International Nuclear Information System (INIS)

The Food Safety and Inspection Service (FSIS) of the United States Department of Agriculture (USDA) inspects domestic and imported meat and poultry food products to assure the public that they are safe, wholesome, not economically adulterated and properly labeled. The Service also monitors the activities of meat and poultry plants and related activities in allied industries, and establishes standards and approves labels for meat and poultry products. As part of its responsibility, shortly after the Chernobyl accident occurred, FSIS developed a plan to assess this accident's impact on domestically produced and imported meat and poultry

Energy Technology Data Exchange (ETDEWEB)

Copper bioavailability was studied in rats using an extrinsic Cu label. Copper absorption from sunflower seeds (46%), peanuts (41%), cooked shrimp (50%), and cooked beef (40%) was as good or better than copper sulfate (46%). Copper from plant foods (sunflower seeds, garbanzo beans, peanuts) was absorbed equally as well as copper from animal foods (beef, shrimp, chicken liver), 39 +/- 7% vs 43 +/- 7%, P greater than 0.05. There was no significant difference in percentage Cu absorption between intrinsically labeled chicken liver and extrinsically labeled chicken liver. In a second experiment, Cu absorption was measured in the presence of glucose, fructose, sucrose, or cornstarch. There were no significant differences in Cu absorption due to different carbohydrates in a single meal.

British Library Electronic Table of Contents (United Kingdom)

Program decisions by symphony orchestra management are influenced by various factors. To examine these factors, we create an objective index of the propensity of a symphony orchestra to perform the standard repertoire. We use regression analysis to examine factors that influence programming decisions of 64 US symphony orchestras in 2006?2007, including public and private sources of funding. We find that increased funding from ticket sales, endowments, and local government increases the likelihood that an orchestra will perform nonstandard repertoire. In addition, the results suggest that a symphony orchestra?s music director does not have a significant impact on the degree of program conventionality.

CERN Document Server

This thesis describes hadron reconstruction at the Compact Muon Solenoid (CMS) experiment at the Large Hadron Collider (LHC) at CERN, Geneva. The focus is on the particle flow reconstruction of these objects. This thesis revisits the subject of the CMS calorimeters' non-linear response to hadrons. Data from testbeam experiments conducted in 2006 & 2007 is compared with simulations and substantial differences are found. A particle flow calibration to correct the energy response of the testbeam data is evaluated. The reconstructed jet response is found to change by ~ 5% when a data-driven calibration is used in place of the calibration derived from simulation. Collision data taken at the early stage of CMS' commissioning is also presented. The hadron response in data is determined to be compatible with testbeam results presented in this thesis. This thesis also details the use of neural networks to improve the energy measurement of hadrons at ...

Energy Technology Data Exchange (ETDEWEB)

The use of ionising radiation for the preservation of food has been under investigation for many years but has yet to receive worldwide acceptance. Although irradiation can be carefully controlled, it is generally accepted that the development of a test or tests for the detection of irradiated food would enhance consumer confidence and might help to enforce labelling regulations. The 2-alkylcyclobutanones are reported to be the only cyclic compounds formed as the products of the radiolysis of saturated and unsaturated triglycerides. The synthesis of 2-dodecylcyclobutanone (DCB) and 2-tetradecylcyclobutanone (TCB), which are formed from palmitic and stearic acid respectively following irradiation, has been carried out and using irradiated chicken meat as the model for a high-lipid containing food, both cyclobutanones have been extracted with hexane and then identified using gas chromatography/mass ...

International Nuclear Information System (INIS)

Immunoassays are versatile analytical techniques that had a leading role in various clinical applications, during the last four decades. The studies carried out by Rosalyn Yalow, Solomon Berson and Roger Ekins in the 1960s gave a breakthrough in the development of this novel analytical method. Sensitivity up to femtomolar concentrations, high specificity and universal application to different classes of molecular species made immunoassay a very useful tool in analytical investigation. The expertise acquired by immunochemists in producing antibodies against any antigen and the ability of radiochemists in labeling the antigens with "1"2"5I without affecting the active site are the two main factors responsible for the above development. There are concerns about the safety and health of humans due to the high-level contamination of environment by pesticides, industrial compounds and metals, anabolic steroids in milk and meat products, and presence of mycotoxins in ...

Wastenet

...and supply ensuring better food quality for the consumer developing safer and more flexible food production processes and technologies raw materials for food production; production and processing systems; by-products and wastes from fisheries and agro-industry; new food sources; packaging systems; quality monitoring; traceability Food contamination detecting and eliminating infectious and toxic agents throughout the food chain establishing the hazards and origins of food contaminants producing food more safely rapid ...

Science.gov (United States)

Vildagliptin is a potent and selective dipeptidyl peptidase IV inhibitor in development for the treatment of type 2 diabetes that improves glycemic control by enhancing alpha- and beta-cell responsiveness to glucose. Two open-label, single-dose, randomized, crossover studies in healthy subjects (ages 18-45 years) investigated the dose proportionality of vildagliptin pharmacokinetics (n = 20) and the effect of food (n = 24) on vildagliptin pharmacokinetics. There was a linear relationship (r(2) = 0.999) between vildagliptin doses of 25, 50, 100, and 200 mg and area under the plasma concentration-time curve from time zero to infinity (AUC(0-infinity)) and maximum plasma concentration (C(max)). Dose proportionality was assessed using a statistical power model [X = alpha x (dose)(beta)]. The 90% confidence intervals of the proportionality coefficient, beta, for AUC(0-infinity) (1.15-1.19) and C(max) (1.04-1.14) indicated that deviations from dose ...

Science.gov (United States)

... of waste food generated at McMurdo Station. Some of the difficulties of disposing of waste food ... change waste generation or management at the site? Yes. This will be a temporary waste management ...

Science.gov (United States)

The report contains detailed information with regard to the data collected in the 1977 Survey of Industry on the Use of Food Additives undertaken by committees of the Food and Nutrition Board of the National Research Council under sponsorship of the Food ...

Science.gov (United States)

... Accession Number : ADA145145. Title : Regulation of Cancer-Causing Food Additives-Time for a Change? Corporate Author ...

International Nuclear Information System (INIS)

A brief article examines the controversy over food irradiation regarding the wholesomeness of irradiated food, its microbiological safety, loss of vitamins and changes in flavour. The benefits of food irradiation are also outlined including the destruction of certain food-borne pathogens and the prolongation of the shelf-life of food by killing pests and delaying the deterioration process. (UK).

Energy Technology Data Exchange (ETDEWEB)

Brief item.

Science.gov (United States)

Although Bois noir is one of the main phytoplasma diseases of grapevine, the gene expression and enzyme activities that underlie physiological changes occurring in symptomatic and recovered (with spontaneous or induced symptom remission) plants are mostly unknown. Bois noir symptomatic leaves (September 2006, 2007) and symptomless leaves from infected symptomatic plants (September 2007) of Sangiovese (moderately susceptible) and Chardonnay (highly susceptible) cultivars were collected. Moreover, leaves from infected symptomless plants of both cultivars were harvested in June 2007. Leaves from recovered plants were also collected in the same periods. In recovered plants of both cultivars, class III chitinase and almost every time phenylalanine ammonia-lyase and chalcone synthase expression were increased for all collection periods. In symptomatic leaves of both cultivars, the expressions of the same genes were up-regulated and also those of ...

International Nuclear Information System (INIS)

This paper provided an outline of the Canadian natural gas commodity market and a forecast for Canadian natural gas supply and prices for the 2007 winter heating season. Since 2001, steady demand growth and slow supply growth have contributed to higher North American natural gas commodity prices. The loss of natural gas supply due to hurricanes pushed North American natural gas commodity prices to record levels in 2005. Canadian natural gas production levels currently exceed domestic gas consumption. In 2005, Canada produced 16.5 billion cubic feet per day of marketable natural gas. Approximately 45 per cent was consumed domestically, and the remainder was exported to the United States. Despite high levels of drilling activity, production of natural gas from western Canada has flattened out since 2001. In 2006, natural gas prices in Canada declined due to the restoration of United States Gulf Coast natural gas production; the absence of any major hurricanes; and a mild winter which ...

British Library Electronic Table of Contents (United Kingdom)

The National Food and Nutrient Analysis Program (NFNAP) was implemented in 1997 to update and improve the quality of food composition data maintained by the United States Department of Agriculture (USDA). NFNAP was designed to sample and analyze frequently consumed foods in the U.S. food supply using statistically rigorous sampling plans, established sample handling procedures, and qualified analytical laboratories. Methods for careful handling of food samples from acquisition to analysis were developed to ensure the integrity of the samples and subsequent generation of accurate nutrient values. The infrastructure of NFNAP, under which over 1500 foods have been sampled, mandates tested sample handling protocols for a wide variety of foods. The majority of these foods were categorized into ...

Energy Technology Data Exchange (ETDEWEB)

The microbial safety of foods cooked in microwave ovens was investigated. The mechanisms of microwave destruction of microorganisms were examined. Effects of time and temperature on microorganisms in different food systems were described. Studies showed that: microwave heating of food is more ''''food dependent'' than conventional heating; recommended microwave treatment time for some foods may not destroy high levels of bacteria; use of microwaves in combination with conventional heating methods results in more uniform heating of foods and destruction of bacteria; and microwaves exert different killing effects on individual bacterial species. (78 references, 2 tables)

UK PubMed Central (United Kingdom)

Factors affecting the membrane filtration of food suspensions were studied for 58 foods and 13 membrane filters. Lot number within a brand, pore size (0.45 or 0.8 micrometer), and time elapsed before...Full Text Available

UK PubMed Central (United Kingdom)

Female mammals are particularly sensitive to changes in food availability. The mechanisms that affect sexual behavior and food intake are closely related to one another; chief among the mechanisms...Full Text Available

Wastenet

...5% of foods exceed the maximum pesticide residues level , EFSA says Monday, 12 July 2010 12:41 European foods are largely under the ...Maximum Pesticide Residues Level , according to EFSA: in 2008 only 3.5% of analysed samples exceeded the MRLs (4.2% ...

