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Sample records for 2-hour urokinase regime

  1. Molecular-specific urokinase antibodies

    Atassi, M. Zouhair (Inventor); Morrison, Dennis R. (Inventor)

    2009-01-01

    Antibodies have been developed against the different molecular forms of urokinase using synthetic peptides as immunogens. The peptides were synthesized specifically to represent those regions of the urokinase molecules which are exposed in the three-dimensional configuration of the molecule and are uniquely homologous to urokinase. Antibodies are directed against the lysine 158-isoleucine 159 peptide bond which is cleaved during activation from the single-chain (ScuPA) form to the bioactive double chain (54 KDa and 33 KDa) forms of urokinase and against the lysine 135 lysine 136 bond that is cleaved in the process of removing the alpha-chain from the 54 KDa form to produce the 33 KDa form of urokinase. These antibodies enable the direct measurement of the different molecular forms of urokinase from small samples of conditioned medium harvested from cell cultures.

  2. Conformational regulation of urokinase receptor function

    Gårdsvoll, Henrik; Jacobsen, Benedikte; Kriegbaum, Mette C;

    2011-01-01

    The urokinase-type plasminogen activator receptor (uPAR) is a glycolipid-anchored membrane protein with an established role in focalizing uPA-mediated plasminogen activation on cell surfaces. Distinct from this function, uPAR also modulates cell adhesion and migration on vitronectin-rich matrices...

  3. Thrombolytic treatment for acute ischemic cerebral stroke: intraarterial urokinase infusion vs. intravenous heparin and urokinase infusion

    To evaluate the efficacy and limitation of intra-arterial urokinase (IAUK) infusion for treatment of acute cerebral stroke. Twenty-seven acute cerebral stroke patients treated with IAUK infusion within six hours of stroke onset were reviewed. All patients showed normal initial brain findings on CT. In 21 patients, urokinase(5-15 x 105IU) was administered through a microcatheter placed into or proximal to occluded segment. Mechanical disruption of thrombus by guidewire was performed in 17 patients. Angiographic and clinical responses and complications after IAUK infusion, were evaluated and the results were compared with those of intravenous heparin(N=19) and urokinase infusion(N=19). Complete or partial angiographic recanalization of occluded segment was found in 18 patients (67%), and neurologic improvement was followed in 14 patients(52%). The degree of improvement on the stroke scale score after IAUK infusion was statistically more significant(p<0.05) than that shown after intravenous heparin and urokinase infusion. Complications after IAUK infusion were large(15%) and small amount intracerebral hemorrhage(15%), contrast leakage into brain parenchyma(11%), and gastrointestinal bleeding(4%). Between the IAVK and the intravenous urokinase infusion group, differences in extent and types of complications were statistically insignificant, but were significantly higher in those two groups than in the intravenous heparin infusion group. IAUK infusion may be effective for the treatment of acute cerebral stroke

  4. Antibodies Against Three Forms of Urokinase

    Morrison, Dennis R.; Atassi, M. Zouhair

    2007-01-01

    Antibodies that bind to preselected regions of the urokinase molecule have been developed. These antibodies can be used to measure small quantities of each of three molecular forms of urokinase that could be contained in microsamples or conditioned media harvested from cultures of mammalian cells. Previously available antibodies and assay techniques do not yield both clear distinctions among, and measurements of, all three forms. Urokinase is a zymogen that is synthesized in a single-chain form, called ScuPA, which is composed of 411 amino acid residues (see figure). ScuPA has very little enzyme activity, but it can be activated in two ways: (1) by cleavage of the peptide bond lysine 158/isoleucine 159 and the loss of lysine 158 to obtain the high molecular-weight (HMW) form of the enzyme or (2) by cleavage of the bond lysine 135/lysine 136 to obtain the low-molecular-weight (LMW) form of the enzyme. The antibodies in question were produced in mice and rabbits by use of peptides as immunogens. The peptides were selected to obtain antibodies that bind to regions of ScuPA that include the lysine 158/isoleucine 159 and the lysine 135/lysine 136 bonds. The antibodies include monoclonal and polyclonal ones that yield indications as to whether either of these bonds is intact. The polyclonal antibodies include ones that preferentially bind to the HMW or LMW forms of the urokinase molecule. The monoclonal antibodies include ones that discriminate between the ScuPA and the HMW form. A combination of these molecular-specific antibodies will enable simultaneous assays of the ScuPA, HMW, and LMW forms in the same specimen of culture medium.

  5. A composite role of vitronectin and urokinase in the modulation of cell morphology upon expression of the urokinase receptor

    Hillig, Thore; Engelholm, Lars H; Ingvarsen, Signe;

    2008-01-01

    The urokinase receptor, urokinase receptor (uPAR), is a glycosylphosphatidylinositol-anchored membrane protein engaged in pericellular proteolysis and cellular adhesion, migration, and modulation of cell morphology. A direct matrix adhesion is mediated through the binding of uPAR to vitronectin, ...

  6. Expression of urokinase receptors by human trophoblast. A histochemical and ultrastructural analysis

    Multhaupt, H A; Mazar, A; Cines, D B;

    1994-01-01

    vivo has not been examined. EXPERIMENTAL DESIGN: Immunohistochemistry and immunoelectron microscopy were used to characterize the expression of urokinase receptors by villous and extravillous trophoblast at several points in gestation. RESULTS: Urokinase receptors were expressed in a polarized fashion...

  7. Regulation of urokinase by cellular receptors and inhibitors

    Several cell types display binding sites for 125I-urokinase which in certain cases are occupied with endogenous urokinase. These sites appear to focus urokinase at cell surfaces and hence may participate in tissue matrix destruction and cell invasion. Using immunofluorescence double labeling, the author shows that the receptor-bound urokinase present on human foreskin fibroblasts and HT1080 human fibrosarcoma cells is colocalized with vinculin, an intracellular actin-binding protein that is deposited at cell to substratum focal adhesion sites. Thus, this indicates linkage of the plasminogen/plasmin system both to sites of cell adhesion and to the cytoskeleton. He furthermore reports that neither EGF, TGFβ or PDGF significantly altered the shape or intensity of the receptor-bound urokinase clusters but that thrombin, at mitogenic doses, caused a disappearance of the urokinase strands and a loss or gross alteration of the underlying focal adhesion plaques, as indicated by immunofluorescence staining for vinculin and talin, and by interference reflection microscopy. These observations suggest that thrombin may be a unique effector of cell adhesion, shape and movement. He used a quantitative in vitro invasion assay to study the role of plasminogen activator inhibitors type 1 and 2 (PAI-1, PAI-2) and protease nexin (PN1) in basement membrane (BM) invasion by 125I-iododeoxyuridine-labeled HT 1080 cells. The results obtained showed that 5 μg/ml of PAI-1, PAI-2 and PN1 preadsorbed to the BM completely blocked HT1080 invasion. On the contrary an anti-PAI-1 monoclonal antibody induced an approximately two-fold increase in invasion. 125I-fibrinogen was polymerized on the amnion BM and the fibrinolytic activity of the cells was measured under the invasion assay conditions by measuring the radioactivity in the culture medium at different time points

  8. Signaling through urokinase and urokinase receptor in lung cancer cells requires interactions with beta1 integrins.

    Tang, Chi-Hui; Hill, Marla L; Brumwell, Alexis N; Chapman, Harold A; Wei, Ying

    2008-11-15

    The urokinase receptor (uPAR) is upregulated upon tumor cell invasion and correlates with poor lung cancer survival. Although a cis-interaction with integrins has been ascribed to uPAR, whether this interaction alone is critical to urokinase (uPA)- and uPAR-dependent signaling and tumor promotion is unclear. Here we report the functional consequences of point mutations of uPAR (H249A-D262A) that eliminate beta1 integrin interactions but maintain uPA binding, vitronectin attachment and association with alphaV integrins, caveolin and epidermal growth factor receptor. Disruption of uPAR interactions with beta1 integrins recapitulated previously reported findings with beta1-integrin-derived peptides that attenuated matrix-dependent ERK activation, MMP expression and in vitro migration by human lung adenocarcinoma cell lines. The uPAR mutant cells acquired enhanced capacity to adhere to vitronectin via uPAR-alphaVbeta5-integrin, rather than through the uPAR-alpha3beta1-integrin complex and they were unable to initiate uPA signaling to activate ERK, Akt or Stat1. In an orthotopic lung cancer model, uPAR mutant cells exhibited reduced tumor size compared with cells expressing wild-type uPAR. Taken together, the results indicate that uPAR-beta1-integrin interactions are essential to signals induced by integrin matrix ligands or uPA that support lung cancer cell invasion in vitro and progression in vivo. PMID:18940913

  9. The significance of the serum CG level of 2 hours after meal in hyperthyroid patients

    To observe the liver condition of hyperthyroid patients with different course of disease, the levels of serum CG over night fasting and of 2 hours after meal in 132 hyperthyroid patients and 50 normal persons were measured by RIA. Hyperthyroid patients were divided into 3 groups by their different course of disease. For normal persons and the group whose course of disease within half year there was no significant difference in CG levels of fasting and 2 hours after meal. For the group whose course of disease in period of 0.5-2 years the CG level of 2 hours after meal were over the normal range (P<0.01), but no difference was found in CG level of fasting. For the group whose course of disease over 2 years, the CG levels of fasting and 2 hours after meal were all over the normal range (P<0.01, P<0.001), CG level of the 2 hours after meal was much higher. The results suggested that CG level measured 2 hours after meal was more sensitive than that after over night fasting in hyperthyroid patients with hepatocellular damage, and the damage degree paralleled to their courses of disease

  10. The pro-urokinase plasminogen-activation system in the presence of serpin-type inhibitors and the urokinase receptor

    Behrendt, Niels; List, Karin; Andreasen, Peter A; Danø, Keld

    The reciprocal pro-enzyme activation system of plasmin, urokinase-type plasminogen activator (uPA) and their respective zymogens is a potent mechanism in the generation of extracellular proteolytic activity. Plasminogen activator inhibitor type 1 (PAI-1) acts as a negative regulator. This system ...

  11. UVB increases urokinase-type plasminogen activator receptor (uPAR) expression.

    Marschall, C; Lengyel, E; Nobutoh, T; Braungart, E; Douwes, K; Simon, A; Magdolen, V; Reuning, U; Degitz, K

    1999-07-01

    Keratinocytes synthesize and secrete urokinase-type plasminogen activator, which binds to its specific receptor on keratinocytes. When bound to urokinase-type plasminogen activator receptor, urokinase-type plasminogen activator proteolytically converts surface bound plasminogen to plasmin, which in turn cleaves many extracellular components leading to pericellular proteolysis. The activation of the urokinase system has been observed during re-epithelialization of skin wounds and in lesions of the autoimmune blistering skin disease pemphigus. As pemphigus is photoinducible, we investigated the effect of ultraviolet B on urokinase-type plasminogen activator and urokinase-type plasminogen activator receptor expression in the epidermal keratinocyte cell line A431. Ultraviolet B increased cellular and secreted urokinase-type plasminogen activator protein (enzyme-linked immunosorbent assay) and urokinase-type plasminogen activator receptor cell surface expression (flow cytometry) 24 h postirradiation. Northern blot analysis indicated that ultraviolet B increased urokinase-type plasminogen activator receptor mRNA. Compared with a more rapid mRNA induction by epidermal growth factor (maximal after 4 h) the ultraviolet B response was maximal after 24 h and prolonged up to 36 h. The mRNA induction was not dependent on protein synthesis as judged by cycloheximide incubation. Ultraviolet B did not influence urokinase-type plasminogen activator receptor mRNA stability (actinomycin D incubation). A transiently transfected chloramphenicol acetyltransferase-reporter construct containing a -398/+51 urokinase-type plasminogen activator receptor promoter fragment was activated when cells were exposed to ultraviolet B. This induction was almost completely abolished by mutating a -182/-176 AP-1 binding sequence. Ultraviolet B increased the binding capacity at this AP-1 motif in electrophoretic mobility shift assays. These data identify a distinct transcriptional mechanism by which

  12. Characterization of urokinase receptor expression by human placental trophoblasts.

    Zini, J M; Murray, S C; Graham, C H; Lala, P K; Karikó, K; Barnathan, E S; Mazar, A; Henkin, J; Cines, D B; McCrae, K R

    1992-06-01

    The processes of implantation and placentation are both dependent on the invasion and remodeling of the uterine endometrium and vasculature by trophoblasts. Because the secretion and autocrine binding of urokinase (uPA) appears to be a common mechanism used by cells to facilitate plasmin-dependent tissue invasion, we measured the production of uPA and expression of uPA receptors by trophoblasts. Prourokinase bound specifically, reversibly, and with high affinity to cultured trophoblasts, via the uPA epidermal growth factor-like domain. Trophoblasts derived from two first-trimester placentae bound more prourokinase than cells isolated from term placentae. Furthermore, in vitro differentiation of cultured cytotrophoblasts into syncytiotrophoblasts was associated with diminished expression of urokinase receptors and a parallel decrease in the cellular content of uPA receptor mRNA. Trophoblasts also secreted prourokinase and plasminogen activator inhibitors types 1 and 2 (PAI-1 and PAI-2). Although prourokinase was secreted in amounts sufficient to endogenously saturate trophoblast uPA receptors, trophoblasts secreted greater amounts of PAI-1 and PAI-2 than uPA, and no net plasminogen activator activity was detected in trophoblast conditioned medium. In contrast, plasminogen added directly to cultured trophoblasts was readily converted to plasmin. Although the invasion and remodeling of uterine tissues by trophoblasts is a complex process dependent on several proteases of varying specificity, our findings suggest that the expression and modulation of urokinase receptors on the trophoblast cell surface may play an important role in this process. PMID:1316787

  13. Urokinase receptor forms in serum from non-small cell lung cancer patients

    Almasi, Charlotte Elberling; Christensen, Ib Jarle; Høyer-Hansen, Gunilla; Danø, Keld; Pappot, Helle; Dienemann, Hendrik; Muley, Thomas

    2011-01-01

    To study the prognostic impact of the different forms of the receptor for urokinase plasminogen activator (uPAR) in serum from 171 non-small cell lung cancer (NSCLC) patients.......To study the prognostic impact of the different forms of the receptor for urokinase plasminogen activator (uPAR) in serum from 171 non-small cell lung cancer (NSCLC) patients....

  14. Plasma soluble urokinase plasminogen activator receptor in children with urinary tract infection

    Wittenhagen, Per; Andersen, Jesper Brandt; Hansen, Anita; Lindholm, Lone; Rønne, Frederik Malmborg; Theil, Jørn; Tvede, Michael; Eugen-Olsen, Jesper

    2011-01-01

    In this prospective study we investigated the role of plasma levels of soluble urokinase plasminogen activator receptor (suPAR) in children with urinary tract infection.......In this prospective study we investigated the role of plasma levels of soluble urokinase plasminogen activator receptor (suPAR) in children with urinary tract infection....

  15. Purification of urokinase by combined cation exchanger and affinity chromatographic cartridges.

    Hou, K C; Zaniewski, R

    1990-02-23

    Crude urokinase from human urine processed through foam flotation and ammonium sulfate precipitation containing 720 National Health Institute Committee on Thrombolytic Agents U/mg activity was purified by an SP cation exchanger followed by a zinc-chelated affinity chromatographic cartridge. The cartridges were of a radial-flow type formed by using acrylic and cellulose composite matrices. The high rigidity of the matrix structure permits fast flow of protein solutions (liters per minute) and thus allows processing of a large volume of crude urokinase under low operating pressures. A greater than six-fold increase in specific enzyme activity of urokinase was achieved by adsorbing and eluting 1 l of a 3 mg/ml crude urokinase solution on an SP cartridge. The eluent was further purified by passing through a zinc-chelated affinity cartridge to achieve greater than a eighteen-fold increase in urokinase specific activity. This report demonstrates the combined use of a cation exchanger with zinc-chelated chromatographic cartridges in purifying urokinase on a relatively large scale. The relationship between the amount of zinc chelated in the matrix to its effect on urokinase purification is also discussed. PMID:2329161

  16. Clinical study of urokinase-bound fibrocollagenous tubes.

    Senatore, F; Bernath, F; Meisner, K

    1986-02-01

    Dacron-reinforced fibrocollagenous tubes (FCT) were synthesized from canine mongrels using the mandril-rod technique in order to develop a small diameter (i.e., 4 mm i.d.) vascular graft. They were rendered fibrinolytic by immobilizing urokinase on to the inner surface of the tubes. Urokinase-bound fibrocollagenous tubes (UK-FCT), control FCTs (i.e., no bound enzyme), Perloff grafts (Dr. Perloff, Department of Surgery, University Medical Center, Sidney Australia, has developed a mandril-derived collagenous tube from goats. Samples were implanted for comparative purposes.) and autogeneous saphenous veins, were interposed in the carotid or femoral artery in chronic studies involving 21 canine mongrels. On the basis of Doppler auscultation and palpation, the UK-FCTs were statistically more patent than other candidate prostheses. Fibrin degradation product (FDP) increased in the dogs' systemic circulation with a postoperative peak of 5 days. The host's increase in fibrinolytic activity was shown to be local to the anastamosis. A carotid arterial extracorporeal shunt was designed to evaluate acute patency. Results indicated a rapid thrombosis but no platlet or fibrin adherence to the graft surface was observed, as evidenced by scanning electron microscopy. PMID:2937788

  17. Structural investigations of recombinant urokinase growth factor-like domain.

    Beloglazova, I B; Beabealashvilli, R Sh; Gursky, Ya G; Bocharov, E V; Mineev, K S; Parfenova, E V; Tkachuk, V A

    2013-05-01

    Urokinase-type plasminogen activator (uPA) is a serine protease that converts the plasminogen zymogen into the enzymatically active plasmin. uPA is synthesized and secreted as the single-chain molecule (scuPA) composed of an N-terminal domain (GFD) and kringle (KD) and C-terminal proteolytic (PD) domains. Earlier, the structure of ATF (which consists of GFD and KD) was solved by NMR (A. P. Hansen et al. (1994) Biochemistry, 33, 4847-4864) and by X-ray crystallography alone and in a complex with the soluble form of the urokinase receptor (uPAR, CD87) lacking GPI (C. Barinka et al. (2006) J. Mol. Biol., 363, 482-495). According to these data, GFD contains two β-sheet regions oriented perpendicularly to each other. The area in the GFD responsible for binding to uPAR is localized in the flexible Ω-loop, which consists of seven amino acid residues connecting two strings of antiparallel β-sheet. It was shown by site-directed mutagenesis that shortening of the Ω-loop length by one amino acid residue leads to the inability of GFD to bind to uPAR (V. Magdolen et al. (1996) Eur. J. Biochem., 237, 743-751). Here we show that, in contrast to the above-mentioned studies, we found no sign of the β-sheet regions in GFD in our uPA preparations either free or in a complex with uPAR. The GFD seems to be a rather flexible and unstructured domain, demonstrating in spite of its apparent flexibility highly specific interaction with uPAR both in vitro and in cell culture experiments. Circular dichroism, tryptophan fluorescence during thermal denaturation of the protein, and heteronuclear NMR spectroscopy of ¹⁵N/¹³C-labeled ATF both free and in complex with urokinase receptor were used to judge the secondary structure of GFD of uPA. PMID:23848154

  18. An experimental investigation on the concentration of selective infusion of urokinase in superacute cerebral embolism in dog

    Objective: To study the effect of thrombolysis of intra-arterial infusion of different concentrations of urokinase (UK) in dogs. Methods: 25 healthy crossbreeding dogs were divided into five groups, with five dogs in each group. Every dog was injected with self-thrombus from carotids internal artery to embolize its distal part or branches. Treatment with different concentrations of UK was initiated 2 hours after setting up the model of cerebral embolism by carotids internal artery. The dose of each group was: A(control group), 0.9% physiological sodium chloride solution; B, 1200 U/ml UK; C, 6000 U/ml UK; D, 12000 U/ml UK; E, 60000 U/ml UK. Angiography and CT scannings were performed before and after thrombolysis. Pathologic examination was performed 24 hours after embolism. Results: The rate of recanalization of groups A, B were 0 but 100% for groups C, D and E, Judged by angiographies after thrombolysis, group C, D and E had significantly better reperfusion compared with group A, B(P<0.0001). Among groups C, D and E there were no significant differences but with a large bleeding in four dogs of group E after recanalization by CT scanning. Conclusions: Intra-arterial thrombolysis is an effective method to treat the superacute cerebral embolism but with a range of safe and effective concentration of UK, 6000 U/ml-12000 U/ml, reaching high rate of thrombolysis, and low rate of hemorrhage

  19. Bicyclic Peptide Inhibitor of Urokinase-Type Plasminogen Activator

    Roodbeen, Renée; Paaske, Berit; Jiang, Longguang;

    2013-01-01

    The development of protease inhibitors for pharmacological intervention has taken a new turn with the use of peptidebased inhibitors. Here, we report the rational design of bicyclic peptide inhibitors of the serine protease urokinase-type plasminogen activator (uPA), based on the established...... monocyclic peptide, upain-2. It was successfully converted to a bicyclic peptide, without loss of inhibitory properties. The aim was to produce a peptide cyclised by an amide bond with an additional stabilising across-the-ring covalent bond. We expected this bicyclic peptide to exhibit a lower entropic...... burden upon binding. Two bicyclic peptides were synthesised with affinities similar to that of upain-2, and their binding energetics were evaluated by isothermal titration calorimetry. Indeed, compared to upain-2, the bicyclic peptides showed reduced loss of entropy upon binding to uPA. We also...

  20. Urokinase and type I plasminogen activator inhibitor production by normal human hepatocytes: modulation by inflammatory agents.

    Busso, N; Nicodeme, E; Chesne, C; Guillouzo, A; Belin, D; Hyafil, F

    1994-07-01

    We examined the effects of inflammatory cytokines (interleukin-1 beta, tumor necrosis factor-alpha and transforming growth factor-beta) on the plasminogen activator system (urokinase, tissue-type plasminogen activator, type 1 plasminogen activator inhibitor) in primary cultures of human hepatocytes. We show that interleukin-1 beta and tumor necrosis factor-alpha increase urokinase-type plasminogen activator production, reinforcing the concept that increased urokinase production is associated with inflammatory processes. By contrast, the same agents (i.e., interleukin-1 beta and tumor necrosis factor-alpha) do not stimulate plasminogen activator inhibitor type 1 production. This latter observation rules out hepatocytes as a major cellular source of plasmatic plasminogen activator inhibitor type 1 during acute-phase-related responses. Among the inflammatory agents used, transforming growth factor-beta was found to be the most effective modulator of both urokinase-type plasminogen activator and plasminogen activator inhibitor type 1, inducing severalfold increases of activity of urokinase-type plasminogen activator, antigen and the corresponding mRNA and increasing plasminogen activator inhibitor type 1 antigen and mRNA levels. Urokinase-type plasminogen activator and plasminogen activator inhibitor type 1 modulation by transforming growth factor-beta may play a critical role in hepatic pathophysiology. PMID:8020888

  1. Macroeconomic regimes

    Baele, L.T.M.; Bekaert, G.R.J.; Cho, S.; Inghelbrecht, K.; Moreno, A.

    2015-01-01

    A New-Keynesian macro-model is estimated accommodating regime-switching behavior in monetary policy and macro-shocks. A key to our estimation strategy is the use of survey-based expectations for inflation and output. Output and inflation shocks shift to the low volatility regime around 1985 and 1990

  2. Prolonged radiation damage in rat colon and urokinase expression in epithelium

    Hayashida, Masayuki; Minami, Kazunori; Okimoto, Tomoaki [Nagasaki Univ. (Japan). School of Medicine; Shichijo, Kazuko; Matsuu, Mutsumi; Nakayama, Toshiyuki; Sekine, Ichiro

    2001-12-01

    Although radiation therapy plays important role in the treatment of gynecological tumors, it may cause radiation injury as a late effect. Several recent reports show that urokinase such as urokinase type plasminogen activator (uPA) contributes to the repair of ulcerative lesions of the colon epithelium. We studied radiation induced enterocolitis using rat animal models. Seventy-two female Wistar rats were irradiated by a single fraction dose of 36 Gy at laparotomy. Histological changes and activity of urokinase system were investigated after irradiation. Ulcers were observed in irradiated field in 12 of 19 animals (63%) even at 60th week after irradiation. Urokinase expressions were observed in the margins of active ulcer. Urokinase was thought to play important role in exacerbation of ulcer formation. Expression of uPA was also observed in submucosal glands. Ischaemic changes were not observed in irradiated colon despite sclerosing vasculitis. It is suggested that uPA played reciprocal roles in radiation induced enterocolitis: healing and aggravation of ulcer. (author)

  3. Application of Molecular Modeling to Urokinase Inhibitors Development

    V. B. Sulimov

    2014-01-01

    Full Text Available Urokinase-type plasminogen activator (uPA plays an important role in the regulation of diverse physiologic and pathologic processes. Experimental research has shown that elevated uPA expression is associated with cancer progression, metastasis, and shortened survival in patients, whereas suppression of proteolytic activity of uPA leads to evident decrease of metastasis. Therefore, uPA has been considered as a promising molecular target for development of anticancer drugs. The present study sets out to develop the new selective uPA inhibitors using computer-aided structural based drug design methods. Investigation involves the following stages: computer modeling of the protein active site, development and validation of computer molecular modeling methods: docking (SOL program, postprocessing (DISCORE program, direct generalized docking (FLM program, and the application of the quantum chemical calculations (MOPAC package, search of uPA inhibitors among molecules from databases of ready-made compounds to find new uPA inhibitors, and design of new chemical structures and their optimization and experimental examination. On the basis of known uPA inhibitors and modeling results, 18 new compounds have been designed, calculated using programs mentioned above, synthesized, and tested in vitro. Eight of them display inhibitory activity and two of them display activity about 10 μM.

  4. Two distinct expression patterns of urokinase, urokinase receptor and plasminogen activator inhibitor-1 in colon cancer liver metastases

    Illemann, Martin; Bird, Nigel; Majeed, Ali;

    2009-01-01

    Metastatic growth and invasion by colon cancer cells in the liver requires the ability of the cancer cells to interact with the new tissue environment. Plasmin(ogen) is activated on cell surfaces by urokinase-type PA (uPA), and is regulated by uPAR and plasminogen activator inhibitor-1 (PAI-1......). To compare the expression patterns of uPA, uPAR and PAI-1 in colon cancer with that in their liver metastases, we analysed matched samples from 14 patients. In all 14 primary colon cancers, we found upregulation of uPAR, uPA mRNA and PAI-1 in primarily stromal cells at the invasive front. In 5 of the 14......, whereas 8 of the remaining 9 showed direct contact between the cancer cells and the liver parenchyma. We conclude that there are 2 distinct patterns of expression of uPAR, uPA and PAI-1 in colon cancer liver metastases and that these correlate closely with 2 morphological growth patterns. These findings...

  5. Adsorption and Step Elution of Urokinase Using, Affinity Chromatography -Comparison of Data with Rate Model Simulation

    MohammadRezaAboudzadehRovais; JiawenZhu; BinWu

    2004-01-01

    A non-equilibrium chromatographic rate model was employed to simulate the affinity chromatography of urokinase. The chromatography process was developed to a yield of high purity product of urokinase from crude materials. The affinity gel used in the process was prepared by an epichlorohydrin-activation method using epichlorohydrin activated Sepharose 4B as a matrix and p-aminobenzamidine as a ligand. The chromatographic process were numerically simulated and analyzed with the aid of VERSE-LC computer simulator. Considering the basic principles, rate model with the back mixing in column inlet was utilized in simulating and studying the effect of the column inlet pattern on other parameters. Comparison of the simulation results with the experimental data showed that the rate model can be used to describe the affinity chromatography of urokinase in a fixed bed column with satisfactory accuracy.

  6. Efficacy of 2-hour post glucose insulin levels in predicting insulin resistance in polycystic ovarian syndrome with infertility

    Saxena, Pikee; Prakash, Anupam; Nigam, Aruna

    2011-01-01

    BACKGROUND: Insulin resistance (IR) is central to the pathogenesis of polycystic ovarian syndrome (PCOS), but tests for determining IR are elaborate, tedious and expensive. AIMS: To evaluate if “2-hour post-glucose insulin level” is an effective indicator of IR and can aid in diagnosing IR in infertile PCOS women. SETTINGS AND DESIGN: Observational study at infertility clinic of a tertiary care center. MATERIALS AND METHODS: 50 infertile women with PCOS and 20 females with tubal/male factor infertility were evaluated for the presence of IR, as defined by the fasting/2-hour post-glucose insulin levels cutoffs of >25/>41 μU/mL, respectively. The clinical, metabolic and endocrinologic profile was determined in both the groups. STATISTICAL ANALYSIS: Statistical analysis was performed using SPSS (Chicago, IL, USA). RESULTS: Body mass index, post load glucose, insulin, glucose/insulin ratio, area under curve (AUC) of glucose and insulin and insulinogenic index were significantly lower in the controls as compared to the PCOS group. “2-hour post-glucose insulin levels” were elevated in 88% of PCOS individuals but were normal in all females not suffering from PCOS. These levels significantly correlated with AUC of glucose and insulin, and insulinogenic index and inversely correlated with 2-hour glucose to insulin ratio (r=0.827, 0.749 and –0.732, respectively). CONCLUSIONS: “2-hour post-glucose insulin levels” appears to be a good indicator of IR. It can be a useful tool, especially in low resource setting where a single sample can confirm the diagnosis, thus reducing cost and repeat visits. PMID:21772735

  7. Efficacy of 2-hour post glucose insulin levels in predicting insulin resistance in polycystic ovarian syndrome with infertility

    Pikee Saxena

    2011-01-01

    Full Text Available Background : Insulin resistance (IR is central to the pathogenesis of polycystic ovarian syndrome (PCOS, but tests for determining IR are elaborate, tedious and expensive. Aims : To evaluate if "2-hour post-glucose insulin level" is an effective indicator of IR and can aid in diagnosing IR in infertile PCOS women. Settings and Design : Observational study at infertility clinic of a tertiary care center. Materials and Methods : 50 infertile women with PCOS and 20 females with tubal/male factor infertility were evaluated for the presence of IR, as defined by the fasting/2-hour post-glucose insulin levels cutoffs of >25/>41 μU/mL, respectively. The clinical, metabolic and endocrinologic profile was determined in both the groups. Statistical Analysis : Statistical analysis was performed using SPSS (Chicago, IL, USA. Results : Body mass index, post load glucose, insulin, glucose/insulin ratio, area under curve (AUC of glucose and insulin and insulinogenic index were significantly lower in the controls as compared to the PCOS group. "2-hour post-glucose insulin levels" were elevated in 88% of PCOS individuals but were normal in all females not suffering from PCOS. These levels significantly correlated with AUC of glucose and insulin, and insulinogenic index and inversely correlated with 2-hour glucose to insulin ratio (r=0.827, 0.749 and −0.732, respectively. Conclusions : "2-hour post-glucose insulin levels" appears to be a good indicator of IR. It can be a useful tool, especially in low resource setting where a single sample can confirm the diagnosis, thus reducing cost and repeat visits.

  8. Estuary regime

    The primary aim of this research into estuary regime is to provide engineers with an improved method of predicting the long term evolutionary effects of major engineering changes in estuaries. Of specific interest to the Department of Trade and Industry's renewable energy R and D programme is the long term impact of tidal energy barrages on estuarine morphology. It is considered that the approach being taken is the most appropriate line to follow in terms of developing an applicable estuary regime predictive capability. As a consequence it is considered that any further regime model development should be complimentary to this approach. In addition to model development there is still an enormous amount of basic research required in examining sediment transport processes within the estuarine environment. Recently developed models for predicting the motion of fluidised mud on inter-tidal and sub-tidal banks, an important process within the estuarine sediment regime, requires specific field data calibration and validation. The impacts of wave action on sediment mobility on estuarine banks is a further area requiring field measurements. It is recommended that the opportunity be taken to obtain a set of measurements at a site following construction of major engineering works to provide data for future verification of a regime model. Possibilities exist, for example, on the Tees Estuary for such measurements. (author)

  9. Activation of human pro-urokinase by unrelated proteases secreted by Pseudomonas aeruginosa : LasB and protease IV activate human pro-urokinase

    Beaufort, Nathalie; Seweryn, Paulina; De Bentzmann, Sophie; Tang, Aihua; Kellermann, Josef; Grebenchtchikov, Nicolai; Schmitt, Manfred; Sommerhoff, Christian,; Pidard, Dominique; Magdolen, Viktor

    2010-01-01

    Abstract Pathogenic bacteria, including Pseudomonas aeruginosa, interact with and engage the host plasminogen (Plg) activation system, which encompasses the urokinase(uPA)-type Plg activator, and is involved in extracellular proteolysis, including matrilysis and fibrinolysis. We hypothesized that secreted bacterial proteases might contribute to the activation of this major extracellular proteolytic system, thereby participating in bacterial dissemination. We report that LasB, a...

  10. Regime change?

    Following the 1998 nuclear tests in South Asia and later reinforced by revelations about North Korean and Iraqi nuclear activities, there has been growing concern about increasing proliferation dangers. At the same time, the prospects of radiological/nuclear terrorism are seen to be rising - since 9/11, concern over a proliferation/terrorism nexus has never been higher. In the face of this growing danger, there are urgent calls for stronger measures to strengthen the current international nuclear nonproliferation regime, including recommendations to place civilian processing of weapon-useable material under multinational control. As well, there are calls for entirely new tools, including military options. As proliferation and terrorism concerns grow, the regime is under pressure and there is a temptation to consider fundamental changes to the regime. In this context, this paper will address the following: Do we need to change the regime centered on the Treaty on the Nonproliferation of Nuclear Weapons (NPT) and the International Atomic Energy Agency (IAEA)? What improvements could ensure it will be the foundation for the proliferation resistance and physical protection needed if nuclear power grows? What will make it a viable centerpiece of future nonproliferation and counterterrorism approaches?

  11. Effects of acute and 2-hour postphysical activity on the estimation of body fat made by the bod pod.

    Harrop, Bradley J; Woodruff, Sarah J

    2015-06-01

    The Bod Pod has been found to be reliable/valid against several criterion methods, including hydrostatic weighing and dual-energy x-ray absorptiometry, and under different conditions, such as clothing, dehydrated states, and body temperature changes. However, questions remain regarding the effects of an acute bout of exercise. Therefore, the purpose was to determine the effects of an acute bout of exercise on the estimations made by the Bod Pod. Participants (15 men and 22 women) were of age 18-27 years and were currently exercising. Baseline Bod Pod measures were completed followed by a 30-minute cycling trial at 75% of maximum heart rate. Bod Pod measures were taken immediately after exercise and 2 hours after exercise. Differences between men and women were found at baseline between height (p temperature (p temperature (p affect the prediction 2 hours after exercise in women. PMID:25756324

  12. Efficacy of 2-hour post glucose insulin levels in predicting insulin resistance in polycystic ovarian syndrome with infertility

    Pikee Saxena; Anupam Prakash; Aruna Nigam

    2011-01-01

    Background : Insulin resistance (IR) is central to the pathogenesis of polycystic ovarian syndrome (PCOS), but tests for determining IR are elaborate, tedious and expensive. Aims : To evaluate if "2-hour post-glucose insulin level" is an effective indicator of IR and can aid in diagnosing IR in infertile PCOS women. Settings and Design : Observational study at infertility clinic of a tertiary care center. Materials and Methods : 50 infertile women with PCOS and 20 females with tubal/male fact...

  13. Dietary Fatty Acids Differentially Associate with Fasting Versus 2-Hour Glucose Homeostasis: Implications for The Management of Subtypes of Prediabetes.

    Guess, Nicola; Perreault, Leigh; Kerege, Anna; Strauss, Allison; Bergman, Bryan C

    2016-01-01

    Over-nutrition has fuelled the global epidemic of type 2 diabetes, but the role of individual macronutrients to the diabetogenic process is not well delineated. We aimed to examine the impact of dietary fatty acid intake on fasting and 2-hour plasma glucose concentrations, as well as tissue-specific insulin action governing each. Normoglycemic controls (n = 15), athletes (n = 14), and obese (n = 23), as well as people with prediabetes (n = 10) and type 2 diabetes (n = 11), were queried about their habitual diet using a Food Frequency Questionnaire. All subjects were screened by an oral glucose tolerance test (OGTT) and studied using the hyperinsulinemic/euglycemic clamp with infusion of 6,62H2-glucose. Multiple regression was performed to examine relationships between dietary fat intake and 1) fasting plasma glucose, 2) % suppression of endogenous glucose production, 3) 2-hour post-OGTT plasma glucose, and 4) skeletal muscle insulin sensitivity (glucose rate of disappearance (Rd) and non-oxidative glucose disposal (NOGD)). The %kcal from saturated fat (SFA) was positively associated with fasting (β = 0.303, P = 0.018) and 2-hour plasma glucose (β = 0.415, P<0.001), and negatively related to % suppression of hepatic glucose production (β = -0.245, P = 0.049), clamp Rd (β = -0.256, P = 0.001) and NOGD (β = -0.257, P = 0.001). The %kcal from trans fat was also negatively related to clamp Rd (β = -0.209, P = 0.008) and NOGD (β = -0.210, P = 0.008). In contrast, the %kcal from polyunsaturated fat (PUFA) was negatively associated with 2-hour glucose levels (β = -0.383, P = 0.001), and positively related to Rd (β = 0.253, P = 0.007) and NOGD (β = 0.246, P = 0.008). Dietary advice to prevent diabetes should consider the underlying pathophysiology of the prediabetic state. PMID:26999667

  14. Dietary Fatty Acids Differentially Associate with Fasting Versus 2-Hour Glucose Homeostasis: Implications for The Management of Subtypes of Prediabetes

    Guess, Nicola; Perreault, Leigh; Kerege, Anna; Strauss, Allison; Bergman, Bryan C.

    2016-01-01

    Over-nutrition has fuelled the global epidemic of type 2 diabetes, but the role of individual macronutrients to the diabetogenic process is not well delineated. We aimed to examine the impact of dietary fatty acid intake on fasting and 2-hour plasma glucose concentrations, as well as tissue-specific insulin action governing each. Normoglycemic controls (n = 15), athletes (n = 14), and obese (n = 23), as well as people with prediabetes (n = 10) and type 2 diabetes (n = 11), were queried about their habitual diet using a Food Frequency Questionnaire. All subjects were screened by an oral glucose tolerance test (OGTT) and studied using the hyperinsulinemic/euglycemic clamp with infusion of 6,62H2-glucose. Multiple regression was performed to examine relationships between dietary fat intake and 1) fasting plasma glucose, 2) % suppression of endogenous glucose production, 3) 2-hour post-OGTT plasma glucose, and 4) skeletal muscle insulin sensitivity (glucose rate of disappearance (Rd) and non-oxidative glucose disposal (NOGD)). The %kcal from saturated fat (SFA) was positively associated with fasting (β = 0.303, P = 0.018) and 2-hour plasma glucose (β = 0.415, P<0.001), and negatively related to % suppression of hepatic glucose production (β = -0.245, P = 0.049), clamp Rd (β = -0.256, P = 0.001) and NOGD (β = -0.257, P = 0.001). The %kcal from trans fat was also negatively related to clamp Rd (β = -0.209, P = 0.008) and NOGD (β = -0.210, P = 0.008). In contrast, the %kcal from polyunsaturated fat (PUFA) was negatively associated with 2-hour glucose levels (β = -0.383, P = 0.001), and positively related to Rd (β = 0.253, P = 0.007) and NOGD (β = 0.246, P = 0.008). Dietary advice to prevent diabetes should consider the underlying pathophysiology of the prediabetic state. PMID:26999667

  15. Superselective Urokinase Infusion Therapy for Dorsalis Pedis Artery Occlusion in Buerger's Disease

    Occlusion of the proximal left dorsalis pedis artery (DPA) in a patient with Buerger's disease was treated by continuous urokinase intraarterial infusion using a microcatheter. Recanalization of the DPA and healing of a toe ulcer were achieved. The patient remains asymptomatic during a 4-year follow-up

  16. The urokinase receptor (uPAR) facilitates clearance of Borrelia burgdorferi

    J.W.R. Hovius; M.F. Bijlsma; G.J.W. van der Windt; W.J. Wiersinga; B.J.D. Boukens; J. Coumou; A. Oei; R. de Beer; A.F. de Vos; C. van 't Veer; A.P. van Dam; P. Wang; E. Fikrig; M.M. Levi; J.J.T.H. Roelofs; T. van der Poll

    2009-01-01

    The causative agent of Lyme borreliosis, the spirochete Borrelia burgdorferi, has been shown to induce expression of the urokinase receptor (uPAR); however, the role of uPAR in the immune response against Borrelia has never been investigated. uPAR not only acts as a proteinase receptor, but can also

  17. Tissue plasminogen activator and urokinase plasminogen activator in human epileptogenic pathologies

    A.M. Iyer; E. Zurolo; K. Boer; J.C. Baayen; F. Giangaspero; A. Arcella; G.C. Di Gennaro; V. Esposito; W.G.M. Spliet; P.C. van Rijen; D. Troost; J.A. Gorter; E. Aronica

    2010-01-01

    A growing body of evidence demonstrates the involvement of plasminogen activators (PAs) in a number of physiologic and pathologic events in the CNS. Induction of both tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA) has been observed in different experimental models of ep

  18. Regulation of urokinase receptors in monocytelike U937 cells by phorbol ester phorbol myristate acetate

    Picone, R; Kajtaniak, E L; Nielsen, L S;

    1989-01-01

    A specific surface receptor for urokinase plasminogen activator (uPA) recognizes the amino-terminal growth factor-like sequence of uPA, a region independent from and not required for the catalytic activity of this enzyme. The properties of the uPA receptor (uPAR) and the localization and distribu...

  19. The human receptor for urokinase plasminogen activator. NH2-terminal amino acid sequence and glycosylation variants

    Behrendt, N; Rønne, E; Ploug, M;

    1990-01-01

    The receptor for human urokinase-type plasminogen activator (u-PA) was purified from phorbol 12-myristate 13-acetate-stimulated U937 cells by temperature-induced phase separation of detergent extracts, followed by affinity chromatography with immobilized diisopropyl fluorophosphate-treated u-PA. ...

  20. The ligand-binding domain of the cell surface receptor for urokinase-type plasminogen activator

    Behrendt, N; Ploug, M; Patthy, L;

    1991-01-01

    The purified urokinase plasminogen activator receptor (u-PAR) was cleaved into two fragments by mild chymotrypsin treatment. The smaller fragment (apparent Mr 16,000) possessed the ligand-binding capability, as shown by chemical cross-linking analysis. This fragment constituted the NH2-terminal p...

  1. Characterization of human endothelial cell urokinase-type plasminogen activator receptor protein and messenger RNA

    Barnathan, E S; Kuo, A; Karikó, K;

    1990-01-01

    Human umbilical vein endothelial cells in culture (HUVEC) express receptors for urokinase-type plasminogen activators (u-PA). The immunochemical nature of this receptor and its relationship to u-PA receptors expressed by other cell types is unknown. Cross-linking active site-blocked u-PA to HUVEC...

  2. Competition Regime

    Danilo Icaza Ortiz

    2013-01-01

    This paper is a review of the competition regime works of various authors, published under the auspices of the University of the Hemispheres and the Corporation for Studies and Publications. Analyzes the structure, the general concepts, case law taken for development. Includes comments on the usefulness of this work for the study of competition law and the contribution to the lawyers who want to practice in this branch of economic law.

  3. Competition Regime

    Danilo Icaza Ortiz

    2013-01-01

    Full Text Available This paper is a review of the competition regime works of various authors, published under the auspices of the University of the Hemispheres and the Corporation for Studies and Publications. Analyzes the structure, the general concepts, case law taken for development. Includes comments on the usefulness of this work for the study of competition law and the contribution to the lawyers who want to practice in this branch of economic law.

  4. Cloning and expression of the receptor for human urokinase plasminogen activator, a central molecule in cell surface, plasmin dependent proteolysis

    Roldan, A L; Cubellis, M V; Masucci, M T;

    1990-01-01

    The surface receptor for urokinase plasminogen activator (uPAR) has been recognized in recent years as a key molecule in regulating plasminogen mediated extracellular proteolysis. Surface plasminogen activation controls the connections between cells, basement membrane and extracellular matrix, an...

  5. Urokinase-type plasminogen activator receptor as a predictor of poor outcome in patients with systemic inflammatory response syndrome

    Wu, Xiao-Ling; Long, Ding; Yu, Li; Yang, Jun-hui; Zhang, Yuan-chao; Geng, Feng

    2013-01-01

    BACKGROUND: Urokinase-type plasminogen activator (uPA) and urokinase-type plasminogen activator receptor (uPAR) are known as important factors, which mediate a variety of functions in terms of vascular homeostasis, inflammation and tissue repair. However, their role in systemic inflammatory response syndrome (SIRS) has been less well studied. This study aimed to test the hypothesis that the abnormalities of fibrinolysis and degradation of extracellular matrix mediated by uPA and uPAR are dire...

  6. Effect of urokinase on the proliferation of primary cultures of human prostatic cells

    The effects of exogenously added urokinase type plasminogen activator, tissue type plasminogen activator, plasmin and thrombin on the proliferation of primary cultures of cells derived from prostatic hyperplasia or prostatic carcinomas were investigated by measuring the incorporation of 3H-thymidine into the cultures. Addition of urokinase type plasminogen activator (1.35 x 10(-9) M) or thrombin (10(-7) M) to the culture medium caused a two-fold increase of 3H-thymidine incorporation, regardless of the origin of the prostatic cells. Tissue type plasminogen activator did not alter the rate of 3H-thymidine incorporation, whereas plasmin caused a 25% decrease of 3H-thymidine incorporation in all cultures

  7. Arterial thrombotic occlusions following percutaneous catheterization in infants and children: local thrombolytic therapy with urokinase

    Six cases of right external iliac and common femoral arterial thrombotic occlusions following transfemoral cardiac catheterization were found among the 117 infants and children with congenital heart disease. Physical findings of arterial thrombosis were lower extremity coldness and absence of femoral pulse, which appeared just after cardiac catheterization. Transcatheter local intrathrombotic infusion of Urokinase were done with 50000 IU bolus doses and 40000-50000 IU/h running doses. All 6 cases showed complete thrombolysis and re-establishment of femoral circulation. Three cases developed bleeding from arterial puncture sites, and were subsequently controlled properly by compression. No other complication was found. Our results suggest that local thrombolytic therapy with Urokinase is effective treatment method without significant complication for the infants and children with fresh thrombotic occlusions following cardiac catheterization and angiography

  8. Dictyostelium discoideum: a model for testing novel inhibitors of urokinase-type plasminogen activator

    Thompson, Elinor

    2013-01-01

    The social amoeba Dictyostelium discoideum is a useful non-animal eukaryote for testing novel compounds and dissecting cell regulatory molecular networks. We used this model organism to investigate the effect of a series of arylboronic acids and pinacol esters on development, chemotaxis and viability. These compounds were studied in parallel by collaborators for serine protease and urokinase-type plasminogen activator (uPA) inhibition, both in vitro and in vivo. In those biochemical assays, t...

  9. Rapid neural regulation of muscle urokinase-like plasminogen activator as defined by nerve crush.

    Hantaï, D; Rao, J. S.; Festoff, B W

    1990-01-01

    Muscle plasminogen activators (PAs), such as urokinase-like PA and, to a lesser extent, tissue PA, increase dramatically after denervation induced by axotomy. The PA/plasmin system has also been implicated in degradation of specific components of the muscle fiber basement membrane after local activation of plasminogen. These results suggest that neural regulation of muscle extracellular matrix metabolism accompanies or precedes regeneration after injury and is mediated by activation of PAs. I...

  10. Anterograde Intra-Arterial Urokinase Injection for Salvaging Fibular Free Flap

    Dae-Sung Lee

    2013-05-01

    Full Text Available We present a case of a 57-year-old male patient who presented with squamous cell carcinoma on his mouth floor with cervical and mandibular metastases. Wide glossectomy with intergonial mandibular ostectomy, and sequential reconstruction using fibular osteomyocutaneous free flap were planned. When the anastomosis between the peroneal artery of the fibular free flap and the right lingual artery was performed, no venous flow was observed at the vena comitans. Then re-anastomosis followed by topical application of papaverine and lidocaine was attempted. However, the blood supply was not recovered. Warm saline irrigation over 30 minutes was also useless. Microvascular thromboses of donor vessels were clinically suspected, so a solution of 100,000 units of urokinase was infused once through a 26-gauge angiocatheter inserted into the recipient artery just at the arterial anastomotic site, until the solution gushed out through the flap vena comitans. Immediately after the application of urokinase, arterial flow and venous return were restored. There were no complications during the follow-up period of 11 months. We believe that vibrating injuries from the reciprocating saw during osteotomies and flap insetting might be the cause of microvascular thromboses. The use of urokinase may provide a viable option for the treatment of suspicious intraoperative arterial thrombosis.

  11. Urokinase Separation from Cell Culture Broth of a Human Kidney Cell Line

    Vibha Bansal, Pradip K. Roychoudhury, Ashok Kumar

    2007-01-01

    Full Text Available A single step ion-exchange chromatography on a sulfo-propyl (SP- Sepharose column was performed to separate both the high molecular weight (HMW- and low molecular weight (LMW- forms of enzymatically active urokinase type plasminogen activator from human kidney (HT1080 cell culture media. The level of urokinase secreted by the cell line reached to about 145 Plough units/ml culture broth within 48 h of cultivation. The conditioned cell culture media was applied directly to the column without any prior concentration steps. Polyacrylamide gel electrophoresis of the column eluates in the presence of sodium dodecyl sulphate showed that the cell line secretes three forms of two-chain high molecular weight (HMW urokinase of molecular weights (Mr 64,000, 60,900 and 55,000. In addition, two low molecular weight (LMW forms of Mr 22,000 and 20,000; proteolytic cleavage products of HMW, were also found. The HMW and LMW forms had intrinsic plasminogen dependent proteolytic activity as judged by zymographic analysis. The specific activity of the pooled peak fractions increased (approximately 93-fold to values as high as 1481 Plough units/ mg protein. Both HMW as well as LMW forms were obtained in significantly high yields.

  12. Evaluation of intracoronary administration of urokinase and tirofiban in treating acute myocardial infarction with massive thrombus

    Objective: To assess the safety and efficacy of intracoronary administration of both urokinase and tirofiban for the treatment of acute myocardial infarction (AMI) with massive thrombus during emergency percutaneous coronary intervention in AMI patients. Methods: During the period of June 2008 June 2009 percutaneous coronary intervention was carried out in 85 patients with AMI, of whom seven patients showed high-burden thrombus in the infarct-related artery (IRA). Intracoronary infusion with urokinase and tirofiban was performed. During hospitalization and follow-up period the TIMI flow grade and myocardial perfusion were determined, side-effects of the drugs and major adverse cardiac events were observed, and cardiac color Doppler ultrasonography was employed for follow-up checkups. The clinical data were analyzed and therapeutic results were evaluated. Results: After stent deployment neither 'no-reflow' nor 'slow flow' phenomenon was observed and the grade of IRA flow was improved to TIMI 2-3 grade. During hospitalization no major adverse cardiac events such as death,re-infarction, target vessel revascularization, etc. occurred, and severe bleeding complications didn't occur either. During the follow-up period of 3-9 months, no major adverse cardiac events developed and the cardiac color Doppler ultrasonography showed that the left ventricle had an excellent performance in all cases. Conclusion: During emergency percutaneous coronary intervention for patients with AMI, intracoronary administration of both urokinase and tirofiban is safe and effective for AMI patients with massive thrombus. (authors)

  13. Flushing the liver with urokinase before transplantation does not prevent nonanastomotic biliary strictures.

    Pietersen, Lars C; den Dulk, A Claire; Braat, Andries E; Putter, Hein; Korkmaz, Kerem Sebib; Baranski, Andre G; Schaapherder, Alexander F M; Dubbeld, Jeroen; van Hoek, Bart; Ringers, Jan

    2016-04-01

    The aim of the present study was to assess whether flushing the donor liver with urokinase immediately before implantation reduces the incidence of nonanastomotic biliary strictures (NASs) after liver transplantation, without causing increased blood loss, analyzed as a historical cohort study. Between January 2005 and October 2012, all liver (re-)transplantations were included. Of the 185 liver transplant recipients included, 63 donor livers between January 2010 and October 2012 received urokinase (study group), whereas the donor liver of 122 consecutive recipients, who served as a historical control group, between January 2005 and January 2010 did not receive urokinase. Basic donor (Eurotransplant donor risk index) and recipient (age, body mass index, laboratory Model for End-Stage Liver Disease score) characteristics did not significantly differ in both groups. Thirty-three recipients developed NASs: 22 in the control group (18%) and 11 (17.5%) in the study group (P = 0.68). Analyzed separately for donation after circulatory death (P = 0.42) or donation after brain death (P = 0.89), there was no difference between the groups in incidence of NAS. Of all the recipients developing NAS, 7 (21%) needed retransplantation and all others were treated conservatively. Autologous blood transfusion requirements did not differ significantly between both groups (P = 0.91), whereas interestingly, more heterologous blood transfusions were needed in the control group (P blood loss. Liver Transplantation 22 420-426 2016 AASLD. PMID:26600096

  14. Urokinase perfusion prevents intrahepatic ischemic-type biliary lesion in donor livers

    Ren Lang; Qiang He; Zhong-Kui Jin; Dong-Dong Han; Da-Zhi Chen

    2009-01-01

    AIM: To evaluate whether urokinase perfusion of non-heart-beating cadaveric donor livers reduces the incidence of intrahepatic ischemic-type biliary lesions (IITBLs). METHODS: A prospective study was conducted to investigate potential microthrombosis in biliary microcirculation when non-heart-beating cadaveric livers were under warm or cold ischemic conditions. The experimental group included 140 patients who underwent liver transplantation during the period of January 2006 to December 2007, and survived for more than 1 year. The control group included 220 patients who received liver transplantation between July 1999 and December 2005 and survived for more than 1 year. In the experimental group, the arterial system of the donor liver was perfused twice with urokinase during cold perfusion and after trimming of the donor liver. The incidence of IITBLs was compared between the two groups. RESULTS: In the control group, the incidence of IITBLs was 5.9% (13/220 cases) after 3-11 mo of transplantation. In the experimental group, two recipients (1.4%) developed IITBLs at 3 and 6 mo after transplantation, respectively. The difference in the incidence between the two groups was statistically significant ( P < 0.05). CONCLUSION: Double perfusion of cadaveric livers from non-heart-beating donors with urokinase may reduce the incidence of IITBLs.

  15. Analysis of multi-factors affecting symptomatic intracranial hemorrhage in intraarterial thrombolysis with urokinase for acute ischemic stroke

    Objective: To explore the causes and preventive measures of symptomatic intracranial hemorrhage in 217 patients with acute cerebral ischemic stroke treated with local intra-arterial urokinase. Methods: From February 1999 to June 2004, 217 patients were treated for acute ischemic stroke with local intra-arterial urokinase in our hospital. Factors associated with symptomatic intracranial hemorrhage of intra-arterial thrombolysis were analyzed by Stepwise logistic regression to identify some factors relating the prediction symptomatic intracranial hemorrhage. Results: Symptomatic intracranial hemorrhage occurred in 8 cases (3.7%). Predictors of the symptomatic intracranial hemorrhage were the elevated systolic blood pressure before therapy (odds ratio, 1.096; 95% CI, 1.006 to 1.194) and urokinase (UK) treatment (odds ratio, 1.068 ; 95% CL, 1.053 to 1.247). Risk of secondary symptomatic intracranial hemorrhage was increased with elevated systolic blood pressure. Other factors like age, initial treating time, NIHSS, diabetes and collateral circulation did not predict the symptomatic intracranial hemorrhage respectively. Conclusions: Predictors of symptomatic intracranial hemorrhage after local intra-arterial infusion of urokinase for acute ischemic stroke were the elevated systolic blood pressure before therapy and urokinase (UK) treatment. (authors)

  16. Correlation of 2 hour, 4 hour, 8 hour and 12 hour urine protein with 24 hour urinary protein in preeclampsia.

    Savita Rani Singhal

    2014-09-01

    Full Text Available To find shortest and reliable time period of urine collection for determination of proteinuria.It is a prospective study carried out on 125 pregnant women with preeclampsia after 20 weeks of gestation having urine albumin >1 using dipstick test. Urine was collected in five different time intervals in colors labeled containers with the assistance of nursing staff; the total collection time was 24 hours. Total urine protein of two-hour, four-hour, eight-hour, 12-hour and 24-hour urine was measured and compared with 24-hour collection. Data was analyzed using the Pearson correlation coefficient.There was significant correlation (p value < 0.01 in two, four, eight and 12-hour urine protein with 24-urine protein, with correlation coefficient of 0.97, 0.97, 0.96 and 0.97, respectively. When a cut off value of 25 mg, 50 mg. 100 mg, and 150 mg for urine protein were used for 2-hour, 4-hours, 8-hour and 12-hour urine collection, a sensitivity of 92.45%, 95.28%, 91.51%, and 96.23% and a specificity of 68.42%, 94.74%, 84.21% and 84.21% were obtained, respectively.Two-hour urine proteins can be used for assessment of proteinuria in preeclampsia instead of gold standard 24-hour urine collection for early diagnosis and better patient compliance.

  17. Interconversion of Active and Inactive Conformations of Urokinase-Type Plasminogen Activator

    Liu, Zhuo; Kromann-Hansen, Tobias; Lund, Ida K;

    2012-01-01

    The catalytic activity of serine proteases depends on a salt-bridge between the amino group of residue 16 and the side chain of Asp194. The salt-bridge stabilizes the oxyanion hole and the S1 specificity pocket of the protease. Some serine proteases exist in only partially active forms, in which...... the amino group of residue 16 is exposed to the solvent. Such a partially active state is assumed by a truncated form of the murine urokinase-type plasminogen activator (muPA), consisting of residues 16-243. Here we investigated the allosteric interconversion between partially active states and the...

  18. Urokinase-type plasminogen activator receptor (uPAR) as a promising new imaging target

    Persson, Morten; Kjaer, Andreas

    2013-01-01

    Urokinase-type plasminogen activator receptor (uPAR) has been shown to be of special importance during cancer invasion and metastasis. However, currently, tissue samples are needed for measurement of uPAR expression limiting the potential as a clinical routine. Therefore, non-invasive methods are...... the cell surface. Due to the importance of uPAR in cancer invasion and metastasis, a number of high-affinity ligands have been identified during the last decades. These ligands have recently been used as starting point for the development of a number of ligands for imaging of uPAR using various...

  19. Targeting the autolysis loop of urokinase-type plasminogen activator with conformation-specific monoclonal antibodies

    Bøtkjær, Kenneth Alrø; Fogh, Sarah; Bekes, Erin C;

    2011-01-01

    Tight regulation of serine proteases is essential for their physiological function, and unbalanced states of protease activity have been implicated in a variety of human diseases. One key example is the presence of uPA (urokinase-type plasminogen activator) in different human cancer types...... to harbour the epitopes for three conformation-specific monoclonal antibodies, two with a preference for the zymogen form pro-uPA, and one with a preference for active uPA. All three antibodies were shown to have overlapping epitopes, with three common residues being crucial for all three antibodies...

  20. Characterization of human endothelial cell urokinase-type plasminogen activator receptor protein and messenger RNA

    Barnathan, E S; Kuo, A; Karikó, K; Rosenfeld, L; Murray, S C; Behrendt, N; Rønne, E; Weiner, D; Henkin, J; Cines, D B

    1990-01-01

    Human umbilical vein endothelial cells in culture (HUVEC) express receptors for urokinase-type plasminogen activators (u-PA). The immunochemical nature of this receptor and its relationship to u-PA receptors expressed by other cell types is unknown. Cross-linking active site-blocked u-PA to HUVEC...... endothelial cell cDNA library using the polymerase chain reaction (PCR) and oligonucleotide primers corresponding to the DNA sequence of the receptor cloned from transformed human fibroblasts (Roldan et al, EMBO J 9:467, 1990). The size of the cDNA (approximately 1,054 base pairs, bp) and the presence of a...

  1. Urokinase Expression by Tumor Suppressor Protein p53: A Novel Role in mRNA Turnover

    Shetty, Praveenkumar; Velusamy, Thirunavukkarasu; Bhandary, Yashodhar P.; Shetty, Rashmi S.; Liu, Ming-Cheh; Shetty, Sreerama

    2008-01-01

    Lung carcinoma (H1299) cells deficient in p53 (p53−/−) express large amounts of urokinase-type plasminogen activator (uPA) protein and uPA mRNA, and exhibit slower degradation of uPA mRNA than that of p53-expressing nonmalignant Beas2B human airway epithelial cells. Expression of p53 protein in H1299 cells, upon transfection with p53 cDNA, suppressed basal as well as uPA-induced expression of uPA protein in both conditioned media and cell lysates, and decreased the level of steady-state uPA m...

  2. Soluble Urokinase Plasminogen Activator Receptor Levels in Tuberculosis Patients at High Risk for Multidrug Resistance

    Tri Yudani Mardining Raras; Triwahju Astuti; Iin Noor Chozin

    2012-01-01

    The soluble urokinase plasminogen activator receptor (suPAR) has been shown to be a strong prognostic biomarker for tuberculosis (TB). In the present study, the profiles of plasma suPAR levels in pulmonary TB patients at high risk for multidrug resistance were analyzed and compared with those in multidrug resistant (MDR)-TB patients. Forty patients were prospectively included, consisting of 10 MDR-TB patients and 30 TB patients at high risk for MDR, underwent clinical assesment. Plasma suPAR ...

