regulates the expression of its target genes by interacting with the complementary sequence of the target mRNA molecules. Recent evidence has shown that genotoxic stress induces miRNA expression, but the target genes involved and role in cellular responses remain unclear. We examined the role of miRNA in the cellular response to X-ray irradiation by studying the expression profiles of radio-responsive miRNAs and their target genes in cultured human cell lines. We found that expression of miR-574-3p was induced in the lung cancer cell line A549 by X-ray irradiation. Overexpression of miR-574-3p caused delayed growth in A549 cells. A predicted target site was detected in the 3'-untranslated region of the enhancer of the rudimentary homolog (ERH gene, and transfected cells showed an interaction between the luciferase reporter containing the target sequences and miR-574-3p. Overexpression of miR-574-3p suppressed ERH protein production and delayed cell growth. This delay was confirmed by knockdown of ERH expression. Our study suggests that miR-574-3p may contribute to the regulation of the cell cycle in response to X-ray irradiation via suppression of ERH protein production.

  9. Fasting induces a subcutaneous-to-visceral fat switch mediated by microRNA-149-3p and suppression of PRDM16.

    Ding, Hanying; Zheng, Shasha; Garcia-Ruiz, Daniel; Hou, Dongxia; Wei, Zhe; Liao, Zhicong; Li, Limin; Zhang, Yujing; Han, Xiao; Zen, Ke; Zhang, Chen-Yu; Li, Jing; Jiang, Xiaohong

    2016-01-01

    Visceral adiposity is strongly associated with metabolic disease risk, whereas subcutaneous adiposity is comparatively benign. However, their relative physiological importance in energy homeostasis remains unclear. Here, we show that after 24-h fasting, the subcutaneous adipose tissue of mice acquires key properties of visceral fat. During this fast-induced 'visceralization', upregulation of miR-149-3p directly targets PR domain containing 16 (PRDM16), a key coregulatory protein required for the 'browning' of white fat. In cultured inguinal preadipocytes, overexpression of miR-149-3p promotes a visceral-like switch during cell differentiation. Mice deficient in miR-149-3p display an increase in whole-body energy expenditure, with enhanced thermogenesis of inguinal fat. However, a visceral-like adipose phenotype is observed in inguinal depots overexpressing miR-149-3p. These results indicate that in addition to the capacity of 'browning' to defend against hypothermia during cold exposure, the subcutaneous adipose depot is also capable of 'whitening' to preserve energy during fasting, presumably to maintain energy balance, via miR-149-3p-mediated regulation of PRDM16. PMID:27240637

  10. Fasting induces a subcutaneous-to-visceral fat switch mediated by microRNA-149-3p and suppression of PRDM16

    Ding, Hanying; Zheng, Shasha; Garcia-Ruiz, Daniel; Hou, Dongxia; Wei, Zhe; Liao, Zhicong; Li, Limin; Zhang, Yujing; Han, Xiao; Zen, Ke; Zhang, Chen-Yu; Li, Jing; Jiang, Xiaohong

    2016-01-01

    Visceral adiposity is strongly associated with metabolic disease risk, whereas subcutaneous adiposity is comparatively benign. However, their relative physiological importance in energy homeostasis remains unclear. Here, we show that after 24-h fasting, the subcutaneous adipose tissue of mice acquires key properties of visceral fat. During this fast-induced ‘visceralization', upregulation of miR-149-3p directly targets PR domain containing 16 (PRDM16), a key coregulatory protein required for the ‘browning' of white fat. In cultured inguinal preadipocytes, overexpression of miR-149-3p promotes a visceral-like switch during cell differentiation. Mice deficient in miR-149-3p display an increase in whole-body energy expenditure, with enhanced thermogenesis of inguinal fat. However, a visceral-like adipose phenotype is observed in inguinal depots overexpressing miR-149-3p. These results indicate that in addition to the capacity of ‘browning' to defend against hypothermia during cold exposure, the subcutaneous adipose depot is also capable of ‘whitening' to preserve energy during fasting, presumably to maintain energy balance, via miR-149-3p-mediated regulation of PRDM16. PMID:27240637

  11. Zn/sub 3/P/sub 2/ as an improved semiconductor for photovoltaic devices. Final report, July 17, 1976-September 1, 1978

    Catalano, A.; Dalal, V.; Devaney, W.E.; Fagen, E.A.; Hall, R.B.; Masi, J.V.; Warfield, G.; Wyeth, N.C.

    1978-01-01

    The goal of this work was to evaluate the suitability of Zn/sub 3/P/sub 2/ as a potentially low cost, high conversion efficiency material for photovoltaic devices. The important results of the research are presented and discussed. The major accomplishments of this work are: (1) the development of a vapor transport method for the growth of large single crystals; (2) the development of two methods of thin film growth: vacuum evaporation and close space transport; (3) the determination of the optical constants of Zn/sub 3/P/sub 2/ including the indices of refraction, the optical absorption coefficient, and the ultra-violet to visible reflectivity spectra; (4) a determination of the factors which influence the electrical conductivity and how these relate to the defect chemistry of Zn/sub 3/P/sub 2/; (5) measurement of the barrier height of metal-Zn/sub 3/P/sub 2/ contacts and the development of a model which relates the barrier height to the properties of the metal-semiconductor interface; (6) measurement of the minority carrier diffusion length in Zn/sub 3/P/sub 2/; (7) the development of several single and double layer anti-reflection coatings; and (8) the development of Schottky barrier photovoltaic devices employing a grid device and transparent metal film design, with conversion efficiencies as high as 6.08% (total area) or 7.6% (active area).

  12. Determination of the effective radiative lifetimes of the 6 3P1 atomic mercury level in low-pressure mercury discharges

    Experiments are described in which low-pressure mercury, mercury-argon and mercury-krypton discharges were irradiated with a dye laser pulse at 365.5 nm, thus exciting mercury atoms from the metastable 6 3P2 level to the 6 3D2 level. The 6 3D2 level decays radiatively to the 6 P levels. By recording the time dependence of the overpopulation in the 6 3P1 and the 6 1P1 level at the fluorescence signals at 254 nm and 185 nm, respectively, the effective radiative lifetime of these levels were determined. The effective radiative lifetime of the 6 3P1 level was measured in the k0R regime 0.1-500. The 6 1P1 lifetime was determined for the following discharge conditions: tube diameter 10-36 mm, mercury density 7.1018-2.1021 m-3, and noble gas pressure 0, 130, 400 Pa

  13. Long-range interactions between a He($2 ^3S$) atom and a He($2 ^3P$) atom for like isotopes

    Zhang, J Y; Sadeghpour, H R; Vrinceanu, D; Yan, Z C; 10.1103/.73.022710

    2006-01-01

    For the interactions between a He($2 ^3S$) atom and a He($2 ^3P$) atom for like isotopes, we report calculations of the coefficients determining the potential energies at large internuclear separations. Using accurate variational wave functions in Hylleraas coordinates we evaluate the coefficient $C_{3}$ of the resonant dipole-dipole energy, and the van der Waals coefficients $C_{6}$, $C_{8}$, and $C_{10}$ of the energies arising from the mutual perturbations of instantaneous electric dipole, quadrupole, and octupole interactions. We establish definitive values including treatment of the finite nuclear mass for the ${}^3$He($2 ^3S$)--${}^3$He($2 ^3P$) and ${}^4$He($2 ^3S$)--${}^4$He($2 ^3P$) interactions.

  14. High-Fidelity RF Gun Simulations with the Parallel 3D Finite Element Particle-In-Cell Code Pic3P

    Candel, A; Kabel, A.; Lee, L.; Li, Z.; Limborg, C.; Ng, C.; Schussman, G.; Ko, K.; /SLAC

    2009-06-19

    SLAC's Advanced Computations Department (ACD) has developed the first parallel Finite Element 3D Particle-In-Cell (PIC) code, Pic3P, for simulations of RF guns and other space-charge dominated beam-cavity interactions. Pic3P solves the complete set of Maxwell-Lorentz equations and thus includes space charge, retardation and wakefield effects from first principles. Pic3P uses higher-order Finite Elementmethods on unstructured conformal meshes. A novel scheme for causal adaptive refinement and dynamic load balancing enable unprecedented simulation accuracy, aiding the design and operation of the next generation of accelerator facilities. Application to the Linac Coherent Light Source (LCLS) RF gun is presented.

  15. Self-supported Cu3P nanowire arrays as an integrated high-performance three-dimensional cathode for generating hydrogen from water.

    Tian, Jingqi; Liu, Qian; Cheng, Ningyan; Asiri, Abdullah M; Sun, Xuping

    2014-09-01

    Searching for inexpensive hydrogen evolution reaction (HER) electrocatalysts with high activity has attracted considerable research interest in the past years. Reported herein is the topotactic fabrication of self-supported Cu3 P nanowire arrays on commercial porous copper foam (Cu3 P NW/CF) from its Cu(OH)2 NW/CF precursor by a low-temperature phosphidation reaction. Remarkably, as an integrated three-dimensional hydrogen-evolving cathode operating in acidic electrolytes, Cu3 P NW/CF maintains its activity for at least 25 hours and exhibits an onset overpotential of 62 mV, a Tafel slope of 67 mV dec(-1) , and a Faradaic efficiency close to 100 %. Catalytic current density can approach 10 mA cm(-2) at an overpotential of 143 mV. PMID:25044801

  16. A quantum time-dependent wave-packet study of intersystem crossing effects in the O(3P0, 1, 2) + D2(v = 0, j = 0) reaction

    Zhao, Juan

    2013-04-01

    We investigated spin-orbit-induced intersystem crossing effects in the title reaction by the time-dependent wave-packet method combined with an extended split operator scheme. We performed non-adiabatic calculations of the fine-structure-resolved cross section and adiabatic calculations of integral cross section. The calculations are based on the potential energy surfaces of 3A' and the two degenerate 3A'' states [S. Rogers, D. Wang, A. Kuppermann, and S. Walch, J. Phys. Chem. A 104, 2308 (2000)], 10.1021/jp992985g, together with the spin-orbit coupling matrix [B. Maiti and G. C. Schatz, J. Chem. Phys. 119, 12360 (2003)], 10.1063/1.1623481 and singlet 1A' potential energy surface [J. Dobbyn and P. J. Knowles, Faraday Discuss. 110, 247 (1998)]. The results of the O(3P) + D2 are similar to those of the O(3P) + H2 reaction. The product spin state-resolved reaction cross section and the total reaction cross section both show that the adiabatic channel is dominant in all cases, and the non-adiabatic channels have cross sections of several orders of magnitude smaller than the adiabatic channels at high collision energy. Although the cross sections caused by the intersystem crossing effects in the O(3P) + D2 reaction are larger than those in the O(3P) + H2 reaction, the differences in non-adiabaticity between these two reaction systems are quite modest. Based on the results of the O(3P) + H2 reaction, we can predict that the influence of spin-orbit on the total reaction cross sections of the O(3P) + D2 reaction is also insignificant. However, these non-adiabatic effects can be reflected in the presence of some forward-scattering in the angular distribution for the OD product.

  17. Up-regulation of Serum MiR-130b-3p Level is Associated with Renal Damage in Early Lupus Nephritis

    Wang, Wanpeng; Mou, Shan; Wang, Ling; Zhang, Minfang; Shao, Xinghua; Fang, Wei; Lu, Renhua; Qi, Chaojun; Fan, Zhuping; Cao, Qin; Wang, Qin; Fang, Yan; Ni, Zhaohui

    2015-08-01

    Systemic lupus erythematosus (SLE) is a common but severe autoimmune systemic inflammatory disease. Lupus nephritis (LN) is a serious complication of SLE,affecting up to 70% of SLE patients. Circulating microRNAs (miRNA) are emerging as biomarkers for pathological conditions and play significant roles in intercellular communication. In present research, serum samples from healthy control, early and late stage LN patients were used to analyze the expression profile of miRNAs by microarray. Subsequent study demonstrated that miR-130b-3p in serum of patients with early stage LN were significantly up-regulated when compared with healthy controls. In addition,we have also observed that the expression of a large amount of circulating microRNAs significantly decreased in patients with late stage LN. The further analysis found that the expression of serum miR-130b-3p was positively correlated with 24-hour proteinuria and renal chronicity index in patients with early stage LN.Transfection of renal tubular cellline(HK-2)with miR-130b-3p mimics can promote epithelial-mesenchymal transition (EMT). The opposite effects were observed when transfected with miR-130b-3p inhibitors. MiR-130b-3p negatively regulated ERBB2IP expression by directly targeting the 3‧-UTR of ERBB2IP The circulating miR-130b-3p might serve as a biomarker and play an important role in renal damage in early stage LN patients.

  18. Cleft Lip/Palate, Short Stature, and Developmental Delay in a Boy with a 5.6-Mb Interstitial Deletion Involving 10p15.3p14

    Gamba, Bruno F.; Rosenberg, Carla; Costa, Silvia; Richieri-Costa, Antonio; Ribeiro-Bicudo, Lucilene A.

    2015-01-01

    The chromosome interval 10p15.3p14 harbors about a dozen genes. This region has been implicated in a few well-known human phenotypes, namely HDR syndrome (hypoparathyroidism, sensorineural deafness, and renal dysplasia) and DGS2 (DiGeorge syndrome 2), but a number of variable phenotypes have also been reported. Cleft lip/palate seems to be a very unusual finding within the clinical spectrum of patients with this deletion. Here, we report a male child born with short stature, cleft lip/palate, and feeding problems who was found to have a 5.6-Mb deletion at 10p15.3p14. PMID:25852446

  19. C-H and H-H Bond Activation via Ligand Dearomatization/Rearomatization of a PN3P-Rhodium(I) Complex

    Huang, Kuo-Wei

    2015-04-13

    A neutral complex PN3P-Rh(I)Cl (2) was prepared from a reaction of the PN3P pincer ligand (1) with [Rh(COD)Cl]2 (COD = 1,5-cyclooctadiene). Upon treatment with a suitable base, H–H and Csp2–H activation reactions can be achieved through the deprotonation/reprotonation of one of the N–H arms and dearomatization/rearomatization of the central pyridine ring with the oxidation state of Rh remaining I.

  20. Cyclones in the Mediterranean region: present and future climate scenarios derived from a general circulation model (HadAM3P

    Chr. Anagnostopoulou

    2006-01-01

    Full Text Available In this paper, an attempt is made to assess and evaluate the skill of the Hadley Center atmospheric General Circulation Model (HadAM3P in generating successfully the frequency and intensity of severe cyclones (<1000 hPa in the Mediterranean region. The cyclonic occurrence is studied in three regions of enhanced cyclonic activity: Gulf of Genoa, Southern Italy and Cyprus. It was found that the HadAM3P predicts a future decrease of the frequency of the severe cyclones at the SLP level, but the future cyclones will be more intense (deeper, especially at the 500 hPa level.

  1. Localisation of the human hSuv3p helicase in the mitochondrial matrix and its preferential unwinding of dsDNA

    Minczuk, M.; Piwowarski, J.; Papworth, M.A.; Awiszus, K.; Schalinski, S.; Dziembowski, A.; Dmochowska, A.; Bartnik, E; Tokatlidis, K; Stepien, P P; Borowski, P

    2002-01-01

    We characterised the human hSuv3p protein belonging to the family of NTPases/helicases. In yeast mitochondria the hSUV3 orthologue is a component of the degradosome complex and participates in mtRNA turnover and processing, while in Caenorhabditis elegans the hSUV3 orthologue is necessary for viability of early embryos. Using immunofluorescence analysis, an in vitro mitochondrial uptake assay and sub‐fractionation of human mitochondria we show hSuv3p to be a soluble protein localised in the m...

  2. STAT3, p-STAT3 and HIF-1α are associated with vasculogenic mimicry and impact on survival in gastric adenocarcinoma

    Song, Yan-Yan; SUN, LI-DAN; LIU, MIN-LI; LIU, ZHONG-LIANG; Chen, Fei; ZHANG, YING-ZHE; Zheng, Yan; Zhang, Jian-Ping

    2014-01-01

    Vasculogenic mimicry (VM) formation is important for invasion and metastasis of tumor cells in gastric adenocarcinoma (GAC). The present study aimed to investigate the association between signal transducer and activator of transcription-3 (STAT3), phosphor-STAT3 (p-STAT3), hypoxia-inducible factor-1α (HIF-1α) and VM formation in GAC, and discuss their clinical significance and correlation with the prognosis of patients with GAC. The expression levels of STAT3, p-STAT3, HIF-1α and VM were asse...

  3. MDR3 P-glycoprotein, a phosphatidylcholine translocase, transports several cytotoxic drugs and directly interacts with drags as judged by interference with nucleotide trapping

    Smith, A.J.; van Helvoort, A.; van Meer, G; Szabó, K.; Welker, E; Szakács, G; Váradi, A; Sarkadi, B.; Borst, P

    2000-01-01

    The human MDR3 gene is a member of the multidrug resistance (MDR) gene family. The MDR3 P-glycoprotein is a transmembrane protein that translocates phosphatidylcholine. The MDR1 P-glycoprotein related transports cytotoxic drugs. Its overexpression can make cells resistant to a variety of drugs. Attempts to show that MDR3 P-glycoprotein can cause MDR have been unsuccessful thus far. Here, we report an increased directional transport of several MDR1 P-glycoprotein substrates, such as digoxin, p...

  4. Microdeletion in distal 17p13.1

    Zeesman, Susan; Kjaergaard, Susanne; Hove, Hanne Buciek;

    2012-01-01

    by real-time PCR. These patients share remarkably similar clinical features including microcephaly, mild developmental delay, generalized joint laxity, and a body posture with knee and elbow flexion and hands held in midline. They have distinctive facial features which include long midface...

  5. 染色体5q31-32与银屑病相关性分析:易感位点确定但与SLC22A4和SLC22A5的单核苷酸多态性无关

    Friberg; C.; Bjoerck; K.; Nilsson; S.