International Nuclear Information System (INIS)

Requirements for food packaging materials include whether there is any interaction between the food and the package during or after the irradiation, and whether as a result of the irradiation, volatile or leachable substances are released from the pack into the food. The performance of cellulose-based materials and plastic films under irradiation are discussed.

Science.gov (United States)

... preferentially settled in habitats with supplemental food, only male meadow voles showed a settlement pattern significantly different from that ... ...

Science.gov (United States)

This layer represents the different food storage facilities available at backcountry campsites along the coast of Kenai Fjords National Park. Site locations ... ...

British Library Electronic Table of Contents (United Kingdom)

To determine whether PKH26 labeling affects the morphologies, phenotypes, proliferation, and secretion abilities of human umbilical mesenchymal stromal cells (HUMSCs) were investigated. Isolated HUMSCs were labeled with PKH26, and cell morphology was observed under microscope. Cell cycle, apoptotic cell death, expression of PKH26, and the proliferation rate were evaluated. Additionally, fluorescence intensity of PKH26 labeling at different passage times was quantified. There were no detectable differences in cell morphology, cell growth, and proliferation rate after PKH26 labeling. In addition, fluorescence intensity of PKH26 labeling was gradually reduced with increase of the passage times. The PKH26 labeling disappeared after passage six times. In summary, PKH26 labeling is a safe and ef...

KoreaScience (Korea)

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KoreaScience (Korea)

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International Nuclear Information System (INIS)

This bibliography contains citations concerning the migration of food-packaging materials into foods. Plastic, glass, cardboard, metal, and ceramic containers are discussed. Techniques for analyzing packaging contamination are included. (Contains 90 citations fully indexed and including a title list.).

KoreaScience (Korea)

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CERN Document Server

A new method of food stuff energetic value scoring is offered

Wastenet

... Agriculture Secretary Tom Vilsack wants his agency to \\

KoreaScience (Korea)

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International Nuclear Information System (INIS)

Joint FAO/IAEA/WHO Expert Committee approved the use of radiation treatment of foods. Nowadays food packaging are mostly made of plastics, natural or synthetic, therefore effect of irradiation on these materials is crucial for packing engineering for food irradiation technology. By selecting the right polymer materials for food packaging it can be ensured that the critical elements of material and product performance are not compromised. When packaging materials are in contact with food at the time of irradiation that regulatory approvals sometimes apply. The review of the R-and-D and technical papers regarding material selection, testing and approval is presented in the report. The most information come from the USA where this subject is well elaborated, the International Atomic Energy Agency (IAEA) reports are reviewed as well. The report can be useful for scientists and ...

Science.gov (United States)

Background Food additives are an integral part of the modern food system, but opinion polls showing most Europeans have worries about them imply an urgent need for ethical analysis of their use. Sources of data The existing literature on food ethics, safety assessment and animal testing. Areas of agreement Food additives provide certain advantages in terms of many people's lifestyles. Areas of controversy There are disagreements about the appropriate application of the precautionary principle and of the value and ethical validity of animal tests in assessing human safety. Growing points Most consumers have a poor understanding of the relative benefits and risks of additives, but concerns over food safety and animal testing remain high. Areas timely for developing research Examining the impacts of food additives on consumer sovereignty, consumer health and on ...

Science.gov (United States)

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Science.gov (United States)

This library, written in Java, supports input and output of images and metadata (labels) in the

International Nuclear Information System (INIS)

In the present study the authors have shown that glycoproteins are present in the plasma membrane of Acanthamoeba castellanii by utilizing different radioactive labeling techniques. Plasma membrane proteins in the amoeba were iodinated by "1"2"5I-lactoperoxidase labeling and the solubilized radiolabeled glycoproteins were separated by lectin-Sepharose affinity chromatography followed by polyacrylamide gel electrophoresis. The periodate/NaB"3H_4 and galactose oxidase/NaB"3H_4 labeling techniques were used for labeling of surface carbohydrates in the amoeba. Several surface-labeled glycoproteins were observed in addition to a diffusely labeled region with M_r of 55,000-75,000 seen on electrophoresis, which could represent glycolipids. The presence of glycoproteins in the plasma membrane of Acanthamoeba castellanii was confirmed by metabolic ...

International Nuclear Information System (INIS)

The term 'health/functional food' (HFF) refers to food supplements containing nutrients or other substances (in a concentrated form) that have a nutritional or physiological effect whose purpose is to supplement the normal diet. The Korean Health/Functional Food Act that came into effect in 2004 requires these products to be marketed in measured doses, such as in pills, tablets, capsules, and liquids. HFFs are of two types: generic and product-specific. There are 37 ingredients listed in the act for generic HFFs, and if an HFF contains a new active ingredient that is not included in the generic 37 products, it is considered a product-specific HFF. The standardization, safety, and efficacy of a new active ingredient are reviewed by the Korean Food and Drug Administration in order to receive approval as a product-specific HFF. Conforming with international standards and protecting public health requires ...

International Nuclear Information System (INIS)

This discussion paper has two goals: first, to raise public awareness of food irradiation, an emerging technology in which Canada has the potential to build a new industry, mainly oriented to promising overseas markets; and second, to help build consensus among government and private sector decision makers about what has to be done to realize the domestic and export potential. The following pages discuss the potential of food irradiation; indicate how food is irradiated; outline the uses of food irradiation; examine questions of the safety of the equipment and both the safety and nutritional value of irradiated food; look at international commercial developments; assess the current and emerging domestic scene; and finally, draw some conclusions and offer suggestions for action.

British Library Electronic Table of Contents (United Kingdom)

Pesticides are widely used in food production to increase food security despite the fact that they can have negative health effects on consumers. Pesticide residues have been found in various fruits and vegetables; both raw and processed. One of the most common routes of pesticide exposure in consumers is via food consumption. Most foods are consumed after passing through various culinary and processing treatments. A few literature reviews have indicated the general trend of reduction or concentration of pesticide residues by certain methods of food processing for a particular active ingredient. However, no review has focused on combining the obtained results from different studies on different active ingredients with differences in experimental designs, analysts and analysis equipment. In...

Science.gov (United States)

BackgroundIn recent years, near-infrared fluorescence (NIRF)-labeled iron nanoparticles have been synthesized and applied in a number of applications, including the labeling of human cells for monitoring the engraftment process, imaging tumors, sensoring the in vivo molecular environment surrounding nanoparticles and tracing their in vivo biodistribution. These studies demonstrate that NIRF-labeled iron nanoparticles provide an efficient probe for cell labeling. Furthermore, the in vivo imaging studies show excellent performance of the NIR fluorophores. However, there is a limited selection of NIRF-labeled iron nanoparticles with an optimal wavelength for imaging around 800 nm, where tissue autofluorescence is minimal. Therefore, it is necessary to develop additional alternative NIRF-labeled iron nanoparticles for application in this area.ResultsThis study ...

UK PubMed Central (United Kingdom)

Mutation assay with Salmonella typhimurium enabled us to detect various types of mutagens in cooked foods. A series of mutagenic heterocyclic amines has been isolated and identified in broiled fish...Full Text Available

International Nuclear Information System (INIS)

Processing of food with ionizing radiation is a method suitable to enhance shelf-life and hygienic quality. Up to a dose of 10 kGy the method is considered wholesome. In many countries the practical use of food irradiation is increasing, however, in the Federal Republic of Germany the process is strictly forbidden. Applications and methods for radiation processing of food are compiled, limits and prospects are explained, and advantages and disadvantages are compared with traditional methods. Identification of irradiated foods and dosimetry and process control for radiation processing of food are areas where further research is needed. Continuous processing of particulate foods in bulk is an application where electron accelerators might be profitable. Beam parameters and velocity distribution of food particles in the treatment area can be ...

Science.gov (United States)

... As noted by Emma Rothschild: ... 24. Emma Rothschild, "Food Politics," Foreign Affairs, 54 (January 1976),285·86. 25. Forecast 90, pp. I1·31, 32. 26. ...

Science.gov (United States)

This paper summarizes the current scientific and technological knowledge about greenhouse gas emissions from various agricultural practices and the manufacturing of food. The study also provides estimates that compare agriculture-related alternatives for reducing greenhouse gas emissions.

UK PubMed Central (United Kingdom)

In response to reports that the contamination of food can occur during the on-farm primary phase of food production, we report data that describes a possible cost-effective intervention measure. The...Full Text Available

Science.gov (United States)

Feb 25, 2008 ... NASA's Visible Earth catalog of NASA images and animations of our home planet.

Wastenet

... Do you certify Halal or Kosher meat? What fish feed do you allow? What levels of polychlorinated biphenyls (PCBs) have been found in organic fish? How can I be sure that organic products from abroad are really organic? Do you prosecute businesses that break the rules? Does the Soil Association carry out any routine testing of food? Do you test for GM material? Why do you allow additives in organic food? Is organic food fortified with vitamins and minerals? Why choose organic during pregnancy?...

International Nuclear Information System (INIS)

... Safety Institute, GRS, Cologne (Germany) INIS-UA--089 456 p. APPLIED LIFE

International Nuclear Information System (INIS)

Treatment of food with low doses of gamma radiation has been recognized to have two main advantages. These consist of: (1) improvement of food safety by elimination of pathogens and (2) reduction of microbial spoilage and extension of shelf life of perishable items by reducing the number of viable spoilage organisms. Studies during the last few decades have conclusively proved the beneficial effects of radiation with respect to fishery products. The three potential areas of application to fish products include: (i) radurization for shelf life extension (ii) radicidation to eliminate food borne pathogens in the products and (iii) radiation treatment to dried products to control insects.

