WorldWideScience
 
 
1

Behavior of 32P, 35S, 36Cl and 42K in magnesium oxide  

International Nuclear Information System (INIS)

A separation method of 32P from 35S using magnesium oxide as adsorbent of radiophosphorus is described. The behaviour of 32P and 35S, both carrier-free, on magnesium oxide, individually, in dependence of the amount of the adsorbent, of mixing time, of the pH of the loading solution and of potassium chloride concentration, is studied. The separation of the mentioned radioisotopes, using a misture of them, is also analysed. In order to apply this method to the routine production of carrier-free 35S by potassium chloride irradiation, the adsorption behaviour of the chloride and potassium on magnesium oxide using radioactive tracers of these elements, is studied. The separation of 35S from 32P is analyzed by the maximum range of ?- particles in aluminum. The absorption curves are presented and compared. (Author)

1979-01-01

2

Infection of neuroblastoma cells with Semliki Forest virus. Incorporation of 35S or 32P  

International Nuclear Information System (INIS)

Phosphate-free medium is used for the incorporation of 32P and methionine-free medium for 35S-methionine labelling. After virus replication, the culture shows a clear CPE all of the cells appearing round and dead. Materials used are presented and experimental procedure is described

1976-01-01

3

Effect of graft promoting agent on healing process of graft union, absorption, transportation of 32P and 14C  

International Nuclear Information System (INIS)

[en] Jinchun No.4 cucumber graft seedlings (the rootstock was China squash of black seeds) were used to study the effects of graft promoting agent on healing process of graft union, survival rate, absorption, transportation and distribution of 32P induced from roots and 14C from leaves. The results showed that the graft promoting agent accelerated the healing tissue production, connection of vessel and sieve tube, increased the survival rate, total absorption of 32P, total transportation and ratio of 32P and 14C. The agent caused 32P to reach scion from rootstocks and 14C to reach rootstocks from scion 2 days earlier than control

1999-01-01

4

Seedling growth and 32P, 14C distributed in tillers of hybrid rice  

International Nuclear Information System (INIS)

Hybrid rice D-You-63, D-You-64, Ging-YouZhong, Xie-You-64, Wei-You-64, Wei-You-35 and Hua-You-63 have more tillering capability and tiller number over their parents and CK variety 'Shuang-Gui-36' with positive superdominance heterosis (hp) respectively, but L301Ax R29 has less tillers than the sterile line. Between tiller number and 32P distributed (%) in tillers of Ging-You-Zhong, Xie-You-64, Wei-You-64, Wei-You-35, Hua-Yor-63 and D-You-63 positive correlation has shown except D-You-64 and L301A x R29. Negative correlation is between tiller number and 14C distributed (%) in tillers of Ging-YouZhong, Wei-You-64, Wei-You-35, Hua-You-63 without D-You-63. D-You-64 and Xie-You-63. Seedling plant dry weight of D-You-63, D-You-64, Xie-You-64 and Hua-You-63 have exceeded to their parent and CK variety. In seedling plant height Ging-You-Zhong, Xie-You-64, Hua-You-63, D-You-64 have higher than their parents and CK variety. The relationship between the grain yield and seedling plant vigor has been discussed in this paper.

1988-01-01

5

Relationship between grain yield and 32P, 14C distribution in tiller and panicle of F1 hybrid rice  

International Nuclear Information System (INIS)

Relationship between grain yield and 32P, 14C distribution in tillers and panicles of F1 hybrids and their parents was studied under greenhouse condition through tracing methods with materials of 56 F1 hybrids crossed by various cytoplasmic male sterile lines (CMSL) and their parents. The CMSL were WA, HL, DI, DA, B and Sinica rice hybrids type. The results showed that in F1 hybrids with high grain yield (HGY) 32P and 14C were concentrated in its tillers and panicles at milky stage, in which the 14C-glucose metabolite percentage exported from flag leaf to panicle of F1 was rather high. A close relation was observed between grain yield and 14C-glucose assimilate distributed in panicle, and IAP (14C exported (%) x 14C accumulated in panicle (%)/100). But this feature was not showed in some F1 rice hybrids with low grain yield (LGY). The tracers of 32P, 14C distributed in tillers and panicles can be used to predict heterosis in grain yield of F1 rice hybrids.

1992-01-01

6

A field study of the uptake of 35S and 14C into crops characteristic of the UK diet  

International Nuclear Information System (INIS)

The uptake of 35S and 14C into crops characteristic of the UK diet was studied. Four common types of green vegetable, six common types of root vegetable and perennial ryegrass were grown in a garden plot in the environs of Hinkley Point Nuclear Power Station and the 35S and 14C contents of the crops were measured. Also measured were the corresponding air concentrations over the plot averaged over a range of time periods between sowing and harvesting. The results were analysed in terms of air to crop transfer factors for 35S and 14C and the implications of these for dose calculations were assessed for both collective dose and for a hypothetical critical group consuming a range of foods produced in situ. (author).

1986-01-01

7

Metabolism of 35S- and 14C-labeled 1-methyl-2-mercaptoimidazole in vitro and in vivo  

International Nuclear Information System (INIS)

We previously described an in vitro incubation system for studying the mechanism of inhibition of thyroid peroxidase (TPO)-catalyzed iodination by the antithyroid drug 1-methyl-2-mercaptoimidazole (MMI). Inhibition of iodination in this system may be reversible or irreversible, depending on the relative concentrations of iodide and MMI and on the TPO concentration. Metabolism of the drug occurs under both conditions, and in the present investigation we used 35S- and 14C-labeled MMI together with reverse phase HPLC to examine the metabolic products associated with reversible and irreversible inhibition of iodination by MMI. Under conditions of reversible inhibition, MMI was rapidly metabolized and disappeared completely from the incubation mixture. With [35S]MMI, the earliest detectable 35S-labeled product was MMI disulfide, which reached a peak after a few minutes and then declined to undetectable levels. Coincident with the decrease in disulfide was the appearance of two 35S peaks, the major one corresponding to sulfate/sulfite, and the other to a component eluting at 7.5 min. Similar results were obtained for the disulfide and for the 7.5 min metabolite with [14C]MMI. The major 14C-labeled metabolite containing no S appeared to be 1-methylimidazole. Under conditions of irreversible inhibition, MMI disulfide was also the earliest detectable 35S-labeled metabolite. However, MMI decreased more slowly, and after reaching a nadir at about 6 min returned gradually to a level about halfway between the initial and the minimum value. The reformation of MMI appeared to involve the nonenzymatic disproportionation of MMI disulfide. Formation of the 7.5 min peak was also observed, but there was no formation of sulfate/sulfite

1989-01-01

8

Metabolism of /sup 35/S- and /sup 14/C-labeled 1-methyl-2-mercaptoimidazole in vitro and in vivo  

Energy Technology Data Exchange (ETDEWEB)

We previously described an in vitro incubation system for studying the mechanism of inhibition of thyroid peroxidase (TPO)-catalyzed iodination by the antithyroid drug 1-methyl-2-mercaptoimidazole (MMI). Inhibition of iodination in this system may be reversible or irreversible, depending on the relative concentrations of iodide and MMI and on the TPO concentration. Metabolism of the drug occurs under both conditions, and in the present investigation we used 35S- and 14C-labeled MMI together with reverse phase HPLC to examine the metabolic products associated with reversible and irreversible inhibition of iodination by MMI. Under conditions of reversible inhibition, MMI was rapidly metabolized and disappeared completely from the incubation mixture. With (35S)MMI, the earliest detectable 35S-labeled product was MMI disulfide, which reached a peak after a few minutes and then declined to undetectable levels. Coincident with the decrease in disulfide was the appearance of two 35S peaks, the major one corresponding to sulfate/sulfite, and the other to a component eluting at 7.5 min. Similar results were obtained for the disulfide and for the 7.5 min metabolite with (14C)MMI. The major 14C-labeled metabolite containing no S appeared to be 1-methylimidazole. Under conditions of irreversible inhibition, MMI disulfide was also the earliest detectable 35S-labeled metabolite. However, MMI decreased more slowly, and after reaching a nadir at about 6 min returned gradually to a level about halfway between the initial and the minimum value. The reformation of MMI appeared to involve the nonenzymatic disproportionation of MMI disulfide. Formation of the 7.5 min peak was also observed, but there was no formation of sulfate/sulfite.

Taurog, A.; Dorris, M.L.; Guziec, F.S. Jr.

1989-01-01

9

Metabolism of 35S- and 14C-labeled propylthiouracil in a model in vitro system containing thyroid peroxidase  

International Nuclear Information System (INIS)

In previous communications we described an in vitro model system containing highly purified thyroid peroxidase (TPO) for studying the mechanism of inhibition of thyroid hormone biosynthesis by the antithyroid drugs, 6-propylthiouracil (PTU) and 1-methyl-2-mercaptoimidazole (MMI). We showed that inhibition of iodination of thyroglobulin in this system may be reversible or irreversible depending on the relative concentrations of iodide and drug and the TPO concentration. Metabolism of the drugs occurred under both conditions, but was more limited under irreversible conditions of inhibition. It was of interest to examine the nature of the drug metabolites associated with reversible and irreversible conditions of inhibition. For this purpose we have employed the 35S- and 14C-labeled drugs and a recently developed reverse phase HPLC procedure. Results of a similar study with MMI were reported in an earlier communication. In the present study we report our findings with PTU. Under conditions of reversible inhibition, PTU was readily metabolized and by 15 min was reduced to a few percent of the starting value. The earliest detectable metabolite with both [35S]- and [14C]PTU was the disulfide, which reached a peak in about 15 min and then slowly declined. Coincident with the decline in the disulfide was the appearance of more polar metabolites. In the case of [35S]PTU, these corresponded to sulfate/sulfite, PTU sulfonate, and a product tentatively identified as PTU sulfinate. The latter two were also observed as 14C-labeled metabolites produced from [14C]PTU. Two nonpolar desulfurated 14C-labeled metabolites were also observed. Surprisingly, these did not correspond to either propyluracil or propyldeoxyuracil, the anticipated most likely products of PTU desulfuration. The identity of these desulfurated metabolites of PTU in the TPO model system remains to be determined

1989-01-01

10

Synthesis of the new immunostimulating agent pidotimod (3-L-pyroglutamyl-L-thiazolidine-4-carboxylic acid) labelled with [sup 14]C-and [sup 35]S-isotopes  

Energy Technology Data Exchange (ETDEWEB)

3-l-pyroglutamyl-l-thiazolidine-4-carboxylic acid (PIDOTIMOD), a new immunostimulating agent, has been prepared labelled with [sup 14]C and [sup 35]S isotopes starting from L-[U-[sup 14]C]-glutamic acid 5 and L-[[sup 35]S]-cysteine hydrochloride 6, respectively. In the first synthesis, L-[U-[sup 14]C]-5 is converted into L-[U-[sup 14]C]-pyroglutamic acid 2, which was reacted with ethyl l-thiazolidine-4-carboxylate 3 to afford the ester 4, in turn hydrolyzed to ([sup 14]C)-PIDOTIMOD 1. In the second synthesis, L-[[sup 35]S]-6 reacted with formaldehyde to give L-[[sup 35]S]-thiazolidine-4-carboxylic acid 7, which was coupled with the activated ester of pyroglutamic acid, compound 8, to afford [[sup 35]S]-PIDOTIMOD 1. The total activity of ([sup 14]C)-PIDOTIMOD was 1.2 MCI (specific activity 5.52 MCI/mmol) and that of ([sup 35]S)-PIDOTIMOD was 1.0 MCI (specific activity 9.43 MCI/mmol). (Author).

Ferraboschi, Patrizia; Grisenti, Paride; Santaniello, Enzo (Milan Univ. (Italy). Dip. di Chimica e Biochimica Medica); Giachetti, Claudio; Zanolo, Giovanni (Biomedical Research Inst. ' ' Antoine Marxer' ' , Turin (Italy)); Signorelli, Giovanni; Coppi, Germano (Poli Industrial Center, Milan (Italy). Research Center)

1992-12-01

11

Synthesis of the new immunostimulating agent pidotimod (3-L-pyroglutamyl-L-thiazolidine-4-carboxylic acid) labelled with 14C-and 35S-isotopes  

International Nuclear Information System (INIS)

[en] 3-l-pyroglutamyl-l-thiazolidine-4-carboxylic acid (PIDOTIMOD), a new immunostimulating agent, has been prepared labelled with 14C and 35S isotopes starting from L-[U-14C]-glutamic acid 5 and L-[35S]-cysteine hydrochloride 6, respectively. In the first synthesis, L-[U-14C]-5 is converted into L-[U-14C]-pyroglutamic acid 2, which was reacted with ethyl l-thiazolidine-4-carboxylate 3 to afford the ester 4, in turn hydrolyzed to (14C)-PIDOTIMOD 1. In the second synthesis, L-[35S]-6 reacted with formaldehyde to give L-[35S]-thiazolidine-4-carboxylic acid 7, which was coupled with the activated ester of pyroglutamic acid, compound 8, to afford [35S]-PIDOTIMOD 1. The total activity of (14C)-PIDOTIMOD was 1.2 MCI (specific activity 5.52 MCI/mmol) and that of (35S)-PIDOTIMOD was 1.0 MCI (specific activity 9.43 MCI/mmol). (Author)

1992-01-01

12

Comparison of the production rate of bacteria in the rumen of buffalo calves estimated by using 32P and 35S-labelled mixed rumen bacteria as a marker  

International Nuclear Information System (INIS)

[en] 32P labelled bacteria were used to estimate their growth rate and a comparison has been made of the values obtained by using 35S labelled bacterial markers. Since 32P is a hard ?-emitter the radioactivity measurement can be made without scintillation fluid by Cherenkov counting which may be economical. (author)

1981-01-01

13

Metabolism of [14C]- and [32P]pyridoxal 5'-phosphate and [3H]pyridoxal administered intravenously to pigs and goats.  

Science.gov (United States)

To gain more information about the kinetics of vitamin B-6 metabolism in vivo, the metabolism of tracer was examined after the simultaneous intravenous administration of [32P] and [14C]pyridoxal phosphate and [3H]pyridoxal in two 93-kg pigs and two 60-kg goats. In the pigs, [14C] removal was monophasic with T1/2 of 16 and 18 min and clearance of 165 and 248 mL/min. In the goats, [14C] removal was biphasic with T1/2 of 49 and 114 min for 0-30 min and 209 and 227 min for 0.5-6 h (clearance 20 and 17 mL/min). Uptake of pyridoxal phosphate by liver and resecretion into the plasma were too small to cause a detectable decrease in the [32P]:[14C] ratio. Pyridoxal removal from plasma was similar in both species, with a half-life of approximately 12 min from 0-30 min and approximately 50 min for 0.5-3 h. Clearance of [3H]pyridoxal in the four animals ranged from 412 to 2258 mL/min. Little [14C] entered the erythrocytes. The [3H] entered readily but was converted to pyridoxal phosphate faster in the pigs than in the goats. [14C] and [3H] were excreted as pyridoxic acid at the same rate. However, during the 54 h after injection the goats excreted approximately 60% of the [14C] doses in the urine compared with approximately 30% in the pigs. About 5-10% of the [14C] and [3H] doses were recovered in goat milk over 54 h. Pyridoxal kinase activity was higher in lactating mammary tissue than in liver, kidney or muscle of goats. PMID:1732480

Coburn, S P; Mahuren, J D; Kennedy, M S; Schaltenbrand, W E; Townsend, D W

1992-02-01

14

Gene mapping on human metaphase chromosomes by in situ hybridization with /sup 3/H, /sup 35/S, and /sup 32/P labeled probes and transmission electron microscopy  

Energy Technology Data Exchange (ETDEWEB)

A method based on in situ hybridization, autoradiography and transmission electron microscopy for mapping genes on human metaphase chromosomes is presented. Successful mapping of the tandemly repeated rDNA genes and of two nucleic acid probes, N-myc and probe 3 (Kanda et al. 1983), that are amplified in a homogeneously staining region (HSR) of the neuroblastoma cell line, IMR-32 is described. By using sufficiently thin AgBr emulsions, it is possible to obtain observable grains and good resolution with probes radiolabeled with /sup 3/H, /sup 35/S, or /sup 32/P, but the former gives the best results. We observe that neither of the two probes, N-myc and probe 3, has a uniform spatial distribution along the HSR and that the distributions of the two probes differ from each other. These observations support previous studies which indicated that the formation of an HSR is a more complex process than uniform amplification of a single DNA segment to form an n-fold set of perfect tandem repeats. The present study shows that the electron microscopic method is useful for extending the results of light microscopic studies for problems where higher resolution mapping is needed.

Li Changben; Wu, M.; Mergitich, I.S.; Davidson, N.

1986-01-01

15

Gene mapping on human metaphase chromosomes by in situ hybridization with 3H, 35S, and 32P labeled probes and transmission electron microscopy  

International Nuclear Information System (INIS)

[en] A method based on in situ hybridization, autoradiography and transmission electron microscopy for mapping genes on human metaphase chromosomes is presented. Successful mapping of the tandemly repeated rDNA genes and of two nucleic acid probes, N-myc and probe 3 (Kanda et al. 1983), that are amplified in a homogeneously staining region (HSR) of the neuroblastoma cell line, IMR-32 is described. By using sufficiently thin AgBr emulsions, it is possible to obtain observable grains and good resolution with probes radiolabeled with 3H, 35S, or 32P, but the former gives the best results. We observe that neither of the two probes, N-myc and probe 3, has a uniform spatial distribution along the HSR and that the distributions of the two probes differ from each other. These observations support previous studies which indicated that the formation of an HSR is a more complex process than uniform amplification of a single DNA segment to form an n-fold set of perfect tandem repeats. The present study shows that the electron microscopic method is useful for extending the results of light microscopic studies for problems where higher resolution mapping is needed. (orig.)

1986-01-01

16

Metabolism of /sup 35/S- and /sup 14/C-labeled propylthiouracil in a model in vitro system containing thyroid peroxidase  

Energy Technology Data Exchange (ETDEWEB)

In previous communications we described an in vitro model system containing highly purified thyroid peroxidase (TPO) for studying the mechanism of inhibition of thyroid hormone biosynthesis by the antithyroid drugs, 6-propylthiouracil (PTU) and 1-methyl-2-mercaptoimidazole (MMI). We showed that inhibition of iodination of thyroglobulin in this system may be reversible or irreversible depending on the relative concentrations of iodide and drug and the TPO concentration. Metabolism of the drugs occurred under both conditions, but was more limited under irreversible conditions of inhibition. It was of interest to examine the nature of the drug metabolites associated with reversible and irreversible conditions of inhibition. For this purpose we have employed the 35S- and 14C-labeled drugs and a recently developed reverse phase HPLC procedure. Results of a similar study with MMI were reported in an earlier communication. In the present study we report our findings with PTU. Under conditions of reversible inhibition, PTU was readily metabolized and by 15 min was reduced to a few percent of the starting value. The earliest detectable metabolite with both (35S)- and (14C)PTU was the disulfide, which reached a peak in about 15 min and then slowly declined. Coincident with the decline in the disulfide was the appearance of more polar metabolites. In the case of (35S)PTU, these corresponded to sulfate/sulfite, PTU sulfonate, and a product tentatively identified as PTU sulfinate. The latter two were also observed as 14C-labeled metabolites produced from (14C)PTU. Two nonpolar desulfurated 14C-labeled metabolites were also observed. Surprisingly, these did not correspond to either propyluracil or propyldeoxyuracil, the anticipated most likely products of PTU desulfuration. The identity of these desulfurated metabolites of PTU in the TPO model system remains to be determined.

Taurog, A.; Dorris, M.L.; Guziec, F.S. Jr.; Uetrecht, J.P.

1989-06-01

17

Influence of boron nutrition on net uptake and efflux of 32P and 14C-glucose in Helianthus annuus roots and cell cultures of Daucus carota  

International Nuclear Information System (INIS)

32P and 14C-glucose uptake were reduced under B deficiency in both Daucus cell suspensions and Helianthus roots. Similarly, efflux rates were found to be smaller in B deficient material. Efflux rates tended to be more affected than net uptake of both 32P and glucose. This may explain why sunflower roots showed a higher glucose net uptake immediately after transferring from a B sufficient to a B deficient nutrient solution. The data confirm earlier findings of B as an essential element for membrane function and integrity. B-deficiency effects could be reversed by the addition of B within less than one hour. Cell suspensions reacted similarly to roots with respect to B deficiency and may thus be suitable for further research on B deficiency effects. (orig.).

1985-01-01

18

In vitro incorporation of 1-14C-acetate and 32P-orthophosphoric acid into phospholipids at various stages of growth of Pythium irregulare  

International Nuclear Information System (INIS)

Pythium irregulare showed an increase (11.6 to 38.9%) in total lipids and a simultaneous decrease (40.5 to 7.3%) in phospholipid content of total lipids during growth period of 15 days. Incubation of fungus of different ages viz. 2,3,4,5,7,9,11,13 and 15 days old with 32P-orthophosphoric acid for 4 hr showed that the specific activity of mycelium was maximum with the 5-days old fungus. Highest content of total and polar lipids was, however observed with the 3 days old sample. Incubation studies with sodium acetate 1-14C with fungus of various stages of growth showed maxima of specific activity in mycelium, total, polar and non-polar lipids on the 5th day. From specific activity estimation of 32P and 14C in each of the phosphatides, it was concluded that the labels were incorporated at a higher rate into phosphatidyl enthanolamine during early stages of growth. The reverse was true for the later period of growth, indicating that phosphatidyl ethanolamine was possibly converted to phosphatidyl choline. (auth.).

1979-01-01

19

14C-leucine, 35S-methionine 3H-lysine, 14C-3-O-methyl-glucose and 3H-ABA transport in developing maize embryos  

International Nuclear Information System (INIS)

To understand the development of the maize embryo the authors have studied the transport of 14C-leucine, 35S-methionine, 3H-lysine, 14C-3-O-methyl-glucose, and 3H-abscisic acid in isolated Tx5855 or sweet corn embryos. pH optima ranged from pH 4.2 for methionine, pH 5.0 and pH 5.75 for leucine, pH 6.5 for lysine, to pH 5.5-6.5 for 3-O-methyl-glucose. Uptake rates of all radiolabelled compounds were linear during the first three hrs of incubation. Only leucine showed a saturable component with an apparent Km 10-4M. When comparing the uptake rates during the entire range of embryogenesis in inbred line TX5855, leucine and 3-O-methyl-glucose show parallel changes in uptake rates (moles/gm fw/hr or moles/embryo/hr). In isolated sweet corn embryos, ABA (10-5 to 10-8M) showed no effect on 3-O-methyl-glucose or methionine uptake, but showed a 25-40% increase in leucine and lysine uptake as compared with no ABA addition. These characterizations of metabolite uptake provide a basis for the design of future studies with isolated maize embryos

1987-01-01

20

Influence of boron nutrition on net uptake and efflux of /sup 32/P and /sup 14/C-glucose in Helianthus annuus roots and cell cultures of Daucus carota  

Energy Technology Data Exchange (ETDEWEB)

/sup 32/P and /sup 14/C-glucose uptake were reduced under boron (B) deficiency in both Daucus cell suspensions and Helianthus roots. Similarly, efflux rates were found to be smaller in B deficient material. Efflux rates tended to be more affected than net uptake of both /sup 32/P and glucose. This may explain why sunflower roots showed a higher glucose net uptake immediately after transferring from a B sufficient to a B deficient nutrient solution. The data confirm earlier findings of B as an essential element for membrane function and integrity. B-deficiency effects could be reversed by the addition of B within less than one hour. Cell suspensions reacted similarly to roots with respect to B deficiency and may thus be suitable for further research on B deficiency effects.

Goldbach, H.

1985-01-01

 
 
 
 
21

Use of 35S nucleotides in Sanger dideoxy sequencing  

International Nuclear Information System (INIS)

Sequencing DNA using the Sanger Dideoxy Chain Termination Method and 35S-labeled deoxyribonucleotide analogs represents the current 'state-of-the-art' in gene sequencing technology. The application of 35S-nucleotides has many significant advantages over older radiolabeling methods that use 32P-nucleotides. The lower emission energy and longer half life of 35S (87 days vs. 14 days for 32P) provide greater convenience and flexibility since no special shielding is necessary, and greater economy since waste due to radioactive decay is kept to a minimum. The lower ? emission energy of 35S produces sequencing autoradiograms with sharper bands and greater clarity, so that more useable sequence information can be gathered from each experiment. Labeling with 35S improves the stability of DNA, which results in lower autoradiographic background, especially in labeled DNA samples that are not used immediately. 9 refs.; 5 figs

1986-01-01

22

A study of 32P-phosphate uptake in a plant by a real-time RI imaging system  

International Nuclear Information System (INIS)

[en] It is very important to visualize the process of nutrient absorption and distribution to study the physiological activity of the plant. We developed a real-time radioisotope (RI) imaging system, where RI tracers were applied to the plant sample. This system allowed the quantitative measurement concerning the uptake of nutrients labeled with radioisotopes, such as 45Ca, 35S, 32P and 14C as long as several days. The ?-rays emitted from the sample were converted to light by a CsI(Tl) scintillator and were guided to a highly sensitive CCD camera. The scintillator surface was covered with an Al plate to avoid LED light penetration but allow selected ?-ray penetration. We employed Lotus japonicus for the plant sample and observed the 32P-phosphate absorption in roots and the accumulation to the aboveground part of the plant. The environment condition of daytime and night was simulated by the ON/OFF of LED timer and the accumulation manner of the 32P-phosphate in roots and leaves during daytime and night was analyzed. The accumulation of 32P-phosphate in leaves was highly dependant on light irradiation and higher when the LEDs was turned on, whereas the absorption of 32P-phosphate in root was higher when the LEDs was turned off. The transfer function concerning the transportation of phosphate within the plant during the developmental stage was obtained from the analysis of 32P uptake images. We are now trying to get specific moving images of each radioisotope when two kinds of isotopes, such as 32P and 32S, were applied at the same time to the plant, through an image analysis. (orig.)

2009-12-04

23

Natural 14C variations  

International Nuclear Information System (INIS)

This thesis deals with the natural variations in the atmospheric 14C activity, their geophysical origin and their impact on radiocarbon dating. Studies confirm the idea that one is dealing with a mechanism of a certain regularity. The correlation between a 14C variation during the Little Ice Age and the absence of sunspots on the solar surface suggest the sun to be responsible for some kind of modulation of the galactic cosmic ray spectrum. The background of a changing natural 14C level is relevant when studying the antropogenic perturbation of the atmospheric 14C concentration by the addition of CO2 from fossil fuel combustion. The results presented point to a Suess effect over the past 150 years of about 20 per thousand, but also show a local dilution effect. If this local effect is present over large continental parts of the Northern Hemisphere this will put limits to the use of tree ring 14C measurements for testing carbon reservoir models. Finally the influence of 14C variations upon the interpretations of 14C dates for archaeological and geological purposes has been investigated. It is shown that care must be taken especially in the interpretation of highly accurate 14C data of material only covering a few years of growth. One geological example illustrates that 14C variations can easily be interpretated as alternating fast and slow rises of the sea level. (Auth.)

1981-01-01

24

Synthesis of captopril labeled with 2H, 3H, 14C, or 35S  

International Nuclear Information System (INIS)

Three routes for the synthesis of labelled captopril are described. The preferred route can be selected with regard to the desired position of the label. The chemical and radiochemical stability of captopril is discussed. An isotope effect was found in the IR spectrum when the side chain carbonyl carbon was labelled with carbon-14. (author)

1988-01-01

25

The use of radioisotopes 32P and 35S in studies on behavior of pesticides in the soil  

International Nuclear Information System (INIS)

Radioisotopes were used to determine the possibility and extent of contamination of underground waters by pesticides used in agriculture. The sorption and dynamics of the migration of two organo-phosphorous pesticides, fenitrothion and malathion, were studied. These two products are currently being proposed as agricultural pesticides. They would replace other substances of the chrlorinated hydrocarbon group, including DDT, which are more dangerous to health, but are themselves source of potential contamination of underground waters. Studies were carried out on different types of soil: sandy and clay. Isotherms were determined for the sorption of the pesticides examined, together with their distribution coefficients, Kd. The effects of pH values, exchangeable ions, organic substances (organic C and N) and the mechanical composition of the sorption of fenitrothion and malathion were studied.

1974-06-24

26

Synthesis of ?-sitosterol-14C  

International Nuclear Information System (INIS)

[en] The method of synthesis of ?-sitosterol-4-14C starting with the enolactone 4-oxa-5-sitosten-3-one is discussed. Methyl-14C magnesium iodide is utilized for the introduction of the label. The authors selected this method for the isolation of ?-sitosterol-14C, introducing a series of changes into the original method. The authors discuss obtaining sitostenone, the ketoacid of sitostenone, the enol-lactone of the ketoacid of sitostenone, sitostenone-4-14C (by different methods), the enol-acetate of sitostenone-14C, and ? sitosterol-4-14C

1986-01-01

27

Kinetics of 35S uptake in sugarcane  

International Nuclear Information System (INIS)

Kinetics of 35S uptake was studied in excised shoot roots in three sugarcane cultivars Co 419, Co 997 and Co 1148 in relation to concentration. There were at least four distinct phases in uptake in the concentration range from 0.01 mM to 5.00 mM. At higher concentration ranges, cultivars did not differ in kinetics of 35S uptake. However, at lower concentrations Co 1148 was more efficient in 35S uptake compared to Co 419 and Co 997. (author).

1983-01-01

28

Preparation of /sup 35/S labelled thiosemicarbazone  

Energy Technology Data Exchange (ETDEWEB)

A /sup 35/S labelled thiosemicarbazone is prepared, on a millimole scale by reacting labelled thiocyanate with hydrazine sulfate in ethanolic medium. The hydrazine thiocyanate so formed is then condensed with aldehyde to form the thiosemicarbazone.

Renault, H. (I.N.S.E.R.M. Hopital Saint-Antoine, 75 - Paris (France)); Cals, M.J. (Hopital Vaugirard, 75 - Paris (France))

1982-11-01

29

Isolation of (-)-menthol-14C and (+)-neomenthol-14C  

International Nuclear Information System (INIS)

Uniformly labelled (-)-menthol-14C and (+)-neomenthol-14C were isolated and purified from the oil of peppermint plants grown in a 14CO2 chamber by preparative high performance liquid chromatography. This procedure is much simpler than previous methods and allows for large amounts of labelled oil to be processed. (author)

1982-01-01

30

Spectroscopy of 32P, ch. 2  

International Nuclear Information System (INIS)

[en] Spins, parities and transition probabilities of levels in 32P at Esub(x) 3.5 MeV have been determined with the 29Si(?,p?)32P reaction at bombarding energies of Esub(?)=12.80, 12.93 and 16.30 MeV. Proton-gamma angular correlation experiments and DSA lifetime measurements lead to six unambiguous spin assignments and to many spin limitations. The measured mixing and branching ratios yield many transition strengths for dipole and electric quadrupole transitions. Five, sofar unknown, energy levels are reported. A doublet at 4.03 MeV excitation energy and a high-spin state (Esub(x)=4.27 MeV; Jsup(?)=5-) were observed. Shell-model predictions have been compared to the present experimental results. (Auth.)

1976-03-24

31

Evidence against a 17 keV neutrino from 35S beta decay  

International Nuclear Information System (INIS)

We have searched for the effect of a neutrino of mass 17 keV/c2 in the beta decay of 35S with an appartus incorporating a high-resolution solid-state detector and a superconducting solenoid. The experimental mixing probability, sin2?=-0.0004±0.0008(stat) ±0.0008(syst), is consistent with zero, in disagreement with several previous experiments. Our sensitivity to neutrino mass is verified by measurements with a mixed source of 35S and 14C, which artificially produces a distortion in the beta spectrum similar to that expected from the massive neutrino.

1993-01-01

32

The method and apparatus for S and 35S analysis in steel labelled by 35S  

International Nuclear Information System (INIS)

The method of volumetric and radiometric analysis of S and 35S present in steel labelled by 35S was elaborated. SO2 and C02 obtained from burned out steel samples can be separated from other gases at temperature -1960C and SO2 from CO2 at temperature -780C. This method allows to measure the 35SO2 activity in a proportional counter filled up by a propane - butane (300 Tr) counting gas. A proportional counter is in a coincidence-anticoincidence system. The measurement efficiency obtained is /69+-3.5/% when 35So2 pressure in counter does not exceed 5 Tr. Time needed for one analysis of S and 35S in the sample is about 0.5 H. (author)

1976-01-01

33

Synthesis of ammonium [14C]thiocyanate  

International Nuclear Information System (INIS)

Ammonium [14C]thiocyanate was prepared from potassium [14C]cyanide with a radiochemical yield of 90%. [14C]hydrocyanic acid, generated from potassium [14C]cyanide by sulphuric acid, reacts with aqueous ammonia and elemental sulphur in the presence of trace amounts of ammonium sulphide to yield ammonium [14C]thiocyanate. (author)

2003-01-01

34

Spring mobilization of storage 32P in gingko trees  

International Nuclear Information System (INIS)

[en] The labelled phosphorus was supplied to the roots of 1-year-old gingko (Gingko biloba L.) trees during dormant period in the glass house and the mobilization of stored 32P determined in the following early growing season. 32P was detected in all growing tissues, the level of radioactivity being greatest in the root tissues over the 4-week sampling period. At dormant period, only 0.44% of 32P was found in storage tissues (bark, wood and bud) although the accumulation of 32P occurred especially in root tissues. During early growing season, 32P was transported from root tissues to new growing tissues (buds). Eight percent of the initial 32P in storage tissues was transported to new growing tissues. During this period, the 32P in the bud tissues increased about 12 fold. (author)

1978-01-01

35

Behaviors of 14C-butachlor, 14C-chlorpyrifos and 14C-DDT in Rana japonica japonica Guenther  

International Nuclear Information System (INIS)

The research on the behaviors of 14C-butachlor, 14C-chlorpyrifos and 14C-DDT in the frog Rana japonica japonica Guenther was carried out. After administrated per os to the frogs in doses of 380, 347, 363 Bq/g, 14C-butachlor, 14C-chlorpyrifos and 14C-DDT, were distributed respectively to various organs within 24 h with specific accumulating organs as gallbladder, intestine and intestine, relevantly to the pesticides described. Compared to that in gallbladder and intestine, the radioactivity of many organs was extremely low, and this might due to the characters of the pesticides. Analysis of the metabolites of 14C-DDT in frog at 24 th hr demonstrated that DDT was difficult to be degraded. Most 14C-butachlor, 14C-chlorpyrifos 14C-DDT in liver and fat or ovary of frog was extractable with acetone. However, there were some differences between the pesticides, and the organs as well. And 14C-butachlor, 14C-chlorpyrifos or 14C-DDT were better bound in liver than in fat

2002-01-01

36

Development of the new distillation method for 32P production  

International Nuclear Information System (INIS)

[en] 32P as a pure ?-ray emitter is widely used in nuclear medicine, genetic engineering, biological research, etc. The production process of 32P is mainly based on sulfur distillation after natural 32S(n,p)32P reaction. In this study, a new distillation process for 32P production has been developed and applied for production of 32P. Distillation and condensation of sulfur in the capsule occurred at about 180 .deg. C under 0.1 torr pressure and the distillation rate of sulfur dependened on the temperature of distillation zone. In the typical case, it took 1.5 - 2 hours for the complete distillation of 1 g of sulfur under 0.1 torr pressure and the recovery yield of sulfur was near 100%. Sulfur target in the evacuated capsule was irradiated for 72 hours in HANARO reactor and the fast neutron flux of irradiation hole was 2.8x1014 n/cm2·sec. The irradiated target was distilled at 220 .deg. C and then leached out 32P residue on the capsule surface. The yield of 32P was 8 mCi per 1g of natural sulfur and the qulity analysis of the final product was carried out for radionuclidic purity, radiochemical purity and solid residue. It is expected that the developed process can be useful for production of around 100 mCi 32P and also can be applied to produce 33P using enriched 33S targets

2001-01-01

37

/sup 35/S-glycosaminoglycan and /sup 35/S-glycopeptide metabolism by diabetic glomeruli and aorta  

Energy Technology Data Exchange (ETDEWEB)

/sup 35/S-glycosaminoglycan metabolism by glomeruli isolated from streptozotocin-diabetic and control rats was studied in vivo and in vitro. Total /sup 35/S-glycosaminoglycan synthesis and retention in the matrix by diabetic glomeruli was reduced while degradation was increased. /sup 35/S-glycosaminoglycan content of isolated GBM was similarly decreased. Whereas /sup 35/S-glycosaminoglycan content of glomeruli and GBM was decreased after in vitro incubation with /sup 35/SO/sub 4/, a larger proportion of total /sup 35/S-glycosaminoglycans was found in the incubation medium from diabetic glomeruli. Both control and diabetic glomeruli synthesize /sup 35/S-labeled glycopeptides, the quantity from diabetic glomeruli being reduced. Aorta from /sup 35/SO/sub 4/-injected diabetic rats also synthesized reduced quantities of /sup 35/S-glycosaminoglycans. There were no preferential metabolic alterations of species of /sup 35/S-glycosaminoglycans by diabetic glomeruli or aortas. These studies suggest that synthesis of /sup 35/S-glycosaminoglycans and /sup 35/S-glycopeptides by diabetic glomeruli are altered by disturbances of both synthetic as well as degradative pathways. An alteration of /sup 35/S-glycosaminoglycans interaction with matrix components in diabetes is postulated.

Brown, D.M.; Klein, D.J.; Michael, A.F.; Oegema, T.R.

1982-05-01

38

Oxidative chlorination of [U14C] phenol  

International Nuclear Information System (INIS)

The preparation of [U-14C] 2,4-dichlorophenol was formerly achieved by a Sandmeyer reaction with [U-14C] 2,4-dichloroaniline but this sensitive step gave unreproductive yields in our hands. We describe another route to [U-14C] 2,4-dichlorophenol by the oxidative chlorination of [U-14C] phenol. This shorter route is safer and gives satisfactory yields. The nature of the oxidizer is discussed. (author).

1996-01-01

39

Preparation of 35S-labelled albendazole sulfinyl (ABZO)  

International Nuclear Information System (INIS)

[en] Albendazole is an important insecticide and albendazole sulfinyl (ABZO) is the effective component. In order to investigate its absorption, distribution and excretion in vivo, 35S-labelled tracer was necessary for experiment. 35S-labelled intermediate was made by isotopic exchange. The radiochemical yield was over 90%. After purification by TLC, the radiochemical purity of the 35S-ABZO determined by PC and electrophoresis was over 95%

1992-01-01

40

Bioconversion of ?-[14C]Zearalenol and ?-[14C]Zearalenol into [14C]Zearalenone by Fusarium roseum Gibbosum  

International Nuclear Information System (INIS)

Cultures of Fusarium roseum Gibbosum on rice were treated with [14C]zearalenone, ?-[14C]zearalenol, or ?-[14C]zearalenol to determine whether a precursor-product relationship exists among these closely related fungal metabolites. Culture extracts were purified by silica gel column chromatography and fractionated by high-pressure liquid chromatography, and the level of radioactivity was determined. Within 7 days, the ?-[14C]zearalenol was converted to zearalenone, and no residual ?-[14C]zearalenol was detectable. Most of the ?-[14C]zearalenol added was also converted into zearalenone within 14 days. In cultures treated with [14C]zearalenone, no radioactivity was noted in any other components.

1984-01-01

 
 
 
 
41

Synthesis of [pyrrolidine-14C]-cotinine from 14C-nicotine  

International Nuclear Information System (INIS)

The synthesis and purification of 14C-cotinine is reported. 14C-nicotine is converted into 14C-dibromocotinine. Debromination of the latter results in 14C-cotinine which is purified by high performance liquid chromatography. The total radioactive yield of the synthesis is 44.8%. Starting from 500 ?Ci 14C-nicotine, 224 ?Ci 14C-cotinine is obtained with a specific activity of 0,23 mCi/mmol. (Author)

1989-01-01

42

Preparation of [14C]uridine 5'-diphosphate and [14C]guanosine 5'-diphosphate  

International Nuclear Information System (INIS)

Procedures have been developed for the routine enzymatic synthesis of [14C]UDP and [14C]GDP from commercially available enzymes and [14C]UMP and [14C]GMP. Using high pressure liquid chromatography, the products are recovered in high yield (60-80%) and with high purity. The [14C]UDP and [14C]GDP are utilized as substrates for ribonucleotide reductase.

1984-01-01

43

Synthesis of L-[35S] homocysteine thiolactone hydrochloride  

International Nuclear Information System (INIS)

L-[35S]Homocysteine thiolactone has been synthesized by demethylation of L-[35S]Methionine with sodium in liquid ammonia and subsequent lactonisation in acid solution. The radiochemical yield of the carrier added synthesis was in the range of 45 to 50% with a radiochemical purity higher than 96%. (author)

1989-01-01

44

Synthesis of L-(/sup 35/S) homocysteine thiolactone hydrochloride  

Energy Technology Data Exchange (ETDEWEB)

L-(/sup 35/S)Homocysteine thiolactone has been synthesized by demethylation of L-(/sup 35/S)Methionine with sodium in liquid ammonia and subsequent lactonisation in acid solution. The radiochemical yield of the carrier added synthesis was in the range of 45 to 50% with a radiochemical purity higher than 96%. (author).

Hamacher, K.

1989-03-01

45

A convenient synthesis of 14C-cotinine from 14C-nicotine  

International Nuclear Information System (INIS)

A convenient synthesis with analytical monitoring of 14C-cotinine is reported. 14C-Nicotine was converted into 14C-dibromocotinine hydrobromide perbromide. Debromination, achieved by using Zn dust/acetic acid, resulted in high yields (71%) of 14C-cotinine. (author)

1991-01-01

46

A convenient synthesis of sup 14 C-cotinine from sup 14 C-nicotine  

Energy Technology Data Exchange (ETDEWEB)

A convenient synthesis with analytical monitoring of {sup 14}C-cotinine is reported. {sup 14}C-Nicotine was converted into {sup 14}C-dibromocotinine hydrobromide perbromide. Debromination, achieved by using Zn dust/acetic acid, resulted in high yields (71%) of {sup 14}C-cotinine. (author).

Desai, D.H.; Djordjevic, M.V.; Amin, S. (American Health Foundation, Valhalla, NY (USA). Naylor Dana Inst. for Disease Prevention)

1991-03-01

47

The formation of 35S labeled dithiocarbamate by the reaction of thiurumdisulfide with inorganic sulfide-35S  

International Nuclear Information System (INIS)

[en] The decomposition reaction of thiuramdisulfide with 35S labelled inorganic sulfide ion and the distribution of 35S in the products were investigated. No radioactivity was found in the dithiocarbamate in the reaction at 300C. This result shows that the decomposition of thiuramdisulfide is caused by the nucleophilic attack of sulfide ion on a -S-S- bond as is generally accepted. In the reaction at 800C 35S labelled dithiocarbamate was yielded, and its radioactivity was increased by additional heating of the reaction mixture. The incorporation of 35S into the product was explained in terms of the isotope exchange reaction with 35SH-. Corresponding 35S labelled dithiocarbamates were isolated from the reaction mixtures. (author)

1975-01-01

48

Present status of the 32P test in ophthalmology  

International Nuclear Information System (INIS)

Studies agreed with Hagler and Jarrett that the 32P test continues to be the most accurate test devised for the diagnosis of malignant melanoma. The one false negative test was one in which the probe could not be accurately placed because the lesion was pedunculated and adjacent to the optic disc. There were no false positive tests in this series. An attempt to correlate the percentage uptake with the cell type of the melanoma was unsuccessful. Perhaps further studies may elucidate what should be a possible connection between the metabolic activity of a given tumor and amount of radiophosphorus uptake within the lesion. The relative quantities and localizations of 32P in normal choroid, retina, cornea, lens, tumor, and vitreous are being studied. A significant amount of 32P may enter the vitreous cavity around the tumor. This knowledge should lead to a more precise understanding of the true basis for the clinical test as it is now performed. (U.S.)

1973-05-03

49

Synthesis of [14C]Zolpidem  

International Nuclear Information System (INIS)

[en] The synthesis of [14C]Zolpidem, a new hypnotic agent having a non-benzodiazepine structure, is described. This compound was synthesised in a 64% overall radiochemical yield from potassium [14C]cyanide and with a specific radioactivity of 56 mCi/mmol. It was used for pharmacokinetic and drug metabolism studies. (author)

1986-01-01

50

Synthesis of 14C-labelled isophosphamide  

International Nuclear Information System (INIS)

Isophosphamide labelled with 14C in the chloroethyl group attached to the exocyclic nitrogen has been synthesised by treatment of N-3-hydroxypropylaziridine with phosphorus oxychloride and reaction of the resulting 2-chloro-3-(2-chloroethyl)tetrahydro-2H-1,3,2-oxazaphosphorine-2-oxide with [1-14C]-2-chloroethylamine. (author)

1981-01-01

51

Formation of /sup 14/C-asparagine from /sup 14/C-precursor in mulberry leaves  

Energy Technology Data Exchange (ETDEWEB)

Since a remarkable accumulation of asparagine in the young leaves of mulberry has been observed, the formation of /sup 14/C-asparagine from /sup 14/C-labeled substrates in young leaves was examined in comparison with that in the mature leaves. /sup 14/C-aspartic acid and /sup 14/C-succinic acid expected as active precursors for asparagine biosynthesis, and /sup 14/C-sucrose as respiratory substrates were fed respectively to the disks of young or mature leaves of mulberry. Although /sup 14/C-succinic acid was actively converted to /sup 14/C-asparagine, no significant amount of /sup 14/C-asparagine was formed from /sup 14/C-aspartic acid in two hours of feeding period. The rate of formation of /sup 14/C-asparagine from /sup 14/C-succinic acid in the mature leaves was slightly higher than that in the young leaves. Amino acids other than asparagine acquired /sup 14/C from /sup 14/C-labeled substrates were mainly aspartic acid, glutamic acid, alanine and ..gamma..-amino butyric acid in both of the leaves. Intending to accelerate the formation of asparagine in the leaves, ammonium ion was supplied to culturing solution as only source of nitrogen and plants were grown for two weeks in that solution before /sup 14/C-labeled substrates feeding experiments. Supplying of ammonium ion brought about enhanced accumulation of asparagine in the young leaves, and caused remarkable formation of /sup 14/C-asparagine from /sup 14/C-aspartic acid in both of the leaves. However, the rate of /sup 14/C-asparagine formation from /sup 14/C-aspartic acid in the young leaves did not exceed that in the mature leaves.

Yamashita, T. (Sericultural Experiment Station, Yatabe, Ibaraki (Japan))

1981-08-01

52

Measurement of fecal /sup 14/C excretion  

Energy Technology Data Exchange (ETDEWEB)

Simultaneous measurements of fecal /sup 14/C and expired /sup 14/CO/sub 2/ in the breath are necessary to evaluate patients with various ileal abnormalities and bile salt malabsorption. Following the oral ingestion of the labeled bile acid, glycine-(I-/sup 14/C)cholic acid, detection of increased fecal /sup 14/C without abnormal expiration of /sup 14/CO/sub 2/ identifies patients with ileal resection. This contrasts with the normal fecal /sup 14/C content and abnormal expired /sup 14/CO/sub 2/ found in patients with bacterial overgrowth. Fecal /sup 14/C content was determined by utilizing Van Slyke combustion of the specimen and trapping the liberated /sup 14/CO/sub 2/ with Scintisorb C. The method is simple, rapid, and accurate, and expands the diagnostic usefulness of the bile salt absorption test.

Kumaran, K.A.; Wiener, N.S.; Katz, J.B.

1982-11-01

53

Measurement of fecal 14C excretion  

International Nuclear Information System (INIS)

Simultaneous measurements of fecal 14C and expired 14CO2 in the breath are necessary to evaluate patients with various ileal abnormalities and bile salt malabsorption. Following the oral ingestion of the labeled bile acid, glycine-[I-14C]cholic acid, detection of increased fecal 14C without abnormal expiration of 14CO2 identifies patients with ileal resection. This contrasts with the normal fecal 14C content and abnormal expired 14CO2 found in patients with bacterial overgrowth. Fecal 14C content was determined by utilizing Van Slyke combustion of the specimen and trapping the liberated 14CO2 with Scintisorb C. The method is simple, rapid, and accurate, and expands the diagnostic usefulness of the bile salt absorption test

1982-01-01

54

Study of the reaction {sup 14} C (p,p) {sup 14} C; Estudio de la reaccion {sup 14} C (p,p) {sup 14} C  

Energy Technology Data Exchange (ETDEWEB)

The study of the elastic scattering of polarized protons in {sup 14} C, it has been very limited. Some angular distributions exists to low energy, as well as measures of excitation functions to several angles for the differential section and the vectorial analyzer power. A detailed study of the elastic scattering of protons by {sup 14} C, it give us experimental information of the excited states in {sup 15} N. The study of these states, is since of considerable interest it is not very easy to obtain a target of {sup 14} C also in a reaction {sup 14} C (p,p) {sup 14} C is possible to obtain information of levels in {sup 15} N to an excitation energy E{sub X} >14.95 MeV. (Author)

Murillo, G.; Ramirez, J.; Avila, O.; Fernandez, M. [ININ, A.P. 18-1027, Mexico D.F. (Mexico); Darden, S.E.; Prior, R.P.; Sen, S. [University of Notre Dame, Indiana (United States)

1991-04-15

55

Synthesis of 14C labelled acrylic derivatives: diethyl [3-14C] methylidenemalonate and isobutyl [3-14C] cyanoacrylate  

International Nuclear Information System (INIS)

Isobutyl [3-14C] cyanoacrylate and diethyl [3-14C] methylidenemalonate were synthesized by the intermediate of their protective Diels-Alder adduct with anthracene. These adducts were obtained in a one-pot procedure by Knoevenagel condensation of [14C] paraformaldehyde with isobutyl cyanoacetate and diethyl malonate respectively in the presence of a basic catalyst and anthracene. The adducts are stable crystalline compounds easily purified by recrystallization. The olefinic target compounds were obtained in high chemical and radiochemical purity (>99%) by thermolysis at 2200C in mineral oil in the presence of maleic anhydride. (author).