    2006-01-01

    作者既往曾报道染色体5q上的一个区域可能是银屑病的易感区。这一富含细胞因子区也被认为是其他自身免疫性疾病或炎症性疾病如克隆氏病(CD)和类风湿性关节炎(RA)的易感区。已报道3个特异的单核苷酸多态性(SNPs)与RA和CD相关,这种多态性变化改变了2个有机阳离子转运体、溶质转运蛋白家族22成员4和5(SLC2A4和SLC2A5)的功能活性。本文分析了这些单核苷酸多态性与银屑病的相关性,并对此区域和另外31个微卫星标记进行了更紧密的连锁分析。作者未检测出3个待测的单核苷酸多态性与银屑病的任何相关性。.然而,连锁分析结果支持这一区域与银屑病的发病有关。作者在伴有关节病变的一组患者中检测到D5S436标记物的非参数连锁峰值为3.1。这一结果支持另一项由冰岛研究者进行的银屑病研究,该研究检测到D5S2090标记物优势评分的对数峰值为2.6,距D5S436仅2Mb。这一结果提示染色体5q32区的易感位点与关节型银屑病相关。

  6. MiRNA-362-3p induces cell cycle arrest through targeting of E2F1, USF2 and PTPN1 and is associated with recurrence of colorectal cancer

    Christensen, Lise Lotte; Tobiasen, Heidi; Holm, Anja; Schepeler, Troels; Ostenfeld, Marie Stampe; Thorsen, Kasper; Rasmussen, Mads Heilskov; Birkenkamp-Demtröder, Karin; Sieber, Oliver M; Gibbs, Peter; Lubinski, Jan; Lamy, Philippe; Laurberg, Søren; Øster, Bodil; Hansen, Kristian Qvist; Hagemann-Madsen, Rikke; Byskov, Kristina; Ørntoft, Torben Falck; Andersen, Claus Lindbjerg

    2013-01-01

    as potential miR-362-3p targets by mRNA profiling of HCT116 cells over-expressing miR-362-3p. Subsequently, these genes were confirmed as direct targets by Luciferase reporter assays and their knockdown in vitro phenocopied the effects of miR-362-3p over-expression. We conclude that miR-362-3p may be...

  7. A Poll about Children and Weight: Crunch Time during the American Work and School Week--3 P.M. to Bed. Summary

    Robert Wood Johnson Foundation, 2013

    2013-01-01

    Childhood obesity is a major public health challenge today, with complex roots interwoven into nearly every facet of American life. This poll addresses one narrow slice of this web: the challenges that families face during the "crunch time" of the work and school week, between 3 p.m. and the time children go to bed. Compared to the school day,…

  8. Reverse energy-pooling collisions: K(5D)+Na(3S)→K(4P)+Na(3P)

    The observation of a heteronuclear reverse energy pooling process is reported. The cross section for the collision processes K(5D)+Na(3S)→K(4P)+Na(3P) has been measured and compared to calculations carried out using a multicrossing Landau-Zener model. copyright 1997 The American Physical Society

  9. MicroRNA-450a-3p represses cell proliferation and regulates embryo development by regulating Bub1 expression in mouse.

    Min Luo

    Full Text Available Bub1 is a critical component of the spindle assembly checkpoint (SAC and closely linked to cell proliferation and differentiation. We previously found that spontaneous abortion embryos contained a low level of Bub1 protein but normal mRNA level, while the knockdown of Bub1 leads to abnormal numerical chromosomes in embryonic cells. Here, we investigated the mechanism through which governs the post-transcriptional regulation of Bub1 protein expression level. We first conducted bioinformatics analysis and identified eight putative miRNAs that may target Bub1. Luciferase reporter assay confirmed that miR-450a-3p can directly regulate Bub1 by binding to the 3'-untranslated region of Bub1 mRNA. We found that the overexpression of miR-450a-3p in mouse embryonic fibroblast (MEF cells down-regulated Bub1 protein level, repressed cell proliferation, increased apoptosis and restricted most cells in G1 phase of the cell cycle. Furthermore, when the fertilized eggs were microinjected with miR-450a-3p mimics, the cleavage of zygotes was effectively suppressed. Our results strongly suggest that an abnormally decreased Bub1 level regulated by miRNAs may be implicated in the pathogenesis of spontaneous miscarriage. Therefore, the blockade of miR-450a-3p may be explored as a novel therapeutic strategy for preventing spontaneous miscarriages.

  10. Ba3P5N10Br:Eu(2+): a natural-white-light single emitter with a zeolite structure type.

    Marchuk, Alexey; Schnick, Wolfgang

    2015-02-16

    Illumination sources based on phosphor-converted light emitting diode (pcLED) technology are nowadays of great relevance. In particular, illumination-grade pcLEDs are attracting increasing attention. Regarding this, the application of a single warm-white-emitting phosphor could be of great advantage. Herein, we report the synthesis of a novel nitridophosphate zeolite Ba3P5N10Br:Eu(2+). Upon excitation by near-UV light, natural-white-light luminescence was detected. The synthesis of Ba3P5N10Br:Eu(2+) was carried out using the multianvil technique. The crystal structure of Ba3P5N10Br:Eu(2+) was solved and refined by single-crystal X-ray diffraction analysis and confirmed by Rietveld refinement and FTIR spectroscopy. Furthermore, spectroscopic luminescence measurements were performed. Through the synthesis of Ba3P5N10Br:Eu(2+), we have shown the great potential of nitridophosphate zeolites to serve as high-performance luminescence materials. PMID:25573329

  11. MiR-139-3p is related to left ventricular hypertrophy and cardiomyocyte apoptosis in two-kidney one-clip hypertensive rats

    Yang Xiaomin

    2015-01-01

    Full Text Available MicroRNAs (miRNAs are important post-transcriptional regulators of gene expression in many physiological and pathological processes. Previous studies have reported the role of miR-139-3p in cancer. However, its specific roles and functions in the heart undergoing hypertrophy have yet to be fully elucidated. In the present study, a significant upregulation of miR-139-3p expression was demonstrated in the left ventricular myocardium of two-kidney one-clip (2K1C hypertensive rats using microarray and quantitative real-time PCR (qRT-PCR. Based on computational analysis, we observed that miR-139-3p can control the expression of mitogen-activated protein kinase 1 (MAPK1 as a target gene, which is essential for the induction of cardiac hypertrophy and cardiomyocyte apoptosis. This study provides first information that the highly expressed miR-139-3p might be closely involved in MAPK1-mediated cardiac hypertrophy and cardiomyocyte apoptotic processes in 2K1C rat.

  12. On the scent of asymmetrical dandelion dendrimers. Part I. Starting materials from the regiospecific aminolysis of N 3P 3Cl 6 by amino-alcohols

    Sournies, François; Zai, Karine; Vercruysse, Karine; Labarre, Marie-Christine; Labarre, Jean-François

    1997-07-01

    Starting materials for the design of asymmetrical dandelion dendrimers were synthesized upon the preliminary fixation of suitable amino-alcohols on hexachlorocyclotriphosphazene, N 3P 3Cl 6, as "blockers". Further linkage of diamines on solid supports leads to the expected asymmetrical moieties.

  13. Microarray based analysis of an inherited terminal 3p26.3 deletion, containing only the CHL1 gene, from a normal father to his two affected children

    Lerone Margherita

    2011-04-01

    Full Text Available Abstract Background terminal deletions of the distal portion of the short arm of chromosome 3 cause a rare contiguous gene disorder characterized by growth retardation, developmental delay, mental retardation, dysmorphisms, microcephaly and ptosis. The phenotype of individuals with deletions varies from normal to severe. It was suggested that a 1,5 Mb minimal terminal deletion including the two genes CRBN and CNTN4 is sufficient to cause the syndrome. In addition the CHL1 gene, mapping at 3p26.3 distally to CRBN and CNTN4, was proposed as candidate gene for a non specific mental retardation because of its high level of expression in the brain. Methods and Results we describe two affected siblings in which array-CGH analysis disclosed an identical discontinuous terminal 3p26.3 deletion spanning less than 1 Mb. The deletion was transmitted from their normal father and included only the CHL1 gene. The two brothers present microcephaly, light mental retardation, learning and language difficulties but not the typical phenotype manifestations described in 3p- syndrome. Conclusion a terminal 3p26.3 deletion including only the CHL1 gene is a very rare finding previously reported only in one family. The phenotype of the affected individuals in the two families is very similar and the deletion has been inherited from an apparently normal parent. As already described for others recurrent syndromes with variable phenotype, these findings are challenging in genetic counselling because of an evident variable penetrance.

  14. Replication Study and Meta-Analysis in European Samples Supports Association of the 3p21.1 Locus with Bipolar Disorder

    Vassos, Evangelos; Steinberg, Stacy; Cichon, Sven; Breen, Gerome; Sigurdsson, Engilbert; Andreassen, Ole A; Djurovic, Srdjan; Morken, Gunnar; Grigoroiu-Serbanescu, Maria; Diaconu, Carmen C; Czerski, Piotr M; Hauser, Joanna; Babadjanova, Gulja; Abramova, Lilia I; Mühleisen, Thomas W; Nöthen, Markus M; Rietschel, Marcella; McGuffin, Peter; Clair, David St; Gustafsson, Omar; Melle, Ingrid; Pietiläinen, Olli P H; Ruggeri, Mirella; Tosato, Sarah; Werge, Thomas; Ophoff, Roel A; Rujescu, Dan; Børglum, Anders D; Mors, Ole; Mortensen, Preben B; Demontis, Ditte; Hollegaard, Mads V; van Winkel, Ruud; Kenis, Gunter; De Hert, Marc; Réthelyi, János M; Bitter, István; Rubino, I Alex; Golimbet, Vera; Kiemeney, Lambertus A; van den Berg, Leonard H; Franke, Barbara; Jönsson, Erik G; Farmer, Anne; Stefansson, Hreinn; Stefansson, Kari; Collier, David A

    2012-01-01

    Common genetic polymorphisms at chromosome 3p21.1, including rs2251219 in polybromo 1 (PBRM1), have been implicated in susceptibility to bipolar affective disorder (BP) through genome-wide association studies. Subsequent studies have suggested that this is also a risk locus for other psychiatric ...... phenotypes, including major depression and schizophrenia....

  15. Down-regulation of BTG1 by miR-454-3p enhances cellular radiosensitivity in renal carcinoma cells

    B cell translocation gene 1 (BTG1) has long been recognized as a tumor suppressor gene. Recent reports demonstrated that BTG1 plays an important role in progression of cell cycle and is involved in cellular response to stressors. However, the microRNAs mediated regulatory mechanism of BTG1 expression has not been reported so far. MicroRNAs can effectively influence tumor radiosensitivity by preventing cell cycle progression, resulting in enhancement of the cytotoxicity of radiotherapy efficacy. This study aimed to demonstrating the effects of microRNAs on the BTG1 expression and cellular radiosensitivity. The human renal carcinoma 786-O cells were treated with 5 Gy of X-rays. Expressions of BTG1 gene and miR-454-3p, which was predicted to target BTG1 by software algorithm, were analyzed by quantitative polymerase chain reaction. Protein expressions were assessed by Western blot. Luciferase assays were used to quantify the interaction between BTG1 3′-untranslated region (3′-UTR) and miR-454-3p. The radiosensitivity was quantified by the assay of cell viability, colony formation and caspase-3 activity. The expression of the BTG1 gene in 786-O cells was significantly elevated after treatments with X-ray irradiation, DMSO, or serum starvation. The up-regulation of BTG1 after irradiation reduced cellular radiosensitivity as demonstrated by the enhanced cell viability and colony formation, as well as the repressed caspase-3 activity. In comparison, knock down of BTG1 by siRNA led to significantly enhanced cellular radiosensitivity. It was found that miR-454-3p can regulate the expression of BTG1 through a direct interaction with the 3′-UTR of BTG1 mRNA. Decreasing of its expression level correlates well with BTG1 up-regulation during X-ray irradiation. Particularly, we observed that over-expression of miR-454-3p by transfection inhibited the BTG1 expression and enhanced the radiosensitivity. In addition, cell cycle analysis showed that over-expression of miR-454-3p

  16. Molecular epidemiology and genetic evolution of the whole genome of G3P[8] human rotavirus in Wuhan, China, from 2000 through 2013.

    Yuan-Hong Wang

    Full Text Available Rotaviruses are a major etiologic agent of gastroenteritis in infants and young children worldwide. Since the latter of the 1990s, G3 human rotaviruses referred to as "new variant G3" have emerged and spread in China, being a dominant genotype until 2010, although their genomic evolution has not yet been well investigated.The complete genomes of 33 G3P[8] human rotavirus strains detected in Wuhan, China, from 2000 through 2013 were analyzed. Phylogenetic trees of concatenated sequences of all the RNA segments and individual genes were constructed together with published rotavirus sequences.Genotypes of 11 gene segments of all the 33 strains were assigned to G3-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1, belonging to Wa genogroup. Phylogenetic analysis of the concatenated full genome sequences indicated that all the modern G3P[8] strains were assigned to Cluster 2 containing only one clade of G3P[8] strains in the US detected in the 1970s, which was distinct from Cluster 1 comprising most of old G3P[8] strains. While main lineages of all the 11 gene segments persisted during the study period, different lineages appeared occasionally in RNA segments encoding VP1, VP4, VP6, and NSP1-NSP5, exhibiting various allele constellations. In contrast, only a single lineage was detected for VP7, VP2, and VP3 genes. Remarkable lineage shift was observed for NSP1 gene; lineage A1-2 emerged in 2007 and became dominant in 2008-2009 epidemic season, while lineage A1-1 persisted throughout the study period.Chinese G3P[8] rotavirus strains have evolved since 2000 by intra-genogroup reassortment with co-circulating strains, accumulating more reassorted genes over the years. This is the first large-scale whole genome-based study to assess the long-term evolution of common human rotaviruses (G3P[8] in an Asian country.

  17. Serum microRNAs; miR-30c-5p, miR-223-3p, miR-302c-3p and miR-17-5p could be used as novel non-invasive biomarkers for HCV-positive cirrhosis and hepatocellular carcinoma.

    Oksuz, Zehra; Serin, Mehmet Sami; Kaplan, Engin; Dogen, Aylin; Tezcan, Seda; Aslan, Gonul; Emekdas, Gurol; Sezgin, Orhan; Altintas, Engin; Tiftik, Eyup Naci

    2015-03-01

    Recently, serum miRNAs have been evolved as possible biomarkers for different diseases including hepatocellular carcinoma and other types of cancers. Investigating certain serum miRNAs as novel non-invasive markers for early detection of HCV-positive cirrhosis and hepatocellular carcinoma (HCC). The expression profiles of 58 miRNA were analyzed in patient's plasma of chronic hepatitis C (CHC), HCV-positive cirrhosis and HCV-positive HCC and compared with control group samples. Totally 94 plasma samples; 64 patient plasma (26 CHC, 30 HCV-positive cirrhosis, 8 HCV-positive HCC) and 28 control group plasma, were included. The expression profiles of 58 miRNAs were detected for all patient and control group plasma samples by qRT-PCR using BioMarkTM 96.96 Dynamic Array (Fluidigm Corporation) system. In CHC group, expression profiles of miR-30a-5p, miR-30c-5p, miR-206 and miR-302c-3p were found significantly deregulated (p < 0.05) when compared versus control group. In HCV-positive cirrhosis group, expression profiles of miR-30c-5p, miR-223-3p, miR-302c-3p, miR-17-5p, miR-130a-3p, miR-93-5p, miR-302c-5p and miR-223-3p were found significantly deregulated (p < 0.05). In HCV-positive HCC group, expression profiles of miR-17-5p, miR-223-3p and miR-24-3p were found significant (p < 0.05). When all groups were compared versus control, miR-30c-5p, miR-223-3p, miR-302c-3p and miR-17-5p were found significantly deregulated for cirrhosis and HCC. These results imply that miR-30c-5p, miR-223-3p, miR-302c-3p and miR-17-5p could be used as novel non-invasive biomarkers of HCV-positive HCC in very early, even at cirrhosis stage of liver disease. PMID:25391771

  18. Pressure-induced Pr{sup 3+} {sup 3}P{sub 0} luminescence in cubic Y{sub 2}O{sub 3}

    Srivastava, Alok M. [GE Global Research, One Research Circle, Niskayuna 12309, NY (United States); Renero-Lecuna, Carlos [MALTA CONSOLIDER Team – Dpto. Física Aplicada, Facultad de Ciencias, Universidad de Cantabria, Santander 39005 (Spain); Santamaría-Pérez, David [MALTA CONSOLIDER Team – Dpto. Químico Física, Facultad de Ciencias Químicas, Universidad Complutense Madrid, E-28040 Madrid (Spain); Rodríguez, Fernando [MALTA CONSOLIDER Team – DCITIMAC, Facultad de Ciencias, Universidad de Cantabria, Santander 39005 (Spain); Valiente, Rafael, E-mail: valientr@unican.es [MALTA CONSOLIDER Team – Dpto. Física Aplicada, Facultad de Ciencias, Universidad de Cantabria, Santander 39005 (Spain)

    2014-02-15

    An explanation for the puzzling absence of luminescence from the Pr{sup 3+} {sup 3}P{sub J[=0,1,2]} states in C-Ln{sub 2}O{sub 3} (cubic; Ln{sup 3+}=Lu{sup 3+}, Y{sup 3+}, Gd{sup 3+}) family of materials is provided by conducting a study of the emission properties of C-Y{sub 2}O{sub 3}:Pr{sup 3+} under applied hydrostatic pressure. Above 7 GPa, electronic transitions from the Pr{sup 3+} {sup 3}P{sub J[=0,1,2]} states are observed in the emission spectrum of C-Y{sub 2}O{sub 3}:Pr{sup 3+} at room temperature and below. The experimental data reveal that the crystal-field split Pr{sup 3+} 4f{sup 1}5d{sup 1} configuration is located entirely within the host lattice conduction band and that the promotion of the electron to the Pr{sup 3+} 4f{sup 1}5d{sup 1} state produces a self-trapped exciton-like state with the configuration, [Pr{sup 4+}+e{sub CB}], where e{sub CB} indicates an electron in the host lattice conduction band. Upon excitation, the exciton-like state bypasses the upper emitting {sup 3}P{sub J[=0,1,2]} states and directly feeds the lower emitting {sup 1}D{sub 2} state. This explains the absence of optical transitions from the Pr{sup 3+} {sup 3}P{sub J[=0,1,2]} states in the emission spectrum of C-Y{sub 2}O{sub 3}:Pr{sup 3+} at ambient pressure. At high pressures, emission transitions from the Pr{sup 3+} {sup 3}P{sub J[=0,1,2]} states are observed because of the localization of the Pr{sup 3+} 4f{sup 1}5d{sup 1} state to below the host lattice conduction band edge. -- Highlights: • Explanation for the complete absence of luminescence from the Pr{sup 3+} {sup 3}P{sub J} state in cubic Ln{sub 2}O{sub 3} sesquioxides. • By high pressure experiments, we have associated the absence of Pr{sup 3+} {sup 3}P{sub J} emission to the presence of an exciton state • The exciton state bypasses the upper emitting {sup 3}P{sub J} states and directly feeds the lower emitting {sup 1}D{sub 2} state. • Apart from the fundamental interest, this finding is relevantly

  19. Experimental investigation of the chemistry of exccited states of rare gases. Fourth quarterly (and annual) progress report, July 15, 1978-October 15, 1978. [Reaction of Ar(/sup 3/P/sub 2/) or Xe(/sup 3/P/sub 2/) with XeO/sub 4/

    Setser, D.W.

