A brief description of the morphological and chemical structure of Li po polysaccharides is given, as well as its occurrence in nature and its mechanisms of action. It is emphasized the usefulness for actual biochemical and biomedical research of the labeled Lipopolysaccharide. The method for the labelling, isolation and purification of 14''C-Lipopolysacchari de is described. (Author) 23 refs
Preparation of a Lipopolysaccharide from Escherichia coli 01lla, 01llb, k58: h21 bacterial wall, labeled with carbon-14; Preparacion de un lipopolisacarido de la pared baceteriana de escherichia coli 01lla, 01llb, K58: H21, marcado con carbono-14
Solano Aunon, M. L.; Pacheco Lopez, J.; Garcia Pineda, M. D.; Roca, M.; Bayon, A.
A brief description of the morphological and chemical structure of Li po polysaccharides is given, as well as its occurrence in nature and its mechanisms of action. It is emphasized the usefulness for actual biochemical and biomedical research of the labeled Lipopolysaccharide. The method for the labelling, isolation and purification of 14''C-Lipopolysacchari de is described. (Author) 23 refs.
Heegaard, N.H.H.; Jørgensen, T.J.D.; Rozlosnik, N.;
. Using amide hydrogen/deuterium exchange monitored by mass spectrometry, we show that Delta K58-beta(2)m has increased unfolding rates compared to wt-beta(2)m and that unfolding is highly temperature dependent. The unfolding rate is I order of magnitude faster in Delta K58-beta(2)M than in wt-beta(2)m...... fluorescence. After a few days at 37 degrees C, in contrast to wt-beta(2)M, Delta K-58-beta(2)M forms well-defined high molecular weight aggregates that are detected by size-exclusion chromatography. Atomic force microscopy after seeding with amyloid-beta(2)m fibrils under conditions that induce minimal...
A. Deinert; A.C. Homan; D. Boer; S.C. Voelpel; D. Gutermann
The multi-dimensionality of the transformational leadership construct has been under debate in the last decades. To shed more light on this issue, we conducted a meta-analysis (k = 58 studies), examining the transformational leadership sub-dimensions and their links to leader personality and perform
Hofstra, H.; Van Tol, J D; Dankert, J
Outer membrane fractions were prepared from 11 bacteria in the family Enterobacteriaceae: Escherichia coli serotypes O1K-, O4K2, O26K60, O75K-, and O111K58, Shigella flexneri, Salmonella typhimurium, Klebsiella pneumonia, Serratia marcescens, Proteus vulgaris, Proteus mirabilis, and Providencia stuartii. All strains studied were found to contain one non-peptidoglycan-bound, heat-modifiable outer membrane protein, and one or two peptidoglycan-associated major outer membrane proteins in the 27,...
Bridge, John H. B.; Bersohn, Malcolm M.; Gonzalez, Frank; BASSINGTHWAIGHTE, JAMES B.
A new γ-labeled marker for extracellular space is the cobaltic form of 58Co-ethylenediaminetetraacetic acid (58Co-EDTA). The cobaltic ion has a much higher affinity for EDTA than the cobaltous ion; it is prepared as a potassium salt, K+(58Co3+-EDTA4−), and is apparently biologically inert. Testing by equilibration in intact rabbits and comparing the myocardial content with that of [14C]sucrose give values of the volume of distribution in the myocardium of 0.294 ± 0.052 ml/g for 58Co-EDTA and ...
Cevolani, G.; Dardi, A.; Formiggini, C. (Consiglio Nazionale delle Ricerche, Bologna (Italy). Ist. FISBAT); Kingsley, S.P.; Muller, H.G. (Sheffield Univ. (UK). Dept. of Physics)
Simultaneous meteor radar wind observations were made at Budrio (Italy, 45/sup 0/ N), Sheffield (U.K., 53/sup 0/ N) and Stornoway (U.K., 58/sup 0/ N) during the Energy Budget Campaign (EBC), November-December 1980, in the altitude region between 75 and 115 km. Wind results are presented in terms of prevailing components and oscillations of different time scales: Tidal (8, 12 and 24 h), gravity ((3/6) h) and planetary ((2/10) days) waves. The seasonal variation of the prevailing zonal components observed at the three stations in previous years shows the same maxima of Summer and Winter, as well as the equinoctial minima exhibited by the CIRA 1972 model for 50/sup 0/ N. The data show marked tidal activity with the semi-diurnal tide which exceeds in amplitude any other mode in the wind spectra.