KoreaScience (Korea)

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UK PubMed Central (United Kingdom)

The purpose of this study was to determine if a cannabinoid CB₁ receptor antagonist would selectively decrease consumption of highly palatable food in non-human primates. The CB₁...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

AGROSTER Co irradiates food packaging material and some types of spices for the meat industry. The step by step strategy of AGROSTER has been very successful. In the last year, its gamma facility was used as much as possible to irradiate food items. The government of Hungary has recognized the benefit of this technology and has given financial assistance to establish a large commercial gamma irradiator in Budapest.

UK PubMed Central (United Kingdom)

High resolution structural elucidation of macromolecular structure by solid-state nuclear magnetic resonance requires the preparation of uniformly aligned samples that are isotopically labeled. In...Full Text Available

CERN Document Server

We describe a clustering method for labeled link network (semantic graph) that can be used to group important nodes (highly connected nodes) with their relevant link's labels by using PARAFAC tensor decomposition. In this kind of network, the adjacency matrix can not be used to fully describe all information about the network structure. We have to expand the matrix into 3-way adjacency tensor, so that not only the information about to which nodes a node connects to but by which link's labels is also included. And by applying PARAFAC decomposition on this tensor, we get two lists, nodes and link's labels with scores attached to each node and labels, for each decomposition group. So clustering process to get the important nodes along with their relevant labels can be done simply by sorting the lists in decreasing order. To test the method, we construct ...

UK PubMed Central (United Kingdom)

Electron spin resonance (ESR) studies have been performed on spin-labeled model membranes aligned using the isopotential spin-dry ultracentrifugation (ISDU) method of Clark and Rothschild. This method...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundGenotyping analysis using capillary DNA sequencing with fluorescently labeled primer pairs obtained by polymerase chain reaction (PCR) is widely used, but is expensive....Full Text Available

International Nuclear Information System (INIS)

The basic approach to radioactive labeling techniques in hemoglobin identification consists of mixing 14C labeled normal carrier globin with 3H labeled unknown globin and carrying out the subsequent separations and purifications looking at the distribution of 3H and 14C radioactivity. This technique was devised to ascertain the amino acid substitution in Hb Indianapolis and was instrumental in solving the structure of Hb Vicksburg. It was also useful in determining the purity of gamma-chains from cultures of human and baboon erythroid progenitors. Another use of this technique would be in identifying aberrant peaks of radioactivity in chain separation chromatograms, such as ''pre-peaks''.

International Nuclear Information System (INIS)

Astatine conversed into cationic form is shown to form stable complex with diethylenetriaminpentacetic acid. Due to this complex astatine joins RN_2 type monoclonal antibodies. More favorable conditions to prepare astatine labelled antibodies are found. Chromatographical analysis and electromigration experiments have shown that astatine is strongly retained in in-vitro biomolecule. Astatine did not escape from the labelled antibodies even in case of urea effect on them. Immune activity of astatine labelled antibodies remained similar in 20 h. 28 refs., 4 figs.

Energy Technology Data Exchange (ETDEWEB)

Short communication.

Energy Technology Data Exchange (ETDEWEB)

A useful method for preparing radioiodinated photoaffinity labels from alkyl anilines which offer significant advantages over present methods is described. The one-pot synthesis gives good radiochemical yields (40-64%) of pure, high specific activity (350-1500 mCi/{mu}mol) {sup 124}I labelled iodaryl azides while minimising manipulation of radioactive materials. Purification of the ({sup 125}I)iodoazido photoaffinity labels is achieved by high performance liquid chromatography. (author).

British Library Electronic Table of Contents (United Kingdom)

Certified reference materials (CRMs) play a critical role in validating the accuracy of nutrient data for food samples. A number of available food CRMs of differing matrix composition have assigned concentrations for various nutrients, along with associated uncertainty intervals (UIs) for those values. These CRMs have been used extensively in the United States Department of Agriculture?s (USDA) ongoing National Food and Nutrient Analysis Program (NFNAP) to monitor the accuracy of assays of key foods and nutrients consumed in the United States. A total of 690 assigned values for individual nutrients, including proximates, vitamins, macroelements, microelements, fatty acids, amino acids, and selected phytochemicals (e.g., carotenoids), were compiled from the certificates of analysis for 63 C...

International Nuclear Information System (INIS)

The effect on human health of using food packaging made from recycled materials will depend on whether the elements will migrate into the food itself and at which levels that would be of concern. There is no EU directive for paper and board packaging, but guidelines on paper and board intended to come into contact with food are being developed in another forum. These do not propose limits for paper intended to come into contact with dry foods. However, for other foodstuffs, restriction limits in paper of 0.002 mg/dm"2 (Cd), 0.003 mg/dm"2 (Pb), and 0.002 mg/dm"2 (Hg) have been proposed. This paper describes a radiotracer method that has been applied to the determination of migration of trace elements from paper and board into real food samples.

British Library Electronic Table of Contents (United Kingdom)

As part of the United States Department of Agricultures (USDA) National Food and Nutrient Analysis Program (NFNAP), food composition data for vitamin D in the USDA National Nutrient Database for Standard Reference are being updated and expanded, focusing on high priority foods and validated analytical methodology. A lack of certified reference materials and analytical methods validated for these key foods required the development of five matrix-specific control composite materials (CC) (canned salmon and vitamin D3 fortified cereal, orange juice, milk, and cheese). Each of six experienced laboratories (research and commercial) analyzed vitamin D3 in five subsamples of each CC in five separate analytical batches, with one subsample of each material in each run. Research laboratories perform...

Energy Technology Data Exchange (ETDEWEB)

Carbohydrate and nucleotide structural determination using modern spectroscopic techniques is dependent on our ability to label oligonucleotides and oligosaccharides with stable isotopes. Uniform Carbon 13 and Nitrogen 15 labeling of oligonucleotides is important to present-day efforts, which are focused on determining the structure of relatively small oligosaccharides and oligonucleotides, which form the elements of larger structures. Because of the relatively recent interest in three-dimensional structure, the development of techniques used to label them has lagged behind parallel techniques used to label peptides and proteins. Therefore, this group`s discussion focused primarily on problems faced today in obtaining oligonucleotides labeled uniformly with carbon 13 and nitrogen 15.

Energy Technology Data Exchange (ETDEWEB)

Intact rat fat cell exposed to 12.5 ..mu..M (..gamma..-32P)ATP incorporate label into specific proteins within minutes. By solubilizing the reaction mixture with SDS which bypasses the subcellular fractionation steps, the labeled proteins can be identified in autoradiographs of SDS-PAGE gels. The most prominently labeled protein has an M/sub r/ of 42,000. Localization of this component to the cell surface can be made on the basis of inhibition of phosphorylation by addition of a protein derived from the rat brain with protein kinase inhibitory property, susceptibility of the phosphorylated protein to the tryptic digestion, inhibition of phosphorylation of this protein after brief exposure to melittin. To rule out the possibility that the cell surface protein might be a mitochondrial contaminant from broken cells, /sup 32/Pi-labeled and (..gamma..-/sup 32/P)ATP-labeled cells were ...

Science.gov (United States)

Attention deficit hyperactivity disorder (ADHD) is one of the most common behavioral disorders in children. Symptoms of ADHD include hyperactivity, low frustration tolerance, impulsivity, and inattention. While the biological pathways leading to ADHD are not clearly delineated, a number of genetic and environmental risk factors for the disorder are recognized. In the early 1970s, research conducted by Dr. Benjamin Feingold found that when hyperactive children were given a diet free of artificial food additives and dyes, symptoms of hyperactivity were reduced. While some clinical studies supported these findings, more rigorous empirical studies conducted over the next 20 years were less positive. As a result, research on the role of food additives in contributing to ADHD waned. In recent years, however, interest in this area has revived. In response to more recent research and public petitions, in December 2009 the British government requested ...

Wastenet

... (restricted)] 251-260 Revisiting new variant famine: the case of Swaziland by Scott Naysmith & Alex Waal & Alan Whiteside [Downloadable! (restricted)] 261-269 Food prices and the HIV response: findings from rapid regional assessments in eastern and southern Africa in 2008[InlineMediaObject not available: see fulltext.][InlineMediaObject not available: see fulltext.] by Stuart Gillespie & Paul Jere & John Msuya & Scott Drimie [Downloadable! (restricted)] 271-289 Declining global per capita agricultural production and warming oceans ...

Science.gov (United States)

... The revised work plan emphasized the research priorities of preharvest rodent damage to rice, the importance of postharvest food losses to rodents ...

Science.gov (United States)

from a broad range of sources including industrial and consumer products, food additives and drugs that never made it to the market to evaluate the predictive toxicity...

UK PubMed Central (United Kingdom)

Phytoestrogens are plant derived compounds found in a wide variety of foods, most notably soy. A litany of health benefits including a lowered risk of osteoporosis, heart disease, breast cancer,...Full Text Available

Science.gov (United States)

Yellow cake with chocolate frosting. Dairy: Ice cream, chocolate. Ice cream, strawberry. Ice cream, vanilla. Yogurt, frozen ...

Science.gov (United States)

cellulose casing material made by Food Products Division,. Union Carbide Corporation. tion of the anode from oxygen generated at the charging electrode. ...

Medline Plus

Enter Search terms A-Z Index Home Food Drugs Medical Devices Vaccines, Blood & Biologics Animal & Veterinary Cosmetics Radiation-Emitting Products Tobacco ...