    1978-12-01

    A brief account is given of work on the quenching of XeF(B) and other excited rare-gas systems. A more detailed report is given of the studies of the vacuum uv photolysis of XeO/sub 4/ and reactions of Xe(/sup 3/P/sub 2/) and Ar(/sup 3/P/sub 2/) with XeO/sub 4/. (DLC)

  20. Repression of mitochondrial translation, respiration and a metabolic cycle-regulated gene, SLF1, by the yeast Pumilio-family protein Puf3p.

    Chatenay-Lapointe, Marc; Shadel, Gerald S

    2011-01-01

    Synthesis and assembly of the mitochondrial oxidative phosphorylation (OXPHOS) system requires genes located both in the nuclear and mitochondrial genomes, but how gene expression is coordinated between these two compartments is not fully understood. One level of control is through regulated expression mitochondrial ribosomal proteins and other factors required for mitochondrial translation and OXPHOS assembly, which are all products of nuclear genes that are subsequently imported into mitochondria. Interestingly, this cadre of genes in budding yeast has in common a 3'-UTR element that is bound by the Pumilio family protein, Puf3p, and is coordinately regulated under many conditions, including during the yeast metabolic cycle. Multiple functions have been assigned to Puf3p, including promoting mRNA degradation, localizing nucleus-encoded mitochondrial transcripts to the outer mitochondrial membrane, and facilitating mitochondria-cytoskeletal interactions and motility. Here we show that Puf3p has a general repressive effect on mitochondrial OXPHOS abundance, translation, and respiration that does not involve changes in overall mitochondrial biogenesis and largely independent of TORC1-mitochondrial signaling. We also identified the cytoplasmic translation factor Slf1p as yeast metabolic cycle-regulated gene that is repressed by Puf3p at the post-transcriptional level and promotes respiration and extension of yeast chronological life span when over-expressed. Altogether, these results should facilitate future studies on which of the many functions of Puf3p is most relevant for regulating mitochondrial gene expression and the role of nuclear-mitochondrial communication in aging and longevity. PMID:21655263

  1. Quantitative Proteomic Analysis of the Metastasis-Inhibitory Mechanism of miR-193a-3p in Non-Small Cell Lung Cancer

    Wei Deng

    2015-03-01

    Full Text Available Background: microRNAs can repress the expression of target genes by destabilizing their mRNAs or by inhibiting their translation. Our previous findings suggested that miR-193a-3p inhibited the progression of NSCLC both in vitro and in vivo. However, the biological processes and molecular pathways through which this miRNA exerts its positive effects are unknown. Methods: To explore the molecular mechanisms by which miR-193a-3p inhibited NSCLC metastasis, we investigated the changes in the protein profile of SPC-A-1sci (highly metastatic cells in response to the up-regulation of miR-193a-3p expression using a proteomics approach (iTRAQ combined with NanoLC-MS/MS. Changes in the profiles of the expressed proteins were verified using western blotting and were analyzed using the DAVID and STRING programs. Results: In the two replicated experiments, 4962/4946 proteins were identified, and the levels of expression of 4923/4902 proteins were quantified. In total, 112 of these proteins were differentially expressed. Among them, the up-regulated levels of expression of two of the 62 proteins with up-regulated expression (PPP2R2A and GSN and the down-regulated levels of expression four of the 50 proteins with down-regulated expression (LMNB2, UHRF1, G3BP1, and HNRNPU were verified using western blotting. The bioinformatics analysis revealed the interactions and signaling networks of these differentially expressed proteins. Conclusion: miR-193a-3p inhibited the metastasis of lung cancer cells by deregulating the expression of tumor-related proteins. These findings may improve the understanding of the molecular mechanisms underlying the metastatic-inhibitory effect of miR-193a-3p on lung cancer cells.

  2. miR-126-3p and miR-451a correlate with clinicopathological features of lung adenocarcinoma: The underlying molecular mechanisms.

    Chen, Qingyong; Hu, Huizhen; Jiao, Demin; Yan, Jie; Xu, Wei; Tang, Xiali; Chen, Jun; Wang, Jian

    2016-08-01

    Lung cancer is the most common malignancy worldwide. This study aimed to identify miRNA biomarkers of lung adenocarcinoma and to investigate their molecular mechanisms. miRNA expression profiling of tumor tissues and adjacent normal tissues from 10 patients were detected using microarray. Differentially expressed miRNAs (DEMs) were identified, and were verified using quantitative reverse transcription-PCR. Thereafter, correlations between DEM expression and clinicopathological features were determined in 49 patients. Furthermore, Targetscan was utilized to predict target genes, among which transcription factors (TFs) were identified. The interactions among miRNAs, TFs and target genes were used to construct an miRNA-TF-target network. Totally, 11 DEMs were identified, among which two downregulated miRNAs (miR-126-3p and miR-451a) were validated. Low levels of miR-126-3p and miR-451a were associated with poor pathological stage, large tumor diameter and lymph node metastasis (P0.65, P<0.05). For miR-126-3p, 154 target genes were predicted (e.g., PLXNB2), which were enriched in 29 pathways mainly concerning apoptosis and cancer. For miR‑451a, 397 target genes were predicted, which were enriched in 5 pathways including 'PPAR signaling pathway'. Ten genes were co-regulated by miR-126-3p and miR-451a, e.g., TSC1. Furthermore, an miRNA-TF-target network was constructed, and a sub-network was identified, including 2 miRNAs, 15 targets, and 7 TFs. In conclusion, miR-126-3p and miR-451a predicted the severity of lung adenocarcinoma. However, the possible mechanisms explored by bioinformatics need to be further validated. PMID:27277197

  3. Life cycle environmental impacts from CZTS (copper zinc tin sulfide) and Zn3P2 (zinc phosphide) thin film PV (photovoltaic) cells

    While solar PV (photovoltaic) cells are promising for clean energy production, their mass deployment is hindered by production costs, material availability, and toxicity. Two materials that can overcome these challenges and replace today's CdTe (cadmium telluride) and CIGS (copper indium gallium diselenide) based PV cells are Zn3P2 (zinc phosphide) and CZTS (copper zinc tin sulfide). A cradle to gate life cycle assessment was conducted to understand the environmental impacts from these technologies. The impacts from Zn3P2 and CdTe were similar and lower than the impacts from CZTS and CIGS. While CdTe has the toxic Cd element, the ecotoxicity impact from material acquisition and processing was higher for Zn and P than for CdTe. In CIGS, the ecotoxicity impact came mainly from Ga and would be significantly reduced if CZTS were to replace CIGS in the commercial market. For all four thin films studied, the contribution of raw materials to total impact was much lower than the impact coming from electricity consumption during the manufacturing stage. Therefore, to reduce environmental impact, future PV technology development should focus more on the process improvement. The manufacturing stages that contributed most to the impact were the absorber layer for CIGS and CZTS and the substrate cleaning for CdTe and Zn3P2. - Highlights: • Four impact categories were evaluated for CZTS, Zn3P2, and current PV technologies. • The analysis was cradle-to-gate and performed with GaBi 6.0 and Ecoinvent 2.2. • The impacts of Zn3P2 are similar to CdTe and lower than that of CIGS and CZTS. • Materials in the absorber layer did not usually have a large contribution. • Electricity use was a major contributor to the overall impact

  4. A potential regulatory role for intronic microRNA-338-3p for its host gene encoding apoptosis-associated tyrosine kinase.

    Aron Kos

    Full Text Available MicroRNAs (miRNAs are important gene regulators that are abundantly expressed in both the developing and adult mammalian brain. These non-coding gene transcripts are involved in post-transcriptional regulatory processes by binding to specific target mRNAs. Approximately one third of known miRNA genes are located within intronic regions of protein coding and non-coding regions, and previous studies have suggested a role for intronic miRNAs as negative feedback regulators of their host genes. In the present study, we monitored the dynamic gene expression changes of the intronic miR-338-3p and miR-338-5p and their host gene Apoptosis-associated Tyrosine Kinase (AATK during the maturation of rat hippocampal neurons. This revealed an uncorrelated expression pattern of mature miR-338 strands with their host gene. Sequence analysis of the 3' untranslated region (UTR of rat AATK mRNA revealed the presence of two putative binding sites for miR-338-3p. Thus, miR-338-3p may have the capacity to modulate AATK mRNA levels in neurons. Transfection of miR-338-3p mimics into rat B35 neuroblastoma cells resulted in a significant decrease of AATK mRNA levels, while the transfection of synthetic miR-338-5p mimics did not alter AATK levels. Our results point to a possible molecular mechanism by which miR-338-3p participates in the regulation of its host gene by modulating the levels of AATK mRNA, a kinase which plays a role during differentiation, apoptosis and possibly in neuronal degeneration.

  5. A Potential Regulatory Role for Intronic microRNA-338-3p for Its Host Gene Encoding Apoptosis-Associated Tyrosine Kinase

    Kos, Aron; Olde Loohuis, Nikkie F. M.; Wieczorek, Martha L.; Glennon, Jeffrey C.; Martens, Gerard J. M.; Kolk, Sharon M.; Aschrafi, Armaz

    2012-01-01

    MicroRNAs (miRNAs) are important gene regulators that are abundantly expressed in both the developing and adult mammalian brain. These non-coding gene transcripts are involved in post-transcriptional regulatory processes by binding to specific target mRNAs. Approximately one third of known miRNA genes are located within intronic regions of protein coding and non-coding regions, and previous studies have suggested a role for intronic miRNAs as negative feedback regulators of their host genes. In the present study, we monitored the dynamic gene expression changes of the intronic miR-338-3p and miR-338-5p and their host gene Apoptosis-associated Tyrosine Kinase (AATK) during the maturation of rat hippocampal neurons. This revealed an uncorrelated expression pattern of mature miR-338 strands with their host gene. Sequence analysis of the 3′ untranslated region (UTR) of rat AATK mRNA revealed the presence of two putative binding sites for miR-338-3p. Thus, miR-338-3p may have the capacity to modulate AATK mRNA levels in neurons. Transfection of miR-338-3p mimics into rat B35 neuroblastoma cells resulted in a significant decrease of AATK mRNA levels, while the transfection of synthetic miR-338-5p mimics did not alter AATK levels. Our results point to a possible molecular mechanism by which miR-338-3p participates in the regulation of its host gene by modulating the levels of AATK mRNA, a kinase which plays a role during differentiation, apoptosis and possibly in neuronal degeneration. PMID:22363537

  6. Repression of mitochondrial translation, respiration and a metabolic cycle-regulated gene, SLF1, by the yeast Pumilio-family protein Puf3p.

    Marc Chatenay-Lapointe

    Full Text Available Synthesis and assembly of the mitochondrial oxidative phosphorylation (OXPHOS system requires genes located both in the nuclear and mitochondrial genomes, but how gene expression is coordinated between these two compartments is not fully understood. One level of control is through regulated expression mitochondrial ribosomal proteins and other factors required for mitochondrial translation and OXPHOS assembly, which are all products of nuclear genes that are subsequently imported into mitochondria. Interestingly, this cadre of genes in budding yeast has in common a 3'-UTR element that is bound by the Pumilio family protein, Puf3p, and is coordinately regulated under many conditions, including during the yeast metabolic cycle. Multiple functions have been assigned to Puf3p, including promoting mRNA degradation, localizing nucleus-encoded mitochondrial transcripts to the outer mitochondrial membrane, and facilitating mitochondria-cytoskeletal interactions and motility. Here we show that Puf3p has a general repressive effect on mitochondrial OXPHOS abundance, translation, and respiration that does not involve changes in overall mitochondrial biogenesis and largely independent of TORC1-mitochondrial signaling. We also identified the cytoplasmic translation factor Slf1p as yeast metabolic cycle-regulated gene that is repressed by Puf3p at the post-transcriptional level and promotes respiration and extension of yeast chronological life span when over-expressed. Altogether, these results should facilitate future studies on which of the many functions of Puf3p is most relevant for regulating mitochondrial gene expression and the role of nuclear-mitochondrial communication in aging and longevity.

  7. Oncogenic Role of miR-15a-3p in 13q Amplicon-Driven Colorectal Adenoma-to-Carcinoma Progression.

    de Groen, Florence L M; Timmer, Lisette M; Menezes, Renee X; Diosdado, Begona; Hooijberg, Erik; Meijer, Gerrit A; Steenbergen, Renske D M; Carvalho, Beatriz

    2015-01-01

    Progression from colorectal adenoma to carcinoma is strongly associated with an accumulation of genomic alterations, including gain of chromosome 13. This gain affects the whole q arm and is present in 40%-60% of all colorectal cancers (CRCs). Several genes located at this amplicon are known to be overexpressed in carcinomas due to copy number dosage. A subset of these genes, including the mir-17~92 cluster, are functionally involved in CRC development. The present study set out to explore whether apart from mir-17~92, other miRNAs located at the 13q amplicon show a copy number dependent dosage effect that may contribute to 13q-driven colorectal adenoma-to-carcinoma progression. Integration of publically available miRNA expression, target mRNA expression and DNA copy number data from 125 CRCs yielded three miRNAs, miR-15a, -17, and -20a, of which high expression levels were significantly correlated with a 13q gain and which influenced target mRNA expression. These results could be confirmed by qRT-PCR in a series of 100 colon adenomas and carcinomas.Functional analysis of both mature miRNAs encoded by mir-15a, i.e. miR-15a-5p and miR-15a-3p, showed that silencing of miR-15a-3p significantly inhibited viability of CRC cells. Integration of miR-15a expression levels with mRNA expression data of predicted target genes identified mitochondrial uncoupling protein 2 (UCP2) and COP9 signalosome subunit 2 (COPS2) as candidates with significantly decreased expression in CRCs with 13q gain. Upon silencing of miR-15a-3p, mRNA expression of both genes increased in CRC cells, supporting miR-15a-3p mediated regulation of UPC2 and COPS2 expression. In conclusion, significant overexpression of miR-15a-3p due to gain of 13q is functionally relevant in CRC, with UCP2 and COPS2 as candidate target genes. Taken together our findings suggest that miR-15a-3p may contribute to adenoma-to-carcinoma progression. PMID:26148070

  8. Characterization of interface defects in ALD Al2O3/p-GaSb MOS capacitors using admittance measurements in range from kHz to GHz

    Gu, Siyuan; Min, Jie; Taur, Yuan; Asbeck, Peter M.

    2016-04-01

    Atomic layer deposited (ALD) Al2O3/p-type GaSb Metal-Oxide-Semiconductor (MOS) capacitors are studied with capacitance-voltage (C-V) and conductance-voltage (G-V) measurements using AC signal frequencies covering the range from kHz to GHz. The potential and limitations of the measurements at GHz frequencies for oxide and interface defect characterization are described. The effect of bulk oxide traps in communication with the GaSb valence band via hole tunneling is highlighted. Modeling indicates that the C-V and G-V frequency dispersions observed in the accumulation, flat-band and depletion regions of the Al2O3/p-GaSb MOS capacitors are due to combined contributions of bulk-oxide traps and interface traps.

  9. Calculation of the photo-electrons angular distribution asymmetry parameter β2p near the (3s3p)Ip resonance of He

    Near the (3s3p)1p resonance of He, we have calculated the photo-electrons angular distribution asymmetry parameter β2p in the diagonalization approximation. Using the measured value of βn=2 near the (3s3p)1p level obtained by Lindle et al. in the resonance photo-ionization of He to He+(n=2), we have estimated the ratio R=σ2p/σ2s of the partial 2p photo-ionization cross section to the partial 2s photo-ionization cross section. Our calculation supports the result that in the resonance region, the formation of ions in the 2p level dominates over the 2s level. This is in good agreement with the experimental and most of the theoretical results reported to date. (author). 18 refs, 1 fig., 2 tabs

  10. Programming of adipose tissue miR-483-3p and GDF-3 expression by maternal diet in type 2 diabetes

    Ferland-McCollough, D; Fernandez-Twinn, D S; Cannell, I G;

    2012-01-01

    Nutrition during early mammalian development permanently influences health of the adult, including increasing the risk of type 2 diabetes and coronary heart disease. However, the molecular mechanisms underlying such programming are poorly defined. Here we demonstrate that programmed changes in mi......-life nutrition, limits storage of lipids in adipose tissue, causing lipotoxicity and insulin resistance and thus increasing susceptibility to metabolic disease.......RNA expression link early-life nutrition to long-term health. Specifically, we show that miR-483-3p is upregulated in adipose tissue from low-birth-weight adult humans and prediabetic adult rats exposed to suboptimal nutrition in early life. We demonstrate that manipulation of miR-483-3p levels in vitro...

  11. By inhibiting snail signaling and miR-23a-3p, osthole suppresses the EMT-mediated metastatic ability in prostate cancer.

    Wen, Yu-Ching; Lee, Wei-Jiunn; Tan, Peng; Yang, Shun-Fa; Hsiao, Michael; Lee, Liang-Ming; Chien, Ming-Hsien

    2015-08-28

    Here we showed that Osthole, 7-methoxy-8-(3-methyl-2-butenyl) coumarin, a bioactive coumarin derivative extracted from medicinal plants, inhibited migration, invasion, epithelial to mesenchymal transition (EMT) in androgen-independent prostate cancer (AIPC) cells in vitro and metastasis of AIPC in vivo. In patients, high Snail levels were correlated with a higher histological Gleason sum and poor survival rates. Osthole inhibited the TGF-β/Akt/MAPK pathways, reduced Snail-DNA-binding activity and induced E-cadherin. We found that osthole decreased miR-23a-3p. Ectopic miR-23a-3p suppressed E-cadherin 3' untranslated region reporter activity and E-cadherin expression, and relieved the motility suppression caused by osthole treatment. PMID:26110567

  12. The synthesis of the heterocubane cluster [{CpMn}4(μ3-P)4] as a tetrahedral shaped starting material for the formation of polymeric coordination compounds.