  3. Synthesis of New Ligands of Urokinase Receptor and New Urotensin-II Derivatives

    Yousif, Ali Munaim

    2015-01-01

    Unit 1: The urokinase-type plasminogen activator receptor (uPAR) is involved in the regulation of cell migration. The uPAR is formed by three domains connected by short linker region. The Ser88-Arg-Ser- Arg-Tyr92 is the minimum chemotactic sequence of uPAR required to induce the same intracellular signaling as result of its binding with formyl peptide receptor (FPRs). With aim to perform a SAR study on this sequence we have synthesized a large peptide library using phosphorylated ...

  4. Combination of irradiation, carcinostatics, and urokinase for the treatment of uterine cervical carcinoma

    In the treatment of carcinoma of the cervix uteri, histological changes were studied by dividing patients into 2 groups. One group was treated with radio-therapy and the other was treated with radiotherapy combined with chemotherapy, using antineoplastic agents which were used alone or in combination with urokinase. In regard to radiotherapy alone, Linac 10 MeV x-ray, 60Co-teletherapy, and radium contiguous irradiation were evaluated, and marked changes in cancerous parenchyma were seen with irradiation over 3000 rad. Irradiation of 3000 rad caused destruction of cancer cells, separation of the cells, inclusion of the focus, necrosis of hyalinization, and infiltration of neutrophils and lymphocytes. Approximately 4000 rad caused hyperplasia of the interstitial connective tissues, in addition to the above-mentioned changes. Approximately 5000 rad caused destruction and disappearance of cancer tissues, and approximately 6000 rad caused disappearance of the cancer cells which were then only connective or necrostic tissues. When radiotherapy was combined with BLM and FAMT therapies, degenerative changes were observed in many cases with irradiation of 1200 - 2400 mgh of radium or 1000 - 3000 rad of Linac or 60Co. The addition of urokinase tended to further accelerate the degeneration begun by BLM therapy. (Tsunoda, M.)

  5. Urokinase receptor cleavage correlates with tumor volume in a transgenic mouse model of breast cancer

    Thurison, Tine; Almholt, Kasper; Gårdsvoll, Henrik;

    2015-01-01

    The urokinase plasminogen activator system plays a key role in tissue degradation during cancer invasion. The linker region between domains I and II of the intact, three domain urokinase receptor uPAR(I-III) is highly susceptible to proteolytic cleavage and the resulting cleaved uPAR forms are...... strong prognostic biomarkers in several types of cancer, i.e., high levels of the cleaved uPAR forms indicate poor survival. To better understand the role of uPAR cleavage in cancer, we have designed immunoassays for specific quantification of intact mouse uPAR [muPAR(I-III)] and mouse uPAR domain I [mu......PAR(I)]. The level of muPAR(I) is significantly increased in mammary tumor-bearing mice compared to controls and, notably, there is a strong correlation to tumor volume. In contrast, the tumor volume is only weakly correlated to the level of intact muPAR(I-III), indicating that cleavage of muPAR is a more...

  6. The liberated domain I of urokinase plasminogen activator receptor--a new tumour marker in small cell lung cancer

    Almasi, Charlotte E; Drivsholm, Lars; Pappot, Helle;

    2013-01-01

    The prognosis of small cell lung cancer (SCLC) remains poor with a 5-year survival rate of 4-6%. In non-small cell lung cancer (NSCLC), high levels of intact and cleaved forms of the receptor for urokinase plasminogen activator (uPAR) are significantly associated with short overall survival. Our...

  7. The Heparin-Induced Thrombocytopenia and Thrombosis Syndrome: Treatment with Intraarterial Urokinase and Systemic Platelet Aggregation Inhibitors

    We report a case of the heparin-induced thrombocytopenia and thrombosis syndrome presenting with acute ischemia of a lower limb. The patient was successfully treated by withdrawal of heparin products, intraarterial urokinase, and platelet anti-aggregation therapy consisting of Dextran and aspirin

  8. Plasminogen activation by receptor-bound urokinase. A kinetic study with both cell-associated and isolated receptor

    Ellis, V; Behrendt, N; Danø, K

    1991-01-01

    The specific cellular receptor for urokinase-type plasminogen activator (uPA) is found on a variety of cell types and has been postulated to play a central role in the mediation of pericellular proteolytic activity. We have studied the kinetics of plasminogen (Plg) activation catalyzed by uPA spe...

  9. Soluble urokinase receptor levels in plasma during 5 years of highly active antiretroviral therapy in HIV-1-infected patients

    Ostrowski, Sisse R; Katzenstein, Terese L; Piironen, Timo;

    2004-01-01

    High blood levels of the soluble urokinase receptor (suPAR) strongly predict increased mortality in human immunodeficiency virus-1 (HIV-1)-infected patients. This study investigated the plasma concentration of suPAR in 29 treatment-naive HIV-1-infected patients during 5 years treatment with highl...

  10. Expression of urokinase plasminogen activator, its receptor and type-1 inhibitor in malignant and benign prostate tissue

    Usher, Pernille Autzen; Thomsen, Ole Frøkjær; Iversen, Peter; Johnsen, Morten; Brünner, Nils; Høyer-Hansen, Gunilla; Andreasen, Peter; Danø, Keld; Nielsen, Boye Schnack

    2005-01-01

    The plasminogen activation (PA) cascade participates in degradation of extracellular matrix during cancer invasion. We have studied the expression of urokinase-type plasminogen activator (uPA) mRNA, uPA receptor (uPAR) mRNA and immunoreactivity, and type-1 plasminogen activator inhibitor (PAI-1) ...

  11. The plasma level of soluble urokinase receptor is elevated in patients with Streptococcus pneumoniae bacteraemia and predicts mortality

    Wittenhagen, p; Kronborg, Gitte; Nielsen, H; Obel, N; Pedersen, SS; Eugen-Olsen, Jesper; Weis, Nina Margrethe

    2004-01-01

    This multicentre prospective study was conducted to investigate whether the level of the soluble form of urokinase-type plasminogen activator receptor (suPAR) is elevated during pneumococcal bacteraemia and is of predictive value in the early stage of the disease. Plasma levels of suPAR were incr...

  12. Cellular receptor for urokinase plasminogen activator. Carboxyl-terminal processing and membrane anchoring by glycosyl-phosphatidylinositol

    Ploug, M; Rønne, E; Behrendt, N;

    1991-01-01

    The cellular receptor for human urokinase-type plasminogen activator (u-PAR) is shown by several independent criteria to be a true member of a family of integral membrane proteins, anchored to the plasma membrane exclusively by a COOH-terminal glycosyl-phosphatidylinositol moiety. 1) Amino acid a...

  13. A conserved TATA-less proximal promoter drives basal transcription from the urokinase-type plasminogen activator receptor gene

    Soravia, E; Grebe, A; De Luca, P;

    1995-01-01

    The urokinase-type plasminogen activator receptor (uPAR) focuses at the cell surface the activation of pro-uPA and, hence, the formation of plasmin, thus enhancing directional extracellular proteolysis. To characterize the transcriptional regulatory mechanisms that control receptor expression, we...

  14. Aberrant glomerular filtration of urokinase-plasminogen activator in nephrotic syndrome leads to amiloride-sensitive plasminogen activation in urine

    Stæhr, Mette; Buhl, Kristian Bergholt; Andersen, René F;

    2015-01-01

    In nephrotic syndrome, aberrant glomerular filtration of plasminogen and conversion to active plasmin in pre-urine is thought to activate proteolytically ENaC and contribute to sodium retention and edema. The ENaC blocker amiloride is an off-target inhibitor of urokinase-type plasminogen activator...

  15. Genetic association of urokinase-type plasminogen activator gene rs2227564 site polymorphism with sporadic Alzheimer's disease in the Han Chinese population

    Xuelian Ji; Longfei Jia; Jianping Jia; Li Qi

    2012-01-01

    A missense C/T polymorphism in exon 6 (the NCBI rsID is rs2227564) of the urokinase-type plasminogen activator gene has been identified as a possible hot spot for Alzheimer's disease risk.The present study analyzed urokinase-type plasminogen gene polymorphisms of rs2227564 with sporadic Alzheimer's disease by PCR-restriction fragment length polymorphism.Results showed that CC,CT and TT genotype distribution frequencies had significant differences between sporadic Alzheimer's disease patients and healthy controls.In-depth analysis of the association between urokinase-type plasminogen gene rs2227564 polymorphisms and sporadic Alzheimer's disease indicated that people with the C-positive genotype CC + CT were at a higher risk for developing sporadic Alzheimer's disease.These results support the contribution of the polymorphisms of rs2227564 in the urokinase-type plasminogen gene to the pathogenesis of sporadic Alzheimer's disease in the Han Chinese population.

  16. Urokinase plasminogen activator receptor affects bone homeostasis by regulating osteoblast and osteoclast function

    Furlan, Federico; Galbiati, Clara; Jørgensen, Niklas R;

    2007-01-01

    reorganization in mature osteoclasts. INTRODUCTION: Urokinase receptor (uPAR) is actively involved in the regulation of important cell functions, such as proliferation, adhesion, and migration. It was previously shown that the major players in bone remodeling, osteoblasts and osteoclasts, express uPAR and...... to mechanical tests. UPAR KO calvaria osteoblasts were characterized by proliferation assays, RT-PCR for important proteins secreted during differentiation, and immunoblot for activator protein 1 (AP-1) family members. In vitro osteoclast formation was tested with uPAR KO bone marrow monocytes in the...... osteoblasts showed a proliferative advantage with no difference in apoptosis, higher matrix mineralization, and earlier appearance of alkaline phosphatase (ALP). Surface RANKL expression at different stages of differentiation was not altered. AP-1 components, such as JunB and Fra-1, were upregulated in u...

  17. Urokinase mediates endothelial cell survival via induction of the X-linked inhibitor of apoptosis protein

    Prager, Gerald W; Mihaly, Judit; Brunner, Patrick M;

    2008-01-01

    factor kappaB (NF-kappaB) p52 activation. Indeed, blocking NF-kappaB activation by using specific NF-kappaB inhibitors abolished uPA-induced cell survival as it blocked uPA-induced XIAP up-regulation. Furthermore, down-regulating XIAP expression by small interfering RNA (siRNA) significantly reduced u......Urokinase-type plasminogen activator (uPA) additionally elicits a whole array of pro-angiogenic responses, such as differentiation, proliferation, and migration. In this study, we demonstrate that in endothelial cells uPA also protects against apoptosis by transcriptional up-regulation and......PA-dependent endothelial cell survival. This mechanism is also important for VEGF-induced antiapoptosis because VEGF-dependent up-regulation of XIAP was found defective in uPA(-/-) endothelial cells. This led us to conclude that uPA is part of a novel NF-kappaB-dependent cell survival pathway....

  18. Effects of urokinase-type plasminogen activator in the acquisition, expression and reinstatement of cocaine-induced conditioned-place preference

    Bahi, Amine; Kusnecov, Alexander W; Dreyer, Jean-Luc

    2008-01-01

    Cocaine and many other psychostimulants strongly induce urokinase-type plasminogen activator (uPA) expression in the mesolimbic dopaminergic pathway, which plays a major role in drug-mediated behavioral plasticity [Bahi A, Boyer F, Gumy C, Kafri T, Dreyer JL. In vivo gene delivery of urokinase-type plasminogen activator with regulatable lentivirus induces behavioral changes in chronic cocaine administration. Eur J Neurosci 2004;20:3473–88; Bahi A, Boyer F, Kafri T, Dreyer JL. Silencing urokin...

  19. The Prognostic Significance of Soluble Urokinase Plasminogen Activator Receptor in Acute Myeloid Leukemia

    Nergiz Erkut

    2016-05-01

    Full Text Available Objective: The soluble urokinase plasminogen activator receptor (suPAR is a soluble form of the urokinase plasminogen activator receptor expressed in various immune and cancer cells. The levels of suPAR have been demonstrated to correlate with prognosis in various cancers. This study was intended to investigate serum suPAR levels and their effect on prognosis in patients with acute myeloid leukemia (AML. Materials and Methods: Thirty newly diagnosed patients with AML and 29 healthy individuals were enrolled. Serum suPAR levels were analyzed by enzyme-linked immunosorbent assay. Results: Serum suPAR levels were significantly higher in patients with AML than in healthy individuals (9±5.9 ng/mL and 2.4±1.4 ng/mL, respectively; p<0.001. Positive correlation was determined between suPAR levels and white blood cell counts (p<0.01. Serum suPAR levels were lower in patients who achieved complete response than in patients not achieving complete response (5.5±2.2 ng/mL and 12±6.6 ng/mL, respectively; p<0.001. The median overall survival was longer in patients with serum suPAR levels below 6.71 ng/mL than in those with serum suPAR levels above 6.71 ng/mL (12.6±13.2 months and 1.71±0.6 months, respectively; p=0.02. Multivariate Cox regression analysis showed that suPAR had independent prognostic value (95% confidence interval: 1.029-6.259; p<0.05 in AML. Conclusion: Serum suPAR levels can be used as a prognostic marker in AML.

  20. Activation of human pro-urokinase by unrelated proteases secreted by Pseudomonas aeruginosa.

    Beaufort, Nathalie; Seweryn, Paulina; de Bentzmann, Sophie; Tang, Aihua; Kellermann, Josef; Grebenchtchikov, Nicolai; Schmitt, Manfred; Sommerhoff, Christian P; Pidard, Dominique; Magdolen, Viktor

    2010-06-15

    Pathogenic bacteria, including Pseudomonas aeruginosa, interact with and engage the host plasminogen (Plg) activation system, which encompasses the urokinase (uPA)-type Plg activator, and is involved in extracellular proteolysis, including matrilysis and fibrinolysis. We hypothesized that secreted bacterial proteases might contribute to the activation of this major extracellular proteolytic system, thereby participating in bacterial dissemination. We report that LasB, a thermolysin-like metalloprotease secreted by Ps. aeruginosa, converts the human uPA zymogen into its active form (kcat=4.9 s-1, Km=8.9 microM). Accordingly, whereas the extracellular secretome from the LasB-expressing pseudomonal strain PAO1 efficiently activates pro-uPA, the secretome from the isogenic LasB-deficient strain PDO240 is markedly less potent in pro-uPA activation. Still, both secretomes induce some metalloprotease-independent activation of the human zymogen. The latter involves a serine protease, which we identified via both recombinant protein expression in Escherichia coli and purification from pseudomonal cultures as protease IV (PIV; kcat=0.73 s-1, Km=6.2 microM). In contrast, neither secretomes nor the pure proteases activate Plg. Along with this, LasB converts Plg into mini-Plg and angiostatin, whereas, as reported previously, it processes the uPA receptor, inactivates the plasminogen activator inhibitor 1, and activates pro-matrix metalloproteinase 2. PIV does not target these factors at all. To conclude, LasB and PIV, although belonging to different protease families and displaying quite different substrate specificities, both activate the urokinase-type precursor of the Plg activation cascade. Direct pro-uPA activation, as also reported for other bacterial proteases, might be a frequent phenomenon that contributes to bacterial virulence. PMID:20337595

  1. The receptor for urokinase regulates TLR2 mediated inflammatory responses in neutrophils.

    Gang Liu

    Full Text Available The urokinase-type plasminogen activator receptor (uPAR, a glycosylphosphatidylinositol (GPI anchored membrane protein, regulates urokinase (uPA protease activity, chemotaxis, cell-cell interactions, and phagocytosis of apoptotic cells. uPAR expression is increased in cytokine or bacteria activated cell populations, including macrophages and monocytes. However, it is unclear if uPAR has direct involvement in the response of inflammatory cells, such as neutrophils and macrophages, to Toll like receptor (TLR stimulation. In this study, we found that uPAR is required for optimal neutrophil activation after TLR2, but not TLR4 stimulation. We found that the expression of TNF-α and IL-6 induced by TLR2 engagement in uPAR-/- neutrophils was less than that in uPAR+/+ (WT neutrophils. Pretreatment of neutrophils with PI-PLC, which cleaves GPI moieties, significantly decreased TLR2 induced expression of TNF-α in WT neutrophils, but demonstrated only marginal effects on TNF-α expression in PAM treated uPAR-/- neutrophils. IκB-α degradation and NF-κB activation were not different in uPAR-/- or WT neutrophils after TLR2 stimulation. However, uPAR is required for optimal p38 MAPK activation after TLR2 engagement. Consistent with the in vitro findings that uPAR modulates TLR2 engagement induced neutrophil activation, we found that pulmonary and systemic inflammation induced by TLR2, but not TLR4 stimulation is reduced in uPAR-/- mice compared to WT counterparts. Therefore, our data suggest that neutrophil associated uPAR could be a potential target for treating acute inflammation, sepsis, and organ injury related to severe bacterial and other microbial infections in which TLR2 engagement plays a major role.

  2. The superoxide scavenger TEMPOL induces urokinase receptor (uPAR expression in human prostate cancer cells

    Francis Joseph

    2006-06-01

    Full Text Available Abstract There is little understanding of the effect that reactive oxygen metabolites have on cellular behavior during the processes of invasion and metastasis. These oxygen metabolites could interact with a number of targets modulating their function such as enzymes involved in basement membrane dissolution, adhesion molecules involved in motility or receptors involved in proliferation. We investigated the effect of increased scavenging of superoxide anions on the expression of the urokinase receptor (uPAR in PC-3M human prostate cancer cells. Urokinase receptor is a GPI-linked cell surface molecule which mediates multiple functions including adhesion, proliferation and pericellular proteolysis. Addition of the superoxide scavenger 4-hydroxy-2,2,6,6-tetramethylpiperidinyloxy (TEMPOL to PC-3M cultures stimulated expression of uPAR protein peaking between 48 and 72 hours. Cell surface expression of the uPAR was also increased. Surprisingly, uPAR transcript levels increased only slightly and this mild increase did not coincide with the striking degree of protein increase. This disparity indicates that the TEMPOL effect on uPAR occurs through a post-transcriptional mechanism. TEMPOL presence in PC-3M cultures reduced intracellular superoxide-type species by 75% as assayed by NBT dye conversion; however this reduction significantly diminished within hours following TEMPOL removal. The time gap between TEMPOL treatment and peak uPAR protein expression suggests that reduction of reactive oxygen metabolites in prostate cancer cells initiates a multistep pathway which requires several hours to culminate in uPAR induction. These findings reveal a novel pathway for uPAR regulation involving reactive oxygens such as superoxide anion.

  3. Activity and expression of urokinase-type plasminogen activator and matrix metalloproteinases in human colorectal cancer

    Matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), and urokinase-type plasminogen activator (uPA) are involved in colorectal cancer invasion and metastasis. There is still debate whether the activity of MMP-2 and MMP-9 differs between tumors located in the colon and rectum. We designed this study to determine any differences in the expression of MMP-2, MMP-9 and uPA system between colon and rectal cancer tissues. Cancer tissue samples were obtained from colon carcinoma (n = 12) and rectal carcinomas (n = 10). MMP-2 and MMP-9 levels were examined using gelatin zymography and Western blotting; their endogenous inhibitors, tissue inhibitor of metalloproteinase-2 (TIMP-2) and tissue inhibitor of metalloproteinase-1 (TIMP-1), were assessed by Western blotting. uPA, uPAR and PAI-1 were examined using enzyme-linked immunosorbent assay (ELISA). The activity of uPA was assessed by casein-plasminogen zymography. In both colon and rectal tumors, MMP-2, MMP-9 and TIMP-1 protein levels were higher than in corresponding paired normal mucosa, while TIMP-2 level in tumors was significantly lower than in normal mucosa. The enzyme activities or protein levels of MMP-2, MMP-9 and their endogenous inhibitors did not reach a statistically significant difference between colon and rectal cancer compared with their normal mucosa. In rectal tumors, there was an increased activity of uPA compared with the activity in colon tumors (P = 0.0266), however urokinase-type plasminogen activator receptor (uPAR) and plasminogen activator inhibitor-1 (PAI-1) showed no significant difference between colon and rectal cancer tissues. These findings suggest that uPA may be expressed differentially in colon and rectal cancers, however, the activities or protein levels of MMP-2, MMP-9, TIMP-1, TIMP-2, PAI-1 and uPAR are not affected by tumor location in the colon or the rectum

  4. Outcome evaluation of intra-arterial infusion of urokinase for acute ischemic stroke

    To evaluate the results of intra-arterial urokinase thrombolysis in cases of acute ischemic stroke and to define the factors affecting prognosis. Forty-eight patients with angiographically proven occlusion of the intracranial arteries were treated with local intra-arterial infusion of urokinase within six hours of the onset of symptoms. Neurologic status was evaluated on admission and on discharge using the NIH (National Institute of Health) stroke scale score (SSS). When the SSS decreased by at least four points, this was considered indicative of an improved clinical outcome. Complete recanalization was achieved in 17/48 patients (35%), including 8 of 13 (62%) with occlusion of the vertebrobasilar artery (VBA), 9 of 20 (45%) with occlusion of the middle cerebral artery (MCA), and none of 15 with occlusion of the internal carotid artery (ICA). Neurologic status improved in 12 (60%) of patients with MCA occlusion, in five (38%) of those with VBA occlusion and in three (20%) of those with ICA occlusion (p less than 0.005). Patients in whom occluded MCA was completely recanalized showed greater clinical improvement than those with partial or no recanalization (p less than 0.05). The overall mortality rate was 21%, 43% (9/21) in patients in whom CT revealed signs of early infarct, but only 4% (1/27) in those without this sign (p less than 0.05). The mortality rate of patients with parenchymal hematoma (4/5) was higher than that of those with hemorrhagic infarct (3/9) or without hemorrhage (3/34) (p less than 0.005). In patients in whom occluded MCA was completely recanalized, the clinical outcome was better, while patients with VBA occlusion did not benefit from recanalization. The presence on CT scans of signs of early infarct and of parenchymal hematoma after thrombolysis correlated with a high mortality rate. (author)

  5. Outcome evaluation of intra-arterial infusion of urokinase for acute ischemic stroke

    Shi, Hai Bin [First Affiliated Hospital of Nanjing Medical University, Nanjing (China); Suh, Dae Chul; Lim, Soo Mee [Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of); And Others

    2000-06-01

    To evaluate the results of intra-arterial urokinase thrombolysis in cases of acute ischemic stroke and to define the factors affecting prognosis. Forty-eight patients with angiographically proven occlusion of the intracranial arteries were treated with local intra-arterial infusion of urokinase within six hours of the onset of symptoms. Neurologic status was evaluated on admission and on discharge using the NIH (National Institute of Health) stroke scale score (SSS). When the SSS decreased by at least four points, this was considered indicative of an improved clinical outcome. Complete recanalization was achieved in 17/48 patients (35%), including 8 of 13 (62%) with occlusion of the vertebrobasilar artery (VBA), 9 of 20 (45%) with occlusion of the middle cerebral artery (MCA), and none of 15 with occlusion of the internal carotid artery (ICA). Neurologic status improved in 12 (60%) of patients with MCA occlusion, in five (38%) of those with VBA occlusion and in three (20%) of those with ICA occlusion (p less than 0.005). Patients in whom occluded MCA was completely recanalized showed greater clinical improvement than those with partial or no recanalization (p less than 0.05). The overall mortality rate was 21%, 43% (9/21) in patients in whom CT revealed signs of early infarct, but only 4% (1/27) in those without this sign (p less than 0.05). The mortality rate of patients with parenchymal hematoma (4/5) was higher than that of those with hemorrhagic infarct (3/9) or without hemorrhage (3/34) (p less than 0.005). In patients in whom occluded MCA was completely recanalized, the clinical outcome was better, while patients with VBA occlusion did not benefit from recanalization. The presence on CT scans of signs of early infarct and of parenchymal hematoma after thrombolysis correlated with a high mortality rate. (author)

  6. Interleukin-6 infusion during human endotoxaemia inhibits in vitro release of the urokinase receptor from peripheral blood mononuclear cells

    Ostrowski, S R; Plomgaard, P; Fischer, C P;

    2005-01-01

    Leucocyte expression of the urokinase receptor [urokinase-type plasminogen activator receptor (uPAR)] is regulated by inflammatory mediators. This study investigated the in vivo effect of endotoxin, interleukin (IL)-6 and tumour necrosis factor (TNF)-alpha on uPAR-release in vivo and in vitro in...... humans. Healthy subjects received intravenous endotoxin injection [high-dose, 2 ng/kg (n=8) and low-dose, 0.06 ng/kg (n=7)], coadministration of 0.06 ng/kg endotoxin and 3 h recombinant human (rh)IL-6 infusion (n=7) or 3 h infusion of rhIL-6 (n=6), rhTNF-alpha (n=6) or NaCl (n=5). Soluble uPAR (su...

  7. Quantitative PET of human urokinase-type plasminogen activator receptor with 64Cu-DOTA-AE105

    Persson, Morten; Madsen, Jacob; Østergaard, Søren;

    2012-01-01

    Expression levels of the urokinase-type plasminogen activator receptor (uPAR) represent an established biomarker for poor prognosis in a variety of human cancers. The objective of the present study was to explore whether noninvasive PET can be used to perform a quantitative assessment of expressi...... levels of uPAR across different human cancer xenograft models in mice and to illustrate the clinical potential of uPAR PET in future settings for individualized therapy.......Expression levels of the urokinase-type plasminogen activator receptor (uPAR) represent an established biomarker for poor prognosis in a variety of human cancers. The objective of the present study was to explore whether noninvasive PET can be used to perform a quantitative assessment of expression...

  8. In vivo gene delivery of urokinase-type plasminogen activator with regulatable lentivirus induces behavioural changes in chronic cocaine administration

    Bahi, Amine; Boyer, Frederic; Gumy, Christèle; Kafri, Tal; Dreyer, Jean-Luc

    2005-01-01

    Serine proteases play a key function in extracellular processes affecting central nervous system plasticity. Recently, the role of extracellular proteolytic processes in regulating synaptic structure and function has been described. However, to date direct evidence linking extracellular serine protease activity with drug-related behavioural changes has not been documented. Importantly, in a screening for genes induced after drug treatment we found that urokinase plasminogen-type activator (uP...

  9. Localization of urokinase-type plasminogen activator in stromal cells in adenocarcinomas of the colon in humans.

    Grøndahl-Hansen, J.; Ralfkiaer, E; Kirkeby, L. T.; P. Kristensen; Lund, L. R.; Danø, K

    1991-01-01

    Human colon adenocarcinomas and adjacent normal colon tissues were stained immunohistochemically with three different monoclonal antibodies and one preparation of polyclonal antibodies against each of the two plasminogen activators, uPA (urokinase type) and tPA (tissue type). The staining patterns seen with the respective sets of antibodies were identical. In all of 10 cases, staining for uPA in the normal colon tissue was confined to scattered fibroblastlike cells in the lamina propria. Othe...

  10. Urokinase-targeted recombinant bacterial protein toxins-a rationally designed and engineered anticancer agent for cancer therapy

    Yizhen LIU; Shi-Yan LI

    2009-01-01

    Urokinase-targeted recombinant bacterial protein toxins are a sort of rationally designed and engineered anticancer recombinant fusion proteins representing a novel class of agents for cancer therapy.Bacterial protein toxins have long been known as the primary virulence factor(s) for a variety of pathogenic bacteria and are the most powerful human poisons.On the other hand,it has been well documented that urokinase-type plasminogen activator (uPA) and urokinase plasminogen activator receptor (uPAR),making up the uPA system,are overexpressed in a variety of human tumors and tumor cell lines.The expression of uPA system is highly correlated with tumor invasion and metastasis.To exploit these characteristics in the design of tumor cell-selective cytotoxins,two prominent bacterial protein toxins,i.e.,the diphtheria toxin and anthrax toxin are deliberately engineered through placing a sequence targeted specifically by the uPA system to form anticancer recombinant fusion proteins.These uPA system-targeted bacterial protein toxins are activated selectively on the surface of uPA systemexpressing tumor cells,thereby killing these cells.This article provides a review on the latest progress in the exploitation of these recombinant fusion proteins as potent tumoricidal agents.It is perceptible that the strategies for cancer therapy are being innovated by this novel therapeutic approach.

  11. Exchange Rate Regime Choice

    2006-01-01

    The choice of an adequate exchange rate regime proves to be a highly sensitive field within which the economic authorities present and confirm themselves. The advantages and disadvantages of fixed and flexible exchange rate regimes, which have been quite relativized from the conventional point of view, together with simultaneous, but not synchronised effects of structural and external factors, remain permanently questioned throughout a complex process of exchange rate regime decision making. ...

  12. 纳米尿激酶的性状研究%Characterization of urokinase chitosan nanoparticles

    金海将; 张皓; 张柏根; 孙敏莉

    2011-01-01

    背景:尿激酶半衰期短,需持续大量给药,并发症也不小,故有必要研制具有缓释作用的溶栓药物.目的:了解包载尿激酶的水溶性壳聚糖纳米粒子的性状.方法:将壳聚糖和三聚磷酸钠以离子凝集法制备尿激酶纳米粒子后,用透射电镜观察其形态,采用粒径仪测纳米粒子粒径,酶标仪比色法测粒子包封率,纳米粒子冻干法测其载药量,并检测体内外纳米粒子缓释特性.结果与结论:当壳聚糖、三聚磷酸钠、尿激酶的质量比为7∶1∶1时,不仅溶液稳定,形成的纳米粒子粒径小,而且包封率和载药量均合适.制备出包封率最高达94.8%的尿激酶纳米粒子,载药量为14.5%,此时平均粒径236 nm,透射电镜观察示粒子形态较规则,呈球形;粒子具有较好的缓释性能;表明将尿激酶包被于纳米粒子中,避免了消化酶的直接作用,延长了半衰期,不仅在体内能保持较长时间的活性,而且具有明显的缓释效果.%BACKGROUND: Because urokinase has a short half-life, and has a sustained large dose, resulting in in negligible complications,it is necessary to develops low- release thrombolytic drugs .OBJECTIVE: To study the characterization of urokinase chitosan nanoparticles.METHODS: The nanoparticles were prepared via the self-assembly of chitosan and sodium tripolyphosphate. Themorphololywas observed by transmission electron microscopy (TEM). Particle size was measured with particle size instrument Theencapsulation efficiency was tested by ELISA Reader. Drug loading efficiency was measured by the way of weighing lyophiizedpowder. Release characteristics of the nanoparticles were investigated both in vitro and in vivo.RESULT SAND CONCLUSION: When the mass ratio of chitosan, sodium trip olyphosph ate, and ur ok in as was 7:1:1, the solutionwas stable, the nanoparticle size was small, and drug encapsulation efficiency and loading efficiency were appropriate. When thehighest encapsulation

  13. Increased expression of urokinase plasminogen activator and its cognate receptor in human seminomas

    The urokinase plasminogen activating system (uPAS) is implicated in neoplastic progression and high tissue levels of uPAS components correlate with a poor prognosis in different human cancers. Despite that, relative few studies are available on the expression and function of the uPAS components in human seminomas. In the present study we characterized the expression of the urokinase plasminogen activator (uPA), its cognate receptor (uPAR) and the uPA inhibitors PAI-1 and PAI-2 in normal human testis and seminomas. The expression of the above genes was evaluated by means of quantitative RT-PCR, western blot, zymographic analysis and immunohistochemistry. Quantitative RT-PCR analysis of 14 seminomas demonstrated that uPA and uPAR mRNAs were, with respect to control tissues, increased in tumor tissues by 3.80 ± 0.74 (p < 0.01) and 6.25 ± 1.18 (p < 0.01) fold, respectively. On the other hand, PAI-1 mRNA level was unchanged (1.02 ± 0.24 fold), while that of PAI-2 was significantly reduced to 0.34 ± 0.18 (p < 0.01) fold. Western blot experiments performed with protein extracts of three seminomas and normal tissues from the same patients showed that uPA protein levels were low or undetectable in normal tissues and induced in tumor tissues. On the same samples, zymographic analysis demonstrated increased uPA activity in tumor tissue extracts. Western blot experiments showed that also the uPAR protein was increased in tumor tissues by 1.83 ± 0.15 fold (p < 0.01). The increased expression of uPA and uPAR was further confirmed by immunohistochemical staining performed in 10 seminomas and autologous uninvolved peritumoral tissues. Finally, variation in the mRNA level of PAI-1 significantly correlated with tumor size. We demonstrated the increased expression of uPA and uPAR in human seminomas with respect to normal testis tissues, which may be relevant in testicular cancer progression

  14. Sustainable urban regime adjustments

    Quitzau, Maj-Britt; Jensen, Jens Stissing; Elle, Morten;

    2013-01-01

    The endogenous agency that urban governments increasingly portray by making conscious and planned efforts to adjust the regimes they operate within is currently not well captured in transition studies. There is a need to acknowledge the ambiguity of regime enactment at the urban scale. This direc...

  15. Thrombolysis for treating deep venous thrombosis by high-dose urokinase: the usefulness of preventive placement of inferior vena cava filter

    Objective: To investigate the feasibility and efficacy of high-dose urokinase thrombolysis for treating lower limb deep venous thrombosis (DVT) after inferior vena cava (IVC) filter placement. Methods: Thirteen patients of venographically proved DVT underwent preventive IVC filter placement for thrombolysis by high-dose urokinase. Antegrade infusion of high-dose urokinase was performed via the dorsalis pedis vein of the involved lower limb. The total dose of urokinase was 9 000 000 ∼ 16 000 000 units, and the procedure of thrombolysis was performed in ICU ward where the patients were closely monitored clinically and laboratorially. Results: A total of 13 IVC filters were successfully deployed without disposition and migration. The therapeutic effects were divided into four scales as follows: complete disappearance of the venous thrombosis and clinically asymptomatic (n = 2); remarkable recovery characterized by markedly improved clinical symptoms and venographically proved patent lumen in which the diameter was larger than 70% (n = 9); effective treatment indicating improved symptoms to some degrees and venographically proved patent lumen in which the diameter was smaller than 70% ( n = 2); and ineffective treatment (n = 0). No pulmonary embolism and hemorrhage occurred during the procedure of thrombolysis. Conclusion: High-dose urokinase for treating DVT is safe and effective after preventive placement of IVC filter

  16. Endovascular Treatment of Left Iliofemoral Deep Vein Thrombosis Using Urokinase Thrombolysis and Adjunctive Aspiration Thrombectomy

    Suh, Sang Hyun; Lee, Do Yun; Won, Jong Yun [Yonsei University College of Medicine, Seoul (Korea, Republic of)

    2010-02-15

    To evaluate the efficacy of adjunctive aspiration thrombectomy for the treatment of iliofemoral deep vein thrombosis (DVT). 24 patients (9 males and 15 females; mean age, 53 years), treated by aspiration thrombectomy were enrolled in this study. The day after undergoing urokinase (UK) thrombolysis, any residual thrombus over a long segment was treated by aspiration thrombectomy using a 12 Fr long sheath. Residual short-segment (< 10 cm) iliac vein thrombus and/or stenosis were treated with a stent. The evaluation of venous patency was conducted by color Doppler ultrasonography, venography and/or computed tomography. The technical and clinical success rates were 100% and 92%, respectively. Twenty-three patients were treated by UK thrombolysis and iliac stent. The overall patency rate at 1, 2 and 3 years was 85%, 82% and 81%, respectively. Over the course of the follow-up period, occlusion was observed in 4 cases (1 acute and 3 chronic cases). Periprocedural complication occurred in 4 cases (17%) in the form of a minimal hematoma or pain on the puncture site as well as a case of pulmonary embolism at one month after treatment. The adjunctive aspiration thrombectomy with conventional thrombolysis and stent placement can be an effective and safe method in the treatment of left iliofemoral DVT

  17. Endovascular Treatment of Left Iliofemoral Deep Vein Thrombosis Using Urokinase Thrombolysis and Adjunctive Aspiration Thrombectomy

    To evaluate the efficacy of adjunctive aspiration thrombectomy for the treatment of iliofemoral deep vein thrombosis (DVT). 24 patients (9 males and 15 females; mean age, 53 years), treated by aspiration thrombectomy were enrolled in this study. The day after undergoing urokinase (UK) thrombolysis, any residual thrombus over a long segment was treated by aspiration thrombectomy using a 12 Fr long sheath. Residual short-segment (< 10 cm) iliac vein thrombus and/or stenosis were treated with a stent. The evaluation of venous patency was conducted by color Doppler ultrasonography, venography and/or computed tomography. The technical and clinical success rates were 100% and 92%, respectively. Twenty-three patients were treated by UK thrombolysis and iliac stent. The overall patency rate at 1, 2 and 3 years was 85%, 82% and 81%, respectively. Over the course of the follow-up period, occlusion was observed in 4 cases (1 acute and 3 chronic cases). Periprocedural complication occurred in 4 cases (17%) in the form of a minimal hematoma or pain on the puncture site as well as a case of pulmonary embolism at one month after treatment. The adjunctive aspiration thrombectomy with conventional thrombolysis and stent placement can be an effective and safe method in the treatment of left iliofemoral DVT

  18. The modified pulse-spray method using Urokinase in subacute and chronic thrombotic arterial occlusion

    To evaluate the effectiveness and safety of the modified pulse-spray method using Urokinase(UK) in subacute and chronic thrombotic arterial occlusion. Modified pulse-spray methods using UK were performed in seven patients with subacute (1 week-1month) to chronic (1month-5years) occlusive symptoms such as limb pain, claudication and impotence. Angiographic examination revealed thrombotic occlusion of the aorta, common iliac arteries, brachial arterio-venous hemodialysis graft and femoro-popliteal bypass graft. The patients underwent thrombolysis using modified pulse-spray and additional constant infusion of UK. In the presence of underlying stenosis or organized clots, balloon angioplasty or stent placement was performed. Complete lysis was obtained in five of seven patients. For initial lysis, the mean dose of UK was 420,000 units, and the mean modified pulse-spray time was 50 minutes. Mean total dose of UK and mean total time for complete lysis were 800,000 units and 161 minutes, respectively. Thrombolysis of the femoro-popliteal bypass graft failed due to severe occlusion of the distal anastomosis. Partial lysis was achieved in one patient with aorto-illac occlusion, but further thrombolysis was stopped due to bleeding at the puncture site. The modified pulse-spray method using UK is effective in treating subacute and chronic arterial thrombotic occlusion. It augments the speed, safety and efficacy of thrombolysis. When underlying stenosis or organized clots remain after thrombolysis, ballon angioplasty or stent placement would be helpful

  19. [Preparation of novel magnetic dextran affinity adsorbents and their application to purify urokinase].

    Dong, Y S; Liang, F; Yu, X Y; Guo, L A; Chang, J H

    2001-01-01

    The reverse phase suspension and embedment technique were adopted to prepare magnetic dextran microsphere (MDMS). The dispersion medium was mixture of some organic solvents. Span-80 was used as stabilizer. The aqueous dextran with magnetic fluid was suspended in dispersion medium with epichlorohydrin as cross-linking reagent. The mixture was stirred for 30 minutes at room temperature and then heated at 70 degrees C for 4 hours, MDMS was thus obtained. MDMS was activated by epichlorohydrin on which 6-aminohexanoic acid, glycine or ethylene diamine was bonded as spacers. Then it was coupled with p-aminobenzamide, L-arginine methyl ester or guanidohexanoic acid and five magnetic affinity adsorbents were prepared. The MDMS was polydisperse particles with the size of 50-300 meshes and the content of Fe3O4 was about 6.2 per cent in the MDMS. Influence of some parameters such as viscosity and density of organic phase, the volume ratio of organic and aqueous phase, the quantity of surfactant and stirring speed on preparing MDMS was studied. Magnetic affinity adsorbents were used to purify crude urokinase in a bath mode and the effect of coupling reagents and ligands on results of purification was discussed. The bioactivity recovery was 40.0 to 60.7 per cent, the purification-fold was between 14.9 and 32.8, and the adsorptive capacity varies from 89 mg to 121 mg per milliliter of adsorbent. PMID:12541840

  20. Intra-Carotid Urokinase thrombolytic therapy in acute cerebral infarction: a preliminary study

    We conducted a pilot study to evaluate the possibility that the intraarterial thrombolytic therapy might lead to recanalization of the acutely occluded cerebral arteries and subsequent clinical improvement in patients with acute cerebral infarction. Mean time from the onset of symptoms to the start of treatment and mean dosage of thrombolytic agent, Urokinase, were 6.4 hours and 1,260,000 units, respectively. Seven of 12 cases (58%) with acute cerebral infarction demonstrated successful recanalization. Neurological evaluation at one week and three months after the onset of symptoms suggested better outcome in the cases with recanalization. Repeat CT scan at 24 hours and one week after the procedure demonstrated the evidence of hemorrhagic infarction in the infarcted territories in five cases (41%), but clinical deteriorations were observed in only 2 cases. Though statistical analysis could not be done because the limited number of cases, these results suggest that the intraarterial thrombolytic therapy had a role in the management of acute cerebral infarction

  1. Soluble Urokinase Receptors in Focal Segmental Glomerulosclerosis: A Review on the Scientific Point of View

    Saleem, Moin A.; Meijers, Björn

    2016-01-01

    Focal segmental glomerulosclerosis (FSGS) is one of the primary glomerular disorders in both children and adults which can progress to end-stage renal failure. Although there are genetic and secondary causes, circulating factors have also been regarded as an important factor in the pathogenesis of FSGS, because about 40% of the patients with FSGS have recurrence after renal transplantation. Soluble urokinase-type plasminogen activator receptor (suPAR) is a soluble form of uPAR, which is a membrane-bound protein linked to GPI in various immunologically active cells, including podocytes. It has recently been suggested as a potential circulating factor in FSGS by in vitro podocyte experiments, in vivo mice models, and human studies. However, there have also been controversies on this issue, because subsequent studies showed conflicting results. suPAR levels were also increased in patients with other glomerular diseases and were inversely correlated with estimated glomerular filtration rate. Nevertheless, there has been no balanced review on this issue. In this review, we compare the conflicting data on the involvement of suPAR in the pathogenesis of FSGS and shed light on interpretation by taking into account many points and the potential variables and confounders influencing serum suPAR levels.

  2. Identification of a Novel Nucleus Protein Involved in the Regulation of Urokinase in 95D Cells

    Chang TONG; Li TAN; Ping LI; Yun-Song ZHU

    2005-01-01

    The urokinase-type plasminogen activator (uPA) plays an important role in cellular invasion.By using the downstream part of a 74 bp DNA region called the cooperation mediator (COM) of the uPA promoter as a bait sequence in the yeast one-hybrid screen, a gene called PBK1 was previously cloned from the cDNA library of the 95D lung cancer cell strain. In this study, the intracellular distribution of PBK1 was studied by using the transient transfection of pEGFP-C3-PBK1, and PBK1 was found to be localized in the nucleus. Co-transfection of pEGFP-C3-PBK1 and the deletion mutants of the pGL3-uPA promoter indicated that PBK1 can increase the uPA promoter activity by about 25% and this effect is uPA enhancer-dependent.Western blotting and Enzyme-linked immunoadsordent assay further confirmed that PBK1 can upregulate the expression of uPA. Our results suggest that PBK1 is involved in the regulation of uPA expression, which might provide a new clue to further understanding the regulation mechanism of uPA expression.

  3. Aggregation and retention of human urokinase type plasminogen activator in the yeast endoplasmic reticulum

    Smirnov Vladimir N

    2002-10-01

    Full Text Available Abstract Background Secretion of recombinant proteins in yeast can be affected by their improper folding in the endoplasmic reticulum and subsequent elimination of the misfolded molecules via the endoplasmic reticulum associated protein degradation pathway. Recombinant proteins can also be degraded by the vacuolar protease complex. Human urokinase type plasminogen activator (uPA is poorly secreted by yeast but the mechanisms interfering with its secretion are largely unknown. Results We show that in Hansenula polymorpha overexpression worsens uPA secretion and stimulates its intracellular aggregation. The absence of the Golgi modifications in accumulated uPA suggests that aggregation occurs within the endoplasmic reticulum. Deletion analysis has shown that the N-terminal domains were responsible for poor uPA secretion and propensity to aggregate. Mutation abolishing N-glycosylation decreased the efficiency of uPA secretion and increased its aggregation degree. Retention of uPA in the endoplasmic reticulum stimulates its aggregation. Conclusions The data obtained demonstrate that defect of uPA secretion in yeast is related to its retention in the endoplasmic reticulum. Accumulation of uPA within the endoplasmic reticulum disturbs its proper folding and leads to formation of high molecular weight aggregates.

  4. EXPRESSION AND SIGNIFICANCE OF UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN BREAST CANCER

    XIAO Jiping; ZHANG Guangde; XIA Wenhua; CHENG Deji

    1999-01-01

    Objective: To study the expression and clinical significance of urokinase-type plasminogen activator (uPA) in breast cancer. Methods: Applying streptavidin-biotin complex (SABC) immunohistochemical technique, expression of uPA was studied in 100 patients with primary breast cancer. Results: There were 55 patients with high uPA expression, and 45 with lower expression. There was significant correlation between uPA expression and TNM stage, lymph node status, and the tumor size. Neither age, menopausal status, nor ER status was significantly related with level of uPA expression. The patients with high expression of uPA had significantly shorter disease-free survival (DFS)and overall survival (OS) than did those with low expression of uPA. Univariate analysis showed that uPA as a prognostic factor was of similar magnitude to lymph node status and TNM stage, but stronger than that of ER status and tumor size. UPA was an independent prognostic factor affecting disease-free survival and overall survival. Conclusion: uPA appears to be a strong and independent biologic marker for predicting prognosis of breast cancer.

  5. Tyk2 mediates effects of urokinase on human vascular smooth muscle cell growth

    The urokinase (uPA)/uPA receptor (uPAR) system plays a role in the response of the vessel wall to injury, presumably by modulating vascular smooth muscle cell (VSMC) functional behaviour. The Jak/Stat signaling pathway has been implicated to mediate the uPA/uPAR-directed cell migration and proliferation in VSMC. We have therefore investigated the underlying molecular mechanisms, which remained not completely understood. In particular, we aimed at identification of the kinase involved in the signaling cascade leading to Stat1 phosphorylation by uPA and its impact on VSMC growth. We performed expression in VSMC of kinase-deficient mutant forms of the Janus kinases Jak1 and Tyk2 and used different cell culture models imitating the response to vascular injury. We provide evidence that Tyk2, but not Jak1, mediates uPA-induced Stat1 phosphorylation and VSMC growth inhibition and suggest a novel function for Tyk2 as an important modulator of the uPA-directed VSMC functional behaviour at the place of injury

  6. Leukemia inhibitory factor promote trophoblast invasion via urokinase-type plasminogen activator receptor in preeclampsia.

    Zheng, Qin; Dai, Kuixing; Cui, Xinyuan; Yu, Ming; Yang, Xuesong; Yan, Bin; Liu, Shuai; Yan, Qiu

    2016-05-01

    Preeclampsia is a pregnancy-related syndrome which can cause perinatal mortality and morbidity. Inadequate invasion by trophoblast cells may lead to poor perfusion of the placenta, even result in preeclampsia. Understanding the molecular mechanisms underlying placentation facilitates the better intervention of preeclampsia. Urokinase-type plasminogen activator receptor (uPAR) is involved in the physiological and pathological processes. Leukemia inhibitory factor (LIF) is an important regulator in the establishment of pregnancy. However, the expression of uPAR in preeclamptic patients and its relationship with LIF remains unclear. In the current study, we found that the level of uPAR was relatively lower in the placentas from preeclamptic patients as compared with normal pregnant women. LIF promoted trophoblast cell outgrowth by upregulating uPAR in an explants culture, and LIF also enhanced migration and invasion potential through uPAR in trophoblast JAR and JEG-3 cell lines, and with increased gelatinolytic activities of matrix metalloproteinase 2 (MMP-2). The effect of LIF and uPAR on trophoblast migration and invasion was mediated by PI3K/AKT signaling pathway. Our data indicates the roles of LIF in promoting trophoblast migration and invasion through uPAR and suggest that abnormal expression of uPAR might be associated with the etiology of preeclampsia. PMID:27133045

  7. Exchange rate regime choice

    Beker Emilija

    2006-01-01

    Full Text Available The choice of an adequate exchange rate regime proves to be a highly sensitive field within which the economic authorities present and confirm themselves. The advantages and disadvantages of fixed and flexible exchange rate regimes, which have been quite relativized from the conventional point of view, together with simultaneous, but not synchronized effects of structural and external factors, remain permanently questioned throughout a complex process of exchange rate regime decision making. The paper reflects the attempt of critical identification of the key exchange rate performances with emphasis on continuous non-uniformity and (uncertainty of shelf life of a relevant choice.

  8. Structure, function and expression on blood and bone marrow cells of the urokinase-type plasminogen activator receptor, uPAR

    Plesner, T; Behrendt, N; Ploug, M

    1997-01-01

    Several important functions have been assigned to the receptor for urokinase-type plasminogen activator, uPAR. As implied by the name, uPAR was first identified as a high affinity cellular receptor for urokinase plasminogen activator (uPA). It mediates the binding of the zymogen, pro-uPA, to the...... shown that urokinase-type plasminogen activator (uPAR) is expressed as a differentiation antigen on cells of the myelomonocytic lineage and as an activation antigen on monocytes and T lymphocytes. Neutrophils contain intracellular reservoirs of uPAR that are translocated to the plasma membrane upon...... activation, and neutrophils from patients with the rare blood disease paroxysmal nocturnal hemoglobinuria (PNH) that fail to express glycosyl-phosphatidylinositol-anchored proteins including uPAR, show a very significantly reduced transmigration over an endothelial barrier. Cell-associated plasminogen...

  9. Cytokines in cerebrospinal fluid of neurosyphilis patients: Identification of Urokinase plasminogen activator using antibody microarrays.

    Lu, Ping; Zheng, Dao-Cheng; Fang, Chang; Huang, Jin-Mei; Ke, Wu-Jian; Wang, Liu-Yuan; Zeng, Wei-Ying; Zheng, He-Ping; Yang, Bin

    2016-04-15

    Little is known regarding protein responses to syphilis infection in cerebrospinal fluid (CSF) of patients presenting with neurosyphilis. Protein and antibody arrays offer a new opportunity to gain insights into global protein expression profiles in these patients. Here we obtained CSF samples from 46 syphilis patients, 25 of which diagnosed as having central nervous system involvement based on clinical and laboratory findings. The CSF samples were then analyzed using a RayBioH L-Series 507 Antibody Array system designed to simultaneously analyze 507 specific cytokines. The results indicated that 41 molecules showed higher levels in patients with neurosyphilis in comparison with patients without neural involvement. For validation by single target ELISA, we selected five of them (MIP-1a, I-TAC/CXCL11, Urokinase plasminogen activator [uPA], and Oncostatin M) because they have previously been found to be involved in central nervous system (CNS) disorders. The ELISA tests confirmed that uPA levels were significantly higher in the CSF of neurosyphilis patients (109.1±7.88pg/ml) versus patients without CNS involvement (63.86±4.53pg/ml, p<0.0001). There was also a clear correlation between CSF uPA levels and CSF protein levels (p=0.0128) as well as CSF-VDRL titers (p=0.0074) used to diagnose neurosyphilis. No significant difference between the two groups of patients, however, was found in uPA levels in the serum, suggesting specific activation of the inflammatory system in the CNS but not the periphery in neurosyphilis patients. We conclude that measurements of uPA levels in CSF may be an additional parameter for diagnosing neurosyphilis. PMID:27049560

  10. Hypoxia stimulates urokinase receptor expression through a heme protein-dependent pathway.

    Graham, C H; Fitzpatrick, T E; McCrae, K R

    1998-05-01

    Hypoxia underlies a number of biologic processes in which cellular migration and invasion occur. Because earlier studies have shown that the receptor for urokinase-type plasminogen activator (uPAR) may facilitate such events, we studied the effect of hypoxia on the expression of uPAR by first trimester human trophoblasts (HTR-8/SVneo) and human umbilical vein endothelial cells (HUVEC). Compared with control cells cultured under standard conditions (20% O2), HTR-8/SVneo cells and HUVEC cultured in 1% O2 expressed more uPAR, as determined by flow cytometric and [125I]-prourokinase ligand binding analyses. Increased uPAR expression paralleled increases in uPAR mRNA. The involvement of a heme protein in the hypoxia-induced expression of uPAR was suggested by the observations that culture of cells with cobalt chloride, or sodium 4, 5-dihydroxybenzene-1,3-disulfonate (Tiron), an iron-chelating agent, also stimulated uPAR expression, and that the hypoxia-induced uPAR expression was inhibited by adding carbon monoxide to the hypoxic atmosphere. Culture of HTR-8/SVneo cells with vascular endothelial growth factor (VEGF) did not increase uPAR mRNA levels, suggesting that the hypoxia-mediated effect on uPAR expression by these cells did not occur through a VEGF-dependent mechanism. The functional importance of these findings is suggested by the fact that HTR-8/SVneo cells cultured under hypoxia displayed higher levels of cell surface plasminogen activator activity and greater invasion through a reconstituted basement membrane. These results suggest that hypoxia may promote cellular invasion by stimulating the expression of uPAR through a heme protein-dependent pathway. PMID:9558386

  11. World Nonproliferation Regime

    Ouyang Liping; Wu Xingzuo

    2007-01-01

    2006 witnessed an intense struggle between nuclear proliferation and nonproliferation. Iran's nuclear issue and North Korea's nuclear test have cast a deep shadow over the current international nonproliferation regime. The international contest for civil nuclear development became especially fierce as global energy prices went up. Such a situation , to some extent, accelerated the pace of nuclear proliferation. Furthermore, the existing international nonproliferation regime, based upon the Nuclear Nonproliferation Treaty (NPT), was affected by loopholes, and the U.S. failed in its ambition to unite other forces to mend fences. The international community needs to come up with a comprehensive and long-term strategy to meet the demand for an effective future nonproliferation regime in a healthy nuclear order.

  12. Dimagrire in regime mediatico

    Boutaud, Jean-Jacques

    2012-01-01

    publication en italien : BOUTAUD Jean-Jacques, « Dimagrire in regime mediatico », Dietetica e semiotica. Regimi di Senso, a cura di Dario Mangano e Gianfranco Marrone, Mimesis Edizioni, Milano, 2013, p. 19-38 Il titolo può leggersi in molti modi. Per esempio: dietetica e semiotica sono due regimi di senso, uno alimentare e l'altro comunicativo, con pari dignità, dunque da paragonare fra loro. Oppure: grazie alla semiotica la dietetica appare, non più come un regime alimentare, ma come un r...

  13. Discrimination of different forms of the murine urokinase plasminogen activator receptor on the cell surface using monoclonal antibodies

    Rasch, M.G.; Pass, J.; Illemann, M.;

    2008-01-01

    The urokinase plasminogen activator receptor (uPAR) is a versatile three-domain GPI-anchored protein, which binds urokinase plasminogen activator (uPA) and thereby focalises plasminogen activation on the cell surface. Generation of a proteolytic potential is essential in both normal physiological...... murine monocyte macrophage-like P388D.1 cells, we have now generated and characterised two high-affinity murine mAbs, mR3 and mR4, raised against murine uPAR. mR3 was found to recognise an epitope located in domain I of uPAR. Surface plasmon resonance analyses and cell binding studies revealed that this...... mAb was able to bind preformed complexes of murine pro-uPA and murine uPAR. In contrast, mR4 recognises domains II-III in uPAR and does not bind preformed pro-uPA-uPAR complexes in similar analyses. Immunofluorescence microscopy of P388D.1 cells revealed that mR3 stained the cells equally well in...

  14. Soluble urokinase plasminogen activator receptor as a prognostic marker of all-cause and cardiovascular mortality in a black population

    Botha, Shani; Fourie, Carla M T; Schutte, Rudolph; Eugen-Olsen, Jesper; Pretorius, Ronel; Schutte, Aletta E

    2015-01-01

    BACKGROUND: Elevated inflammatory markers such as C-reactive protein (CRP) and interleukin-6 (IL-6) are well-known risk factors for cardiovascular mortality. The less familiar marker, soluble urokinase plasminogen activator receptor (suPAR), is known to predict cancer, infections and all-cause mo......BACKGROUND: Elevated inflammatory markers such as C-reactive protein (CRP) and interleukin-6 (IL-6) are well-known risk factors for cardiovascular mortality. The less familiar marker, soluble urokinase plasminogen activator receptor (suPAR), is known to predict cancer, infections and all.......39, 95% CI 1.17-1.65) predicted all-cause mortality, while only suPAR (HR 1.40, 95% CI 1.04-1.87) and IL-6 (HR 1.61,95% CI 1.10-2.35) predicted cardiovascular mortality. The prognostic value of suPAR was independent of IL-6 and CRP (P≤0.015). CONCLUSION: SuPAR predicted both all-cause and cardiovascular...

  15. The tyrosine phosphatase SHP-2 controls urokinase-dependent signaling and functions in human vascular smooth muscle cells

    The urokinase (uPA)/urokinase receptor (uPAR) multifunctional system is an important mediator of functional behaviour of human vascular smooth muscle cells (VSMC). uPAR associates with platelet-derived growth factor receptor β (PDGFR-β), which serves as a transmembrane adaptor for uPAR in VSMC, to transduce intracellular signaling and initiate functional changes. The precise and rapid propagation of these signaling cascades demands both strict and flexible regulatory mechanisms that remain unexplored. We provide evidence that the tyrosine phosphatase SHP-2 mediates these processes. uPA regulated SHP-2 phosphorylation, catalytic activity, and its co-localization and association with the PDGFR-β. Active PDGFR-β was required for the uPA-induced SHP-2 phosphorylation. uPAR-directed STAT1 pathway was disturbed in cells expressing SHP-2 inactive mutant. Both, cell proliferation and migration were impaired in VSMC with downregulated SHP-2. Elucidating the underlying mechanisms, we found that uPA induced SHP-2 recruitment to lipid rafts. Disruption of rafts abolished uPA-related control of SHP-2 phosphorylation, its association with PDGFR-β and finally the VSMC functional responses. Our results demonstrate that SHP-2 plays an important role in uPA-directed signaling and functional control of human VSMC and suggest that this phosphatase might contribute to the pathogenesis of the uPA-related vascular remodeling

  16. Elevated soluble urokinase receptor values in CSF, age and bacterial meningitis infection are independent and additive risk factors of fatal outcome

    Tzanakaki, G; Paparoupa, M; Kyprianou, M;

    2012-01-01

    The aim of the present study was to evaluate the potential role of cerebrospinal fluid soluble urokinase receptor (suPAR) level, infection and age as risk factors for fatal outcome in patients suspected of having meningitis and/or bacteraemia on admission to hospital. A total of 545 cerebrospinal...

  17. Prognostic value of plasma soluble urokinase plasminogen activator receptor (suPAR) in Danish patients with recurrent epithelial ovarian cancer (REOC)

    Begum, Farah Diba; Høgdall, Estrid V S; Riisbo, Rikke;

    2006-01-01

    The level of the soluble urokinase plasminogen activator receptor (suPAR) is elevated in tumour tissue from several types of cancer. This is the first study aiming to predict the prognosis for survival by the use of a pre-chemotherapeutic plasma suPAR value in 71 patients with recurrent epithelial...

  18. High plasma levels of intact and cleaved soluble urokinase receptor reflect immune activation and are independent predictors of mortality in HIV-1-infected patients

    Ostrowski, Sisse Rye; Piironen, Timo; Høyer-Hansen, Gunilla;

    2005-01-01

    BACKGROUND: High blood levels of soluble urokinase receptor (suPAR) measured by enzyme-linked immunoassay (ELISA) (bulk measurement of 3-domain and 2-domain suPAR [suPAR(I-III), suPAR(II-III)], and suPAR(I-III) ligand complexes) strongly predict mortality in HIV-1-infected patients. This study in...

  19. High plasma levels of intact and cleaved soluble urokinase receptor reflect immune activation and are independent predictors of mortality in HIV-1-infected patients

    Ostrowski, Sisse Rye; Piironen, Timo; Høyer-Hansen, Gunilla;

    2005-01-01

    High blood levels of soluble urokinase receptor (suPAR) measured by enzyme-linked immunoassay (ELISA) (bulk measurement of 3-domain and 2-domain suPAR [suPAR(I-III), suPAR(II-III)], and suPAR(I-III) ligand complexes) strongly predict mortality in HIV-1-infected patients. This study investigated p...

  20. A 55,000-60,000 Mr receptor protein for urokinase-type plasminogen activator. Identification in human tumor cell lines and partial purification

    Nielsen, L S; Kellerman, G M; Behrendt, N;

    1988-01-01

    The iodinated Mr approximately equal to 15,000 amino-terminal fragment (ATF) of the urokinase-type plasminogen activator (u-PA) molecule bound specifically to the cell surface of all of seven cultured human tumor cell lines studied. Cross-linking of iodinated ATF to the cell surface using a bifun...

  1. Urokinase receptor mRNA level and gene transcription are strongly and rapidly increased by phorbol myristate acetate in human monocyte-like U937 cells

    Lund, L R; Rønne, E; Roldan, A L;

    1991-01-01

    We have studied the effect of the tumor promotor phorbol myristate acetate (PMA) on the level of mRNA for the receptor for urokinase-type plasminogen activator (u-PAR) in the human monocyte-like cell line U937. PMA causes an early increase in the u-PAR mRNA level which reaches a maximal 50-fold e...

  2. Presence of urokinase plasminogen activator, its inhibitor and receptor in small cell lung cancer and non-small cell lung cancer

    Pappot, H.; Pfeiffer, P.; Grøndahl Hansen, J.; Skov, B.G.

    1997-01-01

    Spreading of cancer cells is dependent on the combined action of several proteolytic enzymes, such as serine proteases, comprising the urokinase pathway of plasminogen activation. Previous studies of lung cancer indicate that expression, localization and prognostic impact of the components of the...