1989-01-01

56

Synthesis of [3-14C]- and [phenyl-U-14C] olaquindox  

International Nuclear Information System (INIS)

Olaquindox is a new feed additive. [14C]Olaquindox, labelled in different positions, was needed for tracer-studies of pharmacokinetics, biotransformation and residues in several species of animals. 2-[N-(2-hydroxethyl)-carbamoyl]-3-methyl-[3-14C]quinoxaline-1,4-dioxide([3-14C]Olaquindox) was synthesized from barium[14C]carbonate (22 mmoles; 1.15 Ci) via [1-14C]acetic acid, sodium[1-14C]acetate, [1-14C]acetylchloride, ethyl[3-14C]acetoacetate and 2-carbethoxy-3-methyl-[3-14C]quinoxaline-1,4-dioxide with an overall yield of 10%, based on barium[14C]carbonate. The radiochemical purity was better than 98% (tlc). The specific activities of three preparations were 10.5, 8.4 and 5.45 ?Ci/mg respectively. [phenyl-U-14C]Olaquindox was synthesized starting from [U-14C]aniline (19.8 mmoles; 284.4 mCi). Intermediate products were N-acetyl[U-14C]aniline, 2-nitro-N-acetyl[U-14C]aniline, 2-nitro[U-14C]aniline and [U-14C]benzofurazanoxide. The total yield was 50% as calculated for [U-14C]aniline. At calibration samples of two preparations showed specific activities of 49.5 and 11.1 ?Ci/mg respectively. The radiochemical purity was checked by tlc and exceeded 98%. (author)

1981-01-01

57

14C concentrations in tree stems, 1  

International Nuclear Information System (INIS)

The 14C concentrations in trees sampled at various latitudes were measured with a Tandetron Accelerator Mass Spectrometer at Nagoya University. The growing periods of the parts for 14C measurements were estimated by the relationship between meteorological conditions and the appearance of anatomical features of annual rings such as false rings, latewood formation, and so on. The following results were obtained: 1. The latitude dependence of the 14C variation is found in tree stems as well as in the atmosphere. 2. The 14C concentrations in tree stems are almost equal to those in the atmosphere at the latitude where the tree had grown and at the time when the sampled section is formed. Therefore the 14C concentrations in the atmosphere are estimated by those of the tree stems. 3. The time when the 14C concentration in the tree showed its maximum value has difference of 1 - 2 years with that of the latitude where the tree had grown. 4. This phenomena seemed to be related closely with the mechanism of global mixing of 14CO2 produced by atmospheric nuclear weapon tests. This mechanism causes a time lag of 14C variation between northern and southern hemisphere. (author).

1993-01-01

58

? decay of excited states in 14C  

International Nuclear Information System (INIS)

Measurements of the 14C(14C,14C[10Be+?]) breakup reaction have been made at a beam energy of 98.2 MeV. The studies were performed with two charged-particle telescopes that permitted the energy, mass, charge, and emission angle of each detected particle to be determined. A series of 14C excited states at energies of 14.3, 14.8 15.6, 16.4, 17.3, 18.6, 19.8, 20.6, and 21.6 MeV was observed to decay to either the 10Be ground state or the first excited states. Angular correlation measurements suggest an assignment of J?=3- for the 15.6 MeV state.

2007-01-01

59

Synthesis of tetramethylethylenedioxydi-[14C]-methylsilane  

International Nuclear Information System (INIS)

[en] The synthesis of tetramethylethylenedioxydi-[14C]-methylsilane, a molecule used as a plasticizer in polysiloxane/silica blends, is described. This compound was synthesized in a 48% overall radiochemical yield from [14C]-methyl iodide and with a specific radioactivity of 33 ?Ci/mmole. It was used to study the competitivity of adsorption of plasticizers and models of polydimethylsiloxanes towards silicas. (author)

1990-01-01

60

Calculation of measurement uncertainty for counting of {sup 35}S  

Energy Technology Data Exchange (ETDEWEB)

{sup 35}S presented in radioisotope (RI) waste from various institutes was separated and analyzed by LSC. The measurement uncertainty was evaluated for the sample, beta ({beta}-) counting and recovery. The sample uncertainty was calculated the sample mass (7.8 x 10{sup -4}%), the concentration of the tracer (0.618%) and used volume of tracer (0.520%). The counting uncertainty was also calculated the precipitate mass (0.973%) and the counting of {sup 35}S (2.37%). The recovery uncertainty was 3.61%. Then the relative sum uncertainty was 5.13% and the expanded uncertainty was 10.26%.

Lee, Heung Nae; Kim, Sang Wook; Park, Jeong Hoon; Hur, Min Goo; Kim, In Jong; Jung, Soon Jae; Kim, Hee Jung; Lee, Eun Sun; Choi, Sang Moo; Yang, Seung Dae [Korea Atomic Energy Research Institute, Jeongeup (Korea, Republic of)

2009-12-15

 
 
 
 
61

Uptake and distribution of 14C during and following exposure to [14C]methyl isocyanate  

International Nuclear Information System (INIS)

Guinea pigs were exposed to [14C]methyl isocyanate (14CH3-NCO, 14C MIC) for periods of 1 to 6 hr at concentrations of 0.5 to 15 ppm. Arterial blood samples taken during exposure revealed immediate and rapid uptake of 14C. Clearance of 14C was then gradual over a period of 3 days. Similarly 14C was present in urine and bile immediately following exposure, and clearance paralleled that observed in blood. Guinea pigs fitted with a tracheal cannula and exposed while under anesthesia showed a reduced 14C uptake in blood indicating that most of the 14C MIC uptake in normal guinea pigs occurred from retention of this agent in the upper respiratory tract passages. In exposed guinea pigs 14C was distributed to all examined tissues. In pregnant female mice similarly exposed to 14C MIC, 14C was observed in all tissues examined following exposure including the uterus, placenta, and fetus. While the form of 14C distributed in blood and tissues has not yet been identified, these findings may help to explain the toxicity of MIC or MIC reaction products on organs other than the respiratory tract, as noted by several investigators

1988-06-15

62

Ras p21 and other Gn proteins are detected in mammalian cell lines by [gamma-35S]GTP gamma S binding  

International Nuclear Information System (INIS)

The presence of guanine nucleotide binding proteins in mouse and human cell lines was investigated using [gamma-35S]GTP gamma S and [gamma-32P]GTP. Cell lysate polypeptides were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred to nitrocellulose. Incubation of the nitrocellulose blots with [gamma-35S]GTP gamma S identified 9 distinct GTP-binding polypeptides in all lysates. One of these is the ras oncogene product, p21, as demonstrated by subsequent immunochemical staining of the nitrocellulose blots. We have shown that this procedure provides a sensitive method for detection of p21 in culture cell lines

1989-03-31

63

Ras p21 and other Gn proteins are detected in mammalian cell lines by (gamma-/sup 35/S)GTP gamma S binding  

Energy Technology Data Exchange (ETDEWEB)

The presence of guanine nucleotide binding proteins in mouse and human cell lines was investigated using (gamma-/sup 35/S)GTP gamma S and (gamma-32P)GTP. Cell lysate polypeptides were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred to nitrocellulose. Incubation of the nitrocellulose blots with (gamma-/sup 35/S)GTP gamma S identified 9 distinct GTP-binding polypeptides in all lysates. One of these is the ras oncogene product, p21, as demonstrated by subsequent immunochemical staining of the nitrocellulose blots. We have shown that this procedure provides a sensitive method for detection of p21 in culture cell lines.

Comerford, J.G.; Gibson, J.R.; Dawson, A.P.; Gibson, I.

1989-03-31

64

32P detection in animal and plant samples using Cerenkov  

International Nuclear Information System (INIS)

[en] 32P detection methodology in animal and plant tissue using the Cerenkov effect, liquid scintillation and Geiger-Muller techniques is studied. The Cerenckov effect shows to be more satisflying as to sensitivity, sample preparation, back ground and negligible cost per sample. Comparing the detection by means of Cerenkov effect with that by GM, the relative counting efficiency is about 100 times and 1,5 to 4 comparing to liquid scintillation detection

1977-01-01

65

Genomic 5-methylcytosine determination by 32P-postlabeling analysis  

International Nuclear Information System (INIS)

A simple and sensitive method for the quantitation of 5-methyldeoxycytidine in DNA has been developed by the adaptation of the Randerath 32P-postlabeling technique. Nucleic acids were digested to 3'-monophosphate nucleotides, which were converted to 32P-labeled 3',5'-bisphosphate nucleotides, the 3'-phosphate was cleaved by the action of nuclease P1, and the resultant 5'-[32P]-monophosphate nucleotides were separated by two-dimensional thin-layer chromatography. Less than 1 microgram of DNA was required for the precise quantitation of 5-methyldeoxycytidine content to a detectable limit of 0.01% of the total cytidine residues methylated. The genomic 5-methyldeoxycytidine content may thus be quantitated in tissue samples, small or selective cell populations, senescing or terminally differentiating cells, or DNA from any source. We report here, for the first time, the genomic 5-methyldeoxycytidine content of normal human bronchial epithelial and normal human pulmonary mesothelial cells. The chromatographic separation of all of the normal and some of the rare monophosphate deoxyribonucleotides and ribonucleotides has been characterized. Thus, 5-bromodeoxyuridine and the RNA contamination of DNA or the DNA contamination of RNA can also be quantitated during the same analysis

1986-02-01

66

Deposition of [35S]-carbonyl sulphide to vegetable crops  

International Nuclear Information System (INIS)

Carbonyl sulphide (COS) is the principal form of 35S in the coolant of CO2-cooled nuclear reactors under operational conditions. Measurements were made of the deposition velocity (Vg) of [35S]-COS (on a single-side leaf area basis) to a range of vegetable cultivars under turbulent conditions simulating maximal deposition in the field. The Vg for all the cultivars was -4 m.s-1. Uptake was greatest for brassicas and radishes (mean Vg 4.60-5.79x10-4m.s-1) and least for lettuces, onions and spinach (mean Vg 2.48-3.96x10-4m.s-1). Under illuminated conditions, when the stomata are open, differences in stomatal resistance accounted for -3, about seven times ambient) had no effect on the stomata. The results show that the use of Vg values of [35S]-COS to ryegrass in models to estimate ingested radiation dose following the consumption of green vegetables would increase the margin of safety by overestimating the 35S content of the crop by 30-300%. (author).

1985-01-01

67

Synthesis of [carbonyl-14C]sparfloxacin.  

Science.gov (United States)

A new antimicrobial quinolone, sparfloxacin (5-amino-1-cyclopropyl-7- (cis-3,5-dimethyl-1-piperazinyl)-6,8-difluoro-1,4-dihydro-4-oxoquinoline -3- carboxylic acid, AT-4140; CAS 110871-86-8), was labeled by 14C for studies of disposition and metabolism. Ethyl pentafluoro[carbonyl-14C]benzoylacetate (I) was reacted with ethyl orthoformate, cyclopropylamine and then potassium tert-butoxide to give a quinolone intermediate (IV). A benzylamino derivative (V) obtained by condensation of IV and benzylamine was subject to catalytic hydrogenolysis and hydrolyzed to give the carboxyl derivative (VII), which was condensed with cis-2,6-dimethylpiperazine to form [carbonyl-14C]sparfloxacin. The average yield of 3 preparations was 41.5% and specific activities were 310.8-366.3 MBq (8.4-9.9 mCi)/mmol. Both chemical and radiochemical purities were greater than 99%. PMID:1663353

Kagemoto, A; Negoro, T; Nakao, M; Ochi, T; Chiba, K; Kataoka, M; Sekine, Y

1991-07-01

68

Synthesis of [carbonyl-14C]sparfloxacin.  

UK PubMed Central (United Kingdom)

A new antimicrobial quinolone, sparfloxacin (5-amino-1-cyclopropyl-7- (cis-3,5-dimethyl-1-piperazinyl)-6,8-difluoro-1,4-dihydro-4-oxoquinoline -3- carboxylic acid, AT-4140; CAS 110871-86-8), was labeled by 14C for studies of disposition and metabolism. Ethyl pentafluoro[carbonyl-14C]benzoylacetate (I) was reacted with ethyl orthoformate, cyclopropylamine and then potassium tert-butoxide to give a quinolone intermediate (IV). A benzylamino derivative (V) obtained by condensation of IV and benzylamine was subject to catalytic hydrogenolysis and hydrolyzed to give the carboxyl derivative (VII), which was condensed with cis-2,6-dimethylpiperazine to form [carbonyl-14C]sparfloxacin. The average yield of 3 preparations was 41.5% and specific activities were 310.8-366.3 MBq (8.4-9.9 mCi)/mmol. Both chemical and radiochemical purities were greater than 99%.

Kagemoto A; Negoro T; Nakao M; Ochi T; Chiba K; Kataoka M; Sekine Y

1991-07-01

69

Photoneutron cross sections for 14C  

International Nuclear Information System (INIS)

The photoneutron cross sections for 14C [sigma(?,1n) and sigma(?,2n)] have been measured up to 36 MeV using monochromatic photons. The cross section for the emission of a single neutron displays a very prominent resonance near 15 MeV which appears to decay primarily to the ground state of 13C. The (?,2n) cross section is sharply peaked at 26 MeV and is large compared with those for 12C and 13C. The integrated total photoneutron cross section up to 36 MeV is 126 +- 12 MeV mb (61% of the Thomas-Reiche-Kuhn sum-rule value). The 14C photoneutron cross sections are interpreted in terms of the reaction kinematics and the competition among the particle channels in order to provide information on the isospin properties of the major E1 states in 14C and on the validity of the weak core-coupling model

1985-01-01

70

Effect of iodide on glucose oxidation and 32P incorporation into phospholipids stimulated by different agents in dog thyroid slices  

International Nuclear Information System (INIS)

Since iodide (I-) inhibits TSH stimulation of cAMP formation, which mediates most of the effects of the hormone, it has been assumed that this accounts for the inhibitory action of iodide on the thyroid. However, TSH stimulation of 32P incorporation into phospholipids and stimulation of thyroid metabolism by other agonists, such as carbachol, phorbol esters, and ionophore A23187, is not cAMP mediated. The present studies examined the effect of iodide on stimulation of glucose oxidation and 32P incorporation into phospholipids by TSH and other agonists to determine if the inhibition of cAMP formation was responsible for the action of iodide. Preincubation of dog thyroid slices for 1 h with iodide (10(-4) M) inhibited TSH-, (Bu)2cAMP-, carbachol-, methylene blue-, 12-O-tetradecanoyl phorbol-13-acetate-, ionophore A23187-, prostaglandin E1-, and cholera toxin-stimulated glucose oxidation. I- also inhibited the stimulation by TSH, 12-O-tetradecanoyl phorbol-13-acetate, carbachol, and ionophore A23187 of 32P incorporation into phospholipids. The inhibition was similar whether iodide was added 2 h before or simultaneously with the agonist. I- itself sometimes stimulated basal glucose oxidation, but had no effect on basal 32P incorporation into phospholipids. The effects of iodide on basal and agonist-stimulated thyroid metabolism were blocked by methimazole (10(-3) M). When dog thyroid slices were preloaded with 32PO4 or [1-14C]glucose, the iodide inhibition of agonist stimulation disappeared, suggesting that the effect of iodide involves the transport process. In conclusion, I- inhibited stimulation of glucose oxidation and 32P incorporation into phospholipids by all agonists, indicating that the effect is independent of the cAMP system and that iodide autoregulation does not only involve this system. Oxidation and organification of iodide are necessary for the inhibition

1989-01-01

71

PUKAMS facilities and 14C measurement  

International Nuclear Information System (INIS)

With the prominent advantages of super sensitivity, small sample and high throughput, AMS has been developed rapidly during the past two decades in the world. Its application covers a large variety of fields including earth science, archaeology, physics, environment, material and biological science. Peking University Accelerator Mass Spectrometry (PKUAMS) facilities were established in 1991 and have been put into routine 14C measurement since May, 1993. Since then over 650 samples in diverse application fields have been measured and a series of valuable achievements have been gotten. The authors introduce the facilities systematically and present the methods of 14C measurement in detail

1997-01-01

72

14C-AMS in biomedical research  

International Nuclear Information System (INIS)

[en] Accelerator mass spectrometry has been applied to biomedical research in the past 5 years. While there are a number of facilities involved in biomedical measurements of 26Al and 41Ca, the use of 14C-AMS for tracing organic compounds in vivo and in vitro remains somewhat isolated at our laboratory with a few contributions from two other AMS systems. We present some examples of our research using 14C-AMS. Our successes in measuring low dose kinetics of chemical compounds has implications for pharmacology, toxicology, carcinogenisis, nutrition, drug development and clinical tests

1995-01-01

73

Potential drugs labelled with 14C  

International Nuclear Information System (INIS)

3-Benzylamino-8,9-dimethoxy-5,6-dihydro-imidazo[5,1-a]isoquinoline hydrochloride was labelled with 14C in two different positions: in one case the 14C was built into the position 2 of the imidazole ring, in the other case into the position 3 of the isoquinoline ring. In the first case the mechanism of the halogen-cyano exchange reaction of 1-chloromethyl-5,6-dimethoxy-3,4-dihydro-isoquinoline was investigated by tracer experiments. (author)

1981-01-01

74

Nonenzymatic radiolabeling of protein by 32P-containing nucleotides  

International Nuclear Information System (INIS)

The authors report a nonenzymatic reaction which results in the radiolabeling of proteins by 32P-containing nucleoside triphosphates. The labeling reaction does not require any cofactors, but is greatly enhanced by the presence of alcohols. Even under optimal conditions, less than 1% of the protein molecules undergo modification. This nonspecific labeling represents a serious artifact which may become significant in systems involving low levels of specific labeling, such as photoaffinity labeling. Since the reaction is not limited to specific proteins, this may, however, provide a simple and rapid procedure for the preparation of labeled proteins. (Auth.).

1986-01-01

75

List of 14C data 1975 - 1987  

International Nuclear Information System (INIS)

The results of about 900 14C radiocarbon age determinations performed at the Geographic Institute of the Zurich University and the Institute for Medium Energy Physics of the Federal Institute of Technology, Zurich, in the period from 1975 to 1987 are compiled. The samples stemmed mainly from Switzerland but also from abroad. tabs., 128 refs.

1988-01-01

76

Synthesis of ethanol 14C-1  

International Nuclear Information System (INIS)

[en] The direct reduction by LiAlH4, of a suspension of anhydrous sodium acetate in tetra-hydro-furfuryl-oxy-tetra-hydro-pyran is described. This study has shown that the ethanol thus obtained is impure and that the yields are erratic. On the contrary the reduction of acetyl chloride 1-14C by LiAlH4, in 'diethyl carbitol' leads to ethanol 1-14C of satisfactory purity with a yield of about 71 percent. (author)[fr] Une etude de la reduction directe par LiAlH4, de l'acetate de soude anhydre en suspension dans le tetrahydrofurfuryloxytetrahydropyrane est decrite. Cette etude a montre que l'on obtient de l'ethanol souille d'impuretes, avec un rendement variable. Par contre, la reduction du chlorure d'acetyle 14C-1 par LiAlH4, dans le 'diethyl carbitol' conduit a l'ethanol 14C-1 de purete convenable avec un rendement de l'ordre de 71 pour cent. (auteur)

1958-01-01

77

Binding of phosphatidylcholine-14C to glass.  

UK PubMed Central (United Kingdom)

Lecithin-(14)C was found to bind to Pyrex glass. The percentage bound varied with different organic solvents, being greatest in dioxane solution. Thorough treatment of the glass with dimethyldichlorosilane prevents the binding, which suggests that charge-charge interactions are involved.

Green FA

1969-11-01

78

Binding of phosphatidylcholine-14C to glass.  

Science.gov (United States)

Lecithin-(14)C was found to bind to Pyrex glass. The percentage bound varied with different organic solvents, being greatest in dioxane solution. Thorough treatment of the glass with dimethyldichlorosilane prevents the binding, which suggests that charge-charge interactions are involved. PMID:5348129

Green, F A

1969-11-01

79

Synthesis of [2' - 14C] 8-methoxypsoralen  

International Nuclear Information System (INIS)

[en] The synthesis, the purification as well as checks for purity and identity of [2'-14C] 8-methoxy-psoralen are described. The over-all yields, preparatively as well as radioactively was 16% of the theoretical amount. (author)

1982-01-01

80

Photoproton cross section for 14C  

International Nuclear Information System (INIS)

Using bremsstrahlung, the 14C(?,p) reaction cross section has been measured from threshold to 29 MeV. The integrated cross section up to 30 MeV is 18±3 MeV mb. Above 23.5 MeV, the reported cross section includes a contribution, estimated at 3.5 MeV mb, due to the 14C(?,d) and 14C?,pn) reactions. Essentially the entire 14C(?,p) cross section results from decay of T> dipole states. From knowledge of other decay channels estimates of the cross section, integrated to 30 MeV for the T and T> components of the giant resonance (GDR) of 81 MeV mb and 43 MeV mb are obtained. The splitting of the mean energies of the GDR isospin components is 8.5 MeV. Comparisons with several shell-model calculations are made with the data, and general agreement is found. A comparison of photonuclear absorption cross sections for 12,1314C and 16,17,18 O shows dramatic redistribution of dipole strength as neutrons are added to the core nuclei. 41 refs., 1 tab., 7 figs

1990-01-01

 
 
 
 
81

14C analysis of atmospheric oxidants  

International Nuclear Information System (INIS)

A method for collecting atmospheric samples of formic and acetic acid for 14C analysis has been developed. The method will be applied to determine the relative contribution of fossil fuel and biogenic non-methane hydrocarbons to smog-forming processes. (au).

1996-01-01

82

Synthesis and affinity purification of ?-32P-labeled [?-S]GTP  

International Nuclear Information System (INIS)

A method for the synthesis and purification of guanosine 5'-[?-S]triphosphate labeled with 32P in the ?-position is described. The first step in the synthesis involves the quantitative transfer of 32P/sub i/ from [?-32P]dATP to 5'-GMP catalyzed by GMP kinase. Further incubation of the [?-32P]GDP product with [?-S]GTP and nucleoside diphosphate kinase results in the synthesis of [?-32P][?-S]GTP with a yield of 10 to 18%. The 32P-labeled [?-S]nucleotide is purified by binding to mercury-agarose and eluting with buffer containing ?-mercaptoethanol. Specific incorporation of 32P into the ?-position was demonstrated by treating [?-32P][?-S]GTP with 7% formic acid to remove the ?-thiophosphate and digesting the remaining [?-32P]GDP with nucleotide pyrophosphatase. Although 5'-GMP was released after pyrophosphatase digestion, the only 32P radioactivity detected was as inorganic phosphate.

1983-01-01

83

Neointimal responses 3 months after 32P ?-emitting stent placement  

International Nuclear Information System (INIS)

Purpose: Studies have shown a potential benefit of brachytherapy in preventing restenosis. However, the effects of intravascular radiation on arterial healing have not been well-established. The purpose of this study was to explore the histologic changes following placement of ?-emitting radioactive stents in arteries focusing on intimal responses and endothelialization. Methods and Materials: 3.0-mm ?-emitting 32P stents (6-?Ci and 24-?Ci) were placed in rabbit iliac arteries with nonradioactive stents serving as controls. Animals were euthanized at 3 months and histologic assessment, morphometry, and analysis of endothelialization were performed. Results: The lumen areas of 24-?Ci stents (4.24 ± 0.22 mm2, p 2, p 2). The mean lumen percent stenosis was 11.4 ± 3.0% in the 24-?Ci stents (p 32P ?-emitting stents reduce neointimal growth, but healing is incomplete with poor endothelialization at 3 months. Longer-term studies with complete arterial healing are needed to determine whether there is sustained neointimal inhibition by stent-delivered brachytherapy.

2000-10-01

84

Incorporation of 14C from [14C]phenylalanine into condensed tannin of sorghum grain  

International Nuclear Information System (INIS)

[en] A procedure is described for obtaining condensed tannin from sorghum [Sorghum bicolor (L.) Moench] seeds metabolically labeled from [14C]phenylalanine. The [14C]tannin should be useful in determining the metabolic fate of dietary condensed tannin

1989-01-01

85

Isolation of (-)-menthol-/sup 14/C and (+)-neomenthol-/sup 14/C  

Energy Technology Data Exchange (ETDEWEB)

Uniformly labelled (-)-menthol-/sup 14/C and (+)-neomenthol-/sup 14/C were isolated and purified from the oil of peppermint plants grown in a /sup 14/CO/sub 2/ chamber by preparative high performance liquid chromatography. This procedure is much simpler than previous methods and allows for large amounts of labelled oil to be processed.

Haut, S.A.; Core, M.T. (Philip Morris Research and Development, Richmond, Va. (USA))

1982-01-01

86

Translocation of 14C-assimilates and 14C-herbicides in cow parsnip (Heracleum sphondylium)  

International Nuclear Information System (INIS)

In cow parsnip translocation fo 14CO2, 14C-2,4-D and 14C-2,4,5-T was investigated during four growth phases. It was intended to find out the quantitative correlation between the translocation of phenoxy herbicides and assimilates. The gas exchange used for 14CO2 application is described. Before shoot extension, the root of the plants is the prefered target for assimilates and 2,4-D. Close quantitative correlation was found between the respective 14C activities as regards direction of translocation and radioactivity per dry weight. Similar results were found with 2,4,5-T. At flowering 14C activity moves clearly into the shoot and the inflorescence. At seed maturity, rhizomes and roots take in the 14C activity preferably. Consequently, two growing phases are recommended for optimal weed control by phenoxy compounds, especially 2,4-D: before shooting or after flowering, if sufficient assimilating leaves are left. (orig.)

1975-01-01

87

An improved synthesis of catechol-U-14C  

International Nuclear Information System (INIS)

[en] The high yield, four-step synthesis of catechol-U-14C from benzene-U-14C is described. Intermediates in the preparation include phenol-U-14C and o-methoxyphenol-U-14C [guaiacol-(phenyl-U-14C]. (author)

1982-01-01

88

Effects of ethanol on recombinant rat GABAA receptors. [35S]t-butylbicyclophosphorothionate ([35S]TBPS) binding study  

International Nuclear Information System (INIS)

To determone the roles of the alternatively spliced short and long forms of the ?2 subunit in the effect of ethanol on the GABAA receptor function, picrotoxin-sensitive [35S]t-butylbicyclophosphorothionate ([35S]TBPS) binding was studied in recombinant rat ?1?2?2 and ?6?2?2 recepto expressed in human embryonic kidney 392 cells. Ethanol (10-500 mM) in the absence of added GABA had only minor effects on [35S]TBPS binding irrespective of the ?2 splice variant, its effects being greater in ?6?2?2 than in ?1?2?2 receptors. Ethanol (100 mM) decreased the binding in all four subunit combinations at various concentrations of GABA, again an effect independent of the ?2 variant. The two ?2 variants had different effect on GABA modulation of the binding, with the long ?2 variant decreasing the efficiency of GABA inhibition in ?6?2 receptors and enhancing the biphasic GABA stimulation and inhibition in ?1?2?2 receptors. The findings confirm the importance of the ? subunits in the allosteric interactions between the convulsant binding site and other effector sites, which can be modified only to a minor extent by the tupe of the ?2 splice variant. (au) 31 refs

1995-01-01

89

Essential thrombocythemia treated with oral sup(32)P  

Energy Technology Data Exchange (ETDEWEB)

A 65-year old man was admitted due to protracted gum bleeding after dental surgery. The peripheral blood smear showed thrombocytosis(1,160,000/mm/sup 3/) with bizarre, giant platelets. The paltelet adhesiveness revealed 28% (normal control 31-85%) by Salzman method and the platelet aggregation revealed moderately delayed response to collagen, but normal responsiveness to A.D,P, and no responsiveness to epinephrine, the spleen scan(sup(99m)Tc-NaTcO/sub 4/) showed the finding of splenic infraction. The gum bleeding ceased after transfusion of 8 units of platelet rich plasma, and he was treated with oral sup(32)P(2.5mCi/m/sub 2/) under the diagnosis of essential thrombocythemia. The platelet count returned toward normal 2 months after treatment, and he was in good healthy condition.

Yu, H.D.; Oh, B.S.; Jang, W.S.; Roh, K.H.; Park, B.I.; Cho, M.K. (National Police Hospital, Seoul (Republic of Korea))

1983-04-01

90

Essential thrombocythemia treated with oral sup(32)P  

International Nuclear Information System (INIS)

[en] A 65-year old man was admitted due to protracted gum bleeding after dental surgery. The peripheral blood smear showed thrombocytosis(1,160,000/mm3) with bizarre, giant platelets. The paltelet adhesiveness revealed 28% (normal control 31-85%) by Salzman method and the platelet aggregation revealed moderately delayed response to collagen, but normal responsiveness to A.D,P, and no responsiveness to epinephrine, the spleen scan(sup(99m)Tc-NaTcO4) showed the finding of splenic infraction. The gum bleeding ceased after transfusion of 8 units of platelet rich plasma, and he was treated with oral sup(32)P(2.5mCi/m2) under the diagnosis of essential thrombocythemia. The platelet count returned toward normal 2 months after treatment, and he was in good healthy condition. (Author)

1983-01-01

91

Dosimetry of 32P radiocolloid for treatment of cystic craniopharyngioma  

International Nuclear Information System (INIS)

In Tajrish Shohada Hospital, patients with either cystic craniopharyngiomas or cystic astrocytomas have been treated with 32P radiocolloid by stereotactic procedure. The total activity was prescribed for delivery dose between 200 and 300 Gy to the cyst wall thicknesses of 1-3 mm. In this project, MD-55-2 radiochromic film was utilized to determine the dose distribution around the source. The film dosimetry data were compared with Monte Carlo simulated values calculated with MCNP4C code. In addition, the clinical and dosimetric factors such as the cyst volume, and method of radiocolloid injection were evaluated. The required activity and distribution of radial dose in and out of cyst wall have been investigated.

2007-01-01

92

Endolymphatic treatment with 32P and 131I isotopes  

International Nuclear Information System (INIS)

[en] The method can be applied mainly for the treatment of lymp node metastases of tumours of the lower extremities and melanoma malignum. It is contraindicated in the case of circulation disorders, lymphoedema and after surgical exstirpation or percutaneous irradiation of the lymph nodes. The applied isotopes are: 2 mCi 32P-tri-N-octyl-phosphate plus 0.5 mCi 131I in 7 ml Lipiodol UF contrast material. The same dose is simultaneously injected in the lymph vessels of both feet. The average dose in the lymph nodes is approx. 150000 rad, the radiation exposition of the lungs being around 400 rad. In the I. and II. stage of melanoma malignum the method resulted five-year survival in 90% and 45%, resp. (L.E.)

1981-01-01

93

Photoneutron cross sections for /sup 14/C  

Energy Technology Data Exchange (ETDEWEB)

The photoneutron cross sections for /sup 14/C (sigma(..gamma..,1n) and sigma(..gamma..,2n)) have been measured up to 36 MeV using monochromatic photons. The cross section for the emission of a single neutron displays a very prominent resonance near 15 MeV which appears to decay primarily to the ground state of /sup 13/C. The (..gamma..,2n) cross section is sharply peaked at 26 MeV and is large compared with those for /sup 12/C and /sup 13/C. The integrated total photoneutron cross section up to 36 MeV is 126 +- 12 MeV mb (61% of the Thomas-Reiche-Kuhn sum-rule value). The /sup 14/C photoneutron cross sections are interpreted in terms of the reaction kinematics and the competition among the particle channels in order to provide information on the isospin properties of the major E1 states in /sup 14/C and on the validity of the weak core-coupling model.

Pywell, R.E.; Berman, B.L.; Woodworth, J.G.; Jury, J.W.; McNeill, K.G.; Thompson, M.N.

1985-08-01

94

[Comparative studies of 35S-hemineurine and 35S-thiamine distribution and binding by proteins in the neural structures of the rat brain  

UK PubMed Central (United Kingdom)

35S-Hemineurine penetrated across the blood-brain barrier more rapidly than 35S-thiamin within the first 30 min after intravenous administration. Within 24 hrs content of 35S-thiamin in the neurones exceeded by 34% its concentration in glia and by 200% -- in myelin fraction. 35S-Hemineurine was bound slightly with brain proteins as compared with thiamin and it was removed at the higher rate from brain regions and subcellular structures.

Filippova LB; Rozanov AIa

1980-09-01

95

Metabolic kinetics and biological effects of radiocarbon (14C)  

International Nuclear Information System (INIS)

Radiocarbon 14C is one of the most widespread radionuclides. Increased concentration of anthropogenic 14C in the biosphere is a problem of considerable hygienic and ecological significance. The paper presents the results of comprehensive studies for years on biokinetics of the main 14C compounds, inorganic (Na H14CO3, Na214CO3, K214CO3, Ca14CO3) as well as organic (14C-glucose, 14C-succinic acid, 14C-glucosamine, 14C-glycine, 14C-tryptophane, 14C-valine, 14C-palmitic acid, 14C-stearic acid, 14C-ethyl alcohol, 14C-methyl alcohol, 14C-urea), of 14C as food constituent and of elementary radiocarbon. The 14C toxicity is investigated for both acute and chronic small doses received by animals. The nuclide's genetic efficiency is assessed. Based on research evidence, the accumulation multiple and equilibrium time are estimated for long-term 14C intake by humans. The data may be used for setting genetic standards and estimating an anthropogenic increase of 14C concentration in the environment. The hazard of anthropogenic radiocarbon is assessed with allowance for further development of nuclear power industry. (author)

1988-01-01

96

The enzymatic preparation of [?-32P] ATP with high specific activity  

International Nuclear Information System (INIS)

A modified procedure for preparing [?-32P]ATP is described with which [?-32P]GTP is used as phosphate donor instead of [?-32P]ATP. The specific activity of the resulted [?-32P]ATP in other experiments is raised greatly as to 148 PBq/mol or more, without decreasing the yield of [?-32P]ATP which is over 93% based on 32Pi. The specific activity is measured by HPLC and the factors that affect the specific activity of [?-32P]ATP are discussed

1997-01-01

97

Studies on 14C-extractable residue, 14C-bound residue and mineralization of 14C-labeled metsulfuron-methyl in soils  

International Nuclear Information System (INIS)

The purpose of the present study was to investigate 14C-extractable residue (14C-ER), 14C-bound residue (14C-BR) and mineralization of 14C-labeled metsulfuron-methyl in soils. The main factors affecting 14C-BR formation and the distribution pattern of 14C-BR in humus were also discussed in details. The results were as follows: (1) The total 14C-ER content of 14C-metsulfuron-methyl in seven kinds of soil was positively related to soil pH and negatively related to clay content significantly during the initial 20 days of incubation, however, only positively relation to soil pH was found after 30 days of incubation. Moreover, the half-life values of 14C-metsulfuron-methyl parent compound derived from 14C-ER in Soils tested ranged from 13.3 to 66.6 days. The degradation rate conefficient ? (day-1) was negatively related to soil pH. (2) The 14C-BR content of 14C-metsulfuron-methyl in soils was significantly negatively related to soil pH and positively related to the clay content during the initial 20 days of incubation, but only was significantly negatively related to soil pH after 30 days incubation. Again, the soil pH was found to be the dominant factor affecting BR formation of 14C-metsulfuron-methyl among the basic properties of soil. The maximum content of 14C-BR of 14C-metsulfuron-methyl in the soils accounted for 19.3%-52.6% of applied amount. (3) During the whole periods of the incubation, the 14C-BR of 14C-metsulfuron-methyl in the soils was found to be mainly associated with fulvic acid and humin. The relative percentage of 14C-BR in fulvic acid was higher than in humin, while the content of the 14C-BR distributed in humic acid only account for less than 1% of applied amount. Therefore, fulvic acid played an important role in the process of BR formation of 14C-metsulfuron-methyl. (4) About 12.9%-27% of applied amount of 14C-metsulfuron-methyl was mineralized to 14CO2 through opening 14C-triazine ring in soils tested after 180 days of the incubation. It was difficult for 14C-metsulfuron-methyl to mineralize to 14CO2 in an alkaline soil. (authors)

2004-01-01

98

Synthesis of 14C analogue of 1,2-diaryl-[2-14C]-pyrroles  

International Nuclear Information System (INIS)

Three 1,2-diaryl pyrroles selective COX-2 inhibitors, 2-(4-fluorophenyl)-5-methyl-1-(4-methylsulfonyl-phenyl)-1H pyrrole, 2-(4-fluorophenyl)-1- [4-(methylsulfonyl) phenyl]-1H-pyrrole and 4-[2-(4-fluorophenyl)-1H-pyrrol-1-yl]benzenesulfon-amide, all three labeled with 14C in the 2-position were prepared from para-fluoro-benzaldehyde-[carbonyl-14C]. (author)

2007-01-01

99

Distribution of radioactive phosphorus (32 P) in sheep tissue  

International Nuclear Information System (INIS)

[en] Twelve male lambs (Corriedale) were separated in two groups according to their average age (114 days and 208 days). The animals of each group were selected in twos according to weight to provide duplicates. After a 7 days period of adaptation, during which time the lambs received anunrestricted mineral supplement, they were given intravenous injections of 1 ml of Na2H32PO4. The amount of radioactivity administered varied with time; from 500 ?ci to 225.9 ?ci for the 6 younger animals, and from 500 ?ci to 87.5 ?ci for the 6 older ones. At 8, 16, and 24 hours after the injections, the animals were anesthetized and sacrificed by bleeding. As well as the serum and blood, pieces of tongue, brain, heart, diaphragm spleen, kidney, liver and bone were collected. These tissues were dried, incinerated and the extracts obtained with H2SO4 counted by Cerenkov radiation in a liquid scintillation counter. The level of inorganic phosphorus in the serum was determined with material obtained the day before the experiment. The younger animals had lower levels of serum inorganic phosphorus and retained higher levels of 32P on their tissues. The isotope was found in a decreasing order in the following tissues: liver, kidney, spleen heart, and tongue. The brain presented the least retention. The order of magnitude by which the tissues retained the isotope was not influenced by the age of the animals, by the experimental period, or by the level of serum inorganic phosphorus. The amount of phosphorus retained by the tissues was affected not only by the age of the animals, but also by the level of phosphorus in the blood and, for some tissues, by the experimental period[pt] Doze carneiros, machos da raca Corriedale foram separados em dois grupos conforme a idade media (114 dias e 208 dias). Os animais de cada grupo foram sorteados dois a dois conforme o peso e constituiram as repeticoes. Apos um periodo de adaptacao de 7 dias, ocasiao em que continuavam recebendo suplementacao mineral a vontade, eram submetidos a uma injecao endovenosa de 1 ml de Na2H32PO4. No volume aplicado a radioatividade variou com o decorrer do tempo: de 500 ?ci a 225,9 ?ci para os 6 animais da primeira fase, e de 500 ?ci a 87,5 ?ci para os 6 animais da segunda fase. Apos 8, 16 e 24 horas da aplicacao da injecao, os animais foram anestesiados e sacrificados por sangria total. Alem do soro e sangue, foram coletados fragmentos de lingua, cerebro, coracao, diafragma, baco, rim, figado e osso. Os tecidos foram secos, incinerados, e os extratos obtidos com acido sulfurico levado ao cintilador liquido e sua radioatividade detectada atraves da radiacao Cerenkov. O teor serico de fosforo inorganico dos animais foi determinado com material obtido na vespera do experimento. Os animais mais novos apresentaram menor teor serico de fosforo inorganico e maior retencao de 32P em seus tecidos. A retencao do 32P nos tecidos decresceu na seguinte ordem: figado, rim, baco, coracao e lingua. O cerebro sempre apresentou menor retencao. Essa ordem nao foi influenciada pela idade dos animais, pelo tempo e nem pelo serico do fosforo inorganico. O montante do fosforo retido pelos tecidos foi influenciado nao so pela idade dos animais mas tambem pelo teor de fosforo no sangue e para alguns tecidos, tambem pelo tempo experimental

1973-01-01

100

The synthesis of HIV reverse transcriptase inhibitors [14C]L-697,661, [14C]L-697,639, [14C]L-702,007  

International Nuclear Information System (INIS)

The synthesis of three carbon-14 labeled reverse transcriptase inhibitors has been accomplished by elaboration of a common intermediate, 5-ethyl-6-methyl-3-amino-2-(1H)-[4-14C]-pyridinone (7). Ethyl [1-14C]formate, prepared by esterification of sodium [14C]formate, was combined with 2-pentanone under basic conditions to afford 3-[14C]carboxyaldehyde-2-pentanone sodium salt (2). The pyridinone ring was constructed by condensation of 2 with nitro acetamide 4. Reduction of the nitro group afforded 5-ethyl-6-methyl-3-amino-2-(1H)-[4-14C]-pyridinone (7)(specific activity 54 mCi/mmol). Subsequent alkylation of 7 provided the desired reverse transcriptase inhibitors [14C]L-697,661, [14C]L-697,639, and [14C]L-702,007. (Author)

1996-01-01

 
 
 
 
101

An overview of DNA fingerprinting with 32P nucleotides  

International Nuclear Information System (INIS)

The DNA probes radiolabeled with 32P, a primary tool employed by researchers in the life sciences for > 20 yr, are used by private companies, state-run laboratories, and the FBI to generate autoradiographs displaying the unique banding patterns that constitute the DNA fingerprint. The ability to identify an individual or animal from a biological sample has profound implications. Unidentified bodies, unrecognizable remains, and missing children can be tested and the DNA fingerprint compared to those of family members for positive identification. Paternity can be established before a child's birth. Immigration disputes can easily be resolved. Other uses include pedigree determination and testing for cell-line cross-contamination. Using a DNA fingerprint to determine the guilt or innocence of an individual allegedly involved in a violent crime is very controversial and has great legal and moral implications for society. Forensic laboratories have been challenged to ensure a level of quality control and quality assurance consistent with the weight given to these tests when used as evidence in a court of law.

1992-01-01

102

Fractionation of applied 32P labeled TSP in calcareous soils  

International Nuclear Information System (INIS)

Calcareous dark brown red soil (calcixerollic xerochrept) from northern Syria was used in a pot experiment to study the fate of triple super phosphate fertilizer (TSP) with and without a crop (Local durum wheat (Triticum turgidum L. group durum (Desf)) c v. Bohouth). The soil received 17?g P/g soil of 32 P labeled TSP, and samples were collected from soils and plants at successive dates. Soil inorganic P was ?94% of total soil P, with only 50-80% being soluble. Calcium phosphate compounds were the dominant fraction (?68%) of the soluble inorganic soil P followed by occluded iron phosphate (?48%) and all other fractions were ?9%. Isotopic measurements showed that ? 50% of fertilizer P was nonexchangeable within 2 days, and TSP values in each fraction of soil inorganic P fluctuated in relatively similar proportions to the concentrations of fractions in soil. Available P (soil and TSP) in cropped soil was more than that in the uncropped soil, and plants had no effect on the distribution of P from fertilizer amongst the different P fractions. (author)

2005-01-01

103

An overview of DNA fingerprinting with sup 32 P nucleotides  

Energy Technology Data Exchange (ETDEWEB)

The DNA probes radiolabeled with {sup 32}P, a primary tool employed by researchers in the life sciences for > 20 yr, are used by private companies, state-run laboratories, and the FBI to generate autoradiographs displaying the unique banding patterns that constitute the DNA fingerprint. The ability to identify an individual or animal from a biological sample has profound implications. Unidentified bodies, unrecognizable remains, and missing children can be tested and the DNA fingerprint compared to those of family members for positive identification. Paternity can be established before a child's birth. Immigration disputes can easily be resolved. Other uses include pedigree determination and testing for cell-line cross-contamination. Using a DNA fingerprint to determine the guilt or innocence of an individual allegedly involved in a violent crime is very controversial and has great legal and moral implications for society. Forensic laboratories have been challenged to ensure a level of quality control and quality assurance consistent with the weight given to these tests when used as evidence in a court of law.

Pappas, G.G.

1992-01-01

104

Synthesis of dexamethasone-4-/sup 14/C  

Energy Technology Data Exchange (ETDEWEB)

The bismethylenedioxy (BMD) derivative of dexamethasone 2 was silylated with trimethylchlorosilane and imidazole in dimethylformamide to give the 11..beta..-trimethylsilyloxy BMD derivative 3. The ..delta../sup 1/-double bond in 3 was hydrogenated over 5% palladium on carbon to give the ..delta../sup 4/-3-oxo steroid 4. Oxidation of 4 with potassium permanganate-sodium metaperiodate gave the seco-acid 5 which on subsequent treatment with acetic anhydride; sodium acetate and triethylamine gave the enol-lactone 6. The enol-lactone 6 was reacted with /sup 14/C-methylmagnesium iodide to give an adduct 7a which on heating at reflux with lithium 2,6-di-t-butylphenoxide in dioxane gave the ..delta../sup 4/-3-oxo derivative 8. Compound 8 was heated at reflux with m-iodylbenzoic acid and diphenyl diselenide in toluene to give the ..delta..sup(1,4)-3-oxo steroid 9. The protecting BMD and silyl groups were removed in a single step by reaction with aqueous trifluoroacetic acid containing 2N hydrochloric acid at room temperature to give dexamethasone-4-/sup 14/C 10.

Rao, P.N.; Cessac, J.W.; Hill, K.A. (Southwest Foundation for Research and Education, San Antonio, TX (USA))

1982-10-01

105

Synthesis of dexamethasone-4-14C  

International Nuclear Information System (INIS)

The bismethylenedioxy (BMD) derivative of dexamethasone 2 was silylated with trimethylchlorosilane and imidazole in dimethylformamide to give the 11?-trimethylsilyloxy BMD derivative 3. The ?1-double bond in 3 was hydrogenated over 5% palladium on carbon to give the ?4-3-oxo steroid 4. Oxidation of 4 with potassium permanganate-sodium metaperiodate gave the seco-acid 5 which on subsequent treatment with acetic anhydride; sodium acetate and triethylamine gave the enol-lactone 6. The enol-lactone 6 was reacted with 14C-methylmagnesium iodide to give an adduct 7a which on heating at reflux with lithium 2,6-di-t-butylphenoxide in dioxane gave the ?4-3-oxo derivative 8. Compound 8 was heated at reflux with m-iodylbenzoic acid and diphenyl diselenide in toluene to give the ?sup(1,4)-3-oxo steroid 9. The protecting BMD and silyl groups were removed in a single step by reaction with aqueous trifluoroacetic acid containing 2N hydrochloric acid at room temperature to give dexamethasone-4-14C 10. (author)

1982-01-01

106

The Survival Time of DF32P-labelled Erythrocytes in Adult Male Mink  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The study indicated that intravenously administered DF32P was a suitable label for mink erythrocytes. The mean survival time of erythrocytes of 29 adult male mink labelled with diisopropyl phosphorofluoridate32P(DF32P) was 92.2 ± 4.1 (SEM) days. Three colour types were tested: standard dark, pastel ...

Fletch, S. M.; Robinson, G. A.; Karstad, L. H.

107

Bioconversion of alpha-[14C]zearalenol and beta-[14C]zearalenol into [14C]zearalenone by Fusarium roseum 'Gibbosum'.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Cultures of Fusarium roseium 'Gibbosum' on rice were treated with [14C]zearalenone, alpha[14C]zearalenol, or beta-[14C]zearalenol to determine whether a precursor-product relationship exists among these closely related fungal metabolites. Culture extracts were purified by silica gel column chromatog...