British Library Electronic Table of Contents (United Kingdom)

Several recent studies have documented that non-human primates can individuate objects according to property and/or kind information in much the same way as human infants do from around one year of age when they begin to acquire language. Some studies suggest, however, that only some properties are used for the individuation of food items: color, but not shape. The present study investigated whether these findings reveal a true competence problem with shape properties in the food domain or whether they merely reveal a performance problem (e.g., lack of attention to shapes). We tested 25 great apes (chimpanzees, bonobos and gorillas) in two food individuation tasks. We manipulated subjects? experience with differences in color and shape properties of food items. Results indicated (i) that a...

British Library Electronic Table of Contents (United Kingdom)

Pork identification in four types of food products, which are sausages and the casings, bread and biscuits, using species-specific polymerase chain reaction (PCR) detection of a conserved region in the mitochondrial (mt) 12S ribosomal RNA (rRNA) gene was developed. Genomic DNA of the food products were successfully extracted except for the casing samples, where no genomic DNA was detected. The extracted genomic DNA was then subjected to PCR amplification targeting the specific regions of the 12S rRNA gene. The genomic DNA from the food products were found to be of good quality and produced clear PCR products on the amplification of 12S rRNA gene of 387 base pairs (bp) from pork species. The species-specific PCR identification yielded excellent results for identification of pork derivatives...

British Library Electronic Table of Contents (United Kingdom)

Roux-en-Y gastric bypass surgery (RYGB) remains to be the most effective long-term treatment for obesity and its associated comorbidities, but the specific mechanisms involved remain elusive. Because RYGB patients appear to no longer be preoccupied with thoughts about food and are satisfied with much smaller meals and calorically dilute foods, brain reward mechanisms could be involved. Just as obesity can produce maladaptive alterations in reward functions, reversal of obesity by RYGB could normalize these changes or even further reset the food reward system through changes in gut hormone secretion, aversive conditioning and/or secondary effects of weight loss. Future studies with longitudinal assessments of reward behaviors and their underlying neural circuits before and after surgery wil...

UK PubMed Central (United Kingdom)

Fava beans (Vicia fava) are cultivated rather widely in most countries of the Eastern Mediterranean area and provide a cheap but protein-rich food that can be eaten alone, in various...Full Text Available

British Library Electronic Table of Contents (United Kingdom)

The concept of antimicrobial packaging has received great attention because of its potential to enhance food safety. Several studies have explored its applications and effectiveness to suppress pathogenic microorganisms. However, few studies have analyzed the alterations caused in the engineering properties of food-packaging polymers after the incorporation of antimicrobials. Such information is very important to understand the feasibility of producing antimicrobial packaging films on the industrial scale. This review explores the work done so far to evaluate how the incorporation of antimicrobial substances affects the properties of food-packaging systems. This article also emphasizes diffusion studies on antimicrobial substances through packaging films and the analytical solutions used t...

DEFF Research Database (Denmark)

Executive summary 1. Few studies have to date explained consumer attitudes and purchase decisions with regard to genetically engineered food products. However, the increased marketing of genetically engineered food products and the considerable concern that consumers seem to express with regard to the technology call for the development of a theoretical basis for research into these issues. 2. The aim of the paper is to present three models which we have developed to explain consumer attitudes, buying behaviour and attitude change with regard to genetically engineered food products. All three models build on established consumer behaviour theory and on existing and comparable research in the field. 3. Consumer attitudes toward genetic engineering in food products are explained in an attitude model that builds on Fishbein's multiattribute attitude model. The model deviates from Fishbein's model in a ...

British Library Electronic Table of Contents (United Kingdom)

Human impacts such as eutrophication, overexploitation and climate change currently threaten future global food and drinking water supplies. Consequently, it is important that we understand how anthropogenic resource (bottom-up) and consumer (top-down) manipulations affect aquatic food web structure and production. Future climate changes are predicted to increase the inputs of terrestrial dissolved organic carbon to lakes. These carbon subsidies can either increase or decrease total basal production in aquatic food webs, depending on bacterial competition with phytoplankton for nutrients. This study examines the effects of carbon subsidies (bottom-up) on a pelagic community exposed to different levels of top-down predation. We conducted a large scale mesocosm experiment in an oligotrophic ...

UK PubMed Central (United Kingdom)

By the turn of the century, American farmers and ranchers will be producing food and fiber through the application of highly sophisticated systems that involve a broad spectrum of relevant factors--from...Full Text Available

International Nuclear Information System (INIS)

A photoaffinity derivative of the local anesthetic trimethisoquin, 5-azido-["3H]trimethisoquin (5-A["3H]T) labelled bands of app. mol. wt 50 k and 66 k. To explain the rather paradoxical labelling by 5-A["3H]T of two polypeptide chains instead of one, three possibilities were considered: (i) The site for non-competititve blockers is not carried by the 50 k or 66 k chain; however, these chains lie in the vicinity of the binding site and become preferentially labelled since they present chemical groups with which the nitrene group of 5-A["3H]T reacts. (ii) The 50 k and 66 k chains are different but carry binding sites for non-competitive blockers with similar reactivities. (iii) The 50 k chain labelled by 5-A["3H]T derives from the 66 k chain by proteolysis. These results show that the third alternative is the correct one. Under conditions which limit proteolysis, 5-A["3H]T selectively ...

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Four /sup 125/I labeled mono- and diamines were prepared and evaluated as potential brain-imaging agents. The diamines are analogues of the previously reported /sup 75/Se labeled diamines, which show high brain uptake and retention. All of the radioiodinated amines display high initial brain uptake in rats after intravenous injection (1.7-2.4% dose/organ). The xylylenediamines show prolonged brain retention (t1/2 approximately 18 h), which is desirable for brain imaging. In contrast, the benzylamine is rapidly cleared from brain tissue (t1/2 approximately 15 min).

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Photosynthetic carbohydrates from the leaves are exported through the phloem to growing tips, roots, flowers and fruits. If sucrose labelled with {sup 14}C is applied to the leaves of bean plants, the pathway for sugar movement may be readily observed by autoradiography. Students apply the labelled sucrose during class time and return the next day to press their plants. During the next class, the pressed and dried plants are placed against X-ray film and left in the dark for four weeks. the film is then developed, examined for presence of label and compared to the pressed plants. Source to sink movement is clearly illustrated and information about the mechanism of phloem transport and loading is gained through experimental treatments, which include blocking the phloem pathway and inhibiting energy production.

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Russian (1972). USSR Prokof'eva, MS (no.188) p. 217-222. age

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... ammonium compounds antigen-antibody reactions hemoglobin inhibition

International Nuclear Information System (INIS)

English 1999 p. 47 Brazil Oliveira, JF Braga, ACS Avila, ASR Bernardo-Filho,

UK PubMed Central (United Kingdom)

The binding of iodine-labelled plasminogen to Helicobacter pylori CCUG 17874 was characterized. Inhibition of the binding was observed after preincubation of H. pylori...Full Text Available

International Nuclear Information System (INIS)

1981. p. 21. France Ghanem, G. Roland, MT Libert, A. Gossuin, A. Maerschalk,

International Nuclear Information System (INIS)

The human erythrocyte (RBC) Rh blood group system consists of a complex of distinct integral membrane polypeptides with physical properties common to the aminophospholipid transporter responsible for the transbilayer movement of phosphatidylserine (PS) in RBC. To assess the involvement of Rh polypeptides in PS translocation, the aminophospholipid translocase was labeled with a photoactivatable PS analogue, "1"2"5I-azido-PS, and with an inhibitor of PS transport, "1"2"5I-labeled 2-(2-pyridyldithio)ethylamine. The ability of monoclonal Rh antibodies to immunoprecipitate the labeled transporter was determined. Immunoprecipitated Rh polypeptides were found to be labeled with the aminophospholipid translocase markers, suggesting that Rh proteins are involved in the transbilayer movement of PS.

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Portuguese 1999 p. 18 Brazil Oliveira, JF Braga, ACS Bezerra, RJAC Bernardo

International Nuclear Information System (INIS)

Additional measurements have been made of deposition and retention of inhaled radioactively labeled iron oxide aerosols generated by a spinning top aerosol generator.

Science.gov (United States)

Advanced Solid Tumors With and Without Brain Metastases

British Library Electronic Table of Contents (United Kingdom)

The ?new variant famine? hypothesis posits links between HIV/AIDS and new patterns of impoverishment, food insecurity and hunger, in southern and eastern Africa. This paper explores the relevance of the NVF hypothesis to understanding Swaziland?s recurrent food crises and high HIV prevalence. Evidence exists that all four markers of NVF are present in Swaziland. The national government and the international community will have to contend with this phenomenon in future planning for the wellbeing of Swazi citizens.

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The K 2010 exhibition saw the introduction of PolyOne Corps OnCap Antifog Additive for polypropylene (PP) films, the first such additive for PP films that does not require corona treatment. According to the company, this new additive simplifies production of antifog films for the flexible food packaging industry, improving production efficiencies for both cast and blown film producers.

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Total mercury (THg), methylmercury (MeHg) and 22 other trace elements were measured in ice algae, three species of zooplankton, mixed zooplankton samples, Arctic cod (Boreogadus saida), ringed seals (Phoca hispida) and eight species of seabirds to examine the trophodynamics of these metals in an Arctic marine food web. All samples were collected in 1998 in the Northwater Polynya (NOW) located between Ellesmere Island and Greenland in Baffin Bay. THg and MeHg were found to biomagnify through the NOW food web, based on significant positive relationships between log THg and log MeHg concentrations vs. {delta} {sup 15}N muscle and liver . The slope of these relationships for muscle THg and MeHg concentrations (slope = 0.197 and 0.223, respectively) were similar to those reported for other aquatic food webs. The food web behavior of THg and {delta} {sup 15}N appears constant, regardless of trophic state ...