WANG Jiasheng; ZHANG Wei; CHENG Ying; KE Dandan; HAN Shumin
The hydrogenation/dehydrogenation kinetics and thermodynamic behaviors of the MgH2-WS2 composites were investigated. The TPD (Temperature-Programmed-Desorption) curves showed that the onset dehydrogenation temperature of the MgH2 + 20wt% WS2 composite was 615 K, 58 K lower than that of the pristine MgH2. The kinetic measurements showed that within 21 min, the MgH2 + 20wt% WS2 composite could absorb 2.818wt% at 423 K, and release 4.244 wt% of hydrogen at 623 K, while the hydriding/dehydriding capacity of MgH2 reached only 0.979wt% and 2.319wt% respectively under identical conditions. The improvement of hydrogenation/dehydrogenation performances for the composite was attributed to the co-catalytic effect between the new phases W and MgS which formed during the ball-milling process.
Janković, Marija R.; Dmitrašinović, V.
We use 57 recently found topological satellites of Broucke-Hadjidemetriou-Hénon's periodic orbits with values of the topological exponent k ranging from k =3 to k =58 to plot the angular momentum L as a function of the period T , with both L and T rescaled to energy E =-0.5 . Upon plotting L (T /k ) we find that all our solutions fall on a curve that is virtually indiscernible by the naked eye from the L (T ) curve for nonsatellite solutions. The standard deviation of the satellite data from the sixth-order polynomial fit to the progenitor data is σ =0.13 . This regularity supports Hénon's 1976 conjecture that the linearly stable Broucke-Hadjidemetriou-Hénon orbits are also perpetually, or Kol'mogorov-Arnol'd-Moser, stable.
Janković, Marija R
We use 57 recently found topological satellites of Broucke-Hadjidemetriou-Henon's periodic orbits with values of the topological exponent $k$ ranging from $k$ = 3 to $k$ = 58 to plot the angular momentum $L$ as a function of the period $T$, with both $L$ and $T$ rescaled to energy $E=-\\frac12$. Upon plotting $L(T/k)$ we find that all our solutions fall on a curve that is virtually indiscernible by naked eye from the $L(T)$ curve for non-satellite solutions. The standard deviation of the satellite data from the sixth-order polynomial fit to the progenitor data is $\\sigma = 0.13$. This regularity supports Henon's 1976 conjecture that the linearly stable Broucke-Hadjidemetriou-Henon orbits are also perpetually, or Kolmogorov-Arnold-Moser stable.
Small-scale (laboratories and hospitals) to global radioactive contamination may contribute to incorporation of radionuclides in the living body. In real accidents, many radionuclides are occasionally incorporated simultaneously into the body. Therefore, it is important to know the kinds of radionuclides, chemical form at the incorporation, and presence status and behavior of radionuclides in the body. Because there is interaction between agents for reducing radioactive contamination, the reduction mechanism of radionuclide in the body should be considered when such agents are used in the combination manner. The purpose of this report is to discuss chemical methods for reducing radionuclides that are orally incorporated into the body. These methods are described in terms of dilution, complex production, adsorption, metabolism derangement, and others. I-131, Cs-137, Sr-90, and Ru-106, which are influential radionuclides for the human body, are especially referred to. (N.K.) 58 refs
Pennington, Lindsay; Virella, Daniel; Mjøen, Tone; da Graça Andrada, Maria; Murray, Janice; Colver, Allan; Himmelmann, Kate; Rackauskaite, Gija; Greitane, Andra; Prasauskiene, Audrone; Andersen, Guro; de la Cruz, Javier
Surveillance registers monitor the prevalence of cerebral palsy and the severity of resulting impairments across time and place. The motor disorders of cerebral palsy can affect children's speech production and limit their intelligibility. We describe the development of a scale to classify children's speech performance for use in cerebral palsy surveillance registers, and its reliability across raters and across time. Speech and language therapists, other healthcare professionals and parents classified the speech of 139 children with cerebral palsy (85 boys, 54 girls; mean age 6.03 years, SD 1.09) from observation and previous knowledge of the children. Another group of health professionals rated children's speech from