Richardson, K E; Hagler, W M; Hamilton, P B

108

Synthesis of (14C-4-pyrimidinyl)-bensulfuron-methyl  

International Nuclear Information System (INIS)

(14C-4-pyrimidinyl)-bensulfuron-methyl was synthesized from 14C-sodium cyanide with 6 steps. First, malonic acid (14C-carboxyl) was prepared, then changed to ethyl (14C-carboxyl) malonate by diazoethane. It reacted with guanidine nitrate, forming 14C-2-amino-4,6-dihydroxy pyrimidine, which changed into 14C-2-amino-4,6-dimethyloxy-pyrimidine. This product reacted with methyl-2-(methylene-sulfonyl isocyanate) benzoate, forming (14C-4-pyrimidinyl)-bensulfuron-methyl. The radiochemical yield was 14.6%, radiochemical purity was more than 95%, and the specific activity was 28.9 kBq/mg

1998-01-01

109

Brachytherapy on restenosis. 32P radioisotope in animal model  

International Nuclear Information System (INIS)

Despite a notorious decline in age-adjusted death rates for cardiovascular pathologies, coronary artery disease still remains as the main cause of mortality above the age of 40 in men and 60 in women. More than 25% of death in persons over the age of 35 are due to coronary disease. In about 50% of men and 30% of women, the first manifestation of the disease is an acute myocardial infarction and 10% a sudden cardiac death. In Argentina it is estimated that in 1998 about 100.000-115.000 people suffered as first manifestation of coronary illness a myocardial acute infarct. Angioplasty has an important and well established site in the treatment of the coronary illness and restenosis represents the principal complication of this method for myocardial re-vascularization. About a 35-40% of treated arteries present restenosis within the first six month the intervention with the concomitant need of re-interventions, re-hospitalizations, by-pass surgery, work discontinuity and the high cost for the health system. A number of drugs were tested as anti-restenosis: anticoagulants, aspirin, antispasmodics and lipid-lowering agents but none was clearly efficient; also, experimental studies in which intravascular irradiation with different source types and energies, radiation doses and doses rate to prevent restenosis was utilized; however, there is no consensus in many aspects of this intravascular brachytherapy. The first step in this work was to induce the experimental model in rabbits. Afterwards, by means of the balloon methodology and stent implantation, brachytherapy experiments were carried out to evaluate the biological effect on different layers of arteries, with different Doses using a beta particle emitting radioisotope (32P). The arteriosclerotic lesions were induced in New Zealand rabbits through the administration of a diet with high cholesterol content. Angioplastic interventions on femoral arteries were done with balloon methodology and controlled by fluoroscopy when rabbits had reached 3,0-3,5 Kg body weight. Studies were done with different doses (10 and 20 Gy) of beta ionizing radiation (32P sources) administered with balloon dilatation. Animals were sacrificed at the same time after irradiation; vasodilation, edema, hemorrhages, mononuclear infiltrate in the periadventitial tissue and other biological changes were analyzed and compared with control. Evaluation of Absorbed dosis and Dosis Equivalent on different tissues were done by Montecalo and appropriate equations. Evaluation of effects of ionizing radiation into the different layers of the arterial wall (i.e. histological percent stenosis area and number of neointimal cell layer), microscopic observation and computational count with the adequate software indicated that imflamatory elementsaccumulation and neointimal cell proliferation (demonstrated through PCNA+ and apoptotic cells detection) were milder in irradiated rabbits and that the inhibition of cell proliferation was proportional to the applied Absorbed dose. The ionizing radiation produce inhibitory effect on restenosis in New Zealand rabbits. Results obtained may open the possibility to apply this technique in human patients in Argentina. (author)

2000-01-01

110

Brachytherapy on restenosis. {sup 32}P radioisotope in animal model  

Energy Technology Data Exchange (ETDEWEB)

Despite a notorious decline in age-adjusted death rates for cardiovascular pathologies, coronary artery disease still remains as the main cause of mortality above the age of 40 in men and 60 in women. More than 25% of death in persons over the age of 35 are due to coronary disease. In about 50% of men and 30% of women, the first manifestation of the disease is an acute myocardial infarction and 10% a sudden cardiac death. In Argentina it is estimated that in 1998 about 100.000-115.000 people suffered as first manifestation of coronary illness a myocardial acute infarct. Angioplasty has an important and well established site in the treatment of the coronary illness and restenosis represents the principal complication of this method for myocardial re-vascularization. About a 35-40% of treated arteries present restenosis within the first six month the intervention with the concomitant need of re-interventions, re-hospitalizations, by-pass surgery, work discontinuity and the high cost for the health system. A number of drugs were tested as anti-restenosis: anticoagulants, aspirin, antispasmodics and lipid-lowering agents but none was clearly efficient; also, experimental studies in which intravascular irradiation with different source types and energies, radiation doses and doses rate to prevent restenosis was utilized; however, there is no consensus in many aspects of this intravascular brachytherapy. The first step in this work was to induce the experimental model in rabbits. Afterwards, by means of the balloon methodology and stent implantation, brachytherapy experiments were carried out to evaluate the biological effect on different layers of arteries, with different Doses using a beta particle emitting radioisotope ({sup 32}P). The arteriosclerotic lesions were induced in New Zealand rabbits through the administration of a diet with high cholesterol content. Angioplastic interventions on femoral arteries were done with balloon methodology and controlled by fluoroscopy when rabbits had reached 3,0-3,5 Kg body weight. Studies were done with different doses (10 and 20 Gy) of beta ionizing radiation ({sup 32}P sources) administered with balloon dilatation. Animals were sacrificed at the same time after irradiation; vasodilation, edema, hemorrhages, mononuclear infiltrate in the periadventitial tissue and other biological changes were analyzed and compared with control. Evaluation of Absorbed dosis and Dosis Equivalent on different tissues were done by Montecalo and appropriate equations. Evaluation of effects of ionizing radiation into the different layers of the arterial wall (i.e. histological percent stenosis area and number of neointimal cell layer), microscopic observation and computational count with the adequate software indicated that imflamatory elementsaccumulation and neointimal cell proliferation (demonstrated through PCNA+ and apoptotic cells detection) were milder in irradiated rabbits and that the inhibition of cell proliferation was proportional to the applied Absorbed dose. The ionizing radiation produce inhibitory effect on restenosis in New Zealand rabbits. Results obtained may open the possibility to apply this technique in human patients in Argentina. (author)

Bergoc, R.; Rivera, E.; Cocca, C.; Martin, G.; Cricco, G. [Buenos Aires Univ. (Argentina). School of Pharmacy and Biochemistry; Croci, M.; Guzman, L.

2000-05-01

111

Bioconversion of. cap alpha. -(/sup 14/C)Zearalenol and. beta. -(/sup 14/C)Zearalenol into (/sup 14/C)Zearalenone by Fusarium roseum Gibbosum  

Energy Technology Data Exchange (ETDEWEB)

Cultures of Fusarium roseum Gibbosum on rice were treated with (/sup 14/C)zearalenone, ..cap alpha..-(/sup 14/C)zearalenol, or ..beta..-(/sup 14/C)zearalenol to determine whether a precursor-product relationship exists among these closely related fungal metabolites. Culture extracts were purified by silica gel column chromatography and fractionated by high-pressure liquid chromatography, and the level of radioactivity was determined. Within 7 days, the ..beta..-(/sup 14/C)zearalenol was converted to zearalenone, and no residual ..beta..-(/sup 14/C)zearalenol was detectable. Most of the ..cap alpha..-(/sup 14/C)zearalenol added was also converted into zearalenone within 14 days. In cultures treated with (/sup 14/C)zearalenone, no radioactivity was noted in any other components.

Richardson, K.E.; Hagler, W.M. Jr.; Hamilton, P.B.

1984-06-01

112

Convenient one-pot synthesis of benzoquinone-[U-14C] and hydroquinone-[U-14C  

International Nuclear Information System (INIS)

[en] Benzoquinone-[U-14C] was synthesized in 90% yield from benzene-[U-14C] via a three step sequence. Conditions were developed which allowed this conversion to be accomplished in a one pot closed system. The benzoquinone-[U-14C] thus obtained could be reduced to hydroquinone-[U-14C] in quantitative yield. Preparation of benzene-[U-14C] is also described. (author)

1986-01-01

113

[14C]Lignin-labeled lignocelluloses and 14C-labeled milled wood lignins  

International Nuclear Information System (INIS)

[en] This paper discusses lignin which is synthesized in vascular plants by way of a branching sequence of reactions during which CO2 is converted first to shikimic acid. Conversion of two amino acids is described. The authors summarize methods used to prepare, characterize, and use two types of specifically 14C- labeled lignins

1988-01-01

114

Synthesis of. beta. -sitosterol-/sup 14/C  

Energy Technology Data Exchange (ETDEWEB)

The method of synthesis of ..beta..-sitosterol-4-/sup 14/C starting with the enolactone 4-oxa-5-sitosten-3-one is discussed. Methyl-/sup 14/C magnesium iodide is utilized for the introduction of the label. The authors selected this method for the isolation of ..beta..-sitosterol-/sup 14/C, introducing a series of changes into the original method. The authors discuss obtaining sitostenone, the ketoacid of sitostenone, the enol-lactone of the ketoacid of sitostenone, sitostenone-4-/sup 14/C (by different methods), the enol-acetate of sitostenone-/sup 14/C, and ..beta.. sitosterol-4-/sup 14/C.

Askinazi, B.Z.; Kivokurtseva, L.N.; Bobrova, N.S.; Kozarinskaya, N.Ya.

1986-08-01

115

An experimental study of targeting therapy with 35S-SZ39 against glioma  

International Nuclear Information System (INIS)

Objective: To prepare a pure ?-emitting immunoradiotherapeutics agent 35S-MAb SZ39, and validate its special therapeutic efficacy against glioma. Methods: MAb SZ39 was labelled with 35S using a carbodiimide method. Using 35S-nIgG, 35S + MAb SZ39 and sustained 35S as control agents, and human brain glioma cell line SHG-44 as target cell, the injury rate and 50% inhibitory concentration of 35S-MAb SZ39 were evaluated with MTT method. 35S-MAb SZ39 and its control agent 35S-nIgG or PBS were i.p. injected into glioma-bearing nude mice. The tumor inhibitory rate (I) was determined according to the formula: I = [1-(TV35S-MAb/TVPBS)] x 100% (TV: tumor volume). Flowcytometry was used to analyse the cell cycle of glioma after treatment. Results: 35S-MAb SZ39 had a strong cytotoxic effect to glioma cells with 4.2-fold and 4.0-fold more toxic than 35S-nIgG and 35S + MAb SZ39, as strong as the sustained 35S control group. Tumor growth blocking for one week was obtained with 103.6 MBq 35S-MAb SZ39 treatment. The inhibitory rate was 50% 26 days after 35S-MAb SZ39 administration. DNA synthesis of glioma cells was inhibited, cells were accumulated in S period and the road to G1 period was blocked. There was a trend of cell cycle synchronization. No obvious toxicity was found on bone marrow while 35S-MAb SZ39 made the glioma growth block. Conclusions: 35S-MAb SZ39 has a strong selective injurious effect on glioma and is of good prospect to be an immunoradiotherapeutics agent

1999-01-01

116

Synthesis and affinity purification of. beta. -/sup 32/P-labeled (. gamma. -S)GTP  

Energy Technology Data Exchange (ETDEWEB)

A method for the synthesis and purification of guanosine 5'-(..gamma..-S)triphosphate labeled with /sup 32/P in the ..beta..-position is described. The first step in the synthesis involves the quantitative transfer of /sup 32/P/sub i/ from (..gamma..-/sup 32/P)dATP to 5'-GMP catalyzed by GMP kinase. Further incubation of the (..beta..-/sup 32/P)GDP product with (..gamma..-S)GTP and nucleoside diphosphate kinase results in the synthesis of (..beta..-/sup 32/P)(..gamma..-S)GTP with a yield of 10 to 18%. The /sup 32/P-labeled (..gamma..-S)nucleotide is purified by binding to mercury-agarose and eluting with buffer containing ..beta..-mercaptoethanol. Specific incorporation of /sup 32/P into the ..beta..-position was demonstrated by treating (..beta..-/sup 32/P)(..gamma..-S)GTP with 7% formic acid to remove the ..gamma..-thiophosphate and digesting the remaining (..beta..-/sup 32/P)GDP with nucleotide pyrophosphatase. Although 5'-GMP was released after pyrophosphatase digestion, the only /sup 32/P radioactivity detected was as inorganic phosphate.

Reeve, A.E.; Huang, R.C.

1983-04-01

117

Enzymatic method for the synthesis of [14C]pyridoxal 5-phosphate from [14C]pyridoxine  

International Nuclear Information System (INIS)

A new enzymatic method for the synthesis of [14C]pyridoxal 5-phosphate is presented. [14C]Pyridoxal 5'-phosphate was synthesized from [14C]pyriodoxine through the successive actions of pyridoxal kinase and pyrdoxamine 5'-phosphate oxidase in a reaction mixture containing ATP, [14C]pyridoxine, and both enzymes. [14C]Pyridoxal 5'-phosphate was isolated by omega-aminohexyl-Sepharose 6B column chromatography. The overall yield of the product was more than 60%, starting from 550 nmol of [14C]pyridoxine. The radiochemical purity of the products, as determined by thin-layer and ion-exchange chromatography, was greater than 98%.

1986-01-01

118

S-adenosyl-L-(l-/sup 14/C)-homocysteine  

Energy Technology Data Exchange (ETDEWEB)

S-Adenosyl-L-(1-/sup 14/C)-homocysteine was prepared from commercially available L-(1-/sup 14/C)-methionine by conversion first to S-benzyl-L-(1-/sup 14/C)-homocysteine which upon treatment with sodium in liquid ammonia gave the disodium salt of L-(1-/sup 14/C)-homocysteine. Reaction of this sodium salt with 5'-O-tosyladenosine gave the title compound.

Thomas, H.J.; Montgomery, J.A.

1987-12-01

119

S-adenosyl-L-(l-14C)-homocysteine  

International Nuclear Information System (INIS)

S-Adenosyl-L-(1-14C)-homocysteine was prepared from commercially available L-(1-14C)-methionine by conversion first to S-benzyl-L-(1-14C)-homocysteine which upon treatment with sodium in liquid ammonia gave the disodium salt of L-(1-14C)-homocysteine. Reaction of this sodium salt with 5'-O-tosyladenosine gave the title compound. (author)

1987-01-01

120

Synthesis of /sup 14/C-Bucromarone succinate and hydrochloride  

Energy Technology Data Exchange (ETDEWEB)

/sup 14/C-Bucromarone, 2-(4-(3-N,N dibutylamino propoxy) 3,5-dimethyl benzoyl) chromone, was synthesized from (U-/sup 14/C) oxalic acid. The labelling takes place at the first step of the synthesis, giving /sup 14/C-Bucromarone succinate (specific activity 7.45 mCi/mmol) and /sup 14/C-Bucromarone hydrochloride (specific activity 7.5 mCi/mmol).

Nicolas, C.; Verny, M.; Maurizis, J.-C.; Payard, M.; Faurie, M.

1986-08-01

 
 
 
 
121

Design and bioevaluation of a {sup 32}P-patch for brachytherapy of skin diseases  

Energy Technology Data Exchange (ETDEWEB)

The purpose of this study was to design and evaluate a {sup 32}P patch for brachytherapy of skin diseases. We employed Phosphoric-{sup 32}P-acid and Chromic {sup 32}P-phosphate in combination with natural rubber or silicone to produce the patches. Stability studies in vitro to evaluate the leakage of radioactivity, autoradiographic studies to evaluate homogeneity and shielding, as well as therapeutic efficacy in an animal model of skin cancer of the selected {sup 32}P patch were performed. The {sup 32}P-silicone-patch demonstrated its safety for external application. Tumor growth was arrest and complete regressions of tumors were seen in some other cases with 40 Gy applied in a single-dose scheme. In conclusion, the {sup 32}P-silicone-patch is easy to prepare and use in the treatment of skin diseases.

Salgueiro, M.J. [Radioisotope Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires (Argentina)], E-mail: jsalgueiro@ffyb.uba.ar; Duran, H. [Radiobiology Department, National Atomic Energy Commission (CNEA) Buenos Aires, CONICET and School of Science and Technology, University of San Martin, San Martin (Argentina); Palmieri, M. [Biodiversity and Experimental Biology Department, School of Exact and Natural Sciences, University of Buenos Aires, Buenos Aires (Argentina); Pirchio, R. [Dosimetry Department, National Atomic Energy Commission (CNEA) Buenos Aires (Argentina); Nicolini, J.; Ughetti, R. [Research and Development Department, Laboratorios Bacon SAIC (Argentina); Papparella, M.L. [Pathology Department, School of Dentistry, University of Buenos Aires, Buenos Aires (Argentina); Casale, G. [Research and Development Department, Laboratorios Bacon SAIC (Argentina); Zubillaga, M. [Radioisotope Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires (Argentina)

2008-03-15

122

Structure of 26Na from the 14C(14C, d) reaction  

International Nuclear Information System (INIS)

The 14C(14C, d) reaction at 22 MeV was used to study Tz=2 26Na. Charged particles were detected with a Si detector telescope at 0 deg., and ? transitions in coincidence were detected with an array of three Compton-suppressed 'clover' detectors and seven Compton-suppressed single Ge crystals. The Deuteron-? and d-?-? coincidence data were analyzed to study the structure of 26Na. New levels were found, and precise energies and ?-decay patterns were determined for many states previously observed in charge-exchange reactions. Candidates were observed for the 1+ state missing in ? decay. Mixing ratios were determined for some ? transitions from angular distribution information. There is reasonable agreement with the model based on the universal s-d shell (USD) interaction

2006-01-01

123

Production of [14C]patulin by Penicillium patulum.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Factors affecting the production of [14C]patulin from [1-14C]acetate by replacement cultures of Penicillium patulum have been investigated. Incorporation of [1-14C]acetate into patulin reached a maximum with 6- to 8-day-old cultures incubated at 28 degrees C for 8 h in a replacement medium containin...

Nip, W K; Chu, F S

124

An easy enzymatic method for [14 C]trehalose preparation  

International Nuclear Information System (INIS)

[en] An easy and practical method for [14 C]trehalose preparation is described. By incubating [14 C]glucose, ?-glucose-1-phosphate and trehalose phosphorylase from Euglena gracilis the disaccharide was obtained with a yield ranging from 33% to 75% depending on the specific activity of the [14 C]glucose utilized. Trehalose was purified by paper chromatography. (author)

1993-01-01

125

14C AMS dating Yongcheon cave  

International Nuclear Information System (INIS)

[en] The biggest island in South Korea is Jeju Island, which lies 80 km south of the mainland and has one shield volcano, Mt. Halla. The volcanic island and its lava tubes were added to the world heritage list by UNESCO in 2007. Among the many lava tubes on the island, a unique cave had been accidentally found in 2005 while some workers were replacing a telephone pole. Until the discovery, it had been completely isolated from the outside by naturally-built sand blocks. Yongcheon cave is a lime-decorated lava tube showing both the properties of a volcanic lava tube and a limestone cave. This cave, about 3 km in length, is acknowledged to be the best of this type in the world and includes a large clean-water lake, lava falls, and richly developed speleothems inside it. Even though there is archaeological evidence from well preserved pottery that ancient people entered this place, the preservation of artifacts was ensured by a geological change that made later entrance difficult. We have collected charcoal samples scattered around the cave and dated them using AMS. Ages were in the range of ca. 1570-1260 BP (A.D. 340–880) and this corresponds to the Ancient Three Kingdoms and the Unified Silla era in Korean history. The 14C AMS measurement results presented in this paper on wood charcoal provide precise dates which will be very useful not only to clarify the nature of human activities in this cave but also to provide reference dates when comparing other dating methods.

2013-01-01

126

14C AMS dating Yongcheon cave  

Science.gov (United States)

The biggest island in South Korea is Jeju Island, which lies 80 km south of the mainland and has one shield volcano, Mt. Halla. The volcanic island and its lava tubes were added to the world heritage list by UNESCO in 2007. Among the many lava tubes on the island, a unique cave had been accidentally found in 2005 while some workers were replacing a telephone pole. Until the discovery, it had been completely isolated from the outside by naturally-built sand blocks. Yongcheon cave is a lime-decorated lava tube showing both the properties of a volcanic lava tube and a limestone cave. This cave, about 3 km in length, is acknowledged to be the best of this type in the world and includes a large clean-water lake, lava falls, and richly developed speleothems inside it. Even though there is archaeological evidence from well preserved pottery that ancient people entered this place, the preservation of artifacts was ensured by a geological change that made later entrance difficult. We have collected charcoal samples scattered around the cave and dated them using AMS. Ages were in the range of ca. 1570-1260 BP (A.D. 340–880) and this corresponds to the Ancient Three Kingdoms and the Unified Silla era in Korean history. The 14C AMS measurement results presented in this paper on wood charcoal provide precise dates which will be very useful not only to clarify the nature of human activities in this cave but also to provide reference dates when comparing other dating methods.

Lee, J. H.; Choe, K.; Kim, J. C.; Choi, S. H.; Kang, J.; Song, S.; Song, Y. M.; Jang, J. G.

2013-01-01

127

Experiences on radiological monitoring during production of 32P labeled bio-molecules at JONAKI, Hyderabad  

International Nuclear Information System (INIS)

The 32P labeled bio-molecules are very important research tools in the field of modern biology and genetic engineering. JONAKI Laboratory established by the Department of Atomic Energy at Hyderabad handles about 5Ci of 32P in a month for producing the 32P labeled bio-molecules for research. This paper presents experiences on health physics activities which include area/air monitoring, waste management and environmental monitoring

2001-01-01

128

'In vitro' determination of the rate of 32P uptake by erythrocytes  

International Nuclear Information System (INIS)

An ''in vitro'' methodology based on 32p uptake by erythrocytes was established as a potencial method for a phosphorus sub-clinical deficiency diagnosis in ruminant. Blood samples stored up to 48 hours were incubated with 32p at different periods and temperatures. There was no effect of storage time and the greatest 32p uptake values were obtained with incubation over 2 hours at 38 to 500C. (author)

1988-01-01

129

Radiolabelling of humic substances with 14C by azo coupling [14C]phenyldiazonium ions  

International Nuclear Information System (INIS)

For the first time, natural and synthetic humic substances were radiolabelled by azo coupling [U-14C]phenyldiazonium ions onto the aromatic fragments of their macromolecules under mild reaction conditions. The radiolabelling procedure was optimized with respect to pH, reaction temperature and the molar ratio of the humic substance to the labelling compound. The labelled humic substances were purified by precipitation or ultrafiltration. The chemical yields were in the range between 23% and 95%, and the specific radioactivities varied between 68 and 206 MBq 14C per gram of the humic substance, depending on the origin of the humic substance and the purification method. With the 14C-labelled humic compounds thus obtained, we were able to detect humic substances at concentrations as low as 5 ?g/L. These radiolabelled compounds can be used in long-term studies because, according to size exclusion chromatography data, there are no signs of their decomposition even after 5 months of storage

2007-01-01

130

The 32P test and other methods in the diagnosis of intraocular tumours  

International Nuclear Information System (INIS)

[en] Why the 32P test was reintroduced into The Netherlands for the diagnosis of intraocular tumours and how the present study was started is explained. The physical features of 32P and the biochemical behaviour of radioactive phosphate in healthy and neoplastic tissue are described. The complications, which can be encountered after the administration of 32P, are mentioned. A review of the 32P test in ophthalmology is given, with special attention to the methods used and the development of instruments for the measurements. The way in which the 32P test was performed in this study is described. The 32P test results obtained in the patients of this study are described. A good diagnostic accuracy of the 32P test was present in the 192 lesions located in the posterior segment, with a correct result obtained in 186 patients. Only three malignant and three benign lesions showed an incorrect test result. The results obtained with scintigraphy, fluorescein angiography, perimetry, transillumination, and ultrasonography are given and compared with data in the literature. The value of fluorescein angiography, perimetry, and the 32P test for the diagnosis of various lesions, with relevance to the differential diagnosis of a choroidal tumour, is described. The 32P tests performed in 12 patients with an extraocular lesion are mentioned. (Auth.)

1978-01-01

131

Determination of phagocytosis of 32P-labeled Staphylococcus aureus by bovine polymorphonuclear leukocytes  

International Nuclear Information System (INIS)

A procedure for the measurement of phagocytosis by bovine polymorphonuclear leukocytes (PMN) of 32P-labeled Staphylococcus aureus was modified so that a larger number of samples could be compared in a single run, and smaller volumes of sample, PMN, and 32P-labeled S aureus could be used. Results were highly reproducible, with a coefficient of variation between duplicate determinations of less than or equal to 2%. Lysostaphin was prepared from the supernatant of S staphylolyticus and was compared with a commercially available preparation. Effects of lysostaphin on PMN and influence of incubation media on release of 32P from 32P-labeled S aureus by lysostaphin were examined.

1984-01-01

132

A simplified method for the preparation of ?-32P-labeled deoxyribonucleoside triphosphates  

International Nuclear Information System (INIS)

[en] A method for preparing ?-32P-labeled deoxyribonucleoside triphosphates ([?-32P]dNTP) is described. By comparing the labeling procedure to that of Walseth et al. 1,2 the reaction of Nuclease P1 is saved in our experiments and only three steps are needed for a routine preparation, without decreasing the yield of [?-32P]dNTP which is over 92% based on 32Pi. In this paper [?-32P]dATP is taken as an example to describe the labeling procedure, the suitability of our method has been discussed. (author)

1997-01-01

133

Upward and lateral translocation of 32P supplied to roots of apple and citrus trees  

International Nuclear Information System (INIS)

[en] 32P was supplied to the roots of stem-ringed 1-year-old apple trees and 2-year old citrus trees on which bark segments were isolated above and below the ring. 32P was translocated to shoots and leaves although considerable translocation occurred especially in wood and bark tissues. The accumulation of 32P in isolated bark segments indicated that the occurrence of these materials in this tissue was via radial translocation from xylem tissue, and that the main upward translocation pathway of 32P supplied to roots is through the xylem. (author)

1976-01-01

134

Synthesis of N-methyl-14C benzyl amine and N-methyl-14C benzyl nitrosamine  

International Nuclear Information System (INIS)

N-methyl-14C benzyl amine was prepared from benzyl amine through a sequence of trifluoroacylation, ionization, 14C-methylation and hydrolysis. N-methyl-14C benzyl nitrosamine was obtained by treating aqueous 14C-dialkylamine with NaNO2 and H2SO4. The radiochemical purity checked by radio-HPLC and specific activity of the product was 99% and 9.2 x 1011 Bq/mol respectively.

1995-01-01

135

Migration behavior of carbonate-14C in tuffaceous soil  

International Nuclear Information System (INIS)

A simple closed column system was developed to study migration of bicarbonate and carbonate 14C in a soil layer. Experimental data for glass beads having less 14C adsorption was used to test the system in keeping a mass balance of 14C. Migration behavior of bicarbonate and carbonate 14C in a tuffaceous sandy soil was investigated by this column system in the pH range from 8 to 12. The 14C was delayed to tritiated water, and the retardation became stronger in order, pH 12

1995-01-01

136

Environmental 14C activity: the atmosphere and the biosphere  

International Nuclear Information System (INIS)

The natural equilibrium of 14C activity in atmospheric CO2 and in the biosphere has been disturbed globally by various anthropogenic activities. Intensive atmospheric thermonuclear bomb tests in the mid 20th century doubled the natural 14C activity in the atmosphere. Since the bomb test ban, this activity has constantly been decreasing and has almost reached its natural level. This article presents the results of 14C activity monitoring in atmospheric CO2, tree rings and biological samples at several locations in Croatia and Slovenia. Global changes in 14C activity have been observed at clean-airsites. In urban areas, lower atmospheric 14C activity is owed to intense fossil fuel combustion, especially in the winter. Higher 14C activity is observed in near the nuclear power plant during and immediately after refuelling outage. If refuelling is performed in the spring, when plants start to vegetate, increased 14C activity is also reflected on them. (authors)

2010-01-01

137

Preparation and investigation of [14C]padimate-O and [14C]N-nitroso-N-nor-padimate-O  

International Nuclear Information System (INIS)

Carbonation of the aryl lithium obtained from p-N,N(dimethylamino)bromo-benzene with carbon-14 labeled carbon dioxide, followed by esterification gave [CO2-14C]p-N,N(dimethylamino)benzoate 2-ethylhexyl ester (Padimate-O). This material underwent slow decomposition to [14C]N-nor-Padimate-O and [14C]N-formyl-N-nor-Padimate-O. Nitrosation of [14C]Padimate-O with sodium nitrite afforded [14C]N-nitroso-N-nor-Padimate-O. (author).

1995-01-01

138

Elastic and inelastic scattering of 14C + 18O versus 12, 13C + 18O and 14C + 16O  

International Nuclear Information System (INIS)

The angular distributions of the 14C + 18O elastic and inelastic scattering at the energy Elab(18O) = 105 MeV were measured firstly. The data were analyzed within the optical model and the coupled-reaction-channels method. The parameters of the 14C + 18O optical potential were deduced. The contributions of one- and two-step transfers of nucleons and clusters in the 14C + 18O elastic and inelastic scattering were calculated. The isotopic differences between the 14C + 18O and 12, 13, 14C + 18O potentials were studied. (orig.)

2011-01-01

139

Radiophosphorus (32P) treatment of bone marrow disorders in dogs: 11 cases (1970-1987)  

International Nuclear Information System (INIS)

[en] Between March 1970 and February 1987, radiophosphorus (32P) was used to treat bone marrow disorders in 6 dogs; 4 had polycythemia vera and 2 had essential thrombocythemia. Activities of 32P given initially ranged from 2.4 to 3.3 mCi/m2. Four dogs responded well to 32P treatment, with gradual resolution of high RBC or platelet counts. Two of these dogs died of intercurrent disease unrelated to their bone marrow disorder, before blood counts could be stabilized. Two dogs did not respond to the initial 32P treatment nor to additional treatments with 32P, and had clinical signs and blood counts stabilized by use of phlebotomy or chemotherapeutic agents. We reviewed and analyzed 5 other cases of bone marrow disorders in dogs treated with 32P and included the findings from their records with the records of our 6 dogs in this retrospective analysis. Of the 8 dogs with polycythemia vera treated with 32P, 5 were given a single treatment that controlled clinical signs and blood counts for the remainder of the follow-up period. Of the 3 dogs treated for thrombocytosis with 32P, 2 had blood counts that responded to a single treatment

1989-01-01

140

Root Activity Studies of Cashew Plants (Anacardium Occidentale L.) Using exp 32 P Radioisotope.  

Science.gov (United States)

In this preliminary study, we are looking at levels exp 32 P and depths of soil injections on uptake by plants using the injection techniques in root activity studies. exp 32 P activities can be detected in the leaves and flower parts of plants two weeks ...

H. Aminuddin Buggenhout

1981-01-01

 
 
 
 
141

Radiophosphorus (/sup 32/P) treatment of bone marrow disorders in dogs: 11 cases (1970-1987)  

Energy Technology Data Exchange (ETDEWEB)

Between March 1970 and February 1987, radiophosphorus (/sup 32/P) was used to treat bone marrow disorders in 6 dogs; 4 had polycythemia vera and 2 had essential thrombocythemia. Activities of /sup 32/P given initially ranged from 2.4 to 3.3 mCi/m2. Four dogs responded well to /sup 32/P treatment, with gradual resolution of high RBC or platelet counts. Two of these dogs died of intercurrent disease unrelated to their bone marrow disorder, before blood counts could be stabilized. Two dogs did not respond to the initial /sup 32/P treatment nor to additional treatments with /sup 32/P, and had clinical signs and blood counts stabilized by use of phlebotomy or chemotherapeutic agents. We reviewed and analyzed 5 other cases of bone marrow disorders in dogs treated with /sup 32/P and included the findings from their records with the records of our 6 dogs in this retrospective analysis. Of the 8 dogs with polycythemia vera treated with /sup 32/P, 5 were given a single treatment that controlled clinical signs and blood counts for the remainder of the follow-up period. Of the 3 dogs treated for thrombocytosis with /sup 32/P, 2 had blood counts that responded to a single treatment.

Smith, M.; Turrel, J.M.

1989-01-01

142

Clinical observation of 5686 cases of infant hemangioma treated by 32P  

International Nuclear Information System (INIS)

Objective: To explore the therapeutic method of treating infant hemangioma by radioisotopes 32P. Methods: Simple hemangiomata were treated by 32P dermal application method. The authors prepared 32P at the activity of 165.98 kBq/cm2/h, diluted it with 2 ml water, and then dropped it on filter paper. The authors applied the paper to the impaired site according to the shape and the size of hemangioma. The impaired site received 32P radiation at the dosage of 9.41 Gy/cm2 each time. The treatment was repeated once every month. Cavernous hemangiomata were treated by local injections of colloid 32P at the dosage of 370 kBq/cm3 once every 2-3 months. Mixed hemangiomata received combined treatment mentioned above. Results: 3383 cases (99.0%) of simple hemangioma were cured by 32P dermal application method. 1241 case (98.7%) of cavernous hemangioma were cured. 397 cases (83.0%) of mixed hemangioma were cured. 534 cases (98.1%) of naevus flammeus were improved by 32P application method. The total effective rate was 99.8%. The cure rate was 88.3%. Conclusion: Treating hemangioma with 32P is satisfactory for its high efficiency and low toxicity. It may be advisable for infant skin disease

2002-01-01

143

Fate of 14C-labelled compounds in marine environment  

International Nuclear Information System (INIS)

Model ecosystems have played an important role in predicting environmental behavior of agrochemicals. The microcosms used in these studies generally include soil units containing usual biotic components common for that ecosystem. In present studies, scope of two such ecosystems has been extended to study the fate of 14C-labelled pesticides in marine environment. 14C-labelled pesticides used in these studies were chlorpyrifos, DDT and HCH. Two systems were developed in laboratory simulating marine environment to study the fate of these pesticides. The first system was developed in an all glass aquarium tank with marine sediments, seawater, clams and algae and is referred to as marine ecosystem. The second system was developed to permit the total 14C-mass balance studies. It contained marine sediments under moist (60% water holding capacity) or flooded conditions and it is referred to as continuous flow system. Fate of 14C-DDT was studied in marine ecosystem while degradation of 14C-chlorpyrifos and 14C-HCH was studied in continuous flow system. 14C-DDT did not bioaccumulate in clams while at the end of 60 days 50% of the applied 14C-activity was present in sediment fraction of marine ecosystem. 14C-HCH degradation showed about 22-26% mineralization while 45-55% of the applied activity was recovered as organic volatiles. No significant bound residues were formed. 14C-chorpyrifos underwent considerable degradation in marine environment. TCP was the major degradation product. (author)

1999-01-01

144

Method of preparing orotic acid labelled with radioisotope 14C  

International Nuclear Information System (INIS)

[en] The method is presented of universally labelled (U-14C)-orotic acid of a mole activity higher than 250 mCi/mMole. The Claisen condensation with (1,2-14C)-oxalic acid ethylester and (1,2-14C)-acetic acid ethylester having mole activities higher than 50 mCi/milliatom 14C results in the sodium salt of (U-14C)-oxalacetic acid ethylester. When reacted with S-methyl-(14C)-isothiouronium iodide (having an activity higher than 50 mCi/mMole), the substance yields S-methyl-(U-14C)-2-thioorotic acid ethylester whose hydrolysis in an acid medium results in (U-14C)-orotic acid. The (U-14C)-orotic acid can easily be separated from the reaction mixture in the form of precipitate which chemically and radiochemically is almost pure. When an inactive oxalic acid ethylester is used for synthesis, (2,4,5-14C)-orotic acid of a mole activity higher than 150 mCi/mMole can be prepared using the procedure described. (B.S.)

1981-01-01

145

Effect of iodide on glucose oxidation and /sup 32/P incorporation into phospholipids stimulated by different agents in dog thyroid slices  

Energy Technology Data Exchange (ETDEWEB)

Since iodide (I-) inhibits TSH stimulation of cAMP formation, which mediates most of the effects of the hormone, it has been assumed that this accounts for the inhibitory action of iodide on the thyroid. However, TSH stimulation of 32P incorporation into phospholipids and stimulation of thyroid metabolism by other agonists, such as carbachol, phorbol esters, and ionophore A23187, is not cAMP mediated. The present studies examined the effect of iodide on stimulation of glucose oxidation and 32P incorporation into phospholipids by TSH and other agonists to determine if the inhibition of cAMP formation was responsible for the action of iodide. Preincubation of dog thyroid slices for 1 h with iodide (10(-4) M) inhibited TSH-, (Bu)2cAMP-, carbachol-, methylene blue-, 12-O-tetradecanoyl phorbol-13-acetate-, ionophore A23187-, prostaglandin E1-, and cholera toxin-stimulated glucose oxidation. I- also inhibited the stimulation by TSH, 12-O-tetradecanoyl phorbol-13-acetate, carbachol, and ionophore A23187 of 32P incorporation into phospholipids. The inhibition was similar whether iodide was added 2 h before or simultaneously with the agonist. I- itself sometimes stimulated basal glucose oxidation, but had no effect on basal 32P incorporation into phospholipids. The effects of iodide on basal and agonist-stimulated thyroid metabolism were blocked by methimazole (10(-3) M). When dog thyroid slices were preloaded with 32PO4 or (1-14C)glucose, the iodide inhibition of agonist stimulation disappeared, suggesting that the effect of iodide involves the transport process. In conclusion, I- inhibited stimulation of glucose oxidation and 32P incorporation into phospholipids by all agonists, indicating that the effect is independent of the cAMP system and that iodide autoregulation does not only involve this system. Oxidation and organification of iodide are necessary for the inhibition.

Tseng, F.Y.; Rani, C.S.; Field, J.B.

1989-03-01

146

The Protective Effect of Amifostine Against Radioactive Phosphorous (32P) in Mice Peripheral Blood Cells  

Directory of Open Access Journals (Sweden)

Full Text Available The use of radioprotectors such as WR-2721 in radioactive phosphorous is interested because radiation delivered to tumor organ therapy can induce side effects in the bone marrow and most normal organs. To evaluate the potential of WR-2721, we treated mice with this drug (400 mg kg-1) prior administration of 32P at dose 4 ?Ci g-1 intraperitoneally. We determined hematological parameters in treated mice with 32P or WR-2721+32P at twenty days after treatment. Administration of radioprotector failed to enhance RBC, WBC, PLT, Hb and Hct from 32P. In contrast, this radioprotector provided significant protection from total-body gamma irradiation with a DRF 1.91. These results suggest that the potential role of WR-2721 in a 32P therapy is limited because of the difficulty in achieving continuous protection with a single dose of this drug due to its short half-life.

Mohsen Froughizadeh; Seyed Jalal Hosseinimehr

2006-01-01

147

Synthesis of sup 14 C labelled acrylic derivatives: diethyl (3- sup 14 C) methylidenemalonate and isobutyl (3- sup 14 C) cyanoacrylate  

Energy Technology Data Exchange (ETDEWEB)

Isobutyl (3-{sup 14}C) cyanoacrylate and diethyl (3-{sup 14}C) methylidenemalonate were synthesized by the intermediate of their protective Diels-Alder adduct with anthracene. These adducts were obtained in a one-pot procedure by Knoevenagel condensation of ({sup 14}C) paraformaldehyde with isobutyl cyanoacetate and diethyl malonate respectively in the presence of a basic catalyst and anthracene. The adducts are stable crystalline compounds easily purified by recrystallization. The olefinic target compounds were obtained in high chemical and radiochemical purity (>99%) by thermolysis at 220{sup 0}C in mineral oil in the presence of maleic anhydride. (author).

De Keyser, J.-L.; De Cock, C.J.C.; Poupaert, J.H.; Dumont, Pierre (Louvain Univ., Brussels (Belgium). Dept. of Medicinal Chemistry)

1989-08-01

148

A facile preparation of ammonium [14C]thiocyanate  

International Nuclear Information System (INIS)

An attractive and simple method has been developed for the preparation of ammonium [14C]thiocyanate from [14C]thiourea which eliminates the necessity of handling highly hazardous potassium [14C] cyanide. [14C]thiourea was isomerized to ammonium [14C]thiocyanate by heating the aqueous solution of thiourea (12%) in a sealed tube at 160 deg C for 24 hours. The product formed was purified by silica-gel column chromatography. A radiochemical yield of 92.7% was obtained based on [14C]thiourea. The specific activity of the product obtained was 53.3 mCi/mmol (1.97 GBq/mmol) and the radiochemical purity was greater than 99%. This method has not been reported so far for the production of this labeled compound. (author)

2005-01-01

149

Transfer of 14C to prenatal and neonatal rats from their mothers exposed to 14C compounds by ingestion  

International Nuclear Information System (INIS)

The transfer of 14C through placenta or milk was investigated and the radiation dose to fetal and newborn rats was estimated. Female rats at gestational stages or after delivery were exposed to 14C in the form of sodium bicarbonate, thymidine and lysine by a single ingestion. Radioactivity in maternal tissues and conceptuses (placenta, fetal membrane and fetus) and in the newborn was determined at various times after ingestion. After exposure to these 14C compounds, there was no significant difference between the 14C concentration in the fetus and that in the maternal tissues, suggesting that the placenta has no effect in preventing or accelerating the placental transfer of 14C. The concentration and content of 14C in the fetus and newborn were, however, dependent on the chemical form of 14C and on the prenatal or neonatal stage at the time of ingestion. The result of the dose estimation showed that 14C-lysine gave significantly higher prenatal and neonatal doses than 14C-sodium bicarbonate or 14C-thymidine. (author)

2003-01-01

150

14C emission fine structure by Radium isotopes  

International Nuclear Information System (INIS)

[en] First works have shown the fine structure in the 14C spectra emitted by 223Ra and have confirmed the similitude between this emission and ? emission. In this thesis, the author has measured the 14C energy spectra emitted by 222Ra even-even nucleus. In all these experiments, the number of detected carbon has been more important that in the experiment of discovery of these radioactivities. The author states precisely the 14C/? branching ratios and the 14C emission decay for the two studied isotopes

1991-01-01

151

Development of 14C Production Process by Utilizing HANARO  

International Nuclear Information System (INIS)

Feasibility study on the production of 14C by using the HANARO has been performed. As an advantageous tracer, 14C has been used in various chemical applications such as in the drug discovery and development, clinical studies, and environmental fields. The advantageous properties of 14C come from its long half-life (5745 years), which makes sure the 14C-labeled compounds can be used for indefinite time in controlled conditions and its emission of only low-energy beta particles, which provides safer and simpler working conditions. 14C as chemical forms such as barium carbonate (Ba14CO3), methane (14CH4), benzene (14CnC6-nH6), etc are produced in few countries, such as United States, Russia, and UK. Approximately 28.6Ci of 14C was imported in 2005 and mostly consumed by the research or educational organizations. Even though 14C has not been produced in the nation, a local company has well established the labeling technologies of 14C, and commercially produces the labeled compounds. Hence, the feasibility of the production of 14C from the HANARO is evaluated experimentally. In this paper, the experimental results will be compared with the results from a numerical analysis, which was reported previously to support the experimental results

2007-01-01

152

[35S]methionine interaction with rat liver tRNA and effect of chemical carcinogens  

International Nuclear Information System (INIS)

The interaction of [35S]methionine with hepatic tRNA in normal, carcinogen-treated, and partially hepatectomized rats was studied. tRNA was preferentially labeled following [35S]methionine (1.6 mCi, 25 mg/kg body wt) administration by intraperitoneal injection. The extent of [35S]methionine-tRNA interaction was impaired by partial hepatectomy and by conditions having a carcinogenic potential

1991-01-01

153

[35S]methionine interaction with rat liver tRNA and effect of chemical carcinogens.  

UK PubMed Central (United Kingdom)

The interaction of [35S]methionine with hepatic tRNA in normal, carcinogen-treated, and partially hepatectomized rats was studied. tRNA was preferentially labeled following [35S]methionine (1.6 mCi, 25 mg/kg body wt) administration by intraperitoneal injection. The extent of [35S]methionine-tRNA interaction was impaired by partial hepatectomy and by conditions having a carcinogenic potential.

Kanduc D; Quagliariello E

1991-07-01

154

(35S)methionine interaction with rat liver tRNA and effect of chemical carcinogens  

Energy Technology Data Exchange (ETDEWEB)

The interaction of (35S)methionine with hepatic tRNA in normal, carcinogen-treated, and partially hepatectomized rats was studied. tRNA was preferentially labeled following (35S)methionine (1.6 mCi, 25 mg/kg body wt) administration by intraperitoneal injection. The extent of (35S)methionine-tRNA interaction was impaired by partial hepatectomy and by conditions having a carcinogenic potential.

Kanduc, D.; Quagliariello, E. (Dipartimento di Biochimica e Biologia Molecolare, Facolta di Scienze, Universita di Bari (Italy))

1991-07-01

155

Separation of carrier free /sup 32/P by teflon-silica gel column  

Energy Technology Data Exchange (ETDEWEB)

Separation of carrier-free /sup 32/P from neutron irradiated sulfur was studied by using teflon or Pyrex as column material and silica gel or soda lime glass as adsorbent. The highest yield of /sup 32/P was obtained from the the teflon column with pretreated silica gel as adsorbent. Comparison of the result with some other works indicated that the separation condition was improved by using teflon column. Column size as well as particle size of adsorbent had little effect on the separation of /sup 32/P. Eluent of low concentration acid or alkali solution always showed better desorption of /sup 32/P than the high concentration one; however, distilled water was more preferable than any of the solution. Sorption and desorption efficiencies of /sup 32/P decreased with the increasing feeding rate of CS/sub 2/ and elution rate of H/sub 2/O respectively. Practical examples on the production of carrier-free /sup 32/P in TRR were also presented. Chemical and radiochemical analysis demonstrated that the /sup 32/P product thus prepared reached the USP standard.

Lin, T.K.; Hsieh, B.T.; Tsai, Z.T.; Wu, S.C.

1980-09-01

156

Percutaneous absorption of [14C]DDT and [14C]benzo[a]pyrene from soil.  

UK PubMed Central (United Kingdom)

The objective was to determine percutaneous absorption of DDT and benzo[a]pyrene in vitro and in vivo from soil into and through skin. Soil (Yolo County 65-California-57-8; 26% sand, 26% clay, 48% silt) was passed through 10-, 20-, and 48-mesh sieves. Soil then retained by 80-mesh was mixed with [14C]-labeled chemical at 10 ppm. Acetone solutions at 10 ppm were prepared for comparative analysis. Human cadaver skin was dermatomed to 500 microns and used in glass diffusion cells with human plasma as the receptor fluid (3 ml/hr flow rate) for a 24-hr skin application time. With acetone vehicle, DDT (18.1 +/- 13.4%) readily penetrated into human skin. Significantly less DDT (1.0 +/- 0.7%) penetrated into human skin from soil. DDT would not partition from human skin into human plasma in the receptor phase (less than 0.1%). With acetone vehicle, benzo[a]pyrene (23.7 +/- 9.7%) readily penetrated into human skin. Significantly less benzo[a]pyrene (1.4 +/- 0.9%) penetrated into human skin from soil. Benzo[a]pyrene would not partition from human skin into human plasma in the receptor phase (less than 0.1%). Substantivity (skin retention) was investigated by applying 14C-labeled chemical to human skin in vitro for only 25 min. After soap and water wash, 16.7 +/- 13.2% of DDT applied in acetone remained absorbed to skin. With soil only 0.25 +/- 0.11% of DDT remained absorbed to skin. After soap and water wash 5.1 +/- 2.1% of benzo[a]pyrene applied in acetone remained absorbed to skin. With soil only 0.14 +/- 0.13% of benzo[a]pyrene remained absorbed to skin.(ABSTRACT TRUNCATED AT 250 WORDS)

Wester RC; Maibach HI; Bucks DA; Sedik L; Melendres J; Liao C; DiZio S

1990-10-01

157

Percutaneous absorption of [14C]DDT and [14C]benzo[a]pyrene from soil  

International Nuclear Information System (INIS)

The objective was to determine percutaneous absorption of DDT and benzo[a]pyrene in vitro and in vivo from soil into and through skin. Soil (Yolo County 65-California-57-8; 26% sand, 26% clay, 48% silt) was passed through 10-, 20-, and 48-mesh sieves. Soil then retained by 80-mesh was mixed with [14C]-labeled chemical at 10 ppm. Acetone solutions at 10 ppm were prepared for comparative analysis. Human cadaver skin was dermatomed to 500 microns and used in glass diffusion cells with human plasma as the receptor fluid (3 ml/hr flow rate) for a 24-hr skin application time. With acetone vehicle, DDT (18.1 +/- 13.4%) readily penetrated into human skin. Significantly less DDT (1.0 +/- 0.7%) penetrated into human skin from soil. DDT would not partition from human skin into human plasma in the receptor phase (less than 0.1%). With acetone vehicle, benzo[a]pyrene (23.7 +/- 9.7%) readily penetrated into human skin. Significantly less benzo[a]pyrene (1.4 +/- 0.9%) penetrated into human skin from soil. Benzo[a]pyrene would not partition from human skin into human plasma in the receptor phase (less than 0.1%). Substantivity (skin retention) was investigated by applying 14C-labeled chemical to human skin in vitro for only 25 min. After soap and water wash, 16.7 +/- 13.2% of DDT applied in acetone remained absorbed to skin. With soil only 0.25 +/- 0.11% of DDT remained absorbed to skin. After soap and water wash 5.1 +/- 2.1% of benzo[a]pyrene applied in acetone remained absorbed to skin. With soil only 0.14 +/- 0.13% of benzo[a]pyrene remained absorbed to skin

1990-01-01

158

The study of isotopic labelling of dithiocarbamates and dithiophosphates by 35 S  

International Nuclear Information System (INIS)

[en] Studies have been carried out on organic compounds which contain sulphur atoms in their molecule and belong to the group of dithiocarbamates and dithiophosphates, having in view the labelling with 35 S radioisotope. Depending on the working conditions and reaction yields, the labelling with 35 S isotope, was done in two ways: through organic synthesis of reaction intermediate products by using of C 35 S2 or P2 35 S5 or 35 S and through isotopic exchange. The 35 S radioisotope is important due to its applications in the field of organic compound labelling. Among its characteristics: the half time is 87 days, the maximum energy of beta radiation is 0.167 MeV and it is a pure beta transmitter. This work had as purpose: a) synthesizing and labelling of organic compounds from dithiocarbamates and dithiophosphates group by 35 S; b) radiochemical characterization of labelled compounds. The activity measurements were carried out with a 3385 Tricarb-Packard spectrometer, based on liquid scintillators. At the same time, we studied the 35 S labelling technique of these compounds. The organic syntheses used in the process of labelling of these organic compounds, have the advantage to obtain a structure with a definite position of radioactive labelling (of 35 S atom). (authors)

1999-01-01

159

The catabolism of intravenously injected heparan N-[35S]sulphate in the rat  

International Nuclear Information System (INIS)

[en] The metabolic fate of heparan N-[35S]sulphate was studied in rats. Heparan sulphate was obtained from either bovine aorta or lung and labelled with 35S by desulphation and subsequent resulphation in vitro. Experiments in which heparan N-[35S]sulphate was administered intravenously to either free-range or wholly anaesthetized rats with ureter cannulae established that substantial desulphation occurs in vivo, with elimination of inorganic [35S]sulphate in urine. Oligosaccharides labelled with 35S, possible intermediates in heparan sulphate degradation, could not be detected in urine or blood. The general distribution of radioactivity after administration of heparan N-[35S]sulphate, as demonstrated by whole-body radioautography, suggested that desulphation was not restricted to one organ in particular. Support for this view was obtained in experiments in which heparan N-[35S]sulphate was administered to animals after the removal of kidneys, liver, spleen, pancreas or gastrointestinal tract. In all cases inorganic [35S]sulphate was still produced. The ability of rats to desulphate heparan N-[35S]sulphate was progressively impaired by increasing concentrations of heparin administered simultaneously. It was concluded that heparan sulphate is metabolized at a number of sites in the body by a sequence of degradative events leading to the formation of inorganic sulphate. It is also concluded that at least some of these events are common to heparan sulphate and heparin

1977-09-15

160

The transfer of different forms of {sup 35}S to goat milk  

Energy Technology Data Exchange (ETDEWEB)

Sulphur-35 is released during the routine operation of UK gas-cooled reactors. An experiment to determine the rates of transfer of different forms of {sup 35}S to goat milk is described. Lactating goats received {sup 35}S orally as single administrations of sulphate, L-methionine, or grass contaminated either through root uptake of {sup 35}S as sulphate or through aerial deposition of {sup 35}S as carbonyl sulphide onto the grass. Transfer was higher for {sup 35}S administered as methionine compared with {sup 35}S administered as sulphate. Changes in activity concentrations in milk for all sources of {sup 35}S demonstrated two components of loss. The first component had a half-life of circa 1 d for all sources, the second was longer in goats administered carbonyl sulphide (44 d) than in all of the other treatments (circa 10 d). The rate of transfer of {sup 35}S to milk of a further group of goats receiving {sup 35}S-sulphate daily appeared to reach equilibrium within 30 d. Extrapolation of transfer parameters derived to other dairy ruminants is discussed.