  3. Soluble urokinase-type plasminogen activator receptor is a novel biomarker predicting acute exacerbation in COPD

    Gumus A

    2015-02-01

    Full Text Available Aziz Gumus,1 Nejat Altintas,2 Halit Cinarka,1 Aynur Kirbas,3 Muge Haziroglu,1 Mevlut Karatas,1 Unal Sahin1 1Department of Pulmonary Medicine, School of Medicine, Recep Tayyip Erdogan University, Rize, Turkey; 2Department of Pulmonary Medicine, School of Medicine, Namik Kemal University, Tekirdag, Turkey; 3Department of Clinical Biochemistry, School of Medicine, Recep Tayyip Erdogan University, Rize, Turkey Background: Chronic obstructive pulmonary disease (COPD is a chronic inflammatory condition, and progresses with acute exacerbations. (AE. During AE, levels of acute phase reactants such as C-reactive protein (CRP and inflammatory cells in the circulation increase. Soluble urokinase-type plasminogen activator receptor (suPAR levels increase in acute viral and bacterial infections and in diseases involving chronic inflammation. The purpose of this study was to investigate the effectiveness of suPAR in predicting diagnosis of AE of COPD (AE-COPD and response to treatment. Methods: The study population consisted of 43 patients diagnosed with AE-COPD and 30 healthy controls. suPAR, CRP, and fibrinogen levels were measured on the first day of hospitalization and on the seventh day of treatment. Results: We found that fibrinogen (P<0.001, CRP (P<0.001, and suPAR (P<0.001 were significantly higher in patients with AE-COPD than in healthy controls. Fibrinogen (P<0.001, CRP (P=0.001, and suPAR (P<0.001 were significantly decreased by the seventh day of treatment. However, the area under receiver operator characteristic curve showed that suPAR is superior to CRP and fibrinogen in distinguishing AE-COPD. There was a correlation between fibrinogen, CRP, and suPAR. However, only fibrinogen was a powerful predictor of suPAR in multiple linear regression. In multiple logistic regression, only suPAR and fibrinogen were strong predictors of AE-COPD (P=0.002 and P=0.014, respectively. Serum suPAR was negatively correlated with forced expiratory volume in 1

  4. Radiation-Induced Hypomethylation Triggers Urokinase Plasminogen Activator Transcription in Meningioma Cells

    Kiran Kumar Velpula

    2013-02-01

    Full Text Available Our previous studies have shown the role of radiation-induced urokinase plasminogen activator (uPA expression in the progression of meningioma. In the present study, we investigated whether modulation of DNA methylation profiles could regulate uPA expression. Initially, radiation treatment was found to induce hypomethylation in meningioma cells with a decrease in DNA (cytosine-5-methyltransferase 1 (DNMT1 and methyl-CpG binding domain protein (MBD expression. However, oxidative damage by H2O2 or pretreatment of irradiated cells with N-acetyl cysteine (NAC did not show any influence on these proteins, thereby indicating a radiation-specific change in the methylation patterns among meningioma cells. Further, we identified that hypomethylation is coupled to an increase in uPA expression in these cells. Azacytidine treatment induced a dose-dependent surge of uPA expression, whereas pre-treatment with sodium butyrate inhibited radiation-induced uPA expression, which complemented our prior results. Methylation-specific polymerase chain reaction on bisulfite-treated genomic DNA revealed a diminished methylation of uPA promoter in irradiated cells. Transfection with small hairpin RNA (shRNA-expressing plasmids targeting CpG islands of the uPA promoter showed a marked decline in uPA expression with subsequent decrease in invasion and proliferation of meningioma cells. Further, radiation treatment was found to recruit SP1 transcription factor, which was abrogated by shRNA treatment. Analysis on signaling events demonstrated the activation of MAP kinase kinase (MEK-extracellular signal-regulated kinase (ERK in radiation-treated cells, while U0126 (MEK/ERK inhibitor blocked hypomethylation, recruitment of SP1, and uPA expression. In agreement with our in vitro data, low DNMT1 levels and high uPA were found in intracranial tumors treated with radiation compared to untreated tumors. In conclusion, our data suggest that radiation-mediated hypomethylation

  5. The “Lipid Accumulation Product” Is Associated with 2-Hour Postload Glucose Outcomes in Overweight/Obese Subjects with Nondiabetic Fasting Glucose

    Malavazos, Alexis Elias; Cereda, Emanuele; Ermetici, Federica; Caccialanza, Riccardo; Briganti, Silvia; Rondanelli, Mariangela; Morricone, Lelio

    2015-01-01

    “Lipid accumulation product” (LAP) is a continuous variable based on waist circumference and triglyceride concentration previously associated with insulin resistance. We investigated the accuracy of LAP in identifying oral glucose tolerance test (OGTT) abnormalities and compared it to the homeostasis model assessment of insulin resistance (HOMA-IR) in a population of overweight/obese outpatients presenting with nondiabetic fasting glucose. We studied 381 (male: 23%) adult (age: 18–70 years) overweight/obese Caucasians (body mass index: 36.9 ± 5.4 Kg/m2) having fasting plasma glucose < 7.0 mmol/L. OGTT was used to diagnose unknown glucose tolerance abnormalities: impaired glucose tolerance (IGT) and type-2 diabetes mellitus (T2-DM). According to OGTT 92, subjects had an IGT and 33 were diagnosed T2-DM. Logistic regression analysis detected a significant association for both LAP and HOMA-IR with single (IGT and T2-DM) and composite (IGT + T2-DM) abnormal glucose tolerance conditions. However, while the association with diabetes was similar between LAP and HOMA-IR, the relationship with IGT and composite outcomes by models including LAP was significantly superior to those including HOMA-IR (P = 0.006 and P = 0.007, resp.). LAP seems to be an accurate index, performing better than HOMA-IR, for identifying 2-hour postload OGTT outcomes in overweight/obese patients with nondiabetic fasting glucose. PMID:25792981

  6. The “Lipid Accumulation Product” Is Associated with 2-Hour Postload Glucose Outcomes in Overweight/Obese Subjects with Nondiabetic Fasting Glucose

    Alexis Elias Malavazos

    2015-01-01

    Full Text Available “Lipid accumulation product” (LAP is a continuous variable based on waist circumference and triglyceride concentration previously associated with insulin resistance. We investigated the accuracy of LAP in identifying oral glucose tolerance test (OGTT abnormalities and compared it to the homeostasis model assessment of insulin resistance (HOMA-IR in a population of overweight/obese outpatients presenting with nondiabetic fasting glucose. We studied 381 (male: 23% adult (age: 18–70 years overweight/obese Caucasians (body mass index: 36.9 ± 5.4 Kg/m2 having fasting plasma glucose < 7.0 mmol/L. OGTT was used to diagnose unknown glucose tolerance abnormalities: impaired glucose tolerance (IGT and type-2 diabetes mellitus (T2-DM. According to OGTT 92, subjects had an IGT and 33 were diagnosed T2-DM. Logistic regression analysis detected a significant association for both LAP and HOMA-IR with single (IGT and T2-DM and composite (IGT + T2-DM abnormal glucose tolerance conditions. However, while the association with diabetes was similar between LAP and HOMA-IR, the relationship with IGT and composite outcomes by models including LAP was significantly superior to those including HOMA-IR (P=0.006 and P=0.007, resp.. LAP seems to be an accurate index, performing better than HOMA-IR, for identifying 2-hour postload OGTT outcomes in overweight/obese patients with nondiabetic fasting glucose.

  7. Complex regimes of synchronization

    Yeldesbay, Azamat

    2014-01-01

    Synchronization is a fundamental phenomenon in nature. It can be considered as a general property of self-sustained oscillators to adjust their rhythm in the presence of an interaction. In this work we investigate complex regimes of synchronization phenomena by means of theoretical analysis, numerical modeling, as well as practical analysis of experimental data. As a subject of our investigation we consider chimera state, where due to spontaneous symmetry-breaking of an initially ho...

  8. Combined use of serum adiponectin and tumor necrosis factor-alpha receptor 2 levels was comparable to 2-hour post-load glucose in diabetes prediction.

    Yu-Cho Woo

    Full Text Available BACKGROUND: Adipose tissue inflammation and dysregulated adipokine secretion are implicated in obesity-related insulin resistance and type 2 diabetes. We evaluated the use of serum adiponectin, an anti-inflammatory adipokine, and several proinflammatory adipokines, as biomarkers of diabetes risk and whether they add to traditional risk factors in diabetes prediction. METHODS: We studied 1300 non-diabetic subjects from the prospective Hong Kong Cardiovascular Risk Factor Prevalence Study (CRISPS. Serum adiponectin, tumor necrosis factor-alpha receptor 2 (TNF-α R2, interleukin-6 (IL-6, adipocyte-fatty acid binding protein (A-FABP and high-sensitivity C-reactive protein (hsCRP were measured in baseline samples. RESULTS: Seventy-six participants developed diabetes over 5.3 years (median. All five biomarkers significantly improved the log-likelihood of diabetes in a clinical diabetes prediction (CDP model including age, sex, family history of diabetes, smoking, physical activity, hypertension, waist circumference, fasting glucose and dyslipidaemia. In ROC curve analysis, "adiponectin + TNF-α R2" improved the area under ROC curve (AUC of the CDP model from 0.802 to 0.830 (P = 0.03, rendering its performance comparable to the "CDP + 2-hour post-OGTT glucose" model (AUC = 0.852, P = 0.30. A biomarker risk score, derived from the number of biomarkers predictive of diabetes (low adiponectin, high TNF-α R2, had similar performance when added to the CDP model (AUC = 0.829 [95% CI: 0.808-0.849]. CONCLUSIONS: The combined use of serum adiponectin and TNF-α R2 as biomarkers provided added value over traditional risk factors for diabetes prediction in Chinese and could be considered as an alternative to the OGTT.

  9. Urokinase plasminogen activator and plasminogen activator inhibitor type-1 in nonsmall-cell lung cancer: relation to prognosis and angiogenesis

    Offersen, Birgitte Vrou; Pfeiffer, Per; Andreasen, Peter;

    2007-01-01

    BACKGROUND: Urokinase plasminogen activator (uPA) and plasminogen activator inhibitor type-1 (PAI-1) have previously been suggested as prognostic markers in nonsmall-cell lung carcinomas (NSCLC). We investigate whether uPA and PAI-1 are prognostic markers in NSCLC and whether they are related to...... sandwich ELISA method. RESULTS: Both uPA and PAI-1 were independent of classical histopathological parameters as well as of microvessel density and vascular pattern. Using death within the first 5 years as endpoint, neither of the factors were prognostic markers in univariate analysis, however......, significantly higher levels of uPA and PAI-1 were seen in tumours with an angiogenic vascular pattern. In multivariate analysis, high disease stage (P<0.0001), adenocarcinoma (P=0.007), old age (P=0.02), and presence of an angiogenic pattern (P=0.05) were identified as independent markers of death within 5...

  10. Soluble urokinase receptor levels in plasma during 5 years of highly active antiretroviral therapy in HIV-1-infected patients

    Ostrowski, Sisse R; Katzenstein, Terese L; Piironen, Timo;

    2004-01-01

    High blood levels of the soluble urokinase receptor (suPAR) strongly predict increased mortality in human immunodeficiency virus-1 (HIV-1)-infected patients. This study investigated the plasma concentration of suPAR in 29 treatment-naive HIV-1-infected patients during 5 years treatment with highly...... active antiretroviral therapy (HAART). Plasma suPAR decreased after introducing HAART, most pronounced during the first treatment year. The change in plasma suPAR was independent of changes in viral replication and CD4+ cells but it was strongly correlated with plasma levels of the soluble TNF receptor...... II. Compared with healthy individuals, plasma suPAR and sTN-FrII was increased in untreated patients. After initiating HAART, plasma sTNFrII remained increased whereas plasma suPAR decreased to a level comparable with healthy individuals. The present data indicate that the circulating suPAR level is...

  11. The urokinase receptor (uPAR) and the uPAR-associated protein (uPARAP/Endo180)

    Behrendt, Niels

    2004-01-01

    processes involve a highly organized interplay between proteases and their cellular binding sites as well as specific substrates and internalization receptors. This review article is focused on two components, the urokinase plasminogen activator receptor (uPAR) and the uPAR-associated protein (uPARAP, also......The breakdown of the barriers formed by extracellular matrix proteins is a pre-requisite for all processes of tissue remodeling. Matrix degradation reactions take part in specific physiological events in the healthy organism but also represent a crucial step in cancer invasion. These degradation...... designated Endo180), that are considered crucially engaged in matrix degradation. uPAR and uPARAP have highly diverse functions, but on certain cell types they interact with each other in a process that is still incompletely understood. uPAR is a glycosyl-phosphatidylinositol-anchored glycoprotein on the...

  12. The complex between urokinase (uPA) and its type-1 inhibitor (PAI-1) in pulmonary adenocarcinoma

    Pappot, Helle; Pedersen, Anders N.; Brünner, Nils;

    2006-01-01

    In a lung cancer population comprising tumor tissue from 99 pulmonary adenocarcinoma patients, the relationship between tumor tissue level of the complex formed of urokinase (uPA) and its type-1 inhibitor (PAI-1) and survival was studied. The study included patient material previously investigated...... patients with low PAI-1 and high uPA-PAI-1 complex (HR = 3.06, p = 0.01). This is the first investigation of the prognostic impact of uPA-PAI-1 complex in a tumor type other than breast cancer, showing low levels of uPA-PAI-1 complex in combination with high levels of PAI-1 to be associated with poor...

  13. Soluble urokinase plasminogen activator receptor levels are elevated and associated with complications in patients with type 1 diabetes

    Theilade, S; Lyngbaek, S; Hansen, T W;

    2015-01-01

    OBJECTIVES: Soluble urokinase plasminogen activator receptor (suPAR) is a marker of inflammation and endothelial dysfunction. We investigated the associations between suPAR and diabetes, including diabetes duration and complications, in patients with type 1 diabetes. DESIGN, SETTING AND SUBJECTS...... diabetes and is associated with diabetes duration and complications independent of other risk factors. suPAR is a potential novel risk marker for the management of diabetes.......: From 2009 to 2011, 667 patients with type 1 diabetes and 51 nondiabetic control subjects were included in a cross-sectional study at Steno Diabetes Center, Gentofte, Denmark. suPAR levels were measured with an enzyme-linked immunosorbent assay. MAIN OUTCOME MEASURES: The investigated diabetic...

  14. Risk Factors Associated with Serum Levels of the Inflammatory Biomarker Soluble Urokinase Plasminogen Activator Receptor in a General Population

    Haupt, Thomas H; Kallemose, Thomas; Ladelund, Steen;

    2014-01-01

    .001). An unhealthy diet and alcohol abstinence in men were also associated with higher suPAR levels. Physical activity in leisure time had a modest impact on suPAR levels in univariate analysis, but not in the final adjusted model. In conclusion, smoking and morbid obesity were strongly associated with higher serum...... suPAR levels in this general population. Diet and alcohol consumption also seemed to impact suPAR levels. Lifestyle changes are likely to affect suPAR since ex-smokers had suPAR levels comparable to those of never-smokers.......The soluble urokinase plasminogen activator receptor (suPAR) is a biomarker of mortality risk in various patient populations. However, little is known about the implications of lifestyle for suPAR levels in the general population. Lifestyle, demographic, and cardiovascular disease (CVD) risk factor...

  15. Preclinical evaluation of a urokinase plasminogen activator receptor-targeted nanoprobe in rhesus monkeys

    Chen Y

    2015-10-01

    Full Text Available Yushu Chen,1 Li Gong,2 Ning Gao,3 Jichun Liao,1 Jiayu Sun,1 Yuqing Wang,1 Lei Wang,1 Pengjin Zhu,1 Qing Fan,1 Yongqiang Andrew Wang,4 Wen Zeng,2 Hui Mao,3 Lily Yang,5 Fabao Gao11Molecular Imaging Center, Department of Radiology, West China Hospital, Sichuan University, Chengdu, 2Sichuan Primed Bio-Tech Group Co, Ltd, Chengdu, People’s Republic of China; 3Department of Radiology and Imaging Sciences, Emory University School of Medicine, Atlanta, GA, 4Ocean NanoTech, LLC, San Diego, CA, 5Department of Surgery, Emory University School of Medicine, Atlanta, GA, USAPurpose: To translate a recombinant peptide containing the amino-terminal fragment (ATF of urokinase plasminogen activator receptor-targeted magnetic iron oxide (IO nanoparticles (uPAR-targeted human ATF-IONPs into clinical applications, we conducted a pilot study to evaluate the toxicity and pharmacokinetics of this nanoparticle in normal rhesus monkeys.Methods: We assessed the changes in the following: magnetic resonance imaging (MRI signals from pretreatment stage to 14 days posttreatment, serum iron concentrations from 5 minutes posttreatment to 12 weeks posttreatment, routine blood examination and serum chemistry analysis results from pretreatment stage to 12 weeks after administration, and results of staining of the liver with Perls’ Prussian Blue and hematoxylin–eosin at 24 hours and 3 months posttreatment in two rhesus monkeys following an intravenous administration of the targeted nanoparticles either with a polyethylene glycol (ATF-PEG-IONP or without a PEG (ATF-IONP coating.Results: The levels of alkaline phosphatase, alanine transaminase, and direct bilirubin in the two monkeys increased immediately after the administration of the IONPs but returned to normal within 20 days and stayed within the normal reference range 3 months after the injection. The creatinine levels of the two monkeys stayed within the normal range during the study. In addition, red blood cells

  16. Influential factors of clinical outcome of local intra-arterial thrombolysis using urokinase in patients with hyperacute ischemic stroke

    To evaluate the clinical outcome and other relevant factors in cases where local intra-arterial thrombolysis (LIT) is used for the treatment of hyperacute ischemic stroke. Forty-eight hyperacute ischemic stroke patients were treated by LIT, using urokinase, within six hours of ictus, and for evaluation of their neurological status, the national institutes of health stroke scale (NIHSS) score was used. Angiography recanalization was classified according to Mori recanalization grades. Three months after LIT, the outcome was assessed by clinical examination using the modified rankin scale (good outcome: RS=0-3; poor outcome: RS=4-6). In all patients, the findings of pre- and post- LIT CT, and angiography, as well as neurological status and hemorrhagic complications, were also analysed. Thirty-three patients had occlusions of the middle cerebral artery (MCA), and 15, of the internal carotid artery (ICA). The NIHSS score averaged 16.9 at the onset of therapy and 13.5 at 24 hours later. Successful recanalization (Mori grade 3,4) was achieved in 28 (58.3%) of 48 patients, but in 20 (41.7%) the attempt failed. Twenty-two (45.8%) of the 48 patients had a good outcome, but in (54.2%) the outcome was poor. Thirteen (40.6%) of 32 patients with MCA occlusions and 13 (81.2%) of 16 with ICA occlusions had a poor outcome. Eight patients (16.7%) died. Overall, hemorrhages occured in 20 (41.7%) of 48 patients, with symptomatic hemorrhage in ten. Five (50%) of these ten died. LIT using urokinase for hyperacute ischemic stroke is feasible; patients with MCA occlusions had better outcomes than those with ICA occlusions. Hemorrhagic complications of LIT were frequent, and in cases of symptomatic hemorrhage a fatal outcome may be expected

  17. Influential factors of clinical outcome of local intra-arterial thrombolysis using urokinase in patients with hyperacute ischemic stroke

    Song, Jae Min; Yoon, Woong; Kim, Jae Kyu; Seo, Jeong Jin; Heo, Sook Hee; Park, Jin Gyoon; Jeong, Yoon Yeon; Kang, Heoung Keun [Chonam University Hospital, Kwangju (Korea, Republic of)

    2002-10-01

    To evaluate the clinical outcome and other relevant factors in cases where local intra-arterial thrombolysis (LIT) is used for the treatment of hyperacute ischemic stroke. Forty-eight hyperacute ischemic stroke patients were treated by LIT, using urokinase, within six hours of ictus, and for evaluation of their neurological status, the national institutes of health stroke scale (NIHSS) score was used. Angiography recanalization was classified according to Mori recanalization grades. Three months after LIT, the outcome was assessed by clinical examination using the modified rankin scale (good outcome: RS=0-3; poor outcome: RS=4-6). In all patients, the findings of pre- and post- LIT CT, and angiography, as well as neurological status and hemorrhagic complications, were also analysed. Thirty-three patients had occlusions of the middle cerebral artery (MCA), and 15, of the internal carotid artery (ICA). The NIHSS score averaged 16.9 at the onset of therapy and 13.5 at 24 hours later. Successful recanalization (Mori grade 3,4) was achieved in 28 (58.3%) of 48 patients, but in 20 (41.7%) the attempt failed. Twenty-two (45.8%) of the 48 patients had a good outcome, but in (54.2%) the outcome was poor. Thirteen (40.6%) of 32 patients with MCA occlusions and 13 (81.2%) of 16 with ICA occlusions had a poor outcome. Eight patients (16.7%) died. Overall, hemorrhages occured in 20 (41.7%) of 48 patients, with symptomatic hemorrhage in ten. Five (50%) of these ten died. LIT using urokinase for hyperacute ischemic stroke is feasible; patients with MCA occlusions had better outcomes than those with ICA occlusions. Hemorrhagic complications of LIT were frequent, and in cases of symptomatic hemorrhage a fatal outcome may be expected.

  18. Construction, Expression, and Characterization of a Recombinant Annexin B1-Low Molecular Weight Urokinase Chimera in Escherichia coli

    Hong-Li YAN; Wei-Ting WANG; Yan HE; Zhuan-You ZHAO; Yuan-Jian GAO; Yi ZHANG; Shu-Han SUN

    2004-01-01

    To produce a thrombi-targeting plasminogen activator,low molecular weight single-chain urokinase gene(scuPA32k)was spliced with the full-length cDNA of annexin B 1 gene(anxB1)by overlap extension method.The fused gene anxBlscuPA was ligated into pET28a vector,transformed into E.coli BL21-RIL,and then induced to express under the control of T7 promoter.The AnxB 1ScuPA protein expressed amounted to 22% of the total bacterial proteins.The product was refolded,and then purified by using DEAE Sepharose fast flow ion-exchange column and Superdex S-200 gel-filtration column.HPLC analysis revealed that the final purity is about 95%.The specific activity ofAnxB 1ScuPA,measured as amidolytic activity,reached 100,000 IU/mg.It had a similar S2444 catalytic efficiency(kcat/Km)to ScuPA32k,and also showed high activated-platelet membrane-binding activity and anticoagulant activity,indicating that the chimera fully retained the components of enzymatic and membrane-binding activities of the parent molecules.In vivo test revealed that,the dogs administered with AnxB 1ScuPA had less reperfusion time,higher reperfusion ratio,and less bleeding effects than those with urokinase.These findings indicated that AnxB 1ScuPAmight have advantages over current available thrombolytic agents.

  19. Reduced release of intact and cleaved urokinase receptor in stimulated whole-blood cultures from human immunodeficiency virus-1-infected patients

    Ostrowski, S R; Piironen, T; Høyer-Hansen, G;

    2005-01-01

    The blood levels of the soluble forms of the urokinase receptor (suPAR) are increased in human immunodeficiency virus (HIV)-1-infected patients. This study investigated whether the release of urokinase-type plasminogen activator receptor (uPAR) in whole-blood cultures was affected by HIV infection...... controls, whereas the correlation was weaker to leucocytes and nonexisting to circulating suPAR levels in HIV patients. These findings demonstrate that HIV infection affects stimulated and spontaneous uPAR release in whole-blood cultures. Given that high blood levels of suPAR in HIV patients are linked to....... The release of different uPAR forms in whole-blood cultures incubated 24 h with or without phytohemagglutinin and lipopolysaccharide was analysed in 47 HIV patients and 19 controls. suPAR was measured by enzyme-linked immunosorbent assay (ELISA) (bulk-suPAR) and three different time...

  20. Interleukin-1ß-induced expression of the urokinase-type plasminogen activator receptor and its co-localization with MMPs in human articular chondrocytes

    Schwab, W.; Schulze-Tanzil, G.; Mobasheri, A; Dressler, J.; Kotzsch, M.; Shakibaei, M.

    2004-01-01

    The urokinase-type plasminogen activator receptor (uPAR) plays a critical role in cartilage degradation during osteoarthritis as it regulates pericellular proteolysis mediated by serine proteinases. Another important family of proteinases responsible for ECM destruction in arthritis are the matrix metalloproteinases (MMPs). MMPs are regulated by IL- 1ß, a cytokine that plays a pivotal role in pathogenesis of osteoarthritis. This study was undertaken to address ...

  1. Soluble urokinase-type plasminogen activator receptor levels in patients with burn injuries and inhalation trauma requiring mechanical ventilation: an observational cohort study

    Backes, Yara; van der Sluijs, Koenraad F; Tuip de Boer, Anita M; Hofstra, Jorrit Jan; Vlaar, Alexander PJ; Determann, Rogier M; Knape, Paul; David P Mackie; Schultz, Marcus J.

    2011-01-01

    Introduction Soluble urokinase-type plasminogen activator receptor (suPAR) has been proposed as a biologic marker of fibrinolysis and inflammation. The aim of this study was to investigate the diagnostic and prognostic value of systemic and pulmonary levels of suPAR in burn patients with inhalation trauma who need mechanical ventilation. Methods suPAR was measured in plasma and nondirected lung-lavage fluid of mechanically ventilated burn patients with inhalation trauma. The samples were obta...

  2. Phorbol ester induces the biosynthesis of glycosylated and nonglycosylated plasminogen activator inhibitor 2 in high excess over urokinase-type plasminogen activator in human U-937 lymphoma cells

    1987-01-01

    The tumor-promoting phorbol ester PMA induces changes in the histiocytic human lymphoma cell line U-937 akin to cellular differentiation (Ralph, P., N. Williams, M. A. S. Moore, and P. B. Litcofsky, 1982, Cell. Immunol., 71:215-223) and concomitantly stimulates the biosynthesis of plasminogen activator inhibitor 2 (PAI 2) and of urokinase-type plasminogen activator (u-PA). PAI 2 is found in a nonglycosylated intracellular and a glycosylated secreted form. The former appears to be identical to...

  3. Urokinase plasminogen activator (uPA and plasminogen activator inhibitor type-1 (PAI-1 in breast cancer - correlation with traditional prognostic factors

    Lampelj Maja

    2015-12-01

    Full Text Available Background. Urokinase plasminogen activator (uPA and plasminogen activator inhibitor type-1 (PAI-1 play a key role in tumour invasion and metastasis. High levels of both proteolytic enzymes are associated with poor prognosis in breast cancer patients. The purpose of this study was to evaluate the correlation between traditional prognostic factors and uPA and PAI-1 expression in primary tumour of breast cancer patients.

  4. The human urokinase-plasminogen activator gene (PLAU) is located on chromosome 10q24 centromeric to the HOX11 gene

    Stein, P.M.; Stass, S.A.; Kagan, J. (Univ. of Texas, Houston (United States))

    1993-04-01

    Urokinase-plasminogen activator is one of two soluble serine proteases that are produced by humans and that convert plasminogen, an inactive proenzyme present in plasma and other extracellular fluids, to plasmin, a protease with broad substrate specificities. Its activity is involved in processes requiring localized extracellular proteolysis such as fibrinolysis, tissue remodeling, and cell migration. Increased production of urokinase has been associated with cancer metastases. The gene for urokinase-plasminogen activator, PLAU, was mapped to chromosome 10q24-qter. By employing somatic cell genetics, polymerase chain reaction (PCR), and Southern blot analysis, the authors assign PLAU to chromosome 10q24. Human chromosome segment 10q23-q25 contains the genes for terminal deoxynucleotidyltransferase, cytochrome P450IIC, glutamic-oxaloacetic transaminase, and plasma retinol binding protein, which form a syntenic group on murine chromosome 19. It is therfore of interest that PLAU and glutamate dehydrogenase, which are on murine chromosome 14, also map in or close to this region of human chromosome 10.

  5. In vivo effects of urease-producing bacteria involved with the pathogenesis of infection-induced urolithiasis on renal urokinase and sialidase activity.

    du Toit, P J; van Aswegen, C H; Nel, J A; Steyn, P L; Ligthelm, A J; du Plessis, D J

    1995-01-01

    Many hypotheses have been proposed for renal stone formation. It has been argued that with infection-induced renal stones the hydrolysis of urea by bacterial urease increases urinary pH, with consequent stone formation. Unfortunately, this theory is not applicable to the micro-organisms that do not produce urease (e.g. Escherichia coli). It has been recently reported that E. coli reduces the urinary urokinase activity of male rats, but does not influence the urinary sialidase activity. This study has now been expanded to the urease-producing bacteria Proteus mirabilis, Staphylococcus aureus, S. epidermidis, Pseudomonas aeruginosa and Micrococcus luteus. Subcutaneous injections with these bacteria were found to significantly (P urease-producing mammalian skin bacterium, M. luteus, was, however, the exception (P = 0.1079). In contrast to S. epidermidis, P. aeruginosa and M. luteus (P mirabilis and S. aureus had no effect on renal sialidase activity (P Proteus species are predominant in infection-induced renal stones. According to the urokinase-sialidase hypothesis, a decrease in urinary urokinase activity should increase the uromucoid levels, whilst no effect on the urinary sialidase activity should favour conversion of urinary uromucoid to mineralizable matrix. These conditions may lead to renal stone formation. An increase in urinary pH resulting from urease-producing micro-organisms will increase salt precipitation on the uromucoid. It is thus concluded that urease-producing bacteria may play a double role in renal stone formation. PMID:8839391

  6. Political Regimes and Economic Growth

    Carlos Pinho

    2009-12-01

    Full Text Available Are political regimes drivers of economic growth? While political institutions are influenced by economic development, they are in turn a key determinant of the development process. This study builds in the Neoclassical Growth theory to identify the influence of political regimes on economic development through a panel data sample of 170 countries from 1960 to 2000. Results suggest that once fixed effects are considered, the positive relationship between income per capita and political regimes measured by different democracy variables disappears.

  7. Structure-based Engineering of Species Selectivity in the Interaction Between Urokinase and its Receptor: Implication for Preclinical Cancer Therapy

    The high affinity interaction between the urokinase-type plasminogen activator (uPA) and its glycolipid-anchored receptor (uPAR) is decisive for cell surface-associated plasminogen activation. Because plasmin activity controls fibrinolysis in a variety of pathological conditions, including cancer and wound healing, several intervention studies have focused on targeting the uPA · uPAR interaction in vivo. Evaluations of such studies in xenotransplanted tumor models are, however, complicated by the pronounced species selectivity in this interaction. We now report the molecular basis underlying this difference by solving the crystal structure for the murine uPA · uPAR complex and demonstrate by extensive surface plasmon resonance studies that the kinetic rate constants for this interaction can be swapped completely between these orthologs by exchanging only two residues. This study not only discloses the structural basis required for a successful rational design of the species selectivity in the uPA · uPAR interaction, which is highly relevant for functional studies in mouse models, but it also suggests the possible development of general inhibitors that will target the uPA · uPAR interaction across species barriers.

  8. Interventional recanalization of artificial arteriovenous fistula and graft for hemodialysis: angioplasty and pulsed-spray thrombolysis with Urokinase

    To evaluate the effectiveness of percutaneous transluminal angioplasty (PTA) and pul-sed-spray pharmaco-mechanical thrombolysis (PSPMT) using urokinase for the management of insufficient hemodialysis access. Between September 1996 and May 1998, 21 insufficient hemodialysis accesses were treated in 16 patients(3 artificial arteriovenous fistulae, AVF; and 13 arteriovenous graft, AVG). PTA and PSPMT were performed in 6 and 15 cases, respectively, and success and long-term patency rates were evaluated. The overall success rate of PTA and PSPMT for insufficient hemodialysis access was 76. 2%(16/21). The success rates of PTA and PSPMT were 83.3%(5/6) and 73.3%(11/15), respectively. The primary patency rates of PSPMT were 69±12.8% at 6 months and 38±18.6% at 12 months. One of the two initially successful PTAs had been patent for 7 months, and the second PTA was performed at that time due to venous stenosis. The other was patent for 15 months throughout the follow-up period. PTA and PSPMT are effective primary methods for the treatment of insufficient hemodialysis access;success and patency rates were high, and the procedures can be performed repeatedly.=20

  9. Cigarette Smoke and the Induction of Urokinase Plasminogen Activator Receptor In Vivo: Selective Contribution of Isoforms to Bronchial Epithelial Phenotype.

    Portelli, Michael A; Stewart, Ceri E; Hall, Ian P; Brightling, Christopher E; Sayers, Ian

    2015-08-01

    The urokinase plasminogen activator receptor (uPAR) gene (PLAUR) has been identified as an asthma susceptibility gene, with polymorphisms within that gene being associated with baseline lung function, lung function decline, and lung function in a smoking population. Soluble cleaved uPAR (scuPAR), a molecule identified as a marker of increased morbidity and mortality in a number of diseases, has been shown to be elevated in the airways of patients with asthma and in patients with chronic obstructive pulmonary disease. However, the functionality of soluble receptor isoforms and their relationship with an important initiator for obstructive lung disease, cigarette smoke, remains undefined. In this study, we set out to determine the effect of cigarette smoke on soluble uPAR isoforms, its regulatory pathway and the resultant effect on bronchial epithelial cell function. We identified a positive association between cigarette pack-years and uPAR expression in the airway bronchial epithelium of biopsies from patients with asthma (n = 27; P = 0.0485). In vitro, cigarette smoke promoted cleavage of uPAR from the surface of bronchial epithelial cells (1.5× induction; P bronchial epithelial cells. This suggests that cigarette smoke elevates soluble receptor isoforms in bronchial epithelial cells through direct (cleavage) and indirect (messenger RNA expression) means. These findings provide further insight into how cigarette smoke may influence changes in the airways of importance to airway remodeling and obstructive lung disease progression. PMID:25490122

  10. Prognostic significance of circulating intact and cleaved forms of urokinase plasminogen activator receptor in inoperable chemotherapy treated cholangiocarcinoma patients

    Grunnet, M; Christensen, I J; Lassen, U;

    2014-01-01

    determine if pre-treatment serum levels of uPAR forms and a decrease in levels during chemotherapy are predictive of survival in patients with inoperable cholangiocarcinoma. DESIGN AND METHODS: Patients with inoperable cholangiocarcinoma were consecutively included in the training set (n=108). A test set......PAR(I-III)+uPAR(II-III) after 2cycles of chemotherapy was associated with poor survival (HR=1.79, 95% CI:1.08-2.97, p=0.023, n=57). This predictor, however, was not significant in the test set (p=0.21, 26 events in 27 patients). CONCLUSION: The baseline level of uPAR(I-III)+uPAR(II-III) is a predictor of survival in inoperable......BACKGROUND: High levels of intact and cleaved forms of the urokinase-type plasminogen activator receptor (uPAR) in both tissue and blood are associated with poor survival in several cancer diseases. The prognostic significance of uPAR in cholangiocarcinoma is unknown. The aims of this study were to...

  11. The complex between urokinase (uPA) and its type-1 inhibitor (PAI-1) in pulmonary adenocarcinoma: Relation to prognosis

    Pappot, Helle; Pedersen, Anders N; Brünner, Nils; Christensen, Ib Jarle

    2006-01-01

    In a lung cancer population comprising tumor tissue from 99 pulmonary adenocarcinoma patients, the relationship between tumor tissue level of the complex formed of urokinase (uPA) and its type-1 inhibitor (PAI-1) and survival was studied. The study included patient material previously investigated...... patients with low PAI-1 and high uPA-PAI-1 complex (HR = 3.06, p = 0.01). This is the first investigation of the prognostic impact of uPA-PAI-1 complex in a tumor type other than breast cancer, showing low levels of uPA-PAI-1 complex in combination with high levels of PAI-1 to be associated with poor...... for the prognostic impact of PAI-1 on survival. Standard clinical parameters were available and the patients had a median survival time of 25 months. An ELISA established to measure preformed uPA-PAI-1 complexes was applied to the tumor extracts and previously measured data on uPA and PAI-1 levels...

  12. Comparative study of intraarterial and intravenous thrombolysis with urokinase in canine models of acute pulmonary arterial embolism

    Objective: To evaluate the efficiency and safety of intraarterial thrombolysis with urokinase (UK)in canine models of acute pulmonary arterial embolism. Methods: 24 Canine models of selective acute pulmonary artery embolism with autologous blood clot were divided randomly into 3 groups(each with 8 dogs): intraarterial thrombolytic group (10 000 u/kg of UK infused in 30 rain), intravenous thrombolytic group (20 000 u/kg of UK infused in 2 h) and control group (100 ml of saline infused in 2 h). The value of pulmonary arterial mean pressure (PAMP), blood gas analysis (PaO2 and PaCO2), coagulation index(PT and APTT)and D-dimer were tested at 2 h, 4 h after the thrombolytic procedure and pulmonary arterial angiography was repeated. Results: Comparing the levels of PAMP, PaO2 and D-dimer, showed significant difference in the 3 groups (P2 and blood supply restores more rapidly than the intravenous one. (authors)

  13. Serum levels of soluble urokinase plasminogen activator receptor is associated with parasitemia in children with acute Plasmodium falciparum malaria infection

    Perch, M; Kofoed, Pe; Fischer, Torge; Có, F; Rombo, L; Aaby, P; Eugen-Olsen, J

    2004-01-01

    Serum levels of soluble urokinase plasminogen activator receptor (suPAR) are significantly elevated and of prognostic value in patients suffering from serious infectious diseases such as HIV and tuberculosis. Our objective was to investigate suPAR levels during symptomatic malaria infection and 7...... days after treatment. Children younger than 6 years who presented with fever or other symptoms compatible with malaria were enrolled. Blood films and samples were collected on day 0 and day 7. Twenty-five children were allocated to each of three groups according to the amount of Plasmodium falciparum...... group 1 after 7 days of treatment. All became malaria negative in their blood slides and all decreased in suPAR level to median 3.48 ng/mL (IQR: 3.08-3.91) (P <0.0001). Group 2 consisted of 25 children with 1-20 parasites in their blood slide. The suPAR level was median 2.91 ng/mL (IQR: 2.27-4.40) and...

  14. Catheter-Directed Thrombolysis with a Continuous Infusion of Low-Dose Urokinase for Non-Acute Deep Venous Thrombosis of the Lower Extremity

    Gao, Binbin; Zhang, Jingyong; Wu, Xuejun; Han, Zonglin; Zhou, Hua; Dong, Dianning; Jin, Xing [Shandong Provincial Hospital, Shandong University, Ji' nan (China)

    2011-02-15

    We wanted to evaluate the feasibility of catheter-directed thrombolysis with a continuous infusion of low-dose urokinase for treating non-acute (less than 14 days) deep venous thrombosis of the lower extremity. The clinical data of 110 patients who were treated by catheter-directed thrombolysis with a continuous infusion of low-dose urokinase for lower extremity deep venous thrombosis was analysed. Adjunctive angioplasty or/and stenting was performed for the residual stenosis. Venous recanalization was graded by pre- and posttreatment venography. Follow-up was performed by clinical evaluation and Doppler ultrasound. A total of 112 limbs with deep venous thrombosis with a mean symptom duration of 22.7 days (range: 15-38 days) were treated with a urokinase infusion (mean: 3.5 million IU) for a mean of 196 hours. After thrombolysis, stent placement was performed in 25 iliac vein lesions and percutaneous angioplasty (PTA) alone was done in fi ve iliac veins. Clinically significant recanalization was achieved in 81% (90 of 112) of the treated limbs: complete recanalization was achieved in 28% (31 of 112) and partial recanalization was achieved in 53% (59 of 112). Minor bleeding occurred in 14 (13%) patients, but none of the patients suffered from major bleeding or symptomatic pulmonary embolism. During followup (mean: 15.2 months, range: 3-24 months), the veins were patent in 74 (67%) limbs. Thirty seven limbs (32%) showed progression of the stenosis with luminal narrowing more than 50%, including three with rethrombosis, while one revealed an asymptomatic iliac vein occlusion: 25 limbs (22%) developed mild post-thrombotic syndrome, and none had severe post-thrombotic syndrome. Valvular reflux occurred in 24 (21%) limbs. Catheter-directed thrombolysis with a continuous infusion of low-dose urokinase combined with adjunctive iliac vein stenting is safe and effective for removal of the clot burden and for restoration of the venous flow in patients with non-acute lower

  15. Direct interaction of the kringle domain of urokinase-type plasminogen activator (uPA) and integrin alpha v beta 3 induces signal transduction and enhances plasminogen activation

    Tarui, Takehiko; Akakura, Nobuaki; Majumdar, Mousumi; Andronicos, Nicholas; Takagi, Junichi; Mazar, Andrew P.; Bdeir, Khalil; Kuo, Alice; Yarovoi, Serge V.; Cines, Douglas B.; Takada, Yoshikazu

    2006-01-01

    It has been questioned whether there are receptors for urokinase-type plasminogen activator (uPA) that facilitate plasminogen activation other than the high affinity uPA receptor (uPAR/CD87) since studies of uPAR knockout mice did not support a major role of uPAR in plasminogen activation. uPA also promotes cell adhesion, chemotaxis, and proliferation besides plasminogen activation. These uPA-induced signaling events are not mediated by uPAR, but mediated by unidentified, lower-affinity recep...

  16. Mapping the topographic epitope landscape on the urokinase plasminogen activator receptor (uPAR) by surface plasmon resonance and X-ray crystallography

    Baoyu Zhao; Sonu Gandhi; Cai Yuan; Zhipu Luo; Rui Li; Henrik Gårdsvoll; Valentina de Lorenzi; Nicolai Sidenius; Mingdong Huang; Michael Ploug

    2015-01-01

    The urokinase-type plasminogen activator receptor (uPAR or CD87) is a glycolipid-anchored membrane protein often expressed in the microenvironment of invasive solid cancers and high levels are generally associated with poor patient prognosis (Kriegbaum et al., 2011 [1]). uPAR is organized as a dynamic modular protein structure composed of three homologous Ly6/uPAR domains (LU).This internally flexible protein structure of uPAR enables an allosteric regulation of the interactions with its two ...

  17. Prognostic value analysis of urokinase-type plasminogen activator receptor in oral squamous cell carcinoma: an immunohistochemical study

    Oral squamous cell carcinoma (OSCC) represents the most common oral malignancy. Despite recent advances in therapy, up to 50% of the cases have relapse and/or metastasis. There is therefore a strong need for the identification of new biological markers able to predict the clinical behaviour of these lesions in order to improve quality of life and overall survival. Among tumour progression biomarkers, already known for their involvement in other neoplasia, a crucial role is ascribed to the urokinase-type plasminogen activator receptor (uPAR), which plays a multiple role in extracellular proteolysis, cell migration and tissue remodelling not only as a receptor for the zymogen pro-uPA but also as a component for cell adhesion and as a chemoattractant. The purpose of this study was to gain information on the expression of uPAR in OSCC and to verify whether this molecule can have a role as a prognostic/predictive marker for this neoplasia. In a retrospective study, a cohort of 189 OSCC patients was investigated for uPAR expression and its cellular localization by immunohistochemistry. As standard controls, 8 normal oral mucosal tissues free of malignancy, obtained from patients with no evidence or history of oral cavity tumours, were similarly investigated. After grouping for uPAR expression, OSCCs were statistically analyzed for the variables age, gender, histological grading (G), tumour size, recurrence, TNM staging and overall survival rate. In our immunohistochemical study, 74 cases (39.1%) of OSCC showed a mostly cytoplasmic positivity for uPAR, whereas 115 were negative. uPAR expression correlated with tumour differentiation grade and prognosis: percentage of positive cases was the greatest in G3 (70.4%) and patients positives for uPAR expression had an expectation of life lower than those for uPAR negatives. The results obtained in this study suggest a role of uPAR as a potential biomarker useful to identify higher risk subgroups of OSCC patients

  18. Plasminogen activator inhibitor-1 (PAI-1 and urokinase plasminogen activator (uPA in sputum of allergic asthma patients.

    Sebastian Zukowski

    2008-06-01

    Full Text Available Urokinase plasminogen activator (uPA and its inhibitor (PAI-1 have been associated with asthma. The aim of this study was to evaluate concentration of uPA and PAI-1 in induced sputum of house dust mite allergic asthmatics (HDM-AAs. The study was performed on 19 HDM-AAs and 8 healthy nonatopic controls (HCs. Concentration of uPA and PAI-1 was evaluated in induced sputum supernatants using ELISA method. In HDM-AAs the median sputum concentration of uPA (128 pg/ml; 95% CI 99 to 183 pg/ml and PAI-1 (4063 pg/ml; 95%CI 3319 to 4784 pg/ml were significantly greater than in HCs (17 pg/ml; 95%CI 12 to 32 pg/ml; p<0.001 and 626 pg/ml; 95%CI 357 to 961 pg/ml; p<0.001 for uPA and PAI-1 respectively. The sputum concentration of uPA correlated with sputum total cell count (r=0.781; p=0.0001 and with logarithmically transformed exhaled nitric oxide concentration (eNO (r=0.486; p=0.035 but not with FEV1 or bronchial reactivity to histamine. On the contrary, the sputum PAI-1 concentration correlated with FEV1 (r=-0,718; p=0.0005 and bronchial reactivity to histamine expressed as log(PC20 (r=-0.824; p<0.0001 but did not correlate with sputum total cell count or eNO. The results of this study support previous observations linking PAI-1 with airway remodeling and uPA with cellular inflammation. Moreover, the observed effect of uPA seems to be independent of its fibrynolytic activity.

  19. Proteomic Identification of LASP-1 Down-regulation After RNAi Urokinase Silencing in Human Hepatocellular Carcinoma Cells

    Alessandro Salvi

    2009-02-01

    Full Text Available In human hepatocellular carcinoma (HCC, the high expression of urokinase-type plasminogen activator (uPA is an unfavorable prognostic factor and a therapeutic target. To identify the downstream effects of uPA silencing by RNA interference, we studied proteome modifications of uPA-inhibited SKHep1C3 cells, an HCC-derived cell line. The study with two-dimensional difference gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry showed Lim and SH3 protein 1 (LASP-1, cytokeratin 1 (CK-1, cytokeratin 10 (CK-10, and heterogeneous nuclear ribonucleoprotein H1 down-modulation after uPA inhibition. LASP-1, CK-1, and CK-10 are involved in cytoskeleton dynamics as heterogeneous nuclear ribonucleoprotein H1 takes part in the mRNA processing and stability. We first confirmed the proteomic data by Western blot and immunoflorescence and then explored the link between uPA and LASP-1. The ectopic expression of uPA and LASP-1 supported the proteomic results and showed that uPA up-regulation increased LASP-1 expression and that both were implicated in SKHep1C3 motility. siRNA LASP-1 inhibition showed that LASP-1 was involved in actin microfilaments organization of SKHep1C3 cells. The disruption of the actin microfilaments after LASP-1 depletion increased uPA secretion and SKHep1C3 motility. Our results would suggest the hypothesis that uPA and LASP-1 expression may be coordinated in HCC-derived cells. In summary, the proteomic identification of a set of uPA downstream proteins provides new insight into the function of uPA in HCC cells.

  20. CFTR suppresses tumor progression through miR-193b targeting urokinase plasminogen activator (uPA) in prostate cancer.

    Xie, C; Jiang, X H; Zhang, J T; Sun, T T; Dong, J D; Sanders, A J; Diao, R Y; Wang, Y; Fok, K L; Tsang, L L; Yu, M K; Zhang, X H; Chung, Y W; Ye, L; Zhao, M Y; Guo, J H; Xiao, Z J; Lan, H Y; Ng, C F; Lau, K M; Cai, Z M; Jiang, W G; Chan, H C

    2013-05-01

    Cystic fibrosis (CF) transmembrane conductance regulator (CFTR) is expressed in the epithelial cells of a wide range of organs/tissues from which most cancers are derived. Although accumulating reports have indicated the association of cancer incidence with genetic variations in CFTR gene, the exact role of CFTR in cancer development and the possible underlying mechanism have not been elucidated. Here, we report that CFTR expression is significantly decreased in both prostate cancer cell lines and human prostate cancer tissue samples. Overexpression of CFTR in prostate cancer cell lines suppresses tumor progression (cell growth, adhesion and migration), whereas knockdown of CFTR leads to enhanced malignancies both in vitro and in vivo. In addition, we demonstrate that CFTR knockdown-enhanced cell proliferation, cell invasion and migration are significantly reversed by antibodies against either urokinase plasminogen activator (uPA) or uPA receptor (uPAR), which are known to be involved in various malignant traits of cancer development. More interestingly, overexpression of CFTR suppresses uPA by upregulating the recently described tumor suppressor microRNA-193b (miR-193b), and overexpression of pre-miR-193b significantly reverses CFTR knockdown-enhanced malignant phenotype and abrogates elevated uPA activity in prostate cancer cell line. Finally, we show that CFTR gene transfer results in significant tumor repression in prostate cancer xenografts in vivo. Taken together, the present study has demonstrated a previously undefined tumor-suppressing role of CFTR and its involvement in regulation of miR-193b in prostate cancer development. PMID:22797075

  1. The evaluation of the cardiac function and the effect of urokinase therapy in patients with AMI in ICU

    The cardiac function and the effect of therapy in patients with acute myocardial infarction (AMI) were assessed in 61 patients by analysis of Tl-scintigraphy and Tc-HSA gated pool study in our ICU. Nineteen of them were treated with intravenous Urokinase (UK) within 6 hours from onset. The other 36 patients without UK treatment served as a control group. Significant correlation was recognized between Tl-defect ratio calculated by the circumferential profile curve and peak-CPK levels with a correlation coefficient of 0.578. Significant correlation (r=-0.674) was found between Tl-defect ratio and LVEF. Cardiac function, Tl-defect ratio and peak-CPK levels were compared among patients with antero-septal AMI, inferior AMI and posterior AMI. Significant reductions of LVEF (37.2+-11.7 %) were observed in antero-septal AMI, while RVEF (43.0+-10.0 %) showed significant decreases in inferior AMI. In addition, Tl-defect ratio (3.36+-1.97) and peak-CPK levels in antero-septal AMI were significant higher than those in iferior AMI and posterior AMI. The UK group showed a significant increase of LVEF as compared with the control group in patients with antero-septal AMI. In patients with inferior AMI, no significant differences were observed between UK and control group in LVEF, RVEF and Tl-defect ratio. We have demonstrated that a combination of Tl-scintigraphy and Tc-HSA gated pool study are useful techniques especially when performed in ICU, to evaluate the cardiac function and the effect of thrombolysis therapy and thus greatly contribute to the primary care of AMI cases. (author)

  2. MULTILATERAL DIPLOMACY AND INTERNATIONAL REGIMES

    BENEA Ciprian-Beniamin

    2014-12-01

    Full Text Available The history of diplomacy can by divided in three main periods: one is that of occasional diplomacy peculiar to Middle Ages, while other belong to permane diplomacy, peculiar to modern times. But this one can be divided in two parts, too: one with a bilateral character, previos to 1st World War, and one with a multilateral character, manifested especially after the end on 1st World War. This third type is the focus of present paper. And it cannot be separated from the newly international constructs: international regimes, and international organizations. International instritutions the area where international regimes are belonging to – are legal constructs which provide the formal (and legal framework for continous negotiations. They are the most visible part of the new diplomacy – the one which has a permanent character, and it has an more open face. Anyway, the most important connection has to do with the international institutions, international regimes, and multilateral international negotiations. In the era of the new diplomacy, they all have a permanent character. International institutions help international negotiations carring on; while in their turn, they provide the base for international regimes’ creation, and especially for their evolution. The international regimes’ evolution is an inseparable part of a permanent international framework. And if there is missing a permanent international framework (international organization connected to a specific regime, this regime is a difuse one, its members have only informal relations among them, while they survey each other, looking at their behavior, but they don’t have a formal relationship among them, which could help them solving their future common interests, and protect them from their common fears. International regimes are very important in the era when evrithing touches, and influences everything. In the same time, the complexity of our present world can be successfully

  3. Cloud regimes as phase transitions

    Stechmann, Samuel N.; Hottovy, Scott

    2016-06-01

    Clouds are repeatedly identified as a leading source of uncertainty in future climate predictions. Of particular importance are stratocumulus clouds, which can appear as either (i) closed cells that reflect solar radiation back to space or (ii) open cells that allow solar radiation to reach the Earth's surface. Here we show that these clouds regimes -- open versus closed cells -- fit the paradigm of a phase transition. In addition, this paradigm characterizes pockets of open cells as the interface between the open- and closed-cell regimes, and it identifies shallow cumulus clouds as a regime of higher variability. This behavior can be understood using an idealized model for the dynamics of atmospheric water as a stochastic diffusion process. With this new conceptual viewpoint, ideas from statistical mechanics could potentially be used for understanding uncertainties related to clouds in the climate system and climate predictions.

  4. Inhibitory effect of berberine on the invasion of human lung cancer cells via decreased productions of urokinase-plasminogen activator and matrix metalloproteinase-2

    Berberine, a compound isolated from medicinal herbs, has been reported with many pharmacological effects related to anti-cancer and anti-inflammation capabilities. In this study, we observed that berberine exerted a dose- and time-dependent inhibitory effect on the motility and invasion ability of a highly metastatic A549 cells under non-cytotoxic concentrations. In cancer cell migration and invasion process, matrix-degrading proteinases are required. A549 cell treated with berberine at various concentrations showed reduced ECM proteinases including matrix metalloproteinase-2 (MMP2) and urokinase-plasminogen activator (u-PA) by gelatin and casein zymography analysis. The inhibitory effect is likely to be at the transcriptional level, since the reduction in the transcripts levels was corresponding to the proteins. Moreover, berberine also exerted its action via regulating tissue inhibitor of metalloproteinase-2 (TIMP-2) and urokinase-plasminogen activator inhibitor (PAI). The upstream mediators of the effect involved c-jun, c-fos and NF-κB, as evidenced by reduced phosphorylation of the proteins. These findings suggest that berberine possesses an anti-metastatic effect in non-small lung cancer cell and may, therefore, be helpful in clinical treatment

  5. Urokinase plasminogen receptor and the fibrinolytic complex play a role in nerve repair after nerve crush in mice, and in human neuropathies.

    Cristina Rivellini

    Full Text Available Remodeling of extracellular matrix (ECM is a critical step in peripheral nerve regeneration. In fact, in human neuropathies, endoneurial ECM enriched in fibrin and vitronectin associates with poor regeneration and worse clinical prognosis. Accordingly in animal models, modification of the fibrinolytic complex activity has profound effects on nerve regeneration: high fibrinolytic activity and low levels of fibrin correlate with better nerve regeneration. The urokinase plasminogen receptor (uPAR is a major component of the fibrinolytic complex, and binding to urokinase plasminogen activator (uPA promotes fibrinolysis and cell movement. uPAR is expressed in peripheral nerves, however, little is known on its potential function on nerve development and regeneration. Thus, we investigated uPAR null mice and observed that uPAR is dispensable for nerve development, whereas, loss of uPAR affects nerve regeneration. uPAR null mice showed reduced nerve repair after sciatic nerve crush. This was a consequence of reduced fibrinolytic activity and increased deposition of endoneurial fibrin and vitronectin. Exogenous fibrinolysis in uPAR null mice rescued nerve repair after sciatic nerve crush. Finally, we measured the fibrinolytic activity in sural nerve biopsies from patients with peripheral neuropathies. We showed that neuropathies with defective regeneration had reduced fibrinolytic activity. On the contrary, neuropathies with signs of active regeneration displayed higher fibrinolytic activity. Overall, our results suggest that enforced fibrinolysis may facilitate regeneration and outcome of peripheral neuropathies.

  6. Friction Regimes in the Lubricants Solid-State Regime

    Schipper, D.J.; Maathuis, O.; Dowson, D.; Taylor, C.M.; Childs, T.H.C.; Dalmaz, G.

    1995-01-01

    Friction measurements were performed in the lubricant's solid-state regime to study the transition from full-film lubrication, in which the separation is maintained by a solidified lubricant, to mixed lubrication. Special attention is paid to the influence of temperature (inlet viscosity) and roughn

  7. Postoperative blood loss reduction in computer-assisted surgery total knee replacement by low dose intra-articular tranexamic acid injection together with 2-hour clamp drain: a prospective triple-blinded randomized controlled trial

    Paphon Sa-ngasoongsong

    2011-10-01

    Full Text Available A high-dose local tranexamic acid has been introduced in total knee arthroplasty for bleeding control. We are not sure about the systemic absorption and side effects. The aim of this study was to evaluate the effect of low dosage of intra-articular tranexamic acid injection combined with 2-hour clamp drain in minimally bleeding computer-assisted surgery total knee replacement (CAS-TKR. A prospective randomized controlled trial was conducted in a total of 48 patients underwent CAS-TKR. The patients were randomly assigned to receive either of a mixed intra-articular solution of tranexamic acid 250 mg with physiologic saline (TXA group, or physiologic saline (control group and then followed by clamp drain for 2 hours. Postoperative blood loss was measured by three different methods as drainage volume, total hemoglobin loss and calculated total blood loss. Transfusion requirement and postoperative complications were recorded. All patients were screened for deep vein thrombosis and the functional outcomes were evaluated at 6 months after surgery. The mean postoperative drainage volume, total hemoglobin loss and calculated total blood loss in TXA group were 308.8 mL, 2.1 g/dL and 206.3 mL compared to 529.0 mL, 3.0 g/dL and 385.1 mL in the control group (P=0.0003, 0.0005 and <0.0001 respectively. Allogenic blood transfusion was needed for one patient (4.2% in TXA group and for eight patients (33.3% in the control group. Postoperative knee scores were not significantly different between groups. No deep vein thrombosis, infection or wound complication was detected in both groups. In this study, low dose intra-articular tranexamic acid injection combined with 2-hour clamping drain was effective for reducing postoperative blood loss and transfusion requirement in CAS-TKR without significant difference in postoperative complications or functional outcomes.

  8. Postoperative blood loss reduction in computer-assisted surgery total knee replacement by low dose intra-articular tranexamic acid injection together with 2-hour clamp drain: a prospective triple-blinded randomized controlled trial

    Sa-ngasoongsong, Paphon; Channoom, Thanaphot; Kawinwonggowit, Viroj; Woratanarat, Patarawan; Chanplakorn, Pongsthorn; Wibulpolprasert, Bussanee; Wongsak, Siwadol; Udomsubpayakul, Umaporn; Wechmongkolgorn, Supaporn; Lekpittaya, Nantaporn

    2011-01-01

    A high-dose local tranexamic acid has been introduced in total knee arthroplasty for bleeding control. We are not sure about the systemic absorption and side effects. The aim of this study was to evaluate the effect of low dosage of intra-articular tranexamic acid injection combined with 2-hour clamp drain in minimally bleeding computer-assisted surgery total knee replacement (CAS-TKR). A prospective randomized controlled trial was conducted in a total of 48 patients underwent CAS-TKR. The patients were randomly assigned to receive either of a mixed intra-articular solution of tranexamic acid 250 mg with physiologic saline (TXA group), or physiologic saline (control group) and then followed by clamp drain for 2 hours. Postoperative blood loss was measured by three different methods as drainage volume, total hemoglobin loss and calculated total blood loss. Transfusion requirement and postoperative complications were recorded. All patients were screened for deep vein thrombosis and the functional outcomes were evaluated at 6 months after surgery. The mean postoperative drainage volume, total hemoglobin loss and calculated total blood loss in TXA group were 308.8 mL, 2.1 g/dL and 206.3 mL compared to 529.0 mL, 3.0 g/dL and 385.1 mL in the control group (P=0.0003, 0.0005 and <0.0001 respectively). Allogenic blood transfusion was needed for one patient (4.2%) in TXA group and for eight patients (33.3%) in the control group. Postoperative knee scores were not significantly different between groups. No deep vein thrombosis, infection or wound complication was detected in both groups. In this study, low dose intra-articular tranexamic acid injection combined with 2-hour clamping drain was effective for reducing postoperative blood loss and transfusion requirement in CAS-TKR without significant difference in postoperative complications or functional outcomes. PMID:22053253

  9. On the regimes of premixing

    Angelini, S.; Theofanous, T.G.; Yuen, W.W. [California Univ., Santa Barbara, CA (United States). Center for Risk Studies and Safety

    1998-01-01

    The conditions of the MAGICO-2000 experiment are extended to more broadly investigate the regimes of premixing, and the corresponding internal structures of mixing zones. With the help of the data and numerical simulations using the computer code PM-ALPHA, we can distinguish extremes of behavior dominated by inertia and thermal effects - we name these the inertia and thermal regimes, respectively. This is an important distinction that should guide future experiments aimed at code verification in this area. Interesting intermediate behaviors are also delineated and discussed. (author)

  10. The Choice of Monetary Regime

    Østrup, Finn

    comparisonbetween monetary regimes suggests that welfare is highest under nominalincome targeting where the nominal income target is determined to bring aboutprice stability.Keywords: Monetary regimes; fiscal policy; monetary non-neutrality.JEL classicification: E42, E61, E62.......The article examines how government spending is determined in a closedeconomy where the nominal wage is pre-set through contracts and the wage settershave perfect foresight regarding subsequent policy decisions. The monetaryregime affects government spending because: (i) with a pre-set nominal wage...