information in their medical notes. With the exception of parents, raters reclassified children's speech at least four weeks after their initial classification. Raters were asked to rate how easy the scale was to use and how well the scale described the child's speech production using Likert scales. Inter-rater reliability was moderate to substantial (k>.58 for all comparisons). Test-retest reliability was substantial to almost perfect for all groups (k>.68). Over 74% of raters found the scale easy or very easy to use; 66% of parents and over 70% of health care professionals judged the scale to describe children's speech well or very well. We conclude that the Viking Speech Scale is a reliable tool to describe the speech performance of children with cerebral palsy, which can be applied through direct observation of children or through case note review. PMID:23891732
Thiago Batista Firmato de Almeida
Full Text Available Lettuce is the most popular of the leafy vegetables. It is known worldwide and its consumption occurs mainly in the natural form. The objective was to evaluate the effect of macronutrient omission on the growth and nutritional status of the lettuce cv. Veronica, and to describe the visual symptoms of nutritional deficiency. The treatments complete consisted of the solution and the individual omission of N, P, K, Ca, Mg and S, under a completely randomized design with three replications. Plants were grown in Hoagland & Arnon, in pots (8L. At 56 days after transplant we evaluated plant height, leaf number, leaf area, SPAD index, dry matter of shoots, roots and whole plant, levels of macronutrients in shoots and roots, and the nature of nutritional disorders. The omission of nutrients affected the growth variables. The nutrients found in lettuce plants from nutrient solution and the complete omission in the shoot were, respectively, N= 23.2 to 9.5, P= 5.4 to 1.3, K= 58,9 to 3.2, Ca= 12.1 to 3.6, Mg= 5.5 to 0.7 and S= 3.2 to 1.5g.kg-1. The omission of macronutrients caused quality losses, since it affected the nutrition of the vegetables and this resulted in morphological changes, reflected as symptoms of deficiency for each nutrient.
Fokina, Oleksandra; Chellamuthu, Vasuki-Ranjani; Forchhammer, Karl; Zeth, Kornelius
P(II) proteins control key processes of nitrogen metabolism in bacteria, archaea, and plants in response to the central metabolites ATP, ADP, and 2-oxoglutarate (2-OG), signaling cellular energy and carbon and nitrogen abundance. This metabolic information is integrated by P(II) and transmitted to regulatory targets (key enzymes, transporters, and transcription factors), modulating their activity. In oxygenic phototrophs, the controlling enzyme of arginine synthesis, N-acetyl-glutamate kinase (NAGK), is a major P(II) target, whose activity responds to 2-OG via P(II). Here we show structures of the Synechococcus elongatus P(II) protein in complex with ATP, Mg(2+), and 2-OG, which clarify how 2-OG affects P(II)-NAGK interaction. P(II) trimers with all three sites fully occupied were obtained as well as structures with one or two 2-OG molecules per P(II) trimer. These structures identify the site of 2-OG located in the vicinity between the subunit clefts and the base of the T loop. The 2-OG is bound to a Mg(2+) ion, which is coordinated by three phosphates of ATP, and by ionic interactions with the highly conserved residues K58 and Q39 together with B- and T-loop backbone interactions. These interactions impose a unique T-loop conformation that affects the interactions with the P(II) target. Structures of P(II) trimers with one or two bound 2-OG molecules reveal the basis for anticooperative 2-OG binding and shed light on the intersubunit signaling mechanism by which P(II) senses effectors in a wide range of concentrations. PMID:21041661
Surendran, U; Rama Subramoniam, S; Raja, P; Kumar, V; Murugappan, V