Howard, B.J. [Centre for Ecology and Hydrology, Lancaster Environment Centre, Library Avenue, Bailrigg, Lancaster LAI 4AP (United Kingdom)], E-mail: bjho@ceh.ac.uk; Beresford, N.A. [Centre for Ecology and Hydrology, Lancaster Environment Centre, Library Avenue, Bailrigg, Lancaster LAI 4AP (United Kingdom); Mayes, R.W.; Lamb, C.S. [Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH (United Kingdom); Barnett, C.L. [Centre for Ecology and Hydrology, Lancaster Environment Centre, Library Avenue, Bailrigg, Lancaster LAI 4AP (United Kingdom)

2007-11-15

 
 
 
 
161

Enzymatic method for the synthesis of (/sup 14/C)pyridoxal 5-phosphate from (/sup 14/C)pyridoxine  

Energy Technology Data Exchange (ETDEWEB)

A new enzymatic method for the synthesis of (/sup 14/C)pyridoxal 5-phosphate is presented. (/sup 14/C)Pyridoxal 5'-phosphate was synthesized from (/sup 14/C)pyriodoxine through the successive actions of pyridoxal kinase and pyrdoxamine 5'-phosphate oxidase in a reaction mixture containing ATP, (/sup 14/C)pyridoxine, and both enzymes. (/sup 14/C)Pyridoxal 5'-phosphate was isolated by omega-aminohexyl-Sepharose 6B column chromatography. The overall yield of the product was more than 60%, starting from 550 nmol of (/sup 14/C)pyridoxine. The radiochemical purity of the products, as determined by thin-layer and ion-exchange chromatography, was greater than 98%.

Yagi, T.; Takasugi, M.; Yamamoto, S.; Nozaki, M.

1986-10-01

162

Synthesis of [3-14C]-L-tryptophan and 5'-hydroxy-[3-14C]-L-tryptophan  

International Nuclear Information System (INIS)

The synthesis of selectively labeled [3-14C]-L-tryptophan and its derivative 5'-hydroxy-[3-14C]-L-tryptophan using chemical and multienzymatic methods is reported. The key intermediate for this synthesis, 3-14C]-DL-alanine was obtained from 14CH3I as a result of its condensation with N- (diphenylmethylene)glycine tert-butyl ester. Next, the mixture containing [3-14C]-DL-alanine, indole or 5-hydroxyindole has been converted to [3-14C]-L-tryptophan or 5'-hydroxy-[3-14C]-L-tryptophan, respectively, in a one-pot multienzymatic reaction using four enzymes: -amino acid oxidase, catalase, glutamic-pyruvic transaminase and tryptophanase. (author)

2009-01-01

163

Method of preparing D-mannose(U-14C) from glucons(U-14C) separated from natural material  

International Nuclear Information System (INIS)

Glucans(U-14C) separated from green or blue-green algae are hydrolysed using diluted mineral acids in the presence of small amounts of molybdate ions to D-glucose(U-14C) which, at a temperature of 60 to 100 degC epimerizes to D-mannose(U-14C). The epimeric aldoses are separated from the reaction mixture by paper chromatography. (H.S.).

1981-01-01

164

Root-uptake of 14C derived from acetic acid and 14C transfer to rice edible parts  

International Nuclear Information System (INIS)

Three types of culture experiments using paddy rice (Oryza sativa L.) were performed to examine root-uptake of 14C in the form of acetic acid: double pot experiment (hydroponics), wet culture experiment (submerged sand medium), and chamber experiment (hydroponics and submerged sand medium). The 14C radioactivity in the plant, mediums, and atmospheric carbon dioxide (14CO2) in the chamber were determined, and the distribution of 14C in the plant was visualized using autoradiography. In the double pot experiment, the shoot of the plant and the lower root which was soaked in the culture solution had 14C radioactivity, but the upper root which did not have contact with the solution had none. There were also 14C radioactivity in the grains and roots in the wet culture experiment. Results of the chamber experiment showed that 14CO2 gas was released from the culture solution in both types of cultures. Results indicated that the 14C-acetic acid absorbed by rice plant through its root would be very small. Most of the 14C-acetic acid was transformed into gaseous forms either in the culture solution or rhizosphere. A relatively longer time would be needed to assimilate 14C derived from acetic acid to grain parts after it was once absorbed by the shoot through the root. Availability of 14C for the plant in sand culture was considered to be decreased compared with that for the plant in the hydroponics experiment. It was suggested that rice plant absorbed and assimilated 14C through the plant roots not because of uptake of 14C-acetic acid but because of uptake of 14C in gaseous forms such as 14CO2.

2010-01-01

165

Facile conversion of [1-14C]lauronitrile to [1-14C]lauric acid under microwave irradiation  

International Nuclear Information System (INIS)

A highly efficient and an optimized synthesis of [1-14C]lauric acid with high specific activity (50 mCi/mmol) is described. [1-14C]lauric acid was prepared from [1-14C]lauronitrile, in 2 minutes with a mixture of concentrated hydrochloric acid: propionic acid (1: 2 v/v) under microwave irradiation, in quantitative yield. (author)

2006-01-01

166

Study on labelling of rapeseed mustard plants by 32P radiotracer  

International Nuclear Information System (INIS)

Present investigation was done to label rapeseed-mustard plants (Brassica campestris cv. YST-151 and Brassica juncea cv. Varuna) by using 32P radiotracer technique by different methods. Phosphorous being an essential nutrient, plant absorb reasonable amount of 32P thus enabling an easy way of detection. Different methods were used to label the host plant with 32P namely application of radioisotope in soil; injection of radioisotope into plant stem and application of radioisotope in nutrient solution. The best method was found by application of radioisotope in the nutrient solution as it gave highest uptake and required least time out of the 3 given methods for labeling of plants. (author)

2009-01-01

167

14C production and release behaviour in HTR-PM  

International Nuclear Information System (INIS)

14C has a long half-life and can enter the biosphere with the stable isotope. It is concerned in environmental impact assessment of nuclear power plants. Models of 14C production and release from HTR-PM were established, and annual production and release were estimated. The result was compared with CANDU. (authors)

2009-01-01

168

Disposition of 14C tolrestat in laboratory animals and man  

International Nuclear Information System (INIS)

The disposition of the aldose reductase inhibitor tolrestat (T) was determined in the mouse, rat, dog, assemensis monkey, and man. Serum T and radioactivity ratios, and % of dose excreted after p.o. administration of 14C-T at a dose of 10 mk/kg (100 mg to man), are presented. Except for the rat and monkey, 55 to 95% of the urinary 14C was due to T and oxo-tolrestat (oxo-T, N-[[5-(trifluoromethyl)-6-methoxy-1-napthalenyl]oxo-methyl]-N-methylglycine). Oxo-T is formed, in part, non-enzymatically from T; a potential intermediate in this transformation was detected in all the urine samples. In man and monkey, about 15% and 68%, respectively, of the urinary 14C was due to T-glucuronide. In rat urine, >90% of the 14C was due to polar metabolites; in bile, about 66% of the 14C was due to T. The composition of the serum 14C in the mouse and rat was determined. In conclusion, 14C-T was rapidly and well absorbed by all species. Except for the rat and dog, urine was the main excretion route for 14C. Urine14 comprised mainly T, t-glucuronide of oxo-T in all the species except the rat.

1986-01-01

169

?-D-[U-14C]glucose monophosphates  

International Nuclear Information System (INIS)

[en] The standard applies to ?-D-[U-14C]glucose-1-phosphate and ?-D-[U-14C]glucose-6-phosphate. Structural formulas and relative molecular weights are presented valid for the non-active compound, chemical preparation, specifications for molar activity and radiochemical purity, testing, packaging, transport and storage. (J.P.)

1982-01-01

170

Synthesis of specific labelled [methyl-14C]sarin  

International Nuclear Information System (INIS)

[en] The synthesis of [methyl-14C]sarin ([14C]methylphosphono-fluoridic acid 1-methylethyl ester) was accomplished by another approach as for nonlabelled sarin in a tele-conducted reaction vessel. The purity was estimated by IR, GC, and GC-MS and the stability in different media was examined. (author)

1988-01-01

171

Dilute Nuclear States: 12C, 10Be and 14C  

International Nuclear Information System (INIS)

The experimental evidence for dilute ?-particle states in 12C, 10Be and 14C is discussed. The question of the location of the 2+ excitation of the 7.65 MeV 12C state remains unresolved, as does the existence of possible analogue states in 14C.

2008-11-11

172

Radiation dose and biological effects to mouse testis from sodium 32P-phosphate  

International Nuclear Information System (INIS)

[en] Radiation dose to mouse testis was estimated to be about 1.65 rad per ?Ci of intravenously injected 32P. This high dose to the organ was due to the incorporation of this isotope into the macromolecules of the testis. Up to 30% of the total testis activity was in DNA molecules. Biologic effects on mouse testis from 32P were determined by testis weight loss and the decrease in the number of sperm heads in the testis. Number of sperm heads reached a minimum of 1.3% of control 36 days after injection of 3.5 ?Ci/g body weight of 32P. Significant decreases in sperm head counts were observed after as little as 0.2 ?Ci/g body weight of 32P. (author)

1982-01-01

173

Uptake and distribution of 32P in the budded and self-rooted grape varieties  

International Nuclear Information System (INIS)

[en] In the self-rooted and budded varieties of grape (Vitis vinifera L.), the total P and 32P contents were high in 'Anabee-Shahi', but low in in 'Muscat'. The growing shoots contained more P than old stems and roots in all the varieties. In the budded plants, 'Kali Sahebi' scion budded on 'Anab-e-Shani showed the maximum 32P and total P in the shoots, but 'Muscat' scion budded on 'Anab-e-Shahi' accumulated more P in the roots and very low 32P in the growing shoots. Auto-radiographs of shoots also showed that 'Kali Sahebi' budded on 'Anab-e-Shani' rootstock accumulated more 32P in the shoots. (author)

1974-01-01

174

Standardization of 32P/33P and 204Tl by liquid scintillation counting.  

UK PubMed Central (United Kingdom)

Two solutions, one of 32P with a significant contribution of 33P, and another one of 204Tl were standardized by liquid scintillation counting in the frame of two intercomparison exercises organized by the BIPM in 2002. For the 32P solution, the counting efficiencies for 32P and 33P were obtained by the CIEMAT/NIST method. The individual contributions of 32P and 33P were determined by fitting a set of measurements to a function of the half-lives and counting efficiencies of both component nuclides. 204Tl was also standardized by the CIEMAT/NIST method using two counters, three scintillators, HiSafe 3, Insta-Gel Plus and Ultima-Gold, and two different kinds of samples. A total of 480 samples were measured over 45 days with results in full agreement within the uncertainties considered. The efficiency values are also compared to those used in the previous 204Tl intercomparison (1997).

Barquero LR; García-Toraño E; Los Arcos JM

2004-02-01

175

Root activity studies of cashew plants (Anacardium Occidentale L.) using 32P radioisotope  

International Nuclear Information System (INIS)

In this preliminary study, we are looking at levels 32P and depths of soil injections on uptake by plants using the injection techniques in root activity studies. 32P activities can be detected in the leaves and flower parts of plants two weeks after injection in the soil. Reasonable count rate can be obtained by using lower level of 32P (1.6 mCi/tree) than recommended (5 mCi/tree). 32P counts were higher in younger than older leaves. Flowers had the least count. Differences in injection depths (5 and 15 cm) could not be detected. Differences in count rate were detected due to position of leaves and flowers (upper and lower canopy) in some of the sampling intervals. (author)

1981-06-11

176

Determination of phagocytosis of /sup 32/P-labeled Staphylococcus aureus by bovine polymorphonuclear leukocytes  

Energy Technology Data Exchange (ETDEWEB)

A procedure for the measurement of phagocytosis by bovine polymorphonuclear leukocytes (PMN) of /sup 32/P-labeled Staphylococcus aureus was modified so that a larger number of samples could be compared in a single run, and smaller volumes of sample, PMN, and /sup 32/P-labeled S aureus could be used. Results were highly reproducible, with a coefficient of variation between duplicate determinations of less than or equal to 2%. Lysostaphin was prepared from the supernatant of S staphylolyticus and was compared with a commercially available preparation. Effects of lysostaphin on PMN and influence of incubation media on release of /sup 32/P from /sup 32/P-labeled S aureus by lysostaphin were examined.

Dulin, A.M.; Paape, M.J.; Weinland, B.T.

1984-04-01

177

PEMANFAATAN RADIOISOTOP 32P UNTUK PENANDAAN (LABELLED COMPOUND) PADA NYAMUK Aedes aegypti  

Directory of Open Access Journals (Sweden)

Full Text Available An Ae. aegypti mosquitoes labelling with Radioisotop 32P was performed at various dose application. The research conducted by Insitute of Vector and Reservoir Control Research and Development, Salatiga in collaboration with The National of Atomic Agency that aimed to know the effective dose and radioactivity disposal of the Radioisotop 32 P. The research used several doses: 0,3 µCi (micro currie); 0,5 µCi; and 0,7 µCi of each 25 gr larvaefood for 50 larvae with dry and wet radiation then observed the effect of radiation against larvae stadium and mosquitoes. The result shows that at 0,5 pCi isotop 32P dose application, Ae. aegypti mosquitoe can survive with 333,3 cps (currie per second) residual radioactivity and detected in 75 cm distance. The Radioisotop 32P can be used as Ae. aegypti mosquitoes labelling/marking.

Akhid Darwin; Lulus S.; Ali Rahayu

2012-01-01

178

32P and acute leukemia: development of leukemia in a patient with hemoglobin Yakima  

International Nuclear Information System (INIS)

[en] In 1954 a then 31-yr-old male was found to have erythrocytosis. Over the ensuing decade he received 72 mCi 32P. In 1964 his daughters were found to have erythrocytosis. Further investigation led to the discovery of hemoglobin Yakima, a variant with high oxygen affinity. He received no further therapy and was well until 1975, when he developed the preleukemic syndrome. Within 12 mo he developed acute nonlymphocytic leukemia accompanied by fetal erythropoiesis. Because the initial discovery of this type of hemoglobinopathy came 27 yr after the introduction of 32P for use in the treatment of polycythemia vera, and because there are now known to be more than 39 different high-oxygen-affinity hemoglobins, we anticipate that more patients such as ours have been exposed to 32P. The exposed population should be closely followed, since this will likely permit assessment of the risk of 32P-induced leukemia in a nonneoplastic condition

1978-01-01

179

Myo-inositol-14C, phytic acid-14C and ferric phytate-14C metabolism through microbian action in an andosol soil  

International Nuclear Information System (INIS)

The myo-inositol-14C, phytic acid-14C and ferric phytate-14C compounds were incubated in an andosol soil at 70% of the field capacity and at 36.5 deg C during twelve days. These compounds suffered a microbian oxidation at 14CO2 of 61.0, 1.9 and 0% respectively. The fixation of the phytic acid-14C was observed through the fast decrease in the metabolism, due to the formation of complexes with the Fe and Al (phytates). The myo-inositol-14C metabolism was reduced by a factor of nine at the second incubation day. The following mechanisms were observed in the myo-inositol metabolism: (i) adsorption of the inositol by the soil minerals, (ii) adsorption by humic acids, (iii) myo-inositol phosphorylation and (iv) epimerization of myo-inositol to chiro-inositol. It was found that the (i) and (ii) formation depends on the soil microbian activity. The (i), (ii) and (iii) interactions were considered as possible mechanisms for the inhibition of the myo-inositol microbian oxidation. The inhibition of the myo-inositol oxidation through adsorption or phosphorylation is considered as a chemical blockade for the hydroaxial group, avoiding this way a microbian oxidation stereospecific of this hydroxil group. (author)

1977-01-01

180

Preparation of 35S-labeled natural thiophene derivatives by biosynthesis in Tagetes sp  

International Nuclear Information System (INIS)

Some 35S labeled thiophene derivatives have been prepared by biosynthesis in in vitro cultures of Tagetes sp. A different sources of isotopic sulfur were tested and the best results were achieved with [35S] sodium sulfate. Elemental sulfur was not incorporated. Radiochemical yields were around 1%. (author). 12 refs., 4 figs., 3 tabs.

1996-01-01

 
 
 
 
181

Cerenkov counting of 32P in plant samples using Cr2O3 as a quencher  

International Nuclear Information System (INIS)

32P radionuclide is widely used as radiotracer for phosphorous in plant and animal research. Cerenkov counting of 32P using LSC is found much more efficient and convenient. Cr2O3 aqueous solution was found good quencher for the developing quench calibration curve using SCR method. The optimal, volume of sample solution was found for 10-12 ml and concentration of 4-methylumbelliferone at 0.050-0.100 g L-1. (author)

2001-01-01

182

Determination of a calibration factor for the nondestructive assay of Guidant {sup 32}P brachytherapy sources  

Energy Technology Data Exchange (ETDEWEB)

A calibration factor ('dial setting') for the nondestructive assay of Guidant TiNi-encapsulated {sup 32}P intravascular brachytherapy wire sources has been determined for measurements with the Capintec CRC-12 (sic. 'dose calibrator') ionization chamber. The calibration factor was derived from ionization current measurements with the CRC-12 followed by very quantitative, destructive assays of the {sup 32}P content in two sources.

Colle, R.; Zimmerman, B.E.; Soares, C.G.; Coursey, B.M

1999-05-01

183

Radioactive 32P incorporation in liver of mice fed with Aspergillus terreus contaminated feed  

International Nuclear Information System (INIS)

The distribution of 32P during toxicosis due to Aspergillus terreus, a common food contaminant, reported to procedure the toxin terreic acid, in addition to few others, was studied in mice. Radioactive 32P was injected intraperitoneally to the control mice and the experimental ones, which were fed with Aspergillus terreus contaminated feed, as well as the toxin terreic acid. After 24 hrs, both control and experimental animals were sacrificed. 32P incorporation in various fractions of liver were studied. 0.5 cm3 of each fractions was spread on a planchette and dried at 60 deg C. 32P activity was measured using a thin end window Geiger Mueller tube connected to Panax-type 100 C counter. No corrections were necessary for self absorption. In mice, fed with the contaminated feed, more 32P got incorporated in the nucleic acid fraction than seen in protein, barium soluble and barium insoluble fractions, whereas 32P incorporation in lipid fraction was lower. (T.G.)

1978-01-01

184

32P-postlabeling analysis of non-radioactive aromatic carcinogen-DNA adducts  

International Nuclear Information System (INIS)

A newly developed enzymatic 32P-postlabeling method was applied to the analysis of DNA's containing non-radioactive arylamine, arylamide, and polycyclic aromatic hydrocarbon adducts. DNA reacted in vitro with N-hydroxy-2-amino-fluorene, N-acetoxy-2-acetylaminofluorene, and 7#betta#,8?-dihydroxy-9?, 10?-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene, respectively, as well as DNA preparations from the livers of rats treated with N-hydroxy-2-acetylaminofluorene and benzo[a]pyrene, respectively, were enzymatically digested to deoxyribonucleoside 3'-monophosphates, which were then converted to [5'-32P]deoxyribonucleoside 3',5'-bisphosphates by T4 polynucleotide kinase-catalyzed [32P]phosphate transfer from [#betta#-32P]ATP. The 32P-labeled nucleotides were resolved by anion-exchange t.l.c. on polyethyleneimine-cellulose and detected by autoradiography. Aromatic adduct nucleotides were found to be retained at the origin in aqueous electrolyte solutions but to migrate as distinct spots in solvents containing 7-8.5 M urea. Advantage was taken of this observation to remove 32P-labeled normal DNA nucleotides from adduct nucleotides. This purification enabled the detection of a single adduct in 107-108 normal nucleotides. The method appears applicable to the ultrasensitive detection of a large number of carcinogen-DNA adducts of diverse structure without requiring radioactive carcinogens or specific antibodies

1982-01-01

185

32P-postlabeling analysis of non-radioactive aromatic carcinogen-DNA adducts  

International Nuclear Information System (INIS)

A newly developed enzymatic 32P-postlabeling method was applied to the analysis of DNA's containing non-radioactive arylamine, arylamide, and polycyclic aromatic hydrocarbon adducts. DNA reacted in vitro with N-hydroxy-2-amino-fluorene, N-acetoxy-2-acetylaminofluorene, and 7?,8?-dihydroxy-9?, 10?-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene, respectively, as well as DNA preparations from the livers of rats treated with N-hydroxy-2-acetylaminofluorene and benzo[a]pyrene, respectively, were enzymatically digested to deoxyribonucleoside 3'-monophosphates, which were then converted to [5'-32P]deoxyribonucleoside 3',5'-bisphosphates by T4 polynucleotide kinase-catalyzed [32P]phosphate transfer from [?-32P]ATP. The 32P-labeled nucleotides were resolved by anion-exchange t.l.c. on polyethyleneimine-cellulose and detected by autoradiography. Aromatic adduct nucleotides were found to be retained at the origin in aqueous electrolyte solutions but to migrate as distinct spots in solvents containing 7-8.5 M urea. Advantage was taken of this observation to remove 32P-labeled normal DNA nucleotides from adduct nucleotides. This purification enabled the detection of a single adduct in 107-108 normal nucleotides. The method appears applicable to the ultrasensitive detection of a large number of carcinogen-DNA adducts of diverse structure without requiring radioactive carcinogens or specific antibodies. (author)

1982-01-01

186

Use of (32)P to study dynamics of the mitochondrial phosphoproteome.  

UK PubMed Central (United Kingdom)

Protein phosphorylation is a well-characterized regulatory mechanism in the cytosol, but remains poorly defined in the mitochondrion. In this study, we characterized the use of (32)P-labeling to monitor the turnover of protein phosphorylation in the heart and liver mitochondria matrix. The (32)P labeling technique was compared and contrasted to Phos-tag protein phosphorylation fluorescent stain and 2D isoelectric focusing. Of the 64 proteins identified by MS spectroscopy in the Phos-Tag gels, over 20 proteins were correlated with (32)P labeling. The high sensitivity of (32)P incorporation detected proteins well below the mass spectrometry and even 2D gel protein detection limits. Phosphate-chase experiments revealed both turnover and phosphate associated protein pool size alterations dependent on initial incubation conditions. Extensive weak phosphate/phosphate metabolite interactions were observed using nondisruptive native gels, providing a novel approach to screen for potential allosteric interactions of phosphate metabolites with matrix proteins. We confirmed the phosphate associations in Complexes V and I due to their critical role in oxidative phosphorylation and to validate the 2D methods. These complexes were isolated by immunocapture, after (32)P labeling in the intact mitochondria, and revealed (32)P-incorporation for the alpha, beta, gamma, OSCP, and d subunits in Complex V and the 75, 51, 42, 23, and 13a kDa subunits in Complex I. These results demonstrate that a dynamic and extensive mitochondrial matrix phosphoproteome exists in heart and liver.

Aponte AM; Phillips D; Hopper RK; Johnson DT; Harris RA; Blinova K; Boja ES; French S; Balaban RS

2009-06-01

187

Use of (32)P to study dynamics of the mitochondrial phosphoproteome.  

Science.gov (United States)

Protein phosphorylation is a well-characterized regulatory mechanism in the cytosol, but remains poorly defined in the mitochondrion. In this study, we characterized the use of (32)P-labeling to monitor the turnover of protein phosphorylation in the heart and liver mitochondria matrix. The (32)P labeling technique was compared and contrasted to Phos-tag protein phosphorylation fluorescent stain and 2D isoelectric focusing. Of the 64 proteins identified by MS spectroscopy in the Phos-Tag gels, over 20 proteins were correlated with (32)P labeling. The high sensitivity of (32)P incorporation detected proteins well below the mass spectrometry and even 2D gel protein detection limits. Phosphate-chase experiments revealed both turnover and phosphate associated protein pool size alterations dependent on initial incubation conditions. Extensive weak phosphate/phosphate metabolite interactions were observed using nondisruptive native gels, providing a novel approach to screen for potential allosteric interactions of phosphate metabolites with matrix proteins. We confirmed the phosphate associations in Complexes V and I due to their critical role in oxidative phosphorylation and to validate the 2D methods. These complexes were isolated by immunocapture, after (32)P labeling in the intact mitochondria, and revealed (32)P-incorporation for the alpha, beta, gamma, OSCP, and d subunits in Complex V and the 75, 51, 42, 23, and 13a kDa subunits in Complex I. These results demonstrate that a dynamic and extensive mitochondrial matrix phosphoproteome exists in heart and liver. PMID:19351177

Aponte, Angel M; Phillips, Darci; Hopper, Rachel K; Johnson, D Thor; Harris, Robert A; Blinova, Ksenia; Boja, Emily S; French, Stephanie; Balaban, Robert S

2009-06-01

188

Helicobacter pylori infection studies using 14C UBT method  

International Nuclear Information System (INIS)

Carbon 14 (14C) is a naturally occurring radioactive isotope of carbon. The half-life of 14C is about 5,730 years, and it decays by emitting a low energy beta (â) particle of mean energy 49 KeV. The biological half life of 14C is 10 to 12 days. 14C is present in the environment and in all organic materials and behaves in the environment in the same manner as other carbon isotopes. In medicine, 14C can be injected to study abnormalities of metabolism that underlie diabetes, gout, anaemia and acromegaly (adult 'gigantism'), and for diagnosis of Helicobacterpylori (H pylori), The discovery H Pylori by Warren and Marshall in 1982 changed the approach to treat peptic ulcer disease (PUD), Since then H pylori has been the focus of clinical research and debate. The causal relationship between H pylori infection and chronic gastritis is well established, H pylori infection is one of the most common human infections worldwide. This organism has been shown to infect over half of the world's population. By the application of radiation much progress has been made worldwide in the field of medicine. This article presents the application of 14C Urea Breath Test (14C UBT) for the diagnosis of the H pylori bacteria which present in the stomach and duodenum, 14C UBT relies on the urease activity of H pylori to detect the presence of active infection. Board of Radiation and Isotope Technology (BRIT), Mumbai has developed 14C UBT method and already in regular practice in many hospitals across the country. Orally administered 14C urea will be hydrolyzed into ammonia (NH4) and 14C labelled carbon dioxide (14CO2). The presence of a significant amount of (14CO2) in the exhaled breath indicates active H pylori infection. 14C UBT is relatively inexpensive, is easy to perform, and does not require endoscopy. 14C UBT has proved to be one of the most accurate methods for assessing H pylori status. The ionizing radiation dose involved in this test is extremely low, much lower than the radiation dose due to natural sources. Nearly 100 samples collected from both symptomatic and non-symptomatic persons of Dakshina Kannada District, Karnataka were analysed and the results with discussion are presented in this paper. (author)

2012-01-01

189

Chloroquine-Resistant Plasmodium falciparum: Difference in the Handling of 14C-Amodiaquin and 14C-Chloroquine  

Digital Repository Infrastructure Vision for European Research (DRIVER)

14C-amodiaquin and 14C-chloroquine were used to study drug binding by preparations of owl monkey erythrocytes infected either with a chloroquine-susceptible (CS) or with a chloroquine-resistant (CR) strain of P. falciparum. Both of these drugs are derivatives of 4-aminoquinoline, but they differ in ...

Fitch, Coy D.

190

Synthesis of 2-acetoxy 4-trifluoromethyl benzoic acid [ring U-14C] or '[ring U-14C] triflusal'  

International Nuclear Information System (INIS)

(Ring-U-14C) Aniline (1) was converted into (ring U-14C) idobenzene (2) through the (ring U-14C) phenyldiazonium chloride. Trifluoromethylation of (2) with iodotrifluoromethane in presence of copper gave (ring U-14C) trifluoromethylbenzene (3) with a 64% yield. Nitration of (3) with sodium nitrate + trifluoromethane sulfonic acid in dichloromethane gave a 86% yield of (ring U-14C) 3-nitro trifluoromethyl benzene (4) which was reduced by iron and HCl into the corresponding amine (5). The latter was transformed into the phenol (6) with a 85% yield by action of Cu (NO3)2 and CuO on (ring U-14C) 3-trifluoromethyl phenyldiazonium sulfate. Kolbe reaction of phenol (6) with carbon dioxide and K2CO3 gave (7) with a 59% yield. Acetylation of (7) gave (ring U-14C) Triflusal (8) with a quantitative yield and a specific activity of 14 mCi/mMole radiochemical purity 98.5%. The overall yield from 14C barium carbonate was 10%. (author).

1982-01-01

191

Biosynthesis of [14C]zearalenone from [1-14C]acetate by Fusarium roseum 'Gibbosum'.  

UK PubMed Central (United Kingdom)

Addition of [1-14C]acetate or [1,2-14C]acetate to actively growing cultures of Fusarium roseum 'Gibbosum' on rice yielded zearalenone with a specific activity ranging between 1.63 and 46.5 microCi/mmol.

Hagler WM; Mirocha CJ

1980-03-01

192

Biosynthesis of [14C]zearalenone from [1-14C]acetate by Fusarium roseum 'Gibbosum'.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Addition of [1-14C]acetate or [1,2-14C]acetate to actively growing cultures of Fusarium roseum 'Gibbosum' on rice yielded zearalenone with a specific activity ranging between 1.63 and 46.5 microCi/mmol.

Hagler, W M; Mirocha, C J

193

Aspects of the metabolism of U-14C arginine, U-14C histidine and U-14C lysine by adult female Glossina morsitans during pregnancy  

International Nuclear Information System (INIS)

[en] U-14C arginine, U-14C histidine or U-14C lysine was injected into haemolymph of pregnant female Glossina morsitans. Radioactivity was detected in the post-parturient female and its larval offspring mostly in arginine or histidine, but in the case of lysine injection radioactivity was detected in the two life stages in lysine lipids and a range of nonessential amino acids. The pattern of radioactivity in the developing oocyte and larva was related to growth characteristics of these young stages. Whereas arginine and histidine were mostly excreted unchanged, lysine derived radioactivity was detected in the excreted uric acid and expired carbon dioxide; radioactivity in such products was greater in early than late pregnancy. (author)

1977-01-01

194

Secretin enhances [14C]erythritol clearance in unanesthetized dogs  

International Nuclear Information System (INIS)

To determine the effect of secretin infusion on clearance of inert markers into bile, unanesthetized dogs fitted with Thomas cannulas received continuous infusions of [14C]erythritol and [3H]inulin throughout study. Taurocholic acid administered sequentially at 9.0, 20.0, and 40.0 mumol/min enhanced [14C]erythritol clearance, and GIH secretin (3 units/min) administered along with TCA (40.0 mumol/min) increased [14C]erythritol clearance from 4.9 +/- 1.2 ml/10 min to 6.8 +/- 1.3 ml/10 min (P less than 0.001), but simultaneously measured [3H]inulin clearance was unaltered. Secretin alone also increased [14C]erythritol clearance but did not alter [3H]inulin clearance. The increase in [14C]erythritol clearance per unit increase in bile flow was less during secretin infusion than TCA. Thus, secretin increases [14C]erythritol transport through restricted channels, probably distal to the canaliculi. [14C]Erythritol may not be an accurate marker for canalicular bile flow in dogs during secretin infusion.

1982-01-01

195

Pulse height spectral analysis of 3H:14C ratios  

International Nuclear Information System (INIS)

Activity ratios of 3H:14C of isotope-labeled substances are employed commonly in biochemical studies of reaction mechanisms and pathways. The liquid scintillation pulse height spectral index (SIS) of a composite sample of 3H and 14C was demonstrated to provide an index to the 3H:14C activity ratio of the sample. Linear and logarithmic relations between the 3H:14C activity ratio and SIS were defined for a given level of quench. When the level of quench is maintained constant, the SIS was demonstrated to be directly correlated to the 3H:14C activity ratios of the sample. A mean recovery of 102.3% was obtained for calculated 3H:14C activity ratios over the range of 0.097:1-9.698:1. The method described provides the 3H:14C activity ratios of samples without the need for determining the separate activities of the individual radionuclides of the dual label. (Author).

1996-01-01

196

Absorption, Translocation and Metabolism of 14C-Labelled Dichlobenil  

International Nuclear Information System (INIS)

[en] Autoradiographs of bean (Phaseolus vulgaris L.) and alligatorweed (Alternanthera philoxeroides (Mart.) Griseb.) indicated that 14C-nitrile-labelled dichlobenil (2,6-dichlorobenzonitrile) was slightly absorbed by the leaf and some translocation occurred following foliar application. Plants with roots submersed in radioactive aqueous solution absorbed and translocated the 14C throughout the plant. An investigation of some of the chemical and physical properties of 14C-nitrile-labelled dichlobenil was conducted. Loss because of volatilization from counting planchets was considerably reduced by application of acrylic plastic immediately after the solution dried. The plastic coating also eliminated contamination of counting chambers and windows. Two higher plants (bean and alligatorweed ) and four fungi (Fusarium sp., Geotrichum sp., Penicillium sp., Trichoderma sp.) were selected for metabolism studies. Dichlobenil- 14C was added to Hoagland and Arnon's nutrient solution containing beans or alligatorweed and to liquid cultures containing the other organisms for 12 to 120 h. Extracts from the plants or fungi were chromatographed on silica gel thin-layers. Autoradiographs of the thin-layer chromatographed aqueous extracts revealed a C-labelled compound of Rf 0.25 that differed from that of dichlobenil, which was 0.6. After esterification of the extracts, a 14C-labelled compound was observed at Rf 0.95 on thin-layer chromatograms. Chromatography of the unaltered extracts with 2,6- dichlorobenzoic acid revealed identical Rf-values. The esterified aqueous extracts chromatographed precisely with methyl-2,6-dichlorobenzoate. Gas chromatography of the 14C-labelled compound with an Rf of 0.95 exhibited a retention time identical to that of methyl-2,6-dichlorobenzoate. The quantity of 14C-labelled compound that chromatographed as 2,6-dichlorobenzoate increased with time of exposure of the various test organisms to dichlobenil -14C. Other metabolites of dichlobenil -14C. were not resolved by these chromatographic procedures. (author)

1966-01-01

197

Fractionation of phosphorus added as a vegetal residue (32 P) and a fertilizer (32 P) between soil, plant and microbial biomass  

International Nuclear Information System (INIS)

Sugar cane straw and/or P-fertilizer phosphorus-32 labelled were added to a Red Yellow podzolic soil from Goiana-PE. The treated samples were used in a pot experiment, growing sorghum plants for 4 and 6 weeks, and in an incubation experiment with incubation periods of 1, 2, 3, 4 and 6 weeks without plants in order to follow the dynamics of the P added. After each harvest and incubation period the soil were analysed for 31 P and 32 P in the microbial biomass and in sequential extracts with resin (Pi), 0.5 M Na H Co3 (Pi, Po) and 0.1 N NaOH (Pi, Po). The 31 P and 32 P contents of the sorghum in the pot experiment were also determined. (author).

1988-01-01

198

Atmospheric 14C and century-scale solar oscillations  

International Nuclear Information System (INIS)

Here we show from maximum-entropy spectral analysis of a 9,600-yr high-precision 14C chronology that changes occur in the Sun's convective zone with a fundamental oscillatory mode of about 2.4 X 10-3 yr-1 (420-yr period). We also identify several harmonics. Previous searches for cyclicity in the atmospheric 14C record have yielded periods near 140 and 200 yr. We discuss the implications of a longer and more precise 14C record. (author).

1989-01-01

199

Asymmetric synthesis of 14C-labeled L-propargylglycine  

International Nuclear Information System (INIS)

[en] L-Propargylglycine was efficiently labeled with 14C at the 1- and 2- positions. The synthesis was achieved according to the scheme illustrated in Fig.1. Ni-complex of Schiff's base, Gly-Ni-BPB(4) was derived from [U-14C]glycine(2) and (S)-BPB(3). Diastereoselective alkylation of 4 with propargyl bromide followed by acid hydrolysis afforded L-propargyl[1, 2-14C]glycine(1). The optical purity of 1 was proved to be 97.0% and the overall yield of 1 from 2 was 28.2%. (author)

2010-01-01

200

Atmospheric /sup 14/C and century-scale solar oscillations  

Energy Technology Data Exchange (ETDEWEB)

Here we show from maximum-entropy spectral analysis of a 9,600-yr high-precision /sup 14/C chronology that changes occur in the Sun's convective zone with a fundamental oscillatory mode of about 2.4 X 10/sup -3/ yr/sup -1/ (420-yr period). We also identify several harmonics. Previous searches for cyclicity in the atmospheric /sup 14/C record have yielded periods near 140 and 200 yr. We discuss the implications of a longer and more precise /sup 14/C record.

Stuiver, M.; Braziunas, T.F.

1989-03-30

 
 
 
 
201

Hemisynthesis of [3`-{sup 14}C]-Taxotere  

Energy Technology Data Exchange (ETDEWEB)

A new route to semisynthetic Taxotere, 1, is described using the mixed anhydride obtained from 2,4,6-trichlorobenzoyl chloride and [3-{sup 14}C]-cinnamic acid 6, for the esterification of 7,10-O-diTroc-10-deacetylbaccatin III, 4. Hydroxyamination on the unsaturated C-2`-3` deprotection of the C-7,10-Troc groups of the ester 7 gave taxotere 1. [3`-{sup 14}C]-Taxotere 9 (specific activity : 50 MCi/mmol) was obtained from [3-{sup 14}C]-cinnamic acid 5` with a 5% yield. (Author).

Suzzoni, S. de; Boullais, C.; Vandais, A.; Noel, J.P. [CEA Centre d`Etudes Nucleaires de Saclay, 91 - Gif-sur-Yvette (France). Service des Molecules Marquees

1995-12-31

202

Hemisynthesis of [3'-14C]-Taxotere  

International Nuclear Information System (INIS)

A new route to semisynthetic Taxotere, 1, is described using the mixed anhydride obtained from 2,4,6-trichlorobenzoyl chloride and [3-14C]-cinnamic acid 6, for the esterification of 7,10-O-diTroc-10-deacetylbaccatin III, 4. Hydroxyamination on the unsaturated C-2'-3' deprotection of the C-7,10-Troc groups of the ester 7 gave taxotere 1. [3'-14C]-Taxotere 9 (specific activity : 50 MCi/mmol) was obtained from [3-14C]-cinnamic acid 5' with a 5% yield. (Author).

1995-01-01

203

Synthesis of 14C-labelled monoacidic metabolites of dithiopyr  

International Nuclear Information System (INIS)

Convenient methods for the selective hydrolysis of the thioester groups of dithiopyr to the corresponding carboxylic acid are described. 2-(Difluoromethyl)-4-(2-methylpropyl)-5-[(methylthio)carbonyl]-6-(trifluoromethyl)-3-pyridine-4-14C-carboxylic acid was obtained by simple potassium hydroxide hydrolysis of 14C-labelled dithiopyr. A more involved strategy was necessary for the preparation of the isomeric carboxylic acid 6-(difluoromethyl)-4-(2-methylpropyl)-5-[(methylthio)carbonyl]-2-(trifluoromethyl)-3-pyridine-4-14C-carboxylic acid. (author).

1991-01-01

204

[Incorporation of 32P-orthophosphate in aortic phospholipids in chronic lathyrims in the rat  

UK PubMed Central (United Kingdom)

Study of 32P orthophosphate incorporation into rat aortic phospholipids after B.A.P.N. treatment, which was followed or not by a lipid-supplemented diet (4.5 p. 100 cholesterol and 37 p. 100 butter). The 32P orthophosphate turnover in the total phospholipid was twice as slow among B.A.P.N.-treated rat than among control-rat. Such a decrease of the 32P orthophosphate turnover could be found as well in phosphatidylethanolamines, phosphatidylcholines and lysophosphatidylcholines. Sphingomyelins showed a very weak metabolic activity among two groups of rats. 32P orthophosphate incorporation into inositolphosphatides was slowed down by B.A.P.N. treatment. Maximal specific activity of inositolphosphatides after injection of 32P orthophosphate was reached within 4 hours in control rats, and 8 hours in B.A.P.N.-treated rats. Three months after the end of B.A.P.N. treatment, no difference in specific activity could be found between control-rats and B.A.P.N.-treated rats. Lipid supplemented diet modified the metabolic activity of phospholipids equally in control rats and B.A.P.N.-treated rats. Specific activity decrease in total phospholipids, phosphatidylcholines, phosphatidylethanolamines, and increase in lysophosphatidylcholines were noted in both groups of animals under a high rat diet. These modifications of the metabolic activity of different phospholipids were most marked fourteen hours after 32P orthophosphate injection. B.A.P.N. treatment only slowed the incorporation of 32P phosphate into phospholipids. Administration of a single lipid supplemented diet or a diet associated with B.A.P.N. treatment induced a slower turnover of phosphatidylcholines and phosphatidylethanolamines, and a faster turnover of lysophosphatidylcholines.

Dousset JC; Soula G; Douste-Blazy L; Pieraggi MT; Julian M; Bouissou H

1976-10-01

205

Bioaccumulation factor for 32P measured in bluegill, Lepomis macrochirus, and catfish, Ictalurus punctatus  

International Nuclear Information System (INIS)

The ratio of the bioaccumulation factors for 32P and phosphorus was determined for edible tissue in two species of freshwater fish by measuring the specific activity (32P activity per milligram phosphorus) in muscle relative to feed. The 32P tracer was added to the feed at a uniform level throughout the study. Feeding was at two levels: ad libitum and at a lower but constant intake per body weight. In the main experiment, bluegill were maintained in a large flow-through tank and sacrificed at approximately weekly intervals for 51 d of 32P accumulation and 28 d of depuration to compare the specific activity with values predicted with a calculational model. In experiments performed in smaller aquaria, the specific activity in bluegill and catfish muscle was compared at two feeding levels and two temperatures. In addition, unfed fish were exposed to 32P in water at a known specific activity to determine the extent of phosphorus uptake directly from water. The pattern of specific activity increase and decrease in fish muscle during the accumulation/depuration experiment was consistent with a one-compartment model, so that specific activity ratios at steady state could be predicted from measurements during relatively brief exposures. On this basis, the ratio of the bioaccumulation factors of 32P and phosphorus in fish feeding ad libitum was 0.081 for bluegill and 0.17 for catfish. Hence, at a mean phosphorus bioaccumulation factor of 70,000, the factors for 32P are 6000 and 12,000, respectively. The ratios were less at lower phosphorus intakes associated with lower feeding rates; moreover, the lesser value for bluegill occurred at a much lower phosphorus intake than by catfish

1987-01-01

206

Bioaccumulation factor for /sup 32/P measured in bluegill, Lepomis macrochirus, and catfish, Ictalurus punctatus  

Energy Technology Data Exchange (ETDEWEB)

The ratio of the bioaccumulation factors for /sup 32/P and phosphorus was determined for edible tissue in two species of freshwater fish by measuring the specific activity (/sup 32/P activity per milligram phosphorus) in muscle relative to feed. The /sup 32/P tracer was added to the feed at a uniform level throughout the study. Feeding was at two levels: ad libitum and at a lower but constant intake per body weight. In the main experiment, bluegill were maintained in a large flow-through tank and sacrificed at approximately weekly intervals for 51 d of /sup 32/P accumulation and 28 d of depuration to compare the specific activity with values predicted with a calculational model. In experiments performed in smaller aquaria, the specific activity in bluegill and catfish muscle was compared at two feeding levels and two temperatures. In addition, unfed fish were exposed to /sup 32/P in water at a known specific activity to determine the extent of phosphorus uptake directly from water. The pattern of specific activity increase and decrease in fish muscle during the accumulation/depuration experiment was consistent with a one-compartment model, so that specific activity ratios at steady state could be predicted from measurements during relatively brief exposures. On this basis, the ratio of the bioaccumulation factors of /sup 32/P and phosphorus in fish feeding ad libitum was 0.081 for bluegill and 0.17 for catfish. Hence, at a mean phosphorus bioaccumulation factor of 70,000, the factors for /sup 32/P are 6000 and 12,000, respectively. The ratios were less at lower phosphorus intakes associated with lower feeding rates; moreover, the lesser value for bluegill occurred at a much lower phosphorus intake than by catfish.

Kahn, B.; Turgeon, K.S.; Martini, D.K.; Dunkerly, S.J.; el-Shinawy, R.M.; Wilson, M.D.

1987-10-01

207

Reviewing the Mid-First Millennium BC 14C “warp” using 14C/bristlecone pine data  

International Nuclear Information System (INIS)

AMS-based 14C measurements have been obtained on 53 dendrochronologically-dated Bristlecone pine (Pinus longaeva) wood samples in decadal increments spanning 2300–2750 cal BP with particular interest focused on the decades centered on 2405 cal BP and 2625 cal BP. In general, there is overall agreement with the current IntCal04/09 consensus calibration curve for this period. For the 2400–2410 cal BP interval, our Bristlecone-based 14C value is consistent with the 14C value obtained by Belfast on Irish oak for that decade but not on German oak values obtained by Seattle. Our 14C value for the 2620–2630 cal BP interval is consistent with the German oak (Heidelberg)-based 14C decadal value. The 10Be-based reconstruction of 14C variations over the 2620–2630 cal BP interval also supports the Heidelberg data although clearly additional clarifications are required before the current tree ring-based 14C and ice core-based 10Be data for this interval can be accurately interpreted and valid inferences obtained.

2013-01-01

208

Thermophilic anaerobic biodegradation of (/sup 14/C)lignin, (/sup 14/C)cellulose, and (/sup 14/C)lignocellulose preparations  

Energy Technology Data Exchange (ETDEWEB)

Thermophilic (55/sup 0/C) anaerobic enrichment cultures were incubated with (/sup 14/C-lignin)lignocellulose, (/sup 14/C- polysaccharide)lignocellulose, and kraft (/sup 14/C)lignin prepared from slash pine, Pinus elliotti, and /sup 14/C-labeled preparations of synthetic lignin and purified cellulose. Significant but low percentages (2 to 4%) of synthetic and natural pine lignin were recovered as labeled methane and carbon dioxide during 60-day incubations, whereas much greater percentages (13 to 23%) of kraft lignin were recovered as gaseous end products. Percentages of label recovered from lignin-labeled substrates as dissolved degradation products were approximately equal to percentages recovered as gaseous end products. High-pressure liquid chromatographic analyses of CuO oxidation products of sound and degraded pine lignin indicated that no substantial chemical modifications of the remaining lignin polymer, such as demethoxylation and dearomatization, occurred during biodegradation. The polysaccharide components of pine lignocellulose and purified cellulose were relatively rapidly mineralized to methane and carbon dioxide; 31% to 37% of the pine polysaccharides and 56% to 63% of the purified cellulose were recovered as labeled gaseous end products. An additional 10% to 20% of the polysaccharide substrates was recovered as dissolved degradation products. Overall, these results indicate that elevated temperatures can greatly enhance rates of anaerobic degradation of lignin and lignified substrates to methane and low-molecular-weight aromatic compounds.

Benner, R.; Hodson, R.E.

1985-10-01

209

14-C-NAPHTHYL, 14-C-METHYL AND 14-C-CARBONYL CARBARYL DISTRIBUTION IN THE PREGNANT MOUSE AND RAT  

Science.gov (United States)

The distribution of carbaryl labeled with 14C in the ring, methyl or carbonyl groups was determined in pregnant mice and rats. Three identical concurrent experiments were performed using each of the three radiolabeled compounds in each of the species so that the different moietie...

210

Forensic applications of 14C bomb-pulse dating  

International Nuclear Information System (INIS)

[en] After a brief review of the basics of 14C bomb-pulse dating, this paper presents two unique forensic applications. Particular attention is dedicated to the use of the 14C bomb-pulse to establish the time of harvest of illicit drugs such as heroin and opium. Preliminary measurements of 14C concentrations in milligram samples taken from seized drugs are presented. 14C bomb-pulse dating can determine whether drug distribution originates from stockpiles or recent manufacture, and support the action of law enforcement authorities against criminal organisations involved in drug trafficking. In addition, we describe the dating of wine vintages for a number of authenticated single label vintage red wines from the Barossa Valley - South Australia. Our results show that radiocarbon dating can be used to accurately determine wine vintages and therefore reveal the addition of unrelated materials of natural and synthetic origin

2004-01-01

211

Microanalytical 14C AMS measurements on carbonaceous particles  

International Nuclear Information System (INIS)

[en] Carbonaceous particles are increasingly recognized as an important atmospheric constituent. These small atmospheric particles, including black carbon (BC) and organic carbon (OC), are produced by incomplete combustion of fossil fuels, biofuels and outdoor biomass. It has been suggested, that climate forcing by BC contributes substantially to global warming although OC might partly reduce warming effects due to indirect effects such as cloud brightness and cloud cover. Current investigations aim at a partitioning of BC and OC to biogenic and anthropogenic sources to improve our knowledge on the contribution of each fraction to the ambient atmosphere. One approach to solve this problem is a determination of the 14C concentration. The 14C activity in ambient, biogenic carbon is 227 Bq/kg C and the corresponding atomic ratio for 12C/14C approx. 1012:1. OC and mainly BC are also produced in fossil fuel burning. In this fraction 14C is extinct. (orig.)