  11. Postoperative blood loss reduction in computer-assisted surgery total knee replacement by low dose intra-articular tranexamic acid injection together with 2-hour clamp drain: a prospective triple-blinded randomized controlled trial

    Paphon Sa-ngasoongsong; Thanaphot Channoom; Viroj Kawinwonggowit; Patarawan Woratanarat; Pongsthorn Chanplakorn; Bussanee Wibulpolprasert; Siwadol Wongsak; Umaporn Udomsubpayakul; Supaporn Wechmongkolgorn; Nantaporn Lekpittaya

    2011-01-01

    A high-dose local tranexamic acid has been introduced in total knee arthroplasty for bleeding control. We are not sure about the systemic absorption and side effects. The aim of this study was to evaluate the effect of low dosage of intra-articular tranexamic acid injection combined with 2-hour clamp drain in minimally bleeding computer-assisted surgery total knee replacement (CAS-TKR). A prospective randomized controlled trial was conducted in a total of 48 patients underwent CAS-TKR. The pa...

  12. Nova Scotia's offshore royalty regime

    Oil production from the Hibernia project in Newfoundland's offshore began in December 1997. It is estimated that by 2010 some 30 per cent of Canada's light oil production and as much as 5-10 per cent of its natural gas production will originate from offshore Newfoundland and Nova Scotia. The current level of investment in east coast offshore petroleum developments is the result of the magnitude of discovered and undiscovered petroleum resources in the region. Advances in offshore petroleum technology also contributed to lower development costs. Another reason for increased investment has been an efficient and predictable regulatory environment and government policies. Nova Scotia's royalty regimes and the evolution of royalty regimes for the Sable Offshore Energy project were discussed. The Offshore Petroleum Royalty Act gives Nova Scotia the ability to establish a royalty regime through regulations, or to enter into royalty agreements on a project by project basis, or to use a mix of both agreement and regulation. The philosophy underlying Nova Scotia's royalty regime is to find the right balance between industry and government's share of project revenues

  13. Tumour microenvironments induce expression of urokinase plasminogen activator receptor (uPAR and concomitant activation of gelatinolytic enzymes.

    Synnøve Magnussen

    Full Text Available The urokinase plasminogen activator receptor (uPAR is associated with poor prognosis in oral squamous cell carcinoma (OSCC, and increased expression of uPAR is often found at the invasive tumour front. The aim of the current study was to elucidate the role of uPAR in invasion and metastasis of OSCC, and the effects of various tumour microenvironments in these processes. Furthermore, we wanted to study whether the cells' expression level of uPAR affected the activity of gelatinolytic enzymes.The Plaur gene was both overexpressed and knocked-down in the murine OSCC cell line AT84. Tongue and skin tumours were established in syngeneic mice, and cells were also studied in an ex vivo leiomyoma invasion model. Soluble factors derived from leiomyoma tissue, as well as purified extracellular matrix (ECM proteins, were assessed for their ability to affect uPAR expression, glycosylation and cleavage. Activity of gelatinolytic enzymes in the tissues were assessed by in situ zymography.We found that increased levels of uPAR did not induce tumour invasion or metastasis. However, cells expressing low endogenous levels of uPAR in vitro up-regulated uPAR expression both in tongue, skin and leiomyoma tissue. Various ECM proteins had no effect on uPAR expression, while soluble factors originating from the leiomyoma tissue increased both the expression and glycosylation of uPAR, and possibly also affected the proteolytic processing of uPAR. Tumours with high levels of uPAR, as well as cells invading leiomyoma tissue with up-regulated uPAR expression, all displayed enhanced activity of gelatinolytic enzymes.Although high levels of uPAR are not sufficient to induce invasion and metastasis, the activity of gelatinolytic enzymes was increased. Furthermore, several tumour microenvironments have the capacity to induce up-regulation of uPAR expression, and soluble factors in the tumour microenvironment may have an important role in the regulation of posttranslational

  14. Plasma levels of soluble urokinase-type plasminogen activator receptor associate with the clinical severity of acute Puumala hantavirus infection.

    Tuula K Outinen

    Full Text Available OBJECTIVES: Urokinase-type plasminogen activator receptor is a multifunctional glycoprotein, the expression of which is increased during inflammation. It is known to bind to β3-integrins, which are elementary for the cellular entry of hantaviruses. Plasma soluble form of the receptor (suPAR levels were evaluated as a predictor of severe Puumala hantavirus (PUUV infection and as a possible factor involved in the pathogenesis of the disease. DESIGN: A single-centre prospective cohort study. SUBJECTS AND METHODS: Plasma suPAR levels were measured twice during the acute phase and once during the convalescence in 97 patients with serologically confirmed acute PUUV infection using a commercial enzyme-linked immunosorbent assay (ELISA. RESULTS: The plasma suPAR levels were significantly higher during the acute phase compared to the control values after the hospitalization (median 8.7 ng/ml, range 4.0-18.2 ng/ml vs. median 4.7 ng/ml, range 2.4-12.2 ng/ml, P<0.001. The maximum suPAR levels correlated with several variables reflecting the severity of the disease. There was a positive correlation with maximum leukocyte count (r = 0.475, p<0.001, maximum plasma creatinine concentration (r = 0.378, p<0.001, change in weight during the hospitalization (r = 0.406, p<0.001 and the length of hospitalization (r = 0.325, p = 0.001, and an inverse correlation with minimum platelet count (r = -0.325, p = 0.001 and minimum hematocrit (r = -0.369, p<0.001. CONCLUSION: Plasma suPAR values are markedly increased during acute PUUV infection and associate with the severity of the disease. The overexpression of suPAR possibly activates β3-integrin in PUUV infection, and thus might be involved in the pathogenesis of the disease.

  15. Plasma Levels of Soluble Urokinase-Type Plasminogen Activator Receptor Associate with the Clinical Severity of Acute Puumala Hantavirus Infection

    Outinen, Tuula K.; Tervo, Laura; Mäkelä, Satu; Huttunen, Reetta; Mäenpää, Niina; Huhtala, Heini; Vaheri, Antti; Mustonen, Jukka; Aittoniemi, Janne

    2013-01-01

    Objectives Urokinase-type plasminogen activator receptor is a multifunctional glycoprotein, the expression of which is increased during inflammation. It is known to bind to β3-integrins, which are elementary for the cellular entry of hantaviruses. Plasma soluble form of the receptor (suPAR) levels were evaluated as a predictor of severe Puumala hantavirus (PUUV) infection and as a possible factor involved in the pathogenesis of the disease. Design A single-centre prospective cohort study. Subjects and Methods Plasma suPAR levels were measured twice during the acute phase and once during the convalescence in 97 patients with serologically confirmed acute PUUV infection using a commercial enzyme-linked immunosorbent assay (ELISA). Results The plasma suPAR levels were significantly higher during the acute phase compared to the control values after the hospitalization (median 8.7 ng/ml, range 4.0–18.2 ng/ml vs. median 4.7 ng/ml, range 2.4–12.2 ng/ml, P<0.001). The maximum suPAR levels correlated with several variables reflecting the severity of the disease. There was a positive correlation with maximum leukocyte count (r = 0.475, p<0.001), maximum plasma creatinine concentration (r = 0.378, p<0.001), change in weight during the hospitalization (r = 0.406, p<0.001) and the length of hospitalization (r = 0.325, p = 0.001), and an inverse correlation with minimum platelet count (r = −0.325, p = 0.001) and minimum hematocrit (r = −0.369, p<0.001). Conclusion Plasma suPAR values are markedly increased during acute PUUV infection and associate with the severity of the disease. The overexpression of suPAR possibly activates β3-integrin in PUUV infection, and thus might be involved in the pathogenesis of the disease. PMID:23990945

  16. Role of Urokinase-type Plasminogen Activator in the Precontact Sperm-egg Communication and Fertility of Mice in vitro

    2005-01-01

    Objective To explore the role of urokinase-type plasminogen activator(uPA) in precontact sperm-egg communication and fertility of mice in vitro.Methods Firstly, sperm chemotaxis (SC) induced by uPA was assayed by measuring the sperm densities in capillaries with a descending gradient or no gradient of uPA respectively. Secondly, the role of uPAR that exists in sperm plasma membrane in SC was studied by examining the change of sperm density in capillary after incubating spermatozoa with anti-uPAR antibody. Thirdly, SC induced by eggs, which had been treated with uPA, PAI-1 and anti-uPAR beforehand respectively, was assayed to study the role of uPA in PSEC. Lastly, the fertilization capability of spermatozoa treated with uPA was examined by counting the number of fertilized eggs.Results 1)The density of spermatozoa that migrated down the gradient of uPA into the capillary was significantly lower than that into the capillary containing no-gradient uPA. 2) When uPAR of spermatozoa was inhibited by anti-uPAR antibody, the density of spermatozoa that migrated into the capillary with ascending gradient of uPA decreased correspondingly. 3) The density of spermatozoa attracted by eggs, which were treated with uPA beforehand, increased significantly than that of attracted by non-treated eggs. On the contrary, the sperm density decreased correspondingly when the egg was treated with PAI-1. 4) The number of fertilized eggs increased significantly after the spermatozoa used here was treated with uPA beforehand.Conclusion uPA could induce SC of mice sperm in vitro through the uPAR on its membrane, enhance the capability of egg inducing SC, and promote spermatozoa to fertilize eggs. Thus, uPA may act as an attractant in PSEC, increase the chance encounter of spermatozoa and eggs, therefore, enhance the fertility success correspondingly.This study, in some degree, provides an evidence that uPA may be used as a new medicine and diagnostic reagent for male infertility.

  17. Friction Regimes in the Lubricants Solid-State Regime

    Schipper, D.J.; Maathuis, O.; Dowson, D; Taylor, C.M.; Childs, T.H.C.; Dalmaz, G.

    1995-01-01

    Friction measurements were performed in the lubricant's solid-state regime to study the transition from full-film lubrication, in which the separation is maintained by a solidified lubricant, to mixed lubrication. Special attention is paid to the influence of temperature (inlet viscosity) and roughness on this transition. The friction measurements showed that in the lubricants solid-state region three lubrication modes can be distinguished: A) full-film lubrication; separation is maintained b...

  18. Demystifying optimal dynamic treatment regimes.

    Moodie, Erica E M; Richardson, Thomas S; Stephens, David A

    2007-06-01

    A dynamic regime is a function that takes treatment and covariate history and baseline covariates as inputs and returns a decision to be made. Murphy (2003, Journal of the Royal Statistical Society, Series B 65, 331-366) and Robins (2004, Proceedings of the Second Seattle Symposium on Biostatistics, 189-326) have proposed models and developed semiparametric methods for making inference about the optimal regime in a multi-interval trial that provide clear advantages over traditional parametric approaches. We show that Murphy's model is a special case of Robins's and that the methods are closely related but not equivalent. Interesting features of the methods are highlighted using the Multicenter AIDS Cohort Study and through simulation. PMID:17688497

  19. The Concept of Truth Regime

    Lorna Weir

    2008-07-01

    Full Text Available “Truth regime” is a much used but little theorized concept, with the Foucauldian literature presupposing that truth in modernity is uniformly scientific/quasi-scientific and enhances power. I argue that the forms of truth characteristic of our present are wider than Foucault recognized, their relations to power more various, and their historicity more complex. The truth regime of advanced modernity is characterized by multiple, irreducible truth formulae that co-exist and sometimes vie for dominance. A truth formula stabilizes a network of elements: a relation between representation and presentation (words and things, truth and non-truth, and the place of the subject in discourse. Our contemporary truth regime comprises radically heterogeneous truthful knowledges – science, governance, religion/politics, and common culture – that have distinct histories and relations to power.

  20. The emerging climate change regime

    The emerging climate change regime--with the UN Framework Convention on Climate Change (FCCC) at its core--reflects the substantial uncertainties, high stakes and complicated politics of the greenhouse warming issue. The regime represents a hedging strategy. On the one hand, it treats climate change as a potentially serious problem, and in response, creates a long-term, evolutionary process to encourage further research, promote national planning, increase public awareness, and help create a sense of community among states. But it requires very little by way of substantive--and potentially costly--mitigation or adaptation measures. Although the FCCC parties have agreed to negotiate additional commitments, substantial progress is unlikely without further developments in science, technology, and public opinion. The FCCC encourages such developments, and is capable of evolution and growth, should the political will to take stronger international action emerge. 120 refs., 3 tabs

  1. The EU Commission consultation regime

    Quittkat, Christine; Finke, Barbara

    2008-01-01

    "The present consultation regime of the European Commission is marked by the role the Commission assigns to non-governmental actors or civil society organisations (CSOs). The Commission's documents on its policy of consultation and cooperation with external non-governmental actors reveal that a reflective approach has emerged during the 1980s, referring to a more elaborate concept of 'good governance'. The gradual extension is most noticeable in the change of terminology, from ‘consultation’ ...

  2. Contrasting Monetary Regimes in Africa

    Patrick Honohan; Stephen A. O'Connell

    1997-01-01

    In post-independence sub-Saharan Africa, institutional arrangements for monetary policy have taken a variety of forms, although the historical evolution of many African financial systems has been similar. This paper identifies five different regimes and examines how they evolved over time. It focuses on how the alternative institutional arrangements have influenced the performance of monetary policy under fiscal pressure, and concludes that, although the trend appears to be toward more flexib...

  3. The International Climate Change Regime

    Yamin, Farhana; Depledge, Joanna

    2005-01-01

    Aimed at the increasing number of policy-makers, stakeholders, researchers, and other professionals working on climate change, this volume presents a detailed description and analysis of the international regime established in 1992 to combat the threat of global climate change. It provides a comprehensive accessible guide to a high-profile area of international law and politics, covering not only the obligations and rights of countries, but ongoing climate negotiations as well.

  4. Monetary regimes in open economies

    Korpos, A.

    2006-01-01

    This thesis presents a two-country open economy framework for the analysis of strategic interactions among monetary authorities and wage bargaining institutions. From this perspective, the thesis investigates the economic consequences of replacing flexible and fixed exchange rate regimes with a monetary union, such as the European Economic and Monetary Union. It shows that fixing a currency creates a flatter Phillips curve and increases the aggressiveness of wage demands by labor unions. Comp...

  5. Disclosure regimes and corporate governance

    Wüstemann, Jens

    2004-01-01

    As there is a strong relationship of disclosure regimes and corporate governance the internationally varying distribution of information rights can be interpreted as a function of different systems of corporate governance. A disclosure system comprises all legally recognized information claims that a system of corporate governance or a financial system furnishes financial contracting parties with: Both public disclosure and disclosure via private information channels may serve value-relevant ...

  6. Exchange Rate Regimes and Location

    Ricci, Luca Antonio

    1997-01-01

    This paper investigates the effects of fixed versus flexible exchange rate regimes on location choices of firms and on the degree of specialization of countries. In a two-country two-differentiated-good monetary model, demand, supply, and monetary shocks arise after wages are set and prices are optimally chosen. The exchange rate performs then an adjustment role for firms located in the country relatively specialized in the good they produce, but it constitutes a factor of disturbance for the...

  7. Soluble Urokinase Plasminogen Activator Receptor Level Is an Independent Predictor of the Presence and Severity of Coronary Artery Disease and of Future Adverse Events

    Eapen, Danny J; Manocha, Pankaj; Ghasemzedah, Nima;

    2014-01-01

    INTRODUCTION: Soluble urokinase plasminogen activator receptor (suPAR) is an emerging inflammatory and immune biomarker. Whether suPAR level predicts the presence and the severity of coronary artery disease (CAD), and of incident death and myocardial infarction (MI) in subjects with suspected CAD......, is unknown. METHODS AND RESULTS: We measured plasma suPAR levels in 3367 subjects (67% with CAD) recruited in the Emory Cardiovascular Biobank and followed them for adverse cardiovascular (CV) outcomes of death and MI over a mean 2.1±1.1 years. Presence of angiographic CAD (≥50% stenosis in ≥1...... coronary artery) and its severity were quantitated using the Gensini score. Cox's proportional hazard survival and discrimination analyses were performed with models adjusted for established CV risk factors and C-reactive protein levels. Elevated suPAR levels were independently associated with the presence...

  8. A new tagging system for production of recombinant proteins in Drosophila S2 cells using the third domain of the urokinase receptor

    Gårdsvoll, Henrik; Hansen, Line V; Jørgensen, Thomas J D;

    2007-01-01

    chromatography procedure using the immobilized anti-uPAR monoclonal antibody R2. An optional enterokinase cleavage site is included between the various recombinant proteins and the linker region of the tag, which enables generation of highly pure preparations of tag-free recombinant proteins. Using this system......The use of protein fusion tag technology greatly facilitates detection, expression and purification of recombinant proteins, and the demands for new and more effective systems are therefore expanding. We have used a soluble truncated form of the third domain of the urokinase receptor as a...... convenient C-terminal fusion partner for various recombinant extracellular human proteins used in basic cancer research. The stability of this cystein-rich domain, which structure adopts a three-finger fold, provides an important asset for its applicability as a fusion tag for expression of recombinant...

  9. Soluble urokinase plasminogen activator receptor (suPAR) is a novel, independent predictive marker of myocardial infarction in HIV-1-infected patients

    Rasmussen, Line Jee Hartmann; Knudsen, A; Katzenstein, T L;

    2016-01-01

    OBJECTIVES: Patients infected with HIV are at increased risk of myocardial infarction (MI). Increased plasma levels of the inflammatory biomarker soluble urokinase plasminogen activator receptor (suPAR) have been associated with increased risk of cardiovascular diseases (CVD), including MI in the......, gender, duration of antiretroviral therapy (ART), smoking and no known CVD. suPAR was measured in the four plasma samples available for each patient at different time-points; 1, Before initiation of ART; 2, 3 months after initiation of ART; 3, 1 year before the case's MI; and 4, The last sample available...... before the case's MI. RESULTS: In unadjusted conditional regression analysis, higher levels of suPAR were associated with a significant increase in risk of MI at all time-points. Patients in the third and fourth suPAR quartiles had a three- to 10-fold higher risk of MI compared to patients in the lowest...

  10. Plasma soluble urokinase-type plasminogen activator receptor level is independently associated with coronary microvascular function in patients with non-obstructive coronary artery disease

    Mekonnen, Girum; Corban, Michel T; Hung, Olivia Y;

    2015-01-01

    BACKGROUND: Soluble urokinase-type plasminogen activator receptor (suPAR) is a novel biomarker released from leukocytes and endothelial cells that has been associated with atherosclerotic cardiovascular disease. We hypothesized that plasma suPAR level is an independent predictor of coronary...... microvascular function. METHODS: Coronary blood flow velocity and plasma suPAR levels were evaluated in patients with non-obstructive coronary artery disease. Coronary flow reserve (CFR) was calculated as the ratio of hyperemic to basal average peak blood flow velocity and coronary microvascular dysfunction was...... defined as CFR ≤ 2.0 in the setting of a fractional flow reserve value of ≥0.75. Plasma suPAR levels were measured using ELISA technique. The association between suPAR and CFR was investigated using univariate and multivariate regression analyses. RESULTS: In 66 patients, 47% were men, 26% had diabetes...

  11. Presence of urokinase plasminogen activator, its inhibitor and receptor in small cell lung cancer and non-small cell lung cancer

    Pappot, H.; Pfeiffer, P.; Grøndahl Hansen, J.;

    1997-01-01

    Spreading of cancer cells is dependent on the combined action of several proteolytic enzymes, such as serine proteases, comprising the urokinase pathway of plasminogen activation. Previous studies of lung cancer indicate that expression, localization and prognostic impact of the components of the...... plasminogen activation system differ in the different non-small cell lung cancer (NSCLC) types, whereas the expression of the components in small cell lung cancer (SCLC) has only sparingly been investigated. In the present study we investigate the presence of the components of the plasminogen activation...... the clinical parameters. This is the first report of a study using a quantitative method to compare levels of the components of the plasminogen activation system in tissue extracts from the two major lung cancer groups. The study shows that uPA, PAI-1 and uPAR are present in SCLC-tissue, suggesting...

  12. Targeting tumor cell invasion and dissemination in vivo by an aptamer that inhibits urokinase-type plasminogen activator through a novel multifunctional mechanism

    Botkjaer, Kenneth A; Deryugina, Elena I; Dupont, Daniel Miotto;

    2012-01-01

    Data accumulated over the latest two decades have established that the serine protease urokinase-type plasminogen activator (uPA) is a potential therapeutic target in cancer. When designing inhibitors of the proteolytic activity of serine proteases, obtaining sufficient specificity is problematic......, because the topology of the proteases' active sites are highly similar. In an effort to generate highly specific uPA inhibitors with new inhibitory modalities, we isolated uPA-binding RNA aptamers by screening a library of 35 nucleotides long 2'-fluoro-pyrimidine RNA molecules using a version of human pro......-uPA lacking the epidermal growth factor-like and kringle domains as bait. One pro-uPA-binding aptamer sequence, referred to as upanap-126, proved to be highly specific for human uPA. Upanap-126 delayed the proteolytic conversion of human pro-uPA to active uPA, but did not inhibit plasminogen activation...

  13. Distinctive binding modes and inhibitory mechanisms of two peptidic inhibitors of urokinase-type plasminogen activator with isomeric P1 residues

    Jiang, Longguang; Zhao, Baoyu; Xu, Peng;

    2015-01-01

    Two isomeric piperidine derivatives (meta and para isomers) were used as arginine mimics in the P1 position of a cyclic peptidic inhibitor (CPAYSRYLDC) of urokinase-type plasminogen activator. The two resulting cyclic peptides showed vastly different affinities (~70 fold) to the target enzyme. X......-ray crystal structure analysis showed that the two P1 residues were inserted into the S1 specificity pocket in indistinguishable manners. However, the rest of the peptides bound in entirely different ways on the surface of the enzyme, and the two peptides have different conformations, despite the highly...... similar sequence. These results demonstrate how the subtle difference in P1 residue can dictate the exosite interactions and the potencies of peptidic inhibitors, and highlight the importance of P1 residue for protease inhibition. This study provides important information for the development of peptidic...

  14. Anti-Urokinase Receptor Antisense Oligonucleotide (uPAR-aODN) to Prevent and Cure Long-Term Space Exploration-Related Retinal Pathological Angiogenesis

    Lazzarano, Stefano; Lulli, Matteo; Fibbi, Gabriella; Margheri, Francesca; Papucci, Laura; Serrati, Simona; Witort, Ewa; Chilla, Anastasia; Lapucci, Andrea; Donnini, Martino; Quaglierini, Paolo; Romiti, Alice; Specogna, Rebecca; Del Rosso, Mario; Capaccioli, Sergio

    2008-06-01

    Angiogenesis underlies a variety of physiological processes and its possible deregulation during long term space exploration needs to be investigated. Angiogenesis is a multistep process of new blood capillary formation, where degradation of the extracellular matrix (ECM) by proteolytic enzymes, including uPA (urokinase plasminogen activator) and opening the way to migration of endothelial cells (EC), is critical. Plasminogen activation system regulates angiogenesis by both uPA-driven ECM degradation and uPA receptor (uPAR). Microgravity and low dose irradiations promote tissue neoangiogeenesis and neovascularization is often common occurence in ophthalmologic pathologies. We have designed and patented the uPAR antisense oligonucleotide (aODN) and evaluated its antiangiogenetic activity by EC cellular migration and capillary morphogenesis assays. The uPAR aODN treatment caused a 75% inhibition of human microvascular EC migration and a complete inhibition of capillary morphogenesis, suggesting its therapeutic application to prevent neoangiogenesis-related ophthalmologic pathologies during space exploration.

  15. Gene expression of fibrinolytic factors urokinase plasminogen activator and plasminogen activator inhibitor-1 in rabbit temporo-mandibular joint cartilage with disc displacement

    ZHAN Jing; GU Zhi-yuan; WU Li-qun; ZHANG Yin-kai; HU Ji-an

    2005-01-01

    Background The urokinase plasminogen activator system is believed to play an important role in degradation of the extracellular matrix associated with cartilage and bone destruction; however its precise roles in temporomandibular disorders have not yet been clarified. The aims of this study were to investigate the gene expression of fibrinolytic factors urokinase plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) in the articular cartilage of rabbit temporomandibular joint (TMJ) with disc displacement (DD) and to probe the relationship between fibrinolytic activity and cartilage remodeling. Methods Disc displacement of right joints was performed in 36 of 78 rabbits under investigation. The animals were sacrificed at 4 days and 1, 2, 4, 8 and 12 weeks after surgery, respectively. The right joints of these animals were harvested and processed for the examination of mRNA expression of uPA and PAI-1 in articular cartilage using in situ hybridization techniques. Results The expression of uPA and PAI-1 was co-expressed weakly in the chondrocytes from transitive zone to hypertrophic zone and mineralized zone, while no hybridizing signals were shown in proliferative zone and superficial zone in control rabbits. The most striking was the up-regulation of uPA and PAI-1 mRNA in 4-day rabbits postoperatively at the onset of cartilage degeneration. The strongest hybridizing signals for uPA and PAI-1 were seen in 2-week rabbits postoperatively. After 2 weeks, the expression of uPA and PAI-1 began to decrease and reached nearly normal level at 12 weeks. Conclusions The expression of the uPA/PAI-1 system coincides with the pathological changes in condylar cartilage after DD. The uPA/PAI-1 system may be one of the essential mediators in articular cartilage remodeling.

  16. Bacterial endotoxin enhances colorectal cancer cell adhesion and invasion through TLR-4 and NF-kappaB-dependent activation of the urokinase plasminogen activator system.

    Killeen, S D

    2009-05-19

    Perioperative exposure to lipopolysaccharide (LPS) is associated with accelerated metastatic colorectal tumour growth. LPS directly affects cells through Toll-like receptor 4 (TLR-4) and the transcription factor NF-kappaB. The urokinase plasminogen activator (u-PA) system is intimately implicated in tumour cell extracellular matrix (ECM) interactions fundamental to tumour progression. Thus we sought to determine if LPS directly induces accelerated tumour cell ECM adhesion and invasion through activation of the u-PA system and to elucidate the cellular pathways involved. Human colorectal tumour cell lines were stimulated with LPS. u-PA concentration, u-PA activity, active u-PA, surface urokinase plasminogen activator receptor (u-PAR) and TLR-4 expression were assessed by ELISA, colorimetric assay, western blot analysis and flow cytometry respectively. In vitro tumour cell vitronectin adhesion and ECM invasion were analysed by vitronectin adhesion assay and ECM invasion chambers. u-PA and u-PAR function was inhibited with anti u-PA antibodies or the selective u-PA inhibitors amiloride or WXC-340, TLR-4 by TLR-4-blocking antibodies and NF-kappaB by the selective NF-kappaB inhibitor SN-50. LPS upregulates u-PA and u-PAR in a dose-dependent manner, enhancing in vitro tumour cell vitronectin adhesion and ECM invasion by >40% (P<0.01). These effects were ameliorated by u-PA and u-PAR inhibition. LPS activates NF-kappaB through TLR-4. TLR-4 and NF-kappaB inhibition ameliorated LPS-enhanced u-PA and u-PAR expression, tumour cell vitronectin adhesion and ECM invasion. LPS promotes tumour cell ECM adhesion and invasion through activation of the u-PA system in a TLR-4- and NF-kappaB-dependent manner.

  17. Urokinase-type Plasminogen Activator-like Proteases in Teleosts Lack Genuine Receptor-binding Epidermal Growth Factor-like Domains*

    Bager, René; Kristensen, Thomas K.; Jensen, Jan K.; Szczur, Agnieszka; Christensen, Anni; Andersen, Lisbeth M.; Johansen, Jesper S.; Larsen, Niels; Baatrup, Erik; Huang, Mingdong; Ploug, Michael; Andreasen, Peter A.

    2012-01-01

    Plasminogen activation catalyzed by urokinase-type plasminogen activator (uPA) plays an important role in normal and pathological tissue remodeling processes. Since its discovery in the mid-1980s, the cell membrane-anchored urokinase-type plasminogen activator receptor (uPAR) has been believed to be central to the functions of uPA, as uPA-catalyzed plasminogen activation activity appeared to be confined to cell surfaces through the binding of uPA to uPAR. However, a functional uPAR has so far only been identified in mammals. We have now cloned, recombinantly produced, and characterized two zebrafish proteases, zfuPA-a and zfuPA-b, which by several criteria are the fish orthologs of mammalian uPA. Thus, both proteases catalyze the activation of fish plasminogen efficiently and both proteases are inhibited rapidly by plasminogen activator inhibitor-1 (PAI-1). But zfuPA-a differs from mammalian uPA by lacking the exon encoding the uPAR-binding epidermal growth factor-like domain; zfuPA-b differs from mammalian uPA by lacking two cysteines of the epidermal growth factor-like domain and a uPAR-binding sequence comparable with that found in mammalian uPA. Accordingly, no zfuPA-b binding activity could be found in fish white blood cells or fish cell lines. We therefore propose that the current consensus of uPA-catalyzed plasminogen activation taking place on cell surfaces, derived from observations with mammals, is too narrow. Fish uPAs appear incapable of receptor binding in the manner known from mammals and uPA-catalyzed plasminogen activation in fish may occur mainly in solution. Studies with nonmammalian vertebrate species are needed to obtain a comprehensive understanding of the mechanism of plasminogen activation. PMID:22733817

  18. Transcriptional Regulation of Urokinase-type Plasminogen Activator Receptor by Hypoxia-Inducible Factor 1 Is Crucial for Invasion of Pancreatic and Liver Cancer

    Peter Büchler

    2009-02-01

    Full Text Available Angioinvasion is critical for metastasis with urokinase-type plasminogen activator receptor (uPAR and tumor hypoxia-activated hypoxia-inducible factor 1 (HIF-1 as key players. Transcriptional control of uPAR expression by HIF has never been reported. The aim of the present study, therefore, was to test whether tumor hypoxia-induced HIF expression may be linked to transcriptional activation of uPAR and dependent angioinvasion. We used human pancreatic cancer cells and a model of parental and derived HIF-1β-deficient mouse liver cancer cell lines and performed Northern blot analysis, nuclear runoff assays, electrophoretic mobility shift assay, polymerase chain reaction-generated deletion mutants, luciferase assays, Matrigel invasion assays, and in vivo angioinvasion assays in the chorioallantoic membrane of fertilized chicken eggs. Urokinase-type plasminogen activator receptor promoter analysis resulted in four putative HIF binding sites. Hypoxia strongly induced de novo transcription of uPAR mRNA. With sequential deletion mutants of the uPAR promoter, it was possible to identify one HIF binding site causing a nearly 200-fold increase in luciferase activity. Hypoxia enhanced the number of invading tumor cells in vitro and in vivo. In contrast, HIF-1β-deficient cells failed to upregulate uPAR expression, to activate luciferase activity, and to invade on hypoxia. Taken together, we show for the first time that uPAR is under transcriptional control of HIF and that this is important for hypoxia-induced metastasis.

  19. Mapping the topographic epitope landscape on the urokinase plasminogen activator receptor (uPAR by surface plasmon resonance and X-ray crystallography

    Baoyu Zhao

    2015-12-01

    Full Text Available The urokinase-type plasminogen activator receptor (uPAR or CD87 is a glycolipid-anchored membrane protein often expressed in the microenvironment of invasive solid cancers and high levels are generally associated with poor patient prognosis (Kriegbaum et al., 2011 [1]. uPAR is organized as a dynamic modular protein structure composed of three homologous Ly6/uPAR domains (LU.This internally flexible protein structure of uPAR enables an allosteric regulation of the interactions with its two principal ligands: the serine protease urokinase-type plasminogen activator (uPA and the provisional matrix protein vitronectin (Vn (Mertens et al., 2012; Gårdsvoll et al., 2011; Madsen et al., 2007 [2–4]. The data presented here relates to the non-covalent trapping of one of these biologically relevant uPAR-conformations by a novel class of monoclonal antibodies (Zhao et al., 2015 [5] and to the general mapping of the topographic epitope landscape on uPAR. The methods required to achieve these data include: (1 recombinant expression and purification of a uPAR-hybrid protein trapped in the desired conformation [patent; WO 2013/020898 A12013]; (2 developing monoclonal antibodies with unique specificities using this protein as antigen; (3 mapping the functional epitope on uPAR for these mAbs by surface plasmon resonance with a complete library of purified single-site uPAR mutants (Zhao et al., 2015; Gårdsvoll et al., 2006 [5,6]; and finally (4 solving the three-dimensional structures for one of these mAbs by X-ray crystallography alone and in complex with uPAR [deposited in the PDB database as 4QTH and 4QTI, respectively].

  20. Mapping the topographic epitope landscape on the urokinase plasminogen activator receptor (uPAR) by surface plasmon resonance and X-ray crystallography.

    Zhao, Baoyu; Gandhi, Sonu; Yuan, Cai; Luo, Zhipu; Li, Rui; Gårdsvoll, Henrik; de Lorenzi, Valentina; Sidenius, Nicolai; Huang, Mingdong; Ploug, Michael

    2015-12-01

    The urokinase-type plasminogen activator receptor (uPAR or CD87) is a glycolipid-anchored membrane protein often expressed in the microenvironment of invasive solid cancers and high levels are generally associated with poor patient prognosis (Kriegbaum et al., 2011 [1]). uPAR is organized as a dynamic modular protein structure composed of three homologous Ly6/uPAR domains (LU).This internally flexible protein structure of uPAR enables an allosteric regulation of the interactions with its two principal ligands: the serine protease urokinase-type plasminogen activator (uPA) and the provisional matrix protein vitronectin (Vn) (Mertens et al., 2012; Gårdsvoll et al., 2011; Madsen et al., 2007 [2-4]). The data presented here relates to the non-covalent trapping of one of these biologically relevant uPAR-conformations by a novel class of monoclonal antibodies (Zhao et al., 2015 [5]) and to the general mapping of the topographic epitope landscape on uPAR. The methods required to achieve these data include: (1) recombinant expression and purification of a uPAR-hybrid protein trapped in the desired conformation [patent; WO 2013/020898 A12013]; (2) developing monoclonal antibodies with unique specificities using this protein as antigen; (3) mapping the functional epitope on uPAR for these mAbs by surface plasmon resonance with a complete library of purified single-site uPAR mutants (Zhao et al., 2015; Gårdsvoll et al., 2006 [5,6]); and finally (4) solving the three-dimensional structures for one of these mAbs by X-ray crystallography alone and in complex with uPAR [deposited in the PDB database as 4QTH and 4QTI, respectively]. PMID:26504891

  1. Alberta oil sands royalty regime

    The long term objective of the Oil Sands Business Unit of Alberta Energy is to pave the way for Alberta's bitumen production to reach 3 million barrels per day by 2020. This presentation described the national government's role in resource development. It was emphasized that since the Crown is the owner of the oil sands resource, it would benefit by providing strategic leadership and by generating a larger royalty base. The oil sands fiscal regime was described with reference to generic royalty, risk sharing, investment, and project economics. Business rule principles were also outlined along with criteria for project expansions. Both upstream and downstream challenges and opportunities were listed. 4 figs

  2. Visibility regimes in mediatized publicness

    Samuel Mateus

    2014-12-01

    Full Text Available Considering the relationship between politics, media and publicness, this paper ponders the consequences of visibility in the political field. Identifying some of its existing regimes, it will posit that today visibility plays an ambivalent function to politics: it can simultaneously operate as a synoptic monitoring and control of politicians; and at the same time it may stand as an opportunity to build a charismatic leadership. In fact, political visibilities are now negotiated between the boundaries of private and public realms, and they can take the form of a risk, or an opportunity to build on a charismatic leadership.

  3. Twentieth century monetary regimes in Canadian perspective

    Harley, C. Knick

    2001-01-01

    Three monetary regimes - the gold standard, a regime of 'socialism in many countries' and the post-Bretton Woods regime - and difficult transitions between them have shaped the economic history of the twentieth century. The regimes consisted of coherent sets of government policies and equally importantly, the publics expectations about policy and each made different decisions about which of the trilemma of policy goals - fixed exchange rates, domestic policy independence and integrated capita...

  4. Accommodating human values in the climate regime

    Cook, Rosalind; Tauschinsky, Eljalill

    2008-01-01

    The climate regime addresses one of the most important challenges facing humankind today. However, while the environmental and economic sides of the problem are well represented, it lacks the inclusion of social and human aspects. The human rights regime, in contrast, is a regime which has been esta

  5. Measuring the effectiveness of international environmental regimes

    Helm, C.; Sprinz, D.F.

    1999-05-01

    While past research has emphasized the importance of international regimes for international governance, systematic assessments of regime effects are missing. This article derives a standardized measurement concept for the effectiveness of international environmental regimes by developing an operational rational choice calculus to evaluate actual policy simultaneously against a non-regime counterfactual and a collective optimum. Subsequently, the empirical feasibility of the measurement instrument is demonstrated by way of two international treaties regulating transboundary air pollution in Europe. The results demonstrate that the regimes indeed show positive effects - but fall substantially short of the collective optima. (orig.)

  6. Regime Jumps in Electricity Prices

    Electricity prices are known to be very volatile and subject to frequent jumps due to system breakdown, demand shocks, and inelastic supply. As many international electricity markets are in some state of deregulation, more and more participants in these markets are exposed to these stylised facts. Appropriate pricing, portfolio, and risk management models should incorporate these facts. Authors have introduced stochastic jump processes to deal with the jumps, but we argue and show that this specification might lead to problems with identifying the true mean-reversion within the process. Instead, we propose using a regime jump model that disentangles mean-reversion from jump behaviour. This model resembles more closely the true price path of electricity prices

  7. Propagation Regime of Iron Dust Flames

    Tang, Francois-David; Goroshin, Samuel; Higgins, Andrew J.

    2012-01-01

    A flame propagating through an iron-dust mixture can propagate in two asymptotic regimes. When the characteristic time of heat transfer between particles is much smaller than the characteristic time of particle combustion, the flame propagates in the continuum regime where the heat released by reacting particles can be modelled as a space-averaged function. In contrast, when the characteristic time of heat transfer is much larger than the particle reaction time, the flame can no longer be treated as a continuum due to dominating effects associated with the discrete nature of the particle reaction. The discrete regime is characterized by weak dependence of the flame speed on the oxygen concentration compared to the continuum regime. The discrete regime is observed in flames propagating through an iron dust cloud within a gas mixture containing xenon, while the continuum regime is obtained when xenon is substituted with helium.

  8. Accommodating human values in the climate regime

    Rosalind Cook

    2008-12-01

    Full Text Available The climate regime addresses one of the most important challenges facing humankind today. However, while the environmental and economic sides of the problem are well represented, it lacks the inclusion of social and human aspects. The human rights regime, in contrast, is a regime which has been established precisely to implement human values. This article ex-plains the problems of climate change in terms of human values and argues that some proce-dures from the human rights regime offer possibilities for improvement. It is submitted that through the inclusion of human rights instruments, such as individual communication, pro-gressive realisation and authoritative interpretation, the inclusion of human values into the climate regime will be facilitated. This article presents these instruments and discusses their potential for inclusion in the climate regime.

  9. US Forest Service LANDFIRE Historical Fire Regimes

    US Forest Service, Department of Agriculture — Historical fire regimes, intervals, and vegetation conditions are mapped using the Vegetation Dynamics Development Tool (VDDT). These data support fire and...

  10. Crustal stress regime in Italy

    M. Cesaro

    1997-06-01

    Full Text Available In order to obtain a reliable map of the present-day stress field in Italy, needed to better understand the active tectonic processes and to contribute to the assessment of seismic hazard, in 1992 we started to collect and analyze new data from borehole breakouts in deep oil and geothermal wells and focal mechanisms of earthquakes (2.5 < M <5 occurred in Italy between 1988 and 1995. From about 200 deep wells and 300 focal mechanisms analyzed to date, we infer that: the internal (SW sector of the Northern Apenninic arc is extending with minimum compressional stress (Shmin oriented ? ENE, while the external front is thrusting over the Adriatic foreland (Shmin ? NW-SE. The entire Southern Apennine is extending in NE direction (from the Tyrrhenian margin to the Apulian foreland and compression (in the foredeep is no longer active at the outer (NE thrust front. Between these two arcs, an abrupt change in the tectonic regime is detected with directions of horizontal stress changing by as much as 90º in the external front, around latitude 430N. Along the Ionian side of the Calabrian arc the stress directions inferred from breakouts and focal mechanisms are scattered with a hint of rotation from N-S Shmin close to the Southern Apennines, to ~ E-W directions in the Messina Strait. In Sicily, a NW-SE direction of SHmax is evident in the Hyblean foreland, parallel to the direction of plate motion between Africa and Europe. A more complex pattern of stress directions is observed in the thrust belt zone, with rotations from the regional trend (NW í directed SHmax to NE oriented SHmax. A predominant NW direction of SHmax is also detected in mainland Sicily from earthquake focal mechanisms, but no well data are available in this region. In the northern part of Sicily (Aeolian Islands a ~N-S direction of SHmax is observed.

  11. Short-Term Catheter-Directed Thrombolysis with Low-Dose Urokinase Followed by Aspiration Thrombectomy for Treatment of Symptomatic Lower Extremity Deep Venous Thrombosis

    Jung, Se Hee; Lim, Nam Yeul; Song, Jang Hyeon [Dept. of Radiology, Chonnam National University Hospital, Gwangju (Korea, Republic of); Kim, Jae Kyu; Lim, Jae Hoon [Dept. of Radiology, Hospital, Ulsan University School of Medicine, Gweangju (Korea, Republic of); Chang, Nam Kyu [Dept. of Radiology, Chonnam National University Hwasun Hospital, Hwasun (Korea, Republic of); Choi, Soo Jin Na; Chung, Sang Young [Dept. of Radiology, Chonnam National University Hospital, Chonnam National University School of Medicine, Gwangju (Korea, Republic of)

    2011-10-15

    To evaluate the venous patency in patients treated by catheter-directed thrombolysis with low-dose urokinase (UK) for symptomatic lower extremity deep venous thrombosis (DVT). Eighty-nine consecutive patients (46 women and 43 men; mean age, 58.1 years), treated by catheter-directed thrombolysis with low-dose UK were included in this study. Immediate venous patency was evaluated in terms of technical success (successful restoration of antegrade in-line flow in the treated vein with residual stenosis rate of less than 30%) and clinical success (significant reduction of clinical symptoms before hospital discharge). Late venous patency was evaluated in terms of primary patency rate and clinical success. Immediate technical success was achieved in all patients and immediate clinical success in 80 (90%) patients. There was no major systemic bleeding complication. The primary patency rate at 6 months and 12 months was 84% and 79%, respectively. Fifty-six (63%) patients were asymptomatic after a median clinical follow-up of 18 months, eleven (12%) patients improved moderately, seven (8%) patients remained unchanged, and fifteen (17%) patients had no clinical follow-up. Short-term catheter-directed thrombolysis with low-dose UK can be an effective, safe method to manage DVT of the lower extremities.

  12. Short-Term Catheter-Directed Thrombolysis with Low-Dose Urokinase Followed by Aspiration Thrombectomy for Treatment of Symptomatic Lower Extremity Deep Venous Thrombosis

    To evaluate the venous patency in patients treated by catheter-directed thrombolysis with low-dose urokinase (UK) for symptomatic lower extremity deep venous thrombosis (DVT). Eighty-nine consecutive patients (46 women and 43 men; mean age, 58.1 years), treated by catheter-directed thrombolysis with low-dose UK were included in this study. Immediate venous patency was evaluated in terms of technical success (successful restoration of antegrade in-line flow in the treated vein with residual stenosis rate of less than 30%) and clinical success (significant reduction of clinical symptoms before hospital discharge). Late venous patency was evaluated in terms of primary patency rate and clinical success. Immediate technical success was achieved in all patients and immediate clinical success in 80 (90%) patients. There was no major systemic bleeding complication. The primary patency rate at 6 months and 12 months was 84% and 79%, respectively. Fifty-six (63%) patients were asymptomatic after a median clinical follow-up of 18 months, eleven (12%) patients improved moderately, seven (8%) patients remained unchanged, and fifteen (17%) patients had no clinical follow-up. Short-term catheter-directed thrombolysis with low-dose UK can be an effective, safe method to manage DVT of the lower extremities.

  13. Distribution of trichloroacetic acid precipitated radioactivity in rabbits after intravenous injection of 125I-labelled single-chain human recombinant urokinase-type plasminogen activator

    Objective: To study tissue distribution profiles of trichloroacetic acid (TCA) precipitated radioactivity in rabbits following intravenous injection of 125I-labelled single-chain human recombinant urokinase-type plasminogen activator (125I-rh-sc-uPA). Methods: 125I-rh-su-uPA was prepared by Iodogen method and was purified by Saphacryl HR S-200. The radio-chemical purity was measured by reverse phase high performance liquid chromatography (RP-HPLC). Biological activity of labelled protein was measured by thrombolytic activity on fibrin plate. Results: The identification of 125I-rh-sc-uPA revealed that it was a 94.4% radio-chemically purified thrombolytic active protein. The AUC of radioactivity gradients in tissues and other specimens were as follows: kidney>urine>plasma>liver>spleen>adrenal gland>bone marrow>heart>urinary bladder>lungs>gonad>small intestinal wall>fat>lymph node>gall bladder>thymus gland>intestinal content>muscle>brain>feces in colon. Conclusion: The highest TCA precipitated radioactivity was found in plasma following I.V. of 125I-rh-u-PA, which might be favorable for the drug action. The main excretion route was urinary system

  14. The diagnostic value of soluble urokinase plasminogen activator receptor compared with C-reactive protein and procalcitonin in children with febrile neutropenia.

    Sirinoglu, Melis; Soysal, Ahmet; Karaaslan, Ayşe; Kepenekli Kadayifci, Eda; Cinel, Ismail; Koç, Ahmet; Tokuç, Gülnur; Yaman, Ali; Haklar, Goncagül; Şirikçi, Önder; Turan, Serap; Altınkanat Gelmez, Gülşen; Söyletir, Güner; Bakır, Mustafa

    2016-04-01

    The aim of the present study was to determine the diagnostic value of soluble urokinase plasminogen activator receptor (suPAR) in pediatric patients with febrile neutropenia. A prospective case-control study was performed. Patients included 29 children with febrile neutropenia (FN) and 27 control subjects without any infection or immunosuppressive condition. Blood samples were obtained on the day of admission and on the 4th to 7th days of the hospital stay. The median (minimum-maximum) serum levels of suPAR obtained on the first day of the admission were 2.08 (0.93-9.42) and 2.22 (1.08-5.13) ng/mL for the FN group and the control group, respectively. The median serum levels of suPAR in the FN and control groups were not significantly different (P = .053). The mean serum suPAR level was significantly higher in nonsurvivors than in survivors in the FN group (P < .05). In the FN group, the area under the receiver operating characteristics curve (AUCROC) for suPAR was 0.546, but no optimum cutoff value, sensitivity, specificity, negative predictive value (NPV), or positive predictive value (PPV) was obtained. We conclude that suPAR is not useful as a diagnostic biomarker in children with febrile neutropenia; however, persistent high serum suPAR level may predict mortality in FN in children. PMID:27057782

  15. Camptothecin induces urokinase-type plasminogen activator gene-expression in human RC-K8 malignant lymphoma and H69 small cell lung cancer cells.

    Shibakura M

    2002-10-01

    Full Text Available We previously reported that anthracyclines, which could generate reactive oxygen species (ROS, could induce the urokinase-type plasminogen activator (uPA gene expression in human RC-K8 malignant lymphoma cells and in H69 small cell lung cancer (SCLC cells. In screening other uPA-inducible anti-cancer agents, we found that camptothecin (CPT and its derivative, SN38, could induce uPA in RC-K8 and H69 cells. CPT and SN38, which are also used for the treatment of lymphoma and SCLC, significantly increased the uPA accumulation in the conditioned media of both cells in a dose-dependent manner. The maximum induction of uPA mRNA levels was observed 24 h after stimulation. Pretreatment with pyrrolidine dithiocarbamate (PDTC, an anti-oxidant, inhibited the CPT-induced uPA mRNA expression. Thus, CPT induces uPA through gene expression, and, therefore, CPT may influence the tumor-cell biology by up-regulating the uPA/plasmin system.

  16. Extracellular Matrix Biomarker, Fibulin-1, Is Closely Related to NT-proBNP and Soluble Urokinase Plasminogen Activator Receptor in Patients with Aortic Valve Stenosis (The SEAS Study)

    Kruger, Ruan; Rasmussen, Lars M; Argraves, William S;

    2014-01-01

    BACKGROUND: Fibulin-1, a circulating extracellular matrix glycoprotein, has been associated with arterial disease and elevated N-terminal prohormone B-type natriuretic peptide (NT-proBNP) in diabetes. Soluble urokinase plasminogen activator receptor (suPAR), a marker of inflammation, has been...... associated with subclinical atherosclerosis. Therefore, we aimed to explore the interplay between these biomarkers and mild to moderate aortic valve stenosis (AS). METHODS: In 374 patients with mild to moderate AS, we investigated the relationship of fibulin-1 with NT-proBNP, levels of suPAR and the degree...... of AS at baseline and after one and four years of treatment with Simvastatin 40 mg and Ezetimibe 10 mg or placebo. RESULTS: During treatment, fibulin-1 became more closely associated with NT-proBNP (βyear0 = 0.10, p = 0.08, βyear1 = 0.16, p = 0.005, βyear4 = 0.22, p

  17. TISSUE INHIBITOR OF METALLOPROTEINASE 1, MATRIX METALLOPROTEINASE 9, ALPHA-1 ANTITRYPSIN, METALLOTHIONEIN AND UROKINASE TYPE PLASMINOGEN ACTIVATOR RECEPTOR IN SKIN BIOPSIES FROM PATIENTS AFFECTED BY AUTOIMMUNE BLISTERING DISEASES

    Ana Maria Abreu Velez

    2013-07-01

    Full Text Available Introduction: Proteinases and proteinase inhibitors have been described to play a role in autoimmune skin blistering diseases. We studied skin lesional biopsies from patients affected by several autoimmune skin blistering diseases for proteinases and proteinase inhibitors. Methods: We utilized immunohistochemistry to evaluate biopsies for alpha-1-antitrypsin, human matrix metalloproteinase 9 (MMP9, human tissue inhibitor of metalloproteinases 1 (TIMP-1, metallothionein and urokinase type plasminogen activator receptor (uPAR. We tested 30 patients affected by endemic pemphigus, 30 controls from the endemic area, and 15 normal controls. We also tested 30 biopsies from patients with bullous pemphigoid (BP, 20 with pemphigus vulgaris (PV, 8 with pemphigus foliaceus, and 14 with dermatitis herpetiformis (DH. Results: Contrary to findings in the current literature, most autoimmune skin blistering disease biopsies were negative for uPAR and MMP9. Only some chronic patients with El Bagre-EPF were positive to MMP9 in the dermis, in proximity to telocytes. TIMP-1 and metallothionein were positive in half of the biopsies from BP patients at the basement membrane of the skin, within several skin appendices, in areas of dermal blood vessel inflammation and within dermal mesenchymal-epithelial cell junctions.

  18. Phorbol ester induces the biosynthesis of glycosylated and nonglycosylated plasminogen activator inhibitor 2 in high excess over urokinase-type plasminogen activator in human U-937 lymphoma cells

    Genton, C.; Kruithof, E.K.; Schleuning, W.D.

    1987-03-01

    The tumor-promoting phorbol ester PMA induces changes in the histiocytic human lymphoma cell line U-937 akin to cellular differentiation and concomitantly stimulates the biosynthesis of plasminogen activator inhibitor 2 (PAI 2) and of urokinase-type plasminogen activator (u-PA). PAI 2 is found in a nonglycosylated intracellular and a glycosylated secreted form. The former appears to be identical to PAI 2 previously purified from placental extracts and large-scale U-937 cell cultures. The sixfold increase of PAI 2 antigen measured 24 h after PMA treatment in cell extracts and conditioned media is accompanied by an equal increase of active PAI 2 mRNA, whereas the 6 to 13-fold increase of u-PA antigen in the same samples is associated with only a 1.5-fold mRNA increase. The increase of PAI 2, but not of u-PA, biosynthesis requires transcription. A 50-fold molar excess of PAI 2 over u-PA is found in both extracts and conditioned media of PMA-treated cells. PAI 2 represents at least 0.3% of total de novo synthesized protein 24 h after induction with PMA. Thus, PAI 2, but not u-PA, is an abundant product of this precursor analogue of the mononuclear phagocyte lineage, and might represent a new marker for monocyte/macrophage differentiation.

  19. LOW DOSE MAGNESIUM SULPHATE REGIME FOR ECLAMPSIA

    Bangal V

    2009-09-01

    Full Text Available Pre- eclampsia is one of the commonest medical complications seen during pregnancy. It contributes significantly to maternal and perinatal morbidity and mortality. Dr.J.A.Pritchard in 1955, introduced magnesium sulphate for control of convulsions in eclampsia and is used worldwide. Considering the low body mass index of indian women, a low dose magnesium sulphate regime has been introduced by some authors. Present study was carried out at tertiary care centre in rural area. Fifty cases of eclampsia were randomly selected to find out the efficacy of low dose magnesium sulphate regime to control eclamptic convulsions. Maternal and perinatal outcome and magnesium toxicity were analyzed. It was observed that 86% cases responded to initial intravenous dose of 4 grams of 20% magnesium sulphate . Eight percent cases, who got recurrence of convulsion, were controlled by additional 2 grams of 20% magnesium sulphate. Six percent cases required shifting to standard Pritchard regime, as they did not respond to low dose magnesium sulphate regime. The average total dose of magnesium sulphate required for control of convulsions was 20 grams ie. 54.4% less than that of standard Pritchard regime. The maternal and perinatal morbidity and mortality in the present study werecomparable to those of standard Pritchard regime. The study did not find a single case of magnesium related toxicity with low dose magnesium sulphate regime. Low dose magnesium sulphate regime was found to be safe and effective in eclampsia.

  20. Explaining the Transition Between Exchange Rate Regimes

    Masson, Paul; RUGE-MURCIA, Francisco J.

    2003-01-01

    This paper studies the transition between exchange rate regimes using a Markov chain model with time-varying transition probabilities. The probabilities are parameterized as nonlinear functions of variables suggested by the currency crisis and optimal currency area literature. Results using annual data indicate that inflation, and to a lesser extent, output growth and trade openness help explain the exchange rate regime transition dynamics.

  1. LEGAL MATRIMONIAL REGIME IN B&H

    Boris Krešić

    2011-12-01

    Full Text Available Matrimonial regime between spouses or between extramarital partners, and between parents and children is regulated by the Family Law Act of Bosnia and Herzegovina Federation, hereinafter FLA B&HF (SG FBiH 35/05, 41/05, Family Law Act of the Republic of Srpska, hereinafter FLA RS (SG RS”54/02, 41/08 and the Family Law Act of Brčko District, hereinafter FLA BD (SG RS, 66/07. Legal rules used for the regulation of the matrimonial regime between spouses, as well as between spouses and third parties make matrimonial regime (Ponjavić, 2005, p. 361. Matrimonial regime between spouses in family legislation in Bosnia and Herzegovina (B&H is regulated in two following ways: as legal matrimonial regime and as contract matrimonial regime. Legal regime is the one which applies on spouses if not arranged otherwise prior to contracting marriage or during marriage. In this paper the author indicates the differences between the legal matrimonial regimes of the two entities as well as those between the entities and Brčko District of Bosnia and Herzegovina.

  2. Strengthening the nuclear non-proliferation regime

    Although the nuclear non-proliferation regime has enjoyed considerable success, today the regime has never been under greater threat. Three states have challenged the objectives of the NPT, and there is a technology challenge - the spread of centrifuge enrichment technology and know-how. A major issue confronting the international community is, how to deal with a determined proliferator? Despite this gloomy scenario, however, the non-proliferation regime has considerable strengths - many of which can be developed further. The regime comprises complex interacting and mutually reinforcing elements. At its centre is the NPT - with IAEA safeguards as the Treaty's verification mechanism. Important complementary elements include: restraint in the supply and the acquisition of sensitive technologies; multilateral regimes such as the CTBT and proposed FMCT; various regional and bilateral regimes; the range of security and arms control arrangements outside the nuclear area (including other WMD regimes); and the development of proliferation-resistant technologies. Especially important are political incentives and sanctions in support of non-proliferation objectives. This paper outlines some of the key issues facing the non-proliferation regime

  3. Synergies between nonproliferation regimes: A pragmatic approach

    Full text: With the recent progress in establishing international nonproliferation regimes, the question of synergies between different verification and monitoring regimes is becoming more acute. Three multilateral and universal nonproliferation organisations covering safeguards on civil nuclear materials, nuclear testing, and chemical weapons are up and running. A regime on biological weapons is under negotiation. Several regional organisations concerned with monitoring nonproliferation commitments in the nuclear field are in place; others are being established. Past discussions on synergies between these regimes have suffered from being too far-reaching. These discussions often have not reflected adequately the political difficulties of cooperation between regimes with different membership, scope and institutional set-up. This paper takes a pragmatic look at exploiting synergies and identifies some potential and real overlaps in the work between different verification regimes. It argues for a bottom-up approach and identifies building blocks for collaboration between verification regimes. By realising such, more limited potential for cooperation, the ground could be prepared for exploiting other synergies between these regimes. (author)

  4. Capacitance densitometer for flow regime identification

    Shipp, Jr., Roy L.

    1978-01-01

    This invention relates to a capacitance densitometer for determining the flow regime of a two-phase flow system. A two-element capacitance densitometer is used in conjunction with a conventional single-beam gamma densitometer to unambiguously identify the prevailing flow regime and the average density of a flowing fluid.

  5. The Association between HbA1c, Fasting Glucose, 1-Hour Glucose and 2-Hour Glucose during an Oral Glucose Tolerance Test and Cardiovascular Disease in Individuals with Elevated Risk for Diabetes

    Lind, Marcus; Tuomilehto, Jaakko; Uusitupa, Matti; Nerman, Olle; Eriksson, Johan; Ilanne-Parikka, Pirjo; Keinänen-Kiukaanniemi, Sirkka; Peltonen, Markku; Pivodic, Aldina; Lindström, Jaana

    2014-01-01

    Objective To determine the association between HbA1c, fasting plasma glucose (FPG), 1-hour (1 hPG) and 2-hour (2 hPG) glucose after an oral glucose tolerance test (OGTT) and cardiovascular disease in individuals with elevated risk for diabetes. Design We studied the relationship between baseline, updated mean and updated (last) value of HbA1c, FPG, 1 hPG and 2 hPG after an oral 75 g glucose tolerance test (OGTT) and acute CVD events in 504 individuals with impaired glucose tolerance (IGT) at baseline enrolled in the Finnish Diabetes Prevention Study. Setting Follow-up of clinical trial. Participants 504 individuals with IGT were followed with yearly evaluations with OGTT, FPG and HbA1c. Main Outcome Measure Relative risk of CVD. Results Over a median follow-up of 9.0 years 34 (6.7%) participants had a CVD event, which increased to 52 (10.3%) over a median follow-up of 13.0 years when including events that occurred among participants following a diagnosis of diabetes. Updated mean HbA1c, 1 hPG and 2 hPG, HR per 1 unit SD of 1.57 (95% CI 1.16 to 2.11), p = 0.0032, 1.51 (1.03 to 2.23), p = 0.036 and 1.60 (1.10 to 2.34), p = 0.014, respectively, but not FPG (p = 0.11), were related to CVD. In analyses of the last value prior to the CVD event the same three glycaemic measurements were associated with the CVD events, with HRs per 1 unit SD of 1.45 (1.06 to 1.98), p = 0.020, 1.55 (1.04 to 2.29), p = 0.030 and 2.19 (1.51 to 3.18), pdiabetes onset updated 2 hPG (p = 0.003) but not updated mean HbA1c (p = 0.08) was related to CVD. Conclusions and Relevance Current 2 hPG level in people with IGT is associated with increased risk of CVD. This supports its use in screening for prediabetes and monitoring glycaemic levels of people with prediabetes. PMID:25285769

  6. Feasibility of a dual regime gyrotron

    The design concept of a 42/84 GHz, 500 kW, CW, dual-regime gyrotron for ECRH of plasma in an experimental Tokamak will be presented in this paper. Operation at 42 GHz is fundamental where as that in 84 GHz will be second harmonic so that a similar guidance system will be retained for dual regime operation. In this paper, the mode competition and mode selection procedures are presented for such a dual regime operation. Cold cavity design and self-consistent calculations will be carried out for power and efficiencies. (author)

  7. Framing of regimes and transition strategies

    Jensen, Jens Stissing

    2012-01-01

    This article suggests that transition strategies are always formulated in the context of specific representations of the regime and the challenges it faces. It is argued that the framing of a regime affects the envisioning of transition strategies. An analysis of the current development agenda for...... the housing construction sector in Denmark reveals the relevance and impacts of different regime framings. It is proposed that the ability to cope with framing issues as situated and political processes is at the core of the governance of transitions....

  8. Earth Regime Network Evolution Study (ERNESt)

    Menrad, Bob

    2016-01-01

    Speaker and Presenter at the Lincoln Laboratory Communications Workshop on April 5, 2016 at the Massachusetts Institute of Technology Lincoln Laboratory in Lexington, MA. A visual presentation titled Earth Regimes Network Evolution Study (ERNESt).

  9. The nuclear marketplace and the nonproliferation regime

    The development and acceptance of the international nonproliferation regime has been one of the most remarkable achievements of the postwar era. The nonproliferation regime has developed incrementally over time, often in spite of the inability of the two superpowers to come to grips with their own arms race. States otherwise zealous to protect their sovereignty have accepted international constraints on that same sovereignty where nuclear power is concerned. The challenge to the nonproliferation regime, then, is both serious and multifaceted. There is no single or overriding source of that regime's problems, nor is there a single solution. Rolling back the NNPA and ''letting the US nuclear industry compete'' make for a good slogan but will not change the seriousness of the nonproliferation challenge

  10. The Two Regimes of Postwar Shipping

    Iversen, Martin Jes; Tenold, Stig

    2014-01-01

    the bargaining that accompanied the shift from the national regime to the competitive regime. Specifically, we show that the new regime primarily accommodated the interests of private actors such as shipping companies, rather than the interests of the authorities and the trade unions.......The aim of this article is to illustrate the most important changes in the regulatory framework of the shipping sector from the 1960s to 2010, and to analyse the basis for, and effects of, these changes. In order to explain how the transformation has occurred, we use two traditional maritime...... nations—Denmark and Norway—as case studies. First, we introduce the two regimes of Danish and Norwegian shipping: ‘the national regime’ from the early 1960s to the mid-1970s; and ‘the competitive regime’, which was fully established by the middle of the 1990s and still persists. Then, we briefly sketch...