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...six months Organising local tours of the area for up to 1000 affected families in the Izu Penninsula area around Mount Fuji Mobile food stalls that wlll serve up to 10,000 bottles of water and 10,000 servings of food Mobile human waste disposal units Building northern Japan support committees Browser does not support script. Browser does not support script. For further information, please contact: Garry Campbell, PR & ...

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...] Responsibility and the Big Society by Antje Bednarek [Downloadable!] Friendship Dynamics Between Emotions and Trials by Paola Rebughini [Downloadable!] On Friendship, Equality and Introductions: Comparing English and German Regimes of Manners and Emotions by Cas Wouters [Downloadable!] Fees, Funding and Overseas Study: Mobile UK Students and Educational Inequalities by Rachel Brooks & Johanna Waters [Downloadable!] The Subject of Functional Foods: Accounts of Using Foods Containing Phytosterols by Kate Weiner [Downloadable!] Paradoxes and Pitfalls in ...

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The gastrointestinal absorption of lead was markedly increased in human subjects who had fasted before ingesting lead. Food in the gut was found to reduce lead absorption, and the effect of dietary constituents on /sup 203/Pb absorption was investigated. The greatest reduction in absorption was caused by minerals. This dependence of lead absorption on dietary constituents complicates the establishment of safe levels of lead in food and water.

Environmental Research Database

DescriptionThe objective is to exploit the use of wheat as an industrial raw material and understand the effect of material variation in wheat starch and flour on final material properties for food packaging applications. The project will focus on property enhancement in terms of stiffness, strength, toughness, moisture barrier resistance, transparency, ageing resistance, microbial stability and biodegradability of starch-based packaging materials made by sheet extrusion and thermoforming processes, in ord [continued...

British Library Electronic Table of Contents (United Kingdom)

The purpose of this study was to determine if a cannabinoid CB1 receptor antagonist would selectively decrease consumption of highly palatable food in non-human primates. The CB1 receptor antagonist SR141716 (rimonabant; 0.12?1.0?mg/kg, i.m.) and the stimulant anorectic drug d-amphetamine (0.12?1.0?mg/kg, i.m.) were administered to non-food deprived baboons for the purpose of measuring the effect of each drug on consumption of the normal diet, and a large single meal of a high-carbohydrate candy. Four male and four female baboons had access to food 24?h each day, but they had to complete a two phase operant procedure in order to eat. Responding on one lever during a 30-min appetitive phase was required before animals could start a consumption phase, where responding on another lever led to...

UK PubMed Central (United Kingdom)

The effect of abatacept, a selective T-cell co-stimulation modulator, on vaccination has not been previously investigated. In this open-label, single-dose, randomized, parallel-group, controlled study,...Full Text Available

UK PubMed Central (United Kingdom)

The modes of action of the five major endo-(1----4)-beta-D-glucanases (I, II, III, IV and V) purified from Penicillium pinophilum cellulase were compared by h.p.l.c. analysis, with normal, 1-3H-labelled...Full Text Available

UK PubMed Central (United Kingdom)

We present evidence that the role of tryptophan and other potential intermediates in the pathways that could lead to indole derivatives needs to be reexamined. Two lines of Lemna gibba...Full Text Available

UK PubMed Central (United Kingdom)

The rate of appearance of labeled thyroxine (T4) and albumin in lymph from various areas after simultaneous i.v. injection of the labeled substances in conscious ambulatory sheep has been used to estimate...Full Text Available

International Nuclear Information System (INIS)

... affinity azido compounds biosynthesis dopamine iodine compounds iodine

International Nuclear Information System (INIS)

HIPDM is an "1"2"3I-labeled agent with a distribution in brain reflecting regional perfusion. This compound is neutral and lipid soluble at blood pH and freely crosses the blood-brain barrier. At the lower pH in brain, it picks up a hydrogen ion and becomes positively charged. In this form the molecule is not lipid soluble and it is trapped in brain.

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The obtained results in presently study demonstrate that the feasibility of the detection of the sentry ganglion in cervix uterine cancer using a boarding by laparoscopic via, being necessary the use of twice labelled as much with patent blue and radioisotope (colloid of labelled rhenium with "9"9"mTc, total dose of 3 MCi) to achieve the identification of the ganglion. (Author)

UK PubMed Central (United Kingdom)

Radioimmunoassays for detecting cell-associated or released virus are described using either ¹²⁵I- or [³H]acetate-labeled antibodies. In the first assay system, antigen-antibody...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

Research is described in the development of organometallic reagents in which the boron was attached to a nonreactive organic or inorganic matrix such as polystyrene, silica, or alumina. We developed the synthesis of oxygen-15 labelled butanol, which has been found to be a valuable blood flow agent in humans. We have also developed a series of polymeric borane derivatives which were used to prepare nitrogen-13 labelled amines.

International Nuclear Information System (INIS)

Ribosomal protein L 10 from the livers of trout, hen, and rat was found to be the main target for 2-nitro-4-azidobenzoyl-Phe-tRNA in affinity labelling experiments. Therefore, despite somewhat different electrophoretic mobilities, this protein seems to be involved in the organization of the peptidyl transferase centre in ribosomes of various vertebrates. (author).

Energy Technology Data Exchange (ETDEWEB)

Cell extracts of the thermophile Clostridium thermohydrosulfuricum catalyzed the phosphorylation by (..gamma..-/sup 32/P)ATP of several endogenous proteins with M/sub r/s between 13,000 and 100,000. Serine and tyrosine were the main acceptors. Distinct substrate proteins were found in the soluble (e.g., proteins p66, p63, and p53 of M/sub r/s 66,000, 63,000, and 53,000, respectively) and particulate (p76 and p30) fractions, both of which contained protein kinase and phosphatase activity. The soluble fraction suppressed the phosphorylation of particulate proteins and contained a protein kinase inhibitor. Phosphorylation of p53 was promoted by 10..mu..M fructose 1,6-bisphosphate or glucose 1,6-bisphosphate and suppressed by hexose monophosphates, whereas p30 and p13 were suppressed by 5 ..mu..M brain (but not spinach) calmodulin. Polyamines, including the odd polyamines characteristic of thermophiles, modulated the labeling of most of the phosphoproteins. Apart from ...

International Nuclear Information System (INIS)

The heavy chains of Acanthamoeba myosins, IA, IB and II, turkey gizzard myosin, and rabbit skeletal muscle myosin subfragment-1 were specifically labeled by radioactive ATP, ADP, and UTP, each of which is a substrate or product of myosin ATPase activity, when irradiated with uv light at 0"0C. With UTP, as much as 0.45 mol/mol of Acanthamoeba myosin IA heavy chain and 1 mol/mol of turkey gizzard myosin heavy chain was incorporated. Evidence that the ligands were associated with the catalytic site included the observations that reaction occurred only with nucleotides that are substrates or products of the ATPase activity; that the reaction was blocked by pyrophosphate which is an inhibitor of the ATPase activity; that ATP was bound as ADP; and that label was probably restricted to a single peptide following limited subtilisin proteolysis of labeled Acanthamoeba myosin IA heavy chain and extensive cleavage with CNBr and ...

International Nuclear Information System (INIS)

Energy expenditure (EE) of four adult men on a weight-maintenance diet was estimated by use of doubly labeled water, intake balance, and direct and indirect calorimetry. The doubly labeled water (2H218O) method was used to estimate free-living EE for 13 d. Metabolizable energy (ME) intake was used to estimate free-living EE for 1 wk. The subjects' 24-h EE was measured in a dual direct-indirect room calorimeter on 3 alternate days. Estimates of free-living EE as measured by ME intake and doubly labeled water indicate agreement between the two methods (mean difference +/- SEM, -1.04 +/- 0.63%). Measurements of EE with indirect and direct calorimetry are equivalent (mean difference 0.63 +/- 0.44%). The daily EE measured by doubly labeled water in these free-living adults over a 13-d period was 15.01% greater than the 24-h EE measured within the calorimeter.

International Nuclear Information System (INIS)

The main aim of any food irradiation treatment is to guarantee the best safe quality of the products, reducing the spreading risk ("cross-contamination") for several food-associated diseases. Actually, over 40 countries provide clearances for the treatment of about 45 different types of foodstuffs. EU has to homogenise the situation within the associated States. With the European directive 1999/2/EC Italy, as other EU countries, already has brought into force their regulations to comply. The current Italian regulation on irradiation treatment of foodstuffs is referred since 1996 as follows: a) potatoes, onions and garlic; b) spices, herbs and condiments microbial. The new (April 2001) Italian law allows the possibility to ask for special permission of treatment for other foodstuff which is possible to treat in other E.U. countries. Large majority of foods are submitted to irradiation treatment after they have been packaged. ...

International Nuclear Information System (INIS)

Soils and food crops from a former tin mining location in a high background radiation area on the Jos-Plateau, Nigeria were collected and analyzed by gamma spectrometry to measure their contents of "4"0K, "2"3"8U and "2"3"2Th. As well as collecting samples, in situ dose rates on farms were measured using a precalibrated survey meter. Activity concentrations determined in food crops were compared with the local food derivatives or diets to investigate the possible removal or addition of radionuclides during food preparation by cooking or other means. Potassium-40 was found to contribute the highest activity in all the food products. The activity concentration of "4"0K, "2"3"8U and "2"3"2Th in local prepared diets ranged between 60 and 494 Bq kg"-"1, between BDL and 48 Bq kg"-"1 and between BDL and 17 Bq kg"-"1, respectively. The internal effective dose to individuals from the ...