2004-09-03

212

Whole body autoradiography with mice using 14C-thymidine  

International Nuclear Information System (INIS)

Whole body autoradiography was performed using common histology equipment. Results were useful with some restrictions. 14C-thymidine and/or itsmetabolites were found in those tissues with high rate of mitosis. (Author).

1982-01-01

213

Determination of extractable and nonextractable radioactivity from small field plots 45 and 95 weeks after treatment with [14C]dicamba, (2,4-dichloro[14C]phenoxy)acetic acid, [14C]triallate, and [14C]trifluralin  

International Nuclear Information System (INIS)

The degradation of ring-labeled [14C]dicamba (2-methoxy-3,6-dichlorobenzoic acid), ring-labeled [14C]-2,4-D [(2,4-dichlorophenoxy)acetic acid], [2-14C]triallate [S-(2,3,3-trichloroallyl) diisopropylthiocarbamate], and ring-labeled [14C]trifluralin (?,?,?-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine) was studied under field conditions at rates of 1 kg/ha in small sandy loam plots. Duplicate plots were sampled to a depth of 10 cm after 45 and 95 weeks and extracted with aqueous acetonitrile to determine amounts of extractable radioactivity. The extracted soils were then oxidatively combusted to determine nonextractable, or bound, radioactivity. After 45 weeks, soluble radioactivity recovered from the dicamba-, 2,4-D-, triallate-, and trifluralin-treated plots was

1984-01-01

214

Studies on residues of 14C malathion in soils  

International Nuclear Information System (INIS)

The extractability, the mobility and formation of bound 14C labelled residues in two soils under normal conditions were investigated with malathion. Comparison is made between irradiated and autoclaved soils. The highest concentration of the product is found in the first section (0-4cm) after experiment. Three compounds were found in the soils. 14C malathion is decomposed to 14C02. The degradation is more important in organic matter rich soil. In rich soil, 50% of 14C product is degradated after 17 days. This percentage is only 10%, for poor soil. The 14CO2 produced in non-sterile soil is 33,05%. This percentage is respectively 10,92% and 3,57% only for irradiated and autoclaved soils

1991-01-01

215

Synthesis of tetramethylethylenedioxydi-[[sup 14]C]-methylsilane  

Energy Technology Data Exchange (ETDEWEB)

The synthesis of tetramethylethylenedioxydi-[[sup 14]C]-methylsilane, a molecule used as a plasticizer in polysiloxane/silica blends, is described. This compound was synthesized in a 48% overall radiochemical yield from [[sup 14]C]-methyl iodide and with a specific radioactivity of 33 [mu]Ci/mmole. It was used to study the competitivity of adsorption of plasticizers and models of polydimethylsiloxanes towards silicas. (author).

Vidal, A.; Joachim, F.; Papirer, E. (Centre National de la Recherche Scientifique (CNRS), 68 - Mulhouse (France). Centre de Recherches sur la Physico-Chimie des Surfaces Solides)

1990-04-01

216

Method of preparing (U-14C)-cellobiose  

International Nuclear Information System (INIS)

(U-14C)-cellobiose is prepared from whole Chlorella algae cells cultivated in a 14CO2 medium. A cellulose enzyme solution buffered (e.g., with a citrate-phosphate buffer) to pH=4.5 to 6.0 is used to treat the cells. The released (U-14C)-cellobiose in the solution is separated by paper chromatography. (E.S.).

1980-02-29

217

Synthesis of the 14C-labelled juvenoid W 328  

International Nuclear Information System (INIS)

The labelled juvenoid 2-(4-(2-(ethoxycarbamatoethoxy)(benzene-U-14C)benzyl-1-cycloh exanone ethylene acetal(8) (W 328) was prepared starting from p-hydroxy(ring-U-14C)benzoic acid (1) by a seven step synthesis in 11% overall yield. The reaction conditions previously reported for the ''cold'' synthesis of the W 328 (8) were modified to suit the small scale preparation. (Author)

1994-01-01

218

Synthesis of [1-14C] palmitic acid  

International Nuclear Information System (INIS)

The synthesis of [1-14C] palmitic acid via Grignard Reaction is reported. The carbon-C14 dioxide was liberated by dropping sulfuric acid onto barium carbonate-C14. The yield of [1-14C] palmitic acid was 44.8%. A radiochemical purity of more than 99.5% was determined by HPLC and the product was proved to be free of impurity by TLC.

1997-01-01

219

Genetic consequences of the 14C-decay  

International Nuclear Information System (INIS)

There are only few investigations on the biological and genetic consequences of 14C decay especially due to the long half-life of 14C. Naturally, the consequences of the decay of 14C incorperated in DNA is of particular interest. Due to the thus occuring conversion into nitrogen, either opening of the ribose residue ring and base occurs or conversion into base analogue. The present work gives a survey on the investigational results on this topic which were obtained by various authors on phages, bacteria, algae and animal cells, as well as on molecular level. It was shown that the transmutation for the occurence of a lethal effect is many times more effective than ?-radiation. Investigations of the mutagenic effects and other genetic effects of the 14C decay were carried out on micro-organisms, however also on higher forms of life, plants, insects and mice. An analysis of the mutations showed that transitions as well as transversions occur. As example for genetic effects of 14C decay, the two following are named. In the case of mice which were exposed to 14CO2 atmosphere, it was seen that the number of polyptoidal chromosome sets in the first spermotacytes increased considerably, an increase of the chromosome conversion rate was however not observed. When treating gryllus larvae with 14C, it was interesting to note that the crossing of 14C-contained females with normal males produced viable eggs, whereas normal females after crossing with 14C contained males, laid no more eggs. (MG) 891 MG.

1978-01-01

220

14C-Metampicillin stability in several physiological sera  

International Nuclear Information System (INIS)

Degradation of 14C-metampicillin incorporated to several physiological sera for medical uses has been studied. Influence of environmental conditions as well as possible interaction with the solvent have been specially analyzed. Degradation level of the labelled multiplication has been determined and its degradation products have been separated by using chromatographic and radiochemical methods. Likewise, the 14C-metam picill synthesis has been described. Finally, the results obtained have been discussed and evaluated. (Author) 9 refs.

1981-01-01

 
 
 
 
221

Design and construction of a prototype for the obtention of {sup 32}P; Diseno y construccion de un prototipo para la obtencion de {sup 32}P  

Energy Technology Data Exchange (ETDEWEB)

The {sup 32} P are a pure emitting radioisotope of maximum energy of 1.71 MeV with half life of 14.28 days that he has applications in the industry, in agriculture, in medicine, in biology and in ecology (6,11,12,13,17,21,22,23,24,25,26,27,28,29,30,31,32 33).The {sup 32} P can be used in the industry like radiotracer in the investigation of some operations and processes and as element of measurement of some industrial meters. In agriculture is used as radiotracer (29) in the investigation of diverse biological processes that have to do with the production of diverse nutritious products. In medicine has uses but very therapeutic mainly in the treatment of some become cancerous (28, 31, 25, 27) in the diagnosis of blood disorders (24) and like part of materials of production of aortic prosthesis (6). The {sup 32} P are also used in the molecular investigation in biology and in genetics (33), and in studies of ecosystems (32) and of DNA (22). One can obtain the {sup 32} P by means of diverse nuclear reactions depending on the material used as matter it prevails, since it doesn't exist in the nature. But anyone in the ways of obtaining it it should imply a process of radiochemical separation that involves so many steps like they are required, depending on the material used as matter prevails, of the purity with which he wants himself to obtain and of the resources that it has available. The objective of this work was design, to build and to prove a prototype to obtain the {sup 32} P to leave of the irradiation of S {alpha} with fast neutrons at experimental level, which implies also to design a process that contemplates diverse stages and procedures for each one of them. The process was outlined in five stages: matter purification prevails, preparation of irradiation capsules, irradiation of irradiation capsules, transport and opening capsules of irradiation and radiochemical separation. In the last stage it was where uses the prototype of radiochemical separation in basis to the outlined process and considering aspects of environmental and personal radiological safety, in such a way that the radiochemical separation carried out in two stages in the same prototype and with the less manipulation of the radioactive material.To know the functionality of the process and of the prototype, they were carried out three tests of these, in each one of those which the stages of the process were evaluated by means of mensuration of physicochemical parameters and by means of characterizations of the materials involved in these stages with diverse technical analytic instrumental: X-ray diffraction, X-ray fluorescence, scanning electron microscopy, high-purity Ge semiconductor detector proportional detector proportional of low bottom gas and liquid scintillation detector. The results of the process for stages showed that with the outlined method of purification of the matter prevails six of nine constituent elements they were removed of chemical sludges of the raw materials of the process, S {alpha}, and that with the process and the prototype of radiochemical separation it was reached to separate {sup 32} P of the S {alpha}. In general, it was reached to obtain {sup 32} P from S {alpha} irradiated with relatively small quantities of radiochemical impurities. (Author)

Duarte A, C

2003-07-01

222

Preparation of (N-methyl-14C) Erythromycin and (N-methyl-14C) Erythromycin cyclic carbonate  

International Nuclear Information System (INIS)

[en] (N-methyl-14C)Erythromycin was obtained by methylation of des-N-methylerythromycin with (14C)formaldehyde in the presence of Pd-catalyst. Radiochemical yield reached 42.3%, radiochemical purity 95.1%. Condensation of this compound with ethylene carbonate led to (N-methyl-14C)erythromycin cyclic carbonate with radiochemical yield of 71% and radiochemical purity of 98.5%. Both products were purified on a chromathografic column with aluminium oxide or silica gel bed

1982-01-01

223

Clinical investigation of 14C-urea breath test  

International Nuclear Information System (INIS)

To investigate clinical value of 14C-urea breath test (14C-UBT) for the diagnosis of Helicobacter pylori(Hp), 70 patients were both performed gastroscopy (taking gastric mucosae biopsy for rapid urease test and histology) and 14C-UBT (some patients by Hp-IgG or DNAHp test also) within two days. The positive cases of both rapid urease test and histology was defined as 'gold standard' of Hp-positive, whereas the negative cases of both rapid urease test and histology as 'gold standard' of Hp-negative. The sensitivity of 14C-UBT was 93.2%, the specificity 73.1%, and the diagnostic accuracy 85.7%. The difference (comparing with 'gold standard') was not marked (x2 = 0.90.05(1)2 = 3.84, P>0.05). But the diagnostic accuracy of 14C-UBT (85.7%) and Hp-IgG (50%) had a marked difference (x2 13.80>x0.01(1)2 = 6.64, P14C-UBT was easy to operate, reliable and suitable for clinical application

2000-01-01

224

Bioevaluation of {sup 32}P patch designed for the treatment of skin diseases  

Energy Technology Data Exchange (ETDEWEB)

Objective: The objective of this study was to design and evaluate a {sup 32}P patch for the treatment of skin diseases. Materials and Methods: The patch was prepared from chromic phosphate {sup 32}P and silicone. Bioelimination and biodistribution in healthy and treated animals, and the therapeutic efficacy of two treatment schemes (single dose and fractionated dose) in an animal model of skin cancer were studied. Results: Based on the bioelimination and biodistribution studies, no leakage of {sup 32}P from the patch was observed. The treated tumors reduced their mean diameter compared to controls. The single-dose therapeutic scheme showed a higher number of complete and partial remissions compared to the fractionated scheme. These results were confirmed by histopathological analysis of the samples. Conclusion: The {sup 32}P patch was designed and produced according to specifications for the treatment of superficial lesions of the skin. Although the {sup 32}P patch is an open source, it behaves like a sealed one for use in brachytherapy treatments.

Salgueiro, Maria Jimena [Radioisotope Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires, 1113 Buenos Aires (Argentina)], E-mail: jsalgueiro@ffyb.uba.ar; Duran, Hebe [Radiobiology Department, National Energy Atomic Commission, 1150 Buenos Aires (Argentina); Palmieri, Monica [Biodiversity and Experimental Biology Department, School of Exact and Natural Sciences, University of Buenos Aires, 1113 Buenos Aires (Argentina); Pirchio, Rosana [Dosimetry Department, National Energy Atomic Commission, 1150 Buenos Aires (Argentina); Medina, Vanina [Radioisotope Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires, 1113 Buenos Aires (Argentina); Ughetti, Ricardo [Research and Development Department, Laboratorios Bacon SAIC, 1603 Buenos Aires (Argentina); Croci, Maximo [Institute of Immunooncology, Dr. E. Crescenti, 1187 Buenos Aires (Argentina); Nicolini, Jorge [Research and Development Department, Laboratorios Bacon SAIC, 1603 Buenos Aires (Argentina); Zubillaga, Marcela [Radioisotope Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires, 1113 Buenos Aires (Argentina)

2008-02-15

225

Bioevaluation of 32P patch designed for the treatment of skin diseases  

International Nuclear Information System (INIS)

Objective: The objective of this study was to design and evaluate a 32P patch for the treatment of skin diseases. Materials and Methods: The patch was prepared from chromic phosphate 32P and silicone. Bioelimination and biodistribution in healthy and treated animals, and the therapeutic efficacy of two treatment schemes (single dose and fractionated dose) in an animal model of skin cancer were studied. Results: Based on the bioelimination and biodistribution studies, no leakage of 32P from the patch was observed. The treated tumors reduced their mean diameter compared to controls. The single-dose therapeutic scheme showed a higher number of complete and partial remissions compared to the fractionated scheme. These results were confirmed by histopathological analysis of the samples. Conclusion: The 32P patch was designed and produced according to specifications for the treatment of superficial lesions of the skin. Although the 32P patch is an open source, it behaves like a sealed one for use in brachytherapy treatments

2008-01-01

226

Insulin and thyroxine effect on 32P incorporation in the phospholipids of chicken intestinal mucosa  

International Nuclear Information System (INIS)

Trials were conducted with 56-day-old broiler chickens. The effect was followed up of insulin and alloxan as well as of L-thyroxine and 6-methylthiouracil on 32P incorporation in phospholipids of the duodenal mucosa. A segment of the duodenum was isolated and Na2H32PO4 was introduced therein. The lipids were extracted from duodenal mucosa and the individual phospholipids were separated by means of thin layer chromatography on sillica gel-G. Radioactivity was determined of individual phospholipid fractions. Blood glucose level was studied in insulin and alloxan-treated chickens. The inference was drawn that insulin significantly enhances 32P incorporation in the phospholipids in broiler chicken duodenal mucosa. The drop in blood glucose in insulin-treated chickens is inversely proportional to 32 P inclusion in individual phospholipids of duodenal mucosa. L-thyroxine exerts positive effect in chickens concerning 32P incorporation in lecithin and lysolecithin, this effect being negative with respect to sphingomyelin, cephalin and cardiolipin. Thyroid gland inhibition by 6-methylthiouracil induces negligible decline in 32P inclusion. (author).

1977-01-01

227

Use of colloids of chromic [32P] phosphate in treatment of solid tumors  

International Nuclear Information System (INIS)

In order to evaluate the effectiveness of an intratumorally single dose of chromic [32P] phosphate for the treatment of solid tumors, studies of bioelimination, biodistribution, and therapeutic action were carried out. Only for comparative purposes were similar studies undertaken using a solution of sodium [32P] orthophosphate-gelatin. Results show that when sodium [32P] orthophosphate-gelatin was intratumorally injected, the percentage of total elimination, after 32 days of treatment, was equal to 85.90 ± 8.70%, with a higher percentage in urine (64.50 ± 13.70%) than in feces (21.40 ± 4.50%). In biodistribution studies, the greater percentage was found in bone (15.54 ± 2.21%), whereas only 2.51 ± 0.39% remained in the tumor. When chromic [32P] phosphate was intratumorally injected, we found that the total elimination was equal to 51.70 ± 6.90%, with a higher amount in feces (32.70 ± 4.80%) than in urine (19.00 ± 3.60%). Biodistribution studies demonstrated that 28.93 ± 1.30% was still in the tumor and 19.01 ± 1.30% of the injected activity was found in the liver. On the other hand, when therapeutic action was evaluated, no tumoral regression was observed. These results demonstrate that the colloid of chromic [32P] phosphate cannot be used in the treatment of solid tumors as it mobilizes from the injection point, delivering a high dose to the entire organism.

1996-01-01

228

sup 14 C-sulfur mustard adducts of calf thymus DNA. Final report, Aug-Sep 90  

Energy Technology Data Exchange (ETDEWEB)

A grant was awarded to TNO-PML to develop immunochemical monitoring systems for the detection of DNA-HD and Protein-HD adducts in humans following exposure to HD. TNO-PML has been using 35S-HD to prepare adducts for their assays, which have inherent shortcomings that limit detection sensitivity. An experimental batch of 14C-HD-DNA adducts was prepared in an attempt to increase the assay sensitivity. Double - and single-stranded purified calf thymus DNA preparations were reacted with 142, 14.2 and 1.42 uM of 14C-HD under aqueousfree conditions. The 14C-HD-DNA adducts were isolated at -20C in 75% ethanol solution and freed of HD agent and organic solvents (i.e., acetone and alcohol). The 14C-HD-DNA adducts in aqueous buffer were analyzed for specific activity and purity. The ds-DNA-HD adducts were uncontaminated, but the ss-DNA-HD adducts were initially slightly contaminated with alcohol.

Yaverbaum, S.

1991-02-01

229

{sup 14}C in human diet in three Spanish locations; {sup 14}C en dieta humana en tres localizaciones espanolas  

Energy Technology Data Exchange (ETDEWEB)

{sup 14}C reach humans via the diet. In this study we present the results of diet samples from three locations type Spanish, Seville, Caceres and Tenerife. These diets are collected quarterly and historical dates back to early 2008.

Gomez Martinez, I.; Santos Arevalo, F. J.; Manjon, G.; Garcia-Tenorio, R.

2011-07-01

230

Synthesis of [14C]-62514, a radiolabelled derivative of erythromycin A, via [2-14C]N,N-dimethylethylenediamine  

International Nuclear Information System (INIS)

[en] The synthesis of [14C]A-62514, 11-deoxy-11[carboxy(2-dimethylamino-[1-14C]ethyl)amino]-6-0-methyl-e rythromycin A 11,12-(cyclic ester), was performed in five steps. The key intermediate, [2-14C]N,N-dimethylethyl-enediamine, was obtained in 80% yield by reacting Eschenmoser's salt with K14CN and reducing the resulting [1-14C]N,N-dimethylglycinonitrile with H2 and Raney Ni in methanol and ammonium hydroxide. The final product was obtained 97% radiochemically pure in an overall radiochemical yield of 14%, with a specific activity of 39 mCi/mmol. (author)

1991-01-01

231

Intramolecular labelling of sucrose made by leaves from [14C] carbon dioxide or [3-14C] serine  

International Nuclear Information System (INIS)

Pea leaves were illuminated in air containing 150 or 1000 p.p.m. of 14CO2 for various times. Alternatively segments of wheat leaves were supplied with [3-14C]serine for 40 min in the light in air with 145, 326 or 994 p.p.m. of 12CO2. Sucrose was extracted from the leaf material, hydrolysed with invertase, and 14C in the pairs of carbon atoms C-3 + C-4, C-2 + C-5 and C-1 + C-6 in the glucose moiety was measured. The results obtained after metabolism of 14CO2 were consistent with the operation of the photosynthetic carbon-reduction cycle; the effects of CO2 concentration on distribution of 14C in the carbon chain of glucose after metabolism of [3-14C]serine is more easily explained by metabolism through the glycollate pathway than by the carbon-reduction cycle. (author).

1978-01-01

232

Chemical synthesis of high specific-activity (/sup 35/S)adenosylhomocysteine  

Energy Technology Data Exchange (ETDEWEB)

The study of the family of transmethylases, critical to normal cellular function and often altered in cancer, can be facilitated by the availability of a high specific-activity S-adenosylhomocysteine. The authors report the two-step preparation of (/sup 35/S)adenosylhomocysteine from (/sup 35/S)methionine at a specific activity of 1420 Ci/mmol in an overall yield of 24% by a procedure involving demethylation of the (/sup 35/S)methionine to (/sup 35/S)homocysteine followed by condensation with 5'-chloro-5'-deoxyadenosine. The ease of the reactions, ready availability and low cost of the reagents and high specific-activity and stability of the product make the procedure an attractive one with many uses, and superior to current methodology.

Stern, P.H.; Hoffman, R.M.

1986-11-01

233

Inter-well tracer test method for 35S-KSCN isotope tracer in oil field  

International Nuclear Information System (INIS)

[en] The isotope tracer 35S-KSCN and its trace test in oil field were described.An extraction method for 1 000 mL 35S-KSCN sample from oil field were investigaged. The exper-iment indicated that the microamount 35S-KSCN could be extracted quantitatively with TBP at the range of 0.5-2.0 mol/L HCl. The activity of 35S-KSCN was determined with a liquid scintillation counter,the minimum detectable limit was 0.02 Bq/L, the recovery rate of this method was more than 90%, the linearity relation from 0.02 Bq/L to 5 Bq/L was good, R2= 0.999 5. The results showed that the tracer was a steady tracer in water flood reserves. This method is simple and rapid, and it can be used in the inter-well tracer test technology. (authors)

2009-01-01

234

35S induced dominant lethals in male germ cells of mouse  

International Nuclear Information System (INIS)

(CBA female x C3H/He male) F1 males born to 35S (20 ?Ci) treated animals during major organogenesis period were tested for dominant lethal mutations at maturity. The pre-implantation loss showed an increase from 6.88% in the control to 10.92% in 35S treated animals. Similarly the post-implantation loss has increased from 3.96% (control) to 7.40%. As a result of the increased pre- and post-losses the total loss showed a significant increase (17.51%) in F1 males born to 35S treated animals when compared to controls (10.57%). Thus the results clearly show that 35S is mutagenic in male germ cells of mouse. (author)

1976-12-18

235

Laboratory and field studies with 32P labeled Toxorhynchites rutilus rutilus  

International Nuclear Information System (INIS)

Females and eggs of Toxorhynchites r. rutilus were labeled with 32P by feeding fourth-stage larvae 32P labeled Aedes aegypti larvae. Eggs from females up to 3 weeks in age had detectable levels of radioactivity and individual eggs contained ca. 0.3% of the mother's total radioactivity. Comparisons of labeled and unlabeled females in indoor and outdoor cage tests indicated that survival and fecundity of the 2 groups were approximately equal. No differences were noted for dispersal and fecundity of labeled and control females released in field tests. The 32P-labeled Tx. r. rutilus females behave similarly to unlabeled females, and this method of radiolabeling provides a sound tool for tracking laboratory-reared females released into an area with an indigenous population

1986-01-01

236

Laboratory and field studies with /sup 32/P labeled Toxorhynchites rutilus rutilus  

Energy Technology Data Exchange (ETDEWEB)

Females and eggs of Toxorhynchites r. rutilus were labeled with /sup 32/P by feeding fourth-stage larvae /sup 32/P labeled Aedes aegypti larvae. Eggs from females up to 3 weeks in age had detectable levels of radioactivity and individual eggs contained ca. 0.3% of the mother's total radioactivity. Comparisons of labeled and unlabeled females in indoor and outdoor cage tests indicated that survival and fecundity of the 2 groups were approximately equal. No differences were noted for dispersal and fecundity of labeled and control females released in field tests. The /sup 32/P-labeled Tx. r. rutilus females behave similarly to unlabeled females, and this method of radiolabeling provides a sound tool for tracking laboratory-reared females released into an area with an indigenous population.

Smittle, B.J.; Focks, D.A.

1986-12-01

237

Radiotracer studies for determining the active root distribution of Hevea brasiliensis using 32P  

International Nuclear Information System (INIS)

Phosphorus uptake by mature Hevea trees from the soil was studied using 32P tracer. The soil injection of 32P in solution was used. Latex and leaf assay were compared for detecting 32P uptake and latex was found to be more convenient and reliable than leaf assay for determining the distribution of active roots with respect to vertical and lateral distances from the tree. Latex radioactivity tended to be higher at 8 weeks than at 4 weeks. Latex assay made after 8 weeks was therefore used in this study. The activity of the latex was higher when the tracer was placed at a depth of 15 cm. Of the five lateral distances compared, the highest root activity was observed at a distance of 0.75 m from the tree. (author).

1978-12-15

238

Exchange of radioactive phosphorus 32P between the components of an artificial plant community  

International Nuclear Information System (INIS)

In artificial plant communities (wooden cases with soil) the following plants were grown together for one year: case No. 1: Betula verrucosa Ehrh. (2 specimens) and Carex pilosa Scop.; cases No. 2 and 3: Alnus incana (L.) Mnch., Fraxinus excelsior L., Padus avium Mill., Aegopodium podagraria L., Eupatorium cannabinum L. and others. Into the stalks or stems of one to three plants of every case 32P was introduced. After 3 months all the plants in every case contained radiactive phosphorus. There were great differences in 32P concentration (up to ten-fold) between plants grown in the same case. The concentration of 32P in plants into which it had not been introduced was of the range of 0.1%. (author)

1975-01-01

239

Synthetic process for preparation of sup 32 P-labeled nucleotides  

Energy Technology Data Exchange (ETDEWEB)

This patent describes a process for the production of {sup 32}P-labeled NTP. It comprises: reacting H{sub 3} {sup 32}PO{sub 4} with an excess of X-CoA where X is selected from the group consisting of acetyl, propionyl, butyryl and succinyl in the presence of phosphotrancetylase, inducing the formation of {sup 32}PO{sub 4}-labeled X, simultaneously reacting the resulting X-{sup 32}PO{sub 4}, as it is formed, with NDP in the presence of acetate kinase, under conditions capable of both inducing the formation of X-{sup 32}PO{sub 4} and conversion of NDP to {sup 32}P-labeled NTP, and stopping the reactions before excess hydrolysis of {sup 32}P-labeled NTP occurs to prepare the product having a specific activity greater than 7500 Ci/mM.

Di Meo, J.J.

1992-02-11

240

Preparation of (/sup 14/C)uridine 5'-diphosphate and (/sup 14/C)guanosine 5'-diphosphate  

Energy Technology Data Exchange (ETDEWEB)

Procedures have been developed for the routine enzymatic synthesis of (/sup 14/C)UDP and (/sup 14/C)GDP from commercially available enzymes and (/sup 14/C)UMP and (/sup 14/C)GMP. Using high pressure liquid chromatography, the products are recovered in high yield (60-80%) and with high purity. The (/sup 14/C)UDP and (/sup 14/C)GDP are utilized as substrates for ribonucleotide reductase.

Cory, J.G.; Bacon, P.E.

1984-08-01

 
 
 
 
241

Construction of expression vector containing glnA gene and detection of NPT II activity in the transgenic rice calli using 32P-labelled compound  

International Nuclear Information System (INIS)

The glnA gene encoding glutamine synthetase (GS) was amplified from Azospirillum brasilense Sp7 by PCR technique. the amplified 1.4 kb DNA fragment was cloned at the EcoRV site of Bluescript-SK. Both sequencing and restriction digestion data showed that the 1.4 kb DNA fragment flanked with BamHI site at each end was really the glnA gene of A. brasilense Sp7. The glnA gene was ligated with Bg1 II site of pCo24. As a result, an expression vector pGSC35 with CaMV35S promoter was obtained. Using colony in situ hybridization with ?-32P-dATP labelled probes to screen the positive clones, another glnA gene expression vector pAGNB92 with rice actin 1 promoter was constructed after three rounds of ligation and transformation. Protoplasts isolated from rice cell suspension line cv. T986 were transformed with glnA expression vectors pGSC35 and pAGNB92 containing neomycin phosphotransferase II (NPTII) gene by using PEG fusion and electroporation. Transformed microcalli were selected on media containing G418 disulfate salt. NPT II activity was detected in 37% of G418 resistant calli by using dot blot hybridization with ?-32P-ATP and kanamycin as substrate

1993-01-01

242

Development of A 32P-Postlabeling Technic for Detection of the Initiation of Cancer  

International Nuclear Information System (INIS)

It is well know that the interaction between exogenous and also endogenous subs-trances with macromolecular biology (Protein or DNA) in human with in sublethal or lethal level can lead to the initiation of cancer (Auerbach, 1946, Phillips, 1981). In the assessment of carcinogen exposure (exo genus or endo genus), bio markers are chosen based on a knowledge of the internal interactions of carcinogen molecules/metabolites with cellular macromolecules such as DNA, i.e. formation DNA adduct. The 32P-postlabeling assay is most sensitive, fast and applicability methods to structurally diverse classes of chemical. It has been developed to detect DNA adduct (Randerath at. All, 1993, Reddy, 1986). The 32P-Postlabeling technic has emerged as the method of choice for qualitative detection and quantitation of carcinogen-DNA adducts in human. The result of detection of the Adduct will lead the understand of the mechanism reaction of the substance in human organ. The assay of the 32P-Postlabeling involves a stepwise sequence of biochemical reaction entailing: Isolation DNA and following with cleavage by enzymatic hydrolyzed of intact DNA (Nucleotide) with phosphate in 3 position. Attachment of a 32P- label to the 5-hydroxyl end of DNA (Nucleotide) creating a 3,5-biphosphate; following by separation and detection of adducts by high-regulation TLC and autoradiography respectively and quantitation of adducts by measurement of radioactivity. The 32P-Postlabeling was used to detection of DNA adduct of Polycyclic aromatic and alpha, beta unsaturated carbonyl compound such crotonaldehyde, which is in this paper to discussed. We have investigated and developed the 32P-Postlabeling for detection of modified DNA of crotonaldehyde in vitro and in vivo as markers for initiation of cancer. From the result of study were found the adduct the adduct in several organs of F-344 rast after gavage and persisted to a certain extent (Eder dan Budiawan, 1997).

2000-02-23

243

/sup 32/P-postlabeling analysis of non-radioactive aromatic carcinogen--DNA adducts  

Energy Technology Data Exchange (ETDEWEB)

A newly developed enzymatic /sup 32/P-postlabeling method was applied to the analysis of DNA's containing non-radioactive arylamine, arylamide, and polycyclic aromatic hydrocarbon adducts. DNA reacted in vitro with N-hydroxy-2-amino-fluorene, N-acetoxy-2-acetylaminofluorene, and 7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene, respectively, as well as DNA preparations from the liver of rats treated with N-hydroxy-2-acetylaminofluorene and benzo(a)pyrene, respectively, were enzymatically digested to deoxyribonucleoside 3'-monophosphates, which were then converted to (5'-/sup 32/P)deoxyribonucleoside 3',5'-bisphosphates by T4 polynucleotide kinase-catalyzed (/sup 32/P)phosphate transfer from (gamma-/sup 32/P)ATP. The /sup 32/P-labeled nucleotides were resolved by anion-exchange t.l.c. on polyethyleneimine-cellulose and detected by autoradiography. Aromatic adduct nucleotides were found to be retained at the origin in aqueous electrolyte solutions, but to migrate as distinct spots in solvents containing 7-8.5 M urea. Advantage was taken of this observation to remove /sup 32/P-labeled normal DNA nucleotides from adduct nucleotides. This purification enabled the detection of a single adduct in 10(7)-10(8) normal nucleotides. The method appears applicable to the ultrasensitive detection of a large number of carcinogen--DNA adducts of diverse structure without requiring radioactive carcinogens or specific antibodies.

Gupta, R.C.; Reddy, M.V.; Randerath, K.

1982-01-01

244

/sup 32/P-postlabeling analysis of non-radioactive aromatic carcinogen-DNA adducts  

Energy Technology Data Exchange (ETDEWEB)

A newly developed enzymatic /sup 32/P-postlabeling method was applied to the analysis of DNA's containing non-radioactive arylamine, arylamide, and polycyclic aromatic hydrocarbon adducts. DNA reacted in vitro with N-hydroxy-2-amino-fluorene, N-acetoxy-2-acetylaminofluorene, and 7..beta..,8..cap alpha..-dihydroxy-9..cap alpha.., 10..cap alpha..-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene, respectively, as well as DNA preparations from the livers of rats treated with N-hydroxy-2-acetylaminofluorene and benzo(a)pyrene, respectively, were enzymatically digested to deoxyribonucleoside 3'-monophosphates, which were then converted to (5'-/sup 32/P)deoxyribonucleoside 3',5'-bisphosphates by T4 polynucleotide kinase-catalyzed (/sup 32/P)phosphate transfer from (..gamma..-/sup 32/P)ATP. The /sup 32/P-labeled nucleotides were resolved by anion-exchange t.l.c. on polyethyleneimine-cellulose and detected by autoradiography. Aromatic adduct nucleotides were found to be retained at the origin in aqueous electrolyte solutions but to migrate as distinct spots in solvents containing 7-8.5 M urea. Advantage was taken of this observation to remove /sup 32/P-labeled normal DNA nucleotides from adduct nucleotides. This purification enabled the detection of a single adduct in 10/sup 7/-10/sup 8/ normal nucleotides. The method appears applicable to the ultrasensitive detection of a large number of carcinogen-DNA adducts of diverse structure without requiring radioactive carcinogens or specific antibodies.

Gupta, R.C.; Reddy, M.V.; Randerath, K. (Texas Medical Center, Houston (USA))

1982-01-01

245

Correlation Between CT/MRI and Bremsstrahlung SPECT of 32P After Radioembolization of Hepatic Tumors  

Directory of Open Access Journals (Sweden)

Full Text Available Background/Objective: Radioembolization (RE) is a minimally invasive transcatheter therapy through which radioactive microspheres are infused into the hepatic arteries and selectively implanted within the tumor arterioles. Some therapeutic agents are particles incorporating pure ? emitter elements (90Y, 32P) and do not have gamma radiation. Bremsstrahlung imaging of these radiotherapeutic agents confirms distribution of the radiotracer in hepatic tumors or probable extrahepatic deposition of radiopharmaceuticals and helps the physician to predict the patient's response to RE therapy. The aim of this study was demonstration of 32P images and its correlation with CT/MRI findings."nPatients and Methods: Ten patients with variable types of hepatic tumors treated with intra-arterial injection of 32P were included in this study. 24-72 hours after radiotracer administration, bremsstahlung SPECT imaging was performed in all patients with a single head gamma camera equipped with a medium energy collimator. Energy window setting of 100 keV±25% was selected. Reconstructed images were evaluated by two nuclear medicine specialists and one radiologist, and based on compatibility of 32P images with CT/MRI, a grading scale from 1 to 4 was used to express their correlations."nResults: By selecting optimized parameters for bremsstrahlung SPECT images of 32P, we could obtain good quality images. In nine patients, 32P distribution in the liver was correlated with anatomical findings of CT/MRI. "nConclusion: RE is appropriate to deliver high radiation doses to liver tumors with minimal accumulation in the normal liver tissue. Bremsstrahlung imaging is a useful method to confirm proper distribution of the radiotherapeutic agent, which has good correlation with anatomical findings.

M. Amoui; E. Pirayesh; Sh. Akhlaghpoor; M. Khorrami; Sh. Tolooee; H. Poorbeigi; Sh. Sheibani

2010-01-01

246

Metabolism of (/sup 14/C)acetylisoniazid and (/sup 14/C)acetylhydrazine by the rat and rabbit  

Energy Technology Data Exchange (ETDEWEB)

Male rats and rabbits were singly dosed with either 1-(/sup 14/C)acetyl isoniazid (acetylisonicotinoylhydrazine, acetyl-INH, 200 mg/kg po) or 1-(/sup 14/C)acetylhydrazine (50 or 100 mg/kg ip). Urine and expired /sup 14/CO/sub 2/ were collected, and after 6 hr the animals were killed for the analysis of tissue /sup 14/C concentrations and covalent binding of /sup 14/C to hepatic protein. Rats excreted proportionately more /sup 14/C in urine and had lower 14C levels in their tissues compared to rabbits. When acetyl-INH was administered, covalent hepatic protein binding of the acetyl moiety was greater in the rabbit than the rat, but the opposite was observed when acetylhydrazine was administered. Analysis of blood and urine by TLC revealed that the rabbit more rapidly metabolized both acetyl-INH to acetylhydrazine, and acetylhydrazine to diacetylhydrazine than did the rat. These observations suggest that higher amidase activity in the rabbit compared to the rat leads to faster conversion of acetyl-INH to acetylhydrazine which in turn leads to greater covalent binding and hepatotoxicity.

Thomas, B.H.; Whitehouse, L.W.; Zeitz, W.

1984-08-01

247

Measurement of cosmogenic 35S activity in rainwater and lake water.  

UK PubMed Central (United Kingdom)

Although cosmogenic 35S (t(1/2) = 87 d) has been found to be a unique and excellent radioactive tracer for stable S in atmospheric and aqueous environments, its application has been very limited because an analytical method for its detection has not been well-documented. Here, we report a rapid and robust method for analyzing extremely low levels of 35S in rainwater and lake water samples. About 20-L water samples were preconcentrated using an anion exchange column. The purified 35S was precipitated as BaSO4, and the precipitates were collected using a GF/B filter. The 35S in precipitates on the filter was directly counted using a super-low-background liquid scintillation counter with cocktail. We successfully measured 35S in precipitation and lake water samples using this method, which promises future diverse applications of the 35S tracer to S cycling in the environment and to age determination of lake water and shallow groundwater.

Hong YL; Kim G

2005-05-01

248

Labelling of eggs of Ceratitis capitata (Wiedmann) through radioactive sperm ( 32p)  

International Nuclear Information System (INIS)

The labelling of Med-fly eggs, using the sperm in the transmission of radioisotope 32P is described. A hundred hatched couples were used, the males fed on a diet made of 5 g sugar and 1.66g of hydrolized protein. This diet was labelled with a solution of Na2HPO4, in which the atom of phosphorus was labelled with 32P isotope. It showed an activity calculated at 343,9 ?Ci. Statistical treatment of the data indicated that the eggs became labelled and remained labelled until the 5th day after mating, even on eggs laid by female who mated with untreated males

1975-01-01

249

Subnanomole detection and quantitation of high specific activity 32P-nucleotides  

International Nuclear Information System (INIS)

Microbore liquid chromatography utilizes conventional HPLC and ultraviolet detection principles to determine subnanomole mass quantities of biologically significant molecules. This system takes advantage of specifically designed microflow equipment to analyze ultraviolet absorbing species at the picomole range. 32P-labeled nucleotides are examples of compounds routinely used at picomole quantities that are extremely difficult to accurately quantify using standard mass measurement techniques. The procedure described in this paper has the capability of measuring nucleotides down to 10 pmol using commercially available microbore ultraviolet detection equipment. The technique can be used to accurately measure the specific activity of as little as 10 microCi of an aqueous 32P-nucleotide solution.

1991-01-01

250

Synoviorthesis of the knee joint with 32P-chromic phosphate  

International Nuclear Information System (INIS)

[en] 32P-CrPO4 in colloid form has been used for radiosynoviorthesis. There were no acute or subacute side reactions observed. The therapy results were equal to those following 90Y colloid application: 60% very good to good results after 6 months. The substance can be kept on stock since it has a relatively long shelf-life. The cost reduction and the simplification of treatment planning have proved to be the most important advantages of 32P-CrPO4. (orig.)

1979-01-01

251

Studies on degradation and excretion of 32P-methamidophos by lactating cattle, following oral application  

International Nuclear Information System (INIS)

32P-labelled methamidophos (thiophosphoric acid O,S-dimethylester amide) was orally applied to lactating cattle, with the dosage being 2 mg/kg body weight. The half-life of the active agent in blood serum was 10 +- 2 hours. As long as after six hours, up to 1 ppm of extracable 32P activity with components of unmetabolized active agent was recorded in vivo from blood and milk. Active agent was excreted also in urine. Its concentration declined rapidly after 24 hours, though clearly detectable quantities were still present in the circulation. Metabolites were not identified. Binding of methamidophos to proteins and pharmacokinetics measured are discussed in detail. (author)

1986-01-01

252

Wet deposition fluxes of cosmogenic 32P and 33P and variations in the 33P/(32P) ratios at Bermuda  

Science.gov (United States)

A study was undertaken to quantify the supply of cosmogenic 32 P (halflife 14.3 days) and 33 P (halflife 25.3 days) on the island of Bermuda in order to use the natural radioisotopes as tracers of the phosphorus biodynamic in the ocean. The activities of 32 P and 33 P were measured in individual precipitation events over a 1 yr period in 1991-1992 at the Bermuda Biological Station for Research (32° 30'N, 64° 40' W). For the first time, wet deposition rates were determined at a marine site and averaged 8.6 ± 1.5 × 10 -2 dpm cm -2 yr -1 for 32 P and 8.2 ± 2.0 × 10 -2 dpm cm -2 yr -1 for 33 P. The activity ratios in individual precipitation events were found to be remarkably constant, ranging from 0.66 to 1.22. The monthly means of the activity ratio in rainwater showed variations about the annual mean of 0.96. Higher ratios were observed during the combined period March-April 1991 and January-March 1992, contrasting with the lower ratios found during July-November 1991. The individual rain samples of the two contrasting periods belonged to two different populations with mean activity ratios of 1.02 and 0.89 for the high and low ratio period, respectively. The activity ratios in individual precipitation events allowed determinations of the mean irradiation period based on a simple non-steady-state model. The deduced irradiation periods averaged 27 days in the July-November 1991 period and 50 days in the combined March-June 1991 and January-March 1992 period, assuming a well-mixed trophosphere, efficient scavenging of the aerosols and a activity ratio of 0.7 for the ratio of the tropospheric production rates. The annual average irradiation time was estimated at 40 days, consistent with the calculation of the aerosol residence time in the upper troposphere. Furthermore, the tropospheric production rates of 32 P and 33 P were determined from the ratios, wet deposition rates, and aerosol residence time, and were consistent within the range of previously published estimates.

Waser, N. A. D.; Bacon, M. P.

1995-06-01

253

Monitoring of the 14C activity in the atmosphere  

International Nuclear Information System (INIS)

Complete text of publication follows. The distribution of atmospheric radiocarbon has been extensively investigated both in the Northern and Southern Hemisphere as well as in the Tropics for a number of decades. Systematic global observations of 14CO2 in the troposphere were made during and after atmospheric nuclear weapon tests in the 1950s and 1960s by several laboratories. Nowadays the monitoring of 14C in regions adjacent to nuclear power plants (NPP) or sites of land-filled radioactive wastes has growing importance in determining the frequency and activity of anthropogenic 14C released to the environment. On the other hand, the depletion of 14C in the atmosphere gives information about the regional fossil fuel CO2 contributions (Figure 1., Prague-Bulovka). The 14C activity of the atmosphere has been monitored in the vicinity of Paks NPP by sampling environmental air monthly since 1994. Four differential sampling units collect air samples less than 2 km away from the 100- m-high stacks of Paks NPP (A-type stations), and for reference a sampler is operated at a station (B24) ca. 30 km away from Paks NPP. The highest 14C values were measured at the site located less than 1km away from Paks NPP. The influence of the 14C discharge in the environment decreases rapidly with the distance from the source and under normal operating conditions the effect of Paks NPP is negligible at a distance of 2.5km. In Figure 1. we have compared our data for Paks NPP measured during the time span of 2000 - 2005 with data from different European monitoring stations. (The 14C activities are given in ? values: ?14C(% (Asample/Astandard-1) x 1000.) In 2000-2001 the excess 14C at Paks NPP compared to the B24 was 5-10 (per mille) but its ? value didn't exceed the tropospheric background (1). With the growing traffic the inactive CO2 emission (Suess effect) exceeded the influence of the NPP (2). After a cleaning tank incident at unit 2 of Paks NPP in April 2003 a 5-10 (per mille) rise of ?14CO2 was observed at all stations (3). During the remediation activities this decreased slowly in 2004 and ceased by the second half of 2005 (4). (author)

2003-04-00

254

Structure of newly synthesized (/sup 35/S)-proteoglycans and (/sup 35/S)-proteoglycan turnover products of cartilage explant cultures from dogs with experimental osteoarthritis  

Energy Technology Data Exchange (ETDEWEB)

The structure of newly synthesized proteoglycans from explant cultures of cartilage from joints subjected to transection of the anterior cruciate ligament (osteoarthritic) and from normal (non- or sham-operated) joints was examined. The structure of the products of proteoglycan turnover was also examined using explants of normal and osteoarthritic cartilage maintained in culture for a 48 h chase period. The findings were as follows: Newly synthesized (/sup 35/S)-proteoglycans extracted from cartilage explants from osteoarthritic joints whether examined 3 weeks, 3 months, or 6 months after surgery were larger than those from corresponding normal cartilage. This can be explained by the synthesis in osteoarthritic cartilage of abnormally long chondroitin sulfate chains on newly synthesised proteoglycans. The extracts also contained a newly formed small proteoglycan species that was unable to interact with hyaluronic acid. The proportion of this species was higher in osteoarthritic cartilage compared with normal, examined 3 weeks after surgery, but was generally absent from cartilage obtained 3 and 6 months after surgery. Compared with controls, a smaller proportion of the (/sup 35/S)-proteoglycans released into the maintenance medium of explant cultures of osteoarthritic cartilage during a 48 h chase period was able to interact with hyaluronic acid. However, although furnished with longer (/sup 35/S)-glycosaminoglycan chains, these proteoglycans were smaller than those from control explants.

Carney, S.L.; Billingham, M.E.; Muir, H.; Sandy, J.D.

1985-01-01

255

In vitro synthesis and purification of UDP-[14C] galacturonate  

International Nuclear Information System (INIS)

Pectins comprise a major component of the cell wall and much research has focused on degradation of pectins during ripening and senescence. However, little research has been conducted on pectin synthesis, partly due to a lack of commercial availability of UDP-[14C]galacturonic acid for use as a substrate in assaying galacturonan synthase. We report on the modification and integration of several procedures to synthesize UDP-[14C] galacturonic acid from commercially available UDP-[14C]glucuronic acid. A microsomal pellet containing UDP-D-glucuronate-4-epimerase was extracted from 5-day-old mung bean hypocotyls (Phaseolus aureus) and radish roots (Raphanus sativus L.) by ultracentrifugation at 30,000 rpm for 50 min. The UDP-[14C]galacturonic acid produced was separated from remaining UDP-[14C]glucuronic acid and other products by electrophoresis in pyridine:acetate:H2O on silica gel. Spots were detected by autoradiography, eluted with 80% ethanol, and purified using anion exchange chromatography

1989-01-01

256

Pharmacokinetics of 14C-urea in mice  

International Nuclear Information System (INIS)

Objective: To study the pharmacokinetics of 14C-urea in mice. Methods: Mice received one dose of 14C-urea orally. 14C concentrations in blood, organs and excrements of mice were measured by a Liquid Scintillation Counter. Results: Mice received one dose of 2.96 MBq/kg of 14C-urea orally, the blood concentration-time curve conformed to two compartment open model, T1/2(?) was 0.72 h, T1/2(?) was 5.15 h, Tmax was 0.11 h, Cmax was 1.57 kBq/ml, plasma clearance was 0.617 L?kg-1?h-1, respectively. Measurements conducted at 0.25, 2, 24 h after deglutition, respectively. In the 12 organs measured, radioactivity was found most intensive in kidney, liver, lung and spleen, less in heart, stomach, intestines and muscles, and least in brain and fat tissue. The peak was found at 2 h in lung. Radioactivity in the 12 organs measured reduced by time in 24 h but relatively higher concentration standing in liver. In 24 h, excretion via urine was 45.45%, stool excretion 19.30%, and that was 64.75% of intake dosage in total. Conclusion: Orally administrated 14C-urea will be quickly absorbed and cleaned out, mainly via urine, no specific accumulation in 12 organs was found.

2004-01-01

257

Calculation of the compounded uncertainty of 14C AMS measurements  

International Nuclear Information System (INIS)

[en] The correct method to calculate conventional 14C ages from the carbon isotopic ratios was summarised 35 years ago by Stuiver and Polach (1977) and is now accepted as the only method to calculate 14C ages. There is, however, no consensus regarding the treatment of AMS data, mainly of the uncertainty of the final result. The estimation and treatment of machine background, process blank, and/or in situ contamination is not uniform between laboratories, leading to differences in 14C results, mainly for older ages. As Donahue (1987) and Currie (1994), among others, mentioned, some laboratories find it important to use the scatter of several measurements as uncertainty while others prefer to use Poisson statistics. The contribution of the scatter of the standards, machine background, process blank, and in situ contamination to the uncertainty of the final 14C result is also treated in different ways. In the early years of AMS, several laboratories found it important to describe their calculation process in details. In recent years, this practise has declined. We present an overview of the calculation process for 14C AMS measurements looking at calculation practises published from the beginning of AMS until present.

2013-01-01

258

Whole-body autoradiographic study on the distribution of pivampicillin-14C labeled at the ampicillin and ester moieties in mice, as compared with those of ampicillin-14C, formaldehyde-14C and pivalic acid-14C  

International Nuclear Information System (INIS)

[en] The distribution of pivampicillin-14C labeled at the phenylglycyl and the oxymethylene moieties and ampicillin-14C were investigated by whole-body autoradiography after oral administration to mice. The distribution of formaldehyde-14C and pivalic acid-14C was also investigated for comparison. The results revealed that, i) pivampicillin is absorbed from intestine much more rapidly and to a larger extent than ampicillin, ii) the distribution pattern was, however, the same for the two drugs, being mainly restricted to the excretory organs such as the liver, kidney, gall and urinary bladders and intestinal tract, and iii) the oxymethylene moiety, the ester group, showed a completely different distribution pattern, being distributed in the bone marrow, pancreas, intestinal mucosa, spleen and thymus; the pattern being the same as that after administration of formaldehyde-14C. It was therefore suggested that pivampicillin is hydrolyzed in vivo to form ampicillin and formaldehyde before entering into the general circulation and that formaldehyde formed is partly incorporated into the general metabolic pathways in the tissues of a rapid cell division or protein synthesis. Pivalic acid, which is to be liverated simultaneously, was shown to be well absorbed, distributed in the bone, kidney, olfactory bulb, salivary gland and some adipose tissues and finally excreted into the urine mostly as its conjugated forms. (author)

1979-01-01

259

Validation and verification of the ICRP biokinetic model of 32P: the criticality accident at Tokai-Mura, Japan.  