  11. FLOW REGIMES BELOW AERATORS FOR DISCHARGE TUNNELS

    MA Fei; WU Jian-hua

    2012-01-01

    The flow regimes below an aerator influence directly the air entrainment and the cavitation damage control.Based on the theoretical considerations,the experiments of the aerator for a discharge tunnel were conducted,and the relationships between the flow regime and hydraulic and geometric parameters were investigated.The results showed that,there are two kinds of threshold values for the flow regime conversions.One is Fr1-2 standing for the conversion from the fully filled cavity to the partially filled cavity,and the other is Fr2-3 which shows the change from the partially filled cavity to the net air cavity.Two empirical expressions were obtained for the conversions of the flow regimes,which can be used in the designs of the aerators.

  12. What is the economy of Ancien Regime?

    Jean-Yves Grenier

    2012-01-01

    The aim of this article is to describe the main features of the Ancien Regime economy and develop a “political economy” of European societies, particularly the French, during the central period of modernity (XVII-XVIII centuries

  13. The international climate regime: towards consolidation collapse

    This article deals with the different modalities that exist to manage a problem of collective action in the field of climate negotiation. It uses two concepts of the International Political Economy (IPE): the concept of International Regime (IR) and the concept of Hegemony and / or Leadership. The course the international negotiation has taken between 1992 (Rio Convention) and march 2001 (the US rejection of the Kyoto Protocol of 1997) leads us, first, to question the conditions of existence as well as the viability of a non-hegemonic International Regime (Part One). Then, we discuss the perspectives for the 'post - Kyoto' era. After having examined the preferences of the three most active actors in the negotiation (USA, Europe, G77 + China) combined with the leadership capacities they possess, we identify three scenarios for the future: i) anarchy, ii) an international regime under the American hegemony, iii) an international regime under the European leadership (Part Two). (author)

  14. Inflation in the warm and cold regimes

    Berera, Arjun

    2006-01-01

    It is now understood that inflation dynamics comes in two forms, isentropic or cold inflation and nonisentropic or warm inflation. In the former, inflation occurs without radiation production, whereas in the latter both radiation production and inflation occur concurrently. Recent, detailed, quantum field theory calculations have shown that many generic inflation models, including hybrid inflation, which were believed only to have cold inflation regimes, in fact have regimes of both warm and ...

  15. Portfolio Selection with Jumps under Regime Switching

    Lin Zhao

    2010-01-01

    Full Text Available We investigate a continuous-time version of the mean-variance portfolio selection model with jumps under regime switching. The portfolio selection is proposed and analyzed for a market consisting of one bank account and multiple stocks. The random regime switching is assumed to be independent of the underlying Brownian motion and jump processes. A Markov chain modulated diffusion formulation is employed to model the problem.

  16. Molecular motors in conservative and dissipative regimes

    Perez-Carrasco, R.; Sancho, J. M.

    2011-10-01

    We present a theoretical study of a rotatory molecular motor under a conservative torque regime. We show that conservative and dissipative regimes present a different observable phenomenology. Our approach starts with a preliminary deterministic calculation of the motor cycle, which is complemented with stochastic simulations of a Langevin equation under a flashing ratchet potential. Finally, by using parameter values obtained from independent experimental information, our theoretical predictions are compared with experimental data of the F1-ATPase motor of the Bacillus PS3.

  17. Patent regimes and the commodification of knowledge

    Coriat, Benjamin; Weinstein, Olivier

    2011-01-01

    This paper analyses the evolution of the intellectual property regime (IPR), and more precisely the patent regime, in the USA since the 19th century. To do so, we consider intellectual property laws within the context of wider changes in capitalism, focusing on two main historical phases: firstly, the period covering the formation and development of 'corporate capitalism' dominated by large corporations and then the new phase, which opened up in the 1980s, marked by the rise to power of finan...

  18. Regime shifts and inflation uncertainty in Peru

    Paúl Castillo; Alberto Humala; Vicente Tuesta

    2012-01-01

    The link between inflation and inflation uncertainty is evaluated using Peruvian data, in a context of changing monetary policies because of regime shifts. A Markov regime-switching heteroskedasticity model that includes unobserved components is used. The model shows how periods of high (low) inflation accompany periods of high (low) short- and long-run uncertainty in inflation. The results of the model also illustrate how, during the recent period of price stability in Peru, both permanent a...

  19. Improving the taxation regime for electric power

    In Norway, the present taxation regime for electric power is very complex. The power companies are currently charged with ordinary tax on profits, tax on economic rent, tax on natural resources and land tax. In addition there are the rules about licence fees, yield of power due to concession conditions, and reversion. The Norwegian Electricity Industry Association (EBL), assisted by a firm of lawyers, has proposed an improvement over the current taxation regime

  20. Developmental Regimes in Africa synthesis report

    Booth, D.; Dietz, A.J.; Golooba-Mutebi, F.; Fuady, A.H.; Henley, D; Kelsall, T.; Leliveld, A.H.M.; Donge, van, J.K.

    2015-01-01

    Many African countries have experienced sustained economic growth, but few have achieved the type of structural change, driven by rising productivity, that has transformed mass living standards in parts of Asia. In the Developmental Regimes in Africa Synthesis Report, editor David Booth examines how DRA research has shed new light on how developmental regimes might emerge and be sustained in Africa in the 21st century. He outlines a concept with defining features at three levels: policy conte...

  1. Forest damage and snow avalanche flow regime

    T. Feistl; Bebi, P.; M. Christen; Margreth, S.; Diefenbach, L.; P. Bartelt

    2015-01-01

    Snow avalanches break, uproot and overturn trees causing damage to forests. The extent of forest damage provides useful information on avalanche frequency and intensity. However, impact forces depend on avalanche flow regime. In this paper, we define avalanche loading cases representing four different avalanche flow regimes: powder, intermittent, dry and wet. Using a numerical model that simulates both powder and wet snow avalanches, we study documented events with forest ...

  2. Regime Diagrams for K-Theory Dispersion

    Smith, Ronald B.

    2011-06-01

    In atmospheric dispersion, the "non-Gaussian" effects of gravitational settling, the vertical gradient in diffusivity and the surface deposition do not enter uniformly but rather break up parameter space into several discrete regimes. Here, we describe regime diagrams that are constructed for K-theory dispersion of effluent from a surface line source in unsheared inhomogeneous turbulence, using a previously derived Fourier-Hankel method. This K-theory formulation differs from the traditional one by keeping a non-zero diffusivity at the ground. This change allows for turbulent exchange between the canopy and the atmosphere and allows new natural length scales to emerge. The axes on the regime diagrams are non-dimensional distance defined as the ratio of downwind distance to the characteristic length scale for each effect. For each value of the ratio of settling speed to the K gradient, two to four regimes are found. Concentration formulae are given for each regime. The regime diagrams allow real dispersion problems to be categorized and the validity of end-state concentration formulae to be judged.

  3. The receptor for urokinase-plasminogen activator (uPAR) controls plasticity of cancer cell movement in mesenchymal and amoeboid migration style.

    Margheri, Francesca; Luciani, Cristina; Taddei, Maria Letizia; Giannoni, Elisa; Laurenzana, Anna; Biagioni, Alessio; Chillà, Anastasia; Chiarugi, Paola; Fibbi, Gabriella; Del Rosso, Mario

    2014-03-30

    The receptor for the urokinase plasminogen activator (uPAR) is up-regulated in malignant tumors. Historically the function of uPAR in cancer cell invasion is strictly related to its property to promote uPA-dependent proteolysis of extracellular matrix and to open a path to malignant cells. These features are typical of mesenchymal motility. Here we show that the full-length form of uPAR is required when prostate and melanoma cancer cells convert their migration style from the "path generating" mesenchymal to the "path finding" amoeboid one, thus conferring a plasticity to tumor cell invasiveness across three-dimensional matrices. Indeed, in response to a protease inhibitors-rich milieu, prostate and melanoma cells activated an amoeboid invasion program connoted by retraction of cell protrusions, RhoA-mediated rounding of the cell body, formation of a cortical ring of actin and a reduction of Rac-1 activation. While the mesenchymal movement was reduced upon silencing of uPAR expression, the amoeboid one was almost completely abolished, in parallel with a deregulation of small Rho-GTPases activity. In melanoma and prostate cancer cells we have shown uPAR colocalization with β1/β3 integrins and actin cytoskeleton, as well integrins-actin co-localization under both mesenchymal and amoeboid conditions. Such co-localizations were lost upon treatment of cells with a peptide that inhibits uPAR-integrin interactions. Similarly to uPAR silencing, the peptide reduced mesenchymal invasion and almost abolished the amoeboid one. These results indicate that full-length uPAR bridges the mesenchymal and amoeboid style of movement by an inward-oriented activity based on its property to promote integrin-actin interactions and the following cytoskeleton assembly. PMID:24681666

  4. Concentrations of plasminogen activator inhibitor-1 (PAI-1 and urokinase plasminogen activator (uPA in induced sputum of asthma patients after allergen challenge

    Krzysztof Kowal,

    2010-04-01

    Full Text Available Urokinase plasminogen activator (uPA and its inhibitor (PAI-1 are involved in tiisue remodeling and repairprocesses associated with acute and chronic inflammation. The aim of the study was to evaluate the effect of allergen challengeon concentration of uPA and PAI-1 in induced sputum of house dust mite allergic asthmatics (HDM-AAs. ThirtyHDM-AAs and ten healthy persons (HCswere recruited for the study. In 24 HDM-AAs bronchial challenge with Dermatophagoidespteronyssinus (Dp and in 6 HDM-AAs sham challenege with saline were performed. In HDM-AAs sputumwas induced 24 hours before (T0 and 24 hours (T24 after the challenge. Concentration of uPA and PAI-1 in induced sputumwere determined using immunoenzymatic assays. At T0 in HDM-AAs mean sputum uPA (151±96 pg/ml and PAI-1(4341±1262 pg/ml concentrations were higher than in HC (18.8±6.7 pg/ml; p=0.0002 and 596±180 pg/ml; p<0.0001; foruPA and PAI-1 respectively. After allergen challenge further increase in sputum uPA (187±144 pg/ml; p=0.03 and PAI-1(6252±2323 pg/ml; p<0.0001 concentrations were observed. Moreover, in Dp challenged, but not in saline challengedHDM-AAs the mean uPA/PAI-1 ratio decreased significantly at T24. No significant increase in the studied parameters werefound in sham challenged patients. In HDM-AAs allergen exposure leads to activation of the plasmin system in the airways.Greater increase of the PAI-1 concentration than uPA concentration after allergen challenge may promote airway remodelingand play an important role in the development of bronchial hyperreactivity.

  5. Concentrations of plasminogen activator inhibitor-1 (PAI-1 and urokinase plasminogen activator (uPA in induced sputum of asthma patients after allergen challenge.

    Marcin Moniuszko

    2011-04-01

    Full Text Available Urokinase plasminogen activator (uPA and its inhibitor (PAI-1 are involved in tiisue remodeling and repair processes associated with acute and chronic inflammation. The aim of the study was to evaluate the effect of allergen challenge on concentration of uPA and PAI-1 in induced sputum of house dust mite allergic asthmatics (HDM-AAs. Thirty HDM-AAs and ten healthy persons (HCswere recruited for the study. In 24 HDM-AAs bronchial challenge with Dermatophagoides pteronyssinus (Dp and in 6 HDM-AAs sham challenege with saline were performed. In HDM-AAs sputum was induced 24 hours before (T0 and 24 hours (T24 after the challenge. Concentration of uPA and PAI-1 in induced sputum were determined using immunoenzymatic assays. At T0 in HDM-AAs mean sputum uPA (151 Âą 96 pg/ml and PAI-1 (4341 Âą 1262 pg/ml concentrations were higher than in HC (18.8 Âą 6.7 pg/ml; p=0.0002 and 596 Âą 180 pg/ml; p<0.0001; for uPA and PAI-1 respectively. After allergen challenge further increase in sputum uPA (187 Âą 144 pg/ml; p=0.03 and PAI-1 (6252 Âą 2323 pg/ml; p<0.0001 concentrations were observed. Moreover, in Dp challenged, but not in saline challenged HDM-AAs the mean uPA/PAI-1 ratio decreased significantly at T24. No significant increase in the studied parameters were found in sham challenged patients. In HDM-AAs allergen exposure leads to activation of the plasmin system in the airways. Greater increase of the PAI-1 concentration than uPA concentration after allergen challenge may promote airway remodeling and play an important role in the development of bronchial hyperreactivity.

  6. Soluble Urokinase Receptor Levels Are Correlated with Focal Segmental Glomerulosclerosis Lesions in IgA Nephropathy: A Cohort Study from China.

    Shui-Ming Guo

    Full Text Available Soluble urokinase receptor (suPAR may be involved in the pathological mechanisms of focal segmental glomerulosclerosis (FSGS changes. However, it remains unclear whether suPAR is correlated with the FSGS-like lesions in IgA nephropathy (IgAN.We measured the plasma suPAR levels in 138 patients with IgAN, and then their clinical and pathological relationships were analyzed.We found that the plasma suPAR levels were significantly correlated with age and renal function by both univariate and multivariate analysis in our IgAN patient cohort. Female had higher plasma suPAR levels and no significant correlation was observed between plasma suPAR levels and 24-h urine protein and highly sensitive C-reaction protein with multivariate analysis. In our cohort, sixty of these IgAN patients could be diagnosed with a type of FSGS lesions. The plasma suPAR levels were higher in the IgAN patients with FSGS lesions than in the IgAN patients without FSGS lesions by univariate (P < 0.0001 and multivariate (P < 0.001 analysis adjusting for other predictor variables, which might be helpful to differentiate the pathological changes with and without FSGS lesions. And the optimal cutoff value was 1806 pg/ml in this study. The plasma suPAR concentrations were also associated with the degree of tubular atrophy/interstitial fibrosis in both univariate and multivariate analysis. In multivariate analysis, the plasma suPAR levels were correlated with the percentage of crescents, not global sclerosis and arterial lesions.Our study suggested that the plasma suPAR levels were associated with age, gender, renal function, the degree of tubular atrophy/interstitial fibrosis and the percentage of crescent formation. The plasma suPAR might be a potential predictor for the presence of FSGS pathological lesions in Chinese patients with IgAN.

  7. Staurosporine induces ganglion cell differentiation in part by stimulating urokinase-type plasminogen activator expression and activation in the developing chick retina

    Highlights: ► Staurosporine mediates stimulation of RGC differentiation in vitro cultured retinal neuroblasts. ► Staurosporine mediates uPA activation during RGC differentiation in vitro. ► Inhibition of uPA blocks the staurosporine mediated RGC differentiation both in vitro and in ovo. ► Thus, uPA may play a role in the staurosporine-mediated stimulation of RGC differentiation. -- Abstract: Here, we investigated whether staurosporine-mediated urokinase-type plasminogen activator (uPA) activation is involved in retinal ganglion cell (RGC) differentiation. Retinal cells were isolated from developing chick retinas at embryonic day 6 (E6). Relatively few control cells grown in serum-free medium started to form processes by 12 h. In contrast, staurosporine-treated cells had processes within 3 h, and processes were evident at 8 h. Immunofluorescence staining showed that Tuj-1-positive cells with shorter neurites could be detected in control cultures at 18 h, whereas numerous Tuj-1 positive ganglion cells with longer neuritic extensions were seen in staurosporine-treated cultures. BrdU-positive proliferating cells were more numerous in control cultures than in staurosporine-treated cultures, and the BrdU staining was not detected in post-mitotic Tuj-1 positive ganglion cells. Western blotting of cell lysates showed that staurosporine induced high levels of the active form of uPA. The staurosporine-induced uPA signal was localized predominantly in the soma, neurites and axons of Tuj-1-positive ganglion cells. Amiloride, an inhibitor of uPA, markedly reduced staurosporine-induced Tuj-1 staining, neurite length, neurite number, and uPA staining versus controls. In developing retinas in ovo, amiloride administration remarkably reduced the staurosporine-induced uPA staining and RGC differentiation. Taken together, our in vitro and in vivo data collectively indicate that uPA plays a role in the staurosporine-mediated stimulation of RGC differentiation.

  8. Elevated urinary levels of urokinase-type plasminogen activator receptor (uPAR) in pancreatic ductal adenocarcinoma identify a clinically high-risk group

    The urokinase plasminogen activator receptor is highly expressed and its gene is amplified in about 50% of pancreatic ductal adenocarcinomas; this last feature is associated with worse prognosis. It is unknown whether the level of its soluble form (suPAR) in urine may be a diagnostic-prognostic marker in these patients. The urinary level of suPAR was measured in 146 patients, 94 pancreatic ductal adenocarcinoma and 52 chronic pancreatitis. Urine from 104 healthy subjects with similar age and gender distribution served as controls. suPAR levels were normalized with creatinine levels (suPAR/creatinine, ng/mg) to remove urine dilution effect. Urinary suPAR/creatinine values of pancreatic ductal adenocarcinoma patients were significantly higher (median 9.8; 25th-75th percentiles 5.3-20.7) than those of either healthy donors (median 0; 0-0.5) or chronic pancreatitis patients (median 2.7; 0.9-4.7). The distribution of values among cancer patients was widespread and asymmetric, 53% subjects having values beyond the 95th percentile of healthy donors. The values of suPAR/creatinine did not correlate with tumour stage, Ca19-9 or CEA levels. Higher values correlated with poor prognosis among non-resected patients at univariate analysis; multivariate Cox regression identified high urinary suPAR/creatinine as an independent predictor of poor survival among all cancer patients (odds ratio 2.10, p = 0.0023), together with tumour stage (stage III odds ratio 2.65, p = 0.0017; stage IV odds ratio 4.61, p < 0.0001) and female gender (odds ratio 1.85, p = 0.01). A high urinary suPAR/creatinine ratio represents a useful marker for the identification of a subset of patients with poorer outcome

  9. Elevated urinary levels of urokinase-type plasminogen activator receptor (uPAR in pancreatic ductal adenocarcinoma identify a clinically high-risk group

    Brocco Giorgio

    2011-10-01

    Full Text Available Abstract Background The urokinase plasminogen activator receptor is highly expressed and its gene is amplified in about 50% of pancreatic ductal adenocarcinomas; this last feature is associated with worse prognosis. It is unknown whether the level of its soluble form (suPAR in urine may be a diagnostic-prognostic marker in these patients. Methods The urinary level of suPAR was measured in 146 patients, 94 pancreatic ductal adenocarcinoma and 52 chronic pancreatitis. Urine from 104 healthy subjects with similar age and gender distribution served as controls. suPAR levels were normalized with creatinine levels (suPAR/creatinine, ng/mg to remove urine dilution effect. Results Urinary suPAR/creatinine values of pancreatic ductal adenocarcinoma patients were significantly higher (median 9.8; 25th-75th percentiles 5.3-20.7 than those of either healthy donors (median 0; 0-0.5 or chronic pancreatitis patients (median 2.7; 0.9-4.7. The distribution of values among cancer patients was widespread and asymmetric, 53% subjects having values beyond the 95th percentile of healthy donors. The values of suPAR/creatinine did not correlate with tumour stage, Ca19-9 or CEA levels. Higher values correlated with poor prognosis among non-resected patients at univariate analysis; multivariate Cox regression identified high urinary suPAR/creatinine as an independent predictor of poor survival among all cancer patients (odds ratio 2.10, p = 0.0023, together with tumour stage (stage III odds ratio 2.65, p = 0.0017; stage IV odds ratio 4.61, p Conclusions A high urinary suPAR/creatinine ratio represents a useful marker for the identification of a subset of patients with poorer outcome.

  10. Effect of vacuum-assisted closure on the expression of urokinase-type plaminogen activator and urokinase-type plasminogen activator receptor in acute and chronic wounds healing%封闭负压引流技术对创面愈合过程纤溶酶原激活剂级联表达的影响

    李跃军; 曹大勇; 陈绍宗

    2006-01-01

    目的研究封闭负压引流技术对急慢性创面纤溶酶原激活剂(plasminogen activator,PA)级联中尿激酶型纤溶酶激活剂(urokinase-type plaminogen activator,uPA)和尿激酶型纤溶酶激活剂受体(urokinase-type plasminogen activator receptor,uPA)表达的影响.方法雄性小家猪5头,背部两侧形成急性创面,分为实验组和对照组,仅实验组动物接受封闭负压引流治疗.分别在治疗前和治疗后第1、3、6、9、12、18、25天于创缘切取标本,用兔抗人uPA和uPAR单克隆抗体按ABC程序进行免疫组织化学染色,并计算uPAA和uPAR的标记指数.人慢性创面6例,清创后进行持续封闭负压引流治疗,在治疗后第1、3、5、7天采集创面渗出液,并用ELISA法检测渗出液中uPA和uPAR的含量.结果猪急性创面在封闭负压引流治疗后uPA和uPAR均增加,在第3天达到高峰,然后急速下降,但实验组的基因表达量和染色强度均显著高于对照组.6例人慢性创面进行治疗前uPA和uPAR表达较多,随着治疗时间的延长而逐渐减少.结论封闭负压引流治疗既能上调急性创面伤口周围的表皮角质形成细胞的uPA和uPAR表达,使之迅速增殖迁移;又能通过抑制慢性创面uPA和uPAR表达,从而减少细胞外基质的降解.

  11. Fiscal Policy and Welfare under Different Exchange Rate Regimes

    Østrup, Finn

    regime in whichthe monetary authority optimises preferences which include an employment targetand an inflation target. As government spending affects the representativeindividual's utility, the choice of exchange rate regime has an impact on welfare.Keywords: exchange rate regimes; fiscal policy...

  12. Laser-Nucleus Interactions: The Quasiadiabatic Regime

    Pálffy, Adriana; Hoefer, Axel; Weidenmüller, Hans A

    2015-01-01

    The interaction between nuclei and a strong zeptosecond laser pulse with coherent MeV photons is investigated theoretically. We provide a first semi-quantitative study of the quasiadiabatic regime where the photon absorption rate is comparable to the nuclear equilibration rate. In that regime, multiple photon absorption leads to the formation of a compound nucleus in the so-far unexplored regime of excitation energies several hundred MeV above the yrast line. The temporal dynamics of the process is investigated by means of a set of master equations that account for dipole absorption, stimulated dipole emission, neutron decay and induced fission in a chain of nuclei. That set is solved numerically by means of state-of-the-art matrix exponential methods also used in nuclear fuel burnup and radioactivity transport calculations. Our quantitative estimates predict the excitation path and range of nuclei reached by neutron decay and provide relevant information for the layout of future experiments.

  13. Fermi's golden rule beyond the Zeno regime

    Debierre, Vincent; Goessens, Isabelle; Brainis, Edouard; Durt, Thomas

    2015-08-01

    We reconsider the problem of the spontaneous emission of light by an excited atomic state. We scrutinize the survival probability of this excited state for very short times, in the so-called Zeno regime, for which we show that the dynamics is dictated by a coherent, in-phase, response of the on-shell and off-shell vacuum modes. We also develop a perturbative approach in order to interpolate between different temporal regimes: the Zeno, golden rule (linear), and Wigner-Weisskopf (exponential) regimes. We compare results obtained with the E ̂.x ̂ and A ̂.p ̂ interaction Hamiltonians, using successively the dipole approximation and the exact coupling.

  14. Stochastic dynamical models for ecological regime shifts

    Møller, Jan Kloppenborg; Carstensen, Jacob; Madsen, Henrik; Andersen, Tom

    Ecosystems are influenced by a variety of known and unknown drivers. Unknown drivers should be modeled as noise and it is therefore important to analyze how noise influences the deterministic skeleton of system equations. The deterministic skeleton of stochastic dynamical models contains the...... physical and biological knowledge of the system, and nonlinearities introduced here can generate regime shifts or enhance the probability of regime shifts in the case of stochastic models, typically characterized by a threshold value for the known driver. A simple model for light competition between...... phytoplankton and benthic vegetation with feedback mechanisms is formulated, and it is demonstrated that bistability can occur for specific parameter settings. When stochastic input and stochastic propagation of the states are applied on the system regime shifts occur more frequently, and the threshold...

  15. Determination of the Hall Thruster Operating Regimes

    L. Dorf; V. Semenov; Y. Raitses; N.J. Fisch

    2002-04-09

    A quasi one-dimensional (1-D) steady-state model of the Hall thruster is presented. For the same discharge voltage two operating regimes are possible -- with and without the anode sheath. For given mass flow rate, magnetic field profile and discharge voltage a unique solution can be constructed, assuming that the thruster operates in one of the regimes. However, we show that for a given temperature profile the applied discharge voltage uniquely determines the operating regime: for discharge voltages greater than a certain value, the sheath disappears. That result is obtained over a wide range of incoming neutral velocities, channel lengths and widths, and cathode plane locations. It is also shown that a good correlation between the quasi 1-D model and experimental results can be achieved by selecting an appropriate electron mobility and temperature profile.

  16. Progress towards a global nuclear liability regime

    During its April 2014 meeting, the Steering Committee for Nuclear Energy held a policy debate on 'Progress towards a Global Nuclear Liability Regime'. The Steering Committee heard presentations from several experts on nuclear liability issues. To prepare the delegates to the Steering Committee for the policy debate, the NEA Secretariat prepared a background note on the status of the nuclear liability regimes, as well as on current issues and challenges in implementing the regimes. This article is based on the background note and is intended to provide basic information on the relevant international conventions and an overview of recent developments to enhance the understanding of the legal framework in which policy-makers and practitioners are engaging to respond to the call for broader adherence to the international liability instruments. (authors)

  17. Effect Observation of Small Dose Urokinase in Treatment of Thrombolytic Therapy of Acute Myocardial Infarction%小剂量尿激酶在急性心肌梗塞溶栓治疗中的疗效分析

    刘兴君

    2015-01-01

    目的:小剂量尿激酶在心肌梗死溶栓治疗中的疗效分析。方法选取2012年7月—2014年4月该院收治的急性心肌梗塞患者75例,并分为常规组(n=38)和小剂量组(n=37)。常规组给予150U尿激酶治疗,小剂量组给予50U尿激酶治疗,比较两组的溶栓再通率、急性期死亡率和溶栓不良反应。结果常规组溶栓总再通率为84.21%,显著高于小剂量组45.94%(c2=12.119,P=0.000);常规治疗组的急性期死亡率为2.63%,显著低于小剂量组33.33%(c2=4.730,P=0.030)。常规剂量组,溶栓治疗后不良发生率为18.42%,小剂量组则为18.91%,两组比较,差异无统计学意义(c2=0.003,P=0.956)。结论在急性心肌梗塞溶栓治疗中,需给予足量的尿激酶,小剂量的治疗效果不甚理想。%Objective To investigate effect of small dose urokinase in treatment of thrombolytic therapy of acute myocardial infarction.Methods 75 patients from 2012 July to 2014 April in our hospital were selected,and were divided into two groups.The routine group was given 150U urokinase,while small dose group was given 50U urokinase.Thrombolytic recanalization rate, acute mortality and adverse reaction rate of two groups were compared.Results Thrombolysis total recanalization rate of routine group was 84.21%,which was significantly higher than the small dose group(45.94%)(c2=12.119,P=0.000). Thrombolysis total recanalization rate of routine group was 2.63%,which was significantly lower than that of the small dose group(18.91%) (c2=4.730,P=0.030). The adverse reaction rate of routine group was 18.42%,while routine group was 18.91%,and there was no significant difference between two groups(c2=0.003,P=0.956).Conclusion In the thrombolytic therapy of acute myocardial infarction treatment, enough small dose urokinase should be given, and treatment effect of small dose of urokinase is not very ideal.

  18. Causas estruturais e consequências dos regimes internacionais: regimes como variáveis intervenientes

    Stephen D. Krasner

    2012-06-01

    Full Text Available Os regimes internacionais são definidos como princípios, normas, regras e procedimentos de tomada de decisões ao redor dos quais as expectativas dos atores convergem em uma dada área-tema. Como ponto de partida, os regimes são conceituados como variáveis intervenientes, estando entre fatores causais básicos e os resultados e comportamentos relacionados. Há três visões a respeito da importância dos regimes: as orientações estruturais convencionais desvalorizam os regimes como sendo, na melhor das hipóteses, ineficazes; as orientações grocianas vêem os regimes como componentes íntimos do sistema internacional; as perspectivas estruturalistas modificadas vêem os regimes como significativos somente em certas condições restritas. Para os argumentos grociano e estruturalista modificado - que concordam com a visão de que os regimes podem influenciar resultados e comportamentos - , o desenvolvimento de regimes é visto como uma função de cinco variáveis causais básicas: auto-interesse egoísta; poder político; normas e princípios difusos; usos e costumes; conhecimento.

  19. Supercurrent in the quantum Hall regime.

    Amet, F; Ke, C T; Borzenets, I V; Wang, J; Watanabe, K; Taniguchi, T; Deacon, R S; Yamamoto, M; Bomze, Y; Tarucha, S; Finkelstein, G

    2016-05-20

    A promising route for creating topological states and excitations is to combine superconductivity and the quantum Hall (QH) effect. Despite this potential, signatures of superconductivity in the QH regime remain scarce, and a superconducting current through a QH weak link has been challenging to observe. We demonstrate the existence of a distinct supercurrent mechanism in encapsulated graphene samples contacted by superconducting electrodes, in magnetic fields as high as 2 tesla. The observation of a supercurrent in the QH regime marks an important step in the quest for exotic topological excitations, such as Majorana fermions and parafermions, which may find applications in fault-tolerant quantum computing. PMID:27199424

  20. Supercurrent in the quantum Hall regime

    Amet, F.; Ke, C. T.; Borzenets, I. V.; Wang, J.; Watanabe, K.; Taniguchi, T.; Deacon, R. S.; Yamamoto, M.; Bomze, Y.; Tarucha, S.; Finkelstein, G.

    2016-05-01

    A promising route for creating topological states and excitations is to combine superconductivity and the quantum Hall (QH) effect. Despite this potential, signatures of superconductivity in the QH regime remain scarce, and a superconducting current through a QH weak link has been challenging to observe. We demonstrate the existence of a distinct supercurrent mechanism in encapsulated graphene samples contacted by superconducting electrodes, in magnetic fields as high as 2 tesla. The observation of a supercurrent in the QH regime marks an important step in the quest for exotic topological excitations, such as Majorana fermions and parafermions, which may find applications in fault-tolerant quantum computing.

  1. The oil tax regime of Azerbaijan

    Anderson, Gerard

    1998-07-01

    Azerbaijan has a long history in the oil business and a chance of a spectacular future. To understand why the oil tax regime evolved into its present form and how it is likely to develop, it is necessary to know something of the country's history and the commercial environment. Consequently the presentation begins by discussing these items. It then outlines the Production Sharing Agreement regime in Azerbaijan and then deals with the Kazakh and Georgian Tax Codes, as these are likely to be the basis of a new general tax law in Azerbaijan from 1999. The presentation includes comments on the New Draft Tax Code of 1998.

  2. WELFARE REGIMES IN LATIN AMERICA AND CARIBBEAN

    Melisa Campana-Alabarce

    2015-06-01

    Full Text Available This article provides a characterization of Latin American and Caribbean Welfare regimes in historiographical perspective. Firstly, it makes a review of the emergence conditions of Welfare States in Western Europe and its core features, with particular emphasis on its role as a method to regulate inequalities in industrial capitalism. Dialoguing with it, then stops in the specific configurations that welfare regimes have taken in Latin America during the course of the twentieth century. Finally, it provides a map of its contemporary features and the major challenges that the States of the region face in his capacity as right guarantors for the future.

  3. High temperature regime of corium concrete interactions

    The high temperature regime of corium concrete interaction is examined from the point of view of its sensitivity to the completeness of reaction of the gaseous concrete decomposition products with the metallic components of the melt. A program based on the DECOMP modeling approach has been utilized for this purpose. For a corium/concrete heat transfer that is consistent with the erosion rates observed in the BETA experiments the primary effect of the extent of reaction completeness is on the duration of the high temperature regime. This implies a potentially important effect on the aerosols stripped out during this portion of the interaction

  4. Primary Qualification of Matrimonial Regime Notion

    Nadia Cerasela ANITEI

    2012-01-01

    By adopting Law no. 287 of July 17, 2009 on the Civil Code republished by Law no. 71/2011 the new Civil Code is subject to the modern legislations tendencies to create a triple balance in terms of property relations between spouses by means of the matrimonial property regimes established:1. between spouses: through the appearance of matrimonial agreements, which have led to the adoption of more flexible legal rules which allow spouses a certain freedom to choose the regime of patrimonial rela...

  5. Light focusing in the Anderson Regime

    Leonetti, Marco; Mafi, Arash; Conti, Claudio

    2014-01-01

    Anderson localization is a regime in which diffusion is inhibited and waves (also electromagnetic waves) get localized. Here we exploit adaptive optics to achieve focusing in disordered optical fibers in the Anderson regime. By wavefront shaping and optimization, we observe the generation of a propagation invariant beam, where light is trapped transversally by disorder, and show that Anderson localizations can be also excited by extended speckled beams. We demonstrate that disordered fibers allow a more efficient focusing action with respect to standard fibers in a way independent of their length, because of the propagation invariant features and cooperative action of transverse localizations.

  6. Quaternary Faults and Stress Regime of Venezuela

    F.A. Audemard M.; A. Singer P.; J-P. Soulas

    2006-01-01

    Spatial configuration of Quaternary active tectonic features along the southern Caribbean plate boundary suggests that the region is subject to a compressive strike-slip (transpressional senso lato) regime, characterized by a NNW-SSE maximum horizontal stress (sH=s1) and/or an ENE-WSW minimum (s h=s3 or s2) horizontal stress. Stress inversion applied to fault-plane kinematic indicators measured essentially in Plio-Quaternary sedimentary rocks confirms this tectonic regime. Accordingly, this s...

  7. Economic performance and North Korean regime legitimacy

    Moore, Patrick J.

    2014-01-01

    This thesis examines the sources of legitimacy for the North Korean regime in an effort to explain what role, if any, economic performance has played in keeping the Kim family in power. This thesis provides a historical look at the development of the North Korean regime from the beginning under Kim Il-sung to the current generation of rule under Kim Jong-un. The core argument of the thesis is broken into two major time periods, with the economic downturn of the early 1990s serving as the divi...

  8. Development of quantitative RT-PCR assays for wild-type urokinase receptor (uPAR-wt) and its splice variant uPAR-del5

    The receptor for the serine protease urokinase-type plasminogen activator, uPAR (CD 87), plays an important role in tumor cell invasion and metastasis of solid malignant tumors. uPAR is a highly glycosylated, glycan lipid-anchored membrane protein, consisting of three homologous domains. Each individual domain is encoded by two exons: DI by exons 2+3, DII by exons 4+5, and DIII by exons 6+7. Beside the wild-type (wt) uPAR mRNA, two splice variants either lacking exon 5 (uPAR-del5) or both exons 4 and 5 (uPARdel4/ 5) have been described. Previously, we studied expression of the mRNA variant uPAR-del4/5 and uPAR mRNA encompassing exons 2, 3, and 4 (i.e. uPAR-wt plus uPAR-del5) applying real-time RT-PCR assays for quantification of the mRNA concentration. In the present paper, we established two additional specific, robust and highly sensitive RT-PCR assays, based on the LightCycler technology, to specifically quantify either uPAR-wt or its splice variant, uPARdel5. Expression of uPAR-wt and uPAR-del5 was analyzed in different human malignant cell lines (ovarian cancer cell lines OVMZ-6 and OVMZ-10; breast cancer cell lines MDA-MB 231, MDA-MB 231 BAG, MDA-MB 435, and aMCF-7; brain tumor cell line LN 18) as well as in a set of 174 breast cancer tissue samples. uPAR-del5 mRNA was found to be expressed very frequently at a rather low level (typically less than 1% of uPAR-wt mRNA). In tumor tissue from breast cancer patients, a statistically significant correlation between uPAR-del5 and uPAR-wt mRNA (r = 0.779; P < 0.001) was observed. There was no association between the expression level of either mRNA and clinical parameters such as nodal status, tumor size and grade. In estrogen receptor negative tumors, a significantly higher uPAR-del5 expression was found (P 0.023). The two developed quantitative RT-PCR assays described here may aid further analysis of the function and clinical relevance of uPAR-wt and one of its splice variants, uPAR-del5, in malignant tumors

  9. A combination of desmopressin and docetaxel inhibit cell proliferation and invasion mediated by urokinase-type plasminogen activator (uPA) in human prostate cancer cells

    Sasaki, Hiroshi; Klotz, Laurence H. [Division of Urology, Sunnybrook Health Sciences Center, Toronto, ON (Canada); Sugar, Linda M. [Department of Pathology, Sunnybrook Health Sciences Center, Toronto, ON (Canada); Kiss, Alexander [Department of Research Design and Biostatistics, Institute for Clinical Evaluative Sciences, Sunnybrook Health Sciences Center, Toronto, ON (Canada); Venkateswaran, Vasundara, E-mail: vasundara.venkateswaran@sunnybrook.ca [Division of Urology, Sunnybrook Health Sciences Center, Toronto, ON (Canada)

    2015-08-28

    Background: This study was designed to assess the effectiveness of a combination treatment using both desmopressin and docetaxel in prostate cancer treatment. Desmopressin is a well-known synthetic analogue of the antidiuretic hormone vasopressin. It has recently been demonstrated to inhibit tumor progression and metastasis in in vivo models. Docetaxel is widely used for the treatment of castration resistant prostate cancer (CRPC) patients. However, durable responses have been uncommon to date. In this study, we investigated the anti-tumor effect of desmopressin in combination with docetaxel in vitro and in vivo. Methods: Two prostate cancer cells (PC3, LNCaP) were treated with different concentrations of desmopressin alone, docetaxel alone, and a combination of desmopressin and docetaxel. Cell proliferation was determined by MTS assay. The anti-invasive and anti-migration potential of desmopressin and in combination with docetaxel were examined by wound healing assay, migration chamber assay, and matrigel invasion assay. Results: The combination of desmopressin and docetaxel resulted in a significant inhibition of PC3 and LNCaP cell proliferation (p < 0.01). Additionally, cell migration and invasion were also inhibited by the combination when compared to that of either treatment alone in PC3 cells (p < 0.01). The anti-tumor effect of this combination treatment was associated with down-regulation of both urokinase-type plasminogen activator (uPA) and matrix metalloproteinase (MMP-2 and MMP-9) in PC3 cells. Conclusions: We are the first to elucidate the anti-tumor and anti-metastatic potential of desmopressin in combination with docetaxel in a prostate cancer model via the uPA-MMP pathway. Our finding could potentially contribute to the therapeutic profile of desmopressin and enhance the efficacy of docetaxel based treatment for CRPC. - Highlights: • Desmopressin inhibits cell proliferation in prostate cancer cells. • The expression of cyclin A and CDK2

  10. Inhibition of urokinase plasminogen activator “uPA” activity alters ethanol consumption and conditioned place preference in mice

    Al Maamari E

    2014-09-01

    Full Text Available Elyazia Al Maamari,* Mouza Al Ameri, Shamma Al Mansouri, Amine Bahi*Department of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates*These authors contributed equally to this workAbstract: Urokinase plasminogen activator, uPA, is a serine protease implicated in addiction to drugs of abuse. Using its specific inhibitor, B428, we and others have characterized the role of uPA in the rewarding properties of psychostimulants, including cocaine and amphetamine, but none have examined the role of uPA in ethanol use disorders. Therefore, in the current study, we extended our observations to the role of uPA in ethanol consumption and ethanol-induced conditioned place preference. The general aim of the present series of experiments was to investigate the effects of the administration of the B428 on voluntary alcohol intake and ethanol conditioned reward. A two-bottle choice, unlimited-access paradigm was used to compare ethanol intake between vehicle- and 3, 10, and 30 mg/kg B428-administered mice. For this purpose, the mice were presented with an ethanol solution (2.5%–20% and water, at each concentration for 4 days, and their consumption was measured daily. Consumption of saccharin and quinine solutions was also measured. Systemic administration of B428 dose-dependently decreased ethanol intake and preference. Additionally, B428 mice did not differ from vehicle mice in their intake of graded solutions of tastants, suggesting that the uPA inhibition did not alter taste function. Also, ethanol metabolism was not affected following B428 injection. More importantly, 1.5 g/kg ethanol-induced conditioned place preference acquisition was blocked following B428 administration. Taken together, our results are the first to implicate uPA inhibition in the regulation of ethanol consumption and preference, and suggest that uPA may be considered as a possible therapeutic drug target for alcoholism and

  11. Direct interaction of the kringle domain of urokinase-type plasminogen activator (uPA) and integrin alpha v beta 3 induces signal transduction and enhances plasminogen activation.

    Tarui, Takehiko; Akakura, Nobuaki; Majumdar, Mousumi; Andronicos, Nicholas; Takagi, Junichi; Mazar, Andrew P; Bdeir, Khalil; Kuo, Alice; Yarovoi, Serge V; Cines, Douglas B; Takada, Yoshikazu

    2006-03-01

    It has been questioned whether there are receptors for urokinase-type plasminogen activator (uPA) that facilitate plasminogen activation other than the high affinity uPA receptor (uPAR/CD87) since studies of uPAR knockout mice did not support a major role of uPAR in plasminogen activation. uPA also promotes cell adhesion, chemotaxis, and proliferation besides plasminogen activation. These uPA-induced signaling events are not mediated by uPAR, but mediated by unidentified, lower-affinity receptors for the uPA kringle. We found that uPA binds specifically to integrin alpha v beta 3 on CHO cells depleted of uPAR. The binding of uPA to alpha v beta 3 required the uPA kringle domain. The isolated uPA kringle domain binds specifically to purified, recombinant soluble, and cell surface alpha v beta 3, and other integrins (alpha 4 beta 1 and alpha 9 beta 1), and induced migration of CHO cells in an alpha v beta 3-dependent manner. The binding of the uPA kringle to alpha v beta 3 and uPA kringle-induced alpha v beta 3-dependent cell migration were blocked by homologous plasminogen kringles 1-3 or 1-4 (angiostatin), a known integrin antagonist. We studied whether the binding of uPA to integrin alpha v beta 3 through the kringle domain plays a role in plasminogen activation. On CHO cell depleted of uPAR, uPA enhanced plasminogen activation in a kringle and alpha v beta 3-dependent manner. Endothelial cells bound to and migrated on uPA and uPA kringle in an alpha v beta 3-dependent manner. These results suggest that uPA binding to integrins through the kringle domain plays an important role in both plasminogen activation and uPA-induced intracellular signaling. The uPA kringle-integrin interaction may represent a novel therapeutic target for cancer, inflammation, and vascular remodeling. PMID:16525582

  12. Nicotine stimulates urokinase-type plasminogen activator receptor expression and cell invasiveness through mitogen-activated protein kinase and reactive oxygen species signaling in ECV304 endothelial cells

    Khoi, Pham Ngoc; Park, Jung Sun; Kim, Nam Ho; Jung, Young Do, E-mail: ydjung@chonnam.ac.kr

    2012-03-01

    Urokinase-type plasminogen activator receptor (uPAR) expression is elevated during inflammation, tissue remodeling and in many human cancers. This study investigated the effect of nicotine, a major alkaloid in tobacco, on uPAR expression and cell invasiveness in ECV304 endothelial cells. Nicotine stimulated uPAR expression in a dose-dependent manner and activated extracellular signal-regulated kinases-1/2 (Erk-1/2), c-Jun amino-terminal kinase (JNK) and p38 mitogen activated protein kinase (MAPK). Specific inhibitors of MEK-1 (PD98059) and JNK (SP600125) inhibited the nicotine-induced uPAR expression, while the p38 MAPK inhibitor SB203580 did not. Expression vectors encoding dominant negative MEK-1 (pMCL-K97M) and JNK (TAM67) also prevented nicotine-induced uPAR promoter activity. The intracellular hydrogen peroxide (H{sub 2}O{sub 2}) content was increased by nicotine treatment. The antioxidant N-acetylcysteine prevented nicotine-activated production of reactive oxygen species (ROS) and uPAR expression. Furthermore, exogenous H{sub 2}O{sub 2} increased uPAR mRNA expression. Deleted and site-directed mutagenesis demonstrated the involvement of the binding sites of transcription factor nuclear factor-kappaB (NF-κB) and activator protein (AP)-1 in the nicotine-induced uPAR expression. Studies with expression vectors encoding mutated NF-κB signaling molecules and AP-1 decoy confirmed that NF-κB and AP-1 were essential for the nicotine-stimulated uPAR expression. MAPK (Erk-1/2 and JNK) and ROS functioned as upstream signaling molecules in the activation of AP-1 and NF-κB, respectively. In addition, ECV304 endothelial cells treated with nicotine displayed markedly enhanced invasiveness, which was partially abrogated by uPAR neutralizing antibodies. The data indicate that nicotine induces uPAR expression via the MAPK/AP-1 and ROS/NF-κB signaling pathways and, in turn, stimulates invasiveness in human ECV304 endothelial cells. -- Highlights: ► Endothelial cells

  13. Nicotine stimulates urokinase-type plasminogen activator receptor expression and cell invasiveness through mitogen-activated protein kinase and reactive oxygen species signaling in ECV304 endothelial cells

    Urokinase-type plasminogen activator receptor (uPAR) expression is elevated during inflammation, tissue remodeling and in many human cancers. This study investigated the effect of nicotine, a major alkaloid in tobacco, on uPAR expression and cell invasiveness in ECV304 endothelial cells. Nicotine stimulated uPAR expression in a dose-dependent manner and activated extracellular signal-regulated kinases-1/2 (Erk-1/2), c-Jun amino-terminal kinase (JNK) and p38 mitogen activated protein kinase (MAPK). Specific inhibitors of MEK-1 (PD98059) and JNK (SP600125) inhibited the nicotine-induced uPAR expression, while the p38 MAPK inhibitor SB203580 did not. Expression vectors encoding dominant negative MEK-1 (pMCL-K97M) and JNK (TAM67) also prevented nicotine-induced uPAR promoter activity. The intracellular hydrogen peroxide (H2O2) content was increased by nicotine treatment. The antioxidant N-acetylcysteine prevented nicotine-activated production of reactive oxygen species (ROS) and uPAR expression. Furthermore, exogenous H2O2 increased uPAR mRNA expression. Deleted and site-directed mutagenesis demonstrated the involvement of the binding sites of transcription factor nuclear factor-kappaB (NF-κB) and activator protein (AP)-1 in the nicotine-induced uPAR expression. Studies with expression vectors encoding mutated NF-κB signaling molecules and AP-1 decoy confirmed that NF-κB and AP-1 were essential for the nicotine-stimulated uPAR expression. MAPK (Erk-1/2 and JNK) and ROS functioned as upstream signaling molecules in the activation of AP-1 and NF-κB, respectively. In addition, ECV304 endothelial cells treated with nicotine displayed markedly enhanced invasiveness, which was partially abrogated by uPAR neutralizing antibodies. The data indicate that nicotine induces uPAR expression via the MAPK/AP-1 and ROS/NF-κB signaling pathways and, in turn, stimulates invasiveness in human ECV304 endothelial cells. -- Highlights: ► Endothelial cells treated with nicotine

  14. Urokinase plasminogen activator inhibits HIV virion release from macrophage-differentiated chronically infected cells via activation of RhoA and PKCε.

    Francesca Graziano

    Full Text Available BACKGROUND: HIV replication in mononuclear phagocytes is a multi-step process regulated by viral and cellular proteins with the peculiar feature of virion budding and accumulation in intra-cytoplasmic vesicles. Interaction of urokinase-type plasminogen activator (uPA with its cell surface receptor (uPAR has been shown to favor virion accumulation in such sub-cellular compartment in primary monocyte-derived macrophages and chronically infected promonocytic U1 cells differentiated into macrophage-like cells by stimulation with phorbol myristate acetate (PMA. By adopting this latter model system, we have here investigated which intracellular signaling pathways were triggered by uPA/uPAR interaction leading the redirection of virion accumulation in intra-cytoplasmic vesicles. RESULTS: uPA induced activation of RhoA, PKCδ and PKCε in PMA-differentiated U1 cells. In the same conditions, RhoA, PKCδ and PKCε modulated uPA-induced cell adhesion and polarization, whereas only RhoA and PKCε were also responsible for the redirection of virions in intracellular vesicles. Distribution of G and F actin revealed that uPA reorganized the cytoskeleton in both adherent and polarized cells. The role of G and F actin isoforms was unveiled by the use of cytochalasin D, a cell-permeable fungal toxin that prevents F actin polymerization. Receptor-independent cytoskeleton remodeling by Cytochalasin D resulted in cell adhesion, polarization and intracellular accumulation of HIV virions similar to the effects gained with uPA. CONCLUSIONS: These findings illustrate the potential contribution of the uPA/uPAR system in the generation and/or maintenance of intra-cytoplasmic vesicles that actively accumulate virions, thus sustaining the presence of HIV reservoirs of macrophage origin. In addition, our observations also provide evidences that pathways controlling cytoskeleton remodeling and activation of PKCε bear relevance for the design of new antiviral strategies aimed

  15. Staurosporine induces ganglion cell differentiation in part by stimulating urokinase-type plasminogen activator expression and activation in the developing chick retina

    Kim, Yeoun-Hee [Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu 702-701 (Korea, Republic of); Chang, Yongmin [Department of Molecular Medicine, Kyungpook National University College of Medicine, Kyungpook National University, 200 Dongduk-Ro Jung-Gu, Daegu 700-714 (Korea, Republic of); Jung, Jae-Chang, E-mail: jcjung@knu.ac.kr [Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu 702-701 (Korea, Republic of)

    2012-06-22

    Highlights: Black-Right-Pointing-Pointer Staurosporine mediates stimulation of RGC differentiation in vitro cultured retinal neuroblasts. Black-Right-Pointing-Pointer Staurosporine mediates uPA activation during RGC differentiation in vitro. Black-Right-Pointing-Pointer Inhibition of uPA blocks the staurosporine mediated RGC differentiation both in vitro and in ovo. Black-Right-Pointing-Pointer Thus, uPA may play a role in the staurosporine-mediated stimulation of RGC differentiation. -- Abstract: Here, we investigated whether staurosporine-mediated urokinase-type plasminogen activator (uPA) activation is involved in retinal ganglion cell (RGC) differentiation. Retinal cells were isolated from developing chick retinas at embryonic day 6 (E6). Relatively few control cells grown in serum-free medium started to form processes by 12 h. In contrast, staurosporine-treated cells had processes within 3 h, and processes were evident at 8 h. Immunofluorescence staining showed that Tuj-1-positive cells with shorter neurites could be detected in control cultures at 18 h, whereas numerous Tuj-1 positive ganglion cells with longer neuritic extensions were seen in staurosporine-treated cultures. BrdU-positive proliferating cells were more numerous in control cultures than in staurosporine-treated cultures, and the BrdU staining was not detected in post-mitotic Tuj-1 positive ganglion cells. Western blotting of cell lysates showed that staurosporine induced high levels of the active form of uPA. The staurosporine-induced uPA signal was localized predominantly in the soma, neurites and axons of Tuj-1-positive ganglion cells. Amiloride, an inhibitor of uPA, markedly reduced staurosporine-induced Tuj-1 staining, neurite length, neurite number, and uPA staining versus controls. In developing retinas in ovo, amiloride administration remarkably reduced the staurosporine-induced uPA staining and RGC differentiation. Taken together, our in vitro and in vivo data collectively indicate that

  16. Involvement of nitric oxide synthase in matrix metalloproteinase-9- and/or urokinase plasminogen activator receptor-mediated glioma cell migration

    Src tyrosine kinase activates inducible nitric oxide synthase (iNOS) and, in turn, nitric oxide production as a means to transduce cell migration. Src tyrosine kinase plays a key proximal role to control α9β1 signaling. Our recent studies have clearly demonstrated the role of α9β1 integrin in matrix metalloproteinase-9 (MMP-9) and/or urokinase plasminogen activator receptor (uPAR)-mediated glioma cell migration. In the present study, we evaluated the involvement of α9β1 integrin-iNOS pathway in MMP-9- and/or uPAR-mediated glioma cell migration. MMP-9 and uPAR shRNAs and overexpressing plasmids were used to downregulate and upregulate these molecules, respectively in U251 glioma cells and 5310 glioma xenograft cells. The effect of treatments on migration and invasion potential of these glioma cells were assessed by spheroid migration, wound healing, and Matrigel invasion assays. In order to attain the other objectives we also performed immunocytochemical, immunohistochemical, RT-PCR, Western blot and fluorescence-activated cell sorting (FACS) analysis. Immunohistochemical analysis revealed the prominent association of iNOS with glioblastoma multiforme (GBM). Immunofluorescence analysis showed prominent expression of iNOS in glioma cells. MMP-9 and/or uPAR knockdown by respective shRNAs reduced iNOS expression in these glioma cells. RT-PCR analysis revealed elevated iNOS mRNA expression in either MMP-9 or uPAR overexpressed glioma cells. The migration potential of MMP-9- and/or uPAR-overexpressed U251 glioma cells was significantly inhibited after treatment with L-NAME, an inhibitor of iNOS. Similarly, a significant inhibition of the invasion potential of the control or MMP-9/uPAR-overexpressed glioma cells was noticed after L-NAME treatment. A prominent reduction of iNOS expression was observed in the tumor regions of nude mice brains, which were injected with 5310 glioma cells, after MMP-9 and/or uPAR knockdown. Protein expressions of cSrc, phosphoSrc and p

  17. A combination of desmopressin and docetaxel inhibit cell proliferation and invasion mediated by urokinase-type plasminogen activator (uPA) in human prostate cancer cells

    Background: This study was designed to assess the effectiveness of a combination treatment using both desmopressin and docetaxel in prostate cancer treatment. Desmopressin is a well-known synthetic analogue of the antidiuretic hormone vasopressin. It has recently been demonstrated to inhibit tumor progression and metastasis in in vivo models. Docetaxel is widely used for the treatment of castration resistant prostate cancer (CRPC) patients. However, durable responses have been uncommon to date. In this study, we investigated the anti-tumor effect of desmopressin in combination with docetaxel in vitro and in vivo. Methods: Two prostate cancer cells (PC3, LNCaP) were treated with different concentrations of desmopressin alone, docetaxel alone, and a combination of desmopressin and docetaxel. Cell proliferation was determined by MTS assay. The anti-invasive and anti-migration potential of desmopressin and in combination with docetaxel were examined by wound healing assay, migration chamber assay, and matrigel invasion assay. Results: The combination of desmopressin and docetaxel resulted in a significant inhibition of PC3 and LNCaP cell proliferation (p < 0.01). Additionally, cell migration and invasion were also inhibited by the combination when compared to that of either treatment alone in PC3 cells (p < 0.01). The anti-tumor effect of this combination treatment was associated with down-regulation of both urokinase-type plasminogen activator (uPA) and matrix metalloproteinase (MMP-2 and MMP-9) in PC3 cells. Conclusions: We are the first to elucidate the anti-tumor and anti-metastatic potential of desmopressin in combination with docetaxel in a prostate cancer model via the uPA-MMP pathway. Our finding could potentially contribute to the therapeutic profile of desmopressin and enhance the efficacy of docetaxel based treatment for CRPC. - Highlights: • Desmopressin inhibits cell proliferation in prostate cancer cells. • The expression of cyclin A and CDK2

  18. Prognostic significance of urokinase plasminogen activator and plasminogen activator inhibitor-1 mRNA expression in lymph node- and hormone receptor-positive breast cancer

    One of the most thoroughly studied systems in relation to its prognostic relevance in patients with breast cancer, is the plasminogen activation system that comprises of, among others, the urokinase Plasminogen Activator (uPA) and its main inhibitor, the Plasminogen Activator Inhibitor-1 (PAI-1). In this study, we investigated the prognostic value of uPA and PAI-1 at the mRNA level in lymph node- and hormone receptor-positive breast cancer. The study included a retrospective series of 87 patients with hormone-receptor positive and axillary lymph node-positive breast cancer. All patients received radiotherapy, adjuvant anthracycline-based chemotherapy and five years of tamoxifen treatment. The median patient age was 54 and the median follow-up time was 79 months. Distant relapse occurred in 30 patients and 22 patients died from breast cancer during follow-up. We investigated the prognostic value of uPA and PAI-1 at the mRNA level as measured by real-time quantitative RT-PCR. uPA and PAI-1 gene expression was not found to be correlated with any of the established clinical and pathological factors. Metastasis-free Survival (MFS) and Breast Cancer specific Survival (BCS) were significantly shorter in patients expressing high levels of PAI-1 mRNA (p < 0.0001; p < 0.0001; respectively). In Cox multivariate analysis, the level of PAI-1 mRNA appeared to be the strongest prognostic factor for MFS (Hazard Ratio (HR) = 10.12; p = 0.0002) and for BCS (HR = 13.17; p = 0.0003). Furthermore, uPA gene expression was not significantly associated neither with MFS (p = 0.41) nor with BCS (p = 0.19). In a Cox-multivariate regression analysis, uPA expression did not demonstrate significant independent prognostic value. These findings indicate that high PAI-1 mRNA expression represents a strong and independent unfavorable prognostic factor for the development of metastases and for breast cancer specific survival in a population of hormone receptor- and lymph node-positive breast cancer

  19. A Comparative Typology of Pension Regimes

    Arjan Soede; Cok Vrooman

    2008-01-01

    This report presents an empirical typology of pension regimes in the European Union, the US, Canada, Australia and Norway. The categorisation is based on 34 quantitative and qualitative characteristics of the mandatory parts of the pension systems in these countries. The empirical analysis shows tha

  20. REGIME ALIMENTAR DE GIRINOS DE RÃ TOURO

    José Teixeira de Seixas Filho

    2013-03-01

    Full Text Available We used 525 bullfrog tadpoles, distributed into 15 boxes with one tadpole per liter. The water was daily renewal (200%. The feeding regimes were constituted of five arrangements using seven commercial rations with levels of 22, 28, 32, 36, 40, 45 and 55% of crude protein (CP, supplied every 15 days. We used a random blocks design, subdivided plots with three replications. The subplots were constituted of five biometries: at the beginning of the experiment, at 15, 30, 45, and 60 days. The tadpoles submitted to feeding regime FR1, with 22, 32, 36, and 40% of CP, presented the highest consumption and the same performance as the others. Tadpoles that received FR5, with 40, 45, 45 and 50% of CP, presented greater weight, however, they showed smaller weight gain, greater consumption, conversion and mortality, indicating that this regime is expensive and not adequate for management. Feeding regimes FR2, with 28, 32, 36 and 40% of CP, and FR3, with 32, 36, 40 and 45% of CP, were more adjusted to this kind of management. Animals in all treatments showed higher performance that animals fed traditionally.

  1. Estimation in autoregressive models with Markov regime

    Ríos, Ricardo; Rodríguez, Luis

    2005-01-01

    In this paper we derive the consistency of the penalized likelihood method for the number state of the hidden Markov chain in autoregressive models with Markov regimen. Using a SAEM type algorithm to estimate the models parameters. We test the null hypothesis of hidden Markov Model against an autoregressive process with Markov regime.

  2. Optimal dividend distribution under Markov regime switching

    Z. Jiang; M. Pistorius

    2012-01-01

    We investigate the problem of optimal dividend distribution for a company in the presence of regime shifts. We consider a company whose cumulative net revenues evolve as a Brownian motion with positive drift that is modulated by a finite state Markov chain, and model the discount rate as a determini

  3. European welfare regimes: Political orientations versus poverty

    Josifidis Kosta

    2011-01-01

    Full Text Available This inquiry analyzes how political orientations shape welfare states and labour market institutions when seeking to reduce poverty. In order to identify effects of these two key variables, we conduct a panel regression analysis that includes two poverty measures: poverty rates before and after social spending. This inquiry considers 14 EU countries, and in the period from 1995 to 2008, which are grouped according to welfare state regimes. We consider Social Democratic, Corporatist, Mediterranean and Liberal welfare state regimes. Panel regression results indicate that political orientation engenders no significant statistically measurable effects on poverty rates before social spending. Effects register, however, as significant when considering poverty rates after social spending. With respect to the first set of results, we advance two key explanations. First, we note a longer period of time is necessary in order to observe actual effects of political orientation on market generated poverty. Second, political parties with their respective programs do not register as influential enough to solve social problems related to income distribution when taken alone. Influences register as indirect and are expressed through changes in employment rates and social spending. The second set of results support the hypothesis that a selected political regime does indeed contribute to poverty reduction. In sum, political orientation and political regime does indeed affect poverty through welfare state institutions, as well as through labour market institutions.

  4. Early detection of ecosystem regime shifts

    Lindegren, Martin; Dakos, Vasilis; Groeger, Joachim P.; Gardmark, Anna; Kornilovs, Georgs; Otto, Saskia A.; Moellmann, Christian

    2012-01-01

    methods may have limited utility in ecosystem-based management as they show no or weak potential for early-warning. We therefore propose a multiple method approach for early detection of ecosystem regime shifts in monitoring data that may be useful in informing timely management actions in the face of...