Energy Technology Data Exchange (ETDEWEB)

The project was designed to solve the infra structural problem required for commercialization of food irradiation. In improvement of physical properties of corn starch, gamma irradiation was effective for increasing glucose productivity and for substituting traditional modified starches (acid modified starch, oxidized starch). In immobilization of microorganisms, the mass production method of natural red pigment was developed by using immobilized mold pellets. In Korean medicinal plants, 10 kGy gamma irradiation was effective for improving sanitary quality and increasing extraction yield. In evaluation of wholesomeness, gamma irradiated red ginseng could be safe on the genotoxic point of view. And also, six items of irradiated foods approved for human consumption from Korea ministry of health and welfare in May 19, 1995. 30 figs, 20 tabs, 54 refs. (Author).

British Library Electronic Table of Contents (United Kingdom)

Zooplankton tolerant to ultraviolet radiation (UVR) could be indirectly affected by UVR through interactions with UV-sensitive species in the same ecosystem. In Lake Giles, Pennsylvania, USA, the calanoid copepod Leptodiaptomus minutus is more UVR tolerant than the cohabiting species Daphnia catawba and Cyclops scutifer. We asked whether L. minutus is affected by UV-induced mortality of a food competitor (D. catawba) or a predator of its nauplii (C. scutifer). We conducted two in situ enclosure experiments with six treatments: L. minutus alone, L . minutus + Daphnia and L. minutus + Cyclops in the presence and absence of UVR. There were few differences in survival among treatments in Experiment 1, which had enhanced food and a cumulative UVR (320 nm) dose of 9.3 kJ m-2. In Experiment 2, wh...

British Library Electronic Table of Contents (United Kingdom)

Abstract In response to growing consumer concerns, developed-country governments have reduced permissible pesticide residue levels in food. Many food retailers have developed even more stringent private food safety protocols relating to pesticide use, storage and disposal and passed them on to their suppliers. Exporters in developing countries enforce these developed-country pesticide standards (DC-PS) by subjecting farmers to close monitoring. This study explores the effects of enforcing compliance with DC-PS on smallholder farmers' pesticide-related health costs. Results suggest that enforcing DC-PS encourages farmers to use protection that lowers pesticide-induced morbidity, hence reducing farmers' health costs from pesticide exposure. The study concludes that there are health benefits ...

British Library Electronic Table of Contents (United Kingdom)

The need for safe food products has motivated food scientists and industry to find novel technologies for antimicrobial delivery for improving food safety and quality. Controlled release packaging is a novel technology that uses the package to deliver antimicrobials in a controlled manner and sustain antimicrobial stress on the targeted microorganism over the required shelf life. This work studied the effect of controlled release of nisin to inhibit growth of Micrococcus luteus (a model microorganism) using a computerized syringe pump system to mimic the release of nisin from packaging films which was characterized by an initially fast rate and a slower rate as time progressed. The results show that controlled release of nisin was strikingly more effective than instantly added (?formulated...

British Library Electronic Table of Contents (United Kingdom)

Background Little is known about the role of diet in the development of venous thromboembolism (VTE). We explored the prospective relation of dietary patterns, food groups, and nutrients to incident VTE in older women. Methods In 1986, Iowa women aged 55 to 69 years completed a mailed survey, including a 127-item food frequency questionnaire. These data were linked to Medicare data from 1986 to 2004, and International Classification of Diseases discharge codes were used to identify hospitalized VTE cases. Cox regression analyses evaluated relations of 2 principal components?derived dietary patterns, 11 food groups, and 6 nutrients to VTE, adjusted for age, education, smoking status, physical activity, and energy intake. Results Over 19 years of follow-up, 1,950 of the 37,393 women develope...

British Library Electronic Table of Contents (United Kingdom)

Abstract:- Significant interest has emerged in the introduction of food packaging materials manufactured from biodegradable polymers that have the potential to reduce the environmental impacts associated with conventional packaging materials. Current technologies in active packaging enable effective antimicrobial (AM) packaging films to be prepared from biodegradable materials that have been modified and/or blended with different compatible materials and/or plasticisers. A wide range of AM films prepared from modified biodegradable materials have the potential to be used for packaging of various food products. This review examines biodegradable polymers derived from polysaccharides and protein-based materials for their potential use in packaging systems designed for the protection of food ...

International Nuclear Information System (INIS)

Insulitis is considered the histopathological hallmark of type I diabetes. In the non-obese diabetic (NOD) mouse, diabetes has never been observed in the absence of insulitis. The in vivo detection of insulitis could be of relevance for early prediction of diabetes. As approximately 15% of islet-infiltrating lymphocytes express interleukin 2 receptors, the authors have labelled recombinant inter-leukin 2 with "1"2"3I and used this radiopharmaceutical to detect insulitis by gamma camera imaging. The authors studied 71 prediabetic NOD and 27 normal Balb/c mice. Labelled #alpha#-lactalbumin was used as the control protein. In the first set of experiments the tissue distribution of radiolabelled interleukin 2 in isolated organs from animals sacrificed at different time points was studied. Higher radioactivity was detected in the pancreas of NOD mice injected with labelled interleukin 2, as compared to NOD mice receiving ...

International Nuclear Information System (INIS)

Four "1"2"5I labeled mono- and diamines were prepared and evaluated as potential brain-imaging agents. The diamines are analogues of the previously reported "7"5Se labeled diamines, which show high brain uptake and retention. All of the radioiodinated amines display high initial brain uptake in rats after intravenous injection (1.7-2.4% dose/organ). The xylylenediamines show prolonged brain retention (t1/2 approximately 18 h), which is desirable for brain imaging. In contrast, the benzylamine is rapidly cleared from brain tissue (t1/2 approximately 15 min).

International Nuclear Information System (INIS)

A brief survey is presented of the methods of preparation and of the applications of radiopharmaceuticals labelled with short-lived positron radionuclides "1"1C, "1"8F, "1"3N and "1"5O which, thanks to their energy level schemes, short half-life and the 511 keV photon radiation energy are almost ideal tracers in modern nuclear medicine and pharmacology. In conjunction with computerized tomography, they represent one of the most sensitive diagnostic imaging methods, the so-called positron emission tomography. In addition, their incorporation in the molecule of a pharmaceutical does not change the biological and chemical properties of the original molecule unlike those of radiopharmaceuticals labelled with technetium, iodine, etc. (author). 125 refs.

Energy Technology Data Exchange (ETDEWEB)

Wheat leaves can be induced by excision to produce fructans. Fructose residues of newly made oligofructans in leaves labeled in vivo with {sup 14}CO{sub 2} are not equally labeled. We report here on a fructosyl transferase activity in wheat leaves catalyzing the reaction: G{sup *}-F{sup *} + G-F-F = G{sup *}-F{sup *}-F + G-F. This activity, described previously in J. artichoke was attributed to fructan:fructan fructosyl transferase (FFT). The rate of this reaction in vitro is much higher than that of net kestose synthesis by SST. Hence, appearance of labeled 1-kestose from sucrose may not be an accurate measure of SST, but a curious reshuffling of hexoses between pools of 1-kestose and sucrose.

International Nuclear Information System (INIS)

Hydrophobic labelling is frequently used in the study of membrane-inserted domains of intrinsic proteins. However, the published procedures, fail to incorporate sufficient radioactivity into membrane immunoglobulins of B lymhocytes to permit investigation of their subunit structures and associations with other proteins. In order to increase the specific radioactivity of ["1"2"5I]iodonaphtylazide ["1"2"5I]INA), an improved method for the synthesis of the reagent was developed. In addition, the optimal conditions for labelling B lymhpocytes with ["1"2"5I]INA and commercially available reagent 3-(trifluoromethyl)-3-(trifluoromethyl)-3-(3'-["1"2"5Iliodophenyl)diazirine (["1"2"5I]TID were isolated and analysed in detail by SDS-PAGE. The usefulness of the two reagents for the investigation of lipid-embedded domains of membrane proteins is discussed. (author). 28 refs.; 4 figs.

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The iodinated oil lipiodol is commonly used as a carrier for in situ delivery of drugs or radioactivity to hepatic tumors. Recently, we reported a new kit formulation for high-activity labeling of lipiodol with the {beta}-emitting radionuclide Re-188. Since the whole preparation involves different steps and complex manipulations of high-activity samples, we describe here an automated synthesis module that allows the easy preparation of sterile and pyrogen-free samples of Re-188 lipiodol ready to be administered to the patient. Important advantages include the possibility to incorporate high Re-188 activity into the lipiodol hydrophobic phase and a sharp reduction of radiation exposure of the operator assisting the labelling procedure. Application of this modular reaction system could be also extended to the preparation of other Re-188 radiopharmaceuticals and to compound labelled with different {beta}-emitting therapeutic ...

Science.gov (United States)

of satellite, aircraft and ground-based observations. In ..... swarm traps by Utah Department of Food and Agriculture (DAF) personnel. Preliminary Results ..... the 150th anniversary of the book's publication and the 200th anniversary of Darwin's birth. ...... cold war submarine missions to find that Arctic Ocean ...

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1 yellow cake mix 1 container star sprinkles 1 box of regular ice cream cones 2 vanilla frosting containers 1 box Teddy Grahams Green food coloring ...

Science.gov (United States)

Skip to Content Cancer Control and Population Sciences Home Applied Research Home Risk Factor Monitoring and Methods Home Diet Usual Dietary Intakes: Background The NCI Method Details of the NCI Method Food Intakes, US Population, 2001-04 Selected Intakes

UK PubMed Central (United Kingdom)

This report describes a case of toxic myopathy in a two year old sheltie dog with clinical signs of profound weakness, myoglobinuria, and muscle enzyme elevations. The clinical signs were likely related...Full Text Available

UK PubMed Central (United Kingdom)

The ability to enter a hypometabolic state upon restriction of caloric intake is pivotal for animal survival: balancing the energy budget in endotherms can be a real struggle when food is not...Full Text Available

Science.gov (United States)

Issues Democracy and Human Rights Economic Issues Energy Security Food Security Haiti Health Iran Iraq Landmines Middle East Muslim Outreach Narcotics Nonproliferation North Korea...