    Heinl, Sebastian; Kiefer, Konrad; Balázs, Gábor; Wickleder, Claudia; Scheer, Manfred

    2015-09-11

    Thermolysis of [CpMn(η(6)-cht)] with P4 in 1,3-diisopropylbenzene leads to the formation of the heterocubane [Cp4Mn4P4] () in high yields, as a rare example of 'naked' phosphorus containing complexes of manganese. Compound is characterized and studied by DFT calculations and reflection measurements. 1D coordination polymers [{(CpMn)4(μ3-P)4}(CuX)]n (2-Cl: X = Cl; 2-Br: X = Br) are obtained in the reaction with CuX. Furthermore, it is shown that all four P atoms in can be addressed for a coordination towards cymantrene resulting in [{(CpMn)4(μ3-P)4}{CpMn(CO)2}n] (: n = 1; : n = 2; : n = 3; : n = 4), and shows that is a tetra-topic building block in coordination chemistry. PMID:26225545

  13. Electron-impact studies of atomic oxygen: II. Emission cross section measurements of the O I 3So→3P transition (130.4 nm)

    The optical excitation function of the O I 3So→3P transition (130.4 nm), produced by electron-impact excitation of atomic oxygen, has been measured over an extended energy range from threshold to 1.0 keV. Measurements were obtained in a crossed-beam experiment using both magnetically confined and electrostatically focused electrons in collision with atomic oxygen produced by a microwave discharge source. A 0.2 m vacuum ultraviolet monochromator system was used to measure the emitted O I radiation at 130.4 nm. The relative O I (130.4 nm) emission intensity corresponding to the 3So→3P transition was then put on the absolute scale by normalization to the O I (130.4 nm) cross section produced by dissociative excitation of O2 at 30 eV (Kanik et al 2000). (author)

  14. Towards a Mg lattice clock: Observation of the $^1S_{0}-$$^3P_{0}$ transition and determination of the magic wavelength

    Kulosa, A P; Zipfel, K H; Rühmann, S; Sauer, S; Jha, N; Gibble, K; Ertmer, W; Rasel, E M; Safronova, M S; Safronova, U I; Porsev, S G

    2015-01-01

    We optically excite the electronic state $3s3p~^3P_{0}$ in $^{24}$Mg atoms, laser-cooled and trapped in a magic-wavelength lattice. An applied magnetic field enhances the coupling of the light to the otherwise strictly forbidden transition. We determine the magic wavelength, the quadratic magnetic Zeeman shift and the transition frequency to be 468.463(207)$\\,$nm, -206.6(2.0)$\\,$MHz/T$^2$ and 655 058 646 691(101)$\\,$kHz, respectively. These are compared with theoretical predictions and results from complementary experiments. We also developed a high-precision relativistic structure model for magnesium, give an improved theoretical value for the blackbody radiation shift and discuss a clock based on bosonic magnesium.

  15. Influence of relaxation effects on probabilities of the 2s2p3 S2—2s2 2p2 3p1,2intercombination

    袁萍; 刘欣生; 等

    2003-01-01

    Transition probabilities of the 2s2p3 5S2-2s2 2P2 3P1,2 intercombination transitions in NII have been calculated by using a large-scale multiconfiguration Dirac-Fock method.In the calculation themost important effects of relativity,correlation,and relaxation are considered.From the calculated transition probabilities,the lifetime of the s22p3 5S2 metastable state is derived.The result is in excellent agreement agreement with the latest experimental result.In the meantime the influence of anomalously strong relaxation effects on probabilities of the 2s2p3 5S2-2s2 2p2 3P1,2 lines in NII have been found.

  16. Autosomal dominant congenital cataract in a Libyan Jewish family: cosegregation with a reciprocal chromosomal translocation [t(3;5)(p22.3; p15.1)

    Zafer, Emre; Meck, Jeanne; Gerrad, Liora; Pras, Elon; Frydman, Moshe; Reish, Orit; Avni, Isaac; Pras, Eran

    2008-01-01

    Purpose To describe a Jewish family of Libyan ancestry in which autosomal dominant congenital cataract segregates with an apparently balanced reciprocal chromosomal translocation. Methods Detailed family history and clinical data were recorded. Cytogenetic studies were performed on 13 family members. Results Embryonal cataracts cosegregated through three generations with a balanced chromosomal translocation [t(3;5)(p22.3; p15.1)] while the unbalanced translocation product, 46,XY,-5,+der(5)t(3...

  17. Zn/sub 3/P/sub 2/ as an improved semiconductor for photovoltaic solar cells. Ninth quarterly report, September 1, 1980-November 31, 1980

    None

    1980-01-01

    Frequency and voltage dependent capacitance measurements of ZnO/Zn/sub 3/P/sub 2/ heterojunctions have been made and the results interpreted according to a model which considers the total capacitance arising from contributions due to the depletion layer capacitance, interface charges and a dipole charge. The capacitance of the devices is anomalously high at low frequencies but agrees well with the value calculated from the bulk carrier density at 1 MHz. The data suggest a significant contribution to the capacitance from the charging and discharging of interface states. Direct evidence for a relationship between defects in the Zn/sub 3/P/sub 2/ (grains boundaries, dislocations, etc.,) and the collected current have been found by comparing EBIC images of ZnO/Zn/sub 3/P/sub 2/ devices with the etched surfaces of the samples. The series resistance of thin film Zn/sub 3/P/sub 2/ devices has been analyzed and the origin of the series resistance considered. A new substrate consisting of sequentially deposited layers of Fe and Si on mica has resulted in improved device performance. Devices with a conversion efficiency as high as 2.7% have been obtained. Fill factors in excess of 50% have been obtained for the first time. The diffusion coefficient of magnesium has been measured to 100/sup 0/C and 125/sup 0/C using a spectral response method. A model including contributions from both diffusion and space charge recombination terms was utilized to calculate the open circuit voltage and short circuit current as a function of junction depth of the n on p devices. A comparison of the experimental J/sub sc/ and V/sub oc/ as a function of junction depth was made by characterizing the devices after successive surface diffusions. Results are presented and disucssed. (WHK)

  18. RAMAN SPECTRUM STUDIES OF THE COORDINATIONS OF ALUMINIUM AND BORON IN Na2O-Al2O3-B2O3-P2O5 GLASSES

    Yifen, Yin; Dehua, Jiang; Xiangsheng, Chen; Beiya, Bian; Xihuai, Huang

    1985-01-01

    The coordination states of aluminium and boron in Na2O-Al2O3-B2O3-P2O5 glasses have been studied by means of Raman spectroscopy. The study shows that when boron and aluminium ions exist simultaneously in phosphate glasses, it is possible that boron ions form BO4 tetrahedra prior to aluminium ions, and the former has three and four coordinations while the latter has four and six.

  19. Electron correlation effects on the hyperfine induced 47Ti18+ (2s2p 3P0 → 2s21S0) transition probability

    We calculated the hyperfine induced transition probability for 47Ti18+ (2s2p 3P0 → 2s21S0) and analysed the influence of the electron correlation effects in order to interpret the reason for a large difference between existing experimental value and previous only one theoretical result with neglected electron correlation effects. An improved result AHFI=0.3708 s-1 was obtained. (author)

  20. Lifetime measurement of some excited states belonging to the 3p4nd ( = 4–6) configurationof ArII

    S Karmakar; M B Das

    2007-09-01

    The radiative lifetimes of eight levels belonging to the 3p4nd ( = 4–6) configuration of ArII have been measured using high frequency deflection technique together with a delayed coincidence single photon counting arrangement. Lifetimes of some of the levels have been measured for the first time. The results have been compared with other experimental and theoretical values.

  1. Performance Based Logistics (PBL) for the FA-18/S-3/P-3/C-2 auxiliary power unit (APU) at Honeywell: an applied analysis

    Landreth, Clifford J.; Corporon, Laura L.; Wilhelm, Richard H.

    2005-01-01

    The purpose of this MBA project is to evaluate and assess the metrics, incentives and other terms and conditions of the Performance Based Logistics (PBL) contract between Naval Aviation Inventory Control Point (NAVICP) and Honeywell in support of FA-18/S-3/P-3/C-2 Auxiliary Power Unit (APU) to determine if the contractual terms and conditions established are effective in facilitating and encouraging the full potential of PBL savings and improved performance. PBL is an acquisition reform initi...

  2. Low-dose irradiation promotes Rad51 expression by down-regulating miR-193b-3p in hepatocytes

    Lee, Eon-Seok; Won, Yeo Jin; Kim, Byoung-Chul; Park, Daeui; Bae, Jin-Han; Park, Seong-Joon; Noh, Sung Jin; Kang, Yeong-Rok; Choi, Si Ho; Yoon, Je-Hyun; Heo, Kyu; Yang, Kwangmo; Son, Tae Gen

    2016-05-01

    Current evidence indicates that there is a relationship between microRNA (miRNA)-mediated gene silencing and low-dose irradiation (LDIR) responses. Here, alterations of miRNA expression in response to LDIR exposure in male BALB/c mice and three different types of hepatocytes were investigated. The miRNome of the LDIR-exposed mouse spleens (0.01 Gy, 6.5 mGy/h) was analyzed, and the expression of miRNA and mRNA was validated by qRT-PCR. Western blotting, chromatin immunoprecipitation (ChIP), and luciferase assays were also performed to evaluate the interaction between miRNAs and their target genes and to gain insight into the regulation of miRNA expression. The expression of miRNA-193b-3p was down-regulated in the mouse spleen and liver and in various hepatocytes (NCTC, Hepa, and HepG2 cell lines) in response to LDIR. The down-regulation of miR-193b-3p expression was caused by histone deacetylation on the miR-193b-3p promoter in the HepG2 cells irradiated with 0.01 Gy. However, the alteration of histone deacetylation and miR-193b-3p and Rad51 expression in response to LDIR was restored by pretreatment with N-acetyl-cyctein. In conclusion, we provide evidence that miRNA responses to LDIR include the modulation of cellular stress responses and repair mechanisms.

  3. Mapping of Microsatellite SW943 to Porcine Chromosome 12p11-(2/3p13) Using Primed in situ Synthesis and Somatic Cell Hybrid Panel

    LIU Bang; WANG Yong-qiang; ZHANG Qing-de; YU Mei; ZHAO Shu-hong; XIONG Tong-an; LI Kui

    2002-01-01

    The porcine microsatellite SW943 was regionally localized on 12p11-(2/3p13) by the two methods: the Primed in situ (PRINS) labelling on the pachytene bivalents of pigs using the Dig-11-dUTP as the report molecule and pig × rodent Somatic Cell Hybrid PaneI(SCHP) which contains 27 cell lines through PCR amplification. Advantages and disadvantages of the two methods for physical mapping of microsatellites were also discussed.

  4. Mild and Efficient Reagents for Oxidation of Alcohols: [MeOCH2(Ph)3P]+[CrO3X]ֿ, (X=F, Cl)

    Amir Lashgari; Shahriar Ghamami

    2015-01-01

    Two fast, mild, and reasonable oxidizing agents, Methoxymethyltriphenylphosphonium Halochromates (VI), [MeOCH2(Ph)3P]+ [CrO3X]ֿ (MMTriPPXC) are synthesized. The preparations of Methoxymethyltriphenylphosphonium Halochromates are new and efficient reagents for the oxidation of primary and secondary alcohols to their corresponding carbonyl compounds in dichloromethane at ambient temperature. These are obtained with relatively short reaction times. The oxidants/substrates ratios of 1:1 are empl...

  5. Mild and Efficient Reagents for Oxidation of Alcohols: [MeOCH2(Ph3P]+[CrO3X]ֿ, (X=F, Cl

    Amir Lashgari

    2015-01-01

    Full Text Available Two fast, mild, and reasonable oxidizing agents, Methoxymethyltriphenylphosphonium Halochromates (VI, [MeOCH2(Ph3P]+ [CrO3X]ֿ (MMTriPPXC are synthesized. The preparations of Methoxymethyltriphenylphosphonium Halochromates are new and efficient reagents for the oxidation of primary and secondary alcohols to their corresponding carbonyl compounds in dichloromethane at ambient temperature. These are obtained with relatively short reaction times. The oxidants/substrates ratios of 1:1 are employed.

  6. Spectroscopy of the Mg 1S0-3P1 intercombination transition in a luminescent cell with walls at room temperature

    Absorption at the 1S0-3P1 intercombination transition in magnesium atoms is studied experimentally. The saturated absorption resonance of thermal magnesium atoms in a compact low-pressure absorption cell with walls at room temperature was recorded for the first time by using the spatial trapping of excited magnesium atoms in the detection region and the time separation of luminescence excitation and detection. (laser spectroscopy)

  7. Measurement of the 1s2s 1S0-1s2p 3P1 intercombination interval in helium-like silicon.

    Redshaw, M; Myers, E G

    2002-01-14

    Using Doppler-tuned fast-beam laser spectroscopy the 1s2s 1S0-1s2p 3P1 intercombination interval in 28Si12+ has been measured to be 7230.5(2) cm(-1). The experiment made use of a single-frequency Nd:YAG (1.319 microm) laser and a high-finesse optical buildup cavity. The result provides a precision test of modern relativistic and QED atomic theory. PMID:11801009

  8. Correlations in Liquid Water for the TIP3P-Ewald, TIP4P-2005, TIP5P-Ewald and SWM4-NDP Models

    Huggins, David J.

    2012-01-01

    Water is one of the simplest molecules in existence, but also one of the most important in biological and engineered systems. However, understanding the structure and dynamics of liquid water remains a major scientific challenge. Molecular dynamics simulations of liquid water were performed using the water models TIP3P-Ewald, TIP4P-2005, TIP5P-Ewald, and SWM4-NDP to calculate the radial distribution functions (RDFs), the relative angular distributions, and the excess enthalpies,entropies, and...

  9. Characterization of an adapter subunit to a phosphatidylinositol (3)P 3-phosphatase: Identification of a myotubularin-related protein lacking catalytic activity

    Nandurkar, H. H.; Caldwell, K K; Whisstock, J C; Layton, M. J.; Gaudet, E. A.; Norris, F. A.; Majerus, P W; Mitchell, C. A.

    2001-01-01

    The D3-phosphoinositides act as second messengers by recruiting, and thereby activating, diverse signaling proteins. We have previously described the purification of a rat phosphatidylinositol 3-phosphate [PtdIns(3)P] 3-phosphatase, comprising a heterodimer of a 78-kDa adapter subunit in complex with a 65-kDa catalytic subunit. Here, we have cloned and characterized the cDNA encoding the human 3-phosphatase adapter subunit (3-PAP). Sequence alignment showed that 3-PAP ...

  10. Auger Spectra and Different Ionic Charges Following 3s, 3p and 3d Sub-Shells Photoionization of Kr Atoms

    Yehia A. Lotfy

    2006-01-01

    Full Text Available The decay of inner-shell vacancy in an atom through radiative and non-radiative transitions leads to final charged ions. The de-excitation decay of 3s, 3p and 3d vacancies in Kr atoms are calculated using Monte-Carlo simulation method. The vacancy cascade pathway resulted from the de-excitation decay of deep core hole in 3s subshell in Kr atoms is discussed. The generation of spectator vacancies during the vacancy cascade development gives rise to Auger satellite spectra. The last transitions of the de-excitation decay of 3s, 3p and 3d holes lead to specific charged ions. Dirac-Fock-Slater wave functions are adapted to calculate radiative and non-radiative transition probabilities. The intensity of Kr^{4+} ions are high for 3s hole state, whereas Kr^{3+} and Kr^{2+} ions have highest intensities for 3p and 3d hole states, respectively. The present results of ion charge state distributions agree well with the experimental data.

  11. Microstructure of interaction interface between Al-Si, Zn-Al alloys and Al2O3p/6061Al composite

    许志武; 闫久春; 吕世雄; 杨士勤

    2004-01-01

    Interaction behaviors between Al-Si, Zn-AI alloys and Al2O3p/6061AI composite at different heating temperatures were investigated. It is found that Al2O3p/6061Al composite can be wetted well by AlSi-1, AlSi-4 and Zn-Al alloys and an interaction layer forms between the alloy and composite during interaction. Little Al-Si alloys remain on the surface when they fully wet the composite and Si element in Al-Si alloy diffuses into composite entirely and assembles in the composite near the interface of Al-Si alloy/composite to form a Si-rich zone. The microstructure in interaction layer with Si penetration is still dense. Much more residual Zn-Al alloy exists on the surface of composite when it wets the composite, and porosities appear at the interface of Zn-Al alloy/composite. The penetration of elements Zn, Cu of Zn-Al alloy into composite leads to the generation of shrinkage cavities in the interaction layer and makes the microstructure of Al2 O3p/6061A1 composite loose.

  12. Evidence of multiple reassortment events of feline-to-human rotaviruses based on a rare human G3P[9] rotavirus isolated from a patient with acute gastroenteritis.

    Nguyen, Tinh Huu; Than, Van Thai; Thanh, Hien Dang; Kim, Wonyong

    2016-06-01

    A rare human/feline-like rotavirus G3P[9] strain, CAU14-1-262, from a 2-year-old girl with severe gastroenteritis was isolated and sequenced. The 11 gene segments of the CAU14-1-262 strain possessed a novel genotype constellation, G3-P[9]-I3-R3-C3-M3-A3-N3-T1-E3-H6, which was identified for the first time. Phylogenetic analysis of this strain identified the following genome origins: VP7, VP4, VP6, VP1-VP3, NSP1, NSP2, and NSP4 genes possessed an AU-1-like genotype 3 constellation with high sequence identity to those of the feline and human/feline-like rotaviruses; NSP5 possessed a H6 lineage, with highest sequence identity to the human/feline-like E2541 strain; and the NSP3 gene possessed a Wa-like genotype 1 constellation with high sequence identity to those of the of human rotaviruses. These results provided evidence of multiple reassortment events in G3P[9] rotavirus CAU14-1-262 and possibility of feline-to-human interspecies transmission. PMID:27260811

  13. Absolute photoionization cross section from the 6s6p {sup 1,3}P{sub 1} excited states of barium

    Kalyar, M A; Rafiq, M; Sami-ul-Haq; Baig, M A [Atomic and Molecular Physics Laboratory, Department of Physics, Quaid-i-Azam University, Islamabad 45320 (Pakistan)

    2007-06-28

    We present photoionization cross section measurements from the 6s6p {sup 1}P{sub 1} and {sup 3}P{sub 1} excited states of barium at and above the first ionization threshold. The experiments have been performed using a thermionic diode ion detector in a space charge limited mode in conjunction with a Nd:YAG laser system and the saturation technique to determine the photoionization cross sections. The absolute values of the photoionization cross section from the 6s6p {sup 1}P{sub 1} and {sup 3}P{sub 1} excited states at the first ionization threshold have been determined as 90 {+-} 14 Mb and 102 {+-} 15 Mb, respectively. The measured values of the photoionization cross section from the 6s6p {sup 1}P{sub 1} excited state are compared with available experimental and theoretical work, while the absolute photoionization cross sections from the 6s6p {sup 3}P{sub 1} excited state are reported for the first time.