  5. Quality Regimes in Agro-Food Industries

    Staricco, Juan Ignacio; Ponte, Stefano

    2015-01-01

    In this article, we examine the transformative potential of changing quality regimes in agro-food industries through the analysis of whether Fair Trade wine in Argentina provides a meaningful economic alternative that goes beyond the impact it has on direct beneficiaries. The wine sector has a long...

  6. Radiative effects of global MODIS cloud regimes

    Oreopoulos, Lazaros; Cho, Nayeong; Lee, Dongmin; Kato, Seiji

    2016-03-01

    We update previously published Moderate Resolution Imaging Spectroradiometer (MODIS) global cloud regimes (CRs) using the latest MODIS cloud retrievals in the Collection 6 data set. We implement a slightly different derivation method, investigate the composition of the regimes, and then proceed to examine several aspects of CR radiative appearance with the aid of various radiative flux data sets. Our results clearly show that the CRs are radiatively distinct in terms of shortwave, longwave, and their combined (total) cloud radiative effect. We show that we can clearly distinguish regimes based on whether they radiatively cool or warm the atmosphere, and thanks to radiative heating profiles, to discern the vertical distribution of cooling and warming. Terra and Aqua comparisons provide information about the degree to which morning and afternoon occurrences of regimes affect the symmetry of CR radiative contribution. We examine how the radiative discrepancies among multiple irradiance data sets suffering from imperfect spatiotemporal matching depend on CR and whether they are therefore related to the complexity of cloud structure, its interpretation by different observational systems, and its subsequent representation in radiative transfer calculations.

  7. An emissions trading regime for Canada

    In 1998, over twelve papers were published on emissions trading regimes in Canada by the National Round Table on the Environment and the Economy (NRTEE), a federal government agency whose members represent stakeholders as varied as business, environmental groups, academics, aboriginal groups and others. One of the recommendations that emerged was for the computer modelling of the possibilities that had been identified for a domestic trading regime in Canada for greenhouse gases. It is unclear whether the modelling was ever performed as the file was taken over by the Finance Department under the umbrella of a special emission trading table that examined Canada's commitment under the Kyoto Protocol. The author examined questions pertaining to whether a domestic trading regime is essential, and what its characteristics should be in case it was deemed essential or advisable to have one. The upstream versus downstream application was looked at, as well as grand-fathering versus auction. Provincial issues were then addressed, followed by meshing with a credit system. International systems were reviewed. Early action was discussed, whereby an emitter seeks credit for action taken toward reductions since the original reference year of 1990. The case of emitters having bought or sold permits since the original reference years will also want those trades recognized under a trading regime. The author indicated that it seems probable that an emission trading system will eventually be implemented and that a debate on the issue should be initiated early

  8. The global safety regime - Setting the stage

    Full text: The existing global safety regime has arisen from the exercise of sovereign authority, with an overlay of voluntary international cooperation from a network of international and regional organizations and intergovernmental agreements. This system has, in the main, served us well. For several reasons, the time is ripe to consider the desired shape of a future global safety regime and to take steps to achieve it. First, every nation's reliance on nuclear power is hostage to some extent to safety performance elsewhere in the world because of the effects on public attitudes and hence there is an interest in ensuring achievement of common standards. Second, the world is increasingly interdependent and the vendors of nuclear power plants seek to market their products throughout the globe. Efficiency would arise from the avoidance of needless differences in approach that require custom modifications from country to country. Finally, we have much to learn from each other and a common effort would strengthen us all. Such an effort might also serve to enhance public confidence. Some possible characteristics of such a regime can be identified. The regime should reflect a global consensus on the level of safety that should be achieved. There should be sufficient standardization of approach so that expertise and equipment can be used everywhere without significant modification. There should be efforts to ensure a fundamental commitment to safety and the encouragement of a safety culture. And there should be efforts to adopt more widely the best regulatory practices, recognizing that some modifications in approach may be necessary to reflect each nation's legal and social culture. At the same type, the regime should have the characteristics of flexibility, transparency, stability, practicality, and encouragement of competence. (author)

  9. The global safety regime - Setting the stage

    The existing global safety regime has arisen from the exercise of sovereign authority, with an overlay of voluntary international cooperation from a network of international and regional organizations and intergovernmental agreements. This system has, in the main, served us well. For several reasons, the time is ripe to consider the desired shape of a future global safety regime and to take steps to achieve it. First, every nation's reliance on nuclear power is hostage to some extent to safety performance elsewhere in the world because of the effects on public attitudes and hence there is an interest in ensuring achievement of common standards. Second, the world is increasingly interdependent and the vendors of nuclear power plants seek to market their products throughout the globe. Efficiency would arise from the avoidance of needless differences in approach that require custom modifications from country to country. Finally, we have much to learn from each other and a common effort would strengthen us all. Such an effort might also serve to enhance public confidence. Some possible characteristics of such a regime can be identified. The regime should reflect a global consensus on the level of safety that should be achieved. There should be sufficient standardization of approach so that expertise and equipment can be used everywhere without significant modification. There should be efforts to ensure a fundamental commitment to safety and the encouragement of a safety culture. And there should be efforts to adopt more widely the best regulatory practices, recognizing that some modifications in approach may be necessary to reflect each nation's legal and social culture. At the same type, the regime should have the characteristics of flexibility, transparency, stability, practicality, and encouragement of competence. (author)

  10. De facto and official exchange rate regimes in transition economies

    von Hagen, Jürgen; Zhou, Jizhong

    2002-01-01

    This paper provides an empirical investigation on the discrepancies between official exchange rate regimes and de facto exchange rate policies in transition economies. Since official and de facto regime choices are not independent of each other, we adopt a bivariate probit model to describe the joint determination of the two regime choices. After finding the important determinants of both regime choices, we use a univariate probit model to describe the determination of regime discrepancies. W...

  11. The Effect of Market Regimes on Style Allocation

    Ammann, Manuel; Verhofen, Michael

    2006-01-01

    We analyse time-varying risk premia and the implications for portfolio choice. Using Markov Chain Monte Carlo (MCMC) methods, we estimate a multivariate regime-switching model for the Carhart (1997) four factor model. We find two clearly separable Regimes with different mean returns, volatilities and correlations. In the High-Variance Regime, only value stocks deliver a good performance, whereas in the Low-Variance Regime, the market portfolio and momentum stocks promise high returns. Regime-...

  12. Regime shifts in the anthropocene: drivers, risks, and resilience.

    Rocha, Juan Carlos; Peterson, Garry D; Biggs, Reinette

    2015-01-01

    Many ecosystems can experience regime shifts: surprising, large and persistent changes in the function and structure of ecosystems. Assessing whether continued global change will lead to further regime shifts, or has the potential to trigger cascading regime shifts has been a central question in global change policy. Addressing this issue has, however, been hampered by the focus of regime shift research on specific cases and types of regime shifts. To systematically assess the global risk of regime shifts we conducted a comparative analysis of 25 generic types of regime shifts across marine, terrestrial and polar systems; identifying their drivers, and impacts on ecosystem services. Our results show that the drivers of regime shifts are diverse and co-occur strongly, which suggests that continued global change can be expected to synchronously increase the risk of multiple regime shifts. Furthermore, many regime shift drivers are related to climate change and food production, whose links to the continued expansion of human activities makes them difficult to limit. Because many regime shifts can amplify the drivers of other regime shifts, continued global change can also be expected to increase the risk of cascading regime shifts. Nevertheless, the variety of scales at which regime shift drivers operate provides opportunities for reducing the risk of many types of regime shifts by addressing local or regional drivers, even in the absence of rapid reduction of global drivers. PMID:26267896

  13. Online recognition of the multiphase flow regime

    2008-01-01

    The key reasons that the present method cannot be used to solve the industrial multi- phase flow pattern recognition are clarified firstly. The prerequisite to realize the online recognition is proposed and recognition rules for partial flow pattern are obtained based on the massive experimental data. The standard templates for every flow regime feature are calculated with self-organization cluster algorithm. The multi-sensor data fusion method is proposed to realize the online recognition of multiphase flow regime with the pressure and differential pressure signals, which overcomes the severe influence of fluid flow velocity and the oil fraction on the recognition. The online recognition method is tested in the practice, which has less than 10 percent measurement error. The method takes advantages of high confidence, good fault tolerance and less requirement of single sensor performance.

  14. Online recognition of the multiphase flow regime

    BAI BoFeng; ZHANG ShaoJun; ZHAO Liang; ZHANG XiMin; GUO LieJin

    2008-01-01

    The key reasons that the present method cannot be used to solve the industrial multi-phase flow pattern recognition are clarified firstly. The prerequisite to realize the online recognition is proposed and recognition rules for partial flow pattern are obtained based on the massive experimental data. The standard templates for every flow regime feature are calculated with self-organization cluster algorithm. The multi-sensor data fusion method is proposed to realize the online recognition of multiphase flow regime with the pressure and differential pressure signals, which overcomes the severe influence of fluid flow velocity and the oil fraction on the recognition. The online recognition method is tested in the practice, which has less than 10 percent measurement error. The method takes advantages of high confidence, good fault tolerance and less requirement of single sensor performance.

  15. Recent Activities on Global Nuclear Safety Regime

    Recently, rapid progress on the globalization of the nuclear safety issues is being made in IAEA (International Atomic Energy Agency) and its member states. With the globalization, the need for international cooperation among international bodies and member states continues to grow for resolving these universal nuclear safety issues. Furthermore, the importance of strengthening the global nuclear safety regime is emphasized through various means, such as efforts in application of IAEA safety standards to all nuclear installations in the world and in strengthening the code of conduct and the convention on nuclear safety. In this regards, it is important for us to keep up with the activities related with the global nuclear safety regime as an IAEA member state and a leading country in nuclear safety regulation

  16. Progress towards a global nuclear liability regime

    As the Fukushima-Daiichi nuclear power plant accident brought a renewed focus to the discussion of international nuclear liability regimes, and notably the administration of a liability system to compensate damages resulting from a nuclear accident, this article recalls the background on existing international nuclear liability conventions (original and revised Paris and Vienna conventions), briefly indicates the common principles reflected in the international nuclear liability conventions. The author outlines the challenges in achieving a global nuclear liability regime, reports and comments the Japanese experience in handling compensation issues in the wake of the Fukushima accident. He gives an overview of recent developments: IAEA action plan on nuclear safety, action of the International Expert Group on Nuclear Liability (INLEX), CSC or Convention on Supplementary Compensation

  17. Regimes de espaço

    Eric Landowski

    2015-06-01

    Full Text Available Space Regimes - Based on a general model of interaction, the present analysis attempts to account for the diversity of the ways space may be apprehended in lived experience. It leads to the definition of four configurations that correspond to as many regimes of relation with the environing world. The conventional space of the circulation of values takes the archetypical shape of the net (incarnated today by Internet. The operational space is that of our dealing with objects within a material environment seen as a tissue of stable and intelligible relations. The experienced space of bodies’ movement, to which one may give as an emblem the spiral, expresses the dynamics of sensitive relationships between the self and the other. The existential space is that of our presence in a universe without boundaries, which no one can properly represent oneself but that nonetheless haunts art and thought: its figure is the abyss.

  18. Dominant takeover regimes for genetic algorithms

    Noever, David; Baskaran, Subbiah

    1995-01-01

    The genetic algorithm (GA) is a machine-based optimization routine which connects evolutionary learning to natural genetic laws. The present work addresses the problem of obtaining the dominant takeover regimes in the GA dynamics. Estimated GA run times are computed for slow and fast convergence in the limits of high and low fitness ratios. Using Euler's device for obtaining partial sums in closed forms, the result relaxes the previously held requirements for long time limits. Analytical solution reveal that appropriately accelerated regimes can mark the ascendancy of the most fit solution. In virtually all cases, the weak (logarithmic) dependence of convergence time on problem size demonstrates the potential for the GA to solve large N-P complete problems.

  19. Deuteron structure in the deep inelastic regime

    Canal, C A Garcìa; Vento, V

    2016-01-01

    We study nuclear effects on the deuteron in the deep inelastic regime using the newest available data analyzing their $Q^2$ dependence. We conclude that precise EMC ratios for large $Q^2$ (> 30, GeV$^2$) cannot be obtained without considering these nuclear effects. For this purpose we use a scheme which parametrizes these effects in a simple manner and compare our results with other recent proposals.

  20. Financial Fragility and the Exchange Rate Regime

    Chang, Roberto; Velasco, Andres

    1998-01-01

    We study financial fragility, exchange rate crises and monetary policy in an open economy model in which banks are maturity transformers as in Diamond-Dybvig. The banking system, the exchange rate regime, and central bank credit policy are seen as parts of a mechanism intended to maximize social welfare; if the mechanism fails, banking crises and speculative attacks become possible. We compare currency boards, fixed rate and flexible rates, with and without a lender of last resort. A currency...

  1. Tax compliance under tax regime changes

    Heinemann, Friedrich; Kocher, Martin G.

    2010-01-01

    In this paper we focus on the compliance effects of tax regime changes. According to the economic model of tax evasion, a tax reform should affect compliance through its impact on tax rates and incentives. Our findings demonstrate the importance of at least two further effects not covered by the traditional model: First, reform losers tend to evade more taxes after the reform. Second, a reform from a proportionate to a progressive system decreases compliance compared to a switch in the revers...

  2. Law and Courts in Authoritarian Regimes

    Moustafa, Tamir

    2014-01-01

    "Once regarded as mere pawns of their regimes, courts in authoraitarian states are now the subject of considerable attention within the field of comparative judicial politics.  New research examines the ways in which law and courts are deployed as instruments of governance, how they structure state-society contention, and the circumstances in which courts are transformed into sites of active resistance.  This new body of research constitutes an emergent field of inquiry, while simul...

  3. Exchange Rate Regimes and International Business Cycles

    Theptida Sopraseuth

    2003-01-01

    This paper investigates the impact of exchange rate regimes on international business cycles and focuses on the consequences of membership to the European Monetary System. The volatility puzzle uncovered by Baxter and Stockman [1989, Journal of Monetary Economics 23, 377–401] after assessing the consequences of the Bretton Woods system turns out to be a robust stylized fact: real and nominal exchange rates display a higher volatility under floating rates while the variability of macroeconom...

  4. Snowpack regimes of the Western United States

    Trujillo, Ernesto; Molotch, Noah P.

    2014-01-01

    Snow accumulation and melt patterns play a significant role in the water, energy, carbon, and nutrient cycles in the montane environments of the Western United States. Recent studies have illustrated that changes in the snow/rainfall apportionments and snow accumulation and melt patterns may occur as a consequence of changes in climate in the region. In order to understand how these changes may affect the snow regimes of the region, the current characteristics of the snow accumulation and mel...

  5. Financing welfare regimes: mapping heterogeneous revenue structures

    Gough, Ian; Abu-Sharkh, Miriam

    2011-01-01

    This article studies how the composition of public revenues in terms of sources (such as taxation, social insurance contributions, mineral rents, foreign aid) is associated with different welfare regimes and social policy outcomes. It is divided into two halves: a review of literature and research, and a cross-national data analysis. The first half reviews literature on the emergence of tax and revenue systems in the West, and on the relevance of these frameworks and findings to developing co...

  6. The Forex Regime and EMU Expansion

    Foreest, P.W.; de Vries, Casper

    2002-01-01

    This paper provides evidence that the choice of the foreign exchange regime is not of first order importance for achieving high output growth. It is argued that due to the forward looking nature of the foreign exchange market, exchange rate stability hinges on the current and anticipated coherency of monetary and fiscal policies. We demonstrate this empirically on a panel including potential EMU accession countries. By means of rank regression analysis we uncover the partial links across the ...

  7. Dynamic Regime of Ignition of Solid Propellant

    Zolotorev Nikolay

    2016-01-01

    Full Text Available This article presents a dynamic regime of exposure of the radiant flux on the sample of gun-cotton. Obtained time the ignition of gun-cotton in the heating conditions of increasing heat flux in the range from 0.2 W/cm2 to 22 W/cm2. A comparison of the delay times of the ignition when heated variable and constant heat flux.

  8. Exchange Rate Regimes, Location, and Specialization

    Luca Antonio Ricci

    2006-01-01

    This paper investigates the effects of fixed versus flexible exchange rates on firms' location choices and on countries' specialization patterns. In a two-country, twodifferentiated-goods monetary model, uncertainty arises after wages are set and prices are optimally chosen. The paper shows that countries are more specialized under flexible than fixed rates, which indicates that the pattern of specialization is not uniquely defined by trade models but also depends on the exchange rate regime....

  9. Corruption in Privatization and Governance Regimes

    Maria Cristina Molinari

    2011-01-01

    We consider the choice to privatize the provision of a public good in a hierarchical model with three layers: a Central Government, a decentralized agency and a (private or public) manager. In a good governance regime the privatization can be devolved upon the decentralized agency while it cannot when the governance is bad. There are two types of information asymmetries: managers are privately informed of their efficiency in reducing costs (and quality) and only the decentralized agency knows...

  10. Marginal Mean Models for Dynamic Regimes

    Murphy, S A; Laan, M.J. van der; Robins, J.M.

    2001-01-01

    A dynamic treatment regime is a list of rules for how the level of treatment will be tailored through time to an individual’s changing severity. In general, individuals who receive the highest level of treatment are the individuals with the greatest severity and need for treatment. Thus there is planned selection of the treatment dose. In addition to the planned selection mandated by the treatment rules, the use of staff judgment results in unplanned selection of the treatment level. Given ob...

  11. Tax incentive regimes: models and research methods

    Sokolovska, Olena; Sokolovskyi, Dmytro

    2014-01-01

    Paper deals with problem of effectiveness of tax incentive regimes. Support of priority industries remains currently important for transition economies. At once, such countries more often than developed ones face the imperfection of tax incentive mechanisms. Notably, such problems are common to Ukrainian and Moldavian economies, where the legislative support or its implementation in the area of preferential taxation of innovative enterprises which contribute essentially to sustainable develop...

  12. Taxation, corruption and the exchange rate regime

    Hefeker, Carsten

    2008-01-01

    The paper analyzes the relation between institutional quality, such as corruption, in a country and its monetary regime. It is shown that a credibly fixed exchange rate to a low inflation country, like a currency board, can reduce corruption and improve the fiscal system. A monetary union, however, has ambiguous effects. I find that that there is convergence between countries with regard to the level of corruption.

  13. Monetary policy regimes and the Nordic model

    Schewe, Theo

    2015-01-01

    In many contexts, the Nordic countries are regarded as a natural bloc of nations that have common political, cultural and economic characteristics. Open to globalisation, the small Nordic countries are exposed to strong global competition and cyclical influences. Therefore, the basis of the model may be to combine collective risk sharing and openness to globalisation. Studies of the Nordic model do not focus on the question whether the monetary policy regime is a constitutive element of the m...

  14. Energy regime choices: nuclear or not?

    Elliott, David

    2006-01-01

    The energy system in industrialized countries is changing in what can be seen as an example of the technological regime change, reflecting a wider shift towards environmentally sustain-able technology which may impact on all sectors in the economy. In recent years, the emphasis in the UK's power generation system has increasingly been on smaller scale power plants, combined cycle gas turbine plants and wind farms of the order of 20-100 megawatts instead of giant gigawatt coal and nuclear p...

  15. Nonlinear diffusion regimes in stochastic magnetic fields

    The transport of collisional particles in stochastic magnetic fields is studied using the decorrelation trajectory method. The nonlinear effect of magnetic line trapping is considered together with particle collisions. The running diffusion coefficient is determined for arbitrary values of the statistical parameters of the stochastic magnetic field and of the collisional velocity. New diffusion regimes are found in the conditions for which the trapping of magnetic field lines is effective. (author)

  16. APPLICABLE LAW PRINCIPLES FOR MATRIMONIAL PROPERTY REGIMES

    Andreea-Lorena CODREANU

    2014-01-01

    More and more often, marriages are concluded between people of different nationalities, which brings an extraneous dimension to the legal status and matrimonial property regime jurisdiction conflict. Many European states (among which Romania, through its new Civil Code) offer the advantage of clauses that could be used by any of the spouses, with regard to the applicable law. In case of divorce, consequences differ with the applicable law. Spouses who divorce and divide their assets based on ...

  17. Supersonic free jet, molecular free regime

    The structure of the free jet emitted by a converging nozzle as obtained by the method of characteristics by Ashkenas and Sherman is described in details. In particular the dependence of the field variable by the distance from the nozzle is given. The transition from continuum to molecular free regime is then considered and the sudden freeze approximation is introduced. The processing of monoatomic and polyatomic gasses is also considered

  18. Primary Qualification of Matrimonial Regime Notion

    Nadia Cerasela ANITEI

    2012-12-01

    Full Text Available By adopting Law no. 287 of July 17, 2009 on the Civil Code republished by Law no. 71/2011 the new Civil Code is subject to the modern legislations tendencies to create a triple balance in terms of property relations between spouses by means of the matrimonial property regimes established:1. between spouses: through the appearance of matrimonial agreements, which have led to the adoption of more flexible legal rules which allow spouses a certain freedom to choose the regime of patrimonial relations between them;2. within the family: to protect the interests of the family, they resorted to mandatory rules providing for limitations and prohibitions (art.321-322 on the family home – a new notion in the Romanian law, art.316 on the acts of disposal seriously threatening family interests;3. between family and society-third parties: by establishing formal requirements of legal acts, including matrimonial agreements to be concluded by notarial act that must be given to publicity afterwards.Also, considering the fact that Romania is a member state of the European Union and that more and more Romanian people are living abroad or marry foreigners, we consider the harmonization of legislation in order to determine the law applicable to such relationships as a highly important requirement.Given these issues we will devote the present study: to primary qualification of the matrimonial regime notion.

  19. Constructing an interdisciplinary flow regime recommendation

    Bartholow, J.M.

    2010-01-01

    It is generally agreed that river rehabilitation most often relies on restoring a more natural flow regime, but credibly defining the desired regime can be problematic. I combined four distinct methods to develop and refine month-by-month and event-based flow recommendations to protect and partially restore the ecological integrity of the Cache la Poudre River through Fort Collins, Colorado. A statistical hydrologic approach was used to summarize the river's natural flow regime and set provisional monthly flow targets at levels that were historically exceeded 75% of the time. These preliminary monthly targets were supplemented using results from three Poudre-specific disciplinary studies. A substrate maintenance flow model was used to better define the high flows needed to flush accumulated sediment from the river's channel and help sustain the riparian zone in this snowmelt-dominated river. A hydraulic/habitat model and a water temperature model were both used to better define the minimum flows necessary to maintain a thriving cool water fishery. The result is a range of recommended monthly flows and daily flow guidance illustrating the advantage of combining a wide range of available disciplinary information, supplemented by judgment based on ecological principles and a general understanding of river ecosystems, in a highly altered, working river. ?? 2010 American Water Resources Association.

  20. The kinetic regime of the Vicsek model

    Chepizhko, A. A.; Kulinskii, V. L.

    2009-12-01

    We consider the dynamics of the system of self-propelling particles modeled via the Vicsek algorithm in continuum time limit. It is shown that the alignment process for the velocities can be subdivided into two regimes: "fast" kinetic and "slow" hydrodynamic ones. In fast kinetic regime the alignment of the particle velocity to the local neighborhood takes place with characteristic relaxation time. So, that the bigger regions arise with the velocity alignment. These regions align their velocities thus giving rise to hydrodynamic regime of the dynamics. We propose the mean-field-like approach in which we take into account the correlations between density and velocity. The comparison of the theoretical predictions with the numerical simulations is given. The relation between Vicsek model in the zero velocity limit and the Kuramoto model is stated. The mean-field approach accounting for the dynamic change of the neighborhood is proposed. The nature of the discontinuity of the dependence of the order parameter in case of vectorial noise revealed in Gregorie and Chaite, Phys. Rev. Lett., 92, 025702 (2004) is discussed and the explanation of it is proposed.

  1. QCD in the δ-regime

    The δ-regime of QCD is characterised by light quarks in a small spatial box, but a large extent in (Euclidean) time. In this setting a specific variant of chiral perturbation theory - the δ-expansion - applies, based on a quantum mechanical treatment of the quasi onedimensional system. In particular, for vanishing quark masses one obtains a residual pion mass MRπ, which has been computed to the third order in the δ-expansion. A comparison with numerical measurements of this residual mass allows for a new determination of some Low Energy Constants, which appear in the chiral Lagrangian. We first review the attempts to simulate 2-flavour QCD directly in the δ-regime. This is very tedious, but results compatible with the predictions for MRπ have been obtained. Then we show that an extrapolation of pion masses measured in a larger volume towards the δ-regime leads to good agreement with the theoretical predictions. From those results, we also extract a value for the (controversial) sub-leading Low Energy Constant anti l3. (orig.)

  2. QCD in the delta-Regime

    Bietenholz, W; Gockeler, M; Horsley, R; Nakamura, Y; Pleiter, D; Rakow, P E L; Schierholz, G; Zanotti, J M

    2011-01-01

    The delta-regime of QCD is characterised by light quarks in a small spatial box, but a large extent in (Euclidean) time. In this setting a specific variant of chiral perturbation theory - the delta-expansion - applies, based on a quantum mechanical treatment of the quasi one-dimensional system. In particular, for vanishing quark masses one obtains a residual pion mass M_pi^R, which has been computed to the third order in the delta-expansion. A comparison with numerical measurements of this residual mass allows for a new determination of some Low Energy Constants, which appear in the chiral Lagrangian. We first review the attempts to simulate 2-flavour QCD directly in the delta-regime. This is very tedious, but results compatible with the predictions for M_pi^R have been obtained. Then we show that an extrapolation of pion masses measured in a larger volume towards the delta-regime leads to good agreement with the theoretical predictions. From those results, we also extract a value for the (controversial) sub-...

  3. QCD in the δ-Regime

    The (δ-regime of QCD is characterised by light quarks in a small spatial box, but a large extent in (Euclidean) time. In this setting a specific variant of chiral perturbation theory - the (δ-expansion - applies, based on a quantum mechanical treatment of the quasi one-dimensional system. In particular, for vanishing quark masses one obtains a residual pion mass MRπ, which has been computed to the third order in the δ-expansion. A comparison with numerical measurements of this residual mass allows for a new determination of some Low Energy Constants, which appear in the chiral Lagrangian. We first review the attempts to simulate 2-flavour QCD directly in the δ-regime. This is very tedious, but results compatible with the predictions for MRπ, have been obtained. Then we show that an extrapolation of pion masses measured in a larger volume towards the (δ-regime leads to good agreement with the theoretical predictions. From those results, we also extract a value for the (controversial) sub-leading Low Energy Constant l-bar3.

  4. Interpolation between the epsilon and p regimes

    Aoki, Sinya

    2011-01-01

    We reconsider chiral perturbation theory in a finite volume and develop a new computational scheme which smoothly interpolates the conventional epsilon and p regimes. The counting rule is kept essentially the same as in the p expansion. The zero-momentum modes of Nambu-Goldstone bosons are, however, treated separately and partly integrated out to all orders as in the epsilon expansion. In this new scheme, the theory remains infra-red finite even in the chiral limit, while the chiral-logarithmic effects are kept present. We calculate the two-point function in the pseudoscalar channel and show that the correlator has a constant contribution in addition to the conventional hyperbolic cosine function of time t. This constant term rapidly disappears in the p regime but it is indispensable for a smooth convergence of the formula to the epsilon regime result. Our calculation is useful to precisely estimate the finite volume effects in lattice QCD simulations on the pion mass Mpi and kaon mass MK, as well as their de...

  5. British Columbia's new coalbed methane royalty regime

    The British Columbia Ministry of Energy and Mines is promoting the development of the coalbed methane (CBM) industry in the province in order to make CBM a viable and competitive investment option for industry. It is establishing a regulatory and fiscal regime for CBM development. Issues of concern regarding CBM development include water production, gas production rates, well numbers, and marginal economics. The features of the CBM royalty regime include a new producer cost of service allowance, the creation of a CBM royalty tax bank to collect excess PCOS allowances, and a royalty tax credit for wells drilled by the end of February, 2004. The marginal well adjustment factor threshold has been raised from 180 mcf per day to 600 mcf per day for CBM only. It was noted that royalties will probably not be payable for several years following the first commercial well because royalties are very depending on capital and operating costs, local infrastructure and price. Royalty regimes cannot save CBM from low gas prices, poor resources or economics. 2 figs

  6. JT-60U high performance regimes

    High performance regimes of JT-60U plasmas are presented with an emphasis upon the results from the use of a semi-closed pumped divertor with W-shaped geometry. Plasma performance in transient and quasi steady states has been significantly improved in reversed shear and high-β p regimes. The reversed shear regime elevated an equivalent QDTeq transiently up to 1.25(nD(0)τE Ti(0) = 8.6x1020m-3 · s · keV) in a reactor-relevant thermonuclear dominant regime. Long sustainment of enhanced confinement with internal transport barriers (ITBs) with a fully non-inductive current drive in a reversed shear discharge was successfully demonstrated with LH wave injection. Performance sustainment has been extended in the high-β p regime with a high triangularity achieving a long sustainment of plasma conditions equivalent to QDTeq ∼ 0.16(nD(0)τE Ti(0) ∼ 1.4x1020m-3 · s · keV) for ∼ 4.5 s with a large non-inductive current drive fraction of 60-70% of the plasma current. Thermal and particle transport analyses show significant reduction of thermal and particle diffusivities around ITB resulting in a strong Er shear in the ITB region. The W-shaped divertor is effective for He ash exhaust demonstrating steady exhaust capability of τHe*/τE ∼ 3 - 10 in support of ITER. Suppression of neutral back flow and chemical sputtering effect have been observed while MARFE onset density is rather decreased. Negative-ion based neutral beam injection (N-NBI) experiments have created a clear H-mode transition. Enhanced ionization cross-section due to multi-step ionization processes was confirmed as theoretically predicted. A current density profile driven by N-NBI is measured in a good agreement with theoretical prediction. N-NBI induced TAE modes characterized as persistent and bursting oscillations have been observed from a low hot beta of h > ∼ 0.1 - 0.2% without a significant loss of fast ions. (author)

  7. Localization and distribution of tissue type and urokinase type plasminogen activators and their inhibitors Type 1 and 2 in human and rhesus monkey fetal membranes.

    Liu, Y X; Hu, Z Y; Liu, K; Byrne, S; Zou, R J; Ny, T; d'Lacey, C; Ockleford, C D

    1998-01-01

    Fetal membranes consist of 10 distinct layers including components of amnion, chorion and decidua, the latter being of maternal origin. They form mechanically integrated sheets capable of retaining amniotic fluid and play an essential role in protecting fetal growth and development in the pregnant uterus. The extracellular matrix, substrate for plasminogen activators (PAs), is an important supportive framework of the fetal membranes. Fetal membranes from women with preterm premature rupture of membranes may differ in their protease activity compared with normal membranes. To identify the presence of PAs and their inhibitors (PAI) and their possible role in the process of fetal membrane rupture, this study investigated the distribution and localization of both protein and mRNA for tissue (t) and urokinase (u) PA and their inhibitors type 1 (PAI-1) and type 2 (PAI-2) in amniochorion of human and rhesus monkey using conventional and confocal immunofluorescence microscopy. In situ hybridization analysis showed that the distribution and localization of mRNAs for tPA, uPA, PAI-1 and PAI-2 were similar in the fetal membranes of human and rhesus monkey; no obvious species difference was observed. Evidence of tPA mRNA was detected in amniotic epithelium, trophoblast cells and nearly all cells of the decidual layer. Strong expression of uPA mRNA was noted in the decidual cells which increased in intensity as the abscission point was approached. Weak staining in chorion laeve trophoblast was also detected. In situ hybridization experiments showed PAI-1 mRNA to be concentrated mainly in the decidual cells, some of which were interposed into the maternal-facing edge of the chorion laeve. Maximal labelling of the decidua occurred towards the zone of abscission. Weak expression of PAI-1 mRNA was also noted in some cells of the chorion laeve. The distribution of PAI-2 mRNA in amniochorion was also concentrated in the cells of the decidual layer, maximum expression of the mRNA was

  8. Flavonol-enriched fraction from Vaccinium macrocarpon fruit inhibits matrix metalloproteinase-2, matrix metalloproteinase-9 and urokinase-type plasminogen activator expression in human prostate cancer cells in vitro

    James MacPhee

    2014-11-01

    Full Text Available Background: Prostate cancer, amongst other cancer types has a genetic and environmental component, which can contribute to prostate cancer development and progression. Vaccinum macrocarpon (American cranberry is a botanical that contains several phytochemicals which have been suggested to play a role in preventing cardiovascular disease, cancer, and urinary tract infections as well as in the maintenance of oral health. Context and purpose of this study: This investigation evaluated the effects of a flavonolenriched fraction (FL from the American cranberry (Vaccinium macrocarpon containing quercetin and myricetin glycosides on matrix metalloproteinase (MMP and urokinase-type plasminogen activator (uPA activities and their associated regulatory proteins in DU145 human prostate cancer cells in vitro. Results: A flavonol-enriched fraction (FL was prepared from Vaccinium macrocarpon berries and the effect of this fraction on prostate cancer cell behaviour was assessed using biochemical and molecular approaches including cytotoxicity assays and Western blot analysis to determine protein expression. Cranberry FL decreased cellular viability of DU145 cells at a concentration of 25 ug/ml by 20% after 6 hours of treatment. Further investigations determined that associated with this cytotoxicity, cranberry FL decreases matrix metalloproteinase (MMP ( specifically MMP-2 and MMP-9 activity and urokinase plasminogen activator (uPA activity through effects on specific temporal MMP regulators and uPA regulators and by affecting either the phosphorylation status and/or expression of specific MAP kinase, PI-3 kinase, NF-kB and AP-1 pathway associated proteins. Conclusion: This study demonstrates, for the first time, the ability of Vaccinium macrocarpon flavonols to modulate cellular pathways associated with migration, invasion, and proliferation, suggesting that cranberry (Vaccinium macrocarpon is a viable candidate for further research as a natural product that

  9. Refrigeration. Two-Phase Flow. Flow Regimes and Pressure Drop

    Knudsen, Hans-Jørgen Høgaard

    2002-01-01

    The note gives the basic definitions used in two-phase flow. Flow regimes and flow regimes map are introduced. The different contributions to the pressure drop are stated together with an imperical correlation from the litterature....

  10. Laboratory Exploration of Multiple Zonal Jet Regimes

    Smith, C. A.; Speer, K. G.; Griffiths, R. W.

    2012-12-01

    The differentially heated, rotating annulus has classically been used to study wave interactions within a single, baroclinic jet. At high rotation rates, the baroclinic instability of the flow leads to a transition to a turbulent, eddy-dominated regime. In the presence of a topographic beta effect, the flow has been observed to produce multiple, meandering zonal jets that are qualitatively similar to those found in planetary atmospheres and in the Antarctic Circumpolar Current (ACC). Our study builds on previous annulus experiments [1] by making observations further within this new regime. We observe with PIV and other techniques how the structure of the flow responds to changes in various parameters such as tank geometry, gradient in the Coriolis parameter, rotation rate, and differential thermal forcing. By not employing the more typical direct forcing of small scales, but by applying a large scale forcing over the annulus gap width, this study allows the varying effects of eddy scale selection, enstrophy cascade, etc. to naturally generate flow that more closely resembles planetary atmospheres and the ACC. We seek nondimensional parameters that significantly control zonation in a real fluid. These observations will provide a metric for the comparison of various theoretical models for multiple zonal jet formation. Other properties of the jets, such as their migration, meandering, bifurcation, and merging, can also be observed in an idealized situation and compared to numerical simulations. Ultimately, this will aid the testing and development of sub-grid-scale parameterizations for the multiple zonal jet regime that remain robust in the face of multiple forcing parameters. [1] Wordsworth, R. D., Read, P. L., & Yamazaki, Y. H. (2008). Turbulence, waves, and jets in a differentially heated rotating annulus experiment Physics of Fluids, 20(12), 126602.Streak photograph of suspended particles visualizing the flow representative of multiple zonal jets

  11. Modeling water regime in a small watershed

    Vogel, T.; Tesař, Miroslav; Císlerová, M.

    Praha: Ústav pro hydrodynamiku AV ČR, 2003 - (Šír, M.; Lichner, Ľ.; Tesař, M.), s. 127-136 ISBN 80-02-01586-X. [Hydrologie půdy v malém povodí. Praha (CZ), 15.10.2003] R&D Projects: GA AV ČR IAA3060001 Institutional research plan: CEZ:AV0Z2060917 Keywords : Soil water regime * Subsurface runoff generation * Rainfall-runoff event Subject RIV: DA - Hydrology ; Limnology

  12. REVIEW OF REGIME THEORY OF ALLUVIAL CHANNELS

    2002-01-01

    One of the most important problems in river engineering is to determine a stable cross section geomenry and slope for an alluvial channel. This has been the subject of considerable research for about a century and continues to be of great practical interest. Lgnoring plan geometry, an alluvi-al channel can adjust its slope, depth and width, to develop a dynamic stable condition in which it can transport a certain a-mount of water and sediment. The brief history of regime the-ory and its new development are reviewed in this paper.

  13. Bose polarons in the strongly interacting regime

    Hu, Ming-Guang; van de Graaff, Michael; Kedar, Dhruv; Cornell, Eric; Jin, Deborah

    Impurities immersed in and interacting with a Bose-Einstein condensate (BEC) are predicted to form quasiparticle excitations called Bose polarons. I will present experimental evidence of Bose polarons in cold atoms obtained using radio-frequency spectroscopy to measure the excitation spectrum of fermionic 40K impurities interacting with a BEC of 87Rb atoms. We use an interspecies Feshbach resonance to tune the interactions between the impurities and the bosons, and we take data in the strongly interacting regime. This work is supported by NSF, NASA and NIST.

  14. The Tributary Regime in the oil sector

    The tributary regime of the oil sector, is framed by the fiscal crisis that the country, maxim if one keeps in mind the importance of the fiscal revenues originated in the exploitation of these resources in Colombia, so much for the tribute coming from the foreign investment of the sector, like for the utilities generated by ECOPETROL and its impact in the public finances. However in front of this focus, the strategic importance of maintaining the investment in hydrocarbons and the paper that the fiscal politics and the tributary politics for the sector should play in the future and they constitute the government's key pieces

  15. Autoritarismus in Peru : das Fujimori-Regime

    Crabtree, John

    2007-01-01

    The ten-years presidency of Alberto Fujimori in Peru is a classic example of the sort of hybrid democracy that emerged in various Latin American countries in the 1990s, combining a mixture of democratic and clearly non-democratic elements. Fujimori was one of the more autocratic Latin American leaders of the 1990s, he is being the only regime formally to break with pre-existing constitutional rules. Closely tied to the military and intelligence apparatus, his government involved a concentrati...

  16. Random nanolasing in the Anderson localized regime

    Liu, Jin; Garcia, P. D.; Ek, Sara;

    2014-01-01

    multiple scattering. The applicability of random lasers has been limited due to multidirectional emission, lack of tunability, and strong mode competition with chaotic fluctuations due to a weak mode confinement. The regime of Anderson localization of light has been proposed for obtaining stable multimode...... random lasing, and initial work concerned macroscopic one-dimensional layered media. Here, we demonstrate on-chip random nanolasers where the cavity feedback is provided by the intrinsic disorder. The strong confinement achieved by Anderson localization reduces the spatial overlap between lasing modes...

  17. Privatised Keynesianism: An Unacknowledged Policy Regime

    Crouch, C.

    2009-01-01

    There have now been two successive policy regimes since the Second World War that have temporarily succeeded in reconciling the uncertainties and instabilities of a capitalist economy with democracy’s need for stability for people’s lives and capitalism’s own need for confident mass consumers. The first of these was the system of public demand management generally known as Keynesianism. The second was not, as has often been thought, a neo-liberal turn to pure markets, but a system of markets ...

  18. Asymptotic Regime in N Random Interacting Species

    Fiasconaro, A; Valenti, D

    2005-01-01

    The asymptotic regime of a complex ecosystem with N random interacting species and in the presence of an external multiplicative noise is analyzed. We find the role of the external noise on the long time probability distribution of the i_th density species, the extinction of species and the local field acting on the i_th population. We analyze in detail the transient dynamics of this field and the cavity field, which is the field acting on the i_th species when this is absent. We find that the presence or the absence of some population give different asymptotic distributions of these fields.

  19. Reconfigurable photoinduced metamaterials in the microwave regime

    Rizza, Carlo; De Paulis, Francesco; Palange, Elia; Orlandi, Antonio; Columbo, Lorenzo; Prati, Franco

    2014-01-01

    We investigate optically reconfigurable dielectric metamaterials at gigahertz frequencies. More precisely, we study the microwave response of a subwavelength grating optically imprinted into a semiconductor slab. In the homogenized regime, we analytically evaluate the ordinary and extraordinary component of the effective permittivity tensor by taking into account the photo-carrier dynamics described by the ambipolar diffusion equation. We analyze the impact of semiconductor parameters on the gigahertz metamaterial response which turns out to be highly reconfigurable by varying the photogenerated grating and which can show a marked anisotropic behavior.

  20. Asian Aspirations for Climate Regime Beyond 2012

    This report is based on the second round of 'The Asia-Pacific Consultations on Climate Regime Beyond 2012' in 2006 to further advance the frank exchange of opinions of policy makers, academics, and the private sector on specific issues of high priority to the region. These include: energy security and development, Clean Development Mechanism (CDM), technology development and transfer, and adaptation. This report presents a summary of what has been learnt through the current consultations, interviews, and questionnaire surveys with policy makers and climate policy researchers across the region

  1. Regulatory Regimes and Takeovers of U.S. Thrifts

    Fatma Cebenoyan; A. Sinan Cebenoyan; Elizabeth S. Cooperman

    2003-01-01

    This paper examines the effect of regulatory regime changes on the attributes of acquired thrifts for periods of stringency in 1990 to 1993, and deregulation in 1994 to 2000, with the removal of significant impediments for bank takeovers of thrifts. We test a regime change hypothesis that predicts a more effective takeover market in the later regime. Consistent with the hypothesis, we find bank acquirers to engage in diverse motivations for takeovers in the later regime, including revenue tur...

  2. The design of environmental regimes: Social construction, contextuality, and improvisation

    Lejano

    2006-01-01

    While much of the literature on environmental regimes has focused on effectiveness, this article takes a new look at a lesser-studied topic, the evolution of regime design. Understanding how regimes differ in design, and how various factors and processes shape such design, is important if we are to more carefully craft these regimes. We should also pay close attention to the formative role of social construction and context. Focusing on transboundary marine programs, we see that their designs...

  3. The role of the media in establishing international security regimes

    Chanan Naveh

    2005-01-01

    This article focuses primarily on the media's impact on international security regimes. It explores the ways in which the media affect the lifecycles of international regimes, from the time they are first conceived of, through their establishment, consolidation and stabilization, up until their ultimate demise. Although this paper highlights the media's role in the evolution of security regimes, it is clear that, regardless of the regime in question, media play a role throughout the lifecycle...

  4. Low-frequency regime transitions and predictability of regimes in a barotropic model

    Nadiga, B T

    2016-01-01

    Predictability of flow is examined in a barotropic vorticity model that admits low frequency regime transitions between zonal and dipolar states. Such transitions in the model were first studied by Bouchet and Simonnet (2009) and are reminiscent of regime change phenomena in the weather and climate systems wherein extreme and abrupt qualitative changes occur, seemingly randomly, after long periods of apparent stability. Mechanisms underlying regime transitions in the model are not well understood yet. From the point of view of atmospheric and oceanic dynamics, a novel aspect of the model is the lack of any source of background gradient of potential-vorticity such as topography or planetary gradient of rotation rate (e.g., as in Charney & DeVore '79). We consider perturbations that are embedded onto the system's chaotic attractor under the full nonlinear dynamics as bred vectors---nonlinear generalizations of the leading (backward) Lyapunov vector. We find that ensemble predictions that use bred vector per...

  5. The trade regime and the climate regime. Institutional evolution and adaptation

    This article addresses concerns that the multilateral trade regime centered in the WTO and the emerging climate regime may conflict in ways that could be damaging to either or both. The article discusses the institutional and diplomatic context of these concerns, and it identifies the kinds of issues that are in question. The analysis suggests that there are opportunities for win-win outcomes in the interactions of the two regimes, for instance in the possibility of reducing fossil fuel subsidies. However, there are also problematic areas where they intersect. A core issue-and as yet an unresolved one-is whether and how emission credit trading and other activities envisioned by the Kyoto Protocol would be subject to WTO rules. The resolution of this issue will affect many other issues as well. Additional specific issues about the interactions of particular provisions in WTO agreements and the Kyoto Protocol are analyzed in a subsequent companion article in Climate Policy

  6. Level-ARCH Short Rate Models with Regime Switching

    Christiansen, Charlotte

    This paper introduces regime switching volatility into level- ARCH models for the short rates of the US, the UK, and Germany. Once regime switching and level effects are included there are no gains from including ARCH effects. It is of secondary importance exactly how the regime switching is spec...

  7. Current status and key issues of nuclear liability regime

    Nuclear liability regime has been established from the beginning of the nuclear power industry. The first nuclear accident with major transboundary consequences occurred at Chernobyl, and led to reexamination of the nuclear liability regime. Current status and key issues of the national and international nuclear liability regime are reviewed. (author)

  8. The global nuclear safety regime and its impact in Brazil

    This work describes the Global Nuclear Safety Regime that was established worldwide after the accident at the Tchernobyl nuclear power plant. This regime is composed of biding international safety conventions, globally accepted safety standard, and a voluntary peer review system. The impact of this Global Regime in Brazil is also discussed. (Author)

  9. Change of secondary water regime of Paks NPP. Change of secondary water regime at Unit 2

    The installation of high pH water regime during the 17th cycle of Unit 2 aimed to decrease the amount of transportation inlet of erosion-corrosion products (magnitude) in feedwater to SGs. The resolution of OAH-NBI permitting the installation ordained to make an evaluation of the process. The main conclusions and results are discussed. The high pH water regime proved to be adequate in the case of Unit 2 as well, similarly to Units 3, 4 and 1. (R.P.)

  10. Strengthening the non proliferation regime: French views

    3 main issues can be identified in the French policy concerning the backing of non proliferation: 1) responding resolutely to proliferation crises, 2) reinforcing substantive efforts to prevent and impede proliferation, and 3) strengthening the non-proliferation regime. The first issue is very important because combating proliferation is vital to the security of all. Concerning the second issue, France attaches particular importance to strengthening specific measures to prevent and check proliferation. Let me mention a few proposals that we put forward: exports need to be controlled more effectively, proliferation activities have to be criminalized, or the development of proliferation-resistant technologies should be supported. Concerning the third issue it means the strengthening of the non-proliferation regime, France proposes several means: -) aiming at the universalization of the additional protocol; -) ensuring that the Agency continues to have sufficient human, financial and technical resources to fulfill its verification mission effectively; -) encouraging the IAEA to make full use of the authority available to it; -) enhancing the use of information relevant to the delivery of the IAEA mandate; and -) sharing more accurate information concerning the breaches of commitments that happen. The paper is followed by the slides of the presentation. (A.C.)

  11. Dynamic regimes of random fuzzy logic networks

    Random multistate networks, generalizations of the Boolean Kauffman networks, are generic models for complex systems of interacting agents. Depending on their mean connectivity, these networks exhibit ordered as well as chaotic behavior with a critical boundary separating both regimes. Typically, the nodes of these networks are assigned single discrete states. Here, we describe nodes by fuzzy numbers, i.e. vectors of degree-of-membership (DOM) functions specifying the degree to which the nodes are in each of their discrete states. This allows our models to deal with imprecision and uncertainties. Compatible update rules are constructed by expressing the update rules of the multistate network in terms of Boolean operators and generalizing them to fuzzy logic (FL) operators. The standard choice for these generalizations is the Goedel FL, where AND and OR are replaced by the minimum and maximum of two DOMs, respectively. In mean-field approximations we are able to analytically describe the percolation and asymptotic distribution of DOMs in random Goedel FL networks. This allows us to characterize the different dynamic regimes of random multistate networks in terms of FL. In a low-dimensional example, we provide explicit computations and validate our mean-field results by showing that they agree well with network simulations.

  12. Regimes of piezoelectric transformer operation II

    Piezoelectric transformers (PTs) have some attractive features for using them in power supply systems such as high efficiency and high power density, a high level of isolation between primary and secondary sides, a flat design which is well suited for a lot of applications and a low level of electromagnetic noise, just to name a few. For any application it is of utmost importance to know the behavior of a piezoelectric transformer under different conditions, which we call regimes of transformer operation. Based on this knowledge, criteria for safe operation of the PT in the power supply system and hence for the whole system as well can be derived. After having introduced the regimes of piezoelectric transformer operation in a first article on this topic in 2006 [1], in this paper we will present the derivation of important parameters based on measurements by way of an example of a symmetric PT. These measurements facilitate the basic understanding for choosing the appropriate transformer parameters necessary for its optimal as well as safe and controlled operation in power supply systems

  13. Elastic regimes of subisostatic athermal fiber networks

    Licup, A. J.; Sharma, A.; MacKintosh, F. C.

    2016-01-01

    Athermal models of disordered fibrous networks are highly useful for studying the mechanics of elastic networks composed of stiff biopolymers. The underlying network architecture is a key aspect that can affect the elastic properties of these systems, which include rich linear and nonlinear elasticity. Existing computational approaches have focused on both lattice-based and off-lattice networks obtained from the random placement of rods. It is not obvious, a priori, whether the two architectures have fundamentally similar or different mechanics. If they are different, it is not clear which of these represents a better model for biological networks. Here, we show that both approaches are essentially equivalent for the same network connectivity, provided the networks are subisostatic with respect to central force interactions. Moreover, for a given subisostatic connectivity, we even find that lattice-based networks in both two and three dimensions exhibit nearly identical nonlinear elastic response. We provide a description of the linear mechanics for both architectures in terms of a scaling function. We also show that the nonlinear regime is dominated by fiber bending and that stiffening originates from the stabilization of subisostatic networks by stress. We propose a generalized relation for this regime in terms of the self-generated normal stresses that develop under deformation. Different network architectures have different susceptibilities to the normal stress but essentially exhibit the same nonlinear mechanics. Such a stiffening mechanism has been shown to successfully capture the nonlinear mechanics of collagen networks.

  14. Dynamic regimes of random fuzzy logic networks

    Wittmann, Dominik M.; Theis, Fabian J.

    2011-01-01

    Random multistate networks, generalizations of the Boolean Kauffman networks, are generic models for complex systems of interacting agents. Depending on their mean connectivity, these networks exhibit ordered as well as chaotic behavior with a critical boundary separating both regimes. Typically, the nodes of these networks are assigned single discrete states. Here, we describe nodes by fuzzy numbers, i.e. vectors of degree-of-membership (DOM) functions specifying the degree to which the nodes are in each of their discrete states. This allows our models to deal with imprecision and uncertainties. Compatible update rules are constructed by expressing the update rules of the multistate network in terms of Boolean operators and generalizing them to fuzzy logic (FL) operators. The standard choice for these generalizations is the Gödel FL, where AND and OR are replaced by the minimum and maximum of two DOMs, respectively. In mean-field approximations we are able to analytically describe the percolation and asymptotic distribution of DOMs in random Gödel FL networks. This allows us to characterize the different dynamic regimes of random multistate networks in terms of FL. In a low-dimensional example, we provide explicit computations and validate our mean-field results by showing that they agree well with network simulations.

  15. Three novel tokamak plasma regimes in TFTR

    Aside from extending ''standard'' ohmic and neutral beam heating studies to advanced plasma parameters, TFTR has encountered a number of special plasma regimes that have the potential to shed new light on the physics of tokamak confinement and the optimal design of future D-T facilities: (1) High-powered, neutral beam heating at low plasma densities can maintain a highly reactive hot-ion population (with quasi-steady-state beam fueling and current drive) in a tokamak configuration of modest bulk-plasma confinement requirements. (2) Plasma displacement away from limiter contact lends itself to clarification of the role of edge-plasma recycling and radiation cooling within the overall pattern of tokamak heat flow. (3) Noncentral auxiliary heating (with a ''hollow'' power-deposition profile) should serve to raise the central tokamak plasma temperature without deterioration of central region confinement, thus facilitating the study of alpha-heating effects in TFTR. The experimental results of regime (3) support the theory that tokamak profile consistency is related to resistive kink stability and that the global energy confinement time is determined by transport properties of the plasma edge region

  16. Three novel tokamak plasma regimes in TFTR

    Furth, H.P.

    1985-10-01

    Aside from extending ''standard'' ohmic and neutral beam heating studies to advanced plasma parameters, TFTR has encountered a number of special plasma regimes that have the potential to shed new light on the physics of tokamak confinement and the optimal design of future D-T facilities: (1) High-powered, neutral beam heating at low plasma densities can maintain a highly reactive hot-ion population (with quasi-steady-state beam fueling and current drive) in a tokamak configuration of modest bulk-plasma confinement requirements. (2) Plasma displacement away from limiter contact lends itself to clarification of the role of edge-plasma recycling and radiation cooling within the overall pattern of tokamak heat flow. (3) Noncentral auxiliary heating (with a ''hollow'' power-deposition profile) should serve to raise the central tokamak plasma temperature without deterioration of central region confinement, thus facilitating the study of alpha-heating effects in TFTR. The experimental results of regime (3) support the theory that tokamak profile consistency is related to resistive kink stability and that the global energy confinement time is determined by transport properties of the plasma edge region.

  17. Tlatelolco regime and nonproliferation in Latin America

    The regime established by the Treaty of Tlatelolco supports peace and security in the Latin American region and global nonproliferation efforts. Circumstances leading to the creation of the nuclear-weapon-free zone include careful preparations and negotiations, individual leadership, existence of certain shared cultural and legal traditions of Latin American countries, and the temporary stimulus of the Cuban missile crisis. The lack of overt superpower pressure on Latin America, compared with more turbulent regions, has permitted continued progress toward full realization of the zone. Tlatelolco's negotiating process, as well as the substance of the Treaty, deserve careful consideration relative to other areas. The Treaty enjoys wide international approval, but full support by certain Latin American States (Argentina, Brazil, Chile, Cuba) has been negatively affected by the failure of the US Senate to ratify Tlatelolco's Protocol I. Nuclear programs of Argentina, Brazil, and Mexico are expanding rapidly and these nations are forming linkages with West European countries, rather than the United States. The May 1980 Argentine-Brazilian nuclear agreement foresees significant cooperation between the two nation's nuclear energy commissions and more coordinated resistance to the nuclear supplier countries. Argentine-Brazilian nuclear convergence and the response accorded to it by the United States will have significant implications for the future of the Tlatelolco regime and nonproliferation in Latin America. 52 references

  18. Elastic regimes of subisostatic athermal fiber networks.

    Licup, A J; Sharma, A; MacKintosh, F C

    2016-01-01

    Athermal models of disordered fibrous networks are highly useful for studying the mechanics of elastic networks composed of stiff biopolymers. The underlying network architecture is a key aspect that can affect the elastic properties of these systems, which include rich linear and nonlinear elasticity. Existing computational approaches have focused on both lattice-based and off-lattice networks obtained from the random placement of rods. It is not obvious, a priori, whether the two architectures have fundamentally similar or different mechanics. If they are different, it is not clear which of these represents a better model for biological networks. Here, we show that both approaches are essentially equivalent for the same network connectivity, provided the networks are subisostatic with respect to central force interactions. Moreover, for a given subisostatic connectivity, we even find that lattice-based networks in both two and three dimensions exhibit nearly identical nonlinear elastic response. We provide a description of the linear mechanics for both architectures in terms of a scaling function. We also show that the nonlinear regime is dominated by fiber bending and that stiffening originates from the stabilization of subisostatic networks by stress. We propose a generalized relation for this regime in terms of the self-generated normal stresses that develop under deformation. Different network architectures have different susceptibilities to the normal stress but essentially exhibit the same nonlinear mechanics. Such a stiffening mechanism has been shown to successfully capture the nonlinear mechanics of collagen networks. PMID:26871101

  19. Flow regime identification methodology with computerized tomography

    This work presents a flow identification methodology in multiphase system in stratified oil-water-gas regime. The principle is based on detecting the amount of x-rays attenuated by passing through the duct containing the flow. The radiation interacts with the material and the composite duct fluid, causing the intensity of X ray declines. In this study, was used divergent bean into two and three dimensions. In this case we need to know the source position in order to back project the rays accordingly in a divergent way. In three dimensions, the projections discontinuity determination problem is substituted by the projections images contour determination. Each ray generates one algebraic equation. The system may be solved by the least square method or by some algebraic reconstruction technique such as the ART, MART or q-ART algorithms. The experiment shows that with a single view (a source-detector pair) it is possible to obtain information of a flow inside a duct (the attenuation of the radiation beam). However, it is not possible to make the reconstruction of the flow. Then, we use two views, so getting enough information to reconstruct the flow. The results presented show that the technique may be applied in the successfully flow regime identification. (author)

  20. Flow regime identification methodology with computerized tomography

    Teixeira, Alberto Ramon Ferreira; Roberty, Nilson Costa, E-mail: ateixeira@con.ufrj.b, E-mail: nilson@nuclear.ufrj.b [Universidade Federal do Rio de Janeiro (COPPE/UFRJ), RJ (Brazil). Nuclear Engineering Program

    2010-07-01

    This work presents a flow identification methodology in multiphase system in stratified oil-water-gas regime. The principle is based on detecting the amount of x-rays attenuated by passing through the duct containing the flow. The radiation interacts with the material and the composite duct fluid, causing the intensity of X ray declines. In this study, was used divergent bean into two and three dimensions. In this case we need to know the source position in order to back project the rays accordingly in a divergent way. In three dimensions, the projections discontinuity determination problem is substituted by the projections images contour determination. Each ray generates one algebraic equation. The system may be solved by the least square method or by some algebraic reconstruction technique such as the ART, MART or q-ART algorithms. The experiment shows that with a single view (a source-detector pair) it is possible to obtain information of a flow inside a duct (the attenuation of the radiation beam). However, it is not possible to make the reconstruction of the flow. Then, we use two views, so getting enough information to reconstruct the flow. The results presented show that the technique may be applied in the successfully flow regime identification. (author)

  1. Essence and Prospect of International Export Control Regimes

    2005-01-01

    @@ International export control regimes are referring to the regimes and systems concerning limitation of export and transference of military-use and dual-use items and related parts as well as technologies. In the past couple of years, the control regimes have shown a strengthening posture. In addition, a new global export control regime is brewing. Hence export control has caught the attention of the international community, and particularly of the circles of armament control and commerce. The author would like to make some analyses of the posture and then try to predict the trend of international export control regimes.

  2. Quaternary Faults and Stress Regime of Venezuela

    F.A. Audemard M.

    2006-12-01

    Full Text Available Spatial configuration of Quaternary active tectonic features along the southern Caribbean plate boundary suggests that the region is subject to a compressive strike-slip (transpressional senso lato regime, characterized by a NNW-SSE maximum horizontal stress (sH=s1 and/or an ENE-WSW minimum (s h=s3 or s2 horizontal stress. Stress inversion applied to fault-plane kinematic indicators measured essentially in Plio-Quaternary sedimentary rocks confirms this tectonic regime. Accordingly, this stress regime is responsible for the Quaternary activity and kinematics of six sets of brittle features along northern Venezuela (from Colombia in the west to Trinidad in the east: (1 east-west rightlateral faults, (2 NW right-lateral faults -acting as synthetic Riedel shears-, (3 ENE to east-west dextral faults -P shears-, (4 NNW normal faults, (5 almost north-south left-lateral faults -antithetic Riedel shears- and (6 mostly subsurface ENE reverse faults associated with folding of the same orientation. Brittle deformation conforms to the simple shear model, although not all the deformation can be accounted for it since strain partitioning is also taking place because regional folding and thrusting are due to the normal-to-structure component of the relative slip vector between the Caribbean and South America plates. On the other hand, the maximum horizontal stress in western Venezuela, particularly in the Maracaibo block and south of the Oca-Ancón fault, progressively turns counter-clockwise to become more east-west oriented, producing left- and right-lateral slip along the north-south striking and NE-SW striking faults, respectively. The orientation and spatial variation of this regional stress field in western Venezuela results from the superposition of the two major neighboring interplate maximum horizontal stress orientations (sH: roughly east-west trending stress across the Nazca-South America type-B subduction along the pacific coast of Colombia and NNW

  3. Progress towards a global nuclear liability regime

    As the production and use of nuclear energy for peaceful purposes developed in the 1950s, a specific legal framework for third party nuclear liability was established to ensure adequate compensation for damage to persons and property resulting from a nuclear accident, but also to encourage the industry to develop nuclear technology and assume responsibility without being exposed to an uncertain and potentially ruinous liability burden. Significant attention has been understandably placed at the international and national levels on fostering strong programmes to achieve safety, security and safeguards at a high level. Notwithstanding best efforts to achieve a high level of safety, the possibility remains that accidents may occur within a nuclear installation (i.e. not only nuclear power plants but also any installation in which there are nuclear fuel, nuclear substances, radioactive products or waste) or during the transportation of nuclear substances to or from a nuclear installation. As the experience shows from the accidents that occurred at Three Mile Island (United States) in 1979, Chernobyl (former USSR) in 1986, and Fukushima Daiichi (Japan) in 2011, severe accidents can have varying and potentially far-reaching consequences affecting both people and property. In the wake of the Fukushima Daiichi nuclear power plant accident, the General Conference of the International Atomic Energy Agency (IAEA) endorsed in September 2011 an Action Plan on Nuclear Safety (“IAEA Action Plan”) to strengthen the global nuclear safety framework. The IAEA Action Plan calls upon member states “to work towards establishing a global nuclear liability regime that addresses the concerns of all states that might be affected by a nuclear accident with a view to providing appropriate compensation for nuclear damage”, and “to give due consideration to the possibility of joining the international nuclear liability instruments as a step towards achieving such a global regime

  4. No interactions between previously associated 2-hour glucose gene variants and physical activity or BMI on 2-hour glucose levels

    Scott, Robert A; Chu, Audrey Y; Grarup, Niels;

    2012-01-01

    Gene-lifestyle interactions have been suggested to contribute to the development of type 2 diabetes. Glucose levels 2 h after a standard 75-g glucose challenge are used to diagnose diabetes and are associated with both genetic and lifestyle factors. However, whether these factors interact...... to determine 2-h glucose levels is unknown. We meta-analyzed single nucleotide polymorphism (SNP) × BMI and SNP × physical activity (PA) interaction regression models for five SNPs previously associated with 2-h glucose levels from up to 22 studies comprising 54,884 individuals without diabetes. PA levels were...