UK PubMed Central (United Kingdom)

Objectives:The supermarket industry now services many customers through online food shopping over the Internet. The Internet shopping process offers a novel opportunity...Full Text Available

UK PubMed Central (United Kingdom)

Allergic rhinitis is common among children and quite often represents a stage of the atopic march. Although sensitization to food and airborne allergens may appear in infancy and early childhood, symptoms...Full Text Available

UK PubMed Central (United Kingdom)

Separate groups of rats received 500 trials of lever-press training under autoshaping (food delivery followed 10-second lever presentations, or occurred immediately following a response); operant conditioning...Full Text Available

UK PubMed Central (United Kingdom)

This unit presents two basic protocols that offer rapid assessments of anosmia (the absence of a sense of smell) in mice. The buried food test is used to check for the ability to smell volatile...Full Text Available

UK PubMed Central (United Kingdom)

SummaryBackgroundThe US FDA has been collecting information on medical devices involved in significant adverse advents since 1984. These reports have...Full Text Available

UK PubMed Central (United Kingdom)

IntroductionRecent Food and Drug Administration legislation enables the mandating of product performance standards for cigarette smoke and the evaluation of manufacturers’...Full Text Available

UK PubMed Central (United Kingdom)

Mouse models for the study of autistic-like behaviors are increasingly needed to test hypotheses about the causes of autism, and to evaluate potential treatments. Both the automated 3-chambered...Full Text Available

UK PubMed Central (United Kingdom)

Chemoprevention, especially through the use of naturally occurring phytochemicals capable of impeding the process of carcinogenesis at one or more steps, is an ideal approach for cancer management....Full Text Available

UK PubMed Central (United Kingdom)

In this study, our main objective was to determine whether energy restriction (ER) affects the rate of oxygen consumption of mice transiently or lastingly and whether metabolic rate plays a role in...Full Text Available

UK PubMed Central (United Kingdom)

Sensory analysis studies are critical in the development of quality enhanced crops, and may be an important component in the public acceptance of genetically modified foods. It has recently been established...Full Text Available

UK PubMed Central (United Kingdom)

The importance of a properly functioning and well-balanced immune system for maintaining health has become strikingly evident over the past decades. Roughly since World War II, there has been an apparent...Full Text Available

Environmental Research Database

ObjectivesThis working group aims to conduct an in-depth analysis and synthesis of the health risks and benefits of producing and consuming your own food in urban areas. It will have a particular focus on urban soil pollutants - including both toxic elements and persistent organic pollutants that can pass up through the food chain or be ingested in soil. It will consider pathways of pollutant transport and deposition and human uptake from urban cultivation. Against the health risks associated with expos [continued...]DescriptionIn the UK there are over 250,000 allotment holders, many in urban areas, and in many city gardens fruit and vegetables are grown, often in regions known to have a legacy of environmental pollution. The activities of cultivating and eating 'home grown' foods holds both risks and benefits, yet the balance of risk and benefits and the resulting net implications for human health have not been clearly ...

UK PubMed Central (United Kingdom)

Fungi have long been known to affect human well being in various ways, including disease of essential crop plants, decay of stored foods with possible concomitant production of mycotoxins, superficial...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundObesity has reached epidemic proportions in the United States. It is implicated in the development of a variety of chronic disease states and is associated with increased...Full Text Available

UK PubMed Central (United Kingdom)

Spatial abilities have been associated with many ecologically-relevant behaviors such as territoriality, mate choice, navigation and acquisition of food resources. Differential demands on spatial...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundDiet is considered an important factor for bone health, but is composed of a wide variety of foods containing complex combinations of nutrients. Therefore we investigated...Full Text Available

UK PubMed Central (United Kingdom)

Food-deprived pigeons were presented with a row of four response keys situated above a grain hopper aperture. At the start of a trial, three of four keys were randomly selected and illuminated white...Full Text Available

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...options box during checkout as this item typically ships in its own packaging. 3 new from $29.95 Your Favorite Coffee, ...

UK PubMed Central (United Kingdom)

Current pesticide health risk assessments in the United States require the characterization of aggregate exposure and cumulative risk in the setting of food tolerances. Biologic monitoring can aggregate...Full Text Available

International Nuclear Information System (INIS)

Described and discussed in this paper are radiation processes and their advantages over the conventional techniques. Radiation sterilization of medical products, food irradiation, wood plastic composites, and radiation treatment of sewage and waste waters are presented. The Philippine experience in using these technologies, its problems and barriers are also given. (ELC).

UK PubMed Central (United Kingdom)

With the increase in multi-cultural families, Korea is seeing a rapid increase in immigrated housewives, who are closely related to food culture. However, studies for the diet of multi-cultural families,...Full Text Available

UK PubMed Central (United Kingdom)

A simplification of Sandell's method for estimating nickel in urine is described. Nickel is a normal constituent of most articles of food and between 0·01 and 0·03 p.p.m. are found in...Full Text Available

Energy Technology Data Exchange (ETDEWEB)

Some crops of important nutritive requirements have been collected from farmlands located in Bitsichi an old tin mining town in north central region of Nigeria. The food crops were analyzed in order to determine trace element concentrations level using Neutron Activation Analysis (NAA). The activity concentrations due to natural radionuclides in the food samples and soil samples collected within the root zone of the crops were also determined using gamma-ray spectrometry (GRS). The NAA results obtained showed higher concentration of Potassium among the essential elements in all the crops. Calcium concentration in both maize and Guinea corn was below detection limit and also Zn in Green beans, sweet potato and cassava. The trace elements; Sc, Hf, Sm, and Th, were below detection limit in all the crops except in Green beans. Rubidium (Rb) and lanthanum (La) were below detection limits only in Cabbage. The activity concentrations of the natural ...

Energy Technology Data Exchange (ETDEWEB)

This conference provided a forum to discuss advances in the application of bioengineering principles and practices dealing with biological systems for the production of food, bioproducts and energy. The topics of discussion ranged from engineering practices in agriculture, forestry, bioresources, biochemistry and biosystems. The sessions of the conference were entitled: renewable energy; waste management; soil and water; machinery systems; building and storage systems; and bioproducts and food. The conference also featured a workshop on integrated biomass supply and logistics. In addition to 20 posters, the conference featured 67 presentations, of which 3 have been catalogued separately for inclusion in this database. refs., tabs., figs.

British Library Electronic Table of Contents (United Kingdom)

Malnutrition?in the form of insufficient energy intakes?affects millions of people worldwide and the negative impact of this kind of hunger is well acknowledged, not least by agronomists trying to increase yields to ensure a sufficient supply of food. This review focuses on another, more particular and ?hidden? form of malnutrition, namely mineral malnutrition. It illustrates the burden of disease that is caused by mineral deficiencies and the social and economic consequences they bring about. Mineral malnutrition has a considerable negative impact on individual well-being, social welfare and economic productivity. Agricultural scientists should keep the nutritional qualities of food in mind and?next to optimizing the agricultural properties of crops that are paramount for their adoption b...

Science.gov (United States)

An abundance of waste corn, a key food of many wildlife species, has helped make possible the widespread success of wildlife management in the United States over the past half century. We found waste corn post harvest in Nebraska declined by 47% from 1978 to 1998 due primarily to improvements in combine headers resulting in a marked decline in ear loss. The reduction in waste coincided with major declines in fat storage by sandhill cranes and white-fronted geese during spring migration. Sandhill cranes, northern pintails, white-fronted geese, and lesser snow geese avoided soybeans while staging in spring in the Rainwater Basin Area and Central Platte River Valley. These findings and other literature suggest soybeans are a marginal food for wildlife particularly during periods of high energy requirements. Soybean acreage has increased by 600% in the United States since 1950 and now comprises nearly one-quarter of the nation>'s cropland. With ...

UK PubMed Central (United Kingdom)

A commercially available synthetic nucleic acid probe (SNAP) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting Campylobacter species. The SNAP was able to...Full Text Available

International Nuclear Information System (INIS)

Using nitrogen-13 initial measurements have been made of rumen ammonia-nitrogen pool size and turnover, and rate of incorporation of ammonia into microbial protein. It is also feasible to measure the rate of incorporation of absorbed ammonia into urea and the appearance of label in saliva.

UK PubMed Central (United Kingdom)

The metabolism of ¹⁴C-labeled fatty acids and triacylglycerols was followed in intact germinating oil palm seedlings as well as in tissue slices. In the germinating seedling, the shoot contained...Full Text Available

International Nuclear Information System (INIS)

... Technology; Warsaw (Poland) 978-83-909690-8-4 221 p. RADIOLOGY AND

UK PubMed Central (United Kingdom)

By pretreating simian virus 40-infected BSC-1 cells with glucosamine, [³H]uridine labeling of both cellular and viral RNA can be halted instantaneously by addition of cold uridine. We have...Full Text Available

UK PubMed Central (United Kingdom)

The adenovirus fiber knob causes the first step in the interaction of adenovirus with cell membrane receptors. To obtain information on the receptor binding site(s), the interaction of labeled cell...Full Text Available

International Nuclear Information System (INIS)

Platelet deposition on bovine pericardial-tissue mitral-valve prostheses in 11 dogs was observed noninvasively by use of "1"1"1In-labeled platelets and quantified after sacrifice at one (n . 3), 14 (n . 3), and 30 (n . 5) days postimplantation (300-400 microCi of labeled platelets having been injected 24 hours previously). Thrombosis on the sewing ring and pericardial leaflets at one and 14 days and on the leaflets at 30 days was delineated in scintiphotos. In vitro quantification (% injected dose) indicated that the leaflets, sewing ring, and perivalvular tissue retained 0.904% of labeled platelets at one day postimplantation, 0.198% at 14 days, and 0.040% at 30 days. Platelet half-life was reduced to 38 hours at 21 days postimplantation but returned toward the normal (50 hours) with fibrous ingrowth in the sewing ring. Microembolism in lung and kidney, as measured by tissue/blood radioactivity ratio, also was decreased ...