  14. 3p14 De Novo Interstitial Microdeletion in a Patient with Intellectual Disability and Autistic Features with Language Impairment: A Comparison with Similar Cases

    Ana Belén de la Hoz

    2015-01-01

    Full Text Available To date, few cases of 3p proximal interstitial deletions have been reported and the phenotype and genotype correlation is not well understood. Here, we report a new case of a 3p proximal interstitial deletion. The patient is an 11-year-old female with speech and social interaction difficulties, learning disability, and slight facial dysmorphism, but no other major malformations. An 8 Mb de novo interstitial deletion at 3p14.2-p14.1, from position 60.461.316 to 68.515.453, was revealed by means of array comparative genomic hybridization and confirmed using quantitative reverse-transcription polymerase chain reaction assays. This region includes six genes: FEZF2, CADPS, SYNPR, ATXN7, PRICKLE, and MAGI1, that are known to have a role in neurodevelopment. These genes are located on the proximal side of the deletion. We compare our case with previously well-defined patients reported in the literature and databases.

  15. Absolute rate constant and O(3P yield for the O(1D+N2O reaction in the temperature range 227 K to 719 K

    S. A. Carl

    2008-10-01

    Full Text Available The absolute rate constant for the reaction that is the major source of stratospheric NOx, O(1D+N2O → products, has been determined in the temperature range 227 K to 719 K, and, in the temperature range 248 K to 600 K, the fraction of the reaction that yields O(3P. Both the rate constants and product yields were determined using a recently-developed chemiluminescence technique for monitoring O(1D that allows for higher precision determinations for both rate constants, and, particularly, O(3P yields, than do other methods. We found the rate constant, kR1, to be essentially independent of temperature between 400 K and 227 K, having a value of (1.37±0.11×10−10 cm3 s−1, and for temperatures greater than 450 K a marked decrease in rate constant was observed, with a rate constant of only (0.94±0.11×10−10 cm3 s−1 at 719 K. The rate constants determined over the 227 K–400 K range show very low scatter and are significantly greater, by 20% at room temperature and 15% at 227 K, than the current recommended values. The fraction of O(3P produced in this reaction was determined to be 0.002±0.002 at 250 K rising steadily to 0.010±0.004 at 600 K, thus the channel producing O(3P can be entirely neglected in atmospheric kinetic modeling calculations. A further result of this study is an expression of the relative quantum yields as a function of temperature for the chemiluminescence reactions (kCL1C2H + O(1D → CH(A + CO and (kCL2C2H + O(3P → CH(A + CO, both followed by CH(A → CH(X + hν, as kCL1(T/kCL2(T=(32.8T−3050/(6.29T+398.

  16. Role of chromosome 3p12–p21 tumour suppressor genes in clear cell renal cell carcinoma: analysis of VHL dependent and VHL independent pathways of tumorigenesis

    Martinez, A.; Fullwood, P; Kondo, K.; Kishida, T.; Yao, M.; Maher, E R; Latif, F

    2000-01-01

    Aims—Chromosome 3p deletions and loss of heterozygosity (LOH) for 3p markers are features of clear cell renal cell carcinoma but are rare in non-clear cell renal cell carcinoma. The VHL tumour suppressor gene, which maps to 3p25, is a major gatekeeper gene for clear cell renal cell carcinoma and is inactivated in most sporadic cases of this disease. However, it has been suggested that inactivation of other 3p tumour suppressor genes might be crucial for clear cell renal cell carcinoma tumorig...

  17. R3P-Loc: a compact multi-label predictor using ridge regression and random projection for protein subcellular localization.

    Wan, Shibiao; Mak, Man-Wai; Kung, Sun-Yuan

    2014-11-01

    Locating proteins within cellular contexts is of paramount significance in elucidating their biological functions. Computational methods based on knowledge databases (such as gene ontology annotation (GOA) database) are known to be more efficient than sequence-based methods. However, the predominant scenarios of knowledge-based methods are that (1) knowledge databases typically have enormous size and are growing exponentially, (2) knowledge databases contain redundant information, and (3) the number of extracted features from knowledge databases is much larger than the number of data samples with ground-truth labels. These properties render the extracted features liable to redundant or irrelevant information, causing the prediction systems suffer from overfitting. To address these problems, this paper proposes an efficient multi-label predictor, namely R3P-Loc, which uses two compact databases for feature extraction and applies random projection (RP) to reduce the feature dimensions of an ensemble ridge regression (RR) classifier. Two new compact databases are created from Swiss-Prot and GOA databases. These databases possess almost the same amount of information as their full-size counterparts but with much smaller size. Experimental results on two recent datasets (eukaryote and plant) suggest that R3P-Loc can reduce the dimensions by seven-folds and significantly outperforms state-of-the-art predictors. This paper also demonstrates that the compact databases reduce the memory consumption by 39 times without causing degradation in prediction accuracy. For readers׳ convenience, the R3P-Loc server is available online at url:http://bioinfo.eie.polyu.edu.hk/R3PLocServer/. PMID:24997236

  18. Exploring the dynamics of hydrogen atom release from the radical-radical reaction of O(3P) with C3H5

    The gas-phase radical-radical reaction dynamics of O(3P)+C3H5→H(2S)+C3H4O was studied at an average collision energy of 6.4 kcal/mol in a crossed beam configuration. The ground-state atomic oxygen [O(3P)] and allyl radicals (C3H5) were generated by the photolysis of NO2 and the supersonic flash pyrolysis of allyl iodide, respectively. Nascent hydrogen atom products were probed by the vacuum-ultraviolet-laser induced fluorescence spectroscopy in the Lyman-α region centered at 121.6 nm. With the aid of the CBS-QB3 level of ab initio theory, it has been found that the barrierless addition of O(3P) to C3H5 forms the energy-rich addition complexes on the lowest doublet potential energy surface, which are predicted to undergo a subsequent direct decomposition step leading to the reaction products H+C3H4O. The major counterpart C3H4O of the probed hydrogen atom is calculated to be acrolein after taking into account the factors of barrier height, reaction enthalpy, and the number of intermediates involved along the reaction pathway. The nascent H-atom Doppler profile analysis shows that the average center-of-mass translational energy of the H+C3H4O products and the fraction of the total available energy released as the translational energy were determined to be 3.83 kcal/mol and 0.054, respectively. On the basis of comparison with statistical calculations, the reaction proceeds through the formation of short-lived addition complexes rather than statistical, long-lived intermediates, and the polyatomic acrolein product is significantly internally excited at the moment of the decomposition

  19. Coordination-resolved local bond strain and 3p energy entrapment of K atomic clusters and K(1 1 0) skin

    Graphical abstract: - Highlights: • Coordination environment resolves electron binding-energy shift of K44, K46, K55 clusters. • Predict the effective coordination number of K nanoclusters when we get the atomic number N. • Atomic under coordination shortens the local bonds and entrapment. • XPS derives core level of an isolated atom and its bulk shift. - Abstract: We have examined the atomic coordination effect on the local bond strain and the 3p core-level shift of K(1 1 0) skin and nanoclusters using a combination of the bond order–length–strength correlation notion, tight-binding approach, density functional theory calculations, and photoelectron spectroscopy measurements. It turns out that: (i) the 3p core-level shifts from 15.595 ± 0.003 eV for an isolated K atom by 2.758 eV to the bulk value of 18.353 eV; (ii) the effective atomic coordination number reduces from the bulk value of 12 to 3.93 for the first layer and to 5.81 for the second layer of K(1 1 0) skin associated with the local lattice strain of 12.76%, a binding energy density 72.67%, and atomic cohesive energy −62.46% for the skin; and (iii) K cluster size reduction lowers the effective atomic coordination number and enhances further the skin electronic attribution. Results have revealed that the 3p core-level shifts of K(1 1 0) and nanoclusters originate from perturbation of the Hamiltonian by under-coordination induced charge densification and quantum entrapment

  20. Coordination-resolved local bond strain and 3p energy entrapment of K atomic clusters and K(1 1 0) skin

    Zhang, Ting; Bo, Maolin; Guo, Yongling [Key Laboratory of Low-Dimensional Materials and Application Technologies (Ministry of Education), Faculty of Materials Science and Engineering, Xiangtan University, Hunan 411105 (China); Hunan Provincial Key Laboratory of Thin Film Materials and Devices, Faculty of Materials Science and Engineering, Xiangtan University, Hunan 411105 (China); Chen, Hefeng [United Superconductive Institution, Shanghai Jiaotong University, Shanghai 200240 (China); Wang, Yan [School of Information and Electronic Engineering, Hunan University of Science and Technology, Hunan 411201 (China); Huang, Yongli, E-mail: huangyongli@xtu.edu.cn [Key Laboratory of Low-Dimensional Materials and Application Technologies (Ministry of Education), Faculty of Materials Science and Engineering, Xiangtan University, Hunan 411105 (China); Hunan Provincial Key Laboratory of Thin Film Materials and Devices, Faculty of Materials Science and Engineering, Xiangtan University, Hunan 411105 (China); Sun, Chang Q., E-mail: ecqsun@ntu.edu.sg [Key Laboratory of Low-Dimensional Materials and Application Technologies (Ministry of Education), Faculty of Materials Science and Engineering, Xiangtan University, Hunan 411105 (China); Hunan Provincial Key Laboratory of Thin Film Materials and Devices, Faculty of Materials Science and Engineering, Xiangtan University, Hunan 411105 (China); School of Information and Electronic Engineering, Hunan University of Science and Technology, Hunan 411201 (China); NOVITAS, School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798 (Singapore)

    2015-09-15

    Graphical abstract: - Highlights: • Coordination environment resolves electron binding-energy shift of K{sub 44}, K{sub 46}, K{sub 55} clusters. • Predict the effective coordination number of K nanoclusters when we get the atomic number N. • Atomic under coordination shortens the local bonds and entrapment. • XPS derives core level of an isolated atom and its bulk shift. - Abstract: We have examined the atomic coordination effect on the local bond strain and the 3p core-level shift of K(1 1 0) skin and nanoclusters using a combination of the bond order–length–strength correlation notion, tight-binding approach, density functional theory calculations, and photoelectron spectroscopy measurements. It turns out that: (i) the 3p core-level shifts from 15.595 ± 0.003 eV for an isolated K atom by 2.758 eV to the bulk value of 18.353 eV; (ii) the effective atomic coordination number reduces from the bulk value of 12 to 3.93 for the first layer and to 5.81 for the second layer of K(1 1 0) skin associated with the local lattice strain of 12.76%, a binding energy density 72.67%, and atomic cohesive energy −62.46% for the skin; and (iii) K cluster size reduction lowers the effective atomic coordination number and enhances further the skin electronic attribution. Results have revealed that the 3p core-level shifts of K(1 1 0) and nanoclusters originate from perturbation of the Hamiltonian by under-coordination induced charge densification and quantum entrapment.

  1. Summary Report for National Aeronautics Space Administration (NASA) and Centro Para Prevencao da Poluicao (C3P) 2011 International Workshop on Environment and Alternative Energy

    Greene, Brian

    2011-01-01

    The C3P &. NASA International Workshop on Environment and Alternative Energy was held on November 15-18, 2011 at the European Space Agency (ESA)'s Research and Technology Centre (ESTEC) in Noordwijk, The Netherlands. The theme of the workshop was "Global Collaboration in Environmental and Alternative Energy Strategies". The workshop was held at ESTEC's conference center. More than 110 individuals from eleven countries attended the workshop. For the first time since the inception of NASA-C3P workshops, a full day was dedicated to a student session. Fifteen students from around the globe gave oral presentations along with poster displays relating to the latest technologies in environmental and alternative energy strategies. Judges from NASA, C3P and ESA awarded plaques to the top three students. In addition to the students, thirty eight U.S. and international subject matter experts presented on the following general environmental-related topics: (1) Hazardous materials management and substitution in support of space operations (2) Emerging renewable and alternative energy technologies (3) Sustainable development and redevelopment (4) Remediation technologies and strategies The workshop also included a panel discussion on the topic of the challenges of operating installations across borders. Throughout the workshop, attendees heard about the scope of environmental and energy challenges that industry and governments face. They heard about technologies for increasing energy efficiency and increasing use of renewable energy. They learned about ways companies and government agencies are using materials, processes, goods and services in a manner more respectful with the environment and in compliance with health and safety rules. The concept of partnerships and their inherent benefits was evidenced throughout the workshop. Partnering is a key aspect of sustainability because sustainable development is complicated. Through formal presentations and side discussions, attendees

  2. Accurate determination of the fine-structure intervals in the 3P ground states of C-13 and C-12 by far-infrared laser magnetic resonance

    Cooksy, A. L.; Saykally, R. J.; Brown, J. M.; Evenson, K. M.

    1986-01-01

    Accurate values are presented for the fine-structure intervals in the 3P ground state of neutral atomic C-12 and C-13 as obtained from laser magnetic resonance spectroscopy. The rigorous analysis of C-13 hyperfine structure, the measurement of resonant fields for C-12 transitions at several additional far-infrared laser frequencies, and the increased precision of the C-12 measurements, permit significant improvement in the evaluation of these energies relative to earlier work. These results will expedite the direct and precise measurement of these transitions in interstellar sources and should assist in the determination of the interstellar C-12/C-13 abundance ratio.

  3. Untersuchungen zur Funktion der MicroRNAs miR-199a/b-3p und miR-223 im kardiovaskulären System

    Leierseder, Simon Johannes

    2016-01-01

    In MicroRNA-Expressionsanalysen an isolierten Herzzellen wurden miR-199a/b 3p und miR-223 als hochgradig in kardialen Fibroblasten gegenüber Kardiomyozyten angereicherte MicroRNAs identifiziert und deshalb für weiterführende Studien ausgewählt. Die Ergebnisse von Untersuchungen an kultivierten Fibroblasten und in Mausmodellen für kardialen Stress legen nahe, dass beide MicroRNAs wichtige Rollen im Herz übernehmen. Darüber hinaus zeigte die Untersuchung von Blutplättchen und Megakaryozyten, da...

  4. Formation and decay of (3P(J))O atoms in the laser flash photolysis of chlorine dioxide (OClO) at 308 nm

    Colussi, A. J.

    1990-01-01

    The quantum yields of O(3P(J)) and Cl(2P(3/2)) atoms released in the laser flash photolysis of OClO at 308 and 298 K were determined and the kinetics of the subsequent oxygen atom decay was investigated using time-resolved atomic resonance fluorescence measurements. The results are consistent with the formation of sym-ClO3 having a Delta Hf(ClO3) (formed in the reaction O + OClO + Ar) of 55.6 +/-4 kcal/mol.

  5. The influence of SiO2 Addition on 2MgO-Al2O3-3.3P2O5 Glass

    Larsen, P.H.; Poulsen, F.W.; Berg, Rolf W.

    1999-01-01

    2MgO-Al2O3-3.3P2O5 glasses with increasing amounts of SiO2 are considered for sealing applications in Solid Oxide Fuel Cells (SOFC). The change in chemical durability under SOFC anode conditions and the linear thermal expansion is measured as functions of the SiO2 concentration. Raman spectroscopy...... analysis of the glasses reveals no sign of important changes in the glass structure upon SiO2 addition. Some increase in glass durability with SiO2 concentration is reported and its cause is discussed....

  6. A high precision beam-foil meanlife measurement of the 1s3p1P level in HeI

    A beam-foil measurement of the meanlife of the 1s3p1P level in HeI has been made and yields the value 1.7225 +- 0.0046 ns. The measurement was made with standard beam-foil techniques and equipment, but special attention was devoted to minimizing sources of uncertainty. The precision far exceeds that of previous beam-foil meanlife measurements and demonstrates that the beam-foil technique is capable of high precision and is competitive with and more flexible than methods such as resonant laser excitation. (orig.)

  7. Aberrantly expressed miR-582-3p maintains lung cancer stem cell-like traits by activating Wnt/β-catenin signalling

    Fang, Lishan; Cai, Junchao; Chen, Baixue; Wu, Shanshan; Li, Rong; Xu, Xiaonan; Yang, Yi; Guan, Hongyu; Zhu, Xun; Zhang, Le; Yuan, Jie; Wu, Jueheng; Li, Mengfeng

    2015-01-01

    Cancer stem cells (CSCs) are involved in tumorigenesis, tumour recurrence and therapy resistance and Wnt signalling is essential for the development of the biological traits of CSCs. In non-small cell lung carcinoma (NSCLC), unlike in colon cancer, mutations in β-catenin and APC genes are uncommon; thus, the mechanism underlying the constitutive activation of Wnt signalling in NSCLC remains unclear. Here we report that miR-582-3p expression correlates with the overall- and recurrence-free-sur...

  8. Asperger syndrome in a boy with a balanced de novo translocation: t(17;19)(p13.3;p11)

    NONE

    1995-04-10

    The cause of Asperger syndrome is unknown. It is classified as a form of childhood autism. Familial aggregation in infantile autism has been reported. Asperger syndrome has also been considered as being genetically transmitted and certain of its characteristics have tended to occur in other relatives, especially in the fathers. We describe a 10-year-old boy with a clinical diagnosis of Asperger syndrome and a balanced de novo translocation (t(17;19)9p13.3;p11). His parents are not consanguineous and are healthy, as are his three brothers. 5 refs.

  9. Relativistic MCDF oscillator strengths for 6s21S0-6s6p 1P1,3P1 transitions in lanthanide ions

    The multiconfiguration Dirac-Fock calculation of the transition energies and oscillator strengths for the 6s21S0-6s6p 1P1,3P1 transitions in rare earth ionized systems (from La+1 through Nd+4) were carried out. At least part of electron correlation was taken into account. The intravalence correlation was represented through limited relativistic configuration mixing while the valence-core correlation was approximated by a core polarization model. For the allowed transition, we compared our results with other theoretical data, whereas for the forbidden transition only our results are available

  10. Cross sections for the excitation of 3s,3p,3d,4p, and 4s manifolds in e-Ne collisions

    Theoretical differential and integral cross sections are reported for inelastic scattering of electrons by neon atoms for incident electron energies in the range of 20-100 eV. Transitions from the ground state to forty states associated with the 3s, 3p, 3d, 4s, and 4p manifolds were considered. The methods employed were the distorted wave approximation and first-order many-body theory, where the distorting potential includes the static-exchange potential. A comparison of our results with experimental data and other theoretical results are shown and discussed. (author)

  11. Influence of the 6^1S_0-6^3P_1 Resonance on Continuous Lyman-alpha Generation in Mercury

    Kolbe, Daniel; Walz, Jochen

    2012-01-01

    Continuous coherent radiation in the vacuum-ultraviolet at 122 nm (Lyman-alpha) can be generated using sum-frequency mixing of three fundamental laser beams in mercury vapour. One of the fundamental beams is at 254 nm wavelength, which is close to the 6^1S_0-6^3P_1 resonance in mercury. Experiments have been performed to investigate the effect of this one-photon resonance on phasematching, absorption and the nonlinear yield. The efficiency of continuous Lyman-alpha generation has been improved by a factor of 4.5.