UK PubMed Central (United Kingdom)

Regrettably, a criticality accident occurred at a uranium conversion facility in Tokai-mura, Ibaraki, Japan, on 30 September 1999. Radioactivities of 32P in urine, blood and bone samples of the victims, who were severely exposed to neutrons, were measured. 32P was induced in their whole bodies at the moment of the first nuclear release by the reaction 31P (n, gamma) 32P and 32S (n, p) 32P. A realistic biokinetic model was assumed, as the exchange of 32P between the extracellular fluid compartment and the soft tissue compartment occurs only through the intracellular compartment, and the model was used for preliminary calculations. Some acute excretion of 32P, caused by decomposition or elution of tissues which occurred at the time of the accident, may have happened in the victims' bodies in the first few days. The working hypotheses in the present work should initiate renewed discussion of 32P biokinetics.

Miyamoto K; Takeda H; Nishimura Y; Yukawa M; Watanabe Y; Ishigure N; Kouno F; Kuroda N; Akashi M

2003-01-01

260

Validation and verification of the ICRP biokinetic model of 32P: the criticality accident at Tokai-Mura, Japan.  

Science.gov (United States)

Regrettably, a criticality accident occurred at a uranium conversion facility in Tokai-mura, Ibaraki, Japan, on 30 September 1999. Radioactivities of 32P in urine, blood and bone samples of the victims, who were severely exposed to neutrons, were measured. 32P was induced in their whole bodies at the moment of the first nuclear release by the reaction 31P (n, gamma) 32P and 32S (n, p) 32P. A realistic biokinetic model was assumed, as the exchange of 32P between the extracellular fluid compartment and the soft tissue compartment occurs only through the intracellular compartment, and the model was used for preliminary calculations. Some acute excretion of 32P, caused by decomposition or elution of tissues which occurred at the time of the accident, may have happened in the victims' bodies in the first few days. The working hypotheses in the present work should initiate renewed discussion of 32P biokinetics. PMID:14526956

Miyamoto, K; Takeda, H; Nishimura, Y; Yukawa, M; Watanabe, Y; Ishigure, N; Kouno, F; Kuroda, N; Akashi, M

2003-01-01

 
 
 
 
261

Validation and verification of the ICRP biokinetic model of 32P: The criticality accident in Tokai-mura, Japan  

International Nuclear Information System (INIS)

Regrettably, a criticality accident occurred at a uranium conversion facility in Tokai-mura, Ibaraki, Japan, on 30 September, 1999. Radioactivities of 32P in urine, blood and bone samples of the victims who were severely exposed to neutrons were measured. 32P was induced in their whole bodies at the moment of the first nuclear release by the reaction 31P(n,gamma)32P and 32S(n,p)32P. A realistic biokinetic model was assumed as the exchange of 32P between the extracellular fluid compartment and soft tissue compartment occurs only through the intracellular compartment, and the model was used for preliminary calculations. Some acute excretion of 32P, caused by decomposition or elution of tissues which occurred at the time of the accident, may have happened in the victims' bodies in the first few days. The working hypotheses in the present work should initiate renewed discussion of 32P biokinetics. (author)

2003-01-01

262

Method of preparing (U-14C)mannose-6-phosphate  

International Nuclear Information System (INIS)

The initial compound for the preparation of (U-14C)mannose-6-phosphate is a water-soluble polysaccharide (e.g., branched polysaccharide of the 1?4,6 glycan type) separated from radioactive biological material (e.g., from green or blue-green algae). The polysaccharide is gradually reacted to a number of enzymes to obtain glucose-6-phosphate (U-14C). This intermediate product is exposed to the action of the mannosephosphate isomerase enzyme in a buffered medium of pH of 7.2. The resulting mannose-6-phosphate(U-14C) is separated with paper chromatography. The reaction yield is up to 38%. The advantage of the said method of preparation is that it involves directly the water-soluble polysaccharide obtained within the complex treatment of radioactive biomass. (E.S.).

1983-07-11

263

Preparation of poly methyl methacrylate labeled with 14C  

International Nuclear Information System (INIS)

14C labeled poly methyl methacrylate can be used as a radioactive source for measuring the thickness of paper in the production lines. Very thin and uniform sources can be obtained by the method. Its preparation procedures are as follows: (1)Synthesis of Argerfine methacrylicate. (2)Preparation of poly methyl methacrylate labelled with 14C. Argerfine methacrylicate is mixed and refluxed with 14C labeled iodine methane, at 72 degree C for five hours in a bottle. (3)The preparation of the radioactive source. Initiating agent is added into the bottle. The bottle is heated to 75 degree C for one hour, then heated to 100 degree C for another one hour. The reactive product is sealed with epoxy resin. (authors)

2004-01-01

264

The absorbed dose to bone marrow in the treatment of polycythaemia by 32P  

International Nuclear Information System (INIS)

Measurements have been made of absorbed dose to bone marrow in the treatment of polycythaemia by 32P, based on the determination of activities in bone and marrow biopsies taken at various times from 1 to 27 days after injection of the radionuclide. Activities were measured in the cortex, trabeculation and marrow of biopsies taken from the iliac crest, and also in sternal marrow. The biological half-life of 32P in marrow from the iliac crest was found to be nine days; that derived for sternal marrow was lower, but the difference was not statistically significant; the value for trabecular bone was 27 days. The biological half life for 32P in the body, as measured by whole-body counting, was 39 days. Calculations of the dose-rate to trabecular marrow have been made by a method based on that of Whitwell and Spiers (1971, Proceedings of the Fifth Congress of the French Society for Radioprotection, Grenoble, France, 401), but modified to allow for the presence of 32P in the marrow as well as in trabecular bone. The dose-rates followed a single exponential decay with a half-life of 6.7 days. The integrated dose including that during the first day was 24 rad per mCi injected. (author)

1976-01-01

265

The absorbed dose to bone marrow in the treatment of polycythaemia by 32P.  

UK PubMed Central (United Kingdom)

This paper reports the determination of absorbed dose to bone marrow in the treatment of polycythaemia by 32P, based on the measurement of activities in bone and marrow biopsies taken at various times from 1 to 27 days after injection of the radionuclide. Activities were measured in the cortex, trabeculation and marrow of biopsies taken from the iliac crest, and slso in sternal marrow. The biological half-life of 32P in marrow from the iliac crest was found to be nine days; that derived for sternal marrow was lower, but the difference was not statistically significant; the value for trabecular bone was 27 days. The biological half life for 32P in the body, as measured by whole-body counting, was 39 days. Calculations of the dose-rate to trabecular marrow have been made by a method based on that of Whitwell and Spiers (1971), but modified to allow for the presence of32P in the marrow as well as in trabecular bone. The dose-rates follow a single exponetial decay with a half-life of 6.7 days. The intergrated dose including that during the first day is 24 rad per mCi injected.

Spiers FW; Beddoe AH; King SD

1976-02-01

266

Calibration of TLD cards to beta ray spectra of 32P  

International Nuclear Information System (INIS)

[en] The results of the evaluation of TLD chips are received in nC, and we need to transform these values to mGy, in order to obtain the penetrating and the non-penetrating dose. The calibration factors were determined experimentally by irradiation the TLD chips with an uranium source. Beta rays having other spectra can cause incorrect values when estimating the non-penetrating dose. If the spectrum of the beta source is known, a specific evaluation of the calibration factor can be performed. In this report, the estimation of the calibration factor for a 32P source is presented. LiF:Ti,Mg TLD cards were irradiated calibrated source of 90Sr/90Y and 204Tl and non-calibrated source of 32P, in order to find the beta correction factor for the spectrum of 32P. Calculations of the beta correction factor were performed too, by applying the Loevinger equations to the geometry of the TLD chips used in our routine measurements. The calculated values of the beta correction factors are lower than the experimental ones. When comparing the ratios, between the beta factors of 32P and 90Sr/90Y received from the experiments and from the calculation, we found them to be constant up to ±5%. (authors) 15 refs, 2 figs, 6 tabs

1994-01-01

267

Uptake of 3HHO and 32P by roots of wheat and rape  

International Nuclear Information System (INIS)

Direct measurements were made of 3HHO and 32P taken up from labelled soil by roots of wheat (Triticum aestivum L.) and rape (Brassica campestris L.). Single roots were encased in labelled soil for 3 days, and the amount of 3HHO and 32P retained in the shoots was determined. Plants were grown to five stages of maturity in growth boxes under controlled conditions. Roots were labelled at up to four depths (to 90 cm) depending on the rooting depth at each stage of maturity. Uptake of 3HHP per unit length of root increased as the plant age increased, while uptake of 32P decreased to below detection levels by 45 days after germination. Larger amounts of both nutrients were translocated to and retained in the shoots from surface roots than from roots located deeper in the soil although the soil was uniform in temperature, bulk density, and composition through the growth boxes. Wheat roots were more efficient than rape roots in absorbing 3HHO; however, rape roots took up larger amounts of 32P per unit length of root. Neither native nor added P located more than 30 cm deep is of much importance to these annual crops, since uptake is minimal and the main demand for this nutrient occurs at early growth stages when the root system is restricted to the surface layers

1977-01-01

268

{sup 14}C-AMS in biomedical research  

Energy Technology Data Exchange (ETDEWEB)

Accelerator mass spectrometry has been applied to biomedical research in the past 5 years. While there are a number of facilities involved in biomedical measurements of {sup 26}Al and {sup 41}Ca, the use of {sup 14}C-AMS for tracing organic compounds in vivo and in vitro remains somewhat isolated at our laboratory with a few contributions from two other AMS systems. We present some examples of our research using {sup 14}C-AMS. Our successes in measuring low dose kinetics of chemical compounds has implications for pharmacology, toxicology, carcinogenisis, nutrition, drug development and clinical tests.

Vogel, J.S.; Turteltaub, K.W.; Mauthe, R.; McAninch, J.E. [Lawrence Livermore National Lab., CA (United States)] [and others

1995-12-01

269

AMS of {sup 14}C at low energies  

Energy Technology Data Exchange (ETDEWEB)

At the ETH/PSI AMS facility {sup 14}C test measurements have been performed at low terminal voltages of 0.5 and 1 MV in order to study the feasibility of AMS with very small accelerators. These experiments have demonstrated that interfering molecules ({sup 13}CH and {sup 12}CH{sub 2}) in charge states 1{sup +} and 2{sup +} can be destroyed in an adequate Ar gas stripper. These new results imply the feasibility of a new generation of very small accelerator systems for {sup 14}C. (author) 1 fig., 3 refs.

Suter, M.; Huber, R.; Jacob, S. [ETHZ, Zurich (Switzerland); Synal, H.A. [Paul Scherrer Inst. (PSI), Villigen (Switzerland)

1997-09-01

270

Synthesis of D-(1-/sup 14/C)penicillamine hydrochloride  

Energy Technology Data Exchange (ETDEWEB)

Methods are described for the synthesis of DL-(1-/sup 14/C)penicillamine and D-(1-/sup 14/C)penicillamine hydrochloride from K/sup 14/CN. The method consists of addition of H/sup 14/CN to 2,2,5,5-tetramethyl-3-thiazoline followed by hydrolysis of the resulting thiazolidine to DL-penicillamine. Resolution was achieved through the salt of N-formylisopropylidene-DL-penicillamine with (-)-norephedrine. The specific activity of D-penicillamine was 21.7 mCi/mmole and the overall radiochemical yield from K/sup 14/CN was 3.3%. (author).

Chien, D.H.T.; Ebert, D.A.; Roth, R.W.

1988-11-01

271

Spontaneous emission of 14C from 223Ra  

International Nuclear Information System (INIS)

Rose and Jones recently discovered in an ingeniously simple experiment that 223Ra, an ? emitter with a half-life of 11.4 days, occasionally emits 14C nuclei of 29.8 MeV energy. The branching ratio relative to ? decay was measured to be (8.5 +/- 2.5) x 10-10. 223Ra occurs in the natural decay chain of 235U, with 227Ac being a convenient precursor and source material. In the present study the authors have confirmed the spontaneous emission of 14C from 223Ra and unambiguously identified the mass of the emitted particles

1984-04-01

272

Synthesis of /sup 14/C-labeled stepholidine  

Energy Technology Data Exchange (ETDEWEB)

L-Tetrahydroprotoberberine (THPB) alkaloids are dopamine-receptor antagonists. Stepholidine has been shown to possess the strongest pharmacological effects among the THPB alkaloids studied. In order to study its metabolism and the mode of action of the drug, a radiolabeled stepholidine was required. We report here the synthesis of /sup 14/C-labeled stepholidine by Mannich condensation of 7-benzyloxy-1-(4-benzyloxy-3-hydroxy-benzyl)-6-methoxy-1,2,3,4-tetrahydroisoquinoline with (/sup 14/C)formaldehyde followed by methylation and debensylation in 32% radiochemical yield.

Yang Liu; Zhang Xin

1988-05-01

273

Method of preparing D-maltose(U-14C)  

International Nuclear Information System (INIS)

[en] 14C-labelled D-maltose is prepared from a polysaccharide soluble in water, separated from green and/or blue-green algae in the course of the complex processing of the radioactive biomass. A buffered solution of the said polysaccharide is hydrolyzed with beta amylase. The maltose obtained is separated from the unconverted dextrin using paper chromatography. The radiochemical yield is about 35%. The method allows preparing 14C-labelled D-maltose with an activity of about 100 mCi/millimole. (Ha)

1981-01-01

274

Causes of an AD 774-775 14C increase  

CERN Document Server

Atmospheric 14C production is a potential window into the energy of solar proton and other cosmic ray events. It was previously concluded that results from AD 774-775 are orders of magnitude greater than known solar events. We find that the coronal mass ejection energy based on 14C production is much smaller than claimed, but still substantially larger than the maximum historical Carrington Event of 1859. Such an event would cause great damage to modern technology, and in view of recent confirmation of superflares on solar-type stars, this issue merits attention.

Melott, Adrian L; 10.1038/nature11695

2012-01-01

275

The glyceryl [14C] tripalmitate breath test: A reassessment  

International Nuclear Information System (INIS)

Several reports have been published commending the use of 14C-labelled triglyceride breath tests in the assessment of fat malabsorption. The authors report further studies using glyceryl [14C]tripalmitate. Corrections for age, weight or metabolic rate failed to improve the test's ability to discriminate between malabsorbers and control subjects. A correction for respiratory quotient improved the linear correlation observed between the breath test results and daily faecal fat excretion. The significance of these findings is discussed and a number of problems identified which, at present, are preventing the introduction of breath tests for fat malabsorption into routine clinical practice. (Auth.).

1980-01-01

276

Incorporation of (2-14C) acetate and (1-14C) palmitate into buffalo sperm lipids  

International Nuclear Information System (INIS)

[en] The incorporation of (2-14C)acetate and (1-14C)palmitate into the lipids of washed ejaculated buffalo spermatozoa was followed up to 4 and 2 hr respectively. There was significant incorporation of acetate into the total lipids both in the presence and absence of glucose in the medium. The presence of glucose (0.5%) in the medium significantly stimulated the incorporation of acetate into the sperm lipids at 2 hr. Inclusion of 3% bovine serum albumin in the incubation medium significantly stimulated the acetate incorporation at 4 hr whereas no such effect was observed at 2 hr of incubation. The incorporation of (1-14C)palmitate into buffalo sperm lipids was significantly high in the presence of glucose and was highest at 1 hr of incubation. No incorporation of (1-14C)palmitate was observed in the absence of glucose. The greater extent of palmitate incorporation than acetate suggested that the de novo synthesis of lipids using 14C-acetate was comparatively lower than utilization of 14-palmitate for esterification with sperm lipids in buffaloes. (author)

1981-01-01

277

Effect of tolbutamide on 14C-sodium bicarbonate and 14C-alanine metabolism in isolated rat hepatocytes  

International Nuclear Information System (INIS)

Tolbutamide (TOLB) is a sulfonylurea commonly used in the treatment of noninsulin-dependent diabetes mellitus. Studies have shown that TOLB affects gluconeogenesis and glycolysis from various substrates in the liver. Specifically, TOLB inhibits gluconeogenesis from lactate in a dose-dependent manner. In order to further clarify tolbutamide's mechanism of action, its effect on the incorporation of 14C from NaH14CO3 and 14C-alanine into glucose, lactate or pyruvate in the presence of lactate was measured. Rat hepatocytes were incubated with lactate (2.0 mM) with or without TOLB (1.0 mM) in the presence of NaH14CO3 or 14C-alanine. TOLB inhibited the incorporation of C14 from NaHCO3 and alanine into glucose by 55 and 56%, respectively. TOLB did not alter the incorporation of C14 from NaHCO3 into lactate or pyruvate. TOLB did not affect the incorporation of 14C from alanine into lactate but produced a pooling of 14C as pyruvate. The authors data support studies demonstrating the TOLB produces its actions, in part, by increasing the concentration of fructose-2,6-bisphosphate and inhibiting pyruvate carboxylase

1990-02-26

278

Use of colloids of chromic [{sup 32}P] phosphate in treatment of solid tumors  

Energy Technology Data Exchange (ETDEWEB)

In order to evaluate the effectiveness of an intratumorally single dose of chromic [{sup 32}P] phosphate for the treatment of solid tumors, studies of bioelimination, biodistribution, and therapeutic action were carried out. Only for comparative purposes were similar studies undertaken using a solution of sodium [{sup 32}P] orthophosphate-gelatin. Results show that when sodium [{sup 32}P] orthophosphate-gelatin was intratumorally injected, the percentage of total elimination, after 32 days of treatment, was equal to 85.90 {+-} 8.70%, with a higher percentage in urine (64.50 {+-} 13.70%) than in feces (21.40 {+-} 4.50%). In biodistribution studies, the greater percentage was found in bone (15.54 {+-} 2.21%), whereas only 2.51 {+-} 0.39% remained in the tumor. When chromic [{sup 32}P] phosphate was intratumorally injected, we found that the total elimination was equal to 51.70 {+-} 6.90%, with a higher amount in feces (32.70 {+-} 4.80%) than in urine (19.00 {+-} 3.60%). Biodistribution studies demonstrated that 28.93 {+-} 1.30% was still in the tumor and 19.01 {+-} 1.30% of the injected activity was found in the liver. On the other hand, when therapeutic action was evaluated, no tumoral regression was observed. These results demonstrate that the colloid of chromic [{sup 32}P] phosphate cannot be used in the treatment of solid tumors as it mobilizes from the injection point, delivering a high dose to the entire organism.

Zubillaga, M.B.; Boccio, J.R.; Nicolini, J.O.; Ughetti, R.; Lanari, E

1996-10-01

279

Synthesis of organic compounds with disulfide bridge labelled with 35 S  

International Nuclear Information System (INIS)

This work had as purpose: a) synthesis of aliphatic compounds with disulfide bridge, and also the synthesis of aromatic compounds with disulfide bridge, substituted with various functional groups; b) labelling with 35 S of certain positions of the obtained organic compounds having disulfide bridge in their molecular structures. At the same time, we studied the 35 S-labelling techniques of these compounds. The organic syntheses used in the process of labelling of organic compounds containing disulfide bridge, have the advantage to obtain a structure with a definite position of radioactive labelling (of 35 S). The 35 S radioisotope is an important element due to its applications in the field of organic compounds labelling. Among its characteristics we mention: the half time is 87 days, the maximum energy of beta radiation is 0.167 MeV and it constitutes a pure beta transmitter. The activity measurements were carried out with a 3385 Tricarb-Packard spectrometer, based on liquid scintillators. Thanks to its properties 35 S has several applications in organic chemistry and biology. (authors)

1999-01-01

280

(/sup 35/S) Proteoglycan metabolism of arterial smooth muscle cells cultured from normotensive and hypertensive rats  

Energy Technology Data Exchange (ETDEWEB)

Arterial smooth muscle cells cultured from normotensive and hypertensive rats incorporated (/sup 35/S)sulfate into the extracellular and pericellular sulfated proteoglycans and endocytose extracellular (/sup 35/S)proteoglycans at a significantly higher rate in the phase of logarithmic growth than did nondividing cells. /sup 35/S incorporation into proteoglycans was positively correlated with (/sup 3/H)thymidine incorporation into cellular TCA-precipitable material. The rates of (/sup 35/S)proteoglycan synthesis and endocytosis per cell per day were higher in smooth muscle cells from hypertensive than from normotensive animals, the observed differences being related to a higher average protein content of smooth muscle cells cultured from hypertensive rats as compared with cells of normotensive animals. Gel filtration under dissociative conditions separated the (/sup 35/S)proteoglycans into high and low molecular weight fractions (A,B) differing in glycosaminoglycan composition and their ability to be endocytosed by smooth muscle cells. The relative proportion of the high molecular weight proteoglycan fraction A decreased continuously from sparse to confluent cell cultures.

Schmidt, A.; Grounwald, J.; Buddecke, E. (Muenster. Univ. (Germany, F.R.). Inst. of Atherosclerosis Research and Inst. of Physiological Chemistry)

1982-12-01

 
 
 
 
281

Cerenkov and liquid scintillaton analysis of the triple label 86Rb-35S-33P  

International Nuclear Information System (INIS)

A method was developed for the analysis of 86Rb-35S-33P as a triple-label. The analysis of the total activity of composite mixtures of the three radionuclides was demonstrated to be quantitative by the liquid scintillation efficiency tracing technique with recoveries between 99.7-100.4%. The activity of 86Rb in the triple label was determined by Cerenkov counting in water with a 52% counting efficiency. The combined activities of 35S + 35P were determined by subtracting the activity of 86Rb from the total activity of the triple label. The separate activities of 35S and 33P are obtained by difference after a period of time sufficient for the decay of 33P to negligible levels leaving only residual 35S in the sample. The method described is useful for the analysis of 86Rb-35S-33P as a triple label for the elements K-S-P in studies of fertilizer use efficiency. (Author).

1996-01-01

282

Synthesis of 14C labelled lidocaine (?-diethylamino acet-2,6-dimethylanilide)  

International Nuclear Information System (INIS)

14C-lidocaine was composed by 14C-diethylamine reaction with ?-chloroacetic-2,6-dimethylaniline. 14C-diethylamine was prepared from Ba14CO3 via K14CN and 14C-acetonitrile which was hydrogenated. Radiochemical purity of 14C-diethylamine and 14C-lidocaine is >99% by HPLC and TLC respectively. 14C-lidocaine is needed for breath assay of mouse for measuring liver function

2002-01-01

283

Incorporation of U-14C-glucose into the lipids U-14C and sugars of Culex pipiens fatigans  

International Nuclear Information System (INIS)

The role of U-14C-glucose as a lipogenic precursor was examined in larvae and adults of C. pipiens fatigans. The pattern of incorporation of the radiocarbon was also examined in the sugars of these insects. It was found that on feeding of larvae and adults with 14C-glucose, the phospholipid fraction of the insects was associated with more than 70 per cent of the total lipid radiolabel. However, the percentage distribution of the phospholipid label in the different phospholipid fractions of the larvae and adults was different. Among the neutral lipids, a low but significant label in cholesterol was of considerable interest. The 14C-glucose was also converted to trehalose in both larvae and adults. However, the two stages differed in the utilization of glucose and trehalose. (author).

1983-01-01

284

Uptake of Glucose-1-14C by Pullularia pullulans  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Pullularia pullulans cells were grown in a medium containing yeast extract, malt extract, glucose, and nutrient salts, in addition to glucose-1-14C. The lipids extracted from the cells were fractionated by use of a single column packed with silicic acid. Of the total radioactive carbon added to the ...

Merdinger, Emanuel

285

Assessment of the 14C-Glycocholic Acid Breath Test  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The 1-(14C)-glycine-glycocholic-acid breath test has been performed on 104 subjects and a normal range established. Abnormal results due to bacterial deconjugation of bile salts were found not only in patients with the “contaminated bowel” syndrome and in those with ileal resection but also in a thi...

James, O. F. W.; Agnew, J. E.; Bouchier, I. A. D.

286

Process for the production of 14C-labelled compounds  

International Nuclear Information System (INIS)

The patent describes the production of 14C-labelled compounds from solution with the aid of algae. A microorganism of the Anacystic species is used, preferably Anacystis nidulans which is also known as 'Indiana 625'. The experiments and their results are described in detail. (UWI).

1978-01-01

287

Method of preparing (U-14C)-D-galactose  

International Nuclear Information System (INIS)

Whole Chlorella algae cells cultivated in a 14CO2 medium were subjected to a ?-galactosidase solution buffered to pH=4.5 to 6.0. The produced (U-14C)-D-galactose is separated by paper chromatography. (E.S.).

1980-02-29

288

Applications of AMS 14C on Climate and Archaeology  

International Nuclear Information System (INIS)

We describe the Accelerator Mass Spectrometry (AMS) technique and two distinct applications of its use with 14C to study environmental problems in Brazil, such as forest fires and climate changes in the Amazon region and archaeological studies on the early settlements in the Southeast Brazilian coast

2007-10-26

289

Radial transport of 14C in Norwegian pine  

International Nuclear Information System (INIS)

[en] With the purpose to contribute to a dendro-calibration curve for 14C dating we turned our attention to pine trees (Pinus sylvestris) in a high mountain area (62degN) of Norway. This area seemed to be sufficiently homogeneous to allow the build-up of a 400 years master chronology based on living trees. There should also be possibilities of finding old logs in the ground in order to extend the chronology further back. As concerns the 14C measurements, radial transport of bomb-produced 14C was reflected in the heartwood early in this century. A reflected peak in AD 1930 constituted a 14C activity of 6.7 +- 0.5% above normal level in untreated wood and was of advantage for testing the purification procedure. A complete removal of the contamination from outer rings became possible with inorganic solvents (KOH and NaClO2), where only the cellulose was extracted for measurement. The delta13C values are influenced by the pretreatment in accordance with the degree to which the various fractions are removed. Differences in delta13C values due to various pretreatment procedures may constitute up to 4 per thousand. (author)

1981-08-19

290

Bound residues of 14C-chlorotoluron in soil  

International Nuclear Information System (INIS)

Dynamics of bound residues of 14C-chlorotoluron, distribution of the bound residues in the components of humus, and their absorption by step-crop (rice) were investigated in paddy soil and black soil under simulated aerobic and anaerobic conditions respectively. The results show that: (1) The bound residues of 14C-chlorotoluron in soil were very considerable and increasing with the incubation time. The organic substance and humidity contained in the soil affected the bound residues remarkably. The bound residues in black soil with more organic substance and clay particles were higher than those in paddy soil, and under anaerobic conditions were higher than under aerobic conditions. (2) Bound residues of 14C-chlorotoluron mainly bound with the components of soil humus, the distribution of which was in the order of humin>humic acid>fulvic acid. (3) Bound residues of 14C-chlorotoluron in soils could be absorbed by step-crop (rice) and moved to the upper part, that would cause chemical injury, chlorosis and necrosis for the leaf tip.

1992-01-01

291

Pharmacokinetics and metabolism of [14C]rosaramicin in dogs.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The pharmacokinetics and metabolism of [14C]rosaramicin were studied in dogs after intravenous (i.v.; 10 mg/kg [bodyweight]) and oral (25 mg/kg) administration. After i.v. administration, rosaramicin levels in plasma declined rapidly, with half-lives of 0.22 h for the distribution phase and 0.97 h f...

Lin, C C; Kim, H K; Marco, A; Radwanski, E; Symchowicz, S

292

Photosynthetic incorporation of 14C by Stevia rebaudiana  

International Nuclear Information System (INIS)

The photosynthetic incorporation of 14 by Stevia rebaudiana specimens was investigated. The 14C incorporation, when the isotope was furnished to the plant in form of 14CO2, was rapid. After 24 hours, the radioactivity has been incorporated into a great number of compounds including pigments, terpenes, glucose, cellulose and also stevioside and its derivatives. (M.A.C.)

1985-01-01

293

Microorganisms labelled with /sup 14/C for measurement of phagocytosis  

Energy Technology Data Exchange (ETDEWEB)

An isotopic method for determination of phagocytic activity is presented. The method of labelling of Staphylococcus aureus 519 with /sup 14/C is described. Effects of various parameters (e.g. time of incubation, serum dilution, number of phagocytes and bacteria used in the test on phagocytosis) were studied. The effect of tuftsin and its lysine analogue on phagocytic granulocytes is also described.

Steuden, W. (Polska Akademia Nauk, Wroclaw. Inst. Immunologii i Terapii Doswiadczalnej)

1980-01-01

294

Synthesis of (2' - /sup 14/C) 8-methoxypsoralen  

Energy Technology Data Exchange (ETDEWEB)

The synthesis, the purification as well as checks for purity and identity of (2'-/sup 14/C) 8-methoxy-psoralen are described. The over-all yields, preparatively as well as radioactively was 16% of the theoretical amount.

Zipp, H.; Zimmer, A.; Koss, F.W. (Thomae (K.) Chemisch-Pharmazeutische Fabrik G.m.b.H., Biberach an der Riss (Germany, F.R.). Abt. Biochemie)

1982-09-01

295

One pot synthesis of sodium 14C-formate  

International Nuclear Information System (INIS)

[en] A one pot synthesis of sodium 14C-formate by reduction of 14CO2 with NaBH(OMe)3 in THF solution is described. This process is suitable for large scale, high specific activity preparations. (author)

1986-01-01

296

Modified synthesis of 11-[{sup 14}C]-clozapine  

Energy Technology Data Exchange (ETDEWEB)

The reported synthetic pathway of 8-chloro-11-(4-methyl-1-piperazinyl)-11-[{sup 14}C]-5H-dibenzo[b,e][1,4]diazapine (clozapine) was modified in several steps. The synthetic pathway was shortened by 60% and the total yield was increased from 6% to 23%.

Matloubi, Hojatollah E-mail: hmatloubi@seai.neda.net.ir; Ghandi, Mehdi; Zarrindast, M.-R.; Saemian, Nader

2001-11-01

297

Modified synthesis of 11-[14C]-clozapine  

International Nuclear Information System (INIS)

The reported synthetic pathway of 8-chloro-11-(4-methyl-1-piperazinyl)-11-[14C]-5H-dibenzo[b,e][1,4]diazapine (clozapine) was modified in several steps. The synthetic pathway was shortened by 60% and the total yield was increased from 6% to 23%.

2001-01-01

298

Excretion of radioactivity following the intraperitoneal administration of /sup 14/C-DDT, /sup 14/C-DDD, /sup 14/C-DDE and /sup 14/C-DDMU to the rat and Japanese Quail  

Energy Technology Data Exchange (ETDEWEB)

A study in progress to examine the metabolic fate of DDT in birds and mammals is discussed. The first phase of the study, which is reported in this article, has been to establish the rate of excretion of ratioactivity following the intraperitoneal administrations of /sup 14/C-DDT, /sup 14/C-DDE, /sup 14/C-DDD, and /sup 14/C-DDMU to male rats and male Japanese quail. The mean values from the three animals in each experimental group for the amount of radioactivity excreted daily are given, and it was found that the rats excreted the radioactivity administered as DDT, DDD, and DDE substantially faster than did the quail. DDMU was excreted relatively rapidly and at similar rates. This finding suggests that apparent differences in the rates of excretion of DDT by birds and mammals probably arise from differences in the conversion of DDT to DDD or DDE or in the degradation of these metabolites to DDMU. The Japanese quail differ from the rats in excreting substantial amounts of unchanged DDT, DDE, and DDD, which probably reflects the inability of the Japanese quail to readily metabolise these compounds.

Fawcett, S.C. (Univ. of Surrey, Guildford, England); Bunyan, P.J.; Huson, L.W.; King, L.J.; Stanley, P.I.

1981-09-01

299

Studies on absorption, conversion of sulfate by rape seedling and accumulation of glucosinolate in rape seed by using 35S  

International Nuclear Information System (INIS)

The seedlings grown in sandy culture absorbed 35SO42- from cultural solution rapidly and the 35S was incorperated into glucosinolates, amino acids and proteins. Percentage of glucosinolates-35S in extractable 35S with 70% methanol declined and those of amino acid-35S rose regularly with time after labelling. The relative content of 35S incorperated into bound constituents as well as extractable components was constant after 5 days of labelling. From two weeks after flowering, the relative content of GS-35S in pods and seeds increased linearly with time until eight weeks after flowering when all 35S extracted with 70% methanol in seeds were in form of glucosinolate. The amount of 35S-GS per pod increased linearly from the first week to the eighth week after flowering. It seems that the accumulation of GS in seeds is related to the accumulation of dry matter in seeds.

1991-01-01

300

Convenient preparative synthesis of [14C]trehalose from [14C]glucose by intact Escherichia coli cells  

International Nuclear Information System (INIS)

[en] At high osmolarity, Escherichia coli synthesizes trehalose intracellularly, irrespective of the nature of the carbon source. Synthesis proceeds via the transfer of UDP-glucose to glucose 6-phosphate, yielding trehalose 6-phosphate, followed by its dephosphorylation to trehalose. This reaction was exploited to preparatively synthesize [14C]trehalose from exogenous [14C]glucose by using intact bacteria of a mutant (DF214) that could not metabolize glucose. The total yield of radiochemically pure trehalose from glucose was routinely more than 50%

1989-01-01

 
 
 
 
301

Convenient preparative synthesis of ( sup 14 C)trehalose from ( sup 14 C)glucose by intact Escherichia coli cells  

Energy Technology Data Exchange (ETDEWEB)

At high osmolarity, Escherichia coli synthesizes trehalose intracellularly, irrespective of the nature of the carbon source. Synthesis proceeds via the transfer of UDP-glucose to glucose 6-phosphate, yielding trehalose 6-phosphate, followed by its dephosphorylation to trehalose. This reaction was exploited to preparatively synthesize ({sup 14}C)trehalose from exogenous ({sup 14}C)glucose by using intact bacteria of a mutant (DF214) that could not metabolize glucose. The total yield of radiochemically pure trehalose from glucose was routinely more than 50%.

Brand, B.; Boos, W. (Univ. of Konstanz (Germany, F.R.))

1989-09-01

302

Characterization of excited states of 15N through 14C(p,p) 14C using polarized proton beam  

International Nuclear Information System (INIS)

Angular distributions of cross sections and analyzing powers were measured for 14C(p,p) 14C for proton energies between 3.7 and 11.0 MeV in very small energy steps. A number of strong resonances are seen. Phase-shift analysis of the elastic scattering data yielded level parameters of eleven states in 15N in the excitation energy region 13.7-21.0 MeV. Previous assignments of spin, parity and energy of levels are discussed. (Author)

2011-01-01

303

Sulfur mineralization of different plant materials labelled with {sup 35} S; Mineralizacao do enxofre de diferentes materiais vegetais marcados com {sup 35} S  

Energy Technology Data Exchange (ETDEWEB)

This study was carried out, in green house conditions, with the objective of evaluating the effect of incorporation in soil of different plant materials labelled with {sup 35} S and of incubation periods on the availability of sulfur to the bean test plants and on the dynamic of this element in the soil. The bean test plants dry matter yield ranged from 2.00 to 3.79 g/plant, the S content and absorption ranged from 118.20 to 194.04 mg/100 g and 2.61 to 6.34 mg/plant respectively. The {sup 35} S derived from the incorporated bean plant material contributed with 12 to 256% of total S absorbed by bean test plant; rice plant material contributed with 12 to 22%; soybean plant material contributed 11 to 18%; corn plant material at rate of 7 g with, 11 to 19% and corn at rate of 3.5 g; with 7 to 1%. Plant material incorporation showed {sup 35} S using efficiency by bean test plant of 21.41 to 9.94% by incorporated rice plant material, of 16.12 to 7.79% by rice material, of 13.11 to 6.49% by soybean material, of 10.24 to 6.21% by corn at rate of 3.5 g and of 7.41 to 3.81% by corn at rate of 7 g.Incorporated plant material with C/S relationship near 120, such as bean and rice, promoted desirable and favorable alteration in soil, while materials with C/S higher than 250, such as soybean and corn, led to unsatisfactory and undesirable alteration. The characteristic of incorporated plant materials which more affected its behavior was the C/S relationship, which depended on its physiological stage on collect timing. (author). 82 refs., 12 figs., 11 tabs.

Abreu Junior, Cassio H.

1993-01-01

304

Reconstructing Ocean Circulation using Coral (triangle)14C Time Series  

Energy Technology Data Exchange (ETDEWEB)

We utilize monthly {sup 14}C data derived from coral archives in conjunction with ocean circulation models to address two questions: (1) how does the shallow circulation of the tropical Pacific vary on seasonal to decadal time scales and (2) which dynamic processes determine the mean vertical structure of the equatorial Pacific thermocline. Our results directly impact the understanding of global climate events such as the El Nino-Southern Oscillation (ENSO). To study changes in ocean circulation and water mass distribution involved in the genesis and evolution of ENSO and decadal climate variability, it is necessary to have records of climate variables several decades in length. Continuous instrumental records are limited because technology for continuous monitoring of ocean currents (e.g. satellites and moored arrays) has only recently been available, and ships of opportunity archives such as COADS contain large spatial and temporal biases. In addition, temperature and salinity in surface waters are not conservative and thus can not be independently relied upon to trace water masses, reducing the utility of historical observations. Radiocarbon in sea water is a quasi-conservative water mass tracer and is incorporated into coral skeletal material, thus coral {sup 14}C records can be used to reconstruct changes in shallow circulation that would be difficult to characterize using instrumental data. High resolution {Delta}{sup 14}C timeseries such as ours, provide a powerful constraint on the rate of surface ocean mixing and hold great promise to augment one time oceanographic surveys. {Delta}{sup 14}C timeseries such as these, not only provide fundamental information about the shallow circulation of the Pacific, but can also be directly used as a benchmark for the next generation of high resolution ocean models used in prognosticating climate. The measurement of {Delta}{sup 14}C in biological archives such as tree rings and coral growth bands is a direct record of the invasion of fossil fuel CO{sub 2} and bomb {sup 14}C into the atmosphere and surface oceans. Therefore the {Delta}{sup 14}C data that are produced in this study can be used to validate the ocean uptake of fossil fuel CO2 in coupled ocean-atmosphere models. This study takes advantage of the quasi-conservative nature of {sup 14}C as a water mass tracer by using {Delta}{sup 14}C time series in corals to identify changes in the shallow circulation of the Pacific. Although the data itself provides fundamental information on surface water mass movement the true strength is a combined approach which is greater than the individual parts; the data helps uncover deficiencies in ocean circulation models and the model results place long {Delta}{sup 14}C time series in a dynamic framework which helps to identify those locations where additional observations are most needed.

Kashgarian, M; Guilderson, T P

2001-02-23

305

Design and construction of a prototype for the obtention of {sup 32} P; Diseno y construccion de un prototipo para la obtencion de {sup 32} P  

Energy Technology Data Exchange (ETDEWEB)

In the National Institute of Nuclear Research (ININ) it was designed, built and proved a prototype to obtain {sup 32}P in form of H{sub 3} {sup 32}PO{sub 4}, starting from irradiated S{alpha}. The beginning of the prototype it is based on a distillation system in dry of the S{alpha} in nitrogen atmosphere, and in the formation of the ion {sup 32}PO{sub 4}{sup 3-} in acid solution. Due to the handling of radioactive material during the process, the prototype is inside a hot cell and it has a cylindrical oven that opens up lengthwise to the half, with controller of temperature and with a system of empty air for to transport reagents and products. The air-vacuum system is provided of filters and traps. The tests showed a recovery from 14 to 15% of the activity obtained during the irradiation. (Author)

Alanis M, J. [ININ, Departamento de Materiales Radiactivos, 52045 Ocoyoacac, Estado de Mexico (Mexico)

2003-12-15

306

New method for converting [4-14C]-progesterone into [4-14C]-pregnenolone via the [4-14C]-3?-trimethylsilyloxy-20,20-ethylenedioxy-pregna-3,5-diene  

International Nuclear Information System (INIS)

[4-14C]-Progesterone is converted into [4-14C]-20, 20-ethylene-dioxypregn-4-ene-3-one through the eniminium perchlorate intermediate. Silylation of the ethylenedioxy derivative gives rise to [4-14C]-3?-trimethylsilyloxy-20, 20-ethylenedioxypregna-3,5-diene which is reduced stereospecifically into [4-14C]-3?-hydroxy-20, 20-ethylenedioxy-5-pregnene. Cleavage of the protecting group leads to [4-14C]-pregnenolone with a radiochemical yield of 50% based on [4-14C]-progesterone after purification; specific activity: 59mCi/mM

1975-01-01

307

Metabolismo de fósforo em bovinos: Incorporação de fósforo radioativo (32P) pelos eritrócitos  

Scientific Electronic Library Online (English)

Full Text Available Abstract in portuguese Com o objetivo de avaliar o efeito da suplementação de P sobre a incorporação de 32P pelos eritrócitos de bovinos como método para diagnóstico da deficiência subclínica desse mineral, doze bezerros mestiços pesando em média 191 kg foram separados do rebanho por 12 semanas. Durante as 6 primeiras semanas os animais consumiram apenas a pastagem, sem receber suplementação mineral. Após esse período, os animais foram suplementados com uma mistura mineral comerc (more) ial contendo 6,0% P por mais 6 semanas, permanecendo na mesma pastagem. Amostras de sangue foram coletadas a cada 2 semanas. Quatro ml de sangue total foram incubados a 38°C com 1,85 MBq de 32P por 3 horas. As células foram separadas do plasma por centrifugação e lavadas com solução salina por 3 vezes. A radioatividade nas células foi detectada através do efeito Cerenkov. Os efeitos dos tratamentos foram também investigados sobre os níveis de P e glucose no plasma e P nas fezes. Houve aumento significativo nesses parâmetros à medida em que os animais foram suplementados. As taxas de incorporação de 32P pelos eritrócitos tiveram valores médios de 7,04 ± 1,20 e 5,91 ± 1,24% (P<0,05), para as fases sem e com suplementação de P, respectivamente. O método mostrou ser promissor como um parâmetro adicional na avaliação do estado nutricional de P em ruminantes. Abstract in english To evaluate the effect of phosphorus supplementation on 32P uptake by red blood cells in vitro as diagnosis of subclinical P deficiency, twelve calves averaging 191 kg were separated from the herd for 12 weeks. During the first 6 weeks, the animals were maintained on a pasture without phosphorus supplementation. During the next 6-week period, the animals were supplemented with a commercial mineral salt with 6.0% total P. Four ml of freshly drawn whole blood were obtained (more) at 2-week intervals and incubated with 1.85 MBq 32P. After incubation at 38°C for a 3-hour period, the cells were separated from the plasma and washed three times with an isotonic saline solution and their radioactivity was determined through the Cerenkov effect. Effects of treatment upon the levels of P blood plasma, blood glucose and P excretion were also investigated and these parameters increased significantly with P supplementation. The average rates of 32P uptake by the erythrocytes were 7.04 ± 1.20 and 5.91 ± 1.24% (P<0,05), for treatments without and with P supplementation, respectively. The method showed to be suitable as an additional parameter to estimate the P status in ruminants.

SILVA FILHO, J.C.; VITTI, D.M.S.S.; LOUVANDINI, H.

1997-09-01

308

"In vivo" (35S)methionine interaction with rat liver tRNA.  

UK PubMed Central (United Kingdom)

As part of a study to characterize the methionine role in tumorigenesis, we report that methionine sulfur interacts with rat liver tRNA "in vivo" (35S) radioactivity remained associated to the nucleic acid after a number of treatments, including tRNA deacylation. Similar data were obtained after administration of (methyl-3H) methionine, while no comparable tRNA labelling was detected when the aminoacid labelled in the aliphatic chain was given. Hplc analysis of (35S) tRNA enzymic hydrolysate showed two unidentified UV-absorbing radioactive peaks. NMR spectra of these two peaks did not reveal any thiomethyl group.

Kanduc D; Rossiello MR; Ranieri T; Quagliariello E

1989-08-01

309

Synthetic heparinoids labelled with /sup 125/I and /sup 35/S  

Energy Technology Data Exchange (ETDEWEB)

The labelling of a water-soluble synthetic polyelectrolyte, having anticoagulant activity, has been studied. The polyelectrolyte is derived from cis-1,4-polyisoprene and contains N-sulfate and carboxylate groups. (/sup 125/I)-Iodination of the polyelectrolyte, using the Chloramine-T method and an electrolytic method, resulted in a (/sup 125/I)-labelled polyelectrolyte from which release of the label occurred. Resulfation of a partially desulfated polyelectrolyte with a (/sup 35/S)-sulfur trioxide trimethylamine complex resulted in a (/sup 35/S)-labelled polyelectrolyte which showed no release of the label.

Sederel, L.C.; van der Does, L.; Bantjes, A. (Technische Hogeschool Twente, Enschede (Netherlands)); Kolar, Z. (Interuniversitair Reactor Inst., Delft (Netherlands))

1982-10-01

310

Dating of groundwater with tritium and 14C  

International Nuclear Information System (INIS)

Shallow groundwater can be dated with some accuracy on the basis of its bomb tritium content if the unsaturated soil cover and the aquifer itself is sufficiently homogeneous. A few examples from the Rhine valley are presented. The decrease in tritium level from the water table to a few metres below is nearly two orders of magnitude. Agreement between the measured or estimated variation of bomb tritium in rain during the past decade and the tritium found in shallow groundwater can be obtained if one takes into account that (a) practically no summer rain reaches the water table, and (b) water is mixed by diffusion. Both effects can also be observed in the soil moisture of the unsaturated soil above the water table. Carbon-14 increase in groundwater due to bombs is delayed compared to tritium, the reasons being delay in the biological system and exchange with the carbonate in the soil. Nevertheless lysimeters show a marked increase of 14C, which depends on the plant cover, being high in a plant-covered lysimeter and low in a bare one. A simple model is presented, which allows the evaluation of the influence of exchange on the 14C age obtained. It turns out that the deviation from the true age depends on the ratio of the carbonate content in the aquifer material to the carbonate content of the water, on the specific contact surface or the grain size but not on the groundwater velocity. On the basis of this model the experimental finding that 14C ages are usually in agreement with other age estimates despite the loss by exchange is plausible owing to the fact that only material of sufficiently coarse grain size can make up a reasonable aquifer. Assuming only exchange with a monomolecular surface layer of the carbonate grains one finds that the 14C age is likely to differ by not more than a factor of two in the most unfavourable case. Under natural conditions (steady state of cosmic-ray-produced 14C) the 14C content of shallow groundwater is hardly influenced at all by exchange, even within very finely grained soil. In the case where the 14C level is rising because of bomb testing, a new equilibrium with the monolayer must be established, by which the 14C increase in groundwater is delayed. The extent of carbonate exchange has been studied under laboratory conditions in a column. The delay of the 14C pulse in spiked water under the conditions chosen is such that the spike travels with approximately half the velocity of the water. From this and from the dispersion observed one can conclude that equilibrium with the surface monolayer is reached within a few hours. Further studies at elevated temperatures indicate the extent to which penetration into deep layers must be taken into account. (author)

1967-01-01

311

List of [sup 14]C data 1975 - 1987. Liste der [sup 14]C-Daten 1975 - 1987  

Energy Technology Data Exchange (ETDEWEB)

The results of about 900 [sup 14]C radiocarbon age determinations performed at the Geographic Institute of the Zurich University and the Institute for Medium Energy Physics of the Federal Institute of Technology, Zurich, in the period from 1975 to 1987 are compiled. The samples stemmed mainly from Switzerland but also from abroad. tabs., 128 refs.

Keller, W.A.