UK PubMed Central (United Kingdom)

Skin sensitization is the most commonly reported occupational illness, causing much suffering to a wide range of people. Identification and labeling of environmental allergens is urgently required to...Full Text Available

UK PubMed Central (United Kingdom)

BackgroundZibotentan (ZD4054) is a specific endothelin A (ET_A) receptor antagonist being investigated for the treatment of prostate cancer. As zibotentan is eliminated...Full Text Available

UK PubMed Central (United Kingdom)

To gain insight into the process of mitochondrial transmission in yeast, we directly labeled mitochondrial proteins and mitochondrial DNA (mtDNA) and observed their fate after the fusion of two cells....Full Text Available

UK PubMed Central (United Kingdom)

The somatic sensory system responds to stimuli of distinct modalities, including touch, pain, itch, and temperature sensitivity. In the past century, great progress has been made in understanding the...Full Text Available

UK PubMed Central (United Kingdom)

To investigate the fate of intrinsic factor and cobalamin during cobalamin absorption, we incubated enterocytes isolated from guinea pig ileum for periods of up to 30 min with ⁵⁷Co-labeled...Full Text Available

International Nuclear Information System (INIS)

Portuguese 2006 1 p. Brazil Rebello, BM Florentino, DM Godinho, CR

Energy Technology Data Exchange (ETDEWEB)

The sucrose derivative 6'-deoxy-6'-(2-hydroxy-4-azido)benzamidosucrose (6'-HABS) was prepared from sucrose (via 6'-deoxy-6'-aminosucrose) and 4-amino-salicylic acid. 6'-HABS is a competitive inhibitor of sucrose influx into protoplasts from developing soybean cotyledons and of sucrose binding to membranes from the bacteria P. saccharophila. The Ki for inhibition in the soybean protoplasts was 75..mu..M. 6'-Deoxy-6'-(2-hydroxy-3-/sup 125/Iodo-4-azido)benzamidosucrose was prepared by lactoperoxidase iodination of 6'-HABS. Upon photolysis in the presence of membranes from P saccharophila, label from the photoprobe is incorporated into a sucrose inducible polypeptide of mass 84 KD in SDS-PAGE. The polypeptide is protected from labeling by the inclusion of sucrose in the photolysis mixture. Photolysis conditions which lead to specific labeling of ...

International Nuclear Information System (INIS)

This paper describes the evaluation of antibody coated polystyrene beads using "1"2"5I-labelled T_3 ("1"2"5I-T_3). (author).

Energy Technology Data Exchange (ETDEWEB)

No abstract prepared.

UK PubMed Central (United Kingdom)

Colonies of toxin-producing Pasteurella multocida were detected with peroxidase-labeled monoclonal antibodies by a membrane assay. Examination of the specificity of the assay with 29 P. multocida cultures...Full Text Available

UK PubMed Central (United Kingdom)

A solid-phase radioimmunoassay employing ¹²⁵I-labeled enterotoxins and polystyrene tubes coated with specific antibody has been developed for assaying the relative concentrations of antibodies...Full Text Available

UK PubMed Central (United Kingdom)

This paper reviews the development of cerebral scintigraphy from its early days of planar imaging with simple technetium-99m labelled compounds to the recent revival of the technique in the form of...Full Text Available

UK PubMed Central (United Kingdom)

Hairpin oligonucleotides were synthesized with stems ending in a double-stranded structure, which can be ligated to double-strand breaks in DNA, and with loops that contain nucleotides modified by the...Full Text Available

UK PubMed Central (United Kingdom)

Amino acids labelled with dimethylaminoazobenzenesulphonyl chloride can be separated by reversed-phase high-pressure liquid chromatography and detected in the visible region (436 nm). All 19 naturally...Full Text Available

Science.gov (United States)

Syntheses of all five of the singly labeled (/sup 15/N)adenines are now provided. The presence or absence of two-bond /sup 15/N-/sup 1/H spin couplings in their /sup 1/H NMR spectra confirm the location of the isotope in each case. The fragmentation patterns in their mass spectra are indicative of the sequential losses of HCN units and of CH/sub 2/N/sub 2/ from adenine upon electron impact.

Energy Technology Data Exchange (ETDEWEB)

An isotopic exchange method was used to label lipiodol with [sup 131]I. The labelling efficiency was > 92.5%, and the radiochemical purity of [[sup 131]I]lipiodol was above 98% as determined by ITLC. The influencing factors e.g. the heating temperature, reaction, pH and storage conditions were studied and the optimum conditions were determined. In a pilot study injecting [[sup 131]I]lipiodol for the treatment of hepatoma, about 70% of hepatoma patients had a response to the treatment with a reduction of [alpha]-fetoprotein and decrease of hepatoma sizes. The overall median survival was 9 months (range 2-17 months). (author).

Energy Technology Data Exchange (ETDEWEB)

An azido-[sup 125]iodo-ansamycin (1) in the geldanamycin family was prepared in two steps in one reaction vessel from 17-amino-22-(4'-aminophenacyl)-17-demethoxygeldanamycin (3). The title compound was suitable for photoaffinity labeling proteins that interacted with ansamycins. An alternative synthesis is reported for preparation of unlabeled title compound in order to afford practical amounts of (1) for conventional biochemical studies. (Author).

Energy Technology Data Exchange (ETDEWEB)

Our major goal of our work has been to develop and use NMR techniques to study how methanogenic archaebacteria deal with osmotic stress with the hope of providing insights into increasing the salt tolerance of other cells. The project has three main sections: (i) in vivo studies of methanogens; (ii) use of [sup l3]C- and [sup l5]N- labeled potential precursors and in vitro analyses of specific label uptake for elucidation of osmolyte dynamics and biosynthetic pathways of osmolytes in these organisms, and isolation of key biosynthetic enzymes; and (iii) collaborative studies on identification of organic solutes in other methanogens.

Energy Technology Data Exchange (ETDEWEB)

Studies of various established human bladder and renal carcinoma cell lines cultured in vitro demonstrated the presence of specific, saturable, high affinity binding sites for /sup 125/I-labeled human interferon Beta ser IFN-beta ser). This recombinant produced interferon labeled with approximately one atom of /sup 125/I/molecule of IFN expressed minimal or no loss of antiviral activity. A single class of binding sites (1000-2000/cell) with an affinity constant of 10(10)-10(11) L/M was measured at 4 degrees C for cells exhibiting widely different sensitivity to the antiproliferative effect of IFN-beta ser. Major fluctuations in the binding of /sup 125/I-labeled IFN-beta ser to cellular receptors were observed during in vitro proliferation of four of five cell lines examined. A significant decrease (P less than 0.001) in specific binding was observed 48 h after cultures were established. Cell cycle analysis suggested that ...

International Nuclear Information System (INIS)

The patients studied were referred for routine abscess localization with indium-111 labelled autologous leukocytes. One series (n=15) was studied at 1 day after reinjection of labelled cells and a second series (n=9) was studied between 4 hours and 4 days after reinjection of labelled cells. The leukocytes were isolated by a gravity sedimentation method and labelled with In-111 oxinate according to Roevekamp and co-workers. The labelled cell suspension administered to the patient contained between 11 and 15 MegaBq (300-400 microCi). The indium-111 concentration in the liver and spleen was quantitated using a scintillation camera and an on-line computer system. Phantom studies were carried out to obtain conversion factors from geometric mean counts to MegaBq of activity for both liver and spleen. Once determined, the amount of activity in liver and spleen was corrected for physical ...

Energy Technology Data Exchange (ETDEWEB)

Insulin receptors on the surface of isolated rat adipocytes were photoaffinity labeled at 12 degrees C with the iodinated photoreactive insulin analogue, 125I-B2 (2-nitro-4-azidophenylacetyl)-des-PheB1-insulin, and the pathways in the intracellular processing of the labeled receptors were studied at 37 degrees C. During 37 degrees C incubations, the labeled 440-kDa insulin receptors were continuously internalized (as assessed by trypsin inaccessibility) and degraded such that up to 50% of the initially labeled receptors were lost by 120 min. Metabolic poisons (0.125-0.75 mM 2,4-dinitrophenol (DNP) and 1-10 mM NaF), which led to dose-dependent depletion of adipocyte ATP pools, inhibited receptor loss, and caused up to 3-fold increase in intracellular receptor accumulation. This effect was due to inhibition of intracellular receptor degradation, and there was no apparent effect of the metabolic poisons on ...

Energy Technology Data Exchange (ETDEWEB)

Bone formation and resorption have been measured in patients with idiopathic osteoporosis by histomorphometry of 7.5-mm trephine biopsies and in the whole body by 85Sr radiotracer methodology and calcium balances. The studies were synchronized and most were preceded by double in vivo tetracycline labeling. Correlations between histological and kinetic bone formation indices were better when better when based on the extent of double tetracycline labels than on measurements of osteoid by visible light microscopy. Correction of the kinetic data for long-term exchange, using 5 months' serial whole body counting of retained 85Sr, improved the fit of the kinetic to the histological data. A statistical analysis of the measurement uncertainties showed that the residual scatter in the best correlations (between exchange-corrected bone formation rates and double-labeled osteoid surface indices) could be attributed to ...