  12. Calculation of Electron-Impact 2s22p4 3P → 2p33s3SO Transition in Atomic Oxygen

    WANG Yang; LI Yan-Hua; ZHOU Ya-Jun

    2005-01-01

    The inelastic scattering of electron from oxygen for the 2s22p4 3P → 2p33s 3SO transition is studied using the momentum-space coupled channel optical method at 15, 17.5, 20, 22.5, 25, 27.5, 30, 50, and 100eV. Direct ionization cross sections, i.e. differential and integral cross sections, are reported. Important continuum states are included in the coupled channel calculation via a complex equivalent-local optical potential. The present results are compared with the available experimental data and other theoretical calculation results.

  13. Inhibition of lung cancer cell growth and induction of apoptosis after reexpression of 3p21.3 candidate tumor suppressor gene SEMA3B

    Tomizawa, Yoshio; Sekido, Yoshitaka; Kondo, Masashi; Gao, Boning; Yokota, Jun; Roche, Joëlle; Drabkin, Harry; Lerman, Michael I; Gazdar, Adi F.; Minna, John D.

    2001-01-01

    Semaphorins SEMA3B and its homologue SEMA3F are 3p21.3 candidate tumor suppressor genes (TSGs), the expression of which is frequently lost in lung cancers. To test the TSG candidacy of SEMA3B and SEMA3F, we transfected them into lung cancer NCI-H1299 cells, which do not express either gene. Colony formation of H1299 cells was reduced 90% after transfection with wild-type SEMA3B compared with the control vector. By contrast, only 30–40% reduction in colony formation was seen after the transfec...

  14. Accurate determination of the fine-structure intervals in the 3P ground states of C-13 and C-12 by far-infrared laser magnetic resonance

    Accurate values are presented for the fine-structure intervals in the 3P ground state of neutral atomic C-12 and C-13 as obtained from laser magnetic resonance spectroscopy. The rigorous analysis of C-13 hyperfine structure, the measurement of resonant fields for C-12 transitions at several additional far-infrared laser frequencies, and the increased precision of the C-12 measurements, permit significant improvement in the evaluation of these energies relative to earlier work. These results will expedite the direct and precise measurement of these transitions in interstellar sources and should assist in the determination of the interstellar C-12/C-13 abundance ratio. 16 references

  15. Na2O.B2O3-Al2O3-P2O5 system as a medium for vitrification of nuclear power plant wastes

    Laboratory experiments have shown that in the studied system 3Na2O.B2O3-Al2O3-P2O5 in the area of the delineated simplex triangle it is possible to obtain glasses which are very easily melted at temperatures of approximately 1100 degC. These glasses have only a slight tendency to crystallization and a high dilatability, especially in the region of transformation. Also studied was the hydrolytical and chemical resistance of different glass samples. The pilot plant experiment verified the possibility of the fixation of radioactive wastes in material based on sodium borophosphates. (J.B.)

  16. Interference effect involving doubly excited states [1s2p](3,1P)3p2 (J=1) in 1s photoionization of neon

    Within the framework of multiconfiguration Dirac-Fock (MCDF) method, the interference between photoionization and photoexcitation autoionization involving the doubly excited states [1s2p](3,1P)3p2 (J=1) of neon have been studied theoretically. The present results indicate the fine structure of the interference spectra in experiment and show the dominant contribution to the total profile resulting from some individual resonances. Reasonable agreement is found between the present calculations and other available theoretical and experimental results. In addition, some interesting trends of variation of Fano parameters q and ρ2 have also been pointed out. (author)

  17. Note on the 2s21So-2s2p 3P1 intercombination line of B II and C III

    Revised transition rates are reported for the 2s21S0-2s2p 3P1 intercombination line of 10.27 ± 0.20 s-1 for B II and 103.0 ± 0.4 s-1 for C III. These MCHF values are based on calculations that correct an error in the spin-other-orbit interaction of previous results and apply the most recent techniques for normalizing Breit-Pauli results for intercombination lines and estimating uncertainties. (orig.)

  18. Radiative lifetime of the 3s3p exp 3(exp 5 S sub 2 exp 0) metastable level of P(+)

    Calamai, Anthony G.; Han, Xiaofeng; Parkinson, William H.

    1992-01-01

    The present experimental and theoretical results for the radiative lifetime of the 3s3p exp 3(exp 5 S sub 2 exp 0) metastable level of P(+) encompass an experimental determination of the (exp 5 S sub 2 exp 0) lifetime which represents the first measured lifetime of a low charge-state ion in the Si I sequence. This constitutes a fundamental test of the theoretical methods used to determine transition possibilities for intercombination lines involving this level, and suggests that theoretical techniques used to determine such transition probabilities in low-Z species of the Si I isoelectronic sequence should be reevaluated.

  19. Improved measurement of the 1s2s 1S0-1s2p 3P1 interval in heliumlike silicon.

    DeVore, Thomas R; Crosby, David N; Myers, Edmund G

    2008-06-20

    Using colinear fast-beam laser spectroscopy with copropagating and counter-propagating beams we have measured the 1s2s 1S0-1s2p 3P1 intercombination interval in 28Si12+ with the result 7230.585(6) cm{-1}. The experiment made use of a dual-wavelength, high-finesse, power build-up cavity excited by single-frequency lasers at 1319 and 1450 nm. The result will provide a precision test of ab initio relativistic many-body atomic theory at moderate Z. PMID:18643579

  20. Transition probabilities for the 1s21S0-1s2p 3P1 intercombination line in He-like carbon and nitrogen

    Lifetime measurements are presented for 1s2p 3Psub(1,2) levels for He-like C V and N VI. From the measured lifetimes, transition probabilities are deduced for both allowed and spin-forbidden transitions. The values for the 1s21S0 - 1s2p 3P1 transition rates, 0.29 . 108 and 1.38 . 108 s-1 for C V and N VI respectively, are found to be in good agreement with theory. (orig.)

  1. The 3P Learning Model

    Chatti, Mohamed Amine; Jarke, Matthias; Specht, Marcus

    2010-01-01

    Recognizing the failures of traditional Technology Enhanced Learning (TEL) initiatives to achieve performance improvement, we need to rethink how we design new TEL models that can respond to the learning requirements of the 21st century and mirror the characteristics of knowledge and learning which are fundamentally personal, social, distributed,…

  2. Quasiclassical trajectory studies of 18O(3P) + NO2 isotope exchange and reaction to O2 + NO on D0 and D1 potentials

    Fu, Bina; Zhang, Dong H.; Bowman, Joel M.

    2013-07-01

    We report quasiclassical trajectory calculations for the bimolecular reaction 18O(3P) + NO2 on the recent potential energy surfaces of the ground (D0) and first excited (D1) states of NO3 [B. Fu, J. M. Bowman, H. Xiao, S. Maeda, and K. Morokuma, J. Chem. Theory. Comput. 9, 893 (2013)], 10.1021/ct3009792. The branching ratio of isotope exchange versus O2 + NO formation, as well as the product angular distributions and energy and rovibrational state distributions are presented. The calculations are done at the collision energy of relevance to recent crossed beam experiments [K. A. Mar, A. L. Van Wyngarden, C.-W. Liang, Y. T. Lee, J. J. Lin, and K. A. Boering, J. Chem. Phys. 137, 044302 (2012)], 10.1063/1.4736567. Very good agreement is achieved between the current calculations and these experiments for the branching ratio and final translational energy and angular distributions of isotope exchange products 16O(3P) + NO2 and O2 + NO formation products. The reactant 18O atom results in 18O16O but not N18O for the O2 + NO formation product channel, consistent with the experiment. In addition, the detailed vibrational and rotational state information of diatomic molecules calculated currently for the 34O2 + NO formation channel on D0 and D1 states are in qualitative agreement with the previous experimental and theoretical results of the photodissociation of NO3 and are consistent with older thermal bimolecular kinetics measurements.

  3. The effect of Sm, Eu and Yb on the thermoelectric properties of Th/sub 3/P/sub 4/ - type lanthanum sulfide

    The Th/sub 3/P/sub 4/-type lanthanum sulfides are n-type semiconductors and a continuous series of solid solutions is found between the compositions of La/sub 3/S/sub 4/ and La/sub 2/S/sub 3/. These materials are attractive for use in high temperature thermoelectric applications because of their high melting points, low thermal conductivities and their inherent ability to vary the electron concentration from a maximum of 6.02 X 10/sup 21/ cm/sup -3/ for La/sub 3/S/sub 4/ to zero for La/sub 2/S/sub 3/ (i.e., self-doping). In this study, La/sub 3/S/sub 4/ compounds in which trivalent La has been partially substituted by divalent Sm, Eu or Yb (La/sub 3-x/M/sub x/S/sub 4/, x = 0.1 to 0.9) have been prepared by the pressure assisted reaction sintering method and were found to have the Th/sub 3/P/sub 4/ structure. The thermoelectric properties (Seebeck coefficient and electrical resistivity) were measured as a function of temperature and the optimum composition was found for x between 0.2 and 0.3 in which the electrical power factor was a maximum at 10000C

  4. Intramultiplet mixing in collisions of calcium 4s4p 3P/sub J//sup o/ with helium: Measurement of state-to-state cross sections

    An optical-pumping state-selection technique for the study of collisions of 3P atoms in individual spin-orbit states is described. This technique is used to determine state-to-state intramultiplet-mixing cross sections for collisions of Ca 3P/sup o/ with helium in a beam--static-gas arrangement. The derived cross sections are as follows: for J = 2→J' = 1, 31.9 +- 4.2 A2; for J = 2→J' = 0, 5.5 +- 1.6 A2; and for J = 1→J' = 0, 2.0 +- 5.0 A2. These quantities represent averages over the translational-energy distribution (average energy 0.0556 eV). In the following paper Alexander, Orlikowski, and Straub present quantum close-coupling cross sections as a function of translational energy, using the pseudopotential Ca-He interaction curves of Malvern [J. Phys. B 11, 831 (1978)]. The experimental 2→1 and 2→0 cross sections are found to be 3 to 4 times larger than the calculated theoretical values. In contrast to the theoretical prediction for the ratio of the smaller cross sections, the 2→0 cross section is found to be larger than the 1→0 cross section

  5. A Gas-Phase Investigation of Oxygen-Hydrogen Exchange Reaction of O(3P) + C2H5 → H(2S) + C2H4O

    The gas-phase radical-radical reaction O(3P) + C2H5 (ethyl) → H(2S) + CH3CHO (acetaldehyde) was investigated by applying a combination of vacuum-ultraviolet laser-induced fluorescence spectroscopy in a crossed beam configuration and ab initio calculations. The two radical reactants O(3P) and C2H5 were respectively produced by photolysis of NO2 and supersonic flash pyrolysis of the synthesized precursor azoethane. Doppler profile analysis of the nascent H-atom products in the Lyman-α region revealed that the average translational energy of the products and the average fraction of the total available energy released as translational energy were 5.01 ± 0.72 kcal mol-1 and 6.1%, respectively. The empirical data combined with CBS-QB3 level ab initio theory and statistical calculations demonstrated that the title exchange reaction is a major channel and proceeds via an addition-elimination mechanism through the formation of a short-lived, dynamical addition complex on the doublet potential energy surface. On the basis of systematic comparison with several exchange reactions of hydrocarbon radicals, the observed small kinetic energy release can be explained in terms of the loose transition state with a product-like geometry and a small reverse activation barrier along the reaction coordinate

  6. A novel syndrome of paediatric cataract, dysmorphism, ectodermal features, and developmental delay in Australian Aboriginal family maps to 1p35.3-p36.32

    Gecz Jozef

    2010-11-01

    Full Text Available Abstract Background A novel phenotype consisting of cataract, mental retardation, erythematous skin rash and facial dysmorphism was recently described in an extended pedigree of Australian Aboriginal descent. Large scale chromosomal re-arrangements had previously been ruled out. We have conducted a genome-wide scan to map the linkage region in this family. Methods Genome-wide linkage analysis using Single Nucleotide Polymorphism (SNP markers on the Affymetrix 10K SNP array was conducted and analysed using MERLIN. Three positional candidate genes (ZBTB17, EPHA2 and EPHB2 were sequenced to screen for segregating mutations. Results Under a fully penetrant, dominant model, the locus for this unique phenotype was mapped to chromosome 1p35.3-p36.32 with a maximum LOD score of 2.41. The critical region spans 48.7 cM between markers rs966321 and rs1441834 and encompasses 527 transcripts from 364 annotated genes. No coding mutations were identified in three positional candidate genes EPHA2, EPHB2 or ZBTB17. The region overlaps with a previously reported region for Volkmann cataract and the phenotype has similarity to that reported for 1p36 monosomy. Conclusions The gene for this syndrome is located in a 25.6 Mb region on 1p35.3-p36.32. The known cataract gene in this region (EPHA2 does not harbour mutations in this family, suggesting that at least one additional gene for cataract is present in this region.

  7. Doppler-free spectroscopy of the 1S0-3P0 optical clock transition in laser-cooled fermionic isotopes of neutral mercury

    Petersen, M; Dawkins, S T; Magalhães, D V; Mandache, C; Lecoq, Y; Clairon, A; Bize, S

    2008-01-01

    We have performed for the first time direct laser spectroscopy of the 1S0-3P0 optical clock transition at 265.6 nm in fermionic isotopes of neutral mercury laser-cooled in a magneto-optical trap. Spectroscopy is performed by measuring the depletion of the magneto-optical trap induced by the excitation of the long-lived 3P0 state by a probe at 265.6 nm. Measurements resolve the Doppler-free recoil doublet allowing for a determination of the transition frequency to an uncer- tainty well below the Doppler-broadened linewidth. We have performed absolute measurement of the frequency with respect to an ultra-stable reference monitored by LNE-SYRTE fountain pri- mary frequency standards using a femtosecond laser frequency comb. The measured frequency is 1128575290808 +/- 5.6 kHz in 199Hg and 1128569561140 +/- 5.3 kHz in 201Hg, more than 4 orders of magnitude better than previous indirect determinations. Owing to a low sensitivity to blackbody radiation, mercury is a promising candidate for reaching the ultimate perf...

  8. Molecular cloning of t(2;7)(p24.3;p14.2), a novel chromosomal translocation in myelodysplastic syndrome-derived acute myeloid leukemia.

    Fujita, Kazuhiro; Sanada, Masashi; Harada, Hiroshi; Mori, Hiraku; Niikura, Haruo; Omine, Mitsuhiro; Inazawa, Johji; Imoto, Issei

    2009-06-01

    In this study, we report the molecular structure of the breakpoint region in a new chromosomal translocation, t(2;7)(p24.3;p14.2), in a case of acute myeloid leukemia transformed from myelodysplastic syndrome (MDS). An extensive fluorescence in situ hybridization (FISH) analysis showed that NAG (2p24.3) and ELMO1 (7p14.2) were involved at the breakpoints of t(2;7)(p24.3;p14.2). Furthermore, we detected a novel chimeric transcript consisting of NAG and ELMO1. Interestingly, this transcript encoded a truncated molecular form of 3'ELMO1 as the result of a frameshift caused by the chromosomal translocation. Although this study does not provide direct evidence that a defect in NAG-ELMO1 plays a role in the pathogenesis or the leukemic change in MDS, it does suggest that defects in NAG-ELMO1 potentially contributed to the leukemic progression in this case. PMID:19407829

  9. Effects of electron correlation, electron-phonon coupling, and spin-orbit coupling on the isovalent Pd-substituted superconductor SrPt3P

    Hu, Kangkang; Gao, Bo; Ji, Qiucheng; Ma, Yonghui; Li, Wei; Xu, Xuguang; Zhang, Hui; Mu, Gang; Huang, Fuqiang; Cai, Chuanbing; Xie, Xiaoming; Jiang, Mianheng

    2016-06-01

    We present a systematical study on the roles of interactions among electron correlation, electron-phonon coupling, and spin-orbit coupling in the isovalent Pd-substituted superconductor SrPt3P . By using the solid state reaction method, the Pd element with the 4 d orbital was successfully substituted in the strong spin-orbit coupling superconductors Sr (Pt1-xPdx) 3P . As increasing the isovalent Pd concentrations without introducing any extra electron/hole carriers, the superconducting transition temperature Tc decreases monotonously. In addition, combining the data of resistivity and specific heat, as well as electronic band structure calculations, we found that the electron correlation is enhanced while the electron-phonon coupling and the spin-orbit coupling are suppressed by Pd substitution. Our results may provide significant insights in the natures of the interplay among the electron correlation, electron-phonon coupling, and spin-orbit coupling in superconductivity, and may also pave a route for understanding the mechanism of superconductivity in heavily 5 d -based superconductors.

  10. Green Polymer Chemistry: Investigating the Mechanism of Radical Ring-Opening Redox Polymerization (R3P of 3,6-Dioxa-1,8-octanedithiol (DODT

    Emily Q. Rosenthal-Kim

    2015-04-01

    Full Text Available The mechanism of the new Radical Ring-opening Redox Polymerization (R3P of 3,6-dioxa-1,8-octanedithiol (DODT by triethylamine (TEA and dilute H2O2 was investigated. Scouting studies showed that the formation of high molecular weight polymers required a 1:2 molar ratio of DODT to TEA and of DODT to H2O2. Further investigation into the chemical composition of the organic and aqueous phases by 1H-NMR spectroscopy and mass spectrometry demonstrated that DODT is ionized by two TEA molecules (one for each thiol group and thus transferred into the aqueous phase. The organic phase was found to have cyclic disulfide dimers, trimers and tetramers. Dissolving DODT and TEA in water before the addition of H2O2 yielded a polymer with Mn = 55,000 g/mol, in comparison with Mn = 92,000 g/mol when aqueous H2O2 was added to a DODT/TEA mixture. After polymer removal, MALDI-ToF MS analysis of the residual reaction mixtures showed only cyclic oligomers remaining. Below the LCST for TEA in water, 18.7 °C, the system yielded a stable emulsion, and only cyclic oligomers were found. Below DODT/TEA and H2O2 1:2 molar ratio mostly linear oligomers were formed, with <20% cyclic oligomers. The findings support the proposed mechanism of R3P.