1988-01-01

312

Effect of radioactive isotope 32P upon alpha amylase activity and glucose concentration in chickens  

International Nuclear Information System (INIS)

[en] An attempt has been made to investigate whether alpha amylase activity and glucose concentration in blood plasma can serve as the help in establishing on early diagnosis of organic or functional damage caused by ionizing radiation in chickens. Fifty day old hybrid chickens of heavy 'Jata' breeds of both sexes, were treated by 32P administered intramusculary as sodium orthophosphate in a single dose of 333 MBq per kilogram of body weight. Blood samples was taken from the wing vein on day 1, 3, 5, 7 and 10 after administration of 32P. Alpha amylase activity and glucose concentration were determined spectrophotometrically using kits produced by 'Radonja', Sisak. Alpha amylase activity was decreased and glucose concentration was increased during investigated period. Yet, the further investigations are needed to find out whether these two parameters can be used for early diagnosis of injury in chicken organism by ionizing radiation. (author)

1996-01-01

313

Single-well technique using 32P for determining direction and velocity of groundwater flow  

International Nuclear Information System (INIS)

A radiographic method for determining the direction, ?, and the velocity, v, of groundwater flow has been developed. The radioisotope 32P is injected, as a point or a thin-column source, at the centre of the well by means of a simple device. The injection is performed at the desired depth without disturbing the water. The radioisotope is left to follow the horizontal flow of water. Some of the 32P is adsorbed onto the walls of two parallel detecting screens which are separated by a few centimetres and fitted inside the well. Both the inner and outer screens are radiographed; the darkest parts of the emulsions and the north direction marked on another film give the value of ?. The blackening of each film in the screens is measured. Velocities are determined either by the displacement method or from the calibration curve of the velocity versus relative blackening. (author)

1970-01-01

314

Biological fate of 32P malathion in gallus domesticus (Desi poultry birds)  

International Nuclear Information System (INIS)

During this study, a minor surgical technique was developed for the separation of urine and faeces in birds and fate of 32P malathion was studied, following a single oral dose of 394 mg/kg. The birds showed characteristic signs and symptoms of organophosphorus poisoning and the results suggested that the compound is rapidly absorbed from the gastro-intestinal tract, significant quantities being detected in plasma after 0.5 h of ingestion. Highest concentration of 32P in various organs decreased and at 48 h, it was not detected except in liver, kidney, lung and spleen when only traces were observed. The cumulative urinary and faecal excretion study revealed that within 24 h 90% is rapidly excreted mainly via the urine and only small amounts in the faeces. Metabolism studies showed that the compound is quickly metabolised. Because of the rapid turnover of the compound, this study indicated that the accumulation of this compound is unlikely in the body system

1977-01-01

315

Direct determination of the specific activity of RNA uniformly labeled with 32P  

International Nuclear Information System (INIS)

[en] A simple method for the direct determination of the specific activity of RNA uniformly labeled with 32P is described. The procedure is based on the premise that upon disintegration of 32P to 32S, the phosphodiester bond is broken. Analysis of the rate of decay of the full-length molecule by gel electrophoresis and autoradiography can accurately determine the intramolecular specific activity of the RNA. An equation that predicts the relative intensity of the intact RNA molecules remaining as a function of time is presented. These predictions are confirmed using in vitro-synthesized RNA labeled at a known specific activity. This procedure has been used to determine the intramolecular specific activity of RNA labeled in vivo in yeast. It can also be employed to choose the best conditions for experiments utilizing uniformly labeled RNA or single-stranded DNA and requiring the detection of intact molecules

1987-05-01

316

Contribution of Sewage Sludge to Phosphorus Nutrition of Tomato Plants:Using 32P as Tracer  

International Nuclear Information System (INIS)

A pot experiment was conducted to assess the benefits of amending sandy soil with different rates of irradiated and non-irradiated sewage sludge (SS) in terms of its contribution to phosphorus nutrition of tomato plants using 32P as tracer. Soil were amended with four rates of sewage sludge equivalent to 20,40,60 and 80 t/ha. Tomato seedling were transplanted followed by the application of 32P-labelled KH2PO4. Dry matter yield, plant tissue and soil phosphorus concentration and phosphorus uptake were measured after one month of sowing. Dry matter production, plant tissue P concentration and P uptake and soil P have increased significantly as the sludge application rate increased. The increase was higher in irradiated than in non-irradiated sewage sludge, however, the differences aren't rate for both irradiated and non-irradiated sewage sludge.

2001-01-01

317

Preparation of radioactivity labelled DNA. [/sup 32/P-DNA, radiation protection  

Energy Technology Data Exchange (ETDEWEB)

The nick translation reaction, which uses the endonuclease enzyme to incorporate pieces of DNA into the genetic material of other organisms, is being used more and more in Molecular Biology research for the preparation of pure DNA labelled with /sup 32/P. However results are presented which show that high radiation doses are received by the hands of nick translation workers. A Scheme of Work to reduce these doses is described.

Ballance, P.; Morgan, J. (Sussex Univ., Brighton (UK). Safety and Radiation Protection Service); McGregor, G. (Medical Research Council, Brighton (UK). Cell Mutation Unit); Durkacz, B. (Sussex Univ., Brighton (UK). School of Biological Sciences)

1984-03-01

318

Circadian rhythm of pineal uptake of 32P in domestic fowl  

International Nuclear Information System (INIS)

[en] The uptake of radioactive phosphorus by the pineal gland in White Leghorn cockerels (Gallus domesticus) showed a diurnal variation with maxima in the light phase and minima in the dark phase of the light: dark cycle. Constant light caused the rhythm to disappear while constant dark had no effect other than lowering the amplitude of the variations. These data indicate that the rhythm in pineal uptake of 32P is circadian. (author)

1977-01-01

319

Aminotransferase activity in chicken blood plasma after application of a lethal activity of 32P  

Directory of Open Access Journals (Sweden)

Full Text Available An attempt was made to evaluate whether in chicken the activity of plasma aspartate aminotransferase and alanine aminotransferase changes after 32P administration, and whether it helps in the diagnosis of morphological or functional liver damage caused by ionizing radiation before the appearance of clinical simptoms of radiation sickness. Fifty day old hybrid chickens of heavy Jata provenience of both sexes were treated by 32P administred i.m. as disodium hydrogen phosphate in a single dose of 333 MBq per kilogram of body weight. Blood samples were taken from the wing vein on days 1, 3, 5, 7 and 10 after administration of 32P. The activities of aspartate aminotransferase and alanine aminotransferase were determined spectrophotometrically using optimized kits produced by Boehringer Mannheim GmbH. The obtained results have shown that aspartate aminotransferase activity increased on the 3rd and 5th day and it decreased on the 7th and 10th day of the experiment. A statistically significant difference was recorded on the 3rd day of the experiment. Alanine aminotransferase activity increased during the first five days of the experiment, and on the 7th day it decreased. On the 10th day of the experimet the activity of alanine aminotransferase in the blood plasma of 32P treated birds was not detectable; a statistically significant difference was recorded on the 5th day only. The obtained results indicate that the activity of aspartate aminotransferase and alanine aminotransferase may serve as an indicator of functional and/or morphological liver damage in chickens caused by ionizing radiation before the appearance of clinical symptoms of radiation sickness.

Kraljevi? Petar; Šimpraga M.; Vili? M.

2008-01-01

320

Uptake of 32P labelled superphosphate by endomycorrhizal papaya (Carica papaya cv. Coorg honey dew)  

International Nuclear Information System (INIS)

In papaya (Carica papaya cv. Coorg honey dew), there was an increase in 32P uptake and total phosphorus in plants inoculated with mycorrhizal fungi Glomus mossae and G. fasciculatum. Phosphorus derived from fertilizer (Pdff) was lower in mycorrhizal plants while soil derived P, utilisation of P and A values were higher showing thereby that mycorrhizal plants had utilised forms of phosphorus not available to non-mycorrhizal plants. (author). 13 refs., 1 tab

1995-01-01

 
 
 
 
321

Degradation of /sup 14/C-labeled lignins and /sup 14/C-labeled aromatic acids by fusarium solani  

Energy Technology Data Exchange (ETDEWEB)

Abilities of isolate AF-W1 of Fusarium solani to degrade the side chain and the ring structure of synthetic dehydrogenative polymerizates, aromatic acids, or lignin in sound wood were investigated under several conditions of growth substrate or basal medium and pH. Significant transformations of lignins occurred in 50 days in both unextracted and extracted sound wood substrances with 3% malt as the growth substrate and the pH buffered initially at 4.0 with 2,2-dimethylsuccinate. Degradation of lignin in such woods also occurred under unbuffered pH conditions when a basal medium of either 3% malt or powdered cellulose in deionized water was present. Decomposition of the lignin in these woods did not occur in cultures where D-glucose was present as a growth substrate. F. solani significantly transformed, as measured as evolved /sup 14/CO/sub 2/, both synthetic side chain (beta, gamma)-/sup 14/C- and U-ring-/sup 14/C-labeled lignins in 30 days under liquid culture conditions of only distilled deionized water and no pH adjustment. Degradation of dehydrogenative polymerizates by F. solani was reduced drastically when D2 was the liquid medium. AF-W1 also cleaved the alpha-/sup 14/C from p- hydroxybenzoic acid and evolved /sup 14/CO/sub 2/ from the substrace, (3-/sup 14/C) cinnamic acid. Thus, the fungus cleaved side chain carbon from substrate that originally lacked hydroxyl substitution on the aromatic nucleus. Surprisingly, small amounts of /sup 14/C cleaved from aromatic acids by F. solani were incorporated into cell mass. Initial buffering of the culture medium to pH 4.0 or 5.0 with 0.1 M2,2-dimethylsuccinate significantly increased F. solani degradation of all lignins or aromatic acids. Results indicated that AF-W1 used lignin as a sole carbon source.

Norris, D.M.

1980-08-01

322

Radioisotope labelling of several major insect pest. Dipping the pupae in /sup 32/P solution  

Energy Technology Data Exchange (ETDEWEB)

Radioisotope uptake by insects could take place through various parts i.e. mouth, cuticula, intersegmental, secretion and excretion organs. Usually insects are labelled internally by feeding them on an artificial diet containing radioisotope solution. Labelling of several insect pests of cabbage (Crocidolomia binotalis) Zell and Plutella maculipennis Curt and rice (Chilo suppressalis Walker) by dipping of the pupae in /sup 32/P solution showed a promising result. Pupae of Crocidolomia binotalis Zell dipped in 3 ml solution of /sup 32/P with specific activities of 1, 3, 5 and 7 ..mu..Ci/ml had developed labelled adults of sufficiently high radioactivity levels for ecological studies. Similar results were also obtained with Plutella maculipennis Curt and Chilo suppressalis Walker with doses of 1, 3, 5, 7 and 9 ..mu..Ci/ml /sup 32/P solution. The best doses for radioisotope labelling by dipping of the insects Crocidolomia binotalis Zell, Plutella maculipennis Curt, and Chilo suppressalis Walker were 1, 9, and 7 ..mu..Ci/ml respectivelly.

Sutrisno, S. (National Atomic Energy Agency, Jakarta (Indonesia). Pasar Djumat Research Centre)

1981-12-01

323

Novel dose calculation and characterization of {sup 32}P intravascular brachytherapy stent source  

Energy Technology Data Exchange (ETDEWEB)

Derived from AAPM task group No. 60/149 protocol, applicable in treatment planning In this study, the two-dimensional dose distributions in water for a {sup 32}P intravascular brachytherapy stent have been calculated. The pure beta emitter source {sup 32}P which has been coated on Palmaz-Schatz stent is discussed. The dosimetric parameters required by the AAPM TG-60/149 formalism are discussed and calculated. Version 5 of the (MCNP) Monte Carlo radiation transport code was used to calculate the dosimetry parameters around the source. The Monte Carlo calculated dose rate at the reference point is found to be 2.8 Gy/{mu}Ci. Also in this study, the geometry function, G(r,{theta}), radial dose function, g(r), and the anisotropy function, F(r,{theta}), have been calculated at distances from 1.8 to 9 mm. The results of these calculations have been compared with other published calculated and measured values for an actual same source. High dose variants were visible near the {sup 32}P stent surface, but these values decreased with depth in water rapidly. There is an acceptable agreement between the calculated data in this study and other published data for the same source, which validate our simulations method. (orig.)

Sadeghi, Mahdi [Agricultural, Medical and Industrial Research School, Karaj (Iran, Islamic Republic of). Nuclear Science and Technology Research Institute; Kiavar, Omid; Saidi, Pooneh [Islamic Azad Univ., Tehran (Iran, Islamic Republic of). Dept. of Nuclear Engineering; Fatehi, Rozhin [Islamic Azad Univ., Tehran (Iran, Islamic Republic of). Dept. of Chemistry

2011-11-15

324

Effect of 32P treatment for polycythaemia vera on blood lymphocyte subpopulations and their functions  

International Nuclear Information System (INIS)

[en] The influence of 32P treatment on the blood lymphocyte population was examined in 16 patiens with polycythaemia vera who had not previously been treated with cytotoxic drugs or irradiation. Before treatment the lymphocyte counts were within the normal range but the expression of certain membrane structures, as detected by monoclonal antibodies directed against total T cells (CD 3 and 5), helper/inducer (CD 4) and suppressor/cytotoxic T cells (CD 8), were slightly reduced. In addition, mitogenic responses of the lymphocytes to PHA and PWM-induced Ig secretion were severely impaired. Following a single oral dose of 32P (150-305 MBq), which was shown to normalize the production of erythrocytes and/or platelets, the blood lymphocyte counts were reduced by approximately 40% 12 wk after treatment. Subset analysis showed that the proportion of B cells, as identified by monoclonal antibodies (CD 20), was reduced to the highest relative extent. On the other hand, lymphocytes expressing the above T cell markers were somewhat increased. 32P treatment sharply increased PHA reactivity but it further reduced PWM-induced Ig secretion. The latter observation was in line with the finding that serum concentrations of Ig were reduced following treatment. (author)

1987-01-01

325

Preparation of (32)P-end-labeled DNA fragments for performing DNA-binding experiments.  

UK PubMed Central (United Kingdom)

The generation of a uniquely (32)P-end-labeled DNA fragment is essential for DNA-binding experiments such as DNase I footprinting and ethylation interference. We describe here a protocol for end-labeling a restriction fragment. For a plasmid DNA bearing a region containing the binding site of interest, cleaving with a single restriction endonuclease generates a 5' overhang containing a phosphate. This is generally necessary for both common forms of fragment end-labeling: phosphorylation with polynucleotide kinase and "filling in the end" with DNA polymerases (e.g., Klenow fragment). For the phosphorylation reaction, as described here, the phosphate is removed with calf intestinal phosphatase or bacterial alkaline phosphatase, and the resulting free 5'-OH is phosphorylated with polynucleotide kinase and [?-(32)P]ATP. This generates a plasmid labeled at each end with ?-(32)P. The molar amount of plasmid DNA must be below the amount of ATP added to the reaction and the ATP must be of sufficiently high specific activity to generate a fragment labeled to the extent necessary for many DNA-binding experiments. To generate a uniquely end-labeled DNA fragment, the labeled plasmid is heat-treated to inactivate any remaining kinase and recleaved with a second endonuclease, releasing a short DNA fragment and a longer vector fragment. The DNA fragment is purified from the labeled vector on a 5%-8% native polyacrylamide gel. The preparation and labeling of DNA restriction fragments typically takes 1-2 d.

Carey MF; Peterson CL; Smale ST

2013-05-01

326

Preparation of (32)P-end-labeled DNA fragments for performing DNA-binding experiments.  

Science.gov (United States)

The generation of a uniquely (32)P-end-labeled DNA fragment is essential for DNA-binding experiments such as DNase I footprinting and ethylation interference. We describe here a protocol for end-labeling a restriction fragment. For a plasmid DNA bearing a region containing the binding site of interest, cleaving with a single restriction endonuclease generates a 5' overhang containing a phosphate. This is generally necessary for both common forms of fragment end-labeling: phosphorylation with polynucleotide kinase and "filling in the end" with DNA polymerases (e.g., Klenow fragment). For the phosphorylation reaction, as described here, the phosphate is removed with calf intestinal phosphatase or bacterial alkaline phosphatase, and the resulting free 5'-OH is phosphorylated with polynucleotide kinase and [?-(32)P]ATP. This generates a plasmid labeled at each end with ?-(32)P. The molar amount of plasmid DNA must be below the amount of ATP added to the reaction and the ATP must be of sufficiently high specific activity to generate a fragment labeled to the extent necessary for many DNA-binding experiments. To generate a uniquely end-labeled DNA fragment, the labeled plasmid is heat-treated to inactivate any remaining kinase and recleaved with a second endonuclease, releasing a short DNA fragment and a longer vector fragment. The DNA fragment is purified from the labeled vector on a 5%-8% native polyacrylamide gel. The preparation and labeling of DNA restriction fragments typically takes 1-2 d. PMID:23637369

Carey, Michael F; Peterson, Craig L; Smale, Stephen T

2013-05-01

327

Genomic 5-methylcytosine determination by /sup 32/P-postlabeling analysis  

Energy Technology Data Exchange (ETDEWEB)

A simple and sensitive method for the quantitation of 5-methyldeoxycytidine in DNA has been developed by the adaptation of the Randerath /sup 32/P-postlabeling technique. Nucleic acids were digested to 3'-monophosphate nucleotides, which were converted to /sup 32/P-labeled 3',5'-bisphosphate nucleotides, the 3'-phosphate was cleaved by the action of nuclease P1, and the resultant 5'-(/sup 32/P)-monophosphate nucleotides were separated by two-dimensional thin-layer chromatography. Less than 1 microgram of DNA was required for the precise quantitation of 5-methyldeoxycytidine content to a detectable limit of 0.01% of the total cytidine residues methylated. The genomic 5-methyldeoxycytidine content may thus be quantitated in tissue samples, small or selective cell populations, senescing or terminally differentiating cells, or DNA from any source. We report here, for the first time, the genomic 5-methyldeoxycytidine content of normal human bronchial epithelial and normal human pulmonary mesothelial cells. The chromatographic separation of all of the normal and some of the rare monophosphate deoxyribonucleotides and ribonucleotides has been characterized. Thus, 5-bromodeoxyuridine and the RNA contamination of DNA or the DNA contamination of RNA can also be quantitated during the same analysis.

Wilson, V.L.; Smith, R.A.; Autrup, H.; Krokan, H.; Musci, D.E.; Le, N.N.; Longoria, J.; Ziska, D.; Harris, C.C.

1986-02-01

328

Effect of adrenaline on 32 P incorporation into rat fat-cell phospholipids.  

Science.gov (United States)

1. The phospholipid composition of fat-cells prepared from rat epididymal fat-pad was determined. 2. The incorporation of [(32)P]P(i) into the phospholipids of fat-cells incubated in glucose-free medium and the effect of adrenaline and of alpha- and beta-adrenergic blocking agents, were studied. 3. Incorporation of [(32)P]P(i) into fat-cell phospholipid increased with time; incubation with adrenaline resulted in increased incorporation that was related to the concentration of adrenaline. 4. The pattern of incorporation of [(32)P]P(i) into the individual phospholipids of fat-cells after incubation for 1h was determined; adrenaline (5.4mum) resulted in increased incorporation into phosphatidylcholine. 5. Incubation of fat-cells with propranolol (34mum) and adrenaline (5.4mum) resulted in abolition of adrenaline-stimulated lipolysis; there was a decrease in the specific radioactivity of phosphatidylcholine and an increase in the specific radioactivity of phosphatidylethanolamine, phosphatidic acid, phosphatidylinositol and cardiolipin compared with cells incubated with adrenaline alone. 6. Incubation of fat-cells with phenoxybenzamine (0.1mm) and adrenaline (5.4mum) resulted in stimulation of lipolysis, and in diminished specific radioactivities of phosphatidylcholine, phosphatidic acid, phosphatidylinositol, phosphatidylglycerol and choline plasmalogen compared with cells stimulated with adrenaline alone. PMID:4344003

Stein, J M; Hales, C N

1972-07-01

329

Effect of adrenaline on 32 P incorporation into rat fat-cell phospholipids.  

UK PubMed Central (United Kingdom)

1. The phospholipid composition of fat-cells prepared from rat epididymal fat-pad was determined. 2. The incorporation of [(32)P]P(i) into the phospholipids of fat-cells incubated in glucose-free medium and the effect of adrenaline and of alpha- and beta-adrenergic blocking agents, were studied. 3. Incorporation of [(32)P]P(i) into fat-cell phospholipid increased with time; incubation with adrenaline resulted in increased incorporation that was related to the concentration of adrenaline. 4. The pattern of incorporation of [(32)P]P(i) into the individual phospholipids of fat-cells after incubation for 1h was determined; adrenaline (5.4mum) resulted in increased incorporation into phosphatidylcholine. 5. Incubation of fat-cells with propranolol (34mum) and adrenaline (5.4mum) resulted in abolition of adrenaline-stimulated lipolysis; there was a decrease in the specific radioactivity of phosphatidylcholine and an increase in the specific radioactivity of phosphatidylethanolamine, phosphatidic acid, phosphatidylinositol and cardiolipin compared with cells incubated with adrenaline alone. 6. Incubation of fat-cells with phenoxybenzamine (0.1mm) and adrenaline (5.4mum) resulted in stimulation of lipolysis, and in diminished specific radioactivities of phosphatidylcholine, phosphatidic acid, phosphatidylinositol, phosphatidylglycerol and choline plasmalogen compared with cells stimulated with adrenaline alone.

Stein JM; Hales CN

1972-07-01

330

Colloidal chromic phosphate 32P synovectomy in antigen-induced arthritis in the rabbit  

International Nuclear Information System (INIS)

Radioisotopes have been employed in the therapy of chronic arthritis, in particular, rheumatoid arthritis for many years. A variety of isotopes have been popularized, and in the last ten years a colloidal solution of radioactive chromic phosphate 32P has been in use apparently with equivalent efficacy to others such as 169erbium, 90yttrium, and 165dysprosium. No controlled studies on this modality have been reported and few animal studies were found. The efficacy of therapeutic doses of 32P as a medical synovectomy and its effect on rabbit joints with antigen-induced arthritis were observed in 62 arthritic knee joints in 31 adult rabbits treated on one side with 0.1 microCi of 32P, the opposite serving as control. The animals were observed over a period of 11 months and examined by histologic and biochemical means. The synovium showed no evidence of radiation necrosis in treated joints. Cartilage of treated and control joints showed similar changes consistent with chronic arthritis, persistent synovitis, progressive chondrocyte degeneration, and decreased matrix metachromasia. The radiosynovectomy had neither removed synovium nor protected the cartilage. Its efficacy in humans is therefore questionable.

1988-01-01

331

The dosimetry for a coronary artery stent coated with radioactive 188Re and 32P  

International Nuclear Information System (INIS)

Radiation dose distributions have been calculated for 188Re and 32P activity on a coronary artery stent. The doses have been calculated both as a function of position along the stent and of depth into the artery wall. Comparisons of the dose from identical activities of 188Re and 32P on the stent show that the major differences arise from the different half-lives of the two activities. Coating the activity onto three surfaces of the stent rather than just the outside surface is found to reduce the dose by approximately 8 to 9%. Similarly, the effect of ignoring the attenuation in the stainless steel of the stent is to increase doses by 11 to 17%. Consideration is also given to the effect of the prolonged treatment times associated with a radioactive stent compared with the more common treatment over several minutes. It is shown that extended treatment may require between two and eight times the single dose to achieve the same effect depending on factors such as the radionuclide used, the dose required and the assumed cell survival curve. On the assumption that an instantaneous dose of 18 Gy at a depth of 1 mm into the artery would be required for successful prevention of neointimal hyperplasia, activities required for a stent coated with 188Re and 32P are tabulated. (author)

2000-01-01

332

Effects of 35S-dimehypo pesticide on agricultural environment and ecosystem  

International Nuclear Information System (INIS)

Dimehypo is a nereistoxin derivative, which was developed and manufactured in China. In order of appraise its environmeatal safety comprehensively, the radioisotopic tracer technique and other methods were applied to investigate on the effects of 35S-labelled dimehypo (35S-dimehypo) on agricultural environment and ecosystem. The results revealed its low adsorptivity and high mobility, especially water-carrying mobility in soil, fair stability in soil and water, and slowness in degradation. Its main product of degradation, nereistoxin, was of lower mobility than its material compound in soil. 35S-dimehypo could enter the bodies of grass carps Ctenopharyngodon idellus along with bails or via the respiratory tracts, and could be excreted fast after the fish were removed from the contaminated source. Liquid and granulated 35S-dimehypo were fed to quail and fowls respectively, and could also be excreted rapidly in excretion and urine. Its much less distribution coefficient in caprylalcoholwater system suggested no accumulation in the adipose tissue of organism. The release of its effective composition in the granules prepared with porcelain clay or clay soil is prompt and complete

1988-01-01

333

[35S]ATP gamma S binding sites in the purified heart sarcolemma membrane  

International Nuclear Information System (INIS)

Purified heart sarcolemma membranes were found to bind a slowly hydrolyzable analogue of ATP [35S-labeled adenosine 5'-(gamma-thio)triphosphate [(35S]ATP gamma S)] in a specific manner and exhibited two apparent affinity sites. The high-affinity site had a dissociation constant (KD) of 4.7-8.3 nM [maximum binding (Bmax) = 9.5-18.4 pmol/mg protein], whereas the low-affinity site had a KD of 655-1,257 nM (Bmax = 812-2,955 pmol/mg protein). Like ATP, other nucleotides such as GTP, UTP, ITP, and CTP were effective in displacing [35S]ATP gamma S binding. Although crude membrane preparations from different tissues also exhibited both high- and low-affinity sites for [35S]ATP gamma S, KD values for the high affinity sites were severalfold higher than that for the purified heart membranes. It is proposed that the high-affinity binding site for nucleotides may represent the ATP receptor in the heart cell membrane

1990-01-01

334

The uptake of S from four different 35 S labbelled fertilizer by tea plant  

International Nuclear Information System (INIS)

The uptake of S derived from 35-S-labelled ammonium sulphate, potasium sulphate, Kieserit (MgSO4) and Gypsum(CaSO4) of the specific activity of 0.1 mCi/g S by tea clone TRI 2025 planted in andosols was investigated. Randomized block design was used in the experiment. Fertilizers were give once at the rate of 40 kg S/ha. Counting of 35-S samples collected from the youngest and the lower leaves were done every week. Results of the experiment showed that the uptake patterns of S derived from fertilizers were the same for the youngest and the lower leaves. The activity of 35-S was clearly detected in leaves samples after one week of fertilizers application and increased continuously upto eight weeks. The total activity of 35-S in the plucked leaved derived from ammonium sulphate, potasium sulphate and Kieserite were little bit higher than from gypsum. (authors). 10 refs, 6 figs, 2 tabs.

1988-01-01

335

Determination of chemical states of sulphur 35 obtained from the 35Cl (n, p)35S  

International Nuclear Information System (INIS)

The chemical states of sulphur-35 obtained from the 35Cl(n,p)35S reaction by the irradiation of potassium chloride without any previous treatment and with previous heating under vacuum, were determined. The influence of irradiation time and temperature after irradiation was examined. Paper electrophoresis technique was employed for the determination of the chemical states. (Author)

1980-01-01

336

[(35)S]sulfate incorporation into glomerular basement membrane glycosaminoglycans is decreased in experimental diabetes  

International Nuclear Information System (INIS)

[en] Isolated rat renal glomeruli incorporate radioactive sulfate into glycosaminoglycans, which are integral components of the glomerular basement membrane. Cellulose acetate electrophoresis and specific enzymatic sensitivities of glycosaminoglycans prepared after pronase digestion of purified glomerular basement membrane indicate the presence of heparan sulfate. We examined the effect of experimental diabetes on the incorporation of [(35)S]-sulfate into glycosaminoglycans deposited into newly synthesized glomerular basement membrane in vitro. Basement membranes were purified from glomeruli isolated from normal and streptozotocin-diabetic rats after incubation for 2 hr with radiolabeled sulfate and then were subjected to pronase digestion for isolation of the glycosaminoglycans. [(35)S] incorporation into basement membrane glycosaminoglycans was significantly decreased in glomeruli from diabetic animals. The addition of insulin (100 micron U/ml) in vitro did not affect [(35)S] incorporation into glycosaminoglycans of the glomerular basement membranes in normal or diabetic glomeruli. High glucose concentration (5 vs. 20 mM) was without effect in short-term incubations of glomeruli from normal animals. The results indicate that experimental diabetes influences [(35)S] sulfate incorporation into glomerular basement membrane glycosaminoglycans and suggest that decreased heparan sulfate production and/or sulfation may contribute to the increased permeability of the glomerular basement membrane in diabetes

1981-01-01

337

Absolute liquid scintillation counting of 35S and 45Ca using a modified integral counting method  

International Nuclear Information System (INIS)

[en] The disintegration rates of 35S and 45Ca samples were determined by modified integral counting method, which extrapolates the integral count rate to the zero detection threshold of a liquid scintillation spectrometer. The agreement between the extrapolated value and the standardized disintegration rate of the sample is very satisfactory. (author) 6 refs.; 1 fig.; 2 tabs

1994-07-01

338

Proteinases release /sup 35/S-labeled macromolecules from cultured airway epithelial cells  

Energy Technology Data Exchange (ETDEWEB)

To determine whether proteinases release radiolabeled macromolecules from airway cells devoid of secretory granules, they studied canine cultured tracheal epithelial cells grown to confluency. At this time the cells are bound by tight junctions, maintain anion transport, have a well developed glycocalyx, but contain no secretory granules. They labeled the cells with /sup 35/SO/sub 4/ (50..mu..ci/ml/24h) then changed the medium every 20 min and measured nondialyzable /sup 35/S released into the medium. Two h later, the rate of spontaneous release of /sup 35/S-labeled-macromolecules was 5700 +/- 1600 CPM/20 min (mean +/- SD). At this time trypsin, thermolysin, pseudomonas elastase and alkaline proteinase, each released /sup 35/S-labeled-macromolecules, whereas aspergillus acid proteinase did not. In more detailed studies, trypsin released /sup 35/S in a concentration dependent fashion, with a threshold below 10 units/ml and a response to 1000 units/ml of 1092 +/- 173% (mean +/- SD; n=5 cultures) above pre-trypsin baseline. Sepharose CL4B chromatography of the radiolabeled materials released by trypsin showed a void volume fraction (MW greater than or equal to 10/sup 6/), and a second, included fraction (MW 2-3 x 10/sup 5/). These results indicate that cultured airway epithelial cells synthesize macromolecules and release them into the medium, and that proteinases increase the rate of macromolecule release markedly.

Varsano, S.; Borson, D.B.; Gold, M.; Forsberg, S.; Basbaum, C.B.; Nadel, J.A.

1986-03-05

339

[Distribution of 35S labeled sulfated mucopolysaccharides in the recipient eye after corneal allografting. Experimental studies in rabbits  

UK PubMed Central (United Kingdom)

The release of 35S labeled corneal glycoprotein from an allogenic interlamellar corneal graft was studied in randomly selected rabbits. Simultaneously, the uptake of 35S by the recipient animal was investigated. These changes followed for a period of 80 h indicate that there is a constant 35S release fromthe donor cornea and that it is the recipient cornea which takes up the major part of the 35S from the donor. The iris, choroid, and sclera of the recipient also take up a certain percentage of the donor's 35S while the aqueous humor, the optic nerve, and the local lymph nodes take up only minimal amounts.

Stremke G; Meissner J; Böke W

1979-02-01

340

Synthesis of triamcinolone acetonide-4-/sup 14/C  

Energy Technology Data Exchange (ETDEWEB)

The free hydroxyl groups in triamcinolone acetonide (1) were protected as silyl ethers to give the bis-silyloxy derivative 4. Compound 4 was then hydrogenated over 5% palladium on carbon to give the ..delta../sup 4/-3-oxo steroid 5 which was then converted to the enol-lactone 7 under selective reaction conditions. The enol-lactone 7 was subjected to Grignard reaction with /sup 14/C-methylmagnesium iodide to give an adduct 8a which on subsequent treatment with lithium 2,6-di-t-butylphenoxide gave 4-/sup 14/C-..delta../sup 4/-3-oxo derivative 9. Compound 9 was heated with selenium dioxide in t-butanol containing pyridine to give the ..delta..sup(1,4)-3-oxo derivative 10. Removal of the silyl protecting groups was readily accomplished by heating 10 with hydrochloric acid in methanol-tetrahydrofuran solution to give the title compound 11.

Rao, P.N.; Cessac, J.W.; Hill, K.A. (Southwest Foundation for Research and Education, San Antonio, TX (USA))

1982-10-01

 
 
 
 
341

Synthesis of triamcinolone acetonide-4-14C  

International Nuclear Information System (INIS)

[en] The free hydroxyl groups in triamcinolone acetonide (1) were protected as silyl ethers to give the bis-silyloxy derivative 4. Compound 4 was then hydrogenated over 5% palladium on carbon to give the ?4-3-oxo steroid 5 which was then converted to the enol-lactone 7 under selective reaction conditions. The enol-lactone 7 was subjected to Grignard reaction with 14C-methylmagnesium iodide to give an adduct 8a which on subsequent treatment with lithium 2,6-di-t-butylphenoxide gave 4-14C-?4-3-oxo derivative 9. Compound 9 was heated with selenium dioxide in t-butanol containing pyridine to give the ?sup(1,4)-3-oxo derivative 10. Removal of the silyl protecting groups was readily accomplished by heating 10 with hydrochloric acid in methanol-tetrahydrofuran solution to give the title compound 11. (author)

1982-01-01

342

4He decay of excited states in 14C  

CERN Multimedia

A study of the 7Li(9Be,4He 10Be)2H reaction at E{beam}=70 MeV has been performed using resonant particle spectroscopy techniques and provides the first measurements of alpha-decaying states in 14C. Excited states are observed at 14.7, 15.5, 16.4, 18.5, 19.8, 20.6, 21.4, 22.4 and 24.0 MeV. The experimental technique was able to resolve decays to the various particle bound states in 10Be, and provides evidence for the preferential decay of the high energy excited states into states in 10Be at ~6 MeV. The decay processes are used to indicate the possible cluster structure of the 14C excited states.

Soic, N; Donadille, L; Clarke, N M; Leask, P J; Catford, W N; Jones, K L; Mahboub, D; Fulton, B R; Greenhalgh, B J; Watson, D L; Weisser, D C

2003-01-01

343

4He decay of excited states in 14C  

International Nuclear Information System (INIS)

A study of the 7Li(9Be,4He10Be)2H reaction at Ebeam=70 MeV has been performed using resonant particle spectroscopy techniques and provides the first measurements of ?-decaying states in 14C. Excited states are observed at 14.7, 15.5, 16.4, 18.5, 19.8, 20.6, 21.4, 22.4, and 24.0 MeV. The experimental technique was able to resolve decays to the various particle bound states in 10Be and provides evidence for the preferential decay of the high energy excited states into states in 10Be at ?6 MeV. The decay processes are used to indicate the possible cluster structure of the 14C excited states

2003-01-01

344

Fate of 14C-monocrotophos in lactating goats  

International Nuclear Information System (INIS)

14C-monocrotophos was fed daily to two groups of lactating goats for five days at 5 and 10 mg per goat per day. The radioactivity in urine and faeces accounted for 50% and 7%, respectively, 24 hours after the first dose. About 25% of the radiocarbon was found in various organs. About 1-2% of the administered radiocarbon was secreted in milk. It was concluded that monocrotophos was rapidly eliminated through urine and faeces of teddy goats. 14C-residues in milk and urine were tentatively identified as monocrotophos, dimethylphosphate and N-desmethyl monocrotophos. The erythrocyte cholinesterase activity was reduced to 50% 24 hours after treatment, but no toxic symptoms were observed. (author)

1987-01-01

345

Residues of {sup 14}C-paclobutrazol in mangoes  

Energy Technology Data Exchange (ETDEWEB)

Paclobutrazol (PBZ) is a growth regulator used in agricultural systems whose purpose is the control of vegetative growth, stimulating the reproductive capacity of plants. This growth regulator remains active in soil for a long time and its half-life varies with the type of soil and climatic conditions, can severely affect the development of crops. This work aimed to study the residues / metabolites of {sup 14}C-PBZ in mango pulp Tommy Atkins. The tests were performed with mangoes grown in pots stainless steel and application of {sup 14}C-PBZ was performed by the soil projection of the crown, and the mangoes tested in two periods, one year and two years after application. To evaluate the levels of residues of {sup 14}C-PBZ was realize the burning of 200 mg of pulp on biological oxidized and detached {sup 14}CO{sub 2} was detected by liquid scintillation spectrophotometer. The results were 1.65 % of residue of PBZ on fruit collected after two years of application and 4.30 % of residue of PBZ collected on fruit after a year of application and also can see that the product remained in the soil for more than one year, is translocated to the plant and reach the edible part, the pulp fruit. The identification of residual {sup 14}C- PBZ/metabolites by thin-layer chromatography did not reveal any pattern of PBZ / metabolites due to the low activity detected in the samples. Therefore, another procedure was performed for extraction and then analyzed by high performance liquid chromatography (HPLC) for detection of metabolites in the PBZ of mango pulp. (author)

Costa, Maria A.; Tornisielo, Valdemar L.; Castanho, Giuliane M., E-mail: macosta@cena.usp.b [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil). Lab. de Ecotoxicologia

2009-07-01

346

Residues of 14C-paclobutrazol in mangoes  

International Nuclear Information System (INIS)

Paclobutrazol (PBZ) is a growth regulator used in agricultural systems whose purpose is the control of vegetative growth, stimulating the reproductive capacity of plants. This growth regulator remains active in soil for a long time and its half-life varies with the type of soil and climatic conditions, can severely affect the development of crops. This work aimed to study the residues / metabolites of 14C-PBZ in mango pulp Tommy Atkins. The tests were performed with mangoes grown in pots stainless steel and application of 14C-PBZ was performed by the soil projection of the crown, and the mangoes tested in two periods, one year and two years after application. To evaluate the levels of residues of 14C-PBZ was realize the burning of 200 mg of pulp on biological oxidized and detached 14CO2 was detected by liquid scintillation spectrophotometer. The results were 1.65 % of residue of PBZ on fruit collected after two years of application and 4.30 % of residue of PBZ collected on fruit after a year of application and also can see that the product remained in the soil for more than one year, is translocated to the plant and reach the edible part, the pulp fruit. The identification of residual 14C- PBZ/metabolites by thin-layer chromatography did not reveal any pattern of PBZ / metabolites due to the low activity detected in the samples. Therefore, another procedure was performed for extraction and then analyzed by high performance liquid chromatography (HPLC) for detection of metabolites in the PBZ of mango pulp. (author)

2009-10-02

347

Synthesis of 14C-labelled fluphenazine esters  

International Nuclear Information System (INIS)

[en] Fluphenazine labelled with 14C at the 3-propyl position was prepared. Starting from K14CN, 3-chloropropyl p-toluenesulfonate (7) was synthesized via 3-chloropropionic acid (4) intermediate and coupled with 2-trifluoromethyl phenothiazine. The phenothiazine derivative (8) obtained was converted into la, from which various long acting esters (1b, 1c, 1d) of labelled fluphenazine were prepared for comparative pharmacokinetic studies. (author)

1982-01-01

348

Synthesis of /sup 14/C-labelled fluphenazine esters  

Energy Technology Data Exchange (ETDEWEB)

Fluphenazine labelled with /sup 14/C at the 3-propyl position was prepared. Starting from K/sup 14/CN, 3-chloropropyl p-toluenesulfonate (7) was synthesized via 3-chloropropionic acid (4) intermediate and coupled with 2-trifluoromethyl phenothiazine. The phenothiazine derivative (8) obtained was converted into la, from which various long acting esters (1b, 1c, 1d) of labelled fluphenazine were prepared for comparative pharmacokinetic studies.

Zolyomi, G.; Toth, I.; Toldy, L. (Institute for Drug Research, Budapest (Hungary))

1982-06-01

349

Synthesis of [16-14C]trichodiene the precursor to trichothecenes  

International Nuclear Information System (INIS)

The synthesis of radiolabelled trichodiene with 14C at the olefinic methyl (C-16) is described. The isotope was introduced using a 14C-Wittig reagent prepared in situ from triphenyl phosphine and 14C-methyliodide. (author).

1992-01-01

350

Radioactive skin bandages incorporating 90Y, 188Re and 32P for treatment of superficial tumors  

International Nuclear Information System (INIS)

[en] Full text: Radioactive bandages incorporating b-emitters 90Y, 188Re and 32P were prepared with an aim to have a radiation source that will deliver a uniform radiation dose to the site of tumor treatment. There have been reports of specially designed b- emitting paper and skin patches incorporating high-energy beta emitters such as 188Re and 166Ho. 90Y-ferric hydroxide macroaggregates, 188Re-Tin particles and 32P-Chromic phosphate particles were prepared and separately filtered through Millipore filters. Filters incorporating 90Y, 188Re and 32P activity were immobilized between nitrocellulose membranes and placed on adhesive bandage. In-vitro stability of bandages was tested in saline and no leakage of activity was observed from the bandages. 90Y bandages were tested on fibrosarcoma bearing Swiss mice and 188Re bandages and 32P bandages were tested in melanoma bearing C57BL/6 mice. Radioactive bandages were applied on tumors and parameters such as dose, fractionation of dose and time of contact were varied and effect was studied by following the tumor size. At ?18 MBq, there was no significant effect on tumor growth even on placing the patch for 24h. But, animals treated with >37MBq for 3h, showed delay in rate of growth compared to the controls. The best effect was observed on treatment with a second dose of 37-74 MBq after a week, when the tumors regressed fully. In those animals, skin surface appeared hard and necrotic but reversed to normal in few days. Histology studies were done to observe the effect on skin epithelium. Tumor histology slides from treated animals showed regression of tumor. Loss of hair follicles and sebaceous glands was observed in the treated area. Autoradiography and gamma camera imaging of radioactive bandage showed uniformity of dose distribution. Electron Gamma Shower Code (EGS4) system was used to calculate the dose rate distribution due to radioactive bandages at various depths from skin surface. Dose rate calculations revealed that reasonably high dose of ? 2.2 Gy/h/MBq is imparted by radioactive bandages to the tumor site. Both fibrosarcoma and melanoma responded well to treatment with radioactive bandages. Tumor growth was delayed at doses >37MBq. Fractionated doses were effective in complete regression of the tumor. Radioactive bandages incorporating beta emitters such as 90Y, 188Re and 32P could be a promising modality for treatment of superficial tumors. (author)

2004-01-01

351

Behaviour of 14C-DDT in Sudan Gezira soil  

International Nuclear Information System (INIS)

Field cylinders as described in the model experiment for determination of 14C-DDT in Sudan Gezira soil were prepared and samples were collected for determination of 14C-activity at various intervals. About 83-87% of the applied radioactivity was recovered in the extracts from the top 10 cm layer of soil. The loss rate of the chemical from soil increased with time apparently by volatilization and thermal degradation due to high soil temperature, intensive solar radiation and low soil organic matter. This resulted in a half-life of approx. 5 weeks. The major part of the chemical and possible metabolites were detected in the top 10 cm layer. Approximately 8-10% of the applied 14C was detected in samples collected after 16-20 weeks. TLC and autoradiography indicated the presence of p,p'-DDT and p,p'-DDE as the major metabolite. Also traces of TDE were detected in some samples. These results showed that under Sudanese tropical conditions, DDT dissipates very rapidly in soils compared to dissipation in temperate regions. (author). 6 refs, 2 tabs

1988-01-01

352

Biodegradation of 14 C-atrazine under outdoor conditions  

International Nuclear Information System (INIS)

Uniformly 14 C-ring labeled atrazine (5 L/ha) was applied to a Typical Hapludox Brazilian Soil sample which was incubated under outdoor conditions. Sample of 200 g (dry weight base) of fresh soil were distributed in Erlenmeyer flasks and the moisture was adjusted for 2/3 rds of the soil field capacity. The flasks were then buried in the Lysimeter Station when they were incubated. The experiment started jointly with a corn planting. The 14 C O2 was analyzed every 15 day, during a period of 150 days. The desorbed, extracted and bound residues were analyzed. The extracted soil was fractionated and the residues in the humin, fulvic and humic acids were determined. At the end of the incubation period (150 days), the 14 CO2 evolved reached up to 36% of the total applied activity, the bound residues were detected in about the same (34%) during the inoculation period, and were distributed in the fractions of fulvic acids (29,91%), humic acids (6,83%) and humin (63,26%). The metabolites formed in the desorbed residues and in the extracted residues were determined using thin layer chromatography with 14 C-detector. After 150 days incubation, desorbed soil residues were identified as atrazine (52,72%), hydroxiatrazine (44%) and desisopropilatrazine (3,28%). The extractable residues contained atrazine (79,29%), hydroxiatrazine (16,22%), desisopropilatrazine (2,25%) and desetylatrazine (2,24%). (author)

1997-01-01

353

Design and construction of a prototype for the obtention of 32P  

International Nuclear Information System (INIS)

[en] The 32 P are a pure emitting radioisotope of maximum energy of 1.71 MeV with half life of 14.28 days that he has applications in the industry, in agriculture, in medicine, in biology and in ecology (6,11,12,13,17,21,22,23,24,25,26,27,28,29,30,31,32 33).The 32 P can be used in the industry like radiotracer in the investigation of some operations and processes and as element of measurement of some industrial meters. In agriculture is used as radiotracer (29) in the investigation of diverse biological processes that have to do with the production of diverse nutritious products. In medicine has uses but very therapeutic mainly in the treatment of some become cancerous (28, 31, 25, 27) in the diagnosis of blood disorders (24) and like part of materials of production of aortic prosthesis (6). The 32 P are also used in the molecular investigation in biology and in genetics (33), and in studies of ecosystems (32) and of DNA (22). One can obtain the 32 P by means of diverse nuclear reactions depending on the material used as matter it prevails, since it doesn't exist in the nature. But anyone in the ways of obtaining it it should imply a process of radiochemical separation that involves so many steps like they are required, depending on the material used as matter prevails, of the purity with which he wants himself to obtain and of the resources that it has available. The objective of this work was design, to build and to prove a prototype to obtain the 32 P to leave of the irradiation of S ? with fast neutrons at experimental level, which implies also to design a process that contemplates diverse stages and procedures for each one of them. The process was outlined in five stages: matter purification prevails, preparation of irradiation capsules, irradiation of irradiation capsules, transport and opening capsules of irradiation and radiochemical separation. In the last stage it was where uses the prototype of radiochemical separation in basis to the outlined process and considering aspects of environmental and personal radiological safety, in such a way that the radiochemical separation carried out in two stages in the same prototype and with the less manipulation of the radioactive material.To know the functionality of the process and of the prototype, they were carried out three tests of these, in each one of those which the stages of the process were evaluated by means of mensuration of physicochemical parameters and by means of characterizations of the materials involved in these stages with diverse technical analytic instrumental: X-ray diffraction, X-ray fluorescence, scanning electron microscopy, high-purity Ge semiconductor detector proportional detector proportional of low bottom gas and liquid scintillation detector. The results of the process for stages showed that with the outlined method of purification of the matter prevails six of nine constituent elements they were removed of chemical sludges of the raw materials of the process, S ?, and that with the process and the prototype of radiochemical separation it was reached to separate 32 P of the S ?. In general, it was reached to obtain 32 P from S ? irradiated with relatively small quantities of radiochemical impurities. (Author)

2003-01-01

354

Biotechnological production of high specific activity L-35S-cysteine and L-35S-methionine by using a diploid yeast Saccharomyces cerevisiae  

International Nuclear Information System (INIS)

High specific activity L-3 5 S-cysteine and L-35 S-methionine were synthesised by using a wild type diploid strain of baker's yeast-Saccharomyces cerevisiae. Yeast cells were grown in a sulphur depleted synthetic medium in which Na2 3 5 SO4 (50 mCi/ml) was supplemented as the sole sulphur source. The level of incorporation was 60% on an average. The protein hydrolysate of the cultured cells was subjected to paper and column chromatographic separations to get the individual L-3 5 S-aminoacids. The radiochemical yields of cysteine and methionine were 6-7% and 18-20% respectively. The radiochemical purity of the products was >95%. The highest specific activity for the products obtained by employing this method was 1100 Ci/mmole from the starting material, Na2 35 SO4, with a specific activity of 1350 Ci/mmole. (Author).

1994-01-01

355

Migration of 14C in the paddy soil-to-rice plant system after 14C-acetic acid breakdown by microorganisms below the plow layer  

International Nuclear Information System (INIS)

Migration of 14C derived from 14C-acetic acid was examined by using soils sampled from paddies in four administrative areas in Japan (Aomori, Yamanashi, Ehime and Okinawa) and rice plant in a tracer experiment to understand the fate of 14C in the paddy soil-to-rice plant system. The loss of 14C radioactivity levels derived from 14C-acetic acid was caused by soil microorganism breakdown. A part of the 14C fixation to soil was caused by microbial assimilation into the fatty acid fraction. 14C moved upward via two different types of 14C dynamics in soil: quick movement upward; and constant but slow movement upward. 14C was highly assimilated into the plant panicle and that was caused by the root-uptake and the transfer of 14C. Migration of 14C derived from 14C-acetic acid relied heavily upon changes of chemical forms and characteristics of 14C-compound as caused by microorganisms in soil.

2010-01-01

356

Persistence of the herbicides [14C]chlorsulfuron and [14C]metsulfuron methyl in prairie soils under laboratory conditions  

International Nuclear Information System (INIS)

[en] Metsulfuron methyl, whose structure is closely related to that of chlorsulfuron, is currently being evaluated on the Canadian prairies as a postemergence treatment for the control of broadleaf weeds in cereal crops, in non-crop land and for brush control. Although applied postemergence, some of the herbicide will come into contact with the soil making it necessary to determine the fate of metsulfuron methyl in the soil. These studies were undertaken to investigate the rate of breakdown and the fate of [14C]metsulfuron methyl in three soils under laboratory conditions where no leaching could occur. The rate of breakdown of [14C]chlorsulfuron was also investigated in one of the soils

1986-01-01

357

Amount and identity of [14C] residues in bluegills (Lepomis macrochirus) exposed to [14C]triclopyr  

International Nuclear Information System (INIS)

[en] The level and identity of [14C] residues in bluegills (Lepomis macrochirus) exposed to 2.5 mg/L [14C] triclopyr (3,5,6-trichloro-2-pyridinyloxyacetic acid) have been determined. The highest level of radioactivity observed in the flesh of a fish at any time point (0.13 mg/kg, calculated as equivalent mg triclopyr/kg fish) was less than 5% of the fish exposure level of 2.5 mg/L, while the maximum level in the remainder (head, skin, and viscera) was about 95% (2.33 mg/kg) of the fish exposure level, indicating no concentrating effect. The principal components observed in the fish tissues were triclopyr, 3,5,6-trichloro-2-pyridinol, 2-methoxy-3,5,6-trichloropyridine and a conjugate. These components accounted for greater than 75% of all the residues observed

1987-01-01

358

Uptake of (2-/sup 14/C)abscisic acid and distribution of /sup 14/C in apple embryos  

Energy Technology Data Exchange (ETDEWEB)

Pyrus malus L. var. Golden delicious embryos were incubated with (+-)-(2-/sup 14/C) abscisic acid (ABA). After incubations of various durations, the radioactivity was measured in whole embryos, cotyledons, and embryonic axes. With either 48-h or 16-d incubation periods, the uptake of (/sup 14/C) ABA depended upon the mode of culture used. The lowest values corresponded to the absorption by the embryonic axis, the highest to the absorption by the distal parts of the two cotyledons. The cotyledons accumulated the main part of the radioactivity under all conditions. Dormant and almost completely after-ripened embryos cultivated for 4 d showed no significant differences in the radioactivity uptake for identical modes of culture. There was a linear relationship between exogenous ABA concentrations (0.5 to 3.10/sup -5/ M) and ABA uptake for embryos cultivated for 4 d with the distal parts of the cotyledons immersed in the medium.