Mining of nutrients from soil is a major problem in developing countries causing soil degradation and threaten long-term food production. The present study attempts to apply NUTrient MONitoring (NUTMON) model for carrying out nutrient budgeting to assess the stocks and flows of nitrogen (N), phosphorus (P), and potassium (K) in defined geographical unit based on the inputs, viz., mineral fertilizers, manures, atmospheric deposition, and sedimentation, and outputs, viz., harvested crop produces, residues, leaching, denitrification, and erosion losses. The study area covers Coimbatore and Erode Districts, which are potential agricultural areas in western agro-ecological zone of Tamil Nadu, India. The calculated nutrient balances for both the districts at district scale, using NUTMON methodology, were negative for nitrogen (N -3.3 and -10.1 kg ha(-1)) and potassium (K -58.6 and -9.8 kg ha(-1)) and positive for phosphorus (P +14.5 and 20.5 kg ha(-1)). Soil nutrient pool has to adjust the negative balance of N and K; there will be an expected mining of nutrient from the soil reserve. A strategy was attempted for deriving the fertilizer recommendation using Decision Support System for Integrated Fertilizer Recommendation (DSSIFER) to offset the mining in selected farms. The results showed that when DSSIFER recommended fertilizers are applied to crops, the nutrient balance was positive. NUTMON-Toolbox with DSSIFER would serve the purpose on enhancing soil fertility, productivity, and sustainability. The management options to mitigate nutrient mining with an integrated system approach are also discussed. PMID:27021693
Deng, Bowen; Chen, Zhigang; Gao, Muxing; Song, Yuqiao; Zheng, Kaiyuan; Tang, Juanjuan; Xiao, Wei; Mao, Xuhui; Wang, Dihua
Electrochemical transformation of CO2 into functional materials or fuels (i.e., carbon, CO) in high temperature molten salts has been demonstrated as a promising way of carbon capture, utilisation and storage (CCUS) in recent years. In a view of continuous operation, the electrolysis process should match very well with the CO2 absorption kinetics. At the same time, in consideration of the energy efficiency, a molten salt electrochemical cell running at lower temperature is more beneficial to a process powered by the fluctuating renewable electricity from solar/wind farms. Ternary carbonates (Li : Na : K = 43.5 : 31.5 : 25.0) and binary chlorides (Li : K = 58.5 : 41.5), two typical kinds of eutectic melt with low melting points and a wide electrochemical potential window, could be the ideal supporting electrolyte for the molten salt CO2 capture and electro-transformation (MSCC-ET) process. In this work, the CO2 absorption behaviour in Li2O/CaO containing carbonates and chlorides were investigated on a home-made gas absorption testing system. The electrode processes as well as the morphology and properties of carbon obtained in different salts are compared to each other. It was found that the composition of molten salts significantly affects the absorption of CO2, electrode processes and performance of the product. Furthermore, the relationship between the absorption and electro-transformation kinetics are discussed based on the findings. PMID:27193751
Torger, B.; Müller, M.
In-situ ATR-FTIR spectroscopy and line shape analysis of the diagnostic spectral region was used to quantify the bound amount and release of the antibiotic streptomycin (STRP) at polyelectrolyte (PEL) multilayers (PEM) of poly(ethyleneimine) (PEI) and poly(acrylic acid) (PAA) or PEI and sodium alginate (ALG). Unlike common concepts based on the drug enrichment of the release medium, this analytical concept allowed to measure quantitatively the drug depletion in the delivery matrix. The measured kinetic in situ ATR-FTIR data were analysed by a modified Korsmeyer-Peppas equation based on two characteristic release parameters k and n. As main experimental parameters the number of PEL layers (adsorption steps) z and the STRP/PEL ratio were varied. For z = 8 the STRP/PEL ratio showed the most significant influence on release kinetics, whereby for STRP/PEL = 1:25 slowest (n = 0.77) and lowest (k = 21.4%) and for STRP/PEL = 1:5 most rapid (n = 0.30) and highest (k = 58.6%) drug releases were found. PEM-PEI/ALG-8 (STRP/PEL = 1:5) revealed slower release rates (n = 0.58) and lower released STRP amounts (k = 17.1%) compared to PEI/PAA. UV-VIS data on time dependent STRP enrichment of the release medium showed a similar trend compared to respective ATR-FTIR data on STRP depletion in PEM. Released amounts of around 1-2 mg from the herein introduced PEM films could be determined. The introduced analytical concept will be used as screening tool for other drugs, drug eluting films and bone substituting materials.