  11. Cross sections for electron capture and excitation in proton collisions with the metastable Be(2s2p3P) atom

    Previous studies have shown that it appears that the metastable Be(1s22s2p 3P) atoms can constitute a significant fraction of the atomic beryllium in the ITER plasma edge. The two-center atomic orbital close-coupling method is employed to study electron capture and excitation processes in proton collisions with the metastable Be(1s22s2p 3P) atom. The interaction of the active electron with the Be+ ion core is represented by a model potential reproducing accurately (to within 3.5%) the energies of triplet excited states Be(1s22snl 3L) (at least up to n = 7). The excitation and state-selective electron capture cross sections up to the n = 5 shells of both centers are calculated in the energy range 1-200 keV/u using an expansion basis involving all the states with n ≤ 7 on H and all triplet states with n ≤ 7 on Be, augmented with a number of pseudo-states. In the energy region below ∼ 10 keV/u, the cross sections of both processes exhibit oscillatory structures, resulting from the multi-state coupling accounted for in the dynamical model. It has been found that the magnitude of excitation cross sections above ∼ 10 keV/u is dominated by the multipole interactions, while below this energy the excitation process proceeds through intermediate electron capture states

  12. 甲状腺肿瘤3号染色体短臂杂合性缺失的研究%Loss of heterozygosity on chromosome 3p in thyroid tumors

    胡孟钧; 徐航娣; 周韧; 郦秀芳; 张海勇

    2008-01-01

    目的 探讨甲状腺肿瘤中3号染色体短臂(3p)杂合性缺失(LOH)状态及其临床意义.方法 收集74例甲状腺肿瘤标本,包括20例甲状腺腺瘤(FA)、24例滤泡性甲状腺癌(FTC)和30例乳头状甲状腺癌(Prc).通过PCR扩增和银染分析其3p上11个微卫星位点的杂合性缺失状态.结果 FFC的LOH频率达到71%(17/24),PTC中30%(9/30),FA中10%(2/20).FFC的3p LOH频率显著高于FA和PTC(P<0.01).FTC中存在两个最小共同缺失区,分别位于3p26-pter和3p14.2-3p22.PTC上存在一个最小共同缺失区,位于3p 25.2-26.1.结论 FTC的3p LOH频率显著高于FA和PTC.3p的3个最小缺失区上可能存在着与FTC和PTC发生发展相关的肿瘤抑制基因.%Objective To study the loss of heterozygosity(LOH)on chromosome 3p in thyroid tumors. Methods LOH at 11 microsatellite loci Wag analyzed in 74 cases of thyroid tumors(including 20 follicular adenomas, 24 follicular thyroid carcinomas and 30 papillary thyroid carcinomas)by polymerase chain reaction and silver stain. Results LOH on chromosome 3p Wag detected in 71% of follicular thyroid carcinoma(17/24), 30%of the papillary thyroid carcinoma(9/30) and 10%of the follicular adenoma (2/20)case. Two minimal common deleted regions(CDR)(3p26-pter and 3p14. 2-3p22)involving significant sites of LOH has identified in follicular thyroid carcinoma. There Was also one CDR(3p25. 2-26. 1)in papilhry thyroid careinoma. Conclusions LOH is more frequently identified in follicular thyroid careinoma than in papillary thyroid carcinoma and follicular adenoma. The 3 CDR on chromosome 3p may harbor tumor suppressor genes involved in the pathogenesis of follicular thyroid carcinoma and papillary thyroid carcinoma.

  13. MiR-223-3p as a Novel MicroRNA Regulator of Expression of Voltage-Gated K+ Channel Kv4.2 in Acute Myocardial Infarction

    Xue Liu

    2016-06-01

    Full Text Available Background/Aims: Acute myocardial infarction (AMI is a devastating cardiovascular disease with a high rate of morbidity and mortality, partly due to enhanced arrhythmogenicity. MicroRNAs (miRNAs have been shown to participate in the regulation of cardiac ion channels and the associated arrhythmias. The purpose of this study was to test our hypothesis that miR-223-3p contributes to the electrical disorders in AMI via modulating KCND2, the gene encoding voltage-gated channel Kv4.2 that carries transient outward K+ current Ito. Methods: AMI model was established in male Sprague-Dawley (SD rats by left anterior descending artery (LAD ligation. Evans blue and TTC staining was used to measure infarct area. Ito was recorded in isolated ventricular cardiomyocytes or cultured neonatal rat ventricular cells (NRVCs by whole-cell patch-clamp techniques. Western blot analysis was employed to detect the protein level of Kv4.2 and real-time RT-PCR to determine the transcript level of miR-223-3p. Luciferase assay was used to examine the interaction between miR-223-3p and KCND2 in cultured NRVCs. Results: Expression of miR-223-3p was remarkably upregulated in AMI relative to sham control rats. On the contrary, the protein level of Kv4.2 and Ito density were significantly decreased in AMI. Consistently, transfection of miR-223-3p mimic markedly reduced Kv4.2 protein level and Ito current in cultured NRVCs. Co-transfection of AMO-223-3p (an antisense inhibitor of miR-223-3p reversed the repressive effect of miR-223-3p. Luciferase assay showed that miR-223-3p, but not the negative control, substantially suppressed the luciferase activity, confirming the direct binding of miR-223-3p to the seed site within the KCND2 sequence. Finally, direct intramuscular injection of AMO-223-3p into the ischemic myocardium to knockdown endogenous miR-223-3p decreased the propensity of ischemic arrhythmias. Conclusions: Upregulation of miR-223-3p in AMI repressed the expression of

  14. BOT3P5.2, 3D Mesh Generator and Graphical Display of Geometry for Radiation Transport Codes, Display of Results

    1 - Description of program or function: BOT3P was originally conceived as a set of standard FORTRAN 77 language programs in order to give the users of the DORT and TORT deterministic transport codes some useful diagnostic tools to prepare and check their input data files. Later versions extended the possibility to produce the geometrical, material distribution and fixed neutron source data to other deterministic transport codes such as TWODANT/THREEDANT of the DANTSYS system, PARTISN and, potentially, to any transport code through BOT3P binary output files that can be easily interfaced (see, for example, the Russian two-dimensional (2D) and three-dimensional (3D) discrete ordinates neutron, photon and charged particle transport codes KASKAD-S-2.5 and KATRIN-2.0). As from Version 5.1 BOT3P contained important additions specifically addressed to radiation transport analysis for medical applications. BOT3P-5.2 contains new graphics capabilities. Some of them enable users to select space sub-domains of the total mesh grid in order to improve the zoom simulation of the geometry, both in 2D cuts and in 3D. Moreover the new BOT3P module (PDTM) may improve the interface of BOT3P geometrical models to transport analysis codes. The following programs are included in the BOT3P software package: GGDM, DDM, GGTM, DTM2, DTM3, RVARSCL, COMPARE, MKSRC, CATSM, DTET, and PDTM. The main features of these different programs are described. 2 - Methods: GGDM and GGTM work similarly from the logical point of view. Since the 3D case is more general, the following description refers to GGTM. All the co-ordinate values that characterise the geometrical scheme at the basis of the 3D transport code geometrical and material model are read, sorted and all stored if different from the neighbouring ones more than an input tolerance established by the user. These co-ordinates are always present in the fine-mesh boundary arrays independently of the mesh grid refinement options, because they

  15. 类硅铑离子的3s23p23P1,2-3s3p35S2的跃迁谱线%The Intercombination Lines of 3s23p23P1,2-3s3p35S2 in Si-like Ions, Rhodium

    2002-01-01

    用束箔法研究了类硅铑离子的3s23p23P1,2-3s3p35S2的禁戒跃迁谱线.谱线识别从已知基态精细结构的分裂、基于分支比的强度比、相似的衰减特性、离子束能量下的谱线预期值方面着手.识别后,通过对已知谱线的波长的等电子系列曲线插值或外推来获得用于较刻的谱线的波长,然后较刻出3s23p23P1,2-3s3p35S2这两条谱线.

  16. Ba3(P1−MnO4)2 : Blue/green inorganic materials based on tetrahedral Mn(V)

    Sourav Laha; Rohit Sharma; S V Bhat; M L P Reddy; J Gopalakrishnan; S Natarajan

    2011-10-01

    We describe a blue/green inorganic material, Ba3(P1−MnO4)2 (I) based on tetrahedral MnO$^{3-}_{4}$ :32 chromophore. The solid solutions (I) which are sky-blue and turquoise-blue for ≤ 0.25 and dark green for ≥ 0.50, are readily synthesized in air from commonly available starting materials, stabilizing the MnO$^{3-}_{4}$ chromophore in an isostructural phosphate host. We suggest that the covalency/ionicity of P–O/Mn–O bonds in the solid solutions tunes the crystal field strength around Mn(V) such that a blue colour results for materials with small values of . The material could serve as a nontoxic blue/green inorganic pigment.

  17. Genome wide high density SNP-based linkage analysis of childhood absence epilepsy identifies a susceptibility locus on chromosome 3p23-p14

    Chioza, Barry A; Aicardi, Jean; Aschauer, Harald;

    2009-01-01

    and the genes involved are yet to be fully established. A genome wide single nucleotide polymorphism (SNP)-based high density linkage scan was carried out using 41 nuclear pedigrees with at least two affected members. Multipoint parametric and non-parametric linkage analyses were performed using...... MERLIN 1.1.1 and a susceptibility locus was identified on chromosome 3p23-p14 (Z(mean)=3.9, p<0.0001; HLOD=3.3, alpha=0.7). The linked region harbours the functional candidate genes TRAK1 and CACNA2D2. Fine-mapping using a tagSNP approach demonstrated disease association with variants in TRAK1....

  18. Deletions in chromosome 6p22.3-p24.3, including ATXN1, are associated with developmental delay and autism spectrum disorders

    Celestino-Soper Patrícia BS

    2012-04-01

    Full Text Available Abstract Interstitial deletions of the short arm of chromosome 6 are rare and have been associated with developmental delay, hypotonia, congenital anomalies, and dysmorphic features. We used array comparative genomic hybridization in a South Carolina Autism Project (SCAP cohort of 97 subjects with autism spectrum disorders (ASDs and identified an ~ 5.4 Mb deletion on chromosome 6p22.3-p23 in a 15-year-old patient with intellectual disability and ASDs. Subsequent database queries revealed five additional individuals with overlapping submicroscopic deletions and presenting with developmental and speech delay, seizures, behavioral abnormalities, heart defects, and dysmorphic features. The deletion found in the SCAP patient harbors ATXN1, DTNBP1, JARID2, and NHLRC1 that we propose may be responsible for ASDs and developmental delay.

  19. Theoretical study on the degradation reaction of octachlorinated dibenzo-p-dioxin with atomic oxygen O((3)P) in dielectric barrier discharge reactor.

    Gong, Chen; Sun, Xiaomin; Zhang, Chenxi; Hu, Jingtian; Qi, Chuansong

    2014-11-01

    Dielectric barrier discharges (DBD) have been used in the degradation of dioxins due to the large number of excimers and free radicals produced in discharge process. In this article, the density functional theory (DFT) is used to study the degradation mechanism of octachlorinated dibenzo-p-dioxin (OCDD) with the atomic oxygen O((3)P) in DBD reactor. The reactants, intermediates, transition states and products are optimized at the MPWB1K/6-31+G(d,p) level. The vibrational frequencies have been calculated at the same level. The reaction pathways and mechanisms are analyzed in detail. The effect of removing the chlorine atom on environment also has been discussed. PMID:25458683

  20. First observation of the strongly forbidden transition 1S0 - 3P0 in Strontium, for an atomic clock with trapped atoms

    This thesis reports the first results towards the realization of an optical clock using trapped strontium atoms. This set up would combine advantages of the different approaches commonly used to develop an atomic frequency standard. The first part describes the cold atoms source which is implemented. A magneto-optical trap operating on the 1S0-1P1 transition at 461 nm is loaded from an atomic beam decelerated by a Zeeman slower. The 461 nm laser is obtained by sum-frequency mixing in a potassium titanyl phosphate (KTP) crystal. The second part is devoted to the different stages developed to achieve the direct excitation of the 1S0-3P0 clock transition in 87Sr. This line has a theoretical natural width of 10-3 Hz. Before this detection, we obtained an estimate of the resonance frequency by measuring absolute frequencies of several allowed optical transitions. (author)

  1. Laser-induced fluorescence and polarized light in the collision regime: the He(2s 3S → 4p 3P) case

    Laser-induced fluorescence experiments carried out using linearly polarized photons from a pulsed laser on a He(2s 3S → 4p 3P) excitation-detection scheme in electrical discharges at 0.04-0.6 Torr, show that light emitted during the interaction and in the subsequent decay manifests anisotropy in its angular distribution and polarization. A rate equation approach is used to discuss the general features of the interaction between polarized light and atoms observed experimentally. The incident electromagnetic field is decomposed into multipolar components that may interact with the atom through an appropriate coupling. Defining the Einstein coefficients for each coupling, it is possible to introduce the equations for the dynamics of the atomic populations. For each direction and polarization, the temporal profile of the intensity during the transition is calculated. The theoretical prediction explains the outcome of the He experiment satisfactorily. (author)

  2. Anticorrosion properties of enamels with high B2O3-P2O5-AlPO4 content in molten aluminum

    周鸣; 李克; 孙宝德; 疏达; 王俊

    2002-01-01

    The new enamels with high H3BO3-(NH4)2 HPO4-AlPO4 content were studied, and the melting temperature of the enamels was adjusted by adding carbonate of alkali metal and alkaline earth metal. The enamels could spread slightly in the molten Al alloy observed by SEM and experiment. The components of the enamels were not detected with electron probe (EDAX-S-520) on the interface of Al alloy, but elemental Si of aluminum alloy was found in the silica-free enamels. Moreover, the components of the boron-free enamels were detected on the interface of Al alloy. The results show that the enamels with high B2O3-P2O5-AlPO4 content are resistant to the corrosion of molten Al.

  3. Self-supported Zn3P2 nanowire arrays grafted on carbon fabrics as an advanced integrated anode for flexible lithium ion batteries

    Li, Wenwu; Gan, Lin; Guo, Kai; Ke, Linbo; Wei, Yaqing; Li, Huiqiao; Shen, Guozhen; Zhai, Tianyou

    2016-04-01

    We, for the first time, successfully grafted well-aligned binary lithium-reactive zinc phosphide (Zn3P2) nanowire arrays on carbon fabric cloth by a facile CVD method. When applied as a novel self-supported binder-free anode for lithium ion batteries (LIBs), the hierarchical three-dimensional (3D) integrated anode shows excellent electrochemical performances: a highly reversible initial lithium storage capacity of ca. 1200 mA h g-1 with a coulombic efficiency of up to 88%, a long lifespan of over 200 cycles without obvious decay, and a high rate capability of ca. 400 mA h g-1 capacity retention at an ultrahigh rate of 15 A g-1. More interestingly, a flexible LIB full cell is assembled based on the as-synthesized integrated anode and the commercial LiFePO4 cathode, and shows striking lithium storage performances very close to the half cells: a large reversible capacity over 1000 mA h g-1, a long cycle life of over 200 cycles without obvious decay, and an ultrahigh rate performance of ca. 300 mA h g-1 even at 20 A g-1. Considering the excellent lithium storage performances of coin-type half cells as well as flexible full cells, the as-prepared carbon cloth grafted well-aligned Zn3P2 nanowire arrays would be a promising integrated anode for flexible LIB full cell devices.We, for the first time, successfully grafted well-aligned binary lithium-reactive zinc phosphide (Zn3P2) nanowire arrays on carbon fabric cloth by a facile CVD method. When applied as a novel self-supported binder-free anode for lithium ion batteries (LIBs), the hierarchical three-dimensional (3D) integrated anode shows excellent electrochemical performances: a highly reversible initial lithium storage capacity of ca. 1200 mA h g-1 with a coulombic efficiency of up to 88%, a long lifespan of over 200 cycles without obvious decay, and a high rate capability of ca. 400 mA h g-1 capacity retention at an ultrahigh rate of 15 A g-1. More interestingly, a flexible LIB full cell is assembled based on the as

  4. Preparation, spectroscopic investigation and antiproliferative capacity of new metal complexes of (3E)-2-(hydroxyimino)-N-P-Tolyl-3-(P-tolylimino) butanamide

    El-Tabl, Abdou Saad; Abd El-wahed, Moshira Mohamed; Abd El-Razek, Samar Ebrahim

    2013-03-01

    Cr(III), Mn(II), Fe(III), Co(II), Ni(II), Cu(II), Zn(II), Cd(II), Sr(II), Hg(II), Tl(I), UO2(II) and ZrO(II) complexes of (3E)-2-(hydroxyimino)-N-P-Tolyl-3-(P-tolylimino) butanamide have been prepared and characterized by elemental analyses, IR, UV-Vis spectra, magnetic moments, conductances, 1H NMR and mass spectra (ligand and its Zn(II) complex), thermal analyses (DTA and TGA) and ESR measurements. The IR data show that, the ligand behaves as monobasic bidentate or neutral bidentate. Molar conductances in DMF indicate that, the complexes are non-electrolytes. ESR spectra of solid Cu(II) complexes at room temperature show axial type (dx2-y2) with covalent bond character in an octahedral environment. Complexes showed inhibitory activity against hepatocellular carcinoma (HepG-2 cell line).

  5. Photoemission study of Ge1-x-y Mnx Euy Te at Mn 3p-3d and Eu 4d-4f resonances

    The electronic structure of Ge0.4 Mn0.5 Eu0.1 Te has been investigated by means of resonant photoemission spectroscopy. The spectra were acquired for the photon energy ranges of 130-155 and 40-60 eV corresponding to the Eu 4d → 4f and Mn 3p → 3d resonances, respectively. The features related to Eu 4f and Mn 3d states were revealed in the emission from the valence band. It was shown that europium ions occur as Eu2+. The Mn2+ 3d states contribute to the whole valence band with a maximum of the Mn 3d distribution at 3.9 eV

  6. Synthesis and structure of a new halophosphate Sr3P3O10Cl with the flexible [P3O10]5- anions

    Gao, Jianhua; Liu, Xi; Song, Limei; Sha, Xunshan; Zhao, Pan; Guo, Ping

    2016-05-01

    A new halophosphate crystal Sr3P3O10Cl was grown in molten chloride flux media. It crystallizes in the centrosymmetric orthorhombic space group Pnma (No. 62) with a = 10.617(2) Å, b = 10.736(2) Å, and c = 8.7354(17) Å. In the structure, the basic building unit is the [P3O10]5- anion, which is consist of three PO4 tetrahedra by sharing the corner oxygen atoms. The two Sr atoms and the Cl atom are linked to construct an infinite [Sr3Cl]5+ chain. The [P3O10]5- anions are interconnected with the [Sr3Cl]5+ chains to form a three-dimensional frameworks. Additionally, the first-principle calculation was employed to obtain the band structures and densities of states.