  5. Institutional design and regime effectiveness in transboundary river management – the Elbe water quality regime

    I. Dombrowsky

    2007-06-01

    Full Text Available The literature on transboundary river management suggests that institutions play an important role in bringing cooperation about. However, the knowledge on how they should be designed in order to do so remains limited. One way to learn more about adequate institutional design is to assess the effectiveness of existing regimes, and to trace the causal relationships leading to the respective outcomes. In order to gain further insights into the relationship of institutional design and regime effectiveness, this paper presents a study on the water quality regime of the International Commission for the Protection of the Elbe (ICPE. The analysis is based on a review of pertinent documents and ten qualitative interviews with Czech and German Commission members and NGO representatives. Particular emphasis has been put on determining the ICPE's specific contribution and the no-regime counterfactual as well as on the perceived expedience of the institutional arrangements. The study shows that overall due to external as well as internal institutional factors the ICPE proved relatively successful, and as such it also provides insights into how institutions matter: The commission served as platform for joint problem solving by identifying priorities for action. These international obligations increased the power of national administrations and their access to funds. At the same time, the Commission's reporting to the public served as an enforcement mechanism. However, the ICPE's contribution towards achieving the various goals varied significantly between the different areas of activity. It was high where the main responsibility for action was with the public authorities, such as in the area of wastewater treatment and the establishment of an international alarm plan and model. It was practically non existent in the reduction of non-point pollution from agriculture, where the success depended on the behavior of individual private actors (farmers. From a

  6. Regime shifts in models of dryland vegetation

    Zelnik, Yuval R; Yizhaq, Hezi; Bel, Golan; Meron, Ehud

    2013-01-01

    Drylands are pattern-forming systems showing self-organized vegetation patchiness, multiplicity of stable states and fronts separating domains of alternative stable states. Pattern dynamics, induced by droughts or disturbances, can result in desertification shifts from patterned vegetation to bare soil. Pattern-formation theory suggests various scenarios for such dynamics; an abrupt global shift involving a fast collapse to bare soil, a gradual global shift involving the expansion and coalescence of bare-soil domains, and an incipient shift to a hybrid state consisting of stationary bare-soil domains in an otherwise periodic pattern. Using models of dryland vegetation we address the question which of these scenarios can be realized. We found that the models can be split into two groups: models that exhibit multiplicity of periodic-pattern and bare-soil states, and models that exhibit, in addition, multiplicity of hybrid states. Furthermore, in all models we could not identify parameter regimes in which bare-s...

  7. Two regimes of forced turbulent convection

    Eidelman, A; Kleeorin, N; Rogachevskii, I; Sapir-Katiraie, I

    2009-01-01

    We study experimentally a forced turbulent convection in the Rayleigh-B\\'{e}nard apparatus with an additional source of turbulence produced by the two oscillating grids located nearby the side walls of the chamber. Two different regimes have been observed in the forced turbulent convection. When the frequency of the grid oscillations f>2 Hz, the large-scale circulation (LSC) is totally destroyed, and the destruction of the LSC is accompanied by a strong change of the mean temperature distribution. For the very low frequency the thermal structure inside the LSC is inhomogeneous and anisotropic. The hot thermal plumes accumulate at one side of LSC, and cold plumes concentrate at the opposite side of LSC. The mean temperature gradient in the horizontal direction inside the LSC is significantly larger than in the vertical direction. For the high frequency (f > 10 Hz), LSC has not been observed and the mean temperature gradient in the central flow region in the vertical direction, \

  8. The Great Lakes' regional climate regimes

    Sugiyama, Noriyuki

    For the last couple of decades, the Great Lakes have undergone rapid surface warming. In particular, the magnitude of the summer surface-warming trends of the Great Lakes have been much greater than those of surrounding land (Austin and Colman, 2007). Among the Great Lakes, the deepest Lake Superior exhibited the strongest warming trend in its annual, as well as summer surface water temperature. We find that many aspects of this behavior can be explained in terms of the tendency of deep lakes to exhibit multiple regimes characterized, under the same seasonally varying forcing, by the warmer and colder seasonal cycles exhibiting different amounts of wintertime lake-ice cover and corresponding changes in the summertime lake-surface temperatures. In this thesis, we address the problem of the Great Lakes' warming using one-dimensional lake modeling to interpret diverse observations of the recent lake behavior. (Abstract shortened by ProQuest.).

  9. Environment Flow Assessment with Flow Regime Transition

    Su, J.; Ho, C. C.; Chang, L. C.

    2015-12-01

    To avoid worsen river and estuarine ecosystems cause by overusing water resources, environmental flows conservation is applied to reduce the impact of river environment. Environmental flows refer to water provided within a river, wetland or coastal zone to sustain ecosystems and benefits to human wellbeing. Environment flow assessment is now widely accepted that a naturally variable flow regime, rather than just a minimum low flow. In this study, we propose four methods, experience method, Tenant method, hydraulic method and habitat method to assess the environmental flow of base flow, flush flow and overbank flow with different discharge, frequency and occurrence period. Dahan River has been chosen as a case to demonstrate the assessment mechanism. The alternatives impact analysis of environment and human water used provides a reference for stakeholders when holding an environmental flow consultative meeting.

  10. Deterministic-random separation in nonstationary regime

    Abboud, D.; Antoni, J.; Sieg-Zieba, S.; Eltabach, M.

    2016-02-01

    In rotating machinery vibration analysis, the synchronous average is perhaps the most widely used technique for extracting periodic components. Periodic components are typically related to gear vibrations, misalignments, unbalances, blade rotations, reciprocating forces, etc. Their separation from other random components is essential in vibration-based diagnosis in order to discriminate useful information from masking noise. However, synchronous averaging theoretically requires the machine to operate under stationary regime (i.e. the related vibration signals are cyclostationary) and is otherwise jeopardized by the presence of amplitude and phase modulations. A first object of this paper is to investigate the nature of the nonstationarity induced by the response of a linear time-invariant system subjected to speed varying excitation. For this purpose, the concept of a cyclo-non-stationary signal is introduced, which extends the class of cyclostationary signals to speed-varying regimes. Next, a "generalized synchronous average'' is designed to extract the deterministic part of a cyclo-non-stationary vibration signal-i.e. the analog of the periodic part of a cyclostationary signal. Two estimators of the GSA have been proposed. The first one returns the synchronous average of the signal at predefined discrete operating speeds. A brief statistical study of it is performed, aiming to provide the user with confidence intervals that reflect the "quality" of the estimator according to the SNR and the estimated speed. The second estimator returns a smoothed version of the former by enforcing continuity over the speed axis. It helps to reconstruct the deterministic component by tracking a specific trajectory dictated by the speed profile (assumed to be known a priori).The proposed method is validated first on synthetic signals and then on actual industrial signals. The usefulness of the approach is demonstrated on envelope-based diagnosis of bearings in variable

  11. The role of the media in establishing international security regimes

    Chanan Naveh

    2005-04-01

    Full Text Available This article focuses primarily on the media's impact on international security regimes. It explores the ways in which the media affect the lifecycles of international regimes, from the time they are first conceived of, through their establishment, consolidation and stabilization, up until their ultimate demise. Although this paper highlights the media's role in the evolution of security regimes, it is clear that, regardless of the regime in question, media play a role throughout the lifecycle of all international regimes, whatever their character. In order to analyze the relationships of the media with security regimes in general, and specifically their contribution to each stage in their development, the article utilizes methodologies from the field of communication studies. It examines the media's agenda, "news values" and various functions, and their ability to mobilize public support for the particular issue of the regime. To date, most studies have explored the interaction between media communication patterns and global developments at the state level, or in relation to the formulation of foreign policy, while largely ignoring the international dimension of the relationship. This article attempts to remedy this situation, and the relevant processes are analysed in a case study of the anti-Iraq international security regime. It should be noted that although the paper focuses on the specific anti-Iraqi regime, it is part of a more general Anti-Rogue actors regime which includes the war against global terrorism. The study of the development of the anti-Iraq press-security regime teaches us that during international crises the media mobilize and unanimously support the regime fighting the "bad guys." But, when the regime develops and enters disputed turfs and begins to lose its legitimacy, media support diminishes, and the media may even develop into an opposing force and may join the actors fighting against this regime. Moreover, the study of

  12. Spacetime-noncommutativity regime of Loop Quantum Gravity

    AMELINO-CAMELIA, Giovanni; da Silva, Malú Maira; Ronco, Michele; Cesarini, Lorenzo; Lecian, Orchidea Maria

    2016-01-01

    A recent study by Bojowald and Paily provided a path toward the identification of an effective quantum-spacetime picture of Loop Quantum Gravity, applicable in the "Minkowski regime", the regime where the large-scale (coarse-grained) spacetime metric is flat. A pivotal role in the analysis is played by Loop-Quantum-Gravity-based modifications to the hypersurface deformation algebra, which leave a trace in the Minkowski regime. We here show that the symmetry-algebra results reported by Bojowal...

  13. Apparel exportism in Kenya: international regimes, chain governance and upgrading

    Kindiki, M. M.

    2011-01-01

    An important question in development is how far can the contemporary global context create industrial development opportunities for the South, particularly for Low Income Countries? In an important sense, this can only be answered in non-abstract terms, since the institutional conditions facing particular industries at particular times are highly specific. In this research, a configuration of four regimes- the structural regime on production and trade in apparel, the labour regime, the neo-st...

  14. Exchange Rate Regime Analysis Using Structural Change Methods

    Zeileis, Achim; Shah, Ajay; Patnaik, Ila

    2007-01-01

    Regression models for de facto currency regime classification are complemented by inferential techniques for tracking the stability of exchange rate regimes. Several structural change methods are adapted to these regressions: tools for assessing the stability of exchange rate regressions in historical data (testing), in incoming data (monitoring) and for determining the breakpoints of shifts in the exchange rate regime (dating). The tools are illustrated by investigating the Chinese exchange ...

  15. Fixed or Floating Exchange Regimes; Does it Matter for Inflation?

    Peter J. Quirk

    1994-01-01

    This paper reviews recent experience with the choice of floating or fixed (“anchor”) exchange regimes in industrial and developing countries. It concludes that desirable differences between the two sets of regimes have narrowed, owing to the useful operational role of exchange rate margins and unavoidable medium-term rate adjustments in the context of fixed regimes. A survey of recent empirical cross-country literature also suggests little unambiguous association of the choice of exchange reg...

  16. Detection and Assessment of Ecosystem Regime Shifts from Fisher Information

    B. Roy Frieden; Christopher W. Pawlowski; Heriberto Cabezas; Arunprakash T. Karunanithi

    2008-01-01

    Ecosystem regime shifts, which are long-term system reorganizations, have profound implications for sustainability. There is a great need for indicators of regime shifts, particularly methods that are applicable to data from real systems. We have developed a form of Fisher information that measures dynamic order in complex systems. Here we propose the use of Fisher information as a means of: (1) detecting dynamic regime shifts in ecosystems, and (2) assessing the quality of the shift in terms...

  17. Scaling regimes in spherical shell rotating convection

    Gastine, T; Aubert, J

    2016-01-01

    Rayleigh-B\\'enard convection in rotating spherical shells can be considered as a simplified analogue of many astrophysical and geophysical fluid flows. Here, we use three-dimensional direct numerical simulations to study this physical process. We construct a dataset of more than 200 numerical models that cover a broad parameter range with Ekman numbers spanning $3\\times 10^{-7} \\leq E \\leq 10^{-1}$, Rayleigh numbers within the range $10^3 < Ra < 2\\times 10^{10}$ and a Prandtl number unity. We investigate the scaling behaviours of both local (length scales, boundary layers) and global (Nusselt and Reynolds numbers) properties across various physical regimes from onset of rotating convection to weakly-rotating convection. Close to critical, the convective flow is dominated by a triple force balance between viscosity, Coriolis force and buoyancy. For larger supercriticalities, a subset of our numerical data approaches the asymptotic diffusivity-free scaling of rotating convection $Nu\\sim Ra^{3/2}E^{2}$ in ...

  18. On the ontological emergence from quantum regime

    Luty, Damian [Adam Mickiewicz University, Poznan (Poland)

    2014-07-01

    There are several views on the relation between quantum physics and theory of relativity (especially General Relativity, GR). A popular perspective is this: GR with its macroscopic gravitational effects will turn out to be a limit of a more fundamental theory which should consider discrete physics and not deal with continuity (like theory of relativity). Thus, GR will emerge from a more basic theory, which should be quantum-like. One could call this an epistemic emergence view towards fundamental theories. The question is, given that scientific realism is valid: should emergence be a fundamental notion in our ontological view about the evolving, physical Universe? Is there an ontological emergence fully compatible with the notion of fundamentality? I argue that if we want to defend ontological emergence (from quantum to macroscopic regime) as something fundamental, we will arrive at the position of metaphysics of dispositions (and I argue, why this is undesirable), or conclude, that we cannot square fully fundamental ontology with the notion of emergence, and that we have to accept an ontological pluralism relativised to a certain scale. I defend the latter proposition, showing, that epistemic emergence doesn't entail (logically) ontological emergence.

  19. On the ontological emergence from quantum regime

    There are several views on the relation between quantum physics and theory of relativity (especially General Relativity, GR). A popular perspective is this: GR with its macroscopic gravitational effects will turn out to be a limit of a more fundamental theory which should consider discrete physics and not deal with continuity (like theory of relativity). Thus, GR will emerge from a more basic theory, which should be quantum-like. One could call this an epistemic emergence view towards fundamental theories. The question is, given that scientific realism is valid: should emergence be a fundamental notion in our ontological view about the evolving, physical Universe? Is there an ontological emergence fully compatible with the notion of fundamentality? I argue that if we want to defend ontological emergence (from quantum to macroscopic regime) as something fundamental, we will arrive at the position of metaphysics of dispositions (and I argue, why this is undesirable), or conclude, that we cannot square fully fundamental ontology with the notion of emergence, and that we have to accept an ontological pluralism relativised to a certain scale. I defend the latter proposition, showing, that epistemic emergence doesn't entail (logically) ontological emergence.

  20. Revamp urged for Norway's offshore regime

    This paper reports that the Norwegian government has come under renewed pressure from offshore operators to fine tune its licensing procedures and tax regime. The need for change was emphasized at the Advanced Petroleum Conference in Stavanger attended by senior personnel from companies active in the Norwegian and British sectors of the North Sea. Companies have had some success in the tax front, winning changes in proposals from Norway's Ministry of Finance for offshore tax reform. The changes were required to offset a general reduction in the corporate tax to 28% from 50.8%. Norsk Shell has told the government that in view of uncertainty about taxes it will not take part in the normal industry-government discussions about what acreage should be included in the 14th licensing round to be announced next year. This action does not bar Shell from participating in the licensing round when it gets under way. Shell has the if the tax package is not changed sharply in its passage through Storting it is unlikely to bid for new licenses

  1. Multimode optomechanical system in the quantum regime

    Nielsen, William H P; Møller, Christoffer B; Polzik, Eugene S; Schliesser, Albert

    2016-01-01

    We realise a simple and robust optomechanical system with a multitude of long-lived ($Q>10^7$) mechanical modes in a phononic-bandgap shielded membrane resonator. An optical mode of a compact Fabry-Perot resonator detects these modes' motion with a measurement rate ($96~\\mathrm{kHz}$) that exceeds the mechanical decoherence rates already at moderate cryogenic temperatures ($10\\,\\mathrm{K}$). Reaching this quantum regime entails, i.~a., quantum measurement backaction exceeding thermal forces, and thus detectable optomechanical quantum correlations. In particular, we observe ponderomotive squeezing of the output light mediated by a multitude of mechanical resonator modes, with quantum noise suppression up to -2.4 dB (-3.6 dB if corrected for detection losses) and bandwidths $\\lesssim 90\\,\\mathrm{ kHz}$. The multi-mode nature of the employed membrane and Fabry-Perot resonators lends itself to hybrid entanglement schemes involving multiple electromagnetic, mechanical, and spin degrees of freedom.

  2. On spinfoam models in large spin regime

    We study the semiclassical behavior of Lorentzian Engle–Pereira–Rovelli–Livine (EPRL) spinfoam model, by taking into account the sum over spins in the large spin regime. We also employ the method of stationary phase analysis with parameters and the so-called, almost analytic machinery, in order to find the asymptotic behavior of the contributions from all possible large spin configurations in the spinfoam model. The spins contributing the sum are written as Jf = λjf, where λ is a large parameter resulting in an asymptotic expansion via stationary phase approximation. The analysis shows that at least for the simplicial Lorentzian geometries (as spinfoam critical configurations), they contribute the leading order approximation of spinfoam amplitude only when their deficit angles satisfy γ Θ-ring f≤λ−1/2 mod 4πZ. Our analysis results in a curvature expansion of the semiclassical low energy effective action from the spinfoam model, where the UV modifications of Einstein gravity appear as subleading high-curvature corrections. (paper)

  3. Global fishery prospects under contrasting management regimes.

    Costello, Christopher; Ovando, Daniel; Clavelle, Tyler; Strauss, C Kent; Hilborn, Ray; Melnychuk, Michael C; Branch, Trevor A; Gaines, Steven D; Szuwalski, Cody S; Cabral, Reniel B; Rader, Douglas N; Leland, Amanda

    2016-05-01

    Data from 4,713 fisheries worldwide, representing 78% of global reported fish catch, are analyzed to estimate the status, trends, and benefits of alternative approaches to recovering depleted fisheries. For each fishery, we estimate current biological status and forecast the impacts of contrasting management regimes on catch, profit, and biomass of fish in the sea. We estimate unique recovery targets and trajectories for each fishery, calculate the year-by-year effects of alternative recovery approaches, and model how alternative institutional reforms affect recovery outcomes. Current status is highly heterogeneous-the median fishery is in poor health (overfished, with further overfishing occurring), although 32% of fisheries are in good biological, although not necessarily economic, condition. Our business-as-usual scenario projects further divergence and continued collapse for many of the world's fisheries. Applying sound management reforms to global fisheries in our dataset could generate annual increases exceeding 16 million metric tons (MMT) in catch, $53 billion in profit, and 619 MMT in biomass relative to business as usual. We also find that, with appropriate reforms, recovery can happen quickly, with the median fishery taking under 10 y to reach recovery targets. Our results show that commonsense reforms to fishery management would dramatically improve overall fish abundance while increasing food security and profits. PMID:27035953

  4. Regime shifts in a social-ecological system

    Lade, Steven J.; Tavoni, Alessandro; Levin, Simon A.; Schlüter, Maja

    2013-01-01

    Ecological regime shifts are rarely purely ecological. Not only is the regime shift frequently triggered by human activity, but the responses of relevant actors to ecological dynamics are often crucial to the development and even existence of the regime shift. Here, we show that the dynamics of human behaviour in response to ecological changes can be crucial in determining the overall dynamics of the system. We find a social-ecological regime shift in a model of harvesters of a common-pool re...

  5. Institutional design and regime effectiveness in transboundary river management – the Elbe water quality regime

    I. Dombrowsky

    2008-02-01

    Full Text Available The literature on transboundary river management suggests that institutions play an important role in bringing about cooperation. However, knowledge about how such institutions should be designed in order to do so remains limited. One way to learn more about adequate institutional design is to assess the effectiveness of existing regimes, and to trace the causal relationships that lead to the respective outcomes. In order to gain further insights into the relationship between institutional design and regime effectiveness, this paper presents a study on the water quality regime of the International Commission for the Protection of the Elbe (ICPE. The analysis is based on a review of pertinent documents and ten qualitative interviews with Czech and German Commission members and NGO representatives. Particular emphasis has been put on determining the ICPE's specific contribution and the no-regime counterfactual as well as on the perceived expediency of the institutional arrangements. The study shows overall that the countries were relatively successful in improving water quality in the Elbe basin. However, this outcome can only partly be attributed to the ICPE itself. Furthermore, the ICPE's contribution towards achieving the various goals varied significantly between the different areas of activity: it was relatively significant where the main responsibility for action lay with the public authorities, such as in the area of wastewater treatment and the establishment of an international alarm plan and model, but was practically non-existent in the reduction of non-point pollution from agriculture, where success depended on the behavior of individual private actors (farmers. The commission contributed towards problem solving by serving as a forum for the joint identification of priorities for action from a basin-wide perspective. The resulting international obligations increased the power of national water administrations and their access to funds

  6. Varieties of Economic Growth Regimes, Types of Macroeconomic Policies, and Policy Regime: A Post-Keynesian Analysis

    Hiroshi Nishi

    2012-01-01

    This study explores the effects of macroeconomic policies on measures of macroeconomic performance such as growth and inflation by setting up a dynamic post-Keynesian model with government and central bank interventions. In doing so, this study reconsiders the arguments in favor of a policy regime. The model in this paper generates several varieties of economic growth regimes and inflation dynamics. The economic growth regimes are defined by the relationship between economic growth, the incom...

  7. Co-evolution of waste and electricity regimes: Multi-regime dynamics in the Netherlands (1969-2003)

    This article explores how the relation between waste and electricity regimes changed in the Netherlands in a long-term perspective. The concept of socio-technical regime is used to investigate institutional, technological and social (network) changes. The conclusion is that the relationship changed from two regimes being separated into a much more symbiotic and integrated relationship through a multi-level and co-evolutionary process. The concept of 'biomass' has become a binding element in the relationship

  8. MRS2_PLOT: MATLAB function to plot calibration results for a Markov regime-switching (MRS) model with 2 regimes.

    Joanna Janczura; Rafal Weron

    2011-01-01

    MRS2_PLOT(KSI_TT,DATA) generates a figure with two panels visualizing calibration results for a Markov regime-switching (MRS) model with 2 regimes fitted to time series DATA. In the bottom panel probabilities KSI_TT that an observation comes from the spike (i.e. second) regime are plotted. In the top panel the time series is displayed with observations identified as belonging to the spike regime (i.e. having KSI_TT>0.5) marked by red dots. MRS2_PLOT(KSI_TT,DATA,YLAB,XLAB,TIT,POS) allows to sp...

  9. Study on Effect of Shenmai Injection Protecting Myocardium against Ischemia-Reperfusion Injury in Thrombolytic Therapy with Urokinase for Acute Myocardial Infarction Patient Evaluated by 99mTc-MIBI Myocardial Imaging

    2001-01-01

    Objective: To evaluate the myocardial protecting effect of Shenmai injection (SMI) against ischemia/reperfusion injury in thrombolytic therapy with urokinase (UK) for acute myocardial infarction patients by 99mTc-MIBI myocardial imaging (SPECT). Methods: Five hundred and thirty-seven patients were divided into two groups randomly. The SMI group (n=292) was treated with thrombolytictreatment plus SMI and the control group (n=245) with thrombolytic treatment solely. Single photon emission computed tomography (SPECT) was carried out on the 7th day after thrombolysis to determine the ischemic myocardial area (IMA) and ejection fraction (EF) in both groups and compared. Results: The infarction related area (IRA) of reperfusion rate in the two groups was not different significantly (72.26% vs 72.65%, P >0.05). The IMA in patients of the SMI group, no matter with or without reperfused IRA (211 cases and 81 cases) respectively, was significantly lower than that in the control group (178 cases and 67 cases) respectively, P<0.01 and P<0.05 respectively. The EF value in the SMI group was significantly higher than that in the control group (P<0.01). Conclusion:Using SMI in early stage of thrombolytic treatment in acute myocardial infarction could significantly reduce IMA and increase EF. SMI showed good protective effect against myocardial ischemia/reperfusion injury in thrombolytic treatment.

  10. Study on Effect of Shenmai Injection Protecting Myocardium against Ischemia-Reperfusion Injury in Thrombolytic Therapy with Urokinase for Acute Myocardial Infarction Patient Evaluated by 99mTc-MIBI Myocardial Imaging

    郭松鹏; 张言镇

    2001-01-01

    Objective: To evaluate the myocardial protecting effect of Shenmai injection (SMI) against ischemia/reperfusion injury in thrombolytic therapy with urokinase (UK) for acute myocardial infarction patients by 99mTc-MIBI myocardial imaging (SPECT). Methods: Five hundred and thirty-seven patients were divided into two groups randomly. The SMI group (n=292) was treated with thrombolytictreatment plus SMI and the control group (n=245) with thrombolytic treatment solely. Single photon emission computed tomography (SPECT) was carried out on the 7th day after thrombolysis to determine the ischemic myocardial area (IMA) and ejection fraction (EF) in both groups and compared. Results: The infarction related area (IRA) of reperfusion rate in the two groups was not different significantly (72.26% vs 72.65%, P >0.05). The IMA in patients of the SMI group, no matter with or without reperfused IRA (211 cases and 81 cases) respectively, was significantly lower than that in the control group (178 cases and 67 cases) respectively, P<0.01 and P<0.05 respectively. The EF value in the SMI group was significantly higher than that in the control group (P<0.01). Conclusion:Using SMI in early stage of thrombolytic treatment in acute myocardial infarction could significantly reduce IMA and increase EF. SMI showed good protective effect against myocardial ischemia/reperfusion injury in thrombolytic treatment.

  11. The seismology of geothermal regimes. Final report

    Aki, K.

    1997-04-01

    The authors have been developing seismological interpretation theory and methods applicable to complex structures encountered in geothermal areas for a better understanding of the earth`s geothermal regimes. The questions the y have addressed in their research may be summarized as ``What is going on in the earth`s crust under tectonically active regions; what are the structures and processes responsible for such activities as earthquakes and volcanic eruptions; and how can one capture their essence effectively by means of seismological studies?`` First, the authors found clear evidence for localization of scattered seismic energy in the deep magmatic system of the volcano on the island of Reunion in the Indian Ocean. The seismic coda of local earthquakes show concentrated energy in the intrusive zones as late as 30 to 40 seconds after the origin time. This offers a very effective method for defining a zone of strong heterogeneity on a regional scale, complementary to the high resolution study using trapped modes as pursued in the past project. Secondly, the authors identified about 700 long-period events with various frequencies and durations from the data collected during the past 5 years which included three episodes of eruption. They are applying a finite-element method to the simplest event with the longest period and the shortest duration in order to find the location, geometry and physical properties of their source deterministically. The preliminary result described here suggests that their sources may be a horizontally lying magma-filled crack at a shallow depth under the summit area. In addition to the above work on the Reunion data, they have continued the theoretical and observational studies of attenuation and scattering of seismic waves.

  12. Dissipation regimes for short wind waves

    Caulliez, Guillemette

    2013-02-01

    The dissipation processes affecting short wind waves of centimeter and decimeter scales are investigated experimentally in laboratory. The processes include damping due to molecular viscosity, generation of capillary waves, microbreaking, and breaking. The observations were made in a large wind wave tank for a wide range of fetches and winds, using a laser sheet and a high-resolution video camera. The work aims at constructing a comprehensive picture of dissipative processes in the short wind wave field, to find for which scales particular dissipative mechanism may become important. Four distinct regimes have been identified. For capillary-gravity wave fields, i.e., for dominant waves with scales below 4 cm, viscous damping is found to be the main dissipation mechanism. The gravity-capillary wave fields with dominant wavelength less than 10 cm usually exhibit a train of capillary ripples at the crest wavefront, but no wave breaking. For such waves, the main dissipation process is molecular viscosity occurring through nonlinear energy cascade toward high-frequency motions. Microscale breaking takes place for waves longer than 10 cm and manifests itself in a very localized surface disruption on the forward face of the crest. Such events generate turbulent motions in water and thus enhance wave dissipation. Plunging breaking, characterized by formation of a crest bulge, a microjet hitting the water surface and a splash-up, occurs for short gravity waves of wavelength exceeding 20 cm. Macroscale spilling breaking is also observed for longer waves at high winds. In both cases, the direct momentum transfer from breaking waves to the water flow contributes significantly to wave damping.

  13. Antecedents of Teachers Fostering Effort within two different management regimes: An assessment-based accountability regime and regime without external pressure on results

    Knut-Andreas Christophersen; Eyvind Elstad; Are Turmo

    2012-01-01

    This article focuses on the comparison of organizational antecedents of teachers' fostering of students' effort in two quite different accountability regimes: one management regime with an external-accountability system and one with no external accountability devices. The methodology involves cross-sectional surveys from two different management systems: (1) teachers working under assessment-based accountability (N = 236) and (2) folk–high school teachers who work without tests and examinatio...

  14. Antecedents of Teachers Fostering Effort within two different management regimes: An assessment-based accountability regime and regime without external pressure on results

    Knut-Andreas Christophersen

    2012-12-01

    Full Text Available This article focuses on the comparison of organizational antecedents of teachers' fostering of students' effort in two quite different accountability regimes: one management regime with an external-accountability system and one with no external accountability devices. The methodology involves cross-sectional surveys from two different management systems: (1 teachers working under assessment-based accountability (N = 236 and (2 folk–high school teachers who work without tests and examinations and, thereby, without external accountability devices (N = 366. The purpose of the study is to estimate the path coefficients in structural equation modeling in the two regimes and compare the significance of relationships between concepts in the structural models. Through this comparison, inferences are drawn suggesting how accountability repercussions and other leadership organizational antecedents may influence teachers' fostering of students' efforts and how qualitative aspects among school professionals may influence the fostering of effort. Implications for practice and directions for future research are discussed.Christopherson, K, Elstad, E, & Turmo, A. (2012. Antecedents of teachers fostering effort within two different management regimes: an assessment-based accountability regime and regime without external pressure on results. International Journal of Education Policy and Leadership 7(6. Retrieved from www.ijepl.org.

  15. Plasma Physics Regimes in Tokamaks with Li Walls

    Low recycling regimes with a plasma limited by a lithium wall surface suggest enhanced stability and energy confinement, both necessary for tokamak reactors. These regimes could make ignition feasible in compact tokamaks. Ignited Spherical Tokamaks (IST), self-sufficient in the bootstrap current, are introduced as a necessary step for development of the physics and technology of power reactors

  16. Disciplinary Regimes of "Care" and Complementary Alternative Education

    Thomson, Pat; Pennacchia, Jodie

    2016-01-01

    In schools, the notion of "care" is often synonymous with welfare and disciplinary regimes. Drawing on Foucault, and a study of alternative education (AE) across the UK, and looking in depth at two cases of complementary AE, we identify three types of disciplinary regimes at work in schools: (1) dominant performative reward and…

  17. Transition to ballistic regime for heat transport in helium II

    The size-dependent and flux-dependent effective thermal conductivity of narrow capillaries filled with superfluid helium is analyzed from a thermodynamic continuum perspective. The classical Landau evaluation of the effective thermal conductivity of quiescent superfluid, or the Gorter–Mellinck regime of turbulent superfluids, is extended to describe the transition to ballistic regime in narrow channels wherein the radius R is comparable to (or smaller than) the phonon mean-free path ℓ in superfluid helium. To do so, we start from an extended equation for the heat flux incorporating non-local terms, and take into consideration a heat slip flow along the walls of the tube. This leads from an effective thermal conductivity proportional to R2 (Landau regime) to another one proportional to Rℓ (ballistic regime). We consider two kinds of flows: along cylindrical pipes and along two infinite parallel plates. - Highlights: • Heat transport in counterflow helium in the ballistic regime. • The one-fluid model based on the Extended Thermodynamics is used. • The transition from the Landau regime to the ballistic regime. • The transition from quantum turbulence to ballistic regime

  18. Molecular dynamics of flows in the Knudsen regime

    Cieplak, Marek; Koplik, Joel; Banavar, Jayanth R.

    2000-01-01

    Novel technological applications often involve fluid flows in the Knudsen regime in which the mean free path is comparable to the system size. We use molecular dynamics simulations to study the transition between the dilute gas and the dense fluid regimes as the fluid density is increased.

  19. Extractive Regimes: Toward a Better Understanding of Indonesian Development

    Gellert, Paul K.

    2010-01-01

    This article proposes the concept of an extractive regime to understand Indonesia's developmental trajectory from 1966 to 1998. The concept contributes to world-systems, globalization, and commodity-based approaches to understanding peripheral development. An extractive regime is defined by its reliance on extraction of multiple natural resources…

  20. Transition to ballistic regime for heat transport in helium II

    Sciacca, Michele, E-mail: michele.sciacca@unipa.it [Dipartimento Scienze Agrarie e Forestali, Università degli studi di Palermo, Viale delle Scienze, 90128 Palermo (Italy); Departament de Física, Universitat Autònoma de Barcelona, 08193 Bellaterra, Catalonia (Spain); Sellitto, Antonio, E-mail: ant.sellitto@gmail.com [Dipartimento di Matematica, Informatica ed Economia, Università della Basilicata, Campus Macchia Romana, 85100 Potenza (Italy); Jou, David, E-mail: david.jou@uab.cat [Departament de Física, Universitat Autònoma de Barcelona, 08193 Bellaterra, Catalonia (Spain); Institut d' Estudis Catalans, Carme 47, 08001 Barcelona, Catalonia (Spain)

    2014-07-04

    The size-dependent and flux-dependent effective thermal conductivity of narrow capillaries filled with superfluid helium is analyzed from a thermodynamic continuum perspective. The classical Landau evaluation of the effective thermal conductivity of quiescent superfluid, or the Gorter–Mellinck regime of turbulent superfluids, is extended to describe the transition to ballistic regime in narrow channels wherein the radius R is comparable to (or smaller than) the phonon mean-free path ℓ in superfluid helium. To do so, we start from an extended equation for the heat flux incorporating non-local terms, and take into consideration a heat slip flow along the walls of the tube. This leads from an effective thermal conductivity proportional to R{sup 2} (Landau regime) to another one proportional to Rℓ (ballistic regime). We consider two kinds of flows: along cylindrical pipes and along two infinite parallel plates. - Highlights: • Heat transport in counterflow helium in the ballistic regime. • The one-fluid model based on the Extended Thermodynamics is used. • The transition from the Landau regime to the ballistic regime. • The transition from quantum turbulence to ballistic regime.

  1. Bargaining among Nations: Culture, History, and Perceptions in Regime Formation.

    Lipschutz, Ronnie D.

    1991-01-01

    The formation of regimes (collective international schemes) for managing global problems depends on culture, history, and perceptions. The ways in which these elements affect bargaining among nations over issues of the global commons are discussed. Implications are reviewed for a regime to deal with atmospheric conditions and global warming. (SLD)

  2. Policy Ideas, Knowledge Regimes and Comparative Political Economy

    Campbell, John L.; Pedersen, Ove K.

    2015-01-01

    Despite much attention to how ideas affect policy making, where these ideas come from is a blind spot in comparative political economy. We show that an important source of policy ideas are knowledge regimes—fields of policy research organizations. We show as well that the organization of knowledge...... regimes is heavily influenced by the organization of their surrounding political economies such that knowledge regimes have particular national characters. Furthermore, when people perceive that the utility of their knowledge regime for the rest of the political economy breaks down, they often try to...... restore it by transforming their knowledge regimes, albeit in ways that are still shaped by the surrounding political and economic institutions. The effectiveness of their efforts is not guaranteed. The argument is based on an analysis of the evolution of knowledge regimes since the 1970s in the USA...

  3. Mercury's thermal evolution and core crystallization regime

    Rivoldini, A.; Van Hoolst, T.; Dumberry, M.; Steinle-Neumann, G.

    2015-10-01

    Unlike the Earth, where the liquid core isentrope is shallower than the core liquidus, at the lower pressures inside Mercury's core the isentrope can be steeper than the melting temperature. As a consequence, upon cooling, the isentrope may first enter a solid stability field near the core mantle boundary and produce ironrich snow that sinks under gravity and produces buoyant upwellings of iron depleted fluid. Similar to bottom up crystallization, crystallization initiated near the top might generate sufficient buoyancy flux to drive magnetic field generation by compositional convection.In this study we model Mercury's thermal evolution by taking into account the formation of iron-rich snow to assess when the conditions for an internally magnetic field can be satisfied. We employ a thermodynamic consistent description of the iron high-pressure phase diagram and thermoelastic properties of iron alloys as well as the most recent data about the thermal conductivity of core materials. We use a 1-dimensional parametrized thermal evolution model in the stagnant lid regime for the mantle (e.g. [1]) that is coupled to the core. The model for the mantle takes into account the formation of the crust due to melting at depth. Mantle convection is driven by heat producing radioactive elements, heat loss from secular cooling and from the heat supplied by the core. The heat generated inside the core is mainly provided from secular cooling, from the latent heat released at iron freezing, and from gravitational energy resulting form the release of light elements at the inner core-outer core boundary as well as from the sinking of iron-rich snow and subsequent upwellings of light elements in the snow zone. If the heat flow out of the core is smaller than the heat transported along the core isentrope a thermal boundary will from at the top of the outer core. To determine the extension of the convecting region inside the liquid core we calculate the convective power [2]. Finally, we

  4. States agree on stronger physical protection regime

    Full text: Delegates from 89 countries agreed on 8 July to fundamental changes that will substantially strengthen the Convention on the Physical Protection of Nuclear Material (CPPNM). IAEA Director General Mohamed ElBaradei welcomed the agreement in saying 'This new and stronger treaty is an important step towards greater nuclear security by combating, preventing, and ultimately punishing those who would engage in nuclear theft, sabotage or even terrorism. It demonstrates that there is indeed a global commitment to remedy weaknesses in our nuclear security regime.' The amended CPPNM makes it legally binding for States Parties to protect nuclear facilities and material in peaceful domestic use, storage as well as transport. It will also provide for expanded cooperation between and among States regarding rapid measures to locate and recover stolen or smuggled nuclear material, mitigate any radiological consequences of sabotage, and prevent and combat related offences. The original CPPNM applied only to nuclear material in international transport. Conference President Dr. Alec Baer said 'All 89 delegations demonstrated real unity of purpose. They put aside some very genuine national concerns in favour of the global interest and the result is a much improved convention that is better suited to addressing the nuclear security challenges we currently face.' The new rules will come into effect once they have been ratified by two-thirds of the 112 States Parties of the Convention, expected to take several years. 'But concrete actions are already taking place around the world. For more than 3 years, the IAEA has been implementing a systematic Nuclear Security plan, including physical protection activities designed to prevent, detect and respond to malicious acts,' said Anita Nillson, Director of the IAEA's Office of Nuclear Security. The Agency's Nuclear Security Fund, set up after the events of 9/11, has delivered $19.5 million in practical assistance to 121 countries

  5. Human influence on California fire regimes.

    Syphard, Alexandra D; Radeloff, Volker C; Keeley, Jon E; Hawbaker, Todd J; Clayton, Murray K; Stewart, Susan I; Hammer, Roger B

    2007-07-01

    Periodic wildfire maintains the integrity and species composition of many ecosystems, including the mediterranean-climate shrublands of California. However, human activities alter natural fire regimes, which can lead to cascading ecological effects. Increased human ignitions at the wildland-urban interface (WUI) have recently gained attention, but fire activity and risk are typically estimated using only biophysical variables. Our goal was to determine how humans influence fire in California and to examine whether this influence was linear, by relating contemporary (2000) and historic (1960-2000) fire data to both human and biophysical variables. Data for the human variables included fine-resolution maps of the WUI produced using housing density and land cover data. Interface WUI, where development abuts wildland vegetation, was differentiated from intermix WUI, where development intermingles with wildland vegetation. Additional explanatory variables included distance to WUI, population density, road density, vegetation type, and ecoregion. All data were summarized at the county level and analyzed using bivariate and multiple regression methods. We found highly significant relationships between humans and fire on the contemporary landscape, and our models explained fire frequency (R2 = 0.72) better than area burned (R2 = 0.50). Population density, intermix WUI, and distance to WUI explained the most variability in fire frequency, suggesting that the spatial pattern of development may be an important variable to consider when estimating fire risk. We found nonlinear effects such that fire frequency and area burned were highest at intermediate levels of human activity, but declined beyond certain thresholds. Human activities also explained change in fire frequency and area burned (1960-2000), but our models had greater explanatory power during the years 1960-1980, when there was more dramatic change in fire frequency. Understanding wildfire as a function of the

  6. DEMOCRACIES HEIR OF AUTHORITARIAN REGIMES AS DEMOCRACIAS HERDEIRAS DE REGIMES AUTORITÁRIOS

    Edson Luis de Almeida Teles

    2008-04-01

    Full Text Available the objective of this article, making use of the theory of Hannah
    Arendt, is to show as the disruption with regimes of exception if it effected by means of transistions, of a vision of the politics as confrontation and violence for a model of the consensus and its counterpart was to occult the divergent ways with that the social subjetividades breach with the rational model, overshadowing the interpretations of the memory in the democratic normalizationo objetivo deste artigo, fazendo uso da teoria de Hannah Arendt,
    é mostrar como o rompimento com os regimes de exceção efetuou-se, por meio de transições, de uma visão da política como enfrentamento e violência, para um modelo do consenso, e sua contrapartida foi ocultar os modos divergentes com que as subjetividades sociais rompem com o modelo racional, obscurecendo as interpretações da memória na normalização democrática.

  7. Boundary between Stable and Unstable Regimes of Accretion. Ordered and Chaotic Unstable Regimes

    Blinova, A A; Lovelace, R V E

    2015-01-01

    We search for the boundary between stable and Rayleigh-Taylor unstable regimes of accretion to magnetized stars in a new set of high grid resolution simulations. We found that the boundary between stable and unstable regimes is mainly determined by the ratio of the corotation radius r_cor (where the Keplerian angular velocity in the disc matches the angular velocity of the star) to the magnetospheric radius r_m (where the magnetic stress in the magnetosphere matches the matter stress in the disc). Instability is stronger when r_cor is larger with respect to r_m, that is, when the gravitational force is larger than the centrifugal force at the inner disc. In the cases of a small tilt of the magnetosphere, Theta=5 deg, and a small alpha-parameter of viscosity, alpha=0.02, the boundary is located at r_cor approx. 1.4 r_m. Instability becomes stronger at higher values of viscosity, and occurs at lower values of r_cor/r_m. At higher values of Theta, the variability associated with instability decreases. Simulation...

  8. Modeling Non-robust Verification Regimes: Three Case Studies

    The term verification assumed a prominent role with the Chemical Weapons Convention (CWC) and the Comprehensive Nuclear Test Ban Treaty (CTBT) with the specification of verification regimes in the text of the treaty. In previous treaties, the term was either absent or, as in the case of the Non-Proliferation Treaty (NPT), mentioned in some broader context, such as, a State 'undertakes to accept safeguards, for the exclusive purpose of verification' Following the trend set by the CWC and the CTBT, extensive discussions have been taking place in the Committee on Disarmament for specifying a verification regime for the Biological Weapons Convention (BWC). These discussions have yet to produce any results, because it is becoming apparent that a robust verification system, if it could be specified, would be complex, intrusive and costly. With a heightened concern about proliferation of weapons of mass destruction, return to a regime without some monitoring, evaluation and assessment is very unlikely. Between these two extremes lies the concept of confidence building measures. The data collected using such a concept, whether by declarations, sensing, inspections, or some combination of these monitoring regimes, would lead to ambiguous conclusions about compliance. If one defines as robust a verification regime that provides a high level of confidence (in the vicinity of 100%) that all States Parties to a treaty are in compliance, then, the absence of any monitoring would reduce the confidence level to zero. The question then arises, what would be the level of confidence under a regime of confidence building measures. The question is equally relevant for the regime of safeguards under INFCIRC/540. One then would expect that the confidence of any State Party about compliance of others would range from very low to very high depending on the information collected by the monitoring system. There has been little, if any, effort to develop mechanisms for assessing

  9. An Examination of the Nature of Global MODIS Cloud Regimes

    Oreopoulos, Lazaros; Cho, Nayeong; Lee, Dongmin; Kato, Seiji; Huffman, George J.

    2014-01-01

    We introduce global cloud regimes (previously also referred to as "weather states") derived from cloud retrievals that use measurements by the Moderate Resolution Imaging Spectroradiometer (MODIS) instrument aboard the Aqua and Terra satellites. The regimes are obtained by applying clustering analysis on joint histograms of retrieved cloud top pressure and cloud optical thickness. By employing a compositing approach on data sets from satellites and other sources, we examine regime structural and thermodynamical characteristics. We establish that the MODIS cloud regimes tend to form in distinct dynamical and thermodynamical environments and have diverse profiles of cloud fraction and water content. When compositing radiative fluxes from the Clouds and the Earth's Radiant Energy System instrument and surface precipitation from the Global Precipitation Climatology Project, we find that regimes with a radiative warming effect on the atmosphere also produce the largest implied latent heat. Taken as a whole, the results of the study corroborate the usefulness of the cloud regime concept, reaffirm the fundamental nature of the regimes as appropriate building blocks for cloud system classification, clarify their association with standard cloud types, and underscore their distinct radiative and hydrological signatures.

  10. Multifractal regime detecting method for financial time series

    Highlights: • A multifractal regime detecting method (MRDM) introduced based on generalized Hurst exponent. • Multifractal regimes in the KOSPI from 1990 through 2012 are identified. • Surrogated tests are performed for the validation of MRDM. - Abstract: We focus on time varying multifractality in time series and introduce a multifractal regime detecting method (MRDM) adopting a nonparametric statistical test for multifractality based on generalized Hurst exponent (GHE). MRDM is a practical method to discriminate multifractal regimes in a time series of any length using a moving time window approach with the adjustable time window size and the moving interval. MRDM is applied to simulations consisting of both multifractal and monofractal regimes, and the results confirm its validity. Using MRDM, we identify multifractal regimes in the time series of Korea composite stock price index (KOSPI) from 1990 through 2012 and observe the distinct stylized facts of the KOSPI return values in multifractal regimes such as the heavy tail distribution, high kurtosis, and the long memory in volatility. Surrogate tests based on improved amplitude adjusted Fourier transformation (IAAFT) algorithm, normal distribution, and generalized student t distribution are performed for the validation of MDRM, and the probable causes of multifractality in the KOSPI series are discussed

  11. Marine regime shifts: drivers and impacts on ecosystems services

    Rocha, J.; Yletyinen, J.; Biggs, R.; Blenckner, T.; Peterson, G.

    2015-01-01

    Marine ecosystems can experience regime shifts, in which they shift from being organized around one set of mutually reinforcing structures and processes to another. Anthropogenic global change has broadly increased a wide variety of processes that can drive regime shifts. To assess the vulnerability of marine ecosystems to such shifts and their potential consequences, we reviewed the scientific literature for 13 types of marine regime shifts and used networks to conduct an analysis of co-occurrence of drivers and ecosystem service impacts. We found that regime shifts are caused by multiple drivers and have multiple consequences that co-occur in a non-random pattern. Drivers related to food production, climate change and coastal development are the most common co-occurring causes of regime shifts, while cultural services, biodiversity and primary production are the most common cluster of ecosystem services affected. These clusters prioritize sets of drivers for management and highlight the need for coordinated actions across multiple drivers and scales to reduce the risk of marine regime shifts. Managerial strategies are likely to fail if they only address well-understood or data-rich variables, and international cooperation and polycentric institutions will be critical to implement and coordinate action across the scales at which different drivers operate. By better understanding these underlying patterns, we hope to inform the development of managerial strategies to reduce the risk of high-impact marine regime shifts, especially for areas of the world where data are not available or monitoring programmes are not in place.

  12. Shearing box simulations in the Rayleigh unstable regime

    Nauman, Farrukh

    2015-01-01

    We study the stability properties of Rayleigh unstable flows both in the purely hydrodynamic and magnetohydrodynamic (MHD) regimes for two different values of the shear $q=2.1, 4.2$ ($q = - d\\ln\\Omega / d\\ln r$) and compare it with the Keplerian case $q=1.5$. The Rayleigh stability criterion states that hydrodynamic shear flows are stable for $q2$ regime as the volume averaged velocities ($k=0$ mode) are unstable in this regime but the advantage of using a pseudospectral code is that the $k=0$ mode is conserved. We find that the $q>2$ regime is unstable to turbulence both in the hydrodynamic and in the MHD limit (with an initially weak magnetic field). In the $q>2$ regime, the velocity fluctuations dominate the magnetic fluctuations whereas in the $q2$ regime the instability produces primarily velocity fluctuations that cause magnetic fluctuations, with the causality reversed for the $q<2$ MRI unstable regime. We also find that the magnetic field correlation is increasingly localized as the shear is increa...

  13. The NPT regime: Progress and promises

    Full text: Thesis. The 'NPT regime' has arrived at a fateful crossroads. Though extended indefinitely in 1995, its future is my no means secure. The future 'progress' of this treaty will depend upon whether the 'promises' of its States parties are fully implemented and, eventually, upon the treaty's success in achieving fully universal membership. Challenges The treaty faces many short-term and longer-term challenges: Short term - The first Preparatory Committee meeting for the 2005 Review Conference will meet next year. NNWS will want to see some evidence of progress on nuclear disarmament (Art. VI), along the lines prescribed in the 13 'practical steps' agreed at the last Review Conference. Yet progress has been set back by: uncertainties over the future of the ABM Treaty; the failure of START II and the CTBT to enter into force; the lack of a FISMAT treaty and a treaty establishing a NWFZ in Central Asia; continued qualitative improvements in nuclear weapons; hints that nuclear testing may one day resume; the persistence of doctrines of first-use, pre-emptive use, and use against states that use CBW. Other compliance-related questions will arise over safeguards (e.g. the inability of the IAEA to conduct inspections in the DPRK; signs of a breakdown of the norm of full-scope IAEA safeguards, e.g. in South Asia). There are also concerns over the implementation of non-proliferation commitments (e.g. persisting allegations about nuclear weapon programmes in existing NNWS). The terrorist attacks in New York and Washington, D.C. on 11 September should also serve as a reminder of the new terrorist dangers relating to the possible use of weapons of mass destruction and unorthodox delivery systems. Longer term - Selectivity in the enforcement of NPT norms; unilateralism; IAEA funding uncertainties and shortfalls; difficulties in reaching universal membership (India, Pakistan, and Israel); continuing compliance problems with respect to both non-proliferation and

  14. Considerations on nonproliferation regime meeting in a changing world

    Kurihara, Hiroyoshi; Kikuchi, Masahiro [Nuclear Material Control Center, Tokyo (Japan)

    1994-12-31

    This paper summarizes the past history of worldwide nonproliferation regime, then proposes the future improvements on the regime. Present worldwide nonproliferation regime have been formulated during the cold war era. Therefore, the structure and measures of the regime were heavily influenced by the features of cold war era. Though the cold war was over, still new international order does not seem to be on the horizon, we need to review the present regime and to improve the regime compatible to new world situation. Generally speaking, the nonproliferation regime have gained moderate success so far. We could point out the following features as a kind of success: (1) No increase of overt Nuclear Weapon State (NWS), (2) All five NWSs have finally participated to the NPT, (3) South Africa has destroyed its nuclear weapons and became Non-Nuclear Weapon State (NNWS), (4) Successful conclusions of some regional arrangements, such as Tlatelolco, Ralotonga, and (5) Strengthening of export control on sensitive items. On the other hand, we recognize the following points as the failures of the regime. (6) India, Pakistan and Israel reject to join the NPT, (7) Existence of some violation against NPT regime, i.e. Iraqi case and DPRK case, (8) Insufficient effective measures against brain drain problem, (9) Risk exists for the long term extension of NPT, and (10) Insufficient flexibility to meet changing boundary conditions. We would propose the various measures for strengthening to meet changing boundary conditions, as follows: (11) Measures to be taken along with future civil use of Plutonium, (12) Strengthening and rationalizing international safeguards, (13) Countermeasures for emerging new types of nuclear proliferation, (14) Strengthening nuclear material control in NWS, (15) Measures to be taken for nuclear material from dismantled nuclear weapons, and (16) Nuclear disarmament. (author).

  15. Considerations on nonproliferation regime meeting in a changing world

    This paper summarizes the past history of worldwide nonproliferation regime, then proposes the future improvements on the regime. Present worldwide nonproliferation regime have been formulated during the cold war era. Therefore, the structure and measures of the regime were heavily influenced by the features of cold war era. Though the cold war was over, still new international order does not seem to be on the horizon, we need to review the present regime and to improve the regime compatible to new world situation. Generally speaking, the nonproliferation regime have gained moderate success so far. We could point out the following features as a kind of success: 1) No increase of overt Nuclear Weapon State (NWS), 2) All five NWSs have finally participated to the NPT, 3) South Africa has destroyed its nuclear weapons and became Non-Nuclear Weapon State (NNWS), 4) Successful conclusions of some regional arrangements, such as Tlatelolco, Ralotonga, and 5) Strengthening of export control on sensitive items. On the other hand, we recognize the following points as the failures of the regime. 6) India, Pakistan and Israel reject to join the NPT, 7) Existence of some violation against NPT regime, i.e. Iraqi case and DPRK case, 8) Insufficient effective measures against brain drain problem, 9) Risk exists for the long term extension of NPT, and 10) Insufficient flexibility to meet changing boundary conditions. We would propose the various measures for strengthening to meet changing boundary conditions, as follows: 11) Measures to be taken along with future civil use of Plutonium, 12) Strengthening and rationalizing international safeguards, 13) Countermeasures for emerging new types of nuclear proliferation, 14) Strengthening nuclear material control in NWS, 15) Measures to be taken for nuclear material from dismantled nuclear weapons, and 16) Nuclear disarmament. (author)

  16. Raman amplification in the broken-wave regime

    Farmer, John P

    2015-01-01

    In regimes far beyond the wavebreaking theshold of Raman amplification, we show that significant amplifcation can occur after the onset of wavebreaking, before phase mixing destroys the coupling between pump and probe. The amplification efficiency in this regime is therefore strongly dependent on the energy-transfer rate when wavebreaking occurs, and is, as such, sensitive to both the probe amplitude and profile. In order to access the higher-efficiency broken-wave regime, a short, intense probe is required. Parameter scans show the marked difference in behaviour compared to below wavebreaking, where longer, more energetic pulses lead to improved efficiencies.

  17. Diversity-Multiplexing Tradeoff in the Low-SNR Regime

    Loyka, Sergey

    2011-01-01

    An extension of the popular diversity-multiplexing tradeoff framework to the low-SNR (or wideband) regime is proposed. The concept of diversity gain is shown to be redundant in this regime since the outage probability is SNR-independent and depends on the multiplexing gain and the channel power gain statistics only. The outage probability under the DMT framework is obtained in an explicit, closed form for a broad class of channels. The low and high-SNR regime boundaries are explicitly determined for the scalar Rayleigh-fading channel, indicating a significant limitation of the SNR-asymptotic DMT when the multiplexing gain is small.

  18. Formations and transformations of language regimes: Turkey, a case study

    Balçık, Mehmet Berk; Balcik, Mehmet Berk

    2009-01-01

    There are two main aims of this dissertation: to present a legal and ideological history of the formation of the language regime in Turkey in the Republican period; and to analyze its transformation in the post-1980 era. A language regime is defined in this dissertation as a de jure or de facto regulation of the linguistic behavior, in its content or in its status, within a space of communicative action, such as that of a nation-state or a speech group. In other words, a language regime is a ...

  19. Laserlike and atomlike regimes in a one-atom laser

    Boozer, A. D.

    2008-01-01

    We consider a three-level model of a one-atom laser, and show that there are two limiting regimes of parameter space, one in which the system behaves like a conventional laser and one in which the system exhibits novel quantum properties. We show that in the first limiting regime, the model can be approximated by semiclassical laser theory, and in the second limiting regime the model can be approximated as an effective two-level atom. We also perform numerical simulations that confirm the lim...

  20. Tidal regimes and salt marshes - the River Hamble analogue

    Construction of estuarine tidal-energy barrages has a potentially major effect on the tidal regime of the estuary, particularly upstream of a barrage. Because tidal regime largely controls the distribution and species composition of intertidal plant and animal communities, it is important to understand how barrages may affect such communities. The main objectives of the research described in this report were to relate recent changes in tidal regime within an embanked area of salt marsh and mudflat to changes in the distribution of plant species. This was to test predictions about tidal control of species' range and to assess the site's suitability as an analogue of post-barrage conditions. (author)

  1. Nonlinear Regime of a Single-mode CSR Instability

    Close to the shielding threshold of synchrotron radiation in a toroidal waveguide the coherent synchrotron radiation (CSR) instability may be driven by a single synchronous mode. The linear analysis of the problem is presented elsewhere. In this paper we study a nonlinear regime of the single-mode CSR instability. We show that in the nonlinear regime the instability is described by a system of equations which, after a proper scaling, coincides with the 1D FEL instability theory. We present numeric and analytic analysis of the non-linear regime for the time interval large compared with the damping time taking into account radiation damping and diffusion due to the quantum fluctuations

  2. Information management in NACD regimes: a comparative analysis

    While all non-proliferation, arms control and disarmament (NACD) regimes must address the issue of information management, this area has remained an under-explored part of the arms control field. This paper compares information management processes across a variety of NACD regimes for the purpose of identifying potential synergies between regimes and suggesting means by which to strengthen future arms control verification efforts. The paper explores the information management systems of the International Atomic Energy Agency (IAEA), the United Nations Special Commission in Iraq (UNSCOM), the Conventional Forces in Europe Agreement (CFE), and the Comprehensive Test Ban Treaty (CTBT). (author)

  3. Raman amplification in the coherent wave-breaking regime.

    Farmer, J P; Pukhov, A

    2015-12-01

    In regimes far beyond the wave-breaking threshold of Raman amplification, we show that significant amplification can occur after the onset of wave breaking, before phase mixing destroys the coherent coupling between pump, probe, and plasma wave. Amplification in this regime is therefore a transient effect, with the higher-efficiency "coherent wave-breaking" (CWB) regime accessed by using a short, intense probe. Parameter scans illustrate the marked difference in behavior between below wave breaking, in which the energy-transfer efficiency is high but total energy transfer is low, wave breaking, in which efficiency is low, and CWB, in which moderate efficiencies allow the highest total energy transfer. PMID:26764840

  4. Regime-Switching Risk: To Price or Not to Price?

    Tak Kuen Siu

    2011-01-01

    “normative” issues to be addressed in pricing contingent claims under a Markovian, regime-switching, Black-Scholes-Merton model. We address this issue using a minimal relative entropy approach. Firstly, we apply a martingale representation for a double martingale to characterize the canonical space of equivalent martingale measures which may be viewed as the largest space of equivalent martingale measures to incorporate both the diffusion risk and the regime-switching risk. Then we show that an optimal equivalent martingale measure over the canonical space selected by minimizing the relative entropy between an equivalent martingale measure and the real-world probability measure does not price the regime-switching risk. The optimal measure also justifies the use of the Esscher transform for option valuation in the regime-switching market.

  5. Optimization of Normal Regimes of Power Transmission Lines

    E. G. Pospelov

    2014-06-01

    Full Text Available In order to optimize normal regimes of the power transmission lines notions of economic, maximum and the largest efficiency of a transmission line are used. Power losses by wire corona transmission losses are considered.

  6. Optimization of Normal Regimes of Power Transmission Lines

    E. G. Pospelov; Pospelov, G. E.(Max-Planck-Institut für Physik (Werner-Heisenberg-Institut), München, Germany)

    2014-01-01

    In order to optimize normal regimes of the power transmission lines notions of economic, maximum and the largest efficiency of a transmission line are used. Power losses by wire corona transmission losses are considered.

  7. Stochastic regimes in very-low-frequency fluidic oscillator

    Tesař, Václav

    2016-03-01

    Paper discusses interesting unexpected stochastic regimes discovered in a fluidic oscillator designed for operation at very low oscillation frequencies - without the inconvenience of the long feedback loops needed in standard low-frequency oscillator designs. The new oscillator contains a pair of bistable turn-down active valves operating in anti-parallel — essentially analogous to Abraham & Bloch electric "multibrateur" invented in 1919. Three different self-excited oscillation regimes were found. In the order of increasing supplied flow rate, these regimes are characterised by: (A) generation of stochastic-duration multi-pulse packs, (B) generation of individual pulses with a degree of periodicity, and (C) regime with randomly appearing flow pulses separated by intervals of the order of seconds.

  8. Determination of three characteristic regimes of weakly charged polyelectrolytes monolayers

    We have demonstrated that monolayer films of randomly charged polystyrene sulfonated acid (PSSA) can be produced by the Langmuir technique, and observed the micro-domain structures, produced by the phase separation of electrostatically charged moieties and the hydrophobic moieties. Using atomic force microscopy and Langmuir isotherm, we found three specific regimes for the polyelectrolytes with various degrees of sulfonation (4-35%); very low charged PSSA (4-5%) in the hydrophobic regime, moderately charged PSSA (6-16%) which possessed a well-balanced nature between electrostatic and the hydrophobic interactions, and strongly amphiphilic nature of PSSA (6-16%) in the ionomer regime. Finally, we could categorize PSSA 35% in the polyelectrolyte regime, due to the dominance of the electrostatic interactions over the hydrophobic interactions

  9. Nonequilibrium electron transport through quantum dots in the Kondo regime

    Wölfle, Peter; Paaske, Jens; Rosch, Achim; Kroha, Johann

    2005-01-01

    Electron transport at large bias voltage through quantum dots in the Kondo regime is described within the perturbative renormalization group extended to nonequilibrium. The conductance, local magnetization, dynamical spin susceptibility and local spectral function are calculated. We show how the ...