Barthe, P.; Bulard, C.

1981-01-01

359

Uptake of [2-14C]abscisic acid and distribution of 14C in apple embryos  

International Nuclear Information System (INIS)

Pyrus malus L. var. Golden delicious embryos were incubated with (+-)-[2-14C] abscisic acid (ABA). After incubations of various durations, the radioactivity was measured in whole embryos, cotyledons, and embryonic axes. With either 48-h or 16-d incubation periods, the uptake of [14C] ABA depended upon the mode of culture used. The lowest values corresponded to the absorption by the embryonic axis, the highest to the absorption by the distal parts of the two cotyledons. The cotyledons accumulated the main part of the radioactivity under all conditions. Dormant and almost completely after-ripened embryos cultivated for 4 d showed no significant differences in the radioactivity uptake for identical modes of culture. There was a linear relationship between exogenous ABA concentrations (0.5 to 3.10-5 M) and ABA uptake for embryos cultivated for 4 d with the distal parts of the cotyledons immersed in the medium. (orig.).

1981-01-01

360

Phosphorus absorption (/sup 32/P) by apple trees under drip irrigation as influenced by the physical properties of the soil  

Energy Technology Data Exchange (ETDEWEB)

P absorption by apple tree roots (Golden delicious/M2) is studied using carrier-free /sup 32/P. A qualitative model of the influence of some physical properties of the soil is proposed combining individual tree responses to /sup 32/P injection.

Habib, R. (Institut National de la Recherche Agronomique (INRA), 84 - Montfavet (France). Station d' Agronomie)

1983-01-01

 
 
 
 
361

Phosphorus absorption (32P) by apple trees under drip irrigation as influenced by the physical properties of the soil  

International Nuclear Information System (INIS)

P absorption by apple tree roots (Golden delicious/M2) is studied using carrier-free 32P. A qualitative model of the influence of some physical properties of the soil is proposed combining individual tree responses to 32P injection. (orig.).

1983-01-01

362

Characterization of excited states of 15N through 14C(p,p) 14C using polarized proton beam  

Scientific Electronic Library Online (English)

Full Text Available Abstract in spanish Se midió la distribución angular de la sección eficaz diferencial y el poder analizador para la dispersión elástica de un haz de protones polarizados de entre 3.7 y 11 MeV de energía sobre 14C con pasos muy finos. El análisis de los datos en términos de ondas parciales y corrimientos de fase de la matriz "S" nos permite deducir los números cuánticos y parámetros de diez estados del 15N entre 13.7 y 21.0 MeV de energía de excitación. Las asignaciones previamente publicadas para estos niveles son discutidas. Abstract in english Angular distributions of cross sections and analyzing powers were measured for 14C(p, p)14C for proton energies between 3.7 and 11.0 MeV in very small energy steps. A number of strong resonances are seen. Phase-shift analysis of the elastic scattering data yielded level parameters of eleven states in 15N in the excitation energy region 13.7-21.0 MeV. Previous assignments of spin, parity and energy of levels are discussed.

Murillo, G.; Fernández, M.; Ramírez, J.; Mejia-Gil, M.G.; Policroniades, R.; Varela, A.; Darden, S.E.; Sen, S.; Prior, R.M.; Chávez, E.

2011-02-01

363

Influence of hydroxypropyl-?-cyclodextrin on the biodegradation of 14C-phenanthrene and 14C-hexadecane in soil  

International Nuclear Information System (INIS)

Soil was spiked with [9-14C]phenanthrene and [1-14C]hexadecane at 50 mg kg-1 and aged for 1, 25, 50, 100 and 250 d. At each time point, the microcosms were amended with aqueous solutions of cyclodextrin (HP-?-CD) at a range of concentrations (0-40 mM). Mineralisation assays and aqueous HP-?-CD extractions were performed to assess the effect of the amendments on microbial degradation. The results showed that amendments had no significant impact on the microbial degradation of either of the 14C-contaminants. Further, HP-?-CD extractions were correlated with the mineralisation of the target chemicals in each of the soil conditions. It was found that the HP-?-CD extraction was able to predict mineralisation in soils which had not been amended with cyclodextrin; however, in the soils containing the HP-?-CD, there was no predictive relationship. Under the conditions of this study, the introduction of HP-?-CD into soils did not enhance the biodegradation of the organic contaminants. - The amendment of HP-?-CD to phenanthrene and hexadecane amended soils does not enhance microbial biodegradation.

2009-01-01

364

Practical synthesis of [sup 14]C S-ribosyl-L-homocysteine uniformly labelled on the sugar moiety. An enzymatic route from (U-[sup 14]C) adenosine  

Energy Technology Data Exchange (ETDEWEB)

[(U-[sup 14]C) S-Ribosyl]-L-homocysteine has been prepared enzymatically from (U-[sup 14]C) adenosine in two steps using S-adenosyl homocysteine hydrolase and bacterial S-adenosyl homocysteine nucleosidase as catalysts. (Author).

Guillerm, G.; Allart, B. (U.F.R. Sciences de Reims (France). Lab. de Chimie Bioorganique)

1992-09-01

365

Practical synthesis of 14C S-ribosyl-L-homocysteine uniformly labelled on the sugar moiety. An enzymatic route from (U-14C) adenosine  

International Nuclear Information System (INIS)

[(U-14C) S-Ribosyl]-L-homocysteine has been prepared enzymatically from (U-14C) adenosine in two steps using S-adenosyl homocysteine hydrolase and bacterial S-adenosyl homocysteine nucleosidase as catalysts. (Author).

1992-01-01

366

The influence of 14C-urea breath test on environment  

International Nuclear Information System (INIS)

14C-urea breath test (14C-UBT) is introduced briefly, which shows that 14C-UBT is a excellent noninvasive method for detecting helicobacter pylori (Hp) with safety, simplicity, and speed. The amount of 14C released to environment by practising 14C-UBT is much less than that of 14C in environment from both natural and artificial sources. The 14C released by 14C-UBT with 27.75 kBq each time and 107 times per year is only 0.028% of 14C generated from natural sources per year, if this quantity of 14C-UBT is used per year for ever, it brings an individual person to bear an extra effective radiation dosage of 3.33 x 10-3 ?Sv. So the influence of 14C-UBT on environment could be negligible

2004-01-01

367

Radioactive sputter cathodes for 32P plasma-based ion implantation  

International Nuclear Information System (INIS)

The development of clinical treatments involving the use of beta-emitting millimetric and sub-millimetric devices has been a continuing trend in nuclear medicine. Implanted a few nanometers below the surface of endovascular implants, seeds or beads, beta-emitting radioisotopes can be used in a variety of biomedical applications. Recently, new technologies have emerged to enable the rapid and efficient activation of such devices. A pulsed, coaxial electron cyclotron resonance plasma reactor was designed and tested to demonstrate the feasibility of plasma-based radioactive ion implantation (PBRII). It has been shown that such plasma reactors allow for the implantation of radioisotopes (32P) into biomedical devices with higher efficiencies than those obtained with conventional ion beams. Fragments containing radioactive atoms are produced in the implanter by means of a negatively biased solid sputter cathode that is inserted into an argon plasma. Dilute orthophosphoric acid solutions (H3 32PO4) are used for the fabrication of flat sputter targets, since they offer a high radioisotope content. However, the aggregation of the radioactive solute into highly hygroscopic ring-like deposits rather than flat, thin radioactive films is observed on certain substrates. This article describes the effect of this nonuniform distribution of the radioisotopes on the efficiency of PBRII, and presents a technique which enables a better distribution of 32P by coating the substrates with iron. The iron coating is shown to enable optimal radioisotope sputtering rates, which are essential in 32P-PBRII for the efficient activation of millimetric biomedical devices such as stents or coils.

2006-01-01

368

Radial 32P ion implantation using a coaxial plasma reactor: Activity imaging and numerical integration  

International Nuclear Information System (INIS)

Beta-emitting biomedical implants are currently employed in angioplasty, in the treatment of certain types of cancers, and in the embolization of aneurysms with platinum coils. Radioisotopes such as 32P can be implanted using plasma-based ion implantation (PBII). In this article, we describe a reactor that was developed to implant radioisotopes into cylindrical metallic objects. The plasma first ionizes radioisotopes sputtered from a target, and then acts as the source of particles to be implanted into the biased biomedical device. The plasma therefore plays a major role in the ionization/implantation process. Following a sequence of implantation tests, the liners protecting the interior walls of the reactor were changed and the radioactivity on them measured. This study demonstrates that the radioactive deposits on these protective liners, adequately imaged by radiography, can indicate the distribution of the radioisotopes that are not implanted. The resulting maps give unique information about the activity distribution, which is influenced by the sputtering of the 32P-containing fragments, their ionization in the plasma, and also by the subsequent ion transport mechanisms. Such information can be interpreted and used to significantly improve the efficiency of the implantation procedure. Using a surface barrier detector, a comparative study established a relationship between the gray scale of radiographs of the liners, and activity measurements. An integration process allows the quantification of the activities on the walls and components of the reactor. Finally, the resulting integral of the 32P activity is correlated to the sum of the radioactivity amounts that were sputtered from radioactive targets inside the implanter before the dismantling procedure. This balance addresses the issue of security regarding PBII technology and confirms the confinement of the radioactivity inside the chamber.

2004-01-01

369

Preparation of ( sup 35 S)sulfobromophthalein of high specific activity  

Energy Technology Data Exchange (ETDEWEB)

Study of the hepatocyte transport mechanism of organic anions such as bilirubin and sulfobromophthalein has been limited by the relatively low specific activities of these ligands. ({sup 3}H)Bilirubin and ({sup 35}S)sulfobromophthalein have been available with specific activities of only approximately 100 mCi/mmol. We now report a relatively simple procedure to prepare ({sup 35}S)sulfobromophthalein at a specific activity of approximately 3000 mCi/mmol. This compound is radiochemically pure and serves as a tracer for authentic sulfobromophthalein as judged by chromatography, hepatocyte uptake, metabolism, and biliary excretion. Use of this material as a photoaffinity probe and as a transported ligand may permit dissection and understanding of its transport mechanism.

Kurisu, H.; Nilprabhassorn, P.; Wolkoff, A.W. (Albert Einstein College of Medicine, Bronx, NY (USA))

1989-05-15

370

Uptake of /sup 35/S sulphate by Xenopus cartilage. The influence of growth hormone and prolactin  

Energy Technology Data Exchange (ETDEWEB)

Hypohysectomized juvenile Xenopus were injected with growth hormone (GH) or prolactin (PRL) of either ovine or bullfrog origin. The growth promoting activity of these hormones was measured by monitoring the uptake of /sup 35/S sulphate by the xiphisternal cartilage in vitro. Analysis of the labelled cartilage revealed that the acid mucopolysaccharide fraction contained about 60-80 % of the label most of which was incorporated into chondroitin sulphates. All of the hormones tested enhaced the /sup 35/S sulphate uptake dose-dependently. Among them bullfrog GH was most effective, then followed ovine GH and ovine PRL. Bullfrog PRL was far less effective than other three. The sensitive assay for frog GH developed in the present experiment may be applicable to the assay for somatomedin-like activity and contribute to the analysis of the mode of action of GH in amphibians.

Ishii, Takehisa; Kikuyama, Sakae (Waseda Univ., Tokyo (Japan))

1984-08-01

371

Root activity studies of guava plants (Psidium Quajava, L) using 32P radioisotope  

International Nuclear Information System (INIS)

The study was conducted on two year old guava plants (Psidium guajava L.) grown on Bungor series soil (Typic paleudult) at the UTN Agricultural Experimental Plot, Bangi, Selangor. The active roots of these guava plants were found to be present in abundance at about 100 cm away from the base of the tree at a depth of 10 cm. Fertilizers could be applied in that area. Sampling of every fourth leaf (for young trees) for counting, to represent the 32P counts in the leaves can be used in future studies. The leaves and branches (green parts) of guava also have a more consistent P contents, as compared to other parts. (author)

1988-01-01

372

Braquiterapia del Espacio Subaracnoideo y Ventrículos Cerebrales con el Radiocoloide Fosfato Crómic 32P (CROP).  

Directory of Open Access Journals (Sweden)

Full Text Available Se desarrolló un método de Braquiterapia del espacio subaracnoideo mediante la introducción de un coloide de FostatoCrómico 32P (CROP) en el LCR. Se usaron 90 conejos y los coloides fueron inyectados por vía cisternal. Se comprobó la distribución uniforme y permanencia del CROP en la pia-aracnoides de todo el espacio subaracnoideo y superficie cortical y en el epéndimo ventricular mediante autorradiografías y cortes histológicos con técnica de stripping film. La distribución del 32P en hígado (10%), bazo (7%) y médula ósea (2%) y otros órganos no provoca efectos significativos. Se comprueba la tolerancia neurológica perfecta para los coloides de partículas finas de menos de 100 nm (CROP), y la neurotoxicidad de los coloides de partículas grandes mayores a 100 nm.. Con altas dosis los estudios histológicos demostraron la ausencia de edema o de lesiones en células y tejido nerviosos. Hasta 6 meses de observación ninguno de los animales mostró trastornos neurológicos atribuíbles al CROP, ni con la repetición de sus aplicaciones.A method of brachitherapy of the CNS with Chromic Phosfate (32P) in colloidal form (CROP) introduced in the subarachnoid space was developed. 90 rabbits were used and colloids were injected through a cysternal tap The permanence and uniform distribution of the colloid in the pio-arachnoid membrane along the whole subarachnoidal space and ventricular ependyma was asserted with autoradiographs and histological sections with stripping film techniques. The distribution of 32P in liver (10%), spleen (7%) and bone marrow (2%) and other organs has no significant effects. The perfect tolerance to the injection of colloids of fine particles with less than 100 nm, (CROP) was proved and no severe neurotoxic effets where observed. When colloids with particles larger than 100 nm were used this undesirable effects were seen. With very high doses, no edema nor lesions could be demonstrated in histological studies of nervous cells or nervous tissue. Up to 6 months the animals did not present neurological symptoms attributable to CROP in that period, even with repeated applications.

Girado, Martín.; Lanari, Eduardo.

2003-01-01

373

Influence of yellow rust infection on /sup 32/P transport in detached barley leaves  

Energy Technology Data Exchange (ETDEWEB)

Several barley cultivars (Hordeum vulgare L.) differing in their resistance to yellow rust (Puccinia striiformis West.) were tested for relationships between changes of /sup 32/P transport in detached leaves and resistance to yellow rust disease. Investigation carried out with detached second leaves from plants infected at their first leaf revealed a matter transport in these leaves changed by the infection. Transport was also influenced by inoculation with yellow rust uredospores. In that case rust infection influenced the basipetal transport less strongly in resistent plants than in susceptible ones. Connected with the findings the influence of fungal substances on transport processes is discussed in general.

Schubert, J. (Akademie der Landwirtschaftswissenschaften der DDR, Aschersleben. Inst. fuer Phytopathologie)

1982-01-01

374

Studies on /sup 32/P transport and yellow rust resistance in barley  

Energy Technology Data Exchange (ETDEWEB)

Several cultivars of barley (Hordeum vulgare L.) differing in their resistance to yellow rust were used to study the influence of the infection with Puccinia striiformis West. (strain 24) on /sup 32/P transport in intact plants and isolated leaves. Close correlations exist between transport processes and resistance. For example, resistant plants seem to have a more intensive matter transport than susceptible ones. The importance of the rate of transport to the effectiveness of hypothetic inducers of resistance reactions and defence substances is discussed.

Schubert, J. (Akademie der Landwirtschaftswissenschaften der DDR, Aschersleben. Inst. fuer Phytopathologie)

1982-01-01

375

Implantation of ?-emitters on biomedical implants: 32 P isotropic ion implantation using a coaxial plasma reactor  

International Nuclear Information System (INIS)

The development of endovascular brachytherapy and the treatment of certain types of cancers (liver, lung, prostate) often require the use of beta-emitters, sometimes in the form of radioisotope-implanted devices. Among the most commonly used isotopes figures 32P, a pure beta-emitter (maximum energy: 1.7 MeV), of which the path in biological tissues is of a few cm, restricting the impact of electron bombardment to the immediate environment of the implant. Several techniques and processes have been tried to elaborate surfaces and devices showing strongly bonded, or implanted32P. Anodizing, vapor phase deposition, grafting of oligonucleotides, as well as ion implantation processes have been investigated by several research groups as methods to implant beta-radioisotopes into surfaces. A coaxial plasma reactor was developed at INRS to implant radioisotopes into cylindrical metallic objects, such as coronary stents commonly used in angioplasty procedures. The dispersion of32P atoms on the interior surfaces of the chamber can be investigated using radiographs, contributing to image the plasma ion transport mechanisms that guide the efficiency of the implantation procedure. The amount of radioactivity on the wall liner, on the internal components, and on the biomedical implants are quantified using a surface barrier detector. A comparative study establishes a relationship between the gray scale of the radiographs, and dose measurements. A program was developed to convert the digitized images into maps showing surface dose density in mCi/cm2. An integration process allows the quantification of the doses on the walls and components of the reactor. Finally, the resulting integral of the32P dose is correlated to the initial amount of radioactivity inserted inside the implanter before the dismantling procedure. This method could be introduced as a fast and reliable way to test, qualify and assess the amount of radioactivity present on the as-produced implants. Moreover, the technique can be applied to image and retrace the contamination arising throughout the implantation procedure, for example on instruments and tools

2003-01-01

376

Designing of a liver phantom to quantify 32p radionuclide using gamma camera  

Directory of Open Access Journals (Sweden)

Full Text Available Introduction: 32P is a promising radionuclide for liver cancer because of its energetic beta emissions. The radionuclide microspheres are injected to liver artery. The injected particles must block the capillaries so radionuclide remain close to the tumor and give high dose to the target. The aim of this study was to determine how effective this method is, so we should quantify activity within organs such as liver and lung. So we provided a liver phantom and took planar images with gamma camera from injection of 32P to the liver phantom and assess the validity of static images for quantification of activity in liver. Methods: In order to design the phantom, ICRU was our reference. The phantom consists of a plastic hollow closure with trapezoidal cross section, which contains radionuclide, pure water and Agaroz powder. In this study, ORBITER7500 is used for counting and imaging. The energy windows and system sensitivity for 32P and LEGP collimator were investigated to determine optimum conditions for quantitative imaging. The phantom containing radionuclide was placed in front of camera head then two-dimensional static image and counting was taken. Experiment was designed in three stages: point source in air, cylindrical phantom and liver volumetric phantom in water. Furthermore Monte Carlo simulation with MCNP4C was done in order to calculate sensitivity. Results: In tentative results static imaging for 15%, 20%, 25% and 30% energy windows with energy level of 80 keV, yield the sensitivity value 1.44×10-6, 2.27×10-6, 2.92×10-6 and 3.54×10-6 respectively; measurement errors were 17%. Moreover, comparison between tentative and simulation results for 15%, 20%, 25% and 30% energy windows with 80 keV energy level signified that relative errors were 3%, 7%, 19% and 43% respectively. Conclusion: The best selections were found LEAP collimator and an energy window of 80 keV±20%. When we used these optimal conditions for image acquisition, the estimation of 32P activity in organs and tumors was within 17% of the true activities. These results are acceptable and illustrated that two dimensional static imaging are beneficial for quantification of activity. Furthermore in this method with the use of two dimensional static imaging we can specify value of activity in any desirable ROI  

Maryam Khazaee Moghadam; Seyed Mahmoud Reza Aghamiri; Hosseyn Pourbeygi

2010-01-01

377

The measurement of activity contained in a 32P stainless-steel stent by destructive assay.  

UK PubMed Central (United Kingdom)

Destructive assays of stainless-steel stents containing 32P were performed. Prior to assay, 24 stents were intercompared on a NaI(Tl) well-type crystal. A subset of stents were then digested in a suitable carrier solution by the addition of concentrated hydrofluoric acid. The final solution was measured by the CIEMAT/NIST tritium efficiency tracing method for liquid scintillation counting. A separate experiment was performed which indicated no loss of activity during stent digestion. Expanded (k = 2) uncertainties on stent activity ranged from 1.0% to 2.6%.

Cessna JT

2002-01-01

378

Hematological changes in the house rat after /sup 32/P internal irradiation  

Energy Technology Data Exchange (ETDEWEB)

Male house rats (Rattus rattus) were injected with /sup 32/P at the dose of 1.46 kBq/g body weight. The blood was collected at post-injection intervals of 1/4, 2, 4, 6 and 8 days and various hematological parameters were estimated. An initial decrease in leukocyte count and total plasma protein content (on days 2 to 4) was noted whereas erythrocyte count, hemoglobin percentage and hematocrit value decreased at later intervals only (on days 6 to 8). The plasma cholesterol level registered significant increase on day 2. The possible reasons for these changes have been discussed in the present report.

Kumar, S.; Pareek, B.P.; Gupta, M.L.; Khan, A.S.; Devi, P.U. (Rajasthan Univ., Jaipur (India). Radiation Biology Lab.)

1982-03-01

379

Formation of DNA adducts from oil-derived products analyzed by {sup 32}P-HPLC  

Energy Technology Data Exchange (ETDEWEB)

The aim of this study was to investigate the genotoxic potential of DNA adducts and to compare DNA adduct levels and patterns in petroleum vacuum distillates, coal tar distillate, bitumen fume condensates, and related substances that have a wide range of boiling temperatures. An in vitro assay was used for DNA adduct analysis with human and rat S-9 liver extract metabolic activation followed by {sup 32}P-postlabeling and {sup 32}P-high-performance liquid chromatography ({sup 32}P-HPLC). For petroleum distillates originating from one crude oil there was a correlation between in vitro DNA adduct formation and mutagenic index, which showed an increase with a distillation temperature of 250 C and a peak around a distillation point of approximately 400 C. At higher temperatures, the genotoxicity (DNA adducts and mutagenicity) rapidly declined to very low levels. Different petroleum products showed a more than 100-fold range in DNA adduct formation, with severely hydrotreated base oil and bitumen fume condensates being lowest. Coal tar distillates showed ten times higher levels of DNA adduct formation than the most potent petroleum distillate. A clustered DNA adduct pattern was seen over a wide distillation range after metabolic activation with liver extracts of rat or human origin. These clusters were eluted in a region where alkylated aromatic hydrocarbons could be expected. The DNA adduct patterns were similar for base oil and bitumen fume condensates, whereas coal tar distillates had a wider retention time range of the DNA adducts formed. Reference substances were tested in the same in vitro assay. Two- and three-ringed nonalkylated aromatics were rather low in genotoxicity, but some of the three- to four-ringed alkylated aromatics were very potent inducers of DNA adducts. Compounds with an amino functional group showed a 270-fold higher level of DNA adduct formation than the same structures with a nitro functional group. The most potent DNA adduct inducers of the 16 substances tested were, in increasing order, 9,10-dimethylanthracene, 7,12-dimethylbenz[a]anthracene and 9-vinylanthracene. Metabolic activation with human and rat liver extracts gave rise to the same DNA adduct clusters. When bioactivation with material from different human individuals was used, there was a significant correlation between the CYP 1A1 activity and the capacity to form DNA adducts. This pattern was also confirmed using the CYP 1A1 inhibitor ellipticine. The {sup 32}P-HPLC method was shown to be sensitive and reproducible, and it had the capacity to separate DNA adduct-forming substances when applied to a great variety of petroleum products. (orig.)

Akkineni, L.K.; Zeisig, M.; Baranczewski, P.; Moeller, L. [Karolinska Inst., Stockholm (Sweden). Dept. of Biosciences at Novum; Ekstroem, L.-G. [AB Nynaes Petroleum, Group Competence Centre, Nynaeshamn (Sweden)

2001-01-01

380

Evaluation of different phosphorus sources to the Rumen microorganisms by the radiophosphorus incorporation (32P)  

International Nuclear Information System (INIS)

'In vitro' assays were carried out to evaluate the phosphorus utilization from dicalcium phosphate and rock phosphates from Patos de Minas, Tapira and Finos de Tapira. Rumen samples (500 ml) were collected from a fistulated steer and aliquots were incubated for 4 hours with 0,1 ?Ci 32p (Na2HPO4) in a medium containing the phosphorus sources. After centrifugation, microorganisms were separated and phosphorus incorporation determined. The mean values were 0.137; 0.128; 0.116 and 0.113 mg for Patos, dicalcium phosphate, Tapira and Finos, respectively. There was significant effect of treatment (P

1990-01-01

 
 
 
 
381

Detection of irradiation induced modifications in foodstuff DNA using 32p post-labelling  

International Nuclear Information System (INIS)

[en] DNA post-labelling has been used successfully to detect damage to DNA caused by a range of damaging agents. The assay results in a fingerprint of changes induced in DNA which might, in principle, be useful as a test for the detection of the irradiation of foods. The authors present their DNA extraction and 32p post-labelling methods from chicken or cooked prawn samples and their analysis method (High Performance liquid chromatography). It's hoped that these results could form the basis of a test to detect if foods have been irradiated

1991-01-01

382

32P tracer investigation on phosphorus utilization by soybean in vertisol  

International Nuclear Information System (INIS)

A pot culture experiment was carried out with four levels of phosphorus using 32P tagged superphosphate (viz. 0,40,80 and 120 kg P2O5/ha) and three varieties of soybean namely Punjab-1, JS-2 and Gaurav to study the uptake and utilization of applied fertilizer phosphorus in vertisol of Jabalpur. Dry matter yield, total P uptake, and percent P derived from fertilizer increased significantly with increasing levels of phosphorus. But percent utilization of added P showed a reverse trend, at all the stages of crop growth. Grain and straw yield at harvest increased significantly with increasing P levels in all the three varieties. (author)

1982-01-01

383

Studies on absorption and translocation of phosphorus using radioactive superphosphate (32P) in coffee plants  

International Nuclear Information System (INIS)

Absorption and translocation (downward as well as lateral) of phosphate ions from foliar applied superphosphate solution (pH 6.5, labelled with 32P) were considerably higher in Coffea arabica L. cv.S. 795 than in Coffea canephera Pierre cv.S. 274 plants. The differential absorption and translocation of phosphate to various plant parts and its accumulation in enlarged flowers buds suggest the possibility that the requirement of phosphorus for flower bud enlargement and blossom (anthesis) is higher in arabica than in robusta. (auth.)

1976-01-01

384

In vitro 32P-labelling of viroid RNA for hybridization studies  

International Nuclear Information System (INIS)

[en] A method is described for the in vitro labelling of viroid RNA for use in hybridization studies. The citrus exocortis viroid (approximately 350 nucleotides) is degraded by hot formamide hydrolysis to fragments ranging from small oligonucleotides to near full lengths, and subsequently labelled to high specific activities by enzymatically attaching 32P to the 5'-end of each molecule. The cleavage step leaves 5' hydroxyl groups which allows the polynucleotide kinase to directly label the RNA fragments without prior enzymatic dephosphorylation. The method is simple, requires no special equipment, and provides a radioactive RNA probe sufficient for most types of hybridization studies. (Auth.)

1980-01-01

385

Human folate metabolism using 14C-accelerator mass spectrometry  

Energy Technology Data Exchange (ETDEWEB)

Folate is a water soluble vitamin required for optimal health, growth and development. It occurs naturally in various states of oxidation of the pteridine ring and with varying lengths to its glutamate chain. Folates function as one-carbon donors through methyl transferase catalyzed reactions. Low-folate diets, especially by those with suboptimal methyltransferase activity, are associated with increased risk of neural tube birth defects in children, hyperhomocysteinemic heart disease, and cancer in adults. Rapidly dividing (neoplastic) cells have a high folate need for DNA synthesis. Chemical analogs of folate (antifolates) that interfere with folate metabolism are used as therapeutic agents in cancer treatment. Although much is known about folate chemistry, metabolism of this vitamin in vivo in humans is not well understood. Since folate levels in blood and tissues are very low and methods to measure them are inadequate, the few previous studies that have examined folate metabolism used large doses of radiolabeled folic acid in patients with Hodgkin?s disease and cancer (Butterworth et al. 1969, Krumdieck et al. 1978). A subsequent protocol using deuterated folic acid was also insufficiently sensitive to trace a physiologic folate dose (Stites et al. 1997). Accelerator mass spectrometry (AMS) is an emerging bioanalytical tool that overcomes the limitations of traditional mass spectrometry and of decay counting of long lived radioisotopes (Vogel et al. 1995). AMS can detect attomolar concentrations of 14 C in milligram-sized samples enabling in vivo radiotracer studies in healthy humans. We used AMS to study the metabolism of a physiologic 80 nmol oral dose of 14 C-folic acid (1/6 US RDA) by measuring the 14 C-folate levels in serial plasma, urine and feces samples taken over a 150-day period after dosing a healthy adult volunteer.

Arjomand, A; Bucholz, B A; Clifford, A J; Duecker, S R; Johnson, H; Schneider, P D; Zulim, R A

1999-03-25

386

Ground-state photoneutron reactions in 14C  

International Nuclear Information System (INIS)

Photoneutron time-of-flight spectra from the 14C(?,n0)13C reaction were measured as functions of laboratory angle over the excitation energy region from 10 to 28 MeV. Angular distribution coefficients and differential cross sections were extracted as functions of excitation energy between 10 and 23 MeV. The angle-integrated ground-state cross section indicates that ground state transitions dominate the T/sub less-than/ giant dipole resonance region below 13 MeV, but only contribute about 50% of the strength in the neutron channel in the rest of the giant dipole resonance region. The results support a mechanism of dominant E1 absorption in the energy region from 13 to 23 MeV where an average value of a2 = -0.5 indicates p/sub 1/2/?d/sub 3/2/ single-particle neutron transitions. Angular distribution information suggests that much of a prominent resonance at 11.3 MeV (with an integrated cross section of about 1.03 MeV mb) is due to an M1 transition from the ground state of 14C. If this is the case, there is little fragmentation of the M1 strength in 12C brought about by the presence of valence neutrons. When combined with the observation of the lack of a pygmy E1 resonance below the giant dipole resonance region, these results suggest that a model of 14C as a 12C ''core'' with two valence, weakly coupled, neutrons is inappropriate. Below an excitation energy of about 19 MeV, there is reasonably good quantitative and qualitative agreement between the present data and the results of a recent shell model calculation

1985-01-01

387

Studies on the metabolism of inorganic sulphur by nitrifying bacterium - Nitrobacter agilis using 35S  

International Nuclear Information System (INIS)

In studies on the incorporation of 35S-labelled sulphate into washed cell suspension of Nitrobacter agilis, the bound sulphate, sulphite, sulphide, cysteine, glutathione, homocysteine, methionine and taurine were found in the hydrolysed protein fraction. As in other micro-organisms, sulphate is first activated by ATP in Nitrobacter before it is reduced. The formation of APS and PAPS was also investigated in the present studies. (author)

1973-01-01

388

Response of a metastable superconducting grains suspension to irradiation by 35S decay electrons  

International Nuclear Information System (INIS)

[en] We report a measurement of the energy loss spectrum of electrons in the beta decay of 35S using a prototype detector composed of a suspension of 10-25 ?m diameter tin grains at 2.3 K. Preliminary analyses suggests the capability of achieving sub-eV resolutions in such measurements, and that -contrary to conventional wisdoms - this is possible with both relatively large grains and a distribution of the grain sizes. (orig.)

1993-10-01

389

Response of a metastable superconducting grains suspension to irradiation by [sup 35]S decay electrons  

Energy Technology Data Exchange (ETDEWEB)

We report a measurement of the energy loss spectrum of electrons in the beta decay of [sup 35]S using a prototype detector composed of a suspension of 10-25 [mu]m diameter tin grains at 2.3 K. Preliminary analyses suggests the capability of achieving sub-eV resolutions in such measurements, and that - contrary to conventional wisdoms - this is possible with both relatively large grains and a distribution of the grain sizes. (orig.)

Girard, T.A. (Centro de Fisica Nuclear, Instituto de Fisica e Matematica, Lisboa (Portugal)); Henriques, R.P. (Dept. de Fisica, Universidade de Lisboa (Portugal)); Collar, J.I. (Dept. of Physics and Astronomy, University of South Carolina, Columbia, SC (United States)); Godinho, M. (Dept. de Fisica, Universidade de Lisboa (Portugal) Centro de Fisica Materia Condensada, Instituto de Fisica e Matematica, Lisboa (Portugal)); Bonfait, G. (ICEN/LNETI, Sacavem (Portugal)); Waysand, G. (Groupe de Physique des Solides, Universites de Paris 7/6, 75 (France)); Limagne, D. (Groupe de Physique des Solides, Universites de Paris 7/6, 75 (France)); Jeudy, V. (Groupe de Physique des Solides, Universites de Paris 7/6, 75 (France)); Pagesy, V. (Groupe de Physique des Solides, Universites de Paris 7/6, 75 (France))

1993-10-01

390

t-(/sup 35/S)butylbicyclophosphorothionate binding sites in invertebrate tissues  

Energy Technology Data Exchange (ETDEWEB)

Specific high affinity binding of the cage convulsant t-(35S)butylbicyclophosphorothionate (TBPS) was observed in membrane homogenates of housefly heads and crayfish abdominal muscles. (35S)TBPS binding in these two invertebrate tissues was inhibited by biologically active cage convulsants, picrotoxin analogs, and barbiturates. The housefly binding sites were inhibited most potently by several insecticides. Approximately 50% of total binding was displaceable by excess (0.1 mM) nonradioactive TBPS, picrotoxinin, ethyl bicyclophosphate, or dieldrin. Optimal binding assay conditions for housefly homogenates included pH 7.5, 22 degrees C temperature, 0.3 M chloride concentration, and incubation for 60 min; for crayfish homogenates, 4 degrees C temperature and 150-min incubations were optimal. Scatchard plots of equilibrium binding indicated one site in both tissues (KD = 50 nM, Bmax = 250 fmol/mg protein in housefly; KD = 25 nM, Bmax = 100 fmol/mg protein in crayfish). Association kinetics in housefly were consistent with one rate constant (k+1 = 8 X 10(6) M-1 min-1), but dissociation was described better by two rate constants (k-1 = 0.28 min-1 and 0.042 min-1; calculated KD values of 80 nM and 12 nM). Displacement by cage convulsants showed Hill numbers near 0.5, also consistent with two populations of affinity, while displacement by other drugs showed Hill numbers near 1.0. (35S)TBPS binding in insects was most potently inhibited by the insecticides dieldrin (IC50 = 50 nM), aldrin, and lindane (200 nM), in a stereospecific manner, consistent with this binding site being the receptor for biological toxicity. (35S)TBPS binding was also inhibited by relatively high concentrations of some pyrethroid insecticides, such as deltamethrin and cypermethrin (1-2 microM).

Olsen, R.W.; Szamraj, O.; Miller, T.

1989-04-01

391

First order /sup 14/C dating of Holocene molluscs  

Energy Technology Data Exchange (ETDEWEB)

The investigation of many problems in geology requires radiometric ages which need not be precise provided they are reliable and preferably cheap and quick. Such first-order ages can be determined on Holocene molluscs at 1/100 the cost of conventional /sup 14/C ages and in the space of a few hours by trapping CO/sub 2/ from the shell carbonate in an organic base for assay in a liquid scintillation counter. Using standard laboratory glassware and a counter with preset channels the technique gives ages for the last 8000 years with a standard error (2 sigma) of 1500 years or less.

Vita-Finzi, C. (University Coll., London (UK))

1983-11-01

392

Studies on 14C labelled chlorpyrifos in model marine ecosystem  

International Nuclear Information System (INIS)

Chlorpyrifos is one of the widely used organophosphorus insecticides in tropical countries. Experiments were conducted with 14C labelled chlorpyrifos to study the distribution of this compound in model marine ecosystem. Less than 50 per cent of the applied activity remained in water in 24 h. Major portion of the applied chlorpyrifos (about 4.2 % residue per g) accumulated into the clams with sediment containing a maximum of 5 to 6 per cent of applied compound. No degradation of chlorpyrifos was observed in water or sediment samples. However, metabolic products were formed in clams. (author). 4 refs., 3 tabs

1997-01-01

393

Radiative neutron capture on 14C and 14N  

CERN Multimedia

The possibility of description of the experimental data on total cross sections of the radiative neutron capture on 14C and 14N is considered within the frame of the potential cluster model with forbidden states and their classification according to Young schemes. It is shown that the using model and the potential construction methods allow to reproduce correctly the behavior of experimental cross sections at the energies from 10 meV (10-2 eV) to 1 MeV.

Afanasyeva, N V; Dzhazairov-Kakhramanov, A V

2012-01-01

394

Chemical radiolabeling of carboxyatractyloside by [14C]acetic anhydride  

International Nuclear Information System (INIS)

[en] The authors report the synthesis and biological properties of a radiolabeled derivative of CAT obtained with acetylation of the primary alcohol of CAT with radiolabeled acetic anhydride. They also investigate the question of mutual exclusion of CAT and BA for binding to the mitochondrial ADP/ATP carrier in double labeling experiments based on the use of [3H]BA and [14C]Ac-CAT. The results are consistent with the view that the ADP/ATP carrier possesses two separate interacting binding sites for AT (or CAT) and for BA. (Auth.)

1980-08-11

395

14C-lindane residues in palm processing  

International Nuclear Information System (INIS)

A study was undertaken to identify the residue level in palm fruits and its products by spiking the fruits at 5 ppm with 14C-[UL]-lindane and following up on the residue during simulated commercial processes. The study concluded that steam distillation (deodorization) is an effective process in removing lindane residues. Under experimental conditions, commercial processes removed some 74% of insecticide residues from crude palm oil. Based on the oil content (up to 50%) of the pulp the residue concentration in the refined oil may exceed 2.5% mg/kg. The result suggest that the refined oil may retain one quarter of lindane received by the fruit. 1 tab.

1994-02-04

396

Combustion synthesis of ceramic matrices for immobilization of 14C  

International Nuclear Information System (INIS)

In this study, the use of combustion synthesis for immobilization of 14C was considered. Ceramic matrices have been prepared by this method using two different devices: one non-conventional with preheating of the samples and the other conventional device where ignition was produced thanks to tungsten filament. These two devices gave rise to different mechanisms of reactions involving different amounts of unreacted carbon graphite inside the matrix. The SHS samples were characterized by using scanning electron microscopy (SEM) and X-ray diffraction (XRD)

2008-10-20

397

Effect of diabetes on in vivo metabolism of [35S]-labeled glomerular basement membrane  

International Nuclear Information System (INIS)

Glomerular basement membrane (GBM) was labeled in vivo by the injection of tracer amounts of [35S]-sulfate into normal and streptozotocin-diabetic rats. The biosynthesis and turnover of sulfated glycosaminoglycans in the GBM was determined from the specific activity of [35S] after pronase digestion of basement membranes purified from glomeruli isolated 1-7 days after injection. Peak radiolabeling of both normal and diabetic GBM occurred 24 h after injection and, when corrected for differences in serum sulfate specific activities, was less in diabetic than in normal samples. The specific activity of GBM sulfate, expressed as cpm/microgram uronic acid, progressively diminished over the ensuing period of study in both normal and diabetic samples. The rate of decrease in specific activity of [35S]-labeled GBM was not significantly different in diabetic preparations compared with that in normal controls. The findings are compatible with diminished sulfation and/or production but normal turnover of glycosaminoglycans in the renal GBM in experimental diabetes.

1984-01-01

398

Effect of diabetes on in vivo metabolism of (/sup 35/S)-labeled glomerular basement membrane  

Energy Technology Data Exchange (ETDEWEB)

Glomerular basement membrane (GBM) was labeled in vivo by the injection of tracer amounts of (/sup 35/S)-sulfate into normal and streptozotocin-diabetic rats. The biosynthesis and turnover of sulfated glycosaminoglycans in the GBM was determined from the specific activity of (/sup 35/S) after pronase digestion of basement membranes purified from glomeruli isolated 1-7 days after injection. Peak radiolabeling of both normal and diabetic GBM occurred 24 h after injection and, when corrected for differences in serum sulfate specific activities, was less in diabetic than in normal samples. The specific activity of GBM sulfate, expressed as cpm/microgram uronic acid, progressively diminished over the ensuing period of study in both normal and diabetic samples. The rate of decrease in specific activity of (/sup 35/S)-labeled GBM was not significantly different in diabetic preparations compared with that in normal controls. The findings are compatible with diminished sulfation and/or production but normal turnover of glycosaminoglycans in the renal GBM in experimental diabetes.

Cohen, M.P.; Surma, M.L.

1984-01-01

399

Comparison of the mutagenic effects of 35S decay in Escherichia coli strains WP-2 and WP-2S.  

UK PubMed Central (United Kingdom)

Comparison was made of the lethal and mutagenic efficiency of 35S yields 35Cl transmutation of incorporated 35S in cells of Escherichia coli strain WP-2 and WP-2S (UV-sensitive). Bacteria were stored at minus 196 degrees. 35S yields 35Cl transmutation induced a higher lethal effect in strain WP-2 than in the UV-sensitive strain WP-2S. Reversions try yields try+ were induced with an approximately similar efficiency in both strains compared.

Pluciennik H; Ka?ski R

1975-01-01

400

Dissipation of 14C chlorpyrifos in the rhizosphere of rice  

International Nuclear Information System (INIS)

The root exudates from the plants contribute to the biodegradation of insecticides. Although, different mechanisms have been proposed, there is no clear elucidation of any mechanism. This study investigates the dissipation of an organophosphorus insecticide, chlorpyrifos in the rhizospheric soil planted with rice plant. Two sets of experimental tanks were maintained with or without plants using soil spiked with 1 mg kg-1 and 10 mg kg-1 of chlorpyrifos. Experiment was conducted for 180 days till the rice plant starts bearing seeds. The 14C activity decreased rapidly in the rhizospheric soil as compare to the non-rhizospheric soil. The total culturable microflora were higher in the rhizospheric than the non-rhizospheric soil. The plant extract had given few counts indicating some negligible amount of chlorpyrifos uptake. The 14C activity in the water was disappeared after 30 days. It was observed that very low amount of residue persisted in soil. This studies revealed that the plants play an important role in the dissipation of the chlorpyrifos from the rice flooded rhizospheric soil. (author)

2004-01-01

 
 
 
 
401

Structure in the 14C+16O reaction cross sections  

International Nuclear Information System (INIS)

[en] The yields of several nuclides produced in the 14C+16O reaction have been measured over the range E/sub c.m./=16--34 MeV. The excitation function for the production of 22Ne displays a regular sequence of narrow fine-structure resonances similar to those previously observed in the 12C+16O reaction. This is the first time that such structure has been observed for systems of non-?-particle nuclei. Intermediate-width resonances are observed in the yield of 18O at E/sub c.m./=19.5, 23.5, 27.5, and 31 MeV. Correlated structure appears in the inelastic scattering cross section to the 3- state of 14C at all these energies, except possibly E/sub c.m./=19.5 MeV. The resemblance of the 23.5-MeV structure to anomalies previously observed in the 16O+16O and 12C+16O reactions is noted, and some properties of the compound system are deduced

1981-01-01

402

Ground-state photoneutron reactions in /sup 14/C  

Energy Technology Data Exchange (ETDEWEB)

Photoneutron time-of-flight spectra from the /sup 14/C(..gamma..,n/sub 0/)/sup 13/C reaction were measured as functions of laboratory angle over the excitation energy region from 10 to 28 MeV. Angular distribution coefficients and differential cross sections were extracted as functions of excitation energy between 10 and 23 MeV. The angle-integrated ground-state cross section indicates that ground state transitions dominate the T/sub less-than/ giant dipole resonance region below 13 MeV, but only contribute about 50% of the strength in the neutron channel in the rest of the giant dipole resonance region. The results support a mechanism of dominant E1 absorption in the energy region from 13 to 23 MeV where an average value of a/sub 2/ = -0.5 indicates p/sub 1/2/..-->..d/sub 3/2/ single-particle neutron transitions. Angular distribution information suggests that much of a prominent resonance at 11.3 MeV (with an integrated cross section of about 1.03 MeV mb) is due to an M1 transition from the ground state of /sup 14/C. If this is the case, there is little fragmentation of the M1 strength i

Kuo, P.C.; McNeill, K.G.; Sherman, N.K.; Landsberger, S.; Davidson, W.F.; Jury, J.W.; Lafontaine, J.R.C.

1985-02-01

403

(32)P colloid radiosynovectomy in treatment of chronic haemophilic synovitis: Iran experience.  

Science.gov (United States)

Repeated intra-articular bleeding with subsequent development of chronic synovitis and cartilage changes, leading to haemophilic arthropathy, is one the most debilitating problems in haemophilic patients. Radiosynovectomy is a familiar therapeutic choice in management of chronic synovitis in haemophilia. We report the treatments results of synoviorthesis with (32)P chromic phosphate with emphasis on clinical aspects. Between 2002 and 2006 we performed 66 procedures in 53 patients. Seven patients were excluded. The remaining 46 patients were followed for an average of 31 months. The mean age of patients at the time of injection was 15.9 years (range: 6-28). There were three repeat injections. According to Fernandez-pallazi and Cavilgia clinical classification (Table 1) [23], nine joints were Stage II and 46 were Stage III. In latest follow-up, 77% of patients reported at least a 50% decrease in bleeding frequency after treatment (P < 0.0001). The need for antihaemophilic factor consumption dropped by about 74% postradiosynovectomy (P < 0.0001). In most of the injected joints, the range of motion remained stable or improved. A trend was found for the number of haemarthrosis to increase after a period of considerable improvement. Synoviorthesis using (32)P effectively reduces the intra-articular bleeding rate and factor concentrate use. Durability of the response seems to be unpredictable, perhaps attributable to the late intervention. An early radiosynovectomy might be more helpful in terms of stability of response to treatment. PMID:17286772

Mortazavi, S M J; Asadollahi, S; Farzan, M; Shahriaran, S; Aghili, M; Izadyar, S; Lak, M

2007-03-01

404

(32)P colloid radiosynovectomy in treatment of chronic haemophilic synovitis: Iran experience.  

UK PubMed Central (United Kingdom)

Repeated intra-articular bleeding with subsequent development of chronic synovitis and cartilage changes, leading to haemophilic arthropathy, is one the most debilitating problems in haemophilic patients. Radiosynovectomy is a familiar therapeutic choice in management of chronic synovitis in haemophilia. We report the treatments results of synoviorthesis with (32)P chromic phosphate with emphasis on clinical aspects. Between 2002 and 2006 we performed 66 procedures in 53 patients. Seven patients were excluded. The remaining 46 patients were followed for an average of 31 months. The mean age of patients at the time of injection was 15.9 years (range: 6-28). There were three repeat injections. According to Fernandez-pallazi and Cavilgia clinical classification (Table 1) [23], nine joints were Stage II and 46 were Stage III. In latest follow-up, 77% of patients reported at least a 50% decrease in bleeding frequency after treatment (P < 0.0001). The need for antihaemophilic factor consumption dropped by about 74% postradiosynovectomy (P < 0.0001). In most of the injected joints, the range of motion remained stable or improved. A trend was found for the number of haemarthrosis to increase after a period of considerable improvement. Synoviorthesis using (32)P effectively reduces the intra-articular bleeding rate and factor concentrate use. Durability of the response seems to be unpredictable, perhaps attributable to the late intervention. An early radiosynovectomy might be more helpful in terms of stability of response to treatment.

Mortazavi SM; Asadollahi S; Farzan M; Shahriaran S; Aghili M; Izadyar S; Lak M

2007-03-01

405

Highly efficient absorbents for removing 32P-labeled nucleotides from experimental waste  

International Nuclear Information System (INIS)

[en] Three kinds of charcoal (LH2C20/48, WH5C8/32 and X-7100-3), three kinds of ion exchange resin (DE52, MB5113J and RG5O1/X8), and one metal chelatar (MC75) were tested for their ability to remove (?-32P) dCTP as a standard RI using a small column system. The most efficient absorber was DE52. However, DE52 was not suitable for practical treatment of the waste because flow rate was slow. LH2C20/48, WH5C8/32 and MB5113J also showed high absorption ability and their absorption efficiency was not affected by a pH range from 5 to 9 of the solution. On the basis of this result, a practical trial of the radioisotope waste treatment was also made. It was concluded that among the tested absorbents MB5113J was the most applicable for removing 32P-labeled nucleotides from experimental waste. (author)

1997-01-01

406

Highly efficient absorbents for removing {sup 32}P-labeled nucleotides from experimental waste  

Energy Technology Data Exchange (ETDEWEB)

Three kinds of charcoal (LH2C20/48, WH5C8/32 and X-7100-3), three kinds of ion exchange resin (DE52, MB5113J and RG5O1/X8), and one metal chelatar (MC75) were tested for their ability to remove ({alpha}-{sup 32}P) dCTP as a standard RI using a small column system. The most efficient absorber was DE52. However, DE52 was not suitable for practical treatment of the waste because flow rate was slow. LH2C20/48, WH5C8/32 and MB5113J also showed high absorption ability and their absorption efficiency was not affected by a pH range from 5 to 9 of the solution. On the basis of this result, a practical trial of the radioisotope waste treatment was also made. It was concluded that among the tested absorbents MB5113J was the most applicable for removing {sup 32}P-labeled nucleotides from experimental waste. (author)

Nakano, Miyoko; Iwatani, Kazuo; Kura, Hideto; Kohchi, Chie; Yamamoto, Osamu [Hiroshima Univ. (Japan). Radioisotope Center

1997-09-01

407

Histological study of the early stage of 32P-induced experimental osteosarcoma  

International Nuclear Information System (INIS)

32P radioisotope as an orthophosphate solution was injected intraperitoneally into C.F. Wistar strain albino rats to induce primary osteosarcoma. To capture the early stage of tumor formation, bone scintigraphy employing technetium-99m ethane-1-hydroxy-1,1-diphosphonate and soft radiography were conducted from week 16 after the beginning of 32P administration. The histological findings were compare