  7. The oxytocin receptor gene (OXTR) localizes to human chromosome 3p25 by fluorescence in situ hybridization and PCR analysis of somatic cell hybrids

    Simmons, C.F. Jr.; Clancy, T.E.; Quan, R. [Children`s Hospital, Boston, MA (United States)] [and others

    1995-04-10

    The human oxytocin receptor regulates parturition and myometrial contractility, breast milk let-down, and reproductive behaviors in the mammalian central nervous system. Kimura et al. recently identified a human oxytocin receptor cDNA by means of expression cloning from a human myometrial cDNA library. To elucidate further the molecular mechanisms that regulate oxytocin receptor gene expression and to define the expected Mendelian inheritance of possible human disease states, we must determine the number of genes, their localization, and their organization and structure. We summarize below our data indicating that the human oxytocin receptor gene is localized to 3p25 and exists as a single copy in the haploid genome. 9 refs., 2 figs.

  8. Excitation of the OI (3s 5S0-3p 5P; lambda 7774 A) multiplet by electron impact on O2

    Erdman, P. W.; Zipf, E. C.

    1987-01-01

    Electron impact on O2 has been employed to ascertain the absolute cross-section value and emission linewidths of the OI (3s 5S0-3p 5P; 7774 A) multiplet. The emission linewidths are highly Doppler-broadened in dissociative excitation, and display two distinct kinetic energy distributions: which indicate that both purely repulsive and discrete, bound, excited molecular states, which then predissociate, are involved in the dissociation process that leads to the excitation of OI 7774 A. The magnitude of the measured cross-section and the fragment kinetic energy distribution both indicate that the previous time-of-flight studies of the metastable OI (5S0) state require reinterpretation.

  9. Chronic administration of the selective P2X3, P2X2/3 receptor antagonist, A-317491, transiently attenuates cancer-induced bone pain in mice

    Hansen, RR; Nasser, A; Falk, S;

    2012-01-01

    The purinergic P2X3 and P2X2/3 receptors are in the peripheral nervous system almost exclusively confined to afferent sensory neurons, where they are found both at peripheral and central synapses. The P2X3 receptor is implicated in both neuropathic and inflammatory pain. However, the role of the ......X3 receptor in chronic cancer-induced bone pain is less known. Here we investigated the effect of systemic acute and chronic administration of the selective P2X3, P2X2/3 receptor antagonist (5-[[[(3-Phenoxyphenyl)methyl][(1S)-1,2,3,4-tetrahydro-1-naphthalenyl]amino]carbonyl]-1...

  10. Study on behavior of reinforcement in molten pool for submicron composite Al2O3p/6061Al during laser welding

    2001-01-01

    The behavior of the reinforcement of submicron composite Al2O3p/6061Al in molten pool during laser welding was studied. It was indicated that because there were the remarkable differences in thermal physical properties between matrix and reinforcement particulate, the reinforced particulate was pushed forward during molten pool solidification by the liquid-solid interface in matrix and the reinforced particulate segregated in the weld. It resulted in noticeable degradation in properties of the welded joint. The technology methods to improve the behavior of reinforcement were also investigated. It was pointed out that the laser pulse frequency is the main welding parameter affecting the distributive state of reinforcement, and the theory basis was established for welding the material by laser beam.

  11. Quasi-classical Trajectory Study of Reaction O (3P) + HCl (v = 2; j = 1,6,9) → OH + Cl

    The reaction O (3P) + HCl (v = 2; j = 1,6,9) → OH+ Cl is theoretically studied with a quasi-classical trajectory method (QCT) on the benchmark potential energy surface of the ground 3A'' state [J. Chem. Phys. 119(2003)9550]. The QCT-calculated state-resolved rotational distributions are in good agreement with the experimental results. The rotational polarization of the product OH molecule becomes weaker as the initial HCl rotation is excited. The calculated results can be explained from the large mass factor cos2 β of the title reaction, the van der Waals well in the potential energy surface and the secondary encounters in the exit channel. (atomic and molecular physics)

  12. Properties and Structure of RO-R2O-Na2O-Al2O3-P2O5 Glasses

    The properties and structural features of RO-R2O-Na 2O-Al2O3-P2O5 (R= Mg, Ca, Ba and RO= Li) glasses in the system have been investigated. The properties of those glasses seem to depend on the field strength of the alkali or alkali-earth ions in glasses as in silicate glasses. Infrared (IR) spectroscopy indicates that the glass network is dominated by bridging P-tetrahedral present in glasses with O/P ≅ 3.0. The variation in physical properties of the glasses seem to be closely related to the variation in structure of the glasses and could be explained by simple mechanism such as field strength and differences in mass

  13. Two genetic pathways, t(1;10) and amplification of 3p11-12, in myxoinflammatory fibroblastic sarcoma, haemosiderotic fibrolipomatous tumour, and morphologically similar lesions.

    Hallor, Karolin H; Sciot, Raf; Staaf, Johan; Heidenblad, Markus; Rydholm, Anders; Bauer, Henrik Cf; Aström, Kristina; Domanski, Henryk A; Meis, Jeanne M; Kindblom, Lars-Gunnar; Panagopoulos, Ioannis; Mandahl, Nils; Mertens, Fredrik

    2009-04-01

    Myxoinflammatory fibroblastic sarcoma (MIFS) is a low-grade malignant neoplasm for which limited genetic information, including a t(1;10)(p22;q24) and amplification of chromosome 3 material, is available. To further characterize these aberrations, we have investigated eight soft tissue sarcomas diagnosed as MIFS, haemosiderotic fibrolipomatous tumour (HFT), myxoid spindle cell/pleomorphic sarcoma with MIFS features, and inflammatory malignant fibrous histiocytoma/undifferentiated pleomorphic sarcoma with prominent inflammation (IMFH) harbouring a t(1;10) or variants thereof and/or ring chromosomes with possible involvement of chromosome 3. Using chromosome banding, fluorescence in situ hybridization, array-based comparative genomic hybridization, global gene expression, and real-time quantitative PCR analyses, we identified the breakpoint regions on chromosomes 1 and 10, demonstrated and delineated the commonly amplified region on chromosome 3, and assessed the consequences of these alterations for gene expression. The breakpoints in the t(1;10) mapped to TGFBR3 in 1p22 and in or near MGEA5 in 10q24, resulting in transcriptional up-regulation of NPM3 and particularly FGF8, two consecutive genes located close to MGEA5. The ring chromosomes contained a commonly amplified 1.44 Mb region in 3p11-12, which was associated with increased expression of VGLL3 and CHMP2B. The identified genetic aberrations were not confined to MIFS; an identical t(1;10) was also found in a case of HFT and the amplicon in 3p was seen in an IMFH. PMID:19199331

  14. Quercetin induces hepatic γ-glutamyl hydrolase expression in rats by suppressing hepatic microRNA rno-miR-125b-3p.

    Wein, Silvia Anette; Laviano, Alessandro; Wolffram, Siegfried

    2015-12-01

    Exogenous factors such as food components including the flavonoid quercetin are suspected to influence micro RNA (miRNA) concentrations and thus possibly target enzymes involved in xenobiotic metabolism. This study therefore investigates the influence of orally administered quercetin on hepatic miRNA and the identification of enzyme target mRNAs relevant in drug metabolism. Male Wistar rats (n=16) were fed either a diet without (C) or with (Q) the addition of 100-ppm quercetin for 7 weeks and subsequently euthanized at the end of the dark phase. To avoid strong effects of food deprivation on hepatic metabolism, food was not removed until 5 h prior to the procedure. Liver was immediately dissected and snap-frozen in liquid nitrogen. Concentrations of 352 hepatic miRNA were measured in pool samples of each dietary group (n=8) using the RT(2) miRNA PCR Array System. Differential expression of miRNAs was assumed with fold changes ≥3. Target genes of differentially expressed miRNAs were identified using the database TargetScan. Because rno-miR-125b-3p showed the most prominent fold-change (-9) we further analyzed the expression of its top predicted target gene gamma-glutamyl hydrolase (GGH) by quantitative real-time PCR using hypoxanthine phosphoribosyltransferase 1 (hprt1) as endogenous control. Compared to controls, 23 miRNAs were differentially expressed in rats fed quercetin. A ninefold reduction in hepatic miRNA rno-miR-125b-3p was paralleled by significant induction of GGH mRNA in liver of quercetin fed rats. Because increased GGH expressions were repeatedly associated with resistance to methotrexate, concomitant intake with quercetin should be monitored carefully. PMID:26432773

  15. An Experimental and Theoretical Investigation of the C((1)D) + N2 → C((3)P) + N2 Quenching Reaction at Low Temperature.

    Hickson, Kevin M; Loison, Jean-Christophe; Lique, François; Kłos, Jacek

    2016-04-28

    The gas-phase quenching reaction C((1)D) + N2 → C((3)P) + N2 has been investigated experimentally over the temperature range 50-296 K using a supersonic flow reactor. C((1)D) atoms were produced in situ by the pulsed multiphoton dissociation of CBr4 precursor molecules. Rate constants for this process were measured using a chemical tracer method whereby the C((1)D) + H2 → CH + H reaction was employed to follow C((1)D) decays by monitoring vacuum ultraviolet laser-induced fluorescence of the atomic hydrogen product at 121.567 nm. The deactivation rates are seen to increase at lower temperature, indicating the likely influence of the CNN intermediate complex lifetime on intersystem crossing for this system. We also performed electronic structure calculations of relevant C((3)P)-N2 and C((1)D)-N2 potential energy curves as well as triplet-singlet spin-orbit coupling terms using the explicitly correlated internally contracted multireference configuration interaction method (ic-MRCI-F12). The calculations were performed for the collinear and perpendicular approach of the C atom toward the N2 molecule, which allowed us to construct the approximate spherical (isotropic) potential model of C-N2(j = 0). The computed reduced dimensional potentials were used in quantum close coupling scattering calculations of the electronic quenching cross sections and rate constants. While the calculated potential energy curves form the basis for a good qualitative description of the reaction, the calculated rate constants are significantly smaller than the measured ones, and fail to reproduce the temperature dependence of the experimental results. Several possible reasons are provided to explain the origin of these differences. PMID:27046417

  16. Noble-gas broadening rates for the 6s2S0→6s6p 1,3P1 resonance and intercombination lines of barium

    The collisional-broadening and -shift rate coefficients (kbr and kshift), for both helium and argon buffer gases, have been measured in the impact limit for the 6s21S0→6s6p 3P1 intercombination transition of barium. Detailed absorption line shapes were measured at three different ground-state number densities by monitoring the transmitted intensity of a weak cw probe laser as it was scanned over the transition. A single, density-independent line shape was then constructed and fit to a sum of Voigt functions (to account for hyperfine structure and isotope shifts) in order to determine the broadening and shift rates. By repeating the process for several buffer-gas pressures, kbr and kshift were determined from least-squares straight-line fits to the broadening and shift rates versus buffer-gas number density. Absolute normalization of the line-shape data was used to provide accurate ground-state column densities. Column-density information was then combined with measurements of white-light-absorption equivalent widths for the 6s21S0→6s6p 1P1 resonance transition to yield broadening rates for that transition. These values for the resonance line lie between previous literature values, which are widely discrepant. Finally we measured the absolute metastable 6s5d 3D2 column density under conditions of optical pumping through the 3P1 level, and determined that, in steady state, even with a weak cw pump laser, ∼20% of all barium atoms in the vapor could be found in the 3D2 level (with approximately 60% spread over the full 3DJ metastable manifold)

  17. Two isotypic diphosphates Li M2H 3(P 2O 7) 2 ( M=Ni, Co) containing ferromagnetic zigzag MO 6 chains

    Yang, Tao; Yang, Sihai; Liao, Fuhui; Lin, Jianhua

    2008-06-01

    Two new isotypic phosphates LiNi 2H 3(P 2O 7) 2 ( 1) and LiCo 2H 3(P 2O 7) 2 ( 2) have been hydrothermally synthesized and structurally characterized by the single-crystal X-ray diffraction technique. They crystallize in the monoclinic space group C2/ c with the lattice: a=10.925(2) Å, b=12.774(3) Å, c=8.8833(18) Å, β=123.20(3)° for 1 and a=10.999(2) Å, b=12.863(3) Å, c=8.9419(18) Å, β=123.00(3)° for 2. The transition metal atoms are octahedrally coordinated, whereas the lithium and phosphorus atoms are all tetrahedrally coordinated. As the lithium-induced derivatives of MH 2P 2O 7 ( M=Ni, Co), 1 and 2 possess the same structure with MH 2P 2O 7 in terms of topology, comprising the MO 6 zigzag chains and P 2O 7 as the interchain groups. The magnetisms of 1 and 2 could be interpreted by adopting a quasi-one-dimensional (1D) zigzag chain model as that in their parent compounds: both 1 and 2 have ferromagnetic (FM) NiO 6/CoO 6 chains; 1 shows a FM cluster glass behavior at low temperatures, which is originated from the possible antiferromagnetic (AFM) next-nearest-neighbour intrachain interactions; 2 shows a AFM ordering at TN=2.6 K and a metamagnetic transition at HC=4.2 kOe at 1.8 K.

  18. Experimental and theoretical studies of the O(3P) + C2H4 reaction dynamics: collision energy dependence of branching ratios and extent of intersystem crossing.

    Fu, Bina; Han, Yong-Chang; Bowman, Joel M; Leonori, Francesca; Balucani, Nadia; Angelucci, Luca; Occhiogrosso, Angela; Petrucci, Raffaele; Casavecchia, Piergiorgio

    2012-12-14

    The reaction of O((3)P) with C(2)H(4), of importance in combustion and atmospheric chemistry, stands out as paradigm reaction involving not only the indicated triplet state potential energy surface (PES) but also an interleaved singlet PES that is coupled to the triplet surface. This reaction poses great challenges for theory and experiment, owing to the ruggedness and high dimensionality of these potentials, as well as the long lifetimes of the collision complexes. Crossed molecular beam (CMB) scattering experiments with soft electron ionization detection are used to disentangle the dynamics of this polyatomic multichannel reaction at a collision energy E(c) of 8.4 kcal∕mol. Five different primary products have been identified and characterized, which correspond to the five exothermic competing channels leading to H + CH(2)CHO, H + CH(3)CO, CH(3) + HCO, CH(2) + H(2)CO, and H(2) + CH(2)CO. These experiments extend our previous CMB work at higher collision energy (E(c) ∼ 13 kcal∕mol) and when the results are combined with the literature branching ratios from kinetics experiments at room temperature (E(c) ∼ 1 kcal∕mol), permit to explore the variation of the branching ratios over a wide range of collision energies. In a synergistic fashion, full-dimensional, QCT surface hopping calculations of the O((3)P) + C(2)H(4) reaction using ab initio PESs for the singlet and triplet states and their coupling, are reported at collision energies corresponding to the CMB and the kinetics ones. Both theory and experiment find almost an equal contribution from the triplet and singlet surfaces to the reaction, as seen from the collision energy dependence of branching ratios of product channels and extent of intersystem crossing (ISC). Further detailed comparisons at the level of angular distributions and translational energy distributions are made between theory and experiment for the three primary radical channel products, H + CH(2)CHO, CH(3) + HCO, and CH(2) + H(2)CO

  19. Delayed emission of 2p-1s and 3p-1s X-rays from 40 MeV neon ions ionized in a thin carbon foil

    A previous study of the He- and H-like lines of 40 MeV Ne projectiles excited by a thin carbon foil showed that the x-ray spectrum contains only He-like and H-like lines and that the intensities of these lines are sufficient to employ a high resolution curved crystal spectrometer to study the decay curves for individual transitions. Presented are Ne K x-ray spectra excited by passing a beam of 40 MeV ions through a 50 μg/cm2 carbon foil. They were taken at 0, 2.3, and 9.1 mm behind the foil and are normalized to the same total integrated charge in the carbon foil. The 1s2p(23P1)-1s2(11S0) transition is a spin-forbidden, metastable, intercombination line and has a lifetime of 1.85 x 10-10 sec8, whereas the He-like 1s2p(1P)-1s2(1S), H-like 2p(2P)-1s(2S), and H-like 3p(2P)-1s(2s) transitions are dipole-allowed with lifetimes of 1.13 x 10-13 sec8, 1.59 x 10-13 sec9, and 5.98 x 10-13 sec 10, respectively. It is apparent that even at 1 cm (i.e. 0.5 ns) behind the foil, considerable intensity is still detected for the short lifetime transitions indicating that electrons are cascading from very high n levels. Previous measurements and calculations have shown that the decay curves can be described by a power law of the form I = ct/sup -b/, where c and b are constants for a given line. It has been found that b depends on the initial n,l distribution. The measured decay curves for the delayed He-Like 1s2p(1P)-1s2(1S), H-like 2p-1s and H-like 3p-1s transitions are shown fitted to this power law

  20. Study on the Two-photon Resonance-enhanced Multiphoton Ionization of CF Radicals:Rotational Analysis of 3p Rydberg State%CF自由基双光子共振增强多光子电离研究:3pRy dberg态的转动分析

    李全新; 束继年; 张群; 周晓国; 陈从香; 俞书勤; 马兴孝

    2000-01-01

    利用Ar/CF4、Ar/CF2Cl2或Ar/CF3COOH混合气体的直流脉冲放电产生CF自由基,观测了260~360nm范围内转动分辨的CF自由基双光子共振增强多光子电离谱。分析表明,该段光 谱对应于CF自由基3pπD2Πr(ν'=2~6,r=3/2,1/2)←←X2Πr(ν"=0,r=3/2,1/2)的共振激发。对观测的振动带进行了转动分析,并获得了3p里德堡态的转动常数和自旋-轨道分裂值。%CF radicals were generated by using a dc discharge in the mixture of Ar/CF4,Ar/CF2Cl2,and Ar/CF3COOH.The rotationally resolved(2+1)resonance-enhanced multiphoton ionization(REMPI)spectra of CF radical have been investigated in the wavelength range 260~360nm.The analysis shows that the observed spectra can be assigned as the two-photon resonance excitation 3pπD2Πr(ν'=2~6,r=3/2,1/2)←←X2Πr(ν"=0,r=3/2,1/2).The related rotational constants and the spin-orbit splitting values of CF 3p Rydberg state have been derived.