  10. The Hadley and Rossby regimes in a spherical atmosphere

    Feldstein, S. B.; Clark, J. H. E.

    1985-01-01

    The properties of the steady Hadley and Rossby regimes for a thermally forced rotating fluid on a sphere are studied. The two layer modified geostrophic model is employed which allows for thermal advection by the divergent wind and time dependent static stability. Heating processes are parameterized using the Newtonian approximation and Rayleigh friction is accounted for. The equations are transformed to spectral form using spherical harmonics and then truncated retaining a simple axisymmetric state and initial, one wave. A time independent Hadley circulation is obtained which is neutral to axisymmetric disturbances but unstable to wave like perturbations for intermediate values of the meridional temperature gradient, indicating the existence of both an upper and lower symmetric Hadley regime. An analytical solution for the steady Rossby circulation is determined for values of the meridional temperature gradient where the Hadley regime is unstable. Linear perturbation theory is used to show that within the steady Rossby regime two or more waves cannot exist simultaneously.

  11. Evolving International Trade and Monetary Regimes and Related Policy Issues

    Kumiharu Shigehara

    1991-01-01

    This essay focuses on some aspects of policy issues relating to evolving international trade and monetary regimes, and patterns of national saving and investment balances conducive to better global economic performance.

  12. Spacetime-noncommutativity regime of Loop Quantum Gravity

    Amelino-Camelia, Giovanni; Ronco, Michele; Cesarini, Lorenzo; Lecian, Orchidea Maria

    2016-01-01

    A recent study by Bojowald and Paily provided a path toward the identification of an effective quantum-spacetime picture of Loop Quantum Gravity, applicable in the "Minkowski regime", the regime where the large-scale (coarse-grained) spacetime metric is flat. A pivotal role in the analysis is played by Loop-Quantum-Gravity-based modifications to the hypersurface deformation algebra, which leave a trace in the Minkowski regime. We here show that the symmetry-algebra results reported by Bojowald and Paily are consistent with a description of spacetime in the Minkowski regime given in terms of the $\\kappa$-Minkowski noncommutative spacetime, whose relevance for the study of the quantum-gravity problem had already been proposed for independent reasons.

  13. Capillary Breakup of a Liquid Bridge: Identifying Regimes and Transitions

    Li, Yuan

    2016-01-01

    Computations of the breakup of a liquid bridge are used to establish the limits of applicability of similarity solutions derived for different breakup regimes. These regimes are based on particular viscous-inertial balances, that is different limits of the Ohnesorge number $Oh$. To accurately establish the transitions between regimes, the minimum bridge radius is resolved through four orders of magnitude using a purpose-built multiscale finite element method. This allows us to construct a quantitative phase diagram for the breakup phenomenon which includes the appearance of a recently discovered low-$Oh$ viscous regime. The method used to quantify the accuracy of the similarity solutions allows us to identify a number of previously unobserved features of the breakup, most notably an oscillatory convergence towards the viscous-inertial similarity solution. Finally, we discuss how the new findings open up a number of challenges for both theoretical and experimental analysis.

  14. Tumor marker utility and prognostic relevance of cathepsin B, cathepsin L, urokinase-type plasminogen activator, plasminogen activator inhibitor type-1, CEA and CA 19-9 in colorectal cancer

    Cathepsin B and L (CATB, CATL), urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 play an important role in colorectal cancer invasion. The tumor marker utility and prognostic relevance of these proteases have not been evaluated in the same experimental setting and compared with that of CEA and CA-19-9. Protease, CEA and CA 19-9 serum or plasma levels were determined in 56 patients with colorectal cancer, 25 patients with ulcerative colitis, 26 patients with colorectal adenomas and 35 tumor-free control patients. Protease, CEA, CA 19-9 levels have been determined by ELISA and electrochemiluminescence immunoassay, respectively; their sensitivity, specificity, diagnostic accuracy have been calculated and correlated with clinicopathological staging. The protease antigen levels were significantly higher in colorectal cancer compared with other groups. Sensitivity of PAI-1 (94%), CATB (82%), uPA (69%), CATL (41%) were higher than those of CEA or CA 19-9 (30% and 18%, respectively). PAI-1, CATB and uPA demonstrated a better accuracy than CEA or CA 19-9. A combination of PAI-1 with CATB or uPA exhibited the highest sensitivity value (98%). High CATB, PAI-1, CEA and CA 19-9 levels correlated with advanced Dukes stages. CATB (P = 0.0004), CATL (P = 0.02), PAI-1 (P = 0.01) and CA 19-9 (P = 0.004) had a significant prognostic impact. PAI-1 (P = 0.001), CATB (P = 0.04) and CA 19-9 (P = 0.02) proved as independent prognostic variables. At the time of clinical detection proteases are more sensitive indicators for colorectal cancer than the commonly used tumor markers. Determinations of CATB, CATL and PAI-1 have a major prognostic impact in patients with colorectal cancer

  15. Quantifying flooding regime in floodplain forests to guide river restoration

    Christian O. Marks; Keith H Nislow; Magilligan, Francis J.

    2014-01-01

    Abstract Determining the flooding regime needed to support distinctive floodplain forests is essential for effective river conservation under the ubiquitous human alteration of river flows characteristic of the Anthropocene Era. At over 100 sites throughout the Connecticut River basin, the largest river system in New England, we characterized species composition, valley and channel morphology, and hydrologic regime to define conditions promoting distinct floodplain forest assemblages. Species...

  16. Laboratory experiment on boundaries of upper stage plane bed regime

    Zrostlík, Štěpán; Matoušek, Václav

    2016-04-01

    Results are discussed of laboratory experiments on criteria determining the transition between the regime of dunes and the upper stage plane bed (UPB) regime and the transition between the UPB regime and the regime of wavy flow. The experiments were carried for 3 fractions of plastic material and two fractions of glass beads in a broad range of flow conditions (different discharges of water and solids and longitudinal bed slopes) in a tilting flume. The experiments reveal that, contrary to expectations, a constant value of the Shields parameter is not an appropriate criterion for the transition between the dune regime and the UPB regime. Furthermore, the transition appears to be insensitive to the total discharge of solids and water. Instead, the criterion seems to be well represented by a constant value of the average transport concentration of sediment (the ratio of volumetric discharge of solids and volumetric discharge of mixture). The experimental results exhibit a very tight correlation between the transport concentration and the longitudinal bed slope. Hence, a constant value of the bed slope can be considered an appropriate criterion for the transition. The transition between the UPB regime and the wavy regime (significant waves develop but they are not always standing waves) is found at a constant value of Froude number, which is in agreement with literature, although it is found at a higher value than the literature usually suggests (Fr = 1.2 instead of 1.0). Hence, the transition occurs in the super-critical flow but it is not necessarily associated with the critical flow.

  17. Brownian colloids in underdamped and overdamped regimes with nonhomogeneous temperature

    Sancho, J. M.

    2015-12-01

    The motion of Brownian particles when temperature is spatially dependent is studied by stochastic simulations and theoretical analysis. Nonequilibrium steady probability distributions Ps t(z ,v ) for both underdamped and overdamped regimes are analyzed. The existence of local kinetic energy equipartition theorem is also discussed. The transition between both regimes is characterized by a dimensionless friction parameter. This study is applied to three physical systems of colloidal particles.

  18. Patent regimes, firms and the commodification of knowledge

    Benjamin Coriat; Olivier Weinstein

    2011-01-01

    This paper analyses the evolution of the intellectual property regime (IPR), and more precisely the patent regime, in the USA since the 19-th century. To do so, we consider intellectual property laws within the context of wider changes in capitalism, focusing on two main historical phases: firstly, the period covering the formation and development of 'corporate capitalism' dominated by large corporations and then the new phase, which opened up in the 1980s, marked by the rise to power of fina...

  19. Capital Controls and the Timing of Exchange Regime Collapse

    Daekuen Park; Jeffrey Sachs

    1987-01-01

    This paper investigates the nature of balance of payments crises in regimes with capital controls. It extends earlier work on capital controls by assuming that households manage their consumption and asset portfolios to maximize intertemporal utility. Our main result is that capital controls are effective in delaying, but not preventing, a breakdown of a fixed exchange rate regime in the presence of money-financed fiscal deficits.

  20. Constructing a Transnational Timber Legality Assurance Regime: Architecture, Accomplishments, Challenges

    Overdevest, C.; Zeitlin, J

    2013-01-01

    The emerging transnational timber legality assurance regime comprises a set of interrelated policy instruments, both public and private, aimed at controlling trade in illegally logged wood and wood products. The potentially productive interactions among these instruments in the emerging forestry regime create prospects for engendering learning, stimulating cross-fertilization, and enhancing accountability. In this article, we analyze the EU's Forest Law Enforcement Governance and Trade (FLEGT...

  1. The Limited Viability of Dual Exchange-Rate Regimes

    Jacob A. Frenkel; Assaf Razin

    1986-01-01

    This paper examines the viability of dual exchange-rate regimes. Typically, under such a regime the exchange rates applicable to current-account(commercial) transactions and to capital-account (financial) transactions differ from each other. This difference may be determined in the free market if the authorities peg the commercial exchange rate and set a binding quota on external borrowing, or it may result from direct pegging of both exchange rates. The analysis starts with a specification o...

  2. A Private Property Rights Regime to Replenish a Groundwater Aquifer

    Bill Provencher

    1993-01-01

    Groundwater management is often reactive, and in some cases the groundwater stock (groundwater table) of an aquifer may fall below its optimal steady-state level before any thought is given to management. This paper examines a private property rights regime to restore a groundwater resource to its optimal steady-state. Results from a stochastic dynamic programming model of Madera County, California show that the private property rights regime recovers about 95 percent of the potential gain fr...

  3. Real Output and Prices Adjustments under Different Exchange Rate Regimes

    Rajmund MIRDALA

    2013-01-01

    Exchange rate regimes evolution in the European transition economies refers to one of the most crucial policy decision in the beginning of the 1990s employed during the initial stages of the transition process. During the period of last two decades we may identify some crucial milestones in the exchange rate regimes evolution in the European transition economies. due to existing diversity in exchange rate arrangements in the European transition economies in the pre-ERM2 period there seems to ...

  4. Shock-Induced Flows through Packed Beds: Transient Regimes

    Shtemler, Yuri M; Britan, Alex

    2006-01-01

    The early stage of the transient regimes in the shock-induced flows within solid-packed beds are investigated in the linear longwave and high-frequency approximation. The transient resistance law is refined as the Duhameltime integral that follows from the general concept of dynamic tortuosity and compressibility of the packed beds. A closed-form solution is expected to describe accurately the early stage of the transient regime flow and is in qualitative agreement with available experimental data.

  5. Generating Schemes for Long Memory Processes: Regimes, Aggregation and Linearity

    Davidson, James; Sibbertsen, Philipp

    2002-01-01

    This paper analyses a class of nonlinear time series models exhibiting long memory. These processes exhibit short memory fluctuations around a local mean (regime) which switches randomly such that the durations of the regimes follow a power law. We show that if a large number of independent copies of such a process are aggregated, the resulting processes are Gaussian, have a linear representation, and converge after normalisation to fractional Brownian motion. Two cases arise, a stationary ca...

  6. Were there regime switches in U.S. monetary policy?

    Christopher A. Sims; Zha, Tao

    2004-01-01

    A multivariate model, identifying monetary policy and allowing for simultaneity and regime switching in coefficients and variances, is confronted with U.S. data since 1959. The best fit is with a model that allows time variation in structural disturbance variances only. Among models that also allow for changes in equation coefficients, the best fit is for a model that allows coefficients to change only in the monetary policy rule. That model allows switching among three main regimes and one r...

  7. The market manipulation regime in the European Union

    Li, Xue

    2015-01-01

    Market manipulation is an illegal practice that enables a person can profit from practices that artificially raise or lower the prices of an instrument in the financial markets. Its prohibition is based on the 2003 Market Abuse Directive in the EU. The current market manipulation regime was broadly considered as a big success except for enforcement and supervisory inconsistencies in the Member States at the initial. A review of the market manipulation regime began at the end of 2007, which...

  8. PARTICULARITIES OF PARLIAMENTARY OVERSIGHT IN DIFFERENT POLITICAL REGIMES

    Silvia-Claudia CĂLIN-MIHALCEA

    2015-01-01

    The quality and intensity of the parliamentary oversight performed over the Government are shaped by several major criteria: political regime, electoral system, structure of the Parliament (unicameral/bicameral), parliamentary culture and tradition. This paper emphasizes some distinctive elements and particular mechanisms of the control exercised over the activities of the executive power, from the point of view of the political regime established in states with modern democracies.

  9. A critical review of the Chilean civil nuclear liability regime

    This article reviews the Chilean civil nuclear liability regime. The Nuclear Security Act (Law 18.302), enacted in 1984, and the Vienna Convention on Civil Liability for Nuclear Damage, ratified ed by Chile in 1989, are the fundamental laws of the current regime. Although Chile has no nuclear power plants, it is still important to analyze how the Chilean legislation would protect citizens from nuclear damages. This paper does not consider the policy reasons for and against the promotion of atomic energy. Rather, it critically examines the current status of the Chilean nuclear regime. Undoubtedly, if in the future Chile chooses to include nuclear sources in its energy mix, it will not be enough to introduce some isolated legal amendments, but it will be necessary to build a new Chilean Energy Regime which includes nuclear energy. In that scenario, though, it will be useful to know and understand how the current nuclear liability regime works. From this point of view, the reforms this article proposes to the current nuclear liability regime might be helpful to academics and policy makers alike

  10. Development of Risk Assessment Methodology for State's Nuclear Security Regime

    Threats of nuclear terrorism are increasing after 9/11 terrorist attack. Treats include nuclear explosive device (NED) made by terrorist groups, radiological damage caused by a sabotage aiming nuclear facilities, and radiological dispersion device (RDD), which is also called 'dirty bomb'. In 9/11, Al Qaeda planed to cause radiological consequences by the crash of a nuclear power plant and the captured airplane. The evidence of a dirty bomb experiment was found in Afganistan by the UK intelligence agency. Thus, the international communities including the IAEA work substantial efforts. The leaders of 47 nations attended the 2010 nuclear security summit hosted by President Obama, while the next global nuclear summit will be held in Seoul, 2012. Most states established and are maintaining state's nuclear security regime because of the increasing threat and the international obligations. However, each state's nuclear security regime is different and depends on the state's environment. The methodology for the assessment of state's nuclear security regime is necessary to design and implement an efficient nuclear security regime, and to figure out weak points. The IAEA's INPRO project suggests a checklist method for State's nuclear security regime. The IAEA is now researching more quantitative methods cooperatively with several countries including Korea. In this abstract, methodologies to evaluate state's nuclear security regime by risk assessment are addressed

  11. International regime formation: Ozone depletion and global climate change

    Two theoretical perspectives, neorealism and neoliberal institutionalism, dominate in international relations. An assessment is made of whether these perspectives provide compelling explanations of why a regime with specific targets and timetables was formed for ozone depletion, while a regime with such specificity was not formed for global climate change. In so doing, the assumptions underlying neorealism and neoliberal institutionalism are examined. A preliminary assessment is offered of the policymaking and institutional bargaining process. Patterns of interstate behavior are evolving toward broader forms of cooperation, at least with regard to global environmental issues, although this process is both slow and cautious. State coalitions on specific issues are not yet powerful enough to create a strong community of states in which states are willing to devolve power to international institutions. It is shown that regime analysis is a useful analytic framework, but it should not be mistaken for theory. Regime analysis provides an organizational framework offering a set of questions regarding the principles and norms that govern cooperation and conflict in an issue area, and whether forces independent of states exist which affect the scope of state behavior. An examination of both neorealism and neoliberal institutionalism, embodied by four approaches to regime formation, demonstrates that neither has sufficient scope to account for contextual dynamics in either the ozone depletion or global climate change regime formation processes. 261 refs

  12. Direct numerical simulations of the dense regime of roughness

    MacDonald, Michael; Chan, Leon; Chung, Daniel; Hutchins, Nicholas; Ooi, Andrew

    2015-11-01

    We investigate the sparse and dense regimes of roughness using Direct Numerical Simulations (DNS) of turbulent flow over three-dimensional sinusoidal surfaces in the transitionally rough regime. The sparse regime is known to lead to an increase in the Hama roughness function, ΔU+ , as the roughness density increases, while the dense regime is associated with a decrease in ΔU+ as density increases. In this parametric study, the wavelength of the sinusoidal roughness elements is varied while the roughness height is fixed. The minimal-span channel is used, as the high cost of the grid would otherwise make the dense roughness simulations unattainable. It was found that the dense regime began at solidity values (frontal area divided by wall-parallel projected area) greater than 0.15, in agreement with the literature. An analysis of the mean momentum balance above the roughness reveals that the decrease in ΔU+ in the dense regime is due to a reduction in the Reynolds shear stress. This reduction is located just above the roughness crest in the near-wall region, and the difference in the energy spectra of streamwise velocity between smooth and dense roughness clearly demonstrates that this is at long streamwise length scales.

  13. Do family policy regimes matter for children's well-being?

    Engster, Daniel; Stensöta, Helena Olofsdotter

    2011-01-01

    Researchers have studied the impact of different welfare state regimes, and particularly family policy regimes, on gender equality. Very little research has been conducted, however, on the association between different family policy regimes and children's well-being. This article explores how the different family policy regimes of twenty OECD countries relate to children's well-being in the areas of child poverty, child mortality, and educational attainment and achievement. We focus specifically on three family policies: family cash and tax benefits, paid parenting leaves, and public child care support. Using panel data for the years 1995, 2000, and 2005, we test the association between these policies and child well-being while holding constant for a number of structural and policy variables. Our analysis shows that the dual-earner regimes, combining high levels of support for paid parenting leaves and public child care, are strongly associated with low levels of child poverty and child mortality. We find little long-term effect of family policies on educational achievement, but a significant positive correlation between high family policy support and higher educational attainment. We conclude that family policies have a significant impact on improving children's well-being, and that dual-earner regimes represent the best practice for promoting children's health and development. PMID:21692245

  14. Trends in the hydrologic regime of Alpine rivers

    Bard, Antoine; Renard, Benjamin; Lang, Michel; Giuntoli, Ignazio; Korck, Jane; Koboltschnig, Gernot; Janža, Mitja; d'Amico, Michele; Volken, David

    2015-10-01

    This paper describes a trend analysis performed on 177 streamflow time series collected over the Alps in Central Europe. The analysis covers several facets of the Alpine hydrologic regimes, including winter droughts and spring snowmelt flows, both in terms of severity and timing of occurrence. Statistical trend tests are applied at a local scale (i.e. on a site-by-site basis) and at a regional scale (seeking a common trend for sites within the same hydro-climatic region). The overall results indicate a trend toward less severe winter droughts, and consistent changes in the timing of snowmelt flows. However, a more in-depth analysis at the scale of hydro-climatic regimes reveals more contrasted changes. While most glacial- and snowmelt-dominated regimes show a decreasing trend in the severity of winter droughts, contrasted trends are found for mixed snowmelt-rainfall regimes in the Southeastern Alps. Changes in the timing of snowmelt flows (earlier start and increased duration of the snowmelt season) mostly affect glacial- and snowmelt-dominated regimes. Lastly, glacial regimes show an increase in the volume and the peak of snowmelt flows.

  15. Sensitivity of streamflows to hydroclimatic fluctuations: resilience and regime shifts

    Botter, Gianluca; Basso, Stefano; Rodriguez-Iturbe, Ignacio; Rinaldo, Andrea

    2016-04-01

    Landscape and climate alterations foreshadow global-scale shifts of river flow regimes. However, a theory that identifies the range of foreseen impacts on streamflows resulting from inhomogeneous forcings and sensitivity gradients across diverse regimes is lacking. In this contribution, we use a dimensionless index embedding simple climate and landscape attributes (the ratio of the mean interarrival of streamflow-producing rainfall events and the mean catchment response time) to discriminate erratic regimes with enhanced intra-seasonal streamflow variability from persistent regimes endowed with regular flow patterns. The proposed classification is successfully applied to 110 seasonal streamflow distributions observed in 44 catchments of the Alps and the United States, allowing the identification of emerging patterns in space and time. In the same framework, the impact of multi-scale fluctuations of the underlying climatic drivers (temperature, precipitation) on the streamflow distributions can be analyzed. Theoretical and empirical data show that erratic regimes, typical of rivers with low mean discharges, are highly resilient in that they hold a reduced sensitivity to variations in the external forcing. Specific temporal trajectories of streamflow distributions and flow regime shifts driven by land-cover change and rainfall patterns can be also evidenced. The approach developed offers an objective basis for the analysis and prediction of the impact of climate/landscape change on water resources.

  16. Consumption, investment and life insurance under different tax regimes

    Bruun, Kenneth

    2013-01-01

    We study the effects of introducing taxation in classical continuous-time optimization problems with utility from consumption, bequest and retirement savings. Inspired by actual tax favoured retirement savings programs, we formulate and solve the optimization problem for various tax regimes, and ...... analysis of the results based on realistic parameter values and regimes. Based on American and Danish tax regimes we estimate the values of existing retirement saving favouring to be 1 – 2 percent of lifetime income.......We study the effects of introducing taxation in classical continuous-time optimization problems with utility from consumption, bequest and retirement savings. Inspired by actual tax favoured retirement savings programs, we formulate and solve the optimization problem for various tax regimes, and...... compare tax effects on consumption/savings contributions, investment and purchase of life insurance under the regimes. The optimization problems have analytical solutions, which allow for easy comparison of tax effects under the different regimes. To substantiate the results we also present a numerical...

  17. Numerical simulation of transitions between back discharge regimes

    Jánský, Jaroslav; Bessières, Delphine; Paillol, Jean; Lemont, Florent

    2014-11-01

    This paper presents numerical simulations of transitions between back discharge regimes. Back discharge refers to any discharge initiated at or near a dielectric layer covering a passive electrode. In this work, a pinhole in a dielectric layer on a plane anode serves as a model for back discharge activity. We have studied transitions between back discharge regimes by varying the surface charge density on the dielectric layer and the electric field in front of the pinhole. From the variation of these two independent parameters, the back discharge regimes have been depicted as a mode diagram inspired by the experimental study of Masuda and Mizuno. The resulting diagram includes the different discharge regimes that are commonly observed in experiments. The propagation of a positive ionizing wave inside the pinhole toward its edge, and the resulting formation of a plasma zone at its exit constitute the onset stage of back discharge. From this stage, the transitions to volume discharge or surface discharge can occur. The volume discharge regime consists of the propagation of a discharge in space toward the cathode which can be superimposed with the propagation of a discharge above the dielectric layer surface. The diagram reveals the conditions for transitions between back discharge regimes.

  18. EXCHANGE RATE REGIMES AND FISCAL PERFORMANCE. DO FIXED EXCHANGE RATE REGIMES GENERATE MORE DISCIPLINE THAN FLEXIBLE ONES?

    Guillermo J. Vuletin

    2004-01-01

    This paper analyzes the influence of exchange rate regimes on fiscal performance, focusing on the difference between fixed and flexible exchange rates. For these ends, a sample of 83 countries for the 1974-1998 period, the GMM methodology for dynamic proposal panel models proposed by Arellano and Bond (1991) and diverse exchange rate classifications are used. In relation to the latter, this paper discusses recent regime classifications and proposes a new exchange rate classification that perm...

  19. Exchange Rate Regimes – A periodical overview and a critical analysis of exchange rate regimes in Kosovo

    Flamur Bunjaku

    2015-01-01

    Exchange rate regimes and the monetary policy are the key instruments governments use to achieve their economic and financial objectives. Moreover, due to global financial crisis the latter instruments get more importance. Empirical evidences show that exchange rate regimes in Kosovo and its monetary policy throughout their development were mainly influenced by different political and historical developments. In regard of Euroisation of monetary system in Kosovo it was found that this action ...

  20. Regime maintenance in post-Soviet Kazakhstan: the case of the regime and oil industry relationship (1991-2005)

    Ostrowski, Wojciech

    2008-01-01

    The main aim of this thesis is to investigate the ways in which the authoritarian regime in post-Soviet Kazakhstan maintained itself in power from 1991 until 2005. This study endeavours to uncover the palette of the regime’s methods by analysing the ways in which it went about controlling the oil industry – an industry with which the political and economical future of Kazakhstan is inseparably intertwined. The empirical section of this study investigates the interplay between the regime and...

  1. Positron acceleration in doughnut wakefields in the blowout regime

    Vieira, Jorge; Mendonca, Jose; Fonseca, Ricardo; Silva, Luis

    2014-10-01

    Most important plasma acceleration results were reached in the so called bubble or blowout regime. Although ideally suited for electron acceleration, it has been recognized that non-linear regimes are not adequate to accelerate positrons. New configurations enabling positron acceleration in non-linear regimes would therefore open new research paths for future plasma based collider configurations. In this work, we explore, analytically and through 3D OSIRIS simulations, a novel configuration for positron acceleration in strongly non-linear laser wakefield excitation regimes using Laguerre-Gaussian laser drivers to drive doughnut shaped wakefields with positron focusing and accelerating fields. We demonstrate that positron focusing-fields can be up to an order of magnitude larger than electron focusing in the spherical blowout regime. The amplitude of the accelerating fields is similar to the spherical blowout. Simulations demonstrate laser self-guiding and stable positron acceleration until the laser energy has been exhausted to the plasma. Other realisations of the scheme, using two Gaussian laser pulses, will also be explored. FCT Grant No EXPL/FIS-PLA/0834/2012 and European Research Council ERC-2010-AdG Grant No. 267841.

  2. THE IMPACT OF THE GLOBAL NUCLEAR SAFETY REGIME IN BRAZIL

    A turning point of the world nuclear industry with respect to safety occurred due to the accident at Chernobyl, in 1986. A side from the tragic personal losses and the enormous financial damage, the Chernobyl accident has literally demonstrated that ''a nuclear accident anywhere is an accident everywhere''. The impact was felt immediately by the nuclear industry, with plant cancellations (e.g. Austria), elimination of national programs (e.g. Italy) and general construction delays. However, the reaction of the nuclear industry was equally immediate, which led to the proposal and establishment of a Global Nuclear Safety Regime. This regime is composed of biding international safety conventions, globally accepted safety standard, and a voluntary peer review system. In a previous work, the author has presented in detail the components of this Regime, and briefly discussed its impact in the Brazilian nuclear power organizations, including the Regulatory Body. This work, on the opposite, briefly reviews the Global Nuclear Safety Regime, and concentrates in detail in the discussion of its impact in Brazil, showing how it has produced some changes, and where the peer pressure regime has failed to produce real results

  3. Electron velocity shear driven instability in relativistic regime

    The electron magnetohydrodynamics model has been generalized to incorporate relativistic effects. The model is then employed to study the instability associated with sheared electron velocity flow in the relativistic regime. The instability has features similar to the conventional velocity shear driven Kelvin-Helmholtz-like mode [A. Das and P. Kaw, Phys. Plasmas 8, 4518 (2001)] in the weakly relativistic regime. However, in the strongly relativistic regime the instability shows certain distinct characteristics. The threshold value of the wave number is found to be considerably higher than the inverse shear width of the equilibrium velocity profile in this regime. Thus, the unstable domain of the wave-number space is considerably wider in this case. Also the mode does not remain purely growing but acquires a real frequency even for an antisymmetric velocity profile. These features of the mode have been understood by realizing that in the strongly relativistic regime the relativistic mass factor γ0 for the equilibrium has much sharper variations than that of the velocity profile.

  4. Early signatures of regime shifts in complex dynamical systems

    Indrani Bose; Mainak Pal

    2015-02-01

    A large number of studies have recently been carried out on the early signatures of regime shifts in a number of dynamical systems, e.g., ecosystems, the climate, fish and wildlife populations, financial markets, complex diseases and gene circuits. The underlying model in most cases is that of the fold-bifurcation in which a sudden regime shift occurs at a bifurcation point. The shift involves a discontinuous jump from one type of stable steady state to another. The dynamics of natural systems have both deterministic and stochastic components. The early signatures of abrupt regime shifts include the critical slowing down as a transition point is approached, rising variance and the lag-1 autocorrelation function, increased skewness of the steady-state probability distribution and the ratio of two mean first passage times for the exits from the stable steady states as the bifurcation point is approached. Noise-induced regime shifts are also possible for which the vicinity of the bifurcation point is not essential. In this paper, we review examples of regime shifts in natural systems and the associated early signatures. We further discuss how such approaches provide useful insights on a cell biological process involving the fold-bifurcation.

  5. Winter thermal regime of the LG 2 reservoir (James Bay)

    During winter, discharge from the stratified La Grande 2 reservoir affects the thermal regime of the downstream reach of the La Grande River, which in turn has repercussions on the hydraulic and ice regimes. The precision and reliability of modern electronic thermometers now permit automated electronically digitized data collection. Such instruments were installed in the large northern reservoir of the La Grande 2 powerplant and its downstream reach, to measure water temperatures during winter. Temperatures were recorded at depths ranging between 2 and 92 m and indicate a behaviour controlled mainly by the ice cover and the flow regime of the discharge at the LG 2 powerplant. Time series analyses performed in both time and frequency domains, provided information on the thermal regimes of the LG 2 reservoir and on the main layer of the flow drawn off by the powerplant. Field measurements from the 1988-89 and 1989-90 winter surveys are presented, and a description is presented of the water temperature regimes of the LG 2 reservoir and the downstream river. 3 refs., 14 figs

  6. 急性高危肺栓塞介入取栓、溶栓中应用 r-tPA 与尿激酶溶栓时间及疗效比较%Comparison of curative effect and thrombolysis time between r-tPA application and urokinase in the interventional thrombectomy and thrombolysis for acute and severe pulmonary embolism

    冯琦琛; 李选; 董国祥; 傅军; 王昌明

    2014-01-01

    Objective:To study the influence of r-tPA and urokinase in curative effect and thrombolysis time on patients with acute and severe pulmonary embolism after interventional thrombectomy .Methods:After reviewing and analyzing the clinical data of 19 acute and severe pulmonary embolism patients , we classified them into two groups in accordance with the application of r-tPA and urokinase to compare the changes of their heart rate , blood pressure , pulmonary arterial pressure , arterial partial pressure of oxygen and hemachrome before and after thrombolysis , as well as the thrombolysis effect time the two groups took.Results: The heart rate, blood pressure, pulmonary arterial pressure , arterial partial pressure of oxygen of the patients in both groups gained remarkable improvement after operation ( P0.05); the pulmonary arterial pressure of the urokinase patient group dropped significantly compared with that study before operation ( P0 .05 );the thrombolysis effect time by adop-ting r-tPA was remarkably shorter than that caused in thrombolysis by adopting urokinase ( P<0 .05 ) . Conclusion: Both r-tPA and urokinase are effective in interventional thrombolysis for acute and severe pulmonary embolism .However , r-tPA could significantly shorten the time caused in thrombolysis without increasing any bleeding risk.%目的:探讨急性高危型肺栓塞患者经介入取栓后,应用r-tPA和尿激酶行介入溶栓治疗,对疗效和溶栓时间的影响。方法:回顾性分析19例急性高危型肺栓塞患者的临床资料,根据其应用r-tPA或尿激酶行介入溶栓分为两组,比较两组间溶栓前后心率、血压、肺动脉压、动脉血氧分压和血红蛋白的变化,以及两组的溶栓时间。结果:各组患者术前和术后心率、血压、动脉血氧分压均有明显改善(P<0.05);应用r-tPA组患者术后肺动脉压较术前有所降低,但差异无统计学意义(P>0.05),应用尿激酶组患者术

  7. Soil magnetic susceptibility reflects soil moisture regimes and the adaptability of tree species to these regimes

    Wang, J.-S.; Grimley, D.A.; Xu, C.; Dawson, J.O.

    2008-01-01

    Flooded, saturated or poorly drained soils are frequently anaerobic, leading to dissolution of the strongly magnetic minerals, magnetite and maghemite, and a corresponding decrease in soil magnetic susceptibility (MS). In this study of five temperate deciduous forests in east-central Illinois, USA, mean surface soil MS was significantly higher adjacent to upland tree species (31 ?? 10-5 SI) than adjacent to floodplain or lowland tree species (17 ?? 10-5 SI), when comparing regional soils with similar parent material of loessal silt. Although the sites differ in average soil MS for each tree species, the relative order of soil MS means for associated tree species at different locations is similar. Lowland tree species, Celtis occidentalis L., Ulmus americana L., Acer saccharinum L., Carya laciniosa (Michx. f.) Loud., and Fraxinus pennsylvanica Marsh. were associated with the lowest measured soil MS mean values overall and at each site. Tree species' flood tolerance rankings increased significantly, as soil MS values declined, the published rankings having significant correlations with soil MS values for the same species groups. The three published classifications of tree species' flood tolerance were significantly correlated with associated soil MS values at all sites, but most strongly at Allerton Park, the site with the widest range of soil drainage classes and MS values. Using soil MS measurements in forests with soil parent material containing similar initial levels of strongly magnetic minerals can provide a simple, rapid and quantitative method to classify soils according to hydric regimes, including dry conditions, and associated plant composition. Soil MS values thus have the capacity to quantify the continuum of hydric tolerances of tree species and guide tree species selection for reforestation. ?? 2007 Elsevier B.V. All rights reserved.

  8. Quantum SASE Regime for X-Ray FEL

    Avetissian, H K

    2005-01-01

    For the X-ray FEL if the amplifying photon energy is larger or comparable to resonance widths caused by an electron beam spreads or by the finite interaction length then the quantum effects may play a significant role [1] and the generation process shifts from the classical to the quantum regime. The recent advancement of high brightness particle and laser beams technology makes achievable the fulfillment of these conditions in the scheme of X-ray Backscatter Compton laser. In this work we consider in general the SASE X-ray FEL in the quantum regime of amplification arising from the self-consistent set of the Maxwell and relativistic quantum kinetic equations. It is shown a considerable increase in start-up and narrowing of the spectrum of the SASE power for amplifying X-ray frequencies compared with the classical SASE regime.

  9. Dynamo regimes and transitions in the VKS experiment

    Berhanu, M; Boisson, J; Gallet, B; Gissinger, C; Fauve, S; Mordant, N; Pétrélis, F; Bourgoin, M; Odier, P; Pinton, J -F; Plihon, N; Aumaître, S; Chiffaudel, A; Daviaud, F; Dubrulle, B; Pirat, C

    2015-01-01

    The Von K{\\'a}rm{\\'a}n Sodium experiment yields a variety of dynamo regimes, when asymmetry is imparted to the flow by rotating impellers at different speed F1 and F2. We show that as the intensity of forcing, measured as F1 + F2, is increased, the transition to a self-sustained magnetic field is always observed via a supercritical bifurcation to a stationary state. For some values of the asymmetry parameter $\\\\theta$ = (F1--F2)/(F1+F2), time dependent dynamo regimes develop. They are observed either when the forcing is increased for a given value of asymmetry, or when the amount of asymmetry is varied at sufficiently high forcing. Two qualitatively different transitions between oscillatory and stationary regimes are reported, involving or not a strong divergence of the period of oscillations. These transitions can be interpreted using a low dimensional model based on the interactions of two dynamo modes.

  10. Adelie penguin foraging location predicted by tidal regime switching.

    Matthew J Oliver

    Full Text Available Penguin foraging and breeding success depend on broad-scale environmental and local-scale hydrographic features of their habitat. We investigated the effect of local tidal currents on a population of Adélie penguins on Humble Is., Antarctica. We used satellite-tagged penguins, an autonomous underwater vehicle, and historical tidal records to model of penguin foraging locations over ten seasons. The bearing of tidal currents did not oscillate daily, but rather between diurnal and semidiurnal tidal regimes. Adélie penguins foraging locations changed in response to tidal regime switching, and not to daily tidal patterns. The hydrography and foraging patterns of Adélie penguins during these switching tidal regimes suggest that they are responding to changing prey availability, as they are concentrated and dispersed in nearby Palmer Deep by variable tidal forcing on weekly timescales, providing a link between local currents and the ecology of this predator.

  11. Supercurrent in the quantum Hall regime, part II

    Amet, Francois; Ke, Chung Ting; Borzenets, Ivan; Wang, Jiyingmei; Watanabe, Kenji; Taniguchi, Takashi; Deacon, Russel; Yamamoto, Michihisa; Bomze, Yuriy; Tarucha, Seigo; Finkelstein, Gleb

    A novel promising route for creating topological states and excitations is to combine superconductivity and the quantum Hall effect. Despite this potential, signatures of superconductivity in the quantum Hall regime remain scarce, and a superconducting current through a Landau-quantized two-dimensional electron gas has so far eluded experimental observation. High-mobility graphene/BN heterostructures exhibit the quantum Hall effect at relatively low field and are therefore particularly suitable to study the fate of the Josephson effect in that regime. Here, we report the observation of a superconducting current through graphene at fields as high as 2 Tesla. In that regime, the normal-state resistance is quantized but pockets of superconductivity still persist at small current bias. We will describe their bias and temperature dependence. Magnetic field interference patterns in the supercurrent inform on possible mechanisms mediating this supercurrent.

  12. A Markov Switching Regime Model of Malaysia Property Cycle

    Abdul M. Beksin

    2011-01-01

    Full Text Available Problem statement: Non-linear models such as the Markov Switching regime (MS method of modelling business cycles, in principle can be used to model property cyle. Approach: The MS model can distinguish property cycle in recession and expansion phases and is sufficiently flexible to allow different relationships to apply over these phases. In this study, the Malaysian property cycle is modelled using a MS model. Results: This technique can be used to simultaneously estimate the data generating process of real GDP growth and classify each observation into one of two regimes (i.e., low-growth and high-growth regimes. Conclusions: This finding has important policy implications, since the yield spread was used to generate the time-varying probabilities of the MS model as well as the recession probabilities of the logit model. In other words, a strong relationship exists between interest rates and the business cycle, where interest rates lead the business cycle.

  13. Dispersive regime of the Jaynes–Cummings and Rabi lattice

    Photon-based strongly correlated lattice models like the Jaynes–Cummings and Rabi lattices differ from their more conventional relatives like the Bose–Hubbard model by the presence of an additional tunable parameter: the frequency detuning between the pseudo-spin degree of freedom and the harmonic mode frequency on each site. Whenever this detuning is large compared to relevant coupling strengths, the system is said to be in the dispersive regime. The physics of this regime is well-understood at the level of a single Jaynes–Cummings or Rabi site. Here, we extend the theoretical description of the dispersive regime to lattices with many sites, for both strong and ultra-strong coupling. We discuss the nature and spatial range of the resulting qubit–qubit and photon–photon coupling, demonstrate the emergence of photon-pairing and squeezing and illustrate our results by exact diagonalization of the Rabi dimer. (paper)

  14. The difficulties of the Chinese and Indian exchange rate regimes

    Ila Patnaik

    2009-06-01

    Full Text Available China and India have both sought control over the exchange rate in order to maintain export competitiveness, manage current account balance, and pursue independent monetary policy. In this paper, we examine structural change in the Chinese and Indian de facto exchange rate regimes, focusing on the period from 1998 to 2007. With increasing capital account openness, exchange rate inflexibility has been associated with significant monetary policy distortions. In both countries, the short-term rate expressed in real terms dropped, and achieved very low values, in the unprecedented business cycle expansion of the early 2000s. In the Indian case, difficulties of sterilisation led to a modification of the exchange rate regime, moving towards greater flexibility. In China, in contrast, the exchange rate regime did not change.

  15. Oil Regime Change in Iraq. Possible Strategic Implications for OPEC

    The potential strategic impact of regime change in Iraq and Iran on OPEC in the long-run is explored. In the first part of the paper short overviews are given of the present international oil market; of US oil import issues and energy policy; of the strategic position of the US in the Persian Gulf and of geopolitical developments in the Persian Gulf at large. Also, attention is paid to the OPEC and the role of a 'new' Iraq. In the second part the game of 'boxed pigs' is used to explore the possible strategic impact of regime change in Iraq and possible regime change in Iran on OPEC. This exploration takes place within four possible futures for the Gulf

  16. Temporal evolution of flow regimes in urbanizing basins

    Mejia, A.; Rossel, F.; Gironas, J. A.; Jovanovic, T.

    2014-12-01

    We characterize the temporal evolution of the flow regime of urbanizing basins. By urbanizing basins, we mean basins that have experienced urban growth during their observation period. To represent the flow regime, we use flow duration curves (FDCs). We compute the FDCs using a stochastic model of daily streamflow for urban basins. In this case, the model aids in discerning the influence of key factors (e.g., climate, land use change, stormwater managenment conditions, and the slow and fast properties of the hydrologic response) on streamflow. To implement the model, we first divide the complete observation period of a given urban basin into intervals of equal duration, e.g. 5 years. Subsequently, we apply the model to each interval and this is how we capture the influence of land use changes and climatic fluctuations on the flow regime. We apply this modeling framework to 14 urbanizing basins in the Baltimore-Washington DC region. Results from this application indicate consistent changes in the temporal evolution of the altered flow regimes, which can largely be explained by the progressive redistribution with urban growth of water from slow subsurface runoff and evapotranspiration to fast urban runoff. We also use the modeling framework to determine indicators of ecohydrological alteration for urbanizing basins. The application of these indicators to our study area suggests that the flow regime is sensitive to alterations up to a certain level of urbanization after which sensitivity seems to level off. The flow regime also seems to be relatively more resistant to alterations for both the smaller and larger levels of urbanization considered. In the future, we would like to extend the application of the proposed modeling framework to other metropolitan areas.

  17. Modeling whistler wave generation regimes in magnetospheric cyclotron maser

    D. L. Pasmanik

    2004-11-01

    Full Text Available Numerical analysis of the model for cyclotron instability in the Earth's magnetosphere is performed. This model, based on the self-consistent set of equations of quasi-linear plasma theory, describes different regimes of wave generation and related energetic particle precipitation. As the source of free energy the injection of energetic electrons with transverse anisotropic distribution function to the interaction region is considered. A parametric study of the model is performed. The main attention is paid to the analysis of generation regimes for different characteristics of energetic electron source, such as the shape of pitch angle distributions and its intensity. Two mechanisms of removal of energetic electrons from a generation region are considered, one is due to the particle precipitation through the loss cone and another one is related to the magnetic drift of energetic particles.

    It was confirmed that two main regimes occur in this system in the presence of a constant particle source, in the case of precipitation losses. At small source intensity relaxation oscillations were found, whose parameters are in good agreement with simplified analytical theory developed earlier. At a larger source intensity, transition to a periodic generation occurs. In the case of drift losses the regime of self-sustained periodic generation regime is realized for source intensity higher than some threshold. The dependencies of repetition period and dynamic spectrum shape on the source parameters were studied in detail. In addition to simple periodic regimes, those with more complex spectral forms were found. In particular, alteration of spikes with different spectral shape can take place. It was also shown that quasi-stationary generation at the low-frequency band can coexist with periodic modulation at higher frequencies.

    On the basis of the results obtained, the model for explanation of

  18. Strong and moderate nonlinear El Niño regimes

    Takahashi, Ken; Dewitte, Boris

    2016-03-01

    It has been previously proposed that two El Niño (EN) regimes, strong and moderate, exist but the historical observational record is too short to establish this conclusively. Here, 1200 years of simulations with the GFDL CM2.1 model allowed us to demonstrate their existence in this model and, by showing that the relevant dynamics are also evident in observations, we present a stronger case for their existence in nature. In CM2.1, the robust bimodal probability distribution of equatorial Pacific sea surface temperature (SST) indices during EN peaks provides evidence for the existence of the regimes, which is also supported by a cluster analysis of these same indices. The observations agree with this distribution, with the EN of 1982-1983 and 1997-1998 corresponding to the strong EN regime and all the other observed EN to the moderate regime. The temporal evolution of various indices during the observed strong EN agrees very well with the events in CM2.1, providing further validation of this model as a proxy for nature. The two regimes differ strongly in the magnitude of the eastern Pacific warming but not much in the central Pacific. Observations and model agree in the existence of a finite positive threshold in the SST anomaly above which the zonal wind response to warming is strongly enhanced. Such nonlinearity in the Bjerknes feedback, which increases the growth rate of EN events if they reach sufficiently large amplitude, is very likely the essential mechanism that gives rise to the existence of the two EN regimes. Oceanic nonlinear advection does not appear essential for the onset of strong EN. The threshold nonlinearity could make the EN regimes very sensitive to stochastic forcing. Observations and model agree that the westerly wind stress anomaly in the central equatorial Pacific in late boreal summer has a substantial role determining the EN regime in the following winter and it is suggested that a stochastic component at this time was key for the

  19. Focal shift of silicon microlens in mid-infrared regime

    Zuo, Haijie; Ying, Leiying; Zhang, Baoping; Hou, Zhijin; Chen, Hongxu; Si, Junjie

    2016-01-01

    In this study, rigorous numerical calculation was utilized to characterize the focal properties of mid-infrared silicon microlens with the size about tens of micrometers. It is found that the focal shift phenomenon also exists in mid-infrared regime, which behaves differently from that of visual and near-infrared wavelength. Focal properties of silicon microlens were also measured experimentally, showing well coherence with simulation results. Our results provide systemic understanding of focal shift in mid-infrared regime, at that wavelength special consideration should be paid in micro-nano optics, especially with the integration between infrared optical system and other devices.

  20. NAZI REGIME IN THE PERIODICAL A NOTÍ­CIA.

    Bruna Luíza Barcellos

    2009-08-01

    Full Text Available The periodical A Notícia, of Joinville, city colonized for Germans, supported the nazi regime since the ascension of Adolf Hitler to the power. Between 1932 and 1944, some editions had printed the figure of Hitler and the nazi symbol, beyond dither messages to that regime. Based in the Análise de conteúdo (BARDIN, 1977, this article searchs to understand the representation made for the periodical A Notícia on this historical landmark, in other words, bring to the light the consequences of a part of occured world-wide history in catarinense territory.

  1. Development and current trends in the international nonproliferation regime

    The IAEA and its safeguards system is placed into a historical perspective. A personal perspective on the nonproliferation regime and on the Agency's role in it is then presented. This is done initially by discussing some of the landmark events in the history of the nonproliferation regime. Subsequently some of the history of arms control agreements and of the role of the IAEA are noted. Then political motivations of state and ways the Agency has an impact in the political nonproliferation sphere are addressed

  2. Using Clustering to Establish Climate Regimes from PCM Output

    Oglesby, Robert; Arnold, James E. (Technical Monitor); Hoffman, Forrest; Hargrove, W. W.; Erickson, D.

    2002-01-01

    A multivariate statistical clustering technique--based on the k-means algorithm of Hartigan has been used to extract patterns of climatological significance from 200 years of general circulation model (GCM) output. Originally developed and implemented on a Beowulf-style parallel computer constructed by Hoffman and Hargrove from surplus commodity desktop PCs, the high performance parallel clustering algorithm was previously applied to the derivation of ecoregions from map stacks of 9 and 25 geophysical conditions or variables for the conterminous U.S. at a resolution of 1 sq km. Now applied both across space and through time, the clustering technique yields temporally-varying climate regimes predicted by transient runs of the Parallel Climate Model (PCM). Using a business-as-usual (BAU) scenario and clustering four fields of significance to the global water cycle (surface temperature, precipitation, soil moisture, and snow depth) from 1871 through 2098, the authors' analysis shows an increase in spatial area occupied by the cluster or climate regime which typifies desert regions (i.e., an increase in desertification) and a decrease in the spatial area occupied by the climate regime typifying winter-time high latitude perma-frost regions. The patterns of cluster changes have been analyzed to understand the predicted variability in the water cycle on global and continental scales. In addition, representative climate regimes were determined by taking three 10-year averages of the fields 100 years apart for northern hemisphere winter (December, January, and February) and summer (June, July, and August). The result is global maps of typical seasonal climate regimes for 100 years in the past, for the present, and for 100 years into the future. Using three-dimensional data or phase space representations of these climate regimes (i.e., the cluster centroids), the authors demonstrate the portion of this phase space occupied by the land surface at all points in space and time

  3. Terahertz Quantum Plasmonics of Nanoslot Antennas in Nonlinear Regime.

    Kim, Joon-Yeon; Kang, Bong Joo; Park, Joohyun; Bahk, Young-Mi; Kim, Won Tae; Rhie, Jiyeah; Jeon, Hyeongtag; Rotermund, Fabian; Kim, Dai-Sik

    2015-10-14

    Quantum tunneling in plasmonic nanostructures has presented an interesting aspect of incorporating quantum mechanics into classical optics. However, the study has been limited to the subnanometer gap regime. Here, we newly extend quantum plasmonics to gap widths well over 1 nm by taking advantage of the low-frequency terahertz regime. Enhanced electric fields of up to 5 V/nm induce tunneling of electrons in different arrays of ring-shaped nanoslot antennas of gap widths from 1.5 to 10 nm, which lead to a significant nonlinear transmission decrease. These observations are consistent with theoretical calculations considering terahertz-funneling-induced electron tunneling across the gap. PMID:26372787

  4. Switching Monetary Policy Regimes and the Nominal Term Structure

    Ferman, Marcelo

    2011-01-01

    In this paper I propose a regime-switching approach to explain why the U.S. nominal yield curve on average has been steeper since the mid-1980s than during the Great Inflation of the 1970s. I show that, once the possibility of regime switches in the short-rate process is incorporated into investors' beliefs, the average slope of the yield curve generally will contain a new component called 'level risk'. Level-risk estimates, based on a Markov-Switching VAR model of the U.S. economy, are then ...

  5. Measurement of flow in supercritical flow regime using cutthroat flumes

    Shrikant A Tekade; Avinash D Vasudeo; Aniruddha D Ghare; Ramesh N Ingle

    2016-02-01

    Cutthroat flume is commonly used for measurement of subcritical flow in open channel because of its simplicity and ease of construction. No experimental data is available in literature for measurement of flow in supercritical regime using cutthroat flume. The present paper finds the feasibility of cutthroat flume as a measurement device for flow in supercritical regime. Experimental data are generated to develop the relation between discharge and observed head at a specified location on upstream of throat section. Regression analysis for discharge and head indicated a good correlation. Based on all the experimental data generated, a relationship between discharge and head is proposed.

  6. Water regime and core state in WWER reactors

    Some features of the ammonia-potassium water regime used in the WWER-reactors are considered. This regime makes it possible to suppress water radiolysis and simultaneously obtain a high pH value of the coolant, which is necessary in operation with boron control of the reactor power. Absence of oxygen and significant deposits on the zirconium alloy claddings of the fuel elements affect favourably the corrosion condition of the core. The thickness of the corrosion film on the fuel cladding at the end of their lifetime is as low as 3μm and the hydrogen content remains at the initial level

  7. Explicit Mapping of Acoustic Regimes For Wind Instruments

    Missoum, Samy; Doc, Jean-Baptiste

    2014-01-01

    This paper proposes a methodology to map the various acoustic regimes of wind instruments. The maps can be generated in a multi-dimensional space consisting of design, control parameters, and initial conditions. The bound- aries of the maps are obtained explicitly in terms of the parameters using a support vector machine (SVM) classifier as well as a dedicated adaptive sam- pling scheme. The approach is demonstrated on a simplified clarinet model for which several maps are generated based on different criteria. Examples of computation of the probability of occurrence of a specific acoustic regime are also provided. In addition, the approach is demonstrated on a design optimization example for optimal intonation.

  8. The French regime of civil liability for nuclear

    As civil liability for nuclear is a matter of discussion and initiatives at the European and international levels, the author proposes an overview of the legal framework of the French regime of civil liability for nuclear which is a combination of two international treaties (Paris and Brussels conventions) and a national arrangement (a 1968 law). He presents and comments the main characteristics of this regime (geographical scope of application, concerned activities, excluded events, covered damages, principles regarding operator's liability) and the improvements brought by Paris and Brussels convention review protocols

  9. Explicit mapping of acoustic regimes for wind instruments

    Missoum, Samy; Vergez, Christophe; Doc, Jean-Baptiste

    2014-09-01

    This paper proposes a methodology to map the various acoustic regimes of wind instruments. The maps can be generated in a multidimensional space consisting of design, control parameters, and initial conditions. The boundaries of the maps are obtained explicitly in terms of the parameters using a Support Vector Machine (SVM) classifier as well as a dedicated adaptive sampling scheme. The approach is demonstrated on a simplified clarinet model for which several maps are generated based on different criteria. Examples of computation of the probability of occurrence of a specific acoustic regime are also provided. In addition, the approach is demonstrated on a design optimization example for optimal intonation.

  10. PREDICTION OF FLOW REGIMES IN SPOUT-FLUIDIZED BEDS

    Jiyu Zhang; Fengxiang Tang

    2006-01-01

    Five main flow regimes in spout-fluidized bed were identified in this study, namely, fixed bed, spout with aeration, spout-fluidization, jet in fluidized bed and slugging, together with their corresponding major frequencies translated from pressure signals. The empirical equation A=aBb, in which A=Fr* /(H/Di) and B=(Fr*/(H/D))/(μg/μmf) are respectively the spout-geometry and spout-geometry-fluidization dimensionless numbers, was proposed to distinguish these flow regimes.

  11. Electron Vacuum Acceleration in a Regime beyond Brunel Absorption

    We describe a new regime of electron acceleration in laser plasmas driven by ultrafast pulses of relativistic intensity, in which space-charge separation leads to strongly enhanced laser absorption and the production of 20 MeV (p/m0c≅40) electrons driven outward in vacuum. 1D PIC simulations show that intense attosecond pulses generated around critical density can sweep electrons outward over many wavelengths in distance. With increasing interaction scale length, absorption generalizes from the Brunel regime to one in which absorption is primarily into electrons of energy >>5 MeV.

  12. Non-linear regimes in resistive MHD equations

    The resistive MHD equations in ''slab'' geometry and helical symmetry are considered in a dissipative dynamical system approach with the aim to elucidate the basic mechanisms for the non-linear regimes in plasmas. One-dimensional equilibria are found to be destabilised via symmetry breaking bifurcation to stationary solutions with ''island-vortex'' structures. Conditions are discussed for which further destabilisation to time-dependent regimes at higher Reynolds number leads to ''sawtooth-like'' oscillations. The ''crash'' in magnetic energy is found to be due to a fast drop for the one-dimensional magnetic field connected with enhancement of ''island-vortex'' activity. (Author)

  13. 尿激酶不同给药方法治疗动静脉内瘘血栓的疗效观察%Urokinase different medication for the treatment of thecurative effect of venous fistula thrombosis observation

    毛艳华

    2014-01-01

    Objective To observe the urokinase two differentmedication for the treatment of hemodialysisarteriovenous fistula in patients receiving thrombolyticeffect. Methods In our hospital from 2010 October to 2013October autologous artery fistula thrombosis in 26 cases of intravenous, were randomly divided into two groups.Group A:continuous micro pump infusion of thrombolyticgroup;group B:intermittent intravenous thrombolysis group. Results The effect of the two groups were compared with A group of thrombolysis in 11 cases,accounting for 84.6%;group B thrombolysis in 8 cases,accounting for 61.5%. Continuous micro injection pump is better than that of treatment with intermittent intravenousinjection group. The difference between groups was statistically significant at P<0.05. Conclusion Urokinasethrombolysis continued micro pump injection lasting effect,its efficacy and thromboembolism is closely related toearly thrombolytic effect.%目的:观察尿激酶两种不同的给药方法治疗血液透析患者自体动静脉内瘘血栓溶栓的疗效。方法选取我院2010年10月-2013年10月自体动静脉内瘘血栓形成患者26例,随机分为两组。 A组:连续性微泵注射溶栓组;B组:间断静脉注射溶栓组。结果比较两组溶栓的效果A组溶通11例,占84.6%;B组溶通8例,占61.5%。连续性微泵注射溶栓治疗效果优于间断静脉注射组。组间比较差异有统计学意义P<0.05。结论尿激酶持续微泵注射溶栓作用持久,其疗效与血栓栓塞时间密切相关,早期溶栓效果显著。

  14. Combined mRNA expression levels of members of the urokinase plasminogen activator (uPA) system correlate with disease-associated survival of soft-tissue sarcoma patients

    Members of the urokinase-type plasminogen activator (uPA) system are up-regulated in various solid malignant tumors. High antigen levels of uPA, its inhibitor PAI-1 and its receptor uPAR have recently been shown to be associated with poor prognosis in soft-tissue sarcoma (STS) patients. However, the mRNA expression of uPA system components has not yet been comprehensively investigated in STS patients. The mRNA expression level of uPA, PAI-1, uPAR and an uPAR splice variant, uPAR-del4/5, was analyzed in tumor tissue from 78 STS patients by quantitative PCR. Elevated mRNA expression levels of PAI-1 and uPAR-del4/5 were significantly associated with clinical parameters such as histological subtype (P = 0.037 and P < 0.001, respectively) and higher tumor grade (P = 0.017 and P = 0.003, respectively). In addition, high uPAR-del4/5 mRNA values were significantly related to higher tumor stage of STS patients (P = 0.031). On the other hand, mRNA expression of uPA system components was not significantly associated with patients' survival. However, in STS patients with complete tumor resection (R0), high PAI-1 and uPAR-del4/5 mRNA levels were associated with a distinctly increased risk of tumor-related death (RR = 6.55, P = 0.054 and RR = 6.00, P = 0.088, respectively). Strikingly, R0 patients with both high PAI-1 and uPAR-del4/5 mRNA expression levels showed a significant, 19-fold increased risk of tumor-related death (P = 0.044) compared to the low expression group. Our results suggest that PAI-1 and uPAR-del4/5 mRNA levels may add prognostic information in STS patients with R0 status and distinguish a subgroup of R0 patients with low PAI-1 and/or low uPAR-del4/5 values who have a better outcome compared to patients with high marker levels

  15. Process-based humidity control regime for greenhouse crops

    Korner, O.; Challa, H.

    2003-01-01

    Modern greenhouses in The Netherlands are designed for efficient use of energy. Climate control traditionally aims at optimal crop performance. However, energy saving is a major issue for the development of new temperature regimes. Temperature integration (TI) results in fluctuating and often high r

  16. Analysis of the Two-Regime Method on Square Meshes

    Flegg, Mark B.

    2014-01-01

    The two-regime method (TRM) has been recently developed for optimizing stochastic reaction-diffusion simulations [M. Flegg, J. Chapman, and R. Erban, J. Roy. Soc. Interface, 9 (2012), pp. 859-868]. It is a multiscale (hybrid) algorithm which uses stochastic reaction-diffusion models with different levels of detail in different parts of the computational domain. The coupling condition on the interface between different modeling regimes of the TRM was previously derived for onedimensional models. In this paper, the TRM is generalized to higher dimensional reaction-diffusion systems. Coupling Brownian dynamics models with compartment-based models on regular (square) two-dimensional lattices is studied in detail. In this case, the interface between different modeling regimes contains either flat parts or right-angle corners. Both cases are studied in the paper. For flat interfaces, it is shown that the one-dimensional theory can be used along the line perpendicular to the TRM interface. In the direction tangential to the interface, two choices of the TRM parameters are presented. Their applicability depends on the compartment size and the time step used in the molecular-based regime. The two-dimensional generalization of the TRM is also discussed in the case of corners. © 2014 Society for Industrial and Applied Mathematics.

  17. Search Regimes and the Industrial Dynamics of Science

    Bonaccorsi, Andrea

    2008-01-01

    The article addresses the issue of dynamics of science, in particular of new sciences born in twentieth century and developed after the Second World War (information science, materials science, life science). The article develops the notion of search regime as an abstract characterization of dynamic patterns, based on three dimensions: the rate of…

  18. Syria: The Consolidation of the Asad Regime, 1970-1975.

    Olson, Robert W.

    1982-01-01

    Up to 1975-76, Syria's Hafiz al-Asad was successful in consolidating his power, securing the maintenance of his regime, and broadening his base of political power. His system started weakening in 1975 due to increased opposition stemming from Syria's intervention in the Lebanese civil war. (AM)

  19. Just for Fun? The Emotional Regime of Experiential Consumption

    Jantzen, Christian; Fitchett, James; Østergaard, Per;

    2012-01-01

    Experiential consumption emphasizes emotional and hedonic qualities in the marketplace stressing the importance of experiences for ‘the good life’ and positioning consumption as a legitimate way to generate interesting and relevant experiences. The concept of emotional regimes (Reddy, 2001) is us...... the sake of self-actualization....

  20. Understanding shifts in wildfire regimes as emergent threshold phenomena.

    Zinck, Richard D; Pascual, Mercedes; Grimm, Volker

    2011-12-01

    Ecosystems driven by wildfire regimes are characterized by fire size distributions resembling power laws. Existing models produce power laws, but their predicted exponents are too high and fail to capture the exponent's variation with geographic region. Here we present a minimal model of fire dynamics that describes fire spread as a stochastic birth-death process, analogous to stochastic population growth or disease spread and incorporating memory effects from previous fires. The model reproduces multiple regional patterns in fire regimes and allows us to classify different regions in terms of their proximity to a critical threshold. Transitions across this critical threshold imply abrupt and pronounced increases in average fire size. The model predicts that large regions in Canada are currently close to this transition and might be driven beyond the threshold in the future. We illustrate this point by analyzing the time series for large fires (>199 ha) from the Canadian Boreal Plains, found to have shifted from a subcritical regime to a critical regime in the recent past. By contrast to its predecessor, the model also suggests that a critical transition, and not self-organized criticality, underlies forest fire dynamics, with implications for other ecological systems exhibiting power-law-like patterns, in particular for their sensitivity to environmental change and control efforts. PMID:22089877