Cevolani, G.; Dardi, A.; Formiggini, C. (Consiglio Nazionale delle Ricerche, Bologna (Italy)); Kingsley, S.P.; Muller, H.G. (Sheffield Univ. (UK). Dept. of Physics)
Simultaneous meteor radar wind observations were made at Budrio (Italy,45 deg N), Sheffield (U.K.,53 deg N) and Stornoway (U.K.,58 deg N) during the Energy Budget Campaign (EBC), November-December 1980, in the altitude region between 75 and 115 km. Wind results are presented in terms of prevailing components an oscillations of different time scales: tidal (8,12 and 24 h), gravity ((3/6)h) and planetary ((2/10) days) waves. The seasonal variation of the prevailing zonal components observed at the three stations in previous years shows the same maxima of Summer and Winter, as well as the equinactial minima exhibited by the CIRA 19972 model for 50 deg N. The data show marked tidal activity with the semi-diurnal tide which exceeds in amplitude any other mode in the wind spectra. Tidal amplitudes recorded at Budrio appear more significantly lower than those recorded at Sheffield and Stornoway. Variations of the amplitude of maxima of the semi-diurnal oscillation relative to the Budrio and Sheffield data show time scales generally greater than 5 days throughout the recording period. The diurnal and terdiurnal tides represent a small part of the total wind energy in the meteor zone and exhibit phases which disagree strongly at the quoted stations. The scatter in the hourly data is representative of small-scale wind disturbances due to internal gravity waves: at Budrio, by using the MEM method for individual day's data (November 23-24, 28-29, 1980), gravity waves with vertical wave-lengths of (20/45) km, periods of 3.5, 4.5 and 6 h, and amplitudes of the order of 10 ms/sup -1/ have been observed. Long-period oscillations with periods in the (2/10) day time interval have been identified in the wind patterns at Budrio and Sheffield, but the presence of planetary waves of quasi-2-day period was less significant than during a previous Summer 1980 run carried out at the same stations.
Patil, S. K.; Rao, D. R. K.
Detailed alternating field (AF) and thermal demagnetization techniques on 120 samples belonging to 10 dykes of the Goa region along the west coast of the Indian Precambrian Shield, have yielded characteristic remanent magnetization (ChRM) directions from 9 dykes of which 6 dykes exhibited a reverse magnetic polarity direction ( D=150°, I=44.30°, k=58, α95=5.8, N=6 dykes), while 3 dykes exhibited normal polarity D=351°, I=-50°, k=21, α95=14.9, N=3 dykes) direction. The remaining 1 dyke has shown stable direction, but with a strong overprint of the present earth field (PEF) direction, which could not be removed by either AF or thermal cleaning techniques. A Palaeomagnetic pole, computed by averaging the VGPs of 9 dykes is situated at 41.2°N 78.1°W ( N=9 dykes; A95=9.81°), which is close to that of the Deccan super pole (36.9°N, 78.8°W), suggesting that the dykes in the Goa region are related to the Deccan basaltic eruptions. Recently published 40Ar/ 39Ar dating on the normal polarity dykes of the studied area have assigned the ages as 62.8±0.2 Ma. These results demonstrate that the Deccan magnetism continued to affect western India for at least 1-2 Ma after the peak Deccan eruption during the K- T boundary. On the basis of published geological and geochemical information along with our Palaeomagnetic results, these intrusive bodies could be attributed as the feeders for the uppermost formations of Wai subgroup of the Western Ghat Deccan basalts, or to the major geodynamic events such as the opening of the Arabian Sea and the rifting of the Seychelles-Mascarene oceanic plateau, which occurred soon after the cessation of the Deccan volcanism. Rock magnetic experiments such as the Lowrie-Fuller test along with low-temperature magnetic susceptibility and isothermal remanent magnetization (IRM) measurements have identified single domain (SD) type titano-magnetite as the major characteristic remanent magnetization carrying magnetic mineral in the dyke samples.
Full text: Specific activity of natural (238U, 232Th, 226Ra and 40K ) and anthropogenic radionuclide (137Cs) in 109 soil samples collected from 11 districts in Phathalung province have been measured and analyzed. Experimental results were obtained by using a high purity germanium (HPGe) detector and gamma spectrometry analysis system at Nuclear and Material Physics laboratory in Department of Physics Faculty of Science Thaksin University Songkhla Campus. Gamma ray radioactive standard sources 60Co, 137Cs and 133Ba were used to calibrate the measurement system. The KCl and two reference materials (RGU-1 and RGTh-1) obtained from the International Atomic Energy Agency were also used to analyze and compute the 40K, 238U (or 226Ra) and 232Th specific activity in all soil samples. The IAEA/SL-2 was also used to evaluate the specific activity of 137Cs in all soil samples. The measuring time of each sample was 10,800 seconds. It was found that specific activity ranged from 148.17 to 11276.78 Bq/kg for 40K, 58.29 to 518.45 Bq/kg for 226Ra, 8.40 to 236.19 Bq/kg for 232Th and 0.00 tp 12.39 Bq/kg for 137Cs with mean values of 3573.35 ± 203.89 Bq/kg, 135.89 ± 6.71 Bq/kg, 76.34 ± 5.32 Bq/kg and 1.05 ± 0.70 Bq/kg respectively. Furthermore, absorbed dose rate in air (D), radium equivalent activity (Raeq), external hazard index (Hex), and annual effective dose rate (AEDout) of this area were also evaluated by using the mean values of specific activities of the 40K, 226Ra and 232Th. Moreover, the experimental results were also compared with Office of Atoms for Peace (OAP) research data, Thailand and global radioactivity measurement and evaluations. The radioactive contour maps of specific activities of natural and anthropogenic radionuclides from this study were created by using the program ArcGis Version 9.2
The technical part of this thesis describes the construction, the properties, and the experience from the application of an intelligent software trigger for the experiments on the production of positive kaons at the kaon spectrometer. The software trigger was applied in several beam-times. An essiential improvement in the yield of the registrated kaons was reached. The realization ensued by the application of standard components and represents a flexible and cost-efficient solution. The physical part of this thesis deals with the measurement of the double-differential cross section for positive kaons, positive pions, and protons in the system Ne+NaF at 1.0 GeV/u beam energy in the laboratory-angle range ofΘlab=44 ±4 . the beam energy of 1.0 GeV/u lies below the threshold energy for the production of positive kaons in the collision of two free nucleons of Ebeamthreshold=1.58 GeV. The momentum spectra of kaons and pions in the laboratory-momentum range from 270 MeV/c to 1140 MeV/c can be well described by a Boltzmann distribution in the c. m. system. The steepness parameter of the Boltzmann distributions amount to 64 MeV for kaons and 58 MeV for pions. The momentum-integrates cross section were determined from the extrapolation of the fitted Boltzmann distributions to: (dσ/dΩlab)ΔΩlabK+=58.6±9.2 μb/sr and (dσ/dΩlab)ΔΩlabπ+=169±17 mb/sr. The total cross sections were calculated under the assumption of the isotropy in the c. m. system from the fitted distributions: σ0K+=335±52 μb and σ0π+=939±96 mb. The mass dependence of the meson production cross sections in the symmetrc collisional system Ne+NaF was studied and compared with the results of the study of the heavy collisional system Au+Au
with the Hubble Space Telescope. Although none of them is distant enough to allow the detection of intergalactic He+ with HST, He+-absorption towards one of these objects, HS 1700+6416 was detected by the Hopkins Ultraviolet Telescope during NASA's Astro-2 mission in 1995. The first detection of intergalactic He+ was made in 1994 by a group of European astronomers in the quasar Q0302-002, cf. ESA Press Information Note 17-94 (7 July 1994).  An ion is an atom that has lost one or more of its electrons.  The ionization potential of hydrogen is 13.6 electron volt (eV), of neutral helium, 24.6 eV, and of singly ionized helium, 54.4 eV. In order to ionize the primordial hydrogen and helium atoms, photons of the indicated energies must be emitted by the first galaxies and stars. The corresponding photon wavelengths, all in the far-ultraviolet spectral region, are 912 A (91.2 nm), 504 A (50.4 nm) and 228 A (22.8 nm), respectively. The (Planck-)temperatures required are of the order of 32,000 K, 58,000 K and 127,000 K, respectively, which shows that the second ionization of helium cannot be done by the radiation from stars - they are not sufficiently hot. Thus He+-ions can only be ionized by the radiation from quasars. More information on ESA is available on the World Wide Web at http://www.esa.int ESO Press Information is available at http://www.eso.org/outreach/press-rel/. ESO Photos may be reproduced, if credit is given to the European Southern Observatory.